LOCUS       DAELTA010000001        69430 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.001, whole genome shotgun sequence.
ACCESSION   DAELTA010000001 DAELTA010000000
VERSION     DAELTA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.001"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            487..624
                     /gene="rpmH"
                     /locus_tag="KPG13_000001"
     CDS             487..624
                     /gene="rpmH"
                     /locus_tag="KPG13_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG0983756.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            702..1046
                     /gene="rnpA"
                     /locus_tag="KPG13_000002"
     CDS             702..1046
                     /gene="rnpA"
                     /locus_tag="KPG13_000002"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG0983757.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            1078..1329
                     /gene="yidD"
                     /locus_tag="KPG13_000003"
     CDS             1078..1329
                     /gene="yidD"
                     /locus_tag="KPG13_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG0983758.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            1361..2068
                     /locus_tag="KPG13_000004"
     CDS             1361..2068
                     /locus_tag="KPG13_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0983759.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            2037..2666
                     /locus_tag="KPG13_000005"
     CDS             2037..2666
                     /locus_tag="KPG13_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG0983760.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2778..4157
                     /gene="mnmE"
                     /locus_tag="KPG13_000006"
     CDS             2778..4157
                     /gene="mnmE"
                     /locus_tag="KPG13_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG0983761.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4255..6147
                     /gene="mnmG"
                     /locus_tag="KPG13_000007"
     CDS             4255..6147
                     /gene="mnmG"
                     /locus_tag="KPG13_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG0983762.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            6140..6859
                     /gene="rsmG"
                     /locus_tag="KPG13_000008"
     CDS             6140..6859
                     /gene="rsmG"
                     /locus_tag="KPG13_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG0983763.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            7022..7807
                     /gene="noc"
                     /locus_tag="KPG13_000009"
     CDS             7022..7807
                     /gene="noc"
                     /locus_tag="KPG13_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG0983764.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7942..8715
                     /locus_tag="KPG13_000010"
     CDS             7942..8715
                     /locus_tag="KPG13_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG0983765.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8717..9580
                     /locus_tag="KPG13_000011"
     CDS             8717..9580
                     /locus_tag="KPG13_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG0983766.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9613..10755
                     /locus_tag="KPG13_000012"
     CDS             9613..10755
                     /locus_tag="KPG13_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0983767.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10889..11464)
                     /locus_tag="KPG13_000013"
     CDS             complement(10889..11464)
                     /locus_tag="KPG13_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG0983768.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11724..13016
                     /locus_tag="KPG13_000014"
     CDS             11724..13016
                     /locus_tag="KPG13_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0983769.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            13121..13723
                     /gene="yedF"
                     /locus_tag="KPG13_000015"
     CDS             13121..13723
                     /gene="yedF"
                     /locus_tag="KPG13_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG0983770.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13796..14059
                     /locus_tag="KPG13_000016"
     CDS             13796..14059
                     /locus_tag="KPG13_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG0983771.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            14074..14259
                     /locus_tag="KPG13_000017"
     CDS             14074..14259
                     /locus_tag="KPG13_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG0983772.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14362..15558
                     /locus_tag="KPG13_000018"
     CDS             14362..15558
                     /locus_tag="KPG13_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0983773.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15772..16050
                     /locus_tag="KPG13_000019"
     CDS             15772..16050
                     /locus_tag="KPG13_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG0983774.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            16087..16521
                     /gene="ssb"
                     /locus_tag="KPG13_000020"
     CDS             16087..16521
                     /gene="ssb"
                     /locus_tag="KPG13_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0983775.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16540..16767
                     /locus_tag="KPG13_000021"
     CDS             16540..16767
                     /locus_tag="KPG13_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG0983776.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16910..17230
                     /locus_tag="KPG13_000022"
     CDS             16910..17230
                     /locus_tag="KPG13_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0983777.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17238..18194
                     /locus_tag="KPG13_000023"
     CDS             17238..18194
                     /locus_tag="KPG13_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG0983778.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            18205..20199
                     /locus_tag="KPG13_000024"
     CDS             18205..20199
                     /locus_tag="KPG13_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG0983779.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            20200..20649
                     /locus_tag="KPG13_000025"
     CDS             20200..20649
                     /locus_tag="KPG13_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG0983780.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20668..21996
                     /gene="dnaB"
                     /locus_tag="KPG13_000026"
     CDS             20668..21996
                     /gene="dnaB"
                     /locus_tag="KPG13_000026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG0983781.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            22201..22509
                     /locus_tag="KPG13_000027"
     CDS             22201..22509
                     /locus_tag="KPG13_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983782.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22591..23880
                     /locus_tag="KPG13_000028"
     CDS             22591..23880
                     /locus_tag="KPG13_000028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG0983783.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            24206..24281
                     /locus_tag="KPG13_000029"
     tRNA            24206..24281
                     /locus_tag="KPG13_000029"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24239..24241,aa:Lys,seq:ttt)
     gene            24288..24362
                     /locus_tag="KPG13_000030"
     tRNA            24288..24362
                     /locus_tag="KPG13_000030"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24321..24323,aa:Glu,seq:ttc)
     gene            24367..24442
                     /locus_tag="KPG13_000031"
     tRNA            24367..24442
                     /locus_tag="KPG13_000031"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24400..24402,aa:Val,seq:tac)
     gene            24476..24550
                     /locus_tag="KPG13_000032"
     tRNA            24476..24550
                     /locus_tag="KPG13_000032"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24508..24510,aa:Thr,seq:tgt)
     gene            complement(24933..25925)
                     /locus_tag="KPG13_000033"
     CDS             complement(24933..25925)
                     /locus_tag="KPG13_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0983784.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25934..26992)
                     /locus_tag="KPG13_000034"
     CDS             complement(25934..26992)
                     /locus_tag="KPG13_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG0983785.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            27117..28580
                     /locus_tag="KPG13_000035"
     CDS             27117..28580
                     /locus_tag="KPG13_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG0983786.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            28682..29479
                     /locus_tag="KPG13_000036"
     CDS             28682..29479
                     /locus_tag="KPG13_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0983787.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            29492..31594
                     /locus_tag="KPG13_000037"
     CDS             29492..31594
                     /locus_tag="KPG13_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG0983788.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            31662..32141
                     /gene="rlmH"
                     /locus_tag="KPG13_000038"
     CDS             31662..32141
                     /gene="rlmH"
                     /locus_tag="KPG13_000038"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG0983789.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32290..32601
                     /locus_tag="KPG13_000039"
     CDS             32290..32601
                     /locus_tag="KPG13_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0983790.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            32649..32966
                     /locus_tag="KPG13_000040"
     CDS             32649..32966
                     /locus_tag="KPG13_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0983791.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            33179..33880
                     /locus_tag="KPG13_000041"
     CDS             33179..33880
                     /locus_tag="KPG13_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0983792.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            34104..35405
                     /locus_tag="KPG13_000042"
     CDS             34104..35405
                     /locus_tag="KPG13_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0983793.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            35542..36636
                     /locus_tag="KPG13_000043"
     CDS             35542..36636
                     /locus_tag="KPG13_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0983794.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            36638..37771
                     /locus_tag="KPG13_000044"
     CDS             36638..37771
                     /locus_tag="KPG13_000044"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG0983795.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            37789..38898
                     /locus_tag="KPG13_000045"
     CDS             37789..38898
                     /locus_tag="KPG13_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG0983796.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            38963..39703
                     /locus_tag="KPG13_000046"
     CDS             38963..39703
                     /locus_tag="KPG13_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG0983797.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            39708..40700
                     /locus_tag="KPG13_000047"
     CDS             39708..40700
                     /locus_tag="KPG13_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG0983798.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(40888..41769)
                     /locus_tag="KPG13_000048"
     CDS             complement(40888..41769)
                     /locus_tag="KPG13_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0983799.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            42042..43430
                     /locus_tag="KPG13_000049"
     CDS             42042..43430
                     /locus_tag="KPG13_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983800.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            43458..44411
                     /locus_tag="KPG13_000050"
     CDS             43458..44411
                     /locus_tag="KPG13_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0983801.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            44471..44695
                     /locus_tag="KPG13_000051"
     CDS             44471..44695
                     /locus_tag="KPG13_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983802.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            44688..45200
                     /locus_tag="KPG13_000052"
     CDS             44688..45200
                     /locus_tag="KPG13_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG0983803.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            45232..46086
                     /locus_tag="KPG13_000053"
     CDS             45232..46086
                     /locus_tag="KPG13_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG0983804.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            46620..47414
                     /locus_tag="KPG13_000054"
     CDS             46620..47414
                     /locus_tag="KPG13_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0983805.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            47518..48324
                     /locus_tag="KPG13_000055"
     CDS             47518..48324
                     /locus_tag="KPG13_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0983806.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            48386..48907
                     /locus_tag="KPG13_000056"
     CDS             48386..48907
                     /locus_tag="KPG13_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0983807.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            49322..51409
                     /locus_tag="KPG13_000057"
     CDS             49322..51409
                     /locus_tag="KPG13_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0983808.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            51424..51711
                     /locus_tag="KPG13_000058"
     CDS             51424..51711
                     /locus_tag="KPG13_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0983809.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            51738..53009
                     /locus_tag="KPG13_000059"
     CDS             51738..53009
                     /locus_tag="KPG13_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG0983810.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            53067..53666
                     /locus_tag="KPG13_000060"
     CDS             53067..53666
                     /locus_tag="KPG13_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0983811.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            53671..54576
                     /gene="dapA"
                     /locus_tag="KPG13_000061"
     CDS             53671..54576
                     /gene="dapA"
                     /locus_tag="KPG13_000061"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0983812.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            54682..55314
                     /locus_tag="KPG13_000062"
     CDS             54682..55314
                     /locus_tag="KPG13_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG0983813.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            55791..58298
                     /locus_tag="KPG13_000063"
     CDS             55791..58298
                     /locus_tag="KPG13_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0983814.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            58640..60802
                     /locus_tag="KPG13_000064"
     CDS             58640..60802
                     /locus_tag="KPG13_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG0983815.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            60807..61424
                     /locus_tag="KPG13_000065"
     CDS             60807..61424
                     /locus_tag="KPG13_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0983816.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            61613..61957
                     /locus_tag="KPG13_000066"
     CDS             61613..61957
                     /locus_tag="KPG13_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG0983817.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            62527..63393
                     /locus_tag="KPG13_000067"
     CDS             62527..63393
                     /locus_tag="KPG13_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0983818.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            63506..63910
                     /locus_tag="KPG13_000068"
     CDS             63506..63910
                     /locus_tag="KPG13_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG0983819.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            64074..64523
                     /locus_tag="KPG13_000069"
     CDS             64074..64523
                     /locus_tag="KPG13_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0983820.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(64679..65056)
                     /locus_tag="KPG13_000070"
     CDS             complement(64679..65056)
                     /locus_tag="KPG13_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0983821.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            65318..66235
                     /locus_tag="KPG13_000071"
     CDS             65318..66235
                     /locus_tag="KPG13_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG0983822.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            66719..68137
                     /locus_tag="KPG13_000072"
     CDS             66719..68137
                     /locus_tag="KPG13_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG0983823.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            68213..69025
                     /locus_tag="KPG13_000073"
     CDS             68213..69025
                     /locus_tag="KPG13_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0983824.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT        27407 a         7439 c        12264 g        22320 t
ORIGIN      
        1 aagttataaa taacataaac atacaataaa tggtgattat aactagagta ttgtcagtaa
       61 tcttaaaagt tatacacaaa agtatccaca agctgtgaat aatttctaat aagttgataa
      121 catttatgca cattatcaac aagtatattt ataatatcaa aatatatgtc aaacttcaat
      181 gatttgtaac taatttatcc acaatattaa ctaattgttg ataaaaatat aaacatgtta
      241 aaattacttt atttgtgtat ataattgaaa aaaataattt agaataaaat taaatagatt
      301 tttactagta ttttcaataa aaaaaataca taatatatat atggtagatt ttgtgatata
      361 agcgtcccaa aaactcgttg acaacagtag taaaagtatt tataattata tgtgttatgt
      421 ttactttgag ggtaaagtta ttatttttat aaacggaaaa taaaatctga aaggcggtgc
      481 aaaataatga gtaaaagaac ttatcaacca aaaaagagac aaagaagtaa agaacatggt
      541 ttcagaaaaa gaatgaagac gtctaacgga agaaacgtgt taaagagaag aagagctaaa
      601 ggaagaaata gattaactca ttaattacaa aagggccgct gcaaaggtgg cctttttgtg
      661 taaataatta caattcatag ctgtgtttat ggaggttaac tatggacttt aataggacta
      721 aagggttgaa aaaagactct gattttagaa aagtatataa acacggcaaa tcttttgcaa
      781 acaaatattt agtaatatat atacttaaaa ataaatcaga ttatagtaga gtaggtattt
      841 ctgtttcaaa aaaagtagga aaagctatta ctaggaatag agtaagaaga ttaataaaag
      901 aagcctacag attaaatatt gatgaaaaaa ttaagcctgg atatgatata gtatttatag
      961 caagggtatc tagtaaggat gctactttta aagacataga caagtctata aaaaatttag
     1021 ttaaaagaac ggatatttct atttaattgt atgctataaa tatggaggtt aatgactttg
     1081 aataaaataa tttatatatt aaaggaaata agtaagtatt tatcgaattt atgtatttat
     1141 ttagtgagat tttatcaaaa gtatatatct cccttaaagg gacctacctg tagattttat
     1201 ccaacttgct cacaatattc aatagaggcg tttaagaaat atggatttgt aaaaggtatg
     1261 tatttaacga taagaagaat tttaaaatgt catccttttc atccaggagg ttacgaccct
     1321 ctaaaataaa agagagtaac tattaggagg tataaaaaaa ttgaatataa taggaaattt
     1381 cttaggattg attcttaagg taatatttga atttgttaat cattatggta tttctataat
     1441 actttttacg atacttgtaa agattatttt attaccatta acaattaagc agactaagtc
     1501 aacaaaggca atgcaagata tacaacctcg tataaaagag atacaagaaa aatataaaaa
     1561 taagccagaa aaacaaaatg aagaaattgt taagttatat ggagaagcta aaattaatcc
     1621 actatctggt tgtttacctt tattaataca gtttcctata ttaataggat tatttagcgt
     1681 tttgagagaa ccagttgctc atggcgtttt tgcaaacaaa gctgcatttt tagcagctga
     1741 caatgggttt ttatggataa aaagcttaac atcaccagat tatgtattag cagtattttc
     1801 aggagcaagt gcatatgtca tgcaaaaagt aatgacacca aaagatcagt tacaaggctc
     1861 tatgaaagta atgacatatg taatggcagg tatgtctttc tattggggat ttatattccc
     1921 agcaggactt acattgtact ggacagtaag taacttattc tcaatagctc aatattactt
     1981 aataatgaat cctttaaaag ctaaactggc tgctaactct aaggaggaaa aagttaatga
     2041 gaaaaagcct aaaattaata aaaagtaaga gtaaagagga agctataagt aaagctatag
     2101 cagagttgaa tttaaaagca gaggatatag aagtagaaat acttgaaaat ccaagtaaag
     2161 ggtttttagg ttttataggt gctaaagatg gtacttatga aatttttgtt atcgagaggg
     2221 aagtagatgt agctaaaaat tttatagagg taatgttgaa aaatgcaaat gttgatgcca
     2281 aagtcaatgt atctcaaaaa gataatttaa tcatggttga tattgaaggc aaagaagctg
     2341 caagtttaat tggtagaaga ggggaaactt tagattctat tcaatttttg actggattag
     2401 ctcttaataa aataaataaa gattctcata caagagtact tgtagatata gaaaattata
     2461 gatcaaaacg agaagaatct ttaataagat atgctaataa agttgctaga gaagtagcga
     2521 aaacaagaaa aactaaaaaa ttagattata tgaatccata tgagagaaga ataatacatt
     2581 cagctcttca aaatgacaaa tatgtaatta catatagtga aggaaccgat ccatatagaa
     2641 gattagtaat tgagtgtaaa agataaagtc aacctcctaa acattagggg gttttatttt
     2701 ttaatattat taaatcatta atgattggtt aaatgtaaat ctatatgtaa aaaattatga
     2761 attaagaggt gataaatttg tttatagatg atacaattgc agcaatagct acagcacctg
     2821 gagaaggtgg tataggtata cttagaataa gtggagaaaa agctttgaag gttgcagaag
     2881 aaatctttaa atctatgtct ggaaagagta ttgaagaata caataagaga acattaatat
     2941 atggaaatat agtggataat gaaaatataa ttgatgaagt tttattggca tatatgaaag
     3001 gaccaaattc ctatacagga gaagatgtta tagaaattaa ttgccatggt ggatttattt
     3061 cggtaaagaa gatattagaa ttaatactat caaaagatgt tagattagca gaggctggtg
     3121 agtttactaa aagagcattc ttaaatggaa gaatagattt atctcaagca gaggctgtaa
     3181 ttgatgttat aaaagccaaa actgatatag ctcatgaagt tgctcaaaat caattagaag
     3241 gttctttatc aaagaagata agagaattga gagataaagt cactgagata ttagctcatg
     3301 tagaagttgc tatagattat cctgaggaag atattgagca tataacatat caaactctta
     3361 aagaaaaaac tgatgaactt aaaaaagaca ttaaaaaatt atatgataca gctgaaagtg
     3421 gaaaaatact tagagaaggc ttaaagacgg taattgttgg gaaaccaaat gtaggtaaat
     3481 cttcactact taattctatt ttaggagaga atagagcaat agttactgat ataccaggaa
     3541 ctactagaga tgttatagaa gagtttgtaa acataaaagg tataccttta aaaattgttg
     3601 acacagcagg tataagagat actgatgata ttgttgaaaa aataggtgtt gaaaagtcta
     3661 aagaatcatt tactagtgca gatttgatag taatggtttt agatgcatct agaaagttgt
     3721 cagaagaaga tatagagata ttagaaaaac ttaaggataa acaaacaata gtgctgttaa
     3781 ataagaatga tttgaaacaa gagattgaag aagaaaaaat acttaaatat gtagaaaata
     3841 atagtataat aaaaatatca gctttacaac aagaaggaat agaagaattg caagataaaa
     3901 tagagtctat ggtatataaa ggtagtatca aaaataattc tagtttagta gtaacaaatt
     3961 caagacataa agatgcactt agtaaagcat ataaatctgc aacagatgct ttaattgctt
     4021 tggaacaaag tatgccattt gactttgtag aagtcgattt aaaaaatata tgggactatc
     4081 ttggatatat taatggtgat acagtaacag aggatttact agataatata ttccataatt
     4141 tctgtattgg taagtaatgt acaaaagtaa acttgttttt attatagaga tttttaaaaa
     4201 atttgaagtg tttctcatga aacattaatt ttgaaaagaa aggaattata aatgataaag
     4261 tttgaagcag gaaaatatga tgttatagta gtcggagcag gacatgctgg atgtgaagct
     4321 gctttggcta ctgcaagaat gggatataaa actttgataa taacaatgtc tttggattcc
     4381 atagcattaa tgccttgtaa tccatcaatt ggagggactg gtaaaggtca attagttaaa
     4441 gaaatagatg ccttaggtgg tcagatggga ttaaatatag acaagacata tatacaaagt
     4501 agaatgttaa atactgccaa aggtccagcg gtacactctt taagagcaca agcagataaa
     4561 tttaaatatc atgaagaaat gaaaaagaca ttagaagatg aaccaaatct tgatatagct
     4621 atggatgaag ttgtagaaat acttcatgaa ggaaatgttg taataggagt tggaactaaa
     4681 ctaggatgtt catttaaatc taaagctgta atattggcca ctggagttta tctgaactca
     4741 aagatatata tgggtgaagt tgcattttat gaaggtccta atgcattggg gtatgcaaaa
     4801 tatcttacag atagcttagt tgagcttggc cttagaatga gaagatttaa gacgggaact
     4861 ccagcaagag ttcatagaga tagtatagac ttctctgtaa tgtctttaca agagggtgat
     4921 gaaaaagtaa ctccattctc atttatgaat gaaaatatag aaaaaaaaca agaaccttgt
     4981 tatttaacaa gaactacaga agaaactcaa aaagttatac tggataattt aaaaaggtct
     5041 gctatgtata gtggagtaat agaaagtact ggtccaagat actgtccttc tatagaagat
     5101 aaagtagtaa gatttagtga taaaacatca catcaattat ttatagaacc agaagggtta
     5161 aatactaaag aaatgtatat acaaggaatt tcaactagtt taccttttga agttcaatta
     5221 gatatgtaca aaactataaa gggacttgaa aattgtaaga taatgagacc tgcatatgct
     5281 attgaatatg attgtgttga cccaactcaa ctaaaaataa gtttggaaat aaagggtgtt
     5341 gagaacttat ttagtgcagg gcaatttaat ggaacttctg gatacgagga agcagcagct
     5401 caagggctta tggctggtat aaatgcagtt agaaagatag aaggaaaaga gccatttgta
     5461 ttagaccgtt cagaagctta tataggtgtc ttattagatg accttgttac taaaggaact
     5521 aacgagcctt acagaatgat gacatcaaga gcagaatatc gactatattt aagacaagat
     5581 aatgcagata tgagattaac tcaaaaaggt tatgatatag gattagttaa aaaagataga
     5641 tatgaaaggt tcctaaataa aaaggctgct gtagagaaag aatttgaaag actaaaaaat
     5701 gaaagagtta caccaaaaga agtaaatagt ttgttagaag aaaaaggagc aacaccaata
     5761 aaggttggaa tatctctata tgaattttta aagagaccag aggttactta cgaattgctt
     5821 gaagaattag ggaaaggcgc tggagaagat gtttcaagag aagttaaaga acagtgtgta
     5881 ataataacta aatatgaagg ttatatagag aaacaattga aacaaataga ccaatttaaa
     5941 aaacttgaga ataaaaagct ggatgagaaa ataaattatt catctataga aggtcttaga
     6001 ttagaggcta gacagaaact agatgatata aaacctattt caatagggca agcatctcgt
     6061 atatctggtg tttctccagc tgatatatca gttttactta tatacctaga acaaatcaga
     6121 agaactagag gtggcaaagg tgaataatat agaacttctg aaaaacggga ttgaaggttt
     6181 taatatagat acgaatgaca gtatgttaga aaaatttaaa atctatagag agatattagt
     6241 tgactggaat aaaaagatga atcttactgg aatagaagat gaaaaagaag tgtatataaa
     6301 acatttttta gactctattt cagctgtaaa gaatggatat ataaaaaatg ggatgtctat
     6361 aatagatgtt ggtacaggtg ctggttttcc tggaatacca ttgaaaatat gtttagatag
     6421 cttagaactt actttgttag attcgttaaa taaaagaata aactttttag aggaagttag
     6481 tagagtacta gagttagata atattacatt tatacatgga agagctgaag attttggtaa
     6541 agatgaagac tatagggaaa aatatgatgt tgctacggca agagctgttg ctggattacc
     6601 gattcttatg gaattttgtg taccatttgt aaaagttgga gggtatttta tttgcttaaa
     6661 aggtccgaat gctaatttag agctagaaga atcacaaaag gctattgatg ttttaggatt
     6721 ggagtatatt gaaaaaatag atgtagagtt accagaggta gatttaaatc ataatatatt
     6781 agtttttaaa aaagtgatgg aaacaccagt taagtaccct agaaaagctg gtaaaccagc
     6841 caaaaatcct attaagtaat gaaaaattta ttattggtaa gcttaattat ttctcaggaa
     6901 ataattaagc ttactgtaaa aaaacaaaaa taaaggaatt attaaagtag atgcagaaat
     6961 aaatttgtat gtacatatag agaacaaaaa taatttatat ctaaaataag agaggtatgt
     7021 tatggaagat aaaagagtta tggagatacc tatagaagat gtagttccaa atccatatca
     7081 accaagaaaa atattttcac aagtttcatt ggaggaattg agcaattcta taaaggtata
     7141 tggtataatt caacctataa cagttagagc aaaagatggc aaatatgaac taattgcagg
     7201 agaaagaaga ttaagagcag ctaaattagc agagcttaaa accataccag caattataaa
     7261 taatatgaac gatgaatctt ctgctgttct agcgttatta gaaaatttgc aacgagaaga
     7321 cttaaatttc attgaagaag caattggtta tgaaaactta ataaaagaac atgcttttac
     7381 tcaacagcaa ttagcagaaa aattgggcaa aaatcaatct actgtagcaa ataagcttag
     7441 aatattaaag cttccaaacg atataaaaat gaagctaata gagaataatt taacagaaag
     7501 acatgctaga gcatttctta aactaccaag tgaagattta atgcaaagtg tgttagacaa
     7561 agtcataaaa aatgaattga ctgttaaaaa aacagaaaaa ttgatacaag atgtattaga
     7621 agaaactaaa gttcaagaag agcctgataa aaaacagaat ataaagggtg ctatgagtat
     7681 aagaatttac ataaatacta taaagcaggc ctttgatgct atatcaaata caggaattga
     7741 tgctaagtat aatgaaatag ataagggaga ttatatggaa gttgttgtaa aaatccctaa
     7801 aaaatagaga ttactgctta ccagtaatct ttttttgtaa tataagcctt gtaatataaa
     7861 ttgatttata gacattattt atacttaaaa aacacctatt caacatttaa aattaattta
     7921 attcttaaag gggggaacat catgggcaaa gttatagctg tattcaatca aaagggtgga
     7981 gtcgggaaaa ctacaacaaa tgtaaattta agtgctagct taggaacttt gggaaagaaa
     8041 atattagtat tagatttaga cccacaagga aatacaacta gtggatatgg tattaataaa
     8101 aacgaagtag aaaatacaat ctatgaaatt atgttagatg ggttacatat taaagaagca
     8161 ataataagta ctgaatttga gaatgtagat gtagttccat cagctactga actatcaggt
     8221 gcagagatag agcttacatc taaaactaat agagagtata ttttaaaaaa ttccataaaa
     8281 gcggtaatag atgaatatga ttatatattt ttagattgtc caccctcact ggggatgctt
     8341 acaataaatt gtttaactgc tgtagatagt gttttaattc caatacaatg tgaatattat
     8401 gctttagaag gtgttagtca attaatggag actataaaat tagtaaaatc aagattaaat
     8461 gctgatatag aaattcaagg tgttgtttta agtatgtttg atggaagagc taatttatca
     8521 atacaagtag ttgaagaagt taagaaatac tttaaaggta gtgtatatac aactttaatt
     8581 ccaagaaatg taagacttgc agaagctcca agtcatggaa aacctgtaat ttattatgat
     8641 aaaagatgta gagggtctgt agcttatttg gagctagcag aagaatttat agatttagaa
     8701 gaggaggaat ggtagtatgg agaataagtc taaaagaagt aatagattag gtagaggtct
     8761 tagtgcattg ataccagaaa ttaaaggaga aacttcagaa aaagaaatag tcaacataga
     8821 tatagacaaa atatatccaa acgaagttca acctagaaag caatttgatg aagaaaagat
     8881 aaaagtattg tcggattcta taaaaaatta tggtgtttta cagccaatag tggttaaaat
     8941 ggatgaaaat aataaatata tgataatagc tggagaaaga agatttagag catcaaaact
     9001 tgcaaataaa aatcaaatac ctgctataat taaagatata gatatgaaag atataatgga
     9061 gatagcctta attgaaaact tacagaggga agatttaaat tcaatagaag aggctctagc
     9121 atataagagt ttaatagagc attacaatgt aactcaggaa gaaatatcag aagctgttgg
     9181 gaagagtaga cctcatataa ctaatacact aagactttta aatcttggac aagatgttat
     9241 agaaatgata gacagtggta gaatcacagc aggccatggt aaggctttat tgagaattgc
     9301 agataaagat ttgcaacttc aaatagcgaa aaaaatagaa gaagaagaac tttctgtaag
     9361 agaagtcgaa aatatagcta aaaaaatatc tgaaaataaa caagaagaac ctaaaaaatc
     9421 taaacctaag gatgtattta ttttagatgt tgaagataaa cttagaaata tatttggaac
     9481 aaaggtgaat atttctaaag gaaagaaaaa aggtaaaata gagatagaat actataatga
     9541 tgatgattta aatagtattg tatcaatgct tcttgaatag aacaaaaata taaaacagag
     9601 aggaagattg ttatgattta tttagataat gctgcaacta catatccaaa accagaaaga
     9661 gtgtataatg ctgttttaga ttgtatgaaa aattattgtg caaatccagg tagagcaggc
     9721 cataaacttg ctatgagagc tgctagggag atatatgata caagagagaa tatagctaag
     9781 ttgttcaatg taagtaatcc tatgaatata gtatttacat ctaatgcaac agattcttta
     9841 aatttagcga taaaaggtgt attacaagaa ggagaccatg taataacaac gtctatggag
     9901 cataattcag taataagacc tataaaagca ttggaaaaaa ggggtattga aaatactgta
     9961 gtaaaatgcg attatgaggg atttttagat tatgaagatt tagaaaaatc aataaagagt
    10021 aatactaaac tgattgtaac aacacatgcg tctaatgtat gtggaacttt aatagatata
    10081 aaaaaagttg gagaaatagc taaaaaacat aatatactat ttttagtaga tgcatcacaa
    10141 acagcaggtg tttatgatat agacgtaaat gaatgtaata tagatatgtt ggcaatgcca
    10201 ggtcataaat gcttatttgg acctcaagga acaggtatat tatatgtaag agaagggttg
    10261 aacttaaata tattaaaaga aggtggaaca ggtagtaaat cagaagaaat agttcaacca
    10321 gagttattcc cagataaata tgaatcagga actcataata ctccaggtat agcaggtctt
    10381 aaccaaggta tactatttat atttgaaaga ggaatcaaca atataagaca acatgaagaa
    10441 gaattatgtc aatatatgat agacaaatta gaggaagtac cagatataaa gatatatgga
    10501 ccaaaggaca gtaaaaaaag agcatcagta atagctctaa atataggaga tatggattct
    10561 ggagaggtta ctttcttatt agatagtgat tataatatag cgactagatc aggaatacat
    10621 tgttcaccac tagcacatac cactttaggt actttaaaac aaggtgcagt aaggtttagt
    10681 ataggatatt tcaacacaaa agatgagata gataaagcag tggaagcttt gaaaaaaatt
    10741 tcaaaaaata aatagcatga gatttaataa tatagaaata aaaaaagggg atatttcaaa
    10801 ataaaaattt gaatcatctc cttttttgta caaaaaagaa aaggggttcc taagaacctc
    10861 taatctattt gataatagtt tcaagaaatt actttaagta atctccttct tcaagaacta
    10921 tatgaccact ttcgtaatta gaaccatcaa cagtaatttt tatttgttca acattcatat
    10981 tttttatgta agtacgagca actgaatcaa gcattaaagt ttctgcatca ctacctaagt
    11041 ttgactttgt aaagtcagag ctaacatcta atataccagt ttttacacca tcaatgtctt
    11101 ttgtagtaaa tgaatttaat tttgcagatt ctggaacaac tttaagtttt tgaagttctt
    11161 caaataaagt gttctcatca attttattta aatcaacttt attttcaatt aaatctgtct
    11221 tatctacatc aaaagagtaa atagtagctg attgagtttt agtttcttct tttggagtag
    11281 gtttagcttt gttattagga acctcttttt cagtattgtt ttcttctttt ggttgctcaa
    11341 catttgtatt gctcttagtt tcttttttag atgaatcact tccattttga gcattagtac
    11401 aaccaactgc gaatatagat atagataata tacttaacac taacattatt ttcttatttt
    11461 tcataaaaat ttctcctctc ttaaaaacta ttcttatata taattatagt cgttatgagc
    11521 aaatatatga atacaaaata gtaacaaatt taaaaaatga tttatttaaa ttaataaaac
    11581 tcaaaataaa ctaaatttta gtcattttta tgggaaatca tttaatgtac agtgaaaata
    11641 ttgtaaaata aaagtagtgt ttttaaaata aatataagtt ataattatat tataaaaaaa
    11701 tgtcttcaaa aggtgggaat agtatggaaa ttttgagctt aggagaaaag attaaaaaat
    11761 taagaaaaga aaaaaacatg acattaaaag aattagctgg agataggata acagcagcac
    11821 aaataagcca tatagagagg gataaatctc atactagtta tgaactgttg gaatatctag
    11881 gcgataagct agatgtaagt gtagaatact tattagagac caaggaaatg caatcaaaaa
    11941 agataactga taatttaata ctacagagcg aaatttatat aaaagaaaat gaattaaaca
    12001 aggctgagga ccaaataaaa gaagttttag atatatgtaa gagatacgat ttaatggaga
    12061 actatggcag atgtaatttt ttaatggcta atattaattt gaaaaaatct aattataatg
    12121 atgctataga aaactttgaa aaggcattgt acttctttat aaaaaatagt gatagtgaaa
    12181 atatacttaa gtgttattta aacataggga aaatatatat gcaggaagac ttttataaag
    12241 gtgctataag ccactttaat tttgcagagg aaattttgtc agagaataaa tttgaggatg
    12301 ttaacataca caaggaacta tatagtaaga tggcatattg ttacataaaa ctagataatc
    12361 caaatatgtc attgaagtac atagaaaaga taaatgaaat agataataga aatgatagaa
    12421 aagaagatgt cgatattttg gtgttaaaag caaataatat gcttgagatt ggaaaatatg
    12481 aagaatcaag agattacttt aaaaaagcac taaaaatact tgagagtgag gataataaaa
    12541 ctggattagc aaatgtgtat ttaacaattt gtgatgttta cagaaaaata ggtgaaacag
    12601 atagagtact tgaatattca caaaaggtat atgacataaa gaaaaatgat gaagatgaat
    12661 acatgatgtc aggattattt aagataatag aagcatatat agataaagaa gattatgatt
    12721 tagctagaaa atattgtaag atagccttag cttcatcaat aaaaaataaa aataaattca
    12781 atgaatataa agcattgaga ttctatgcta atatgtataa aaatcagaat gaagtaactc
    12841 ttgcaattga atatctgatt aaatgcataa aaatagtatc agatttaggc aacaatagaa
    12901 tattggccaa tttatatata gacttaggtc aattatattc aaatatatca aaagaaaaag
    12961 agttagaata ttatcaaaaa ggtgtattta tgtacaaaaa tttagaaata atgtagggta
    13021 aataaaccta taagggtata gaaacaatct ataacaagca agataatatc tatgataaaa
    13081 tataaattgt atgaatattt ttaaggagat gaatcagaca atgagtataa aaatagatgc
    13141 gagagggctt gcttgtccta aaccagttat aaatacaaaa agagaattgg ataacctaga
    13201 agaaggtgtt gtagttacaa tagtcgataa tgaaacagct aaggagaata tattaaaact
    13261 tgctaaatct ttaagttgtg aagcaaatat tgttgacgaa aaagagaatt ttatatctat
    13321 agaaattaga aaaggtaaaa atatactaga taaacaaaat gttgtagata ataaagaaag
    13381 tgaattagac aatacatgta tatttatatc ttcagataag atgggacttg gaaatgacga
    13441 attaggaaaa gtgttaataa aaggatttat atatacatta acagaatcta aaccttatcc
    13501 taagtatgta cttttagtta atggaggagt aaaattaagt gctgaaaatg aagaaaccat
    13561 agaaaatcta aaaatacttg agaatatggg tgtagaagtt ttatcttgtg gtacttgttt
    13621 agattattat aatttaaaag acaaattaca agtaggtaca gttacaaata tgtatactat
    13681 agttgagaca ttaaagaatg catcaaatac catttctata taatgtatgt ttgtttaata
    13741 atataaaatt aataataaag attaactcaa atagatagtc agaaaggatt aagtaatgaa
    13801 tcaaatgtat atagtgtcat ttaattcgac acatcatgcg ataagatcag aaaagttatt
    13861 tggtgaaaat agtctaaaag taatggctct tccaacacca agagaaataa cagcaagttg
    13921 tggaatatca attaaatttt cttttgaaga tatggaaaag ataaaaacta ttttagttga
    13981 aaataatgta gatataaaag gaatttattg tataagtaaa ttagaaaatg gctcaaaaga
    14041 agtagagaag ttagattagg aggctaatta tttatgccaa tggatttaaa aataggtgat
    14101 attgttgaat taaaaaagca acatgcatgt gggtgtaaag agtttgaaat cataagaacg
    14161 ggaatggata taaagataaa atgtactaaa tgttcaaggc taataatgct tgatagagag
    14221 accttagaaa agagagtaaa aaagctaata aaaaagtgat aaaaatttaa cacttaaaaa
    14281 atgtcaaata ccgtatatta atatccaaaa ttttggtata ataagaattg aagaaaacat
    14341 tttcatgggg gtgctttaat tatggcagtt aaatatgcaa aaagaatgca aggattacaa
    14401 ggatcagaaa tacgtgagct tttaaaactt actcaacaac cacaaataat atcttttgct
    14461 ggtggaatgc cggctccaga gttatttcca gttgaggaaa tgaaaaaagt atcagttgca
    14521 gtgcttgaag aaaatggaag atcagctatg caatatacta caacagaagg atatgaacca
    14581 ttaagagaaa aaatagcagc tagaatgaat gataaaaaca aaactaatgt aaacaaagat
    14641 gatatattag ttacaagtgg ttctcaacaa ggtctagatt ttgcaggaaa agtatttatt
    14701 gatgagggtg atgtaatttt atgtgaaagt ccatcttaca taggagctat aaacgcattt
    14761 aaatcttatc aacctaaatt catagatgtt ccaacagatt ctgatggaat gataatggaa
    14821 gaattagaaa agatactaga aacaactgat agaataaaaa tgatttatgt aatacctgac
    14881 ttccaaaatc caacaggaag aacttggcca cttgaaagac gtaaaaaatt catggaaata
    14941 gtaaataaat ttgaaatacc agtaatagaa gataatccat atggagattt aagatttgaa
    15001 ggagagactt taccatcttt aaaatcaatg gatacaaaag gattagtaat attcttagga
    15061 actttctcta aaatattctg cccaggatac agattaggat ggacttgtgc ttctccagaa
    15121 attttatcta agtttaactt tgcaaaacaa ggtgcagact tacaagcatc aactatatct
    15181 caaatggaag taagcaaatt tatggatatg tatgacctag atgcacatgt tgacaaaata
    15241 aaagcagttt atgttaaacg tagagatgta atgcttaaga ctatggaaga agagttccca
    15301 gaaggattag tatttactca tccagaaggt ggattattta catgggtaga gcttccaagt
    15361 aatttaaatg ctaaagaatt aatgccaaaa tgtttagata agaatgttgc ttatgttgct
    15421 ggaggaggat tcttccctaa tggtggtaga gaaaatactt tcagacttaa ctattcaaat
    15481 atgccagaag aaaaaataat agaaggtata aaaaatatag cagctgtatt aaaagaagct
    15541 atgggtgtag aagcttaatt aaaaataata acttatatat aatacaaggg atggttgttg
    15601 ccatcccttg tttgtttgaa aagtatataa ttcaccttat actaaacttt gtccaaaatt
    15661 taaattgaca gaaataagaa taaatgataa aatgtgtagg tatgagattt tagtctcatt
    15721 tctctgctct aagagtgtag agccgttaag tccaaaggga ggtgaattat agtgagaaat
    15781 tatgaattag tttatgtggt aaagccaaac tctgatgaag aagtaagaga ggctatacta
    15841 aacaaagtta aggaagttgt tgcaactgac ggagaaatag ttaaagttga tacttgggga
    15901 actaaaaaat tagcttatcc aatagctaag tttacagaag gtttctatgt attagttaac
    15961 ttcaaatcag cagtagacgt tcctaaagag atagacagaa acttaaagat aaatgaaaac
    16021 gtaataagac acatgatagt tgttgcttaa taactggttt tattataaaa aataggtggt
    16081 gtttttatga atcaagttgt attagtggga aggttgacta gagaccctga attaagatac
    16141 atcccaggta caggaacacc agtggcatcc tttacaatag ccattgatag agactatgta
    16201 aaaaaagatg gatctaaaga aactgatttt atacctgttg aagtaatggg taaatctgct
    16261 gaattttgtg ctaactacat aactaaaggt agattagtag cacttcaagg atctataaga
    16321 gtagacaatt atcagacaca atcaggtgaa aagagaactt ttacaaaagt aagtactaga
    16381 tcagtacaag ctttggatag taagaataaa tcggaaaatt catatagaga gagtgctcca
    16441 gcttttgagc caagctttga acctcaaggt ttagacccac aaggtttcca agctatagat
    16501 gatgacgata taccatttta aaaaggaggg aaaaaagtca tgatgaataa aaaaagacgt
    16561 aagaaaaaaa gagtttgtca attttgtgct gacaaaaacg ctaaaataga ttacaaaagt
    16621 actcaaagat tacaaaagta tataactgaa agaggaaaga tattaccaag aagaatatct
    16681 ggaacttgtg ctaagcatca aagagaatta acagttgcta taaaaagagc gagaaatata
    16741 gcacttttac catatacatt agactaaaaa aatgagcctt gtgctcattt tttattatat
    16801 gtatttatat atctaaatga ggcgagctat aaagctcgtt tttttgttta tatacttaaa
    16861 tatattatta tttatgatat aatattatat attaaggagg gttgtaaacg tgaaaataga
    16921 taaaggttct gagataacga gaaacataaa gataatagaa tggatgaaaa cagaaatatt
    16981 aatgagtgta agtgatttgt ttaatttatt gtttaaaggg gtaaaaccat tagatgaagc
    17041 aattcaagat actttagcaa atataataat gataacatat ctcttagcaa agagattagg
    17101 aattagtttt agagatgtgg attataaagt caaggaaaaa ataaaaattg gtatagatga
    17161 ggaccatagc gttgaaagat ggtatggaga tttatctaat ttaaaaaaac atatggataa
    17221 tagggagtga tattaaattg aataataaaa ttagactatc taaggcaatg tctataatag
    17281 ttttagctat aataatcgct ttagtaatag cctatattcc aatgctagga atatttagta
    17341 tgttagcagc tgttccatat gtaattatag gtgctattac tgatagaaag tattctctta
    17401 tatctatttt ggttacattt tgtgtactga ttctatttgc agacatttct tatgcattaa
    17461 atatatgtat aatgtattct ttaccaggta tagtaatagg aaagatgcta aagagaagct
    17521 ttgaacaaga ggacagtaat aaattcgaac cgatatatgg tggaaccata atatttgtac
    17581 tttcaatgct tgtatatttt tttgtattaa agacattttt aaatgtgaat ctattagatg
    17641 aagtttctaa gatgatatca gaaattgtaa atattcaaaa aaatagtttt tctgcaactg
    17701 aacttaaagt gtttgataaa atgaagccag atgagattgt aagttatttt acaaatatgc
    17761 taccaatgat gttattttta caagggttgt tatcagcgtt tgtcacatat tatttgagtg
    17821 tattttttat aaaaaggatt acaaagatga atataagatt tcctaagttt gctgattttt
    17881 atttaccggg aaatgctata ctcacaactt tcttacttta tttattagtc ttatttattc
    17941 aaattattgg ttcaaaatta tatacagaat taataatgac taatttacag ctagttttta
    18001 atttaatgtt tgtaattcaa ggtatagcag tttgcattta tttcttaaag aaatggataa
    18061 gacagggacc aaataaaatt atgttttttt cagggataat tttatgttta tttggattta
    18121 tgggaatatc ttttgttggt atggtagata gtataattga ctttagaaag gtaagaagct
    18181 ataaatccac ttaggaggat agaaatgagt aataaacaaa cctttaaatt aaatatgcca
    18241 gaaataaatt tatatataat tgttattggt atttctagta taattttgct ttattacaac
    18301 ttgtatgtag ggtgtttatt tttctgtatt tttgtatata tggtttttca caattggaga
    18361 actactaata ttagacgtca tgaatggaca gagtatattc aaaatttatc tttagatata
    18421 gatgaaacaa ctaaaaaagc cataataaat cttccaatac cattatgtat tttagaattt
    18481 gatggaaata tatcctggta taatggaaag ttttatgata tgattggtca aaaagattta
    18541 cttgataaaa atatagagga tatagttaaa aatcttaatt taagaaaagt attaaatgaa
    18601 aataaagaga tgtacacaga aataaactat aaagaaaaag aatatacaat aatttataat
    18661 gttataaaaa atgatcaaga aaaaaatcct aaatatttga tgatattata ctggatagac
    18721 aagactgaat atttaaaagt aaaacaaaat tatgatgatg aaaaaaatgc aatgatgcta
    18781 atacaagtag atggatatga tgaagtatta aaaagtgcag cagaagataa gagagcttta
    18841 attaatgttg aagtagagaa aatactttct gctttagaat taaattcaaa tggtgcattg
    18901 agaagaactt caaaagacaa attcttcttg gtaatgcata aaaaagagtt aaaaaaattg
    18961 gaagctgaga agttttctat tttagataca ataagacata ttgattatgg aaataatctt
    19021 cctgttacta taagtatagg aattggtata gatggagata cattaaatga gaacctaaaa
    19081 cttgctacgg gtgctctcga tttagcactt ggtagaggtg gtgaccaggc tgttgtaaaa
    19141 acaaaagata aatttgtgtt ttatggagga aaatctaaag ctgtagaaaa gaaaaccaaa
    19201 gtaaaatcca gattaatagg tcatgcttta agagaggtta tacaacaaag tgaccaagtt
    19261 tatataatgg gacataaata tcctgatatg gatgctatgg gagcagcagt tggtgtttat
    19321 gatatatgta aatcctgtaa caaaactgca aacatagttt tacaatctgt aaatgaatct
    19381 atagaaatat ttataaataa aataaatgaa aataattact ataaaaagtt gtttattgga
    19441 aaagaggaag ctattgacaa ctgcactaaa aatacattag ttgtagtagt tgatactcat
    19501 agaccaaatt atactgaatg tgaagaactg ttaaagctat cagagaaggt agtagttata
    19561 gaccatcata gaagaggtgt agagtttata aatgatgcag tgcttttatt ccatgagata
    19621 tatgtatcct caacgtgtga aatggttaca gagttagttc agtatatgga tgaagatgta
    19681 acaataaata aacttacagc tgaagggctt ttagcaggta taagtctaga tactaaaaac
    19741 tttgcattca aaacaggtgt tagaactttt gaagctgctt cttaccttag aaaagttgga
    19801 gcagatacta tagaggtaaa gaaattcttc aattctgatg taaaagattt cataataaaa
    19861 gctgaaataa ttcaaagtac taaaataatt aataatagaa tatgtttagc atattcaagt
    19921 actgagatag atagtataaa tgttataata gctcaaactg ctgatgagtt attaaatatc
    19981 aaagaagtag aagcttcctt tgtattaggt gaaaaagatg atactatatt tataagtgcg
    20041 aggtctttag gacaaataaa tgtacatgta cttatggaaa aattaggagg cgggggacac
    20101 atagatatag ctggagcaca gttaaaaaat gtttctctaa aagaagctta taagatggta
    20161 aataaaataa tagaggagta tttggaggag gaagaataga tgaaagttat attattaaaa
    20221 gacgtaaaag gaactggtaa aaagggagaa atgaaagaag taagtgatgg atatgcaaga
    20281 aatttcttat ttcctaaaaa gatggctgtg caagcagata gtgtagcaat aaaagaatta
    20341 aatgaaaaga ataaatctaa agaaataaaa gcacaaaaag agtatgaaga agcagtttta
    20401 ttaggtaagc aaatggaaga aattaatata gaaatatatt caaaagcagg agaaggtgga
    20461 agattatttg gttcaataac ttctaaagaa attgctgagc aattaaagaa acaaaaagac
    20521 atagatgtag ataaaagaaa aatattatta gatgaaccaa taagatcttt aggttcaact
    20581 ttcgtagaaa taaaaataca tcaaaaagta actactaaaa taagagtgga tgtaaaagaa
    20641 aaacagtaat aactagaggt gattaagatg gaagatatga cgagaattcc tcctcatagt
    20701 gtagaatcag aacaatcaat attgggttct atacttctag ataaagatgc tataataacg
    20761 gttacagaaa ctataaagcc tgatgatttc tataaagagg ctcacaaaat aatatatgaa
    20821 tgtatgataa ctttaagtaa taaaggtgag cctatagact tgataacatt aacagaagaa
    20881 ctaagaaaac aagggcatct taatgatatt ggaggaatca gttatattac aagtctttct
    20941 actatagtac cgacgacttc aaatgtaaaa tactatgctg atatagtaaa agaaaagtct
    21001 gtacttagaa agcttataaa agcttctaat gaaataataa atctaggata tagtggagca
    21061 actaagattg aagatgtttt agaacaagct gaaaagagta tatttgatat atcacaggag
    21121 aaaactagtg atgattttaa atctataaac ttagttttga tggatgccta tgatatgata
    21181 gaaaaattat acactaacaa gagtgatgtc actggtataa ctactggttt taaagattta
    21241 aacaaaaaaa taaatggtct tcaaaggact gatttaatat taattgcagc tagaccagca
    21301 atgggtaaaa ctgccttttc tttaaacttg gttcaaaatg cagccttaaa aggtgatgcc
    21361 tctgtagctg tatttagttt agagatgtct aaggaacaat tagtacaacg tatgttatct
    21421 tcacaatcta gtgtagaatt aaaaaaaata aagactggaa cacttaatga taatgattgg
    21481 cctagaatta tagatgctat ggcagtatta tcagatgcca aaatacacat agatgatact
    21541 cctggtataa aaatatcaga actaaggtca aagtgtagaa agttaaaaat agaaaaagga
    21601 ctagatttag tgcttattga ttatcttcaa cttatggaag gtgaaggtaa taatgaaagc
    21661 agacaacaag agatatctaa gatatcaagg tcactaaaaa tattagcaaa agaattaaac
    21721 tgtcctgtag tagcactatc tcagttatca cgtgcaccag aacaaagagc agaccataga
    21781 cctatgttat ctgatttaag agagtctgga gcaatagaac aggatgcaga tatagttatg
    21841 ttcttataca gggacgaata ttatcatgct gattctgaga gtaaaaatat aggagaagta
    21901 ataatagcaa aaaatagaca cggtgaaact ggttctgtgg aacttgtttg gcttggtgaa
    21961 gttcaaagat ttggggataa gttgagagac ctataatcta acgaatatcc acagaacgtc
    22021 ctttctttct acaggaaaaa tgtggataca tgtcactaaa tatgtccaaa cattgaaatt
    22081 tagctaaaaa actatccaca ctatccacag atggcctgtt gataagtgtg aataagtaga
    22141 taaagcaagc ttttggcttg ctttatctgt gtataaatat taattattag gaggaataat
    22201 atggaaaaaa taagcttaaa atatatatat cccaatataa ttaaagtact tgatgaaata
    22261 aacttattta gagtaattga caataattta agagaatcta ttgttgttta tgctaataat
    22321 gtggataatc agtatcatat aaacatgact aatacaaact ttggaaatat aattaacata
    22381 tgtaaattag aaaaactttt agatgtggat aaatttatgg aaaaagttat aaaatatgaa
    22441 aaagaaataa tagagaaaga agagttttca aaaatagaag aatatatgtt aaatattgga
    22501 gaatactaaa aaagttgaaa aaaatacgaa tgtttgatag aataagtaag taaagtgttc
    22561 gagagtatat aaaatataga ggtgaattac atgaaaacag ttgcaattgt tggttctcaa
    22621 tggggagatg aaggtaaagg taaagttata gattatcttg ctactcaagc agatgtagta
    22681 gtaagagggc aaggtggaaa taatgcagga catacattag ttgtagaagg taaaaaatac
    22741 gcattacatc taattccatc tggagtttta aatccaaata cagtgaatat aatcggaaat
    22801 ggaatagtgt ttgaccctaa aggattttta gaagaattgg aaatgtttaa aacggataat
    22861 ataagtacag aaaatataaa aataagtgat agagcacatg tcatattccc atatcataaa
    22921 gaactagatg cattgtcaga agaagccaga ggcgatttaa agataggtac aacaaaaaaa
    22981 ggtattggac cttgttatat ggataaaaca gagagatctg gtattagaat atgtgactta
    23041 atggataaag ataaatttgc tataaaatta aaagctcaaa tagatgctaa aaatgaaata
    23101 gttaaaaata tatatggaaa agaagaatta tttgattttg aaactatata taacgaatat
    23161 ctaggatatg cagagcaaat aagaaaatat gtggcagata cgtctgttat tgtatatgat
    23221 gcagtaagag caggtaaaaa agtattgttt gaaggagcac aagggacttt attagattta
    23281 gatttaggaa cttatccatt tgttacatct tctcatccaa catctggtgg atttgcaatt
    23341 ggtgcaggta tagggccaaa tatgataaaa gacgttgttg gtatagtaaa agcatatact
    23401 acaagagttg gagaaggacc gtttgttaca gagcaaataa atgaaactgg agataagata
    23461 agagaacaag gacatgaatt tggggtaact actggtagac caagaagatg tgggtggttt
    23521 gatgcagtta tagttaagta tgcagctaga gttaatggat tgactagtat ttcatttatg
    23581 ttacttgatg tattaactgg atttgataag ataaaagttt gtacgtctta taaaatgggc
    23641 gataagataa taacggattt cccagcttca ttagatgatt tagcaaaatg tgaacctgta
    23701 tatgaagaat tagatggatg gaatgaagat ataactcaaa tagataactt tgatgacctt
    23761 ccagaaaatg caaagaaata tgtagcaaaa atagaagaac tagttggagt aagtgtagat
    23821 atggtttctg ttggtccaaa tagagctcaa actataataa gaagaaatat atttgcttaa
    23881 tattttatca attaaataat ttaaaatagt tgaagaagag tatctcaaga taatgtttat
    23941 agttattaga gatgctcttt tatatttgac ttacattgat tttttattaa gttaaagcta
    24001 cttatacata ttctaactga aattaaagtt gaatttatgt agatacatat aggtttttta
    24061 aaaagaattt tgttgaaaat aaatggctaa agatgatttt aattatattc taaaaaaagt
    24121 aaaaaaaact attgactaaa aattatatag acgctataat ataacttgtg agtgaaagaa
    24181 acgacgaaaa aagtcgaaaa aatgtggtct attagctcag tcggtagagc acctgacttt
    24241 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaatatggc tcgttggtca
    24301 agaggttaag acaccgccct ttcacggcgg taacaggggt tcaattcccc tacgagtcac
    24361 cagtatggga ctatagctca gctgggagag cacctgcctt acaagcaggg ggtcacaggt
    24421 tcgagccctg ttagtcccac caaattttga attagagata aatactagat tatatgctgg
    24481 tgtggctcaa cggtagagca gctgacttgt aatcagcagg ttgtaggttc gattcctatc
    24541 accagctcca caaagtacat aaattcaaat taatataatt ttctatgtta caggatactt
    24601 ttaagtatct ttttttaatt tcatttttat tgtattaaaa atgaaattaa acatacaaca
    24661 aaattttgaa ttacttgcaa tgtgatagtt ttaaatataa aatttagaat cattctagca
    24721 taatattatg taatatagca aatttaattt atagtatcaa taaatataga ctactaagaa
    24781 tgtaaatgat ttgttagatt aaggtacatt agatatgtaa atagaaaaat aaaaagtatg
    24841 ttttaattta tgtgatataa gttttaaaca gagaagcaag tttgctctta cctctctatt
    24901 taaaaataaa aaaatgtttt ttaagggcta ttttatttaa atctttccca tctcaaatca
    24961 tctccagtaa attctaaaat cctaaactta tctaatattc tagagagagt tctttgagtg
    25021 taagtatttc ctatttgaga tggtgttaag ttggttgaaa taatgatttt tttaccagca
    25081 acaagtcttg tatttacaat attgaagatt tctccactag taaaagaatt attaagttca
    25141 gttccaaggt catctattat taataaatca caatcaaaca agtttttata attatcttca
    25201 cttatttgat tatttgtgtc tcttctaaat ttataatctt caagaatatc taaaattcta
    25261 aaagaagttt gatagattac aacattgcct ttatctaaca attcctttgc aatacaattg
    25321 cacatatagg tctttcctag tccagtagaa ccataaaaca gtagattttc actattatcc
    25381 ttcttaaaat catgaacaaa attttcgcag atacttaaat tatttagcat attttctcta
    25441 ggagatattt caccatcaga gcctttctta ggagaaaata tattcaagtt aaaattcgaa
    25501 aaattttcct gacttaaaat cctatctaaa ttagacattt tataagcttc atttacaatt
    25561 tcttgtttga ggcaagaaca tttttctcca ttaggtaaaa atcctttatc tttacaagaa
    25621 atacattgaa actttatttc caagtaatca agaggtatgt tactttcagc taaaagtctt
    25681 tctttcttta cttttaaaga ttctatattc tctttagtct tctttacaat ttcatcttta
    25741 gacttaggat ttaaaagtac tatcttagct aaactgagac ctattttaga aatttcatca
    25801 tctatagatt ttatctcagg gattctatta tatacctcat tttttctatg ttctaataag
    25861 agttcattat catctcttct cttagcatat ttagcaagta tttttcttat tttatcctca
    25921 ttcatttaat cacctactta aacttttctt tttgactttt tttaataatc tcatcgagct
    25981 catcagaggt atactgagtg aatgtttcgt tgaaattatg aaacttagtt tttttgtaag
    26041 tattgttgtt ttgagtagta ttaatctgct tcttattaat attctcctta actatacgtt
    26101 cttcctcttt ttgctttaaa tcatttaaat tttttatatt tttttcattc caatttttaa
    26161 ttataccatt tatataagat atacttgggt ttgaaatatt cttagactta gaacatgcat
    26221 ttattatcaa atctatatcc atattaaatt tatcaaacca ggtgtccata agttcttttt
    26281 ctgattttga tggttgtctt gaaaatccca attcattgaa aatcgtttta tataaaatat
    26341 atcgttcact tcttaccaaa aagctttctt caacatcttt tggcgtatat aaatttgaat
    26401 cataccaatt tctaataatg ccttcaatgt atttaactgg ctttgatgta ccagttttat
    26461 ctttgacgta ttcatatgcg caaagtatca tatcggggct catattgtat ttattcatta
    26521 tatccataat actcatcttt tcacttgggt cgagatatcg cccaactatt ttatttatag
    26581 aattaaacat ttttctaata cttggatttt cagaagtaga tacaacttta tctgtattag
    26641 attttataga tgaattattg cttaatatat tctcaatata gaaatttttt aaatctaaaa
    26701 attctattga ataattaaaa ttatcatatt cttcattgtc atgtattttt ataatcttct
    26761 tttcttccca gaatttccaa gcagataaca catcagacaa tggtatattt aggtttttgg
    26821 ctattgaatt attgtcgaac ttaggattag aagtaatatc acatgcttgt ctatatccta
    26881 aaagataaac ttttacgtat aacccgtccg ccattggcat aaatatatct ataaagatat
    26941 tgggaatagt gatttcacct aaatctattt cattgctttc tttaaaaaac attttatcac
    27001 ctcagattaa attatatcat tttttatatt atatttgtct gtaaacaaat aaaaggtcac
    27061 aagattagca tgtaaatatt ttctaaaagt attcattttg aaaaggagga gatattttgg
    27121 ttataaaatt caaccaaaaa gtaagaagaa cattatcagt agcgattcta tttatggtat
    27181 taataatagg ttgctttata gaaaaggatg acagtttaga aacatacaaa gaaaaaacta
    27241 attcaaaaat aaatcagtat aatatggatg ttatatttga tgatgagact aagaggttaa
    27301 tgtgcaatca gaatgttgac tacataaata atacaaaatc aaacatagat aaaatatatt
    27361 tccatatata tccgaatgct ttttccaaga aagattttgc tccttttgaa aaaagtgaaa
    27421 tgacaagggc ttatcctaat ggatttaacg aaggatatat agatataaag aatatattaa
    27481 ataataatag taaaatggag tataaaatta aaggagacaa aaatgacata ttagaaatag
    27541 aattagggaa agagttaaaa ccaaatgaaa gaatgtcatt agatataaaa tataacgtaa
    27601 aaataccaaa ttcaattgga agatttggat atggagaaaa tactataaat gttactaatt
    27661 ggtttcctat agcttgtgta aatgatgaaa ggggatggaa tttaaagagt tatgaaacta
    27721 ttggcgaccc attttatagt gagactagcg attttcatgt gaaactattg attccaaata
    27781 aatataaaat tgctcataca ggaaaactta ttgatgataa acatgataat aagaaaatgc
    27841 tatatgaaat agaagctgaa aacgttagag attttgcatt tatactaagt agtaaatttg
    27901 atgtaaacaa agtagattca aagggagtag ctatcaatac atataattta aataaaaagc
    27961 tttctaaaaa agcaactgaa gtggcaaaag attcaataaa tatatttagt gaattatttg
    28021 gaaagtatcc ttacaaagaa ttttctgttg tagcaagtga cttttttata ggggggatgg
    28081 aataccctat gcttgttatg atagaccaaa gtttatataa tgaaaaaaat gaatttttac
    28141 tagaatatgt tattgcccat gagacagcac atcaatggtg gtattctgct gttggaaatg
    28201 atgaaataag tgaaccctgg ttagatgaag cacttactga atattctaca atagtttatt
    28261 ttgaacaaaa gtatggtaaa gatatggcaa acaagcttat aaaaactatg gagatacaga
    28321 caaaaagtta tttgagtgaa aatatattta agccagcaaa tgagtataaa aattctactg
    28381 aatacagctt gaatgtttat acaaaaggcg caatagcatt taatgaaata agaaaagaag
    28441 ttggagataa agtattcttt gagactttaa atgagtacta taataaatat aagcataaaa
    28501 atgctaatgg aagagctttt gtggaactat ggaatagtaa aggtgtagat ataaataaga
    28561 ttataagtga atgtaagtaa atagtgaatg taagtaaatt ttgtgcagag tattaaattt
    28621 tatttaatac tctgtttttt tggtataata taatttataa ggcagaaaaa gaggtgaagg
    28681 aatgctaaag tattgttcag tagggagtgg tagtagtggt aattgccact atatagggta
    28741 taaaaatact aatatactag tagatgcagg attaagtggc aagagaataa ctacaggtct
    28801 taaagacatt ggtgtagatg cagataaatt aaaaggaata tttataacac atgagcatgt
    28861 tgaccatata aaaggagcag gtatattatc aagaaagttt gatataccag tatttgctaa
    28921 tataaaaaca tggtgttcta tgaaggacaa attaggtgac atcaaagata aaaatatgaa
    28981 agtatttgaa aatgataaaa cctactcatt aggtgatatc atagtaagac ctttttcaat
    29041 agaacatgat gcgtctgacc cagttggata taatttttat acagagagtg atgaaaaaat
    29101 gtctatagct actgatatag gatgtataac tcaaaatata aaaaaacatc tatataaatc
    29161 taaattagta gtattggaat caaattatga ccctaatatg cttatgatgg gttcatatac
    29221 atatgcttta aagaaaagag taatgtcaag tactggtcat ttatccaatg aagatgcagc
    29281 taatttctgc gttgaactta ttaatgaagg tacagaatct atattattag cacatttaag
    29341 caaagaaaat aattttcctg agttagcgta tgagacttca aaaggagtat tagcatcaaa
    29401 tgatatagta gttggacaag atgtaaaact agatgtacta tcacgaaatg atgtttctaa
    29461 tgtttatgag atgaagtaga ggtgaataac tttgctaaag agaaattttg aaaatgttga
    29521 ggataataaa attttatact ctataaagca gggtatgatt ttagcgatac ctgctattat
    29581 gactggttca actgctcttg taattttgaa tcttccaata aaggcttatc aagaatattt
    29641 atcaggcttg tttaatggag aggttacaaa tattcttaac tttataaatg attctacttt
    29701 aggaattatt tcattaataa tattattaac aataagctat agttatggaa aaatatatgg
    29761 gagcaagtat acagtacttg ttcctatagt tgcaatgtgc tcttttttag tattttcaca
    29821 tggtaatgag cttaattctt atatagaaat atttaaaaca aagtggttat ttacatctat
    29881 aattgtttct atgacatctt ctgtattgtt tgtaaaactt acagaatctt ttgttacaag
    29941 tgtaaagttt catacagaag gggctgatgc agactttaat atggtagttt cggctatagt
    30001 tcctttcata atagtagtat ttttgttttc tatttttaga gtggtaatga taagtctaat
    30061 tggcagttca aactttcaag atatatttta taatttattt tcaaatgttt ttaataaaat
    30121 gggaacaaac cttatgagtg ctctgttgtt tattttttta atgcacttta tgtggttttt
    30181 tgggattcat ggtagtaatg tattagatac cgtcgcaaaa aatttatttg aaaatagtat
    30241 ggcaattaac ataaatttag ttaataataa tcaattacca acagaaattt ttactaagac
    30301 cttttttgat acaatggttt tattgggagg atgtggctct ttactatgtt tagtaattgc
    30361 aatttttctt agtgaaaaac gtataaatgt aagaaagtta gctaaaattg cttcaatacc
    30421 agctcttttt aatatcaatg agatgctagt tttcgggata ccaattgttt ttaactatat
    30481 tatgtttgta ccttttgtaa ttactcctat aatcttaacc gttacaagtt atgtggctat
    30541 gtctacaggt attgtaccat gtacagtttc agctgtagaa tggacctcac ctatattttt
    30601 aagtggatat atgtctacag gttcaataag aggtagtttg ttgcaagtat ttaatttatg
    30661 tgttggtgtt atgatttata ttccgtttat aaaaatgtcg caaaatagat atacatatat
    30721 gtttaaaaat aatatagaaa atttaacaaa cttattaaaa aggtatgaat taacaggtga
    30781 acagcctaat cttctaaacc ataatggtaa aataggttca atttcaaaaa tgttggcatc
    30841 agacttgaag tttgctatga aaagaggaga aatagagctt ttttatcaac cacaagtcaa
    30901 ttataatgga aatgttgtgg gagcagaggg attacttaga tggaaacatt caataggagg
    30961 gttcatttat ccaccactag ttataatttt ggctaaagaa gaagggttct tagatgagct
    31021 tggagagtat attattaata aagcatgtat tgatttaaag aagagtgaaa agctattgag
    31081 aaatcctgtt aagttctctg taaatatatc tccagaccaa cttgatgacc ctaagttacc
    31141 agagcaaata aaaaatatca ttagtagtaa tgatataaat cctaatatgt taggtataga
    31201 aataacagag caaattgcat tatcaggttc acaaataatt attgaacgta tcaatgcgat
    31261 acataacttg ggaataaagt taattatgga tgattttggg atggggcata gctctcttat
    31321 atatcttcaa aataataact ttgatatagt taaattggat ggttcattag ttaaagagat
    31381 acttacaaat aaaagaagta gtgaaattat aatgactatt gtaaatcttt caaaaaattt
    31441 agattttgat atagttgttg agtatgttga aaatatagac caaagggata agttatatgg
    31501 tttaggatgt gaaatatatc aaggatatta ttatagcaaa gcaatacctt ttgaagagtt
    31561 tgtagaatat gcaaatcaag aaagagagat ataattttat aagagcttgt aaattctagt
    31621 aatgcataga tttgtaataa aattaagttt aggagaaata tatgaatata agtatagtag
    31681 tagtaggtaa aattaaagaa aaatatttaa aattaggtat tgatgagttt aagaaaaggc
    31741 tttctaagta ttgtaagtta gaaattatag agttggatga tgaaaaagct ccagagaatt
    31801 tgagtgttaa ggaaatggaa attataaaag acaaagaagg aaagaaaatt ttagggaaga
    31861 taaagcataa tagttatgtt atagccttgg ctattgatgg taagggttta tcctctgaag
    31921 agcttgctaa gactatgagt gatttagcag ttagaggtaa tagtagtctc tgttttataa
    31981 taggaggttc tttagggctt tctgatgagg ttttaggaag ggctgattat aggttatcct
    32041 tttctaagat gacttttcct catcagatga tgagattgat attgttagaa cagatttata
    32101 gggcttatag gataaataat ggagaaccgt atcataaata aatgatacgg tttttgttaa
    32161 tttgacactt aataatatta aaataacaaa aaagtattga aagtatattg catttaaggt
    32221 agaattaaat aaaatattat aacattataa tgtttatatg gttgttaata aaaatttgga
    32281 ggaaaatata tgataaaaat aatgttggcc tgttcagcag gaatgtctac aagtttatta
    32341 gttactaaaa tggaaagtgc agctaaagaa aatggtatag agtcgcaaat ttgggcaata
    32401 ccagaatcaa ctatacaaaa tgaaatagaa aaatgtgatg tattattact aggaccacaa
    32461 gttagatatg tattacctaa ggctcaagaa attgctaagc catataatat accagtagaa
    32521 gttataaata tgatgcacta tggaacagtt aatggagaag ctgttttaaa tagagcgata
    32581 gaacttaaca acactaagta atataaatta taatgtattt ttaatatata aattgggaga
    32641 atcaaaggat ggatgaaaag actatagaaa tatcttttaa tataataggt tatgcaggtg
    32701 aaggtaaagg attagctttt gaagcaataa aagaggctaa gaatggaaat atagaaaaag
    32761 caaaagaatt attaaaagag tctagggaag ttataaataa agcacataga tatcaaacag
    32821 aattaataca aaacgaagct agtggaaaca ggactgaaat aagtgtcata cttatacatg
    32881 cacaggacca cttaatgaat ggcatgaatt ttcaacagtt agctgaggaa atcgttgatt
    32941 tacatttgaa attacaagat aaataaaaat taaagataat aaattaagag agttcttaaa
    33001 agatacatta tgtgtagcac actaattgtt agattattga ttcaatattt agtgagtaat
    33061 acattaggat tattttgagg actcttttta tatttagttt aaagttaata aatatttatt
    33121 attaataata taagattata tgttataata ttataacata taatttaaga ggtgaatcat
    33181 gttaagaaat caaccattat atatacaagt tgtagatata ttaaagcaaa aaatattaaa
    33241 taacgaatat aaaattgatg aaaatattcc aacagaaagg gagttagagc aagagtttgg
    33301 agtcagtaaa attactatta gaaaagctat tgagatatta gaatcagaag gatatgtaga
    33361 gaaaaaaagt ggaaaaggga caaaagtaat aagtaatagc ctttttaata agctatcaaa
    33421 agcagagtct ttttcaagta tacttttatc taaaggtcat aagttatcta aagagactat
    33481 ttcaattgaa aaaataataa atgatacaaa ttcacaagta tttagtatat ttggagaaaa
    33541 atgtagcaag attactagaa tgtattattt agatagtaag ccatatatat tttttactca
    33601 ttatattcct tttgatgaaa atctaactgt tgaaagtttt aataatgaaa gttctttata
    33661 tatgtactta tataggagaa atataaatat aactagattt gaagatgaat tttttataga
    33721 agaatcagaa ctagaggtat caaaggcttt aaatctagct aaagacatat tattaggaag
    33781 aaaaagaaag acctatgatg agaatgataa tatagttgag ctatcatttg caagatataa
    33841 tacagaagta caaaactatg taattaaata cgaaatataa taagaatcct gagtttattg
    33901 aaatgataat atgctcagga tttttgttta gttgtaaaaa taaaaaatat tataacatta
    33961 taatgtttac aaccaaacat tataatgtta taatattttt aataaacaag tgatattatc
    34021 aattacataa aaattagttt atgttatgaa atcgttattg cattataaat aaaggataat
    34081 atcaataata cagggggata actatggaaa aattcgcaag ttttttagag cgcaagatta
    34141 tgcctgtagc agcgaagata agtaatcaga gacatatgag agctgtaaga gcaggtataa
    34201 tagcaacatt accactaaca atagtggggt catttttcac aatattatta aacataccta
    34261 ttgatgctta tatggatttt atagctccat ttaaagcaat attagatata ccatttagat
    34321 atactgtagg atttttatct ctatatgcta cttttgggat agcatcatca cttgctaaaa
    34381 gctacgattt agatacaaca ggtgttggta tgcttgcagt tatggcattt ttagtttcta
    34441 caatagttcc aactcaagta gttgaaccag taggtggagt tatagaagct ggtagatgga
    34501 tggatatggc aaaacttaat tcgccatcat tatttggagc tatcgtaaca tcattaatta
    34561 cagttgaaat atatcgtttt atgaaggaaa agaatattac aattaagatg ccagcaggag
    34621 ttccagtaga ggtttcaaat tcatttactg cattattacc gacagcagta attttaatgt
    34681 tattctgggt aataagacat attctaggat ttgatataag tgttgcacta agcgctatgt
    34741 tatcaccttt aaaaggattc ttatcaggaa atagtttagg tggtgggtta ctgacagtat
    34801 tcctaataac tttattctgg gtacttggaa tacatggtcc agctataatg ggaccagtaa
    34861 taagacctat atgggacatg tctatagcag aaaatataac tgcattcaca gaaggagtat
    34921 cagctcatgc aatgccaaac atatttacag aacaattttt acagtggtat gtatggatag
    34981 ggggagcagg ggctactctt gcattagttg taatgtttat gttttctaag tcacaatatc
    35041 taaaaagttt aggtagatta ggatttttac cagggttatt caacataaat gaacctataa
    35101 tatttggagc accaatagtt atgaatccaa tactagggat accatttata attggaccta
    35161 taataatgac aattctatca tatgttctta ctattacagg ggttatacct atgatggtag
    35221 caagattacc atttgcaatg ccagcaccaa tagcagcagt aatgagtact aactggagta
    35281 tactagcagg tatcttagta ttaatcaact ttgtgatatc atttgctgtt tattatccat
    35341 tcttcaaagt ttttgaaaaa caacaattac aaagagaaca agaagaaatg agtgaaaatt
    35401 tataaaaata catagatata gaggttttgt actatataaa atatagaaaa agcttataag
    35461 ttattaaggg tgaaggtagg aggaatgacc tttgccttta attttatata ttttaaataa
    35521 tgtaatttga ggaggagaaa gatggaaaga agattaggaa tatcagtata tccagagcat
    35581 agtaacatgg aagatgataa ggcatatata aaaatggctt caaaatacgg atttaaaaga
    35641 atatttatgt gcatgctttc aattacaaga cctaaggaag aagttaaaaa acattttaaa
    35701 gagattataa cctatgcaag agaattagga tttgaagtaa tccttgatat agctccaaac
    35761 atatttgata tattacaaat atcttatgat gatttaacat tttttagtga gttaaatgct
    35821 agtggaataa ggcttgacta tggatatgat ggaagtaaag aagctatgat ttctttcaat
    35881 ccatatgatt taaaagtgga actaaatatg agtaatgatg tagcatactt ggataatata
    35941 attacttaca agccaaatgt aaataatata tatggttgtc acaattttta tcctcaaagg
    36001 ggtacaggct tgccatatga tttttttgta agatgttcaa atagatttaa aaagtatgga
    36061 ataagaactg ctgcttttgt taattcaaaa tacgcaacaa tagggccttg gaatataaat
    36121 gatgggctgt gtacgcttga aatgcataga aacttagata ttgctacagc aaccaagcac
    36181 ttattcgcaa caggattgat tgatgatgta attgtaggga atgcatatgc aagtgaagat
    36241 gaattaaaaa gaatgtctga agtaaataaa tatcaagttg tatttaatgt agtaactgaa
    36301 gaaaaaactt ctgaagtaga aaagacaata atggcaaatg agcaacactt tagaagaggg
    36361 gatataacca accaagtagc aaggtctaca gaagtaagaa aaaaatatgc taaagaaaat
    36421 ttccctaagc atgataatac gaccatcttt aaaagaggag atgtagtgat tggtaatgat
    36481 gactttggaa agtataaagg ggaattacag ttgatacttc aagattgtgc agatgaaaga
    36541 aaaaataagg tgggtagaat aaaggaagac gaaataatac ttttagactt tattgaacct
    36601 tggagtaaat ttaaacttat tttagaggga gagtaaagtg aagatagaca tattaataaa
    36661 atgtggaaaa acagtagata aaagtataat tgatatagct atattagacg ataaaataat
    36721 agaagtaaaa aatcagatag atgattctaa gtatcaggca aatcaaatta taaacctaga
    36781 tggagaaaaa tttattagtg ctggttggat agatatacat acgcactgct acgaaagact
    36841 agacctttat aaggattatc cagatgaggt tggaataaaa agcggtgtta ctactgtagt
    36901 agatgcagga acaacaggag ctttagatat aggtaaattt tatgaagata ctaaaaaata
    36961 taaaacaaat gtatatgctt taataaatat tgctaagcag ggaataacat ctcaagatga
    37021 actttcaagt atgatgaata ttgatgaata tgaacttaga agagcagtta aaaaatacaa
    37081 agattttgtt gttggaatca aagctagaat gagtaaaagt gttgtaattt caaatgatgt
    37141 tgaaccttta aaagtagcta aaagaataaa aaatgaactt aatctaccta tgatggttca
    37201 ctttgggagt tctcctccaa cgatagagga tatatttgac tatatggaaa aaggagatat
    37261 attaacccat atatataatg gaaagccaaa tggaatactt agaggcaatg aggttaaaaa
    37321 agaaatcata gaagctagag aaagaggtat aatccttgat gtaggtcatg gaaccgaaag
    37381 ttttagtatg gatattgcaa tgaaatctaa ggatgcagga atttttccag acactataag
    37441 tacagatata tatattaaaa atagaataaa tggtccagtg tataatctat ctacaacaat
    37501 ggaaaagttt atatatatgg gctattcact tgaagatata atagataaag ttactaaaaa
    37561 tgcagcagat gcaataagtt taaaaaataa aggtttaata aaagaaggat atgatgctga
    37621 tttaactata tttgatgtgg ttaatgaaga aaaagaactt caagattctt tgaataagtc
    37681 agtaatcaca agtacgtcta taaagccaag agcagtagta gtcaatggag aatatttaaa
    37741 tataggtaag tctataggag agaatgagta gaagggagaa actattgtat gagtagtata
    37801 tttgaaaaat actctgtaag acgtgttata aatgcttcag gcaaaatgac tatactagga
    37861 gtatcaacag tagataagga agttattgaa gcaataggtg aagggtgtaa aaatttcttt
    37921 ataatagaag aacttataaa taaaacaggt gaatatatag caaatttatt agggtgtgaa
    37981 agtgcactaa tagtttctag tgcatctgct gggattgcac agagtgtggg agcaatgata
    38041 gcaaaagatg atatgagtct agtttacaat gtaaataatc ctaagaaaca agttaaaagg
    38101 gaaataataa ttccaaaggg acataatgtt gattatggag ttccagtaga agtaatgata
    38161 agacttggag gaggagaagt tgttgaggcc ggttatgcta atatgtgtaa caaggaacat
    38221 ataataagag agataaatga aaatacagca gcaattatgt atattaaatc tcatcattgt
    38281 gttcaaaaga gtatgctaag tgtattagat gcagtacaag ttgctaaaga atataattta
    38341 cctttgatag tagatgcagc agcagaagaa gatttaaatg tatattatga attgggagca
    38401 gacctagtga tatacagtgg tgctaaggct atagagggtc ctagctcagg gttagtaata
    38461 ggtaagaaaa aatacataga taatgtaaag cttcaatcaa agggtatagg aagagctatg
    38521 aaaataggaa aagaaaatat tgtaggtcta acatgtgcaa ttgagagata tataagtaat
    38581 gataaagtaa ctttaaaaga aatggaggat aagctaaatc catttataga caagataaat
    38641 actataaagg gagtgagtgc tagtataact agggattcag caggtagaga aatacttcgt
    38701 ggagaaattg actttaatga agatataata aataagtcaa cacaacaaat tataaatgaa
    38761 cttagaagtg gagagatagc tatatacact agagactata aggctaatga aggcaagatt
    38821 gagattgata taagaagtgt aactggaagt gatttagata ctatttataa taggataaaa
    38881 aatatagtag aaagttagag tgtagagtaa gtgacattat aatgatttaa taaattaaat
    38941 tttaccaatg gaggggataa atatgaattg tataccacaa tattataaaa atagagtttg
    39001 tttaaatgtt cttgcaggaa gcataaaaaa tgctaaagaa gtatatgatg cttgtgacgg
    39061 gcatgtattg ataggaatac tatcaaagaa ttatagcact gtagaagcag ctattgaaga
    39121 tatgaaaaaa tactcaaagg aaatagataa tgcaatatct gtggggcttg gagctggaga
    39181 cccaaatcag tcaaatatgg tatctcagat ttctaaagaa cttcagccac aacatataaa
    39241 tcaagtgttt acaggtgtgg ctacgtctag agcattgctt ggtcaaaatg atagtataat
    39301 aaatggatta gtttctccaa ctggtaaacc aggatttgta aaaatatcca caggtccatt
    39361 aagttcaaaa gaaaaagatg gtatagttcc tatagaaaca gcaatagcta tgatgaaaga
    39421 tatgggagga agctcaatta agttttttcc aatgggaggg cttaaaacaa aggatgaata
    39481 tatagaagtt gcaaaagctt gtgcaaagca taatttttat ttagaaccaa cagggggtat
    39541 agacttagat aacttcaaag agatagttca aattgcactt gatgctggtg tagaaaaagt
    39601 tatacctcat gtttatacat caataattga taaagaaaca ggtgaaacga aagtggaaga
    39661 tataggagta ctttataaaa taatgaaaga attattggga tagatttatg aatatattag
    39721 cttttggtga aataatgatg agactttcag tacctgacta caagttctta acacaaacca
    39781 atgaactaaa ttatataact acaggcacgg gattaaatat attatctgga cttaaaaact
    39841 ttggatacaa tacctatatg ttgacaaagt tgcctaataa taatgtaggt aaagcatcaa
    39901 gtgcaaatat aagaaagctt ggagtaaaag atgactttat cacgtatgga gggaatcata
    39961 taggagtgta ttttcttgaa aatgggtatg gagaaagacc ttctgaagtg acttatttga
    40021 ataggttaaa tagttcattt tgtgagagta aattacaaga ttatgatatt gataaatgtt
    40081 tagaagaaat ggatatagtc catatttgtg gtattgctat gcaacttaca gattcagtta
    40141 aagagattgc tttagaacta gctagaaaat cttatgaaaa ggggataaaa gtattctttg
    40201 attttaattt tagacaatca ttaaatccag aaaggactta tgatgattta gtaaatgatt
    40261 ataagaaaat actaccatat tgtcatgggc tgtttgctag ttatagagat ataaataaga
    40321 tacttaatat agatagtcta aatataaaat ctagttcaga attgattaaa aaagagtatg
    40381 atatagatat tgtagctgga actatgagaa gatttgatga tatgaataat agatttataa
    40441 agggatatat atcaaatgat gatggttatt ttgaaagtag ggagtataag ctagaaatat
    40501 atgacagggt tggtgccgga gatggatatg tagcaggaat tatatattca tatttggaaa
    40561 atatgaagag tgaagatata gttgagtttg gggttgctag ttcggtgtta gctcatacaa
    40621 cttatgggga taattcttta gtaagcacag gtcatgtact tagactgatg aataatgaaa
    40681 aaatagatat attgagataa tacatttgta tataataaaa tttttatctt ttttagacac
    40741 atttttgact tgcttatatt tgttaaacag agttattttt aatgattata aatattaaat
    40801 taaaagttaa ataataaaat agtagaaaat agcattaact atagaaaaaa tggttaatgc
    40861 tatttttatc gttaatattg attaatttta tattatagaa atgaactttt ctaatgctgg
    40921 atttgtattt tttttattcc atacaactac aacttcaatt ctattttcta cttcttttat
    40981 aggtactatt gcaatatcac gttctatcat tgtatctgta acacaaaatg aaggtaaaat
    41041 ggatattccc atgttagaag ataccaatga ttcaatagta ttaagattat ttgaacgttg
    41101 taccacatta ggtacaaacc ctgcttcacg atatctattc tgtatcattg acttttgtcc
    41161 atactcatcc cctgtttcct ttacaaaaat aaatttatca tgttgtaatt caattaaatc
    41221 gatagaatcc tcattgtaaa gtggatgacc aggaggtaaa agagctacca aagaatatct
    41281 ttctacagtt ttatattcaa aatcttttaa tggttgattt tctggattaa taacaaaagc
    41341 gaggtccata gaattatttt ttactaagtt taaaagttcg gttatttcat tagtacaaat
    41401 atccatagat atattagggt atgtattaga aaattctttt aaatattgtt gaagtttgtt
    41461 tttttcatat cctattaagg ttccaatgct caaagttcca aaaaaaccat gtgcaaattg
    41521 ttgtgttttt gcaatagcat cattgatatt ttttattata gtacgtgcct cagataaaaa
    41581 aacttttcca gctggtgtta gttcaacatg acgtttactt cttgtaaata gagttacatt
    41641 tatttgttct tctaaagctt ttatttgctg tgtaacagca gtttgagaaa tataaaattt
    41701 ttctgctgtt ttggtaaaac ttagattttc tgctacagat ataaaatatt ctaattgttt
    41761 agtattcaaa taatcacccc tatttaaact tgattgtttg taaaccttat ttatctataa
    41821 ttaaagctta ttatagtata agaaaattta cttttaaaga atattagaaa atatttgtat
    41881 aaaaatatat gttttaagta tatctttaca ttagatttta taaatttatt aataagaata
    41941 acttatatat tccaatgaaa acgatattgg atttaagtaa gtttgtctca taaaatgaaa
    42001 gtatatcaat tgataatact ttaaataaga agggagaaaa aatggaagat aaattttatg
    42061 caaaaggcaa cggaaataac ggatatatta aaaatcttga agtttgttcc tttaataact
    42121 tagatggaac ttgtggaatg tttcaaatgg ctctgtacaa aagagatgaa aaatactatt
    42181 tatatggatg ctgttttgga ggaaataaaa aaaatggagt aatgattagc gatattacag
    42241 acccttataa tccacaattt ataaaacatt ttcaaatgtt agaccctaaa gagtatccta
    42301 caacaacaac tcccaaaatt caaatagcag atgatttaat gatagtagca atgagttgtg
    42361 gaagtggacc aggagcactt gttgaccaag ctaaattagc aaatattaag tgtgaagcag
    42421 gaattagaat atatagttta aaagaagacc ctttaaatcc taagttttta ggatattggg
    42481 attgtggctt aaagcatgta atgggtgttc atagattcat gtacaatggt ggaagatatg
    42541 tacatttatc aagtgattgt gttggctttg aaggtctgat ttatagggtc atagatataa
    42601 taaatcctac taatccagtg gaaataggta aatggtggag accagaccaa tatgcaaatg
    42661 gatatccaaa tagaactttt gatgcaggag cacctcattg cccagaattt atggataaag
    42721 gatggcttca tggacctcca tttgtaagag acggaaaagc atattgtggt tatggaggag
    42781 ctggtttagt tgtattagat gttgaagatt taacaagacc aagatgctta ggtgaattgc
    42841 catttatgcc tgcattttct agtagacttg caggtgcaag aactcataca gcattaccat
    42901 tgccaggaag agatttagtc gttgttcaaa atgagggaga aagattccag ttctttaaac
    42961 cagataacat tacagatgtt caagctatga ataatataca tatggttgat gttagtgacc
    43021 caacaaaacc aacattaatt gctcaatttc catatcctga agttccaaaa gatttccctt
    43081 atcctaactt taatgttgcg ggattaggaa aaccagggcc atttggccca cataatcttc
    43141 atgaaccaat ggataataag ccatggttag agcaaagagg agatagagta tattgctgtt
    43201 atttccatgc agggctaagg gtttatgatg tatcagaccc atattatatc aaagagctag
    43261 catattttat accaccaaat ccaaataaaa caccagaaga atcttatttc ccaggattcc
    43321 caggaccacg cttggcagta acagaagatc ttatcgttga tgatagaggc tacatcatca
    43381 tagatgcttt agatgatgga ttctatatat taaaaatgaa agaagattaa ataaatacta
    43441 aagagggaga gttgaaaatg aaaatactag gtatttcttt tggaacaaaa aatggaaata
    43501 atgacacaat gtgtaaagtt gcacttaaag gtgccaaaga agcaggggct gaagtagaat
    43561 ttattcaaat gtcatctatt aacattaaac attgtactgg atgttgtgcg tgtgttaaaa
    43621 ctttattatc tgggaaaggt agtatgtgtg tattaaaaga tgattttgaa tggttgcttg
    43681 acaaaatgaa agatgcagat ggtatagtag tttcagaccc tatttttgaa gaaggggcat
    43741 caggattatt ccatactata atggaccgtt ttggacccag agcagataga ggaaataaca
    43801 ttataggaac aaaagttgca gaagctatag gaggaaaaat tccagattca agaatgctta
    43861 atgataaagt aatatcattt atgggaatag gaggttctga ctggggtaca agagttcaat
    43921 gtgaacatgc tatgctagct ctcataccaa tgtggaaagt tatagataat gcttggttcc
    43981 catgggcaaa ggaattagta atggatgata caaggcttgt tcaagttcat caaattggtt
    44041 taaatatagt tgaagcagca aaagattttg aaaaagcttc atatcaagga gaagaaggtg
    44101 tatgtccaca ttgccacaat agattattct accttgagcc aggtactaaa aaggcaattt
    44161 gtgctctatg tggtatagtt ggagagttag atactataag tggaaaaact atatttagtt
    44221 ttccagaaga gcaattaggt catgctcatg atactttacc aggtaagttt atacatggtg
    44281 atgatattaa gaaaatggaa ggtcattatg cagaagttcg taaaacacaa gaatttaaag
    44341 aaagaaaaac atcttataat tttatagagc cattaactaa agaaaagcaa gctggagttt
    44401 cattaggata ataattaaaa tactaagact cttttttaag gtatgttaga tgttatagga
    44461 ggcttattgt atgaatgaaa agtcaagttt taaacaaatt gaaattattt tagcagttat
    44521 ttttttggta cttacatatt acaaggttag attaactcca gcatttattg ttatatttat
    44581 accactatat ttaagttatt ttgcagtatc tatgattgta aatcaaatag caattaagct
    44641 attggagata aggcaagcta gggaagctac taattacaat ataggaaatg gatagaaaga
    44701 aaaaagaaat attaacactg tctatgggga ttgctttact acctccttta tgggcagtat
    44761 tagcacctta tataggaata aaaactggag ctgtagcact aatctgtgca ggattatatg
    44821 taactaatgg taataaacaa aaagatggat taaaaataat gtttggtttt tggtgtggag
    44881 atttatgggc agtgttagcc attttaatta tgggatatat gaatttcaat caaaacctag
    44941 aattattttt aacacttagt atattaggtt tttttgcagt tgtaattgca tccttatttg
    45001 aaaaaataat tttcctacca tcatggcttt gtggttgggc tataggtcta actattatga
    45061 caggtgaaaa tattattaat ttacaagata tttgtattca aattgctgta gctatggcag
    45121 taggagtatg gtacgtgggt gcaggtgtag atttgtttac aatatatatt ttgagtaaag
    45181 ataggaaaaa gggaaattga aagcatgtga atatactttg attataggag aatggctatg
    45241 ggttttgaat ttaaaataat gagaagttta atatatgtag gacttgccaa ggaagaatat
    45301 agacctaagc taatggactg gttatatcgt caccatattc cagatagtat tagcactttt
    45361 ggaccatatt gtactaaata tgccttttat caagcatatc ctacaccaaa tgaaggtgag
    45421 cgttttggtg cacgtaagat gcaactaaca gaacattatt ggcttgtaga tgaacatatg
    45481 cctgagatgg caaatagaat tatgacagaa tatatgccta tggatgttct acgttggcaa
    45541 gggtgtatac cagatgtaga aaataaaagg gttcatgaaa atgcagaaag tggagatgca
    45601 ggacgtgcag taggtggaga taatggatgt ccaccattta tatttgcctt tgttccaata
    45661 aactgggaag aagactttag aggaaaagga cgtactgtac aagatggacc aaactatcgt
    45721 tggcaattta tgattaagta tccagatggt atctctaaag aagaaggaga aaaatggttc
    45781 tatgatgagg tagtgccata ctttacaaac tgttgctatg ttaatcgttt tgtcagtagt
    45841 aaaataatga ttaattatgg agcaactgct tttgaccgtg tatcagaact atggtttgaa
    45901 ggggaagaag aatggtataa agctgtggtt gaagagacaa agtcgtttat taaaaaacca
    45961 gaatgggcac aagaagagga gttcccatat ttaaaaccac aattcaatat cgcatcagta
    46021 ttcttaggtg atatagcaac tatggatgca tactcacagt atcgtggata tataccaatg
    46081 agataaaaat tatagtttgt gataagtaga ggaggcattt actcttactg gtagaagaag
    46141 acttattgta tccttaaaag taggtgaata tatagtttta tatttaaatt tatataaaac
    46201 taagactaat aaatatgaaa caatagaatt agaataattt gataatattt gttaaaagag
    46261 catttagaat gtgtttaaag attaagatat tccttttatg gtagcagata gttgaaataa
    46321 taaaactgtt atcatgaaag gaatattttt atgtatagga aatttttaaa aataaaagtt
    46381 aaatatgttt aagaacatct taaagtgaaa gaatcattgt tagaaatatg tagacaatat
    46441 agtctttata acgtatcata ttaacaagtt tacacttgga ttatgaaata aaaagagtaa
    46501 ttccaaaggt tgtaaatact atgctgactt tgatacttca aagtttaggt gattttgtaa
    46561 ttgttaatag aaatacgttt aatgagcata aagaatttat ctaataatag gagaatataa
    46621 tgtatacaat tggacaagta tctaaatttt taggaatatc tagagataca ctaaaatttt
    46681 atgaggacaa aggattagta aatcctaaga aaaatgatga aaatggatat agaatatata
    46741 atcaggttga tatctatgat attgcaacaa taaactttta tagagaaatt gatatagaaa
    46801 ttaaaaagat tcaagaaata agaaaaagta agagtataaa caatttagaa ctcttactag
    46861 aagagaagga acaaatcata ctaaaagaga tagaatataa aaagcttctc ttaaaaaagg
    46921 ttcagttagt caaagaagat tgtgacaaaa taaaaacata ttttggaaaa tacacaataa
    46981 aagaaatgaa gcctttggaa gtaaaaggta aaataaaaca tgttactaac tatgaggaat
    47041 atgaagcctt aaaagagaat acaaacaact taaaaaaagc agttacatta actgatttaa
    47101 gaagagtaat aaattttaat gaagaaggta taatagaaga aaaatttata attgtaaggg
    47161 aagtagatga ctacaacaaa atcatagaag gtgaagttct atctcatccc aattgtattt
    47221 atactattat tgaagatggc agatggtcaa ctggaggaga aaatattgat aataaagtgg
    47281 aagacagatt aagagaaata gcaatagaaa atgaatgtga attacttgga atagtctatg
    47341 ttaatatatt gcttactact tatgaagaag ggattgagcg tatattttta gagatttatg
    47401 cacctataaa ataatataaa agcattgacc tgggacttac tcataggttt aaactcatga
    47461 tataagcagt tataacttat atagcaatta aatattaatt tgaaatgagg attacttatg
    47521 agaacagaga aatcattaaa aaggcctata gccagtttta ttattctaac aaatataatt
    47581 tttttaccac tttttttatt ggtagtagtg actataatgt taaaacttcc aacagtgata
    47641 tctgacattg cgttgtgtat atcagcgtgg tcatccactt ttgcatttat gatattattt
    47701 aaaaaaattt atccaggtaa gaaattttta gtacacgtaa aagatagatt tagaaataaa
    47761 cttaaatttt caacagtatc tataataatt agtatacaag tcctaatagc tgtagtagtt
    47821 atatttatat tagcatctaa aaatcatgga ataatgccta actttacagt ttcatcatgg
    47881 ggagcattta tatatttatt tttaaaaaat ttatttgcag gatcattagg tgaggaatta
    47941 ggttggagag gatttgttca aaatcatctt cagaaaagac attctccttt aaaagcatcc
    48001 ataatagtag gtttttggtg ggggatgtgg catttaccaa tatggtttac aacaggattc
    48061 aaaggacttg atttaataaa atatattata ttttttatga ttgcaattat atcaatcagt
    48121 attataatga caacttttta taatctaaat aaaactttga taatacctat tgtaatacat
    48181 caactattta attttttaat agggataatt aatggagatt taatagagct tataaattac
    48241 tatgcaatgt tatatttaat atttgcaatt gcattaatag ttataaatcc aggaaatgta
    48301 ttatataaaa atattagaga gtaatatata tctttatggt aagatataaa gatattatta
    48361 gaagaacatc caaggaggaa taaacatggt tttactgatt gttgatgcac aaaagttaat
    48421 aacaaacgag aggttatata aatttaatga atttgtagta aatgtagaaa atttaattga
    48481 tacagctaga aaaaataata tagaagtaat atatgtacgt catgatgatg gagttgaaaa
    48541 tgaattgact aaaggtaaaa atggctttga aatatacgaa aaatttaaac catgtaatgg
    48601 agaaaaaata tttgataaaa aagtaaatag tgcttttaaa gaaactggtc tattagaata
    48661 cctaacaaat aatggtgaaa aagatattat tattgctggt ctccaaacag attattgtat
    48721 tgatgccact ataaaatgtg gatttgaaca tggatttaat ataatagttc cagcttattc
    48781 aaatacaact gttgacaatt tttttatgtc agcagaacaa tcatataaat actacaatga
    48841 atttatatgg aatgaaagat atgcgaagtg tatttcatta gaggaaacac ttagaaaaat
    48901 gaaataaaaa tagagtatga attgtatatg tacataagtt gatttcataa aaaaggatgt
    48961 taaattccaa gttaaaaatt agattaaata tattatatta tagataaggc acatcaattt
    49021 tattttaaat taatgtgctt tttaattgta gtaatttatt tataatcaat atgattttta
    49081 ttatttaaat caatttttct tgagtttctt tgtctaaaga aacttttttt atttataaaa
    49141 tctacagtta tattatagta tctattttag agaagtttaa tttttgtatc aatctaaaac
    49201 aaaagttata cttaaatgaa attgaaaacg ataaaattct caaataaaat aagtattgtg
    49261 gaggtgatga aagaatccaa tgataatatg aagactaatc ttaataagaa agtaggggtt
    49321 aatggtagtg gacaaagaaa ttgtatcaat tctccagatt ttattaaata gcacattgat
    49381 tacaactaat ggattacaag aggagtctaa ttcatcaaag agacaaatta cttatagaat
    49441 aaataaaatc aatgacatgc ttaaagtaaa aaatgtacca cttatatgtt taagagctga
    49501 taaagatatt atcgttagaa aagaaacaaa aaaagcaatt aaagaaattt tagaaaaaaa
    49561 ctattctaag aatacttatt attttagtaa gacagaaaga cttttatata tgtatttaat
    49621 gctttttatt aacatggatg atatttcaat taatcatttt ataaattcta taaaagtaag
    49681 taggtctact gtaaatcttg atttcaagga tttgatacca gagcttgaaa agaagaacat
    49741 taaagttaag aacaatcgta taaatggata ttacatagtt ggcaatgaga tggaaattag
    49801 gagagtgctt attaaaaata ttatagaaac tttgtctaat gagaaaagtt caagagtatt
    49861 tgatattttt attaaagaat ataatttaga tagttttgaa gaggctaaaa aaattatatt
    49921 aaagctaata aaaaaatatg aaattacttt tgttgaagat agattagtag aatttatata
    49981 tatatttatc tttctaaagg caaggatgta ttcaactaga atagttactc aggaaaatgt
    50041 agatattcct aatatttcag ttatgagttc aatgaaggag tataaatttg ctaaggaatt
    50101 attagaagta tatgggagtg aagataagat aaagcctcac aatataatgt atattagtgc
    50161 ttggatttta ggtatatcag taggaaatta tgaagaagat acagaagata aggatgtaat
    50221 ttcaaaaatt gtaaataaaa tgatgacaaa gtttggatat ttaagcggag tttactatat
    50281 aagccaagaa aaaatattta aacaattata ttctcatttt agacctgcct actatagact
    50341 tttatttaaa ttacctattt ataatccgat ttgtgaaaaa gtaaaagaag aatataaatt
    50401 agtttataga attgtaagta atgcaatgaa agagttttgc ggattatttg gagaagaaat
    50461 tcaggaagaa gagttagcat atcttacaat gcactttgca acactttttt caaataaaaa
    50521 agaatttgat ggttttagaa aaagaatagc attaatagtt tgttcaaatg gcgttggtag
    50581 ttcagctatt ttacatgcag aacttacaaa cttatttcct gacttacact ttataccaat
    50641 gtcggaatca gttggattag aaaatataag tgaacctgta gatattgtat ttactactaa
    50701 ttataacgca gagggtttaa aaacagatgt accagtaatt aaagtaagcc ctgttatgac
    50761 cacaaaagaa aaatataaga ttacacgtga agtgtatatt caattaggtg atattttttt
    50821 aagacaacct aaaatagatg aggttatgga tataataaaa aaatatgcaa atgtaacctc
    50881 tgaaagtatt ctttcaagtg agctattagc ttattttaca cagatagaga attttacatc
    50941 taaggaagga gaggggccta tgctaagtga gattactaat gaaacactag taaaaataaa
    51001 ggtaaaagct aagaattggg aggaagctat tagacagtca gcttctgtat tagttgaaaa
    51061 taacaaagta actgaagggt atgtagatgc tatgattaac tcagcaaaag catctggacc
    51121 atacatagta attacaaaac atgttgcact tccacatgct agaccagaag ctgggtcaaa
    51181 agagattgca ataggcattg ctactttaga tagtcctatt gagtttggga ataacgaaaa
    51241 cgaccctgta aaatatgtat tttgtctaag tgcaatagac aataatagcc atttacgtgc
    51301 tatgtctgag ctagttgaac tattggaaga agatgagttt tttaaggttt tagatagtgc
    51361 taaagaagct aaagaaatta ttgattttat aaaaacacac gaattataaa ggaggattta
    51421 attatgtata aaattttaat cgcatgtaga gcaggagttg gctcaagttt aatgttaaaa
    51481 ataaagactc aacaaattat taaggaaaat aactttccaa ttgaagtaga acatggttca
    51541 ttggattcac taaatggatt caatggagat gctgtagaaa cattaattga tgttgctgaa
    51601 gaattaaaaa ataaaaattt gaagtttgat ataattggga ttcacaatat tgttgaccgt
    51661 aatgaaataa agacagcact agaaaaattc cttgcttcaa aagagggata aatttaagat
    51721 gataaggagg aatatttatg gagtttatta ttagtttgct gagtacacct gctgttttgc
    51781 ttggcgtagt tgcatgtata ggattgattc ttcaaaagaa atcagctgtt gaagtattta
    51841 caggctcttc aaaaacactt atcggttttt tgatatttgg tattggtgcc tctgctatga
    51901 caggagcact tcaaaatttc aataaactgt tccaacatgg ctttggaatt actggtgtta
    51961 tagcttcacc agaagcagct acagcacttg cacaaggtac atatggtttt gcagtttcat
    52021 gtacattaat tcttggattc atattaaatt tagtatttgc acgtattaca aagtttaaga
    52081 atattttctt tacaacaggt catagtttgt tcttttcttg tgtattagtt ttaattatga
    52141 aagcacatgg atttgataat acaatcacta ttttaatagg tggaactatc ttagggttta
    52201 tgtctgctgc actacctcaa ttctgtcaac catttatgcg tgaattaaca ggtggcgacg
    52261 aacaagctat tggtcacttt aacatgattg gttatggttt atcaggttat attgggcgtt
    52321 tattttcaaa acataaagat gatacaacag agactataga atttcctaag tggttatcaa
    52381 tattcagaga cttcttaatg ggtctatcca tagttatgtt aatcttattt tatattgcaa
    52441 cattaaaagc tggtcaagca tttactcaag aaattgctgg ttcaacacat tggttggttt
    52501 ttccgcttat agaggcattt acatttgtgg caggtatgtc aatattgatg tcaggagttc
    52561 gtatgttctt agctgaaata actgcagcat ttgtaatcat ttcagaaaag tatattccag
    52621 gttcacgtcc tgcgctagat gttccaaccg tatttccata tgcaccaaat gcagttatta
    52681 ttgggtttat atcagcatat gctgctggtc tattagcaat tgctattatg gcatcatttc
    52741 ctagtatatt tcctgtagta attattccag ctgctcacat atgtttcttc tcaggaggta
    52801 cagctgcaat atttggcaat acttcaggag gatggagagg ggctattgct gggtcatttg
    52861 ttgtggggtt attacttgca tttttaccag ttatactata tccagtttat gggactcttg
    52921 gtatagaagg agcaactttc cctaatattg attacaacgt agttggttcg atattacata
    52981 atattttaca atttataaaa cctatttaga ctaaattata taaaagtata gtcatagtta
    53041 ctatgatttc aaggaggata catcttatgg aaaatacatt atctgaattt ttagataata
    53101 tatctaatga attgtcagaa tttattgcct caatagatga gacatctata aataaagcat
    53161 gcgaattaat tcttgaagct gaaaaaaatc acggacgtgt acatgtgact ggtattggca
    53221 aaccaggaca tgtatcggga tatatttcat cattgctttc atctactgga acgtcagcat
    53281 atatattgca tggtacagaa gcagtacatg ggtcaagtgg tcaagtagtt gaaggagatg
    53341 ttgttattgc aatatcaaat agtggtgaaa ctcaagagtt aaaagcaact cttaagactc
    53401 taaaagtaaa tggagcaaag ataattggtg taagtggtaa tgagagttca tttttaaaaa
    53461 atatttctga tatattcctg tttgcaggtg ttaagcagga aggagattgt ctcaataaag
    53521 ctcctcgtgc ttcaatttta gctgaaacaa taatattaca atcattaagt gttgtattac
    53581 aatatgctaa aggtcttaat acacaggagt atttgaagtg gcatccagca ggaagtcttg
    53641 gaaagtctat tagagaggga atataatttt atgaaatata aagggattat atgtgcaatg
    53701 ataacaccat ttgatgaaaa tcaaaatatt aattcacaag caacatgtca attaattgat
    53761 tatttgattg aaaagggaat ttatggatta tttattttgg gcactaatgg tgaatgtcat
    53821 gtattaacag atgatgaaaa agttgaattt gcaaagattg ttattaatca tacaaataat
    53881 cgtgtacctg tttttgttgg aacagggggt aattcaactc gtgaagttgt agatttatct
    53941 aagagaatgg aagcgattgg agcaagtgca ttatctatta ttacaccata ctttgttaca
    54001 ccaacacaac aggaattaat cttacattac aaaactattg ctaattcagt taatttacct
    54061 attataatgt ataatatgcc aggtaagact ggaattaata ttgaaccaga gtcagtacgt
    54121 gaattatcaa agataaagaa tattgttgga attaaggata gtagtggtaa acttgacaac
    54181 atgaaagctt atattgaagc aacgaagaat gagaatttta gtgtgttttc aggctcagat
    54241 tcacttattc tggatagtct aaaagctggt agccaaggag ctgttgcagc aacaacaaac
    54301 ttcctcacag agattggtat ctctatctat gaaaacttta tgaaagggga tttggaagaa
    54361 gctcaaaaat ctcaaagtag tatagaagaa cttcgacgta tactgaaact tggaacaatt
    54421 ccatctgtta tgaagaaaac tattgtcttg aatggaatta atgttggaac tgcacgactt
    54481 cctgtaactg aaccaactgg ggaagtactt gaagaaatta aacgagtagt tgaaaattat
    54541 aggacaatac ttaataaaag tagtgagaat agataatata aatagggaaa ttacactggt
    54601 atataattaa tatatatatt ataaaacatg actaaaatta atgaataagt aaagtggttt
    54661 gatgagtata gaggtgataa tatgaaaaag atgaatataa ccaaaagaat gtcagaatgt
    54721 ggggctcttg caattgtaag agaagaaaat cttaaccgtg catgtgaaat tgctgaagga
    54781 tgtataaaag gtggtataac agttatagaa atgagttata ccctaaataa tgctggagaa
    54841 ataattcaag ggcttaataa aaagtatggt gaaatacttt gtgtgggagc aggaacagtt
    54901 ttggatagtg aaacagctcg tcatgctata ttacatggag cccaatttat tattgcacca
    54961 aattataatg aaggtgtagc gaagatttgc aatagatatc agattcctta tgctccagga
    55021 tgtacatcat tgactgaggc agtagacgca ctaagtttag gagcaacttt tataaaagct
    55081 ttcccaatat cagatttcta tggacctaaa ctagtaaagg tatttaagac accaatacct
    55141 gatatgccag ttttagcaag tggagggctt aatattgaaa acctacatat atggttagaa
    55201 aatggagttg atgtatgtgg atttggtggt ttattaacta aaggttctgt tgaagatatt
    55261 gctaaaaatg ctaacaagat tcgtgaaatc attaaaaatg taagaaaaat ttaacatagg
    55321 gctgtatcat agtatcttat aattactatg atacagcctt tttaatatta ttcaaattta
    55381 aaacagttta catttctatt atattacata taatttcagt aattttatct taatttttct
    55441 atctggaaaa aaatgattta aaacttaagt tatgatataa tttcattaaa agaaggcgat
    55501 ttttagtaaa tatgagaaaa taatgtgttt ttaggggaaa agttaaatac agatttaagt
    55561 gaacaagaat cgataattaa gatattaaat ggtgaatagg gcatcaaaaa aattactaaa
    55621 ttaaaagttt tagagggaga atcttagcaa gtgtgtgtga aaattcaatt tcaaacaaaa
    55681 ataattgtaa atgaaatcaa tttatacacg ttgaagattc acttttatca ttatattaaa
    55741 aagcagagtg aattaaaagt gagtctaaag ataggaggat aattaaaaca atgaaagaag
    55801 acattaggtt acatcgaatg tgtaaaaaag aacaagcaac aaagaatgaa gttatatcag
    55861 aggatattct aaaatctatg agtattgggg tcagtaaaca ttttttagat gattcattta
    55921 caataatatg gggaaataca tcattctatc aattattagg atatacagaa gaagaatttt
    55981 tagttaaatt ttctagttta aaagattatt atgaacagaa tctttatcat tttgaatcta
    56041 tgaaaaatca ttttttagag gcttatcatc atggcgaaaa gagtatagaa tatcaaattt
    56101 gcatttcagc aaataatgga gaccctgttt gggtaataat gactggtata ttaattgaat
    56161 cacaagataa tgagaaatca atggtctata tattgtattc aaaaattaat gagttaaaaa
    56221 taaagcaaga agaactatct gatatggaaa aagagagtac agagaatttt aaatggatga
    56281 tgtcagagta tgctgggaat gtttatatta gcgatatgga tacatatgaa ttactatact
    56341 taaataagca ctcatgcaat actttacagg catcagcaaa tgaacttatt ggaagaaaat
    56401 gttatgaggt aattcaaggt agaacttcgc catgtccgtt ttgtacaaat tcgtatttaa
    56461 aaaaagatga catatatgaa tgggaatttt ataataaaaa actgaaacga acatttataa
    56521 ttaaagaccg tatgttgaat tggcaagggc gaagagttcg tattgaatta tcttatgata
    56581 tgtatagtgc tgaatataaa ttagcaaaaa aagatcagga aagagaatca attttgaaaa
    56641 ctattccagc aggaatggtt agattagatg caagagatta tcttactata ctttggtgta
    56701 atgatatatt tttgaatatg attgaatata caaaggaaca gtttgcagag gaacttaata
    56761 atcaatgtgg atatttattt tctgaagatt ataaacgtag taagaaactg gcacaagatt
    56821 tgaaggagtc tggggataat gttgtatttg aagcaaaaat atatacacgt tcaagggaag
    56881 aacgtatatg gactgttaca ttgtgttata tcagtgggga agacagttgg gatggaatac
    56941 catcttttta tagtataggt attgacataa caaaggaaag aaaacagatt gagaaattac
    57001 agcatatatc agagaaagat gcattaacag gaatttataa tcgtgctgag actgaaaggc
    57061 agattaagaa atattttgaa aaaaatttaa atgttatggg tgcactgttt atgattgata
    57121 cagataattt taaacagatt aatgatacag aagggcatat gattggagat atagtactta
    57181 ctgagatggc atcaggtatg aaaaaaataa tgcgtgacag tgatgttgtt ggaagaatag
    57241 gtggagatga gtttacaatt tttatgaaga atatttcctc agttaaagat gcagaaaaga
    57301 aagcagaaga actattgtat atgttccgtc atctatttca gaaagaaaaa agttttctaa
    57361 aagtaacttg tagcatagga atagctatat atccaaagga tggtactaca ttcaaggaaa
    57421 tatatgcaag agctgataaa gccctgtatc aagcaaaaaa tatgggaaaa aataattatg
    57481 taatctacaa ccaagattat tttaaagaat tagaagaatt tgattgttct tctcttggta
    57541 cagttattga ttcagaaaaa caatacgctg aatatcctga taatcttaca cgttatatat
    57601 tccgtatgct ttatcagact gatgatatag accaggcgat taatatgata cttgaggttg
    57661 ttgggaaaca gtttgatgta agtcgtgttt atatttttga aaatacagaa gatggtagat
    57721 atacttctaa tacttatgaa tggtgtaatg aaggaatctc ttctcaaatg gcttatttgc
    57781 aaaacagcaa ttatcaagac tatgaggatt atgaaaagct ttttgaggat gaattagttt
    57841 tttattgtcg agatatccat acattaagtc ctaaactaga agaattgttt tctagtcaag
    57901 gtatccactc tacacttcaa tgtgcatata aagaagatag agttttcagt ggatttgttg
    57961 gatttgatga atgcacagga ctacgtctat ggtcacaaga ggaaataagt acattatcat
    58021 tgatttctca aattatatca atgtttttac aacgtaaaaa aaataataaa cttaatcaac
    58081 agattcaaca ataccagaat attttagata atattgacca gtgtatttgt gtgattaata
    58141 aagaaaataa ttttttatta tatgtaaata aaaaatttaa aaatacatat tctaaattcc
    58201 aaattggaca accttattat cttgattcta tagatattca taagatatcc attatatgga
    58261 ataaaaaaga agcataccta tgtactagca cgatttaatg gtaaagtaaa gtttatattg
    58321 gacttaaatt aaatttcgtt ttaaataaga tattatttta gttgcttact gaaaagacta
    58381 gaatctatat taatgaatta tactaatttt ataccactat taaaatattg tttgctaaaa
    58441 ataaaaaata gccttaaaac agttgattat aaaaaattaa ctgctttaag gctattttta
    58501 tctttgtgac gaaatagaca cttgccattt agaatgtgac aatatagtag ttgtttagaa
    58561 aaggcacaaa gcattatact ttaactaaag taaaatgtat tttgctttta gaataaaaac
    58621 aattaggagg gagctataat tgaaaaagat aatatatgta ttatttacaa ttgaactatt
    58681 aatagtatct ttattaggtc taaatatagt taaagataat gaaattaata atattttata
    58741 taacgataca acaagtatat ctgtaatgtt taaagattat aaaaagttaa ataaaaatta
    58801 tagtaagtgg attaaagata ttgcagatga aaataatgtg gctatatcaa agtatgtttt
    58861 taataataat gaaaaattat ctatatatac gactgataca agtttgaata acaatataaa
    58921 gttaaaatca ggaaaatttc ctaatactaa ttcaaatgaa tttattagta atataaacac
    58981 taataatagt aatcaagtag gtaaattctc aactatgagt aaagacatat caattttaat
    59041 tagggatttt ggtaaaataa gtgaagttgg agaatcagga atcttatata tttcaactca
    59101 aaatgaagat acaataaata aaatattaag agaactaaat gtagacagaa gtatttttgt
    59161 agcaagatta catgatagat atgttaactc aaatttatac ttaaatcctt caataattag
    59221 agatttaata ctaattatcc tttgtttctt ggctacaata atacattatt caattagtgt
    59281 gagtagagag gcttctattt taagattaaa tggatactct aagctagata taattgctag
    59341 agttataaaa aatatactta gaattatgat tttatcatct gtcacagcat tattaatata
    59401 tataatctat attttaaaat taaatatagg tgttagagca tgcttatact ttgctatgtt
    59461 ttcgattata tgtattttga taaatatttt gatatctata ttaatagttg gttttaatag
    59521 tagaagttct aaatatgtgc tatctctaaa tggaaaaaaa tcttatggtt ttgtaaatat
    59581 aatgcatttc acattgaaaa ttgtatttgt atcatttctt atattatcca taaataactg
    59641 tattttaaat tataatatgc tacaaactca gttaggaaat ctatctgagt gggataaagc
    59701 aaaaaatgtt tataatctta ctcttaaaga tacaggcgaa caatctttag gaaaggaaga
    59761 agtaattaaa aatgccaaga ttaaaggttt ctataaaaat ttggtagaag aaaaagatgc
    59821 tttcttaata gattcaacta attttgaaaa acttgaagat ggaagttaca tgtatgaaat
    59881 aaattcaaag ggtaaaaacc cagaagtaag tcaatatggt aaaaatataa aaataaataa
    59941 aaactatctt aaagttaacc ctatttactc aaatggcaaa gaaatattta atctaatcga
    60001 ttatgacgat aagactataa atttattagt tcctaaaaag ttacaagtac atgaaaatga
    60061 gattaaaaaa gagtttagag aattatttta ttttgaaaaa atagaagtag aaaatatgta
    60121 caataaagag ttaaatagag agttaaataa aacaaagaag tccgatttaa atgttaatat
    60181 aatatatgta gataataatc aaagctattt tacttataac tcccttgtta tggatgacaa
    60241 taggaatctc attgatgacc caatagcaat agttgacata gataatattg atgattcttt
    60301 ttatctttct tatatatcta gatgtgtata ttttaattct aagaaattag atgcccttgc
    60361 agatataagt agcattattg aagctcaagg tgtagaagcg catatccaaa gtttacatgc
    60421 tgtttataat gagtatggtt tagaaataaa taaactagaa aaatatttga atagtgaaat
    60481 ttttactata ataataatag caatatcaaa cttaatgatt acctacaata tagtagctag
    60541 ctactatgaa agaaataaat ataagttata tattaaaaaa atatttggtt actcaactac
    60601 agctagaagt atgttgttaa tagtctcatt aatattaaca aacattattc caataggcat
    60661 tatatcaaca agagtagatt taagtaataa tattatctta tttggacttc taatattagt
    60721 tatagaagta attgtatcta ttgtattaga caagattatc tcaaatagtt catttaataa
    60781 aattataaaa ggagaacatt aaaaagatga tttcattaat aaatatatct aaaaaattta
    60841 aagataaatc tataatagac aaatttaacc tagaaatttc taagggcgag tttgtagcga
    60901 taacaggaaa tagtggttca ggtaagacaa ctttacttaa tataatggga ttattagaaa
    60961 aacctaattc tggtgatatt ataataaata atataaaaaa tccaaatagt aaacagataa
    61021 gaattttaca aagggatttt tatggctatc tatttcaaaa ttatgcttta attgagaatg
    61081 aaaatgttga aaataacttg aaaattgctt taaagtatca aaaaaatatc aatcataatg
    61141 aaaaaataaa ttacgcactt gagtctgtag gtcttagaaa atacaataaa aaaaagatat
    61201 acgaattgag tggaggagaa caacaaaggg tagcattagc aagagtaata ttaaaagaat
    61261 gtgaagtaat atttgcagat gaacctacag gtaatttaga taaaaaaaat agagacatag
    61321 tatttgatat acttaaaagt ctaaataaga atggtaaaac tatcgtattt gtaactcatg
    61381 atttagattt atcggagcag gcagatagag ttataaaatt gtaataatat aaactataat
    61441 aaatataaga aagagattac agctaaatta agtttaataa taatacgaat agaatttaaa
    61501 cattaagtaa attagtagtt tgtaaatata agtataatat gatatcataa aggctcaaaa
    61561 gaaattttat ataataagaa atctaaaaat aacaaatgga ggacatataa agatggaaaa
    61621 tttaccaaat tgcccgaaat gtaattcaga atatacttat gaagatggaa ctcttttagt
    61681 ttgtccagaa tgctctcatg agtgggcatt aggattggga gctgaagaag attcaaatat
    61741 tatcaaagat gtaaatggaa atgtattaaa tgatggagat tctgttacag taattagaga
    61801 ccttaaagta aaaggaagtt catcatctat aaagattgga acaaaagtga aaaatatccg
    61861 tttaatccat gactcatcag atggacatga tatagaatgt aagatagatg gttttggagc
    61921 tatgaagcta aagtcttctg ttgttaaaaa agcgtaatat tataaataag ctgtagtgtt
    61981 tattgttttt cagtacagta taaaaccata agaatctatt aagtaataaa agtttgttta
    62041 tttatcttta tattttctta tggttttatt aaatataaat tttattgaac accaaaatat
    62101 tctctcctct acaaataagc caatcttatt taaactaata tactttgtac tatttatact
    62161 gaaattgtaa atttaaatgc tatgttattt aggtttgtat agttaattta tatatttctg
    62221 attttataaa ttgtatttat tattatatct tgattctaaa atcattaaaa atttcaactt
    62281 ctataaagat ttacaattta tcaaatacat tatgagtata taaacaattt attattacag
    62341 tgctacattt aaaataagtc ttagtataaa ggtattgtta caagtataaa gaaacttatg
    62401 tatgctattg gaataaataa taccttaaat tataattaag aataagaact gttaaataag
    62461 ggacttgatt atcatttagt tatagataat ataatattaa aaataaagtt taagaggaga
    62521 aaaaatatga tagatataca taaaaatata tatgataata agttatttga ggagctaaag
    62581 attgactgta agaagtgctt tggactttgc tgtgtagctc tttatttttc tgcttcagat
    62641 ggatttccta tcaataagga atcaggaaaa ccctgtatta atttacaacc agattttaaa
    62701 tgttctgtac ataatagtct tattaagcga ggttttaaag ggtgtactgc atatgattgt
    62761 tttggttcag gtcaaaaagt agcacaagta acatataagg ggatagactg gatgcaatca
    62821 ccagaactaa caaatcaaat gtctgaagtg tttttaatta tgagacaatt acatgaaatg
    62881 ttatggtatc ttaaagaagc atctgtatta aatatatcag atacaatcaa atcaaaaatt
    62941 gatttaataa ttgaagaaac tgaaaagata actaatatgg gcccagaaca gataattaat
    63001 ttggatatca tatcacatag aacaaaggtt aatttattat tatcacaagc gagtgagtct
    63061 atgatgggaa aggttaaatc atttatcaag acttctactc ttaaaaacat gaaaaaatta
    63121 agtaaaaata tagatttaat aggagcagac cttagaaaaa taaatctaat aggagcagat
    63181 ttaagaggta gatttctaat agcagcaaat ttaagaaata cagatttgag tggggcaaat
    63241 ttaataggag cagacttaag agattgtgat attagaggtg ctaatcttga aaatagtata
    63301 tttttaacac aattacaagt aaatactgca aaaggagatt ctagtacgaa gttaccagct
    63361 tcactaatac gccctaaata ctgggaaaaa taattaaatt aatacataat tttacaaaat
    63421 atgaactttt ttgactttaa ttcatattat acattagaga ttctttacaa gaatttctta
    63481 atataatata gggagagata taaaaatgag ttatcaaaat tataaatgtg aaagagatta
    63541 tgtgtatgat gattataaat acaataagtg caacaagtgc tataatgatt atgaggaaat
    63601 gacacatgtt catgaatatt cagaaagtgt taagttagca gaagaatgtg aggatagaca
    63661 taaccaccgt gcagcagggg ttactggtga agttatacca ataaatggag gtacaaatca
    63721 tgttcataaa ataaatgata atgtagattt ccttgaccat ttccataaga tatgtgttac
    63781 aacagggcca gcaattagaa tacctggaac tgataaacac attcacttaa tatgtggaga
    63841 aactactgtt aatgatggtc attgtcataa attccttttt actactcaaa tagaagcacc
    63901 tttagtataa gctgatgtga attattgtag tgaatttgct agattatagt ataatatata
    63961 atagataata tttaaactta atgaggacta tgatatttaa ccttcgtagt cctttctaag
    64021 ttttgtataa aaaagtgtta tgcaattaaa agtgatatag ggaggaaatt tttatgaaat
    64081 ctggaataat tcttgaaatg aaacaaataa attcaacaga atatgacgaa atgttgcaag
    64141 tctgggaatc atcagtaaga gcaacgcatg attttttaac agaaaaagat attgaatctt
    64201 tgataccatt agttgagatt ggacttaaag aagtagaaaa tatagtttgt ataaaagata
    64261 atgatataat aaagggcttt ataggaatag ataaagataa aattgagatg ctctttatag
    64321 aagataaata tagaggaaat ggtattggta aaaaattaat aaaatatgct atagataaat
    64381 acaatgtaaa atatgttgat gtaaatgagc agaataaaaa agctgtagga ttttatattc
    64441 acttaggatt taaagtgttt gataggtctg aaatagatgg acaagggaac ccatttccaa
    64501 tactacatat gaaattaatt taaagttata cttcccactt attcgcaaaa atatttactt
    64561 aaatttttat acaaataaaa gtcgtgacag tcttaggaca cgactttttt actacactat
    64621 taaattgaaa tttatctgtt ctatattatg attattagat gtagtttttt tattttaact
    64681 atgtacttaa ataccaagta tacccatacc tatcaagtac ttcagcagcg ccatcatttt
    64741 gatgtggtgg tgggtgtggt aaaaattctt gtacaagtgt accaccttcg gcaagtttta
    64801 cagatatttc ttctatcgct tctcgagatg gtagaacaac acaaatcgcc atgccagaaa
    64861 ttacaacagg cttgtctgta tccgcaccca ttatacaacc ttcgtatttt gtgaaagtaa
    64921 cacttgcgtg cataatagga ttgtttggat tgctgtctaa tcgcgcaaat atagtttttc
    64981 ctcctccgaa agcctcagca taaaaattaa atgctttttc acaagtacca ttaaatatta
    65041 aatatggagt tagcatgacc aatatatcct ccttgatata gaataattta tagttgcacc
    65101 tgataatcta aattatgatt tagtaaattg tatcataggg aaaccttgaa gtatataatt
    65161 ttttattatt atattataca aatttattaa tttaaaaaaa tgttatatgt acggaaggtt
    65221 tacaggataa aatataataa aaagattaag gtgacattat aaaaaatgta tattgacatt
    65281 gacgctacgt aattgtatat actttgtctt aaaggagatg gtaaatatga atattataga
    65341 aattcgctca gataaaatat acaagaagat aatggatgca ccaataaaca aaaaagaaga
    65401 tatatacaga tatgaattaa tgaagccttt tgaatttaag tggaagtgta tgaatgttcc
    65461 aatagttgct agacagaaag gtggatatga tgtaattata gcaagtgaaa tgttaggggt
    65521 tttatcgcct aaggatattg atgaaaagca aaaaaagaat ataaatgtgt tatctgctga
    65581 taaaatttgg ggcacttgca aagaaaccat agaaaactct ataaatgctt ttataaaaga
    65641 agggtatgat ttaaacatta aggactataa atattcaata ttattggcga atccaaatag
    65701 tccttataca atattaagtg atggatactg gggtgatggt gggattcctg gatatatatt
    65761 tctatcattg gttcctaatg aatatactat caatagatta ccagtattaa tagcacatga
    65821 atgtaatcac aatattagat ttcagtttat agagtggaat aataatataa cattagaaga
    65881 aatgatgata aatgaaggtc ttgcagaaaa ttttgcaaca tggatgtttg gagaggaaat
    65941 gttaggacct tgggtcagta gaacagatat cgaaacatta aatacttata taaagccaat
    66001 aataaaaagt gctttaaaag aaactggatt tcaaaatata acatcttatc tttatggtga
    66061 tgatatagct aaaatgcaag gatattttcc agtagggttg ccttattgtg caggatatgc
    66121 ttgtggatat tatatgatta agtattattt agaaaagaca aataaatcaa taatcgaagc
    66181 gactttattg ccttatagtg agataatcga agcagtaaaa gagttttggg aataaatttt
    66241 ataaattgca taatttgaaa taaattatgc aattatttta tttttaagaa aaaataaatt
    66301 gacaataatt tataagaaat atacaataat atatataaag aaatatttca atatttaaaa
    66361 gttttaagat ttcatatcta gagtagctaa cattacatgt tagtttcttg ttacttatct
    66421 aattacttta tatttcgtag ttatatataa aattactata aaaaatgaaa tatattaaac
    66481 aatcaactga aaaatgaaat gttaataaaa aaaaattcaa attataaata ttaaatgaat
    66541 ataattaaaa caaatatata aatttaagta taacactcac attttaatgt attgtgaaat
    66601 ttttgttgaa ctttatgtac cataacttat aaaatttatg tcatgttata tttgaataag
    66661 taataaaatt gtagatgtaa attaatctac aaaatttata tgaaatggag ttgatgctat
    66721 gacagaatta atgattataa gtgggttagt actattgatt tgtataactt ctagcaaggt
    66781 attgtataaa tttggagtac caatattatt gatttttatc ttactaggga tgttatttgg
    66841 gtctgatggg atggtaggaa tctattttga taactatgaa ctgacaaaac aattatgttc
    66901 tattggacta atatttataa tgttttttgg agggtttgga acaaactgga atatggctaa
    66961 acctgttgct ataccttcta tattaatgtc ttcactgggt gtaataatta cggctggggt
    67021 tacaggtatg ttttgccaca tggtaatagg aactagttta cttgaaggtt tattaattgg
    67081 ggcaattgca gcttcaacag atgctgcatc tgtgtttgct gtgttgcgtt ctcaaaaact
    67141 aaacttaaag ggttcacttg catcattatt agaagttgaa agtggaagta atgacccaat
    67201 agcgtatatg ctgacactaa ttcttttgac attgatgaat aattctgggg tagaacttat
    67261 tgcaccaatg gtaataaaac aaatagcttt tgggttagct ataggattta tactagcaaa
    67321 attatctgtt tatattttaa gacattcaaa cttcgagata gaaggatttt atactatatt
    67381 tataacagct attgcaatac tttcttatgc atttagcgag tatcttggag gaaatggata
    67441 tttaagtgtt tacatttcag gtattattat aggaaatagt aggatacctc ttaaaaaaag
    67501 tttggttcat ttctttgatg gaatttcatg gattatgcaa ataatgttat tctttatgtt
    67561 aggattgtta tctttccctt ctaagttacc aaatgtattt ggtatagcaa tggccatatc
    67621 tatattcatg atagttattg caagaccatt agcaacattt atagttctag caaaatttaa
    67681 attctcaaat aaagaaaaat tattcatatc gtgggtaggt cttagaggtg cggcttcagt
    67741 agtttttgca atttatgctg ttactcaagg agtagccata gagaatgaca tattccatat
    67801 aatatttttt atagcgttgc tttcagtagg aatacaaggt tcattgatac caactgtagc
    67861 cagaaagttg gatttagttg atgataatac acctgtatta aaaacattta atgattatga
    67921 tggagaaatt agctctaaac ttatcgaagt aaatgttgat gagggtaata aatgggctaa
    67981 taagagtata atagattcta atataccaga cgatatatta atagttatga taaaaagaaa
    68041 ggaacaggtt ttaataccta aaggttcaac cattataaaa acaggggata ttttggtatt
    68101 gagtggagat agaattgaag aattaataca attataattt attttgtatt gactttaacc
    68161 taaggggaaa gtttaaactt aaattaatca aatctaatgc gcaggtgatg agatgttttc
    68221 tataggaatg ttttctaaaa taaataaaat aactacaaag acattaaggt attatgaaga
    68281 tatagggttg ttaaaaccag aatatgtaga tgaatttaca aaatataggt attatacaac
    68341 agagcaatta cctaaattac atcagataat aactttaaaa caaatgggtt tatcattagc
    68401 tgagataaaa aaagctgtag aaaatcctga agaagttgaa aatattttaa tgaataaaga
    68461 aaaagaaatg ttggatgtaa ttaaaagtga agaatataaa ataattaaac tacgtaatta
    68521 ccttatgaat ttgaaaggag aagtcaatat gaatgatgta attattaagt ctttaccaaa
    68581 agttaaagtt gcttctatga gaaaagtgtt aaaggggtat gatgaacttt ttaatctatt
    68641 tccaaatgta atggctgaag aaatgatgaa aacagggtgt acatgtgcag ttcctgatta
    68701 ttgttttaat atatatcatg atggtgaata tagagagact gatatggatg ttgaaatgtg
    68761 tcaagctata aatgatttaa aggaagatac ggatatatta aaatttaaag aattagatgc
    68821 agttgaaaat gcagtttgta tattacacaa tggtccatat tcaacattag gaaaaactta
    68881 cgcagatgca tttaaatgga tagaggataa taaatataaa gtggttggaa atcctagaga
    68941 atcttatata gatggtatat ggaataaaga gagtgaagaa gactggttga cagaagttca
    69001 gataccagtg aaacgcatgt tataatatgt ctacaaagta taaataagaa taaaaaaggt
    69061 ggtgtctcaa agtaaacatt ttagtttatg agacactctt tttcatatga aatcaagtat
    69121 atttttataa ttacgattat gaaaaaaggc ttatctatat ttttagacag cctcttattt
    69181 ttttattata atagttttct aaagatgctc tatttataaa tttttattaa agactttata
    69241 aataattgta tttaatatac aaaatatata taaatattga tgttttatat ataaagttgt
    69301 atattttttt aaggatataa agtaaaaaaa tatacaatat caagaaaaaa agatattggg
    69361 tatttaaagt aatactttat ttaattttaa gtaaatcggg tatctacata tatagatact
    69421 aatttcaaag
//
LOCUS       DAELTA010000002        68600 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.002, whole genome shotgun sequence.
ACCESSION   DAELTA010000002 DAELTA010000000
VERSION     DAELTA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.002"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="KPG13_000074"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="KPG13_000074"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="KPG13_000075"
     CDS             275..466
                     /locus_tag="KPG13_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG0983825.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="KPG13_000076"
     CDS             621..2030
                     /locus_tag="KPG13_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0983826.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="KPG13_000077"
     CDS             2161..2850
                     /locus_tag="KPG13_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG0983827.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="KPG13_000078"
     CDS             2853..3788
                     /locus_tag="KPG13_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG0983828.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="KPG13_000079"
     CDS             3785..4678
                     /locus_tag="KPG13_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG0983829.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="KPG13_000080"
     CDS             4756..5502
                     /locus_tag="KPG13_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG0983830.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="KPG13_000081"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="KPG13_000081"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG0983831.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="KPG13_000082"
     CDS             6359..7063
                     /locus_tag="KPG13_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG0983832.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="KPG13_000083"
     CDS             complement(7251..7679)
                     /locus_tag="KPG13_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0983833.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="KPG13_000084"
     CDS             complement(7716..9146)
                     /locus_tag="KPG13_000084"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG0983834.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="KPG13_000085"
     CDS             9278..9862
                     /locus_tag="KPG13_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG0983835.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="KPG13_000086"
     CDS             9878..10399
                     /locus_tag="KPG13_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBG0983836.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="KPG13_000087"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="KPG13_000087"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG0983837.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="KPG13_000088"
     CDS             12762..13532
                     /locus_tag="KPG13_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG0983838.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="KPG13_000089"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="KPG13_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG0983839.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="KPG13_000090"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="KPG13_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG0983840.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="KPG13_000091"
     CDS             14894..16009
                     /locus_tag="KPG13_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG0983841.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="KPG13_000092"
     CDS             15999..16868
                     /locus_tag="KPG13_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG0983842.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="KPG13_000093"
     CDS             16861..17727
                     /locus_tag="KPG13_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0983843.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="KPG13_000094"
     CDS             17768..18481
                     /locus_tag="KPG13_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0983844.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="KPG13_000095"
     CDS             18482..19651
                     /locus_tag="KPG13_000095"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG0983845.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="KPG13_000096"
     CDS             19655..20491
                     /locus_tag="KPG13_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0983846.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="KPG13_000097"
     CDS             20492..21487
                     /locus_tag="KPG13_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0983847.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="KPG13_000098"
     CDS             21505..23193
                     /locus_tag="KPG13_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0983848.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="KPG13_000099"
     CDS             23205..24272
                     /locus_tag="KPG13_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0983849.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            complement(24662..24826)
                     /locus_tag="KPG13_000100"
     CDS             complement(24662..24826)
                     /locus_tag="KPG13_000100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983850.1"
                     /translation="MKSLLNSTLISSGLIATMFISSSIKSIVPIKSATPRANLNLNNS
                     SLVKYSYFQF"
     gene            25036..26103
                     /locus_tag="KPG13_000101"
     CDS             25036..26103
                     /locus_tag="KPG13_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0983851.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26295..26825
                     /gene="rnmV"
                     /locus_tag="KPG13_000102"
     CDS             26295..26825
                     /gene="rnmV"
                     /locus_tag="KPG13_000102"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG0983852.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26996..27865
                     /gene="rsmA"
                     /locus_tag="KPG13_000103"
     CDS             26996..27865
                     /gene="rsmA"
                     /locus_tag="KPG13_000103"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG0983853.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27931..29193
                     /locus_tag="KPG13_000104"
     CDS             27931..29193
                     /locus_tag="KPG13_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983854.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29344..30459
                     /locus_tag="KPG13_000105"
     CDS             29344..30459
                     /locus_tag="KPG13_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0983855.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30645..31541
                     /locus_tag="KPG13_000106"
     CDS             30645..31541
                     /locus_tag="KPG13_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0983856.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31611..32408
                     /locus_tag="KPG13_000107"
     CDS             31611..32408
                     /locus_tag="KPG13_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983857.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32463..33815)
                     /locus_tag="KPG13_000108"
     CDS             complement(32463..33815)
                     /locus_tag="KPG13_000108"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG0983858.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34034..34912
                     /gene="purR"
                     /locus_tag="KPG13_000109"
     CDS             34034..34912
                     /gene="purR"
                     /locus_tag="KPG13_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG0983859.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35068..35346
                     /gene="spoVG"
                     /locus_tag="KPG13_000110"
     CDS             35068..35346
                     /gene="spoVG"
                     /locus_tag="KPG13_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG0983860.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35559..36938
                     /gene="glmU"
                     /locus_tag="KPG13_000111"
     CDS             35559..36938
                     /gene="glmU"
                     /locus_tag="KPG13_000111"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG0983861.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37033..37983
                     /locus_tag="KPG13_000112"
     CDS             37033..37983
                     /locus_tag="KPG13_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG0983862.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38821..38906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            39068..39589
                     /locus_tag="KPG13_000113"
     CDS             39068..39589
                     /locus_tag="KPG13_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0983863.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            39740..41416
                     /locus_tag="KPG13_000114"
     CDS             39740..41416
                     /locus_tag="KPG13_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0983864.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41428..42636
                     /locus_tag="KPG13_000115"
     CDS             41428..42636
                     /locus_tag="KPG13_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0983865.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42651..44363
                     /locus_tag="KPG13_000116"
     CDS             42651..44363
                     /locus_tag="KPG13_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG0983866.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44356..45264
                     /gene="pilO"
                     /locus_tag="KPG13_000117"
     CDS             44356..45264
                     /gene="pilO"
                     /locus_tag="KPG13_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG0983867.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45291..45857
                     /locus_tag="KPG13_000118"
     CDS             45291..45857
                     /locus_tag="KPG13_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0983868.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45894..46421
                     /locus_tag="KPG13_000119"
     CDS             45894..46421
                     /locus_tag="KPG13_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0983869.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46442..47980
                     /locus_tag="KPG13_000120"
     CDS             46442..47980
                     /locus_tag="KPG13_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983870.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            48080..49162
                     /locus_tag="KPG13_000121"
     CDS             48080..49162
                     /locus_tag="KPG13_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG0983871.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            49143..49925
                     /locus_tag="KPG13_000122"
     CDS             49143..49925
                     /locus_tag="KPG13_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0983872.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            50027..50677
                     /locus_tag="KPG13_000123"
     CDS             50027..50677
                     /locus_tag="KPG13_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG0983873.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50691..51251
                     /locus_tag="KPG13_000124"
     CDS             50691..51251
                     /locus_tag="KPG13_000124"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG0983874.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51268..54654
                     /gene="mfd"
                     /locus_tag="KPG13_000125"
     CDS             51268..54654
                     /gene="mfd"
                     /locus_tag="KPG13_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG0983875.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54685..55680
                     /locus_tag="KPG13_000126"
     CDS             54685..55680
                     /locus_tag="KPG13_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0983876.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55804..56343
                     /gene="spoVT"
                     /locus_tag="KPG13_000127"
     CDS             55804..56343
                     /gene="spoVT"
                     /locus_tag="KPG13_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG0983877.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56463..58076
                     /locus_tag="KPG13_000128"
     CDS             56463..58076
                     /locus_tag="KPG13_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0983878.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58183..59631
                     /gene="mazG"
                     /locus_tag="KPG13_000129"
     CDS             58183..59631
                     /gene="mazG"
                     /locus_tag="KPG13_000129"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG0983879.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59720..59998
                     /locus_tag="KPG13_000130"
     CDS             59720..59998
                     /locus_tag="KPG13_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG0983880.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            60070..60312
                     /locus_tag="KPG13_000131"
     CDS             60070..60312
                     /locus_tag="KPG13_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG0983881.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60374..60628
                     /locus_tag="KPG13_000132"
     CDS             60374..60628
                     /locus_tag="KPG13_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG0983882.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60629..61162
                     /locus_tag="KPG13_000133"
     CDS             60629..61162
                     /locus_tag="KPG13_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983883.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61168..61452
                     /locus_tag="KPG13_000134"
     CDS             61168..61452
                     /locus_tag="KPG13_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG0983884.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61565..62485
                     /locus_tag="KPG13_000135"
     CDS             61565..62485
                     /locus_tag="KPG13_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG0983885.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62575..64947
                     /gene="spoIIE"
                     /locus_tag="KPG13_000136"
     CDS             62575..64947
                     /gene="spoIIE"
                     /locus_tag="KPG13_000136"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG0983886.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65298..67190
                     /gene="pepF"
                     /locus_tag="KPG13_000137"
     CDS             65298..67190
                     /gene="pepF"
                     /locus_tag="KPG13_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0983887.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67304..68269
                     /gene="galU"
                     /locus_tag="KPG13_000138"
     CDS             67304..68269
                     /gene="galU"
                     /locus_tag="KPG13_000138"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0983888.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
BASE COUNT        27729 a         7339 c        12398 g        21134 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24601 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24661 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24721 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24781 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24841 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24901 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24961 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    25021 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    25081 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    25141 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    25201 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    25261 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    25321 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    25381 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    25441 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    25501 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    25561 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25621 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25681 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25741 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25801 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25861 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25921 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25981 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    26041 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    26101 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    26161 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    26221 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    26281 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    26341 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    26401 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    26461 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    26521 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    26581 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26641 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26701 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26761 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26821 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26881 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26941 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    27001 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    27061 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    27121 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    27181 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    27241 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    27301 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    27361 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    27421 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    27481 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    27541 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27601 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27661 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27721 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27781 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27841 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27901 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27961 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    28021 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    28081 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    28141 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    28201 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    28261 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    28321 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    28381 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    28441 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    28501 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    28561 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28621 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28681 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28741 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28801 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28861 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28921 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28981 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    29041 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    29101 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    29161 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    29221 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    29281 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    29341 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    29401 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    29461 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    29521 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    29581 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29641 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29701 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29761 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29821 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29881 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29941 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    30001 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    30061 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    30121 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    30181 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    30241 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    30301 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    30361 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    30421 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    30481 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    30541 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30601 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30661 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30721 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30781 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30841 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30901 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30961 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    31021 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    31081 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    31141 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    31201 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    31261 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    31321 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    31381 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    31441 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    31501 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    31561 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31621 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31681 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31741 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31801 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31861 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31921 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31981 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    32041 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    32101 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    32161 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    32221 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    32281 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    32341 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    32401 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    32461 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    32521 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    32581 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32641 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32701 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32761 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32821 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32881 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32941 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    33001 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    33061 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    33121 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    33181 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    33241 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    33301 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    33361 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    33421 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    33481 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    33541 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33601 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33661 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33721 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33781 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33841 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33901 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33961 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    34021 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    34081 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    34141 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    34201 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    34261 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    34321 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    34381 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    34441 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    34501 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    34561 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34621 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34681 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34741 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34801 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34861 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34921 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34981 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    35041 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    35101 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    35161 catgatataa aagttataga aggccataat gggctattta tagcaatgcc tagcagaaaa
    35221 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    35281 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    35341 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    35401 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    35461 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    35521 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    35581 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35641 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35701 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35761 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35821 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35881 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35941 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    36001 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    36061 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    36121 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    36181 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    36241 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    36301 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    36361 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    36421 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    36481 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    36541 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36601 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36661 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36721 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36781 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36841 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36901 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36961 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    37021 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    37081 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    37141 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    37201 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    37261 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    37321 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    37381 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    37441 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    37501 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    37561 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37621 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37681 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37741 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37801 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37861 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37921 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37981 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    38041 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    38101 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    38161 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    38221 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    38281 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    38341 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    38401 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    38461 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    38521 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta
    38581 gcaaaggatg gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga
    38641 tatataataa ttgtagggag atgtttcttt ttttgtatag cataattttt ctagaaattc
    38701 tgtttcaata tgtaaaaagt tggtatatat aaatagtata aacagtgttg ctaaaatatg
    38761 gtagatatta tataatataa gtggatatgt atatacatta tgagttatat aatttgaatt
    38821 taataaaata gaaacgttga tttatgttct gtaatgtggg caccttggag catattgagt
    38881 tagtggtgca accggctatg aaattgtatt tatttataaa tactattatt ttcatagctt
    38941 tttttattta aaaaatggtt atatgttaga aataaatttg gttagtacgt ttaaataatt
    39001 ttacttagat ttttaaccag atatattgta agtaagtatt aattaaaaaa tttaaggggg
    39061 aataaaaatg aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt
    39121 aattgcaatt ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa
    39181 caaggctaag gtagcaagtg ttgagtctga ttatagttca gttaagagtg cagcattatc
    39241 ttattattca gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt
    39301 tttagagact tatatggagt ctctgcctga taaagctgat ataggtggaa aatataaatt
    39361 gattaaagtt ggtaataaat tggtattaca gataggtaca aatactgaag gagttacatt
    39421 aacagaggca caatcagcaa agttattgag tgatataggt gaaaataaaa tatatacaag
    39481 cgctacaaca aatagtttgg gaaatccatt aacaagtaat acaaaaatag ataataacgt
    39541 tctatatata gtacttatag ataatactgt gatggacaca acaaaatagt taaaacaata
    39601 tttttataaa aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
    39661 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
    39721 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
    39781 aggatatata acagaagaac agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
    39841 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
    39901 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
    39961 agctacaaaa atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
    40021 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gatagagaag cagttcaaga
    40081 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
    40141 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
    40201 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
    40261 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
    40321 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
    40381 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
    40441 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
    40501 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
    40561 gatagctatg agagttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
    40621 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
    40681 ggttgttggc cctactggaa gtggtaaaac cactacattg tattcaatgt taaatcaact
    40741 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
    40801 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
    40861 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
    40921 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
    40981 tacagcagtt ggagcaattt cacgtctttt agatatggat gtggaaagtt ttttaataac
    41041 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
    41101 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
    41161 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
    41221 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
    41281 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggtatggtta gcttaaaaga
    41341 ggatatagtt aaaaaagttt taaatggaaa gacaacagta gaagaaatga ttagaataat
    41401 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
    41461 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
    41521 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
    41581 ttatctagta gaaagagaaa aaaagactta ggagctgacc agataagcca cttttgtaga
    41641 cagtttgcta ttatagtatc ttctggtata aatagtattt ctggattaga aacattagct
    41701 agaaggtcaa ctaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
    41761 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
    41821 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
    41881 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
    41941 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
    42001 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
    42061 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
    42121 ttatttatct atcagtttaa aaaatatcta aaaacgtcag ttggaagggc tcataaagat
    42181 agagttataa ataagatacc tgttttagga aaaggaataa attgtatggt agctacacga
    42241 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
    42301 tatattatag atagtgtaaa taacaccatg gcagagaaga ttttagcatc agctaaagat
    42361 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
    42421 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
    42481 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
    42541 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
    42601 ttcatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaacaagg
    42661 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
    42721 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
    42781 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
    42841 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
    42901 atatggacaa aaaggattta gacggattta tgaagctaga gatggacgaa atgatggctt
    42961 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacct gataattcag
    43021 atggcgatga atcaagttta aatcttatta ttggagctat acaaaaggaa gaggtaaatg
    43081 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
    43141 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
    43201 gtgacaatag cacaattata aatatatata agaaagatac tttatttatt agtgataata
    43261 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
    43321 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
    43381 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
    43441 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
    43501 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
    43561 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
    43621 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
    43681 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
    43741 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
    43801 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
    43861 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
    43921 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
    43981 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
    44041 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
    44101 cattaactgg aaatagtgag actaatagta ataacacaga taaagatgat aaaactccat
    44161 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
    44221 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
    44281 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
    44341 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
    44401 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
    44461 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
    44521 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
    44581 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
    44641 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
    44701 gacttaacca gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
    44761 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
    44821 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
    44881 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
    44941 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    45001 ttgataaaag agggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    45061 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    45121 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    45181 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    45241 ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    45301 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    45361 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    45421 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    45481 atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg taataattat gaatacaata
    45541 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    45601 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    45661 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    45721 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    45781 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    45841 tgtcaagaat aggataataa gaatttatta aaaaataagt aggttgataa aatatgagta
    45901 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    45961 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    46021 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    46081 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    46141 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    46201 gtgatgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    46261 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    46321 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    46381 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    46441 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttggt
    46501 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    46561 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    46621 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    46681 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gagatatatc
    46741 agatgaagat tttaataatc aagatgagga aaatattgaa gcagaaagac ttggatatat
    46801 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    46861 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    46921 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    46981 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    47041 ggcaaagtta gtgaataatg atattctttt aaaaatgttt ttagaagaaa ataaaattga
    47101 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    47161 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    47221 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    47281 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaaac tattggaaat
    47341 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    47401 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    47461 cttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    47521 gtcttatgag aaaatacctg ttaaatatga taacatggct ttgataacaa cttttgacta
    47581 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    47641 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    47701 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    47761 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    47821 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    47881 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    47941 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    48001 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    48061 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    48121 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    48181 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    48241 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    48301 tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    48361 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    48421 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    48481 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    48541 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    48601 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    48661 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    48721 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag tgggggcagc
    48781 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    48841 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    48901 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    48961 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    49021 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    49081 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    49141 caatggatat tataatcgtt aatctatatg tatttatggt gggtatagtg tttggaagtt
    49201 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttctc
    49261 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ttaagctatt
    49321 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    49381 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    49441 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    49501 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    49561 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    49621 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    49681 gaggagatat caaactattt gcaatgctag gtttttacat gggtgttaaa aatggtcttt
    49741 tgacagcctt actaagcgtt tatgtgggag cagtctatgg aatctgtgta ataattataa
    49801 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    49861 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    49921 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    49981 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    50041 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    50101 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    50161 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    50221 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    50281 agttagtggt ataatagttc aaggtataat ttttttggtc tttttcaata tagttcaatt
    50341 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    50401 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    50461 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    50521 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    50581 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    50641 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    50701 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    50761 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    50821 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    50881 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    50941 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    51001 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    51061 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    51121 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    51181 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    51241 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    51301 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    51361 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    51421 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    51481 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    51541 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    51601 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    51661 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    51721 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    51781 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    51841 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    51901 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    51961 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    52021 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    52081 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    52141 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    52201 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    52261 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    52321 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    52381 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    52441 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    52501 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    52561 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    52621 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    52681 gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat
    52741 ttaagtgttg gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt
    52801 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    52861 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    52921 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    52981 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    53041 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    53101 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    53161 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    53221 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    53281 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    53341 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    53401 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    53461 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    53521 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    53581 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    53641 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    53701 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    53761 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    53821 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    53881 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    53941 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    54001 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    54061 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    54121 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    54181 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    54241 aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat
    54301 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    54361 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    54421 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    54481 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    54541 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    54601 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    54661 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    54721 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    54781 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    54841 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    54901 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    54961 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    55021 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    55081 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    55141 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    55201 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    55261 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    55321 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    55381 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    55441 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    55501 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    55561 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    55621 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    55681 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    55741 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    55801 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    55861 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    55921 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    55981 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    56041 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    56101 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    56161 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    56221 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    56281 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    56341 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    56401 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    56461 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    56521 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    56581 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    56641 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    56701 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    56761 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    56821 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    56881 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    56941 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggct tacttgctta
    57001 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    57061 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    57121 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    57181 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    57241 tagataatgt tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa
    57301 attccatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    57361 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    57421 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    57481 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    57541 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    57601 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    57661 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    57721 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    57781 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    57841 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    57901 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    57961 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    58021 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    58081 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    58141 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    58201 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    58261 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    58321 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    58381 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    58441 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    58501 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    58561 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    58621 tcatatatag acttggatac aagtatgata ataactcagg tatatgataa atttatagca
    58681 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    58741 aatggagctg gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    58801 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    58861 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    58921 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    58981 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    59041 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    59101 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    59161 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    59221 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    59281 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    59341 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    59401 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    59461 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    59521 ataaatagat ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac
    59581 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    59641 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    59701 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    59761 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    59821 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    59881 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    59941 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    60001 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    60061 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    60121 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    60181 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    60241 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    60301 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    60361 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    60421 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    60481 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    60541 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    60601 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    60661 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    60721 aagagggaat tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt
    60781 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    60841 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    60901 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    60961 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    61021 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    61081 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    61141 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    61201 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    61261 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    61321 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    61381 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    61441 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    61501 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    61561 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    61621 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    61681 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    61741 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    61801 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    61861 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    61921 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    61981 agaatttatt gtaggtgatt cagaaggaat tatatttaat aaaagtgaga atgtaggtgc
    62041 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    62101 tatgagttca tttatatatg gtgagattga taaaacgtta aatcatataa aagacaaagg
    62161 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    62221 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    62281 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    62341 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    62401 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    62461 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    62521 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    62581 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    62641 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    62701 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    62761 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    62821 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    62881 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    62941 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    63001 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    63061 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    63121 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    63181 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc tactatggga
    63241 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    63301 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    63361 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    63421 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    63481 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    63541 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    63601 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    63661 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    63721 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    63781 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    63841 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    63901 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    63961 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    64021 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    64081 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    64141 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    64201 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    64261 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    64321 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    64381 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    64441 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    64501 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    64561 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    64621 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    64681 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    64741 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    64801 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    64861 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    64921 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    64981 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    65041 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    65101 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    65161 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    65221 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    65281 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    65341 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    65401 atagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    65461 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    65521 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    65581 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    65641 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    65701 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    65761 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    65821 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    65881 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    65941 ttccaagcca agtatatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    66001 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    66061 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    66121 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    66181 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    66241 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    66301 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    66361 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    66421 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    66481 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    66541 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    66601 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    66661 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    66721 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    66781 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    66841 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    66901 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    66961 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    67021 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    67081 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    67141 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    67201 aattcacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    67261 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    67321 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    67381 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    67441 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    67501 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    67561 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    67621 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    67681 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    67741 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    67801 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    67861 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    67921 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    67981 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    68041 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    68101 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    68161 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    68221 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    68281 tgatgtatgg tggcaaaatc aagtcagaaa agaattaata gaataatagc tgttgggatt
    68341 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    68401 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    68461 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    68521 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    68581 taaatttata aaaacgtgct
//
LOCUS       DAELTA010000003        67570 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.003, whole genome shotgun sequence.
ACCESSION   DAELTA010000003 DAELTA010000000
VERSION     DAELTA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.003"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(446..1168)
                     /locus_tag="KPG13_000139"
     CDS             complement(446..1168)
                     /locus_tag="KPG13_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0983889.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1227..1904)
                     /locus_tag="KPG13_000140"
     CDS             complement(1227..1904)
                     /locus_tag="KPG13_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0983890.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1932..2747)
                     /locus_tag="KPG13_000141"
     CDS             complement(1932..2747)
                     /locus_tag="KPG13_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0983891.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2973..3183)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3901..5967)
                     /locus_tag="KPG13_000142"
     CDS             complement(3901..5967)
                     /locus_tag="KPG13_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0983892.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(6044..9073)
                     /locus_tag="KPG13_000143"
     CDS             complement(6044..9073)
                     /locus_tag="KPG13_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0983893.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(9126..10586)
                     /locus_tag="KPG13_000144"
     CDS             complement(9126..10586)
                     /locus_tag="KPG13_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG0983894.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10678..11052)
                     /locus_tag="KPG13_000145"
     CDS             complement(10678..11052)
                     /locus_tag="KPG13_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983895.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11228..11968)
                     /locus_tag="KPG13_000146"
     CDS             complement(11228..11968)
                     /locus_tag="KPG13_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0983896.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11989..12777)
                     /locus_tag="KPG13_000147"
     CDS             complement(11989..12777)
                     /locus_tag="KPG13_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG0983897.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12865..12955)
                     /locus_tag="KPG13_000148"
     tRNA            complement(12865..12955)
                     /locus_tag="KPG13_000148"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12919..12921),aa:Ser,seq:gct)
     gene            13214..13978
                     /locus_tag="KPG13_000149"
     CDS             13214..13978
                     /locus_tag="KPG13_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0983898.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14259..15374)
                     /locus_tag="KPG13_000150"
     CDS             complement(14259..15374)
                     /locus_tag="KPG13_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0983899.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15415..16941)
                     /gene="glpK"
                     /locus_tag="KPG13_000151"
     CDS             complement(15415..16941)
                     /gene="glpK"
                     /locus_tag="KPG13_000151"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0983900.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            17234..18331
                     /locus_tag="KPG13_000152"
     CDS             17234..18331
                     /locus_tag="KPG13_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0983901.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(18822..19334)
                     /locus_tag="KPG13_000153"
     CDS             complement(18822..19334)
                     /locus_tag="KPG13_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983902.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            19887..20768
                     /locus_tag="KPG13_000154"
     CDS             19887..20768
                     /locus_tag="KPG13_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG0983903.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(21081..21860)
                     /gene="hisJ"
                     /locus_tag="KPG13_000155"
     CDS             complement(21081..21860)
                     /gene="hisJ"
                     /locus_tag="KPG13_000155"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG0983904.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(22406..22756)
                     /locus_tag="KPG13_000156"
     CDS             complement(22406..22756)
                     /locus_tag="KPG13_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG0983905.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(22772..23188)
                     /locus_tag="KPG13_000157"
     CDS             complement(22772..23188)
                     /locus_tag="KPG13_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG0983906.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(23207..23398)
                     /locus_tag="KPG13_000158"
     CDS             complement(23207..23398)
                     /locus_tag="KPG13_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG0983907.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(23549..24364)
                     /locus_tag="KPG13_000159"
     CDS             complement(23549..24364)
                     /locus_tag="KPG13_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG0983908.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(24411..25349)
                     /locus_tag="KPG13_000160"
     CDS             complement(24411..25349)
                     /locus_tag="KPG13_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0983909.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(25346..26218)
                     /locus_tag="KPG13_000161"
     CDS             complement(25346..26218)
                     /locus_tag="KPG13_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0983910.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(26243..28171)
                     /locus_tag="KPG13_000162"
     CDS             complement(26243..28171)
                     /locus_tag="KPG13_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG0983911.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(28283..28660)
                     /gene="gcvH"
                     /locus_tag="KPG13_000163"
     CDS             complement(28283..28660)
                     /gene="gcvH"
                     /locus_tag="KPG13_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG0983912.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(28747..30873)
                     /gene="cdhC"
                     /locus_tag="KPG13_000164"
     CDS             complement(28747..30873)
                     /gene="cdhC"
                     /locus_tag="KPG13_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG0983913.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(30930..31736)
                     /locus_tag="KPG13_000165"
     CDS             complement(30930..31736)
                     /locus_tag="KPG13_000165"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0983914.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(31786..33153)
                     /locus_tag="KPG13_000166"
     CDS             complement(31786..33153)
                     /locus_tag="KPG13_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG0983915.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(33184..34128)
                     /locus_tag="KPG13_000167"
     CDS             complement(33184..34128)
                     /locus_tag="KPG13_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG0983916.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(34148..34918)
                     /locus_tag="KPG13_000168"
     CDS             complement(34148..34918)
                     /locus_tag="KPG13_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0983917.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(34974..36359)
                     /gene="lpdA"
                     /locus_tag="KPG13_000169"
     CDS             complement(34974..36359)
                     /gene="lpdA"
                     /locus_tag="KPG13_000169"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0983918.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(36403..37284)
                     /locus_tag="KPG13_000170"
     CDS             complement(36403..37284)
                     /locus_tag="KPG13_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG0983919.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(37319..37993)
                     /locus_tag="KPG13_000171"
     CDS             complement(37319..37993)
                     /locus_tag="KPG13_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG0983920.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(38030..38902)
                     /locus_tag="KPG13_000172"
     CDS             complement(38030..38902)
                     /locus_tag="KPG13_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG0983921.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(38994..39626)
                     /locus_tag="KPG13_000173"
     CDS             complement(38994..39626)
                     /locus_tag="KPG13_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG0983922.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(39731..41407)
                     /locus_tag="KPG13_000174"
     CDS             complement(39731..41407)
                     /locus_tag="KPG13_000174"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG0983923.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(41656..42435)
                     /locus_tag="KPG13_000175"
     CDS             complement(41656..42435)
                     /locus_tag="KPG13_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0983924.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(42745..44664)
                     /gene="cooS"
                     /locus_tag="KPG13_000176"
     CDS             complement(42745..44664)
                     /gene="cooS"
                     /locus_tag="KPG13_000176"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0983925.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(45271..46182)
                     /locus_tag="KPG13_000177"
     CDS             complement(45271..46182)
                     /locus_tag="KPG13_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0983926.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(46298..46876)
                     /locus_tag="KPG13_000178"
     CDS             complement(46298..46876)
                     /locus_tag="KPG13_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0983927.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            47102..48025
                     /locus_tag="KPG13_000179"
     CDS             47102..48025
                     /locus_tag="KPG13_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0983928.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(48197..49339)
                     /locus_tag="KPG13_000180"
     CDS             complement(48197..49339)
                     /locus_tag="KPG13_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0983929.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(49357..51111)
                     /locus_tag="KPG13_000181"
     CDS             complement(49357..51111)
                     /locus_tag="KPG13_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG0983930.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            51247..51453
                     /locus_tag="KPG13_000182"
     CDS             51247..51453
                     /locus_tag="KPG13_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983931.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(51458..52381)
                     /locus_tag="KPG13_000183"
     CDS             complement(51458..52381)
                     /locus_tag="KPG13_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983932.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(52635..54335)
                     /locus_tag="KPG13_000184"
     CDS             complement(52635..54335)
                     /locus_tag="KPG13_000184"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG0983933.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(54797..55210)
                     /locus_tag="KPG13_000185"
     CDS             complement(54797..55210)
                     /locus_tag="KPG13_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0983934.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(55278..57656)
                     /locus_tag="KPG13_000186"
     CDS             complement(55278..57656)
                     /locus_tag="KPG13_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0983935.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            57975..59426
                     /locus_tag="KPG13_000187"
     CDS             57975..59426
                     /locus_tag="KPG13_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG0983936.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(59630..62143)
                     /locus_tag="KPG13_000188"
                     /pseudo
     CDS             complement(59630..62143)
                     /locus_tag="KPG13_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            complement(62366..63187)
                     /locus_tag="KPG13_000189"
     CDS             complement(62366..63187)
                     /locus_tag="KPG13_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0983937.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(63705..64181)
                     /locus_tag="KPG13_000190"
     CDS             complement(63705..64181)
                     /locus_tag="KPG13_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG0983938.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(64215..64793)
                     /locus_tag="KPG13_000191"
     CDS             complement(64215..64793)
                     /locus_tag="KPG13_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0983939.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(64897..67479)
                     /locus_tag="KPG13_000192"
     CDS             complement(64897..67479)
                     /locus_tag="KPG13_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0983940.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
BASE COUNT        21028 a        12636 c         7420 g        26486 t
ORIGIN      
        1 ataaaataca atatatgata aaataatata ttatataaaa taaatatatt atattataca
       61 tatacttatt tttactataa aagttaaatt tataagctta cttgttataa aaataacttt
      121 tataatttat ttcaaaaaaa atattgattt ttgtgtcgca ttttgttata ataaattaag
      181 gttatccccc tggtaaccct agttagttaa tatatctatt cccaataccc tttcataagg
      241 atatatttta tatttattaa aaatcggatt agtaacggaa attaattcgg tggaaaaaaa
      301 gagcttaccc cgagctcttt tttttctgca aaaaatcgta aaaataaagt taatattaat
      361 ataactttaa ttttaaaatt aatattaacc atcactaaaa aaggatgtat tgaatttcta
      421 atatcaataa catccttttt atatattata gcacttttgc taaaaatgct tttgttcttt
      481 catgttttgg atttccgaaa acttcttctg gagtattatc ttcaagtata cttcctccat
      541 ctataaaaat aactctatct gcaacttcct tagcaaatcc catctcatga gttacaattg
      601 ccattgtcat accttcttta gcaagttctt ttataacatc caaaacttcg tttaccattt
      661 ctgggtcaag agcagatgtt ggctcatcaa acaacatcat atctggttcc atagcaagtg
      721 ctctagctat tgctattctt tgcttttgtc ctccagataa ttgtgatgga tatgaatctt
      781 ttttatctaa aagaccaact ctactcaaca aaatttctgc ttttttttct gcttcttcct
      841 tagtcatctt ttttaattta attggtgcta atgttatatt atctaaaata gtcttatgtg
      901 ggaataaatt aaagttctga aacaccatac ctatattttg tctaacctca tcaatattag
      961 tttttttatc gaccaaattt tttccttcaa atattatttc tccacctgta ggtatttcta
     1021 gtaaattcat acatctaaga agtgtacttt taccagaacc acttggacct actataacca
     1081 ttatttcgcc tttagcgatt tctaagtcta tattttttaa tacatttaaa tctccaaaag
     1141 atttacttaa attttttatt gtaatcatat tatcactttc ctttttacta ttctatgttt
     1201 tccctaaatg ctaaacatgc tctcatctag ttagacacag atagtttctt ttctaataat
     1261 ccaactaatc ttgatagagt aaatgttata acaaagtaaa ttgctgctgc tactattaat
     1321 ggctctaatg ctctaaatgc tgcatttttt actatatcag cagtacgcat tatctcaaca
     1381 actcctatta cagatattat tgaagattct tttacaagtg taataaactc atttgctaat
     1441 gaaggtaata tattttttat agcttgaggt attattatat atctcatagt tgaccaataa
     1501 tttaatccta atgatctact tgcttccatt tgtccattat caactgattg tatacctgat
     1561 ctaagtatct ctgccacgta tgcaccagag tttactgata atgcaaatgc actagctata
     1621 aattcacttg gtataccaaa caacataggg aatcttattc ctaattgagg taaaccaaac
     1681 catactatat atatctgtac taataatggt gtccctctta aaacctcaat atagattgat
     1741 gatattgctc ttaatacctt gctctttgat atttttgcca tacagataat tatacctatt
     1801 ataaatccaa agcataatgc cacaagagaa attgatactg tcacacctgt tccttccaaa
     1861 aaagaagtcc caaatctact taaaaaacta aaatctaaac tcataatttc atctcccaca
     1921 taactttctt actaatttga agcagcttca gtagatgctt catttaaaaa ttcttctatc
     1981 ttcttgccat cttgtaattc ttttatcttt ttgtttaata attctataaa atctttgtta
     2041 ttgtcagatt ttttgattgc tgctgccata ccttctgtta catctttccc aacttcagta
     2101 tttgccattt ttattccatc atatttttta acatttatta aagaaactgc ttcattagta
     2161 acaactgcat ctatgtttcc attttttaat tctgtaatta aatcaggtat tgctttcaaa
     2221 gattttgtag ttgtcatttt taaagtgtta actacatatt cttcttggat acttcctgct
     2281 tgaactccaa ctctaagttt ctttaaatca tcttcagttt ttactttgtc tatatctgca
     2341 tctttaacta taactgcatt tctacttaaa taatataatt ctgaaaaatc aacactcttt
     2401 tctctctctg gtgttggaga catacctgca agaacaatat caacttggtc tgcatttaaa
     2461 gcacctatta atccatcaaa tgacatatcc ttaatttcaa cctttacacc aagctctttt
     2521 gcaaattctt cagccaacat tatatcgaat ccttttattg aatcttttcc atctactacc
     2581 ttatgaaatt caaatggagg aaattctgca ctagtaccaa caactaattt tccattgtct
     2641 tttatttgtt ctaactttgt tttttctcct ccaccactac atcctactag cgataatgtc
     2701 aatcctaata ctaatcctaa acttaataat tttttaaata ctttcatttt aactacccct
     2761 ctcttttaat tataatttcc ctcagatttt aatacttaaa tgtttcgagt tacaaaaaaa
     2821 gtctctcagt ctttaatgac taagagacga ctttaccgcg ttaccactct tattgataaa
     2881 tacttatatt actttcaaaa tttgtgactg ttagcattta agctgtatgg atacttttat
     2941 ttttaaatca aaaaagtccc ttagctttac agctaagaga cgaattatat ccgcgttacc
     3001 actctttttg ataagtaagt acttatcctc tcaatccctt aaggcgggta aaccaattat
     3061 catactaaag tttctgataa tctcctccag agctctcttc acatagactt cattgctagg
     3121 cttacaccaa cccctaactc gctgtaaatc catatatgct actttctcta tcattggatt
     3181 tgtttcctta aattgttaat acaattgtac ttccttataa tatttttgtc aataagtttt
     3241 ttattttttt aataattttt acttgtaaat tatctaaata ctactgttta tatttattga
     3301 tattagtttt gtaattataa atagtatatt tcatattttt agatatttct taaataatac
     3361 taaataatat ctttcacatt gttgcttttt cttaaacaat actaaataat atctttcaca
     3421 ttgttgcttt tcttaaatta cactaaataa tatctttcac actgttgctt tttctttaat
     3481 aatactaaat agtatctttc acactgttgc cctttcttta ataatactaa ataatatctt
     3541 tcacattgtt gctttttctt aaattacact aaataatatc tttcacactg ttgctttttc
     3601 tttaataata ctaaatagta tctttcacac tgtttctctt tctttaataa tactaaataa
     3661 tatctttcac attgttgctt tttcttaaat tacactaaat aatatctttc acattgttgc
     3721 ttttctttaa taatactaaa taatattttc cacattgttg ctttttctaa acaactataa
     3781 ataatatatt aaacccttat ttctttttct aataagttaa ataatacatt ttacacatat
     3841 ctatttgtct tgcacataat aaataataca ttatatttct atctaaattt agattctaaa
     3901 ttaaatagta taaattatat tcttcaataa acaagttttc aaatccaaga ttcgctctct
     3961 atctttaaac tttattgtaa acataattcc atctttttta attatttctc cttcatgaaa
     4021 ttttttatgg tataccttat cccctacacc aatactgtca atttcttttt tcgttggaga
     4081 ttttatatca ttaataaata atgatttatc aactttctta ccatacttat ttaaagtaga
     4141 gcttatacat aacttttctt cagctcttgt aatagcaaca tacataagtc ttctttcttc
     4201 ttctaattgt tcttcttttt tctcttcctt acaaatatca tatgattttt catgaggtat
     4261 tgttccttca tttacaccta taatataaac atttctaaat tctaatcctt ttgcactatg
     4321 catagttgta aatatgacac cctcagtctg cttattcttg ttattttccg ttaactcagt
     4381 ctttactcta tcaatatgct ctaaaaactc tgtaacagta ttaaaattag ttgctgaact
     4441 ctcaagttca tttaatatct ctacaagtcc attagtcttt atctttctgt tgctacaata
     4501 atctaaaaca tatctatcat aatcaagact agttctaata taagatattg catttttagg
     4561 atttaaagta tttaaataac ttaaatctat ttccaaatca tttattgttt taacttgttt
     4621 aggatgaaga ttacacttat ttattaatgc tgttataaag tctttttcat ccttaaccat
     4681 atttatactg tctttggata tatatctaaa aggcttgttt ataattctca accaatcttt
     4741 atttgatttt ggattaatac ctattcttaa ataggctagt atgtcttgac ttgcccaatg
     4801 gtcatatatt gtaattactg agtcttttac tacaaatgga atcctcatat ccataaatac
     4861 atctactaat gctcttgact gtatattagt tctataaatt actgcaaaat ctgaatactc
     4921 tacataatct ttttgtattt cgtcaattat ttccctggca atatataagg cttcttcctc
     4981 tgaatcatgc ggagaaatat aggtaacact tcctccatcc ccctgactac acttaatgat
     5041 tttttcatat ctatttttat ttttgtcgat aagtctattt gcagtatgaa ctatctcact
     5101 tttagacctg taatttatat ctaatattat ttttttagta ttattaaagt attgttcaaa
     5161 ttcaagcaaa aaatctggtc tagcacctct aaatccatat atactttggt cttcatctcc
     5221 aactgcaaat atattgttta atgggctaca aattaacttt aaaacttcaa actgtacttt
     5281 atttatatct tgaaattcat ctatcaatat atatttatac acatttctaa ccatttctag
     5341 tgcagactta ttgttcaaaa gtaaataata agttcttata agcatatcat caaaatctat
     5401 tttattaact ttagattttt gttcttcgta taagttgtat acttttaaaa actcatcttt
     5461 tgttaatact tctgaattaa attcattttt atccataagt tcatttttta cataggaaat
     5521 ttcatttata acttgtccta tattctcatc atcatcagca ttctctatat ttaaactttt
     5581 taggattgcc ttgattgtca ttcgctttga cttttcatca aatatactgt ctaagttgta
     5641 tctttcaaag tatcttagaa ttttaaaaaa tacagagtga aaagtaccat aggttacctt
     5701 attaagtctt atgtcatcac ttaaattcaa agctctattt ttcatttcta ttgaagatgc
     5761 cttggtaaaa cttattgcca agattcttgt tggtgctatg tttttgttta caaccatatt
     5821 ggctattcta tatgtaatta ctctggtctt acctgaacca ggccctgcaa gtatcatgca
     5881 aggcccatct atatgctcaa ctgcttctaa ttgattttca tttaactttc ttatatctat
     5941 atttgttgtg attattagct ccaccacctt gatttataat tatattattt tctttttaat
     6001 tataaactaa aagttcataa aaataaattt aatttttcct ctattaaacc acatcattcc
     6061 tattttcatg acatatcttg agttcttttt taattttgtc gcttatatct tcaggtataa
     6121 atgatggttt actaatatag tagccttgca tataatctac tccaagatta attagtgttt
     6181 ccatttcacc ttttgtttct acaccttctg ctatcacttt aatatttctt tcgttagaat
     6241 atgagataag attttttaat attttctgtc tgttttcatc tttatcaata cctcttacaa
     6301 tagacatatc aatctttaca tagtctggca ttatatataa tagtacagca tctccgttgt
     6361 aacctgtccc aaagtcatct aaagcaattt ctatattcca atggtctata tatgctttct
     6421 ttttctcaat aaaatcttca ttaatcttat cattctctgt tatttctagt actactctat
     6481 ttaaatattc accatataaa tcttcaattt cctcaaaatc tacttttgac aaattattat
     6541 taggaataga atttaagaac agtttgcaat cgccaaattt ttctttgtat tttttaaatg
     6601 atttaagtgc tttaaaccat gtaatacgct caatttgata taatttagac tgtgaacgag
     6661 ctaatctaat gacatctatt ggcgaactaa aagtttttat tttaggtctc ataagtgctt
     6721 catatgcaaa aactttacct ttacaatcta caataggctg aaatgcatac tcaactaatt
     6781 gaccatcaat taacttgttt aattcttctc tattactcaa taagaatgaa tccttattat
     6841 atatattaat atcaaattca ctaacttcac ccttgattgt atttttaatt tgatacatag
     6901 caaaatctgc gtatctcatt aattcatcat agttggttga gtcatcaggg taccaggaaa
     6961 ttcctgctga tgcccttatt ttaaattctg ttccatcatg aagttttaat aaggtattat
     7021 tcatctgttt cttaacagaa ttaataatat ctcttatact atctttactt tcatatccat
     7081 atataaatac ataaaactca tcacctgaaa gtctagatac tataccattc catgatataa
     7141 acttcttaag tatgtctgca gcacacctaa tatattcatc accagcatca tgtccatatg
     7201 tatcattaat atattttaga ttatctaaat cccacataat aaatgctgct atcttaagtt
     7261 catctttatt cttaaatttt tcttgtatct gtgaatgaaa agctctccta tttaaaagat
     7321 tcgttaaaat atcaaaatct ctttcatatt ctatcttcct tttctcaata aattcttttg
     7381 ttacatcaac aacaacacca agaaccttta gctcatcttc aaagatattc attcttatcc
     7441 acttaagttt tttatcttta gattcaaact tatatatata tgtattttct gtttcataac
     7501 tttcttgcaa atatttatct agtcccctta attttttcag gaacttattt ttttccatat
     7561 atccatattc ttcaaatttg atttcagtag taaacacgct aaaaaattct tttgtacaaa
     7621 aaacattctc tgcgtttttg tataactcaa aagctccaat tggcatatct accatttcaa
     7681 taatctgtga taattttgac gaagaatctg caacatttgc actgagcaac tctatagctg
     7741 aagataattc atctatttca gatatattta tcttatcaag atttactggt tttgatggat
     7801 tactgtttct aactttgttg gctaaaagaa caattggttt tgtaaataat cttgatgcaa
     7861 tataaatacc tattagacct attaataaag aaattgctat agatattata actaatttct
     7921 ctaccctttt tgaaaatgac aaaagacttt tttcttcaat aatacccaaa agagcccaac
     7981 gttcattttc aaatggagta ttggagttat ataaatctaa gtattgtaca ctagcataaa
     8041 tgttgccatt accacattca ttatctttat ttttaatctt atatatattt ttatacgatt
     8101 ccttctgctc taaattagaa tgtaaaccat cccaaaattt ttcttttaat gagtatccat
     8161 ttgaaattac attttcaaag gccatatcat tatttctatc tacacctaaa atataattac
     8221 cctttttatt cccatttatc tctgagtatg gaagtaattc tcttaaataa tttacactta
     8281 aatctattcc aagtactcca tatacagttc catcttcata aataagggga actgtatatg
     8341 tcataagcct atcacctgca tttgtcaata aaaacttact tccccagtat cctaaattag
     8401 aacttgatac atttggatta tcaattgcag ctctaaatgg tttataaaaa aaatcatctc
     8461 catcttgctc attttcactt ttaaatgaaa acttaggttc ccaataactg tccatagata
     8521 ttccaaaact tcttgctata tctgatgaac ctctctcaat taacaaatca gaattatcat
     8581 ttggattaaa cttagggtct aaatccctta tatacaatcc tgtcctactt atttcacctt
     8641 tactatctat tgacttatcg ttaatatttt tgtcacttag gattataaaa gctcctgtaa
     8701 cagaattttt tctcaaaaga tatattaaat catttgatat gctttttata atatctttgt
     8761 taatttcttc attgttgcct acatcctcaa ttaaggcatt tttttcattt aatacttttt
     8821 ttacagagga attaattgta gcttccgttt cacttgtatt tgaccatctt tgtaacattt
     8881 cattttgtat ataattttta cgattaatta ctctttcatt taagatgtca aacgaattat
     8941 catttaattt cttaatagtt cctccccata aaattgtggc acaaaataaa cttgtttgaa
     9001 ttagcataac aataattagg ggaaatatca tctttttcat tattgaattg ttttttctta
     9061 gattgttttc catacccatt ccacctatac ctttgtgctc gtcaacacac taactgccaa
     9121 ttcatctatt tttttatagc attttcaagc tcagccttaa aactttcaaa ccaagtttta
     9181 aaatttttat cattattaaa ttgtgctact gcatccttct tagaagttcc actttttact
     9241 aattcattta ttttatttga atcttcttta gctttatcaa gcattgaata ttcaagtatt
     9301 tgtctagctt tagttccacc atcaaatgct ttattagtat ataaattaca actgttaatc
     9361 tgttcaatag atgaggctat tgaaccttca actttatcag acaaactcaa attatctttc
     9421 ttcataactt cttttataac atccaaatca ttcgtttttt tctttactgg taagtatcca
     9481 gaagcaatag agaattttat attaggttct ttatctgtaa accattttaa aaattctact
     9541 gatgcatatt cttcttcttt agtagatttt gttacagcca ttcctgcacc ttgttgtaca
     9601 gcatattttc cttctccttt aaaacgtggt gcttgaaaaa cagatgtttc aatattataa
     9661 ctctcattat catttagcat tactctatct ggaaagtatg ctgctcctga actagaacct
     9721 acaagagcta atatatcacc tgtttttgca tcatcagaac gaaacttacc ataagaagca
     9781 aaatatccat ttatatatgg tacatagaaa ttatcccata gttttttcat aactttttca
     9841 tcaatattta gcgtaacttt accatcttta acagaaaaaa tttctactcc tagttgttta
     9901 cttcctataa taatataatt tgctaaagca tctcttccaa aaaatgcttt accatcatta
     9961 ggctctttcg ttaaactatc tgtccattca taatattttt ttgatgtctc tactaatcct
    10021 tcaattgttt ttatatcatt tatatttgaa tttgtagctt ttgcaaattt atcccaatca
    10081 gttttattta acatcaaaat ttcagttgat tttgcaattg ggaaaagttt aaactcatcc
    10141 ttagaattta ttctgccttc ttctacatat tcgtctatgt attcatttaa ttcttcttta
    10201 cttatgtact tatccatatc tactagtaac cctaatttat ccatttcata tgcagtatct
    10261 gagtatgctg caaatatatt tggtacatct tcagaaccaa cttttttatt tgcagaatct
    10321 aaaactttct ctgataaatc aactacatta ccttgactga atgcttcaac aataattcct
    10381 ttttcagcac ctacagtgtc attaaattcc ttaacaagct tatcaaaagc cattttctgc
    10441 tgtccattat aataatgcca aatttgtatt gatgtcggat tttctgggtc taacttataa
    10501 ttatcttcct tctttaaact acatcctgta agtaataaaa caatcattga tgtgataatt
    10561 aattttgcaa acttacacct attcataaaa tctactcctt caaattataa aatttataat
    10621 agcattaaaa ctgtaacttc actatttata ttaattatag aacgtttatt gatatattta
    10681 aacaggattt acaacatatt taaaagcaat taagaaacat actattacaa atatagaaaa
    10741 taaagctttt acataatttt tatttctaaa tgcaccaaat gacaccaatc cttgttgaat
    10801 agctactgta attactaaaa gtatcattat tatattaagt tgaaccagtt taaataagta
    10861 aagcaatgaa aacactacta gaagtacaca taatacaata ctagatttat taataatatc
    10921 atcaatctta tctactttct tagattgctt attaatttta tttaattttt ctttatttaa
    10981 attctcaact tttttattat acctttctct tgttttatct ttatatttag tatttttatt
    11041 tttatttccc atttattttc catcctctat aaatttttta attgagctta tacattattt
    11101 aaatattata tatttaagat accatatatt cctacaaaaa ggtaacttac tatgtatcta
    11161 tttttgattt tcgcataaaa aagccatctc ctttagaatt aattctgtat tgagacagct
    11221 aatttaacta tttatccttt gaaacatttt ctacattatc atatttacta tcaactaaaa
    11281 ctatatcaac tcttctattt tttgacctac cttcttcaga tgaattttct gctattggtc
    11341 taaactcacc atatcctaca gcagataatt tatctggtgc tatacctgca ttgtcaataa
    11401 gaagttgaac aacattggtt gctctcataa ctgataaatc ccaatttgac ttaaactctg
    11461 aattttttat agacatattg tctgtatggc cttctactct catgtaacta ttcatttcat
    11521 ttaacatctt acctatttga ataatcttat ctctagaatt atccttcaca atagctttcc
    11581 ctgtatcaaa tagtattgta tctttaaggc ttattactaa tcctctatct tgaactctta
    11641 atgatactga attagccata ccattttcgt ttaaatactt ctgaatttct tcaccaattt
    11701 ttttaaactt agcattttgt aaagtatcta aatcattact tcctggttca actactggtt
    11761 ctatcttgct attaccacct tcaataactc cagaagaacc accaaaagca gaatttagag
    11821 attgagataa ttgtttatat ttctccgcat caacattact catagaatac attattacaa
    11881 agaaaatcat aagtaatgtt atgaggtctg aataagttaa aagccatcgc tcattgtttt
    11941 cttctttttc atcttcatca tacaacataa acattccatc cttttatatt attcagctct
    12001 accaccaatt tcttcaagtt caattagttc ttttgaattt aaaaatattt ttaatttttc
    12061 tgtcattaaa ttagaattat caccctcaag tatggataat attgcttcta tcaccaaaag
    12121 tctttcactt acttcttgtt gatttatagc cttaagtttt gatgctattg gtaaccatat
    12181 taaattggca gaaccaacac catacaaagt agccaagaat gcaacagaaa tttgaggtcc
    12241 taatgcatct ggattacttg aaagattacc taaaatatgt actaatccca taactgtacc
    12301 tattatcccc agtgtaggtg catatccacc tgctgaatca aaaattgcta ttccttcttt
    12361 atgtcgtttt gatgttatct gtatctgtga ttccaatata tttcttatag taactgcatc
    12421 cactccatct accataagtt caagcccttt tctaataaaa gggtctaatt catcattctc
    12481 acttatttct ttgtcaatac taagaagccc ctctttccta gcctttgttg caatttcttt
    12541 gaaatagatt atattttcta tgttattagg ctttttattt ttaaaagcta ataacattat
    12601 ctttatagac tttttaaaca cagggaatgg agtcgaagct ccaacagctc ctatagttcc
    12661 accaaacaca acaagtgctg atgaaaatac aaataatgct ccaacatgtc ccccatcaat
    12721 aacaaaagca actaccaaag atgctatccc cactatgaaa aaaattaaag ttgtcatcta
    12781 ttccctcctt tctttagtta tacatatcta atcatttatg acattataaa aagactatct
    12841 ttaataaaaa tagcctttaa taattggcgg agaaggaggg attcgaaccc tcgcaccggt
    12901 tacccagcct aatcccttag caggggatcc tcttgagcca cttgagtact tctccatatc
    12961 tctatactaa taaattatat aataaaatca tattttagtc aattgtttta taaaatttga
    13021 gttattttga attattaaac atctattttt acttttttgc ttaaatgtaa gttttaaata
    13081 tggtaaaatt atcttgcata acacttataa tattatttga taaatataca tacattaaca
    13141 aatataccta aactctgact taattgtaag ttatgccaaa actaaaataa tactagtcca
    13201 aggaggatat tttatgttac cacaggaaag atatgagaag attttaagta ttctcaaatc
    13261 agataatata attaaaataa atgacatagt tcaaatgttt gatatatcaa tagaaactgc
    13321 acgaagagat ttagcccata tggagagtat cggtctttta aaaagagttt atggtggtgc
    13381 tataccagtt atagcaagta gcgtagaact taactatgtt aaaagaaaaa aaataaactc
    13441 aaacgataaa aaacagattg ctattaaatg tagtgagttt ataaatacaa atgatactat
    13501 ttttatggat actggaacta ctgtacttga agtttcaaaa aatttgaaag ataaaaagaa
    13561 tctaactgta ataaccaact caattttagt tgcaaacgaa cttatgaata ctaatataaa
    13621 agtatatgtg cttgggggct atcttagaga aactgaaggt tctttttctg gtccgcttac
    13681 actgtcagct attgaacaat ttaatgttga taaagctatt ttaggcgctg gaggtatcac
    13741 tcttgaaaat ggaatctctg actatcactt agaagaagca cttgttagaa aaaaaatgat
    13801 tgaaaggtct agaaaatcta tagtagttgt cgaacctcct aagtttggta taaattcatt
    13861 tgcatcagta gctcctatag aacatataga tactattatt acctctgcta gtatagacca
    13921 taatataata atagagtttt ctcaaaaagg aataccttta gtcatagctg ataattagct
    13981 tatatgattg atttttagtt ccttgatgta ataaattaaa attaaggagc caaataatta
    14041 tttatttagc tccatttatg tctaaactat actttatatt ttaattatgt aactactttc
    14101 agttagctta tcatttttag ttatttgatt gccattttat ttaatttata agttttagtt
    14161 attcgattat tttttaattg tctgtctttt taatttattt ttagttattt aaactcctac
    14221 tttaaaataa tttaaatttg acttaagtat tgtttattct acactgcatc tttcttcatt
    14281 ttaatacttt gttcttcttc caaggttaaa tcttttgtca taagtatagc aagtgcaatt
    14341 gccaatactc ctccaaccaa ttttccaatt acaacaggtg taatcatctc tggaactact
    14401 cctgctgtaa atcctaaatg gtcccccaaa gctgctgtag cacaaaccag ccaagcagta
    14461 tttactactt tcccctttgg actcatatct ttcatcattt tatatactgg aatactattg
    14521 gctaaagtaa aaactaatcc tgctgcacta gtggcatcca tacctaattt tccacccact
    14581 attgtaagtg gtttattaag aactttaact aataaatgta atattggaaa agtacctaat
    14641 aacacgattg caatacttcc aattacttga ataccttctc ttataggtgt catgcctttt
    14701 ataagtacta tacctgttat ttcttgaaat gctgctgctc ccaaacctat cgttatcaat
    14761 atagatataa attttccaaa aattaatgac ccatttatca taagagtaga attaaatttt
    14821 aaacctactg ccaatattac tgccaatatt atcactggta tcatatttat aattaccatt
    14881 gataagttaa atcctgctat taatcctcct ataatacctc caaagggtat tgttatcaag
    14941 cctatcagta acccttttgc aaagaatggt ctgtcttcat attctataag acctagtcct
    15001 actggaattg aaaatactaa tgtacatcca agcatggatg acacaaccaa tcctgcaagc
    15061 attcctgcct ctttattgtg tgcaagttcc atagccaatg gataccctcc catatcattc
    15121 gctaaaatag ttgcaaacat agatgggtca gctccacaga gattgaagat tggtattatt
    15181 ataggaccta gtacatctga aattactgga gctaaacaaa ctataccaac cattcctaaa
    15241 gccaatggac ccattgaatt aaacccttct tcaaactgct cgccaagtcc aaatttattt
    15301 ccaattattc tatctaacgc tcctaaaagc actcccacag ccattatgta taatataatt
    15361 ttatcaaagg gcatttttaa ttcctcctat acatgtaaaa tactctattg taacttaatt
    15421 agcacacacc tttttggtat cgttttccca attcatagct ctttttactg cttggttcca
    15481 tccaagtaat atttcttctc ttttttgagt agtcatattt ggaatatatt ttttgcttat
    15541 tgtaaattca cttctaattt catcaatact gttccagaca ccacaagaaa gacctgccat
    15601 atatcctact ccaagtactg tcgtctcagg attttctgca atttcaattg gtatacctaa
    15661 tatgtcggat tgaaattgca ttgtaaagtt atttttagta attccaccat ctactctcaa
    15721 catttttatt tttttccctg aggctaagtt catagaatca acaacatctt tgacttgaag
    15781 tgcaacagac tcaagagtcg ctcttactat atgctctttt ttagtacttc ctgttatccc
    15841 aaccattgta cctctagcat attggtccca ataaggtgct gctatacctg caaatgctgg
    15901 tacaaaatat acacctccag tacttgatat ggaatttgcc atgtcatcag tttctgaata
    15961 attttttata actccaatgc catctctaag ccattgtatt gcagcacctg ctatatacac
    16021 tccaccatca taagcatatg taactttatc tcctattttc caagcaacag ttgtcaaaat
    16081 accattacct gctatattta gttcctcacc tatattcata tataggaaac atcctgtacc
    16141 ataagtattt ttaaccattc ctggttcaaa acatccattg ccaaataaag ctgctggttg
    16201 gtctaccatt gaagcagtaa tttttataga aaccccacaa aatgcctcct tatctgtagt
    16261 tccaaaatca ccactcgtag gcattatatc tgctaaaata cttctaggta ctcctataat
    16321 gtcaagcaac tctctatccc aatccatatc tcttatatta aaaagcattg ttcttgctgc
    16381 tgtagataca tcagttaaaa aagattttcc tcctgtcatt ttccatatta accaactatc
    16441 taatgttcct gcaagtactt ctccattttt aattttttct tttacacctt ttacattatc
    16501 tattacccat ttaatttttg tacctgagaa atatgggtct ataattaacc cagtcttatc
    16561 tattatctta ttttctaaac ctggtatatt ctttaaattt tccacataat cataagttct
    16621 cctgcactgc cacacaatag cattgtaaag tggtttacct gtatttttat cccataacat
    16681 tactgtttca ccttgattat ctataccaat tccttttata tcactttcat tgattccatt
    16741 ttcaaaagcc tgttgtaata cattattact cacagttttt gtattattcc aaatttctat
    16801 agggtcatgt tcaacatatc cagattgtgg atatatttga gtatgttcca tataatcgct
    16861 aaaaacaaac tctgattttt cgtttatcaa tactgctctt gttcctgttg ttccttggtc
    16921 aattgctaat acatatttca ctttaaatgt cctcctttat tttcagaatt aataaattct
    16981 tttttatttt ttgaatatat aatctattca agttatttat attttatatc atcatgtgga
    17041 tttatgcaat attcattaca atattttttg ttatttttaa ttatagtgat tgaaaaagta
    17101 tttttattga aatattgttg caatatttat tattttattc tataatctag ttaaaataac
    17161 acataatatt ttaatattta cacataatca gtcattcaaa aatttaaatt taacttttta
    17221 aggaggcttt acaatgaaaa aaacatatct attcagtcct ggaccagtaa tggttacaga
    17281 taaagtcaga aattcacttc ttcactatga tatatgccat agaggaaatg agtttatgga
    17341 tttattcaaa gatacacaac aaaaaatcaa taaactatat aatgcaactt ctaattacta
    17401 ctcattaatt gtatctggct ctggaacatc tgtaaatgaa tgtgtactat cttctatctt
    17461 tgacaaagaa gatgcagttc tgctagttag taatggtgtt tttggagaaa ggctagaaga
    17521 aatattatct acttataatg taaaaacata taaacctaat tttgaatggg cagaataccc
    17581 ggacctagac attttagaaa aatacataat tgaaaactct gatataaaag taattgctat
    17641 ggtgttccat gaaactagtt caggaatgat aaatccagtc cctgaactag gaaaacttgc
    17701 taagaagtat aacaaaatat tttttgtaga tgcaataagt gcctcagcag gtgaatatat
    17761 tgatgtggaa gagtttaata tagatataat aacaggtgtt ggtggcaaag ctcttggagc
    17821 atttccaggt tctgcatacc tttgtgctaa agagagtatt ttacaaaata ttaaagaaaa
    17881 tcaatgtaag aatgtgtatc ttaatcttta taaacactat aaagttgcta aatcatcttc
    17941 tcaaacacca aatacaccta atgttacttt aatatttgcc cttaatgaag ctttaactga
    18001 gtttttggaa gatgacagta aaatagaaag atacaaagaa tgttctgcta ttttaaggta
    18061 tggtatggaa gaattgggtc taacattttt acttccagat aagtatatgt ctaatacagt
    18121 aacatctgta tttctaccta aagaaatcaa tgtaaatgag tttatattag ggctagaaag
    18181 ggaaagtggc tatgttgtct atccaggaaa aggcaaattc ctagatgcta acatattcca
    18241 agtagcaaat atgggagaaa tatatccaga agattgttat aaattcttag atatcttaaa
    18301 atcaaaattg gagataaaaa aagttatata acagtttgtt tgtaaaatcc tacaatatat
    18361 cagtataaat taaatagact gattttttta aattttccta taataaatta tattttatat
    18421 ttttgtataa gggtgaacta taatatgtaa agttcacccc tatttcaaat ctttttcctt
    18481 caatataatc tttttctttt aatttatttt atatttttga cgcaatcgtt ttttattcaa
    18541 tcattttata ttcttgatac aatcattctt acacttaatt attttttgtt tttgacacaa
    18601 tcgttttttg ttgaatcatt ttatattctt gatacaatca ttcttacact taattatttt
    18661 ttgtttttga cacaatcgtt ttttattcag tcatttaata ttttcaacac aatcattctt
    18721 acattctatt attgtttgtt tttgatacaa ctgtttttat tcagtcattt aatattctca
    18781 acacaattac cttttcattc aatcaaagta atctttaagc attatttttc aagctcatca
    18841 aagtaattat atacgataac ctcacctgtc tctttatctg tagtctttat aatgccctta
    18901 gctccattta cctcttcttt tgtaaattca tcatctaaca caactttgaa tttatcttcc
    18961 tttccataac cttgttgaaa ttcatctctc aaaattgcat ttttttctgc ttttggcttc
    19021 aactcaaatt caattccaga tttcatttca tcaaaataat ttaatactac aacctcatta
    19081 ttcattttat tagttgtctt tataatccct ttagctccat ttacttcttc ttttgtaaat
    19141 tcatcatcca atgttatatt aaatttgtct tcttttccat attcctcttg aaaatccctc
    19201 ttcatttcat tgtatttagt ttcaaactct ttactcttgt ttaattccat agctgataca
    19261 tttgtcaatg atgctccaat tgccattact gttaatgcca ttactatacc acttttaaat
    19321 ttttttactt tcatatctta cctcctaaat taaatgttct caatgaaatt atataaagaa
    19381 aatcctaagt tttacagttt cattcttgta actaaaatta ctattatgta accaaacttt
    19441 ttgcttcaac tattccttaa aagttgcttt tttgaggaat agttgaagtt aaataattag
    19501 attttatcta tatttataga tttaaaatca aatttatcca tataaataaa tacttatttt
    19561 tgcaaaataa aaatttttaa ttactccaat ccttcttatt tacttaaaat cctatgataa
    19621 gaaatttaaa agtctttacc ttctttaaca ataatttatt tacttttaac atacaaaata
    19681 tagagagtat atcaaaacta ttttactgaa tattctaaaa ataagtaccc cctattagta
    19741 tatatattta catattaaaa aaaagtttta aataaagtta tattttttac aacagttttg
    19801 taattaaaaa catattgaag gtaaatttaa aggtactata tactataaat atgtcgacac
    19861 aataaattta aaaaggagaa ctaaatatga aaaaaagatt aataataatg atattgtctg
    19921 ttgtcttagt actatcaagt atacttacga tatttgcata ttcaaatatt aaatataata
    19981 ataacaaatt gatatattct aatatgatag ataaaaaaac tcaaaatagt gtaaaggaaa
    20041 tcctaaaaga aaataaaatt aatgaaaaag acatagatac atttattaaa tcagtcaaca
    20101 attataataa attgcaagtt aaaatacttc aaaataatat aaatatatct aaatctggat
    20161 attcttcaat caatgcaaaa caagttccat ataacctaga aaaattgcaa gataattggg
    20221 tgaaaaaatt ccctgattat atggatgtaa attgtagaat aactgctttt agacttttta
    20281 aagattttat aaattcaaat aagaaattta ctggagactc tattgatttg aatgtcgact
    20341 tggacactat aatgaataat aaagatgcta aatttagcac caaagatgta gaaaaattta
    20401 tcaacttctt ttctgcaata ccagctaaag atacagatga tacaatcaaa attgcagaac
    20461 aaataaaaaa tgaatggaaa aaaagaaaaa tatcttttaa aaataataaa aatatatcta
    20521 ttataaatgg atttcttcgt tatccagaaa ctaaaaatgt atttataggt catacaggca
    20581 tatgtattaa aactaaaaat ggcattctat ttttagaaaa atacggtgtt acatcacctt
    20641 atcaagttac taaatttaaa aataaagaag atgtaaaaaa ttatatgttt aatagattga
    20701 agatgtcaga aggtgaaatt gaattacctg accctataat aatggaaaac gataaactta
    20761 tgaaataata actttaaacg caaaatagta tttataatta ctaagtcaat ataatagaga
    20821 aggtctatag atttgtgtaa atacaagctt atctacaatc ttatataaga atgtttatat
    20881 aaataagata gattaattct aagtcctatt attctattga attttattat tattttattt
    20941 agattctatt gttattttac ttagaactta ttattatttt gtttaagttc tattactatt
    21001 ttcaatcata taaatttaca aaagactgta cataaattta ttttatacaa acttatctac
    21061 agtcttttgt gtgaatatat ctaaataata tcgtatttat ttatttcctc tgcacagtga
    21121 gaatcagaac ctaaaactaa tggaatatcg tattttttta ccaagtctaa taagtatcca
    21181 tctatataag gctctttgca gtactctttt ctatttccag aaacattata atcaagttca
    21241 taaccttttt ctttaattaa ctttattaac tcttcaagaa ttttatttcc tttataatcg
    21301 tatggaaata cctgattaaa ctttctaact aaatttaaat gacctattct ttttggttta
    21361 tgtataccta aatctgaatt tacagctttt atcaatgttt cataatactt attataaaca
    21421 gcttcaatac ttcctaattt gtcgattaaa ttttgaaact cctctacact gtaatctaca
    21481 caataataat catcatcaat cttaattata tgtactgata aaagagaatc ctctaattcc
    21541 tttccatatt tatttaaatt ctcctttatc ccttcttcgt atccttctat aaaatccact
    21601 tccgacccaa catttatctt gattttaccc tcatactttt ctttcaactt cttgacatca
    21661 ttaaaatact ttggtaaatc ttcaatactc atacaactat cattttcagg agcagggtct
    21721 ctaaatccat ttgcatacgg aaaatgttct gtaaatgtca tttctgttat tccaacatcc
    21781 attgctcttt gtacatattt ttcaaaatca tccttacttc catgaggaca ataaggtgaa
    21841 tgtatatgac catctttcat aaataattca ctcctttttt aaagaaaaaa tctaatataa
    21901 atgcataaat cttttaactt caaaataata tttttaatca tgctaatttt tattatatct
    21961 aatattagag taattttaaa ctgcctttta agtatcttat cattagtact attttcaact
    22021 taacattaat attatttttt aacttaacat taatactatc tttaaatagt aatactcaat
    22081 atataattac ttaacataaa ataactcaaa ttgcttgatg aggcttttgc agtatttaaa
    22141 gaatatataa ttatttaaca ttaaataatc caatcttgta ataaaattta ctcattaaag
    22201 tatattacag aaaatacctc aaaataaact ttttagttca tgatatactc ttttttgcat
    22261 gaaattagat atatttttat catttcaaca atgaaaggga ggcttatatc catctttaga
    22321 caacccctta gataaaattt ttaataatta aacatcacaa aatataaaaa tcgcttatat
    22381 caaacgtata agcgattaat tatatttaca tatgtttttt agcctctaac aacattttat
    22441 tagcttcttc catatactct atagcctttt caatactctt tgctgtttct tcttttccaa
    22501 tagctctagc cttttcaacc cactctctaa atccttcttc atgagtttca ttgtgattta
    22561 tccaatgtac taaaagtatt tttaaagttt tttcatcctt agactctgat gattcctcat
    22621 gactatgttc gtggtcgtgg tcgtgagcat ggtcatgtgg atgttcatga catatatctc
    22681 catgagtatg ttcgtggcag tggtcatggt catgatgtcc atgatgatat cttacaatct
    22741 ctttatcaaa aatcattagt tattccccct gttattttat attaaactct ttttttataa
    22801 ggtttacaga atcttttaca agtagtgcta atggtttacc ttctaatcca actaactcca
    22861 cattttctgg cataataggt aaaagtatct taagtgcctc actgtctgca atagcttcac
    22921 ttatattaca agtaacttct cccatcatag aattaggtat tattacagaa ataggtgcaa
    22981 ctattatatt tgctttttta gctgatacta ctatagcatt ttcccctgta gctcctttat
    23041 tagcatgagc tttcatcata gaactagttg ctatagaatt agtccctagt gcataaattt
    23101 ctacatatgt aggtaactct tctctcaaag aagaaactat ctgggctcca atacctccac
    23161 ccattccatc tatcactgct attatcatat gaaattcccc tattaattaa ttttcttgta
    23221 ttattatctt atggtccatc aatcttattt ctttaattat cccatctatt atcttttgct
    23281 ctcctaataa atccgttaaa taaatctttc catcacaagg gtctatatta accacatttt
    23341 ccataacctt ttctaactca ttattacttt tacaaatgaa agctgatgat tcacacatat
    23401 aaatctcctc ctaatatgat ttttattaaa aatcaaaaaa agattccctt aggtatttca
    23461 atacaccaaa gaaaccttca tgatttcagt ttttgtatat tattatacct taagtatatc
    23521 actttaattt atgtaaagtc aattaaagtt aacgcttttt attatactct tcattcttgt
    23581 atttttcata tagttcttta actaattgct tctcataatc agtcaaatcg ccctccacaa
    23641 actcaacatt aaattgttca gctaatccct ttactatgtt tttctgtaga atatctatgt
    23701 ctatttttct gccaatttca ttttctattc cacttgcttt tttagctgta aatttcatag
    23761 ctctttcttt caattctggt gtcttagttt ttattaattt aaacaatttt tctacatcaa
    23821 aatccagtat tattgaacca tgttgtaata aaacaccatc ttttctactt tgagcacttc
    23881 caattacctt cttattatta atagtaactt catatgatgc atgtgcatta aaacatgcag
    23941 ctgataaatt ttccctactt attctctcgc ctctatttaa attatctgtt tctataccac
    24001 ttagattcaa ccctttaact agaccttcac ttatatatct ataagatagg ttgatgctct
    24061 tatcgattaa aggattatct tctcctataa ttatactata ggttagctca ttatcatgta
    24121 atacagctct tccacctgta attcttctag tatagtctat attcatacat ctacacttat
    24181 ctaaatctat ctcatcttcc aatttctgaa aatatcctat actcaaacaa gctggttccc
    24241 atgtataaaa tctaagagtt ggcttattat gtcctttctt atatgcagta aatatagctt
    24301 cgtcaattgc catattcatt gctccttcat atgatttatt atgtataact ctccactgat
    24361 tcatgattat tacgcctcca tttttagtat aaatatatcc tagaaatcct ctatagattt
    24421 atttctctta ttgctaaatc tatctcactt tgatttaaag gtcttgaata gttgtatata
    24481 gtacttcctg gtctttcatt atagtatggt ctattacaat ccttacaacc tcttatttca
    24541 aaaggatagc ctttgtttat gtcttttaaa atatcattgt caagctttat actatttaag
    24601 tatccatttt taaacttaaa atactctttt ggataacctt tatttatcat ataagacatt
    24661 aactgtaccc ttctataact ttctatgctt ggttgattta tcttttccat tttagttcct
    24721 ctaactggag taaatgcaaa taaactaatc gttacatcat tttcctttaa ataagtatat
    24781 aaattgtata tatcttcatg actttcaccc ataccaacta ttatatgtgt acttatttta
    24841 ttgggatatg attttgacat ttcagttata aattttaact tttcatcata gttatttcct
    24901 tttatttttt catatagttc tttatttgct gcatctattg ctattcctat tttatcaact
    24961 cctgctgaaa aaaacttctt tatctgttca tcattttcta attttgcaga tagtgaaatt
    25021 ggcatgtcta tcttaccact tatatagttt ataaagtcta aaacagagtt atgtgcttct
    25081 tcacttgcca ttgattgaat acatattctc tttatatttt ttcctttata agcttttaaa
    25141 gcatctaata tttcttcttt tgaaaactct ggccaaatta ctcttgataa tttatctttt
    25201 ctagtactac tttctattga ttgagaacaa aaactgcatt tatttatgca tttttctcca
    25261 atcattatat atgctgtggt aggaggtata tcgctttttt tgtttaatat cccaagttct
    25321 attgctgtcc caactgatag tctaatcata aaacgcaaca ctccttacac tcatttattg
    25381 tcatattcat ttctatagcc ttattctttg cagaccttga tggtaatact attctattga
    25441 ctccacacat tatagataat tggtctagct cttttctata cactcctctt ggtctcatac
    25501 aacctagatt aatttcagta tctggtaaat taattctagc ttcacataaa atatctgcta
    25561 ccccttctaa ctcaggaggt tctacatttt catactcagt cccttttgtt ggaataagga
    25621 ctataaatgt aagcttatta ggaggatttt tttgtaggta ttctataatt tcatattcac
    25681 cctttatttg tcctcctttt aaaccaatac atatatgtgg tgctacttct gtatgttctt
    25741 gtattgtatt atagacttca atataatctt ctttactctt tttcatctta taaacttctc
    25801 ttattgtctc atcatcaaac actaaatcaa aagaaactat atctaaatac ttacacaact
    25861 ctacaatgct atctttatcc ataagaccta aatgagcatt caatctatat ccttgctctt
    25921 ttaagttttt aatggtatta atatgagtat ttatagggac atctccctca taactacaac
    25981 cgccactcaa tagaaagcta gttatattcc ttgattgtac tttttcctca tattcttcta
    26041 ttggcaccat attgtttaag tagtgaccgt tgcaatgggc acatttcaaa ctacattgat
    26101 tgcttgtagt actaagagct agtgtctggt taggataagc aaactctatt ttatttccaa
    26161 aattctttaa tttaatatca aatgctattt tcattttttc agatagattc ttattcatag
    26221 tttattcctt cttaagtaca ttttactctt ctagtaattt ctttgctgtt gggaactgct
    26281 ctatattcgt atgtggtaag aaacatgcag aaataaattc atccatataa cttggatata
    26341 cactaagttc aacataagtt atttgattac ctatttcttc aagtttattt tttgcatctt
    26401 tacttataag cgctaagtaa gaaccaacaa gtgaactatt ccctatataa gtaaattttt
    26461 ctctatcaat atcaggaagt aaccctataa ttattgagtt ttctatgtct aggttgtttc
    26521 ctataccacc tgctatataa accctatcta atatactaaa atccatacct aaactatcta
    26581 caagagtgta tgcgcctgaa tatatagctc ctttagctct gatgaaatta tctatatcaa
    26641 cttcattgac tactatatca ttttctaaat caaattcttc tttaaatgct aatacatact
    26701 ctcctatctc atgctcatta aatctaactc tcttattatc taaatccctg tatattttac
    26761 ctcttctatc tattacacct ttagtaatca tttgacagat taaatcaatt ataccagaac
    26821 cacatatacc aactggtgca cattcatcaa taatctttaa tgttggctct aaagtatctt
    26881 tatcaataat tactttctct attgctcctg ctgatgctct cattccacag cttatccctc
    26941 caccttcaaa agcaggtcct gctgaacatg cacagctcat catataatct ttatttccaa
    27001 atacaatctc tccattagtt cctaaatcta taaataaaac attttcttca cttgaccata
    27061 tccctgctga taatacacct gctgttatat ctcctcctac ataacttgca acagatggag
    27121 ctaagtacac atatgcactg tcatttacaa aaagaccaac attttcaccc ataagttttg
    27181 gagattttaa aaatggtggt atgtaaggtt cttgtcttaa gaagtctgta tacacaccca
    27241 agaataaact tgtcatagtt gtatttccgg cagccactaa tgtaactaca tcttccttat
    27301 ctattccatt tctttcatat attgttttta atagtggatt tatagtttcc tcaactatag
    27361 ctttatgaag tgtctctaat ccatttttct tagttgaata tataattcta tttataacat
    27421 ctgctccata ttttatctgt gcatttccag aagatgcttt atctactacc tcttttgagt
    27481 ataggtctac taaacaaaca actactgaag ttgtaccaat atctattgca actccgtata
    27541 aggaattttc tttatttcct tgttctatat ttatgattgt aagtttcttt tgtttttgaa
    27601 catatgttat tgtaacctta aaatcactct ttctaaatac agttggcatt tttctaagta
    27661 tatccaatct aaaatctatt tcattatatc ctaggttatt tctaacatat ctttccaatc
    27721 tatctacatc acttatatta tcatctaaat ttggttcttc catttctatg tatttctttt
    27781 ttatatttgt tttaaattga aggttatgtt cttcaattat ttttttagct ctttcaaaaa
    27841 tttccctatc ctctttttta ttactacctt ctatcttcat accatgcata gaagatgata
    27901 ctttagaagg aacttcaatt tctatatctg atattacctt tgtacaacaa gctagtatat
    27961 aaccttgctc ccactcatca tctgttatat gtcttgtttt ttctgtatca acttttccat
    28021 tcaataattt tactttacac ttcccacaag acacgtttcc attacaagga gcatctatga
    28081 atatatctgc atttcttgca acctctagta atatatcccc ctcattacaa taaacctctt
    28141 tattgtttgg tgtaaaactt acttttatca tgttatccct cccaatttgt tctatatatg
    28201 ataaaaggaa agtatagcta aatttgcgtg cactcaaatt ttactatact ttccccaatt
    28261 ataataatat cttaatcttt aattattcta atccagcctc gtattcttta tcacttaata
    28321 acgtatcaag ttcagccaca tcagctagtt ttacttttaa tatccagttt tcatatgggt
    28381 cttcatttat acattctggc tcatcttcta atttttcatt aacttccaat acctcaccat
    28441 taacaggaga tattaaatca gaagctactt tagaagactc aactactcca aaatctactc
    28501 ccttagttac agtatcttct acttctggtg tttcaacaaa caatatttct cctaattgat
    28561 cttgagcata gtctgttata cctatatata caacatctcc atcaattact tttacccact
    28621 catgatcttt agagtatttt aattctggta ataatttcat ttcgatagac ccccaatatt
    28681 atattattga ttaaattact taaatcatat taatattata tcatattatt ttaaattttc
    28741 catcaattac attacacttt ccatagctaa tgctggatga cctttttctt ctaagaaagc
    28801 taatactcct tcagaatcgt cagctatagt ttcatcacaa accatatcac agaagttttc
    28861 tattccagtc atttctttaa ctgtagcatt taatttatca gctacataat cttttaattc
    28921 ttttggcatc caaactattc tttctggtcc accttctgca taagcgaatt ttttagaaga
    28981 tataaaatgt cttccgtgtc ccataaatcc tggagtttga actcctccac ctgtcataga
    29041 agcaagttct ccgaaagtca taccaactgg agtaacactt gcaaattctc tgttaactac
    29101 aacaaatcca tttgcttctg gcattatacc acagatacat tcgaagcatc cacaagaagt
    29161 cattgggtct tctagtatag agtataatgt aacagattca actgcacctt gagatatttg
    29221 gtttacagtt tcatttactg aattccatac acctgttcta tcatctaagc actcgccttt
    29281 ttctattggt tgacaaggac ctgttgggtc taattcttta gtagccttag catctaacca
    29341 gctaactgct ccacaaagac caagtctttc aggagtaaca acacaaacgt gagctggtgc
    29401 aaatgattga cataagttac aagaatagaa agtatcaaca ctttcatcaa ctaatgaagc
    29461 taatctttca tctctagcat tgtacttagc tattgcttct ccttttaatt cagaaacttt
    29521 ttcagcatct gtaattatag ttatttcaca cttatcaact actgattcaa attcatctaa
    29581 catcttagca tataaaactt ctcctatatg ctcaagtcta aatcctttat caaatgcatc
    29641 tttagagatt cttacccaag ccatatctct ttgtccaacg tgcattacac cttctatata
    29701 gtttaagaag tagtggaaac gtctttcaag aactggctcg aaatcttctt gcatattttt
    29761 accagctatt ttaactatta cagcaagtgg taatcttaat acaccatctg catcaacttc
    29821 atctatattt ggccctatta tttctatctt atggtcttca acttctgttg cttctttctt
    29881 aacaacaagc tcccaagctt cagttctgtt tccaccaaac tcagcaaaca tatcgctctt
    29941 tctaattctc tcaccttcaa atgctgctgc aaatgaaact gggataggta tttcagtaac
    30001 ttttattttt attcctctag cttctaatga agtagctact atcttatcgt aatctttttg
    30061 agttaatagg ttcattggaa cttcaagaac tgtttggtca gttataactg ggaatcctaa
    30121 agctattgct ccagcacctg cagatacaac taattcactt aatggtccaa atgcattaac
    30181 aaatgcagga actctattag ctgtgtactc taataatccg tttaaatcac caggagttaa
    30241 tccaccgaat attaatgctg ctcttactgc aacagataca acgtgtataa cagaagttac
    30301 atcatatcct agaggtataa ctcttagctc aagacccatt tttactccag cttctatagc
    30361 ttggtctata actttaccaa ctaagaaagt tagtaaacct ttagattggt aatcttttat
    30421 aacttttgct gctgattctg catctggaca ctcaccaaga acaactgcaa caccaggtat
    30481 atctcctgta actaatggta caccaagtga tctgattata gggtcagtta tatgacctgc
    30541 acatggctca ctatatggag catccatagt tgaatatttt aatgcttcaa ttacttctgc
    30601 tgctaaagct gtagctaaac cagcattaaa agcatgttct agtaaatgag ttctatttat
    30661 taaagatttt actacttcta aagcaccttc taagtcccct aaagtgttca tcttttgtcc
    30721 tgttgcagca tatatacaag gtaatgaata tgctgtatca gggaaagcaa ctttatgctc
    30781 ttttccattc ttttctatag cttcagctaa catagcttga gccgcaccta aagcttgttc
    30841 tgaccctgta aagattatat tatatagatt cattctattt ccccctttaa tatttacacc
    30901 tattttttat taactatacc ctaaaaaccc tagaaagcaa atccaccttc atttaactct
    30961 aagaaagagt ctgcatataa agatgccccg ttgattatgt cacaagattt tacagttttc
    31021 attaattctg ggtcacatgg attcattata gctgaactaa atccattagc cattgccatt
    31081 gctaagaatg cactatctaa aacaggtcta acatgcttag gacatccatt agatacgtta
    31141 gataatcctc ctgtagttaa aagtcccatc tcagtcatca tttttatagc ttctaaaact
    31201 tctacttgtt tttcttgcat accttttata actaagcata atgggtcaaa tagtatatca
    31261 gttggctcca ttcctaacat taagcctttt tctaatattt cttggcagta agccatacgc
    31321 tcatcattat ctcttgggat accttctttt gcacataaac ctataaccat tgcttcgtat
    31381 tcagctgcta tatctattaa atcaaatctt gagccagcat cagcagagtt tattattggt
    31441 tttgcattag ttctatcata aactttaagt ccagcttcga tagctttttt attagctgta
    31501 tctaatgcta taggaacatt gttgaactca gattgaagta atttgattcc ccatgtcatt
    31561 atttcttcgc catctctttc ggcaggtcct atattaaaat ctatatagtg tgctcctgct
    31621 tctaattgct cttttgctct ttttaagatt ggagctgggt ctctttctgc taatgctttt
    31681 cttattgaag gtgatataca atgtattctc tcacctataa tcataaattt ttccatttat
    31741 aatgtccccc tttaaattta atattaatag ttagttaata ttttattagt ttgtttggca
    31801 tgctttttct tggtatcctt ttaagaactt aggtaattcc atagattcct ctggtcctat
    31861 aactacattc catcctggta agttatcttc tatatcacct ttaagtactg caacttttcc
    31921 tggaatgata aggtctttac aattagttac ttcttctacc ttactttctt taatgaaagc
    31981 agagattgag tttccaccaa atttaccagc agcccaagat gtaagaactg aatatccacc
    32041 tgcatctggt attactaacc atactggaac ttttgatctt tctatatcac cagcaactat
    32101 gaagtaagtt aatgcaaagt caacagtaac taatactgga gaattttcat ctggattgtt
    32161 gattggataa atctttggtt caactctcat tggtctttgt gggtcagtgt atatgttttg
    32221 tcttagtgca aataatggta atgccttagc ataactaata tcatctatta ctattataga
    32281 accgtatttt atagtgaata ttgatgataa agcaacttcc atcatagggt ttgaattaga
    32341 taatctattt gcaaatacta ttgaaggata tccaaatgtt ctatcttgct cttttaaagc
    32401 tgttcttcta acttgtatag cattagtgta agtatctttt atgttttctc ctgttacatc
    32461 taatactaat tctttatatc cagcggcttg aattaattca actgtttcat ataattcttc
    32521 taagctacca gcttttacac ctaatggtaa acttgctcct ttaactactt ctatcatatc
    32581 tttgtagttc tctttagttg ctccatatac cattggtcta gcatctttta atacttcaac
    32641 agcttcttta actacttgtg catcatcaca agctaaaata taagctactt ctaatccact
    32701 gccttttatt ttatctatta attttaaata cgcatcttta tctccacaat actctaaaac
    32761 agccatttca actttcattt gttcaccaat tcttacatag tcaacttttt tcatgttagc
    32821 tattttagca tctactgctt catcactcat acaagtacag aatgaaactg catatctatt
    32881 tctacttact aatgtttttt catgtctaaa taatactgtt tctccaccta gttcatactc
    32941 agatgcacca gcaccaactt ttaaagcttt cattaaaggt gcagtagcct ctgataattt
    33001 tgctatagcg tcatcagaca tatgtgggca tttacctact tctacagctc ctgaagcaac
    33061 cttcatagag aaagccatac aagttgggaa tccacaatcc ttacagtttt tctttggtgt
    33121 taatttaaat atatctaaag cttttaatgc cattattttt tccccctaac cttaacaatt
    33181 taattaagct aatgcgttaa ccaattcttt tatagtttct attgattttg gatgacgaag
    33241 tataactgcg tttgaaccac ctactaaaac acttgctgca gttgaaactt ccattgctat
    33301 agttctttct tctggacaac cccaatctgg ctcatcttct attggagcta tagcttcttt
    33361 aacatgacct acttcaaaag ctactggtgt tatgataggc atttgtaatg tcttatcatt
    33421 ttgaccaaat gctgcaagtc taattctatc catagtagat gcaacgtact catatccata
    33481 accaactgct gaacatccaa cattcataac tatattttca cctttaacgc ctagttgagt
    33541 taacaatacg tttaattgtt tagctaggtt tatatcaaca gaagattcag ctcctacttt
    33601 atgtgcataa gccataccag ctgatgctcc tactcctttg tagttatctt ctactgctga
    33661 cataaataag cagttatgtc catccaatgt ttgagctaat ttttcaaata atttaccatc
    33721 tttttcatga tttcctgaac cagctacaac taaaggtaat tttattgctt caattactgc
    33781 cttagctaca tcagcacatt catcaactga cttatctaat ccatttgggt cagaaccatc
    33841 aaattttaaa caaataaaat ctgcttgtgt attagcttca acatatttag cccactctac
    33901 tggacaatta gctacatctt tgtataattc cttatatgaa tcagtccagc tttcaggata
    33961 aacgtctgat atttgtatac ctatttttgg agaattacct acttctccat caaaactata
    34021 aaaaggtaat acattttccc cacctaattt aattgctttt tcccctattc ctacttcaac
    34081 ttctgatatt tttccagaat atttttgagt agacatttta aatgccatat ttttttcccc
    34141 cttaagccta ttttaattct aatttagcaa aaatttcttt catagccact ttagattttg
    34201 aatcttcagg taaattaact aaaggaatac ctgtagaatc atattcatat atcaattcat
    34261 ccataggtac aacaccgatt agctctaggt tatgcttttc aatttcttct ttaacaccat
    34321 cattcataac cccatttgga actttattta caatcaaaag tattcttcca acacttaact
    34381 ttagttcctc agctaaatct cttattctag caacagcttg tatgctacgt cttgagcaat
    34441 cactaaccaa taaaagtgta tcaacatgtc tagtgacttt tctacttaga tgttccatac
    34501 cagcttcgtt gtccatgact aagtatttgt aatgaccaga tattttgttt acttgttctc
    34561 taagtatccc atttacaaaa cagtaacatc cttcaccttc tgacctaccc attactaata
    34621 agtcatatcc ttcgccttct tcgattatag aatttaatct aaattgaaga tattgtgcct
    34681 ttgtcatacc accagggaat gcattgccta acttttctct ttgatttact tcttctctta
    34741 tttctcctat tgttgcttca acttcgatac ctaacacttc atttatgtta gcattggcat
    34801 cagcatctac aactaatact ggtccctttt tgtccttaac caaataatca atcaaaagcc
    34861 ctgtgagact tgttttacca gttccacctt ttcctgcaac agctatattg tatcccattt
    34921 taagacttcc cccttaaatc tagccacaga gccttatgta ctctgtctgt ctattataat
    34981 ttaggtacag aatgaacaca ttctccatgt acatcatgaa gtgcttccat tagcccttca
    35041 gataaacttg gatgtggatg tattgcatcc cctacttgtt ctactgttaa tcctagatga
    35101 acagcaagtg acaactctgt caataaatct gtagcatgag gtcctactac tgctgcacct
    35161 attatcttat cagtttcttt gtcagctata acttttacaa atccttggaa atgtcctata
    35221 gcttgtgctt ttccaagacc tctaaaatca aattgaccta cattgtattc tacaccttca
    35281 gcttcaagtt gcttttcagt cttaccaaca ccagcaactt caggttcagt ataaacacat
    35341 cttggaattg ctctataatc tactacctta gtcttaccta aagcattttc aactgctacg
    35401 ataccttctt tagaagcaac atgtgctagg aaaggagtat ctattatatc gcctattgcg
    35461 tatatacctt ctacattagt ttcaagatgt tcattaacta ctacttttcc tctttccatt
    35521 tcaattccta tatcttctac tccagaattg tcaaggttag gtcttctacc aacacatact
    35581 aatgcatatt gtgcttctat aacctttcca ttagaaagag ttgcaacagc cttaccatca
    35641 actacttcac aagtttgtac tccaatacct gtaataacct taattttatc tttcttaaat
    35701 tgtctaagta gttgtttagc tacatctttg tcttcattta atagtatttg gtcaaccatc
    35761 tcaactatag ttacttctgt tcctaaggct ctaaagaact gccctatctc acatcctata
    35821 actccaccac ctactattaa catagactca ggtatctctt caagtccaag aacttcatca
    35881 ctagttatta caacttttcc atcatatggg aacattcttg gtacaactgg aacagaacca
    35941 tttgctagta ttatcttatc tgcttttata gtttctactg ttccatcatc tttagttact
    36001 tctatagtgt ttttatcaat taattttcca aaaccattaa ctaagtttac tcctcttttt
    36061 tcaaataaga actctatacc acttattaat tggtttacaa ctttattttt tctttccatt
    36121 atggcagtga agtttggctt aattgtacca tctatttcta taccaaaatc ctttgcttct
    36181 tttacagtat tcaatactcc tgaagaagca agaagtgctt tagttggtat acatcctgca
    36241 tttaagcaag ttcctccaac tcttcttttt tcaacaactg ttacatctgc accaagcata
    36301 gaagctttta tagctgctac atatcctcct ggtccaccac ctactactac tatcttcatc
    36361 tttaattccc ccaaacctaa tataatctac aatcttgcaa tactataaac ctgcggcatc
    36421 cagtatagca ggtatattct cttctgcccc aattgccatt atatgaacac catcacataa
    36481 atcttcttct tttaattgtt tgataagttc ccctgccatt tttattcctt cacttaccca
    36541 gttttctttt ccagctgctc ttaatctttc gatttgttca tctggaacaa atattcctgg
    36601 tacatttgca gtcataaatt tagccattcc tggtgatttt aaaggtacta ttcctgctaa
    36661 taccttgcaa tccatatccc ttgttaattc tctaaacttt ctcattgtat ttatgtcata
    36721 aagtgcttgt gtttggaaaa atttagcccc tgcttttatt ttctttttca tttttaataa
    36781 ttgaacttct attggagaat attctggagt tactgatgct cctagataaa aatctggaga
    36841 accttttaac ttattaccag ccatgtccgt acctgccatt aaagtctcag cagtttgaag
    36901 tatccccaca gaatctaaat caaacactcc cttagcttgt ggatggtctc ctacgctagt
    36961 atggtctcct gttagtgcca acagattgtt tatcccaaat acacctgctg acaacatttc
    37021 tccttgtata gctattctat ttctatctct ccctgttatt tgaataactg gctctaatcc
    37081 agcatctttt aataatttac atgtagcaag agaagttgct ctcataactg ccgattgaaa
    37141 gtctgttaca tttgctgcat gaactcttcc aactaacggc tttgcacatt ctattaaatg
    37201 tgaaaggtca gtcccttttg gaggagccat ttcagtagta actgcaaatt ttccactttc
    37261 taatgtttct cttaataagc tcatctctaa tccccctttt aaccttctaa aaaaactttt
    37321 atgaatttgc tgctgcttct ttctttttct ttgcacttaa acttcttgga ttattttgct
    37381 tagaatagtc ttttggtggt cttatttctg ctaagttatc taattgtcct atagcttcta
    37441 gtctttcata tatctttatc caagcacaat cattctctga gtttacttca cacttaccat
    37501 ttttagctcc accacaagcc ccattcataa gacctttagc acacatagtt actggacata
    37561 tcccacctgt ccaaccaagt tcacagtctc cacaagcctt acaggcttct tcatattctc
    37621 caactctttg tacttcccct ataaacaaag tattgttggc tggataaact ggcttatctt
    37681 ttaaattctt aactattgtc tgtgtaccat ctccacatgc taaagataaa actgcatcag
    37741 cttccttagt ctcttctttt agagccttta aatccttttt agactttaaa aggttacaag
    37801 cagggtcaag cattacatat cctaagattt ttttgccttc accttcaaga gtttccttca
    37861 tttttaaaac ttcttcttcc ccaccacttt tacaagttgc tgcacattga ttacaaccaa
    37921 ctaaaaccag cttgtcaaaa tcttttaagt accctagcac ttcttctaat ggtttatttt
    37981 cagaaattat catatctact tcccccctaa accttccaca tatatatatc tatttattgt
    38041 tttgtaattt gcaagcctta actacatgtt ttgcaagtat agatgttgta actgaaccaa
    38101 cccccgctgg aactggagtt atcattgata ctttgtctaa aactgcattt gtatcaacat
    38161 ctccacataa atttccttct tcatcaacat ttataccaac gtctataact accgctccat
    38221 ctttaacaaa actttcatca accatcttag ctctaccaat agctgctatt agcacgtctg
    38281 cctctgctgc tacacctgat agatttttag tttttgaatg acatattgta acagttgcgt
    38341 gttcacttaa taaaagcatt gatactggct ttccaactac cattgatctt ccaagaactg
    38401 ttaccttact tccttttaaa tcaacattat agtgttttaa tatctcaact actgctgtag
    38461 gtgtacatgg tggaaatcca cttttatccc cttccattac cttagcagag tttattggac
    38521 taaaacaatc tacgtctttt tccggtgcaa taacatattt tataacttct tcatttaagt
    38581 gttttggaag aggtctaaaa cataatatac cattaacatt tttgtcatca ttaactctct
    38641 ttaatacatc aatatattcc tcttgtgtta tatcctcagc taactctaac acttctgttg
    38701 ttatacctat gttttgacat ctctttagtg ctcctctttc ataagataaa tcatcacttc
    38761 ttgccccaac tctaactatt gttagtttag gatttatacc ttctttaatt agtaaatcga
    38821 cttcttttat taattcttca ctaatcttat cagctacagg ttttcctttt ataatttgtc
    38881 ctttagttga cattccttcc atacattcac aactccccta caagcaaact taaattaatt
    38941 tattctatat accacatatt aataacatag tatctacttt cgtcatttaa aaattatttt
    39001 ccaagagctt tttcaacttt ggcataaact tcatcacaaa tttttacacc atcttcaact
    39061 aacttatcta cttctgtttt taccttatta acatattctt tatcttgtat agaatttata
    39121 tttataacta catttaattg tccaccaagt atagcagctc ttaaacactg aactcctaca
    39181 ccaacatcac ttatagcaag tcttgaacct ttatcaacta aatcttcatg taacttaata
    39241 gcttcatagc aaactcttac tatgtttaat ggtacttcac aagcaacttt taatgccttt
    39301 tccattgttt ctgcttttat tcttttttct tcttctgttt ctttaggaag cccgtaagct
    39361 tttgataatg gtaagaaaca ttctgcatca tcatctatca tttttaataa atctttttct
    39421 aactttcctg ccttacttaa tatctctttt acactttctt catactcagc atacttcttc
    39481 ttccctattg taaggttaca taccatacta cctaaagcca tacctattgc acctactagt
    39541 gcagcagctc ccccaccacc tggtactgct tctttggaag atagtacttc tacaaaatca
    39601 acacaagttt tatctgctat tttcatgata atcctccttg aaaggatgta ggaaaatatc
    39661 tcaaattgtt cgagatattt tacgaactat tttaagatat ctcctactct ttatttataa
    39721 attaatttcc ttagaataat ccagctatta ctccattttc atcaacatct attttttctg
    39781 ctgctggaac ttttggtaat cctggcatct tcattatttc tccagtaagt gcaactataa
    39841 atccagctcc tgctgaaatt gttgcttgtc ttacagttat tctaaatcct gttggtctac
    39901 ctaatttagt ttggtcatca gttaaagaat attgagtctt agccatacat actggaactt
    39961 tagtaaatcc aagtttttct aagttagcta tttccttatc agcttcagga gtataatcta
    40021 caccatctgc tccataaatc ttagtagcta tagcatttat cttgtctttt atagataaat
    40081 catcttcata gcagaatcta aagttatttt cttcttcatc caataatctt aaaacttctt
    40141 tagctactgc aattccacct tctccacctt ttgcccatac ttcagaaaga gcaacattaa
    40201 cacctaattc cttacacttg tcttcaacaa gtttaagttc agcttcagta tcaagtggga
    40261 atctgttgat tgcaacaact gctggtaatc catatacttg agttatgttt tcaacatgtt
    40321 ttaataagtt tggaagtcct ttttcaagag cttctaggtt ttcgttattt aagtctgctt
    40381 tagcaactcc accattgtat tttaatgctc ttacagtagc aactattata acagcatctg
    40441 gttttaagtt tgccattcta cattttatat ctaagaattt ttctgctcca aggtcagcac
    40501 cgaaaccagc ctcagttatt acataatctg caaagtgcat agccattctt gtagctataa
    40561 cagagttaca tccatgagct atatttgcga atggtccacc gtgtacaaat gatggtgttc
    40621 cttcaagagt ttgaactagg tttggtttta aagcatcttt taataatgca gccatagcac
    40681 cattagcttt taattgtcta gctgttacag gttcaccttc gtagctgtat ccaactatta
    40741 tgtttcctaa tctttctttt aaatcagata tatcacttgc caaacagaac gctgccatta
    40801 tttcagaagc aacagttata tcgaatccat cttgtctaac tgctccatca ccttttttac
    40861 ccataccatc aacgatattt ctaagttgtc tatcgttcat gtctacacat cttctccaag
    40921 ttattttctt agggtctatt ctaagtgcat ttccttggtg aatatggtta tcaagcatag
    40981 cagctaaaag attgttagca gctcctatag cgtgaaaatc tcctgtgaag tgtaagttta
    41041 tatcttccat aggaacaact tgtgcatatc ctccaccagc tgctccacct ttaacaccaa
    41101 atactggtcc tagagatggt tctcttaaag caactaatac atttttgtct agtttagcaa
    41161 atgcatcagc aacacctata gtagtagtag tttttccttc acctgctgga gttgggttta
    41221 tagctgtagt tagtattaac ttagcttttt taccagtctc tctttttagt agattgtagt
    41281 caacttttgc tttgtattta ccataaagtt ctacatcatc ttcacctagt cctaattttt
    41341 ttgcaacctc tctaatatct tgaggcttag cttcttgagc tatttcaatg tcagatttaa
    41401 atcccataac tgaatctcct cccacaacct aaatagattt aaaatataat ttttgtaaaa
    41461 attgtattaa tatgtaaaaa ttatatttta taagataatt atacatgtaa aattacttaa
    41521 aaactagcta ctaagctatt aaaatattca aacttaatag ctagttattt atctaattac
    41581 ttacttatta cctatctatt gcctatgtct aacctttaac caacttaaaa tcccaaatca
    41641 aaatttctat ctattttatt tatccttaag tgacattaat ttatctttta ttgctttaat
    41701 ctgttctttc gttgtttcac tagaatcata tggagattga ttagctctat cagaatcaat
    41761 aacatcttgg ttataataaa taaatcctaa agattctcca tcttctaagt tttggattat
    41821 aaattcttca tcttccttat ttctaatttt atttcctact acaaatactt tatttacacc
    41881 tatatcatgt ccaagtttct taacttttct ataagtctgt agacttctct ctcctggttc
    41941 tacaactact ataaaagcat ctacaccttg ggctgttcct cttcctaagt gttcaattcc
    42001 cgcttccata tccataacca ctacatcttt attttgtagt attaaatgtg agcagagtct
    42061 cttcaaaaga acatgctctg gacatacaca tccagtacca ccagaatcta cggttcccaa
    42121 tgtaagtaat cttactccat tgtattcttt acagaaattt tcagggatat cgtctacttt
    42181 aggattcatc ttaaacattt ttccaaatga acctacagaa gcagctgttc tatctgaaac
    42241 taattttttc atttctgata taggtactat tgattcatat acttcttttg gaaaacctag
    42301 tgctaaagct aaattagcat cagggtcggc atcaacagca acaactctgt atccgtcttc
    42361 agcaaacatc ctagagagca ttgaagaaaa agtcgtttta cctactccac ctttacctgt
    42421 tattgctatc ttcataatcc ctaccacctt ttattactat ttgcttttta gtagttaatt
    42481 ttacagttaa atatttttta tactctatat ttttatacac taaaaataaa ctacagctag
    42541 tccttacagg aaccaattaa atttacccta ctagcttttc aaagcagcat gtatagtttt
    42601 attattcttt aatattgttt atattttatt tattcagtat attcattata ttttgatgtt
    42661 ctttacttta atatttgact ttattcaact ttattcaacc agaaacttct tataaaaacg
    42721 tttattctat aacttcacta ttttttaagc ttcagccttt tctgccatct tttcttcaac
    42781 taatgcttca aagtgaacac gttttttctc tatatcttct attattgtag ctgctaactc
    42841 ttttggattt gtatttacag taaatgtagc tccaacttta tcttttaatt cactagttaa
    42901 tatatccact gctcttgaag aaccagctat tggaggcatt attcctaaat aagtgtctat
    42961 accagaagct acaacatagc atcctatagc aactgctttt tctgacatcc actctggagc
    43021 tatacctaca actggaaggt cacacatatc catatctaag taattagctg ctgctccaac
    43081 taaatctagt atacgactta tatcaacaca agaacccata tgtagtactg gtgggattcc
    43141 tactaattta cagacagttg caagaccttt acctgcatat ttttcagccg catcagattc
    43201 cattaaacca aacttagctg ctgctgaagc tccacaacct gtagtaacta ctattatgtc
    43261 gtttttgatt aattctttca ttattgttac gtgagcctca tttgaaactc ctttagcatt
    43321 gttacatcca actacaccag ctgctcctct taatactcca gattttaaac agtctgttaa
    43381 tggcttaact gttcctgctg ggtctatatg agagtttaca actctgtcta attgatttac
    43441 tactgcttct acactatacc caactgttgc gcctgatttt aattcaggta taagtacttt
    43501 tgatttatct ctatttttaa agtttaaaat agcttcttta actattgctt tagcatcatc
    43561 aagtgcttgt tcttctctaa attcaatata agttgaacct gttatcttag cttttggaga
    43621 agttgttaca aatttagtat ggtaacaatc tgcaactcta gctaatgcag ggaatataca
    43681 ttgaacgtca actattacag cttcaactgc tccagttatt attgcaagtt cttgttgatg
    43741 gaagtctcct gctattttta caccatgtct cattgtaatc tcattacctg tacaacacat
    43801 acctgctaag tttattccat cagcaccaac ttctttagct aaagctacta agtctggctc
    43861 ttctgatgct aaaactatca tttctgataa tgctggctca tgtccatgta atattatgtt
    43921 aactttattt tcttctaata ctgctaaatt cgcctctgtt cttcttggca ctggagttcc
    43981 aaataagata tcgcttagac gagtacctat catagaacca ccccaaccat cagccataga
    44041 agttctaaga ctcatgtgta ttaaactatc tatatcacca gtacatccta tatgagtaga
    44101 gtgcattatt gttgcaattt ctctatctat agctcttggc tctatagcgt attctttcca
    44161 tattttttgt cttggttctg gtgcattttt tataaatctt aatgtcccaa aaggttttcc
    44221 aaattccatt aatgcaattt ctgccacttc atgtgctaca tcatagatat ctctaccttc
    44281 tgtctctaca tcccattctt tagctaaagt aattaactta gcttcgtctc ttattttata
    44341 gtttccattt ggatcagcta atgctaatgt atgtgctata tctctagcat ggtctgaatg
    44401 ggcagctgtc ccaccagcaa ccattcttgc aaagtttcta cctactatag tatgctcatc
    44461 tgctccacat aacccccttg gagtcttagg gcttattcta catggtccca ttccacatat
    44521 tctacagcaa acaccttgaa gaccaaaacc acattgtgcc ttaaagcctt cttttctatc
    44581 atacattgtt tctacgcctt cttctctagc tttagctatc atcttctggc tgtttaaatc
    44641 aattgttaac attttttcat ccatattcct atcccctttt tttgattttt aaaatccccc
    44701 aggtgactga cttcatgtca taaaataata aaataaatgc tttctaagtt gattatatac
    44761 taattttcag tgctttgctt tagatttaaa tattttttta acaaaattag ataatacata
    44821 aaaattttat tttctacact taatttaagt tgattttatc gcttgttttt ggctatttgt
    44881 taatatctaa acctactttt ttgatacttc agtttaatta aattatgttt tgattttttt
    44941 aaatcaaatt tgaaattttt ctagtcattt ttatattatt gcagattgtt tgtgtattta
    45001 ttatctttca ctactgtttg gacttattac agatttttac tccctaaact taattagtaa
    45061 aaacttctta gaatagcatt ttttggacta atttttttga atttttttat ttttcctact
    45121 ttaatattaa ttatagcttc aaattgaaca tttgtatata ttcatttgaa gttataatta
    45181 ttttttatat aaaacttttt taatacagca ttaatttaga cattttgact attttttcta
    45241 ctttaattca tttatttcaa gcgatttatc ctatttcata tttttagcta caatagcccc
    45301 atatgcaata gaatataatt tagattcatg tgaatcagct cttgacacta aaactatagg
    45361 agcttttgca cccataatta tacctgctga tttagagtct gccatatatg taaaggcttt
    45421 ccctattcca ttaccaacct ctattgttgg aaccaatatg atatctacat ctccagatac
    45481 ttcactttta aaccctttta tctcactagc ttcttttgaa acagctaaat caaaagccag
    45541 aggaccttct acaattacat tttcaccaaa ttctccttct ttacaagctt ttgctaacat
    45601 atctgcatct acagttgctt gcatcttagg atttactttc tctttagctg ccaaacatgc
    45661 aacttttaca gtttccattc ctaaagcttt tgcagctttt atagaatttt ttaatatctt
    45721 tgccttttgt tcataatcag gagctatatt catacctcca tctgtagtta taagtaattt
    45781 atggtatttt tctaactcat ataccataac atgacttaat aaactatcag ttctaagacc
    45841 atagtcttta tttaacactt cttttaatat gacagatgta tctaataatc ctttcattac
    45901 aaagtcggct tctttacttg atactaattt aactgctatt tctgcacact ctttgatatc
    45961 atccttatcc actattttga taccacttaa atcaaatcct atttcttttg atatttctct
    46021 tattttgcct tcatttccaa ccaatatagg agtaattata tttctttcta cagcatcctt
    46081 tattgccatt aaaacttctt tatcatgagc tgctgctaca gagagtacaa ttttctctcc
    46141 tcccttaagt tgttctaaaa tatcatctaa tttttttatc aaaagtaacc cctcccttat
    46201 atatatttaa ctaaataata ccatatttgc tatacctttt ctcaatttat tttttatgtt
    46261 ataaagaatt tatataacta aaagacagtt taatatttta ttctgggatt tcatcaataa
    46321 ctcttaaaag ctttgcagga acaccaccta caatagtatt ttctgcaaca ttttttgtta
    46381 caactgctcc agcagcgata attgcattat ctccaatagt aactcctgga actacagtta
    46441 cattagcacc aatccataca ttttttccta ttgtgattgg agaaggataa gttgtaccac
    46501 gtttctctgg tgcaatacca tggttaagtg tagccaatac cacattatgc ccaatttgcg
    46561 tattatctcc aatctcaatc cccccctggt cttgaaaatg gcaactgcta tttataaaaa
    46621 cattttttcc tattgtgatg ttttttccac aatctgtata aaatggtgga aacataaaaa
    46681 atgattcatc tacctttttt ccaattaact cagaaaataa actacggact tcttctggtt
    46741 catgataaga agaatttaac ttacttgtga ttttcattgc ttcattactc aacttaacca
    46801 taaattcatg tatttctgat ttactagaaa caggttttct ttgattgata tgtgttaaaa
    46861 attcattgat attcatttta tacatccttt caatatgtgc tttaatttat ttgtacattc
    46921 taaataatat tttaataaaa attaatttaa tttattttta ttaaaatatc attgcttctt
    46981 ttagtgtaat atacaattat atctatatca aatatctata gtaaatactt aactatactt
    47041 aaaaagcata taaattgata taattatata atagataagt taatattgag gagggataat
    47101 tatggaaata agagtattaa gatatttttt agcaattgca agagaagaaa gtatatctgg
    47161 tgcggcaacc tttttacata taacacaacc aacactttct agacaaatta tggaacttga
    47221 agaagagttg ggagttaaat tattggttcg aggaaataga aaaatcacct taacaaatga
    47281 aggtaggctt ttacataaaa gagcagaaga aattatatca ttagttgata aggttgaatt
    47341 agaatttgac aatcccaatg agattattag tggtgatata tatataggaa gtggtgaaac
    47401 tgaagctatg cgacttatag ctagaactgc ccatcatttt catcaaaaat accccaatgt
    47461 aagatatcac ctttttagtg ggaatgctga tgatgtgact gaaaaattag acaagggact
    47521 gcttgatttt ggtattttaa ttgaacctgc aaatatacag aagtatgatt atgcacaaat
    47581 tcctgctact gatgtatggg gtttactaat gcgtaaagat agtgcccttg catcacaaag
    47641 cacaataaat ccaaaagatt tatgcaatat acctcttatt atttcaagac agacacttgt
    47701 tgataaagca atttctaaat ggcttcaaga cagtaatgaa aaactaaaca ttattgcaac
    47761 atacaattta gtttataacg cttctctttt ggtagaagaa ggtctgggct acgctctgtg
    47821 tttagataga ctcgtcaata ctacagaaaa cagtaatctt tgttttagac ctctatctcc
    47881 tcctttagaa gctaaattaa atattatatg gaaaaaacat caaatatttt caaaggcagc
    47941 agaaaaattt ttacttcaaa tacaaaaaga tttttcttct atcatgttgg atacatctaa
    48001 gttggataca cctaaagaaa aataaatcat aataaaaggt gagagtgtct caaaatgaac
    48061 tttgcactgt ataaggcact cttttttata caaagtaaaa tatatgttca tcatttcagc
    48121 actaaaaaag aggcttatct ctattttgag atagccccac ttaaccaata aaattaaata
    48181 caacttaaca atatccttac ttatttattc taagttctct tagacaagca atagtaattt
    48241 catcttgtgg atttaattca taagctcttt ctatatagta agttgcatca tcaatatcac
    48301 cattatttaa ataagccatt cctaaattgc aaagtatttc tgggtcttcc tttatcttag
    48361 ccgcttgttc aaaatactct attgctttat ctaaattatt cttataagcc tcacaaagcc
    48421 ccaattccac tatagtatcc acttgattag gctttattat caatatcttc tcaagataca
    48481 tcttagcctg ctctatttcg cccataccct tatatccaag tgctatcata aacagcaagt
    48541 tccaccaatc catatgttct tcttctaatg gaagtaactt ctctaatcct tccttaaact
    48601 taccttggaa tacaagtgta tatccttctt catattgaac tttaaagtcc atcttgccca
    48661 gattctcttg tacctcagct actaaatcag catctaaccc tagccttaac gcttcttccc
    48721 aagtcagctt agtctttaaa tattgacctt gattataata atggtagcct aagtgataat
    48781 aacctaatgc aaaattctca tcaacatcaa ttaccttttc aagcttagcc atggcttcaa
    48841 gtaagaatgc attcattgcc ttatcatcca tatctttttg atactgttta gctatctctt
    48901 ggcaaacaat cgcatagtta tacattgcat ctaaatcatc tggatacatt gttataaatg
    48961 cttttatata tattaaagaa tctgtatgtt caccaatctc aaagtatttt ctcgacatat
    49021 atcccatata cgatttcaaa tccaaatcaa tatcttttga taaagcattt aaaaacttct
    49081 catactctgc attgttttta aacttactat ctattccaat tatatatatc attgcatctg
    49141 ctatagacat tgaatttaac ttatcttgag cagtcttttc ttttatccct tttactaata
    49201 cctcattttt tataggtaca tctaatccac cttttggcac ttcatagctt tttaatttaa
    49261 actctccacc atgttttata gttataaaag ctaattcttc tgcttttttt agcaaatatt
    49321 tttctatctt aaaatccatt ttatcctcct aaatacttaa atctctactt tatgagtttt
    49381 agatttctca aatatttttt tatcatcata ctcaaataag actacatttt ctacttctct
    49441 tatttctgta tttatatcat tttttaaatc cacaacatta aatctaaatg gctcaaaatg
    49501 aacatatttt attatatgtt tagctggttt atcaacaaaa ctaggtaaat atgttgataa
    49561 gttatcatta tcttgtaaaa atacatggca tctattctta aaatcatcca catctagctt
    49621 attaaaaaac tgtggtatat ttgatgtttt acccagtgat atatagtcat attttaatat
    49681 atcgttgaat acatcattat ctatgttgat tttttcatta tagaaatcca tcaatatttt
    49741 atataactga ttctttcctt gtgccaaatc aaagtagcca ttttcatcaa aatacttggc
    49801 aaaaacctca aagaatttaa aagggctttt tttataaaat ctgcctataa tatatctcat
    49861 agataacaca aagtttttag aattgtagta tctctccagt atatcttcta tatcctttag
    49921 cttcaaagtt tcttcatagc ttatataatt attgtataaa aactcatatg gagcataatc
    49981 cttatattta aaaccatgct catctgcaac ttttctcata cctgtccctt taatcatctt
    50041 taaaaagcct aattgcaaat gctctatacc tagattaaat acatcattaa aagaattttc
    50101 aaagctctta taatcttcat aaggcagtcc tgcaattaag tctaagtgct gatgtatatt
    50161 tctataagat gctacagttt gtacaacatg agacaacttt ttaaaatcat cccttctacc
    50221 tactgcatca agtgtttttt cattcgttga ctgaacacct atttcaaatt gaaaaagacc
    50281 ttctttacaa tcagccaaaa actcaagcat cttatcatct atcaaatacg ctgtaacttc
    50341 aaaatggtaa gttgtatatc cattatcatt tttcataagg aaatccatga tttccatagc
    50401 cacttttcta tttgcattga atgtcctatc tataaacttt atctgtgata ctcttgcatc
    50461 tattaaagct tttaaatcac tctttatcct gtctaaacta aaatacctta gacctggaat
    50521 agtagatgat aaacagtatt ggcagttaaa agggcaacct cttgatgatt cataatacac
    50581 tattctattt tcatattctt ttggattcaa gttttcataa ggactaggaa tctcatccaa
    50641 atcttgaagt aattccatag gcttattcac tattatctca ttgccttgtc tatatacaag
    50701 tccatttaca tcatttattt ccatctttcc ttgcaagtga agtactaagt ctctaaaaat
    50761 taattctccc tctccataaa gtatataatc cacaaaatca tactttttca ttgcctcata
    50821 actatcataa ctcacttccg gtccgcccaa agctatttta gtattaggtc taattttttt
    50881 tagattatca caaagtttaa ctatatctcc cacattccag atatatgttg agaataatat
    50941 tatatcatat tcatttttat atatatcctt taaaatatag tccaaatcgt tatttatcgt
    51001 gtattctttc atattaactt ctatcaaatc tcttacaaat tcttttagat atcttatagc
    51061 caaatttgta tgaataaact tggaatttaa tgttgttaat aaaattttca aatatttctc
    51121 acctcttttt aagattatac tgtctttttg taccccttat aggttaaaat agtatatgta
    51181 aagtctaaat aatatctata taattaattt attaatttca agtaatgtga ggaaaggaag
    51241 ttttttatgg aattaattga aaaagtatct atcaactctt tatcaaaaag agatttacta
    51301 cttattataa agtctcttga atacactaat gaaaatactc aaattagtga tttcattgac
    51361 cttaggaata atatagtaaa ggaattatgt tttttaactg acactaagga aaaagatttt
    51421 ttagattatc tagaaaaaaa tagcacactc taataaccta aaccttttta aactttaata
    51481 tataaatacc tgatttagaa tttattttta tgcttaatat attatcatta aaggttttcg
    51541 acataactgt tatttgatta aatatagggt ctattatagt attaccttct tcaagtctta
    51601 taagacttct tgggtctatg cttttattat atgccttaac tccaatttta tattttctca
    51661 atttaacaac atattcggac atagaaaaaa taaaatctat attatcttca tttaatttac
    51721 tatcttcacc caaaatggat aatatagatt tttttatgct attaattcct tcattttcta
    51781 cagttctatc ttccatatat acatcatata gaacatttaa aacatttaat aaaatacttt
    51841 cttcatcata atcttcagat ttcaaatcaa tgtacttatc aattaattgt attgctttaa
    51901 tccttgccat ctgaaacttt acaacatctg atttgtctaa ttctatagaa aatcctatta
    51961 tcttgtcttt aatactctgt agtaattcga catactctat tttactatta tttataagat
    52021 tgtgcataat ggaattatac accacagaac ctaataaata atcaaataaa agttcttctt
    52081 ttttaaaata aataacatac ttttttatta gtttaataac ctcatcttca cttttttgaa
    52141 tattagtatt tgataaaatt agtggtaata aaatatttaa aaaattgata tttcctattt
    52201 tttctccata aaatctatag taaataagtt catctctcag tctattttcg tctatataca
    52261 cacatctttg cgttttatca tctattaagg attttgtatt tattatagtt aaatctttaa
    52321 gagccctact tgcactacta aaagaagatg tgtagacttt ttttagtaat tcgtcattca
    52381 taaatatatc ctttctgaaa aattgaatgt aaataaattg ttattaaaaa aaaagagaca
    52441 gcccgtttaa gaagctgtca tatatagggg gatagtagtg tacaaaaatc attgtaatct
    52501 actatctata tattattata cgatactact tttttatatg taaagcatca gttttcgaca
    52561 tccctatatt ttacagttat acaaattgcg tactaagttc aattttattc tttttatttc
    52621 aacattttat catgctacat tctttcagga gcgtgcattc ccaacaattt cagagaattt
    52681 tctaaagttt gttttgttgc taaaactaat gcaagtcttg cttttcttat ttccaaattt
    52741 tctactaaaa ttggattgtc atgataaaat ttattaaacg cttgagctaa atctagcata
    52801 aatctagtca ctatatgtgg ctcatttttc tttagagcaa gtaaaatatt cttattgaat
    52861 gattctataa ctctaagaac ttctgcacta tcttcatcag ctaatagact ataatctata
    52921 tcagaagtca cttctacttc tgcttttctt aaaactgcac agcatctagc atgtgtatac
    52981 tgaacatatg gtccagtctc accatcaaaa ctaagcgttc tttcccaaga gaaggtatag
    53041 tctttaattc tactattaga taactcttgg aatactactg ccccaacacc tacttgctta
    53101 gatatttcat ccacattttt agcatttggg tttttagcta gtataatttc cttagtttta
    53161 tctatagctt gatttaaagc atcttctaaa aatactactc ttccttttct agtcgacata
    53221 gtaccttctt caagtgcaac cataccaaat ccaacatgaa ttaaatcttt tgcccaatca
    53281 tatcccataa gctcaattac cttaaaccat tgttggaaat gtaagttttg ttgagaacca
    53341 actacatata tacacttgtc aaaatcataa gtttcttttc tatatattgc agcagctaaa
    53401 tctcttgtca tatataacgt tgagccatca ttcttagtaa taagtgctgg tggcatttta
    53461 tactcttcta aatcaacaat tcttgctcct ttagactctt ttaaaagatt tttctcttct
    53521 aatagttcga taactctgtt cattttatca gaataaaaac tctctcctgc taaagaatca
    53581 aattctatat ctaaaagttt atacactctg ttaaactctt ttaaactctc atttctaaac
    53641 cattgccata attcttgtgc ttcttcgtca ccattttcta atttattaaa ccatgctctt
    53701 gcttcatctt ccatttctgg gtgttgttca gcttcatcat ggaatcttac gtataatttt
    53761 agtaattctg gaattggatt agactctact acttcttttt ctccccactt tttaaaggcc
    53821 actattaact taccaaactg agttccatag tcacctaaat gatttattct tatagtcttg
    53881 tatccttgcg aatcatatat cttatataaa gcattaccta taactgtagt tcttatgtgc
    53941 cctatatgga aaggttttgc tatatttgga gaagaatatt caactattac agttttatct
    54001 tttccaaaat cactatgtcc gtaattttct ttttcatcta aaactttttt aattacagtt
    54061 tctgctaatt gagatttatt tacaaagaaa tttacatatc cgcctaattg tattactttt
    54121 gttatctctc cacttggttc tatactttcc gccaattcac ttgctatcat gtttggagct
    54181 tttctaaaca ctttggctaa tttaaagcat ggaaaagcat agtcccccat atccttattt
    54241 ggtggaactt ctataagtgc ctctatctct tctttcgata aatcttctat tttttcttta
    54301 aggcaatttg atattgccac cttaaaatct tgcattctgt tgctcctctc tacattgttc
    54361 ctggtttata ttgtgcttat ctctttatgt taaatcaaat ttcgtgtttt ttcaagtatt
    54421 tcattacagt gtaaaacact ttagtaatta tgttttctgt atatattaaa aatactataa
    54481 ttgcagtttt taatcacact attattgatt ttttataaga cttttttcta ttttattgtt
    54541 tcttgtacta tccagttgac tttatatact ttttttaatc ttttatctat tttcatacgt
    54601 caatgctaat tttatatact tttcttttaa tcttttatta atttttatac atcaatgtta
    54661 actttatata agttttttta atcttttatc tgttttcata catcaatgtc agctttatat
    54721 acttttttta cttttttatc tatttttata catcaatatt agctttatat acttttttta
    54781 atcttttatc tattttctat aaatcttttt cactcttaac ttttattcct tcttcttcta
    54841 atagccttgc tgttatacct ttccctggta tctttttacc tgaaaaacta ccatcatata
    54901 ttagattaca tccacaagag gggctaccct cttttagtat tgcttcgcta catacatata
    54961 aatttgctat ttttagagtt tcattagccc ctcttacaaa gttttctgtt acatccttat
    55021 tatctttagt aattactttt ccatcaacta tctcagcagg tggtcttggt gtagatagac
    55081 cacctaactg ctctgggcat acaagtgtaa attctttgcc ttttaaatat tccttgactt
    55141 tttcattatc attattatcg ccactatatt tacaatttat tcctattaaa catgcagata
    55201 ccaatatcat aaaaatgtgc tcctatacaa attcttaata tattctcata caaattacta
    55261 atgtattctt atacaaatta ttttaattta acaattgtta ctcctgctcc accttctcca
    55321 tattgaccac ctctttgtga ttttacatgt ctatttcttc ttaaaatgtc ttgtagccct
    55381 gcttttaata ctcctgtacc tattccatgt attactgtta cagactctaa acctgccaca
    55441 tatgcatcat caagatactt ttctacttcc attatagctt cttctaggtt taaccctcta
    55501 aggtcaactt cattttttac actaccagat tttgacctta taatgtttct cgttgattta
    55561 gttacatttg ttgaaacatc tttgcgtgtt ttttgtaaag acttaaaagg tagagtcatc
    55621 ttcattatac caatttgcac tactgcttct tttctctttt tatctactga aactacacta
    55681 ccattttgat ttaaagttat taccttaact tcttcacctg gctttaagtc ttttatctcc
    55741 ttatttgata cctttggaac tatcatactt tttactgttg gctgaagact tcccatagag
    55801 ctggtcaatt cttttctaag ttcttctatc ttacgattct tttcttttga agctctttct
    55861 tgttcaagac ttctaagttc ttttattata atatctactt cttctttagc ttgtcttata
    55921 attgaaaatg cttctgattt agctttttca atcatcttat ctctttgact gaccaatttt
    55981 tcaagctttt cgtcatactc tactttcagt ttttcaatct cttcttttaa tctttcagct
    56041 tcttctctat cttctactgc tttgattcta ttcttttcaa cattctgtaa cacatcttct
    56101 aaagctatgt tttctgtatt tatatattct ttagcacgtg aaatcacata atcacttagc
    56161 ccaagttttc tagaaatctc aaacgcattt gattttccgg gaacacctat aagtaactta
    56221 tatgttgggc ttaaagtttc tatatcaaat tcaactgctg cattttctac acctggttta
    56281 gtaagagcat aatttttaag ctcactatag tgagtggtag ctatacattt agctcctaca
    56341 gaatttatat cttctaatac tgctattgca agggcagctc cttcaacagg gtctgttccc
    56401 gctcctaact cgtcaaatat aactaaagag tctgctgtta cgttttgtaa tatagatact
    56461 atattcgtca tatgagatga aaatgttgat agactctgtt caatactctg ctcatctcct
    56521 atatctgcaa atacattatc atatacacac atactacttc cataatcagc aggtatatga
    56581 agtccacttt gtgtcataag tgcaaataga cctacagttt ttattgtaac agtcttacca
    56641 cctgtgtttg gacctgttat caccaatgta tggaagtctc ttcctaaata aattgtattt
    56701 gctactacct tctttttatc cagcagtgga tgtcttccat tttttatgtt taaatattta
    56761 tcctcattta aagttggttc tattcctctc atctgtatag aaagttttcc ctttgaaaat
    56821 gcaaagtcca atcttccaag tatctcttga ttagaaatta agtcttcact aacttctcca
    56881 accatagcag aaagctctga taaaattctt tctatttctt cttgctcacc tagttttaac
    56941 tgtctcaatt cattgttcat ctctactata gtcattggct ctataaaaag tgtcgcacca
    57001 gaagatgact ggtcatgcac tataccagct acttgcgacc tatattcaga ttttacaggt
    57061 actacaaatc tatcacctct tagagaaatt attgcatctt gtaagtactt ttgataagta
    57121 gttgaactta ttattgaatt taattttgac ctaatggatt gatttttttg ggctattctt
    57181 cttctgatat ctctaagtgt acttgaagca ttgtcagata tttctacttc actaattata
    57241 gcattgtata tctggtcttc tatgtccttg tatacatata gagaatttga tagagattgt
    57301 attattggat aattaaaatc ttcttcatca ctactagata agctatttct taatcttcta
    57361 gccactctta aggtatctgc tatcataatc agacttcctg ggtcaagaga agcacctata
    57421 taagctctct ttaccttatc ttctatatca tgaatacctt ccaaccctac tgaacctctc
    57481 tttataataa ttgattgagc ttcactagtt tcttctagca tagatttcac ttctacaaaa
    57541 tcagtatttg gtactaaatt ttctatatat tttagaccta aagaagatga ggctttcttc
    57601 tttaacaaat ctattatttt attgtattct agaactctta acgatttctc attcattttc
    57661 tatattcacc atcctcgata ttttaacttt tttatttaat tttaaaaatg tatgttttca
    57721 agcatttcca ataaaaactg caagttttat ttctttttca tttctaaaat tattatattt
    57781 tttctctttt agatatattt tacacatatt ttgtattata ctatgtatgt agctatctat
    57841 atattataca taaacatgct ttatttgtaa tataatctgt taaaaaatct tctttaatat
    57901 ttttaaagga gtttatttta gcttataaaa aaaaatagct acttatatag ataaattaag
    57961 gaggaacatg agtcatggga aacgtattag aaggattaaa accagaaagt gtttttaaaa
    58021 actttgagaa aatatctcaa attcctagag gttctggaaa tgaaaaaggc ataagtgatt
    58081 ttttacttag ctttggtaaa aatttaggtc ttgaaactat tcaggatgaa agcttaaaca
    58141 taataataag aaagcctgct acaaaaggat atgaaaattg tccaggtgta gttcttcaag
    58201 gacatatgga tatggtttgc gaaaaagaaa agaatgttga acatgatttt cttaaagacc
    58261 ctataaaatt aagaattgat ggtgacatga tatatgctac tggaactact ctaggagctg
    58321 acaatggtat agctgttgct atgggtatgg ctatattaga agataatact ttagagcatc
    58381 ctgcactaga agttttagtt actgttaatg aagaagatgg tatgaatggt gcagatgcac
    58441 tagaccctag cttaattaaa ggtcaatata tcctaaatat ggactccgag gaggaaggat
    58501 acttattagt tagctgtgct ggtggtaaaa cttgtgttgt tagtttacct gttgaataca
    58561 aggaagttaa aggtgacaaa caaggattat tagttgaggt tactggacta ttaggtggac
    58621 actctggtat ggaaattgtt ttacaaagag caaatgctaa caaagcaata gctagagtat
    58681 taagtgtatt gaatgttgat tatgaattag cttctgttga tggtggtact aagcacaatg
    58741 ctatacctcg tgaagctaaa tgtgtaatag ctgttaataa agctgatgtt gaatctgcta
    58801 aaaaacaaat aaatgacatt ttaactgcat ttaaacatga gtttactact tctgaccctg
    58861 gcatgactta ctcagtagct gaaacttctg tagacaaagt tcttactaaa gattgtaaag
    58921 aaaaagttgt tcaaatgtct tgcttaactc ctcatggagt tcaatcagta agtcttgata
    58981 tagaaggatt agttgaaagt tctactaact tcgctataat cgaaactaaa gaatctacta
    59041 ttgaattttt aacttctgtt agaagttctg ttatgtctat tagagatgaa atcgctgata
    59101 gaataagact tttggctcaa gctttaggtg ctaattacga ccttatagct caatacccag
    59161 cttgggaatt taaaaaaggc tcaaaattag aaaaaatatg cagtgaaact tatgagaaat
    59221 taactggtaa agttcctact gttatggctc tacatgctgg tcttgagtgt ggattattac
    59281 ttgataaact tcctcatgct gaagctatct ctataggacc agatatgttt gatgttcata
    59341 ctccaaatga acatgttagt atcccttctg tagctaatgt ttgggattat gttattgaaa
    59401 tattaaaatc tatgaaccaa tattaaattt aagttttgat tctaagtaga ttttaatttt
    59461 aagtaatcta tataaaatta actctgtaaa ttaatactac tgttagtata atatttactt
    59521 tcaatattaa cttatagaaa taaagcctaa tgatagataa taacatgtat cattaggctt
    59581 tattgtaatt taaatattat taagctttat tctgttttaa aattaattct tatactattt
    59641 taaatctatt tcttccttgt ctcttagaat cataaagagc attatctgct atttgataca
    59701 gttcatcata ctcttgacca ggctttgcta taactacccc catactaata gataaacttt
    59761 ctccatttgg caacttaatg agatgaatat gattgtatac atcattacat ttattaataa
    59821 gttcttcctt atttattata tcattgatat aaacactaaa ttcatctcca cccaaacgac
    59881 ctactatatc attttttgca aaactttcat caagtacctt tgcaacatca ataagtactt
    59941 tatctcctaa caagtgacca taagtatcat taatcgactt aaaataatca atatctataa
    60001 tcaataaagc accatttttt tcagttgata aattcaacaa attttgtgca gttaattttc
    60061 taatatatgc tgcattataa atattggtta aactatccct ttgagcctta tctaacaatc
    60121 tattcttttc ctctttttca gaatcaatat tacatatttt cccgattaca tacattggta
    60181 tttttctatc attataaatt atttttgaag taactctaat ccaatgaaaa gtatcatcaa
    60241 gcataataga atacatttca gttgttccat tttcttgtga ttttattaca tcaaaaaact
    60301 ttttattata ttctttcctt attttatctc tttcattttc tatattgcca ttttcacttt
    60361 ccttatcatt tttatccaaa ccataataag tctcaatacc cttttctggc atagaaatta
    60421 tcaatttatc ttgcttataa tcatactcaa aaaagtattc acttgtcatt tcatacaact
    60481 gagtatgttt tttgagttct aaagatatct tctcgttcat acttgtacga gtatgcaaac
    60541 caaatgccat aaatccaata atacaaatta ataaactggc tacaataaaa atagtctcaa
    60601 ttggattctg ttgcataata taaagcaacg agaagtcttg tctatatgta gtattttggt
    60661 agacaattga ttgcattttt tctgatgaaa tacttataat agctttatta aatatattca
    60721 ataattcttg tttatctttt ttaattatac caaaacaaat actctttgct ttataagttt
    60781 gaggaactag acgcacattt ttatacttag gttgattcat atagtactgc atagaataac
    60841 cttcgccata agtgtaatct gcatagcctt tattaacagc ttctatacac tcttctaaat
    60901 tatcatacca aactatattc tcttcaaact gaccataata attaatacct ttggctagag
    60961 ctacacgttt tccatctaaa ctattctcat ttaatttatt attaatcatc atagtatatt
    61021 gagtagaaag aaaaggccga gacataacta tattatgttc acgagagtat tcataatcat
    61081 aaatcattcc tgctacaata tcaatttcac gctttttagt catgttttct aaatcttctt
    61141 gagattttac aggcacaagt tcaaattcta agcctgtttt atcacatata tgatttaaca
    61201 aatcaattga tatacctctt aattcacctg tatctttatc tatattttga aatggtggtt
    61261 tgtttaaaag tactcctact tttaattttt ctgtctcttt aatgtaatca gtttcttcat
    61321 cagataaaaa catttgattg ttctctggtg taaagtattt ttcatataaa gtagttgaaa
    61381 aatatgggtc tttctgttca atattattaa ttgctgagtt aagcttttga gttaaatttg
    61441 tactaccttt aggagtaatg aaataaaatg gttttgatga aaattttgct acagtacgta
    61501 caccttctag tgggttcata ctaacattaa gaagtacatc tgctttacca ctctttagtg
    61561 cttctagttg ctcatcattt gttttacaat ataccaattc aggtgacata agattcattt
    61621 tacaatactc atctaactct ttaagtctag tatcagaatt ttctattaca gcaatacgga
    61681 tttttttcac atcttgtgaa ctcattatgt tatcaatatc tgtattttca tataatgctt
    61741 gtagtactgt atttgctact ccataactat ttcctgcata attatatatt ttttgcattg
    61801 gctcattgta taacatacct cccattatat caatttcacc attttctaac atcttcatta
    61861 attttgtaat agattaattt atctctccag gaacttgaac aaattcataa ttccatccag
    61921 tatactgtgc aatttctagt aaatattcat atgtataccc aaaatagtta ccttgctcat
    61981 ctatttcagt taaacctttt tgaataggat aagcaacacg aatggtacga ttatcattac
    62041 ctatagcgtt gataccaaaa gaaaatatgc aagaagtgaa tatagatgta attacaataa
    62101 ttaataaaag taattttttt aaattcacta cacaatcctc catatagtta agatttacag
    62161 tataaattac tgcataaact tgtagacatg taaaaataga ttagtatata aaaggatata
    62221 taaatctact tagaaattta aatatcctta atatatacaa tatatttaaa ttataactca
    62281 tttatatgtc tattacaaca aatttaccct gctaaatacc tactctatat cccataattt
    62341 tacaagttgt agtgaagttt aagttctatc tcacctcaaa tttagcagtg tttctattta
    62401 cagttctttt atatttcaca tctggataac ccaatataaa tgaagtgata acagcttcgt
    62461 tctcgctcat tcctaacagt tcttttatct ttggctctac atttgatgcc ataacaaaaa
    62521 atccattata acaaattcct aatccttgag aatatgccat aagttccata ttagatgcag
    62581 ccagtcctcc atctacataa gcacttccac ctagagacat atctcctaca actaccataa
    62641 gtagtggggc atcataaaat aaagaatcat ttccgttctc tttatatctt ttgtacattt
    62701 tcttaaatgt atttttatag atagaacgta cagggtcatt gtcatcagta tctaatgcca
    62761 aattataaag accttgtatt gccagttctt tcacttcttt tagcttttct tttactaata
    62821 tgtatcttat aggctgtcta ttaccacctg ttggtgtgta tctaccagct tccactatgt
    62881 tttttatttt ttcttcttct acctctatat ctttatattg tcttatactt cttctaaatt
    62941 taataaaatt caataacttt tcgggttcta attcaaaact ttcacgattg tattctatta
    63001 cttcattttt atcaaatcct tgcatttcta ttgcatctgt tgggcaaatt gccatacagt
    63061 gaccacagtt aatacagaga atatttttaa cctttgcttt atcatcctta acttttattg
    63121 catttacaag acagtctgat tcacacatcc cacagcctac acatttttct aaatttatgt
    63181 ctatcattcc aaattattcc tccttttaaa aaatttactt tagtataaaa caaatagttg
    63241 tctaagtcaa tttttatttt tttaacaata tactagcctt aactacactt taatggagaa
    63301 aaatatacat aaattggaac ttaaattaga acttattcaa agtaaaagat aaaagaagta
    63361 gatgtctaaa agtgaacttt ttagttaatt agatattctt tattgtatga aatcaagtgg
    63421 atatttatca tttcaacaat aaaaaaggct tacctctatt ttggatatgt ccctgtcaag
    63481 tagacagact aaaaaaaggc tatctttatg cactgagata gccccttttc ccaactacta
    63541 ttgtttatct taatattagt caataaattt aatttgctat aacctgtaag ttacatttta
    63601 atttgatgta actttaaatt acatttttaa gtaatatctt taaattatat tcttaaaata
    63661 tatcattaaa ctacatttta attaatctaa aaactatatc ttaattaatc taagaaactt
    63721 acattataat cttttagttt cccattatca tatacaatct ttgcactaaa gatatttcca
    63781 gtcttagccc aatttaagaa cagcctgtca ccttcccaca tatttaaatc gaatatttta
    63841 tcattatcaa cccaaattaa atttccttct ttacaatcat ttaaatcttt ttcagataat
    63901 tcaccttcaa actcatctga gaaatacaaa aacatatacc aatcttcttc atctttaaat
    63961 tttggaaaag ttatcaaccc tttatatgac aatgatttaa gagtaagacc agtctcttct
    64021 tttacttctc taattacaca ttcttctgga gtctctcctt gctcaaactt tcctccaact
    64081 cctacatact taccttcatg tatatcatct ttctttttat ttctgtacaa catcaaagtc
    64141 ttgtcatctt tttctatata acaaatagtt gtaagtttca tgtatctcgc cttgctttct
    64201 ctttatattc tcaactaatt attttcagca ctttcaagca catcacttat attcttcatt
    64261 gcattaagag aaagctctgc aaattcatca aatggaatcc ctatatcttc tatacttctc
    64321 attgcatctc tatttacatt tgaagcaaat ctcgtttctt tcattctttt aataacagac
    64381 ttagtcttta cactggtaag ttttttatct ggataaacta aagctatagc agttataaac
    64441 ccagttatag ggtctgctgc atatattgct ttttccatta ggctagttct tgtaactccc
    64501 attccatcat gatgaccaag tattgctcta tacatttctt cgtcaccgaa gttttctttt
    64561 ttaagcagtt ctactgttgt tgtagcatgt ccctcacata tttctcctgg ctttcttcca
    64621 actatatctg aatccaaatc atgtaaaaga cctgccactg cccactcttc ttctttttct
    64681 ggctctagtc ttttggctaa ttctctcata acagcttcaa cagcataaca atgttttctc
    64741 attctatcag tttctaaata cttaaataaa atttctaaag cttcttctct cattttcacc
    64801 aatccccctg ttttttcaca tatatgatat ttttatatat taatacacat aaattcaagt
    64861 ttttatatat attcatgtat atttttcatg ttatatctat tctacacctt tatagtagtg
    64921 tcctgcaact gttccaattt catctaattt cttatacaag tctgtagccc tttttccaag
    64981 tttataatta ttactcaata cctcaatgtg agcttcaata acctttttaa ccatctcact
    65041 atcttcaact gtaaaatcta tcctaaatac atctattcca atttcctcta actcatatag
    65101 attgtccaac atacaagtaa ccttcgaatt gtaaattgta cttctacaga atatatcttg
    65161 tgttatccta taacttgcat ccgtaaagtc tcttaaagca taactacttt ccttacattt
    65221 agcaactctc ttgtcctttt tacaatccct aacaactact cccataggac agtattctgt
    65281 aaccatcata ggagcatatc catatattac tgcttcaatt tctacatctg tatattttaa
    65341 agtttgttta atttcattta agtttagttc ttgtgacaaa caaactgtac ttgctccctc
    65401 ttctttgaag tggtttatac tttcactatt gaatgcattt aagtagcaat ctatatttaa
    65461 gtcttttttc ttaaagtatt ccatagagcc ccagttacca atttgaacct tattaattcc
    65521 ttttatatta tccaatatct caaattgctt gtactcttta tttctaacaa tccttggtgc
    65581 tgaatagatt aattttttgt cagtgactgc tgtattagag tggttcttat tcaaagtatc
    65641 tattttactt gtatttgaca aagtatatat gttatttgta tttgaaccca ttagttcttt
    65701 aggaatacct aaagatgaag aagtatataa atccatagaa atacttgaat ctatagaaat
    65761 acttgagacc atttgtaaag cttttgataa tgtatttata tcttcataat agattaaatc
    65821 tacattataa tttaatactg cttctaattg ctccaagttc ttaactttaa ctctaatctt
    65881 aggaactgta tcaagttttt tctgtttatt tactctattt ggctggtatt ttaatctatt
    65941 cttcttaaat tttctatctt ttatctttat tctttcttca tctaaaagct caatacaatc
    66001 ccttctcatc tgatttaaca cacttatagg catacttaca ccttcatcca ggtcaatttc
    66061 tatctctttt aacttatatg gtgtatttcc aagcttttga atttgtgttt ctattttttc
    66121 tttacttaaa gccaccttta tagctttttc aactattttg tctccttcta ccaccgattt
    66181 atttccacaa aaatcactta taaaaagctc tggcttttta cctagtttaa tactcacttt
    66241 tgcacgtata ttagttttta tgaactcttt atcttgctga aatgtagctt gtactctatc
    66301 catcaattcg ctgtcagaag tcttatatat aagctgattt ctctttgctt ctccaacaaa
    66361 atctaattct atcaaatccc ctttatgtgc tattgtagtt atctctccat cctttattat
    66421 tcttccaata gtacctccac ctaagttaat tccatcacct ttcttaagcg tattttctag
    66481 cattactcta agtcttttgt tctttctatt ataatctaaa acttttccaa tatatagccc
    66541 ttgattatta ggtctattag agttcattat atccttacca acttcaccta atatatatcc
    66601 ttttgtaaac tttctattaa atatggtata caaattattt attatttcct cagaaatact
    66661 tatttttttc ttatctacaa attcattaat cgcttctcta tatcctgaaa ccacagtagc
    66721 tacatactct ggctttttca ttctaccttc aatttttaaa gataatacac ctgcttcaat
    66781 tattttatcc aaatcttcta tagtacttaa atctctagga ctcaacaaat aatctccatt
    66841 actttttact acttctcctg tatttatatc tatcaactca tacttttgtc tacatggctg
    66901 tgcacaccta cctctatttc ccgacctatt gcctatcata ctactcataa ggcattgacc
    66961 cgagtaacat acacataaag ctccatgtac aaaaacttct atatctacag ttgtattttc
    67021 acaaatccat tttatctcat caacatttaa ttctcttgct aaaacaactc tatcaaaccc
    67081 tacactctga aggtatttta catcttctaa ggaatgtgct accatttgag tgcttgcatg
    67141 tagttcaaag tcaggtaata gtttttttat aagtcttgcc attcctatat cctgtactat
    67201 taaagcatca atatctatat cgtataaaaa ttttacatac tctacaaaat cttctatttc
    67261 atactgtttt atcaatgtat ttacagtaac aaagacttgc actcccctta tatgagagta
    67321 tttaacagcc tcaatcagct catttctatc aaagttattt gcagatgctc tagcactaaa
    67381 gtctttacca cctaaataca cagcatttgc tccgttttga actgctgctt ttaatgaatc
    67441 aaaagagcct actggtgcaa gtaattctat ttttttcata tttttctcct ctatttatac
    67501 ttgttaggtt aatataatta tatctataac ttaaatattt gtattaaatt tatatatttc
    67561 ttactatatt
//
LOCUS       DAELTA010000004        65973 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.004, whole genome shotgun sequence.
ACCESSION   DAELTA010000004 DAELTA010000000
VERSION     DAELTA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.004"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            219..674
                     /locus_tag="KPG13_000193"
     CDS             219..674
                     /locus_tag="KPG13_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG0983941.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            723..1307
                     /locus_tag="KPG13_000194"
     CDS             723..1307
                     /locus_tag="KPG13_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG0983942.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1312..1635)
                     /locus_tag="KPG13_000195"
     CDS             complement(1312..1635)
                     /locus_tag="KPG13_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0983943.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1787..2305
                     /locus_tag="KPG13_000196"
     CDS             1787..2305
                     /locus_tag="KPG13_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0983944.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2481..3149)
                     /locus_tag="KPG13_000197"
     CDS             complement(2481..3149)
                     /locus_tag="KPG13_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983945.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3264..4679
                     /locus_tag="KPG13_000198"
     CDS             3264..4679
                     /locus_tag="KPG13_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0983946.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4902..5360
                     /locus_tag="KPG13_000199"
     CDS             4902..5360
                     /locus_tag="KPG13_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0983947.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5357..5941
                     /gene="clpP"
                     /locus_tag="KPG13_000200"
     CDS             5357..5941
                     /gene="clpP"
                     /locus_tag="KPG13_000200"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0983948.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6253..7329
                     /locus_tag="KPG13_000201"
     CDS             6253..7329
                     /locus_tag="KPG13_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0983949.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7396..9471
                     /gene="bclA3"
                     /locus_tag="KPG13_000202"
     CDS             7396..9471
                     /gene="bclA3"
                     /locus_tag="KPG13_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG0983950.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            9640..10401
                     /locus_tag="KPG13_000203"
     CDS             9640..10401
                     /locus_tag="KPG13_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0983951.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10710..10988
                     /locus_tag="KPG13_000204"
     CDS             10710..10988
                     /locus_tag="KPG13_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983952.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11281..11856
                     /locus_tag="KPG13_000205"
     CDS             11281..11856
                     /locus_tag="KPG13_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG0983953.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12295..13005
                     /locus_tag="KPG13_000206"
     CDS             12295..13005
                     /locus_tag="KPG13_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0983954.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12999..14315
                     /locus_tag="KPG13_000207"
     CDS             12999..14315
                     /locus_tag="KPG13_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0983955.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            14327..14467
                     /locus_tag="KPG13_000208"
     CDS             14327..14467
                     /locus_tag="KPG13_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0983956.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            14526..15107
                     /locus_tag="KPG13_000209"
     CDS             14526..15107
                     /locus_tag="KPG13_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0983957.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            15254..16963
                     /locus_tag="KPG13_000210"
     CDS             15254..16963
                     /locus_tag="KPG13_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983958.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            16978..17850
                     /locus_tag="KPG13_000211"
     CDS             16978..17850
                     /locus_tag="KPG13_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0983959.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            17851..18639
                     /locus_tag="KPG13_000212"
     CDS             17851..18639
                     /locus_tag="KPG13_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983960.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(18735..19568)
                     /locus_tag="KPG13_000213"
     CDS             complement(18735..19568)
                     /locus_tag="KPG13_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG0983961.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(19636..20265)
                     /locus_tag="KPG13_000214"
     CDS             complement(19636..20265)
                     /locus_tag="KPG13_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG0983962.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            20575..21234
                     /gene="cprR"
                     /locus_tag="KPG13_000215"
     CDS             20575..21234
                     /gene="cprR"
                     /locus_tag="KPG13_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG0983963.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            21445..22503
                     /locus_tag="KPG13_000216"
     CDS             21445..22503
                     /locus_tag="KPG13_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0983964.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            22754..24898
                     /locus_tag="KPG13_000217"
     CDS             22754..24898
                     /locus_tag="KPG13_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:23168..23170,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBG0983965.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            24947..25780
                     /gene="fdhD"
                     /locus_tag="KPG13_000218"
     CDS             24947..25780
                     /gene="fdhD"
                     /locus_tag="KPG13_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG0983966.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            25791..25964
                     /locus_tag="KPG13_000219"
     CDS             25791..25964
                     /locus_tag="KPG13_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983967.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            26022..26573
                     /locus_tag="KPG13_000220"
     CDS             26022..26573
                     /locus_tag="KPG13_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0983968.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            26598..27974
                     /locus_tag="KPG13_000221"
     CDS             26598..27974
                     /locus_tag="KPG13_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0983969.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            27974..28489
                     /locus_tag="KPG13_000222"
     CDS             27974..28489
                     /locus_tag="KPG13_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0983970.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(28567..29850)
                     /locus_tag="KPG13_000223"
     CDS             complement(28567..29850)
                     /locus_tag="KPG13_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG0983971.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            30038..30460
                     /locus_tag="KPG13_000224"
     CDS             30038..30460
                     /locus_tag="KPG13_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG0983972.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            30562..31500
                     /locus_tag="KPG13_000225"
     CDS             30562..31500
                     /locus_tag="KPG13_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG0983973.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            31686..33710
                     /gene="ligA"
                     /locus_tag="KPG13_000226"
     CDS             31686..33710
                     /gene="ligA"
                     /locus_tag="KPG13_000226"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG0983974.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            33870..35219
                     /gene="rph"
                     /locus_tag="KPG13_000227"
     CDS             33870..35219
                     /gene="rph"
                     /locus_tag="KPG13_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG0983975.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            35219..35689
                     /locus_tag="KPG13_000228"
     CDS             35219..35689
                     /locus_tag="KPG13_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0983976.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            35883..37169
                     /locus_tag="KPG13_000229"
     CDS             35883..37169
                     /locus_tag="KPG13_000229"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG0983977.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            37319..37903
                     /gene="clpP"
                     /locus_tag="KPG13_000230"
     CDS             37319..37903
                     /gene="clpP"
                     /locus_tag="KPG13_000230"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG0983978.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            37922..39172
                     /gene="clpX"
                     /locus_tag="KPG13_000231"
     CDS             37922..39172
                     /gene="clpX"
                     /locus_tag="KPG13_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG0983979.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(39243..39770)
                     /locus_tag="KPG13_000232"
     CDS             complement(39243..39770)
                     /locus_tag="KPG13_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0983980.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(39758..40270)
                     /locus_tag="KPG13_000233"
     CDS             complement(39758..40270)
                     /locus_tag="KPG13_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0983981.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            40450..42813
                     /gene="lon"
                     /locus_tag="KPG13_000234"
     CDS             40450..42813
                     /gene="lon"
                     /locus_tag="KPG13_000234"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG0983982.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            42803..43414
                     /locus_tag="KPG13_000235"
     CDS             42803..43414
                     /locus_tag="KPG13_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG0983983.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(43679..45049)
                     /locus_tag="KPG13_000236"
     CDS             complement(43679..45049)
                     /locus_tag="KPG13_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0983984.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(45224..45339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45627..46706
                     /locus_tag="KPG13_000237"
     CDS             45627..46706
                     /locus_tag="KPG13_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0983985.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            47097..47777
                     /locus_tag="KPG13_000238"
     CDS             47097..47777
                     /locus_tag="KPG13_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0983986.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            47770..49149
                     /locus_tag="KPG13_000239"
     CDS             47770..49149
                     /locus_tag="KPG13_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG0983987.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            49320..50405
                     /locus_tag="KPG13_000240"
     CDS             49320..50405
                     /locus_tag="KPG13_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG0983988.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            50459..50818
                     /locus_tag="KPG13_000241"
     CDS             50459..50818
                     /locus_tag="KPG13_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG0983989.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTINSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            50871..51743
                     /locus_tag="KPG13_000242"
     CDS             50871..51743
                     /locus_tag="KPG13_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG0983990.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            51744..52187
                     /locus_tag="KPG13_000243"
     CDS             51744..52187
                     /locus_tag="KPG13_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983991.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            52202..52786
                     /locus_tag="KPG13_000244"
     CDS             52202..52786
                     /locus_tag="KPG13_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983992.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            53039..54076
                     /locus_tag="KPG13_000245"
     CDS             53039..54076
                     /locus_tag="KPG13_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0983993.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            54157..54522
                     /locus_tag="KPG13_000246"
     CDS             54157..54522
                     /locus_tag="KPG13_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983994.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            54527..54844
                     /locus_tag="KPG13_000247"
     CDS             54527..54844
                     /locus_tag="KPG13_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983995.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            54892..56496
                     /locus_tag="KPG13_000248"
     CDS             54892..56496
                     /locus_tag="KPG13_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0983996.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            56620..58248
                     /locus_tag="KPG13_000249"
     CDS             56620..58248
                     /locus_tag="KPG13_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0983997.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            58513..59862
                     /locus_tag="KPG13_000250"
     CDS             58513..59862
                     /locus_tag="KPG13_000250"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG0983998.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            60018..61097
                     /locus_tag="KPG13_000251"
     CDS             60018..61097
                     /locus_tag="KPG13_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0983999.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            61442..62350
                     /locus_tag="KPG13_000252"
     CDS             61442..62350
                     /locus_tag="KPG13_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0984000.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            62355..64724
                     /locus_tag="KPG13_000253"
     CDS             62355..64724
                     /locus_tag="KPG13_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG0984001.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            65049..65852
                     /gene="proC"
                     /locus_tag="KPG13_000254"
     CDS             65049..65852
                     /gene="proC"
                     /locus_tag="KPG13_000254"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0984002.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        26679 a         7145 c        11640 g        20509 t
ORIGIN      
        1 tttcaatgat atattaaggt tctaatatat tatacaaaat aaaaacatta attcaactta
       61 aataatttta agtattatat gtgttttgat taaagaaaac taaaatttta aggaaaaata
      121 acatatttgt attaaatttt attgaaatga ttaaatttat ttacttaatg taaattatta
      181 gctaaaattg tattaaataa ttaaggaggt gagtagctct ggatgaaata gatttaaaaa
      241 taattaattc tctaaaaaag aatagtaggg cttcaacctc tgaaataagc agacaaatta
      301 atttatcagt accagcagtc tcagagcgta taaggaaaat tgagcagtca aatttaattc
      361 aaaagtacac tattcaaatt aatcgggaaa aaacaaaata taagcttttg gcttttatat
      421 ttatcaatac tgatttatct aaaagtatga aagaatttaa gaaaactata ttggaatatg
      481 atactgtatt agaatgtcat catatttcag gagagtatga ttacttaata aaagtattag
      541 cagaagatac aagtaatctt gaagagttta tatcagtgac tttgaaggaa attgggggga
      601 ttcaaaaagc taatacaacc attgttttat ctacaattaa agaagaattt aatacaataa
      661 aagatatttt ttaaataatt tactagtaaa gtgttaatta tattttagga ggaaaataga
      721 atatgaaagt tttagttttt ttagcaaaag gttttgaaac tatggaattt agtgtatttg
      781 tagatgttat gggatgggct cgtaacgatt atgggcatga tattgatgta gtgacttgtg
      841 gatttaaaaa acaagttatg agcacattta atatacaagt attagttgat aagacgatag
      901 aagaagtatg tgttgatgat tatgatgctc tagctattcc tgggggattt gaagaatttg
      961 gattttatga tgaagcatat gattcatctt ttttaaatct aataagggag tttaattcaa
     1021 aagaaaaaat aatcgcttct atttgtgtgg cagcattgcc agttggaaaa agtggtgttt
     1081 taaaaaatcg taaagctact acatatcatt tgaaaaatgg taaaagacaa agacagttaa
     1141 gtgagtttga tgtaaatgta gttaatgaac caattgttgt agataaaaat attataacct
     1201 catattgtcc agaaacagct ccacatgtag cttttaagct tttagaaatg ttgacatcta
     1261 aagaacaaat ggatgaagtt aaattagcaa tgggatttaa actgtaatgg tttatagatt
     1321 ttctaagtaa tcagaacccc agtcacacat ggaatctaaa attggaataa tactctttcc
     1381 aaaatcagat agcgagtatt ctacttttgg aggaacaaca ggataaacca ctcttataat
     1441 taaaccatct gattctaatt cacgtaattg ttgagttaac atcttaggcg ttgctttagg
     1501 aattaatttt tttaattcat tgaacctcaa tatagtgtct ttaagatgcc aaagtataag
     1561 tgtcttatat ttaccaccaa tcaatgcaag tgttgcttct attgggcaac tgtaattatc
     1621 taaacatttt ttcatttttg ggctccttta tttattttaa agtatctttt taggtactat
     1681 attacaaaaa agtgcattat tgtaatatag taccatagtg ataaaattat agcataggtt
     1741 aaataaatca tcaacttagc ctaataaaac aataggaggt aaataaatga atttcgttga
     1801 acttgcaaaa aaaagatatt cttgccgtaa ttatcaagat agaaaggttg aaaaagaaaa
     1861 acttgagaag gttttagatg ttgcaagaat agctccaact ggtggtaatc gtcaacctca
     1921 aagattgatt gttattcagg aaaaagaagg tataaataaa ctttctaagg ctgccaatat
     1981 atatgatgct ccattagcaa ttcttgtatg tggagataaa gacaaagttt ggacaagacc
     2041 ttttgatggg aaacaactta cagatattga cacaagtata gttacagacc atatgatgtt
     2101 acaggcaact gaacttggat tggcaagtgt ttgggtgtgc tattttaatc cagatataat
     2161 tagagaagag tttagtttgc cagataatct agaaccaata aatatattat tgatgggata
     2221 tgaatctaag ataccagaga gcccagagag acatgaaaaa actcgtgttc ctttaagtga
     2281 aattgtttct tatgaaactc tttaattata aaattaagtt attcatagga taaatgtaat
     2341 aaagtgttta ttataaacaa aattcaaaat aaataatatc tacatataat taattttagt
     2401 gaagtatatc tagtttaact agaatgtata cttcactaat ttatttattc aaaatattta
     2461 tgaggatttt aatatattaa ctaaaatata tcaattgaat taacttttaa atctggaaaa
     2521 gcacttgaaa tatagtgttt tatgtcattt aaatggtctt gaatttgctt ttctgatatt
     2581 ttatcaggtc catatacaaa atacataata tttttagtat cttttgttat agctgttcga
     2641 ttatcagggc cattaagaat actagataat ataccaatat tatctgataa aaataaatca
     2701 tcttttgtta aaatatgttc tttaccacct ataccataaa aatgttcact tccatttgct
     2761 aattctattt tcaaaggtaa ttccatttta tcaaggtcat gacctgctgt tagaagaaga
     2821 tttttcaatt ctgctaaaaa cattgactca attgttacac ctacatctgg gatacctttt
     2881 gattttaata gaatagattc taattgaagc aatacagggt aagttttctt gaatttttta
     2941 taatacttta tataacttga aacaggctct gttttaattt tttctttacg attgtattct
     3001 gggtgttgtt ctataatatt tttaattgta gagtttttaa gaactaaaaa attttcttta
     3061 ttaggttttg aatgtgtaat atttataacc ataactccaa atttaatatt gggataaatt
     3121 tctttaactt tatgtgatat ttctaacata atatagactc ctttttattt tatattttat
     3181 gatataatct tagcaaatta aaagtggatt ttgtagttcc atttttatag taattttata
     3241 gaaccacttt tggaggtatt agaatgtgga ttacgattaa tagaaatagt aatatatcat
     3301 tatcaagaca aatttattgt gagataaaag atatgattct ggatggaagc ttaaagtttg
     3361 agcaaaaact gccatcatct aggactttat caaaagaact atctgtatct cgtaatacag
     3421 ttttagatgc atataatcaa ttaattgcag aaggatattt ggaaacaagg aatggatatg
     3481 gaacaattgt agctaaagga atatcagggc ataaagttga ttttaagtta aatgatactt
     3541 ttagagatga aaaagattta catactaata aacaaaaaaa ttatatagat tttcgttcag
     3601 gagttccaga attaaaacta tttccacgca aagaactttc aaagctatat aaacaaatac
     3661 tgtatgattt atcagattcg gatttcagat atggaagtac agctggtgta tgggttttaa
     3721 gagtggaaat atctaagtat ttatttcgta ctagaggaat tagatgtaat cccaaaaaca
     3781 ttatgattgt ttctggttca acacaaggtt tatctttaat ttcacagctt ttatacaaaa
     3841 gtaataaaaa ggttgctgta gaagacccta ttcataaagg cttattaaag gttatttcat
     3901 ctattggata ttcagttgtt ggaattaaag tagatgataa gggaatgaat accaacttaa
     3961 taaaatcaat aaaggggatt tcttttgttt atactacacc ttctcatcaa tacccattag
     4021 gagggatttt acctattcaa agaagactag agcttattaa atgtgcaaca gagaatgatt
     4081 actatataat tgaagatgat tatgatagtg aatttagata tgagggtcaa ccaattagtt
     4141 ctctttatga acttagtcca aataaagtaa tttacatagg ttcttttagt aaaatattat
     4201 ctccagctat tcgtgttgga tttatgttat taccagacaa tattttagaa agctataaaa
     4261 ccttaaaaat gtactctgat gttcatacag aaacaatatt gcaacatgta ttagctgaat
     4321 ttattggaaa tggaggttta gagaagtaca tatttagaat gaagaagttg tacaatagaa
     4381 aacgtaagta cctcataaaa gttttaaaca cggaatttaa aaatgaattt aaaataaaag
     4441 gtgaagcagc agggcttcat atagttgctc atttctataa tgttaaattt acggaaagtt
     4501 taattcataa aatttttgaa gcaggtataa aagtttatcc tatacaacaa tatgcaattg
     4561 agaatatatt agaatatgac tatgaaatta tactaggtta tgctcatctt agttttgatg
     4621 aaattgaaaa aggtgtaaaa atactcagca aaataataca tatgaatcaa ggtacataga
     4681 taaaacatgt agcaatctac atataataga tgtaattaag tttaaacttt aaaagaaata
     4741 tatattaata tttttgtata aaaatacctg atattttttt atcgatactt tattgctata
     4801 cactaaatta agatagcaat ttatattaag aaaaaacttt ttgcataggt tataatactt
     4861 aaaaggagag gatagagttg aaaacaaata cagatgttat tatggagata attgattata
     4921 tagaagaaaa tcttacagaa aatctaaatt tagataaaat agcttatatt gctaattatt
     4981 caaaatacca tttacacaga atgtttacaa atgttgtagg atgtactatc catcaataca
     5041 tacagaggag gagacttact gaagcagcta gacaacttat atttacagac aaatatatta
     5101 tagacatagc tattacagta ggatattcta cacatcaatc ttttacacta gcatttagaa
     5161 atttatataa agaatctcca caagtatata gaaataaaga tgaattttat cctattcaac
     5221 agagatttgt tttaaatcta aatcatcaaa ttcaaagtga aaaatgtatt tcttatgcaa
     5281 atatgaatta taaacaaaga agagatgaaa tcaattctta tctgaacttt aatattaatt
     5341 gtgaggtgaa agtagcatga gtttagtgcc acatgtaatt gaacaaacag gtcaagggga
     5401 aaggtcttat gatatatatt caaaattatt aaaagataga attatattta taggagaaga
     5461 aattaatgat gctattgcaa gtttagtagt agctcaatta ctgtttttag aatcagaaga
     5521 cccagattca gatataatta tatatataaa tagccctggt ggctctgcta catcaggatt
     5581 tgctatatat gatactatga attatgtcag atgtgatgta tcgactattt gtattggtat
     5641 ggctgcaagc atgagtgctt ttttacttgc aggaggaaca catggaaaaa gatgtgcttt
     5701 acccaattca gagattatga ttcatcagcc aatgggagga gctaaaggtc aagcaacaga
     5761 tgtaaaaata gctgtagata atatattgaa aataaaagaa cgattagata aaattctttc
     5821 tgaaaataca ggtaaatcaa tagaagatat taggagagat acagatagag ataattttat
     5881 gactgcacta gaggctaaag agtacgggct gattgattat ataatgaata ggaatgaata
     5941 aagtatcaaa gtatcatatt aaaatgtctg ttttacaaat atatatataa tattaaataa
     6001 gagtgtctca aaatgaactt tttagtttat gggacactct tttttgtatg aaattaaata
     6061 tggttttatc atttcgacaa ttattaccaa atttcaagtt taataaattc tactataaaa
     6121 aatacataaa agataaggtt ctttagtaac tttctgtcgt caaaagtttt tataaattta
     6181 ataacaaaac aagagaaaat accatatatt acttatgtct gcaatatata gaagacaaag
     6241 gagttgtaca ttatgattac aataagtttg tgcatgattg ttaagaatga ggaagaagtt
     6301 ataggcagat gcttagaatg tgtaaaggat atagtagacg aaattataat tgtagatacc
     6361 ggttctacag attccacaaa agaaatagtc tcaaaatata cagacatggt gtacgatttc
     6421 gaatggatag atgatttctc tgctgcaaga aatttttcat tttctaaagc ttctaaagac
     6481 tatactatgt ggcttgatgc agatgacata attttagaag ctgatagaga aaaattatta
     6541 aagattaaag aaagcttgga tacttccatt gatattgtta tggcaaaata caatgtatcc
     6601 tttgatgaaa atggtaaccc aactctctca tattatagag aacgattgtt taaaagaagt
     6661 tctaatcata aatgggttga ccctattcat gaagtagtac ctctttttgg agaggtattt
     6721 tattctgaca ttgcaatatc tcataaaaag cttcatagac aggaccctct aagaaatctt
     6781 agaatttttg aaaaaatgat ttcagaagga aaaacattgg agccaagaca ccaattctat
     6841 tattctagag agttgtacta taatgctagg tataaagaag caatagaagg ttttactaag
     6901 tttttagact catcaagagg atggattgag gattgtatca gtgcttgcag agatttagcc
     6961 acatgttatt acttaattaa tgatgaaaaa agtgccttat attcattgtt tagaagtttc
     7021 gaatttgatg agccaagagc agaaatttgt tgtgacatag gaaagcatat gtttgataga
     7081 caaaaatata aagaagccat tttttggtat aaggtagcat taacaagaga taagaatgat
     7141 actgatggag gctttaagtc agatgattgt tatggatata taccatctat acaattaagt
     7201 gtctgttatg acaggttagg agaatcagat aaagctattt attatcatga aaaaacaaaa
     7261 gaaatcaaac ctaatgatag tgctgttttg cacaatgaaa attattttag taaatttaaa
     7321 aatagttgat gggtcacaat catactaaat ttgacatatt atatatagag cttaaaagct
     7381 caaatatatc aggaggtgct tttaataatg agtagaaata aatattttgg accatttgat
     7441 gataatgatt acaacaatgg ctatgataaa tatgatgatt gtaacaatgg tcgtgatgat
     7501 tataatagct gtgattgcca tcattgctgt ccaccatcat gtgtaggtcc aacaggccca
     7561 atgggtccaa gaggtagaac cggcccaaca ggtccaacgg gtccaacagg tccaggtgta
     7621 ggggcaacag gcccaacagg accaaccggt ccgactggtc caacaggaaa tacagggaat
     7681 acaggagcaa caggattaag aggtccaaca ggagcaacag gggcaacagg cccaacagga
     7741 gcgacaggag ctataggatt tggagtaaca ggtccaacag gcccaacagg agcgacagga
     7801 gcaacaggag cagatggagt aacaggtcca acaggtccaa cgggagcaac aggagcagat
     7861 ggaataacag gtccaacagg agcaacaggg gcaacaggat ttggagtaac aggtccaaca
     7921 ggcccaacag gagcaacagg agtaggagta acaggagcaa caggattaat aggtccaaca
     7981 ggagcaacag gaacacctgg agcgacaggc ccaacaggag cagtaggagc aacaggaata
     8041 ggaataacag gtccaacagg tccaacagga gcaacaggag cagacggagc aacaggagta
     8101 acaggtccaa caggggcaac aggggcaaca ggagcagatg gagtaacagg tccaacaggg
     8161 gcaacaggag caacaggatt tggagtgaca ggcccaacag gtccaacagg agcaacagga
     8221 ataggaataa caggagcaac agggttaata ggtccaaccg gagcaacggg agcaacagga
     8281 gcaacaggcc caacaggagt aacaggggca acaggagcag caggactaat aggaccaacc
     8341 ggggcaacag gagcaaccgg agcagatgga gcaaccggag cgacaggggc aacaggagca
     8401 acaggtccaa caggagcaga tggattagta ggtccaacag gagcaacagg agcaacagga
     8461 gcagatggat tagtaggccc aacaggtcca acaggggcaa caggagtagg aataacagga
     8521 gcaacaggag caacaggagc gacaggtcca acaggggcag atggattagt aggtccaaca
     8581 ggagcgacgg gagcaacagg agcagatgga gtagcaggtc caacaggagc aacaggagca
     8641 acaggaaata caggagcaga tggagcaaca ggtccaacag gggcaacagg tccaacagga
     8701 gcagacggat tagtaggtcc aacaggagca acaggagcaa caggattagc aggagcaaca
     8761 ggagcaacag gcccaatagg agcaacaggt ccaacaggag cagatggagc aacaggggca
     8821 acaggagcaa caggtccaac aggggcagat ggattagtag gtccaacagg agcaaccgga
     8881 gcaaccggag cgaccggagc aacaggtcca acaggagcaa caggagcaac aggagcaaca
     8941 ggtccaacag gtgctagtgc aataatacct tttgcatcag gtataccact atcacttaca
     9001 actatagctg gaggattagt aggtacacca ggttttgttg gctttggtag ttcagctcca
     9061 ggattaagta tagttggtgg agtaatagac cttacaaacg cagcaggaac attgactaac
     9121 tttgcatttt caatgccaag agctggaaca ataacatcta tttcagcata cttcagtaca
     9181 acagcagcac tttcacttgt tggctcaaca gttacaatta cagcaacact ttaccaatct
     9241 actgcaccaa ataactcatt tacagctgta ccaggagcga cagttacact agctccacca
     9301 cttacaggaa tattatcagt tggttcaatt tctagtggaa ttgtaacagg attaaatata
     9361 gcagcaacag cagaaactcg attcttacta gtatttactg caacagcttc aggtctttca
     9421 ttagttaata ctgtagcagg atatgcaagt gcaggaattg caataaatta gattagataa
     9481 gaaaaggctt ctaatatcgt attgatatta ggagcttttt taagaatgat tgatgaacat
     9541 agttttaata ttaattagtg cgcatattat aaaatactat tatataatat aaaataataa
     9601 cattaaattg gaaatataga attatctagg ggtgatacta tgaaaaataa gattgaaata
     9661 tcaatgaaca actttgtaaa aaattatcat aaatcaaata atataactac aaagtggcaa
     9721 gaacttttgt ttgcttatgc atctgctgaa gatgatatgt ttcagaagct aaaaagtgtt
     9781 gtaggaacat ctcatgctat gccaaaagat tttctggaaa atggaaaaac tataattaca
     9841 tattttatac cttttgatga aagtgtaaat aatagtaata ttggagggtt tgaatgttct
     9901 gaaatatggg caagagcgta catagaaact aatatgttga tttcaaattt aaatgatttc
     9961 ttatgtgaag aactagagaa gatgggggct aattcatatg ctattcctgg gtcgtataat
    10021 tttgacagtg aaaaattggt aagtgactgg tcacaaagac atgtagcatt catagcaggt
    10081 cttggaactt ttggtttaaa taatatgctt attacagaaa aagggtgttg tggaagaata
    10141 ggaagcataa tcacaagttt agaattagag ccaactaaaa gaccagaaaa tgaatactgc
    10201 ttgtataaac ataataatag ttgcaaaaaa tgtgtcaaca aatgcgtgaa agaagcttta
    10261 aaagaagatg gatttaatag gaataagtgt tatgaaatgt gtatgataaa tgaaaaaaaa
    10321 tacacttata taggtggatg tgatatttgt ggaaagtgta tagttggagt tccttgttca
    10381 gtaataaacc catcaagata atggatttaa gttgactaat aattttaaag atagtttata
    10441 atgaaaaaat tttagttatc aaaataaata taatataaaa actaaataaa tgtgtttaaa
    10501 aaatggataa ataatttgtg ttgattgaca accagttata ctgttgatag acttatatgt
    10561 atcgctgtga tatataagga gggttgagat tttatgtttg atttaatctt tatttttgca
    10621 tagcaagggc tattgcaact atgaaattta atatataaat agccctttgc tattcctaat
    10681 gaaaaattac tatttaatag gagagcaaaa tgagctataa aaaagcaata catatattac
    10741 cagaagaatt acttgaatta atacaggaat atgtagatgg tgaatgtatc tatattccta
    10801 gaaaatctaa taataaaaaa gaatggggaa gtaatacacc tactcgtgag gagttgtcaa
    10861 ttagaaatat gcagatttac gaagactatc aagttggata tgatttgcaa tatttatcgg
    10921 aaaagtatta tttatctttg aaaagtatcc aaagaattgt attacaagaa aaaagaaaag
    10981 atatctaagg atgagccagt atagttttat aattatactg gctcattttc ttttgataaa
    11041 agtgtttgag gtatagtttc ttttaagtgg atgatataat ttaagtaatt tcaataactt
    11101 atacagtagc taacaaagct ggttgtaaag tatctaataa gaagtagtag ctttttatat
    11161 gtttgaaatt aaaatttatt aaaattagga ggtcttgttt tgaaaaaaat acttgattat
    11221 attccacctt ttcataactt tctattaatt tagtaatctt atagaaagga aattgcattt
    11281 atgaaaaaaa ttacttggga ggtggaatat atctcccagt tacctgtaat taggtattgt
    11341 aaaaaatgtg gaaaaaagac tgaatatatt tgttcaggtt tatttagggt taatgctcaa
    11401 cataaatatt tggatatatg gttaatatac aagtgttcaa attgtgattc aacatggaac
    11461 tcaactatat attctcgtgt taatccaaaa agcttaaatc cagaaatact agaacaattc
    11521 catacaaacg attataatct tgttaagcaa tatgctatga atatggaact tttacataga
    11581 aatggagcag aagttggtat tccagagtat aaaatagtag gacaagaaat taatattaat
    11641 gaccttacac aagttcatat tattagtaaa tatccatgtc agttaaaggt atcaacatta
    11701 ttacgtgaga aactaggttt atctaggaag gtatttgacg aaatgttgga ttgtgggatt
    11761 attagaagta actcaggagt tgatttaaag aaaggtaaat taaattatga aattatgtta
    11821 gatataggca aggagaagca aaagtcttca aattgattag aataactaac ttaatatatt
    11881 ttcaggaaca tcatttaaaa aaatagtggc tttaaaaaat ttaaagccac tattttttaa
    11941 tatgttttat attaatattt aaaatatagt gtgtattctt caaaattata gcgataatta
    12001 aaatattact ataaaaagga aggtgcatac tttttattta acaaaaacta gatattaaaa
    12061 acttttcgag gaatatttta ttaaaatccc taaatatatt taatatatat taaaaaattg
    12121 tattataatg aaaatcaaaa tagattgcaa atccaaaaag taatacttaa tgtccaaaaa
    12181 gctaaaatta aaatctaaat agctatttac tacagaagta tgttaaaata tgtaaaatga
    12241 atagaaattt atatattttt attaaaaata tataagaaag gatgtttaag aattgtgatt
    12301 aatataggaa tatgtgatga tgaactacat tatcgaataa aaataaaaga tatattaagt
    12361 gaaatattaa gttcatatcc tataaattat aacatatatg aattttcagc aggagaagag
    12421 ttactaaata actatcctaa agatttagac atattaatta tggacataca aatgaaaaca
    12481 ataaatggta tggatactgc taggaagata agagagtttg atcataagtt agaaataata
    12541 tttgttactt catttgtaga gtttatgcaa gaaggttatg aggtaaaagc atacagatat
    12601 atactaaagc caataaataa agaaaaaatt tctaaaagtg tattaccttg tataaatgag
    12661 atgatgaaaa agagaaataa ttacttaact ataaatgtaa aaaattatgt agaccgaatt
    12721 aaaatagatt ctattactta tatagaaaca gatagaccga atatattgat ttatacacat
    12781 gatgatatgt atattactaa aatgagtata tctaaaatag aaaagatatt aaatgaatat
    12841 ggatttttta gatgtcataa tagctatata gttaacttaa aattagtaga atcaatgagt
    12901 ggaagtactg ttatagtaga tggaaaaagt attccaataa gtaaatatag ggttaaaggc
    12961 ttgaagcttg cgataactaa tatattaggg gacatagtat gctaacaaat ttaagtgtag
    13021 gattttggaa tattattaat ttagtttcag gtactataga ttggattata ttttttttag
    13081 taatccactt agttggaaag aggaaaatat ctaatataag atatatattt acaacaataa
    13141 ttataattat atttatggca tacataaatg tagttaaagt gtttccaaat attaagattc
    13201 ttctatgtat ggtaatagga atatcatttt atataacatc atatgaagat aaaatctata
    13261 aaagtataat agttagttta ttgttttggc taggattaat ggttgctgaa ggttctgcag
    13321 taggtctagt agtatttata aataaattaa ataatattaa cataacacaa catgaaaact
    13381 tatttagaat acaagctatt attatgtcaa aggtattttt atttataggt tttatattgt
    13441 ttaagtattt taaattatct ttagatttta aacctaaaga tatgatacta ataggaatac
    13501 ctatattatc taatatagtt agcttattac ttatatttga atataattta agagtcaata
    13561 caattactac agaaaactta tttatattta tatttgcaat tttattgatt ttatcatcaa
    13621 gtataatact tttaatagta atagggaaaa ttgtacaaga cgataaatta aatctagagt
    13681 atgaacttat aaatgaaaga ataaaaacaa actacaggaa ttatgaaaat ataaacgaag
    13741 ttcatgatag attaaaatat gtttatcatg acttgaaaaa ccatatgatt tgtataaaaa
    13801 attatgacac taaagaagaa attatatcat atataaataa cttagagttt cagataagtg
    13861 aatttgaaca ttttaaaaat actggaaata aaacattaga tttaatttta gcagaaaaaa
    13921 taagtatttg taaaaagtat gatattcaaa ttgaagataa cataaatata tctaaattaa
    13981 actttattaa aaataatgat atatgttcaa tatttgcaaa ttcactagat aatgcaattg
    14041 aagcatgtat ggatataaat aatgagatag aaaaaagaat agaagttaaa gctacatata
    14101 taaataaatt tgcaataata aaatttataa atactaaggt aaatgatatt aaacttattg
    14161 ataaacgtat tcaaactagt aaggatgatg ataagataca tggaatagga cttgctagta
    14221 ttaaatatat agtaaataaa tatggtggtg aaacaatagt taattactca gataatgagt
    14281 ttatattaaa aataatgata ccaataaaaa gttaggagag tacaaaatga aaaaaatagc
    14341 gttaaactta cttaaaaaca tctcagcatt aagttttggg atagctgtat tatcagcaaa
    14401 cagtgcaagt tcttgggtag cacatcaggc aaaagagcca caagctttac aaaaactaaa
    14461 aaaataacat taataaaagg acctcttctt attaaaggag aggttttaca tgttgaggag
    14521 gaaatatgtt taaaagatta tcatataaat ttgccaatat attagttaat aatgagattg
    14581 tagaaagtga agattttgaa atatatagat atggatttga aactttgatt tatttcatag
    14641 ttaatatttt ggtagcatta tttataggaa ttatttttga tagatttata catacagtta
    14701 tatttttaag ttgttattgc actttaagac agttcacagg aggttatcat gctaggaatt
    14761 ataaagagtg tacattaaca tttgcagtta tttatttgat aactattttt tcagctaata
    14821 atatagatat aaataaatat aagtatttat tagtattatt aatgattatt agtatactaa
    14881 caatttacaa gttagctcct ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa
    14941 agcattatag aaagactgtg caaaaaatac tattcgtaat aatatgttta attattttat
    15001 gtaaaatact taatattttt cagcaatatg tgatttatgc aataatttct atatattgga
    15061 ttgctatatt aatatatatt ggaatgaagg ttaatagtga tcagtaagat tataaaacat
    15121 tttctaaatt aattaacttc actaattaat attaacttta aaaaaataat gtcaaggtaa
    15181 gtatttgaac ttattaaata tagttgaatc tattgtaggt tgtatcaaaa gactggctag
    15241 gaggggcata aatatgagta gtattaaggc tatatcagac tttataacca aaataattag
    15301 aaaaaatatt ttatttgcaa taacaattat tattataaat taccatctca tacaacgatg
    15361 gacacatata tccaatagag gaatttcatt taatgattac ttatccttga gtgtatatac
    15421 ttgtatgaca attagtattt taaccatgtt aaatgtaatt ttttatatga gaaaaagtta
    15481 tgatactgta aaaatattat gtagaaataa ttttgattat tttttaggta tttctgttgc
    15541 tgtaactaga aacaacttta tattttgttg cataccaatt atatatgcta tagcatactc
    15601 aagtttaata agaacattgt caattgtaac gattaaatca actatattta tattgatatt
    15661 tgaatggttt ttaccactta ctattttatc tatagtgata tcatttattt cagttattat
    15721 aaataattta atactaaaga ttaccttatc atcagtattt ttatatttaa catcaactaa
    15781 tatgttggat agagttattg atattaaaga accatttttt aaagcattat ttaaatcact
    15841 taacatattt gaagatcaat catatgctta ctattctgat tttgtaggga aagttaataa
    15901 ttcagcatat gttatagata agattatacc tacactattt gcaattactt taataatagt
    15961 agcatatctg atattaaatg atattagaaa gaaacagatt aaatcattaa tagttatagt
    16021 agtgtacagt attttattta ttggattaaa ttatatatca atagatatgt caacatatag
    16081 agatgaggag cgtagttatg gagagtattt aaagaaatct aatattagtg atattaaaat
    16141 aaaatcgcat gatatgaaga ttgactttaa aagcaagtca aatataaaag atagaattgt
    16201 tttattaaac acatcaaatg aagagttaac aaatattaat atgattttag atgaaatatt
    16261 tgatataaaa tatataaaaa taaattcgga aaatataaaa tttaacaaaa aaaaggattt
    16321 aattgaagtt aatttaaagg aaagtttaga gcctaatgac gaggtgacat tagatataga
    16381 gtatgaaggc tatattaatg tgttgacatc ttggaatcaa aataaatata tagctactaa
    16441 agatgatatt atgcttccac caggttcttt agcgtggtac ccaaaagtaa atcaatcaag
    16501 ttgtatggct tataaaataa atttaagttc taaaaataat gtctattcaa atttaccttt
    16561 actgaataag actagtaatt ttttaaaaaa agattatata tttgaaggaa agtcaaatga
    16621 tattgcaata tatgcaggtg agtttaaaga agaggtaaaa gatgatataa aaataatcta
    16681 tccatcagat agtaatgtaa atatggatat agaggaaaaa attaaatttt taattgaaac
    16741 aagaaatgaa atattaggtg atagcaaagc atctgtagat gctaaaaaat atgtagatga
    16801 agtagataat gttatggaat caataaaaag aaaaaaaatt aatatgatat tagttgctga
    16861 cttacctagc aatgaatttt tatatgaaga ctcaaaggga gagaaaatta gcataactat
    16921 ttatcattgg ctttcaggaa atatccttat aatagataaa taataaagga ggttaatatg
    16981 ataaacaagt tagaaataag aaatctaaat tttaattaca aaaataaaaa agctttggat
    17041 aatataaatt taaccctaat agatggagta gttgcattat taggtcctaa tggtgctgga
    17101 aaatcaacat taatgaggtt attagtgact ctttatgaaa cttcaattgg agagatagaa
    17161 ttaaataata taaagtatag caaaaacaat gagaaaatca aggcaaatgt aggatatgta
    17221 cctcaagatt ttgatatgta taataatata aatgggcaag aatacttaga atttgtagct
    17281 aaaatgagag gtgtatctaa gaatgattta aagaaacata tacaaaaagt agtatcaaaa
    17341 gttaatctgg acaagtttat aaataaaaaa ataggtacat actcaggtgg tgtaaagaga
    17401 cgactaggta tagcacaggc tttaataggt gattctaagt taatagttat ggacgaacct
    17461 acagttggtc ttgatccaga gcaaaggaat gaatttagaa gattgttacc agaaataagt
    17521 aaaaattcta tagtattaat ttcaactcac atagtagaag acatacaatt taactgtgac
    17581 aaattaatta tattaaatca aggcaaaata ttatatgatg gtacaattaa taagtttatt
    17641 gatatggttg aggtgtattc ggtagtagtt tctaatgatg aatttgaata tttagaacgt
    17701 aatattgata ttattgatta taggagagtt aaagatggtg tgcaagtaaa gtatataggt
    17761 aaaaatacag agaaacttaa agatagctta aggatagata caactttaca agatgcttat
    17821 ataaactttt tacataagga gttaaaataa atgtgtatat tggagttaat aaaatttgag
    17881 cttaaaaaaa tagagcttaa acatgtgtta atatttgtaa ttatttctat cttatgcata
    17941 tttattacag gcatattaga ttataatgag ttaatatttt ataatgatgt acctataatg
    18001 gatacatatt ctatagcagg atggttagga attataaaga tatcagatgt ttttatacca
    18061 ataatgtcag caatcacaat aatttatgtg ttttcaaaag attattcggg taatataaat
    18121 gagatactta ctttatataa taaaaaaaaa tataatcaat ttatagtatt tagatggtgt
    18181 gttatattag ctatatatac tgtattatta ttaatttcaa ttacaatagt atatacattt
    18241 acgtcagtga aaggttttaa catagacaaa atagattttg tttatagtgt aaagccaata
    18301 gatgtgtttt taaagacatt tcctacatta ttatggtata caacatttcc actaatgata
    18361 ttaacattaa caaaaaatag atttacaaca atagcaatat taattacata tacatttgct
    18421 gatatatttt ttattttaca tatgtaccca tttgttagta tgattaatgt aaatggattt
    18481 tatattcaaa agatgtttta tatgcctaac aatatagatg ttagatttaa tgaattaaag
    18541 aattatttct ttataaatag aggttctcta gtagttattt ctataataat agtttcttat
    18601 atagcaagaa aatcaacggt tttaaaaaag agtaaatagt ttaatatatt ttttataatg
    18661 ttatatttat atagaatagt gactttaaaa aatttaaagc cactattttt taatatgttt
    18721 tatctatata actattattt ttctaggttt tttaatattt cgtaatagtg tgttgtggct
    18781 atttcacttg actcgccttt tttacaaaaa gataatttag atagttttgg aactacacgt
    18841 tcaacgacaa ttttattacc tatcatttta gattcaagta tcttcaatgc ttctaaaaat
    18901 ctattatcct tttttgcaca attataaaat gataaaacgt agacataaag gaataggtta
    18961 taattacgga aaggatattc aacttgcata aataaggttc caattccata gtgacaaggg
    19021 ccgattggtt ttttaatagt ccaatggtca agtagaaaat ctacagcttt gtcaagtttt
    19081 gattctttat ttagataatt actaaaacgg aatgcattta atatattaag agttggtagt
    19141 ggatttgagt attcagtttc tggacctcga ccaaaactaa atttattgca acgccaccca
    19201 ccatcagtat actgtgtatc taaaaaatgt tgaaacgttt tttgcattct aatgtcagat
    19261 gcatatccca tatgacagag tacgttagca gcatttgcag tatgacaagg gtaaatacct
    19321 ccatttggat atagtttgaa acgtccatct tctcgccatg tgctaaatat caagtcagca
    19381 cattctttaa gtaaagtatc atcagggttc acacctaatt ctaatagata aagtacacta
    19441 tcaagagtag agaaaggaga accttttatt aaacgcttat caggtgtagt ccaatagtct
    19501 gcaccattat catacctgtg tgataatatt atttcaatat ctgataaaga ttgattttct
    19561 acattcattt tttactccat ttcatattat attctattaa atctatcaaa aactttatta
    19621 taatattatt tatatttata attgatttat tttatttaaa attgcattta tagctgtatc
    19681 aggaacaact gctaaatctt ctgcattgaa gtaataaact atattagaat caaataatat
    19741 atttgaattt ggaggaaatt catcatctcc aaaccaaagt ataaatacca tagatgtatt
    19801 acccatatat gtaaacctat aagctaaatc tcccatgttt actttttcag ctccaatttt
    19861 ctccataact aacttatatt tgtctatttg agatgtaaaa acttctgaaa gcctatatag
    19921 agtcctgttt aaaaaatttg gataataaac attgcctcct ggtatttctt taaaagttac
    19981 atatttatta gttctaggaa tacctttagc attgattaaa tatctaacta tcaagatttt
    20041 aagctcaaag ttattgcaaa ggctaccatt actattccaa acttctccac ttggatagtc
    20101 tataatatat tgtctatcaa aaaaattgag tgtgaatcta tttaaatcat tattgaaagg
    20161 tatattactt aaattactaa ttgtatgagg gtctaattct ttgagttttt ttcgaacata
    20221 gtcaaatgga acattataat tattttttgt tatcttttca tccataattt tttcctccaa
    20281 tttaaacaat taaaagtata tttataaaaa gttttcttat tattaatttt attatatatt
    20341 caaaatatta caataataaa ttgttaaata aatttttata tttaagtaaa tacgtgtaaa
    20401 atgaatataa agttttgaaa tataaagttt ttgtatctaa gatattttat ttgtaaaata
    20461 gtaattaatt attgtaagct ataatatgag agtattatgg ttaatgtgaa ttttaaagat
    20521 attattaaat atataaatta gaatactatt aaacggataa atggaggagt tattatgtct
    20581 aagatattag tagtagatga tgagttagat atgttaaagt tgattgaaaa tgtgttgaaa
    20641 agagatggac atgaagtaaa ggtaattagt gatgtagaaa aagctatgaa catggatttt
    20701 aattatttta atctaataat attagatgtt atgatgccta agatggatgg ttttgaagta
    20761 tgtaaaacta taagaaataa ggttgattgt ccaatactgt ttttgacagc aaaaaatatg
    20821 gaaagtgata ttatgtacgg tcttggtata ggtgcagatg actatataac caaacctttt
    20881 ggtgtaggag aattaagggc taggataact gctcatttaa ggcgagaaag tagagaaaaa
    20941 aagaatttat taactatctc aaatgttcaa tttaactttt taggcaaaga agtttcaatt
    21001 gatggtaaaa aaattaattt tactaaaagt gaatatctaa tatgtgaatt tctagcaaaa
    21061 aataagggac aagtattttc aaaagaaaga atatatgagt cggtatatgg ttttgatgga
    21121 gaaagtgata taagtactat cacagagcat ataaaaaata tacgaagcaa attaaaacaa
    21181 tatgatattt atacaataga aactgtatgg gggatagggt ataaatgggt gtaataagta
    21241 taagtgttgt ttagtgaaaa tattatatat atctacaaaa tgtttgtaaa atattaatct
    21301 atatattaaa atttaattta tagattaata ttttttgtaa atgacaaaat tgtaaggtat
    21361 tttgttataa aatattaaga ttcatagtgt atactaataa tatagtattt agtataaatc
    21421 ttgagcaaag gatggttatt tctaatggat agactaaaga atattagaaa gagtttacta
    21481 cagtatacat ttttattttt gttaataggt ataacaattt acctagtgtt taaaacattg
    21541 gatattaaaa tgctatcaaa tgttatggtt atggtagata agaagttttt attcataggt
    21601 gctttggcaa taatgtgtca cataatgctt gaaggagtag taatgaagat aattatagaa
    21661 agcactcaca aagttaatat tagatttatt ggttttaagc tagccattat gggattttat
    21721 tataatttaa taactccttt tgcttcagga agccaaccag tacagatata tgtacttaaa
    21781 aaatgtaaaa tgccacttag taaggcaagt gcagttgtta ccaataagtc tattatcttc
    21841 caaatagtag ttacttttta ttgtacaata cttgttttaa tgaattttgc tatgttagaa
    21901 aaacaaatga aaatgattat gcctctagtg tttctaggga tagccattaa ttcatttgtc
    21961 ttaattatga taatattagt tattttaaat cctgaaaaaa taaaatattt tatgagaatt
    22021 actataaagc atttatcaaa aattaagttt ttaaaatttt tagataataa gattgaatcc
    22081 atagaaagtt ttatagatga ttatagtaaa gcaataaatt tttttgcaaa gaataaaaag
    22141 gtattaattt caactataat agtaactttt attcaattaa gtgcttactt tagtgtttca
    22201 ttttggatat ataaagcatt taatttacaa ggttatagct atatttatat actgacatta
    22261 caagcatttt tatatatggc aatatctcca ataccaacac ctgggaatgt aggagctaat
    22321 gaattagcct ttttcactat attcaaatca gtattccctc aacctttgat gggatatgca
    22381 gtatttttat atggtggatt tatgtactat ttgattctga ttggaagtgg aatatttact
    22441 gtaattactc actatagaat gaaaaacaga attggcagaa atattgtttt aagtgaaaac
    22501 taaataagtt taaagaatat atattgagta tattaaaaaa tatgtcataa gaactagaat
    22561 attttaatta aatggttgaa atataaagat tattatgaca tatttttttt gaaatttaag
    22621 aggaattgtg agaaaattgt tgaatttaat agatagtatt gcaaaatatt tatgattttg
    22681 catattataa aaacactcat tatctttaag ataaatttta cttatcaggg gataaatcta
    22741 aggggggaat tgtatggaga aaaaaatttt aacagtttgt ccatactgtg gagctggatg
    22801 ccaattatat ctagtagtaa aagataataa gatattaaga gctgaaccgg ctaatggtag
    22861 aacaaatcaa gaaagtttgt gtttaaaagg tcgttatgga tgggattttc taaatgaccc
    22921 taaaattcta acacctaggc taaaaaagcc aatgataaga aaacaaggtg tactagaaga
    22981 ggttgaatgg gatgaggcta taaactatac agcttcaaga ctaaatgaaa ttaaagaaaa
    23041 atatggacct gattctatta tgggaacagg ttctgcaaga ggacctggta atgaagcaaa
    23101 ctatataatg caaaaattta tgagagctac aataggaact aataatgttg accattgtgc
    23161 tcgtgtctga catgcaccat ctgtagccgg tctggcttac tcattaggta gtggtgcaat
    23221 gtctaattct ataccagaga tagaaaatgc agatgtttta tttatatttg gatataatgg
    23281 agcagattca catccaatag tggcaaatag aatagtaaaa gctaaaaaaa atggtgctaa
    23341 attaattgtt actgacccta gagtaactga atctgcaaga attgctgata tacatctacc
    23401 tataaaaggt ggaacaaaca tggttttagt aaatgctttt ggaaatgtat taatagaaga
    23461 aggtctttac aataaagaat ttgttcaaaa tcatacacaa ggatttgatg agtataaaga
    23521 aattgttaaa ccatatacag ctaaatatgc tgaaaagatt acaggaatcc cagaagagct
    23581 tataagaaaa gctatgagag aatatgcaaa aggtaaaaaa gctatgatac tttatggaat
    23641 gggtgtctgt caatttggtc aagctgttga tgtagttaag ggacttgcat caattgctct
    23701 acttacaggt aattttggta gagaaagcgt tggaatagga cctgtacgtg gacaaaataa
    23761 tgttcaagga gcttgtgata tgggagctct tcctaatgta tatcctggct atcaaaatgt
    23821 aactgatgat aagataagag aaaagtttga aaaagcttgg ggggtaaaat tatctcctaa
    23881 taatggttat agtttaactc aagttccaaa tttagtacta aaagagaaaa agcttaaagc
    23941 ttattatata tttggagaag acccagtgca aagtgaccca gatgcatcag aagttagaga
    24001 agcattagat gaattggaat ttgtcattgt tcaagatata tttatgaata agacagcact
    24061 tcatgctgat gttatacttc cagctacttc ttggggagaa catgagggag tatatacatg
    24121 tgcagataga ggattccaat tgatgagaaa agctatagag ccacaaggag atgtaaaacc
    24181 agactggcaa attataagcg aaatttctac agcaatggga tatcctatga attataaaaa
    24241 tactaaggaa atatgggacg aacttagaca attatgtcca agtttcttag gtgctactta
    24301 tgaaaagata gaaactcaag gatgcgttca atggccttgt aagagtgaaa gtatggaaga
    24361 taaaggaact atgtatctgt atgaaggtca aaagttttct actcctaatg gtaaaggtaa
    24421 tttgttcgct gctgaatgga gaccccctat ggaagttgaa gatgatgaat acccatttag
    24481 tttatgtact gtaagagaag ttggacacta ttcagtaaga acaatgactg gtaattgtag
    24541 aacacttagt tcattagaag atgaaccagg acgtgttcaa ataaattcta atgatgctga
    24601 aaaattagga attgaagatg atgaattagt tagaattagt tcaagaagag gaagtgtaat
    24661 aacaagagca acagttactg atagagttaa ggaaggtgca acatatatga cttatcagtg
    24721 gtgggttgga gcttgcaatg aacttacaat agcaaattta gaccctatat ctaaaacacc
    24781 agagtataaa tattgtgctg ttaaacttga aaaacttgaa gaccaagaat tagcagaaaa
    24841 atgtgtaaga gaagagtatc aaagtttgaa agacaaaatg acagctacaa atatctaatg
    24901 atttattaga tttaagattt ataaaaggca gaaaggctaa tattttatga acaatatata
    24961 ctcaaaaact aaattacaag aaaatagtaa ggataaagtt tttgaaaata gttatagtct
    25021 agtggactac aaatatttaa attcttttaa tgtagaagta agcaaaataa atgaacatga
    25081 agtatcaata gataatgaag aaataattgc agaatatcca cttagtttta ttttaaatga
    25141 taaatatgta aatacatttt tatgtacccc atataattta gaggaactta tagtaggatt
    25201 cttgaaaaca aaaggataca tagaaaacaa aaaaagctta ttaaaatttg agataaatga
    25261 gagtgaacgt tttgcaaaag taactattca agagaaacaa aaaaatcaag atgagcaaat
    25321 tatattttta aattcactag actatataac atgtgctcct gtaaagagca atataaaaat
    25381 aaattatgat acgatttaca gtataatgga taaaaattta aattcatcag aattatttaa
    25441 aaatactggt ggggtacata gcgtatctgt atttaatttt gataaaaaaa tagtcacttg
    25501 tgaagatgta gcaaggcata atgcaatgga taaggctatt ggattttgtg ttttaaatga
    25561 cataacatta aaagataaaa ttgtggctgt aagtggaaga atatcattgg aaatgatatt
    25621 aaaagcagct atgactcaaa ttcctattgt agtatcaaaa tcagcaccga ctaatttatc
    25681 aatagagctt tctaaaaaat taaatataac gcttattgga tttgtaagag gaagacggat
    25741 gaatatttat gcaaatccac agagagttat caaaaagtga gaatgaatat atgtgtacta
    25801 aaaaagaaga gcaaagatat tcaaagtgtg aaatctgtgg agtattaatt gatgatactg
    25861 ataaggtgta taataacaca tacaatttgt gcaaaaaatg taaacgaaat ataagtactg
    25921 agaaaatagt gaaatatata aatatatatg atgaaagtaa gtagacatat gatgaaaata
    25981 actagagggt tggaaatata caaattgaaa ggtgggataa tatgaaaaat aaaataggct
    26041 cttttgtaat agcagaccca aacaaatgta caggttgtag agcttgtgaa gtagcttgtt
    26101 ttacaatgca taatcagcat aataatgtgg gttatacagt tggaacagtt gatatacctg
    26161 ttatacctag actttactta gtaaaaggtg acaatttctg tatgccaata cagtgtagac
    26221 attgtgaaga tgctccttgt ttaaactcat gtccacaaaa agctatagtt aaagaaaaca
    26281 atattatgtc tgtaaatgaa gaaaagtgta taggatgtaa aacttgtctg ctagcatgtc
    26341 cttttggagc tatagactta cttccacaat atgaagatgg taaagaagta gaacaagtaa
    26401 ttttagatga aaataagaag atagcttata aatgtgactt atgtaaagac aatgaaaaaa
    26461 tagcttgtat aagtgcatgt cctcaggaag cattaaaact tgttactcca atagatgaca
    26521 aaaaagctaa gaatagacaa gctgcactta gtttattgtt aacaaataaa taaaggatac
    26581 aggggtaggt gacgtttatg ataataaata tagataagga tttatgtact ggatgtagag
    26641 aatgttcaaa ggtctgtcca gtaaatgcta ttgaaggtga agagggaaag ccacaagaga
    26701 taaatttaga tagatgtgtt atgtgcggtc aatgtgttca aacttgtaaa tcttatgcct
    26761 cagttataga tgaaggattt gagtttttac aagagaaaaa acaagaaaga gagataccag
    26821 agtctataaa tgaacctata tttgcagcat acaatgtatg tgatatagat aaggtgaaga
    26881 aagctttaag tgacccaaat gtattcacta tggtccaatg tgcaccagca gttagagttg
    26941 ctttaggaga agattttgga tattatttag gatacttagg tgctgggaaa atggcagcag
    27001 cacttagagc tttaaatttt gatagagtct acgatactaa ttttgcagca gatttaacta
    27061 taatggaaga aggtagtgaa ttaattaaaa gagtgacaga aggtggaact cttccaatgt
    27121 ttacatcttg ttgtccagct tgggtaaaat tcatggaaaa aaattatcct aaattaacta
    27181 atcatttatc ttcttgtaaa tcacctcagc agatgggtgg tgcaatattt aaaacttatg
    27241 gtgcagagat aaataatgta gatgcatcaa agatatttag tgtttctata atgccatgta
    27301 catgtaaaaa atatgaatgt gatagagaag aaatggattc tagtggatat agagatgtag
    27361 atgtagtatt aactactaga gaactagctt atttaataaa agacatggga attgatttta
    27421 aaaatttaag agaagaaaaa tttgatagtc ctttaggtag ttatagtgga gctggaacta
    27481 tctttggtgt tactggtggg gttatggaag ctgctataag aacaggatat gagcttataa
    27541 ctggagaaag tattcctgat gtagaagtaa aacaagtaag aggagaaaat ggttttagaa
    27601 agtcaactat taaaatagga gacttagact taagagttgg agtagtaagt ggattaaaaa
    27661 atgtaatacc agttcttgaa gacttggaaa aaggaaaatt agatgtagat tttattgaag
    27721 ttatgacttg tcctgttggg tgtgtaagtg gtggaggtca accaaaaatt ttactagaag
    27781 aatataagga attggcttat gaaaatagaa caaaagcaac atacgtgcat gatgaacatt
    27841 tagcaattag aaaatctcat gagaatcctg atattaaaaa actgtatgat gaatatctgg
    27901 tagaaccatt aggagaaaaa tctcatcatc tattacacac tacatattgt ataggaaagg
    27961 aagtggctaa gtgatgaact attttgttat agcagaccct gataaatgca taggatgtag
    28021 aacttgtatg ataggatgtg tagttgcaca ttcagatgaa gatatttttt accaaaatcc
    28081 agatgaaata aattttaatc caaaactttc tgtcataaag acaaaagaag taagtgctcc
    28141 aatacagtgt agacattgtg aagatgctcc ttgtgcaaag gcatgtccaa atggtggtat
    28201 agttagggtt ggtaacacaa taaaaataaa tgaagaaaat tgtataggat gtaagacttg
    28261 tatgttagca tgtcctatag gtgcaataga catagttaca ttaaaagatg tagatgaagg
    28321 aaagctttgc tttagagaaa gaatgacagc aaataagtgt gatttttgta tagaatcgcc
    28381 agaagggcct gcatgtgtaa atgtttgtcc aactaaggca tttactatag ttaaggatga
    28441 ggatatagat agtaaagtta aaaataagag aaaattagct atattgtaat ataaaaaaga
    28501 tggagtttta tttatgatta aaagctccat cttttttatt ataatattta tatatctaaa
    28561 aatcaattat tgcattagtt tatctaatct ttctttattc aattttttca ttcctataga
    28621 agttataata agtaaagtat aggcaattat aactgataaa attataatag accaagaaaa
    28681 tctgtatcca aaattatcaa ctaacaatcc aaataatgtt gaacctaatg caaatcccac
    28741 tgcaaaaatt aattgtacga ttccatatat gctaccaaac tccttttgac caaagaaaga
    28801 gccagttaaa taagcaggac caatcatata agcaaataca gaaagacctt tcaaaatggc
    28861 aaatacaaat gcaaaagtag aagatttatc taccaatagt aatgataaag ttgctataag
    28921 aaccaataca aaagatataa taagtggctt tgtttgaccg aatttatcaa acaataatcc
    28981 tccaaataaa ttaccaatga gagcaaataa ggcaaatgtg gagcctactg ttcctattag
    29041 aataggattt agataatttc taagataagt tggatattgt acagataatg atgaaacata
    29101 tataccaaca aatataaatc caatagtcat aatccaaaaa tatttaattt ttttagcttc
    29161 ttttaatgta taaccccata ctgaaggaga aattgtatca gatgctttct cttttttacc
    29221 tttagatttt accttttcat taatatcttt aggcatcctg agtatgaata atattacagg
    29281 tataccaact attaatgcta taattccaaa tattagataa gaacgactgt atccttgagt
    29341 tgctaataag cgaactgcaa gtggttgtag aaatatacta ccaatagaac caccagcaaa
    29401 agcaaagccc aaagcttttc ctttgctttc ttcatcaaac caagcactta ttaaaacagg
    29461 tattcctata gcagatataa ttgtagcgcc aatttgaaca atacatgata cagtgtaaaa
    29521 ttgccatagt tcagtacata tagaaaaagt agcaaaagca atactagaaa ttatacaacc
    29581 taataaatat agtatcttaa tattgacttt agaaaataat acacctataa aaggtgatgc
    29641 aattgctgag gcaatagtac caatagtgaa tattagagaa aaagaagcta aactaaagcc
    29701 ttctccttct acaacataac ttataaattg aggctgtata cttgctacaa tactaaatgg
    29761 aacagcttgt attaacatac aaccaagtac tataacccaa gcatttgaaa agtttttaat
    29821 gctaaattta ttgtttagta atgtattcat ggtatctctc ctttaggtta tgatttgtag
    29881 atattaatct aattattcta attttatata ttagttttgg atagcctaac tatttaaata
    29941 taatttgggt attaatataa aaattaacta taaaaattga ttattttaaa tgtatggggt
    30001 aaattaaagt atataaataa atgaaaggat ggataaaatg tcatttgaaa aaataaataa
    30061 taataaccta gataaaatac acaataggaa gtgtattatg cttgtaaact ttaataaaaa
    30121 agaattatca ttaattaaaa atgtaagtaa ctttgctgga ataagagacc atatcatctt
    30181 aagtgataaa aatggagaga atataataag agacatttta gatggaaata tatcaaatga
    30241 atgtgaaaat gggattaaga gtaagtctgt aatatttaat aacactccaa gtacacaagt
    30301 aaatgcattt ttagaagggc taaaaaaagt gagaataaat agacctttta cagctatggt
    30361 aacagagaca tctattgatt ggacattgaa taatttaata gaaaatttag ttcaagaacg
    30421 taatgctatg tctttaggaa aaacaattga acataagtaa atttaagata tcttatagta
    30481 attttaacta atgtacattg agtatcatat tatattttag aatagaaaat tttaaatgtg
    30541 ggtagatgga ggagatttga gatgaaagtt ttatttacaa taaattatgg aaaagaaaaa
    30601 tttgataaaa taagagaatt aggctatgat gtcatacatt actctgaaga tagtattgaa
    30661 aataatgagg atgtaaatac agcagatgta ctagttacat acaatccatt tgagagatta
    30721 gatatttcta agatgaaaaa tttacaatat atacagacaa ctagtgtagg tatagaccaa
    30781 cttccaaaag aagaggtatt gaagagaaat attaagatag ctaataatag aggcggatat
    30841 agtgtaccta ttggtgaaag tattgtattg tatattttag aaatatataa aaatagtgca
    30901 aaatttttta aacaacaaca aaataaagaa tggaagatgg atttttctgt atcagaattg
    30961 tatggacaaa gagttggatt tttaggaaca ggtactatat cttcagaagc agcaaaaaga
    31021 ttaaaagctt ttggtgtaga aatatggggt gtcaatactg atggaagtag taaaaaatac
    31081 tttgataagt gttttgctac tgaggatatg aatattgtat ttgaagaatg tgatatagta
    31141 gttgtagcaa tgccttctac aaaaagtaca gatggaatag taaacaaaga tatgtttaaa
    31201 cttatgaaag atggttctgt atttataaat gtaggtagag gaaatacagt taatcagaaa
    31261 gatttagaag aatgtgtaga gaaatttaga ggggtagcat tagacgtatt tgaaagtgaa
    31321 cctttaaaca aggacaataa gttgtgggag tgcgaaaatg tcatagtaac tcctcataat
    31381 tcttgggtgt cagataaaaa caaagagaga acatttaata tggtttataa taatttgaaa
    31441 cactatataa tggagaataa acctgtagat aatgttgtag acatatcaag aggctattaa
    31501 aaataaaaaa ttttgataaa aataaaaact cttattagtc gtattaaaga tactaataag
    31561 agtttttttt gtaataaatt taaagcccaa aaagtgatat actaaaaaat atataaatag
    31621 gagataatta attataggga aatttcaaaa taatttaatt ttaaaaatat gaagaggtgc
    31681 aaaaagtgag cgtaaaagat gaaattaata aacttataga gacaataaac taccataatg
    31741 aaaggtatta taatcaagat agcccagaaa tatcagatta tgaatacgat aagttaatga
    31801 aggagttaat ttctttagaa gaagaacatc cagaactaaa gagaattgat tctccaacaa
    31861 atagggtagg aggtaagcca ttagataagt tcaatcaaat tgtacataaa actcctatgt
    31921 tgagtttatc aaatgctttt tcagagcaag atttgagaga ttttgataag agagttcaag
    31981 aatatgtggg agaaaatgtt gaatatgttg ttgaatttaa aatagatggt ctttctgtag
    32041 gtctaactta taacaatgga gagtttgaaa aaggagcaac tagaggagat ggagttgtag
    32101 gagaagatat ttctcaaaac ttaatgacag taaaaagcat acctcttaag ataaatgata
    32161 aaaaagaact aatagttaga ggagaagtat atatatcaaa agataatttt agaaaagtaa
    32221 atgaacaaca agaagaacaa gaacagccat tatttgctaa tccaaggaat cttgcagctg
    32281 gttcattgag acaattagac ccaaaactaa cagcaaagag accattagat atatttgtat
    32341 ttaatatgga aaatatagaa gaatacgatt tagaaagtca cagtgaatca ctagagtact
    32401 tagaaaaact aggattttca gtaagtcaga gttataaagt ttgtaaaagt atagatgaag
    32461 ttattgagca tattgaatac tggacagata acaggggtaa tttagactac gaaatagatg
    32521 gtatggtaat aaaagtaaac aatataaaac aaagagacga aatgggatat acagccaaaa
    32581 gtcctagatg ggcaattgca tataaattcc cagcagaaaa gaagaaaact aaaatagtag
    32641 atataatagt agaagttggg agaactggta caataacacc ttcagcaatt cttgaaccag
    32701 tcagacttgc aggtaccact gtaagtagag ctacacttca caatgaagat tatataagag
    32761 aaaaagatat aaaaataaat gatacagtat tagttcaaaa agcaggggac ataatacctc
    32821 aagttttaga agttataaaa gaagaacgta ctggagatga aatagatttt aaaatgccag
    32881 aaaagtgtcc agtttgttca gagccaacaa taagattaga aggagaagct gctgtaaaat
    32941 gtatcaatat gtcttgccca gcccaaataa gaaggggaat tatacacttt gtgtcaaggg
    33001 atgcaatgaa tatagatgga ttgggagaat ctataattac actgctatta aatgaaaaaa
    33061 ttattcaaga tgtatctgat ttatattata taaaaaagga agatgtagtc gacctagaga
    33121 gaatgggcga aaaatctgct gacaatttaa taaattcaat tgaaaaatca aaagaaaatg
    33181 atttatggag acttataaat ggattaggta ttaaatttat aggagttaaa gcagctaaga
    33241 tacttgcata taactttaag gatttagatg aagtaatttc tgcaactagt gagcaactag
    33301 aagaattaga ggaatttgga agtataatgg caaacagcgt tgtcgagttt tttaaggaag
    33361 acaaaaatat gaacgttata aacaagttaa aaaatgtagg agtaaataca aaatcattgg
    33421 ataatacagg tgaaaaagta gaaaaaatat ttgaaggtat gaaaatcgta ttgactggta
    33481 cacttcctac attaaaaaga aatgatgcaa aagatatgat agaaaaaaga ggtggaaaag
    33541 ctacatctag tgttagtaaa tcgacaacat ttgttttggc aggtgaagaa gctggttcta
    33601 aacttactaa agcaaatgat ttaggaatta aagttataga tgaagagaga tttttagaat
    33661 tattaaagtt atcatcgaaa gatgaagtaa aagctgtatt agagagttaa aaagtcgctt
    33721 aatttagtag aaaactacta taattaatga tagaatagtt cagtaagcta ataagattac
    33781 gaaaaatgac aataattaat atttgacaaa attaaaaatt ataaaaataa atttataaaa
    33841 aataaaaaat atgagaaagg atataaaaaa tgaccagaag ttatgataga agtaatgaac
    33901 agataagacc tgtaaagata actagagatt atacaaagta tgcagaaggt tctgttctta
    33961 tagaaatggg agagacaaag gttatatgca ctgcatcagt ggaagaaaaa gttcctcctt
    34021 ttttaagaaa tagtggtact ggatggataa atgctgaata ttctatgcta ccaagagcaa
    34081 cacaacaaag aaagataaga gatgcatcta ggggaaaaat tgatggaaga acacaagaaa
    34141 tacagaggtt aataggaaga acaataaggt ctgttgtaga cttgaataaa ttaggagaaa
    34201 gaactatttg ggtagattgt gatgtgattc aggcagatgg tggaactaga actgcatcta
    34261 taacaggggc atttgtagca gttgcagaag caatatataa attatataaa gcaaagacaa
    34321 taaaagagat gccaataaca aattttgtgt ctgcaattag tgttggtatt gtaaatggaa
    34381 tacacatgct agatttatgt tatgaagaag attctaaagc acaggtagat atgaatataa
    34441 taatgactga taagtgtgag tttgtagaag tacaggggac aggagaagag aaaccttttt
    34501 ctagagcaga tttaaattta ttgttagagt taggtgaaaa agggaataaa gaattaataa
    34561 ggcttcaaag agaagcttta ggtgaaatat cagatgagat attgggtatg gattatggta
    34621 atgaagtagt gattgctact aataatgctc ataagctaga agaaataggt gaaatcttaa
    34681 aagattttga atataaagtg tactctttaa aagatgttga tttagcaggt atagagatag
    34741 tagaagatgg aaaaacattt gagcataatg cgttgataaa agctagaaca atagcaaaaa
    34801 agactaaact tatagcaata tcagatgatt ctggcttaga agtagatgct ttaggcaaaa
    34861 aacctggtgt ttattcagct agatatgcag gagaacatgc aacagatgaa gaaaatagaa
    34921 aaaaactgtt aaaagctatg caaaatgtac caatgagcaa aagaaatgca agattcgtct
    34981 ctgcaatagc agtagttttt cctgatggaa aagaatttgt agtaagagga atttgtgaag
    35041 gaatgatagg atttgaagaa aaaggtaaaa atggttttgg atatgatagc ttatttatag
    35101 taaaaggata tgataagact tttggtgaaa tacctagtgt tataaaaaat tcaatcagtc
    35161 atagagcaaa cgcactaaaa cttatgaaac aagagtttat aaaaagggta gtgaagtaat
    35221 gaaaataggc atagtaagcg acacacatat gatgattaaa aatatggaaa aaacaatacc
    35281 atatttgaaa gaatgtgacc taataattca tgcaggagat aactttactg attcaagata
    35341 tattcatagc atgactaatg taggtattat tgcagttaaa ggtaattgtg actttgatgc
    35401 tgttgaggaa gaagttgtct ttgaagtggc aaataaaacc atattcttat gccatggaga
    35461 taagtatggt gtaaaatatg gaactaatat gttagaaaaa aaggcaactg aagttgatgc
    35521 tgatatagtt atatttggcc atacccatac cccatttaga gaaattaaag atggtgtact
    35581 ttacataaat cctggaagca cttcacttcc aagaggagta tcgtataaaa gttttgtcat
    35641 tatggatata gaagaagatg acataaaaat tgaagaaata cgcatataaa ggtgtattta
    35701 tctaaaaaaa tggcaaaaaa cgctcaaaaa attaaatttt atcaaataag tacaacgata
    35761 ttaatggaac acttgtaaat aaaaaaactt atgtgatata attaagtggt tgaattataa
    35821 atagaatgaa ttataatacc gttatcgggt attacataaa tatcactagg aggattagaa
    35881 ggatgaaagc agaactaatt aaaaaagagg gtaataaagt tactttaaaa ataacagtag
    35941 ataacaataa atttgaagca gcagtaaata aggcttataa taaaagcaga aataaataca
    36001 atatacctgg atttagaaaa ggtaaagctc caagaattgt tatagagact caatacggaa
    36061 aaggtatatt ctacaatgat gctatagaaa tattattccc agaagtttat ccagaagcta
    36121 tcaaggaatt agacatagac ccaatagaca atccagattt agatatagaa gaaataagta
    36181 aagacaatgg gttggttatg gttcttaatg tagaagttaa accagagttt gaattaggaa
    36241 actacaaggg tatagaaata gctaaggttg agaatactgt aagtgacgaa aatgttgatg
    36301 ctaaactaca agaaatggtt gaaaaaaatg ccagattagt aagtgtagaa gacaaagctt
    36361 tagaagatgg agatacagct ataatagact tcgaaggttt tgaaaatggt gtagcttttg
    36421 atggtggtaa aggtgaaaat tataacttag ttataggttc aaacacattt ataccaggat
    36481 ttgaagaaca attagtaggt aaaaaagctg gtgaagaagt agaagttaat gttactttcc
    36541 cagaagaata tcactcacaa gatttagctg gaaaaccagt tgtatttaat gtaaaaataa
    36601 atgatgtaaa agtaaaagaa ttatctgcac ttgatgatga atttgctaaa gatactagtg
    36661 aatttgattc tttagatgaa ttaaaagcag atgtaagagc taagttagaa gaagaagcta
    36721 aaaatagagc agatgcagaa actagaaatt cagtagtaga aaaagttgct gaaaatacag
    36781 aaatagaaat accagaagtt atgatacaac atcaaataga taatatgtta aatgaactta
    36841 actaccaatt acaatatcaa ggatttggat tacaacaatt actagagatg actggtagaa
    36901 ctatggaaga attaagagaa gagagaaaag aagatgctaa aaaattagtt aagtcttctt
    36961 tagtattaga agcaataact aaagctgaag gaattgaagc aacagaagaa gaatttaagg
    37021 ctgagttaga aaaaatggct tctgcttaca atatggaagt agaaaaaata gaagcttctt
    37081 taagagatgc tgacaaagaa gatataaaag gtcaaataaa aataagaaaa acaatagatt
    37141 tattagttga taatgcaaca atagcttaat aaaataaacg ctaactaata tagttgacgt
    37201 tatgaaatgt ttagtatata atgatgtgca aatataattt agttaaatgc gtataataat
    37261 ttacagtagc ttaaaaaaag ctactgtaaa ttatatagaa taaagggggt atgaaaccat
    37321 ggcattagta cctgtagttg ttgaacaaac aggaagagga gaaagatctt atgatatttt
    37381 ttctagacta ttaaaagaca gaataatctt cttaggagat caagttaacg atgccacagc
    37441 agggcttata gtagcacagc tattattttt agaagctgaa gacccagaca aagatataca
    37501 tttatatata aattctccag gaggaagtat aacttctgga atggctatat atgatactat
    37561 gcaatacatc aaaccagatg taagtactat atgtataggt atggctgctt ctatgggagc
    37621 attcttgttg gcagcaggag caaaaggaaa aagactagca ctaccaaata gtgaaataat
    37681 gatacatcaa ccattaggtg gagctcaagg tcaagcaaca gatatagaaa ttcatgcaaa
    37741 gagaatttta aaaataaaag aaactttaaa tgagatatta tctgaaagaa ctggtcagcc
    37801 attagaaaaa attaaaatgg atactgaacg tgataacttc atgagtgcac tagaagccaa
    37861 agaatatggt ttaatagatg aagtatttac aaagagaccg tagatagaga gaggtgttaa
    37921 tatgtctaaa tatgaagaaa aacgacaatt aaagtgttca ttctgcggta aaaatcaaga
    37981 tcaagtaaga agattaattg caggtccaaa tgtatatatt tgtgacgagt gtgttgaact
    38041 ttgtgacgaa attattcagg aagaaataga agatactata gatgaagata ctacatcttt
    38101 accaaaacca aaggaaatga tggagattct aaatgattat gtaataggtc aagaaaaagc
    38161 taaaaaagct ttatcagttg cggtttataa tcattataag agaatataca gtaaaaaatc
    38221 tagtagtaag gatatagaaa ttcaaaagag taatatactt ttattaggac ctacaggttc
    38281 tggaaagaca ttacttgctc aaactctagc aagaactcta aatgtacctt ttgcaatggc
    38341 agatgctaca tcattgactg aagctggata tgttggagaa gacgttgaaa atatactttt
    38401 aaaacttatt caagctgctg attttgatat agaaaaagct gaaagaggta taatatatat
    38461 agatgagata gataagattg caagaaaatc tgaaaaccca tctataacta gagatgttag
    38521 tggtgaaggt gttcaacaag cacttctaaa aatattagaa ggtactgttg caaatgttcc
    38581 accacaagga ggaagaaaac atcctcatca agaattttta aagatagata ctactaatgt
    38641 attatttata ttaggtggag cttttgatgg attagagaag ataattcaaa aacgtggtgg
    38701 agataaaaca ctaggttttg gagctaaaat agaaagtaaa aaagaattag atttagggaa
    38761 gctgtatgaa aaagtacttc cagaggattt attaaaatat ggtataatac cagagtttat
    38821 aggaagaata ccagttttag ctactttaga gttgttggat gaagatgcat taatgcagat
    38881 attacaggaa cctaaaaatg ctctagtaaa acagtataaa aagttattgg agttagatga
    38941 tgttgagcta gaatttgaag aaggtgctct tcgtgcaatc gctaaaaagg ccatagaaag
    39001 aaatacagga gctagaggac ttagaagtat agttgaaagc gtaatgatgg aaactatgtt
    39061 tgaagtacca tctagagata atataaagaa ggttatagta acagagaaat ctgtgaacga
    39121 agactcagta aatcctatca tagtacttaa ggatcaagag gaaagtgcat aagatgatga
    39181 aaaggagctt taaaagctcc tttttttatg ttaatatgct aaatataaaa taggtatcat
    39241 tattataaaa atgtgagtaa tatacctaga ataccaaaga cgaatacaat tgcaatggaa
    39301 ccaatttttc ctttatataa aagataaaat gatgctataa atgcaattat tccatataaa
    39361 tcccaattaa cttctttggc agatgaaaaa gctgctgata atatgaatcc tagagtaatt
    39421 ggtctaagaa ctgtcaataa gttttctacc tttttattat ttttaaattt agaaaataac
    39481 tttgatataa tagttagacc tattaatgca aaaattgtaa caccaatagt tgcagctaga
    39541 gaaccaaaga agccagcagt cttaaatcct acaaatgtag cactatttat tgctacagga
    39601 cctggtgtag attgtgatat agcaagtaaa tccaaaaact cttgattagt tataaagtga
    39661 tatttagata taaattcttg ctggataaat ggtagcatgg cataaccgcc accaaaacta
    39721 aatgccccta ttttaaggaa tattaagaag agtagtatta tttctgacat agactatata
    39781 cacctcctcc aattattaaa taaatagggc ttatattgaa tacaccaaca gataaaactg
    39841 ctataattaa tagtagtatg ttagttttag actttggtag tttcttgaac atgctcgtaa
    39901 aagaatacaa tataagagct attacaacag gcgcaactcc tttaaaaaat ccatctaaaa
    39961 ttttagatga tgaattttta aagtatagat acgataagac taagactata caaaatgatg
    40021 gtaaagcaga gcctaaggta catataagaa ctcctggtat accataaagt ttatatccta
    40081 ataatacaga tatattaact gccagtactc caggataact ttgagctatg gataaataat
    40141 caataaattc ttcctgctct aaaagttttt gtttgtttac aagttcatct tgtataagag
    40201 gaaccatagc gtatccacca ccaaatgtaa acgcaccaat ttttaaaaag gttaaaaaca
    40261 atttcaacat aatagaaacc tccaaaagat aaataacatc taagtatact aatattaata
    40321 ttatatcata ataaaaagat aatatagtat gatagtattc taagaattta tcattaatgt
    40381 tgaaaattaa cttgttcaaa tatataatat taaaagcaaa gcaattagac tagaaaagga
    40441 gtaatgttga tggaacaaaa ttatactaaa atagatcatg aattaccatt aattcctcta
    40501 agaggattag ctatatttcc atatatgata ttaaattttg atataggtag agagatatct
    40561 cttaaagctt tagaccaagc tatgatggat gaagaactta tatttttaac ttctcagaaa
    40621 gaagcagagg tagatgagcc aggagaagag gatttttatc atgtgggaac aatctgtaaa
    40681 gttaagcaaa tgataaaatt accaggagat acagttcgtg ttttggttga aggtgtatca
    40741 aggggtagag taaagaaaat agaacaagaa gatgggtatt tcagagcagt aatagaagaa
    40801 atagtatttg atagtgataa tttggatagt gaaacagaag ttgaaattga agcatttgta
    40861 agaaatgtat ttgatgcctt tgaagagtat ataaatattg gaaatagggt atcaccagaa
    40921 atattaatat ctttagctga tatagaagat gtagatagat ttatagatac gatagctgca
    40981 aacatatatc taaaatcaag ccaaaaacaa gaaatattag aagaatttga tattagaaaa
    41041 agacttgaac ttatttattc tatattatta gaggaaatag atatattaaa aatagaaaag
    41101 aaaataactt taagagtaaa aaaacagatg aataaggttc aaaaagaata ttatcttaga
    41161 gaacaattaa aagctataca gaaagaatta ggtgaagaag aagatataaa ctctgaagca
    41221 gatgagtata gagaaaagct taaaaaaata aaagcaccta agacaactaa agagaaaata
    41281 gaaaaagaga tagataaatt ttcaaaaata tcttctatgt ctccagatgt atctgtaagt
    41341 agaaactatt tagatactat attctcattg ccatggaata aagagacaaa agataaatta
    41401 gatataacta aggcaaaaga tatattagat gaagaccatt atggattaga aaaagttaag
    41461 gagagaatac tagagtatct agctataaga actcttgcta agtcacttaa agggcctata
    41521 atatgtctag ttggtcctcc aggaacagga aagacatcta tagtaaaatc aatcgccaga
    41581 gcattaaata gaaaatttgt aagaatatct ttgggtggag ttagagatga agcagaaata
    41641 agaggtcata gaagaactta tgtaggttca attccaggaa gaataattaa tggggtaaaa
    41701 gaagcccaaa caaaaaatcc tgtattctta tttgatgaga tagataagat ggcagcagat
    41761 tataaaggtg acccagcttc agcaatgcta gaagtattag acccagagca aaataaggat
    41821 tttgtggacc attacttaga aatacctttt gatttgtcaa aaatactatt tgttacaaca
    41881 gcaaatagtc taagtaatat accaagacca ttattagata gaatggaagt aatagaagta
    41941 tctggatata tagaagaaga aaaattaaat atagcaaaaa aatacctact tccaaaacaa
    42001 attaaagaac atgctttaaa agaaaacttt ataaaaatag atgatgaaac tttaagaagt
    42061 ataatcaacc attatacaag agaagctggt gtaagaacac tagaaagaac tattggtaag
    42121 atttgtagaa aggttgccaa aaaatacgtt gaagacccaa ctcttgaaga ggttgttata
    42181 aataagtctg acctagagac atatcttgga aaagacatgt ttaagtatca acttgcagaa
    42241 gttaaccctc aaataggatt agtaaatggt cttgcatgga cagaagttgg tggagttaca
    42301 cttgaagtag aagtaaacgt acttaaaggt aaaggagaaa ttgtattaac tggaaaatta
    42361 ggagatgtta tgaaggaatc tgcgaagaca ggtatctctt atataaggtc aatagtggac
    42421 aagtttgata tagaccctga attttataag acgaatgata ttcatataca cattccagaa
    42481 ggtgctgttc caaaagatgg accatcagct ggtataacta tggctcttgc agtaatctca
    42541 gcacttacta aaagacctgt gcctggaaat atagcaatga caggagagat aactttaaga
    42601 ggtagagtct tagcagtagg aggagttaaa gaaaagttat tagcagctca tagagctggt
    42661 ataacaaaag tattaattcc taaagaatgt gaagctgatt tagatgaaat accagaaaat
    42721 gtaaaagaaa aaatggagtt tgtgcttgta gaacatatgg atgaagtatt agaacaagct
    42781 ttattaaaga gtggtgaaaa taatgaaaat tagaagttca gagataacta tgagtgctgt
    42841 gaataaatca cagtatccag cagaaggaat cccagagata gcattggctg gtaggtcaaa
    42901 tgttggaaaa tcatcaataa taaatacatt gcttaacaga agaaacttcg ctagaaccag
    42961 tcaaacacca ggaaaaacta gaactataaa tttttatcta ataaataatg aattttattt
    43021 tgtagacctt ccaggctatg gatatgcaaa gatagctaag tctgaaaaag aaaagtgggg
    43081 aggtataatg gaacgatatc tagaaagtag acaagagtta tgttctatat tcctacttgt
    43141 agatattaga catgagccta cagcagatga taagcttatg tatgaatgga taaagcactt
    43201 tggatataac tgtgtagtta ttgcaactaa ggcagataaa atatctagag gacaatatca
    43261 aaaacacata agtataataa gaaaaaaatt gcaaatggaa agtagtgaaa aagtaattcc
    43321 tgtttcttca ctaaaaaaga ctggtgttga agaattatgg gaagagattg taaatcaata
    43381 caatcaacat ggatatgaaa taacagtaga ttaaatttaa aatgttaaat attgacatga
    43441 gatttatttg aataataaat gtaataatag ttagttttaa ttatattaat attaaagcaa
    43501 aatataggat gccttaaaat agttttaatg aacttttttt ggcatcctta ttgtttataa
    43561 acaataaata tcagtaattt tgttaaatac taataaattt tagtaaggta tgggaaaatt
    43621 agtataaatt agttcaacta aaaaattatt gacttatgta gtcagcaata aatagcattt
    43681 aagaaataga tttatctgtt tttctcataa agaaaacagt tagtataagc cctataaaac
    43741 atactgatgc tgcaatccaa taagactgat tgctagctat aacataggct gatgtgtgac
    43801 ttttaaactt ttgattgtat gtaagacttt gtgcaaaaaa actactaaaa aatgctgttc
    43861 caagagcaaa tgataaatta cgaatagttg caagaaaact gcctccgtat ccctgttttt
    43921 gaggaggaac atctgctaaa atactactat tgtttggtga actaaatgct cctagcccaa
    43981 gtccaaaaat aactaaacat acaattaaaa gatacaatgg agaatctgct tttaaaagtg
    44041 ccattccaac tagtgagatg gtcataaatg aaaaagatgc tgttagaatg ttttttgcac
    44101 cgtgtttatc agaaagtgaa cctgcaatag gtgctataag taccataata agtggatgta
    44161 cagtcattac ataaccagcc atcataggag ataaattttt taattgttct aaataaaatg
    44221 gaagtagaaa agatactgcc atttgaggaa aataacaagc tactccaatg atattgccta
    44281 cagtaaagtt tctattttta aatagaggta ggtcaataaa gctttgttca aattttactt
    44341 ctcttaatgc caacaataag aagattataa gtgctgttat acctaaatac aatcttgtat
    44401 tacccttctc attcattgca agtaatataa gcgtagtgca agatgcgaaa agtagaagac
    44461 ctggaatatc aaagctcttg ttttgttcat ccaaaacagg tttaggtaga aatttaatgc
    44521 caagaaagac tgctataata ccaataggta tattgataat aaaaatagaa ggccatccaa
    44581 aatgttctaa aattatacct ccaattacag gaccactcat atttcctata ccaactacag
    44641 ctccagtgat tcctatagct tttccttttt catttgctgg aaatgcattg gaaactatac
    44701 caagcccagt agccattaaa atacttgctc caactgcctg aaagattctt gaagaaatta
    44761 atgttgataa attgtttgac atactacaca gaaaagaacc tattgtaaat ataaaaaatc
    44821 ctaatgtgta tagacgattt gaacctactt tatcgccaag ttttccgaaa aataacatag
    44881 tagctgttac aacaagcata taagctgtta caacccactg aatttgtgac atgttaattt
    44941 taaagtcagc agcaattgta ggatttgcga tatttagtat acttccatca agagtagaca
    45001 taaatacacc aatacagatg ataatcaata ctatccattt ttgtttcatg ataacactcc
    45061 aatcttaata gattttataa gtacgatata attgtaaaat aaacaacaaa aaaagcacct
    45121 gtatattagg taccttgaat gtgtctaatt tcatgaataa atacggtagt tgaaggattt
    45181 gtatagatta ctcatgtaaa attacataaa ggtactcata tactattttc ttctaccatc
    45241 cagattttac tgtcggcttt ggaatatgac caaattatgc cataaggctc gcgggctttt
    45301 accgccgata gggaatttca cccgtttatg aagaaaaaca gatgtatttt gtaatattat
    45361 tttaacataa aattataatg gaaacaattg gatttaagta atatccagct gtattacaat
    45421 acaaatattt caatatggac acttaaattg tatttataga tagaataagg aacagtatga
    45481 gaatctataa taattagata aaattaacct gttattagta ttagatggta agttttaaat
    45541 ttatcaaaat cagaaagctg taataaaatt cttataatat gaatatctta attaagagat
    45601 attgtaaatt atgaggaggc gctaatatgg aaggaaaggt aaagaaggca ttcccgggtg
    45661 gaaatacatc acaaggctca tattcttttt ttgactatat aattcctgaa aatgtaaaaa
    45721 gagttttttg tttaaagggt ggacctggag taggaaaatc ctcacttatg aaaaaaattt
    45781 ataaagagtt tttgcataga ggatatgata ttgatttata tcattgtcct tctgacccaa
    45841 gttcgttgga tggtttagtt ataaagaaat taggcgtggt acttatggat gcaactgccc
    45901 cacatactat ggacccaaag ctacctggag ctttggatga gataattaat ttaggtgatt
    45961 actggaatac agaagaatta gaaaagaata aacaagaaat atcagtttat gataaagata
    46021 ttagtaattc atttaaaaga gcatttaagt ttttaaaatc agcagaacca atctttagag
    46081 acatagaaga aaaatattct gattgtatgg attatggaaa agtaaatttg gtcacagaag
    46141 aatttataaa aaggttattt gatggagtta aaagcacagg aaatctaaaa agagaaaagc
    46201 atttatttgg ttcagctata actcctgtag ggtgtttaga ttatacagaa aacattttaa
    46261 aagatgtaaa gaaagtctat tatttagatg gagaaatagg aactgggaaa actacattat
    46321 taaacaaagt atataagaaa gctattgaaa aaggtttaaa tgtagaagtt tatcattatc
    46381 cacttattcc agagaaaatt gaaactgtac tattaactga tttagatata ggcataacta
    46441 taagtacgac ttttaaagaa gaagatacaa ttgatttaaa tcagttttta aatagagaaa
    46501 aactacttaa atatgaagaa gatattaaat ttgatgaaaa agttttagat gatttaattt
    46561 gttgggcagt actaaatctt aagagagcca agtctaaaca tgatattata gaaagttact
    46621 atagtcctaa tatgagattt gatgaagtag ggaaattaag agataagtta attaaaagaa
    46681 ttttaaaata tgaggaaaac ttataaacgc atatgtttaa aattaacgtt agtaataata
    46741 taatttttag taaattggtg tagctttgag gctatgccat tttacttttt ttgctaaaaa
    46801 atgatattaa taatctccaa agccattatt tgtttttcat aattattatt tcctttccta
    46861 atttatgttt ttttataata tcacaacaat gtttattgaa agtttatata attttaagaa
    46921 catctaaaaa tgcatacttg atattggtaa attagtgata ggagttcaat gtatgatatt
    46981 atatactaaa gatgaatttt aatatataac tttgatataa taagcatatg taaatgttta
    47041 atatttaatt aaagaaaatt atcatataaa aataaatttg taaggagcaa tacagtatga
    47101 aaaaacaata tgtgtttata atactagcaa gtactatttt aggtatactt gttggaacca
    47161 tatttagaga ttatagcaat gaagaaacag tttatacaag caaagacaag tcaataagaa
    47221 aagaaataaa aatgacaaga aaatctataa aagaattaac caaagagaaa gacaagttag
    47281 ataaagaaat taattcaatg aaacaaaatc aaaaaaattc tgaagaaata tcagaaatag
    47341 aaacacttag agatagtcta tcatattcag atgtaaaggg tagtgggata atattaagta
    47401 tagatgctat agatgaaaaa acagggaata tagctaatag tatagactat aataagattc
    47461 ttgtaaaagt tataaatgaa ttaaaagtaa acggaggaaa atacatagaa ataaatggtc
    47521 aaagaattaa tcaatattca gaagttgtac ttgcaggaag ccatataaat ataaatggag
    47581 ttcctgtagc acaaccatat gaaataaagt gcattggaga taaggagaaa ctatctaatt
    47641 atgtagataa aggtagtaaa tatttagata gcatacaaac caattatcca atgaaaataa
    47701 gcataaaaac tgaagaaaat atctctatga aaaaaatgaa tatacctaat aaattaaagt
    47761 atataaaggg tgattaagat tatattagag gaaagcagtt tgccaatgtt aataccagaa
    47821 aaacttgcaa aaaaatataa tgtatttcca gttgagtttc aagatgataa tttagttgta
    47881 gaaatagaag atgaggatat ttatgctcta caggacttga gattagctac aggtaaagaa
    47941 attatattga agaaggagaa aaaagaactt ataagtgaaa agattgaaaa atattataat
    48001 agctttgaac ttgatgagga ttatgcaaaa aaattatttg aaaatctttt agaaaaagct
    48061 ataaaagaaa acgcaagtga tatacatata gaaccattta aagaacattt aatagtgaga
    48121 atgagggtag atggagaact aaaggaagta tcaaagtttt tagttgatgt ctatccatca
    48181 ttgtctacgg ttataaaatt aaaggcatca atggatataa cagaaaaaag gcttcctcag
    48241 gatggtaggg tggatataaa gttaggtgga aatttaattg acattagagt ttcatcaata
    48301 ccaactgtct atggtgaaaa aatagttttg aggatactta atagagatac ttttttaaaa
    48361 gacaaattag aacttggttt ttcagaggaa gctataaaat ctataaatcg tataataaat
    48421 aaaagagcag gaatattatt agtgacaggt cctacaggta gtggaaagac tacaacagta
    48481 tattccttat taaatgattt aaaaggaata aataaaaata ttatgactat agaaaatcct
    48541 gtagagtata agatggatgg cataagtcag attcaagtaa attcaaaagt agggttgagc
    48601 tttgatgtag gtcttagagc gatattaaga caagacccag atataataat ggtcggagaa
    48661 ataagagatg cagaaacagc taagatagca gtaagagcag ctataacagg tcatttagtt
    48721 ataagtactt tacatactaa tgatgcagta tcttccatag ctagattact agaaatgcaa
    48781 ataccaccat atcttttaaa tgcctctctt ataggtgtta tatctcaaaa gttggttaga
    48841 aaagtctgca atcattgtag ccatgagatt atgataaaag ataattttag tggagatgta
    48901 aatactaagg tagctgtagg gtgtgaaaaa tgtaatgata aaggatactt tggaagaaca
    48961 gcaatttatg aaatcttaga aattaatgat gatataaaga catgtataag aaatatggag
    49021 gattctagta ctattaaaga tgtagcacaa aataatggaa tgataacatt tgaagatagt
    49081 tgtaaaagac taataaatga aaaaataacg acattagaag agtgtttagt agtaaatgaa
    49141 atgatttaac tgaattgtat agttaaactt cgtttggtca gttgtcttta tttaatctat
    49201 tttaattaag atgtttgagt ataaattata tttaagtgaa ataaaattaa atgcattaaa
    49261 ttagaaaata ttaagtttat ttaattgtaa ttaatataat ttggaaggta ttatagtaca
    49321 tgagattatc taaaaatata ttttataatt tagaaaagtt ttatttagta ttaaatagaa
    49381 tcagtgataa agaaattaga gtaatttgca aagagatagg catattgctg gaatctggat
    49441 gtgaaattac aaaaatattt gaaatcattg agagtcagtc aagtaaaaaa gtaaagaact
    49501 tgcttagtat agtatcaaat catattcaaa aaggtaactc aatagcagaa tcatttcaaa
    49561 taacaggtat attttctaaa ttctttataa gtatgataaa agcaggagaa actagtggaa
    49621 acttagatat aataatgagt gatttatcta attattatga taaagaatac aaattaaaaa
    49681 tgaaaataat aactatttca atatatccaa taatacttat aatattatct atcttatcaa
    49741 tgttgtttat attcgtattt gtaataccta attttcaggt ggtttttacc aacaatggaa
    49801 ttgaaccacc tttaataact agagttttaa tgggaatatc tacagtggtt acaaataact
    49861 tagcttatat aatatttagt tttatattgt tttcaatagg aagcatttat ttctttataa
    49921 caaatgataa tataaaaaaa ttgattaatt cgttaaaatt caaaatacct tttataaaaa
    49981 aaattaatca gttagtagct acaacaagat tttcaagggc attgtccgtt ttaattagca
    50041 gtggaataca aattgtggaa gctatagata tgtcagctag tgttatagac aacgaattta
    50101 tttatgaaag gttaaagatt tctaataatt atataaaaaa aggaaatagc ataggtaaat
    50161 ctttgaattt agcaaatatt tttccaaaat tatttatttc aatggcaaat acaggagaag
    50221 aaagtgggaa actagataaa agtctaaatg taataaataa attttatgaa aatgaattaa
    50281 ataacaaaat agagcaaata atgaaattta tagaacctat gctaattgta tttatgggat
    50341 taattattgg tacttttatt ttagcaatgg ttgttcctat gtttgatgca attactaatt
    50401 tttagagaga aaaataaatt ttattagatg aaacttttga aaaggagaga atatttaatt
    50461 gataaatttg ataaataaaa aacgaaaagg atttacactt gttgaaatga ttgtagtagt
    50521 aactatttta ggcgttatat ctagtatagc attagttaag tatagtaagg ttcaagaaag
    50581 tgccaaatta aatgcagact atacgaatgc tgctaatata gtaaccgcag ctagcatggc
    50641 aattaatgat gatgaaaaga caataaactc tctaagtgta gagacattga aggaaaaggg
    50701 atacctaaat actgttccag ttcctcagag tacatcaggt aaattcgaac ttgtcataaa
    50761 tgatagcgga acagatataa gcgtaaatat aaattctaag caattttatc caaaataata
    50821 ttttatattg attgctgtaa gttttaaaat tatttttatt taagaaggag atggctataa
    50881 ttaaaaaaat tctaaactta gaaataaatg atgattatat aaggcttgta tttttaagaa
    50941 aaagtcataa aaaagttttt atagataaat caattataaa agagattaac tttgatataa
    51001 aaaatgatag taattcgatt gagaagatta aatatataga agatgtagtt agatttgtaa
    51061 aagaggagat tggtaaatca aaaatattat ataaaaattt atactttaac attcaaattc
    51121 aggatatagt cattagaaat gttgaagttc taaatactaa gaaaaaaaga gacatattaa
    51181 gcatgattga atttgaaata acacaataca ttccaataaa cataaataac tattcaataa
    51241 aatataaagt tataaattct aaaaatgata aattgaatat acaagtaatt cttttcccaa
    51301 agaatattat tcaattaatt aagaacattt cagaaaatct aaatatgaat ccaaagacta
    51361 taaatataaa ttttgatata ttacaaaagc ttataaactt aaatttaata gagaattttt
    51421 cagaagaagg ggtatttata gaatgtaaaa aaagtcagtt aattataaat ataacaaaag
    51481 ataaaaaaat aagagaaaca tacttgctat caaaagaggg aaaatcatat gagttcataa
    51541 aaagattatt aaggggtttt aagtatgttt attactatgg tatagaaaat tgttttattg
    51601 aagaatactt taaatataca gataaatcta ttgaagtaaa tccattaaga ttaaaagata
    51661 aagtaaaagt agaaaataat tttgatgcag atttagaaga caataggata aattatataa
    51721 atagtatagg aatgattatt taagtgaaag tggataaaac aataaatttt cttaatggat
    51781 ttaaaaaaac taaaccatgt aataaaatct ataaaataat aatagtctta attttaacgt
    51841 gtattttatt taaatcattt atgtatgtga aatctataaa tgaacttgaa gattatataa
    51901 gtcaaaataa gctagtggat aaatcaaaga acactttacc aacaacagat aaaactagag
    51961 agaaattttc taaaataaaa cttaatgaaa taaaaaaaat atatgaaaca attggaataa
    52021 atagtattac taaaatatca atagataaaa ataagctaga gatggaaggt gtgtgtagtg
    52081 atttagatgt tttaaacaat ttaaggaaaa taaagtacat aaaatattat tacatagaca
    52141 gtattgtaag agatggagaa tattatagat ttagaataaa aagttagatg ggtgattatt
    52201 tttgagatta aataatagag aaaaaaatct acttaaaata ttgttaatat tagttattat
    52261 attggcaagt gatatagcag taagttattt ggaagaaaac ctaactagtt tgaagaatga
    52321 aaagcaaaat ataataaata aaataaataa tcaagataat tatcaaataa aggataataa
    52381 gcatatagat gataaatata tagaaagtgt tgaattaaat aaaaatgaag aattgaaaaa
    52441 taattttaat aactcatttt tagataaaaa aacaaataaa gatatagtgt tattagtaga
    52501 aaatgaactg agagatattg tgaatgtaga atatattaat caagaaagta gtttagataa
    52561 tcacaataaa aaacaagtta atttagaaat aaaagttaat ggagaacttg caaatgtgtt
    52621 gaaacttgaa ggtgtaatga agaagttaag actaaataat aaacttgaaa agatagaaat
    52681 taacaaatta aaagtcaata attttgatgt tcaaaatatt ccaaatgaat ataaacaaga
    52741 aaaagacaat attgttgaat gtaaaatgac agttagagtc atataacaaa aaagaataga
    52801 taaagtaaaa aactattatc aattaataaa attttataga ggaattagtt ttacgataat
    52861 tcatttctac aagagggatt gttgatgatt ttatcgaaat cctaattaaa gttaatatat
    52921 tgtaccaaaa tataagtgaa tttaatagaa tatttactta tactatagat tgattaataa
    52981 aaaaatggta taataacaaa ttgtacttta atttaataat aagggagtgg aagtcatatt
    53041 gttagggaga aatgagctat gcccttgtgg aagtggcaaa aaatacaaga aatgttgttt
    53101 aaataaagat attgtttcag aaagagcatg cagaaaaact gaactttcac aaaaacaata
    53161 tacagattta tattcaaagt tatatgatta ttcaagacag gataagttta aagaagaatg
    53221 tgaaaaagct aaagaaatgt tttatataat gcaaaatgat agtgtaaatg aaaaatttga
    53281 aagatttttt aatacttatt taatacaaga ccacataatg gaaaataaaa aggttatgac
    53341 agttggtttt cttgaagaaa atggtgcaaa cctaagtcaa tcagaagtat caatattaaa
    53401 aagtatgttt gaatcatacg taagtattta tgaggtaaaa gaagtttctg ctggaaaaat
    53461 agtattaaag gactgtttaa atggaaatga actttgtaca gaagatgtaa aacttctgag
    53521 aagttttaaa atcggaagtt gtatgatagc tagaatagtt gatattgaag gaactaacat
    53581 attgatagat ataactataa gtatatcaaa tgaggttaaa gatataattt taaaggatat
    53641 tatgaatcta ttcaatcaat atcaagatgt atacaaagat atgaagtcgt tcttaattta
    53701 tcatacacat atactataca agtatataca acagctttta gacccaagta tagctgaata
    53761 tttaaagaga gaaagagata aaaacgatac aaaagttaat tcagaaaatg aattagctga
    53821 agaagaatgt aaagttttag atactcttaa agaaaatgta gaaaaagaaa atatagatgc
    53881 atgtatagag ttttggaatg aatataagaa aaatcatgaa aatataaaag gagctgaaaa
    53941 tggttgggca gctgctgttg aatactatgt taaaaaagaa gcaggacaac ctataactca
    54001 agttcaaata tcaaagaaat acgatataag ctctagtaca ctaggaaaga gatacaagga
    54061 tttaaaaata tcttaattaa ttataaaaag ctttaagtca tagataaaga atctatagta
    54121 taatatatgt ataatgttta gaaagaggta ttaataatga gtcttagttt aggaaatgca
    54181 attgctgtat caacgaactt tataaaatct aacccagctg tacctgtgta caacggagaa
    54241 gaaatatatg cagttattta tcatatagaa ataggatata ataatggtgt atcacctgat
    54301 accaaacaat atataataat agactataaa gagtttgatt tatctatgga taaaaaaaat
    54361 gaactaataa atattgctaa agagtactgt aaatctaaag gtattgtaga aaacgaaaac
    54421 cttgaaataa accttttaga caatgaagaa gcagagaact tcttagaaaa ggtgtttaat
    54481 aatatgaagg ttataagagg tcttataact aaaaaaggat aaataaatgg actatatagg
    54541 aatagaaaat ataacacctt atgaaaatac atatgaattt agtgtatatg aatatgatga
    54601 tgaaatcacc ttaggtagtg aaaagttata tgtatgtgaa ttaagggttg tattgattaa
    54661 agttaattct ctgtatgttg aaagattgca taaatcagtt gaagcaatgg tcttagtaaa
    54721 aaacttgaaa aaagatttag ataaaacact tgttgtaaac aaaataaaga attttgtgct
    54781 agatgagatt tgggtagaaa atctagtaaa agagaatata gaagttatat ttgtagaaag
    54841 ctaggaataa ccctagcttt tttatattac aatctaagag aggaaaacaa tatgttaaga
    54901 gtatctaatg taaaacttgg aattgataaa gatatatctt tattaaaaga tgttatactt
    54961 aagaagctaa aaataaaaga aaaaaactta gtcaaatata gtatatataa agaatctata
    55021 gatgctagaa aaaaaggaaa aatggaattt gtttattctg tagatgtaga agttaaagat
    55081 gaaaataaaa tacttaactc aaaaataaaa gatgtaacaa aaataaagga aatcagatac
    55141 gtaaatgtac ctgtgggtga taaaaaatta aagaataatc ctttggtaat tggtagtgga
    55201 ccagctggct tgtttgcagg tctgctttta gctcaaatgg gttatgaacc aatcatactt
    55261 gaaagaggtc ttgatgtaga cagtagaacc aaagatataa gtgatttttg gacaactaga
    55321 aaattaaaaa ataattcaaa cgttcaattt ggagaaggtg gagcaggaac tttttcggat
    55381 ggtaaactta caactagaat aaaagatata cgatgtagaa aagtattaga agagttagtt
    55441 cagtttggag caccagatga aatattatac tcacataagc cacatgttgg aacagatata
    55501 ttaaaaaatg tagttaaaaa tataagaaat gaaataataa gtttaggtgg tcaagttaga
    55561 tttaattcaa aagtaacaga tatagttgta tcaaatgatt ctatacaatc tgtaataata
    55621 aatgatactg aaaaaataga tacagataca attatattag ctgttggaca tagtgcaaga
    55681 gatacttatg aaatgttgta ttcaagaggt ataaaaatag ttcaaaaacc ttttgcaata
    55741 ggagctagag ttgaacatcc acaagattta ataaataaag cacagtacaa ggatttttat
    55801 aatcatccta ggttaggagc tgcagattat agacttatag agcatgtttc aaatggaaga
    55861 acagcatata ctttttgtat gtgtccagga ggtagtgtca tagcatctgc atcaaatgat
    55921 tttgaaatag ttacaaatgg tatgagtgaa catgcaagag ataaaataaa tgcaaatagt
    55981 gcatttttag taaatgtaac gccagaagat tttaagagtg ataatccact agcaggtgtt
    56041 catttccagc agaagtatga aaaattagct tttgaattag gtggaaaaaa ttataatgca
    56101 ccagttcagc ttgttggaga ttttataaac aataagactt ctttgaattt aggaagtgtt
    56161 gagccatcat atagaccagg atatacactt acaaatttaa gtgaatgttt gcctagcttc
    56221 gtaacagaga ctatgagaga agcgtttata tctcttgata aaaaattgaa tgggtttgca
    56281 atgtatgatg ctgttttgac gggagtagaa actaggtcat catcaccaat aagaatagta
    56341 agagatgaaa atactttaga atcagtgagt attaagaatc tatatccatg tggtgaaggt
    56401 gctggatatg caggtggtat agttacagca gcagttgatg gtataaaatg tgcagaaaaa
    56461 ataatacaaa aatacaccaa taatacaaaa atttaacata aacttaatga ttttttaata
    56521 tttaacatga atttaacatt atcactccta taatttgata aaatacaata ggttataaaa
    56581 tttttagata cagtaatctc tttacaagga ggaaataatt tgaacattat aataagtcta
    56641 atgggtggat taggtctgtt cttatatggt atgaacctta tgggagaagg tcttcaaaaa
    56701 tctgctggga caaagctaaa aaagataata aaactactta ctagtaacct atttatggga
    56761 gttttggtag gtacaggagt tacagcagta atacaaagtt caagtgctac tactgtaatg
    56821 gttgttggtt tcgtaaatgc aggaataatg actttaaaac aggcaatagg agttataatg
    56881 ggtgctaata tagggactac tgttacagcc caattggtat ctttcgactt aactggtatg
    56941 gccccagtag cattaggtgt aggtattata ctttatttat ttggaaataa gcctagaata
    57001 aaaaatatag ccgaaatact tataggtttt ggtatattat ttacaggtat ggattttatg
    57061 aagatggcag tggagccact aagagactat caaggattta ctgacttact tgttacattt
    57121 ggtagatatc cactacttgg attattattg ggatttggga ttacagcaat aatacaaagt
    57181 tcaagtgcat ctatgggtat gttggttgca ctggcagcag aaggtctagt gccattatca
    57241 gcagcactgc caattcttta tggccaaaat attggtacat gtgtaacttc actgttatca
    57301 agtattggtg ctaacaaaaa tgcacgaaga gcagctatga tgcatctaat cttcaatgtt
    57361 ttaggaacgg tgatattctt aatattcttg aataaaccag tagtaagcat ggttacaagt
    57421 tgggacccaa gcaatgttgc aagacaaata gccaatacac atacattgtt taatataata
    57481 tctgtattga tacttttacc atttacaaat cttattataa aactagctat aaagcttgtt
    57541 cctgatagag caggtgatat agatgaagat gaaactaaaa ctattaaata tatagatgat
    57601 agaatgatag aaactccttc tatagctctt gcaaatacga taaaagaagc acttcgtatg
    57661 ggtgagaagg cgaaggaaag cctaaatgca tctatggaag ctttagtaga acattcaact
    57721 gaaaaaatag ataagacata taggcgagag agattaatca atgatttaca gaaagctatt
    57781 ttaaattatt tacttaaact ttcaaaagca cctttagatg atgattcaag agaagttgtg
    57841 gatactttgt ttaatacagt caatgatata gaaagaattg gtgaccacgc taaaaatata
    57901 gctgaattat cgcaagttgc aatagattca aatatttctt tctcagaaga aggacaatct
    57961 gaacttgatg ttatgtataa tagagttgtt tcagcatata cttatgcatt agaatcaatg
    58021 agaactgata atgttgattt agcttgtaag gttataaaaa ttgaagaaca agttgatata
    58081 atggagaaat cttgtagagc taatcacatg tatagattga acaataatct atgtagtata
    58141 gaaaatggag ttatttattt agacgtaata agtaatttag agagaatatc tgaccatgct
    58201 gtcaatatag cacaacaagt tatagcaaag agattaggaa atgattaaaa ataattaatt
    58261 taaaataata taaaatttaa aatagaggat aaataaaaga gaaactttaa atgatacgtt
    58321 tagagtttct ttttttgtaa atttatttga ctaaaatttt taaatttata catagttata
    58381 gcatttagaa gaatcgattt cctgataata aatgatgaga tatgtatgat tttgtggcaa
    58441 acttttaaaa aaaacaccta tattttgtgt tataatctct ttaaaacgaa tatgctgaaa
    58501 agagggattt aaatgggaaa aataaatttt gattatagta aagctacaga gtttttttgt
    58561 caaaacgaaa tagatgtcat gcaaccatat gtagatgttg cgcacgacat gttacataat
    58621 aaaactggtt taggaaatac ttttttagga tggatagacc ttcctaaaaa ttatgataaa
    58681 gaagagtttg acagaataaa aaaatctgct gataagataa aatcagactc agatgttctt
    58741 ttggtaatag gtataggagg ctcttattta ggttcgagag ctgctataga tatggtaagt
    58801 cattctttta ggaatggact taaaaaagag caaagaaagg ctccagaagt atattttgta
    58861 gggcataata taagctcaac atatattatg gatttattag atataattga aggaaaggat
    58921 atttctgtaa atgtaatatc taaatcagga actactacag aaccagctct ggcttttaga
    58981 atatttaaag attacttaga gaaaaagtat ggtaaggaag aagcaagaaa gagaatttat
    59041 gcaacaacag atgcaagtaa aggtgcactt agacagttag ctacagaaga aggatatgag
    59101 acttttgtaa taccagatga tgtaggtggt cgtttttctg tattgacagc agttgggtta
    59161 cttcctattg ctgctgctgg acttgatatt gatgctatga tgaaaggcgc aaatgatgct
    59221 agagaagcat ttcaaaatcc tgacttaaaa agtaatgata gttatagata tgctgttgct
    59281 agaacaattt tacatagaaa aggaaaagat gtagagttat tagtgaacta tgaacctcaa
    59341 ctacattatg taagtgagtg gtggaaacag ctttatggag aaagtgaagg aaaagaaaat
    59401 aaaggtttat tccctgcttc tgtagatttc tcaacagatt tacattctat gggtcaatac
    59461 atacaagatg gaaaaaggct tttatttgag acagtactta atgttgaaaa ctgtaagaga
    59521 aatataacta taagttcaga ggaagtagac ttagatggtt taaactattt agcaggtaag
    59581 actgtagatt ttgtaaatca taaagctttt gaaggaactc tattggctca tacagatgga
    59641 aaagtaccta atttagtaat aaatatacct caactagatg agtataattt tggatattta
    59701 gtttatttct ttgagaaggc ttgtggaata agtggatatt tattgggagt taatccattt
    59761 gaccaaccag gagtagaagc ttacaagaaa aatatgtttg ctttactagg caaaccaggt
    59821 tatgaaaaag aaaaggaaga gctagaaaaa agacttaaat aattttaaat agttaaggtt
    59881 taattaagat taaaataata taatgaaatc atcgaaaagg agtttactca aaatagatga
    59941 tttcattttt tttatgaaaa attgggaaaa atatattttg ataatttata ttatattaag
    60001 atagggagtg gtatgagatg tcaagaagaa agaaagggat aagtttagta atattagttg
    60061 ctataataag ttcaatactt agtagttttc taacaataat tttagttaag gacaatttag
    60121 taagtaaatc tacaggaagt tctacgccga tagttgtaaa tgatgatggt aagagtcaaa
    60181 atatttatca agcagttgca gaaaaagcta cgccatctgt tgtaggtatt acaacaacta
    60241 gtgttgatac tagtaatatg tttgcaatac caactgaaac acaaggtgta ggaactggaa
    60301 tcatagttga ttcaaatgga tatatactta ctaattctca tgttatatca gatggtcaag
    60361 ctacaagtgt aaatgtatta ttcaatgatg gttcaacaac ttcaggtaaa gtagtatggt
    60421 ttgaccaaca acttgattta gctatagtta aggttgataa aactggacta acaccagcag
    60481 aatttgctga tagtgacaaa gtaaaagttg gagatatatc aatagcaata ggtaatcctc
    60541 ttggattaga ttttcaaaaa acagttactc aagggataat aagtggatta gatagaacta
    60601 tacaaacaga aaaaactaat atgactggtt tattacaaac tgatgcaagt ataaatgcag
    60661 gaaatagtgg aggaccattg ttaaatcaaa aaggtcaagt aattggtata aacacagcaa
    60721 aagcttctca agcagaagga ttaggatttg caataccaat caatacagct aagagtatag
    60781 ttgaagaagt aatcaaaaat ggaaaatatg aaaaagtaac tttaggcata aaaggtacag
    60841 atgtatcaaa ttatgaagca gcaacaggta caaaattaag tactgataaa ggtgtttatg
    60901 ttgcagaagt aatatctggt tcatcagcag aaaaagctgg agtaaaggta ggggatatta
    60961 taactaaagt aggagataca gatataacag gtatgaatga tttaaataaa aagttgtata
    61021 ctttctcaaa aggtgctagt acaaaaataa cagtaaatag aggcggaaaa gcagtaacaa
    61081 taaatgtgaa tttctaaata tgaccagaaa gatagagact acatggtaaa gtgtagtctc
    61141 tatttttatt taatttttat agtatataca tctaaattaa ttaaaatgat gtattgttta
    61201 tagtttattg ctatattttc ttataaaaat aaatttaata aatatttaaa tttaatttac
    61261 aaaaacaata taaatatcat aataaaataa aattttttaa tattactaaa aatatataga
    61321 caaaataata acgaatatag tattatgtaa ttgtagaaat gcttttcata ggtatattct
    61381 atgaagatga attgcataaa aatgaaaaaa tggaatggat attatttagg ggtgaaaaat
    61441 catgaatcca ttagttataa acttacaaaa atgtagcata catgatggac cagggataag
    61501 aagtacagta ttttttaaag ggtgtccact tgaatgtgtt tggtgtcata atccagagag
    61561 tcaaacatat acaaaacaag ttttgtataa tgaagaaaga tgttcaaaat gtgaagcatg
    61621 tataaatata tgccctcata aggcaattta caaaggtgaa acaaaaatat gcttagacca
    61681 agataagtgt gagttttgtg aaacttgctt agactattgt gtaaacaatg caagagagat
    61741 tgttggtcaa gagtattctg taagagattt ggttaaagag atagagaagg atagaatttt
    61801 ctatgaagaa tcaggtggtg gagtaactct atcaggtgga gaagtaatgg ctcaagatat
    61861 ggacttcatt tgtggagtaa taaacatgtg caaatcaaag ggaatccatg tagcaattga
    61921 tacatgtggc tatgcaaagt cagaaaatta tgaaagagtt gctaaatgtg cagatttgtt
    61981 tttatatgat attaagctta ttgatgaaga taagcacata aaatttacag gaaaatcaaa
    62041 tgacttaata ctgaaaaatg ttaagatttt aagtgaatta ggggctaaca taaatataag
    62101 aataccatta attgtaggag taaatgtaga tgatgaaaat ttagaggtta aaaaaatgat
    62161 agagttttta aagcctttga atatacaagc agtaagtctt cttccatatc ataatattgg
    62221 taaacacaag tatgataaaa tttataaaaa gtatgaagga gaagaactac aaagaccatc
    62281 agaagaaaaa ttagaggaaa taaaaagact gtttgaagca agtaatttca atactaagat
    62341 tggagggtaa taaaatggca agaggaactt ttgagagaac taaaaaatta agagaagaaa
    62401 gtattaatgc agaaccacac atatctattg aaagagcagt acttatgaca gaagcttata
    62461 aaaaatatga gggaagtgta gagattcctg tacttcgtgc tttatcattt aagcattata
    62521 ttgagaatag aactcttagc ataaatgatg gagaattaat agtaggtgaa aaaggtgatt
    62581 ctcctaatgg agcaccaaca tatccagaaa tttgttgtca tacaatggaa gatttagaag
    62641 taatgcacaa tagagatata ataaatttta gcgtatcaga agaagctaga aaaatacata
    62701 aagaagaaat aattcctttt tggaaaaaga gacaaacaag agataagata ataaatgcaa
    62761 tgacacctga gtggttagca gcatatgaag caggtatgtt tacagagttt atggaacaaa
    62821 gagctccagg acatacagtt tgtggagata caatatataa aaaaggattt ttagatttaa
    62881 agaaagacat agaagcaaga ttaaaagagc tagattttct taatgattta gatgcatata
    62941 ataaaaaagc agatttagaa gctatggcaa tagcttgtga tgcaatggtt atattaggta
    63001 aaagatatgc tgagaaagca agacaaatgg cagaagaaga aacagatgaa gctaagaaaa
    63061 aagatttatt actaattgct gaaacttgtg atgttgtacc agctcacaaa ccagaaactt
    63121 atcatcaagc tatacaaatg tattggtttg ttcatatagg ggttactact gaacttaata
    63181 tatgggatgc ttttactcca ggaagacttg accaacatct aaatcctttc tatgaaagag
    63241 atgtagaaaa tggaatacta gatagagata gagcacaaga attacttgaa tgtttatggg
    63301 ttaaatttaa caatcagcca gcaccaccaa aagttggtat aacattaaaa gaaagtagca
    63361 catatacaga ttttgcaaat ataaacactg gtggaataaa tccagatggt caagatggtg
    63421 ttaatgaagt tagttatata atacttgatg ttatggatga aatgaaattg atacaaccta
    63481 gttcaaatgt tcaaataagt aaaaagactc ctcaaaaatt cttaaagaga gcttgtgaaa
    63541 tatcaagaaa aggatggggt caaccagcat tttataatac tgaagcaata gttcaagagc
    63601 ttatggaagc tggaaaaact atagaagatg caagacttgg tggaaccagt ggttgtgttg
    63661 aaactggatg ttttggtaaa gaagcatatg ttctaactgg atatatgaac attccaaaaa
    63721 tacttgaatt aacattaaat aatggatatg acccaattag taaaaaacaa ataggtattg
    63781 aaactggaga ccctagaaat ttccaatctt atgaggaact atttgaagca tttaaaaagc
    63841 aattacatta tatgattgac ataaaaatag aaggaaatgc tgtgatagaa aatatatgtg
    63901 caaaacacat gccttgtcca cttatgtcta caatagttga tgattgtata gaaaaaggca
    63961 aggattacca aagaggtgga gcaagataca acactagata tatacaaggt gttggtatag
    64021 gaactataac agatagttta acagcaataa aatataatgt atttgataag aaaaaattcg
    64081 atatggatac attgcttaaa gcacttgatg ctaattttga aggatatgaa gctatactta
    64141 atttagtttc aaataagact cctaaatatg gaaatgatga tgattatgct gatgaaataa
    64201 tgcaagagat attcaatgca tattacaatg aggtaactgg tagaccaact gtttgtggtg
    64261 gagaatacag agtagatatg ttaccaacaa cttgtcatat atactttgga gaaattatgg
    64321 gagcaagtcc aaatggtaga ctttgtgcaa aaccagtttc agagggaatt tcacctgaaa
    64381 aaggtggaga tactaatgga cctacagcag ttataaaatc ttgtgctaag atggaccaca
    64441 taaaaactgg tggaacatta ttaaatcaaa gatttgcacc atcagtagtt caaggagaaa
    64501 aagggttaga taatatggct aatctagtaa gagcatactt taatatggat ggtcatcata
    64561 tacaatttaa tgtatttgat aaaaatgtac tgttagaggc tcaaaagaat cctcaggatt
    64621 ataaagactt aatagttaga gttgcaggat atagtgacca tttcaataat ttaagtagaa
    64681 cactacaaga tgagataata ggaagaactg agcaaacatt ctaggtttgc ttgtaaataa
    64741 aacttcttac tatataaaat cgtaactgat ataataaagt ataaatggtt tgattccatt
    64801 tttaaaattt tagattaatt atgagctaac tttctaaata ggaagttagc tcgttaagtt
    64861 taatgaaaaa atcgttaaat ttatatttag taaatcatac acatattagt aagtaataca
    64921 tacaaaagaa taatttatta tagttataat aaggttatat tttataaaaa taagataatt
    64981 ttaagcagtt aaaataaagt caatttagct actttaaaat aaaactaata gaaaaaagga
    65041 gatttgatat gaaaacttta ggatttattg gttcagggaa tatgggaagt gcaatgatag
    65101 gtggcatagt acagtcagga ttagtaaagg cagaagatat aactgtatca gatttaagcc
    65161 aagttgcctt agataaagta aaagaaaaat ttggagtaaa tactacaact gattcaaagg
    65221 tagctgttac aaattctgat gtggtaatag tagcacttaa acctaatata tgcgaaaagg
    65281 ctttaactcc acttaaagaa ttaatagatg caaataaaat aatagtatct atagcagcag
    65341 gtaaaactat agaaagtctg gaaagtttta taggttcaga taaaaaaata gtaagagtaa
    65401 tgcctaatac tccagccctt gttggcgaag gaatgtcagc tctttgtaaa aatagtaatg
    65461 taacagatga agagttaaat atgattaagg ctctgtttga atcttttggt gaggcagaaa
    65521 tagtatcaga atatttaatg gatacagtta caggtattag tggttcttct cctgcatatg
    65581 tatttatgtt tatagaagct atggcagatg cagcagtatt agctggaatg ccaagacaac
    65641 aagcttataa atttgcatct caagcagtaa tggggtcagc taagatggtt cttgaaactg
    65701 gaaaacatcc aggtgaatta aaagatatgg tttgttctcc agcaggaact actatagaag
    65761 ctgttaaggt attagaagaa gaaggcttta gagcagcagt tataaaatct attgttgctt
    65821 gtatagataa gtctaaagat atgagcaaat aacagatgta attaagttta ctatatggtt
    65881 tcttgaattg tttttttagt gcttatttat atctatacat aaatgtattt ggtataagta
    65941 aaataagagt aatttactat aaaaaggaat tta
//
LOCUS       DAELTA010000005        56882 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.005, whole genome shotgun sequence.
ACCESSION   DAELTA010000005 DAELTA010000000
VERSION     DAELTA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.005"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..768
                     /locus_tag="KPG13_000255"
     CDS             163..768
                     /locus_tag="KPG13_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984003.1"
                     /translation="MIEKLNENASLSDLITTFENVKDELQIGKNNIASTLGNPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            792..1097
                     /locus_tag="KPG13_000256"
     CDS             792..1097
                     /locus_tag="KPG13_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984004.1"
                     /translation="MTEYVEVGRRIFFDEEGKIIFYEGQSKGNVPDRKNIKKIEYIDL
                     EYDYVDYDKYKIIGIDIRTKQPILEEIPVYMSEEGKRIQELENQILIAENEKSGGLL"
     gene            1097..1279
                     /locus_tag="KPG13_000257"
     CDS             1097..1279
                     /locus_tag="KPG13_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984005.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            1313..1879
                     /locus_tag="KPG13_000258"
     CDS             1313..1879
                     /locus_tag="KPG13_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984006.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            1910..2200
                     /locus_tag="KPG13_000259"
     CDS             1910..2200
                     /locus_tag="KPG13_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984007.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            2204..2461
                     /locus_tag="KPG13_000260"
     CDS             2204..2461
                     /locus_tag="KPG13_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG0984008.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            2722..3585
                     /locus_tag="KPG13_000261"
     CDS             2722..3585
                     /locus_tag="KPG13_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBG0984009.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            3664..4476
                     /locus_tag="KPG13_000262"
     CDS             3664..4476
                     /locus_tag="KPG13_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0984010.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            4633..6981
                     /locus_tag="KPG13_000263"
     CDS             4633..6981
                     /locus_tag="KPG13_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0984011.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDNNKEVNNTLPSVT
                     KIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVV
                     LVGNGLDNSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
     gene            complement(7023..7208)
                     /locus_tag="KPG13_000264"
     CDS             complement(7023..7208)
                     /locus_tag="KPG13_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBG0984012.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(7726..7902)
                     /locus_tag="KPG13_000265"
     CDS             complement(7726..7902)
                     /locus_tag="KPG13_000265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984013.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            8619..9296
                     /locus_tag="KPG13_000266"
     CDS             8619..9296
                     /locus_tag="KPG13_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0984014.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            9465..11501
                     /locus_tag="KPG13_000267"
     CDS             9465..11501
                     /locus_tag="KPG13_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG0984015.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(11692..12258)
                     /locus_tag="KPG13_000268"
     CDS             complement(11692..12258)
                     /locus_tag="KPG13_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0984016.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            12546..13481
                     /locus_tag="KPG13_000269"
     CDS             12546..13481
                     /locus_tag="KPG13_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0984017.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            13946..15160
                     /locus_tag="KPG13_000270"
     CDS             13946..15160
                     /locus_tag="KPG13_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984018.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(15580..16101)
                     /locus_tag="KPG13_000271"
     CDS             complement(15580..16101)
                     /locus_tag="KPG13_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0984019.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            16457..17254
                     /locus_tag="KPG13_000272"
     CDS             16457..17254
                     /locus_tag="KPG13_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0984020.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            17364..17954
                     /locus_tag="KPG13_000273"
     CDS             17364..17954
                     /locus_tag="KPG13_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG0984021.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            18020..19717
                     /locus_tag="KPG13_000274"
     CDS             18020..19717
                     /locus_tag="KPG13_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0984022.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            19931..20770
                     /locus_tag="KPG13_000275"
     CDS             19931..20770
                     /locus_tag="KPG13_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG0984023.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            21287..23143
                     /locus_tag="KPG13_000276"
     CDS             21287..23143
                     /locus_tag="KPG13_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0984024.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    23226..23466
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    23532..23767
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            23852..25069
                     /locus_tag="KPG13_000277"
     CDS             23852..25069
                     /locus_tag="KPG13_000277"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0984025.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            25186..25668
                     /gene="cdeM"
                     /locus_tag="KPG13_000278"
     CDS             25186..25668
                     /gene="cdeM"
                     /locus_tag="KPG13_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG0984026.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            25932..27197
                     /gene="hisD"
                     /locus_tag="KPG13_000279"
     CDS             25932..27197
                     /gene="hisD"
                     /locus_tag="KPG13_000279"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG0984027.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(27400..28152)
                     /locus_tag="KPG13_000280"
     CDS             complement(27400..28152)
                     /locus_tag="KPG13_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0984028.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(28270..28539)
                     /locus_tag="KPG13_000281"
     CDS             complement(28270..28539)
                     /locus_tag="KPG13_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984029.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            29014..30108
                     /locus_tag="KPG13_000282"
     CDS             29014..30108
                     /locus_tag="KPG13_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0984030.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            30110..31060
                     /locus_tag="KPG13_000283"
     CDS             30110..31060
                     /locus_tag="KPG13_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG0984031.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            31053..32237
                     /locus_tag="KPG13_000284"
     CDS             31053..32237
                     /locus_tag="KPG13_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984032.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            32250..33746
                     /locus_tag="KPG13_000285"
     CDS             32250..33746
                     /locus_tag="KPG13_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0984033.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            33769..34719
                     /locus_tag="KPG13_000286"
     CDS             33769..34719
                     /locus_tag="KPG13_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984034.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            34740..35702
                     /locus_tag="KPG13_000287"
     CDS             34740..35702
                     /locus_tag="KPG13_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0984035.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            36074..36706
                     /locus_tag="KPG13_000288"
     CDS             36074..36706
                     /locus_tag="KPG13_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0984036.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            37117..37209
                     /gene="kdpF"
                     /locus_tag="KPG13_000289"
     CDS             37117..37209
                     /gene="kdpF"
                     /locus_tag="KPG13_000289"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG0984037.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            37250..38932
                     /gene="kdpA"
                     /locus_tag="KPG13_000290"
     CDS             37250..38932
                     /gene="kdpA"
                     /locus_tag="KPG13_000290"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG0984038.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            38957..41014
                     /gene="kdpB"
                     /locus_tag="KPG13_000291"
     CDS             38957..41014
                     /gene="kdpB"
                     /locus_tag="KPG13_000291"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG0984039.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            41033..41659
                     /locus_tag="KPG13_000292"
     CDS             41033..41659
                     /locus_tag="KPG13_000292"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG0984040.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            41896..42804
                     /gene="cysK"
                     /locus_tag="KPG13_000293"
     CDS             41896..42804
                     /gene="cysK"
                     /locus_tag="KPG13_000293"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG0984041.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            42831..43421
                     /gene="cysE"
                     /locus_tag="KPG13_000294"
     CDS             42831..43421
                     /gene="cysE"
                     /locus_tag="KPG13_000294"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG0984042.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            43581..43769
                     /locus_tag="KPG13_000295"
     CDS             43581..43769
                     /locus_tag="KPG13_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0984043.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(43823..44692)
                     /locus_tag="KPG13_000296"
     CDS             complement(43823..44692)
                     /locus_tag="KPG13_000296"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG0984044.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(44889..46133)
                     /locus_tag="KPG13_000297"
     CDS             complement(44889..46133)
                     /locus_tag="KPG13_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984045.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            46424..46735
                     /locus_tag="KPG13_000298"
     CDS             46424..46735
                     /locus_tag="KPG13_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0984046.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      46828..46920
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            47020..47814
                     /gene="thiD"
                     /locus_tag="KPG13_000299"
     CDS             47020..47814
                     /gene="thiD"
                     /locus_tag="KPG13_000299"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG0984047.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            47838..48626
                     /gene="thiM"
                     /locus_tag="KPG13_000300"
     CDS             47838..48626
                     /gene="thiM"
                     /locus_tag="KPG13_000300"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG0984048.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            48649..49281
                     /gene="thiE"
                     /locus_tag="KPG13_000301"
     CDS             48649..49281
                     /gene="thiE"
                     /locus_tag="KPG13_000301"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0984049.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            49420..49722
                     /locus_tag="KPG13_000302"
     CDS             49420..49722
                     /locus_tag="KPG13_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984050.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(49788..50543)
                     /locus_tag="KPG13_000303"
     CDS             complement(49788..50543)
                     /locus_tag="KPG13_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984051.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(50531..51493)
                     /locus_tag="KPG13_000304"
     CDS             complement(50531..51493)
                     /locus_tag="KPG13_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984052.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(51497..51955)
                     /locus_tag="KPG13_000305"
     CDS             complement(51497..51955)
                     /locus_tag="KPG13_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0984053.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            52311..52667
                     /locus_tag="KPG13_000306"
     CDS             52311..52667
                     /locus_tag="KPG13_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984054.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            52739..53455
                     /locus_tag="KPG13_000307"
     CDS             52739..53455
                     /locus_tag="KPG13_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984055.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            53448..54272
                     /locus_tag="KPG13_000308"
     CDS             53448..54272
                     /locus_tag="KPG13_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984056.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            54284..55483
                     /locus_tag="KPG13_000309"
     CDS             54284..55483
                     /locus_tag="KPG13_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984057.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            55492..56304
                     /locus_tag="KPG13_000310"
     CDS             55492..56304
                     /locus_tag="KPG13_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984058.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            56315..>56882
                     /locus_tag="KPG13_000311"
     CDS             56315..>56882
                     /locus_tag="KPG13_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984059.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSK"
BASE COUNT        22388 a         6016 c         9643 g        18835 t
ORIGIN      
        1 tttaaaatag aaaagtgatt gattttaagt aattttttca aagtatatat agtataaata
       61 attttatttt taatagaaaa acaagcaaaa taaacaagta taaagctttt tagtaactct
      121 ttcttgacaa acagttacta aaaatagaaa ggtgatattt ttatgataga aaaattaaac
      181 gaaaatgcta gtctaagtga cttaataaca acttttgaga acgtgaaaga tgaattgcaa
      241 attggtaaaa ataatatagc tagtacatta ggaaatcctt ttgttggaac tgataagcta
      301 agtgtaacta aaaccaaaat acagaccttg aaaaatacac tagcaattag tttgtctatg
      361 aaaaatatat ctgctcaatc aactgaaaca cttcattcac taattggtaa agtagctgga
      421 atcgcaaata tttttattgc acaaggagaa tgtactcaag ttggagaaaa aacttttcaa
      481 ctctatcaag tagataataa tacgccaaaa acttgttcag caaacgcaat aagttttaac
      541 ttagctttta ctccaaaatt tattattgtc tatagaaatg cttacaatat aggtttgtgt
      601 agttttgttt tttactcatc ttttcgtaat attataacag cagtgcctca acctagcaat
      661 tttggtgaaa tgtacatgcc taacacattc gattgtgtag taggtcacac ttgtattttg
      721 ccagtgaaag attacaaaat accacatttt ttctatgcaa ttgcttaaaa aataagttaa
      781 aggaggaaac tatgactgaa tatgtagagg taggaagaag aatatttttt gatgaagaag
      841 gcaaaataat attctatgaa ggacaatcga aaggaaatgt tccagataga aaaaatataa
      901 aaaaaataga atatatagat ttggaatacg attatgttga ttatgataag tacaaaataa
      961 taggaataga tataagaact aagcaaccaa tcttagaaga aataccagta tatatgagtg
     1021 aagaaggaaa gagaatacaa gaattagaaa atcaaatatt gattgcagaa aatgaaaaat
     1081 caggaggact attataatga atataaataa tgttgtagta agaatattag cagagagaat
     1141 attaaacgga gggttaaacc cattgaaaaa tagagaattt cagttagatg acgtgactaa
     1201 cacagagtac agaaaggcta tagaggatta tattataaaa aatagtggag tagtagaagg
     1261 agcagaacca actatataga gggttctttt tttattgaaa gaaggtgact aaatgacttt
     1321 taaagagtta gttaataaag ttagaaatct tgtattagaa gcaaagaaag taaccataga
     1381 agatacagaa aataagttta caagtgaaaa tgtagaagaa gcattgaaag aatgtataaa
     1441 tagagcagat gaggcttttc aagaagccga tagtggaaaa gagttaattg caactgctat
     1501 cggctctcct gctacatcag aacaaacatt tcaagattat gcgaactata ttacaggatt
     1561 taagagcaat ataagcaatt tagaaactca attgaaaagc aaatattcta ttagatatgg
     1621 tcctattgat ggatatgatg gtaatccttt ttctgctaat tttggcaaga gtgcaagtta
     1681 ccttattgtc tatgtttact ttagaagaag tgtatattat tataatccca gtggtagttc
     1741 tttaggaagt aatacaggag gttctgaacg tgcatggatt actatagaca gcaataaaac
     1801 tggtttttca gttcattcat atgatactag ttatgagcca tatcctttta caggttatta
     1861 tattgcttgt ttcgcataat aaattatatt attaaaacta ggaggatgta tggaagaaat
     1921 tagcataaat ctattatgtg cagttgcagg agttgtaata tcctacttag catttagaaa
     1981 tagctcaaac agaaagatac aagatgatac agaaacaact acaaaattag aacaacaaat
     2041 aacttttctg tgtgagaatg taagagatat aaagcatgat gtagcaaagt ttaatacaag
     2101 tttcttagat atcagtgaac gagttgcaaa agtagaagca agtacaaaac aagcacatct
     2161 tagaattgat gaaattataa atagaattgg aggaaaataa aagatggaca atttaataag
     2221 ttttatacca gagcagttgc taattttagt agctgctctt tatgttttag gtgttggatt
     2281 caagaaatat aaacaattag ataataaata cattccagta gtgttactga tacttggaat
     2341 aggtttctca atatggatgc taggattaaa tcctgttgca gtcttacaag gtgtaatttg
     2401 ttggggagtt gcaataggta taaatcaaac ttacaaacag ttgaaggatg gtgaaaagta
     2461 atgaaattaa caaaaatatt attactcaca aaaatttaaa aaatttcatt aaatagagta
     2521 tacttttata gctaataagt atataataat agtacagaac tcgtaaagca tttatatgct
     2581 caaatatacg ggacattgat ttttgaagga acccgccaag cttcttttat agctcaaata
     2641 ggcgggacag ataaatttac ccactaatat taattggtgg gttttattat ataaaagtat
     2701 atctatgaaa aggtgaaaac aatggttgaa gtaaaagaag aaaaaacatt tgatgaacaa
     2761 atagatattt taaaaagtag aggattaata ataaatgata aagaagatgc taaatttgta
     2821 ttaagtaatg tcaattatta taggtttaca gcatatcttc taagttttaa gaatgatgat
     2881 ggctcatata aagaaggaac tacgtttgaa gaagtttatg atatatatag gtttaataag
     2941 gaatttagga tattattaac agatttgtta gggagcatag aaatagcatt taggacatac
     3001 attgcatata cattagcaat taaacatggt gcttgtggat atctagaaag ggagagtttc
     3061 aaagatgaaa aattctatat taattttttg acagcattag agagagaaaa aagtaataat
     3121 tcagataagc tttttattat acaccataaa gaaaaatatg aaggaaaact tcccatttgg
     3181 gttgcaacgg aaataatgac ttttggtatg ttgtcaaaat tatattcaaa catgttgcca
     3241 gaagatacta gatacataaa aaataatttg tgtagagtga atactttatt agttaaatct
     3301 tggttacaat cattaacaca ggtcagaaat caatgcgctc attatggtag aatatataat
     3361 aataatttcc gtattataac aataaaaaac gaatataaaa agtataactt ggataataaa
     3421 aagatatttt cttatatact tgctatgaag catttgacta tggataaatt aatttggaat
     3481 agttttttta taaaacttca aaagttaatt aatgattata ataattctat agacttaaag
     3541 cttattggtt ttcctaataa ttggatagag atattggcta aataaaatag ttactttaag
     3601 aagtttataa acacttacta tatgtaagtg tttttttatt gaaaagaagg aggaaaataa
     3661 ataatgaaaa tatgtattac agtaggacac agtattttaa aaagtggtgc atgcacttct
     3721 gctgatggag tagttaacga ataccaatac aataaatctc ttgcaccagt attagcagat
     3781 acatttagaa aagagggtca taaggcagat gtaataatat gccctgaaaa gcagtttaaa
     3841 actaaagcag aagaaaagac ttataaaata cctagagtta atagtggagg atatgattta
     3901 cttatagaac tacatctaaa tgcaagtgat ggtcaaggaa aaggttcaga agttctatat
     3961 tatagtaata aaggtttaga atatgcaact agaatatgta ataagctagg tacagtattt
     4021 agaaatagaa gagctaaatt agataaagga ttatatatct taaatagttc aaatcctaca
     4081 gcagtattaa ttgaaagttt cttctgtgat aataaagaag attatgagaa agctaagaaa
     4141 ctaggtcatg aaggtattgc taagttaatt gtagaaggtg tattaaataa aaatataaat
     4201 aatgagggag ttaaacagat gtacaaacat acaattgttt atgatggaga agttgacaaa
     4261 atctctgcaa ctgtagttgg ttggggttat aatgatggga aaatactgat atgtgatata
     4321 aaagattacg tgccaggtca gacgcaaaat ctttatgtca ttggtggtgc agcatgtgag
     4381 aagattggtt ctatgactaa agaaaaattt actatgataa agggtaatga tagatttgat
     4441 acactttata aggcattaga ttttattaat agataaatta aaaagtagca actaaaagta
     4501 gttgttatct tctaatgcct cattcataat aaaaaaataa tttattatat aataatatta
     4561 aagtttgttt ctaaggttaa tattatgtga tacaattcaa gtgggtaaat attatttgaa
     4621 tgaggtgtgg ggatgaaaaa tttaagcaaa aaagcagtta ttttatcact ttcattggta
     4681 gttgtatcac ctttggtaca taagataaat gcacaagata ttgagaaaaa tagggaagtt
     4741 agatatgaaa caaggaaagc taataactta gaaaatatga caatggaaga agcttttcca
     4801 gatgaaaatt ttagaaaagt aatttgtgat aaactaagta ttttagatga tggaaatcca
     4861 attggaattt cacaaaaagc gattatagaa tctacaaaag gtttaacctt agggagcaaa
     4921 tcaataaaaa atttatctgg aattaattat tttattggat tagaaaattt tagttgtaga
     4981 ggaaataatt taacgagtct agatttaagt agcaacataa atttaaaaga attgtattgt
     5041 aacgaaaatc aattaacaag tctagattta agtaataata gagagttaac aacgttgcat
     5101 tgtggagaaa ataatctaac agacttagtt atagaaaatt ctaaattaga cgaattgaac
     5161 tgtagaaaaa ataatttaaa gagtttagat ataactaaag caactaattt gacaactctt
     5221 ttatgttttg aaaatgaatt gactagtttg aacttatcta agaatcagaa attaaaaatt
     5281 ttgaaatgtg attataataa tttaaatgat ttagatatat ctacaaattt agaattacag
     5341 gatttaaact gttcttttaa tcaattagaa aatataaatt tagataataa taaagaactt
     5401 gttaagttaa tttgtcacaa caataaattg caaaatttaa atttaaaaaa tcatgaaaag
     5461 cttgtaaaac tatattgtaa tcaagaacaa ttaagtactt taaatgtgga aaattgtatt
     5521 aatttggaag atttaacttg tagtagaagt aatctgaaga atttggatat aagtagcaat
     5581 aagaatttaa aatatctaga gtgtgtagca aacaagttaa caaatttgaa tatagagaat
     5641 aatatagaac ttttagaatt atactgttat gacaatgaaa taaaagcttt agatataagt
     5701 aaaaatattg acttagaaaa tttaacttgt tataaaaata aattagatag tttaaataca
     5761 agtaaaaata aaaatcttaa atacttatat tgttcacaaa atgaattaac tagcttggat
     5821 gtaacaaaaa atacagaact tgtaaggcta tattgtgggg aaaatagatt aaccagctta
     5881 gatgtaagca aaaacacaaa attaaaagaa ttagattggt caaatcaaaa aaaatcaaca
     5941 tcaacaggag gttcgtcagg gagtggagga ggttcatcat ctacaaatga agagtcctct
     6001 gaacctactt caacaccatc taaggaaaaa ttaacaggtg tagacagaaa tgaaacatca
     6061 gtgaaaataa gtcaaaaagg atggaataaa gctgataata tagtgttaat aaatgactct
     6121 agcatatcgg atgctttatc agcaacacca tttgctaagt ctaaagatgc accaatatta
     6181 cttactaaaa ataataactt aaataaatta acagaaaaag aaataaatag attagaagca
     6241 aaaaatgtgt acatagtggg cggattaaag tctgttgatg aaaaagtagt gtctgattta
     6301 aagaaaaaag gattaaatgt aattagaata tctggaaatg atagatatga aacatcaata
     6361 aaacttgcaa aagaattaga caaaaattct aacctgtcaa aagtagtagt ggtaaatgga
     6421 gaaaagggac tagctgatgc agtaagtatg ggagctatat cagcaaaaga ggaaatgcca
     6481 attcttctta ctaatcaaaa tgatgatatg aaagatataa aagacttaat agctaataaa
     6541 aacatatcaa aatcatatgt aataggtgga gaatctttat ttaataataa agaagtgaat
     6601 aatacattac catctgtaac taaaatagca ggttctgata ataataaaga agtgaataat
     6661 acattaccat ctgtaactaa aatagcaggt tctgatagaa cagaaactaa ctcaaaagta
     6721 ataagtcatt tctatagcaa agatacactt aatgacttat atgtagctaa gaatggaatg
     6781 aataaacaag acgatttagt agatgcctta tcagtaggag tgcttgcagg taaaactgaa
     6841 tctccagtag tattagttgg aaatggattg gataatagcc aaaaagagtt aatcaagaat
     6901 aaaaagttta aaaatataac tcaaattggt ggaaatggaa atgaaaaagc atttaatgaa
     6961 gtagaaaact tagttaaata gattgaaagg tagtaactgg aaatagttac tacctccttt
     7021 ttttattcat tttcagtttt ttcaataatt atttttccat cctcaaaagt aacaagaaca
     7081 tccctttcat caggagagat attcatctct ttaatccatg acattggaag tatcaatctg
     7141 ggtgtaaaag agcctttgcc acttttatta aatccaattc taagttttct ttgctccaat
     7201 aatatcattc ctttctacta ttaataaatg ccagcaataa taaagtaaat accaaagcaa
     7261 taagagcttt aaaagtaaaa cttattatta tattaataat atttaaaact atagtgatac
     7321 caagaataat catacaaatt ctataaaatt tattattcat attaaattag aaaatgtgtt
     7381 ataatattag aaagaagcta cctagaagga aggtattttc ctcctaggat tgaaacttat
     7441 aattctttaa tcaatcttag tattgcgagt actagattaa ttaaaactaa tgtgaaagag
     7501 aaagcatttt taattttatt aaatttgttt tctcttttat tttttcttct ttctaatctc
     7561 cccactttct tacctccttt ctatatttat attatatcat cacgtgacgt gaaagccaat
     7621 aaatattcca tatttttcaa taaagttata aaatatatta atataaaata caaaatataa
     7681 taaaaataag tggatgttta cattatacac ccacttattt tagagttatt cctcatcaga
     7741 tttttttatt ataatctcat cttccttaaa aataactaca gccttacgtt tttcccttgt
     7801 aaatcccatt ttttcaagtt ggcttaaagg taaagaaatt ttagccgaat aagtacctga
     7861 accaccttta ctgtaaacta ttttagattc tttttgctcc aaactatttt cctccttttt
     7921 tatattgtaa tgtaacaaat attaacaata aaatgcttac tattaatcct attatattgt
     7981 tgatattaaa atgtaaaaat aaaagtatta tgtctattac tatagttata gcaactataa
     8041 taagacagaa tttataaact ttatcactca tattttatag aaaatgtgtt ataatattta
     8101 gaaagaagtc actaggaaga agattaaatt cttcctagct tacaaaacta taaatctttt
     8161 aataatttaa ttagtttatc aattaaattt attaaaatgg gtaagataga gagagcagtt
     8221 ttaactttgg cgagtttgct ttctctatct ttttttcttc tttctattct actcattttc
     8281 cttttcaccc cctttctata tttatattat aacatgccga accggcgaaa acaatattta
     8341 tttccatatt tttcaataaa gttatattga taaactatat tttttacaaa taaaaataaa
     8401 tggtgacaaa accaaacttg ccaccctttt gccaccgaaa ataaataata gaataaattt
     8461 aaattaaaca taataaaaca taaacaacaa aactgacctt aataaaacat tgaaatacca
     8521 acacttgtaa aaaataacaa caataaacaa ataacctaga ataatattac aacttaagta
     8581 aataataatt ttgaataata atttacggag gtattcatat gtttaaacac gataaagcat
     8641 tattaaaaga agtaaaagta gaaagaccaa atcctcaata tgctgtttta atgcaagaac
     8701 agttgggtgg tgcaaatgga gagttaaaag cagctatgca atacttgtcc caaagtttta
     8761 gaataaaaga ccctcaaata aaagatttat ttcttgatat agcagcagaa gagcttagcc
     8821 atatggaaat ggtagcacaa actataaatt tattaaatgg acatgatgtt gattataatg
     8881 cagttaatac aggcgaaata gaaacacatg tattaactgg tctatcacca gtcttaataa
     8941 actcatcagg agctccttgg actgctaatt acgttacagt tacaggggat ttagtagctg
     9001 acttactttc aaatatagct tctgaacaaa gagctaaggt agtttatgag tacttgtata
     9061 gacaaataga tgataaatat gtaaaagaaa caatagattt cttacttaat agagaagaag
     9121 cacataatgc tctatttaga gatgctttaa ataaagttaa agatacaggt tcaaatagag
     9181 attttggagt tacagaagat tctaaattat atttcgattt gtctactcca gggccaaatc
     9241 acgatactaa gatagatatt aatcctcctt cttttgaaaa acctttaaaa aaataatttg
     9301 atatttttta ttttgtttta gtagttataa aaattattta ctagaagaat taattatatg
     9361 aaaaattaaa tatgtatagt taaaaatggg tatacactta ttataagtgt atatctattt
     9421 gtttattgca aaacatataa taaaataaca aatgaggtgg tattttggac agtaatagaa
     9481 aacctaataa tctaataaat gaaaaatctc catatctttt acagcatgct tataatccta
     9541 taaactggta tagttggaat gatgaggctt ttaaaaaagc aaaagaagag gataaaccaa
     9601 tatttttaag tgtaggatat tcaacatgtc attggtgcca tgttatggaa aaagaatctt
     9661 ttgaagatga agaagttgct gaaataatga atagaaactt tgtggcaata aaagtagata
     9721 aagaggaaag acctgatgta gatagtgtgt atatgacagt ttgccaagct atgacaggta
     9781 gtggtggatg gccaatgacc ataataatga caccagataa aaaacctttt tttgcgggaa
     9841 catatttccc aaaatattca aggtataata gacctggagt tatagattta ttagagaatg
     9901 tatctgaaaa gtggaataca agcagagata tattaataaa atctggagat gaaataatag
     9961 aagctcttaa ggatgatttt ggtgtaaaaa atactgaggg tgatttatca aaagagatgt
    10021 tgagttcatc agttagagta tttaaggcta tttatgatga aaagtatggt ggttttggta
    10081 atgctcctaa atttccaagt cctcagaatc ttatgttttt aatgaaatat tatagtatag
    10141 aaaaagacaa agatgtgtta aaaatggtgg aaaaaacttt agatggaatg tatagaggag
    10201 gcttgtttga tcatattgga tttgggtttt caagatactc tactgataaa aagtggttgg
    10261 ctcctcattt tgaaaaaatg ctatatgata atgctatgct tactatagct tttttagatg
    10321 cgtataaaat aacaaagaaa gagttgtata aagaaattgc tataaaaact atagattatg
    10381 tagttagaga gatgaaggat aaagaaggtg ggttttattc agcacaggat gctgatagtg
    10441 aaggtgaaga aggtaaattt tatacattta atcctcttga gataattgaa gttttgggtg
    10501 aagaagatgg aattttcttt aataattatt ttgatataac tagtagtgga aattttgaag
    10561 gcaaaagtat tcctaatctt ataaaaaata aagaatatga gagacataat gaaaagatag
    10621 ctgaccttag taaaaaggta tttgaatata gaaaagaaag aacatctttg cataaagatg
    10681 acaagatact tacatcatgg aatgcattga tgatagttgc tttaacaaag gcatatagta
    10741 ctttaaaaaa tgacatatat ttagagtatt caaataagtg tcttaacttt ataaataata
    10801 atcttgtaaa tgagtcaggt agattattag ctagatatag agatggaagt tccgactatt
    10861 tagcatattt agatgattat gcttttttaa tctgggcgta tatagagctt tatgagtcaa
    10921 catttaatat gaaatattta gaaaaggctt taaatttgaa tgaaagctgt atcaaccttt
    10981 tttgggatta tgaaaaaagt ggattttata tatatggtaa agatagtgaa aatttaatag
    11041 caaggccaaa agatttgtat gatggagcta ttccatcagg aaattctgtt caattataca
    11101 atcttataag acttgcgaaa attactggag ataatagact tgaagaaatg tcatataaac
    11161 aattaaaact gtatgtggat aatgtaaaga gttctccagc tggctacagc ttttatatgc
    11221 tttcattgat gtttgaactt tattctacta aggaaattat atgtattttt aaggaagatt
    11281 ctgatttaat tgcctttaaa gaattgataa gtgaaaattt tataccaaat gcaacttttt
    11341 tagcaaagaa atacaatgaa gaaaacacta ttataggttt tttaaataac tatagattga
    11401 aggatgataa gattagttat tacgtgtgtc aaagtaatag ttgtagtcaa ccaattaatg
    11461 atttacaaaa attaaaagat atgattttag gagtagaata atcataatag tgaaaaagga
    11521 gatgctttaa aataaaagtc taatcatctc ctttttttgt acaaaaaaca gaaaaagagt
    11581 tcctgagaac acctaatcta tgtaatgatt gttttgttaa aaaataaaaa tgacattata
    11641 gttatataaa atattgtact ataaaaaagt agtattatag atgcgatagt attaatcgtt
    11701 tttatttact ctctttcttc tttttgcaaa ttcttcaagt gcttcagatt taattttatc
    11761 tatttcttca tattccataa aattagcatc ttcttcaaag ccatcttgtt cttcaccatt
    11821 aaaacgatac atttttttta gtaaactcca taaggtctct aattcatctt tggtaaattc
    11881 gtgaaataca tctgccataa agtttattcc agtcctacta catgttacca taaccttttt
    11941 tcctaagtct gtaactttaa cattaattgc tctcttatca tgaggacttg gaattacatc
    12001 aacatatcca tttttttcaa gattagcaac tagtctattt atattttgtt tacttgtacc
    12061 catttttctt gcaatattat ttaatgttgt ttcttcctct ggtaaatgaa gaattgataa
    12121 aatagtcata tactgtcttg aagtaagtat accaaaatat ttatctcctt ctatttgtat
    12181 tttgtttgta agggtaaaaa gagttgcata tgtttgattc attaaaaaga gttcttttag
    12241 ttcatttgaa taatccatga aatatatctc ctttattttt agatgttgtt ttgtcgaaaa
    12301 cagaatatca tgaattaatt ctaatgtcaa tatggtgtca aaatatatta aagtattact
    12361 tttttatgta actattaatt aattaaatta ttgtataatt ttatgtaaaa acttctatgt
    12421 accaatgata acttctatga ggaattaata attcttaaat tttttagaaa gattttgact
    12481 tgaaaatact tgtaatataa tatataaatt aatatactga ataaaatcaa tctggaggga
    12541 taattatgaa gtgtacaaac tttactttca aaggagagga aggtttagat atatatacat
    12601 ataaatggga agatgaaaat ataaaaaaac caaaggcagt tatccaaatt gcacatggaa
    12661 tggctgaaac tgcacaaagg tatgagactt ttgccaaggt acttactaaa aatggttata
    12721 tagtatatat caatgaccat agaggtcatg ggaaaactgc aaagataata gaaaatgttg
    12781 gacatttagc tgaaaaagaa ggatttagat gtcttgtaga ggacatgtat actttaacaa
    12841 atattataaa gaaagaaaat gaagatctgc caatttatct atttggacac agcatgggtt
    12901 catttgcaag tcaaagatat ataatggatt acagtaataa tttatctggt cttatactat
    12961 gtggttcaaa tggaaagcaa ggaatcattc taaatcttgc tcatctgata atcaatcgtg
    13021 agattaaaaa gtatggaaga cgttctaaaa gtaataaaat aaataatttg atatttggcg
    13081 gcgaaataat aagaagaaat gaaaagacta agtttgactg gttaagtaga gataaagaac
    13141 aagttgaaaa atatataaat gacccatttt gtggagtagt gtgtagttgt ggcttttttt
    13201 atgatttagt tcaagggtta aaagaaattg aggataaaga aaacttaaaa aaagtgccac
    13261 ttgatatacc tatttacata atatctggag ataaagaccc cataggaaaa aatggtaaag
    13321 gagtcttaag actaagggat agatatataa aattaggagt aaaagatgta acttgcaaac
    13381 tttacaaaga tggaaggcat gaattgttga atgaaataaa tagagaagaa gtttttgagg
    13441 atataatttg ttggttaaat aataaaatag aaatattgta ataaaaaaat ataatgatat
    13501 atttacttta aatttattta tatataatat ttgaaaagtg gattatgtat atgcaaagta
    13561 gaaaagagag agcccagaag tgattttttg ctataatgcc atttattgaa taggtgggtg
    13621 ttattataaa taaattatta acactcagta ttattatcat tgtatatgga atttatttta
    13681 tttaaaattc cttgaaagtt taaatatagt aagttgtagt aaagagaaaa tataaatatt
    13741 taaatgctat ttaatgtgaa tctattaatt aggttatact taaacttaat taatagattt
    13801 tatatttcaa gacaatgaga tagggtttta gattaaaata taaccaatgg ttaaattaca
    13861 gacattttgt taactttccc tcgatttact agtaaatatt ttttttatat acttaaatta
    13921 caacagatgt actagggagg tatatatgca aattaaaata ggtaaagtca tacagtgtct
    13981 tagaaaagaa cgaaatttaa ctcaagaaca attagcaaag tttataggtg tgtcaacacc
    14041 agcagtatct aagtgggaga gtggaaattc ttacccagat atagaattat tgcctttatt
    14101 agcagacttt tttaatgtgt ctattgataa gcttttaaat tacaagattg atttaagtga
    14161 agaagaagta atgaaaattt ataaagaatt agaatcaggc tttgctagaa ttgaaattga
    14221 tttatcaaca gaagaaccaa aagaagaatt tagacaagat ttagaatctg taaaaaagct
    14281 ttccaatatg tatatagaga agtatcctaa aagctattta ttgaagctaa ggatatgttc
    14341 gctgtatcag atgtattcat ataaatttgg taagagtgaa cttaatgaca gagtaaaaga
    14401 aacaacgaat attcttgaag atatagtgag aaatacagat gatatccaaa taaaagaaac
    14461 agctcttatt atattatcaa acgcatattg tatgttggag gattatgaaa aggcagagtt
    14521 gtatttaaat atgatacata agtcaatagg ggatacaagt gttaatttag ctatgatata
    14581 tttaaagcaa aatagacttg aagaagcaga aatattgctt caaaataaat tgtttagtaa
    14641 tgtatttaat ataagcatgg attgtaaggg aataattaat gtatacaaaa atcaatacaa
    14701 ggaattaaag aaaaaattgg agaatagaaa ttttaataaa aatgcaattg agagagaaat
    14761 ggagtatata aaaaataagt tgctggggta tgcaaatctt tctttagaaa taaaaaaaat
    14821 gctcagtgaa gataaaggtg cattttttag tatgtatatg gattatatgg aattatctct
    14881 aattttttta ttttttaaca tgaaagaaga agcaaaaaaa gctttatata gtctaaaaga
    14941 aatattagaa aaatatccta tacatgaaag tttggatgta agtcaaatga gattttttga
    15001 taaggtagag tctaaaaatt tatatacttt taatatatat acaaacttgt taattgtact
    15061 taatgatgac agttataatg agcttagaga agaaccaatt tttaaggatg taattgaaaa
    15121 gattttagat atggaaaaaa tgttgaaaaa taaagaataa gtaaaaattt aatataggac
    15181 ttaaagatta atcaaattaa aagaaagtgt ttcaaaataa aaatttgaat cacctttttt
    15241 gtcggtctta aacagttatt tagaaaaata gaggtttcaa aatagaaatc tgaattatct
    15301 gtattattac acaaaaatag aaacctaaat ttataaaaat agaataaaat ttgttatata
    15361 aaatgtaatt ttgtctataa aatctcacct gttttcttaa ctttaagcgt gaaaaagagg
    15421 atgtatcatg aattttttag ttcatgatgc atcctgcttt gtatgaaatc caatatatct
    15481 ttattattta aacaatggaa aagagtctta tttatatttt gagacatctc ttttatttaa
    15541 ttatgaagtt ttatacagtt caataaatag gaattatatt tagaaactca tgacattatt
    15601 tcttagatag tgattaaatt tatcatattc cattttagat aacttttctg ggtaagacat
    15661 tacaatcagg ttatgcccat taaatttttt atggtatggt ggatgaatgt gttcataatt
    15721 atttgtctta tattttactc tttcatagaa cgccttgcga taaattgaaa tctcatcaat
    15781 tggaaggtca atttctaaaa taatagtttt tcctttctta tttaaaagct ctaacgctct
    15841 ttgcccatat ctattattgc gaatttctgg aaaaatgcag aaatgttcta catagataaa
    15901 gttatctgtt tcccaacata aaattatgcc gacaaactgg tttttatcat aaattaaatt
    15961 gaattgatat tctttatttt tcaaaatttc tgcttgcaaa gaagattttc tttgtttatg
    16021 aaatgggaaa ctaattttat ataattccat agccttgtaa tataatttat tttcactggt
    16081 tcttaaccgt tcaaatttca ctttactcct cctaatcacg aaaattccaa tttatttatg
    16141 agttcatttt accatagtca gtatttgcat acaatgtgtt ttaagttgag tgtcatttct
    16201 ttcttttatt tatgcttata tctcatagtc taagagataa ttgcttaaga cataactaaa
    16261 aactattata ttaagctttg ataatctaaa aacagtatat ttatggactt ttctaggtct
    16321 aaaagtaatc ttaataattt tagaagttac aaaaaagtta cataaatttg gaaagctata
    16381 atatacatat ataatataag gttaaaactg taatgaataa tattaattta aaatttgaac
    16441 atggaaggag attattatgg agaaaagaga aaagaggata ataatatcat ctttattaag
    16501 tgtatcaatt ttaatgggtt tggcaagtat atattctata ttaaataaag aagacataag
    16561 tttaaccgtt aaaggtcaag agcaaaaagt atcttccttt aagaaaacag ttgaggaact
    16621 tttggatgaa cagggtgtaa agtataattc tgaagataag attaatccaa gtttagatac
    16681 agagttaaaa gatgatatga aaataaaagt tgttaaggta actaaaagta aaaaagaaga
    16741 gattgaaaaa attccatttg atacaaagca tgtaaatgat agtaatttgt taaaaggaaa
    16801 atctaaagtt tatcaagaag gtcaagaagg agaaaaaaaa ctagtctata atttaactta
    16861 ccatgatggc aagttagtca aaaaagtctt atcaaaagaa gtaatatcta aggagccaac
    16921 tacaaaaatt ataaaatatg gaactaaaga aaaagtacta atagcatcaa gaggagcaaa
    16981 tataagagga ggcaaacata tgaaagtggt tgccactgca tatgcaggag atacaataac
    17041 atctactggt acaactccaa gatggggtgt tattgcagtt gacccacgtg taataccata
    17101 tggaacaaaa gtatatatac ctaaactggg tatgactttt gtagcagaag attgtggagg
    17161 tgcaattaaa ggtaatagaa tagacatttt tatgaacagt gaaggaaaag cttcaaattg
    17221 gggaaggaaa agtatagata tatatctaca ttaaattagt gaatagaata actttacttg
    17281 aaggacaaaa aagtataaaa tatgaattaa ctaaactgtt agattattct taaaacataa
    17341 aaatatatct aaggggggat aagatgaaaa tagtctgctt aggagacagt ttaacgtatg
    17401 gatttggagt atcacgaagt aattcatgga caaacattgt aaataaagag actcgattag
    17461 aaatagtaaa taagggtata aatggtgata caacaagtgg tatgttagct aggtttaatg
    17521 aagatgttgt aaaaaactct cctgatatag tttttataat gggtggcacc aatgatttta
    17581 ttgctggtgc gggaaatgaa attattaatt ctaatataat ggctatggtg catcagtcat
    17641 attcaaaaaa tattatacct ataataggta taccattaaa accagatatt ccaaatgtta
    17701 gggaagattg gagttgtttt actgatttta atatagtatc tcaaaactta gaatcatata
    17761 gttattggat aaaaaaattt tgtatgacat ttaatactaa tttcgtagat ttttattcag
    17821 aatataataa aaatatggaa ctagaaggat ataaaaaatt atattttgat gggttacatc
    17881 caaccaaaga gggtcataga ataatggcag acatttttat taattcaatt aataagtata
    17941 tagaagagtt atagattaca ttaaaatgct tgtttatgcg tcagttgcaa taaatacata
    18001 cagtagagag gggtttatta tggattatac taaaacttat gaagagtgga taaagggttc
    18061 atattttgat gaagatacaa agctagagtt ggagaatata aaaaataatg aaaaagaaat
    18121 agaggataga ttctataagg atttagaatt tgggactgcg ggtcttagag gaataattga
    18181 agcaggaacg aatagaataa ataagtacac agttagaaga gctacttttg gattggcaaa
    18241 ctatatatta gaaaacacaa caaaagaaga aacaagtaga ggagtagtaa tagctcatga
    18301 taatagacac aagtctagac aattctgtat agaatctgca aatactttgg cagcatgtgg
    18361 tataaaagca tatatatttg atagtttgag aactactcca gaattatctt ttgctgttag
    18421 aagcttaaat gcaatcgctg gtatagtcat aacagcaagc cataatcctc cagaatataa
    18481 tggatataag gtatactggg aagatggagc acaagttatg ccagagattg caaatgcaat
    18541 aacagagaaa gtaaatagta tacatgatta tagtacaata ccaaccttaa cggaagctaa
    18601 taaaaatttg gtagttttgt tggacgaatc acaagatact aaatttatag aagcagtaaa
    18661 aagccaaatt ataagaaaag atttagttaa aaatgtaggt aaaagcttta agatagttta
    18721 tacacctctt tgtggaacag ggaatgttcc aataagaaga gcattgaaag aagttggttt
    18781 tgaaaatata attgtagttc cagaagaaga aaatccagac ccaaattttg cagggcttga
    18841 ttatccaaat ccagaagaga aaaaagcttt aaatagaggt atattactcg caaaagaaaa
    18901 aggagcagac cttgtaatag caactgaccc agattgtgat agagtaggtg tagcagtaaa
    18961 aactactaca ggtgagtatg ctttacttac aggaaatcaa attggtggta tgctgacaca
    19021 ttatataata gaaggattaa aagaaaataa taaattaaaa gaaaatccta caataataaa
    19081 aaccatagtt acatcagaat ttggagcaga tatagctaaa gcaaataatg ttgatgtatt
    19141 aaatgttctt acaggtttta aatttattgg tgaaaaaata aaattatttg aacaaaataa
    19201 aaatagaagt tatgtatttg gatatgaaga aagttatggt tacttagttg ggactcatgc
    19261 tagagataaa gatggtgtag tttcttcatt gttaatatct gaaatggcag cattttacta
    19321 ttcaaaaggt atgagtttat atgatggctt aatagaactt tataaaaagt atggattttt
    19381 caaagaacag actatatctt tgactttaaa aggtatagaa ggtgtagaaa agataaaaga
    19441 aataatatct tattttagag aaaatcaaat tgattatata aatagtatta aagtagttga
    19501 taaaaaagat tataaaaatg gtatagataa tcttcctaaa tctgatgttt taaaatactt
    19561 tttggaagat gaatcttggg tagctataag accatctgga acagaaccaa aacttaaatt
    19621 ttacatagct gtaaaaggag ctagtgatat tgaagcagat aaaaagattc aaggtttgaa
    19681 aaagtatata gatgacatgg tcgaaaaatt aaaataaaat attattattt tataagataa
    19741 ttttgtaata atatttattt agctgaaaat tataatttac tttcattgag tagaatatgg
    19801 aaatgttagt agaattgtta ttttcagctt ttatttgtgt tatttttact taaataatta
    19861 tattatagaa tgaatctgag tataattaat attgtataat gaaattaatt tgatagaaaa
    19921 ggggatattt atgtttggta tagataagaa attgattgaa tatataattg caaaatccaa
    19981 ttacagtgaa ttaagttcaa agtcagcaga agtatctttc ttcttaatgt tgtccatatt
    20041 tcctttttta atatttacaa taagttctat agcttatata ccgattcttc atttaaataa
    20101 gtatatagct ttgtttagaa atatgatgcc agaaggtgct tttgctgtgt tatcttcaat
    20161 catagtttct gcaatagaca atagaaacct gaaattttta gcagtcagtt ttgtacttac
    20221 tatgtggaca ttttcaagag cagtaaaagc tcttattaaa ggaatgaata gagcctataa
    20281 agttaaagaa actaggtctt tttttaaaat tttatctata tcatttttat ttacaataat
    20341 gcttttggtc cttatttttt tatctatgat atttttagtt tatggggaaa aaataggtta
    20401 ttttattttt aatcttgtag gattggatga gatatttata aaaatatggg atattctaag
    20461 atacactgta ggtataataa caataatagt gatatttaca cttttatata aatacactcc
    20521 aaataaaaag ttaactatta aagaatctgc gccaggtgct atttttgcta cttttgcttg
    20581 gtttttagta tcatttttat attcttatta tacaaattac tatgctaact atgaagtaat
    20641 ctatggaagt atagctgaaa taattgtact tatgacctgg atgtatttta gtagttggtc
    20701 tatcgttatt ggctatgagg taaattcaag attgtatttt agaaagataa gacatgaaat
    20761 gttaaagtaa agtatttaaa ataaaagaga gtatttagta ttataaatgt aattgaatta
    20821 ataaagaaat attgacattt aagttaaaaa gtctagaatt tttctgggct ttttttattt
    20881 ttagtataac ttgacaaatt ttatttaatg ggatagtttt aatttataat catctttaaa
    20941 aagatgacta ttgataagta agagataaaa ttggtaagta tcaaaaaata tactaaataa
    21001 aaattatagt cctaatgagt taatttctgt attttcaaat aatgcagtat ttagtcatgt
    21061 gtttttatat gcgcctgtat ttctaattac caatatatta gaataagcta ctgtaattag
    21121 atttagaaaa aagcgacata aataattata aacaaataat aaattacata aaattaaatt
    21181 tattcacaag tatatcttat aaaatatgtt ataattaata gaaaattgtc atataattaa
    21241 ttaagtagta ttattataaa taacattgaa aggtagtggt tagaatatgg atacccataa
    21301 taaatatgta aattttataa aaaatatacc tgtacctttt ttatattgta gaattgtaaa
    21361 gagacaagaa gatatagaat atcgagtaga atatataagt aagggtatgg gcaaagtatt
    21421 acaattagaa gaaggtattt gtgataaaaa tatactagat gtattgcctg tatttaagtc
    21481 taaaaaatat ttcaaagagt tgttctcaaa tgaagtggat tgtataaaaa ggtacattcc
    21541 gacacttaaa aattggataa atataaaaaa acagataata ggggattcat acataatttt
    21601 gtattttggt aaaattgtat ttgattatag acagataatc gattcctttg ataaaaagga
    21661 aaaagtagct tatattaaag acgaagaagg cacatatata gattgcagtg aaaatttaat
    21721 accaatatta aataacaaca ttaaaacaac gaaagatatc tttggtaaaa atgatataga
    21781 agtatgggga gagaatacag gaaaattatt tagagatgat tatagagaag gagtatctag
    21841 taaaaaaaga tttttacaaa atctttttga atatgaagaa acatttttta tggttgaaaa
    21901 atattttttg tatgatgaag acgaactttt aggtactata gggatagtag acaatataat
    21961 atatagtggt tacagtaata gaaattataa ctcaaaagat ttaatgaaga tgatagaaca
    22021 ctcaattcca gagaatatgt tttataaaga tgtgtatgga aattatattg gatttaattc
    22081 aggttttttg aatttagctt gtatgaataa agaagaatta ttagggaaga atagttataa
    22141 aatatcagaa gaagaagctt taatagataa gatatttgaa agtgataagg gtgtagttga
    22201 aaataaaaaa gtagttacat ttgagttaaa tataagtatg aatgatgaaa acaagtgtat
    22261 tgagattaca aaaaggccgt tttttgatag ctatggaagt gttattggaa taattggaac
    22321 agcaagggat ataagtagaa gaaaaagatt agaagaggaa atggataaaa ctaggatgga
    22381 attttttgca aatttatccc atgaacttag aacgcctata aacttaatat catcttcatt
    22441 acaagtgata gaaaaaaaag aagcagattt aatagaatca aatgatactt taaaaagaaa
    22501 tttaggtata ataaaacaaa atggaaatag aatattaaga ttagtaaata atgttataga
    22561 ttttacaaaa atgcaatcag gatatttaga ttttaaacca gaagaatctg atataattgc
    22621 atttatagag gagatatgta tgtctgtagc tgattttgca agccaaaata atatacaact
    22681 tacatttgat acagaaatag aagaattttc aatgttattt gattcagaaa aattggaaag
    22741 aataatatta aatttacttt ctaatggaat taagtacaat aagaaagatg gaaaaataaa
    22801 tatattttta tatgtgaaag ataatgtatt taatatgaaa atatctgata gtggaatagg
    22861 aataccaaaa gaaaaaatag ataaaatatt taataggttt gaacagattg acaatgaact
    22921 ttcttacaga gttaaaggaa gtggtattgg actttcatta gtgaaatctt tggttgagtt
    22981 gcatgaggga agtatatctt taaaaagtca acttggtatt ggaagtgaat ttatagtttc
    23041 attgcctgtt agaagtaaaa ataatattga aaaatataat cataaaagag aaatcagcaa
    23101 tgaattaagt aaaaaattag aaatagagtt ctcggattta taagtttaaa tataaaaaat
    23161 aaaattttaa aaaagtattg acaattgatt tagaagggtt taaaataaat tacaacataa
    23221 taaataaaaa ggcgatgaat aggaagagta aatctaattc gattaccaga gagaagagtc
    23281 cgttggctgg aagacttttt aaattagagg gattgaaaac tacctaggag ctactttctg
    23341 aagtcatctg cattagtaga ttttagtaag aatagtcgtt agctgcgtta aagtgtaaag
    23401 tgagttttca tgacatatga aaactaaatt aggtggaacc gcgggaataa tactctcgtc
    23461 cttatgttat aaggatgaga gttttttgtt atttaaaaat ataaacagta atcataataa
    23521 ttatagaaaa tataaaagca atgaatagga agagtaaatt taattcgact atcagagagg
    23581 aaagttcaca ggctgaaaga ctttctaagt ttgagaaatt gaaaactacc taggagctgt
    23641 ggtctgaagt tactttaaaa agttttagta agactaaccg ttaactgcgt taaagtgtaa
    23701 agtggatttt atcaataaat gataaaatca aattgggtgg aaccgcgagt atagactcgt
    23761 ccctttatat agggatgagt ctatttttat tgcaataaaa gattgaaaat aaaaaaaaat
    23821 cggaaaatta taaatttagg aggaaaataa aatggagaat aaagtgaaaa ttggagtact
    23881 aggatatgga gttgtaggaa gtggacttat agatataata gataataata aagaaaaaag
    23941 aaatatagaa atagttggaa ttttagttaa taatttggaa aaacataagg ataaaaaata
    24001 ttctaatata ataactaata atatagatga tatatttaat aaagatatag atatacttgt
    24061 tgaagtcatg ggtggtttag aaccatcatt atcttatata aaaaaagcat taaacaataa
    24121 aattcatgtt gttacagcaa ataaagattt gttagctgaa tgtggagatg agcttgcaaa
    24181 attagcaagt gaaaataaag taagtattaa atttgaagct tcagttgcag gaggtatacc
    24241 agtattaaaa ccaataatag aatcactaga aggaaataat atagatagta taaatgcaat
    24301 tttaaatgga actactaatt ttatattatc taaaatgtat gatgagaatc tatcttatga
    24361 tatggcatta agacaagcac aagaattagg ttttgctgaa gcaaatccag agtcagatgt
    24421 attagggtat gatgctgcta gaaaactatc aatattatca actctagctt atgacaatag
    24481 agtatattgg aaagatgtgt atttagaagg aattacagat atagatgaaa aagatattga
    24541 atatgcaaaa aaattaaatt gtaaaataaa attgattggt caaagtaagt atgaaaatga
    24601 taaagtaagt gcttttgtta gacctgtttt agtagaaaaa gataatatcc ttgctagaat
    24661 agacaatgag ttcaatgctg tcatagtgaa tggagattca gtaggagaag tttcttttgt
    24721 aggtaaagga gctggaagtt tagcaacagg aagtgcggtt tattcagatg ttatagatat
    24781 aatagataat agagtttcaa gtatagattc ttttactaaa gataaaatac aagttaataa
    24841 aatagttaga gaaaagtgtg gagcacttct tagatttaaa aaatgtaata aagatgaaat
    24901 tctaaatata gttgagaatt gtttagtaaa attcgatatt ttaaatgatg atgatgaact
    24961 tgctattatg gtttatgctg attctgaata tgaaataaac aacagtttat gtctaataaa
    25021 agataaagga tattgcgaaa aaatgaataa aatgttgaaa ataagttaaa aaagaaaaaa
    25081 tataagttta tatgtaacat attttgacga ttttaatact atatagtatg agatgaaaca
    25141 aaacatctca taaaaaaatt aaaattaaag ggagagatat gttatatgga aaataaaaaa
    25201 tgttattcag aagattggta tgaaagagga gaatctacag ctaaatggtt ccaaaatgat
    25261 agagaagaat atgaaagaga agcatatgat gaagatagag aaagaagagg ttcaaactgt
    25321 ggatgttcag attcaggaga aaatagacct agaaactgtg aaagatttag aagagaagct
    25381 gagataagag aaagagaagc aagagaagca ttctgtgaat cttcagagaa aaagaaagag
    25441 gcattagcat atgaatgtga agctagaaaa ttatgggaag aagcagaaaa atactgggat
    25501 gaatattcaa aatacaacta taaaggaatc gaatatttag cagaagctgc tagattattt
    25561 gatgaaggta tggaatgtga agctagaaga aatggaaata atggaggaaa caataataat
    25621 tgttgccata aatgccataa atgtaattgt aactgctgta gaaaataaaa aaattaattg
    25681 acaaatcaat agttaaaata tatcatatta tgtataataa agagaaaatt cagaaaaaaa
    25741 taatgattag aagaaatata agtatattta ttagacaaat agtgctcagt tagtgaggtg
    25801 agtacaatat gattaaactc ccatcctcta gcttttgata gagggtggga gtttttattt
    25861 atttatatat cttaaatgag gataatattg taaaataaat gagtatgaga ttgtaaaatt
    25921 aggaggtaaa aatgaaaagt attaaaacat caagtatgaa agaaaaggta gatgaaaaga
    25981 tattaagaga gcaagtaagt tctataatag aagatataag aaataataag gatgttgcac
    26041 ttaaaaaata taatgagaag tttgatagaa atacaagaga tgaatttaga attactaagg
    26101 aagaaataaa ggaagcttat aagcatgtag atgacgaatt tataaataac ttaaagattg
    26161 ctgccaaaaa tataagagaa tttgctaaag ctcaaaaact aagttttgaa aacccttttg
    26221 aaaaagaaat ttatccaggt gtaatcttag gtcaaactaa tattccaata gagtcatgtc
    26281 ttgcatatgt tccaggaggt cagtatccat tgttttcaac agctcttatg cttataatac
    26341 ctgcaaaagt agcaggagta aaaagaatag tggcatgttc accaactatg aaaaatactg
    26401 aaaagataaa tcctaagaca ttggtagcta tggatatagc aggtgctgat gaaatatatg
    26461 caactggagg agttcaagct atagcagcat ttacatatgg aacagaaaag attaatccag
    26521 ttgacataat tgtaggacca ggaaataagt ttgtaactga agctaaaaga caatgttatg
    26581 gacaagtagg gattgatttt gtcgctgggc caagtgaagt tcttataata gcggatgaaa
    26641 cttcaaatcc agtttatatt gcagctgatt tattagcaca atgtgagcat gatttaaatg
    26701 caagaggaat attattgaca aatagcttgg aagtagcaca aaaagttgaa aaaaatatag
    26761 aaagtatgct aaaagatttg ccaacaaagg acatagcata tagttcatgg gaaaataatg
    26821 gagagataat attggttgat gatatggaag aagccattaa aataagtaat ttttatgcac
    26881 cagaacattt agaagttgca gtaaatgagt ataatgatat ttgtgataga ttaactaact
    26941 atggttcttt atttattgga aatctttctg ctgaggtgtt tggtgactat gtttcaggta
    27001 ccaatcatac attaccaaca cttaaagcat caagatatac tggtggtgtt tgggttggaa
    27061 cttttatcaa aacttgtaca aagcagatat ttaatgaaaa agctatacaa tcattagcac
    27121 ctgtagctga aaagctggca aaagaagagg gattatatgc acatgcaaag gctgctgagg
    27181 ttaggtttaa aaagtagtaa aaaattttat tttggcagtc gagacttagt attagactga
    27241 gataaggaaa acgctgagaa cctgaataaa tactatattt tgtgggtctc ggcgcttctt
    27301 ttatatctgt atataataga ataatactat ctgtgtgttt aaatactaac ttaatatttc
    27361 ttttctaaat aatgtttatg aaatagttaa tatttttagt taatacttga aaaaaacttt
    27421 tgctaactgt gcaaattcat ctgcatttgt caatatagca gggtgtcctc cctgactaaa
    27481 aatgtaaata cttgcttttg aaataaaagt tgccatttgc ttgtactctt cttcaagatt
    27541 atgacgacac atttcgtctt ccttacttcc tgtaaataaa attggcattt ctaatttaca
    27601 caattcttta tgaaacaaag ggcgtttctc gtttgcacat tgcaatagag cttttgtatc
    27661 aagattaaca acattctccc aatcttttcc atgacaccat tcatagaatt ttcgtgcttg
    27721 aatatcttgc tttgattttt cccttccagt gattagatta tctatgaagt tatcattaag
    27781 agtacgtcca tcaaagctat ctgctataat tgcttcaact aaatcgggac gttctaatcc
    27841 agtattaaca gctgcccatg ctcctccact tgttccaatt aagccaactt taccacaatt
    27901 caaatgttca atcagtgcta ttgtctgtaa ggcttcatca tgccatatgt caggagaaaa
    27961 ttgagaaact ctatccgact gtccattacc taaaaaatca attaagatac atctaaaatt
    28021 ttctgcatat aatggcacaa gtgctttaaa catattagaa gaaccagtat taccatgcag
    28081 gaaaattatc ggtttacctt gtccatattc tttataaaaa caagaattac cttgatactg
    28141 aaaatatgac atatattttc ctccttttta tttgttaagt taattataac aatataggta
    28201 tgaatgaaaa acctcatgtt ctttcttaga ataaaaatgt gccacgcata aaacgtgaca
    28261 catttctaat tataaatttt gctgtttaag tattctttgt atgcttttct ctgtcaaaaa
    28321 gtatttctct gataactgct tgatactcat tccattttgg aaatttatac agatttgaga
    28381 attacgagaa gctaagactt gtttggtgtt tgtgttttca ccccaatgct ttttgtgctc
    28441 ttgctttttg ggaatataaa tcactttacc atctatatat tcttgcactt gctcaatcag
    28501 ttctttaggc agtaagtcaa tcgaattttg atagctcatt ttgcacctcc taaataatta
    28561 ttctttagga ttagcaaagg ctactgccac tcacaattaa aaataaaatt ttttattatg
    28621 tacaatagcc tttgctatgc acaagttcca attcccataa actaaatact cctttctggt
    28681 ttgaaaaatt catctgaaca ttgatttcgc ttttaagata gagttcaatg acaaatttac
    28741 atttttatta tatatattca gatagataat tgcaactgtc tttgtatgag gatattttat
    28801 tatagtcaat actataatat gtatttatat catcaaacta atatgaacag ttgaaaggaa
    28861 tatgaacaat aaaacttagt caggaagaat aattatgaag cagacaatag agaaatttga
    28921 ttttaaagtt ttattgaaaa tttattgaca aatttaactt gtaaatatta tactaaactt
    28981 aataaataaa gtttataaag taaggtgttg tctatgaaaa atatagttgg aaaaccgcaa
    29041 acgataaaaa aagtaaatga agatttaata aaaaacatta taaaaaatga aggtcctata
    29101 acaaaaccag aaatttcaag cattactgaa ttgagtttag ctacagttaa taagattgta
    29161 gagcaattag ctttgaaaaa tgaagttaaa gtaagtggac taagtgaatc tactggagga
    29221 agaagagctc aattatttga aataaatgca aacttagaac acataatagc tttatattat
    29281 tatagaaatt gttgtatagg ggtagttgcc aatttattag gtgaaataat ttatcaagaa
    29341 gaatttaata taagaatgga tacgtatgaa aacgttaacc aagatacttt ttttgttata
    29401 gatactctaa taaaaaagga aaaaggaaac aatataaggg ctattggaat tggtgtccca
    29461 ggagttgtaa aaaaaggtgt agttaaaaat ataccaacta taaaaagttg ggaaggtata
    29521 aatttagaaa atttaataaa gagtaaatac aatactaaag tgtttataga aaatgataca
    29581 aatattacta ctattgggat ttatcaaaaa cagtataaag acaaatataa aaatatggct
    29641 cttatgtatt tagaagatgg tattggttca ggtatagtta taaatggtga attgtattta
    29701 ggttctacaa attttgctgg agaattaagt tatctaaata tagaatttgg taagtataac
    29761 tctaacaact taaatcttga agattatatt atgagtttaa gaaaaagata tttagaaagt
    29821 aaagataata aatttaagaa agaaatttta tctataatat caaactttat aaggactctt
    29881 gtatgtgtcc taaatccaca agctatgatt atacagtgta gtatattaac caagagtgac
    29941 atggagttta tagaaaaaga tgtaagcaat aatgttggag aagaaaattt tcctaaatta
    30001 ataaaagtag actcattgag agaaggaagt ataaatggag taattagtat gtgtttaaaa
    30061 gaaactgact atcaatattc cttatctaat aaaaaaggag gatattaatg tgaaatattt
    30121 ggtttcaata gatggagggg gaacaaaaac taaattttgt gtaagtgact tagatggaaa
    30181 catacttaaa gaacatacca ctggttcaac taattataag tcagttggaa ttaaaaaaac
    30241 atatgaaaat attaataatg gatttaaaaa aatattaaaa gatttatata tcgattatga
    30301 cgatattgag tacactgtat ttggtatatc tggttgtgat tctcctaatg attataagat
    30361 tataatggat gaaatattaa aaattgggat taacaaagaa aaaatatatc tggcaaatga
    30421 tgcagtttta gctttttatg cacaagctga ttcccctggc ttagtaattg tggctgggac
    30481 aggttcaatt atactgggaa ttaaagaaga tggagaaata tatagagttg gtggatgggg
    30541 gtataatttt agtgatttgg gttctggata tgatataggg agaaaattat taaagaaagt
    30601 attgctttat tgtgatgaat gtcatgagta tagtgattta tttagttgtg tcttggactt
    30661 ctttggagca aatagttttg aacaacttgc atatatgatt actgatataa atgataatgt
    30721 tgaaatagca aacttagctt cattagtgat tgattgtgcc gaacaaggtg ataaattggc
    30781 tattgaaata ttaagagaat catcaactga actatctaaa ttagctcaag taatattgag
    30841 aaaaatatct aatccaatga aaatggataa aagcgaaata aatatagttt tgtctggtgg
    30901 tacattaaat aaaactgtat atgctcaaat gttaatgaat aatttaaaaa cagaaaatac
    30961 agataaaaat ttaaaattta tatctcaaga aaatcagcca gtatatgggg ggataagact
    31021 tgctatggca ttagctaata ggagggtcaa gcatggatag aaaaaaggta atagtacaaa
    31081 tttttactgg aggttttaag gctaaagaaa taaagtttaa tgatataaaa tgtaaactac
    31141 tagaattatt ggactgtatt gatatagaaa aggttatcat gggatggagt gtagatgaag
    31201 aattatatgt agaaactaaa aaactattaa ataaatataa tgttgaacta tatctttggt
    31261 tgccatgttt ttctgaaata ggattacttg aaaaaagtaa tttactaatt gattatatgg
    31321 ggaaggaagt caagggctat gctttacaag aggatgaaaa ttttgagttt tattgcccaa
    31381 ataataagga aaatataaaa aatataaaga aaatatatag tacttatttt agtgatatta
    31441 aatttgatgg aattttttta gataaaataa ggtatggttc tttttcgaat aaactaaatg
    31501 gtgtttttaa ttgtttttgt aaagattgtt taaatatata taaaaaaaat aatattgata
    31561 aagagtcgtt aatgagagaa atgaataatg tgtctaaggg agttatagat aatgaagata
    31621 ctccatttgg tataacagaa tacattaatg gaaaatacaa atttaaaaat aaaatatggg
    31681 aagatttttt tgaaacaaaa aatcaaaata tatacaaggc attattagag ataagtagtt
    31741 attttaaaag taagggattg aaaataggta tagatgcttt ttctcctttt atttcttact
    31801 ttacaggtca agatttagaa aaattacaag aagtagcaga ttttataaaa ccaatgatgt
    31861 atagaactac aaaagctcca gctggtttac catttgaatt tgaaaggttt attgatgaat
    31921 ccatacataa gaataaagag attggaaaaa gaaatttctt agatatttta ggaattaagg
    31981 agtatgaagg agatgtatat cctatagaat ttgtactaga ggaaatcgat tttatgacaa
    32041 gaaatcttaa taaagatata tatgtaggag ttgaaataaa taaaaaggaa attatatctc
    32101 ctgttacacc taagtacata agtgaaaata tttcaaatat aaataagaca acagtaaaag
    32161 gatatgtttt atcttgggat ttgttgtcag caccaaaaga aaatattgat gaaattataa
    32221 aatggtttcg gaattagtga ggtgataaag tgagtaagat attggaaata caacaaatat
    32281 ataaaagttt tggaaaggta gatgttttaa aagggataaa ttttgagtta gaaaagggag
    32341 aagtaagagc tcttttggga gctaatggag caggaaaatc tacacttatt aaaattttag
    32401 gaggagttca ttctcaaact aaaggtaaag taattttgac tggtaatgaa ataatgttta
    32461 aaaatccaca tcattctaaa gaaaatggta ttagtataat ttatcaagaa ttaagcttga
    32521 taccaacgct tacagtactt gaaaatattt ttcttggtag agaaatttta aaaggaagtg
    32581 tgtttttaaa caagaaagac atgcttgagg aatatatgaa aatttgtgag gattttgatt
    32641 ttaatataga cccacatata gtagtttcta atttaagtgt agcaaaccaa caaatggttg
    32701 agataatgaa agctgtttca tgtaattctg aaattatcat aatggatgaa cctacaacat
    32761 cacttactaa taatgaaaaa gatgggctat ttaaaataat aagtagacta aagtctaaag
    32821 gtaaaacaat agtctatatt tctcacatat tagaggaagt ttttaaagta tgtgatagtg
    32881 caagtataat gaaaaatgga attatgattg gaacttatga tgtagaggat ttaactaaat
    32941 taaagataac tcaacttatg acaggaaatg ataatcacaa acttattgag aagaaggatt
    33001 atacatttgc aaaatatgaa gagccaccag tactagaagt aaagaatata agctggtcta
    33061 acaaaataaa aaatgtttca tttaaagttt atagaggcga agttgtaggt cttgcaggtc
    33121 ttgtaggttc aagaagaact gaaattataa atttgatatt tggtatagat aataaagata
    33181 gtggagatat ttttatcaat ggtaaaaaag tagacataac ttctcctaag gttgctataa
    33241 aaaacaaaat aggattgatt cctgaagata gaaaaaatct aggattaatt ttagaacaag
    33301 ctatatacac aaactcatca tcagtacaaa ttgataaatt caagaaattt ttgtttatag
    33361 atagaaataa agaaattgag ttttctaaaa aaggtgttaa ggaattagga ataaaagtta
    33421 gtgatgtaaa gcaagaagta aaagagctta gtggtggaaa tcaacaaaaa gtagtagttt
    33481 ctaagtggtt agagaaagat ttagatttaa taatatatga tgagccgaca aagggaatag
    33541 atattgctgc aaaagaagat attttcaata caatcaaaga attttcaagt aaaggaatag
    33601 gagtaatatt tatatcttct gatttagaag aagtaataag agtaagtgat agaattttag
    33661 taactagaga aggttctatt gtagatgatt taaaaaatga gaatatacag gttcaagata
    33721 taatgaacag tatatttaat gtataaaaaa tgaaaatgga ggactgagat gaagaaagct
    33781 tttgagaagt taaatttaag agattatgga gtattattag gttttttagc tttatgtata
    33841 gctataagta ttgcaactcc atcattttta ggtaaacaaa atatacttaa tcttttacga
    33901 caatcttcta taattggaat aatttcagca ggtatgactt ttgttataat atctggaaac
    33961 tttgatatat cagttggagc agtagcagct cttgcaggtg ctataacaat gaaatttgcg
    34021 actggtggaa ctaatttgtt tgcatccatg ttcttaggtg tagcgatttg tgcagtaata
    34081 ggattaatca atggtatagt agttgcaaag ataaatgtac catcattaat agccactatg
    34141 gctatggtca ctattgtaag aggattgctg ttgatgctta caggaggata tccaattact
    34201 gaaaatatac caatacttga ttacattgga aatggatatt tgtttaatat accaatacca
    34261 gtagtaattt tctttatagt ggtgttagct tcgtttattg tacttaataa aactaagttt
    34321 ggtagatatg tttattctgt aggagggaat caagaagcaa gtaaacttaa tggtataaat
    34381 gtagattctc acaaagtgaa agtgtttatt attaatgctg tacttgcagg aatagctgga
    34441 atcgtactaa caggaagact tgggactgca actgcgattg caggtgaagg atatgatatg
    34501 gatgcaattg catctgtagt aataggaggt acatcagtgg caggaggttc tggttctgta
    34561 cttaaaactg ttataggagt attacttatg agtgttataa acaacagttt taatcttctt
    34621 ggaatagatg tatatttcca gtacatattt aaaggattaa taattttagc agcagttgga
    34681 tttgattctt atagcaagaa aaaacttgct tcaagataaa aaataagggg gattttttta
    34741 tgaaaaaatt aatgaaacat ttagcattgt tgctatcact tgttatgata tttggtttag
    34801 ttggttgttc aaatggtggt gacaaagaaa aaaatggaga aaagaaaata gcagtattac
    34861 taccaggttc aacaggttac tttgtagcaa ctaaacaagg tatagatgca aaatctaaag
    34921 aattaggtgt aagcgttgaa tatgcagatg ctcaatggga tgctagtaaa caattatcac
    34981 aagctgaaga ttttatggct aaaggggtag acatgattgc attatgtgga gttgattctg
    35041 ctgtaagtga gagaatagtt aaagctgcta atgattcaga cgttcctata gtagcattta
    35101 caaattctat aggaagtaac ccaactggag aatttaaagg tttagttaca tatataggac
    35161 aaaatgagga agaaacagga gcattaactg gaaaaattgc taaaaactta ttaggagaaa
    35221 ctggtgggaa agctgtatta atagaaggag taccaggaac tactccacaa gtattaagaa
    35281 aaaaaggtct tgaaaaagaa ttaaaagata gtaatataga aatagtttat aatcaaactt
    35341 caaattggga gaaagagcaa gctttaaaag taacagaaga tttaattcaa aagaaaactg
    35401 attttaatgt tataatatgt caagatgata attctgctac aggtgcaggt caagctttaa
    35461 aagatgctgg tcttaaggat aaagttaaag tcataggttt gggtggaagt aaagatggat
    35521 taaaagctat aactgatggg ttaatagatg gtacaactta tatgtctgct gtagaagaag
    35581 gtggattagt catagaaaag gcatctaaat tccttaaagg tgaaaaaata gaacctgtta
    35641 cacaaattaa acaagttgaa gttaacaaag ataatatttc agagttcaag ggtgaatggt
    35701 aaattgtcat agataataaa taagattttt agagttactt tagaaactat ataaagtaac
    35761 tcttttttta atttaattta gtgataaatt attgaatagt atttctaaac aaataaaaaa
    35821 ttaatataaa caaagactta attatactaa attataagta aaactaatat aaacttatgg
    35881 aaaaaaggaa aacgtctaaa gtattggaat tactatagtg taggtgtagt attaaaaaaa
    35941 cataaaaaaa cataaaaaaa ttgttgaaaa aaatatagaa attttgtata attaaagatt
    36001 tgaaaagtgc ggtatacaaa gccaattctt ttgtatataa tatatataaa ttcagaaaaa
    36061 ggaagggata tgtatggcca ggcggttatt tttagaaagg attggactta tatttttagg
    36121 ttcagcaata ttagcatttg gagtttataa tttttattat ttaaacaaca taacagaagg
    36181 tggagtgtta ggtattttac tactgttaaa aaatttattc aatatacaac cagcaatagc
    36241 aaatgttgta atagatggtt tactactttt agtaggatat aaatttttcg gtaaaaaatt
    36301 ccttatatat tcaatagtag cttctataac tttttcagtg ttatatgact tatttgaagc
    36361 tataggacct ctagtaccac aatcacaaaa tatgctttta tcaactattt tggcaggtgt
    36421 aactgttgga actggtgtag gtatagtagt aaaagcaggt tgtgcatcag gaggagacga
    36481 tgctttagca ttagtaattt ctaagactac atctttgaac atagggcaag tttatttagc
    36541 aacagatgtt attgtattgt tgttatcatt attttatcta tcagcgtttg atatctttta
    36601 ttctttgatt gctgttacaa ttagtggcaa ggtaattgat tttatttatt atcatggtaa
    36661 aagtttggat atggatataa gtaatgatat agttccagaa tgttaatata aattaaataa
    36721 ctttagattt tattaggggt ggcagatttg ccaccttttt tgtgtctaaa tctttataga
    36781 aagtataatc ccatctaggt ttttgcaaat ctcaaatata gaattatgtt ggaatgactg
    36841 ggaatacgat tgctagatgt gcattttaaa aacccaattt tatatttatg aaatactaat
    36901 cattaatgga tgtttcttaa tcctatcttt atacctaaaa tgataagata aatgaagtag
    36961 caatacaaaa ctaaaatttg taaaagagat taaattaaat ggaggagcta aaatgttgga
    37021 tatagttatg atagcattgt tattcatagg atttgtttcc atgaagttac ttgctgattg
    37081 gtgtgggaaa caaactgaaa aaaaataatg taatgtatgg agggattacc tatgtggttt
    37141 ctggcagttg taattataat gttaataata tatttggtat atgcgcttat taatcctgaa
    37201 aaattttaaa cagtataagg ctaataagta agttgaattg gggggaacaa tttttatggt
    37261 gcagatagta atagtgttag cgatttttat gattttagta atacctatgg gcaagtattt
    37321 atatcatata gctactaatc aaaaaacatt tggagataga ctatttgata aagtggacaa
    37381 ttttatatat aaggtatgta gtatagataa gaaaaaagaa atgaactgga aacagtatgc
    37441 attagcttta ttatttacaa atgcagttat ggtatttata ggatatatca tacttagaac
    37501 tcaaagtatg catattttta atccaagtgg aattaaaagt atggaacaag gactatcatt
    37561 taatacaata ataagtttta tgacaaatac aaatcttcaa cattattcag gtgaatcagg
    37621 actttcatac tttagtcaga tgacagtgat aatatatatg atgtttacat cagctgcaac
    37681 aggatatgcg gctgctatgg catttgtaag aggtctagtc ggtaaaaaga aaacattggg
    37741 aaatttttat gtagatttaa ttaggataac aacaagagta cttttaccag gagctttaat
    37801 tattgggctt atattagtta cacaaggagt accacaaact tttgctggga cagaaacagt
    37861 gactactata gaaggaaagc ttcaagatat agctagaggt cctgtggcag ctcttgaatc
    37921 aataaaacat ttaggaacaa atggtggtgg attctttggt tcaaattcat cacacccatt
    37981 tgaaaatcca acaataatat caaatatagt agaaatatta tctatgatga tattaccagg
    38041 agcttgtgtt gttgcatttg gtcacatgat aaagaataaa aaacaaggtt gggttgtgtt
    38101 tggcgcaatg tcaatcatat ttttaatagg tctagtagtg tgctttaaag cagaaagtgc
    38161 aggaaatcct attttatcac aattagggct taatcaaagt atgggaagta tggaaggtaa
    38221 ggaagttaga tttggtatag cacaatcatc attattcaca acagtgacta cttcctttac
    38281 gacaggtaca gttaataata tgcatgatac actgactcct ttaggaggat tagtaccact
    38341 tttaaatatg atgctaaatg tagtatttgg tggtaaaggc gtaggtctga tgaatatgtt
    38401 gatgtacgct ataatagcag tattcttatg tggattaatg gtaggaagaa caccagagtt
    38461 tttaactaag aaaatcgaag gaaaagaaat gaagcttatt gctttactta ttatattaca
    38521 tccactttta atactaatgt tctctggtct ttcagtagca ataccagcag ggcttgaagg
    38581 aatttcaaat cctggattcc atggattatc acaggtattg tatgagtttg catcttcagc
    38641 agccaataat ggttcaggat ttgaaggtct aggagataac actatgtttt ggaatataac
    38701 aacaggtgta gttatgttct ttggaagata tgtgtctatt atagtactac tagcaatatc
    38761 aagtttgtta gcatctaaaa aagctgtaaa tgaaagtata ggaacactta gaacggataa
    38821 ttttacattt acaatagttt tagtacttgt tgttttaatt gttggtgctt taacattttt
    38881 cccagcctta gcgcttggac ctatttcgga gcatctagtg ttgtggcact aaaaaattgg
    38941 gagggagtaa agttttatgg aaactaaaaa aacaaagttt attacaaaag atattttcaa
    39001 aacttccatt atagaagctt ttaaaaaatt aaatcctaaa tacatgatga aaaatccagt
    39061 tatgtttgtt gtagaaattg gatttatagt aacattgata cttacaatag tacctagttt
    39121 gtttggtgat gaaggcaata acttaagagc ttataatggg attgtaacaa taatcttatt
    39181 tataacagtc ctttttgcaa attttgctga agctgtagct gaaggaagag gtaaagctca
    39241 agcagataca cttaagaaaa ctagaaaaga tacagtagca agggttatag atacgttagg
    39301 aaatgaaaaa atgataagtg caagtgagct aaaacttggt gatattgttt tagtaaatgc
    39361 tggagaagtt attccaaatg atggagaagt tatagagggt atagcttctg tggatgagtc
    39421 agcaataact ggagaatctg cacctgtcat gagagaatca ggtggagatt tcgcatctgt
    39481 tacaggtggt actacagttg taagtgactg gttaaagata agaataacaa caaaaccagg
    39541 agaatcgttc ttagataaaa tgatagctct tgtagaaggt gcttcaagac aaaaaacgcc
    39601 aaatgagatt gctttaaata ctttattagt agggttaaca cttatattta tggttgtttt
    39661 aatatctctt tatccaatgg caaactatgc aggtgtaaag attccaattt ctactatgat
    39721 agctcttttg gtatgtttaa taccaactac tataggtgga ttgttgtcag ctattggtat
    39781 tgcaggtatg gatagagtaa ctagatttaa tgtaattgca atgtcaggaa aagcagtaga
    39841 agcttgtgga gacgttgata ctatgatttt ggataagaca ggtacgatta cttatgggaa
    39901 tagacttgca tcagaattta tagcagttgg aaatgcagat tcaaaagatt taataaatta
    39961 ttctgttatg tgttcattaa aagatgatac accagaagga aaatctatag ttgatttagg
    40021 tatgaaatta ggttcaacag gtaagacaaa agaggcagag gaagctgagt ttgtagaatt
    40081 ttctgcacaa actagaatga gtggtattaa cttagctgat ggtagagcta taagaaaagg
    40141 tgcttatgat agcataataa aaagagtaaa agaatctggt ggtacagtgc cagaagattt
    40201 agaagaaaat gtaaatcgag tagctaaact aggaggaaca cctttagtag tgtgtgtaga
    40261 taatgagata tatggaatta tctacttaaa agatacagta aaatcagggc ttgtagaaag
    40321 atttgctaga cttcgagaga taggaataaa gacaataatg tgtacagggg ataatccctt
    40381 gacagcagct acaattgcaa gagaagctgg agtagatgga tttattgcag aatgtaaacc
    40441 agaagataaa atagatgcaa taaaaaaaga acaattagag ggtaaaatag ttgcaatgac
    40501 aggtgatgga acaaatgatg ctcctgcact tgctcaggca gatgtaggtc ttgcaatgaa
    40561 tagtggtaca acatctgcaa aagaagcagc aaatatggta gatttagatt cagacccaac
    40621 taagatattg gaagttgttg aaataggaaa acagctttta ataactcgtg gggctttgac
    40681 aacatttagt atagcaaatg atgttgcaaa atattttgcg attataccag ctatatttac
    40741 tcttgcaata cctcaaatgg agatattaaa tgtaatgaaa ctttctacgt catttagtgc
    40801 gatattatca gctttaatat ttaatgctat aattatccca tgtttaatac ctcttgcaat
    40861 gaagggagta aaatatagac ctatgaaatc acaagctatg ttaatgagaa atatgcttat
    40921 atttggtctt ggtggagtta tagtaccatt tttaggaata aaattaattg atttattgat
    40981 tacaccttta gtaatggttc ttaatttagg ttaataaatg gaggatataa aaatgaaaac
    41041 attaaagcca gcattacttg taagtattgt attgttagtt gtttgtggac ttgtatatcc
    41101 acttgttttg acaggtgtaa gtcaagttgc atttaaagat aaagccaatg gaagtatgat
    41161 agaagtaaat ggtgtaaaag taggttcaga acttattggt caaagcttta cagatgcgag
    41221 attctttaag ggaagagtat catcagtaaa ctataatact tatacaaaag aagatttagt
    41281 accagataaa gatggaaata catcttatgg aggtgtatca tcagggtctt ttaattatgg
    41341 agctacaaat ccagaacttc atgatagagt acaaaaagat atagaaaagt ttttaaagga
    41401 taatccaact gtcaaaagag aggatatacc aacagattta cttacagcat caggttcagg
    41461 acttgaccct aatataagtc cagcctctgc aaaaattcag ataccagcta tagcaaaagc
    41521 gtctggaata tcagaaagta aattacaaaa aattgtagat gataatactt ccaaaaaact
    41581 ttttggagtt ttaggtgaag atagagttaa tgtattaaaa gttaatgtag aggtcgcaaa
    41641 aatattaggt cttatatagt attttgtgga attttagcaa caaaatatta cgcaagattt
    41701 aatttaaagg ttaaatttta aagagtgaaa atcgataatg attttcactt ttttatttga
    41761 atattactaa tatttggaat aaagtgattg taagtataaa atccaaaaaa aggttgcatt
    41821 atcaaaatga taatactata atatatttat cgttatcaat ttgataatag atttggacat
    41881 ataggaggaa atagtatgtt atataataac gcattagagt tgataggaaa cacacctgta
    41941 gtaaggctga ataatttagg ctgtactaat gggtgttcta acatatatgt aaaattagaa
    42001 aaagtaaatc cagcaggaag tataaaagat agagctgttt atgaaatgtt agagggctta
    42061 gagcaaagag gagaattaaa aaagggagat gtactagttg aagcaactag tggaaatact
    42121 ggtattgcat tatctatgat aggaaaatta aaaggatatg aagtagtaat cgtaatgccc
    42181 gaaacaatga gcgttgagag aagagattta atgaaggctt atggagcaaa tttggtgctt
    42241 acagatggta aagctggaat ggcaggttcc atagaaaaag caaatgaact tttaaaagaa
    42301 aatccaaact ataaaagcct aaaacaattt gaaaataaag ataatccaaa tgcacattat
    42361 aaaacaacag cagttgaaat tatgaatgat gtcaaagatt tagatatatt cgtatgtgga
    42421 gttggaacag gtggtacttt aataggaact gcaagatatt taaaagaaca aaatccaaat
    42481 ataagagtaa ttgcacttga accaaaaaaa tctccagcta tatcagaaaa caagactgga
    42541 cctcataaaa tacaaggaat aggtgctaac tttgttcctg aaaattatga ttctagtgta
    42601 gttgatgaag taatattagt agatgatgaa gatgcatttg atacagttaa attacttgca
    42661 gcaaaagagg gaattttagt agggatatct tctggagcaa atatatttgg tgctatagag
    42721 atggcaaaaa agtatccaga taaaaagata gttacagtag ctccagatgg agttgataag
    42781 tatatgtcta tgggaatatt ttaataattt tatttgaggg agtgaatact ttgtttaaga
    42841 aaataaataa agatattgaa tatataatga agaatgatcc agcagctagg tctaagatag
    42901 aagttttttt actttatcca tcagttcatg caatgataat gcataggatg gctcatgctc
    42961 tttataagaa gaagaagctt tttacagcaa gattaatatc ccagatatcc agatttatga
    43021 ctggcataga aatacatcct ggtgctaaaa tgggggaagg tatattgata gaccatggaa
    43081 tgggtgttgt aattggagag acagctgaag tagggaatag ggtgacaata tatcaaggag
    43141 ctactttagg agctacagga aaagatactg gaaagagaca tccaactgtt ggtgatgatg
    43201 tattaattgg tgctggaaca aaaatactag gacctttaaa tataggttca aattcaaaaa
    43261 ttggtgcaaa ttctgttgtc gttaaggatg tacctaatgg tgcaacagtt gttggtatac
    43321 cagcaaaaat agtaaaaata agaaacctag aacctgtaaa aaagaataaa aaagaagtgt
    43381 cctatgagta tgatgaatta gataatgtat attatatata ggatttatat aactataaaa
    43441 taaactttat aaaaattatt ttaaaaggta tttttttcaa atttgaggac ataataaaaa
    43501 agaactgaat gttacagttt tggttcatca atctttgata aacaagattt taaatttgta
    43561 gcgtctagga ggattttatt atgaaagcaa atgttaatca agatacttgt ataggttgtg
    43621 gattatgtcc atcaatatgt ccagaagtat ttgatatgaa ggatgatggg aaatcacatg
    43681 ttatagttga tgaagtacca agtgatgctg aagaatcagc agcagaagct agagaaagct
    43741 gtccagttga tgcaatagat gtgcattaat aaaataaatg cctataaaaa ttgaattttc
    43801 aatttttata ggcatttgtc ttttattcag ttagtatttc aacacctgtt tctgtaacta
    43861 aaaccataca ttcccattga gcagataatt taccatcttt tgtataaata gtccaattat
    43921 tttcttcatc aacaaaaatt tcgtgagttc cttcatttac cataggttct attgtgaata
    43981 tcatcccagg aaccaatagc atctcagtcc cttttttaga tacataactt acaaaagggt
    44041 cttcatgaaa gcttaatcca actccatgac cacctatttc tctaacaaca gagtatccat
    44101 ttttctttgc ataagagttg atagcatcac ctatatctcc taaaaatccc catggtttgg
    44161 caacttcaag acctttttgg acacattcct ttgtaacttg aactaatttt tttgcatttt
    44221 cattaacatt acctatcata aacattcttg aagcatctga atagtatcca ttaaaaatag
    44281 tagaaacatc tacatttata atatctccat cttttaagat aatgtcttta cttggaatac
    44341 catgacaaac ttcattattt actgatgtac atacactttt aggaaatcca ttatagtcta
    44401 gtggagcagg gatagctcca ttttgtagtg taaagtcatg aaccaactta tttatatctt
    44461 ctgtagacat tccaatttcg attttatctg aaatataatc caaaattttt gtgtttatta
    44521 cagcactttt ttttatttcc tcaatttgac tttcattttt tataatatca tgagttggta
    44581 caatatgacc taacttagca aatgaatcta ttttattatc catatccata tgacattttt
    44641 tatatttttt agtacttcca caccaacagc tatcatttct atttaacttc ataacttttc
    44701 atccttcttt tattatttat ttaaattatg ggttacataa actattttaa aacataacca
    44761 ttataaatat tataacataa atactaagaa atcctttata gatagtgatt tattcaaagt
    44821 ataaattatt atatggttat tgttgatgaa aagaggatag ttaaatgtat tattggatat
    44881 tagcgcactt aatagtttag ttcaagtttt tcaagaaaat catatgttag aggacaattt
    44941 tcctttaaag tattttttga atcttcatca agataataat gtacaaatga ttctgcaaaa
    45001 aattcttcta taaacttgct tatatatgca tgtgcaactt gcttagggtc ataaagctta
    45061 tatctttcct gagcaaatac ttccataaat tcttcagaat ttgatttttt atttaaaaca
    45121 tttttatcta tagcatgtgc agtttcatgt aattctaaat tttttgatga atgtacattt
    45181 gcatagctag ggttactata acctatttta gccactatag aattatttct atatatacca
    45241 ggtacatctt tccaagtctt gcctgatctt acccaacatg gaggtaattg atattttaaa
    45301 tcactaaatt ctggttcatc agttagattt gaatttatta attttatttt tatatccttt
    45361 tctttaagtg tctgtaaata tttaatattt atttttgcaa gtctgttttt catattttca
    45421 acttcatctt tatcataatt caagtcatca ggaagtataa gcatttgttc aattatatct
    45481 atactactgt cttttatatt ttcagattct tcatcaaact tatattgaag agtattattt
    45541 tcatagatag ttatatttgt attattttct atagaataag agtgaatatc ctctaaatct
    45601 aaaatattca tttgttgtaa aatatgtttt gtttcgccac tccatactac taaagaaagt
    45661 gaattaaaat taattagtgg attttctttt gcttttatat cattgcttat atctacatca
    45721 aatacatatt caattgagtc ttctatgtta agaccatctg tattcttttt aatatataaa
    45781 tacgcttgtt ttaagtctat ataaatctca tctaaagatt cgtcataaaa ttctgataaa
    45841 ttattagtaa agagttcttc acttaaaagt tcatttttat cagttatact ttcaattaaa
    45901 atattaattt cttttatcat atcgtaaata cttatttttc tatttgcttt ttcactagat
    45961 acattgcatg gcgcaagagc aatgcaataa aaatcttttt cttttttata aaaggcttta
    46021 agtgattttg ataaaagtgt aaagtctaca cctatctgta agttgacctt aagtgagtca
    46081 ttttgtaata attcagtatt atttctttta gctgtattta aattgttttc cataatcatt
    46141 gccccctttt tattagggta tcataatttt tttatatgtg tcaatttgtt tttttgaaaa
    46201 ttataatttt tttgaaaatt ttatttttta taatttgtga attttttatt atagaaataa
    46261 atatgttatt attatgtcaa aatttagcat tagattcatg tagagtatgt tttatacata
    46321 tattataatt aattctttga agattgcata ttcaaaatac acaccatttt ttttaattct
    46381 ataatataat atttatatat ttaatgttag gatgtgattt aaggtgtatt ttacatatat
    46441 tataagatgt aaagatgatt ctctatacac tggatatacc agtaatatag ttagaagaat
    46501 gaatgaacat aaattaggaa ttaattcaaa atacacaaga gcaaagggat ttgaaaaact
    46561 agaagtttat tttgtgacca acacaaaaag caatgctatg aagttagaat actatataaa
    46621 aaaattaact agaaataaaa aattgtctat aataaaaaac ccaagtatac ttataaattt
    46681 aattgataat aaagaagatt atattattgg taaggaaata gaacaattaa cataaatatt
    46741 tatagtaaca ttataatggg tgcatatggt gtaaaaactt gacaataaat actattgtaa
    46801 tataatgaag ttataaatat agtaaattaa aatagctagg ggagccaaga gctgagaggt
    46861 ataccgaccc ttacacctga tctggataat accagcgtag gaaagcttag taaaaattca
    46921 agtatgtatt atttgtgata tatgctattt ctaagctata cctattgtag gtatagcttt
    46981 tttaattaag ttatttaaat taaactttag gaggcaacaa tgagtaatta taaaatacca
    47041 acacttacaa tagccggctc tgattcatct ggaggagctg ggattcaggc tgatttgaaa
    47101 acgtttagtg caattggtac ttatggtatg agtgtaataa cagctataac agcacagaat
    47161 actaaaggag tttttgctgt agaggattta aataagaaaa taataaaaaa acaaatagag
    47221 gcagtctttg aagatatacc tccaagagct gtaaaaatag gaatggtttc aagtcctgag
    47281 attatacttg aaatagttga gaatctaaag aaatacaatc caaaatattt ggttgtggac
    47341 ccagtaatga tatctaaaag tggatattat ctattaaagc cagaggcaaa agaaaactta
    47401 attaagtatt tgataccact tgcatacata attacaccaa atataccaga ggcagaagaa
    47461 ataacaggaa taaaaataca taatgtagat gatatgaaga gagtaggaga agaaatacta
    47521 caacttggac ctaaatttgt acttatgaaa ggaggtcatt tagatggaga agcagtggat
    47581 attttagttg gtaaaaatat atttaaagtt tataaaagtg aaagaattga taaaaaaaat
    47641 acacatggaa cagggtgcac attatcttct gcaataacat cttatctagc attaggatat
    47701 gaaataacag aagcagtaaa tctaagtaaa atctatataa cagaagcaat aaaaagaagt
    47761 tttgatatag gtcatggagt aggaccagta catcattttt ataaatttga gtaataaaaa
    47821 aatattggag gtagaatatg tataatttaa ttaaagatgt aaaaaaatta aatccattgg
    47881 taattcatta tactaataat gtaactataa atgattgtgc taatgtaact ttagctgtag
    47941 gagcaagtcc tcttatgagt ttttcatatg aggaagtaga agaaatggta agtgtagcaa
    48001 actctgtagt aataaatata ggaactatga actcaaatat gttggattta ttcctattag
    48061 ctggaaaagc agcaaataag tacaataaac ctgttgtttt ggacccagtt ggagtatttg
    48121 caagcaaagc gagagcagaa cttacaagta gacttttaaa tgaagtcaaa tttagtgttg
    48181 ttaaagggaa tgtatcagaa ataaagttta taggtggctt taatgtaagg ggtaaaggtg
    48241 tagattcatt tgatgaagaa gaagattcta ctgagataat aagaaagatt gctgaaaagc
    48301 ttgaatgtgt agttgtagca actggaaaaa tagatataat aactaatggt aaaggtacat
    48361 ataaaataaa caatggaact gataaattaa aaggaattac aggaactgga tgtatgactg
    48421 ctagcttgat tgcaagtttt atggctgtta ctgagaatat attagaagca gcaacaatgg
    48481 gtgttcttac aatgtcttta agtggggaat tagctaattt aaataatcca ccaataggta
    48541 catttaaaga aaatcttatg aatgctattt atcaaatgga tatagatgct ttaagtaaaa
    48601 attctaatat tgaattttta aattaataaa gagtaaggga gacttaaaat gattgataaa
    48661 gaatctttaa aaaaatgttt aaagttgtat ttagtaactg actctgaaat gcttaaaggt
    48721 agagactttt ataaatgtct tgaagatgca atatcttctg gaataacaac tgtacaactt
    48781 agagaaaaaa atgcttctgg aagagagttt ttaagaaaag ctatgaaact tagagaaata
    48841 actaaaagat atggtgttaa gtttataata aatgatagag tggatatagc acttatttgt
    48901 gatgcagatg gagtacatgt aggacagagt gacattgatg ttagagaggt tagaaagctt
    48961 ataggaaaca ataaaattct aggagtatca gcaagaactt tagaagaagc tatttgtgca
    49021 aaaaatgatg gcgcagacta tttaggtgta gggtctatat tcacaacatc tactaaatta
    49081 gatgcaaaat cagcttcgtt tgaaactgtt aaagaaatta aagaaaaagt agacatgcca
    49141 tttgtactaa ttggaggtat aaatttagac aatatagata aacttaaatg tttagaaagt
    49201 gacgggtatg ctataatatc agctatatta aaagctgaag atatctctaa agaagtggaa
    49261 aagtggacat taaaaatata aaattggctc tggtagtgaa aaaaatggaa aaatttgata
    49321 taatctaaga gtaaaattta agcaattata aatgataaac ttttctacat atacttttta
    49381 atcaagatat atgtagattt ggttaaaaag gagctataaa tgattaggat attgttagtg
    49441 tgtgttggag gcatgtcttc taccctattg gtaaataaaa tggagaagga tgcaaaaaaa
    49501 agaaatatag actgtaaaat atgggcagtt ggagaaggag atataaaatc ggagcttgat
    49561 aactttgata ttttacttct tggaccacaa cttagattta tgcttgatga cgtaaagtct
    49621 atagttggag atagagctcc agtatctata atagatatgg taaactatgg cacttgtaat
    49681 ggtcatgctg tgttaaattc ggttttagaa atattaaaat aaataataaa agtggattct
    49741 atataataga gtccattttt attatttgtt attaataggc ttaaaactta gcttaccatt
    49801 tcttttttta atctccacat agcaagtgaa aaacttgaac agcacaatat taaaaatagt
    49861 aggataacag gcattatatc tccaataaga atagtctcac caaatattaa gctacgtaaa
    49921 gcgttaataa catgggtaaa tggattgagc atcactgcta cttttaatcc tccagataaa
    49981 ctttctatag ggaaaagagc tgaacttaaa aagaaaatag gaagtacaat tgcagtcata
    50041 attgtttcat aaactatttc atttggcaat aataaactta tagaataagc tagacttgat
    50101 aaaaagaagg ctgtcataaa tatcagtact atcataagta ggattccacc aatgccagat
    50161 tctatcctta ctgagaagaa tatacttact atacacaaaa tagttacttc tatgaaagaa
    50221 actaagatag cttctaataa ttgaccaagg acgattgaat acctgctgat aggtgcaatt
    50281 aagactctat agaaacttcc actgttcttc attataaagt ttataatacc accactacta
    50341 caagaagaaa acacaaccag tacaatgata cctggtagta taaaagcagt ataattattg
    50401 atatttatat tattcatagt ttggtttgca atagaactat aaagcacaag ccaaagaaaa
    50461 ggttgtaata tagtaactac aaatgagatt ggatttttaa aacgccactt catactacgc
    50521 cataagatat ttaacattcc cattcctcct ttaagctttt tttagaactt gtaagtgcta
    50581 aaaatacatc ttcgagactt ggctctacaa tttctattgc atcaaattct atatcatgtt
    50641 ctaaaagcca tttatttatt aaagtaaaag cagttctact atcgtttaca cttataaaaa
    50701 ttgaattttc tcgtacgctc ataaacttta ctaaaccagt acttttaatt gaatctttat
    50761 atttttttat atcttcagta ttatgaaaag ttatacgtag tatattttgt ctaatgtaac
    50821 tacgtaaact tgatggtgtt ccttgtgcta aatcctttcc attcttcata atacaaatat
    50881 tatcactcaa ttgttcagct tcttcaagat aatgagtagt tagaaatata gttgtgccat
    50941 actcatctct aatttttaat aacatatccc acatagattt tcttgaatcg acatccatac
    51001 caacagttgg ttcatctaaa aataggattt taggagatga taccatattc atagctatgt
    51061 ccaatctacg ttttacaccc cctgaatatg atgaagtagg atattttaag taactagata
    51121 agtcaaactt atcaattaaa gagtctattc tttgttttgc aatctgtggt tctactttat
    51181 acattttact ttgaaatacc atattttcca taagggataa atgttcatca atagagattt
    51241 gctgtgctac acaagcaatt tgagtacgta tccaagaagg attatctact aaatcttttc
    51301 caaacattgt tacatttcca gatgtaggtt tgtaaaaggt agtcaaaata tttataagag
    51361 aagatttacc agcaccatta ggaccaagta aagaaaaaat ttctccagca tttacattaa
    51421 agcttagacc atttaaagct ttcacaccat ttttatattc ttttactaaa ttgtctattt
    51481 caatcgctaa catatattat tcctcctctt taagtggaaa tattatctct gtaatatagt
    51541 tatctgggtt tccttttatc aacatacctg gtcctttaat aaatacttct ctccaaggag
    51601 tttgtatttt tatattattt tcatgaatat atttaaatat catttcatac gtttttggca
    51661 aattactata agagcctgtg tgagtaaaaa ataaagcttt tgttttagga aattcttttg
    51721 tagttatacc catacgattt ggagtttcag cagttggaac acataattcc atttctgcaa
    51781 caccagtttt ttggtcaaca tcatagtagc aaaagaatgg tgcaccgtta gactttcctt
    51841 ttatagcttt aaatacattt ggaaaatact ttgttgcctc agtcataggt ccattataac
    51901 gcatggtagc cactgtgact gattcgatat ttttgatttc aattttgcaa ttcatttgtt
    51961 tacctcctaa tgtgtgttca tattcttact agatttttaa gcattaatac atataagatt
    52021 ttgattagtt gaaaacatat aatcttatct cgtttgcttg ttttcaagtc taacataaca
    52081 taaaatatat atcctctcat ttgatgctcc attttgtaaa gttattattt aagctttact
    52141 ttagattttt tagcaaatta aaataaagca atttataaaa taagtattta caaattaagt
    52201 gtattaatcg tataatacac ttaataggat actagataaa gtctgaaata aaggcgtatt
    52261 tttttaaagt aagtgtacta tcgtattaat acactagaat aggaggaatc atgaattttg
    52321 aattagataa tacaactccc atttatttgc aaatagtaaa gtatataaag agacaaatag
    52381 taacaggaga gttgaaacca ggagagacaa taccatcaag aagagaaatg gcacttaatc
    52441 taaaagtaaa cttaaataca gttcaaagag catataagga gatgggtgat atgaatataa
    52501 taaatacttt taaaaattat caaagcagtg taacagttga tgaaaatata ttaaaaaatt
    52561 taaaattaga gcttataaat gaatcactat ctgtttttat agaagatatg aaagcaataa
    52621 atgttagcaa agaagaagtt ttaaaaataa tagaagataa gtattagttt tttaacaaat
    52681 agaaaactta gtttagaaag tttatttata agcatattgg atgtggagga tagtaaatat
    52741 gatagaaata aagaatgtca gcaaaacata taaaaggatg caagggctta aaataagaaa
    52801 gatagaagct ttaaaaaatg taagttttaa tatagaaaag ggaaaaataa ctgcattact
    52861 tggtataaat ggtgttggta aatcaactat gttaaaagca atagcaggac ttataaacat
    52921 tgatagtgga gaaattcgta tagatggaga aaaaataaat gaaaaggtat acaataaatt
    52981 ggcttttgtt ccagatgtac aatctcactt tagcaataca acaataaaag aaacatttga
    53041 atttatggaa attttttatt caaagtggaa taaagaaaaa tcaaaagaaa tgatggacat
    53101 atttaaattg gatgaagatg aaataattga taacttgtca aaaggaaaca ttgcaagagt
    53161 aaaattaata cttggatttt gccaagaccc agagtacata cttcttgatg aaccatttac
    53221 aggaattgat ttgtttaaga gggaagagtt tataggagta atagcgcaat atatggagga
    53281 aaatcaagcc attattataa ctactcatga gatagtagag atagaaagtt tagttgatga
    53341 ggttgtaatt cttgatgaag ggcagattat aacttcattt aatgcagaag aattaagaga
    53401 aagagaaggt aaatctatat tagataagat gagggaggta tataagaatg aataaacttc
    53461 tatatctaat aaatgtagat ttgagaagaa gcaataagtt ttatatagct tatatatcat
    53521 ttttttcatt aattacactt agtttaaatt tattcgaaat taataaattt aaaaattcta
    53581 aatttattat gaacttagca atagaagatt ttggaggtat attttatgga ctagggatac
    53641 ttagtaatct tggttttatc caaagtatat tgttctttgg gatattggga ctttttatat
    53701 actatatttt tatgtggaaa agagagttct tttcaaataa tcaaagtatt tatacactta
    53761 tgatgttacc tcaaaataag tttaagattt gtatatctaa atcaatagcc ttgataacaa
    53821 tgatatatgg ttttttgata agtcagacag caacattatt tatatgtaaa tatatattta
    53881 atttcatttt tagaaatatg cctataatca atatgaactt tgccaaagat ttatattatt
    53941 taaacttgaa gagtatacca attgatttta tctcttttat ggctatttat gtatttttac
    54001 tacttatagc tatttctatt gtaaattgct gtatattatt tatcttttcc tatataaata
    54061 aattttatgt aattttaatc actttgctga ttatagcagg cttatatttt tcaacaatat
    54121 atataaatat aatcacttta tttagaaaat attataatct tataggattt caatttaaca
    54181 atttagtagc agggttatta agttttttaa ttataatgtt tattctaaat ggaatatctt
    54241 atttgctaat taaaaagaaa gtatctcttt aaggaggggt ggtatgaaat taaaatctaa
    54301 aatatctata tttattatat ttttgacttt agcaatatat tttagtatag gtttattaaa
    54361 taatgaagat aatttaaaga taaaaactat aaaaggtgac tctaatgaat tagaccatat
    54421 aaacataaat tttggatatg ctttgaatgg atatataaat tcatattata caataaatgg
    54481 aagcaacaaa accaaaaaga ctgaattaat aaaactacaa aaggatagta agaacacaaa
    54541 taatttaatt gatgtatcta gtggttcaga tttaattgct catgttggat ttgaaatgaa
    54601 tgaggagtca aaagatataa actttgtgat agatgagaaa aaagaagatt tagaaaaagg
    54661 tgtatttcca tcagaaggta gtgataaaga aattgaatat gaaaaattag atatgaataa
    54721 ttttacaagg acaagatata aagtagataa aagtacagat gattattatg caaagatact
    54781 agcatcaaaa agatataaaa aatacttata tgtatctgta ctttatgcta caagtgattc
    54841 atcagaaaat aacaaagaat attcaagtac tatagatata ataaaaatta atccagaaaa
    54901 taaaaaaatc aagactataa aaagtataaa tttaagtaat tatttttccg aaaaatacca
    54961 aaacatagag aacataggtg taagttatgc tagcaaatat gaggataaga tgtatatttt
    55021 agtaaatgta ctaaatacaa acataggtga taatagtaat tctaaatttc atttacttga
    55081 gtataatctt gaagaaaata aggaaagcat aatggaatta aaaactgata gcaaaatggc
    55141 tttagcaaaa gcaaagttag aagctaataa attatatatg gtaatgtcga atgattatga
    55201 taatagttca attacagagt taaatttact tacctataat atattagata aaagcttaga
    55261 tgaagataaa tatgttttag ataatagaga aaaaagagga attagaagta ttgaaattga
    55321 ttcaaataaa atcgttgcat atacatcaag gctaaactct atattatctt gggatgataa
    55381 atttatgcta tatataataa ataaaaattc taaggaaatt gtatatgaag gagaattaca
    55441 atttaacagt tcaaatagta tgttagaatt tgaaataaag taggtgagtt gatgaaagat
    55501 ttaataagat tattcgaatt tgaattaaaa agaaatataa agaattatat atttataata
    55561 atttgttgtt gttcttttgt aattttaaac atagtaaaaa atttaaatga ctataattac
    55621 ataatagaaa atgcagttaa aagtgaacag ttaacaaaaa tcggaaatac gatagaaacg
    55681 gtaaatgtta caggtttctc aagctttaga aatataatgg gttctacaga atcgtggttt
    55741 atgtttggta taattgcatg tatttgctat gcttttttta tttggtatag agactttaat
    55801 ggtagaagta aatctatata tacacttata atgctaccta aaaatagaat caatatttat
    55861 atatctaaat tattaaatat attatttcta gtgtatagct atacagtagt acttacaata
    55921 tctttgttta ttgcaagtaa gttgttgcct agacatatgc ttggtaatgt cacaaactat
    55981 ggttttgtac aagaaacaat atatgagtta aaaatgttac ttccatatag ctttgaaata
    56041 ctatttgtag agtatatatt tttattgata ggatttgtat ctgtagtatt tacatcaatt
    56101 cttataaata aatctattta taaagtaagc actttaataa gttttctatt tttagttata
    56161 gaaattttag tgtttataat ttctatagaa tttattatac cttacgaata cagtgatgta
    56221 tttactgttc tatattcaag tataaacata tttgtatgta gtcttatatc taataaactt
    56281 ttgaagcaaa agattgactt ttaggaggga tattatgaaa gcaaaaaata taggaaaata
    56341 tacaatatgt tttataatag tatgtttttt aatgattact atgaccttaa tattgagtta
    56401 ttttaataag gataaactat ctgtaaaaac tttaaaaggc aatgtaaaag atatagggag
    56461 agtaagtgta gtatatacac ctaatataag caaattttct tatgaggagg taattataag
    56521 taaaaatgga gtaagaaata atacaaaccc aaaatcaaga attagaaatg attatttaaa
    56581 tattgacaaa aaatacagtg actttgttaa tgataaagga aaagaattta tatataaaga
    56641 tgatttgaat ataggagaag cgtatacctc tgttaagtct gatttagtac aaggaatgaa
    56701 tgctttaaat gattgtataa aggtttcaaa taaaaattta aaaagtgggg tagaagaaaa
    56761 ttttgatatt aatttaaata agtctgattt taaagaaaat agttggaaaa ccttatttaa
    56821 caaaatatat aaaggtaatt tatatatggt aattagtgaa actaatattt caggttctaa
    56881 ag
//
LOCUS       DAELTA010000006        55130 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.006, whole genome shotgun sequence.
ACCESSION   DAELTA010000006 DAELTA010000000
VERSION     DAELTA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.006"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(155..964)
                     /locus_tag="KPG13_000312"
     CDS             complement(155..964)
                     /locus_tag="KPG13_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0984060.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1048..1581)
                     /locus_tag="KPG13_000313"
     CDS             complement(1048..1581)
                     /locus_tag="KPG13_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0984061.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1731..3080)
                     /locus_tag="KPG13_000314"
     CDS             complement(1731..3080)
                     /locus_tag="KPG13_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0984062.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3092..4474)
                     /locus_tag="KPG13_000315"
     CDS             complement(3092..4474)
                     /locus_tag="KPG13_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG0984063.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            4718..5932
                     /locus_tag="KPG13_000316"
     CDS             4718..5932
                     /locus_tag="KPG13_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0984064.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6109..8022
                     /locus_tag="KPG13_000317"
     CDS             6109..8022
                     /locus_tag="KPG13_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984065.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8293..9267
                     /locus_tag="KPG13_000318"
     CDS             8293..9267
                     /locus_tag="KPG13_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0984066.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9288..9563
                     /locus_tag="KPG13_000319"
     CDS             9288..9563
                     /locus_tag="KPG13_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0984067.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9576..10739
                     /locus_tag="KPG13_000320"
     CDS             9576..10739
                     /locus_tag="KPG13_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0984068.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11076..13355
                     /locus_tag="KPG13_000321"
     CDS             11076..13355
                     /locus_tag="KPG13_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0984069.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(13450..14790)
                     /locus_tag="KPG13_000322"
     CDS             complement(13450..14790)
                     /locus_tag="KPG13_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0984070.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(14802..15584)
                     /locus_tag="KPG13_000323"
     CDS             complement(14802..15584)
                     /locus_tag="KPG13_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984071.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15599..16621)
                     /locus_tag="KPG13_000324"
     CDS             complement(15599..16621)
                     /locus_tag="KPG13_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0984072.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16627..17640)
                     /locus_tag="KPG13_000325"
     CDS             complement(16627..17640)
                     /locus_tag="KPG13_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0984073.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(17768..18706)
                     /locus_tag="KPG13_000326"
     CDS             complement(17768..18706)
                     /locus_tag="KPG13_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0984074.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            18976..19440
                     /locus_tag="KPG13_000327"
     CDS             18976..19440
                     /locus_tag="KPG13_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984075.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19552..19869)
                     /locus_tag="KPG13_000328"
     CDS             complement(19552..19869)
                     /locus_tag="KPG13_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0984076.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(19882..20613)
                     /gene="chbG"
                     /locus_tag="KPG13_000329"
     CDS             complement(19882..20613)
                     /gene="chbG"
                     /locus_tag="KPG13_000329"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG0984077.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20639..21949)
                     /locus_tag="KPG13_000330"
     CDS             complement(20639..21949)
                     /locus_tag="KPG13_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0984078.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22046..23359)
                     /locus_tag="KPG13_000331"
     CDS             complement(22046..23359)
                     /locus_tag="KPG13_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0984079.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23381..23692)
                     /locus_tag="KPG13_000332"
     CDS             complement(23381..23692)
                     /locus_tag="KPG13_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984080.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(23892..24596)
                     /locus_tag="KPG13_000333"
     CDS             complement(23892..24596)
                     /locus_tag="KPG13_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984081.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24687..26048)
                     /locus_tag="KPG13_000334"
     CDS             complement(24687..26048)
                     /locus_tag="KPG13_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0984082.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26547..27701
                     /locus_tag="KPG13_000335"
     CDS             26547..27701
                     /locus_tag="KPG13_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0984083.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(27783..29501)
                     /locus_tag="KPG13_000336"
     CDS             complement(27783..29501)
                     /locus_tag="KPG13_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG0984084.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(29506..30495)
                     /locus_tag="KPG13_000337"
     CDS             complement(29506..30495)
                     /locus_tag="KPG13_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0984085.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(30591..31640)
                     /locus_tag="KPG13_000338"
     CDS             complement(30591..31640)
                     /locus_tag="KPG13_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0984086.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(31917..32585)
                     /locus_tag="KPG13_000339"
     CDS             complement(31917..32585)
                     /locus_tag="KPG13_000339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG0984087.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(32591..33964)
                     /locus_tag="KPG13_000340"
     CDS             complement(32591..33964)
                     /locus_tag="KPG13_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG0984088.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(33961..35739)
                     /locus_tag="KPG13_000341"
     CDS             complement(33961..35739)
                     /locus_tag="KPG13_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG0984089.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(35856..36179)
                     /locus_tag="KPG13_000342"
     CDS             complement(35856..36179)
                     /locus_tag="KPG13_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG0984090.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(36166..37143)
                     /locus_tag="KPG13_000343"
     CDS             complement(36166..37143)
                     /locus_tag="KPG13_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG0984091.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(37158..37721)
                     /locus_tag="KPG13_000344"
     CDS             complement(37158..37721)
                     /locus_tag="KPG13_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG0984092.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(37773..38264)
                     /locus_tag="KPG13_000345"
     CDS             complement(37773..38264)
                     /locus_tag="KPG13_000345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG0984093.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(38267..40192)
                     /locus_tag="KPG13_000346"
     CDS             complement(38267..40192)
                     /locus_tag="KPG13_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG0984094.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(40194..40505)
                     /locus_tag="KPG13_000347"
     CDS             complement(40194..40505)
                     /locus_tag="KPG13_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984095.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(41135..41410)
                     /locus_tag="KPG13_000348"
     CDS             complement(41135..41410)
                     /locus_tag="KPG13_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG0984096.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(41525..42934)
                     /locus_tag="KPG13_000349"
     CDS             complement(41525..42934)
                     /locus_tag="KPG13_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG0984097.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(43305..43967)
                     /locus_tag="KPG13_000350"
     CDS             complement(43305..43967)
                     /locus_tag="KPG13_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG0984098.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(44221..46440)
                     /locus_tag="KPG13_000351"
     CDS             complement(44221..46440)
                     /locus_tag="KPG13_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0984099.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(46716..49370)
                     /gene="adhE"
                     /locus_tag="KPG13_000352"
                     /gene_synonym="adhC"
     CDS             complement(46716..49370)
                     /gene="adhE"
                     /locus_tag="KPG13_000352"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0984100.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            49874..50074
                     /locus_tag="KPG13_000353"
     CDS             49874..50074
                     /locus_tag="KPG13_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984101.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(50281..50871)
                     /locus_tag="KPG13_000354"
     CDS             complement(50281..50871)
                     /locus_tag="KPG13_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG0984102.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(50874..51749)
                     /gene="dpsA"
                     /locus_tag="KPG13_000355"
     CDS             complement(50874..51749)
                     /gene="dpsA"
                     /locus_tag="KPG13_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG0984103.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(51825..53216)
                     /locus_tag="KPG13_000356"
     CDS             complement(51825..53216)
                     /locus_tag="KPG13_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG0984104.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(53220..54356)
                     /locus_tag="KPG13_000357"
     CDS             complement(53220..54356)
                     /locus_tag="KPG13_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG0984105.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(54388..54933)
                     /locus_tag="KPG13_000358"
     CDS             complement(54388..54933)
                     /locus_tag="KPG13_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG0984106.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT        18482 a         9482 c         6743 g        20423 t
ORIGIN      
        1 attataacaa attttaggaa tatataataa tagggtctca gaacttagtt acaagttcta
       61 aaaccctatt tgtttagtgt aaaaaacatc ataaaattta gcttaaatct tttcacataa
      121 tattactctt tttctaagtg ataataaaat atatctattc ctctaaaaac tgtaaaaatt
      181 cctgattaaa tagtttaagc tcatcataga atatagcatg accactatac tcaaaagtat
      241 ataaaattga atcttctatt ttttcattca taaaaactgc aaattcatac ggacatattt
      301 catctaattt cccatgaaat ataccagtag gcactcttac acattttaaa tcattaaata
      361 attcttcatc tcttaaagat acagcagttc caatagtgcc aatacctgat gcactaaaac
      421 caatatcatt aaaccatctt ctaaagcttt ctggtggatt tgatgcaaat actttttcgc
      481 caaaatcggt taccatttct ggtctatcag tacatgcttg tgctatcaat ttatttacat
      541 cttcacgtgt cataccatat ggaaattctg gaggtctttg tacaaatgat ggtgcagcag
      601 ctgcagctaa taccaatttt gacactccat atccattaaa taaactcata tacctaagta
      661 cgattgctcc tcccattgaa aaaccaacaa gggtaaagtc ttttaatcca atagcatgta
      721 ccaccttata aatatcgtct gctaattggc tataagtata tcctcctgat gttgcatcag
      781 atttaccaaa acctcttaaa tcaattgaaa ctgttctata tccgagtttt ggcaatatat
      841 ttgtttgata ttcaaatatc ttatgtccta agggccaacc atgtataaaa agtacagttt
      901 ttttagcact cggattcaaa tcatatactg ctatgtttat atcattcaca tatacataat
      961 acatatcaac agcaactccc ttcaagaaaa tgtatgatgg ttattttata tatatgaata
     1021 aaatgatgaa taactaaatt ttcaaattta tttacaatac ttctctattg ccttttcaaa
     1081 taaacttaca tgaagttttt catctaggat tattctattt aatatagctt ttatataagg
     1141 gtcttcaata agacttatat ctttataata attattaata gccacatgct ccaaatgcaa
     1201 atctagtttt aatctgttac aaatagaatt tccataacat acaaaaccac cattccaata
     1261 tgcaccacaa gaacttgtag aacctctata tacaggattt ccacccaata atcttatagt
     1321 ctttgctaat atctccatat gtaacatttc tgttatagat atatttatcc acatttcact
     1381 taactctcta tgcgtttcat ctaaatcaaa attatggtat agatattgat ttacactcgt
     1441 aaattcactt gatactccag catagtcatc catcaataat tctgcataat acttatttgg
     1501 acctaaaact tttatctctg gatatggctc atcacttgaa tatccatttc gcttgtgcat
     1561 gtcataatgt gcaccactca taatatctcc tcctttaaat ttaatgtatg atattaattg
     1621 tatgcatcac aactttttca tataacttag ataataaaat ctacttaaat aataaaaagc
     1681 catattaatt aaaattaaca tgacctttta tccacaaaat tactttttaa ttattcatct
     1741 tttccagcta ctgatagctc tttaataaca actgaaggtg aacctatact actcattgga
     1801 aattctaaat cttctcctat agcttcaata tctttcaaca ggtcaaaata gtttcctgct
     1861 actgtaattt gctccactgg aaatactttt tttccttctt tgatataaaa gcccttggca
     1921 gctaaagaaa agtctcctgt tactgaattt gcacctgaat gaagtcctgc aaagctagta
     1981 accatcaatc cttcatctat atcctttata atttcatcta aagatttatc tcctttctct
     2041 atataaaaat tagttggata tatgcttata ggtgatgaat atgaagattt tattccattt
     2101 cctgtagtct ttacacctgc cttattagcc gtttttaagt tatgaagtaa agttataagc
     2161 tttccattta aaacaacttc ttttttaaat gtagctactc cttcatcatc aaaaggagta
     2221 gatgccatac cattttctaa aagtgggtca tcaactattg taactatagg agatgctatc
     2281 atgtctcctt ctctattttt caataaagat aatccttttt gagctgaatc tgcattaaat
     2341 atcccagaaa atgtgctaag caaagatacc atagcttcat taagcaatat agttttatat
     2401 ttacctgatg taatactctt accacctact ttagataaag cattatcaac accaagtttt
     2461 gctatctcac aaggttttat ttcttcaata gaatttgcta tattatatcc tattccatca
     2521 tatttttgtc cattgtcttc tatgatagga actacaaacc ctataagcat attagtttta
     2581 ttgcttaaat taagaccttt agtattagat ataccattac ttgatacaga ataagatata
     2641 gtacatttag ataaatttac caccttatct gaataagctt tagtctctct ttccatttct
     2701 aaagctaaat caatcaactt atctgcctct aaattttcta gctctttaga ataagtcttt
     2761 acatcattat agtgcttatc tccttcatat ataaattgta catcttcatt ttcaatagtg
     2821 tttactccat cttttacatt ttttatcaac atatcaacag ctttatcatc taaaatttct
     2881 gtataagaat aacctatctt tccatttacc ttacctctaa aagataaacc aaaagattta
     2941 tcaagattgt atttctcaac ttctccttca tatatattta tacttaaatt ttctcctgta
     3001 taataatata tttcacactc ttcaaaacct tcacttagag cctttttaaa taatatatct
     3061 ttaaaatttt taagttccac aattatccct cctatcttcc acctacagta atttctttta
     3121 cccttatcat aggttggcct acattagttg gtatacttcc tgatgatgag ccacacatcc
     3181 cctgagcttg ttttacgtta ttcccaacca tatctatatt cataagtata tcgctacctt
     3241 ttccaattaa actagcacct ctaactggct cttgtatttc tccatttttt attaagtaac
     3301 cttctgaaac tgcaaaatta aactctcctg ttacaggatt tacagaacca ccacccattt
     3361 ttttagcata aagtccatct ggtattgact ttataatatc ttctggtcta tcttctccag
     3421 cagcaatata agtgttagtc atccttgaag ttggtgcaaa tttgtagttt tgtctacgag
     3481 aacttcctgt aggattcatt cccattcgtc taccattaag tttatctatc atataagact
     3541 tcaaaattcc actttcaatc agtatattct tttgagttgg atttccctca tcatctatat
     3601 tcatagaacc ccagtagttt ggtattgtac catcatctat agctgtaatc tttgtggaag
     3661 caatctgttg acctaattta tctgcaaata cagagttccc ctttgcaact gatgtagctt
     3721 ccaaagaatg tccacaagct tcatgaaata ttactccacc aaatccatta tctattgcta
     3781 ccatcatatt acctgcagga caatttttag catgcaacat agtatgcgct actctagctg
     3841 attcttttgc ataatattct gggtctattt cttcaaatat ttcaaaccct ttacaacctc
     3901 caggaccttc aaatccagtt tgattttcat tacccttaga tgcaattgaa cttatcccta
     3961 gtctagttct tattcttcta tcctcaacat atataccttc agtgtttgca attaatattt
     4021 tttggtcttt atccaaataa ctgacactta cttgagatat atcattacta aattcttttg
     4081 cactcttata tgcaaccttc ataacactta ctttatcttt atttcctatt gaatttggat
     4141 aaatttttat attatgtatg ttatttattt taatactgtc atttaatatt acactttttc
     4201 catcttccaa tttacctaaa gcaacagcag ctttgtatgc tacatccaac agactattta
     4261 atgaattgtc acttgtataa gcatatatac ttttcaatcc tttaaatatt cttattccta
     4321 taccataatt tcttccacct attgcatttt ctactttatt atctattaaa ctaatagaat
     4381 tatttatagt gtcttcttca aaaatctcag caaaatctcc tccagtaatt agacattttt
     4441 ctaatacctt agaagctata ttttttgaaa gcataaattt acctcctact ttaaattttt
     4501 aattaacttt tattcaatct ttttacttgt taacaaacat tatatactat attacttaaa
     4561 ataaggtaat tatgaatata ttatacatat gttttatact aaaatgttat cacattttat
     4621 ctatacatac ccatataact agataattaa aatgtataat attatttgct atagaaaata
     4681 atacactcat acataataaa ttttaggagt gaagtatatg aatcaagaat atgtaaaagg
     4741 aaatcctatt tttactaata ttaataaagt tccaagacaa tttccatacc ttactgatga
     4801 tattgatact gatgttataa ttgttggtgg tggcgtaact ggatgtattt gtgcttacta
     4861 tctagctaaa aacaatataa agtctgtcat tcttgaaaaa ggaaggattg cacatggaag
     4921 cactagcgtg acaacttctc tattacagta tgaattagat gacaatttga tagatttaac
     4981 agaggttatg actctaaaag atgctctaaa agcatacaac ctttgtactt cagcacttga
     5041 agaattagat acatttatag agctatatgg aaataagtgt gattatgcca aaagagatac
     5101 tctactttat actgccaaca aacttgaagt taaggctatc aaagaagaat ataacttaag
     5161 aaaagaaaat ggatttgatg ttgaatatat tgatgaatcc acaaaccctt ttagctttga
     5221 tttaaaatca ggattgatag caaagaatgg aggacgagaa ttagacccat ataaatatag
     5281 tcaccatttg attgatgtaa gtttaaaaaa tggtcttcaa gtatatgaaa atacagaagt
     5341 aaaaaaagta gatttatcaa atgataaggt tactgctgaa gtatcgtatg gatacaaagt
     5401 tcatggtaaa aaattaattg tagctacagg gtacaataca agtttattta caaaaagaaa
     5461 ttttgcaact aagtcaaata cttttaatat agcaacgaaa ccacttaaaa acatagcttc
     5521 ttggaaaaac aacattttaa ttagagataa ctgtgaccca tataattatc taagaactac
     5581 taaagacaat cgattaatta taggtggtga agatgtaagt tttgatgata tagaaaatga
     5641 aactttagcc aatgaaaagt atgatatttt agaacaaaga ttgaaaagta tgtttaagga
     5701 tataaaagac attgaagttg aatataaata ttgtggttgt tttgcttcaa ctttagataa
     5761 tttaggtttt attggtccag ataataaaaa taacaatcta tggtattgct taggttatgg
     5821 agcaaatggt atactctttg caatattagg aggtataatg ttaagtgaat tatatcttgg
     5881 caaacaaaat aaaaatatga aattatttaa agtagataga tttgataaat agatattata
     5941 tattaaaaga ctgtaaaaag atgaaacttt ataattctaa aagttaactt tgatacagtc
     6001 ttttataatg ttccttttaa aaatacttta tttataatta aatataaagt aaaatgttaa
     6061 aatataacta ataaaattta gtcataagat aatatttaag tttatttgat gaaatatgga
     6121 ggaattatta tgaaggaaat actcgttatt gctcccacta aaggaaccta tgaaaaatca
     6181 attcacatag ttaaaaaaaa caaatataca aatattgatg ttgtatttgg caatctaaaa
     6241 gaaggtatac ctcttgctga aaaaagtatt aatcatggta ctagaattat cataagccga
     6301 ggtggaactt acaatatgtt aaaagctact tacaatatac ctatagttga aattaaagta
     6361 gatgcttatg atattataaa aagctacaag gaggtaaaaa attccaacga accatttgga
     6421 ataattggat ttaataatgt catctacggt ttcgatatta tagaagaaat attgaataag
     6481 aaaataacta tgattgaaat tgaaaaagaa gaagaaattt atgatgctat agaaaagtac
     6541 agaaagaaag gtataaacac atatatagga gataccactg ttgcacatat tgttaagaga
     6601 cttaattgta agggaatatt aatcgaatct agagaagaaa acatacttag agccattcaa
     6661 caagcagaac aaatactcga agcaaccaag gatgaacaaa aaagaagatt acagatagaa
     6721 gctatgacag attttgtaca tgatggaata ataacagttg ataaaaattt taaaataaca
     6781 ttatttaata agagtgctga aaaaatattt ggtataaaaa aagaaaatgc actttatcat
     6841 aatgttgtag atgttatccc aaacacagtt cttccacaag tagttgagac tatggaacct
     6901 caaattggtg aaatacaaaa tatagggagt acaaaaatta ttacaaatag agttccaata
     6961 attgttgatg gtcagattca aggggctgtt tcaacatttc aagatagcaa ggagataagt
     7021 tcttttgaac atacaataag aaggagctta ttaagcaaag gtctatctgc taaatacact
     7081 tttgatgata taattcatgc tagtgaaaag acaaaagatt gtatagaact tgcaaaaaaa
     7141 tatgcttgtt atgatactcc tatgattatt actggagaat ctggagttgg taaagaacta
     7201 ttttgtcaaa gtatacataa ctatagtaag cgtaaaaatg caccttttgt tgctgtaaat
     7261 tgtgctgcca tacctccatc tctaatagag agtgagtttt ttggttatga ggaaggttca
     7321 tttacaggtg ctagaaaaaa aggtaaagca ggtgtattcg aactagcaca tgaaggcaca
     7381 atcttcctag atgaaataag tgaaatccct cttgaacttc aaggtcgatt attgagggta
     7441 ttgcaggaaa aacaagttat gagaattggt ggtgataaag ttattcctat agatgtaaaa
     7501 attatctgtg cttcaaataa agacttaaaa aatatgatgc gcaatggttt atttagacaa
     7561 gacctttatt ttagaataaa tatactaaca ttatatcttc cttcattaaa agaaagaaaa
     7621 gaagatatta taaaattatc agaatttttt atacataaat attcaagcaa atacaataaa
     7681 acgcctttaa ttatcactcc aaatattaaa aaggcacttc ttgaacgaga atatgaaggt
     7741 aatataaggg aattggaagg tatgatggaa agggctgtta ttgtggaatc atttgactcg
     7801 attttattag aaaagcttag caatgaatat acaaataata tagttgataa agataatagt
     7861 gattttgaat gcagtaattt agatttaaaa actttagaaa aaaaatatat ctataaaatt
     7921 tataacgaaa ataatagaaa tactacaaaa acctgtgaaa ttctaaagat aactcgctcc
     7981 actctatggc gtaagttaaa agaaatagaa gatgatgttt aaaaataata catatctata
     8041 tatatcaaaa cgttttatgt ttaattttaa tacataagac gtttatttgt tttcaataaa
     8101 aaatacaatt atatgctaaa aagaatcttt gtaagtgaaa ttctcgtcaa tcatactttt
     8161 aattataagt aatctacagc atttcaatcg attattaaaa ataatcatga taactaaaga
     8221 tacaaactct atatattggc atagtaattg cttttataat acctatataa aaatgaaagg
     8281 gagtgatact agatgcacat cttaaaatca gtgaaaaaga taccaggagg acttatggtc
     8341 tgtcccttgt tattgggagc actatttaat acagtatgtc cacaagctct tgaaattgga
     8401 ggattcacaa catccttatt taaaactggt gcaatgagta tacttgctct attttgtcta
     8461 tgcaatggag ctcaaatcaa tatcagacaa gctggtaaac cattgactaa aggtatagta
     8521 cttacagcaa caaaatttat aattggtgca gtattaggta ttattgttag taaatttatt
     8581 ggacctaaag gtataattgg tattacacct cttgccatag ttgctaccat gactaattca
     8641 aatggaggtc tatttgctgc tcttgcaggt gaatatggag actccacaga tgttggagca
     8701 atatctatct tatctctaaa tgatggtcct ttttttacaa tgcttgcttt tggagtaact
     8761 ggacttgcaa atgtacctat agtagcacta tttgcagcac ttataccaat actacttgga
     8821 tttatacttg gtaaccttga tgaagatata cgagaatttc ttgcacctgg tacaacatta
     8881 ttaattcctt tcttcgcttt tcctttagga gcagcattaa actttaatca aattataact
     8941 gctggactac ctggagttgt acttggttta ggagttacta taataacagg tatgggtggg
     9001 tactttgtaa tgaaacttat acgtgcagaa catccagcag taggtgcagc tacaggtaca
     9061 acagccggaa atgcagctgg tactcctgaa gctttagcag ctatagaccc tactctagct
     9121 acaatagcag ctgtttcaac agtacaagtt gctgctgcta ttattgttac agcaatatgt
     9181 tgtccaatgt tagtttccta tctagataaa agagaaaaaa agaaaaaatc tatttttaaa
     9241 acaaataaaa atattgaagt aaattaatac ttaaggaggt aaaattaatg aatgcaatta
     9301 ttcatgcaaa tgaaaaagat aatctagtta catgtgtcag acctttaatt aaaggtgaaa
     9361 aagtttcaat tgatggaaag tcttacatag taaatgataa tatacctgtc tttcataaga
     9421 tggctacaga agatgtaaaa aaaggtgatg ttgtatacaa atacggtgaa attataggaa
     9481 ttgcaacagt tgacataaaa actggtgact atgttcatgt acataacatt gaaagtacac
     9541 gtggtcgtgg agataaaaaa taggaggaag ttaaaatgaa attaatgggt tatttaagag
     9601 aaaatggtca gtttggtatt cgtaatcatg ttttaattat tccaacatct gtatgttcaa
     9661 gtgagacagc tacaagaata gcatcacttg tgcctggagc aatagcaatc cctcatcaac
     9721 atggatgttg tcaaattggt tctgatatag agcttacagc aaagacatta ataggatttg
     9781 gaaaaaatcc taatgttgcc gcagttttag tagttggtct tggatgtgat ggtattcaag
     9841 caaaagaatt agcatctgaa atagctacaa caggaaaaaa ggtaaattac gttgttatac
     9901 aagaatgtgg tggaacatta aaaacagttt ctaagggagc tgaaatagta agtaaaatgg
     9961 ctcgtgaagt atcaaaagaa gtaagagttg aatttggtat gagtgaaatt acattagcac
    10021 ttgaatgtgg tggttctgac ccaacaagtg gaatagcttc aaacccttca ataggcgttg
    10081 catcaaacct tcttgtagat gaaggtggaa gtagcatact aagtgaaact acagaagtaa
    10141 ttggtgcaga acatttgttg gctacccgtt ttgaagatga agaaatgaaa gataagtttc
    10201 ttaaatttgt aagtgatgta gaaaaaagag caattgctat gggagaagat ttacgtagtg
    10261 gtcaacctac tccaggcaat aaagctggtg gactttcaac aattgaagaa aagtcacttg
    10321 ggtgtatgta taaggcaggc aataagccat tcaaaggtgc tcttgaatat gctgatattg
    10381 tacctcctga taaaaaagga ttatatttta tggatactcc aggtcaagat atagattcta
    10441 tcactggtat ggtagcaggt ggtgcacaaa ttgtaatctt ttcaactggt agaggtactc
    10501 caacaggaag ccctatttca ccagttatta aaatcactgg gaatagtgat acatacaaca
    10561 aaatgccaga caatatcgat ataaatgcag gtagaattat aacagatggt gctaaaatag
    10621 cagatatagg tcaagaaata tttaatgaaa taatagaagt ctgtaatggt aaacatacca
    10681 aagctgaaag tcttggtcat agagaatttg gcatttacag aatttcatct acattttaga
    10741 ttgtaacaaa atatctttta taaatataat acttatgtag atacataagc taaaagttct
    10801 ttctaatact ataaaaattt tctatactga aaaggaactg tatcaaaatt attttgaaaa
    10861 cagttccttt tttaacctct tttactcaaa atattataaa ataagattta aacaataaac
    10921 gtatcttgat taatctctaa gaagacataa ataaagctaa atgtaaagtg ggataaataa
    10981 aatttaatat ctttgtatga aaagatattt ggtataatat gtctgagaga attcttattt
    11041 gtaaacaaat aaaggatgga ggatgaaaca aaagaatgaa ctttgaaaac acaaaaaata
    11101 aaaaaataat agctacttta ctattgacac tccttttaat cgtgtcatta acatgtgtca
    11161 cttttattat gaatctaaat aaagtaacac ataatgaaac agaacgatat cttgtagaag
    11221 tttcacaaag tatttccact acaattaatg tgaaatgtaa aagtatattt aggacactat
    11281 attctatggg agatacttat tttgagatta atgatagtga aaaagaagtc aatagatact
    11341 tatcaaaaaa attaaaatta tttgaatttg attggttagg tattatagat tctaatggaa
    11401 attttgtaag tgcccttaaa gatacaggta atgttaaagg acaagacttt tatagaaaag
    11461 cccttcaagg caaagaaaca attactcctg ttaaatcaca atctggtaca tatggaattt
    11521 tatattctgt accactatac aatggtgata aaattgttgg agctgttgct gcttggaata
    11581 ctattgatac tatgagaaat ttattaagtg tagaaagctt tcatggtgaa ggattttccc
    11641 aaattatcga ccaagatgga aattttattg taagttcagc aaacaaaaat gctccaaaaa
    11701 atgtggtaaa tttttttgac atgattaaac accaaggaaa acaggttgat aaaaataaac
    11761 ttgaagaaat gaaaataaac ttaaaaaaga acaaaactgg ttctatgtat tatacattaa
    11821 atgatggtat ttctaaggca atgcattatc ttccattaca agaagaaaac ttatacctcc
    11881 tttcaattgt tccaacaaaa acagcaagtg cacagtttga tttacttcta aaaaaagcaa
    11941 tcttcatcaa tattttaatt gttatactat ttttattgct tattatattg attatgttta
    12001 taaatcaaaa gaacagggca caacttataa aaatcgccta tgtagactct gtaactaaag
    12061 gttacagtaa agcacgtttt gatattgaag ctaaaaaaat tatacagtca tcttcatcag
    12121 aaagctattc attggtatct ataaatattc agaaattcaa acttattaat gattcttttg
    12181 gtagtgaagc aggagataaa acattaaagt acatacatga tgtcattagt aattatctag
    12241 aaccaggaga attgctttgt cgagtttcta atgaccagtt taatgtactt attaaaacta
    12301 ttcctcaaaa agaaattctt aagtacatag aaaatatcac caatgatatt aatagtttca
    12361 ataaaaatga agaatgtaaa tattttatag ctctatctat aggtgtatat aaaattgatg
    12421 acccaaatct acctttagtt tcaattcaag accgtgcaaa tgttgcacga aaaaataata
    12481 aaggaaacat tggaaatcat ccactttata catgtgtttt ttatagtgat ttagagcgtt
    12541 taaaaatgat taaagaaaaa gaaatggaaa atagaatgga ggaagcatta aataataacg
    12601 aatttatagt atatcttcag cctaaaataa gcctcaaaga taatactatt gtaggtgcag
    12661 aagcattagt aagatggcaa gacccaaata aaggtcttat accaccagac gagtttattc
    12721 ctttctttga aaaaaatggt tttattacaa aactcgacca ctatgttttt gaaaaagtat
    12781 gcagtatgat aagaaaatgg atagataatg gagaaactcc aatacctgtt tcagttaatt
    12841 tttcacgtgc taatttaaat aatatagatt ttttagaaaa atacagaatg cttcgtaata
    12901 aatacaacat tcctgctaag ctcttagaaa ttgaattaac tgaaacactt gtatttgaaa
    12961 atctccaaaa attaattaat ataatagacc aaatccatga agaaggtttt caatgttcac
    13021 tagatgactt tggaagtgga tactcttcac tcaatatgtt aaaagaaata aaagttgaca
    13081 cattaaaact tgaccgtgct ttttttagct cacctaatgc agataatatt gctgaaaatc
    13141 atgtaataga atctatagtt aatttggcaa aaaaattaaa tatgaatagt atatcagaag
    13201 gtgttgaaac aatcttgcaa atggattatc ttaaaaaaat taattgtgat atgatacaag
    13261 gatatgtttt ttcaaaacct ctacctcaag aaatatttga gaaaatgact tttggaaaaa
    13321 aactagaaga aaccagtatg gatagttccg tttaaataaa ataaaaaact tgtctctata
    13381 aaataatttt aatcatttta taaagacaag tctttttatt ttccaattta attttatttt
    13441 ctaaatttct tacactcggc ttgttttaaa ttcccttttt aagaataata atacaattat
    13501 catagcaata aaatctgcaa ctggctgtgc caaccaaaca ccatctaaat taaattgaat
    13561 tggaagtaca atgattaatg gtataaataa aataaattgt cttaaaagag ttaaaaacat
    13621 tgaatgttta actttactta tacactgaaa atacacaggt cctacaatag aaatccccat
    13681 tataggcaat gtaattagat ttaggcttat acctcttact gctatatcca tcaaatctga
    13741 atctttatta aatataccta caaatacttc tgggaaaact tgtactacta aaaatccaaa
    13801 tgacaatatg acaactgaca ctatgataga taatattaaa gttttctttg cacgctcata
    13861 ttgttttgca ccataattgt atgatattat agtttgcata ccttgattca atccaaacac
    13921 aggcatcata aacatcaata aaatagatgt aagtgctgtc atagctccaa tagctaaatc
    13981 tccaccataa acctttagag aattatttgt aaacatgtga gttatactta tagctacttc
    14041 catagcaaaa ggtgtcattc ctattgcaaa taccttcttt actatatctt tattcaattt
    14101 taagttacat ttttttagtt ttaaattgga cttccctctt ataaaataat gtattaccca
    14161 aaagaatact actatttgac agagtactgt tgctatagct gctcctttta tacctaaatt
    14221 aaatacaaat ataaatatag ggtccaatat caggtttaat acacatccta caatcattgt
    14281 ccttgctgct aattttggat taccttctgc cctaattgca ttattaagag aaaatgcaac
    14341 taaattaaat atacttccaa ccaatatgac actcatatag tcttttgcat aaaatattga
    14401 atctttactt gcacccaaaa taaaaagtat atcttctaag aaaactaatc ctaatatagt
    14461 tataattaaa gagataatta tagatagtgt caaagcatta cctaatatct tctctgcttc
    14521 ttctctattt ctttccccta actttataga caatcttgtg ctagcaccta ctgataccaa
    14581 cattccaaaa gctattatta gagtaaatac tggcatagtt acaccaagac ctgcaatagc
    14641 tatagagcca ataccttcca tagaaccaat aaaagctcta tctactacat tataaagtga
    14701 agttaccatc attgctatta ttgcaggtat cgaataccta aataataaaa tccatatttt
    14761 ttcatgtctt aatgcttctt gattctccat gttgtcacca tttacaccat gtcataagtt
    14821 atacaaattg gtttatttgt tcttgggtct gttgctattt ctgcatcaat attaaataat
    14881 tcttttagat tatccttcgt cataacatca tatgcatcac cttcacaaac tattttacct
    14941 ttcttaatac ctatcatgtg gtcagcaaat cttgctgcat tattaagttc atgtattacc
    15001 atgacaatag ttcttccttg ttttttattt agttcatcta ataattttag tatctccaat
    15061 tgatgtgcca aatctaaata tgttgtaggc tcatccagta ataatatatc agtttcttgt
    15121 gccaaagcca tagctatcca tgccctttgt ctttgtcctc ctgataaatc ttccatattt
    15181 ctatccttaa attctgcaat tccagtaact tctaaactcc aatttacaat ctctctgtct
    15241 tctttactag aattaccaaa tccactttta tgaggaaatc ttccataaga aattaattct
    15301 gaaacggtaa gaccactagg agcttgaggg ctttgtggaa gtacagccat atcttttgct
    15361 atttctttag acttcttttt gtagatgtcc tctccattta tatatatttt cccactatta
    15421 gggctaataa ttctacctat agttttaagt attgtcgatt ttccacaacc attagcccct
    15481 atgatggtag ttatttttcc ttttggtata tttaaattta aattttttat aatatctgtg
    15541 tttccatatg atacatttag attcttagta tttatacagt tcataacaac accctttcct
    15601 aatcatttac caacattaaa taaataaagt acggcactcc tattatagaa actactatac
    15661 ctactggtat ttctataggt gctagaagat ttcttgatat agtatctgct attaaaagta
    15721 ataatgttcc tatcaatgct gatactggta ttaatctttt atgctttggt cctattattt
    15781 ttctacctat atgtggagct attaaaccta aaaatgaaat actacctgct actgctgtcg
    15841 atacaccaga taaaattact gcatatatta taagtcttct tctttgtttt tctacatcta
    15901 caccaagact tatagatact tcatcaccca aatttaaaac atctatatat ttagatttat
    15961 aaatagttat acccataaaa atcactataa atggaagcac agctattaca tatttccaac
    16021 tggttcccca aatgcttcct gatgtccatg ccataaccct attaaattcc tgtgttgtaa
    16081 atttcaattg gaatattgtc aaaattgaag taaaagctat gtttacacct ataccaataa
    16141 gtagtaatct tgatgaattt ataccacctt tccatgcaag agaatatata agaaatgctc
    16201 caaataaagc tcctataagt gcaacaatag gcattgtaaa tacagtcaag ttactcatac
    16261 cttcatatac attaccattc ataaggtata tataaactac tacaaacaat gcagcacctg
    16321 aatttattcc caaaattcca gaatcagcta aatcattctt tgtaactcct tgaagtatta
    16381 cacctgctgt tgataatgca gaaccaacca aaagtgctat tactattctt ggtaatctta
    16441 atttaaatat tgcaatttca tgattacgct gaccctgccc taaaaaagtc tttataacat
    16501 ccattggttc tatagaaaat gagcccatat ttaaactcaa taaaaaagtg ataaaaataa
    16561 gcaccattaa aattgatata acaagtaatg ttttagaatt tatttttata atatttttca
    16621 tagtacctat ttctccccct ttctaacaag gtatataaat attggcactc ctataagtgc
    16681 tgttatagaa cctactggtg tctcatatgg aggatttatc atcctactta atatatctgt
    16741 atatacaagc aatacagctc ctaatactaa agagctcggt attatatact tataatctga
    16801 acctactatt cctttagcta tttgaggaac tataagacct ataaaaataa tattaccaga
    16861 cactgacaca gatgctccag tcattaatat tacaagtact atacatataa atctaacaaa
    16921 gttagttttt tgtcctaatc ctattgccac ttcttctcca agacttaata tagttattct
    16981 tggagccata ataatagcta aaataaatcc aataccacca gctacaaata gcaattttat
    17041 accttcccac ttcactccta caagccctcc tgatatccag aaacttaatt gttgagatag
    17101 gttaaaatac atagatattg ctgatgccaa agaaattagc aatgtaccaa gagcgactcc
    17161 tgcaagagct aattttacat tattaactct tcctcttgat ttagaactta taaaatatat
    17221 aaataatcca cttatacttg ctccaataaa tgcaaacatc attacactaa aacttccatg
    17281 tataagttga ggaagttttt tttgaagtac cagtgatatc gaaattgcga atgtagctcc
    17341 ttgtgtaatt cctataacag atggttcagc tataggattt ctcgttattc cttgcattat
    17401 tgccccagaa actgctaaaa atcctccaac aagtgcagct gctattgccc ttggaagtct
    17461 aacatccttt actattctca tatttattcc atcttccatt tttatcaaac tatctataac
    17521 agttgataga ttcatatctt ttgccccaat agttatggat aatacaattc caaatacaag
    17581 tacaactata cttactaata tcaacaaata agttatattt tttttactca gcttagtcaa
    17641 ttatcctacc tccttttccc ttttaatcgt cagtataata tcatctatta agtattttaa
    17701 ttttaagatt tattaaaaat aggtgtgaaa aatgaatatc attatctcac acctactaag
    17761 ttaagaatta ctttgttaat tcatttttaa ctgattctaa taataactct tttccaattg
    17821 gattgtatga ttgactgaaa aatggagcag catcaagtat tgttacatta ccatctttta
    17881 ctgctcttat ttgagcccag atagcactat tttctaaatc ttttttatct gcttctgtag
    17941 caattacaac tatatggtct gcatctatat ctgttaaacc ttccatactt accattggta
    18001 gtgttattcc atcctgtttt ggcatattct taggtttagc taatttcata tcatcattga
    18061 ttagagtacc tattccacca tcactaaata ccataaattg acctgaactt gccaacactg
    18121 gtaaataagt tttttctcca ttcttttcta taacttcctt acctaacttt gtagcttttt
    18181 catcatactt ctgtagccaa cttttagctt cttcttcttt gtcaaataac tttgaaacat
    18241 cagttaactt gcttctccag tcatttgcat aatctttcat cataactaca ggagctattt
    18301 cttttaattg gtcatatatt ttttcttgtc tttgactcat tattattaaa tcaggattta
    18361 cttctaatat agcttccata tccatagtat ccatcattga gtgcccaact atctttacac
    18421 ctttaagctc ttctcttatg taacttggta atttatcagt ttcatatgag tctacatttg
    18481 ctgttgcaac aggcttataa cctgctaata atagttcttc actacttcct gatatatcaa
    18541 ctattttttt aggatttgat ggtatcttaa cctctccttt aacagattgt acaactcttg
    18601 tctctgattt atctttatcc tctgtattct tatctgaaca tgctgtaaca agtactaacg
    18661 tagttataat agatataaat attgcaagtg attttatttt tttcatgatt acttctccta
    18721 cttgtttatt atacttacat gatattgatt atcataagca catatttatt atagcatttt
    18781 tctattttta gtcaatatta ttttgtaaaa ggataaaaat tcctaattaa tcttaattat
    18841 atatagctta gataaggttt tcttatatta aacagtattt ttttaaagaa ttggacacaa
    18901 atacaatgac ttttcagtta aacaatgata aaatgcaatt tatagataat gaatttagta
    18961 aggaggattc aaaacatggt acatttagta tattgtgata atgcaggaaa aaaaggagaa
    19021 aaggtcttag ataaaatctt ggctggaaca aaaacaatgg ttattagagg tgctgctggt
    19081 agaaaaatac cacatagtag agtatttgaa gatgagttac tttattttat ggaaaaagga
    19141 actgcaaaaa tatctgcaaa agccactgtt aaaaaggtag aaaactatgt taaattaaca
    19201 gaagatgaaa tttctaaaat tttaaatgaa aatcaaccaa aattaaattt atctgaaaag
    19261 caaaaagttc gttggcataa aaaatgttta tgcttagttg aatttgaaaa tgttgaagaa
    19321 ataacacctc ttgattttga ccatcaagga aatatggatg attggcttat tgttgaaaaa
    19381 attgaagatg tagttgttgg tactagtata ccttacaatt atgagaagtc taaattttaa
    19441 agtatcaata acataatact atattgctaa actctactta aataaaaaaa gagtgcattt
    19501 aataattata aaaattattt ttatacactc ttttttaata ataattttaa attacttact
    19561 ttctaatctt tcatatacat ctataaattc ttctgctaat tgttgaaaat tcatagctgt
    19621 cattaaatgg tcttgtgcat gaataagtat aacacttatt tcagttttat taccattagc
    19681 ttcattttgt atcaattctg tttggaattt atgagcttta tgaattgatt ctttcgactt
    19741 ttcaatacat tctctagctt tatctatatt cccgctcttt gcttctttta ttgcttcaaa
    19801 tgctagccct ttcgcatcac ctgcaaatcc aattattcca aaagatatct ctatcatctt
    19861 ctcatccatt tattcgacct cctaaatatc tctatagctt gatatgacta atccattctt
    19921 ttctaaaaat tctttgactt ttttagatgt aagtatcttg tgctctttcg ttctatcaat
    19981 agcataagat gtagaattta aaatataatc atctaaaaaa gctgggtgag acataatatc
    20041 cactacatca tacttcatta tttcgtctat attttttata aaatattctt cacttacatt
    20101 ttccttataa aaactatcta ttaaaggaac tactttagaa tactctaaat tatattcaaa
    20161 tccacctctt attggaagat tatacttggt tacaatcttc tctattactg gctttaaact
    20221 taataaagta tggatatgat ggtgactatc taaatgagat atatctattc caagcccttt
    20281 taccctatct atttgagcac aaagttctct ataaatctca tcacaatcca ttttttcaat
    20341 aatttcgtct gtaatcctcc taataaaaaa accgtcttta tcaacaatag tttttaagtt
    20401 agataataat ggtctaccac aacttaaagt catatgaact ccacaattta aagttttatt
    20461 ttctttaagt aagcctactc catgttctac cccaggcata tttgccatca tagaagtaga
    20521 cctaactata ccattattat gagctgaaat tattccatag ttcacagctt cacaatatcc
    20581 aaaatcatct gcatttatta taattttagt cataccataa cctactttct atagacaact
    20641 atctattaaa tctaggaaga taatctttat gagcttccaa catttcgtct aatatagttt
    20701 ttgctaagtc atcagatgga atcaatgggt ttatacaaag tgccaaaaga gcagattcat
    20761 aatcgccata tacagctgct ttagctgcca atacttcaaa tgatttgatt tgacttacta
    20821 atccatgcac agaatctggt aaatatccta tacttattgg ttttggacca ttttttgtta
    20881 ttacactact tacttctacc gcttgttcat ctttaaaatc tcttatagaa cccttattta
    20941 atgtattaac tacttggata tcttttttgt cgttgtatat agaacttatt aaattacaag
    21001 cagcatcact atagtaagcc cctcctcttt tttcaagttg aggtggtttt acatctaatt
    21061 tttcatcctt atataattca aataattgtt cttccaattc tttaactact tgacctctag
    21121 cttttccctc tttaaactct ctcaactcat cctcaagcat ttctttagtt ttatagtagt
    21181 atctatgata tggacaaggt attgcaccca aagattttac aaattcagca ttaaaatcaa
    21241 ttgcctttat attttgcata cttatatctg ctttgacaaa tttatcaatt gcttcttttg
    21301 taacatcttc tccatctaaa gatacattta gaccataaac catatgatta agacctgcaa
    21361 aatccatact tattctgtca ctttctacat taaatagttt ggcaacatca ttttttaaat
    21421 gaattggcac gttgcataga cctatatatc ttctaaaatt agtatatttg aatacagcct
    21481 ctgttattat tcctgttgga tttgtaaaat tcaccatcca tgcatttgga cataattctt
    21541 ccacatcttt tattatatca aatataactg gtattgtgcg aagggcttta aataaacctc
    21601 cagcaccatt agtttcctgc cccataactc catgagataa aggaatagtt tcatctttta
    21661 ttctcgcatc taaaagacct actctaagtt gtgttgtcac aaagtctgca tctcttagag
    21721 cttctcttct atctaaagta agatttattt tcatatctac tccagctttt tttaccatcc
    21781 tcttagctag atttccaact atctctaatt tttcttttcc agcttctata tctacaagcc
    21841 ataattcctt tacaggtaac tcttcatatc tatttataaa accctctaca agctcaggag
    21901 tataacttga acctcctcca attgtaacta tttttagtcc ctttttcatc tcattatacc
    21961 atccttacct ataatttaat atactctaca aaaccccata catttattta aaactctata
    22021 catttattta ccgaaataaa actacctagt tgttaatttt caatttatta gcttccattt
    22081 tttcagacgc tatgacaaat ggaatatata ttaatattga taatattaaa ttaaatagtg
    22141 ctaaagctcc accagtccag tcacctgtag ccattgctcc acctaatata ggaggtgtta
    22201 cccaaggtat tttaggtaaa attactggtg ctactaatcc aaaatctatt gctaagtatg
    22261 atacagcact acatattact ggtgcaagta caaatggaat cataaatata ggatttaaaa
    22321 ctattggaag tccaaatatc aatggttctc ctatgttaaa tattgcaggt ggaccaccta
    22381 atactatcat ctctttacgt cttcttccag ctattaataa agctactatt aatcccaata
    22441 ccattccaga accacctaaa tatacaaatg catcaaagaa tgcacctgca agtacatggt
    22501 atccttccgt tgctcctgct tgagccaagg cagcattttc tccacctaat ttcataagta
    22561 tagtttctgt aaaaggaagt gctatatttg caccatgaag tccaacagtc caaagtatat
    22621 gaattataaa tgcaataagc attgtagaac ctagactatc agctacattt gatagtggtg
    22681 ctcctaaata agtatttaaa atatctgtta agaaacttcc attagaaagc atttttataa
    22741 atattcctat tacagtaaat atcattatag ttaacattcc tggtattaac tttgcaaatg
    22801 atcttgaaac tgctggtgga actccatctg gcatttttat agttaatttc tttaccttag
    22861 ataatcttac aaatatctca gttgataata atcctattaa tatagaagag aataaagctg
    22921 ctgtacctaa gtaggtttgt tgtatcattc cccatccttc aactactgta gttcctccct
    22981 caggaactat tttaccaatt tgtggagcca ttataaaaaa gatagataaa gctataagcc
    23041 cagattgtaa cccatcactc tcataagatt ttgctagaaa ataagcaaca cctattacta
    23101 agaatactgc cattgtacct attgctcccc accaaaggtc gcctccaaat gtggtccagt
    23161 tttctccaaa tatacttgcc atcaaatttt tgtatgcttc caatggtaag ttatttatca
    23221 atgttcccaa tgcacctacc atagttatag gtatcattac tataaatgca tctcttactg
    23281 ctactaagtg cctttgagct cctattttag cagctacagg cactatatat ttatccataa
    23341 atgacatgaa tttttccatt ttacatcctc cagtacaact ttattttgaa ctatttaact
    23401 ctacagctct atctaacaca gcttttccat tacatcttcc ataatccatc atatttataa
    23461 cttctactgg aatattatat ggttttgcca tttcagttgc ttttcctaat acatatctaa
    23521 cttgaggccc taaaagtaag acatcacaat tttctatttc attttttaag ttcacttctg
    23581 atatagccca tatttttgct tctataccgt tttcctttgc tgcatcctcc atttttgtaa
    23641 ctaataaact agttgacatt cctgctgaac aagctaacat tatttttatc attttttccc
    23701 ctccaaatta gattcattaa atatttttta agtcataagt tttttaaact atacatttaa
    23761 aatatgtttt tctattaatt ttttcttaat gtatatatct tatttttaaa tgtatataca
    23821 tttaattctt gatattatta tataccaaat gtatatacat ttcaagaaaa ctttttcatt
    23881 ttatttaaat tttattcgac aacagcattc aaagttaatg tatatccata gtgatatgtg
    23941 tgtgagtact caaaaatctc actattttgt aacttacaaa tttggcttat atataaaagt
    24001 ggctctggtt cttctaattt tagattatcc atacactctt cattcgatat tacagcttgt
    24061 acttctctct ctgacatggc tatcttgtat ccacacactt cttcaacaaa attataaaga
    24121 gaattttcac aatgagtctt atttaaattt ggaaataact taactggcat ataagtaatc
    24181 atatatgaaa ctcttacatc attagctaat cttactcttt ttatttccca aacattttct
    24241 tctaaactta tattcaactt acttgataaa ttttcatctg cctgaactat ttcaagagat
    24301 accaatatat tagatacttt atagccttta ctttccatct ctttagtaaa tccacttaca
    24361 gacgatatat tatcatatat attatttgca ataactatac tacctacacc tctttgtcta
    24421 gttatatagc cttctttaac tagattatta agtgcagacc ttacagtcat tctagttaca
    24481 ttaaattctt cactcagctg tttttcagaa gggattaaat ccccttcttt caaactgtct
    24541 gaatttatta actctctaac atgattttct ataactttat aaattggctc tttcatttaa
    24601 ttcactcctt attaaatctt ttatataata agataatttt atatacaaac aaagaaaatg
    24661 tctatacatt taagataatt taaattttat tcagaaatct tgtacaactt tccgagtttt
    24721 aataaagata taaatagact tatcattaat atacctttta atatacttga taaggttata
    24781 ctccaccata caccatttag acccaacatt tctggtctag atataaagta agccaatggt
    24841 actcttgcac cagtcagtat agtaattata atagaaggta tataggttct ccctaatcct
    24901 ttgaaagctc ctgttgttat tatttctaaa cacataaaaa attgagaata gcctaatatt
    24961 ttaagataaa ctgctccttt ttctattgcc tcactttcat ctataaaaat agaaaatata
    25021 ctctctcctg caaacactaa tactaaagta gttattatac caagtacaga agatacagtt
    25081 atagctattt tgcaaccttt atttattcta gaatacttct tggcaccata attttgtcca
    25141 acaaaactgc ctaatgcaac tgatagccct tcagccgtca tccaggaaat tgcttctatt
    25201 tgtgagccta ctttttgtac tgcaacagcc acaggccccc atgatgctac tattactcct
    25261 aatagcattg agaatactgt aaacatacca ctttgtattg ctataggtaa tcccaattta
    25321 tacagaactt tataatattt agtctctatt tctttaaata gtcttaattt aaaatattct
    25381 tctttacttt taactattat atatataaaa catgtagtaa ctactatctg agcaaataca
    25441 gttgcaatag cagctcctgc aacaccaagt tttggagcaa atccccatcc aaaaattaat
    25501 atagggtcaa gtataatatt agttattaat cctactgtat taatacgaaa aggagtttta
    25561 ctatttccaa gaccattgaa aattgcagta aatactggat taataaaata aaataccata
    25621 cctagagcta ctattatcaa atactgtcta gacatggtta taacttctaa gtctcctaat
    25681 ctaaaaaaac ctatcaattg tttattaagt actattaaag aaattgtata tattattgct
    25741 agtataatat ttatttctat tgctgacttc atatatgatt tagtagtgct tatattattt
    25801 gcaccaatac tttgtgctac ttttatctct ccacctactt ttgaaatcat aataaatgcc
    25861 attgcaagcc atggaaaaaa accagctgtt ccaactgcag ctactgcttt acttccggct
    25921 ttaccaaccc atatcatatc tatcatgtta taccccatct gtataaatga agtccccatt
    25981 attggaagtg atagcttaag taatttttct gtaattcttc cttctgttaa attaagtctg
    26041 tctttcattt tccacctctt ttatattaaa tttatttgct tatatagcta gattaatcat
    26101 tttatttgat tgagttcact atccttcaat ttatattatc atatctcagg attgcatctc
    26161 aatcatactt atgtttaaga tacagtattt tacctatcta tgataaaatt aatacttatg
    26221 aatataaatc tagccatttc atcaatacat attctaacct ttaacttaac ttcaattaag
    26281 tatacatatc gtaaccaatt agaatatttt atacggtatc ttccattaat aaacaaccaa
    26341 tatcaatgat ttatatataa ttttatttat acataaaata gtatactata ataataaaaa
    26401 aactatattt gaatgcttta agcatgttta ctaattgtaa tttccacaca aatttgataa
    26461 tatatgaata tattatggtg aaatttatag atatatatta attatgggga gattgtggga
    26521 ggttcttttc acaagacatt taagctatga aaataaataa aaaattcaat agagttgata
    26581 tgcaggtttc aatacttact gcaattattg taataatatc ttcctcatgt gtatattttt
    26641 ttaattatac actaacttat aagagcatga tatactcttt aagtgagagg tcaaaacata
    26701 tctatgaata tgtagaaaga aatattgata aaaatacctt tactgaaatt aattctaaag
    26761 aagaccaatc aaaaaagtct tataaaagtt ctaaaatcct tttagaatct gtaaaaaata
    26821 ctactggagt catgtatcta tatactgcta aaaaaacaaa agatggatca ctagtatatg
    26881 ttgttgatgg tttaaattca tctaggagtg attttagaaa tgctggtgat ttgatagaaa
    26941 aagagatttg gggagacctt aacaaagcat tagataatca tatattgtta cctaaaaaga
    27001 ttaaatcaac atcttgggga tacatattta tatcgtattt tcctattcac aatgatgttg
    27061 gtaaggttgt tggagtaata ggaattgaat ttgaagcaaa acatcaatac aatacattta
    27121 aatttataag tatagttaca ccaataatag cattattaac ttgtctaata tcagcattga
    27181 ttgcagtgat tctatttaaa agaatatcta atcctacata tactgatttt gctaatacag
    27241 attatttaac aggcctaaaa aatagaaatg cttttgaaat agatatgaat aacttaaaca
    27301 attcaaatct aaagaatcta atatctatca tatcaataga tttagacggt ttaaaaaaga
    27361 ttaatgataa atttggccat cagacaggag acttatatat taagcaagca agtaaaatta
    27421 tacaatatgt aataaataaa aaatatatag tttatcgtat tggaggtgat gaatacataa
    27481 ttattctaaa aaatttatct cacaaagaaa taaataatat tataaataat attaatttaa
    27541 aaatagttga agaaaactca actaatgaat taaaattatc aatgtctatt ggatatgcta
    27601 tttttgatga aaaagtagat gtttctttat ttgatacata tcgtcgtgca gattctcaaa
    27661 tgtatgataa caaaaggaaa attaataaac aatatcaata ataaatttaa aaagtctatt
    27721 ctttaaataa taaatcacaa gaatagactt ttttaaattt gtgtttttat aagataaatt
    27781 atttaattaa agtttgttct tttttctaaa cgattcttta taaattcata tattactttc
    27841 actaatatat ttgtgactaa tattaacaca gctagtgatg ctgcctgtgc agtactcccc
    27901 ttatcatcta gttgtacaat tgatattgca gctacttttg aacttgcagt atatataaaa
    27961 actaatgctg atattgtcac catggagttt aggaaaaagt acataaagtt ctctaaaact
    28021 gctggcaaac aaagtggtat agttacgttc ttaagaattg atgcgctgtt tattcccatt
    28081 gatttagcta ccatatccaa ttctttatct actttcttaa tagcagtagt agctgttata
    28141 aatgctactg aaaagaaatg aactatatta catataacca ttataagtaa tgttccatat
    28201 aatccattaa acaaattctt aatgtatatt gtatccataa aattaaactc taatttatta
    28261 aaaaataata cataagctat acctaaaact aatcctggca atgccatagg caatgtactt
    28321 aaaaaatacc caaatgattt taaatatgga gctttttctg ttttttcatt caaatatgca
    28381 aataagaaaa caaagctcgt ccctagaata gctgtcaata gtgacatttt tattgaattt
    28441 ataaatggtt taatcccatc aatactgttt ttcatagaaa aatgttccag tgtcagtctt
    28501 aaatcatatg gccaaactga tattaatgaa gataagatta cagttaacat tacagctatt
    28561 ataccaaatg caataattcc tacaacccct cctaaaatac tatctcttaa cttattagtt
    28621 ttaaccttat atggaattga ctttgaagtt atatatgaat tttgattttt ttcaattttt
    28681 ttagttacta taaacgatat tattgcaggt attaaaagta atattgatac agttgctccc
    28741 attggcatat tctgttgccc tataatttgc ttatatacat ctattgataa tacattaaac
    28801 tctcctccta tcacctttgg agctccaaaa tctgtaaaac ataatgtaaa cgatacgaat
    28861 gcagaactta ttaaagagta ttttatactt ggtattgtta tgcttataaa ttctcttatc
    28921 ttgcttatcc ccatagtatt actagcttca tatagtctat aatcggttga acttagagat
    28981 atataaagta tttgaaatga ttgaggaaat acatatataa tttgtgccaa tattattccc
    29041 actgctccat atattggaac atgcactcca agtaaattag ttaccaaacc ttgatttcca
    29101 aataaatata ctagacctat tgcatgcatc attgttggag caaacatagg aaacatagct
    29161 aatgatttaa aaaaaccttt aaatttaatc tttgttctag ttaatgcata tgcatatact
    29221 aatgcaagtg ttagtgatat tacagttgat ataactgata caaatactgt attttttaga
    29281 gaatttaata gagattcaga agccaagtat tcttgaaagt tttgtaatcc tacaaagact
    29341 ccatctttat ctaaaaaagc ctgtacaaat agatatatta taggtgctaa taaaaatatt
    29401 actaaagaca taactaatac tgtaagcata cattttttta tatctaaatt catttttgat
    29461 ttaagactta tacttttaga acttatagca ttattattca tacacttatg ctcctttaac
    29521 ttttggatac tttaaatagt atttttcgtc tatacttatt tttacaaggt caccttctac
    29581 taaatttaaa ttaacccact cttttataga agcatcactt attatgaaat tttcatttaa
    29641 tttatctttt atttcaactc tcaataaatt ccctctaaac tctatatttg atattattcc
    29701 ttggtagttt tcttttgtcg atttctctat ttgaacatat tctggtctta cagttaatat
    29761 agaatctcca ttatcaaaac agtttgtatc tcctataaat tgagctgtaa ataggttttg
    29821 aggattttgg tatatttctt ctggagtccc tatttgcatt atatctcctc catttagaat
    29881 agctatttta tctgccatag ttatagcttc ttcttggtca tgtgttacca ttattgtcgt
    29941 tatattaagt tctttttgta atcttttaat gtccattcta agcttgtgtc taacctttgc
    30001 atctaatgcg gacattggtt catctaaaag taaaaacttt ggttcaagag ctaatgctct
    30061 tgctattgca atcctctgtt gttgcccacc tgataatgct ttaggatatt tatgagcttc
    30121 attagtcaat ccaactgttt caagtacttc cttaacttta ttatctattt ttccttttga
    30181 aacttttctc tcttttagtg gaaaagctat attgttataa gcagtcatat tcggaaataa
    30241 tgcatatgat tgaaatacta taccaaaacc tctctttgat ggctctaaat ttgtaatatc
    30301 tttatcctgg agaattatag ttccgctatt tacatcttca agacctgcta ttattctaag
    30361 taatgtagtt ttaccacatc cactaggtcc gagtaaacat agaaattctc cttcttcaat
    30421 atccaaacta atattattta aaaccctttt ttggtcataa cttttaaaca cattatttat
    30481 ttttaagtaa ctcatatctt atctctcctt atatagttat ttttttattt taaattttat
    30541 atcctacttt ttcttctaaa attctttcat ctaaattatt aatttaaact ttacttactt
    30601 tcagattttg aaccgtattt tgattcccaa gttttaagta ctgtacttct gtttttggca
    30661 gcagagttta aatcaacttc ttttataagc tgttcaaatg gttgtttaga ataacctttt
    30721 ggaactggat tatctgtctt tatggaagta actgctactt catgtgcata tctatccata
    30781 gctgaatcac ttattgccca atctaaaaat agctttgctt ctttttttat attatctttt
    30841 ttaactaagg catttgcttc taagtcccag cctgaacctt cctttggaaa tacaacctct
    30901 ataggttctc cactagataa ttgttgtgta gctctatatc caagtgtaat acctattgga
    30961 tactctccag agccagctaa cttcgcaggt tttgaacccg agtgtgtata tgtggctata
    31021 tttttatgta atttatccat gtaattgtaa gcatcttttt cacccatcat ttgaataagc
    31081 cctgatactg taagataccc cgttccagat gatgctggat ttggcattgt tataagtcct
    31141 ttatattctg gttttattaa atcttcataa ctttttggaa tatctatacc tagtttctta
    31201 agttccttat aattaactac tattgctgtc tctgggacac tcattcctac ccaagataca
    31261 tcatcttcag tatctttaaa ttttttatca actttatcat aatttttagg cttatatgct
    31321 tcaagtgcac catctttttt tagctctatc atgttcattg ctgataatcc ccatataata
    31381 tctgcttgtg gattatcttt ttctgctaat agttttgaag ctattacacc atgagaatcc
    31441 ataactatct ctacatctat attgggatac tcttctctaa aaccttccat ataataagct
    31501 atctgctctt cctctagtgc tgcatatatt gtaagtttat tatttccttt tccttgagta
    31561 gtttgtggtg ttgttgatat gcaacttgtc ataaatgtaa tcactacaat gcacagacaa
    31621 gtaaatataa attctttcat atcttccccc tttgtgtttt ttcctttttt tatttgtttt
    31681 ttattgtttt tgcttatgtt tatataatac acctaatttg ttaatataaa gttaagtgta
    31741 aattaacagt tcgttaattt ttagaaattt ttgttaaata tattattttt agttaaatac
    31801 agtctgtttt taaacaatat attaattttt atagtatcta cattttaatt taaacataaa
    31861 aaataagtct aacctctata aagtagaatg ttagacttat ttttataagt atttaattat
    31921 acaaccaaat catttttttt cttttcttca agtgcttttt taagcaccat atccttaacc
    31981 ttcataagtc tagtagtact tgctctctca ttttcttcaa gcttcatagc tatatactta
    32041 atagtctcaa tgtaatttgg tatcattaca ttttcaagag cattaacacg tctacgtgtc
    32101 ttttctatct cttgtgcaag aagctgtgca cttttttcac tttctgcaag tcttaaaagt
    32161 ggttgcatag catctgagaa agcttccata gcagaatcta attcacctga tgtaaaagca
    32221 agaccataag gataaatatc actttgattg ttttcagttt taaagtcaaa cacaggaaca
    32281 tcaacactca ttatatttct cgtagaaaca tctactgaaa ctgattgctt tggcatcata
    32341 agtgctgaac ccagatactc ctgactcata actgctcttg cgattataaa gtttttataa
    32401 gcagtatcaa gagcattttc tgcttcttca cgcaatcgct tattttctct tacaatctca
    32461 agaaattgtt tcataagttc atcaagttta tctttaagaa gcttatgacc tcttgtagca
    32521 gttttaagga gtttcttaag tctagtcatt tccatacgtg ttggatttat atttaatcta
    32581 gccacagata tcactcctta ggcatatatt tttcaaaata ttcatcacgg atacgtttaa
    32641 gttcactttt tggtaacata ctaagaagct tccatccgat ttctaatgtc tgctcaatag
    32701 ttctatcagt tctaaatcct tgtgaaacat attctttctc aaattcatct gcaaacttag
    32761 catacattaa atcaatctca gaaagtgcag attcaccaag tatagccata agttcttttg
    32821 catccttacc tcttgaataa gctgcaaaaa gctggttcat tgtatctgca tggtcttcac
    32881 gagtcttacc tttaccaata cctttatctt ttagacgaga aagtgatggt aaaacatcta
    32941 taggaggctg tatatctttt ttataaagtt cacggctaag tataatttgc ccttctgtaa
    33001 tgtatcctgt aaggtcagga attggatgtg tcttatcatc ctcaggcatt gtaagtattg
    33061 gaatcatagt tatagaccct tcatgccctt tcatttttcc tgctctttca tacatagtag
    33121 caaggtcagt ataaagatat cctggatatc cacgacgtcc tggaacttct tttttagctg
    33181 ctgaaacttc acgcaacgcc tctgcatagt ttgtaatatc cgttattata acaagtacat
    33241 gcatatcttg ctcgaaagca aggtattctg ctgctgtaag tgccattcta ggtgtagata
    33301 cacgctctac agctgggtca tttgcaaggt ttacaaacac tacagaacgg tcgattgcac
    33361 cagttgcttt aaaatcgctt ataaaaaatt cagcatcttc aaaagttata ccaactgcag
    33421 caaatacaac ggcgaactta gaatcagttc cacgtacttt tgcctgtctt gcaatctgaa
    33481 ctgcaagatt ggcatgtggc aaaccagacc ctgagaatat aggaagtttt tgccctctaa
    33541 ccaatgtatt aagtccatct atagcagaga ctccagtttg tataaactcg gccggataat
    33601 cacgtgctgc tggatttatt ggagctccat ttatatcaag tcttttatct ggtataattt
    33661 ctggacctcc atcaataggt ctacccattc cactaaaaac acgacctaat atatctggtg
    33721 aaactccaaa gtcaagactc tttcctaaaa aacgaacttt actttcagca aggtttatac
    33781 ctgttgagct ttcgaaaagc tgaacaagtg cagtatctcc atttacctct agaactttgc
    33841 aacggcgaat ttctccattt gaaagttcaa tttctccaag ttcatcaaat tttacacctt
    33901 ctacgccgtt tattaacata agaggaccgg ctacctcttg tattgactta tattccttta
    33961 tcatagttct tcagcccccc tctttttaat aaggtctgca agctcgttat ctatttcatc
    34021 ttcaattgaa tcgtaagctg catctatatc gccttcttct acatacttag cacgaccaat
    34081 cttttcaaga actgaaagct tgataatatc gtttatagta acattttgct tgatagcatc
    34141 ttgtgctgac ttatagaatt ttaatataag tcccatcatt ctatattgtt tatgaagtga
    34201 tgtataagta tcaacttcat ggaaagagtt ttgatgaagg aagtcttctc ttatagaacg
    34261 agtaacctct agtatcaatc tatctccatc tgaaagtgaa tcaaccccaa ccaattgaac
    34321 tatttcttga agttcagatt caacttgtaa aagtttcata gcttgagctc tttgagctga
    34381 gaaattatca tttacatttt tatctgccca tacatcaact ttttcctgat agagtgaata
    34441 actagtcaac cagttaattg ctgggaaatg acgtttatat gctaaagatg catcaagacc
    34501 ccagaatact tttactatac gaagagttgc ttggctgaca ggttctgatg tatcaccacc
    34561 aggaggagaa actgcaccaa ttgctgtaag tgttccttct ctttcatcca tacctagaca
    34621 atttaccttt cctgctctct catagaattg agcaagacgt gaacctaagt atgcaggata
    34681 accttcttca ccaggcatct cctctaaacg accactcatc tctctaagag cctcagccca
    34741 tcttgatgta gagtctgcca taagtgcaac actatatccc atatctctaa aatattcagc
    34801 tattgtaata ccagtgtata tagaagcttc acgtgcagca accggcatat ctgatgtatt
    34861 tgctataagc acagttcttt gcataagtga ctcgcctgtt cttgggtctt ttaattcagg
    34921 aaattcaaga agaacatctg tcatttcatt tccacgctcg ccacagccta tatatacaac
    34981 tatatctgca tctgcccatt tagcaagctg atgctgtgta acagtttttc cacttccaaa
    35041 aggtcccggt atagctgcta ccccaccttt tgtgattggg aaaaatgtat ctataactct
    35101 ttgacctgta acaagaagtg aatctggtgt tttcttttct ctatatggtc tttcttttct
    35161 aactggccat ttttgcatca tagttacagg tatatcatta cctttttcat ctgtaataac
    35221 acaaactgta tcttcaacag taaattcacc agaatatatt tcttttatag tacccttaac
    35281 tccataagga accataattt tacattctac tactgctgtt tcttgaacgc caccaattat
    35341 atctcctgct acaaccttat caccaacaga ttttttaggt ttaaattccc acttagttgt
    35401 tctatcaagt gaagatactt caacaccttt agttatatta gtacctgaaa tatcatacat
    35461 tttttcaagt ggacgttgaa taccatcata aatagttgaa ataagacctg gtccaagttc
    35521 aacactcata ggcattccaa gagaaactac ttcttcccct ggtcctagac cggaagtttc
    35581 ctcataaacc tgtatagaag ctttatctcc atgcatttct ataatctcgc ctataagatg
    35641 tttatctgaa actcttacga cgtcaaacat attcgcatct ctcatgccct ccgctacaac
    35701 aagcggtcct gacaccttta caattgtgcc tgttttcata ttctctcacc tgcaatcata
    35761 tactttatca aaatttaaat attccctcct atattttatt agcacatata tttagcacca
    35821 ataaaatttg agattgtctt ttattcaact ttcatctatt ctttaaataa tatatctgca
    35881 cctatagcac gtttactaga ttctcttact tcattcatac ctaatcccat acttcctcca
    35941 atacctggaa taactataat ggcaggaagt tggtaatctt tgtattttgc tattgattct
    36001 tttgcaagca atgatgcctg ttctgtaata taaattatcg catattcatc ttcaaccaac
    36061 ttatgtatac tttttttaat ttcgtcacta tcataggctg ggaagatatc tattccaaga
    36121 gctaaaaatc ccatgatgct atctttatct ccaactactc ctaccttata cataagcatc
    36181 ccttagcctt tcttttatag tatctgcgtc tatattatta agcttactag tcaaaataat
    36241 tctaattgtt tttatctctg attctttagc atataaatat gcaattagtg gttctaatgt
    36301 aagaggctta aattttgctc ctctaacata ttccataaga tagtcatcac aaagtttttc
    36361 aaagacagta aaaccactat ccatgccatt tttacagaca tcagagtaag aagtagattt
    36421 aaaacatgat gctggagtat ctgaggaaaa agcttctaag aatttttcct tatcaagtga
    36481 gccacctgaa acaaacacat tcataaacat atcaaaatct ttcttcatat ttttgactct
    36541 tataaaactc tttagatttg ttaaatcaca aactttcata acataatcta ttacaaattt
    36601 gttttggctt tctttagcag tttctttcat actggtaaag gttgctttat ctagaacaat
    36661 atcaaccatc tggccatttt gtgtcttatt ataaacatca aatgcctcta atatagctga
    36721 tgacattatc ttaggtaaat cactaaaact acgatttgct aaagattctc taagcttaat
    36781 aagaggaatt gtacctccat caattaaata tttctcacca tctacaccag aaatttcttc
    36841 tttaatcagc acttttaaat tgtgatagtc atttttataa agaaatacat caacaaattt
    36901 ttcttcagga gctagacttt taagaatttc atatgtcttt gataattctt gagtcaaaat
    36961 attctcaaaa ttatgtatat tattatttga tgtatcagca taacctgcct cagctataat
    37021 tcttaaagag tcttcagggc ttttggcttc tgccatttga ataaacattt gtctgttaag
    37081 aagctgtttt tcaagcactc ttattcgtgc aacggcatag ggataagttt tttcttctgc
    37141 cattttttaa cccctcctta attaaacaaa atttctgctg caatttgttc aatatattca
    37201 tgctctactg caattatggc ttcaaatgaa taattgtact caatgtcacc ttttttaact
    37261 atgaaacctc cagtaatatc tctagtttca tcagacactt taatgccttt ttttgatagc
    37321 acatctttta atgttttttt atctttttta gataatacta tctctgaatc atctgtacaa
    37381 tttgattttt caatcatgtt tagtataatc tcttcatatt cattatcttt aagatttaaa
    37441 agtttgtttt tagcttctaa aattacctct tctaaaatct cttgtttttt tgataatatt
    37501 tgctttttag cctccatata agctcctgat atttccttag aagccgcttt ttctgcttct
    37561 gcctctgcaa gctttgtata ggaagccatt tccttttcag ctttttcatt agctgaattt
    37621 acttttaaat ctgcttcact tttagcttta tccaatattt cttgtgcttc ttgtttggca
    37681 tcagctataa ttctgtcaat cattttttgt tcattaccca taggagtgct tcctcctttt
    37741 catttttttt acttatgaat tataatttat aattaaccaa ttgcaattcc attgtaagct
    37801 aagaaagaaa caagtagtcc taaaagagca tatgtttcaa ctattgctgc aaatgttata
    37861 gctttaccaa gttcttcagg tctttttgca actattccaa ctcctgctgc tgctgcttta
    37921 ccttgtgcta tacctgatac taaaccaact aaaccaattg gaagtcctgc cataagaagt
    37981 tgtaaacctt gagctgatgt tggtattgca tctccaccaa taattcctgc ttttgataaa
    38041 ataaggaaag ctatgataaa tccatacaga ccttgtgtac caggaagaag ttgtaaaact
    38101 agaagttgac cgaatttact agggtcttct gttacaacac ctgctgcggc ttgacctgct
    38161 attgaagtac ctttagcaga acccatacct gcaaataatg ctgctatagc agctcctgat
    38221 attgccaaaa attgaccatt tcctaacgcg tttaaaaatt ccatatttac atctcctttt
    38281 tattaatgct tgtatatttt gttttatact taaacggaac aaaaggaact cctccgcctt
    38341 cataaaactt attgaaaaat tctacatact gtaacctact tgtatgaaca tatgcaccta
    38401 gtgcatttat aagaaggttt attgtgtgtc ctacaacacc aattacaact gcaagtatcg
    38461 gaccagctat agctcctaaa aggttgataa cttgtgctat aactcctgtt gttaaacaaa
    38521 gcgccataat tcttgtataa gataatatat cagcaaaata acttgttata ccatagagac
    38581 ttgaaaatcc tttaaaaagc ttaacaggaa tacctttaaa gcttcttcct tgagtaagta
    38641 caagacctat agcacctata attgctaaat attttcctac ttctgaaaat acacctatat
    38701 cccctgcaac aaaaggtatt ataagtagac atacacctgt tatacacaaa taccaaaaac
    38761 ctatgtcaaa gattgcatct attacctttc cacttcttat taatgtatat gcttttattc
    38821 ccaaacctac atatatatgg ataataccga acagtaacga cattcccata agtgtcataa
    38881 catcctctaa aggattaatt aatgctttta taggaattaa atcccccaaa atacccccga
    38941 atataagacc ccatacagta gttgaaactc cacagatacc aataagttta attaaatttc
    39001 cttcaccttt ttgtatcttt aatttatata caacaaatcc acagactact gcaattatta
    39061 acccatatgc ggcgtcactt agcatcattc caaagaatac aacaaagaag aaagttaaaa
    39121 ttgtatttgg gtcaatatct tttgtacttg gatagctgta catgttagtt acactttcaa
    39181 aaggtgttac taatttattg ttttcaagca gaattgggaa gccttcttct ttacttcctt
    39241 cttcagtctg aacacaacaa ttaaactttt ctgtaatttc tttaattaat tcatctgccc
    39301 ttttgcttgg taaccaacca ttaagacaga atgttgtttt tgttttgaca agtctctcta
    39361 taatcttttt ttggtctctt tctatagtgt aaaaatcata taggttttca agcatgtgta
    39421 tactatctgc atgtctttta atttcctctg ttttgtcctt agaaacgttt tcctgtctaa
    39481 ttatcatttc attgtatttc ttgatagctt gtaacggtgt accttcttca tttggtaatg
    39541 aaaccactga aaaattaaat tcttttaaaa cttctaaaac atcatcaaaa gtttctttta
    39601 aagtaattaa ataaacatag ttcattaact tgtcagaatt gacaatccca ataacacttt
    39661 ctggtatttc atcttctaat tttactctta aagcatcaat gtttgcgctt atgggcacag
    39721 ttccaagtat agtttttgta tacttagttt ccatattttc aagaggaata tcaaagttca
    39781 cccatggttc taacatactt ttattgttgt taagtaagtt tatgttagac cttattgtac
    39841 ctatctcctt gtgcaaattg tttaactcca gagcttcagc ataaacttgc ccttcttttt
    39901 cactatcaat tttgttaaac tctctttttg gtgcaagtag ggattttttt tgtttcacta
    39961 gtgaagaaac aatattaatt gcttggttta catgagacat tttctgctct agcattataa
    40021 cttcagattc ttcactatct tttacaagat aattttttaa ttcctcctcc tcagtaagaa
    40081 aggcactatc atcaatctga acaaacccac ggttcataag tagctttaaa atctggcttt
    40141 tttgagcttc taagccaaca atagatattt tgttcatatt aactattgcc atattatctc
    40201 actacctttc caataaccgc ttcaatagcc ttactttttc tttcttcagc agttttttca
    40261 attttcagac attcactttt agcattagct aagattttat caacctctgc tctactgtcg
    40321 gtgactgcct tctcaataag agtttcttcc ataagctttg cttgtttttt tacatcagca
    40381 aatatgcttt cacatttgtt tacagcatca gctctgatag actctgcatc agtaacagct
    40441 tttactttga tactgtctgc tgtctgctct gcctcaacaa tgctttttat tatttcaaaa
    40501 gccatccaaa tcacctctct taccttttta ctatgtatat aaccaattag atttttttat
    40561 tctaattaga ttactacctc attgttaaag tataaacagc atttttatat aataattaat
    40621 atttttcaac gtttatttct taataaaaca tattttagaa tagtacttgc ctagatttac
    40681 atcctttttt gacttacttt tagactatga catgagtatg gtttttaaat accattattt
    40741 tcaataatta taatttaaag cgatatagtt tgtcaatagc gttattttgt tttaaaatta
    40801 acgttatgtc gacattttaa atttttattt tatttttttt ataattttaa tttaatttat
    40861 tacatataaa agttttttat attctttttc aacctctaac cattgaagtt tattgttttt
    40921 tttgaatttt tagaaatatc tcgcactatt ttatctaact tttataaatc atcaagctat
    40981 atcgattttt gtcaccttat ttttaatcta atttttttat atctagttag aaataaatca
    41041 atatttttaa agattgataa aacttacacc tagttctaat agttttattt taaggcaatg
    41101 tattaatctt aagtagataa tatcattagt tactctatat attcgacaac attataaaaa
    41161 aatcctcgta ccaatcactc caatcttcaa taggatatgc tgctcctcca tttacacttt
    41221 tcagtagatt ggatgtatat tcttctccca tagaaaaaag ttcccctact gtcatatcat
    41281 aacctctaga tgtaacaacc ttacaaaatg aatccataac atcacgttgt tctcttgttt
    41341 ttataagttc ttttctaaat tcatcatcat tcttagcttc ttctaaaatt ttagttaatt
    41401 tttcattcat tatgtatttc tccttttaag tagtctttct gtaaacttca ataagcttta
    41461 tataaaaagg aagccatcct aaataaacta agatgacttc gcttaactgt tagcaacaca
    41521 tttactagta taaaataatt ggtgtacctg gttctatatt ttcatatatt ttctttgcct
    41581 tagaaaaagg tgtatttaca catccatgtg aacctgaaga cttatatata cttccaccaa
    41641 aagaatttct ccaagtagca tcatgaatgc ctatgttccc attaaacgga agccaataac
    41701 ttacaggtga actgtaacct tgaccagtta atgaaacatt tctagcctta taattaagag
    41761 gatatatacc tgatggtgta gcaaatcctc tagctaaatc acctgttaca acgtctgttg
    41821 atacaagcat ttctccattt ttaaagaacc acatatgttg gccagaaaga ctaatctcta
    41881 catatgtatt tccaatatca tctttattac gagatacagc tttttgtatg taaataggtt
    41941 ctcttgtaac atttttacca gcttttacaa catcaactaa tgcttcagct tctttctttt
    42001 tatctatcaa ccaaccataa attccacctg aaattgtaat atctttacct gtggcagttt
    42061 taaatggtct tgagtcacca aaagtactgt attttttact taaacctctt gtatattcta
    42121 ccatagcatc attatcaaaa gttatgtcat aattcttttc tgagtctact tttaaccatt
    42181 tattaatttc agaaccatct aaagtttcct tacggtcact aaaatcatat gtaacgacta
    42241 caccaacata cttatctaat gtcttaatag cttgtttcaa cttttctgaa tcctttgtgt
    42301 tgtttggtgc ttcataaact ttactttcat ccagagaaat agaagtattc ccagaattaa
    42361 ttgagttttg tactaactta taaaaatcat cttttttcac tttgtttcca agtacctctg
    42421 gtactataac ataagcgttt ttagtgctat catacttagg ataagcactc ttaggttcta
    42481 caacattttt actatcaaaa caagataaag cattaaacct tttttctagt aaatctttat
    42541 tatatgaaag agttatcata ttattatact ctttagactt aaatattcca ccaatccatc
    42601 caatagcgct ctgactatct tttatctttt gaactttatc atctggtgta aattttaaat
    42661 caatatcttt tgcttttatt tgttctttct tattgtttct ttcctctaag tttaaagtat
    42721 aggaattagt actagatgtt aacaaatcat ttacttgctc aactgttttt cctgaagcct
    42781 ttacgccatt aatagtcgtt ccaaagtaaa aatgatttct aaaaaagatt gccattccta
    42841 tataagctac aaacaaaaca cctactacta tagctatctt aaattttaga tttttttgtc
    42901 cactttcttg ttttacctct tttccatcaa tcataattaa atccttcctt acattgtaat
    42961 tacctatgta ccttattggt ttttaaacaa aaccatgtat gccttatttt atatacccaa
    43021 attactgtat cattatttat atttttaaat atatagttat ttctataaaa ccattttatc
    43081 ttttagataa aatgattatt ataaatcccc cctttcaaaa tcccttaaac acaatctctg
    43141 ctatattaat atattaccat acttttaata atcgtgggac tatctatcta tttttgtaac
    43201 actaaactac tattttaaaa aagaaactaa ttttgtgtta gtttctttat cttaacttat
    43261 ttttatattg aattttagct taacataacg ttgttatata gattctattt aattatttta
    43321 gtttctatat cttttccatc tgcatattct ttaagtaatt ccttagcata tccaaaatat
    43381 tcttcaatat cagattcttg actaaaagaa aatatacaaa ttaatatttc atttgcatct
    43441 tctgttatca ttgctctttc agaatcacta gttggactcc caaatttacc cactgaatca
    43501 gataatatag gaagattttc tatatttatt aattctttcc caataccctt atactgttct
    43561 ccttcatctc ctacagtaaa acatactggt gaatgtaatt tgcttacatt atatgtacct
    43621 actggatata atgattttat tgatattaaa ttgtttattt ctactatatt atttattgta
    43681 taaagaccct tcccttgaat aattcttcta accaatgctt ctgaagatgt tctatatcta
    43741 cttggatttt ttccaagctc tttataagca tttcttgcat ctttaatttt atctagtttc
    43801 gtaatgtctt caacttttag actttgattt atagtatcac aaaactcatt tatttcatta
    43861 attaattttt ctgagctttt atttactgct acttttgctt gaatgcaacc cagcttagca
    43921 ttaggccatc tttccttaag tttgtcatca atacttatat ctatcatact taaacaactc
    43981 ctatacacta aatttttatt aaagttttta tcatctatac attatagtac tattcttttg
    44041 ttaataacat gtttattagc aaaataaatt tatttttaat ataattttta taaaatacaa
    44101 ttattttttg ctttatatta gacaatacat tgataaaata aaaaaacact aattatttag
    44161 caatgattta ttaaaatcaa gcattcataa tagtgcttta tataaaaatc aaattttgtt
    44221 ttaaagtaaa tctaaaaaat tcttagctgg cattggtcgt gcaaaataaa atccttgaac
    44281 taaatcacat ccaaattgtt tcattaactc agcttgttct ttactctcaa caccttctat
    44341 aattacaact aaatctagtg attttgctag agacactatt gattttgcta tattgcgact
    44401 tctctcatca tgttcaatgc caccaagaaa agacttatct agcttcaata tatcaattgg
    44461 catatctctt aaacaactaa tagaagaata tcctgtacca aagtcatcca tagaaataga
    44521 aaatccatac gtcctcaact catttaaaac attctttaaa atatttaact cattaatagc
    44581 cacagtttct gttatctcta tttcaatgaa atctgaactt aaattataca tttttattgt
    44641 tttattgata aattttacaa tatctggttg atacaactcg accctagata aatttactga
    44701 aactggaact atctttttac cttgcttttt ccattcatat aaatcattgc aaaccctttc
    44761 aaatacaaat ctcccaattt ttaaaatcaa cttacttttc tctgctattg gaataaatac
    44821 tgcgggactt ataaaaccat gttctggatg attccatctt attagtgctt cactccctat
    44881 catttctcca gttacaagtg aaaacttagg ttgataatac acttcaaatt gattttttac
    44941 caatgctatt tttatatcat ctaaaatcat tgattcctca ctaagcttat tcctaacatc
    45001 ttcattataa atagcataat ttatattttt acctttagca acagacctag ccatatttgc
    45061 tttatttaca gccttttgaa tatctacttc accttcttca acaaaataaa tacccacaca
    45121 cagagaaatt tcaaagacta tactcaaatt actttcaata ttacttcgta tattatcaag
    45181 ttttcttata attttatttc ttccattttt ataatcacag aaaattgcaa aataatcact
    45241 tgaaagtcgt gcatatacct caccttctgt taattcttct tttaaagctt ttgctattgt
    45301 aattaataaa ttgtcaccaa attcatatcc aaaagtatca ttaatcatct taaagttatt
    45361 aatatcaaaa tatactataa tataatttaa agatggttta tctaaaagat acttactaca
    45421 ttctctataa aatttatttt gatttcctat atatgtaact ttatcttcat aagccaaact
    45481 taatatttct ttttcattag atttttttat ataaattata taaaaaataa ttgcaataaa
    45541 tattaataca atagataaca ctgcatatac agctctgttg attatttgtt ttgaattact
    45601 aaatacaaca gtttctggaa ttgaaaaaat taaattccag tcttttatat aattattttc
    45661 actattccca ttatccatag tggcatatcc taaatatctt ctttcttcca acatatttaa
    45721 tacagttgca cctgttttat tttcttgcaa ttcttttttt aatctattta cttcttgaat
    45781 atcattatcc tgttttaaca atttataaat attttttgat aggctatccc cttgtggatg
    45841 aaggataatt gtaccattat tttttacaac ataagatata ctattatttt cataaaaaga
    45901 tatattaata agtttcatca gagtattgat attgtaagta caatataaaa ctccaactac
    45961 tttatcatta tttaataatg gtactgaaat agcaatcttc tttatagact tatttacttc
    46021 atcaacatat ggacttgaaa catacttctt acctttaatt gagtttttga aaaattctct
    46081 atctttcact gaaaacttat cacctttggt attttcagca ttaccattta tatcaactat
    46141 tgccatagtg taaaattttt gattttccat ttcatctaaa agatttttca ttttttcctc
    46201 attaggtata tctggatttg aagcataata gttagaaaaa atatttaaaa catctaaatc
    46261 tttttcaatt tgtctttgtg ctagttgaga gccttgtact gcaacttctt ttatatgtat
    46321 ttttgcctct gacataagaa gatgttttgt atcttctata tagatatacc ccattgtcaa
    46381 aaataatgat gttaaaaaaa gaattgtaat taatgtaaaa cttttatttc tccctgtcat
    46441 tcgactcctc ccctaattag tcaatacttc atatagataa tatatccttt ttataccaat
    46501 tcatcattta tattgtatat aactattctt aaatgttaca cataaaatca acataataac
    46561 cataaaaaca ttcttttatt attataataa cactatttaa ttgaatttta aattaaagaa
    46621 taaaaactat ccattttctc aaacaaaaaa actcttatac aataaaactt tgttttatgt
    46681 ataagagttt ttattttatc tattttatag caaacttatc ttccataata tgaatttaaa
    46741 tacatatctt ttatttcatt cataagtgga tatcttggat ttgctcctgt acattggtca
    46801 tcaaatgctt gaattaccat ttcatctatt gaatccataa acttagattc ttcaacgcca
    46861 aactctttta tagatttagg aataccaact ttaagtttta aatcttcaat agctaccaat
    46921 aaactttgga atttctcttc atcagaattt ccttttaatc ctaaaaatga agctatttca
    46981 gcatatctct ttaatgcttc tggatattta tattgagaga atgctcccat tttctttggt
    47041 gcatctgaag aattaaattt cataacttct gttatcaaaa gagcatttgc aacaccatgt
    47101 ggtacatggt ggaaagcacc taatttatga gccatagagt gacaaacacc caaaaatgca
    47161 tttgcaaaag ccatacctgc cattgtagaa gcatttgcca ttttttctct agcttctggg
    47221 tcttttgcac cattatcata tgcacgtgga agatattcaa atatactctt aatagcttgt
    47281 aaagctaatc catttgtata atcggttgca agcatagata catatgcttc taatgcatga
    47341 gttaaagcat ctataccaga agcagatgtt aagctttttg gcatattcat catcatatca
    47401 gaatcaacta tagccatatt tggcatcagt tcataatctg ctaatggata ttttactcct
    47461 gtatcttggt cagttataac agcaaatggt gttacttctg aaccagtacc tgctgatgtt
    47521 ggtatagcta caaaattagc tttttcaccc attttaggga atgtatatac cctttttctt
    47581 atatccataa atctcatagc taggtcttgg aaatctacat ctggatgctc atacaatgtc
    47641 cacattatct ttcctgcatc cattgcactt cctccaccta tagatattat tacatctgga
    47701 ttaaattctc tcatagcttt agcgcctatc ttagcacatt ccaaagttgg gtctggttct
    47761 acttcaaaga atgtagtgta cttaatattc atttcatcta ataaatcagt aactgcttta
    47821 gtatatccat tattgtaaag gaatgtatct gttacaataa aagctctctt cttattcatt
    47881 acatctttaa gttcttttaa tgctactcct aaacaacctt ttttgaaata aactttttct
    47941 ggtgctctaa accaaagcat attttctctc ctctcagcta ctgttttaat attaagtaaa
    48001 tgcttaactc ctacattttc agatactgaa tttccacccc aagacccaca tcctaaagta
    48061 agagaaggag ctagtttaaa attatataag tcccctatac caccttgaga agaaggagta
    48121 ttaattaaaa ttctacaagt tttcatggct gaagtaaact tatcaagttt ttctctttga
    48181 tttacatcat caatatataa tgaagatgta tgcccaaatc caccatcttc tactaatttt
    48241 tctgcttttc taactgcatc atcaaaatca cttgccttgt acattgcaag tactggtgaa
    48301 agtttctcat gagcaaaagc ttcttctatt tcaactgatt ctacttcacc tattaaaacc
    48361 ttagtatcta ctgggacttc aaagcctgca agttttgcta tagtacaagc tgtttgccca
    48421 actattttag aatttaatcc tccatttact aaaattatat ttcttatttt ttctgtttca
    48481 tctttatcta ataaataagc accacgctct ttaaattctt ttttgacttc attgtatata
    48541 ttttctaata ctataaccga ttgttcagaa gcacatatca taccattgtc aaaagtctta
    48601 gatacaagaa ttgagtttac agcagttttt atatctgcac tatcatcaat tattgctgga
    48661 gtattacctg caccaactcc aatagctggt tttccagatg aatatgctga tttaaccatt
    48721 ccaggacctc ctgtagcaag ggtcaaatcc acttctttca taacagttgt agttaattct
    48781 aaagatggct catctatcca tcctattata ccttcaggag ctccagctaa aacagcagcc
    48841 tcaagaacaa cttttgcagc ctctatagta gaattttttg ctcttggatg tggagagaaa
    48901 attattccat ttctagtttt taaagctaaa agtgctttaa agatagcagt agaagttgga
    48961 ttagttgtag gtacaactgc tgcaataaca cctattggtt cagcaatttt agtcatacca
    49021 aatgcttcgt ctttctctat aacaccacaa gttttagttt ctttatatgc attatatata
    49081 tactctgagg cataatgatt ctttataacc ttatcttcag caattcccat accagtttct
    49141 ttttgtgcca ttatagctag aggaactctt tgcttattag ctgccaaaga tgctgctaaa
    49201 aaaattttat ctacctgctc ttgactatat gtagcaaata ttttttgagc ttctctgatt
    49261 tttgctagtt ttttaactaa gctttcaacg ttatttacag tatctaaacc aactgacata
    49321 acttcatttt cttttttatt ttctattatt acttgttctt tatgagccat tttaacaacc
    49381 tcctgagata gttttttatt tattatgtta atttattaac attatagcat ttacttttcc
    49441 tattttcaag ttattttttt aacattttat tttttatcta atataacatc atttctacta
    49501 ataatatcta gctttacaaa tacttaattt aactagatat cactttattt tttacgcaaa
    49561 tcactaatga aatcatctaa aaacttaagg ataacgttat ttttttaacg tttatatttt
    49621 tgtatttttt gcaaataaaa ctttataaaa agaattttta atatgatact ctatcaagtt
    49681 taaaaataaa aatccttgaa aatgaaaatt aaaacttaat taacaagtta aagtaaatat
    49741 agttaaatgt acaaacattt acaacatata gtataattaa aaaaatatat attcatgatt
    49801 tttatataat atgaaataaa atatatttaa tattatatag ataaatttga agtttataag
    49861 aaggaggata agcatggaaa aattaaaaac aattagcgtt ttttctttga ttataagtgt
    49921 aattttaacg attggaggta ttggaatagt aacttattat gtagataatt tgtttattag
    49981 aggactatca gtatttgttt taattatgtc ctcttctttt gtatcaacaa cagttagatt
    50041 gatatttgaa gagtcaaaac gatataaatt ctaatttaaa aattggatta agtaaataat
    50101 tttaatatag caaaaattac ggagattagc tcaaaataga ctttttagtc catgaggcat
    50161 ttttttgtat gaaattaaat atattttcat catttcaaca agaaaaagag gcttctctct
    50221 attttgagac agcctctttg ctagtattgt atctaataca taatattata agaaccaatt
    50281 ctattgatgt ggacttttta taacaggctg tagctgtttg ttctctagag caagttctat
    50341 agttggaatt attagctcag tatgtgcaat catagaatta ggctttgctt tgcaattatc
    50401 ttgaccaaaa ggtacaaaat atatattttt agaattaagg agaatgccaa tatttttgaa
    50461 attaaagctt agagcatcat tcgttgatat tgatattact agtggtttat cattacgaag
    50521 atgacctttt gctgccatta atactggtga atctgtaata ccatttgcta attttgcagc
    50581 agtatttcca gtacaaggag ctattattat aatatcagca attcctttag gtccaaatgg
    50641 ctctgcctct tcaatcgtaa ctataggttt atttcctgta agttcatatg cttttttcat
    50701 aaactcttca gaatttccaa atctacaatc gatattttgt gatacatctg aaaatatagt
    50761 gtgtacatta gctccttctt taaccaaatt ctctaactct ataaatattt tatcataagt
    50821 acaaaacgaa ccagtaaatc ctactccaat tgtaagtcct tcaagtctca tttctaatcc
    50881 cttctttcat ttataatatt ttctatagcg ttaacaagaa tttcaccaga tgtctttggt
    50941 gaatatttac caggaagccc aaggcaaaga gtcgcattaa tccctgcttc tttaacaaca
    51001 gaataatcta ctcctccagg agaagaagct atatctataa tagttacttc ttgtgatact
    51061 ttttctaacc tttctttcgt taaaatacac tctggtatgg tgttaaaaat ataatcaaat
    51121 tgtaaaagtt tttcatcaag ttgacttaaa gaaatactgg aatatccaaa tgcattagcc
    51181 gtacttcttg catcattatt tcttgcgcca acacatacat ttgcatccaa agcgtataat
    51241 ttttttgcaa gtatttgtgc acatcttcca taacctaata ctaaacagtt actttgatgt
    51301 atattgatta tacttctttt aatagcttct gctatagccc cttcagctgt agcaactgca
    51361 tttaaaatag caacatcatt cattttcata aaatcataaa tctgaataga gtgttgctcg
    51421 caaaatttac aaactttagt tggtatgtta cctccaaata atatttgatt aggagataaa
    51481 ttaattagca agttatcaat agtagcatca agacaatcat ccgtattttc aatattaact
    51541 tgattctttg taaatggaat tggacataaa ataatggaac ttttggatag tgcttcagct
    51601 agtgaagttg attttccaac gattccattt ataatttcat ttgatatccc ataagtgact
    51661 acagaatacc ctttattaat tagttttttt accatgtaca cttgtcgcag gtctccccca
    51721 ataacagtta tatcgtattg atttgacata ttgtaagcct cctttactat atacaatatg
    51781 tttttatcaa tacattggtt cactaaaatt actaaatatt atacttataa catttttaat
    51841 aatgctaatc tatctatctt ttcagaagag gtatatggaa atgaatttaa aaagataatt
    51901 ttctttggga tttcattttt cattagatta tctttcattt taatcaacaa atctttcttc
    51961 gtaattttgt ctatagttat tacaaataat acaatttgat ttcctcttat agtgtctgag
    52021 tacggcaata caattgcatc ttcaatttga ggaatttttt taacttcatt ttcaatctta
    52081 gtagaactaa ctttaaatcc tccaatattt ataatgtcat cacttcttcc ttcaaatatt
    52141 aagtatccat cattatcata atatccaata tcattgacag aataaggttt ggttgcatta
    52201 tatactgcat attctgtatt cacaaaaatt tttccttctc taatatatat atctattcct
    52261 ggaaatggtc tgcctacact tagaggtttt tcaattagtt catcatagca taaatatgta
    52321 atataattta attcacttgc cccatagtat aaaataattt cagactttgg catatatttt
    52381 tttagatttc ttgcagtatc ttcaaataat aattgagacc ctgtaaaaat acttacaact
    52441 ttgagtactg gttcttttaa gtgtttaacc aaaagttgaa gttttgtagg tataaggtat
    52501 atgttagtta cattatattg ttttattgtc cttatccatg atttacaatt aagacctgaa
    52561 cttattacaa tactacctcc ttcatataat actgacatta tagaatttag attccctgta
    52621 aaactaagtg ttccattaac aaacaagatt gattccccag agatattaaa aataccattt
    52681 tgtataggaa aaaatccagc ccaactctca tatgttctat ataatacctt tggaacactt
    52741 gtagaacctg aagataaagc tcccatacaa atacttttat ctaaataatt tataagcttc
    52801 ttatattgat acatatatat attaaattta ggattaaaaa tagaaaaatt aacttttttt
    52861 gctacttcat tattgttatc atcatataaa tcaataacgt ccatagattc atcagatata
    52921 atgattgaaa tattattttt taataaaata tcatctaata cttttttaga aagattataa
    52981 tgacaaataa taggaatatt ttttgaataa ttaatagcaa aaaaactaat caactgaaac
    53041 attatatttt tagaatatat taaaattggg atattttcac ctaaagaaat ttgctttcct
    53101 agttcctcac tataactcaa tatattttca taagtatatt tttctccatc tataattaaa
    53161 aaatttttat ttttatgcct ctcttttttt attcttaaaa gttcataata gttcataatc
    53221 tacacccttt caagcagtaa agctgaacct agaccaccag ctgcagctat agagcaaatt
    53281 ccatatctac ccttagtctt ttctaatgct tttaaaagat gtaatgcaat aattgcacct
    53341 gaagcaccat atggatgtcc atacgctaaa gctccaccaa atatattata tctatctatt
    53401 aactctggat actttctttg aaacaacaca tctataactg caaatgcttc attaaattca
    53461 acagcagata catctaaata attaaggctt tctatctcta aaagtttatc cattgcttgt
    53521 atcgctgatg ttggacttaa ggttgcatct gttcctatcg tgcatgtgtt aataattttt
    53581 gctttaggct tcttttttac atgttctaaa tacttcttag aacatattat aataaatgaa
    53641 gctccatcat taataagaca tgaatttgct gcatttgtaa tagtttcttt tcctaaaatt
    53701 gatggcatcc tatctaataa cctttgactc atcttatcac gtataccttc atcataggta
    53761 ctattattta taggagatat aatatcttca agtatttttt cctctcttgc ttcttttgct
    53821 cttttatgac tttcctttac ccaaaaatcc aagtctgctt tctcaatttg atataactct
    53881 gctactcttt ccgcaccttc gagcatacta ttttggctat tatcatcagg agaaaattga
    53941 gcaactgtat aatttggatt atttgtatta tatcgcttat cattcttatg atatgtgcgc
    54001 attggttgca gagaactact ttcaaaacca cctgcaataa ttaaatcaca ctgaccagat
    54061 ttaacctttg aaaaagctat gtctatactc atcatagcag aagcacactg catatccaca
    54121 gtaaaagcag gtacttcatt tgacactcct gctgttaaag tcattaatct agctatattc
    54181 cctcctgtcc caactgcatt tccacatata atctcatcaa ttttgtcaat ttcatacttt
    54241 ttaattaaat cttttaaaac acttgctcct agtaattcag gttgaacaaa ttgaaaaatt
    54301 ccatttttta gacctatatg acttctcaat cctcctaata taaaaacttc cttcatacat
    54361 tacacccttc cttatattat agatttatca tagatttctt ctgagtacat tgttcagttt
    54421 agtgcctaaa aaagatgcaa taactgactt tattgtatct ccaattaaaa atggtactac
    54481 tgctgcaaaa agtgctgctt ctactgtcat ttgattaaaa tagcacataa atactgttcc
    54541 cataaaatat ataataggca ttccaacaaa aattgttatt aatatatatc tcttaatatc
    54601 attatttcgt ccttttaata aactaataat tagtgctgca aacaaaaacc caaaataaaa
    54661 accacctgtt gggcttaata actttccaat accagatgta cctccaccaa atactggaat
    54721 cccaattaaa cctatcatta tatatacacc aactgtacta aatgcttgtc ttggtgttaa
    54781 aatcaaagct attaaattta tcattattgt ttgtgctgtt actcctgctg gagtaaatgg
    54841 caatggtata tatatgtaag atgatataca caataatgct acacatattg acattttagt
    54901 taaatcttga atatttactt tagtattttt catttttata attcccttca tatgtcttat
    54961 ttcatttagt ttatatgtac atatacaaag cttctattaa agctttgttt tagtataaaa
    55021 taaagacgtt tttttgtcaa ctattataat tatttaggtt tacatttaat ttataagtaa
    55081 actttatact gttactttat attttaatag tcatttataa aactttgctt
//
LOCUS       DAELTA010000007        51970 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.007, whole genome shotgun sequence.
ACCESSION   DAELTA010000007 DAELTA010000000
VERSION     DAELTA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.007"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(97..2160)
                     /locus_tag="KPG13_000359"
     CDS             complement(97..2160)
                     /locus_tag="KPG13_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG0984107.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2541..5876)
                     /locus_tag="KPG13_000360"
     CDS             complement(2541..5876)
                     /locus_tag="KPG13_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0984108.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5926..6354)
                     /locus_tag="KPG13_000361"
     CDS             complement(5926..6354)
                     /locus_tag="KPG13_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984109.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6344..6724)
                     /locus_tag="KPG13_000362"
     CDS             complement(6344..6724)
                     /locus_tag="KPG13_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG0984110.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6757..7929)
                     /locus_tag="KPG13_000363"
     CDS             complement(6757..7929)
                     /locus_tag="KPG13_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG0984111.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7933..9396)
                     /locus_tag="KPG13_000364"
     CDS             complement(7933..9396)
                     /locus_tag="KPG13_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG0984112.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9906..10775)
                     /gene="gatY"
                     /locus_tag="KPG13_000365"
     CDS             complement(9906..10775)
                     /gene="gatY"
                     /locus_tag="KPG13_000365"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0984113.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10795..12711)
                     /locus_tag="KPG13_000366"
     CDS             complement(10795..12711)
                     /locus_tag="KPG13_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG0984114.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12714..13634)
                     /locus_tag="KPG13_000367"
     CDS             complement(12714..13634)
                     /locus_tag="KPG13_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG0984115.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13736..14488)
                     /locus_tag="KPG13_000368"
     CDS             complement(13736..14488)
                     /locus_tag="KPG13_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0984116.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14880..16325)
                     /locus_tag="KPG13_000369"
     CDS             complement(14880..16325)
                     /locus_tag="KPG13_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0984117.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16347..16700)
                     /locus_tag="KPG13_000370"
     CDS             complement(16347..16700)
                     /locus_tag="KPG13_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0984118.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16729..17976)
                     /locus_tag="KPG13_000371"
     CDS             complement(16729..17976)
                     /locus_tag="KPG13_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0984119.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(17984..18301)
                     /locus_tag="KPG13_000372"
     CDS             complement(17984..18301)
                     /locus_tag="KPG13_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984120.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18543..20456
                     /locus_tag="KPG13_000373"
     CDS             18543..20456
                     /locus_tag="KPG13_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0984121.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20579..21733)
                     /locus_tag="KPG13_000374"
     CDS             complement(20579..21733)
                     /locus_tag="KPG13_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG0984122.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(22012..24627)
                     /gene="mngB"
                     /locus_tag="KPG13_000375"
     CDS             complement(22012..24627)
                     /gene="mngB"
                     /locus_tag="KPG13_000375"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0984123.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24771..26696)
                     /gene="mngA"
                     /locus_tag="KPG13_000376"
     CDS             complement(24771..26696)
                     /gene="mngA"
                     /locus_tag="KPG13_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG0984124.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26826..27551)
                     /locus_tag="KPG13_000377"
     CDS             complement(26826..27551)
                     /locus_tag="KPG13_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0984125.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27814..28593)
                     /locus_tag="KPG13_000378"
     CDS             complement(27814..28593)
                     /locus_tag="KPG13_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG0984126.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28586..30013)
                     /locus_tag="KPG13_000379"
     CDS             complement(28586..30013)
                     /locus_tag="KPG13_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0984127.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            30404..31126
                     /locus_tag="KPG13_000380"
     CDS             30404..31126
                     /locus_tag="KPG13_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984128.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31323..32990
                     /locus_tag="KPG13_000381"
     CDS             31323..32990
                     /locus_tag="KPG13_000381"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG0984129.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(33086..34405)
                     /locus_tag="KPG13_000382"
     CDS             complement(33086..34405)
                     /locus_tag="KPG13_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0984130.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34446..35192)
                     /locus_tag="KPG13_000383"
     CDS             complement(34446..35192)
                     /locus_tag="KPG13_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0984131.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35436..37445)
                     /locus_tag="KPG13_000384"
     CDS             complement(35436..37445)
                     /locus_tag="KPG13_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984132.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37883..39316)
                     /locus_tag="KPG13_000385"
     CDS             complement(37883..39316)
                     /locus_tag="KPG13_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0984133.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39403..41319)
                     /locus_tag="KPG13_000386"
     CDS             complement(39403..41319)
                     /locus_tag="KPG13_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0984134.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41393..42253)
                     /locus_tag="KPG13_000387"
     CDS             complement(41393..42253)
                     /locus_tag="KPG13_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0984135.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42604..43791)
                     /locus_tag="KPG13_000388"
     CDS             complement(42604..43791)
                     /locus_tag="KPG13_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0984136.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43805..45196)
                     /locus_tag="KPG13_000389"
     CDS             complement(43805..45196)
                     /locus_tag="KPG13_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG0984137.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(45791..47491)
                     /locus_tag="KPG13_000390"
     CDS             complement(45791..47491)
                     /locus_tag="KPG13_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0984138.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(47463..48866)
                     /locus_tag="KPG13_000391"
     CDS             complement(47463..48866)
                     /locus_tag="KPG13_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0984139.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVLPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49130..49621)
                     /locus_tag="KPG13_000392"
     CDS             complement(49130..49621)
                     /locus_tag="KPG13_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0984140.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49647..50459)
                     /locus_tag="KPG13_000393"
     CDS             complement(49647..50459)
                     /locus_tag="KPG13_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0984141.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50472..51431)
                     /locus_tag="KPG13_000394"
                     /pseudo
     CDS             complement(50472..51431)
                     /locus_tag="KPG13_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51549..>51970)
                     /locus_tag="KPG13_000395"
     CDS             complement(51549..>51970)
                     /locus_tag="KPG13_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0984142.1"
                     /translation="RLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMI
                     KNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDL
                     NIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        16609 a         9543 c         5496 g        20322 t
ORIGIN      
        1 cttgatataa atttgagcta taaatctata tcaagaggtg ctattttatt tttaatataa
       61 ttaatattct attacgcaaa aaatgcatat atttcattat tttatggaaa atattacttc
      121 atttaactct atatcaaact cattaagtaa aaatctaatt atatttaatt tagcctctgt
      181 atttaaattt gtttctaaat atatattttc tgatatttta ataccttttc ttaatttata
      241 tgcatctcta gacttaatat tatctataaa atctctttta taagttttgt ctacaatact
      301 gttaaacctt acagaatcca aatcatataa agtaagagca acttgttcat aaaattctgt
      361 ccaattttta gccttatatg actctcctaa tatactaaat gatactatac tttcatttgt
      421 aaaattaaca tcatcatcct ctaacgtaaa tatattttca ctgttacttg gaggttcata
      481 tgtaacactt ggatatttcc aaatttctac agctctatct agtaatatct tagttctatt
      541 ttttatttct tcttcattcc atttatctat agatgaaata tatttattta gataaagcct
      601 actatcttta aatccatctt ccatttcttt cttttcttca aatgttttat tacttagttt
      661 tgaattatat cctgtcaaag ttaaatttcc aattgtatgt aaatatttat catgtatctc
      721 cttatagtta tctcctagtg attccttcca tttatttgtt aatgtctgtg gcattatatg
      781 ctctatattt agcatattat tattgattaa attttctaaa tcaacaattt ctttattact
      841 ataattctct aacctctcaa gtatatataa cttattcttt gacttagtat tatatatatc
      901 tttagtttca aaagcatttg aaaattcact gtcagtagga aatctttgag atgatttctt
      961 atttataaaa atatatctta atatattaga atagttttct ttataatcac taaatttttt
     1021 aatttccctt cctaagttca taaatacttt attcagtgca ttcggaggta catcacatac
     1081 tattcttctt aatataaaac attctactaa attaagggat tctacaagta cttttttatt
     1141 tattatacct tcattataat catcaaataa ttctaatata aacggatatg atacaaatat
     1201 ttcaagtttg tttatccttt tcaaaacttt gtttacatca ttatcatcag tacatgaata
     1261 agatatctct ctataatagt tagaaaattt aagtaaatct tttaataaaa tttctatatc
     1321 tataccatta tctgatacat accttttaaa actaaaatat actttatttt tattcggtat
     1381 acttctctcc tttatagtca aatagtctct tataaaacta cttacgttgc tacttgtatt
     1441 tttttcaatt ttataccaat aattattata aaattcctct tgtaacttag agcttctacc
     1501 cattaacaca aagtttctta ctctatcagc ttcacttaaa tctaagccag ttgaatttag
     1561 actttcaaag attaattgag ggtcatcttc accatttttt aactctattt ctactatagt
     1621 taatttctgt atagcatcaa acaaatcatc tattgatata ttatcttctt ttattctatt
     1681 ataaaaatat aaataatttg aaactatatt agaatttcta tctgtattca tttcttcttc
     1741 aaatagttca tagtacgctt ttctatcttc tgctattggt tttaacttta tttttccatc
     1801 tttatctctt gagtgcttgt ttataagata acactcctta atctgttctt ctgttgtaat
     1861 aattcctttt gattccccaa actcttttat tatttgacac atggcaatca ataatattga
     1921 cacagtagtc actctttgtt gtccatctat tattaaatat tcttttccca tactatatat
     1981 agatacaact gaccctaaga aatgagttct aaatccagaa tctattatat cctctaaatc
     2041 atcaaacaat cttttacatt gttctttctt ccagttataa tttctttgat atacaggtac
     2101 tacaaaactt ttgtttgacc cctctaaata actaagaaat tcaatttctc ctgctttcat
     2161 attaactccc cctataagtt aaataaattc cattcataac tccatgtatc tatatattat
     2221 atcaaaattt tgactcataa atgtatatga aagccttatt atataccttt ttatacctgt
     2281 ttttatttta ttaaagtaaa tacatgatta ttctataaaa tatacaaata cttaatttta
     2341 aaaattatta tttaatcttc ttcatgagta attcatagtt atttatcaat taaaaagaga
     2401 tattgcataa ttattcaata cctctctttg tatatttaca ttatccatat ctagttatct
     2461 atttcaaccc aatattagac tacctactaa taaatttatt ctctacatca ttaacaataa
     2521 ttttgcacta aataacatca ttagcctatc tctgatgcat tttctttaat tttatttata
     2581 gttttaatca atttatttct ctcttcaata tgattttcaa ataaatcaat tatacttcca
     2641 acagttctaa atgggtcttc catcaacact ttgttatcct ctataaatcc attcttgact
     2701 acataatcga ctataagttt aacaaaatgt atttgcttag tattaaagct cttattattt
     2761 aaaaactctg aaaaagcttc atttgcagct tctctatcca atcccactag tcttctcact
     2821 aattgactta ctggagtatc tccaaactcc ttcttataat catcacaatt acctaactgt
     2881 tttaagagta tttcctccaa cgtttcaaca tcttgctttg ttatcttttt attatttctt
     2941 aatttaaata ttgctaattc atctttatgg ctatttaaat aatactctac cttcttttta
     3001 taattcttaa gattgtttgc attatataca gaatcatttc tatcttctgc tacaatcata
     3061 tcttcaaaac ttgtataata tatcttttga ctagactttt caagatactt aataagctct
     3121 cttaatgata tacgtacttc ttctacctca aatatatctg aatcttccca aaaattctct
     3181 gtcattgctt tttcaatagt atatttttgt tgctgaatct gtggtatagt cccaagtttt
     3241 gaaagttttt ctgctgtagc cattacatgc cttatacctt tacttgcatt attattttgt
     3301 aagtatgcaa gttgtatatt atacataact acatcaaatc ttttagccaa ttcatcatca
     3361 ctatatgaag ttatgagtgg tgatatatat tctcttatct cattttgtgc tactgctcca
     3421 atattgctcc aaacattttc atttttatac ttatgtacat aacttaaatt catcctaact
     3481 atataactat cctcatttaa agcttttaca gaagttataa gttcttctaa caattctttt
     3541 ctaagttctg catactcacc ttctatatat ttaatatctt gaagttcttt aaccatactt
     3601 acctttatat taaataattt ttcagtaagt gtttctgctt tatttccctc aaatccttta
     3661 ggattaaatt taaaaaattc aaaattatta caaaaatcaa atattaagaa tctctcctta
     3721 tccatatcaa cacctaataa atctggacat aatctagttc ctctacctat catttgccaa
     3781 aacttagtct tacttctcac tttcttgaaa aaaacaagat ttagtatttc aggtatgtca
     3841 attcctgtat caagcatgtc tactgaaact gctatttgag gtaatttatt cttatcagaa
     3901 aaatcatcta ttatagaatc tacataattt atactatagt caactgcttt tacaaagttc
     3961 ccaccatact ttggatacag cttattaaat ctttcaacta ttgccctagc atgctttgta
     4021 ttctttgcaa aaattatagt cttaccaatc ttttcttctc cttctatttt caacccctct
     4081 gtcattagct tatttaaaac taaatctatt gtgtcagaat taaatagcca ctcatttact
     4141 gcattattcc ctatttcgtc tccaatactc tcatcatcat taaatgtatt ttcatattct
     4201 tccttctctt catcagaaag ctcatcatac ttaattccat cttccataat cttagtctta
     4261 aactcaatac tagtataatc tactaaatat ccatcctcga ctgccttatt atactcataa
     4321 gcatatgtag gtactccatt ttccatgtca aaaactgaat aagtattttt atctatctca
     4381 tcctttggtg tagcagtaag acccattaag taagaatcaa aataatcaaa gattgactta
     4441 tactttttat aaatacttct atgcgattca tctattataa ttaaatcaaa atgaccaggt
     4501 gtaaacaatc tctttccatc cttagacttc gtatcatcaa ttgcattcat cattgttgga
     4561 tatgtagaaa atatcatcct agcatcttct gggctgtctt tgctatccaa taaattacat
     4621 aacgccaagt taggtaatag cttagtaaaa ctttttttag cttgctttac caatgctttt
     4681 ctatctgcta gaaataatat atttttaatc caattatgtc ttgaaagtac atcaactata
     4741 gatatagcaa ttcttgtttt tcctgtacca gtagcacata ccaaaagagt tttgcgttgc
     4801 ttattttcaa gggcctcaca tacagcccga atagcttctt tttgataata tctattggat
     4861 atttcatcat ttattgttac agaacttaaa gacttcttac tctttcttct atccatcata
     4921 agttgtaatt ctgatttttt ataaaatcca taaacttttc tatctgaata gtcatcccaa
     4981 atatacatat caaaaccatt agtcaaaaat attactggtc tttgactata tctcttttcc
     5041 aaacaatctg catataattt agcttgttgt tgaccaattt ttgggtcttt gctcgttctt
     5101 ttcgcctcaa ctactgctaa tggcttacca ttttcaccat aaagaacata atctacatat
     5161 cctacaccac tttcattagg cataccttgt acttctattt cctcccctat atcttttcca
     5221 aattcccaac ctgcaagctt taattctata tttatatatc tttttcttgt ctcaaattcg
     5281 cttacttcgt ctatcttgaa atcatagttt acagtattaa cttttctctt ttcagttagt
     5341 gactttctta attcctcatt ttcttttatt acttcttcaa gctttttatc ttttgaactt
     5401 aatctctcgt aaagattttt aagttcgtct ggtctcgtcc ttttctcttc tccatgaagt
     5461 aataaatctt cattaaagtc ttctgctgta tattcatctg aataacaata gtctatccaa
     5521 ctaacaaatt gatgcaaatt gttaagtgaa agtattgctt ctcctctctc tatgttcgca
     5581 ctagtatgta cagataaatt acctaactta actatatatt ttaataaagg taacatatca
     5641 tagtctataa gttctataaa actattatta tgaatcaacg tactgagatt atcttgataa
     5701 ggcaatacta aatcactatc attagcatat agccatttta ctgcaagctc taaagctctt
     5761 cttgttatag ttgcacaagt agatggacta actagtatac ttttttctgc ttctaagcaa
     5821 gcagtattaa aactattgaa atttttcttc ttttgtaaaa aatcaaaatt acccaaggct
     5881 tttatccccc ttttgtccct tagttaaaat aaatctctct aaaaactatt tcttacagct
     5941 gtttcttata ctaacaaata cttcttcatt ttctgtaaac aattcgtaca tatcttcaaa
     6001 ctgtggtata tcatatccat gagaatcatc atttctcaat tttctaagtg tttctataac
     6061 tatagaagaa tttttaggta ataaattgtg atactcacaa gctaaaaggt agtcataaaa
     6121 gtttttagat tttatgttta aatcaattga tttataatac aacattgctt ttgttaattc
     6181 tagaaatgat accatatagc ttttaaagcc ttcttttatg tatctagccc ataactcagg
     6241 catttcttcc ttatcatata agattttgaa gttttgcagg tcatacaagc aagaaccaaa
     6301 atccctattt acttcttcta ttgagttcca agataatttg tttctaatta ccatacatgt
     6361 ccctccaatc taagatatct attacatcac tgttttcaaa atagaattta tttatataat
     6421 ccttaaaatc tttagattca taaataactt caccttctaa aatagctttt ttaagtgcta
     6481 ttgtataatt atctgtaaca atttttaggt ctacatcaat gtctaatata tttcttagag
     6541 cttctgtctt agttgcaagt tctgtaattg gtactttttt gatgggaatc caagctatgt
     6601 ctatatcact ttctgatgta tgtttatctg ttccataact accaaatatt atgattgcta
     6661 gagtattttc ttttggaaat acatttatta ttgagttttt aatctcattt ggtatatttc
     6721 tcatttaatc acctcttgtt tattaggtaa tattgtttag ttaaataatt ctcctttgaa
     6781 agctttttgc attaatgaat taaagttatc ttctaactct tttaagttgg tttctatttt
     6841 taattttaat ttatccactt gcttaacaaa ctcagaaaac tgattttgaa attcaattgg
     6901 aggaaccatt attaaatatt cacctaactt actaactgat aactcaattt gattcgttga
     6961 accattaaca cattttctat atattgtgtc ttgtatattt gtaaattcaa ataacgctgc
     7021 aaaataatct gaatttaagt tatttgaaat acttcttaaa actgtaacat gactatctgc
     7081 tatatattca gaattatcaa ttttgttaaa ttttacacat cttcctaatg tcccagttcc
     7141 agtagaattt attaatatat cattttgttt caaaaatata tcttctatct tctcttttaa
     7201 gttttcatca taatatttac aattttctac cttcaaattt ctccagtata tacatttttg
     7261 atttataact tttatatttg attgctctac atatttagga gatacacctc tttttaaata
     7321 tatagaaatc tctttaatct tcttcatatc aaattttttt gtattattaa ttggctcccc
     7381 aaacatctca ataaatcttg atttaactaa ttcatccaat gcttctattt gctcttttct
     7441 tttatttatt aactcttgag ctttatttaa aacttctacc attttatttt gtatacaaat
     7501 tgtgaaatat ggtattttta tatcatttaa cttgctttta tttaaagttg cacccataac
     7561 tgctctatca gtattattaa aatttaaagt tctcaaagca taatataaat attcagatgt
     7621 tataagttca ttatttttta tttgaaagtt agcaattgct tcatttgtaa acatgtcttc
     7681 ttttaatatg gcaactctac ctatagacaa cttaaaactc attacaacgg tatttttagg
     7741 tactaatttc atattagctt tttcaacagc taaatctgtt atctcttctt ttgattttga
     7801 tatatatttt tctttcaaat cagaaattga taaccatcta ttaccacttc cccaataatc
     7861 agaagtattt ctagatggtg ttttgccaat atttatatag cacaattcat ttaattttat
     7921 atactccata ccctatacct ctaacattct ttctaattct tctaatccaa cagctatttc
     7981 cttctctaac tccctaactc tacctataat cacactagga gcatcataaa ctacttcctc
     8041 atacactacc tctttatact tattaataga taaatcatac ccattctctc ttatctcttc
     8101 cacatcaaca aaaaagcttt gttctgttct ttttctatct acttccccat caagattatc
     8161 aaatctctta ataatatcct ctatatcact atcctttatc tcatttctct tatcatccaa
     8221 agaataacca tcagctttca tatcatagaa ccatacctta tcagttccac ctgtccctgt
     8281 tttagtaaat atcattatag ctgtagatac tcctgcataa ggcttaaaca ctccacttgg
     8341 cattgatatt atagcttcca atttatgatt atcaactatt tcttttctta tcgatttatg
     8401 tccacctgtt gaaccaaaca atactccatc tggcactata catgcgcatc ttccaccaat
     8461 ttttaaaact cttaaaaaaa gtgccaagaa taataactct gttttctttg tcttagtcac
     8521 ttttaataaa tctgctgaca ctgcttcata atctaaactt cccttaaaag gaggatttgc
     8581 taacaccaat gtaaacttat ctttatctgt attaacctcc gataaagaat ccttatactc
     8641 tatattagga ttgtcaacgc catgcaacat catattcata gcacctattc taagcatagt
     8701 tctatccata tcaaatccat agaacatacc attattaaaa tgctccttca gcccttgcac
     8761 taagaaaaga cttgaatgat tatcccttaa gtattgttgt gatgatacta aaaaacctgc
     8821 tgaacctgct gccgggtcta ctataatatc ttctggtgtt ggtttaataa gtcttactat
     8881 catatctatt atgtgtcttg gagtcctaaa ttgaccatta gtacctgctg tagccacttt
     8941 ggataataaa tactcataca aatctccctt tgtatctcta tccttactta attctaaatt
     9001 actaatacca tcaactattt tagataacat aagaggcgta ggaattttaa atattgcatc
     9061 tcccatgtac tttgcataag ctgagtcttt atttccatgt aattttttta tgaaaggaaa
     9121 cacttcattt tgtactattt catacatttt ttctgaattt ccttcattag aaaacttact
     9181 ccatcttaaa tgttgatgtt catcatcaaa tatcctttca aaagaaactc ccaaaagagt
     9241 tgcttctgct tcattttttg tttctacttc atccaatcct ttaataaata aaagatatgt
     9301 aaattgttct ataacttcta atggattagt taatccacca gtccaaaatg tctcccaaat
     9361 cttgtctact ttgctcctta attctcctgt aatcatatat gactttccct ccagtgttca
     9421 taaaataatt taaatatata ttttagtcgt acgccttaat aattagtgtc ttatattaaa
     9481 ttcaaaattt tactacacag ttattaaatt atttttccta tttacatgtt ttagttaaaa
     9541 aactaatgca atactcttat tttatgatta ttatacatcc catacaaaat tattttatca
     9601 tatatccagc atagaagtca tctacctgga taactcttac catattataa ctttttaata
     9661 ttaatttaaa cttagaaaga aattttttat ttaattatat catcttcctt atttcaaatt
     9721 tctcaattac aatctcaatc aaaaaataaa ataagatgct gtctcaaaat agatataatc
     9781 ttctttttta ttttcggaat gatgaaaata tatttaattt catacaaaaa agagtgcctc
     9841 ctgaactaaa aagttcattt tgagacaccc cttaatttaa tcttacttat tctctccctt
     9901 tattattaac acttcactat ctctattgct tttcctacac atccacaaac ttccatccta
     9961 ctcttaacaa aatctttaac tgactctaaa ccttttttac catatttctt agggtcaatt
    10021 acctctgggt cagaattaag cacatcctta accccattgc tataagcaat ccttaattca
    10081 gttgcataat taaccttgca aatccctctt tttatagatt ccttaataac ttcatctggt
    10141 attccagacc caccatgtaa tactaatgga actgatacaa catctctaat ttcagataat
    10201 ctatctaaat caagctttgg ctctccctta tataatccat gtgccgtacc tattgctact
    10261 gccaaagatg atacccctgt tctctctaca aactcttttg cttctaatgg gtctgtatat
    10321 gctcccccat caccaccatc taaatcgtct tccttacctc caactttacc taattcagct
    10381 tcaacaggta tatctgaagg catacatgca tctacaacag atttagatat tgaaatatta
    10441 tcttcgtaac tttcatgaga accatcaatc ataatagaag tatatccaac tctaagtgcc
    10501 tgcatagcta gtccaaagct tgaaccatgg tctaagtgca ttgctactgg aacacttgcc
    10561 ttctctgatg ctacctttac attcgctaaa taataatcta gccctgcata ctttattgtt
    10621 gaaggtgttg tttgcaatat tactggtgat ttaagttctt cagcagcctc tattactgcc
    10681 ataaccatct ccatattttc aacattaaat gcacctactg cataatgtcc ttcttgtgca
    10741 tctaataata tttgtttagt tgtcactaat gccataattt ctcctcctat atgtttatac
    10801 tgatttgatt tcttcgttat ttaattttgc tactatttct ctacttgttt tgctatttaa
    10861 taactcattt aaaaattctt cattcattaa ttttgaagat agttgagata atgtttttaa
    10921 gtgtaaatta tcaccattat ctgtagctgc tatcataaat ataatatgtg ctaagcttcc
    10981 atcttctgat tcaaagtcaa acccttcttt tgatacacct actgctacac gtggtacctt
    11041 tactgcatct gtttttgcat gtggtatcgc tatccccata ccaaaacctg tagatgatat
    11101 ttcttctctt ttaaggattt cttctttaaa cttcttttta tctaaaagaa cactagattt
    11161 atcaagtatt ccaatcattt catcaattac atctgtttta ttttttccct taacatctaa
    11221 aattactgtt tcttcgctat ataaagagat attattagat acgttttccg attctgcact
    11281 ttcattttta tgttcatctt ttgttacatt tgtattttct atagtatttt tctcatttac
    11341 tactttaggt gtaaccatca tataaagtac agcacctact aatgaaccta caaatataca
    11401 ccctacccat aaaagtggct tattgactaa tcctagaacc aaaaaccctc catgtggagc
    11461 tggtacttct attttcatta tataagttaa aattgatgat attgatgatg caatcataag
    11521 tactggcaac acttttaatg gattttttgc tgcaaaaggt attgccccct ctgttatatg
    11581 agtactacct agtatataat tgattaatcc tgctgctcta tcttcttctg taaatctatt
    11641 tttaaataaa gtagatgcta ttgctattac caatggtggt gttatacaag ctgcagatac
    11701 acctgccata aataaataat ttccctgtcc tagaagcata gttccagtta catatgctgc
    11761 tttatttact ggacctccca tatcaaatgc tgacatacat cctattacta ttcccaaaat
    11821 tatagggctt gaattttgta accccgatag aaagttcatc atagcattgt taagtgctga
    11881 acatggccca ccaagtaaca acattgctac acccacaaga cctacgctga caataggaat
    11941 tataaaaatc gatttaagac cttctaattg tcgtggtaat tttcttaaca aatgtacaaa
    12001 tagtttaact aagtaacctg ctaaaaatcc tgatatgatt ccacctaaaa accccgaacc
    12061 tgtatcaaat gatatcattc ctccaacaag tcctgctaac ataccagctc ttcccgatat
    12121 agattgagct ataaatgctg ccaatacagg aaccatcaag ttcatagaat atccaccaac
    12181 agattttaat gtagctgcta tttgattata ttgttctgaa tttgggtcaa atgaataaat
    12241 cccccataaa aatgatatag cagtaagtac tccacctgct actactaaag gtaacatgtg
    12301 agatacacca ttcattaagt ctttatacaa cttttgacca aacgataaat ttccttcttc
    12361 tgcaatttta ctgcttgata agtttccttt tctaatagct acttttccat caatacatct
    12421 ttgtataagt tcattagctt tatgtatacc ttctttgaca ggtacttcta ttactggctt
    12481 tccattgaat ctgtccatat caacattttt atcacaagca actattattg catctgccat
    12541 ttcgatatct tttgctgtta acttattttc tacacctata gctccattcg tttcaacttt
    12601 tatgtcacac cctaattccc tggctgaatt ttttaatgct tcttctgcca taaaagtatg
    12661 agctattcca gttggacaac ctgttacccc tagaatcatt ttctttccca tttttatacc
    12721 acctttctga ctcttatatt ttttatataa tcttccacat tctccaaatt acctatacca
    12781 ttactttcag ccacacttgc tcctgttgct gctgaccttt ttaatgcttc ttcaatatgt
    12841 tctggatttt ccaaccatat acttaaaaaa gcagctaatg cagaatcacc agcacatgca
    12901 gagctgacta ctgtaactgg ttgagcacta gcataataaa ttgattttcc attataaaaa
    12961 taagaacctt tttctcctaa agttagcaat atattttgag caccttttaa atgtagtaat
    13021 ttaagtacat ccttaatatc ttcttcatct cttacaataa ttccaaatat atctttgatt
    13081 tcttcatcat ttggttttat caaatatgga ttatactgta gtaactcctt taattttggt
    13141 gaacttatat cgagtattgt ctttatactt ttatttttac aaattttaaa tatatcctca
    13201 tagtaattat catcaatacc aggaggtaga cttccactta ttgataagta acttatatct
    13261 gctaatgact gtatcttatc tagcatatct agtttttgtt tttcatttac atatgaccca
    13321 gagtttacaa atttaaattc atcacttcca tcatttaaaa atatatttat acgagttgta
    13381 tcttctaccc aagtaggcaa tacgtcaaat cctctctttt cgctttcttc aactatatat
    13441 tttcctgaaa atcctccaaa aaatcctaat atcttagatt ctataccata atgacctagt
    13501 acaaaagtta catttaatcc ctttccatta ggagaatata cagcatttga tgtacgattt
    13561 actaattttc tctttatccc attagtagat atattcatat ctattgcagg atttgtagtt
    13621 aatgtatata tcattgtctt cccccttgct tgtgctgtgt atgttttcat tataataaaa
    13681 aagtctatga aaatcttttc acaaatagaa aatcgatttt catagacttt tcaatttaat
    13741 tttcagaatt attatatctg accatataat ctataattat cctagatata atatacaagg
    13801 gcaatctgga agatacttca tattcagaaa ttgatatatg ttcatgtttg tatcctacaa
    13861 tatataaatc cgaatacttt ataagttttg cactttcact acaacagcaa gttataacct
    13921 tagctccaac taaattggca ttctgtgctg attctacaag ctctatggta tttccattca
    13981 atgaaaatat aattataagt tcgtcttttt ttattgattt agattttatt ttcattatgt
    14041 tggggtcatt tataaaaaca acattaaaat caagtaattg caatttaaga ctaaactctt
    14101 ttgctacata ttcagtaagc cctcttgcta aaatatatat cttactagat ttatttatag
    14161 tttttattat attgattagt gtagttaaag atatttgttc tataactctc tgtgcttcta
    14221 taaaggattt atttattata tcccccatat tttgtatatc atcattttca tcttttactg
    14281 ttaatctata cctaagttca ttgaatccat ttaaaccaca ttttctaatt gctcttgaaa
    14341 cggttgctgg agatgaatat gtttcatgag caatctctac tatagataat tctggtaata
    14401 ttgattcatt ttcatttata aatttaatta tttcaagttc cgtcttcgtt agattttcag
    14461 aattactttt gtttagctca attatcatat gttatctccc cttatataag ttttatttgt
    14521 ctaaatttat cgagtaatat aatatattac ctcttaacta cttattttta tatacaatta
    14581 agatttagtc aatgtctata tatgtaaatt tataatataa taattttttg cttaaagttt
    14641 attatgttat cttatagcat tttaaaataa aaaagaattg tacctctacc aaaatttgga
    14701 tgaagtacaa tagatattcc tcatactgat atgtttatga ttaaaagtat aaatataaat
    14761 ttcgctatga atcatataat taataaatca ttttatttac aattataacc ccccttataa
    14821 aactatatta ataatatata cagtatttat aaggggggtt gtttatattt aaaaactatt
    14881 tacttttctc ttaattcttc tccattagtt cttattacat tttgatacca ataaaaacta
    14941 tcttttttat aacgttcttt acttccattt ccattgttat ctatatccac atatataaat
    15001 ccatatcttt tttccatttc ttgagatgaa caacttacta tatctattgg cgaccacata
    15061 caataagcta atatttcggt tccatcagct atagcttcac ctatttgttc tatatgtttt
    15121 cctaaataat ctattctgta actatcatga acttttccat cttcaaattt atcatgcatt
    15181 ccaaatccat tttcagctat cattattggc ttttggtatc tatcataata ttgagataaa
    15241 gtattgtata atccttttgg gtcaattgcc cacccccaag gatttgcttt taaatatgga
    15301 ttctttatat tgtcagttgg attcatggtg tttttacttg ctgatactgt tgtagaataa
    15361 tagtatgata aagctagaaa atccatagta ttttctttta agattttatc atcaccttcc
    15421 tccataacta tatttatgtt attttctttg aaaaatctct taatatagac tggatactct
    15481 cccctaaaac ttacatcagt gaaaaaatat tgtagcctat ttctttttaa agcaaatact
    15541 acattttcag ggtcacaatc ttctggataa gatgtacaat ctgccatcat catacctatc
    15601 atcatatctg aatttatttc ttttccaagc ttcttagtca aagctgtagc tatgaattga
    15661 tgatgaatag cttgatatct agtctcttct acattatcaa cttggtctac acaaaatgca
    15721 actgaattaa atgattcata atgtattaaa tttatttgat ttataactat ccaatattta
    15781 actttatttt tatatctatt tagtattaat tctgcatatc taacaaagaa atctaccact
    15841 tttctattag accatccttt atattttaat actaaattca aaggagtctc atagtgaagt
    15901 agtgttacaa taggttccat accattatct attacttcat ttataagatt atcataaaac
    15961 tttaatccct cttcattagg ctctaaatca tctccatttg gaaatattct agcccaattt
    16021 attgaaaatc taaaacactt taaccctagc tctttcaaaa gctttaaatc ttctttgtat
    16081 gtatgataaa agtctattcc actacgctta ggataatatc cattattatc tttcatagca
    16141 aactttattc tttctatagt catatcagac tcataaggtt tagatatatc tctatcttta
    16201 tcatacatat gtacatcagc taaacaccaa cctttgccac cttcattcca tgcaccttct
    16261 aattgattag ctgctacagc tcctccccat aaaaaacctt taggaaactt acccaattta
    16321 atcactcctt attttattta tttaaatcat ttactattat ttaccttata ttctatagac
    16381 tttactattc ctactaaaga catagttata tttaactcgc tgaatatggt cataagagta
    16441 tcttgagcat gtgcaaataa cagcgaatgt gctcttttct ctccaccagc ttccccttgt
    16501 ataatcttgg tttgagctct atgtgcttct gtcataaacc cttttgcttt agctagtttt
    16561 aaatcagcat ttctataatc aaacttttcc aaatcattaa gagcatgttt aatttctgtt
    16621 ctagcctcac ctgcacgaag tattatttgc attgctactt ctactaattc ttcatctatt
    16681 atatgttctt caattgtcat atttataatt tcctttcaat ttctaaattt aaacctcttg
    16741 actctctaat tttatctctt ctttctcata agctttaaaa aatggatacc atatcataaa
    16801 tagcaatact atatttattg ctaaaacaat aagacctcta acatcttgat ttaccataaa
    16861 agtagatata ccaaaaggaa cataccataa attaaacacc ttacttggaa ttgtaacaaa
    16921 tcccatactc attgataaat aagtagttat tgatattact actgttccta tccacatagg
    16981 taacattaaa aatgggttcc atgccactgg agctccaaat attactggtt catttatatt
    17041 cattatagaa gggacaatac atgctcttcc tattgctttt attcttccaa ctctggataa
    17101 acacatcatt ataactaatg gtaatgtagc acccaaccct cctaaagtta tccagcctgt
    17161 ttgaataact tcgtaaacat atatattagt tgctactcct cctgatgcta ctgctgctgc
    17221 attagctgca attgcaccaa gttgaattga ataagttata ggagatagta cccacccact
    17281 tattcctaaa gaatatataa atactactaa gaagtttaag agtacaaatc caggtaatgt
    17341 ctgtgctata tctgatactg gtttaaatac tctaactatt atttcaaaca tgtcaaagtt
    17401 gagtccataa attaatcccc agcctacaaa cattatcact gttataggta acatattatc
    17461 aaaccacttg cttacaaaat ccggaagtgc tgaatcttct ccgaaaaatg aaaaagaacc
    17521 aaataaagac attattaatc cagagaataa tcctgcaact agcgatacaa gcattccttc
    17581 tcctccaaac ctgttgaagt taaatatagc tccttcattc gtaatctctg gacgaatcat
    17641 cattaacatt aatccagcac ctgcaagacc agctactaca ctcatacttt caaatttctt
    17701 attcttcatt gtttccatag gcaataaaaa tgctataaat aatcctaaaa gtccaaatgt
    17761 atattgattt attggactta aatcaggtaa aaagcttatg taattcttca agacagaaac
    17821 tatagttact aatgagccta caaataccat tggcaaaact ttcattatag ttgattgaac
    17881 tgattgtacc catatattct tagtcatatt atttacaaca ggagtaaatt tttcacttaa
    17941 aaaatctata aatttattca taaacactct ccccataaat attttatttg tttgatatta
    18001 gttctagtgc ttcttccaaa gcctttgcac catctagtct tccatatgca gttttagata
    18061 tacatgctac tggaacgtta tattcattag ccttttgttg aatttcttcc ttcatataat
    18121 ctaaatgtgg tcctagtaat aaacaatcta tttctgataa atacttatct atctctgact
    18181 cacttctcgc ttttatatct gcttctatac cttttttctt tgcactcttt ctcatactag
    18241 ttgccataaa acctgtactt gctcctgaac cacacgccaa aagcactctt atatttttca
    18301 taataatacc tccaatattt ttatccatta atttatttct taatgaactt tttattttat
    18361 tatgtttata ttttacttcc caaataagtt catttcacat aaatatattt cctaataata
    18421 ggaaatcatt tactcttaat tagacctatt aatatagtat aatgtatctt ataatacgta
    18481 cattcaatta tataattatc atatcataca gattttattt taaaactatg cgagggaatc
    18541 taatgaaaac atctaaaaaa gagctatttt taaatttcat aaaaacgcaa catgattgga
    18601 tagattcatc cacattagct aattacctta atgtatcaac acgttcgatt agaaaatatg
    18661 taaacgagat taattctaat ggtgcattta tattatcttc taaaaagggc tataaagtaa
    18721 atctcaatac taattgtcaa actaagattg atagttcaga gaatatatca cctgataaca
    18781 gattaaattt aattttaaaa gaacttatag ttaatagtaa tggaattaat atatttgatt
    18841 taagtgaaga attatttgtt agcccagcta ctattgaagg ggatatagtc aaagctaata
    18901 aatttatagg aagttataat ttaaagataa agcaatcgaa atttttattg aaactagttg
    18961 gaaatgagtc tgcaaagcgc aagttaatga gctctataat atttaaagaa acgggaagtg
    19021 attttttaag cttatttgat gtacaaaaaa tatatcaaga gtataatttg actaaactaa
    19081 aagaaaatat aatatatata ttaaaaaaat acaatttatt tataaatgag tatgctataa
    19141 acaacatact tcttcattta atgataacta tagacagaat aaaaaagaat aattacatag
    19201 actctgttga agtagtaaac tacatagaca ataacgtaga tataaatatt gcaaaagaca
    19261 tatcaaattt tttagaatct gaatacaata taactttaac tagtgcggaa ttatactatt
    19321 tagtatttca gttaacaaac aaaactacag tcttaaacta caatcaaatg gatactaaat
    19381 cattgtcaaa ttatatagat gaacactttg ttaaattaac taaaaaaata attaaaaatg
    19441 tatatgactt atatcttata gatttatctg atgaggaatt tgttgtaaaa tttactcttc
    19501 atgtaaaaaa tttaatcagt agagccaaaa ataatcaagt tttaagaaat caaatacctc
    19561 aaaagttaaa agattcctac cctttgatat atgatatatc tgtttatatt tgcaatcaga
    19621 ttcaaacttt agaaaatgta gatatagacg aagatgaaat atcttatata tctcttcatg
    19681 taggttcatt ctttgataga caaaaattat tagaagataa agttctatgt gcattaatta
    19741 ctccaaatta ctatgattta caattcaaaa tagtaagaga ccttgagaag agatttaatg
    19801 aatccataga aattattcag attttttcag atactcataa tcttgatttt gacaataagg
    19861 tagacatggt tattactact ttaccaatta acgatagatg ccctatacct tttgtttatg
    19921 taaatcctta cctaaataga aaggattatg ataatattca aagtaaattt attcagatta
    19981 aagatagaaa aaatatactt actgtacaaa atcatttaga aatgtacttt agtgaatctt
    20041 tgtttatgaa aaatatatat ttagatagtg ctaaagatta tataagattt atgggaaata
    20101 ttctgtacga aaataaatat gtcaaaccca attatataga tgatgttctt ataagagaaa
    20161 aaatgtcttc aactgcattt aataacaatg tagcaattcc tcatagcatg aaaatggatg
    20221 ctttaaaaac cggagtatgt ttaattgtta acgataaacc agttaaatgg ggtgaggaaa
    20281 aagttcaaat tatagctatg atacctataa atgaaaaaga aaaagaaaaa ttcaattaca
    20341 tatttgaaag ctttattgaa atattatctg aatggaataa tgttaaagaa cttactaagg
    20401 cagataacta tagtagtttt atgaatagaa tagcttatct tattcaaaat atctaaatcc
    20461 attaatttat ttccaatata tgtttcttaa gttattaaca taaaatagat aagctgtcac
    20521 actaaaaagt ctaaattaat atgtgacagc tttttaccta atataccttg ttttaatact
    20581 acatattttt tgactgttgt atactagtta taagattgaa tatttgtgtt gttgtagctg
    20641 tcatattctc actagcagtt gctttatcca ttgcttcttc tattgtcata gctctattta
    20701 ctatagaaaa ataagcatct ataccctctt cattacattt aactgcatct tctgttgtgg
    20761 ctccagccaa acctattact tttaccccat atttttttgc cagttttgca actcctattg
    20821 gagccttgcc catagcagtt tgattgtcca atctaccttc accagttatg acaaaatctg
    20881 catcttttaa ctcttcctct aatttaattt cttctaatat tatttttatt ccagattcta
    20941 attttgaatc taaaaatgct aaaaaagcaa aacctaatcc tcctgctgct cctgctcctt
    21001 ctacattagc tacatcttta cttaagtaat ttttaactac tttggaaaaa tttctaagcc
    21061 cattatctaa cttctcaatt atttctgatg ttgcaccttt ttgagacccg tatatgtatg
    21121 cagctccatt tttcccaaac agcggattat ttacatcaca ggctatttta aacttacatt
    21181 catctaattc ctttaatcta ttttctattt ttatatgtct tatttcattt aaaacttttc
    21241 cacctaatcc tactaacttg ttatttttat cataaaactc aaaccctaag gcttgaagca
    21301 tacctatccc agcatcatta gtagcacttc cacctatccc aactataaaa tttctatagc
    21361 ctttatttat tgcttccttt attatttccc caacaccata agttgtagtg tataaaggat
    21421 ttctaagttc agtcggaacc aatgttaatc ctgatgcttg agctatttct attattgctg
    21481 tacttgtatt ttttaataat ccatatgttg catccacttt tttagctatt ggtccagtaa
    21541 cttctatagt ctctttttta ccattcatcc cttgaactaa agcatctact gtcccttcac
    21601 caccatcagc aagtggcaat atctttactt gcgcatcttc tttaaccttc aatatcccct
    21661 tttttattgc atttcctgcc tctattgaag ataagcttcc tttcaaagaa tctatagaaa
    21721 ttaatatttt catacttatc tactcctttt tattactcct tattttaaac atataatttt
    21781 ataagcttaa tttatctttt attttatact ctacatctct ctttgtatac aacttatacc
    21841 attaatattc ttttatctta taaaaaggga gtgtctcaaa ataaatttct tagttcatga
    21901 gacactcttt tgtataaaat caaatatatg tttattattt caacaatgat aaggaggatt
    21961 atctctattt taagataatt cctttttagc actatctaat catttttagc actatttaac
    22021 cagtatagtt ttaacttggt tcattaagca ctctacattt acagagttat tatctattga
    22081 tatagattct aattcttttt catttaggtt aatccattta gctgttttaa tatttttgtt
    22141 aaatactatg ttagctgatt ttgtttcttc tttagaatta taaaatctaa ctactaatgc
    22201 ctcttctttt tcagcctttt ttacaacact taacacaagg tccttattag tttccttaag
    22261 taaactatat gaataatcta cttctatttc tgattgattc aatttcatag cattatatgg
    22321 cattttatta taagtttgta tttttgttaa atactcttta gccatatttg caacatttgc
    22381 ttctagtgta gatttttcat gagtaactat tgctaagtct atttttatat ctcctatcat
    22441 ttgagaatct ggagtaggca actttattcc tgatggacga cctgggcgtc ttgccatttc
    22501 ttctttacct aggaatccta cacttctaaa taatgttata gctattgtgt caaatttttc
    22561 ccctactatt tcaaactctc ttgtactgtt tgtaagaaca gctactccat gctctttgtc
    22621 agacaatcca acgaatgtta gcattggata tatagaatct ggtctttcac tccagtcttc
    22681 ctgttgccaa acttccatag catcatcata cacacttctc tttatatgtc caaactgatt
    22741 atcagacaca gaaaactttg aagctatatt tgttggtata taagttctaa ccctacaatc
    22801 tcttgcttgg ttatttatgt tgaatactac atctattata gctttatcat ttggaacatt
    22861 tataactata tcaaagtcaa taaatgaatc tgctctatta ttttttctag cttgtatatc
    22921 ttttggtact tttaatctat aatttatctt tatctcatta ttaaatctat ttttctttat
    22981 agtggaattt gctactatat tatcgttaaa tattaatttc tcatttggaa gtggtgaaaa
    23041 atcatactca tctccttcat ctcctccatt ttccatcaat aatacatttt caaaagtttt
    23101 atttaatttt ttatcaaata tctttaatgt cccgttttca ttaacttgaa tagtataaaa
    23161 gtcattatct accatgtcac atacttcata agctttttct aacatatgtt caacttcttt
    23221 tatcatatac gccttatacc ccattgcagg tattttatct ttaaagttta ttgtatatac
    23281 cataaatgaa tcatagtttc cataatgaac aatttgacgg tctattaatc ctgcatctat
    23341 tacttcttta tgtaccactt caaaatccaa cttattatta tctttatcaa atatttcaaa
    23401 tgctttcatt ttagttataa cttgggcgct cactatttca ttcctatcgt aaggcattaa
    23461 gttaaatgct attaacttat ctaaagccat ttcacaactc attgcatcaa ctattcttct
    23521 tttgtaaaat gttattaatt ggtctacttt ttcctcagct aagaaaaatc tattcattat
    23581 ttcctggtga actttatctg aacaacaaca tccaatgctg tcatgagcat gatttttcat
    23641 tatttctttc caaattaact ctattaatcc atgatgatat tcaaatccta gagaatatgc
    23701 aatagaagct agtggctcca atatgttagt tatctttgat tctattctag catttgctga
    23761 ttttatatcc attcttgttg agaatatact tctatgaacc ctcatatatt tcccatctaa
    23821 aaattcccct tttattgtat ctatatcctc atttttttct agctctttaa atatgttttc
    23881 atatctactt aagaaaaatt ttctgtctgg ataacattct tttaatttat atataacttc
    23941 aaatatattc ttttgaattg gcatttggtc atgtccattt ggaagtatta tatggtcagt
    24001 agttgcaccc ttatcaagaa ccggtaaata tttatccatt cttgttctta gctcatcttc
    24061 attcattggt agatattttc ctattgcata acctaatgga agtatttgaa ccaaaacaga
    24121 agacccatca tcactcttcc aattaaactc tgttttatta gttccttttc tttcagaaca
    24181 tcctctccaa aacattgaat attttatatc aaacccattt agtatttgtg gcatttgagc
    24241 actttgacca aatgaatctg gtaaatatcc tattttcata tattctccaa attcatcaca
    24301 atcttttatt ccatatagta aatttctaag tatggattct ccaccaacta ccatttcatc
    24361 agtttgagta taccaaggac ctattattag ccttccttct tgaactaatt tttttattct
    24421 ctccttacat tcaggcttaa ctgctaaata atcttctaat atagcagttt gaccatccat
    24481 cacataatat ggatagttat gattattttc taacatatcc attatttctt ccatattatt
    24541 tacaagtaat attctagatt cctcagttga gaagtaccat tctctatccc aatgcatatg
    24601 aggaactatg tgtacatttt ttttcattgc ttatcctcca aaacttatct gataatattt
    24661 tctgtctaat taaaaatcca ataaaaatac tactgagttt acaaaatttt ttaatattta
    24721 ttttatcact ataattaaac ctttgattta aatatctaat aacgtatctt ttaatgtatc
    24781 tttctttgat ttttttgttt cctagttatt attaataata ttgctgatat tatagctcca
    24841 agtactgccg caaagaacca tattgatgct ataataaatc ctgacatact tccctctaaa
    24901 agcgccaatg agaatatccc cgctccaggc acttgaagac ctatattgaa gaatgctact
    24961 atagacccag ttatggctga accagctacc aatgatggta taacacggat tgggtcctct
    25021 atcataaatg gtattgcacc ttcagttatt ccagccaatc ctaatatcca agtttgcttt
    25081 cctatttcct tttctgattc tgaaaataaa tctctgccta aaacagtagc tattgtaact
    25141 ccaaaagctg aaaccatctt aactgatgca aatattgcat aaggtataaa atttccactt
    25201 gctattgctc ctattgcaaa tgtataggct gctttattta ttggtccacc taagtcaaat
    25261 gaaaccatag ctcctattat tgcacctagt agtattgcat ttactccagt catcccattt
    25321 aaccaattta taagaccatt gttaagtgct gctacaggct ttcctactac aaataacatc
    25381 aataaaccta ctaccaaaga accaactact ggatataccc aaaaacttac gaatccagct
    25441 aatgtgcctt tggctggtat attcttcttt aaaaatttta ctatataacc agcaagtaaa
    25501 cctcctaaca tacctccaag aaatccacta cctattaaat cagctgctag acctgctgca
    25561 aatcctggtc caagacctgg tttttcagct agtgagtaag acatatatgc tgataatact
    25621 ggaaccatca atgtacctaa catacctcca gaaagctttc ttaattgcca taaccaagaa
    25681 ccttcttgat tgtatagttc ttgtagacca aatgcttgag atgctaatac tgcaaatgca
    25741 agtatcatac ctcctgccac aataagtggt attatatgag atactcctgt aagtattgct
    25801 gattttacat cttttttcca tgatgatttt tcacaatatg atatatcaga tgatttttct
    25861 atataatcac ctttaccttg tttttgtgct acttctaaag cttcgtatat tatttctttt
    25921 gctcttttta gtggagctgc tactgacgtt tttatgtttg gcaaatgttc aaatctttct
    25981 gtatttttag gtgctacatc tgttgcatat attattgcat ctgccctttt tattatatct
    26041 ggcgtatgag ggtccaccat accattagct ccttgctttt ctacatacac atccacacct
    26101 atttctttac cagctttaat taatgcttct gctgacatat atgtatgtgc tataccggca
    26161 gggcatgctg ttattgctaa tataattttt tttgattcat tttgaatcct aatctcttct
    26221 tttatttgtt ctttcttttc ttttatatct aagttattaa ataactcttt cttagttttt
    26281 gaatttaaca acatattcat ataatcttca tttgacagtc tagtaactaa ctcagaaagt
    26341 atttgtatgt gtgtatttcc accttcactt tctggtactg ctattaaaaa tattagctct
    26401 actttattat ttgggtctac gctttcccaa ttttctattg gtgtctctaa ggtcatagct
    26461 gcaaatgcag cttctttaac tgacttagat tttccatgag gaattgcaag ccctctttca
    26521 aaacctgttg gagataaagt ttctctgtac attacagctt ctaaatattc ctcataagat
    26581 gttaacttgc cttcatcata tagctttctt gctaagaact ttattgcgtc ttcctttgtt
    26641 tttatttttt gttttaaaaa aacaaggttt tcatttgtga ttttcttaaa ttccaaaatt
    26701 acgccctcct taaatcattt aatgctatga atatagcata aaataaatta tgaaaatgtt
    26761 tttttgaaat tatacacaca atttcttctt aaaaaataaa gatttgtgta tttatacaca
    26821 aatctctatc ttaaccaata ttgctctatt attgctctca taatcatcat aattggaacc
    26881 ttatctgata tattgtaccc atttacttgt ttaattgttg aatagcaata taaacttata
    26941 tctgcaattt gttgcaattg attttcacat atatgtgtta aagctactac cttacaaccc
    27001 ttattaactg ctgtctttgc tgcttctata acttctttcg tttctccact taaactaagt
    27061 gcaaaaaaca tatcttgttc acctaatttt cctatatcat gaagtacttc atgtctttga
    27121 ctatagtaag ttatattatc atgcccacct ctaatttctt ttactaatat ctcacataaa
    27181 ggaatgtttt gacctattcc ataaactata atactcctag atttttgaat tatatctgct
    27241 actttattta ttttttcata atctattagc tctaatgtct tttttatatc tgattcaaaa
    27301 ccattttttg gctctttatc ctcatttttt aactcttctt ttaaattatt ttttagttga
    27361 gagaaaccat catactccat ttttttacat agcctaacta tggtatttgg gacagtaaaa
    27421 aatttatctg ctaaagattg tattgataat tttactattt cttctttgtt ttttctcata
    27481 tattcaatta tcatgtcttc tgtatctgta aatttatact catatttttt tgctctttca
    27541 taaaattcca tagtttctcc tccatctatt ttattattca taagtaaaat tcaaaaatat
    27601 tagctaaaat tttcgttaat ataattataa tttaatattt ttaatttatt aatgcatact
    27661 actcttttta ttatacataa aaaatgtgta cttcctatat tgatagagat gattcaagtt
    27721 tttattttga tacatctcta tcaatatttt tttagaaaat acacattttt ttcaaatact
    27781 tatttttaat taaaacttta ggtaatctat tctttatata taattattag acttgaaaat
    27841 atctttaaaa tcaaccaatt caatattatt tctttttaac cacattttaa cttccttatc
    27901 acatagcatt gcatgttcat atactctctc ttgaataaga gaagatgatt ttataagact
    27961 ataatctata aatcctggat gagtaaccat caatgttata tctttatcta aatttatata
    28021 tttttcttca aataatttaa caaaagattt atttaacttg taaaaatcat tctgaggata
    28081 tttacttggc atgctaatac atgaatccaa ataataggga caacttggta taccgtattc
    28141 ttttgcagca tcacacattg ctgatattga tacatcttct tctataccat gtatatcaat
    28201 atactcaggc aatttactca tatacaactt gtaagtttca atttgagcac aaacttcttc
    28261 atatacttct tcataattaa aaacctcttt acctgtcagt agttgcgttc ttcttatttt
    28321 gctagaaaca aattttccac cttcatctaa caaattcttt atcttaatat gttctttgca
    28381 acaaggttct cctattacta aattaacatg taaaccaaaa catacatcat cattttttac
    28441 catttctata gcttgtcttg aaaaaggcat attcaccatg attccagcac ttgtaacaat
    28501 accgttctta taagaagata ttatccctag tgtaacacct tctgaatatc ctatatcatc
    28561 tgccctaact actaatttct tcattttatg catctgcctg ctttctcaac tcatcaagtt
    28621 gcatttccat ctcatccttt actttaacaa catccgttcc tatgatgatt tgaactgatg
    28681 tattactcaa tttaattacc cctttggctc ctgttttttt aatattatca tcattcaaaa
    28741 tagtggcatc cttcacttct aaacgtaatc tcgtcataca attattaaga gtcactatat
    28801 tttcataacc accacaattt ttaattatct gtttagccat atatgaaaaa ttaccatgag
    28861 aaagttttaa attcttttct ttttcactaa ctttatcacc tatctctatc tcaacttcac
    28921 gtcctggtgt ttgcacatta tcttttatta ttatatattt aaatgttaca taatataata
    28981 caaagaatac aactcctaca ggtataacta accacgcttt atctccataa ctaaaactta
    29041 agacatagtc aattatactt gctccattaa atgaacccaa gcgagcaccc aataaatata
    29101 gtacaactcc acctagacca gaataaaaag aatgaattat atataacttt ggagatgcaa
    29161 ataaaaacga aaattctatt ggttcagtta ctgttgccat aaaagaagtc aatgctccag
    29221 cggtcattgc accttttact tcttgcttct ttttatcttg tgctgtttta tatatagcat
    29281 aagaaatagc aggtattgaa aacatcataa ttatgtaaaa tcctgtccag aagaatcctg
    29341 catttgggtc tcctgccata aatctaggaa tttctcctct tataacttct cctgttgatg
    29401 ttgtatattc acccatttca aataatatgt aagcatttaa aacttgatgt aacccagttg
    29461 gtattagtgc acggttacag aataaaaaca agaatattcc tattgcacca gctcctccca
    29521 accaaaaacc aaattgattc attgcagctt gtggatatat ccataataaa ctaaatatat
    29581 atgataaaat taaagcaacc actggtgata gtgtaattac acatttttct cctgcaaaga
    29641 agcttaaaaa tgctggaact ttaactttat atgttttatt gtaagtccat gctatacaag
    29701 ctccaacaat tatcccacca aatacacctg catttaatgt atcattcaat aactctaaat
    29761 ttgacttaaa tatcatatat gcaactacgg ctgcaaccat tacacttgtc ttgtctttag
    29821 actttgagta ggcactagct gctcctaata aaaataagta gtccacattt gaaaatacta
    29881 tattagatat attagatata gctggcacat tgagcatatt tggaccaaat aataatccta
    29941 atatacctgc tgctggcatt acagctattg gcaccagcat agtctttcct aaaagtgaca
    30001 gttgttgcat cattttgttt aagaatctct tcatatccaa tcccctttct atttcggttt
    30061 aattttttgt gaaaacgatt tattcacatt ttatatttta aatattatat ttgtttaaac
    30121 aaattgtcaa tattttttat tcattttatt tttatcattt agtttaacat ataaatcctt
    30181 ttaagtgtat tgctatatct agtctatttt tttaattcat tttttttatt ttaaaaatat
    30241 gtttaaccat gtatttgaaa caacccacaa aataattcta ttttgtcctt tttatggttg
    30301 atatgattct attttcacta tacatgtcta aacacatttg atataatttg ttcatatcag
    30361 ttcaataata ttactaaaga cctttaagga gggatagggg tatatggcta aaaataaatt
    30421 ttcagagata tatgaagtat tgaaagagga aatacttgat ggtaaatata caagtaatat
    30481 gatgctgcct actgaacttc aacttattga aaggttttca tgtagcagaa acactgtacg
    30541 tagagcaatt tctcaattaa acacagaagg atatgttcaa agtataaaag gcaagggtgt
    30601 tgtagtgtta gaaaatagtt gttctaatga tttttttcta aatatgcaca atttcaaagg
    30661 tgtagaatcg attgtagaag ataaaaaggt taatactgct actagtgttc tccacttcag
    30721 caaaatctta attgataata agttatctaa aaagacaggt tttaaagttg gtagtgaagt
    30781 atattacctt cacagactaa gatatattga taacatacct aaaattctcg acattaacta
    30841 ttttctatgt agtatagtta aagaccttga tgtttctata gcacaaggct ctatttataa
    30901 atatatagaa gagtgtctag gtacaaaaat tgtttcatca agaaagatat ttaaaattga
    30961 aaaagctaca gaacttgagt taaaaacgct acctctaaat gattataatt gtgttggtgt
    31021 tattaaaaat agtgtatata ctgatgatgg aaagcttttt gaatatactg aatcaaagca
    31081 tacacctgaa acatttgtat tcatggatgt tactcaaaga tattaaaaaa attatgttta
    31141 attaaacata atttttttac gtttagacac tttatcccat actaacctca ctattctata
    31201 tacaattata tgtttataca aataattatt tattgtatat tttagcttta atattagttt
    31261 aacgcttgac aatttgttta aacatattat aattaactta taaattacag gaggtatcaa
    31321 gtatgaaaaa ttggtggaaa aaagcaactg tctatcaaat atatccaaaa agtttcaaag
    31381 atagcaacaa tgatggtata ggtgatataa atggtattat agaaaaatta gattatctgt
    31441 attctttagg tgttgattta ttatggttga ctcctatgta cgtttctcct caaagagata
    31501 atggatatga tatagaagat tattataata tagacccaaa atatgggaca atgagtgact
    31561 ttgaaaaact cttaaaagaa gctcataaaa gagacataaa aataatgatg gatatggtcc
    31621 taaaccatac atctactgaa cataaatggt ttaaagaatc taaaaaaagc aaagacaatc
    31681 catatcgtga ttattatttc tggaaagatg ctaaacctga tggttctgtt cctaataact
    31741 ggatatccag atttagtgga actgcttgga aatatgatga aaccactaac caatattatt
    31801 tacatttatt tgaagaaact caagctgatt taaattggga aaatgaaaag gttcgtgaag
    31861 aatgttacaa aatactagaa ttttgggctg ataaaggtat tgatggtttt cgtttagatg
    31921 tagtcaacct actctccaaa acacctggat tacctgatga ccctataaca ggaccaaaag
    31981 gagatggaag aactcactac gctgatggtc ctcgtataca cgaatattta cataatatga
    32041 atcaaaaagt tttcaaacct aaaaatatag ttactgtagg tgaaatgtca tctactacac
    32101 ctgaagagtg tatcaactat actagagaaa atcgagaaga actatctatg gtttttagct
    32161 tccatcatat gaaaacagat tataaaggag gcgcaaaatg gactaatgaa atgttctctc
    32221 tggataaatt aaaaaaagca caatctgact ttcaatataa aatgtatgaa ggaaaaggat
    32281 ggaatgctct attttactct aatcacgacc aacctagagc tttatctaga tttggagatg
    32341 atagattttt tcgtcaagaa agtgctaaga tgttagccat aacattattt ggtctacagg
    32401 gtactacata catatatcaa ggtgaagaaa taggtatgac aaatgcctac tttactaaaa
    32461 tagatgaata tgatgatgct gaaagtaaga atgcatacta tcatatgatt aaatctggta
    32521 ttgatgaaaa ggaggcattg cttatacttc aacaaaaatc cagagataat gcccgtactc
    32581 ctatgcaatg ggataatact atttataagg ggttttctaa tcataaaccg tggttaaaag
    32641 ttaataatga caatattagt gtagaaaatg agcttaatga ttctcgttca gtattttata
    32701 cttatcaaag attaatcaaa tatcgtaaac aatatgatat ttttacagaa ggtacttata
    32761 gattgttaga tgaaaatcat aaaaatctat ttgtttatga aagagagtat aaaaaccaaa
    32821 atcttcttgt tataagtaat tttactcatg ataatgtagt atataaatta cctgaaactt
    32881 atttgaataa attaaactac acaatacttg aaaccaatta cactagagat ataattgaaa
    32941 ataaaatgga aataaaacct tacgaaagta taatgatata ttataaataa agttatttat
    33001 gtaaaaatcg gtataacacc ctggtgttat accaatttta ctaaattcaa agtaatattt
    33061 atagaatatc tatagattac aataattatt tgacttctac atcattatat ttctttgaat
    33121 atactttagt catatagaaa tatataggaa ctacaattat catcccacct aatccccaaa
    33181 ctatcttcat aggttcatca atacctgctt ttatcagtaa taatatactt ataataactg
    33241 caaatattgg tataattgct cctccaggta ttctaaaatt tacatcttta ccagcatact
    33301 tctttctcaa tattagtaca gctaaacatg taggaatata ttgaataaat ctagatacaa
    33361 cacttatttg agataatgtt gtgaatgaac ccgaccatgc tattagtaaa gttcctatga
    33421 cagatactat aattgctata taaggagctc catttttatt agtttttctt atagacttag
    33481 gcattaatcc ttgttcaacc aaagcagcag cagaccttgg tgtaaatatg gatgatgcaa
    33541 tatttatacc tccaacagat actatagttc ctattgtaat tatataccca cctataggac
    33601 ctaacatctt aatggcagca tctgcaacag gagtagctga ccctgctaag ccattaccca
    33661 acacccctat acttattccc agtataagca tatatattac tgatactaca aacattacca
    33721 ttaccaatgc tttaggtaca tttttcttag ggttctccat atcttttgct gcaacagcta
    33781 aagattcaaa tcctgtaaaa gcataaaata aagttataga tactgctaca aaagctgatg
    33841 acgcactttg acctttttgt attattacaa atggggtaaa attagaaccc tttataaaga
    33901 ataatcctac agctataaat aaaactattg gtaataattt tcctatagtc atgatattat
    33961 ttaagtgctt agattgtttc atccccataa aatttataat cccaagtcct acaactaata
    34021 taccaattat tatattttta gtaagtggtt ctgctgcttg tggccataca gatgatagtg
    34081 ttacagcaaa gaaatttgcc atagtagccc aagcaatcat actaactatc cacttcataa
    34141 atccaacttc aaatccaaca aaatctccaa atgcctcttt tgcataaaga tagggccctc
    34201 catcagtatc aaaccaacta cctgcttcag caaaacataa agcaatagaa acagctaaaa
    34261 gcatacaaaa aactaatact aatatacttg ctggacctat atctgtataa gcatcaccag
    34321 gtaacccgaa tatacctgac cctattattg cattgattcc taatagtaca atactccaaa
    34381 aacctaactt aatgcctttt tccattgtta ttccccccaa agccaactta atatatttgt
    34441 attttttatt tagatgcttc tttaactatt gccataaata tttttttcat attatcataa
    34501 tccctagtca tcatttctgg atgccattgt atccctatta caaactcact tccttccttt
    34561 tctatagctt caattaaacc gtctttagat gtagctgaaa caatatatcc cggtgctact
    34621 ttgttgacaa ctaagtgatg aaaactatta actaaaactt ctttttctcc taatatttca
    34681 tataattttg tcccctcctt tatctgtact gtatgtgtag gtacattaga tagatgttgc
    34741 tgattgtgtt taatataagc accttcaatt aaagataaat cttgatacaa actacctcct
    34801 tcagttacat ttataatttg ttctcctcta catataccta aaactggttt tttcatatct
    34861 aaagcatgtt ttaataattt catatcaaaa ctatctctct ttggtgatat tgcccccaac
    34921 ttattatgag gttcttcttt ccatatgagt ggatttacat cttgtccccc agatagtata
    34981 agcgcatcta tattagatac ctgctccttt ataatctcat catcatatac aatcggtaca
    35041 atatatggta tagctttaca cataactact gattgtatat aatcattatt tacataagcc
    35101 ctttcatagc caggaaacat tcctccttca tcaattagta aattacctga tattcctatt
    35161 attggatatt tctttttagc caatttcttc accccttaac ttatttgtga acaataaaaa
    35221 atgctacttc tcttaaagag caagtagcat accaagattt tatttatttg aacattataa
    35281 tttattttag aaatactttt tttgaatacg agtaatataa ataactttat ttacttatat
    35341 tttattttaa tcctgtttag tttgactttt ttaaattgtt ttaagtacaa ctaataggta
    35401 aaataggcat ttagattttc ctttttatca acctattaaa tactgtttaa tatatttttt
    35461 ccccttctat ttaattcact tccctttcta ccagattcac atattatcaa atccagctct
    35521 tttaacatat taagtattcc ccttacctca ccttctgtta tattgtagcc taattcattt
    35581 attgaattaa caataccatt tctacctata tttttattaa gattattggc attttttaaa
    35641 cactccatta ctaactttac tttctctata gatgttctat cctttaatat acttatttct
    35701 ttactaaaat cttcagttat atttaataag ttatgaggta aatcatttat taaaataaca
    35761 tcctcagaac tcataatgtt aatatacata gcagtatttt ttaattctct tatattacct
    35821 ggccattcat atgcattaat aatgtcttga acatcttgac taattgctct cttccttttc
    35881 ataaaatgct ttaacataac ttctatatct tcttttcgct ctcttaaagg tggtatattt
    35941 atgggcaata catttatcct atagtataaa tctgctctaa atctatctcc ttttatcatt
    36001 tctaataaat ttttatttgt agccgcaatt actctaacat ctatatctat tacattttct
    36061 cctccaaccc tcattacttg attctcttgt aatactctaa gtaatttgct ttgtaagtat
    36121 ataggcatgt ctcctatttc atccaaaaat atagttccat tgttagcctg ttcaaataat
    36181 ccttttttac ctccttttaa agctcctgta aaagcacctt cttcatatcc aaaaagttca
    36241 ctttcaagta aattttctgg catagctgca cagtttatcg ctataaatgg ctgactatgt
    36301 cttggtgatg cattatgaat agactgagcc atgagttctt ttcccgttcc actttctccc
    36361 gttataagaa ttgtataatc agattcagct attttcatac ctaatttctt agttctttcc
    36421 atcttcgttg aaactgtttt tatatcatca aaagtatatc tagcaacttg tcccttttct
    36481 ctaagcttct ttgataggtt ttgctccaat tttcttatgt aagttatttc ttgaatacaa
    36541 aaataatatc cagtaaccct tccaaaactc tctatattat acttatttac gtttaagtac
    36601 ttattattaa attttataac ttcatcatgt gccttactca aactaaaaat ctttttaata
    36661 ttctcattct ctaattcatc tatatttttc cctataatat tactttgaat atctagtata
    36721 tcccctaaac tattattgca aataattatg tttcccttat ctgataccat tgccatacct
    36781 aagtttgata aatttattat agaatttaat tcatctgctt ttatagtaag ttctttatat
    36841 ttagtattta ttcctgaata taaactaatt atttcatctg tatacttaat tagcctttca
    36901 gtaatttctt tatcatccag tttgagtttt gctattattt gaataaacgt tgaactatca
    36961 atatacctat ggcctaaatc aataacttct tctatatatt ttggaactaa acttgcctct
    37021 cctggcgtta tagctatctt aatatttcta tattcttcat tgggattgta tggtataaaa
    37081 ttcagatgat ttattcctat attgtataaa ttagatattg tttccaatac agtatgctta
    37141 ttatcgttta ctactaatac atctattcct tctggcaatg aaaataactt atatatatcc
    37201 ttttgcttta tgctcctatt tatagttatt atctttgaga catcttctac atattttttt
    37261 atttttaata ctctatcatc tatcattacc aatattatgt catcttttat taattccttc
    37321 tctgttattt tatttaaata ataatttttt atcattactt tatctttaaa tatcatttct
    37381 agatttttat ttaagaaaca ttctaattta gacaacccat cagttactac tgctattgtt
    37441 ttcatttgat tactcctttt aaaatatact ctactctttt ataatataca tctgattttc
    37501 actttaacag cacaaaaaat tgtacattta gtatactaac atattccttt attagtttta
    37561 tgtccctaaa tatcatagtt gcaatatcta tcatatttct taaacttcta taaagcaaaa
    37621 attgtgccaa attaataatt ttcttaattt tattctaaag ttcctcaata gtatttacag
    37681 acttagcact tatagcttat aagtctttat attattaatc tatttttata ggttaattgt
    37741 acaaatcata tatcactttt acccattaaa tgcattataa aaaagatgga ataacgattc
    37801 gtcactccac cttttttgta ataaaaaatt gtatatataa acactattta ctactcttct
    37861 aagtacaaac attatctaca tactataacc tttcaccatt agattctatt acttgtttat
    37921 accagtcaaa actctttttc ttaatcctag acaagtctcc acttccatca tcatacttat
    37981 ccacgtatat aaacccatat ctcttggcca tctcaccagt agatgcactt actaaatcta
    38041 tacatcccca cggagtatat ccaaataaat caacaccatc ttctacagct tccttcattt
    38101 gctctatatg tgcttttaaa tactctatac gatagtcatc atttatcact ccattagatt
    38161 cttttttatc ataagctcct aaaccatttt caactaccat cataggtatt ctatatctat
    38221 cataaatttc atttagtgca tatctaagcc cttcagggtc tatttgccat ccccattcac
    38281 tagacttaag atatggattt ttcactccac ctaatatgtt gccacttgta ttatcttctt
    38341 tagaatcact acttacacat gtagacatat aataactaaa tgtataaaaa tctacacaac
    38401 cttcttttaa aatttctaaa tctccttttt ccatttttat ttcaatatta ttttctccaa
    38461 aatatctatt catataagtt ggatattcgc cacgcacttg tacatctcca cagaaataat
    38521 tcattactct actatctctt tgagttttaa ctgcatcatc tggattacaa gtataaggat
    38581 acattgttgc aaatatttgc atacatccaa cttgataatt tgaatctatt tcatgagcta
    38641 acttaacagc cttagcactt gctacaaatt gatgatgtaa cccttgaaaa cgaagttgag
    38701 gtatatctac ttggtttata aaatctgtag tcccttcatt taatattcct tgtgataagt
    38761 agcctcccat aggcattgta gctgaattta tttcattaaa tgtcagccaa tattttactt
    38821 ttcctttata tcttttaaat attgtttcac agtaattcat atacaagtct attacatctc
    38881 ttgaaaccca tccattgtat ttctctgtta ttgcaaatgg tatttcatag tgagatattg
    38941 ttattaatgg ttctatatta tattttaaac attcatctat cacattttca taaaatttaa
    39001 gtcctgcttc atttggttct ttctccattc cagttggaaa tattctagtc catgctattg
    39061 aaaatctaaa taccttaaat cccatttctg caaataaagc tatatcttct ttgtatctgt
    39121 gataaaaatc tattgcttca tgacttggat aaaatgttcc tttttctata actctcgtta
    39181 ttcttttact tactttttga gaacctccag tacacatatc tgaacaagaa ggacctttcc
    39241 catctaaatt ccatgctcct tcacattgat ttgctgctgt tgctccaccc cataaaaaat
    39301 cttttctaag tgccataatt ctacctccaa taatttattt atagtaactt tattttaaaa
    39361 taaaaataca tatatataat tcttaattaa tagaactctc ttttacgcta aaacagttat
    39421 caattcctga ctattattta catgcttttg tgtattttct aaaatatcca cataatcaga
    39481 ataattagta acaactactg gtgtttctaa gcaatatcct tcttttgaaa tactttctat
    39541 gtcaaaagtt attaatatct gacctttttt aatcttatcc ccttgattaa caaaagattt
    39601 gaaatattta ccttccaact gaatagtatt taaccctata tgtatcaata gttcacaacc
    39661 attatcagat ataattccta ttgcatgttt tgttggaaat aaagtcatta cagtcccatc
    39721 aaatggtgcc actacttttc cttctttagg tgttattgct acaccttttc ccaatatacc
    39781 tgttgaaaac gctgcatctt gtatctgctc caaagccttt acttcacctt ttattgggct
    39841 atatactact tctcttttgt ttttttctat aacgccttgc ccattatcat ctttagaaat
    39901 ttctactata gcatcatctt tccaaaaaaa taatgttaaa acaaatccta ctacaatagc
    39961 tactattatt gctattatag ttgcataaac acctttcata tctccagttg ttggatttat
    40021 ataattaggt ataccaaata ttccaagtcc acccattaca taacttttta cattcattat
    40081 ccccattaca gctcctgcca caccaccacc tacacagcta tatatgaatg gtgtttttct
    40141 tggtaaagtt attccatata ttgcaggctc tgttacacca catatccctg aaataactgc
    40201 aggtatacac agctctttta tcttcttatc ttttaattta aagtacattg ctgctactac
    40261 tgctgtttgt gcaaaagatg gtacaaatgc tcccactaat attgtgtcaa accccatagt
    40321 cataaggttt gacatagcta ttggtactaa tccccaatgt agaccaaata ttacaagcac
    40381 ttgccacaat aaaccagtta atgcaccaca taaaattggt gaaaagttaa ataaagctaa
    40441 aaatccattt ccaactatat tagaagcata ggtagctata ggccctataa ccataaatcc
    40501 caatggtaaa gaaactaata aaactgccat tggtacaaag aaattcttta ctacatctgg
    40561 tattatttta gcaaacactt tctccaactt agctgctaat aatactatta aaattactgg
    40621 tactactgtt gaagtgtagt tcattgctat tactggtaat cctaagaaag ttatatatac
    40681 tggtgattca aatattgttc cagtaaatag attgtataaa ggttgacctg tagatgctag
    40741 agtatctaat tgaatacctg aatagcataa aactgcacct ataagcattc caataaatgg
    40801 ttttaatttg aatttcttag cagctgtata tccaagaatt attggcatat actggaacat
    40861 tgcatctcca attgcattta acataatata tgcaccatca gtttctccat acaaacccaa
    40921 tgctgtaaat actgcattaa atccttttat cataccagca gcacacagaa ctcctaatat
    40981 tggttgaaaa actcctgata ttatgtctat aaacttatta agtaatcctt ttggcgcatc
    41041 tgagtctgaa ttatctattg tatctcctcc taatccagcc aaatcgcaca caacttcata
    41101 aacatctgga acatgattac caataactac ttggtattga cctgcacttt tcattacagt
    41161 aactatacca tccatttttt ttagcacatc atcatttgct attgactcat cttttaattt
    41221 aaaacgtagc ctcgttatac agtgagataa actgctaata ttatcctttc ctcctacatt
    41281 cgcaatgatt tcttttgata gttgttcata ctttctcatt ttttctcctc cattttcttt
    41341 ttgaaatatg aaggtttggc caacttaatg tcaccaccca atatatataa acctaattat
    41401 taggatttat atctgactta atacttttac ttaccactct ttcaacgtga atagttaagt
    41461 ataatttttc ctcatctgtt aaatcgtagt tgtatttttt atgtataaat tcatccaact
    41521 tttctatgaa tttataagca tttttatatt taacttttat tacatttaat aaatcatctt
    41581 cattattttc atgtaaatta ttactaacaa ttcgttctgc aaaatacttt aagtgagtaa
    41641 taaatctatg ataataaact gaatcttcat caaatactat tctgtaatgg tatttaacca
    41701 tatttgaaat ttcttgtatt atttgtgtaa tctcatatat agtttttata ctttgatcca
    41761 tttgtgcatt aactatatgc agtgctataa atccagcttc atcttctgat agtctaattt
    41821 caaatttttt ttctatataa tccaatgcct ctaaccctat tttaaattca ctcttataaa
    41881 atctttttat atcccaaagt aatacatttt ttacacttac tccttcacga gctctttcta
    41941 ttgcagtata catatggtct gttaaagaaa tataaatact atcatttaat cttttcccta
    42001 gtttttcttt agcatataaa attatttcat ctgataattc catatatctt ataggaatat
    42061 ctgctattag ttctttaaat ttatcagata tatttgggtc tgaaagtttg aatatcttat
    42121 caattttatc ctttgatata tgctctcctg tacgtttttt ataagctaaa cctctcccca
    42181 taactactat ttccttatga ttttcatcag ttgtaataat tacattatta ttgagtattt
    42241 tttcaataat cattgtgatg ccccctttat tagcattaag ttaaaataaa tcttttttaa
    42301 ttatttagaa tatttaaaat gtaatttatt acataaaaaa aaacctattt attacacatt
    42361 aaatagactc ccatgagtcc tgagtaataa ataggtttag cttgtattta aacaatcact
    42421 atccgtttgt attttatttt aattattatt tattatatgt tagcaaattt attttttagt
    42481 gtcaactttt tctcaatatt ttaattttaa ataaattata taaattgaat cttcaaagta
    42541 ctatttttat caataaacaa aataaaaaag gtattacttt ctttaagtaa tacccattta
    42601 tttttaattt aagtggtttt tcttaatctc atcaagtagt ccatcttcta aataaatttc
    42661 taatgcacat cctaccattg cctttactgc tttgtgaagt ttatcataag cttcctcaga
    42721 atttgcataa tttagaaatt ctttttcatg aacatatacc attggactta tatctaaggc
    42781 tatttctgta tacatagtag ggcaaacttg acttacatta cctatatctg tagaacctac
    42841 tggaccatct ttaccttcta atatatctgt tatacctgcc tcaatcaaat tattctttgt
    42901 tatcttttga agtactttgt tatttattat gttgtcaaaa gaattttcaa agtatctata
    42961 agaaagctta gctcctgtca ttaactctgc accttttgca cagtttataa cttttttagt
    43021 tacttcatct aaataacttc tttcagcagc tcttacgaaa aacttacaag ctgctttttc
    43081 tggaactata tttggagcct ctcctccatt tgaaactata ccatgtattc taacatctga
    43141 ttttacatgt tgtcttagtg catttatccc agcaaaagta agttgtactg catctagtgc
    43201 attgactccc atatgagggt gactagctgc atgtgctgat accccttcaa aactaaactc
    43261 taatgaatct atcgccaaag ccttacagtt tatattatta aaagcttcta tatgcatttg
    43321 ataacttaca tcaacatcat caaaagctcc tgccttaact aaatcacatt ttcctcctgt
    43381 tgtttcttca gctggtgttc ctattagtac aatttttcct ttaaaatttt cttttaactt
    43441 acttaatact atacaagctc ctagtgtaga tgctgcaatc cagttatgtc cacaagcatg
    43501 agctggttct ttattttctc catatccagg aagtgcatca tactcagcta aaaatgctat
    43561 ggtaggtcca tcattatctc ctaattcagc tctaaaagca gtctccatat tacaatatgg
    43621 ataaactgta tcaaatccat attccttcat tttttctact aagtattttg atgatatgta
    43681 ttcttcttcc cctaattcag gattgttaaa tataaaatta caaacttctt caatttcttt
    43741 tccaagttca ttctctatct cataagctct cttctttata ctctctatca taatttacct
    43801 cctactagaa tcctagatat aatcctattg tcaaaaatgc cattacaaga attgttaata
    43861 ctaccattaa aggaattacg aatctaaccc actttgacca aggaatattg gctactgcta
    43921 gtgctcccat taaaacacct gatgtaggtg ttatcaagtt agttacacct ataccaaatt
    43981 gataagctgt aactatcatt tgtcttgata catctactaa gtcgccaagt ggtgcaagta
    44041 caggtattgt aagtgatgca agccctgatg ctgatggtat taaaaatgct attaatcctt
    44101 ctattaagaa agttaaattt ataaatatag tttttggtaa tccatttaat aaatttgctg
    44161 cagaatttaa aattgtatct attataaatc cattttctgc tattattacg attccacgtg
    44221 caaatgctat acacaatcct gctgtaatta aatctcctgc tccacttata aatgaattaa
    44281 ctatctcatc ttgtttaagc cctgatgcta ttcctgatat tatacctata gctgtaaata
    44341 tcattgcaat ttcagttatg taccattcta gtcttaaaac tccatatatc ataatcgcca
    44401 tacctattgt gaaaatggct attaccatgg cttctctttt tgtaaattcc tttatatttg
    44461 tatcactatt aaccattaat tcttgattta gtgatatatc atgtactaaa gattttttag
    44521 ggtctttttt aactttattg gcatacatca taacaaatcc tatagatata gccatatata
    44581 ttataaacat aacaactcta aactctattc cagaacctgg tactatttgt gataatgctt
    44641 gtgctattcc tacagagaaa ggattcgtag tagctgctcc aaatccagct gttgctccta
    44701 taaatactgt ggccactgct gtcaatgaat catatcccat actggtcata agtggaataa
    44761 atatcatata aaaaggtagt gtctcttcac tcattccaaa cgtcgtccca cctagtccaa
    44821 atagaatcat tgaaatcggt attattaaaa attgaaactt cttcatttta tttacagtat
    44881 gagctataac agcttctata gctcctgttt tattaactat cccaaaagct cctccaacta
    44941 ttaaaacaaa tccaactact tcagctgcat ctataaaccc gtttataggt gctgtaaata
    45001 catcagtaat tccttgtgga tttgatggtg ctttttcata agttccagct acaacaactg
    45061 accttccgtc tatatctact ctttcaaaat ttccacttgg cactatccaa gtaagaattg
    45121 ccattaacac tattagcaaa aatattattg taagtgtctg tggcatagta aactttttct
    45181 tttttaaact gctcattttt aatctcccca ctttttaaat taaatatttt attattgcat
    45241 ccctttatcc tattttgtat agatgtaaaa aagctcaaca tggatacatg ttgagctaaa
    45301 tgtgctaaat acaacatacc tgcatcttcc ctttatcttg tttaagatag ctctccatca
    45361 aaattcggga taattttgat gacagtactg tacctattaa atacagtccc agcactagct
    45421 tcggacacta gcacttcggc acttactcct tttatattgc ctcattggtt tccaacctct
    45481 tcaatattaa tcttagtaaa tgcaacctct acctcaatct gtttgttaga tgaggtaatt
    45541 cttattaatt tttattaatt caaagtaatt gtagcataat atgtcgagat tgtaaatatt
    45601 ttcagtataa taattcttac tttatgattt cattccattt ttcacaaata attttttctc
    45661 catataattt aaatactgta gtatatttta aatacacaca aactttccac aaattttaaa
    45721 ttattataat acctttataa taaaaaataa aagctatgta aacttatcaa tttacacagc
    45781 tttatataca ttaatctact ccaagtacag ctttaagctt tgatgctatt gtaataactc
    45841 gtggaccaaa tataacttga actgagtttt tctttttaac aactccactt gcacccaatt
    45901 catttaccca gaagccatct tcatagaatc catcaattga atttagctct accctaagtc
    45961 tagttgcaca gtttgtaaca ttttttatat tattacttcc tcctaaacct tctattatag
    46021 aatgtacaag ctttgtatct ttatcatcta cttcctcttc tactgcattg ttgtatgctg
    46081 ttttaccagc atctacagat tgtctttctc tatattcttt cttactataa agcttaactt
    46141 cttctgaact tcttcctggt gtttttatat caaacttctt gattgcccat gtaaatatta
    46201 tataagtaac tataaaaaat attggtatca aaaataataa tggtaacgca tccacttttt
    46261 gtggttgtaa taaattagga aacatatttc taaatgattc tcccattata gaaacattaa
    46321 acatttctgt aaatacatac gtcaatcctg ccagtggtac atatgctaca aaatacaata
    46381 atggtgcaac aaataggaat gtatactcta ttggttccgt tacccctacc aacatggctg
    46441 ttactattgc tggtatcaat atacttattg cttttttctt attagcatta tcagccaact
    46501 tatacatagc taaagctgct gctggtaata ttgcaaaatg tattaataaa cgacctgttg
    46561 tgaagttacg agttatataa gatagtgtat ctgggtctcc catttgagct aatcttatat
    46621 tattaacacc ctcgtaaatt gctccaccta cttccattgt tccacctact cttgtatatt
    46681 ctatagggaa tgcaattaag tgatgtatac caaatggtaa taatgcttta tccattgctc
    46741 caaaagtaaa tgttcctaat aatccactag aacctatcca attaccaagt ttagataatg
    46801 ccaaccctac tggtggccat ataaagtaca ttgctgtacc caatactatt gatgctataa
    46861 acatcataat cattgaaaat cttggacctg agaaaaaaga taatgcacta ggaagttcta
    46921 tgtcaacaaa tttgttatgt actgcactag ctacaaatcc agctattata gctccaaata
    46981 tactacaatc atatgaaaag attcctaaga aagaaccata taaagaactt cgactatatg
    47041 cttctgcctc tcctaatcca gatgccataa acgcttctgc tgtaactgta tctgaattta
    47101 taccatttgc tgctaacaat gtacttataa ctgtgtgcat tcctataaat aaaacaattc
    47161 ctcctaatgc accccatcct ttttcctttt tagataatcc aaaggctata cctgtaacaa
    47221 agaatattgg taagtatctc attaccataa atcctaaatc gtttattact ttaaagaata
    47281 catttataaa tgacccttct tttattagtc cgctaagccc taatgcagaa acgttttcca
    47341 tatttgcaaa agctgaccct aatcctataa atactcctgc tacaacaagt aatataacag
    47401 gtatcatcat tgctccagaa agtacttgta atttatcctt catagtatta tttcctccat
    47461 ttttattgaa atgccaggct atataatgca tatatatata cttctattgc tttgtataat
    47521 gtatctatac ttatattttc atttgcctga tgtgaatttg gattatcccc ctctattgtg
    47581 ggtccaaatg caactccatt ttttaacact cttgcataag atgcagctcc ttttgtaaaa
    47641 agttcggctt cttttccaaa accttgttta tatgcatttg ataatttatt tattaattca
    47701 ctatctggtt ttacatacag taattttaaa tccttatata tatcaactat taaattttct
    47761 ttttttccaa tttcattaat tctattagtt ataaactccc aacttatacc cttaggatat
    47821 ctaaagtcta ttttcatatt caattcattt ttctctaaga agatactcat tatacatatt
    47881 gtagaaactc ccatatctac atcttcttta tacatcccta atttttttcc atgattatca
    47941 tctgtaaagt atgaatttaa aatatccctt attataacgc ctttatcatt tagtttttta
    48001 attttctcta aatcttttgc taaactaaat gcacagttat atgacctgtg tggatttctc
    48061 gataaagccc tttctcctgt atatcttact aatactttac cttcatctaa ttcttctatc
    48121 tctgtataat aagctaatga ttctactaaa tctttcttat cattagtttt aaaaacagcc
    48181 tcaattttgt cacatacaaa tccatcttct ttgtttgact ttatatcaac taaattgtga
    48241 tttctaaatt tatctttatc tattttcttt cttagattga agtataaaat acctttttct
    48301 ccatttacta ttgggaagtt tccatctgga gaaaatgcaa attttggctg ttcattgaca
    48361 ctaaagtaat gctccataca ttcccaagtc gtctcttcag ctccacccac tatcaatcta
    48421 atctttctct tcggcttttc attaagttct cttaagaaaa gtaatgcata taaaatccct
    48481 atcaaaggtc ctttgttgtc attaactcct ctaccataaa gatagttatc tttttgacat
    48541 actttaaaag gtttactctt ccaaagttct ttattatata ttggcaacac atctatatga
    48601 ttaagcaccc ctactacttc ttctccttct ccatattcta tatgaattgc atatccatca
    48661 aagtctttta caacaaaatc tttgtctttt gctatttgaa taagcttatc gaaggcattt
    48721 cttatttcta tcccaaatgg ttgatttata tctgttgtac tttcatctct aattgaaggt
    48781 atttcaacta aacttctaat atcatttatg atattttctt gattggattt taaataatca
    48841 ataattagtt ctttgtttac tatcatctta attatttatc acccctttat attaatttta
    48901 caaagcattt cagttcatat ctacattata aattttgaat attatttttt caatatttca
    48961 aatacttctc taagttacca taatgatacg gtaaacaatc aacagattat gacaaaatca
    49021 tactatacct attactaata ttttaactta tctattacgt cttttttaat gtaagtttat
    49081 tccaaaaata cataaaaaaa gatgtagcct tagctacatc ttcttatatc tattcaactt
    49141 ccattattat gctattaata tctacatttc caggttctat cctatctttt actgttctct
    49201 taacagaatc agcatcatcc attataacta caggagttat tatatttaaa tcatttgacg
    49261 ttataaaatc taaatctact tccattatta aatctttagc ttttacctta tctcctgcct
    49321 ttaccttaac atcaaacggc tttccttcta gtgacattgt ctctaaacct acatgtatta
    49381 atatatttgt attatcatta ctcttaatca ttattgcatg ctttgtatca aatacattga
    49441 ctatttcacc atcaacagga gaatataact taccttcact tggtattatt gctactccat
    49501 ctcccatcat cttttgggca aaaactgcat ctggaacatc ttctaagttc actaaatttc
    49561 ccttaaaagg gctatatatt ttctcaatct tattttcttt tttcttaaat atattaaaca
    49621 taacacactc tccacattat aaaattttat gataatattt ttcttaaagc ttttgctaca
    49681 ccatcattta cattagtatc agttacaaaa tctgccatag attttacttc atctgtagca
    49741 tttcccatag caataccaag acctgcaaat ttaatcatgg atatatcatt ttcattatca
    49801 cctatacaaa taacttcttt gctatctata cctaatatat cacaaaattt tttaactgca
    49861 ataccttttg aagtaccttt actcataact tcaaagttta tctttccaga acttactact
    49921 tctaaatctt cttgtttttt aaattcttcc tttgcttctc tcaaagaatc tacgttttct
    49981 gagaaaagta tacattttaa aatttcatta tcagcttttt ctaataaagt ttcacaactt
    50041 gcattttcta ttatttcaat tctgtcttct tctttatttt tactatttat taatctatat
    50101 ggatgttctt cacctatttc attgttggat ataactattt tacagccttt aaaatgtttg
    50161 tttaaattat acttttcacc tattgtatag attttcttta gagcttcttt gctcaaaacc
    50221 ttcttatact catatcccag taatttaaaa tatgtcccat tagttgatat aatatatata
    50281 tcatccccta aaacacttgt aaaatatttc atagcattat aaggtcttcc cgtaactaag
    50341 gctaccttga tacccttttc catggcttct tttagtgctt taatattttc ttctgatact
    50401 tcaaatcctt tacccatcaa agtaccatcc atatcagtac aaattaattt atatcgcatt
    50461 tgtatcacat cctagtaaga tttttctaat atttcaacaa ggtcttcttt ctttccaaaa
    50521 ggtaaaaatc ctaaaactgg acagcttgtt atttgttcta attgttcctt tgttacatta
    50581 aactcagaca tttttgtctt aagccccact ctctttaaaa aactttctat ttcatttcct
    50641 aaagttttag ctgcttcatc atcttccact ttttctaatt cttggtttag tattctcgct
    50701 actttggcaa atttctctat attatcctta tagaactttt ctgcaaattt tgggaacact
    50761 atagccaaag cctcaccatg aggaatatgt gtaactcctc ctattatttc acttagtgga
    50821 tgtggtgctg ctgctccagc atttgccagt gaacttcctc ctaatgtatc tgctactgct
    50881 aactttgttc tgtattctat attattttta tcttccatta ctttaggtag atagttaatg
    50941 actaattcca tagatttttc agacattatc tctgtaaaat cattagcatt tggatttata
    51001 taactttcaa atgcatgagt aaaagcatca aatgctgttg atgcagtaat tctctctggc
    51061 agagttaaca taagctctgg gtcaactata cattcttttg agaaacaatt ttgatgaaat
    51121 attgtatttt tctcgcttcc tctcgatatt actgctgctt gtgtaacttg gcttcctgtt
    51181 ccagaagtag ttgatacagc tattaatggt aattcttttt cacttacctt atcgtatatt
    51241 ttaaatggat ttgtatatgt actaaacata taatcccaat ctattttttc taaaccaaaa
    51301 gtcaatgcta atacttttgc agtatctata cttgaacctc ctccaacagc taaaacaacg
    51361 tctacagatt cttttttagc taattcaaac ccttcgttta ttaattctac agttgggttt
    51421 ggctctactc tctgaacctc ccatgactaa agtcacaggg ttcctgcttc acagaatttt
    51481 gcatactaaa atatgtctta ctaattctcc acaggccatc cccgtagttc ctacggttct
    51541 tacataagtt atttattttg aattgttatt agtcttaatc cttcttttaa tatatttata
    51601 cttgcattta tatctctatc gtgagtttca ttacaactag gacaaatcca ttctcttata
    51661 tttaagtcct tcacttcctc gtttttatac ccacatttat tgcatatttg tgaacttgca
    51721 aagaatttac ctacctttac aatttgtcta ccatgccaat tagccttgta ttctaattga
    51781 cgaataaatt ctgaccatga tacatctgaa attgagcgag atagtttacg atttttaatc
    51841 atattcttta cttgtaaatc ttctatgcaa ataatatcat tctcttttat taatttagta
    51901 gataattttt gcagaaaatc atttctttga ttagctatat gttcttgaag tcttgcaact
    51961 tttaatcttg
//
LOCUS       DAELTA010000008        48434 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.008, whole genome shotgun sequence.
ACCESSION   DAELTA010000008 DAELTA010000000
VERSION     DAELTA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.008"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(167..928)
                     /locus_tag="KPG13_000396"
     CDS             complement(167..928)
                     /locus_tag="KPG13_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0984143.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1147..1623
                     /locus_tag="KPG13_000397"
     CDS             1147..1623
                     /locus_tag="KPG13_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0984144.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1721..1873
                     /locus_tag="KPG13_000398"
     CDS             1721..1873
                     /locus_tag="KPG13_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG0984145.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(2033..2221)
                     /locus_tag="KPG13_000399"
     CDS             complement(2033..2221)
                     /locus_tag="KPG13_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG0984146.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2582..2932
                     /locus_tag="KPG13_000400"
     CDS             2582..2932
                     /locus_tag="KPG13_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0984147.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2951..4594
                     /locus_tag="KPG13_000401"
     CDS             2951..4594
                     /locus_tag="KPG13_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG0984148.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4675..6732
                     /gene="recG"
                     /locus_tag="KPG13_000402"
     CDS             4675..6732
                     /gene="recG"
                     /locus_tag="KPG13_000402"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG0984149.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6791..7345
                     /gene="rsmD"
                     /locus_tag="KPG13_000403"
     CDS             6791..7345
                     /gene="rsmD"
                     /locus_tag="KPG13_000403"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG0984150.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7347..7844
                     /gene="coaD"
                     /locus_tag="KPG13_000404"
     CDS             7347..7844
                     /gene="coaD"
                     /locus_tag="KPG13_000404"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG0984151.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7848..8372
                     /locus_tag="KPG13_000405"
     CDS             7848..8372
                     /locus_tag="KPG13_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984152.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8716..10635
                     /locus_tag="KPG13_000406"
     CDS             8716..10635
                     /locus_tag="KPG13_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0984153.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            10648..10935
                     /locus_tag="KPG13_000407"
     CDS             10648..10935
                     /locus_tag="KPG13_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984154.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            11068..12327
                     /locus_tag="KPG13_000408"
     CDS             11068..12327
                     /locus_tag="KPG13_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG0984155.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            12330..13070
                     /locus_tag="KPG13_000409"
     CDS             12330..13070
                     /locus_tag="KPG13_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0984156.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            13393..14703
                     /locus_tag="KPG13_000410"
     CDS             13393..14703
                     /locus_tag="KPG13_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0984157.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            14801..15718
                     /locus_tag="KPG13_000411"
     CDS             14801..15718
                     /locus_tag="KPG13_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0984158.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            15734..16543
                     /locus_tag="KPG13_000412"
     CDS             15734..16543
                     /locus_tag="KPG13_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0984159.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            16601..18349
                     /locus_tag="KPG13_000413"
     CDS             16601..18349
                     /locus_tag="KPG13_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG0984160.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            18611..19633
                     /locus_tag="KPG13_000414"
     CDS             18611..19633
                     /locus_tag="KPG13_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG0984161.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            19638..21944
                     /locus_tag="KPG13_000415"
     CDS             19638..21944
                     /locus_tag="KPG13_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0984162.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            21916..23031
                     /locus_tag="KPG13_000416"
     CDS             21916..23031
                     /locus_tag="KPG13_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984163.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            23049..24467
                     /locus_tag="KPG13_000417"
     CDS             23049..24467
                     /locus_tag="KPG13_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG0984164.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            24482..25690
                     /locus_tag="KPG13_000418"
     CDS             24482..25690
                     /locus_tag="KPG13_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984165.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(25764..27152)
                     /locus_tag="KPG13_000419"
     CDS             complement(25764..27152)
                     /locus_tag="KPG13_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG0984166.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            27493..28827
                     /locus_tag="KPG13_000420"
     CDS             27493..28827
                     /locus_tag="KPG13_000420"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG0984167.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(28969..29886)
                     /locus_tag="KPG13_000421"
     CDS             complement(28969..29886)
                     /locus_tag="KPG13_000421"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG0984168.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            30094..30978
                     /locus_tag="KPG13_000422"
     CDS             30094..30978
                     /locus_tag="KPG13_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG0984169.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            31191..33032
                     /locus_tag="KPG13_000423"
     CDS             31191..33032
                     /locus_tag="KPG13_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG0984170.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            33337..34044
                     /locus_tag="KPG13_000424"
     CDS             33337..34044
                     /locus_tag="KPG13_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984171.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            34038..35348
                     /locus_tag="KPG13_000425"
     CDS             34038..35348
                     /locus_tag="KPG13_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0984172.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            35465..36148
                     /locus_tag="KPG13_000426"
     CDS             35465..36148
                     /locus_tag="KPG13_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984173.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            36141..38528
                     /locus_tag="KPG13_000427"
     CDS             36141..38528
                     /locus_tag="KPG13_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0984174.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            38808..39950
                     /locus_tag="KPG13_000428"
     CDS             38808..39950
                     /locus_tag="KPG13_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0984175.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            39969..40493
                     /locus_tag="KPG13_000429"
     CDS             39969..40493
                     /locus_tag="KPG13_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0984176.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            40593..41411
                     /locus_tag="KPG13_000430"
     CDS             40593..41411
                     /locus_tag="KPG13_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0984177.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            41489..41800
                     /locus_tag="KPG13_000431"
     CDS             41489..41800
                     /locus_tag="KPG13_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG0984178.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(41977..42660)
                     /locus_tag="KPG13_000432"
     CDS             complement(41977..42660)
                     /locus_tag="KPG13_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984179.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(42753..43394)
                     /locus_tag="KPG13_000433"
     CDS             complement(42753..43394)
                     /locus_tag="KPG13_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0984180.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(43568..45571)
                     /locus_tag="KPG13_000434"
     CDS             complement(43568..45571)
                     /locus_tag="KPG13_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG0984181.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(45724..46362)
                     /gene="recX"
                     /locus_tag="KPG13_000435"
     CDS             complement(45724..46362)
                     /gene="recX"
                     /locus_tag="KPG13_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG0984182.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            46638..47327
                     /locus_tag="KPG13_000436"
     CDS             46638..47327
                     /locus_tag="KPG13_000436"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG0984183.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            47328..47900
                     /gene="yqeK"
                     /locus_tag="KPG13_000437"
     CDS             47328..47900
                     /gene="yqeK"
                     /locus_tag="KPG13_000437"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG0984184.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            48017..48367
                     /gene="rsfS"
                     /locus_tag="KPG13_000438"
     CDS             48017..48367
                     /gene="rsfS"
                     /locus_tag="KPG13_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG0984185.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
BASE COUNT        18867 a         5342 c         8225 g        16000 t
ORIGIN      
        1 ttttgtattg attataatta ttatacaata aagattctgt ctcaaaatag agagagaaac
       61 ctgtttttca ttgataaaat gataaaaata tatctgattt catacaaaaa agagtgtctc
      121 atgaactaaa aagttcattt tgagacacca cctttattat tttatattat ttatctcttg
      181 tatatattac tatagcatcc cttaaaaact tagctaatcc aggttgttct ctatcataat
      241 atgatgtaaa acgttcatca tatacataca tctgtgctag agaggaatga gcttctttag
      301 agtatgtgtt ccaagtgtaa cacaaccatt tatgatgtaa ttttgcaact tcctgagcca
      361 gttcactaga ggcatcacca gttttaaaag cttttttaag tttactaatt atttcgatat
      421 ttaggttttg ccaattttca taatcttgct tagacatatt tttaagtttt ttatttgatg
      481 tatctataat atctttacca tatttctctc taatttcagt tccataattt ttttcatttt
      541 catcaatcat cttttcttta aaaccttcaa atttttctat atctgtcata gtgtattttc
      601 cctccttcag agctatagtt ttagttacat tttctattag taaatcaatt tgctttcttt
      661 tggctagtaa tttattatgg tgttctttta acgcacttaa actatcgaag tttggtgaat
      721 ttataatgtt ttttatattt tctaagttaa ttcctaattc tctataaaaa agtatttgtt
      781 gaagtttatt aacttcattt tgaccatata tacgataacc tgaagaattt attttaagtg
      841 gcttaagaag acctatttca tcataatatc taagtgttct agtacttata ccagctagtt
      901 tacttaattt ttgaactgta tattccataa gtagtacctc ctataaatta aatatacact
      961 tttacgcaac gtaaatgtca atacattaaa atataaaaat ttatatcttt taataaaaat
     1021 ataaaaaaat atctttactg gatattttac acacaactgt caatactcac tagtgaaaat
     1081 taaaaaataa aatttataga ctatctttac taaaaaatag aaggtctata gtctaggagg
     1141 aactgtatga atttaaaaag aagcaatgaa aaacgagaag agtacagaag aatgtattca
     1201 gataaagaaa gttttttaag tttgattcaa aattttacaa gtgtctcaat agcaaaagaa
     1261 ttaactctta aaaatataga gttagaaaca tcttttatat gtgaatataa aggaaaagaa
     1321 gttgatataa tttataaggt attttctaaa agcggcaaag tatcacatta tatagtgtta
     1381 gaatttcaaa cagaaatgga tactgaaatt gtcccaagat tgaaatcgta tagagaacaa
     1441 atttggaaaa gttttattat gaaaaaaagc cttgaagaaa tagaagataa aaattttaaa
     1501 cttcctaaag ttataccagt tgttttatat agtggacctg aaaggttaag tagaaaaaaa
     1561 ggaattttgg atattgtaga atctatatcg gagttcagta ataattctag tatgaaaaat
     1621 taaaattaca taatgtgttt attggtctaa aatggaaaac tgtcgcataa acttgaatat
     1681 gaaaatattt atagatacag gttctatgtg gaggtgttcc atgagaaata acaagtctaa
     1741 ggtattgcta ggaatacttt gtctagcaac aggaatggct gtaattctgt caatgttgct
     1801 tcctggctgg atttggtcag cacttaccgc attaatactt attggatgtg gagcattatt
     1861 atttttttgt taaaatagtt ttaactataa agtgtggggt gagtattgat gaaagtagta
     1921 acaataaaat taccaaaatg gatttcaagt ataatcctaa aatttatgag aaagagtaaa
     1981 aatgattaaa ttaaaaagcc tacttacgga agtaggcttt taaacgtggt tactaagctc
     2041 tttcaatttt accagaacgt aaacatctag tacaaacttt tactctctta ggagctccat
     2101 ctataatcgc tcttacgctt cttaagttag ctgaccatgt tcttttatta tgtttatttg
     2161 agtgtgatac ttggtttcca gaaaccttac ctttaccaca tacgctacat acttttgcca
     2221 tctacgcaca cctccttaaa atgataacta tttaaagact aaaaacatat ttatattatc
     2281 ataaataaca aaaatttgca atagaaatat atatttgcct tacacatatt gaagaatttc
     2341 ttattatcat ataaaattat atatataatt ttatataaag taaatatatt tggaaaataa
     2401 ttattatttt tctttgaata tatatactat agaagaaatt ttatccaaaa acttttctag
     2461 gtttaaatta taccatattt atagtatgat atgattataa ttttatataa taaataagta
     2521 aaaatagtaa aaaataaatt aagaaatgtt tttaataaaa ataagcttaa gggggtcaac
     2581 catgagtgca aaggtaacga atcaatatgg aaatatagaa atagataatc aagtaatagc
     2641 tcaagtcgta tatagagctg caatggaaag ttatggctta gtaggtttag catataagtc
     2701 taaaggaata gtagagctat taaaaggcga gaacgcaact aaaggtgtaa gagttgagga
     2761 agtagaagaa gatgccatag caatcgaact ttatgttata atacaatatg gtacaaatat
     2821 atctactgtt gccaataata taatagatag agtaaaatat gtagttgaaa aaatgactgg
     2881 cgtaagagtt actagaattg atattaatgt acaaggaata agagttaagt aatttcatag
     2941 gaggaaacaa atgattcaat atatagatgg taaaaggtta agggagatgt ttatttcagg
     3001 tgcaaacaat ctccaaaaca ataaagagtt agttgacaag ttaaatgtat tccctgtacc
     3061 agatggagat acaggaacta atatgtcatt aacaatatct tatgccttaa aagaattagc
     3121 aaaagttgaa aatgataata tatcagatat cggaaaagca ttatctaaag gttctttaat
     3181 gggagctaga ggaaactcag gagttatact ttcacaaata atcagaggta tagctaaatc
     3241 tatagaagga aaaagtaaat tatcaacaga agatttagca aaagctttta aaaatggttc
     3301 agacacagca tataaagctg taataaaacc aattgaagga actatactta ctgtagttag
     3361 agaaagtgga gagtttgcaa taaaaactgc gaaaaaagag aaagatgtag taaagttttt
     3421 aagtatgctt gtgaaggaat caaattcttc attagaaaga actccagacc ttctaaagaa
     3481 tctaaaagaa gctggtgtgg ttgactctgg tggaaagggt cttgtactta tatatgaagg
     3541 aatgttagct tctataaaag gcgataacat agaaataaaa aatgttgacc tagatactaa
     3601 tatttcaaca agtatggatt ttgcaaagag tactactagc acagatgata taaaatattg
     3661 ctattgtaca gaatttattt tagaaagttc taaggtagaa gatactaaaa taagagacat
     3721 aatgatggct tatggtgata gcctagcagt agttggagat gatggagtca taaaggtaca
     3781 tgtacacact aatgacccag gaaatgtact tcaagaagct ctaaagtacg gtcaattatt
     3841 aactataaaa atagaaaata tgaagttaca acatgaaaat acattactgg atgtagaaga
     3901 aaagaaagaa aatgatagtg aacctttaga agaagaaaaa gagtttgggt ttatagctac
     3961 ttctatgggt gaaggtttgg caaatatatt taaagatttc ggagttgacc atataataga
     4021 aggtggtcaa actatgaatc caagtactga agattttatg aatgctataa agcatataaa
     4081 tgctaaaaat atatttattt tcccaaataa cagcaatata ataatggctg ctaatcaggc
     4141 aaaagaattg agtgataaaa atataatagt tatacctaca aaaaatacgc ctcaaggatt
     4201 tgctgcatta gtaactttta atggagaatt aagcgaagat gaaaataaag aagctatgat
     4261 gaatgcttta aactcagtta aatcaggtca agtaactttt gctgttaggg atacagtgat
     4321 gaatgaaatt gatgttaaag aaggtaatat aattggaatt gcagaaggaa atttattatc
     4381 agcaggagat tatgtggatg aagtaacatc aaaccttatt gaaaagttgg ttgatgaaga
     4441 tactgctata ataacattat tctttggtga agatgttaca gaatctcaag caaatgaact
     4501 acgtacttct ttagaagaaa agtttgaaga tgtagatgta gagttatatt atggaggaca
     4561 acctctttat tattacttaa tttcagttga ataatgatta taagaacctg tatgaccaag
     4621 tagcttttat aaaattggtt gtacaggatt tttgtgtagt aggtgatata tttgttggat
     4681 ttatataaag atgttcaata tgttaaggga ataggaccta aaaaagcaga taaattaaat
     4741 aaactgggta tatttacact aaaagaccta ttgtattatt ttccaagaca atttgaagat
     4801 agaaataatc taaagaaaat agcacaatta gaagatggtg agaaagttac gataaaagca
     4861 gtaatatcaa gtataaatac atttagccca aaagaaggta tgactttaac aaagattgat
     4921 gtaaaagatg agacaggctc ggctaaatta gtgtttttta ataagagtta tataaaaaat
     4981 acttttcgtc caggagattc aatattagtt tttgggaagg ttaagaaaaa atttaataac
     5041 ttagaactta catcatgtga attagaatat cttactaatt cacctaagaa tacatgtaga
     5101 tttatgcctg tgtaccagct tacatacggt gttacaaata aagaaattat gagtataatt
     5161 agaacagtac ttgaagataa agaattaatt atacaagaat atatgccaca aagaattata
     5221 gaaaaatata ggctatgtag tatagatttt gcagttagaa acatacattc accaagtagt
     5281 aaggaatcat taaaaatagc tttatataga attgtatttg aagaattact tatactacaa
     5341 ctagggttgt ttgtatttaa aagtggcaga aataaagaag atggaataaa gtttgaaaca
     5401 agtaaagact taaaaaaaat aataagtgct ttaccattta aactgactaa agctcaaaac
     5461 agggcattag atgaaattat acaagatatg aatttagaga aaataatgaa tagattggta
     5521 caaggtgatg tagggagtgg taaaacagta gtagctctct tagcacttgc aaattgtgtt
     5581 ttaaatggtt atcagggcgc tttgatggca cctacagaga tattagcagg gcaacattat
     5641 atttcactta ctgagtctct aaaagatttt ggaataaatg taggtctatt aataggtagt
     5701 cttacaaaaa aacaaaagga tacagtattg gaacaaataa agaataatga gattgatata
     5761 ttaataggta cacatgcact aattgaagat aaggttgaat ttaataatat tggtttagtt
     5821 ataacagatg aacaacatag atttggtgtt atgcaaagga gcaaactttc attgaaagga
     5881 gcaaatcctg atatattagt aatgacagca acaccaatac caagaacttt agcacttata
     5941 ctctatggag atttagatat ttctattata gatgaacttc ctccaggaag acagccaata
     6001 gaaacaattg ctattgaaaa aagtaagcga gatagagcat ataataatct tgtaagaaga
     6061 gaggttgaat caggaagaca agtgtacatt gtatgtccct tggtagaaga aagtgaagcc
     6121 atagaagcca aatcagcagt tgaacttgtt gaggagttaa gagcggaata ttttcacgac
     6181 ttaaggcttg ggttgcttca tggtaaaatg aagtcaagtg aaaaagatga agtaatgaga
     6241 ctttttaaag ataaagaaat agatatttta gtttcaacaa cagtaataga agttggtgtc
     6301 aatgtgccaa atgcaacatt aatgataata gaaaatgcag aaagatttgg acttgcacaa
     6361 ttacatcagc ttagagggcg agttggaaga ggtagtcata aatcgtattg tgtactaatt
     6421 tatgattcta aaacagatgt atgtagacaa agaatggcta taatggaaga gaccaatgat
     6481 ggttttaaga tttcagagaa ggatttagaa ataagaggac ctggggaatt ttttgggaca
     6541 aggcagcatg gtcttccaga acttaaggta gcaaacttat ttaagcatat aaaaatactg
     6601 aagttagcac agcaagaagc tcgatatata ttgggtgaag ataataatct acaattaaaa
     6661 gaaaacatgg cactcaaaaa agaaattata gataaattta aagatacatt gaaagaaatt
     6721 tcattaaatt aattttaaat tgtgttaaaa tattactaat gtaataatgt gctgaaggga
     6781 gattgtcata ttgagagtaa tttcaggaaa agcgagagga ttaaaactaa atactccaaa
     6841 aaatgaagat gtaaggccaa ctacagatag agtaaaagag tctctattta atatgataaa
     6901 ttcatatata atggaaagtg aagttctgga tttatttgct gggactggtt ctttaggaat
     6961 agagtgctta tctaggggtg ccaagtcatg tacttttgta gatattagta aggaaagcat
     7021 agatattgtt aagtccaata taaaaaaggc aagagttgaa agtgaaagta ttattttaaa
     7081 ccttgatttt aagactgcaa tagataaact taaattacaa aatagtaaat ttgacataat
     7141 ttttatggac cctccgtatt ataaaaatat gtttatagaa gctattgaaa aaattgataa
     7201 ttctaatcta ttgaacgaag atggaataat agttgtagaa catgatacga atgatttatt
     7261 tccagataag atatgtaaat tggagaaaac tagagataag aaatatggaa atactacatt
     7321 aactttttat aagatggagg cataatatgg aaaataaacc tagaaaagct atatttgcag
     7381 gaagttttga cccaattact aatggacatt tagatattat ttgcagggct tcgaagttat
     7441 ttgacgaatt acaaatagga gtattaaata atcctaataa aaagggtcta tttagctttg
     7501 atgaaagagt aaaacttata gaaaaaagta caagccattt aaataatata aaagttgtga
     7561 cttttgatgg tttattaata aattactgtc aggaaaatgg gattggagca ttagttagag
     7621 gtgtgagaag tggtgctgat gttgattatg aacttcagat ggcacatatg aatagagagc
     7681 ttaatccaga tatagagact ataattttac caagttgtac aaaatattca tttataagtt
     7741 catctttaat aaaagaggtt ttgctctttg atgctgatat aaaaaatttg gttccaaaga
     7801 ttgttttgga agaattaaag aaaaaaacta gtgggggtaa ttgatatatg aagatagatt
     7861 tagaaatgat tgaactattt gagcagttag aggagatatt tagaagtgct tctacaatcc
     7921 ctttctcaca taaatgcaca gtggataaag atgaggtttt agcaataatt aatgatataa
     7981 gaactttaat accagaagaa gtaacacagg cagtatggat taataaagaa agaaataaaa
     8041 taataagtca agctaagcaa gatgctacaa atatagttga acaagcgaaa aaagaagctg
     8101 agagaataca acaagaatat caagataata tagaggaatt aaagaaaaat tctgaagatg
     8161 tagtaaaggc atatgtagaa tccagtgaac ctgttgttca agcagaccaa agagcaaaag
     8221 atatagttga tagagctgag agaatagcca atgagataag aattgggtca attgaatatg
     8281 cagaggatgt gctttctagt gttgagtata atttaagaga aatattagaa gaagtaaaaa
     8341 gagataaagc tgaacttaga cctaaaaaat aaattttaaa taaataaaaa tggagctatc
     8401 acacaaaaaa ttccaattta tgtgtgatag ctctctttta atatagcaat tttttgttat
     8461 ataattgtgc tttttagatt acttatatct tattttgata ttataatgta gtttaatctt
     8521 ttatttgttg aaatcaactt atttatttgt ataaataata taggttattt tcaaattttt
     8581 ttaataattt taccactgtc ttcttttaga ttagagttag actgtgcaaa attataaaat
     8641 aatactaaat catgtgcaat tatttggtat ggcactacat gtagaaaata tagataataa
     8701 aattaaggag gtagtatgaa ggaaagggct aaaaaaatat tagattttat tattaaaaat
     8761 aacaatgtaa catcacaaga actacaagaa aagtttaatg tttcaaagag aactatttat
     8821 tacgatattc tagctataaa tgaacaacta ggaaaaagtg gtaatataaa aaatgtaaaa
     8881 cacaaattta tatttgaagg taatttatgt gatgcaagga aaataatttc aactgaagaa
     8941 gataaatttt tagatagtga ttacagaaaa acttttatat taaacaagat attacttggc
     9001 gaaaaaatat caatagaaaa actaacaaat gaaatgttac tttcaaaaaa cacagtagta
     9061 caaacaataa ctgatgtaaa aaaatacctt caaacaatgg ggttaagatt agagtataag
     9121 ggaaagtaca aaattatagg tgatgaatac gttattagag agctgtttct gataattgtt
     9181 caagaaaata tattggaaat taattctata agtgaggaag taagtagttt cgatacaaaa
     9241 ggacatataa aacttactga ttattcttta ttaaatctaa ctaagtttgt ggaatttcta
     9301 aataaaagga taagagatgg gaaaacatta tactcatata aatatctaaa tgaagctaaa
     9361 aaaattagtt actttagtaa ttgtaaagaa cttttatgtg aagaagctaa tgaaaatgaa
     9421 caagcctata tatgcactta cataagttca cttcccagct taaatagtga agttaaggaa
     9481 gatgttgttg aagaatatgt agataaactt atagataagt ttgaagtaaa tacagcaata
     9541 aaattagaaa gtaagcatga gtttaagaaa aatattttaa ggcacctcca ttcttcttac
     9601 aacagaatac gatttaagtt tcctattaga aatccaatgt tagatgagac taagtataaa
     9661 catgagtcac tttataaaat tataaaatca attatagaaa atgaagaaga gtttcctgta
     9721 tttgagggga taagagaaga agaaatagga tttatagcag cgtattttgg aggatattta
     9781 agagggtcta gggataatgg tctaagaaga aataaagtct tactagtttg tcctaatggt
     9841 cttatggtat caaagagttt ggaaatacaa ctatataaat acataccaac catagaaata
     9901 gtaggtatag tatccattaa gcaactaaaa gaagtaaatg tatactacga ttacataatt
     9961 acaactatag atatacaaaa tgtaaacaac gtaattgttg taaatcctct tttaacatca
    10021 tcagatgttc aattacttat gaacaaacta ataagtgtaa aagaaaacga gaagtatttc
    10081 aatcttgaat taataataca agctataaga aaaaatggag ttatcaataa tgaagaagca
    10141 ctaaaagctg atttattaaa cataatacac aagatagatg aaggagagat gtatcaacca
    10201 atgttaaagg aattaattaa tgcagaaagg gtcaatataa taaaaagtgt tagagattgg
    10261 aaagaagcaa taaaaatagc ctcaaagcca ttgttagaag ataactctat agaagagtta
    10321 tatatagaaa atatgataaa gtcagttgag aaatatggac catatattgt tttagctgat
    10381 agatttgctc taccacatgc aagttctaaa gaaggagtaa acaagcttgc tatgtcttta
    10441 ctaatagtag aagatgaagt tgatttatta ggaaaaccag taaatatatt tatggtatta
    10501 gctgctgtag ataacactac tcacattaga gcattagcat cactatcaga gatgatgtac
    10561 gaggaagaaa atgtaaaact aataattaat ggtgataaaa gttcaatact agaacttata
    10621 aataagcaaa attaggagga atgaaaaatg aagatattag cagtatgtgg ttttggtgta
    10681 ggttcatcaa tggtattaaa aatgacaata caaaaggctc taaagcagtt aggtatagaa
    10741 tgtgaagtag aaaatacaga tataaattca gctagaggtg ctgattgtga ctgtatattc
    10801 acaagtcaag aattgttaaa tgaattaaaa ggaacttgta atgtagatgt atatccagtt
    10861 aaaaagtaca tggatttagc agaagtcaca gaagttgttc aaactttttt aagtgataaa
    10921 aatgataaga aatagtataa gttttaaaaa gtaaatttaa gttaatcaga ataaaataaa
    10981 aacttactaa attcaaaaat agtaagaaag agaggaggga tttagtggta gaaaacttaa
    11041 acagttgacc tatcactaaa aaatataatg cttgatttta tttcaaataa tattttacgt
    11101 aatccatcta tgttaattgg attaatagca gcatttggtc ttgtactaca gaagaagaaa
    11161 gcatctgatg ttataaaggg gtcactactt gcagctttcg gaattataat tatggagact
    11221 ggaacaggta tgttagttag ttcaatagca ccaattaatg gagctttcca aagtataagt
    11281 ggtggtgcag taacaaaagg tttaagtgat gttactttta ctgcaagtta tggtggaatt
    11341 ataggtttag caatgtttgc gggtttagct attcacctaa tgatagcaag atttactcct
    11401 attaagacaa tctttttaac aggtcacatg ctttattggt tcccattcat atttgtagct
    11461 tcaggagttg aagctggatt aaatggtgga gtattaatag cttttgcagc agtattatca
    11521 gcactctatt ggtcaataat gccttggtta ttaagaaaat atgtgtttga tgttatagga
    11581 gatgattctt ttacattagg tcatccatct ggttgtttag cacttatatc aggatttgtt
    11641 gctaaattag taggaaataa agaaaaatct acagaagata taaacatacc tcaaagttta
    11701 tcatttttta gagaaatatc tataagtggt ggaatggcag tgtttttaat gtttatagtt
    11761 gttgggatag taataccagg tgcgtttaaa gatagtaatg agtttattgg aacgtatgca
    11821 ataaagcaag gtctttcttt tggagcaggt cttataataa tgcttcatgg ggtaagaatg
    11881 ctaataaatc aaataatgcc agcatttcaa ggtatatctg aaaaagttgt gccaaatgca
    11941 ctacctgctt ttgactgtcc aatattattt aattataaac caaatgctgt attaatggga
    12001 ttcttagttg ctatgctaac atctacagct atgattttaa tatgtaacta ctttaatgta
    12061 tttaatttgc tacttatacc acttgttata acttcattct ttgagtgtgg agcagcagca
    12121 gtaataggag aatcacaagg tggtatacgt ggagctataa taggtacagc tatagctgca
    12181 atatttatgg tagttttagt aggaatatct gctgtaattt actcaggaac tattcaaaat
    12241 tggatactaa tatttggagg taatgacctt tcattatttg gtataatcag taggtttata
    12301 ggttcactat ttggaggaat attatagata tgaaatttaa atacatagat gagataagaa
    12361 gattaattga tataatagaa aacgaagaaa aagtcaatat ggaaaaagca gtacaaatct
    12421 ttgttgaagc aatagaaagt aaaaatgcta tattttcatt tggagcttca catgcaggaa
    12481 tattaacaga agaattattc tatagagcag gtggctgtgt tcttataaat ccagtatttg
    12541 caaaatctct aatgttagat acagcgccta taactcacac aagtaagatg gaaagattag
    12601 aaggatatgg tacagctcta gctgataaaa ctccaattaa aaaaggagat gtagttatag
    12661 ttcattcagt atctggtaga aatccagtaa gtatagagtt tgctatgaaa gctaaggaaa
    12721 ataaggcaat agtaatatgt ataactaatt taagttactc aaaaagtgta tcttcaagac
    12781 attcttcagg aaaaaaactt tatgaagtat gcgatttagt gatagataat catggtcaaa
    12841 agggagatgc ctgtgtagag atagatggat taaaccaaaa agttagtcca acttctactg
    12901 taatagcaac tactgtgatg aactctatta tagctcaagc tactcaggaa ttagttaata
    12961 aagggctaaa gaatcctccg attttctata gtgcaaatat tgatggtgga gatgagttaa
    13021 ataagaagat ttttgatgaa tataaaagtg taattcatta tgaatattaa ggttaaaaaa
    13081 atatgttaat gaactttgaa gtttatcaac aaagaatatt aataatatat aataaatgct
    13141 atcatttaaa agctgtaggt aaaaatctac agctttttat tattgtagat taaaaagatg
    13201 cgtaattaaa ttttaaattg gagaaaataa attggaaggt atcaattata aatattagaa
    13261 acgttgtcat agaaaaaaac tatattaaaa aaaagtacat agaaaaaact gattagacaa
    13321 taaatacatg ctttgataat attttaaatg gagtgaaaat gtgtagaatt ataatgaaaa
    13381 aagaaaataa aaatgaaaaa agaaaataaa atggagggaa taaaagtggg tatgttaaaa
    13441 aaattatgtt cactagctat ggtagcaatt atgactacaa cacttataac agggtgttca
    13501 agtggaaatg gaaaagattc aaaaaaggat ggcgaaaaag aaaaactagt tctttggatg
    13561 ccaccagtag aaaataatat gaaggaagtt tgggacccta ttttagataa atttgaggaa
    13621 aaaaataact gtgaagtaga tttacagata attccttggg ataattattc tgaaaaattt
    13681 gcaacagcta taagtgctgg cgaaggtcca gatgtaggat atatgtatgc agaaatgtat
    13741 ccacagttta tagaaagtgg agcagtagaa gatttgacta actatgcaac taaagaggat
    13801 aaagagcaat ctatttatat aaaaacatct gaaatgatgg gtggaatgta tggtatgcca
    13861 ttccaagcag caaatccagg ggtattatac tataacaagg atattttaga gaaactagga
    13921 gaaaaacctc ctaaaacatg ggaagatttt aaaagaattt gtaaaaaagc aacgcaagat
    13981 actgatggtg atggaaagat agaccaatgg ggattagctc agggatgggg agctaaaact
    14041 tttggtaact taaactggaa ctggtatcct tatttatggc aagctggtgg agatatattt
    14101 aatgatgatt taaaatcggt taaatttaat gataagagtg gtttagaagc agctaagttc
    14161 ttaaaagaat tacaagcata tgtgcctgaa gattctttat ctaaagatag taatgaaatg
    14221 atagaaagtg tatttggacc agggaaagca gcatttacaa taatgttatc ttctgctgca
    14281 acaagtgtat ttgatgaatc attcccggat ttaaattggg gatttgtaac tggactagaa
    14341 gataagaaag cagcaacttt tggagcagta gaccatttat ctttaatctc atctgcgaaa
    14401 aataaagaac ttgcatacaa gttaatacaa catatgttaa gtgttgaatc aatgacagaa
    14461 tttcataaag caataccaag agcaccaata acaaaaggtg aaccatatca aggtgatgaa
    14521 agattcaaag aaatggtgga aaatgacaaa aatgtatata gaccattagt agtaggacct
    14581 catggagttg aaatatatga atatttatgg aaagaacttc aaactatgat ttcaggagat
    14641 aaaactccaa aacaagcgtt agatgatgct gcaaaatatt caaatgattt attagcacaa
    14701 taaaagttta aagctgtaat atgttgtaaa gtactttaca gattaaaatt agcattgtat
    14761 ttataaatac aatgctaata tttatattgg agaggataaa atggaaaact tagttagaga
    14821 gtttgaagaa aattcaaaaa gaaaaaaaac aaaattaaat attaaaccat atatgtatat
    14881 attgccattg ggaataattt tagtggcgtt ttatgtgata ccaataataa tgtcaatata
    14941 ctttagtttt acaaagtata atattattag tccagctact tttataggtt tagaaaatta
    15001 taaaaaatta tttacagatg aaacattaaa agtatctatt ataaatacta ttaaatttac
    15061 tgttgttgta gtaccttgcc aaactatatt atcattgatt ttggctgttt ggattacagg
    15121 aaaaggaaat agtaaaatag cttcatttgc taaaggtgct atatttatac cagtattatc
    15181 atctatggtt ttgataggta tggtatggag ggctttattg aatggagaag gttctattat
    15241 atatcaggta ttaggtgcct ttggaataga atcatctaaa cttttaggag atagtaagac
    15301 agcattacct acacttatgt ttatatctat gtggaaaaat atagggtatt tcatggtaat
    15361 ttatatttca gctataatga atttaccaaa acactgctat gaagtagcga aggttgatgg
    15421 ggctacaaaa cttcaagaat ttataaaaat aactgtacct ttattgaaac caactacaat
    15481 aatggttgta tttttagggt cgatatggtc attacaagta tttgacttaa tttatactgt
    15541 gacaggtgga ggtccaggta tatctactat gagtatagtt atgcatgcat ttaacttaaa
    15601 ctttaaaaac tttaattctg gatatgcaat gactgtagca aacgtattat ttttgttaat
    15661 agcagtagtt tcgattttac aaaataagct tgtaaaacga gacaattcag atttttagaa
    15721 aggtaggtat aatatgaaaa cagcaagaag attattaagt gatggtttac ttttatttat
    15781 agcgtgtgca agtttggtac cttttatata catgttgata atatcattga agataacata
    15841 taactcatat agtttggata tatctttttc aacagtaacc cttcaaaact atatagatat
    15901 ttttactaaa aaaggatttg cacaatattt ttttaataca gcaatagtat ctttttcagg
    15961 tgtattatta aatttagtat ttagtacatt agcaggatat tcttttgcga agatggattt
    16021 taaaggtagt gataagttat ttttatttat gataatgact ttaattatac cttcccaagt
    16081 tacaatgata ccactatata taataatgaa acacttagga tggataaata gttatcttgc
    16141 acttattatg ccaataccaa ctgcatttgg cgtatttata atgagacaag ctattttagg
    16201 agtaccaaaa gagttattag aatcagcaaa gatagatggt tgttcggatt ttagaatttt
    16261 aatacaaata gtgttgcctt taattaaacc agcattaatt acactagcaa tatttacttt
    16321 tatgggagca tggaacgagt ttatgtggcc tcttatagct acaactaaag atgcaatgag
    16381 aacattaaca gttggattat ctactcttaa gacattccag ataactaatt atggacagat
    16441 gatggcaggg gctacaataa catttttacc tccatttatt ttctatttga ttttacagag
    16501 taaatttgtt gaaggtgtgt ctttgtctgg tataaaaggt taaatagtct aatttaataa
    16561 tatataaaga aacttatata aatatggagg aatttaaact atggaaattt catatgtact
    16621 gaaagattgc acattcaaac acaataaata ctcagttgta gaagctgaaa cttggaaaga
    16681 taaggatttg gatattgtag tagctaaagg tgaaaaattt gcatttcaaa ttatgttaaa
    16741 agcaacagaa gaatttaatt gtacaattga taaaagtagt gctatatcat ggaaaggcct
    16801 gggaaataga gtaaggttag cattaaatat gcctaatagt ttagaaaata attttagtat
    16861 aaatatatta ggatatgttc aagatgatac aaaggcattt gtgaatgatg ctattttaag
    16921 ggataaagat gtgttagtag agcaatattt gcctcaaaca ttttggattg aaggtcaagt
    16981 accagaagat tttgaagaaa ataaccttga tataggtatt gatatattta aaagctttgg
    17041 atatgaagat gaagaaaaag tttgtacaat tgatgttcct gtaaaagtaa gaaatgttgt
    17101 gctaaagcct ttagatgaaa gcaagttctt tttagattta tggcaacatc caagctgttt
    17161 agctagaatg tataaagttg agttatggtc tgatatacat tttgaaatag tcgataacta
    17221 tttaaaagaa ttagcatctt taggagaaaa agttgcaaca gttatagttt cagattatcc
    17281 atgggcaggg caaagttgtt ataaagttta taaaaatcca tcaaacttat atgaatataa
    17341 catggtaagt gtatcaaaag gattggatgg aaagattaaa tgtaactttg agagtatgga
    17401 taggtatata ggcatagctg ataagtataa aatggctaag gagatagatt tatttggtct
    17461 attaggtaat tggtgtgctg gtgagtttgg aaatcctgta gaaggttata aggaccctat
    17521 aagagtaaga tattttgatg aaaaggataa agtatttaaa tttataaaca atacaaatga
    17581 tttaaaagaa tatataggat tagtattaaa tcatttaata gagtgtggat tatgggacag
    17641 agtaaggatt atagctgatg aaccaaataa tccagaagta gtcaaagagt gtatagaatt
    17701 tataaattct actgtaggta ctcatcaagt taaatataaa tcagcaactc acgaccaaaa
    17761 ctttttagac agagctaaag atgaaattga tgatatgtca ataaatttaa aacttactat
    17821 acaaaattat aaagatatag aaagtcttaa gaaaaaaatc aatgacaagg gtgggatatt
    17881 gacttggttt gtatgctgtt ttccagaaaa accaaatagt tttttaagtt ctccttttgt
    17941 agaaaataga ataataggat ggtatactta ttactttggt cttgatggat ttttaagatg
    18001 ggactataat ttatggacag aagacccttg gaaagattca agttacaaat tccctatttg
    18061 gaaagctgga gatatgttct ttgtttatcc tggaaaagat ttaaagccag ttagaagtgt
    18121 cagaatggag aatttaagat ttggaataca ggattttgag ttatttacaa tgttagaaaa
    18181 agaaaaggta agagagtata tagaagttga gcttatgcaa gaattattaa ataaaaaaga
    18241 aaatgctgaa attaaaggtt ttggtgacat agaactagga tattcacttg ataattggag
    18301 atatgataaa gttaaaaaat atgtattaga gttgttagac agagtataaa aaatataaga
    18361 gtgtctcaaa atgaactttt tagttcatga ggcactcttt ttgtatgaaa tcaatatagt
    18421 ttcattgttt caacaatgaa aaataggatt atttctattc taagacagcc ctttagtttt
    18481 ttagttgtat tcaatgtttt aaaactaact ataaacgcta taaagtttat aaatgacaat
    18541 aattatatat aacatgatgt actttaattt gctataatag ggattgaata atattatatg
    18601 gaagataaca ttgtttgggg ggattaatat aaaaagaata ggtattatta taatgttttt
    18661 tttaagtata tcttttattg gattttatct tgtaataaga gataacagtg atacagttaa
    18721 gatgcaacga gggataaata taggtaatgc actagaatca cctaaggatt ttccgtggga
    18781 tgtaaaaatg tcaaataagt tttttgatga tataaaagat gcaggttttg acacagtacg
    18841 tataccagta aggttttctg attatacgtc agattctgat aattttaaaa ttgatgaaga
    18901 attttttaaa aagattgata aatatgtaga ttatgcattg gataaagact taatagttgt
    18961 gttggattta caccattttg aagaaattat gaaagaacca agagttcata aagaaaagtt
    19021 tttaaaaata tggcaacaga tagctaatag gtatcaaaaa tatgataaaa aattggtatt
    19081 tgaattatta aatgaaccta aagaaaattt atcttcacaa ttactaaacg aatacattga
    19141 agaagcaata aagataatta gaaaaactaa ccccaaaaga actattatag taggaccata
    19201 taatttctat caaattgatt atttgaatga attaaatatt cctaaagatt ctaatatagt
    19261 agtttctttt cactattacg agccaaatga ttttgcattt caaggaaata tctatcataa
    19321 gggatttgag cacttaagca atattacttg ggaaggaaca aatgaacaaa tggattattt
    19381 aaaaaaaagg tttgatactg tggaaaattg ggcaaataaa aataaggtaa aaatcttctt
    19441 gggtgaattt ggaataacaa aagaggcacc agaagtttca agaagagcat ggataaaagc
    19501 tgtaagagaa gaagctgaaa aacgaaactt ttcatgggct tattgggagc ttgcatctgg
    19561 atttggtata tacaatcaaa ttgaagggac ttgggataga gatatattaa gtgcattaat
    19621 agaaaagagg tgattttatg aagaaaaatt caaaaaagat acttataact ttaactatta
    19681 ttactaatgt catatatata ttgtggagaa ttttttacac agtacctaaa gaagaaggga
    19741 agtttgcctt aatatgtgcc ataatacttc tttttgtaga gataatgggt atgatggaaa
    19801 tgtttgtgca ttattatggt atgtcaaata ttgagtatcc tgaaaaacca atcataagtg
    19861 aagaacttta tccacatgta gatgtattta ttgcaacata taatgaatct gttgatttgg
    19921 ttagaaaaac tgtaaatgga tgtattcata tgcagtatcc agataagaaa aaggtacata
    19981 tatacatatg tgatgatgga aatcgtgaag aaatgcgtat tctagcagaa aaaatgggtg
    20041 taaattacat aactagaact gaaagagaag gagcaaaagc tggaaattta aataatgcaa
    20101 tgcaacatac aacttctcct ttgattgcta catttgatgc agatatgata ccaatgcatg
    20161 actttttaat tgctacagta ccatactttt taaaaaatga gcaagctaaa aaagatggag
    20221 aaaaagagga gtatgaaaag gttggttttg tgcaaactcc acagagtttt tataatccag
    20281 atttgttcca atttaatctg cactctgaag gaaggattcc aaatgagcaa gattattttt
    20341 acagagatat tcaattggct agaaatagga caaattctgt aatttatggt ggtagtaata
    20401 cagttatttc aagggaagca cttgaagaag ttgatggatt ttatacttat tctattacag
    20461 aagattttgc aacaggtatc ttaattcaaa gtaaggggta tagatgttat gctattccag
    20521 aagtacatgc atcaggttta tctcctacag atttaaaaag tcttattaag caacgtgaaa
    20581 gatgggcacg tggatgtata caaactggta gacgtctaaa tattttgttt aggagaggtt
    20641 taggtttttg gcagaaaata agttatattt cttcaattac ttattggtat gcaagtataa
    20701 aacgctttgt atatataatg gcacctattt tattttctgt gtttaatgtg attgtagtaa
    20761 aatgtacgtt acttcaagta ttggtatttt ggttgccaat gtatatttta agtagtttat
    20821 ctcttaaaat attttctcaa aatataagaa atactagatg gaccaatata tatgaaacta
    20881 taatgtttca gtctttaatg ccagcagtaa tacttgaaac gtttgcaata tctaaaaata
    20941 aattttcagt aacaaataag agtaaattag aagaaaatag aatgtataag tttttacagg
    21001 gaatacctta ttttatttac atggtcttat ctatcatagg aattttaaaa atgtttgtag
    21061 caatatttaa aatgagttca atgacatatt cagttgtatt attttggttg attggaaacc
    21121 tttttaacct tgttatggct acattattta tatcagggag acagcagtta agaaagtctg
    21181 agcgttatat tgctgaaatt gattttaagt taaagcaaaa ttcttatgtt ctttcttcaa
    21241 aaacaattga tatatcagaa aatgggtttg catttttgct agaaaatcca gaatatattt
    21301 caccagaaga agagtttgag gtagagttta gagaaaaatc tggaaatgaa atgtatatag
    21361 ccaatatgaa agcaaagatt gttaatgttg tggaagtaaa ttcaaagtgg aaatatgcag
    21421 cttatattac acatattgaa gatagtgaaa tagataattg gatgtgtatt gttcacgata
    21481 gaatacctac tttaccaatg acaatatcta atcaactagg tttctttgat gatttgcaaa
    21541 ttaatgtgaa aaaacgtatt gaaaaaacta gaacattatc acgaagaagt ccaagaatca
    21601 atatgaattt tcagatggat ataaaaaata caggtaaatt aagaatagtg aattttaatt
    21661 atcaatatgt attattaaat tttgagaata aaagtattta tcctaaagaa atagcattgg
    21721 aaattaatga aaatattgtg ttagaatgtg atttgtgtga gggtaaaatt gatgaaagag
    21781 gtatcttata taaagtaaat aacattgaca gcataatgca aaatttcttt ttaagagatg
    21841 aaatgatgga ttggatatta caaaataaga caattcttgt ttcaaagcct agtgaaaaga
    21901 aggaaaaaag tatagatgag tttgaaccca tggaatatat ttaggagaga tgaaatgaag
    21961 actttaaaag tgtttttttg ttctacaaaa tatcttattt tggtgtttat tttttttata
    22021 aatactactt ttataagttc agcaacaagt gataaaattt tagatttgtc ctttaaaaag
    22081 atagagactg acttaaattc taagattaca tatgaagata caggtgtaaa aatagaaacg
    22141 gatagttcta aaagcgataa ggaacgttat ttatatattt accaaaatat aaaagaaaat
    22201 tggagcatgt ataataattt ttatattgaa attcaaaata aaaataaaag ttctcagaga
    22261 ataaatttaa gcatacaaag taaaaatatg cttgaattta gattaaaaga aggtagtgaa
    22321 gtatttttag aaggtaaaaa tataatatat tcagataaaa taaaagaagg atgcatagaa
    22381 gtaccaggtg aatttgaagg caaaatttat gtgaacttta atagccttat aaatgaggaa
    22441 agtaatgttg tgctggactc aaatatgctt tcaaacattg ttagttgggg aattacgttt
    22501 attccatctg atgaagaaca taatattgtt attataaaaa agatatcttt attgtctgaa
    22561 gagaaattaa gatttttaaa taatattaaa ataattggcg atgaagaagt acaaatacca
    22621 gtattgggtc aaagtataag tcagtatgaa gtattaggtc ttaaaagtga ttctaaaatt
    22681 aagtactctc ttatgggaaa acaagataat gtaagcatat ctcaaaaagg taaattaaca
    22741 ttaagtaata aagccaaacc tggacaaatt attttacaag ttaatgttga tgataagttt
    22801 aaaattggaa agaaaataac attaactgag tcttggagtg ttaataaaaa agataaggat
    22861 ggtgttccat atacattagt atatcctgag caatcaccaa cagtacaaga tatgaaaaaa
    22921 ataaacttta tgaataacat cataacgttt gttcgaattt tatttgtatc tctagtaata
    22981 atatgctttg gaatatattt atattggaaa aaatgttcaa aaactaagta aagagaggag
    23041 gttagattgt gttattttca agtttaattt ttttatttta ttttttacct ataactttgg
    23101 ttttatatta tgtatttagg tttaaccgaa cgatacagaa catgatattg cttgctgtta
    23161 gtttattttt ttatgcttgg ggagagccaa aatttgttgt tatcatgata gtttccataa
    23221 taatgaacta tatatttggt ttactggtag atagatatag ggaaagtaaa ataaaagtaa
    23281 aagttttttt agttttgatg tgtgcataca atataggtgt tttatttata tttaaatatc
    23341 tagcttttgc gctaagaaat attagtgaac ttattagtac tgaattgact atacctagta
    23401 ttgttttacc cataggaatt tcatttttta catttcaggg aatgtcttat gttatagatg
    23461 tatatcgtag acatggagaa gtccaaaaga acccttttta tgtgggattg tatatagcat
    23521 ttttcccaca attaatagca ggtcctatag ttagatatga gagtgtggca gaacaaattc
    23581 tcaatagaaa agaaacttgg aataaatttt ctattgggac ttgtaggttt ataacaggtc
    23641 taggtaaaaa agtattaatt tcaaataaca tggctatagt agcagactat atttacacta
    23701 tgaatagtca aggagaaata gctgcatcac ttgcatggct tggttcaata gcctatactc
    23761 tgcaaatatt ctttgatttt tctgcttatt cagatatggc tataggctta ggtcttatgt
    23821 ttggttttaa atttgaagag aactttaatt atccatatat atcaaaatca attagtgagt
    23881 tttggagaag atggcacatt tctttgggaa tgtggtttaa gagctatata tactttccgc
    23941 ttggaggttc tagagtagca aataaagata ttatgattag aaatatgttt atagtgtggt
    24001 tatttactgg aatatggcat ggagcagaat ggacatttgt aatttggggg attttaaact
    24061 ttgttttcct aattatagag cgatttataa tgtttgaaaa gattgaaaat cacaatttta
    24121 taaaacatat ttatgcttta ttagtagtta attttgggtg ggtattattt aggtcaccta
    24181 atttaaaaga agcatataat tattttaaag ctatgtttgg aggcaatgga gctatatgga
    24241 gtgattatac atatatgttt ttaaaagaat actgggtttt ctttatattt gcatttattt
    24301 ttagtgtacc tattgcaaag aaaatcaata agtttgttgt tgaacaagct aaatatagta
    24361 tgatatttaa cgtattttat ccaatctcag ctattttatt gttttttatt tctgtgacat
    24421 atttagtaac tggaagctat aatccattta tttattttaa tttttagttg ggaggaaaag
    24481 tatgaataga ttttttaaag taagattaat aactgcaatt atgttcttaa ttattttatt
    24541 cgctttctct atctttaatg tttgttattc tggaaaagat acgatacagg aaataaaaaa
    24601 ttcaatagct gaaaaaagta gtttaaaaga tacaatatca tctattgata atacagtgaa
    24661 tgacaaagta atatacaaaa atacgttcat tgaaacttat ggctatttac aaaaattaat
    24721 gggtaaaaat gagttttcaa aatttagtgt tgtaaaagat acttctggaa agatgcatta
    24781 tacatatttt gctaaagctc caaatccagt tggtgtgata gcagataggg taagaaaatt
    24841 ttctgatgaa atggaaaagc aaaacactaa attggtttac ctaatgactc cagataagta
    24901 tataaaagga gtgacaaaat ttccaaaagg aataccttat tcttatagta atgagacagc
    24961 agataatttt ttaaatcaac taaaaaagta taatgtggac tatattgatt ttagagaaaa
    25021 tattttaaag agtggtattc caaaagatga tttatttttt aaaacagacc atcactggaa
    25081 agtagaaact tctttttggg catttggaga attagttgag caacttaata aaaaatacaa
    25141 tatgaattta gatgaaaatc attattatag agataaggac aattataatt caattgtata
    25201 tcctaagtca tttcttggtt ctatgggaag aaagactggt atcttatatg gtggtataga
    25261 tgattttaca ttaatttatc ctaaatttaa gactaattat acttattata cagattctaa
    25321 atctcaaaaa tttgaattaa aaggaaggtt tgaagagagt ttaatattat catatccttt
    25381 taatgcagac cttgatttaa tggatggtca atcagataag tattttacat atttattggg
    25441 gaacagacca ttagttaaaa taaaaaatat agaaaatcca gatggattaa aagtgctgtt
    25501 tgttaaagat tctctaattg taccaacagc agcatttttt tcctctgttt gttcatctat
    25561 agatatgata gaccctagat attatgatgg agatatacta gaatatgcaa aatcccatga
    25621 ctatgacttt gtttttatat ctgtatatcc ccaaaatctt acaaaagaat tttttccgtt
    25681 ttgtgagtaa atttctaaaa aagttttctg ataaaataaa tggctaactg gtaaaaagtt
    25741 agccatttat tttgagtgtt attctaagaa atattttttt tactaaatac ttctttatct
    25801 atttttccaa gtgattttcc tacagtaatt gatgatacca tagcaccatt aacatttatc
    25861 atagtacgtc ccatatctag tatagggtct attgcaagta taccaccaac taatggaaaa
    25921 taagaaccaa gaccaactcc agagataaca actgaaacgg ccattgtagc agttcctgga
    25981 agaccagcta ttccaaacga actaattgtt ataacaatta acaacataac ataaaagctc
    26041 atatccatgt ttactccagc catattggca agtgttacag ccattaaagc aggatatata
    26101 cctgcacaac cattcatacc catatttgca cctaaacttg ctgtaaagct tgatacaccc
    26161 tcatcaactc cgtgattttg tactagactt tctatcgtta caggcaatgt tcctaaacta
    26221 gaacgtgaag taaatgccag aagtaaagct tcagttgaat tttttatata agtaatggga
    26281 tttagaccat ttaaagttat aaaaattaaa tgtataataa ataatattgc aactgatata
    26341 taaagagcta atataaactg taaaacagat actattgaac taataccttg tgaagttatt
    26401 gtatttgcaa gcagagcaac cacagcataa ggcataaatt taataacagt catagacaca
    26461 cttacaatta ttttgtaaga tgcttctata aaacctataa atggttttat tgtttcaaaa
    26521 tgttttttac ttaatctctt tattgaggta gctataaatg tagcaaatat tattgtagct
    26581 acaatatttc catttgccat agccattaca ggattagatg gtagtaatcc tcttaaagta
    26641 tccacaagag agtttatttc tttaagttct tgattttcaa ctaaagtagg catattagct
    26701 cctacaccta acttcattat atttgcaacg ataattccaa caattgcagc tatagcagta
    26761 gtagcaagaa gcatacctaa agatttaaat gtcagagttc caagattgtt atctttcata
    26821 tttataatta cttttataat ggataagaaa actaaaggta ctactaacat ttttaataag
    26881 tccataaatc cagaaccaaa taggctatac cacttactta cttcttgtag ccaaatgact
    26941 tgagttgtat cattaggaaa accagcaaaa gcttgtatta tgattcccaa tatgagtcct
    27001 agtactgtag catatattgt tctcaatgaa aattttattt ttttcttttc aaattgtcta
    27061 acaataaaga acgtacctag aagtaatatt atgaaaaaaa ttgttttaaa atctgtaatc
    27121 ataagaaatt gtgataaaaa tgaactttcc aatatttttc tccttttatt taaaaataaa
    27181 tttttctaag tttaaatgat atcaatatat taaaaaaatg tcaatattat atcacaattt
    27241 acttaaatag agtaaaacaa gttgattttg aaaaatgtga ttaagtacag aaaaataggg
    27301 tataataaat gtttaaagaa tttgaattgt ggtaagaaaa taaaaaatag cttttataag
    27361 atttgatttc aaattaaaat gtgttaaata aataactaaa aattattata aaaaatgttt
    27421 tcatagaata tatatcaaat gaaaagctta aaataagtga agaaattaac tagacatcaa
    27481 aggagagata aaatgagagt aataataatt ggtggtgttg cagctggtat gagtgctgca
    27541 gcaaaattaa aaagaataaa acctgagtat gaagttgttg tttatgaaaa aactgaaata
    27601 gtatcttttg gtgcttgtgg attaccatac tttgttggtg gattttttaa tgatgctgat
    27661 gaattattag caagaacacc agagaaactt agagaagctg gaattgattt aaatatattt
    27721 agagaagtcg ttgaggttga tagtgaatct aagaaaataa aagtaaaaaa cataaaaaca
    27781 gatgaaatat atgaagatta ctatgataaa ttgatgatag caactggagc aagaagtatt
    27841 atgcctccta taaaaaacat aaaattaaag aatgtttcta cattaaaaag tttatatgat
    27901 ggagaatatt taaaaaaact tttaagtaat gaagataata aaagagtgac aataatagga
    27961 gctggtttta tagggttaga agcagttgag gcatgtaaga aattaggtaa agatgtagat
    28021 gtaattcagt tagaagatag aatattgcca caagtatttg ataaggaaat gacggatgta
    28081 ttagaagaag aaataaatag acataatgtt aatttgcact tagatgaaat ggttgtagaa
    28141 ctttgtggag aagataaagt agaaaaagtt ataacaaaca aaggggaaat agatacagat
    28201 gtagtaataa ttgctactgg cgtgagacct aataccgagt ttttgtcaaa tactaatata
    28261 aaaatgctaa aaaatggagc tatagtagtt gatgagtatg gaagaacttc tgtagaagat
    28321 atatactctg ctggagattg tgcaactata aagaatatag taagtaatga aaatgtatat
    28381 gttcctttag ctactggagc taataaattg ggtaggatag taggagaaaa cctagcagga
    28441 agagaaatat cttatcaagg ttcactatct tcaagctgta taaaaattat ggatatggag
    28501 gcagcatcaa ctggaattac agaaaggcaa gctaaagatt taggcataaa tgtcaaatct
    28561 aaatttattt ctgattataa tcaaactcat tattatccag gaagaaataa aatatatgta
    28621 aagcttattt atgatgcaga tacaaaagtt attttaggcg gacaagtggc tgggtttaag
    28681 gatgctgtcc aaagagcaaa tgtattggca gctgctatct ttggaaaaat gaccacaaat
    28741 caacttggaa tgttagattt atgttatgct ccaccatttg caagaacatg ggatgtttta
    28801 aatgtagctg gaaatgtatc taaataatat ttagattata gctaatatag ttgacaagca
    28861 attaagggaa tgaatttacc taaactattt ttatcatggt aaaattcatt cctaaatttt
    28921 tattccaaat ttttattcca aatttttaga aataaggatt ccattaaatt attcatcaaa
    28981 atttaaacgc aagcttaatc tatcttctcc aagaatagta ttttcaaaaa cattagtagc
    29041 attttctaaa tacatactag gtatgtctaa agaaggacta aaatgagtca gtaaaagttg
    29101 tttgacattt ccaagttttg caagattagc agcttctcta aaagtcatat gtttgtttct
    29161 aacagctttt gatatatcta aatcatcacc atacatggct tcacatataa ataaatcact
    29221 atcttttatg aaatctggta ttgattctat aggtcttgta tcggttgtaa gacttatctt
    29281 aactcccttt ctcaattctc ctaagaccat atctggagtg tattgttttg aatctaaaac
    29341 tatattttga ccttcttgga gtttgttcca taatatttta ggaacttcgt ttttagttgc
    29401 cttatctata tcaaatttag ggtttcttct aaaattaaaa ctgtatccta aacactctat
    29461 agagtgttct aaactgattg ttgaaatttc taaatttctt aaaactttat tatctaaaga
    29521 aaaattgcct tgagggtttt ctataatttt taaagtgtat ggtacatatt ctactaaatt
    29581 tctcatagaa cgtatacaat cgactattcc aactggacca actatagtta aatcacttgt
    29641 acgaccacta ttcccaattg ttgataaaag tcctaaaagt ccaaatatat ggtcaccatg
    29701 taaatgggtt atacaaatca agtctatatc tttaaagcca cattttttta gcttcataga
    29761 tacctgagta ccttcaccac agtcaattag tatttttcta cctttatagt ttataaatac
    29821 agaggataga aatctgtttg gcataggtac atttccacca catccaagta aagttaaatc
    29881 tatcattttt tcccctttct aaaattaaca caaatctgtt gtttgttttt attatgatat
    29941 cctattataa ttattttaac attaaatata acaataaatg tgtatttgag tcaagaaata
    30001 ttgtcaaata aagtttttat atagcaatgt ataaaaattt ggaaaacgaa aagtgaaaat
    30061 tatatttagg agtatttaaa ggaggaaaat ttaatgaaag tattaaagaa aattactatt
    30121 ttgtcattaa ttacattaac cttagttggt tgtagtaacg gagaagagaa aaaagtggtt
    30181 gaaggttcta agaaaactca aaattctaaa aaagatgaac agcctataat agtacataaa
    30241 gatgcaaata tattgaaaga taggtatgag ccaccaaaag gatttaaaag agttgatgta
    30301 gaaaaaggta gttttgaatc atttcttagg aatactaagc taaaaaatta tggagagaaa
    30361 gtaaagtatt atgatggaag aactaaggaa tcaaaaggta tttatgatag tgtatttgat
    30421 gttgatattg gaaatagaga tttacatcaa tgtgcagatg caattatgct tatgagagca
    30481 gaatacttgt atcagaataa actatatgat gaaataagtt ttgattttgt tgatggtttt
    30541 aaggcgaagt attcaaaatg ggcacaagga tatagaattt cagtaaaaga tagtggttca
    30601 agctggtata aagcaactga atattcaaca tcttatgaga gtttcagaaa gtttatggat
    30661 atagtgtttg catattcagg aacgttatct cttgaaaaag aattggagcc tgtaaaagtt
    30721 gaggatatga aaataggaga tgtgtttata gttggtggaa gtccaggaca tgcaagtata
    30781 attatggata tggcagaaaa tgaaaagact ggtgaaaagg tatttatgat agcccaaagc
    30841 tatatgcctg cacaacaaac gcaacttctt attaatcgta aaaatccaaa tttgagtcct
    30901 tggtatagtt tagattttga aggtgatttg acaatacctc aatggacttt taaaagagac
    30961 cagttgaaaa gattttaaaa taataatttt atataataaa agaaaaatat agatgtgaaa
    31021 aaatgtaatt agattaaaac taattggata tggacaaaat taaattgata gataaatatt
    31081 aaaaattaca ctaattaatc ttactatagt ttaatattta tctaaaaagt tttatgtaaa
    31141 atataaattt tatattaaag ctaaaactaa tttgaggagg ctaatttata ttgaagatta
    31201 gaaggtattt aattttggta attataattc taattacaac aagtcagagt tttatttctt
    31261 atgggctaga gcgatttgaa gaaataacca tttttcacac taatgatata catggaagat
    31321 atgctgaagg tgatgaccat atacaaatag gaaatctagc cacattgaaa aaagaaactc
    31381 caaactcaat tttagtagat gcaggagatt gtttgcatgg acttccaata gtaaatatgg
    31441 atagagggaa aagtgcaatt gaattaatta aatgtgctgg atatgactat attactcctg
    31501 gaaatcatga ctttaattat ggaaaagata gactgtttga gttatctaaa agtgctaatt
    31561 taggagagaa tactttaaaa tttttatcat caaatatttt tgaaaatggg aaaaaagttt
    31621 ttgatagtaa tgatataaaa gaaatagatg gaataaaaat aggatttttt ggactctcaa
    31681 cccaggaaac aaagagcaaa actggattta aaaatgtgga aggattagag tttaaaaatc
    31741 ctatagaaag tgcaacagaa caagtaaatg aattaaaagg aaagggtgcc aatgtaatag
    31801 ttgcaatatg tcatattggt acaaatagtt taagtaagcc aaatagtata gatatagcaa
    31861 atgaggttca aggaattgat ttgattattg atggacacag ccacactaag tttgaaaatg
    31921 gtaaacaagt gggtgataca ttgattgtaa gtgcaggaca atatttagaa aatattggcg
    31981 agataaaact aactttggat actaatgatg tagaagatat aaaaatagaa aataaatcag
    32041 ctagattaat tggaaaagaa gaagcattaa agtataagct tgactccgtt gtaagtaaaa
    32101 aggttgataa aataaaagaa caacaagaaa aaatattaga taaagttata ggggtaacta
    32161 caaatactct agatggttca tatgaaaatg taaggacaaa agaaactaat ttgggtaatt
    32221 taataagtga cattttacta gataaaacta aggctgatat ttctttattt aatggaggga
    32281 atattcgaga tacgatagaa aaaggcaata taacaagaag agatattgta gatgtatttc
    32341 cttttagcaa tactatagtt accaaagaac ttacaggagc acagattaaa gatgtattgg
    32401 agcatggagt aaaactttat ccagaaaaaa gttcagcctt tttgcaagtg ggaggaattt
    32461 catattactt taatcctaaa caaaaagaag gcgaaaaaat aacaaatatt caaaaagaag
    32521 gaaagccatt agatttaaat aaaaagtatg tagttgcaac gaatgattat atagcatcag
    32581 gtggagatga atttccttgt ttttcaaaag agccagtact aaaggaattt ggaaatttag
    32641 agagtgcagt cattgaatat atagagtaca aaaaagaaat atcaaaaaat gtagatggaa
    32701 gaattggtat taaatctaaa gatgagttaa taagcaagaa tcatgagagt gatttgatat
    32761 taagtgaaaa tgaaaaaaat atcaatgata atgaagagaa tctatctaat aatataaaaa
    32821 gctctgatag tgataaaaat aaaagcttag ataaaacaag ttctagagaa agttctaatc
    32881 taggaatatc taaaagagaa gctaaagaaa aatctcctaa aacaggagat ttaggttttt
    32941 caaatagtat aattatattt atagtttcaa gtacattgat atgtttattg aattttaatc
    33001 aaaaagagtt aaaggataaa aagtcaaaat aaaaattaat gagcaaaaat tagtagatag
    33061 taatttaatt ttagataata gtaaacagca aaggaaatta gttatataaa tactaatttc
    33121 ctttgtttta tgttttaaca tgattacttt taaataaaat tgtattttat gaaaaataat
    33181 tatactaaat ggcatagtat tatggggatt tttagttttt attcaagtaa atgaaatcat
    33241 attaacaact tctgatatat ttacaaattg ataaaaaaat gattttatta gtatgatata
    33301 atttagctag tatgtatgat aatggaggat atagggatga ataatatatt acttttggaa
    33361 gatgacaaat ctttaaatag aggaattagt tttaaattaa aaaaagaagg atacaatgtt
    33421 tttagtgcat ttggtataga agaagcaaaa aatattttcg caaaagaaga gatatgctta
    33481 attatttccg atataggtct acctgatggt agtggttttg acttttgtga ggagattaga
    33541 aagaaaagtg atgtttacat aataatgttg acagctcttg atgaagaggt ggatattgtc
    33601 acaggatatg atttaggtgc agatgattat atcacaaagc cttttagctt gatggtatta
    33661 atatcaaaag tgaatgcgct tatgaaaaga gtaaatacag taaaaaatta tactctatta
    33721 gtttgtgatg atttattctt ttattatata gaaaataaat taattataag aacagatgat
    33781 aaggaagaag agataatttt aagtaagaca gagacaaaac tattaaaata tttgatggaa
    33841 aattctatgc agacattgac taaagagcaa ctgttagaat ctttatggga tagtagtggt
    33901 aattttgtag atgataatac gattgcagta aatataagaa gacttagaca aaaggttgaa
    33961 aaaaatccat ctgaacctaa atatataaaa acagtaagag gggttggata tatgtgggga
    34021 gaaaggagta taaaaaaatg ctaaaaaata tgaacctcaa agatggttta agtgtgaaag
    34081 atgattatat aatgagaaga gttattgtat attctgtttt atctgtacta atagtaaccc
    34141 ttgtatttat actttttgaa tatagcagat tacataaatt gtatgaacat caaataaaaa
    34201 atactcaaaa tgttgctgga atattgattg aaaaatatcc taatgatgaa atagatatag
    34261 taaattctat ttataattct gattatagta aggtgtctac tggagaaaag gcatttaaga
    34321 aatttggcta tggattggaa aataaaatgt caaatgataa aaattttagc ttatacttaa
    34381 aatctttttt taaggaatca ttttttattt ttttaatgat gtttatagta gttataggta
    34441 taatatatta ttttataaga tacataaata aaagattgag taaaatttat tttatagtag
    34501 ataagatgtc acaagaaaac tacttgaagg acactactga gtcagaagaa actggttatc
    34561 tagtagaata taatgaatac tttaaagaag gcatatttag taagataaat aactgtctat
    34621 atgagcttaa tagaagttta aagataaagt ttattaaatt agaaaaagaa aaagaaagtg
    34681 taaaatcatt agtaacagat atttcccatc aattaaaaac tcctttagca tccttaaaat
    34741 tatacaatac attacttata gaagaagaat tagaagaaga tgaagccaac gagttcttat
    34801 taaccaataa ggcttctata aataaattag aaaatttaat aaattcttta gtgaacatat
    34861 caaggttaga aattagcatg ataaacataa aaaaagaaga taatgatata aaaaatacta
    34921 ttctaaatgc agtaaaaagt gttaccccta aggctaagag caaaagtata acattaaatt
    34981 taaatgaatt taacagtaga attataccac atgataaaaa gtggactgag gagagtattt
    35041 ttaatgtatt agacaatgct gtaaagtata cacaggtaaa tggagaaata aatatatctg
    35101 tagaagaaac tgctaatttc tttaaaatta taatagagga taatggcata gggattgaaa
    35161 aaagtgagtt taataatata tttaaacgtt tttatagagg ttctgctgat gaaattgaat
    35221 ctgtagaagg tagtggggta ggattgtatt taagtagaaa gattttagaa gaacaaggtg
    35281 gaaatattat agtttcttca aaagtaggaa tagggagcaa gttttctttg tttcttacaa
    35341 ctatgtaata ttaaatgaaa ggaaaatgta agcctctaca tttattatat aagtataaac
    35401 agctccagaa ataaaaaatt aataaaatat ggagtaatat aacttagaat gagaggataa
    35461 ttttatgaaa atactatata ctgaaaattt atcaaagcat tatggaaaag gagaaagctt
    35521 agtaagagct ttggacaatg ttgacttaga aataaatgaa ggtgaatttg ttgcaataat
    35581 aggaaaatca ggttctggaa aaagtacact tttgcatatg attggaggtc ttgatatacc
    35641 tacatctggg aaggtgtata tagataataa aaatatattt acattaaaag aagaagaact
    35701 tgctgtattt agaagaagaa aaatagggtt tatatttcaa agctataatt taattccatc
    35761 tcttaatgta tgggaaaatg tagtgttgcc aatcggttta gatggtagag aggtagatga
    35821 accttttata aaagagcttt taaaatcatt aggacttgaa aataaacacg acgttttacc
    35881 aaatacatta tcaggtggtc aacaacaaag ggtggcaata gcaagagcat tagctacaag
    35941 accagctata atacttgctg atgaaccaac tggaaaccta gattctaaaa catctgatga
    36001 ggttatgagt atattaaaat ctatgtctaa gaagtacagt caaactctag ttatgataac
    36061 tcatgatgac agtatagccc aaatggctga tagagttata ttcatagaag atggcagggt
    36121 gtcaaaggtt ggtgataaaa atgattaata ctactaaaat agcaaaaagt aatttaaagc
    36181 aaaataaatc aaaaagtatt ctaataataa ttactataat actttcaacc ttaatgcttt
    36241 cttctattgg aatttatatt gttgatgcag gagcatatca aaaagaaaat actataaagt
    36301 attcaggtaa ttatcaaggt atattagcaa atgtagacga aaaacaagct gatatattga
    36361 gtaatcatgc agatgttgaa ttaactggta aaatgaatgg agtaggtgtt gaaaaactgg
    36421 aagatgattc aaatatttct ctagcataca tgaatgaaga tgctttgaaa ttaaatagtt
    36481 ttgaatttat caaaggaaaa ttgccatcaa aagagaatga aattgtgtta gatagtggag
    36541 ctttaaaggc acttggttat aaaaataaag atttaggtga aaagataaaa atatcttata
    36601 atgactataa aagtgataaa aaaattgaaa aagagttcat tataagtgga atcttaaaaa
    36661 ctagcgaaat atctgaagca ggtaaatatt attatgctat tatatcagaa tcatatatga
    36721 gaaatactag agatatgtca caagaagatt ttaatatata tgtgaaatta aatgataaaa
    36781 gtaatttatc gatagaacaa gcaaaatcaa aattaaataa gattgctaat gatgtaggat
    36841 tggacactat taatgtagct gtaaatgaaa actatataaa tgctttaaaa ccagatatgg
    36901 aaacaattat gggtggagta tttgtagggt tagtaattgt attgtctagt attttagtaa
    36961 tatacaatat attttatatt tcaatagtta caaaggttca agaatttgga aagcttagag
    37021 caattggtgc aactaaaaaa caagtaaaaa atatagtatt taaggaaggg ttcatattag
    37081 ccggaatagc aataccaata ggaataattt taggctatgt acttgcaaat attataataa
    37141 aatcatttat ggatatagat gtaaaatcat cacgattacc agttatatta tcggttgtgg
    37201 taataagctt tatatctgtt gttttatcac ttttaaaacc aatgaaagta gcatcaaaag
    37261 tatccattgt tgacgcagta agatatacag gaaataaaat aagtaataag agtaagagaa
    37321 aaggatataa aaatataaat ctaaagagat tatcacatgc caatttagaa agaaataaaa
    37381 aacgtactta catgacattg gcatcactta tacttagtgg aactatattt ataacagtat
    37441 caactgcctt agaaagtttt gatgctgaga aaatggcaag agaacacttt ccttatgata
    37501 tagaagttag actatctggt tatgaaatga acagtgataa aaatcctaaa gataatttga
    37561 atattttaca aatggataat cctctaagta aagatttttt taatcaaata aagaacatgg
    37621 aaggaattaa aagaatagaa tctgcaagaa gtgtaaaaat tggtatggag gactatgatg
    37681 ttgaatttaa gtatgacctt ttacaaagta taaatgaaaa tgatgtaaaa tcattaaaca
    37741 agaatataat agatggaaaa ataaatcttg aaagacttca aactggagat gaaatagtaa
    37801 ttactcatgt tgatacagca aaagaaatgg gagtaaaagc aggagataaa ataagattaa
    37861 ctttatatga tggagataaa aagataaaaa aagaatttaa agttcaagca atagctatgg
    37921 gggttccaag ttttggtata gggaaggatt ttattgatag aactttgaaa tacgattcaa
    37981 ctagtagctt gggaatatat acagaagaag gaaagtatca agaaatcaaa gatagtatca
    38041 agaagattgc taaaaataat ggatttttag agactgattt tatagatagt agaattgagt
    38101 caaataaggc tactatttca tttataaaga taatgggata cacgctaaca ggcataatag
    38161 gtgtgattgg atttatgaat cttgtcaaca caatgataac tagcatagtt actagaaaaa
    38221 aagaacttgg tatgcttcaa gctattggtc ttactaataa acaattagtg aaaatgctta
    38281 attcagaggc aatgtattac acttctggaa tgatgatagg aagtattcta tttggaggta
    38341 tattagggta tattgctgtt atatttctaa aaaagactgg attatcttat gcaacatatt
    38401 ctttgccaat agtaccaatc ttgttgatga ttgtgtgtat tcttatagct caatttataa
    38461 caacttattt gataggacga agttttaata aagaatctct tattgataga gtaagataca
    38521 gtgaataaaa tacaaatatt tatggttagt ttaactagca aaaaacatat aaaaaaatat
    38581 agttaaagag attcataatt aaataggtta aagcatagta cagagcaaac aatttaagca
    38641 gatgtttgct ctgtatttta ttatgtaatc aataatatat ctaatatact gatattattc
    38701 atgttagaaa atgtaccggt acaattctaa aaaagtataa aattgtacca tataaaatga
    38761 aaattttgta taataatcgt atagaaaata aataggagct gatatatatg aataagaaga
    38821 ttaattatgc accaggacca actgaaacta gggaaaatgt aagattagca agagcagaaa
    38881 aaacaacaaa tccagatata gatattgatt ttgttgaatt ttataaaaag acatgcgaga
    38941 agtttggtaa tataattgga acaaaaaacg atgtttacat attgagtggt gaaggaatat
    39001 taggtcttga agcagcatgt gcatcactta cagaaaaagg agatagagta cttgtaattg
    39061 acaatggtat atatggagaa ggttttaaag attttgtaac aatgtatggt ggggagtatg
    39121 tgcttttttc aagtgaatat actaaaagta tagatattga tgaattgaaa gcatttttag
    39181 atgaggatag taattttaaa tatgcaacag tagtgcattg tgatactcca actggcgtat
    39241 taaatgatgt aagcaaaata tgtccattac ttaaagaata tggaatatta actgtagttg
    39301 attctgtagc aggtatggta ggagaaagat taagtgtaga tgaatctaaa atagatataa
    39361 tattaggtgg ttcacaaaaa gctatttctg caccagcagg acttactata gtcggaataa
    39421 gtcaagatgc aaaaaattgt ataaaaaata gaaaaactga tgtaataggt ttttattgta
    39481 atttaaatat atgggaaggc tattatgaaa aaaaatactt cccttataca atgccaataa
    39541 gtgacataat ggggcttgat aaagcacttg ataatatatt agaagaagga atagaaaatg
    39601 tattaaatag acatgaaaag atagcttata gtgttagaaa atctattgag gaatatggtc
    39661 tagaactatt tttagaagaa ggatattcta atacagtcac agctataaaa attcctgaaa
    39721 gtataggagc tttagaactt actgattata tgcttaaaaa ttataataca cttgttgcaa
    39781 catcactaaa tcaatatatg gatactattt taagaatagg tcatatggga gagaatgcaa
    39841 atctaaataa aatagaacat gtactaaatg ttttggataa aagtttaagt gcattaggat
    39901 tcaaaggaaa tggaaatttg ttaaatttat ttaataagta ttatttttaa tagatagggg
    39961 gatatagtat ggaagttaca gctaagcaaa aatttacaac gaaagacatg gtggaaacct
    40021 cactattaat agcattagta tttattgcaa ctaaatttat aaacataaga ttaccaatat
    40081 ctataaatgg tgggcttgtg catttaggaa ctgcaatgtt gttcatatca gcaatagtat
    40141 ttggaagtaa aaaaggtgct ttatcaggag ctattggtat gtcacttttt gatttgatat
    40201 ctgggtggac tttatgggca ccgtttacct ttatagttag aggagttatg ggttatttac
    40261 ttggaaagat tgcatgggca aatggtaata atgggaataa ttttttaatc aatgtaatag
    40321 gaatatgtgt ttcatcaatt tggatgctat ttggatatta tgtaacagaa gttatacttt
    40381 atggtaattt tatagtacct cttacttcaa taccaggtaa tttaatgcaa gtattgatag
    40441 gtcttataat agcgcttcct atttctaagg tattgaaaaa atgtataaaa taaatataaa
    40501 gtgaactaaa atttatgata gcataaatta tactgtataa tattaggatg gagtacataa
    40561 ttaaaaaatt ttagtataga ggttagttca caatgttatc agttacaaga tattcaatca
    40621 aaaatgttcg tttaaagccg tgggtaaagt ttatttggta ttttgagaca aaatcaaata
    40681 tactgttaaa taataagtta cttccgacag acagtataga tattattctc aatctttctg
    40741 atgttatgga atataaaatt gaaaatcaag actatattgc tagtaatatt cattttaatg
    40801 gaataaggga taaacatgga tttattattc aacatggaaa tatacgtgtt atagggattt
    40861 ctttttatcc atttggtctc tatccatttt taaagatacc aatttcagag tttaacagac
    40921 aaattgttga cctagaagca gtatcaaaac tatttgcaaa aaaattagaa gagtcattaa
    40981 atccaacaaa agcaatagaa aaagttgttt tatgtttaga agaagtatta ttatccatat
    41041 tagaagaaga tttaatttca aataaatatg taaagcttct aaattcattt atatatgtta
    41101 atagatatag caatataaaa gcattttgtg atgatacaaa tataaatatt aaaacattgg
    41161 aaagaatctg tttaaaatat acagggtata cacctaagat tttaaagaga atttacagat
    41221 ttaaaatggc aagcaatcaa cttatttata attataaaga tgatgaactt tttgatttta
    41281 tttatgaaaa tgagtactat gaccaagcac actttataaa agaatttaaa aaattttcag
    41341 ggacgtcacc aattaaattt agtggtgaga ataagacgat taaagaaaat ataaagtaca
    41401 gctatttata attatactgt cgatttttta caatacctcc aatttatttt ttgatatgat
    41461 ataggaaaat aaattggagg ttttacatat gaaaattatt acagaaatag ttgagtttaa
    41521 tgtggaagaa aatttatcaa aagaagagtt tatagaaatt gtaaatgact tagaattaaa
    41581 atttcattct attcaatctg gttttattga tactgagctt ttatatgatg aaaagtctca
    41641 aaagtggatt atgatacaac attgggaatc tatagaagac ctcaagagtg tttctagtaa
    41701 aatgtttaaa gattcttcaa ctgagaaatt tagaaaagct ttaaagaatc aaactgttaa
    41761 gatgaatata ataccacaaa ttaaaagttg gaaattatag aactgaatgt aaaagatagt
    41821 taaatagtat caaactaaat tagccaaata acattttgta tttaaattgt tatttggctt
    41881 ttagtgttta ttttaatatg agtgttatat actagaaaaa ccaacaaact gttagagtgt
    41941 ggattatcat aatgtcatat tcgggagcta aaaggcttaa tactgatttg ccaattggtc
    42001 aatccatgca tgtccatctg gatatgctgt tattaaagca tttacaagcc aatccctttg
    42061 ctcttgtgat aatagtggga ctgttacatc gaagtctttt tgataaccaa gcctatctat
    42121 gtatattgat ttgcaaaaca aaaccacttc gggagccata tatgggatat tatctgatgt
    42181 tatcagtctg gccttatcca tatctcttat aattctttga ttttgggaaa aaagaaattt
    42241 tccatctttc tgtggattat atatcaattc aataaaatcg aactgtaact gttcgtcaca
    42301 ctgtatattc attttatagt tatcatcctc gacttttttt aattcaataa atgagcaatc
    42361 aggcttaatc gcaaaaacac aaatttgctc tatatgttgc ttgttaaggt cgataatagg
    42421 tcgtaataaa taattaccca agggctcata tatatcccat ccttgattaa gcatatactg
    42481 aatgctcttt tctttatctg tgtcagaaat ccatatgtca atatcactgt gtgggcgaat
    42541 atgcatattc gcaaataatt ctagggcata tccaccacat attgaatatg aaaagccaca
    42601 atcattaaaa aagtttacac aatctttaat tagtttgttc atagcaacct ccttttgcat
    42661 gtactgaaat aaattaaatt ttttaaaata ccaactaaat gtttgttttg tgtaaatata
    42721 aaactgccga tattaaggca gttttatatt tactatttag aaaatttatt aacaaatttt
    42781 tgatattcac acattatctt atcattcata aaagaaatac ccataaaagc agcttgctca
    42841 ccaaacaaag taaaacgttc tatcatctgt tgtggattac ctggataaac tgttggaata
    42901 caccatgtgt ttagaagaaa accaaacatt tgagcagcaa tctctggatt tttaacttcc
    42961 atagaattat cttgatttcc ttcttcaatc aatgctttta tccaaggcac aatatcattt
    43021 ataatctcat caaactgcgt agctaaatac ttaggattac gcaatagtga tgatgacatt
    43081 aaaaaaatgt cttgtttatt gtgctgttca taagtatgaa gcaacctata ttgtaatttt
    43141 tctaatgcgg tctttccttc atcatttttt acctcatacc cataaaatcc atcattatac
    43201 aaccgacttg tgattgcctt aattatttcg tcttttgatt taaagtgatg ataaatagca
    43261 cctcttgaaa ggccatctaa attatctaca atgtcttgaa tcgttgtctg ctcataccct
    43321 ttttctaaga ataacttatg tgaaatatca agtatttttt gtatagtatt atcaggttct
    43381 ttttttcttc ccaaaaaact cactcctcga aacaatcatt tggtatgttt atcttagcaa
    43441 atacataatg atttgtcaat gtgtcttttt agcatgttga acaggtatat tgccatgctt
    43501 tcattatttt atttttttgc cttatactgg actatatttg cagtttccta atatatttgg
    43561 ctcatattta aataccatta tctaaaagca ctattgctgt acgagaggca ataggcattt
    43621 tagaagaagg aggagttgag ctaaagtata catcataatc tcttaagttt tctttttttg
    43681 tgtttcctcc aaactctacc atatcagtat ttaatatcgt tttgtagttt gtattttgaa
    43741 actttgaaat atcaactgat tgtattagat tagggtggaa attaaatata aataaatatt
    43801 tgcccttact gtacaccaaa atctttttat cattatccat atgtataagt tcatgactat
    43861 tttggataaa aatatcactg tattttgtaa gttcgagcat agatttatca aaatttaata
    43921 attgtttata ctttaagttg ttatcttctg aaaggctcca ttgtcttctg gcatacttat
    43981 agctccaatt gttgccttct cgtggaaaat caatccactc aggatgtcca aattcatttc
    44041 ccataaaatt tagatatcct tcacctgcta gagaaaaagt tattaattta attaatttaa
    44101 taagagatat tgctctattg ataacatggt tatttgagtt tatttccata ttccagtaca
    44161 tttccttgtc agctagccaa aaaataattg ttttatcgcc aactaatgct tggtcatggg
    44221 attcacaata tcctatattt ttttctccag gtcgtctagt agtaagttca taccacattt
    44281 tgcccaaatc ccagtcttca tcagataagt tagagatagt ttttatccaa aaatcaggta
    44341 ctcccattgc gagtctatag tcaaatccaa ttccgccgtc ttttataggg atacacatac
    44401 caggcattcc actcatatct tctgcaatac ttatcgaatt aggtttaatt tctttcatta
    44461 attcatttgc aaattggaga tatgttatag cttcaatatc tgtattcata ctaaaatatt
    44521 tttcataact atcaaatgaa actccaagac catggttatg atataacatt gaagttacac
    44581 catcaaacct aaagccatca aaatggtact catttaacca aaattttata tttgaaagca
    44641 aaaaatgtat tacttcaggt ttaccatagt taaaaagttt tgtaccccaa gcaggatgat
    44701 ttccctttga acctgagtgg aaaaattgat gttcacttcc atcaaactca tttattcctt
    44761 ctaaagtatt ctttacagca tgagaatgaa ctaaatctaa taaaacagca atccccattg
    44821 aatgagcagt gtttatgagg ttttttaagt cttcaggagt accaaaccta gatgagattg
    44881 cataaaagtt tgaaacttgg tatccaaaag atgcataata tggatgttcc ataatagcca
    44941 taagctgaat ggtattgtat ccagcttttt ttatcttagg aagtattttt tcagtaaatt
    45001 cattataagt tccaatagat tcgctttcag tagacatacc tatatgacat tcatagatta
    45061 gtggagaagt tatgtttttt aagtcaaaat cattgtcagt ccagataaat ggagtttttg
    45121 gttgccatat ttgtccatta aatccaccaa aatctttttg aattactctt tttatatata
    45181 atggtatacg gtcaaatgtt tttccatttg cagtaacttg tacctttacc tctgatttgt
    45241 gaggtaagct attttgacca ggaataaata tttcccaatt accagaccca atttttctta
    45301 gaggatgaga ttttctattc caattattaa agtcaccaat tagtgacaaa ctatctgcat
    45361 ttggagccca ttccctatag aagaatccat catttgtctg atgaaatcca tagtaaagat
    45421 gagcatttgc aaaagaacta aaatctttat agtttttcaa tgtttttgat ttcatatcct
    45481 cataatgttt cattcgcttt tttatatcag attcaaatgg ttgtaggtat acatcaatat
    45541 ctaaaatttt aaatcttttt tgacttttca ttgtatttct cctccataat aaatattaca
    45601 tctaaaaaat aatatttatc tcatactatc ttctctgtat aattgatata tattatataa
    45661 tcctaataaa aagcttaagc caccaatgat taatttcata atttacctcc ctagtattta
    45721 tacttatata tcgtattcat caaaatttaa aagtttgttt aaaactcttt taataatatc
    45781 ataatcaaag cctttatagg aaagatgttg agaaattttt tgatatattt tctttttatc
    45841 ttctttcttt actctttcat accttttttt tgctagatac atagcatttt caaattcagt
    45901 gtccttgtct aattcagata ctgcttcatt gattgtactt ctattgatac ctttattata
    45961 taaattctgc ttaattctgt ttttgccaca tttatttagg ttaacatttg tgttaacaat
    46021 tttttgagct aatacactgt catttacgag attataactt ttaagaaaat ctatgaccct
    46081 ctcaattata ttttcatcaa attctgatga tagtttttct tttaattttt tttcagattg
    46141 gtcagcatga gataaaatat ttaatgcttt atttttagct tttatataca tttcatcatc
    46201 taaaataggt tttaattctt cttcatttat ttcattccct ttttttaaat taaatgtata
    46261 aaccagttct ttaaatatag caataaaaaa tttatcatct acataaatat taactctatc
    46321 atcatttctt ttttgttgtt ctatttttgt aattataccc ataagtcctc cttttttata
    46381 tgtaattata ttaacaaaag caactaataa aatttaacaa aaaacttata tttgtatgtt
    46441 atattttatc aaaattaaga caaaatagta aaaagttatt tttattaaaa agtaaagcag
    46501 aattatgtta aataattatt ttttaaagtg ttttttagca tataaatata attatatacc
    46561 caaaaaaaca aattataagc tttaagaaat aaaaaataat ggtacaatag tattataaca
    46621 ttaaaaggag acaaaatatg agaagtgaaa atctagttaa gatggcagag ttgaaaaata
    46681 ctgaaaaact agagaaattt aatcgccata aaggcaaaat caaaataggt atattgggag
    46741 gtacttttga ccctatacac tatgctcatt tagcaacagc agaatttatt agagacaagt
    46801 atgacattga caaaataatc tttataccat caggaaatcc accacataaa ttatgtatca
    46861 ctacagataa gtatgataga tataatatga cacttctagc aactgaaagc aacgaagatt
    46921 ttttagtttc aaaagtagaa atcgaaagaa agaaaaggac ttatacaata gatacattaa
    46981 aatacttaaa gaaaaaatac aaaaatgcag acatttattt tataacaggt gcagatgcaa
    47041 tttgtagtgt tgaagagtgg aaagatgtaa agaaaaactt tgagttagct acttttatag
    47101 ccgcaactag accaggaatt agtttgttaa ggtcacaaga gactatagaa aaactaacta
    47161 agaagtataa tgctgacatt ataacagttt atgttccatc actggatata tcgtccacat
    47221 atataaggga acagctaaat gaaggcaagt caatacgtta cttagtaccc gaaaatgtag
    47281 aaaattattt atatgaaaat aaactatatc aatatggaga tgattaaatg aacttagaaa
    47341 atattaacca aagactaaat caaatgctac cagtggggag gcttagccat tcaaaaaatg
    47401 ttgcaaaatg tgctgaaaaa ttatgtgaga tttatggatg tgacaaagaa aaagcttact
    47461 tagcaggaat gattcatgat tgtgcaaagt atttaagtga caaggaaata gaggattatg
    47521 taaataagta tgaaatatat ttagacccaa tagaggatgg aaatcgttcc ttgtctcata
    47581 gtgtaattgg ggcgtacata tgcgagtatg aatttgaagt ggaagatgaa gatattataa
    47641 atgcaatcaa atatcataca acagggagag aggacatgtc tcttttagaa aaaataattt
    47701 acatagctga tttaattgaa gaaggaagaa aattcccagt tgtagataca ttaagagaat
    47761 tagcatatgg agggaagctt gatgaagcac ttttaacttc ttttaataat actttaatgt
    47821 ttgtcatcaa taaaaaagaa gagatacatc caagaacagt tatggcaaga aactatctaa
    47881 ttaaagaaaa gttattataa aaaagctata taaaggcaaa catattaata aatttaaatt
    47941 tgactatgtt acaaaatcgt attaatatgt tacaataact aagtgtattt taattaggaa
    48001 agtgaggaat ttttatatga cagtggaaca aatgacaaaa atagcatatg atgccataga
    48061 agataaactg ggtcaagata cagttataat aaatatagga aaagtttcta gtttgtgtga
    48121 ttattttatt attactacag catcttctca gagacaggta aaagctattg ctgacaatgt
    48181 ggaagatgaa ttagcaaaac taggattaga gccaagaggt aaagaaggac aaggaactca
    48241 aacttgggta ctccttgatt atggagacat aatggttcat gtatttaatg aggaaaatag
    48301 agggttttat aatttagaaa aattatggaa agatgcacct tatatagata ttgacacatt
    48361 agcataaaca tgataatata tgttattata attagaaaat ttcaaaaatt atattaaatt
    48421 gaatagacta gagc
//
LOCUS       DAELTA010000009        46386 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.009, whole genome shotgun sequence.
ACCESSION   DAELTA010000009 DAELTA010000000
VERSION     DAELTA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.009"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            419..3967
                     /locus_tag="KPG13_000439"
     CDS             419..3967
                     /locus_tag="KPG13_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0984186.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4152..6086
                     /locus_tag="KPG13_000440"
     CDS             4152..6086
                     /locus_tag="KPG13_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG0984187.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6192..7187
                     /locus_tag="KPG13_000441"
     CDS             6192..7187
                     /locus_tag="KPG13_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0984188.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7159..7944
                     /locus_tag="KPG13_000442"
     CDS             7159..7944
                     /locus_tag="KPG13_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0984189.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7932..8630
                     /locus_tag="KPG13_000443"
     CDS             7932..8630
                     /locus_tag="KPG13_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984190.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8753..9226
                     /gene="ybaK"
                     /locus_tag="KPG13_000444"
     CDS             8753..9226
                     /gene="ybaK"
                     /locus_tag="KPG13_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG0984191.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9423..10262
                     /locus_tag="KPG13_000445"
     CDS             9423..10262
                     /locus_tag="KPG13_000445"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG0984192.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10275..10823
                     /locus_tag="KPG13_000446"
     CDS             10275..10823
                     /locus_tag="KPG13_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG0984193.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10889..12070
                     /locus_tag="KPG13_000447"
     CDS             10889..12070
                     /locus_tag="KPG13_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG0984194.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12365..12751
                     /locus_tag="KPG13_000448"
     CDS             12365..12751
                     /locus_tag="KPG13_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0984195.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12901..13164
                     /locus_tag="KPG13_000449"
     CDS             12901..13164
                     /locus_tag="KPG13_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0984196.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13293..14135)
                     /locus_tag="KPG13_000450"
     CDS             complement(13293..14135)
                     /locus_tag="KPG13_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0984197.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14430..15164
                     /locus_tag="KPG13_000451"
     CDS             14430..15164
                     /locus_tag="KPG13_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984198.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15174..16307
                     /gene="nagA"
                     /locus_tag="KPG13_000452"
     CDS             15174..16307
                     /gene="nagA"
                     /locus_tag="KPG13_000452"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0984199.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16406..17155
                     /gene="nagB"
                     /locus_tag="KPG13_000453"
     CDS             16406..17155
                     /gene="nagB"
                     /locus_tag="KPG13_000453"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG0984200.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17330..17602
                     /locus_tag="KPG13_000454"
     CDS             17330..17602
                     /locus_tag="KPG13_000454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984201.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18262..18966
                     /locus_tag="KPG13_000455"
     CDS             18262..18966
                     /locus_tag="KPG13_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984202.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18968..20455
                     /locus_tag="KPG13_000456"
     CDS             18968..20455
                     /locus_tag="KPG13_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0984203.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20660..22273
                     /locus_tag="KPG13_000457"
     CDS             20660..22273
                     /locus_tag="KPG13_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0984204.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22636..23466)
                     /locus_tag="KPG13_000458"
     CDS             complement(22636..23466)
                     /locus_tag="KPG13_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0984205.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23539..25266
                     /locus_tag="KPG13_000459"
     CDS             23539..25266
                     /locus_tag="KPG13_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984206.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25303..27165
                     /locus_tag="KPG13_000460"
     CDS             25303..27165
                     /locus_tag="KPG13_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984207.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27498..28187
                     /locus_tag="KPG13_000461"
     CDS             27498..28187
                     /locus_tag="KPG13_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG0984208.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28292..28978
                     /locus_tag="KPG13_000462"
     CDS             28292..28978
                     /locus_tag="KPG13_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG0984209.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28995..30902
                     /locus_tag="KPG13_000463"
     CDS             28995..30902
                     /locus_tag="KPG13_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG0984210.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31355..32044
                     /locus_tag="KPG13_000464"
     CDS             31355..32044
                     /locus_tag="KPG13_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984211.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32480..33019
                     /locus_tag="KPG13_000465"
     CDS             32480..33019
                     /locus_tag="KPG13_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984212.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            33038..34081
                     /locus_tag="KPG13_000466"
     CDS             33038..34081
                     /locus_tag="KPG13_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984213.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            34083..34919
                     /locus_tag="KPG13_000467"
     CDS             34083..34919
                     /locus_tag="KPG13_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984214.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            35024..35698
                     /locus_tag="KPG13_000468"
     CDS             35024..35698
                     /locus_tag="KPG13_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984215.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35698..36750
                     /locus_tag="KPG13_000469"
     CDS             35698..36750
                     /locus_tag="KPG13_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0984216.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36980..37828
                     /locus_tag="KPG13_000470"
     CDS             36980..37828
                     /locus_tag="KPG13_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0984217.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37818..39482
                     /locus_tag="KPG13_000471"
     CDS             37818..39482
                     /locus_tag="KPG13_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0984218.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39531..40787
                     /locus_tag="KPG13_000472"
     CDS             39531..40787
                     /locus_tag="KPG13_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0984219.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40810..42891
                     /locus_tag="KPG13_000473"
     CDS             40810..42891
                     /locus_tag="KPG13_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG0984220.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            42928..44013
                     /locus_tag="KPG13_000474"
     CDS             42928..44013
                     /locus_tag="KPG13_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0984221.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            44067..45191
                     /gene="wecB"
                     /locus_tag="KPG13_000475"
     CDS             44067..45191
                     /gene="wecB"
                     /locus_tag="KPG13_000475"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG0984222.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            45269..46243
                     /locus_tag="KPG13_000476"
     CDS             45269..46243
                     /locus_tag="KPG13_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0984223.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        18512 a         4687 c         8028 g        15159 t
ORIGIN      
        1 attaatctaa agtatatgtt tttatatggt ttaaatttta tgtatacaaa attaatttgc
       61 taactgaatc atataaaaaa aaacaaaata ttttttagag ggaatgatta gatagaattt
      121 ttttgttatg aaattaattg aagagtaatt aattagaaaa ggaatgaata ataatgaata
      181 tattacatta ttactttttg ataaatcaaa aataaagtta taatttttaa tattaattaa
      241 gaatgatgaa atatgaataa agattttaca aataaatctt aagctatctt atttaattct
      301 aatacaggaa gaaggtaaat gtatattgaa aaaatcatag aaatagaagt aaacaattta
      361 aataaataca aaggtttgca aaagaagtaa tataattata tacaatgagg atgtgagtat
      421 gatgaacaga ctaaagccaa gaattttagg tattagtatc ttgtttatta ttataattat
      481 tttaacatgt ggagctgttt tcaagtttaa atttaaatta gataataaat taaaagaaac
      541 cacacataca tatttgtctg agtttacaga acacaatata attaatctca agacaagact
      601 tagtggtcaa tttgacatgc ttgaatctat tgctaacttc ataggaaact tgaataatat
      661 aaatgatgaa atggtgattg atttaatgga ttctgatgta aagcgaagtt ctttaattag
      721 aatgactatc tctacattag atggggaatg ttattctaat gatggaatta cccataatgt
      781 tcaagataga gattatttta aaaaagctaa aaatggagaa aagaatatat cagagccttt
      841 aaaatctatt gttaataata aagaaataat agttttatct accccagttt ataaaagtgg
      901 gaaaatagtt ggtgttttaa gtggaattta tgattcaaaa aagttaaatg agttattagg
      961 attatctgca tttgatgaaa aagcagatgt ttttgttgca aagaaaaatg gagatatagt
     1021 tgctcaaaaa acaaataaaa atagaaagac gctatcatgt atatttcagc tttttgacaa
     1081 agataattgt gatgaagatg attgtctaaa aactataaaa gaaaatatga aaaatgataa
     1141 gtctggatat tttaattata acttagataa tgaaaaattt atgttaaact atcagccact
     1201 tggagttaat gattggtatg ttttttctgt tgtgccaaat aaaatagtat ctattcagtc
     1261 agagaatata tcttatgatg tatatatttt tgcattagaa atactactag ttatattgat
     1321 ttttataata tatatagcat gtattataaa taataacacc aaaattatta taaatgaaaa
     1381 agaaaaattt aaagtactca ctgataatat attaggaggg gttaaaacct gtttaaaaga
     1441 tgaatatatg actatgacat atgtaagtca ggggttttta gagcttacag gttactcttg
     1501 tgaagattta aataaaatat ttaaaaatag atttaaagac atgatttata aagatgattt
     1561 agacaagttt aattgtatta ttctaaatca attagaaaaa caagataaaa ttgaagttga
     1621 atatagaatt gtaagaaaag atggaagtat aatttggatt ttggagagaa gtaagtctat
     1681 aaatgataat aaaggaagtg agtgtattca gggagtttta actgatataa cagaacttaa
     1741 gaaaatacaa gaagaattag attcaaaaag atttgaaata caggctatca atgatagtat
     1801 tgttggtgga atagtgataa ctgagataaa tgatgatttt gatagtatat atttaaatga
     1861 aggttattta aatatgattg gatatacacg caaacaactg gaagatgagc taaataataa
     1921 attaatcaaa cttattttta aagatgatag aacagaatta attaatagta taaacaaaca
     1981 actcaaatta agtgatacat ttgcatctga acaaagagtt gaaaaaaggg atggcactat
     2041 tatatggatt ttattaaaag gaagaaagat gatttatgat aatggaaaat ctgtactttg
     2101 ttgtgttatt gtagatatta cttctgctaa attattagag caagaactta aaaagagtga
     2161 agaacgtcat gaaatcattc taaagcagac gcaaaatatt ataatagaat gggatataga
     2221 aaaagataaa atgaatatat cacctaattg gtataataag tttaaatatg accctataac
     2281 tgaaaacgca acaaaaaata taattaaatc agtagatata tatcatgatg atatggataa
     2341 gtttatttca ttaattaaac ttacttcaca aggtagtgag tatgaagagt gtgaaattag
     2401 acttagaact agagatacta tttacgcttg gtttagtata aaggcaacaa ctttatttga
     2461 taataatggt aaaccttata aagtagtagg aagtttgact gatattgatg aatcaaaacg
     2521 agaagctgaa aagttgaaag aaagggcaga aaaagaccct cttacagatc tgtataataa
     2581 aaaagcatca caatcactta tagaggagta cataacattg gataaaaaaa gaaaaggtgc
     2641 tttaatgatt attgatattg atgactttaa aggaataaat gataatttag gacatttata
     2701 tggggatgct gtattaagtg aaattgcaaa tgacctaata aatctattta gagcaaccga
     2761 tatagtaggc agaattggtg gagatgagtt tattgtattt ataaaagata tttctgaaac
     2821 aagagatatt ataaataaag cagaagaatt ggtaaaaaca tttgaaaggt cttttttagg
     2881 taaaggacga aattataaaa tatcattaag tgtaggaatt gctaagtttc cacaagatgg
     2941 tatggactat atgacactat ttagaaatgc ggatatagct ctttactcag ccaaagggaa
     3001 gggcaagaat tgttatacat tgtatgataa aaaatttgat aaagtccaat atataactaa
     3061 tacaagaaat gcaggagata gtgataatgt agagatgtct tttaaagaac atatcacaaa
     3121 ttatatattt gatatactat atgaatcaag tgatatagaa gtggcagtaa atttaattct
     3181 aagtattgta ggtaaacact ttaatgtgag tcgcatgtat atatttgaaa attcagatga
     3241 taatcgagta tgtaaaaata catttgaatg gtgtaacgaa ggtgtaacct cggaaattaa
     3301 taatcttaaa aatatttgtt acaaggattt aggcaactat cttttaaatt ttaatgcaga
     3361 tgggttattt tattgtaatg atattgaaaa tttaggtagt gatgtgaaaa aagtacttaa
     3421 gccacaaggc atacgttcaa tattacaatg taaaatttta gagggtaatg aatttagagg
     3481 gttcatagga tttgatgaat gttcatataa tcgtatgtgg actaatgatg aagtgaacac
     3541 tttgacattt atatctaaaa cgctcagtat gtttttatta aaaatgcgta ttcagagtaa
     3601 gctagaagag tctttaacaa ttcaaaaatc tgtgatggat aatatggata tatgggcata
     3661 tgttgttgac aggaatacct atgaattact atttatcaat aaaaaaatgc ttgagtcagc
     3721 accaaacact catgtaggtg atttatgtta taaagcgata tggaataatc aagataaacc
     3781 ttgtaaaaaa tgccctatga tatatttaaa agatggtgat gatacttata aatcattcat
     3841 gcataataaa aagtttgagg tacaggcaaa tataactaca actactttga aatggtttga
     3901 tggtaaagat gcatgtttaa tatttggaag tgaaataact agtattgaag ctgaagaaaa
     3961 tacttaaaat actgtgttat atatatactt aaaatcgcaa tttgaattta ttagacttaa
     4021 aatgtctata taaatcattt aagttgtggt tttaagtatt ttttatgata taattaagcg
     4081 ttaagaacaa aattaaaaga tacttttgta taaaaaaata tttgttaaat atatattgag
     4141 caggtgataa tatggaaaat aagtttttac caataagcaa acaagatatg atagatagag
     4201 gatgggaaga attagatttt gttctagtta caggagatgc ttatgtggac catcatagtt
     4261 ttggaacagc aataatatct agagttttag aaagtgctgg gtataaagtt ggaataatag
     4321 ctcaaccaga ctggagaact acagatgatt ttatgaagct aggaaaacct agacttgcat
     4381 ttctagttaa tgcaggaaat atggattcaa tggtcaatca ttattctgta agtaaaaagc
     4441 atagagaaaa ggatatgtat tctccaggtg ggaaaatggg ttatagacca gatagagcta
     4501 ctatagtata ttgcaataaa ataagagaag catacagtga tgttgcaata gtaataggtg
     4561 gaattgaagc gagcttaaga agatttgcac actatgatta ttggagtgat aaggttagaa
     4621 agtctatgtt aatagatagt ggagcagatt tattagttta tggtatgagt gaaaagcaga
     4681 ttgtagaagt agccaatgca cttaatgatg gatatgaccc aaaatacatt agacatattg
     4741 atgggacatg ctatatttca gatacattgg aagaaatata tgataaatat atattaattc
     4801 catcatataa agaaatttgt gaagataaaa tgaagtatgt agaagctttt aaaattcaat
     4861 atgatgagca agacccattt agaggtaata taattgttca acctcatgga agtaagtatc
     4921 tggttcaaaa taagccagaa aaaccactta gtagagaaga attagatgaa gtatatggat
     4981 taccatatca gaagacatac catcctgtat atgaaaaatt tggaggaata ccagctatag
     5041 aagaagtaaa atttagtata gtaagttcaa gaggctgttt tggtagttgt tcattttgtg
     5101 caataacatt tcatcaaggt agagcagtgc aaagtagaag tgaaaaatca attattgatg
     5161 aagcaatagg aataacaaat ttagatgatt ttaaagggta tatacatgat gttggaggtc
     5221 caacagctaa ttttagaaga ccagcatgta aaaaacaaat tactaaaggt gcttgtaaaa
     5281 atagacaatg cttatctcca tcaccatgta aaaatttaga tgcagaccac agtgaatact
     5341 tacatttact tagagctgta agaaaattac ctaaaataaa aaaggtgttt gtgcgttctg
     5401 gaattagata cgattatgta atggcagata agaacaataa atttcttaga gagttgatag
     5461 agcatcatgt aagtggtcag ttaaaggtag ccccagaaca tatatcagaa gaagttttaa
     5521 agtatatgca aaaaccagca ggaaaaactt atgataaatt tagacagaag ttttttgcta
     5581 taaatgaaga attaggtaaa aagcaatatt tgatacccta tttaatgtct agtcaccctg
     5641 gttctacctt aaatagcgct atagagttag cagagtattt aagagatact cactaccaac
     5701 cagagcaggt tcaggatttt tatccaactc cagggactct atctacaacc atgttttata
     5761 caggtattga cccgcttaca atgaagccag tttatgttcc taaatcaaaa cgtgacaagg
     5821 caatgcaaag ggctttatta caatatagag cacctagaaa ttatgatttg gtatactctg
     5881 cgttagttga agcaggaaga gaagacttaa ttggttttgg acatagatgt cttataaagc
     5941 caaaaaatga aaagccttat tttaatagaa ataattctaa aaaaaatgta tctaaaggaa
     6001 caaataaaaa caaaaaaact aatacaaata acagaagtaa taaaaatcaa caaaaaagtt
     6061 caactaaaaa aaagaaaaaa cattaaagta taaataaaaa tacttaaaaa agaaggatta
     6121 ttcgataaat taacagaaat tagtataaat aaatataatt attttaatga cgttaccttt
     6181 gggaggaaga gatgaaaaag tatatttcta taatattatt agtggtgttg actatggttg
     6241 ttttggtagg atgttcacca ggaaaagata accctaaaaa taaggagcta tcagttgtca
     6301 aacaagtaaa agtagctgtt ccagatggac taccagcagt ggcaatagct aaacttgcaa
     6361 atgaaaatcc agagattaaa gaaggatatg aaacagtgta tagcattgaa aagacacctg
     6421 aagcgatttc cactagagtg atgaaaaaag atgcagatat agctatagtt ccttcaaaca
     6481 tggctgcaat agcgtataat aaaacttcat catataatat tgttggaact gtaggaatgg
     6541 gttcacttta tttggtgtca acagaaaata taaaagatta ctctgattta gttggtaaag
     6601 aagttggatg tactggaaaa ggacttactc cagacataac tataaaaagt cttttgttac
     6661 aaaaggatat aaattattca gatataaaat ttaattatgt aaattcagca agtgaactgg
     6721 taccattatt ggctactggt aaagttaaaa caggtattgt tccagaacct gcacttagtg
     6781 ctttgatgtc taaaaatcca gacataaaaa ttataaaaag tttaaatgac tcatggaagg
     6841 aagtaagtgg ttcaaaagat ggttatcctc aatctacttt aattgtaaag tctgaatttt
     6901 taagagataa taaagatttt gttgattctt ttgtaggtca attatcaaat agtatagatt
     6961 gggcaaacaa aaatcctgaa gaattaggtg catattctga aaaaataaaa atatctactg
     7021 aatcaaagat aataggaaaa tctcttgaaa gagctaattt gaaatatatt ccagttaagg
     7081 acatgattaa agattataaa aattactatg agaaattagc taattttgat gataagacat
     7141 taggaggcaa agtaccagat gaagcaatat actttgttga gaaataaaaa aaataaagaa
     7201 caatttatag ataaaatgga ggtaacactt tcttgtgtta tcctcttatt tttatggcaa
     7261 attattgctt taaaaattga taatgatatt tttttaccaa cagtaggtca agtgtttaat
     7321 agtataaaag agattatgct aaattctagt ttctatatag atatacttta ctctatgggg
     7381 agatgttttt ttagtttttt actggctatg atttttgcta ttatactttc aatagtgtct
     7441 tatttaaata gattttttag aaacttatta aagccaatca atgctttgac acgttcaata
     7501 ccaactatga tattggtagt acttgcactt atttggtttg aaaaggatag tacccctttt
     7561 atagttggat ttactatagt ttttcctata ttgtatgata acgttttggg cgctatattg
     7621 aatatagaca agaacttatt agaaatggct aatgtgtata aaataaggtt tatagataag
     7681 gtgttaaaaa tttatttacc agccataaaa tttcaaatta tatctatact agtatcaaca
     7741 ttttctttaa gtttgaaagt tgctatagca ggagaggtgt atagtcaacc tacatatggt
     7801 attggaacta tgatacagac tgaaaaaatt aactttaaca catctggaat atttgcatgg
     7861 ataattattg ttcttttaat atctgctatt ttacaaatag ctcaaaagtt tttagcaagg
     7921 agagcttttt tatggaaaag ataaagttga gtattgaaaa tataaataaa agatatgata
     7981 gtcgtattat atttagagat tttaatattg atttttatgt aaatgaggta aattgtatcc
     8041 ttggaaaatc tggttgtgga aaaactacac tcttgaacat aataagtggg attattaaaa
     8101 atgacaccaa caatctaaat atcaaagaaa atttaaatag agtaggaaat aagcttgaag
     8161 ctagttacat atttcaagat gatagactta tagactggtt aacagtagaa gaaaatataa
     8221 agatagttgt aaataaatac tataataaaa ctcaattaaa taaaatttgt gatgaatact
     8281 tggaattggt tggaattagt gattataaaa aattctatcc tcaaatgctt agtggaggta
     8341 ttagacaaag agtaaatatt gctagagctt ttatatatcc atcaaaaaac attataatgg
     8401 atgaaccttt taaatctatt gatgcaaaga atacacagct tataatggat aattttagaa
     8461 atatattaag aaaagaaaag agaactgtgt tatttgtaac tcataatatt gaggaagcat
     8521 tgtttttagc agataggata tttattttag gagactctcc aattagaata aaaaaaatat
     8581 taaaaaattc taaggaattg gaaaaaaatg aagttttgaa gttgatatag agttataata
     8641 atactgtcgt tttaaaatat atttggttaa aatctataag actatattaa aatagtacag
     8701 aatattcaaa actgtgtata atgttcgtat tttataaaaa ggaagtgaat gtatggttaa
     8761 gactaatgct atgagaatat tagattcaaa taagattgat tataaagtaa tgtcttatga
     8821 agtgaaaagt gaacatgtgg atggtgttga ggtggcacat gatataggaa gagatgtaaa
     8881 tgaagtgtat aagacattag taactcaagg agttagtaag aatatatatg tatatgtaat
     8941 accagtacat gaaaacttag acttaaaaaa ggctgcaaaa gtagctaaag agaaaagtgt
     9001 agagatgatt catgtaaaag atataaataa gcttacaggt tatattagag gcggatgttc
     9061 tcctataggt atgaaaaagt tttataaaac ttttgtaaat gaaagtgcaa agaatttaga
     9121 tacaattatt gtgagtgcag gaaaaatagg atatcaaata gaactatctc catttgattt
     9181 acaaagactt ataaaagtag aatttgtaga tgtaataaaa aaataagaat tacatataaa
     9241 atatagagct tatattctca ataataggtg ttgtaatatt aataaaatga ataagaattt
     9301 taaaataaat attcagttac ttatgtgtaa ttggtataaa aatattgaga aattttaaaa
     9361 acaatggtaa agtgtaagta taatcacgaa tattttttat aaagtaatgg agggatgttc
     9421 gcttgagaaa aataaaatct gaacaaatcg tcgagcaggt aaaaaaactt tgtatagaag
     9481 cgagcctata tttaggagaa gatgttttaa gttgtataaa agaaaaagcg aaaagcgaaa
     9541 agagtgaagt tggaaaaaat atattgaata tattagttga aaatgctgaa atagctaaag
     9601 aaaaaaatat accaatatgc caagatactg gtatggctgt attttttgtt gaaataggac
     9661 aagaagtttt aattgaagga gacacactta ctgatgctat aaatgaggga gtaagacaag
     9721 ggtatgaaga aggctactta agaaaatctg ttgtaagtcc aattaacaga gtaaatacaa
     9781 aagataatac acctgctgtt attcattatg atatggtaaa aggtgataaa attaaaatag
     9841 aatttgcagc taagggattt ggaagcgaaa atatgagtaa gatgaaaatg ttaaagccat
     9901 cagatggatt ggaaggaatt aaaaaattca taatagatac agtttcagaa gctggaccta
     9961 acccatgccc tcctatggta attggagttg gtataggtgg gactgtggac aaatgtgctc
    10021 aaatagctaa aaaggcactt tttagagaac taggtgaatt taataaggac gaaaatatag
    10081 caaaacttga aagtgaacta ttaacagcta ttaataaact tggtatagga cctcaaggct
    10141 taggtggtac tacaacagct ttgggattaa acatagagac ttttcctaca cacatagcag
    10201 gtctacctgt tgttgtaaat ataaactgcc atgcatcaag acataagaat gtagtaattt
    10261 aggaggaaaa taaaatgata aaaataacta cacctgtaaa tgaaattgat atagcaaaat
    10321 taaattgtgg agatacaatt agtttgagtg gtatacttta tacagcaaga gatgctgctc
    10381 ataaaagatt gatagattgt ataaataaag gtgaagaatt accatttgac gtatatggtc
    10441 aaggaatata ttatgtagga cctactccta caaagccagg agaagttata ggtgctgcag
    10501 gtccaacgac gagttataga atggatgatt tgactatacc tcttttagaa agaggtctta
    10561 gattgatgat aggcaaggga aaaagaagtg atgaagttat agaaggtatg caaaaatacg
    10621 gagcagttta tcttgctgca ataggaggag ctggagcgta tatttcaaat tcaataaaat
    10681 catgtgaaat tattgcatat gaagatttag gagctgaagc tataagaaag attgtagtag
    10741 aagaccttaa gcttacagta gcaatagata gctatggaaa taatatatat gagcaaggtc
    10801 gagcaattta tgaatgtaaa taatgtttag aaataaatat gaatatcaaa attaaaaata
    10861 gttgtgtata ttttaaggag gaaaattgat gtctaaggat tatgcaaaat tggcattaga
    10921 aatgcatgaa atcaacaaag ggaaagtaag tgtagaaagc aaggtagaaa ttaaaactaa
    10981 ggatgattta tcaacagcat atacaccagg ggttgctgaa ccttgtttaa aaatacatga
    11041 aaatcaagat gatgtttata gatacacttc taaagggaat ctagtagcag tagttagtga
    11101 tggaagtgct gtacttggat taggtaacat tggtgctgaa gcatctatac ctgttatgga
    11161 aggtaaagct atattattta aacaatttgc agatgtagac gcttttccaa tatgtttaaa
    11221 gactaatgat gtagatgaga tagttaaatc tgtagagttg atggagcctg tatttggagg
    11281 tataaatttg gaagacatat cttctccaag atgttttgag atagaagaga gattaaaaaa
    11341 atctttaagt atacctgttt ttcatgatga ccaacatggg acagcaataa tagttgcagc
    11401 cgccataata aactcaataa agttaattga aaataaaaaa atagaagatt tagaaatagt
    11461 tataaacgga gcaggagctg ctggtatagc catagcaaag atactactta atatgaatgt
    11521 taaaaatata atcttatgtg atagaagtgg agcattggaa gctagtatag aaaatctaaa
    11581 ctatgttcaa aaagaaatgt taaaagtaac aaatataaga aatgaaaaag gacctttaaa
    11641 agatattata aaaggaaaag atgtatttat tggtgtttca ggtccaggag ctgttacaaa
    11701 ggaaatggtt gaaagtatgt ctgagaagcc aataatattg gcaatggcaa atcctactcc
    11761 agagataatg ccagaagaag caaaattagg tggagcattt ataatgggaa caggtcgttc
    11821 agacttccct aatcaagtca ataatgtact ggcttttcct ggaatattta gaggtgcact
    11881 tgatgttaag gctaaagaga taaatgaaga aatgaagata gctgctgcat atgcaattgc
    11941 taatacaata agtgatgaag aaatatgccc agagtatata ttaccagatg tatttaataa
    12001 gaatgtagta aaaaatgttg ctaaggcagt taaagaagca gcgataaaaa ctaaagtaaa
    12061 tagaatataa atttttatat gttcatatat ccttatatta tcgggtatat gggcattttt
    12121 attataggtt tatactcata tatccttact tttatataca tatctttatg attaaaggtg
    12181 ttagaaaatg tagtaaattt tatactatca gactctatct ttattacttt agtagataca
    12241 tggttgtaaa ggtttataga aaaaaatata ggaaggcaga tttttattgt tgcgtatata
    12301 tgagaatgat ataatatgaa tattataaat taataaattt aaatttatgg aggtacaaga
    12361 gatgatggaa tataaagtta atgaccaaga acttaatgct tcaatgttta tcccattcgt
    12421 caacaggata tggccaggag attatgatat ggataaaaca cagactgcat tatcaaaaac
    12481 tttgaatata actgcttatg atgataaagt gcttgtagga tgtctgcgag ttctttcaga
    12541 tggttattat tttggaacaa ttacagaatt gcttgttctt ccagagtatc agaaacaagg
    12601 cattggtagt aagcttttcc agcttgctaa agagaacacc ccaactatgc tatattttgg
    12661 tgcacagcca ggagtagaag cgttctatga aaaaaatggg tgccaaagaa gtctgcaatc
    12721 ctacataata aaaaagaaag atagttatta aagttccagc tttcaataat cttaaaagta
    12781 caatatttta gtggaatcat ttcatttttt attactaata tgacataaac atgtcatatt
    12841 agtattgata tacttttaat atggttaaga gccaaagaaa aatataagga gtgatttaaa
    12901 atggaagaaa ctaaattttg ccaatgctgt gcaatgccaa tgggacaaac tgatgaatta
    12961 tatgggacaa ataaagatgg aagtaaaagt actgattatt gtagttattg ttttaaagat
    13021 ggtgagttta cagctgatat aagtatggaa gagatgatag aagtttgtat ccctcatatg
    13081 cttcaaagta ataaggatat gactgaagat gaagcaagaa caatgatgaa caatttcttt
    13141 ccaacattaa aacgctggaa ataattttat gcaaaattag ttatatgcaa aacatgaaat
    13201 atggtgttga gaataagggg tggtgtgctt tatagcatac cacctgtttt gttacttgga
    13261 gttcagttat tttcctaaat attttaaata tactatttta gataagttcc ctcttcatct
    13321 tgataaatta atcctttttt cataagcact ccaagagctc gtttaaaatt tttcttactt
    13381 gaattaaata atatagaaat atctttaggg tcagatttat cattaaatct catatatcca
    13441 tcagaacctt ctaaataact taaaatctta ttttctaaag tatcaagttc atcctttcta
    13501 tttcctctag gagaaagacc aagttttcca tcttcatata tttttatgac atgtaaattt
    13561 tcaagaacat caccagtttt tagttcagta aagtactcat tatgaagtat tacaccagca
    13621 tatttattgt ctacacatat cattgcagaa ttattagttt ggaatccata tacaactcca
    13681 gttacaatat ctcctacatt gtaagtatgg tctgttaata agtatgaatc tatgtctgta
    13741 gtagcagcaa ttcttttgct tttgtctaaa tatatataaa ataaatattt ttcgtctcta
    13801 aatagtggat aagtcttagc tttaaaagga actaatatgt ctttaggaag tcccatatct
    13861 ataaaagtac caatgtctgt attatcaact acttttaaat gtgctacatc cccaacctta
    13921 gctaaaggcg gtattaaagt tgcagcagtt ctgtcatcag aatctttaaa tatgaaagca
    13981 tttacttcat cacctatgtt tatttcattt tttccgatta atctattatg aagaagtata
    14041 tcgtcttttg tgttattagt ttgtccatcc aaaaaatatc caaattcagt ttttcttttt
    14101 actgtaagtt tattaaaatc acctatgttt atcaaattaa aatcatcctc tcattttata
    14161 tttataattt agccataata tacttatgta gtagtttgtc acagtatcca tattagtatt
    14221 agtgaaattt acaataaata atatagttat ttatgtattt ttcaattctc tagcttctta
    14281 tatagtataa actccaattt cttttttgtc tttaggtttt attaaaattt ttacaaaata
    14341 agttaaaaag tattgaaata aaatttcttt taaagtataa ttggtatata ccaaaattgg
    14401 tatataccat atcgaaaagt ggtgactata tgaaaatagt aaataaatct agtaatatac
    14461 cattgcatac tcaattaagt tcaataataa gagagatgat agaaactggt gagcttaaag
    14521 agggcgatgc aataatgcca gaaagagaac tttgcaacat acaaaatgta agtcgtatga
    14581 cagtaaataa aaccattgtt ggattagtta cagaaggact tctttatagg gtacaaggta
    14641 aagggacatt cgttgcaaag caaaaaaaga agtatcagtt ttcaaatgta aaaggtttta
    14701 ctgatgttat gaaagaaaag ggagtaaaca taaaaacgga tatattatcc tttgagatgg
    14761 aactaccaga tgatttagtt aaaagaaagc ttggaataag tgataatact acaaatatat
    14821 ataagattgt cagacttaga tatactgatg gagagccatt tggattagaa atagtatatc
    14881 tatcagaaga aatgtgtaag ggtctaacaa aagggatatt ggataattct tcattatata
    14941 gggttttaaa tgaaaaatat ggctataaga tacaaaaagc tgaacaggta atggagccag
    15001 taatactttc agatgaagaa agtaagcttc ttgaaacaga tgaaggtgct ttagctctta
    15061 aacttcatag aaattcctat aatagagaag gtagccctat agaatatacc atatcaatat
    15121 ttagaacaga taaatatcaa tatgaaatag tattgagtga gtaggtgaat aatatgaaat
    15181 gtttaattaa tggaaagata atattaaaaa atcaaatctt agaaaataaa gtattagtat
    15241 ttgatgaaaa aataattgat atagctgata gtgtaccaaa agattgtgaa gttatagatg
    15301 ctgatggaaa atatatatct ccagggctta ttgatattca tatacatgga aacatgggaa
    15361 aagatacaat ggattcgact gatgaatcta ttgaaacaat ttcaaagtca attatgagac
    15421 atggagtaac atcttttctt ccaactacaa tgactatgga taaagaacat gtttatgatg
    15481 cacttgaagt tattaaaaaa gcccaaaata gaaagcttga aggggcacaa gtattaggag
    15541 cacatttaga aggacctttt ataaatgaga attataaggg agcacaaaat gaaaaattta
    15601 taataaattc aaaatacgaa tttataaaag aatataaaga tgttataaaa gttataactt
    15661 atgctccaga aaaagatatt gattttgatt ttactagaga aataaaaaga tgtacagata
    15721 tagttttatc tattggtcat agtaatgcaa attatgacca agctaaagaa gctataaatt
    15781 taggagtaac taatgtaact cacatgttca atgcaatgac tggtttaaac catagagacc
    15841 caggcgttgt tggtgcagca cttacaacta atgtgtacag tgagttaata gcagatacaa
    15901 tccatataaa taaagattta tttcaattta tactaaataa taaaggaaaa gaaagactga
    15961 ttttaataac agattctata gaagctggtg gtcttgaaga tggcaattat tctcttggag
    16021 gtcagaaagt catagttaag ggtaatgaag ctagacttga aaatggagca ttagctggaa
    16081 gtgtgttgtc tttaaataaa atggtattta acttcttaga taatacaaac cttaaagtga
    16141 atgaagcaat taatttagct tctttaaatc cagcaacatc tttgggaata aatgacaaaa
    16201 aaggaagctt agaaataggt aaggatgcag atatagctgt atttgatgag aatttagatt
    16261 gtaaaatgac actttgcttg ggggacgtag tatataaaaa tatatagtat acaaaacaaa
    16321 ccttaaagac agtaaaaaca atataaaaaa taataaatta aacttacatt agtaaaagta
    16381 gtagttaaaa tttaggagga ataagatgag agtattagtg tgtaaagatt atgatggaat
    16441 gagtaaaaaa gccgctgaaa tgatagcagc tcaaatagtt ttaaaaccaa attcaatact
    16501 gggactagct acaggaagta caccagtagg tatgtataga gatttagtaa aaaaatacaa
    16561 tgataatata gtagattttt cagatgtgat gagctttaat ttagatgaat attataagtt
    16621 accaatatct aatgaccaaa gctatgatta ttttatgaaa gaaaatctat ttaatcatgt
    16681 aaacataaag ccagaaaata ctcatctacc aaatggaatg gctgatgata ttgaaaaaga
    16741 gtgtatgaac tatgaagctt ctatagatgc agctggtggt atagatgtac aagtacttgg
    16801 aataggtcgt aatgctcata taggttttaa tgaacctgat actaaatttg caaagagaac
    16861 tcatgtagtt gaacttacag agtctacaat agaagctaat gcaagattct ttaaatcaag
    16921 agaagatgtt cctaaaaaag ctgtaagtat gggaataggt tcaatattga agtctaagaa
    16981 aatattgctt ttagcttcag gagaagaaaa agctgatgca gtatataata ctgtttatgg
    17041 agatataact ccagaagttc ctggttctat acttcaactt cataaagaca ctatagttat
    17101 agtagatgaa gcagcagcaa gtaaattgaa tccaaaagat tataaattag tataagaata
    17161 gaatgtagtt taagtaaaaa atagctatca ttgagtaatc aaatttattt aatgatagct
    17221 atttttattt aaatttttta cttcttttta tttaaattag ttattattgt ttaaattact
    17281 tgcttattac tttttacgaa aactcgttat caagaagtat ccaaataaga tgttaaaaat
    17341 tattatgttt agtgggattc tatcaagcat aataaaatta cctataattg ggatattacc
    17401 aactggaaac ataaagaaga atacaggcat cagtgatagt aaaaaagatg gaaaacaaag
    17461 aagaagtaat cttggaatat caatatacca actaccatcc tttttgtatt ctctaatcaa
    17521 taattcagtt cctagtaata aaccaatacc accagaggaa attaaattaa taaaaatgta
    17581 tcttttaaag tcttgcatat aaccagtgta aaattgagta tagaaatcat aatttaattt
    17641 aacagctaaa aaatacataa gcaatagaat gattaaaaat aaaagatatc taagagcttt
    17701 tttcatttta aattctccct tcagaaatac tttattcagt tttattttgt tggaatctga
    17761 ttttatcatt tagtttaaga aatccataat attagtgaag aatgaaataa gtattatata
    17821 tgaataaata tttttagaat taaggtgaat tgtagtgctt aatataacta taatattaat
    17881 tgtttgaata ttcaataaaa agtttgaaaa atatcaattt tgatataaac tgaataaata
    17941 cagttgaata ttaaattatt attttatcaa aaatatgcaa agtactacac cttcaaatat
    18001 cacctaaagt aattaaaagg caattaaggt gaagtagttc aagtatttct aaaataagtt
    18061 taataaattc tataataatt tattttttgc cttaaaattg ccttaaaatc caaacaattt
    18121 taaaactaat gtactataaa aagctggtat aatttaaata tagtaaaaaa taagatttat
    18181 cttatctatt gggatttaat tatttaatca atatatttat accatttaat ttaaataaaa
    18241 agtctattaa ggagtgagct tatgagtaag ttaatttata tagcagatga tgaagataat
    18301 ataagaaatt tggtaaagac attcttaaaa aatgaaggtc atgatgttat ggattttaaa
    18361 acgggtgatg aacttttaga acaatttaat ataaaggagt gtgacttagt aatactagat
    18421 ataatgatgc ctggctcaag tggttttgaa gtatgtacaa aacttcgtga gaaaagtaca
    18481 gtcccaataa tcatgcttac tgcaagagat acagatatag attatattac agggataacc
    18541 ttaggtagtg atgattattt tacaaaacca tttagcccaa tgtctttggt tatgagagta
    18601 aaatctatat ttagaaggat agagtttgaa aagaaacaaa attatgataa atatagtaac
    18661 tcgatagata tggaattaaa atttggtgat gtaataataa ataaaaagaa taaaatcgtt
    18721 acaagtaaaa atgtaaatat agaccttaca ccaaatgaat ataacctatt gacttatttg
    18781 tttgaaaata tagatagagc agtttccaga gatgaattat taaacaaaat atggggatac
    18841 gatatagaag ttgagacaag ggctgctgat gatacagtga agagattacg taaaaaaatt
    18901 ttaaatacaa atattttaat agaaactgta tgggggtttg gatttaggtt gaaggagaaa
    18961 agttaagatg aagaatggaa atgacaagaa tagaattgga aagattaaat ccaaatttaa
    19021 attaaagttc tcaaagaaaa atataagcat aaaaactagg attttgttga gtatactttg
    19081 tttgataatt atagtattta tgataatatt agtatctttt aatgtatttc ttgatacata
    19141 tataaaaaca actgcgaatg aagaattgac aaagtctaca gaaatagtag aacatatgga
    19201 tagtaatttt agacctatta agccaccaca agatgatgga aagcttcctc ccaaagtatt
    19261 atctaatttt atgagaaatg tacaagataa agttaggatg gcagaaacac aatcagatgc
    19321 tgatgctatg gtagttgatt ctaagtataa cctgatattt ccaacaagag aggatgattt
    19381 cttaaaaaat gttgatgaga tggagctgat tagggagtgt attcaaaatg aaaaattaga
    19441 tttaaattca gatgaaaata cacggatatc taccagaaat aaggattatt atatttcaac
    19501 tgtaaaaatt actagtatat atggtgaaca agacaagtat cttatacttt ttatagacat
    19561 atcaaaaact ctaaatctag cacaaaagat aaatattgta ttaatatctg taatgtgttt
    19621 tgctggaata ttagcaatat ttacagctgt gattttatct gagaaaattg ctaaaccgat
    19681 aaaagagttg tgtgagtttg caaaaaaaat gggtcaagga gatttcaaaa gaacctactt
    19741 tgacttttca gataaagaat tagtagagct ctcaactgtt atgaataaga gtgctgagta
    19801 tctggataag tatgataatg aacaaaaaat attttttcaa aatgcatctc atgaattaag
    19861 gacaccactt atgtctatta aaggttatgc agaagctata aaatataatg ttatagatag
    19921 taaacatgca agtgatataa ttcttgaaga aagtgatagg ttaagtgata tggttgaaga
    19981 tttattatac atttctaaaa tagataatat aactaaagat tttgaacttg tagaatgtga
    20041 tttaagagaa gtactttcaa attgtggagc taggcaaaat gtaagagcta taaataaggg
    20101 gataaaattt atttatgaat ttgatgaaga aatggtttta tttgaatgtg atgaaaaaaa
    20161 catttccaaa gcatttatga atcttataga aaatgctttg agatacgcta agagtgaaat
    20221 aaaaatagtg tgtaagtata atcaaaaaaa tatagttgtg ataatagaag atgatggaat
    20281 tggaatcaag aaagaagatt tacctcatgt ttttgaaaga ttctataagg gagtaggggg
    20341 aaatcatgga attggattat caatagttaa atctattgta aataagcatg gtgggcgtat
    20401 atacgtagaa aatggcaaaa aaggagctaa atttactata ttatttaaat tgtaaaaaaa
    20461 ttagagaaaa tatagagatg tttgaagttg ttttttatat atatctgttg tatactgttg
    20521 aaaaaagaac aatattaagc tgacatatat tacgatagtt gacatttaaa gtaaatgctt
    20581 aaatatagat acgaaaggaa aagtaaaatc tgaagtgtat ttaggtttaa gtaagcttct
    20641 tattttataa aaaatatgta tgaaagaaca gctgattttt aatattacaa atttagtaac
    20701 ggttgtattt gaagcttttg tcatccatat gtttttatca gactttttag gtcagaaaga
    20761 agggtatata aaagttgtaa gatatgctaa actgggtttt ataatatgtt taggattctg
    20821 taatttaata actttaaacc ctaaaatcac aatgccttta attttcatcc ttattttttc
    20881 aacatcattt ttatataaag gaaatttaaa gacacgattg tttactactg ttttattaag
    20941 tatattcttt atactgtcag aaattgtagt tacaagtatt tttgttttat ttgtaaaaga
    21001 aggctttgaa ataatgcttg aaaataatag tataagagta ttggcgacaa ttttatctaa
    21061 aattgtcttt ttgttgactt gtaaaattat ttgtctattt aaaaaggatg tacatttaga
    21121 tatgcccata aagtattggc tccctttatt tttaatacct atatttagtt tatttctgtc
    21181 tgtttctatc tttgatgtaa gtaaattttt tagtcttgaa agtttaaaat ttctaagctt
    21241 aatatctagt gttggtattt tatatattaa ttttattgtc ttttatttat ttaaatttat
    21301 tatagataaa actaaactat ctatgaaata tgaactactt gaaaaagaaa taattcataa
    21361 ggaagaatta agacttagta atgagtgtta taaaattatt gttgaacaga ctgattctgt
    21421 cgttttcgaa tggaatatca aagaaaataa atcatttgta tcgcaggctt ggactgaaaa
    21481 atttggatat aacaatgcat gtaagaatat ttttaaagaa attaaagata aagacttagt
    21541 tcattctgaa gataaagcta tatttgaagg ttttcttgaa agtattaaaa aaaagaatat
    21601 gcacaatcaa gcagtatatc gtttaaaaaa atctaatggt gaatatattt ggtgtagaac
    21661 atcaattaca agcatttata atgatgaaaa tgaactttta agagttgtcg gagtaattgt
    21721 agatgtagat agtgatataa aaaaatatga agagttaaga actagagcag aaagtgactc
    21781 attgaccaat atatacaata aaggtacatt tgaaaaatta gtggaagaaa ctatagttat
    21841 gaatacaggt gataaaaaag atgcattatt cattattgat ttggatgatt ttaaagaaat
    21901 aaatgataat tttggtcatc catttggtga ttttgtacta aaaacatttg ctgataagat
    21961 acaaactagt tttggtagta aagacttagt cggaagaatt ggtggagatg aatttgttgt
    22021 atatatgcaa gattatgtta cagaagtaaa tttgcataaa aaagctaaag aattaaatag
    22081 agtactttcg gataattaca ctgatttgag ttttagtttt gatgcttctg tcagtatagg
    22141 tattgcaagg tatcctcaag atggaacttc attttttgaa ttgtttaaaa atgcagatag
    22201 agcactttat agcataaaag catcaggtaa aaattcatat tgtttatttg aagaagaact
    22261 atatgtacag tgaatttgaa tttagatttt atgtgagagt aagatttata tatacttgtg
    22321 gctgtctgat ttataaaata tgttactttg tatattttta tatgtttcag taaggttaat
    22381 taacatgagc aaaggtgtaa gtaaatttat attagtttaa gtttacttac acctttataa
    22441 aaatacaaat aatagattca tttacattct taaatgatta aagctcttta ccttctaaac
    22501 atgcataaca tatcgttata attccagtaa ataagactaa aatgcccatt actaagatta
    22561 caatttcatc atctattact ccaaatgttt ttaaagtaac ttatttcgct ttgtatcata
    22621 ataatttgtc tctttttaca ttttaacttc tgctgagatt tgagttagat attcattaga
    22681 atctcgcaca cttatatcat atagaagata ttcttcataa acaaaatccc caatacgtaa
    22741 attattctga ttaaaaaaat ttaataactt ttcataagcc ttgtaaatag tttcataact
    22801 ccctttatga taagcagtta cataaagtcc ctttggtttg attgatacag gatatttctt
    22861 gtactcttta ttaacttttg taaataaata tgaatatgat tcaaagtctt tattaattat
    22921 atcttcacct ttaatgattg agccaatcgt accataatca ctcaattcat agttgtccat
    22981 aaagttaata cattcatata aaaaattttc ttcctcatct cctaaaaact gttctttata
    23041 tgtaacaggt gttttgacca agtactcttc ttcatgttct tttaaagtaa tagtattcgc
    23101 atctatatta catgcacttt tagtaaatga aatagtagat tccattagag cttgaatatt
    23161 atttagtttt tcaatttcat ttttaatatc aatggttttc ttttctaaaa gctcaattag
    23221 taaattaggt gttcttttgt ctatataagc tttaatttct tttaaaggca tttttacttc
    23281 cctaagtgaa gaaattatac caaaaatgct aagttgatgg tatgagtagt atctatatcc
    23341 attttctttc tttaattctg gtgagaaaag acctatatca tcataatgga aaagtgtttt
    23401 tttattgatt ccacaaatct ttgcaaattc tccagttgta aaataatttt ttgaaacttg
    23461 actcataatt ataattcctc cttgactatc ctgttacagt atagtttatc atagtatttt
    23521 gtaaaggagt gtgattatat gaaaaaatta attcattttt taaaaccata tagagtttta
    23581 attgttgtag ttttgatatt tacgttttta caaacacttg gaactttgta tataccaaca
    23641 cttacagcaa atatagtaaa taatggtgta gtaaagggag atatagatta tattgtaaaa
    23701 acaggtttaa tgatgatgat agtagctggt ataacagctt tatcagctgt gttagtttgt
    23761 aaagtatcag caaatctttc atctggattt tgtagagata ttagagaagc agtatttata
    23821 aaatcacaag atttatcaat aaatgatttt aataatatag gtacagcatc aatgattact
    23881 aggagtacta gtgatattac tttaataggt cagtctgtgt ttatgtttat tcaattagta
    23941 ttaccagcac caattataac tgtttctggt ctattcttag catattctat agataaagca
    24001 atgacaatta ttattgttgt agtaatgttt ttattcatgc tttcagcatt tttagttggt
    24061 aaaaagctta taaagttgtt taaaatgatg caaataaaga tggataatat gaatagggtg
    24121 ttacgtgagg tagtaactgg agtaagagtt ataagagctt ttaataggag tcgttttgaa
    24181 aaaaagagat ttgatagaac tgcaatagat tattctgaaa cagcaatttc aataaataaa
    24241 atatttgcag tacttatgcc tattgtaatg ttaataatga atttaggtat agtaagtatt
    24301 atttggtttg gtgggatgag agtcagtaat ggaaatatgg aaataggtca tataatggca
    24361 cttgttgaat attgtatctt aattttattc tatttaatca tgggagtaat ggtatttatg
    24421 tacataccta gagcaggtgc atgtgcagat agagtaaatc agatattaga tatagaacct
    24481 gaaattgtag atggaaatgg tcataaagat actgtctcag agagaggaca tttagtattt
    24541 aaaaatgtaa ccttttcata tgcacaatca gaagaacctg tgttaaataa tataacattt
    24601 gaagcaaaat ctggagaagt aactgcaatt ataggaagca ctggctcagg caaatctact
    24661 attgcaaata taataccacg tttttttgaa attcaatctg gagaaatatc tataaatggt
    24721 caggatatta agaagattcc tcaaaaagaa cttagagata agattggatt tgtaccacaa
    24781 aaagcttttt tatttagtgg aacaatagaa gaaaatataa ggtatggaaa agaagatgca
    24841 agtattgaag aagtaaagca tgctgcaagt atagctcaag ctgatgaatt tatttatgat
    24901 atggaagaca agtatgactc atttgtagca caaggtggaa ataatttgtc aggtggtcaa
    24961 aaacagagaa tatccattgc aagagcgtta gttagaaagc ctgaagtata tgtctttgat
    25021 gatagttttt ctgcattaga ttttaaaaca gataaaagac ttagaaaagc gttgaaaaat
    25081 gaaatcaaag attcatcagc tataattata gcacaacgta taagtacaat catggatgct
    25141 aatcagatta tagttttaaa tgatggaaaa attgtaggta ttggaaaaca taaagattta
    25201 cttgaaaatt gtgaagttta taagcaaatt gcggattcac aacttagtaa agaagaactt
    25261 gcataaaatg aagaattaat ctgatatttg gaggtgtatg atatgagtga aaaaagaata
    25321 gatgaacaag atatagaagt acttagtttt gaagatggca tgtcagcaga aaaagctaag
    25381 gatgcaaaaa aaactactaa gagattatta aaatatatgg caaaacaaaa gtttaaaata
    25441 ttaattattt ttatttcagt attgataagt tcagcgctta cagttttagc acctatggtt
    25501 atgggaaaag ctattgacca actatttaat ggaataaaaa ctgcagtcca aacaggtaca
    25561 aaattcagtg tgaattttag tactatggga gggatagtat caatacttct agggttgtac
    25621 ttaattagtg cagtatttat atatattcaa cagtatatta tgtcaggagt tgcacaaaat
    25681 ctagttctta gcatgagaaa agatttaagt gataaactca ataaacttcc tcttaagtac
    25741 tatgattctc ataaaaaggg ggaaacactt agtatagtaa caaatgattt agaaaaagta
    25801 gcagatagtt tacaagaagg attaatgcaa cttattacag cagtagttac agtagttggt
    25861 tctattgtaa tgatgataag tataagtgta ccacttacaa tagtatcagc tataacatta
    25921 cttgtttcat tgggtattac tgttgtaatt gcaagaaaat cacaagaaag attctctgaa
    25981 aatcaaaagg cactaggaga gttaaatagc aatatagaag agatatttac tggtcaaata
    26041 gtggttaaag catttagtaa agaaaaggat actataagaa actttaaaat attaaatcaa
    26101 aatctttata atgcaagtag aaaagcacag ttttcgtcat atgcaatatc tccaattatt
    26161 agatttataa acctaattgg ctatgttatt atagcagttg taggtgggat atttgcatca
    26221 actggagcaa tgactcttgg tagtatacaa gcgtttattc aatatgtaaa tcaagcatca
    26281 gagcctacta cagaaatatc ttatattgta aatatgcttc aagcagcaat agcatctgca
    26341 gagagagttt ttacagttat ggacgaggtg gaagagataa aggatgaaga atcttcaaag
    26401 gttattagca tgcctaaagg taaagtacaa tttgagcatg ttaaatttgg atatagtgat
    26461 gattttattc ttatgaaaga tattaatatc aatctaaatg caggagataa gattgcaatt
    26521 gttggaccta caggagctgg aaagacaact cttgtaaatt tacttatgag attttatgag
    26581 atacaaggtg gaagaattac tattgatggt gttaatataa aagatttaaa gcgtggagaa
    26641 cttagaacta tgtttggtat ggttcttcaa gatacatggc tgtttaatgg aagtataaaa
    26701 gaaaatattg catatagtaa gtcagatgcc acaatggatg aaattgtaag agcagcaaaa
    26761 tcagcaagag tagaccattt tataaggact ttaccacaag ggtatgacac tatattgact
    26821 gaggatgctt ctaatctatc tcaaggtcaa aagcaacttc taacaattgc cagggcaata
    26881 ctttcagacc catcagtatt gattcttgat gaagcaacat ctagtgtaga tactagaaca
    26941 gaggtagaaa ttcaaaaggc aatgaataac ttgatgaagg gaagaactag ttttgttatt
    27001 gcacatagat tatcaactat tcgagatgct gatttaatat tagtaatgaa agaaggaact
    27061 attattgaac aaggaagcca taaggaactt attgaaaaga aaggattcta tgaagaactt
    27121 tataacagtc aatttacaag tgaatatgat gaagatgttg tatgattatc aagttttagg
    27181 caactaaatg attgataaat attaagttaa atatagtaac atgtaaggtt atatggaata
    27241 agagtatttc tctatttcat ataacctttt ttatttatca aaataagata aaatttcctt
    27301 tctaaattag attgaagaat gtatttatca atataaaagt aaaataatca ttattattag
    27361 tgaagtaaat attgtttgcc acatatatgc cataaagatg cacttatttt acggagattt
    27421 aacaaagtag tgtaaagtat actctaaata taaaataaat caaatagagt taaataaatt
    27481 gagaggtgat ataaatagtg aataaaaaat tgaactatag atttgaaatg aagcacaaaa
    27541 taactgaagc agatgtgtta gctttaaaat caagactcta tccaataatg aagaaagatg
    27601 aaaatgcatc taaagatgga aaatatctta ttaggagttt atattttgat actcctgaag
    27661 ataaagcact tttggataaa ttaaatggcg ttgcaataag agaaaagttt aggataagat
    27721 tttataataa tgactgctct tatataaggt tagagaaaaa aatcaaacat tacaacatga
    27781 catcaaagct atcaactaat ttaaccaaaa aagaagtaat aaatatacta aataatgata
    27841 ttgaatttct aaaagagtcg acaaattgtt tattaagaga attttatttg aaattaaaat
    27901 gtgaaagatt agaggcaaaa agcatagttg attacaatag agaagctttt atttatccag
    27961 ttggaaatgt aagagttaca atcgatagtg atattaaaac ttctgtaaat tcggtagatt
    28021 tatttaataa agatttgcca actatttcgg taattgatga gaatatgact gtattggagg
    28081 ttaaatatga tgaatttata ccagatttta taaaggattt aattcaaatt aataagacta
    28141 cctctacagc agtttcaaag tatgcttcaa gtagattata tatttaagaa taaaggctat
    28201 gatttatata aagatataca taaataaggt taaaaaacaa ataagttaaa aataaataaa
    28261 ataaaaaata aataagttaa aaataggaag gatgatttta attatgacat ttaatgatat
    28321 ttttaaatca agttttatag aaaacgtatc aggtttttca tttgtggaca gtgctttagc
    28381 actaggttca gcttttttag taggtttgtt tatatatatg gtctataaaa agacttatat
    28441 gggaattatg tattctcgac cattcaatgt gtcgcttgta gcacttacaa tgcttacaac
    28501 ttttgtaata ttggcggtta cttcaaatgt agttttatcc ttaggtatgg ttggtgcttt
    28561 atctatagta agatttagaa cagcaattaa agacccaatg gatttagtat ttctattttg
    28621 gtcattaggt tcaggtatag ttttaggagc aggtttgatt cccttagctg tcatgggttc
    28681 aataattatg ggattgattc taatattctt ttcaaataaa acaatatctg aaactccata
    28741 tatattgatg gtaaattgta ataatgaaga ttcagaagat atagcaactg ataaaattaa
    28801 aaaagtattt actaaatatc aaataaaatc aaaaagtgtt acaccagaaa aaggaataga
    28861 attagtgttt gaagtaagaa tgaaagatgg agaaacaagt cttataaatg acttgagtca
    28921 agtagatgga gttactaatg cagttcttgt aagttataat ggtgactatg tagcatagag
    28981 aggagtaatc ttttatgaaa gataaaaaat ttacccttct tatctcgatt atgattatat
    29041 ttttatgtgc tgtagttgga gtttatagta catctagcaa caaaagtgct gatttatata
    29101 gtgatgtata tattgaaaaa tattttaaca gagacaaggt tatggaagtt aatatagaga
    29161 tagatgaaag tgacttgaag gatatgaatg aaaatgctat aaaagaagaa tttaaggttg
    29221 caaaagtaac tgtagatgga gatacatatg gaaacgtagg tataagaact aaaggaaatt
    29281 caagtcttat atctgtagca aatagtgata gtgatagata cagctataag attaattttg
    29341 ataagtataa tactagtcaa agtatggaag ggcttactca attaaatctt aataactgtt
    29401 actctgaccc atcttatatg agagagtttt taacatatag tatttgcgag gaaatgggat
    29461 tagcgactcc agaatttgca tatgctaaag tctctataaa tggcgaatat catggtttgt
    29521 atttggcagt agaaggatta aaagagtctt atcttgaaaa taattttggt aatgtaactg
    29581 gagacttata taagtcagat gaaggaagct cgttgcaata taaaggagat gacccagaaa
    29641 gttactcaaa cttaatcgtt gaaagtgata aaaagacagc tgattggtct aaaatcacaa
    29701 aactattaaa atctttggat acaggtgaag atattgaaaa atatcttgat gtagattctg
    29761 tccttaaaaa tatagcaata aatacagctt tattaaacct tgatagctat caagggagtt
    29821 ttgcccataa ctattattta tatgagcaag atggagtatt ttctatgtta ccatgggatt
    29881 ttaatatgtc atttggtgga tttagtggtt ttggtggagg tagtcaatct atagcaattg
    29941 atgaacctac gacaggtaat ttagaagaca gacctctcat atcctcgtta ttaaaaaatg
    30001 agacatacaa aacaaaatac cataaatatc tggaagagat agtaacaaaa tacctagatt
    30061 cagactattt agagaatatg acaacaaaat tgcatgacat gatagcatca tatgtaaaag
    30121 aagacccaac agcattttat acttatgaag aatttgaaaa aaatataaca tcttcaattg
    30181 aagattctag tgataataag ggatttggta ataaagggtt tgacaacaat aactctaata
    30241 acagtgattc taataataat tctaatagtg aaaataagcg ctctggaaat caaagtgatg
    30301 aaaaagaagt taatgctgaa ttaacatcaa gcgtagtcaa agctaataca gataatgaaa
    30361 ctaaaaataa aactacaaat gatagtgaaa gtaagaataa tacagataaa gataaaagtg
    30421 gaaatgataa taatcaaaag ctagaaggtc ctatgggtaa aggaggtaag tcaataccag
    30481 gggttttgga agttgcagaa gatatgagta aaactataaa atctcaatta agtggagaaa
    30541 cttcttcgac aaagcaaaac tctggtgatg aaagttcaag tggaattaaa ggtagtgaaa
    30601 agtttgatga ggatatgagt ggtatgccag aaccacctga gggaatggat ggtaaaatgc
    30661 caccaggaat gggtaatatg gataagggag atatgaatgg taaaaatggc aatatgaata
    30721 tggatagaaa tcaagataat ccaagagaag ctggaggttt tggcaataga ggaggaggct
    30781 ctgtgagtaa aacaacaaca tacttcaaat taattttagg tggagcttca atgataataa
    30841 tgtcgattat gttagttggt gtatcaaggg taaagagaag aagatttata aagtcaaaat
    30901 aagattaaaa ttgaatataa ataattttaa tactgttaag tctaaatatt tacttagcag
    30961 tattttttta gaaaataatg tatttggtta ataatataag gtaataaatt gataaaaatt
    31021 aaatacagca tattaataat acataataaa gtaataaaaa aactgtttaa gtataataac
    31081 ttatatttag acagtttttt tcatttataa ataatataaa tgaaattgaa aacatttgaa
    31141 ggatagaaat tggttggata aatgtataaa ttacttcacc aaaaaataat gacaaaaatt
    31201 gtaactaagt tgtaataaaa tggtagttat ttagcataaa ataatgtatc atagaagtat
    31261 attataaaat taataaaata agatttttgt aagaaaactg ctggcttttg atatttagag
    31321 tgagtagtta aattatgttt aggaggggta gaatttgttg caaaaagtat tatggaaatt
    31381 tttaaagcta gtagtcggtt tatttatatg ttcagttggt atagtattga cgataaattg
    31441 taaccttgga ttatctccct gggatgtatt tcatcaagga ctttcaaatc atataggaat
    31501 aactatagga actgcttcta taattgtagg ctctatagta gttattgctg atgtcgttct
    31561 tggagaaaat gtaggatggg gaactgtttt taacatgcta ctaattggat tctttatgga
    31621 tttactttta tatagtaatt taattccaga ggctgatagt ttatttgtag gaataattat
    31681 gttgatatta ggattggttc ttttgtctgt tggaatggta ttttatatgg gaagtggttt
    31741 aggaagtgga cctagagatg gtttgatggt tgcaattcaa aagaaaacag gaaaatcact
    31801 taaattaata agaggtacta tagaagttgg agcattaata gtaggatttt tattaggtgg
    31861 aaaagttggt ataggaacta taataagtgc atttggattg gggtatttca cacaaatggt
    31921 atttggctta tttaaattag attgtagtaa gattaaacat agatttatag tagatgatat
    31981 aaaatttatc aaagtgtata tgagtggtga gaaaaaagct gctgttcaaa atatagttaa
    32041 gtaatttaag aataaattca acgaaatgtt aagtatttta aattaacatt ttgtttagat
    32101 agataaattg aatattaaaa gaatattaaa atatgaggat tgctagaaca ctataaaata
    32161 tagatttcta gcaatttttt atttgttcaa tattttttta gtactagaag tcttattctc
    32221 tatttaatga atgaattaat ttataattag agatactaaa ctttatagat attaatataa
    32281 acacctttaa actgtgtatt ttccaaagcc tttaaaatgc ttgtatttat tctaaataaa
    32341 aaaataaaaa aatgtcaaaa aaactcttgt attatatcca aattttatat ataattacct
    32401 atgttgttta gaaattttaa actcaatgtt tagtaaacaa aatgtttagt aaaataaaac
    32461 agaaactagg aggtgcaaca tggatatagg tgaaaagata aaaagattaa gaacagaaaa
    32521 acaactgacc caggaagagt tagctaatag atgtgaattg tcaaagggat ttatttctca
    32581 attagaaaat aacttaacat caccatccat agctaccctt atagatatac ttgaaatact
    32641 aggtacaaat ttaagggaat tttttaatga aatagatgat gaaagaatca gttttacaaa
    32701 agaagatatg tttgaaacag aagatgaaga tttaaaatat aaattaaagt ggttaattcc
    32761 aaattcacaa aaaaatgaaa tggagccaat aataataaca ttgtatccag gaggacagta
    32821 taaagaagaa aagccacatg aaggtgaaga atttggatat gtattagcag gttcaatata
    32881 tgtccatata ggcgaaaaga aaaataaagt aaaaaaagga gagagttttt actttagacc
    32941 gaaagcaaat cactatatat caaacgaggg caaaactaca gcaaaagtga tttgggtaag
    33001 tacgccacca tcattttaga atagaggtgt ttaataattg gtagaaaata taatagaatt
    33061 aagggatata tctaaacatt atgaagaact tactgtttta gataatttca atttagatat
    33121 aaagaaaaat gagtttctaa cattacttgg accaagtgga tgtggaaaaa ctacaacatt
    33181 aaaaataata gcaggatttg agtatgctga tgatggtaaa gttttatttg aaggtaagga
    33241 gataaataat ttgccaccat atgaaagaca agtaaacaca gtatttcaaa aatatgcttt
    33301 atttccacac atggatgttt atgaaaatgt agcttttgga ttaaaaatta aaaagactcc
    33361 aaaagatgta atagatgcta aagtaaaaga ggttttgaaa cttgttgcac tagaaggttt
    33421 tgaaagacga caagttgaat cattgagtgg aggacaacaa caaagaattg caatagcaag
    33481 agctctagtt aatgaaccaa aggtattatt actagatgaa ccacttggag cacttgattt
    33541 aaagttaaga caagagatgc aaatagaatt aaagagaatc caaaaaaaat taggaataac
    33601 atttgttttt gtaactcatg accaagaaga ggctcttact atgtcagata ctattgttgt
    33661 aatgtataag ggcaaaattc aacaaatggg aacaccacaa gatatataca atgaacctaa
    33721 aaattcattt gtagctaagt ttataggtga aagtaacata tttgatggaa ttatgataga
    33781 agattataaa gttaatttct gtaatagaga ttttgagtgt gttgataaag gattcgaaaa
    33841 aaacgaaaat atagaagtag taataagacc agaagatata aaaatggtaa agccagaaga
    33901 aggaatgtta aaggggaaag ttacatctac agtgtttaaa ggagtgcatt acgagataga
    33961 attgaatgaa aatggaagat tgtggatact tcataatact aaaaatgctg aggttgggac
    34021 tgaactaggc atggatatat atccagaaga tatccatata atgagaaaag agaataacta
    34081 agatgaaaag aaaatctttt ttagcatatc catatgtagt ttggagtgcg atatttgtaa
    34141 taattccttt aatattagta gtatttttta gttttacaaa agaatcaggt ggaggttatg
    34201 cctttacact tgagaattat aaagaagtaa ttgaccctat atatatgaaa gtttttgggc
    34261 gttctattct tttagcagga ggagctacat taatatgcct tatagttggt tatccagtgg
    34321 catatataat ttcaaaggct cgtgtaagta gaagaggttc attaatatta ttgtttattc
    34381 ttcctatgtg gatgaacttt cttttaagaa catatgcatg ggttgctata ttaggaaaaa
    34441 atggactttt aaatacattt ttaggttggt ttggaataca accattagca attttgtata
    34501 ctaattttgc catactttta ggtatggtgt ataacttttt accatttatg gttcttccaa
    34561 tatatacagc actatcaaag atggataatg atttgataaa cgctgctcat gacttgggtg
    34621 caaataatat gacagttttt agaaaaatta tatttccttt gagtttgcca ggtgtaatgt
    34681 ctggaataac tatggtattt atgccagcag tcactacttt tgcaatatca agactgttag
    34741 gtggaggtaa aataatgctg gttggagatt tgattgaaca acagtttaca gttgttggag
    34801 attggaactt tggttcagct atatcgatat ttatgatgat agtaatatta atatctatgt
    34861 ctataatgtc aaaatttgga gatgaatctg acaaagaagg cggtgggtta ctattttaaa
    34921 attaaagaaa ataacttcta atatatattt agcattagta ttcttatttt tgtatgcacc
    34981 catatttgct ttggtactat tttctttcaa tgattcaaaa tcaatggcaa gatggaatgg
    35041 atttacatgg aagtggtatg gtcaattact tcaaaatgag agtataatga gtgctttata
    35101 ttatactatt gtaatagcta tattagcgtc tgtaatatct acaatagtag gaactataag
    35161 tgctatagga atacataaga tgagaggtaa gagtaagaaa ttaatactaa atgtaaacta
    35221 tcttcctatt ttaaatacag agatagttac agcagtagct cttatgagtc tgtttgtatt
    35281 tgtaaaaatg gaatttggat ttacaacaat gctattggca catataatgt tttgtttgcc
    35341 atacgttata ctttcagttt tgccaaaaat gaagcaatta cctgataata tagaagatgc
    35401 agctatggac ttgggagcaa cacctatata cgctcttaga aaagtaattt taccacaaat
    35461 aaaaccaggt atagtttctg gatttttaat tgcatttact atgtcaatag atgactttat
    35521 aataagtttc tttaatgctg gaaatggtgt aagcaatctt tctatagaaa tctatggaat
    35581 ggctagaaga ggtataaaac ctgaaataaa tgcattatca acaataatgt ttgcagtggt
    35641 tctaggttta cttttactag ctaataaaaa agaatctata gtaaggggga ttaaataatg
    35701 attaagttta aaaaagcaat atcccttata gctgtaggta tgatgacagt tactttattt
    35761 acaggatgta ataaagcaac tgattctaca aaagttttaa atgtatataa tgttggagat
    35821 tatatagatg agtctcttat agataagttt gaaaaagaaa caggaataga tgttcaatat
    35881 tcaacttatg atactaatga aatgatgtat caaaaagtaa agagtggtag tacacactat
    35941 gatttagtat ttccttcaga ctatatggta gagaaaatga aaaatgaagg tcttttggaa
    36001 aaattagatt ttaagaatat acctaatatg aaatatttag ataagagttt tttaaatcct
    36061 gtatacgaca aaactaatga atatagtgtt gcatatatgt ggggaactct tggaatactg
    36121 tacaataaga aagaagttaa agaccctatg gatagctgga atatactttg gaatccaaag
    36181 tataagggaa atataatgat gtttgactct gttagagata ctatagggat aactcttaaa
    36241 aaattaggat atagcatgaa tagtgtaaat ccaaaagaaa taaatgaagc taaaaatctt
    36301 ttaatgaaac aaaaggattt agtattggct tatgtaaatg atgaaggtaa ggatagattg
    36361 cttggtggag aagtggcaat gggtatgctg tattctggag atgcagttac actaatggag
    36421 caaaatcctg accttgatta tgctatacct aaagagggaa ctaataaatg ggtagatgct
    36481 atgtgtgtac ctacaacagc acaaaataag aaagaagctg agctgtttat aaacttttta
    36541 ttagacccag agaatgcaaa agtaaatgct gagtacattg ggtattcaac acctaataca
    36601 ggagcactaa aattattaga cccagaaata actcagaatc ctgttgcata tccatctaaa
    36661 gaagtgcttg ataaatgtga aacttttgaa gatattggtg aaaatataaa attatatgac
    36721 aaggcttgga tagagttaaa atcaaaatag ggtcttgttg gtttaagtaa atttacttaa
    36781 taaatactta ataaaactgt tcttttttag aaaatttatt ttctaattat tgaacagttt
    36841 tattttttgt ttaagttata ttcatatgca atttaaatag tttattaata aatgaaaact
    36901 tatttgtata atgactttaa taataattag aaaaaatggg caacttaata gaataattgg
    36961 aggtgtaaat ttataaaaaa tgaataggat aaataaaaaa atagatttat taatattggc
    37021 tttgctcgtt tttatatttt taattgttgg aatagtaata ctatcgatta ggacagaaaa
    37081 taaattgaat gtgttcataa tgttagcaat aatttttttt ataataatgt taacttattt
    37141 atcaaattca gtagtggggt tgataacaag ttcaataatt atatttatgt atacatccta
    37201 tattttatat aacaatatta ctcacaatat ggatgttgaa tttatatcat atatgtggat
    37261 aatagcaaca ccagtatctt caattataat gggtaatctg aacaaaagca taaatgaatt
    37321 gcaaaataca aataaaaaac tatctgaaca gtataaggaa ttagtgacta ttgatagtga
    37381 aactggactt agaaatttaa agatttttta taatgatgta aatatggaga taagtaaatc
    37441 tattagacat aatactgatt tttctcttat gatagtaaaa ttaccatact acggaaacct
    37501 acagactata tttggtgaaa acaaaactaa taaaatagta aaatatattg gttcaaatat
    37561 tatagaatgt acaagaaatg aagatataat ttatagttta caaaaagata tgatagggat
    37621 actaatgcca aatacatctt tggaaggttc aaaagttgtt aaagacagaa taaagaaaag
    37681 gataaaagaa ttaaatttag atttaaacaa taggggaaaa tatgtaaata tagatgtcaa
    37741 aattgcattt ttacaatata agagtagttt tggagatagt ataaatttta agaatatagt
    37801 tgaagaggaa cttcaatatg atgtttaaaa tatttaataa aagtctatta aaattagtag
    37861 ttgtattatc aatagtactg acaatatcaa attttagctt atcttcatat gcatcagaaa
    37921 ttacttccag cacagataag gtattaatca tatatgattc taagaaagaa acagcttata
    37981 atagagatat tttaaatatt atgaggactc tattgggaag atttagttca gatatagagc
    38041 tattaaaatt gagtaactat gatggtgaga taaataaaaa ttattacagt catatattta
    38101 ttttaggaat caatgaaaac tcatataata atgataaaaa cactaaaaac ttgattagtt
    38161 ccttaaattt atataaagga actatatgtt ggttaggata tggtatagaa aatcttttgg
    38221 aacataaaaa atacaattta gactatgtag gaaaaacaaa caatatagtt agtgtaaatt
    38281 atagaggtaa atcatataac ttggatgaac attatgtttt taacattgta gaatcaaaag
    38341 atacaagtaa taaagtaata ggaagtatta atgatacctt aaataagtat ccatatataa
    38401 taaatgataa aaatcttttt tatgtatcaa aattagattt agatggagtt ttattttata
    38461 tattttgcga ttcacttaat gatattttta atattaagac gtttgataaa ggacgtatat
    38521 ttgtaagaat agaagatgta catgctttta gagaaccaaa aaatcttgta gagatagcgg
    38581 attatttaag tagtaagaat ataccattta cgattgcatt aataccagca tatgtaaatc
    38641 ctaaaaatca taaggtaata actttatcag aaagccctga aattgtcaaa gcaataaaat
    38701 atatgcaaga taaaggtgga actgtgatat tgcatggata tactcatcaa tacaagaaag
    38761 aagaagtttc tggagaaggg tatgaatttt gggatggtaa aaaagatgaa cccttaaaag
    38821 aaaatatgaa gatatttgtc aaagatagag ttttgaatgg tcttagagta tgtatagaga
    38881 atggaattta tcctcttgca tttgaagcgc cacattatgc aatggaatca gagggatata
    38941 aagaattaaa aaaatatttt tctacttata tagggcaaca tcaaaataat gataaaaaat
    39001 ttagcacaaa tacatatcct tatattatta gggatacaga ggagtttaat atatttatac
    39061 ccgaaaattt aggatatata gacccagaag ataagtttac ttttcaaaat ataaaagaaa
    39121 atttagataa attatccatt gtaagaggtt ttagtggagg gttcttcttt cattcatatt
    39181 taaacataga gtatctaaaa aacacaatag aatatttaga aaaacagaat atagaattta
    39241 tgaatttaag agattttaat aattgggtaa aagttgatga aatacaaata agaaataatg
    39301 gagatgaaat tatagtcaac tatgataagg acttagatga actaactaaa agtgatacta
    39361 gatttaaatc catatccaat attagtaaag ttttaatatt catagtatct ataagtgttt
    39421 taatatttgt gattatattt atatatttta agagaataga taaaaagaaa tttttaaaat
    39481 aaaggatttt atattagaat caatatttta aaatatttta ggagaaattt atgagagatt
    39541 taaatgtatc agagtatttg ttcgtattta gtttgtttag tatatggtct ttattgctta
    39601 taaatattat tttagctatg ggtggatata tattttattt taaaaacttt gataaagaaa
    39661 taaaagaaat tgatgaatat cctatgatat caatacttgt tcctgctcat aatgaagcta
    39721 aagtgatagg tagaactgtg gagtctttgc tattactcaa ttatcctaag agtaagatgg
    39781 aacttattgt tataaatgac aattcttctg ataatagtaa agaaatttta gaaaatataa
    39841 aggataggta caacaattat aatttcacta taataaatac agatagttta acaggtggaa
    39901 aaggaaagtc taatgcatta aatattggat atacgatttc aaaaggagat tttatagctg
    39961 tttatgatgc agataacaca ccagataaaa atgcacttag atatctagta caaactatag
    40021 taatgaatga tgaattaggt gctgttattg ggaaatttag gactcgaaat aaaaataaaa
    40081 acctattaac caaatttata aatatagaaa cattaagctt tcaatggatg tcacaagctg
    40141 gtagatggca actttttaat ctatgtacaa taccaggaac taactttata ttgaggagaa
    40201 gtatcataga agaaattgga ggatgggata gtaaagcaat tgcagaagat acagaaataa
    40261 gttttaggat atataaattg ggatacaaga taaagcttgt accacaatct ataacatggg
    40321 aacaagagcc agaaactgtt aaggtgtgga taaagcaacg tactagatgg gcaaaaggga
    40381 atatatatgt attaatgaaa tatataaaaa atatatttaa acagggaaga aataagattg
    40441 tgtttgatat agcttacttt ttttctgtat attttctatt tttaacatct gtaattatat
    40501 ccgatatttt atttgtttta agtatatcta aactagtaga aatttctatt ccaattaact
    40561 tttttctaat atggatacta tcttatcttt tatttattat agaagtgagt ataagcctta
    40621 ctatagaaaa aggtgaagca acaattgaaa atatattcat agtggcaata atgtatttta
    40681 catactctca actatggctg tttgtagcta taaaaggaat gatagagtat ctaaaagata
    40741 taatttttaa aagagaggtt aaatggtata aaacagaaag attttgattc atgtacagga
    40801 ggaattaaca tgaaaaaatt tattatatca attatatctt tagtattgtt tttttcaaat
    40861 atatcactta tatacaaagt aaatgctgat gagactaaag ttaaaaatta taagtttgaa
    40921 agagatataa ctattgatgg tgttatagga agtaatagta ctttttttga agtaaataaa
    40981 aattgggata ttgaagaagt tttattgcat cttaatttta gtaagagcca aatattaaat
    41041 ggagatgttt caagtttaac tgtattgata aataatgtac caataaaatc tattaaatta
    41101 aatgctaaga caaattataa aaatacatta gaagtattag ttccaaaaga ttatataatt
    41161 caaggatata atgaaataaa aataaaaaca tacaaaacaa tatctgataa aatttgccaa
    41221 gatgattcca atactgggaa ctggatggtt attcacaaag aatcatatat atcaattaga
    41281 tataagcaga aaaaggtgga aaactcaata aatgaatatc cgtatccata tgcagaaatt
    41341 gaaaataatc ataaattaga tacaactata gtagtaccag ataatatgac tagaggagag
    41401 actacagcag tgtttaattt ggcatcagca tttggaaaaa taacaaaaaa tgatgattta
    41461 aagttagatg ttaaattata ttcagaaatg aaaaattggt cagatgataa tattatatat
    41521 attggtaaac ctgaaaatac agcagaagaa attttggata ttttaagtat aaaggaacaa
    41581 accctattat cttcaaactg tataatcaag caagtagatt caccatacaa taagaataag
    41641 aagatgatgg tggtaattgg tagtaatgaa gatgatttaa taaaggcatc aaatttatta
    41701 atagaaaata gactttctaa ccaagtttta tcatcatcag tacttgtaaa caaagagact
    41761 aatataaaaa ttaataggga acaaaaatta aatttaggtc atttaacatt aaaagattta
    41821 ggatattctg actttttgtt ggaaggagca tttaatcaac aagctttgtt tgatgtcaaa
    41881 ataccaacgg gtaaggtatt agatgatggt tcaaagatta tcttaaattt aagatattct
    41941 gacaatttag attttgaaaa atctttagtt acagtgtcta taaatgatgt tattgtggga
    42001 agtaaaaaat tggataggtc acattctaat aatgataagt tagaattaaa aatacctaaa
    42061 gatatagata ataaaaacta ttatcaagtt aaattgacat ttaatttgag tataaaaaat
    42121 tcgaattgtg ttactagaga gagtaataat ccttgggcat atgtatcaaa taattcttat
    42181 ttggcgttat ctacgaaaga aaatgaaacc ttatcttttg aaaattatcc gtatccattt
    42241 gttagagatg atgaatttaa tgatttaact gtgataatgc cagattattc agggtcacaa
    42301 gctatgacct ggatgtttag attaggtgtt actttaggtg cgaatataaa ttcacataat
    42361 ggaaatataa atgtaattag aggaaaagaa tttagtgaca agtataagga tactaatatt
    42421 gtagtttttg gagtgccaca taataattct gttataaaga tgttaaataa taatttaaat
    42481 ataaagttcg ataaaaatta ctccaatttt atatcaaatg ataagataag ctttattgat
    42541 gattatggaa aaaatatttc tactattcaa ttaataaaat caccatataa taatcaaaaa
    42601 aatatcatgg taattagttc aatgaatgaa aaaaacttat atttaggaat ggattatttg
    42661 ttgaataaaa gtaaagttaa tgatttaaaa ggagatacat tgataattga tgaatacgga
    42721 gaggtagaag atttagctta taatttaaaa tctaagaaag aagtaaaaga ttcaagttgg
    42781 aatatgagta taaataagac aactaaagta tttttaatga tttcttttat tactataatt
    42841 atagtcatga tattatcaat gctatatata aaaaaatata aaagaagata atatgataaa
    42901 tttattattt taaaagaggt atataatttg aggaaatata tgaatgaatt taaattaaaa
    42961 agaagtattt taatctcatt aataatctta atttttatat ttatagttta ctttgtaagt
    43021 attgagttaa aagataggag tctagtaaat aatcaatata aaggtaagat taaatcggcc
    43081 aatttatctg tagattatga aataaatcaa gttatgaagg atatagataa attgggattg
    43141 aatacagtaa atgtacctat aattataaat gtagagtcca ttaattctga tattatgtca
    43201 atagataata atagtaaaga aaaggcaatt aagttaataa aaaaattaaa taaaaaaggt
    43261 atttcaacaa tattagaggc atatccatgg attgaaaatg gaaaattgta tgagactgac
    43321 tggaacccta taaataaaaa aagatttttt tatacttggc aaaatgccat attaaatgaa
    43381 cttataattg atgtagcaaa tccactggat gtaaatgttt taaatattgg ttctaatttt
    43441 gtacatttgg aagaatatca acaaaattgg ggtgaaataa tagattttgt acaatccaaa
    43501 tttgacggtc ttgtaacgta cagaactaat tggtggtata caaaaaaaga tgatttaagt
    43561 agtaagttgt tttatgaaca aaagctgaac aataattttt ttgataagtt agattttata
    43621 tctatagcag catattttga gttaagtaat aaacctgtta atacagtgga tgaattaatt
    43681 tctgccttac actcatctac tgtaaacaat agaggacaaa atataaaaca agaaatatat
    43741 aacctttata aagctcatag aaaacctata ttttttggag aattaggttt ttcaaataga
    43801 gaaagtgcaa gttctcagcc gtggaatcat actccttcaa aagctgtaaa tggcgaagaa
    43861 caagcaaggt gttttgaggc atataagaaa gtttttgaaa atgaagactg gataaatggt
    43921 ttttctgtgt tttgtgtagg taaaatagat gacgaaaaaa atttttaccc atcaaaagag
    43981 agtataaaag ttattaagtc ttggtatgaa taattttgaa agtgtagaat ttatatagat
    44041 taaatagatg attaggagag atataaatga atgacatcaa ggttatgact gtatttggaa
    44101 ctagacctga agctatcaaa gtagcacctt taataaagga acttgaaaaa agagagaata
    44161 taaaaagtat tgtttgcgtt acagctcaac atagagaaat gcttgaccaa gttatagaaa
    44221 cctttaatat aaatgtagac tatgatttgg atataatgga aaaaggacaa agcctgaatg
    44281 atataacatg taagatttta aataaacttc ccttgatttt aaacaaggaa aatccaaata
    44341 taatattggt acatggagat acaaccacaa ctctagctac aagtttgact gcattttata
    44401 ataaaactct tgttggtcat atagaagctg ggcttagaac ctatgataaa tattctcctt
    44461 ttccagagga gctaaataga caacttacag gtattattgc tgatatgcac tttgctccta
    44521 ctaatttggc tagaaaaaac ttgatttctg aaggaaaacc taataataat atatttgtaa
    44581 ctggtaatac tgctattgat gctttaaaga tgacaataaa agaaaactat aatcatccta
    44641 taattgatga gattggtaac gataggatga ttttgttaac ctctcacaga agagaaaatt
    44701 tgggtaaacc tatgaaaaat atattcagag ctataaagcg aatagtagat gactttgaag
    44761 atgtacagat agtatatccc atacatttaa atcctaaaat acgaactata gctgatgaga
    44821 tatttggaaa atttcctgaa aaaattcata ttatagagcc tttagatgta gctgattttc
    44881 ataacttttt aaataaatct tatatgataa tgactgatag tggaggaata caggaagaag
    44941 ctccatcatt aggtaagcca gttcttgtgt tgcgtgataa aacagaaaga acagaaggaa
    45001 ttgaagcaaa gacacttaaa ttagtaggta ctaatgaaga tagaatatat aattcagtga
    45061 gcgatttatt aataaataaa gataactatg ttcagatgag taaagcatct aatccatatg
    45121 gagatggaaa tgcaagcaaa tatatagtgg atataataat taaaaaattt aattgcaaat
    45181 atttaaatta gataaggcct aaaacattaa ctaattgata taagatttat gataatatta
    45241 aggaatacaa tttttaaggg ggagtaatat ggctagaaaa ttaataaaaa atttgggtaa
    45301 aagtaaaagt gtaaagagag tgaaactttt atttaagaag atttttatta ctgtattcat
    45361 agtagcaagt atagttgcta tttttaatat aacgaaatat tttgaggaat tatacaaagt
    45421 aagggattta aaaagtacta aaattgaata ctatatggat gtggctgatg aagcaggaga
    45481 tggaaaggtc caattaagtt ggaaggcctt acttgctata gacatggtaa tacatgatga
    45541 agatttaagt aatataaaaa agaaagacac attggatata ggggaaaagt ttatagtaga
    45601 agataaaaat ggtaaaggcg aaaaggtgta taaggtaaaa aagtttaata aggtattaag
    45661 cgaattgaaa tttgactctt ctcaaaaaag tagagcaaga aaatacatga aagatttaga
    45721 atacacatac cttggaaata aacaattaga tagtagtgat gaaaaaatta aatttataaa
    45781 gaagttagaa gactcagcta taagagaata tattgattat ggaatattgc cctctataac
    45841 aattggacaa gctatattag aatctggttg gggaaattct aaacttacaa aacagagtaa
    45901 taatttattt ggtataaaag cagataaagc atggaaagga aaaagtgtag aaatttcaac
    45961 ttcagagcat tataatgaaa aaattgtagc tagttttagg tcgtataatt cattacaaga
    46021 ttctgtcaaa gaccacagtt tatttttaat taataataaa agatatagaa aacatgggtt
    46081 gtttgaggca aaagattata ttagtcaagc tcaagcatta gaaaatgcag gatatagtac
    46141 agcagaagat aaaaaaggaa atcgtatata tgcagagcta ttaatagatg ttattaggag
    46201 ttataatttg caattgatag ataataaagt tgagacaaaa taattgttaa ctttatatga
    46261 ggagataaca agataagatt tttttctaca aaaagaatag aaaaattgta aaaaaaacta
    46321 aaaaaatgta ttgacttgtt atttaaaagg tgatattatt attactgtac tcgaaagaga
    46381 acaaaa
//
LOCUS       DAELTA010000010        45407 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.010, whole genome shotgun sequence.
ACCESSION   DAELTA010000010 DAELTA010000000
VERSION     DAELTA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.010"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            42..881
                     /locus_tag="KPG13_000477"
     CDS             42..881
                     /locus_tag="KPG13_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0984224.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
     gene            996..1235
                     /locus_tag="KPG13_000478"
     CDS             996..1235
                     /locus_tag="KPG13_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984225.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1406..1582)
                     /locus_tag="KPG13_000479"
     CDS             complement(1406..1582)
                     /locus_tag="KPG13_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984226.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1636..1732)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2087..2761)
                     /locus_tag="KPG13_000480"
     CDS             complement(2087..2761)
                     /locus_tag="KPG13_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0984227.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(2981..3535)
                     /locus_tag="KPG13_000481"
     CDS             complement(2981..3535)
                     /locus_tag="KPG13_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0984228.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            complement(3613..3978)
                     /locus_tag="KPG13_000482"
     CDS             complement(3613..3978)
                     /locus_tag="KPG13_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984229.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4443..5228
                     /locus_tag="KPG13_000483"
     CDS             4443..5228
                     /locus_tag="KPG13_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0984230.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5325..6149)
                     /gene="pdaA"
                     /locus_tag="KPG13_000484"
     CDS             complement(5325..6149)
                     /gene="pdaA"
                     /locus_tag="KPG13_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0984231.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6370..9489
                     /locus_tag="KPG13_000485"
     CDS             6370..9489
                     /locus_tag="KPG13_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG0984232.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9507..10106
                     /locus_tag="KPG13_000486"
     CDS             9507..10106
                     /locus_tag="KPG13_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG0984233.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10333..12462)
                     /locus_tag="KPG13_000487"
     CDS             complement(10333..12462)
                     /locus_tag="KPG13_000487"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG0984234.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12728..12976
                     /locus_tag="KPG13_000488"
     CDS             12728..12976
                     /locus_tag="KPG13_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984235.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13142..14533
                     /locus_tag="KPG13_000489"
     CDS             13142..14533
                     /locus_tag="KPG13_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG0984236.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14648..16117
                     /locus_tag="KPG13_000490"
     CDS             14648..16117
                     /locus_tag="KPG13_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0984237.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16182..16775
                     /locus_tag="KPG13_000491"
     CDS             16182..16775
                     /locus_tag="KPG13_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG0984238.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            16957..18303
                     /locus_tag="KPG13_000492"
     CDS             16957..18303
                     /locus_tag="KPG13_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0984239.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18485..18856
                     /locus_tag="KPG13_000493"
     CDS             18485..18856
                     /locus_tag="KPG13_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG0984240.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18849..19538
                     /locus_tag="KPG13_000494"
     CDS             18849..19538
                     /locus_tag="KPG13_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG0984241.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20058..20924
                     /locus_tag="KPG13_000495"
     CDS             20058..20924
                     /locus_tag="KPG13_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0984242.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21166..21975
                     /locus_tag="KPG13_000496"
     CDS             21166..21975
                     /locus_tag="KPG13_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984243.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22119..23183
                     /locus_tag="KPG13_000497"
     CDS             22119..23183
                     /locus_tag="KPG13_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0984244.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23211..23930
                     /locus_tag="KPG13_000498"
     CDS             23211..23930
                     /locus_tag="KPG13_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0984245.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23954..24538
                     /locus_tag="KPG13_000499"
     CDS             23954..24538
                     /locus_tag="KPG13_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG0984246.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24540..25889
                     /gene="pabB"
                     /locus_tag="KPG13_000500"
     CDS             24540..25889
                     /gene="pabB"
                     /locus_tag="KPG13_000500"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG0984247.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25879..26622
                     /locus_tag="KPG13_000501"
     CDS             25879..26622
                     /locus_tag="KPG13_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG0984248.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26656..27357
                     /locus_tag="KPG13_000502"
     CDS             26656..27357
                     /locus_tag="KPG13_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984249.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27383..27955
                     /gene="folE"
                     /locus_tag="KPG13_000503"
     CDS             27383..27955
                     /gene="folE"
                     /locus_tag="KPG13_000503"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG0984250.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28019..28810
                     /gene="folP"
                     /locus_tag="KPG13_000504"
     CDS             28019..28810
                     /gene="folP"
                     /locus_tag="KPG13_000504"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG0984251.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28821..29186
                     /gene="folB"
                     /locus_tag="KPG13_000505"
     CDS             28821..29186
                     /gene="folB"
                     /locus_tag="KPG13_000505"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG0984252.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29179..29685
                     /gene="folK"
                     /locus_tag="KPG13_000506"
     CDS             29179..29685
                     /gene="folK"
                     /locus_tag="KPG13_000506"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG0984253.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29666..30451
                     /gene="aroF"
                     /locus_tag="KPG13_000507"
     CDS             29666..30451
                     /gene="aroF"
                     /locus_tag="KPG13_000507"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0984254.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30706..32496
                     /locus_tag="KPG13_000508"
     CDS             30706..32496
                     /locus_tag="KPG13_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG0984255.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32514..33680
                     /gene="rpoD"
                     /locus_tag="KPG13_000509"
     CDS             32514..33680
                     /gene="rpoD"
                     /locus_tag="KPG13_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG0984256.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33845..34534
                     /locus_tag="KPG13_000510"
     CDS             33845..34534
                     /locus_tag="KPG13_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0984257.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34548..35645
                     /locus_tag="KPG13_000511"
     CDS             34548..35645
                     /locus_tag="KPG13_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0984258.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35708..36280
                     /locus_tag="KPG13_000512"
     CDS             35708..36280
                     /locus_tag="KPG13_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0984259.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36340..36807)
                     /locus_tag="KPG13_000513"
     CDS             complement(36340..36807)
                     /locus_tag="KPG13_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0984260.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37193..37669)
                     /locus_tag="KPG13_000514"
     CDS             complement(37193..37669)
                     /locus_tag="KPG13_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG0984261.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37789..38880)
                     /locus_tag="KPG13_000515"
     CDS             complement(37789..38880)
                     /locus_tag="KPG13_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG0984262.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38873..39262)
                     /locus_tag="KPG13_000516"
     CDS             complement(38873..39262)
                     /locus_tag="KPG13_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984263.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39454..39900
                     /locus_tag="KPG13_000517"
     CDS             39454..39900
                     /locus_tag="KPG13_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984264.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40164..40889
                     /locus_tag="KPG13_000518"
     CDS             40164..40889
                     /locus_tag="KPG13_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984265.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40876..42120
                     /locus_tag="KPG13_000519"
     CDS             40876..42120
                     /locus_tag="KPG13_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0984266.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            42200..42877
                     /locus_tag="KPG13_000520"
     CDS             42200..42877
                     /locus_tag="KPG13_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984267.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42878..>45407
                     /locus_tag="KPG13_000521"
     CDS             42878..>45407
                     /locus_tag="KPG13_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984268.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKEN"
BASE COUNT        18433 a         4636 c         7638 g        14700 t
ORIGIN      
        1 tttaaaataa aaataagatt tcggaggagt ttattatata aatgaaagat tactataaaa
       61 taggagagat atctaaaata tatggaatag gtagagattc attaatgtat tatgaagaaa
      121 taggcattct aagaccagtt agagatataa atggatatag aatgtataat atatcagata
      181 tatggaaatt aaatttaata aaagaattta gaagcttgaa ctttccaatg aagaaaataa
      241 aagaatacct agatgacaga agtatagagt ctacgaaaaa tattttaaat gaagaactcg
      301 atttaattga taaaaaaata gctgaattta taagccataa ggaaaatatt ataaaaagat
      361 tgagttcaat tgaaagtgta attcaaaata caaaaataga tgagatagag gttgtatata
      421 tagaaaaaag aaaagcttta gaattaaatg ctgatataaa gagggatgaa gacttcgatt
      481 ttttaattca aaaacttcag aaagaatatg aagatagatt taatatactt ggaaacaaca
      541 atattggttc tgccttttct actgaagcaa ttaataaagg catatttaat gagtttaaat
      601 ctgtattttg ttttctggaa agtaatgaga aagtatataa tatagttttt gatgaaggat
      661 attatgtaac tttaaattat actgggagta attctaataa taagatgtat atggagaagg
      721 tgtttaaatt tatagaagaa aataattata agataattgc tgaccctata gaaatctata
      781 agatagatat acatgagact ggaattgtag aggagtttgt aacagaaatt caagttccta
      841 taactaaata tctatatttc aatggattag atggtaaata gaatgaaaaa atgttgattt
      901 ggcaccgatt tgccactgtg ttataaaatc ggtagcaaat tatattttaa attataagat
      961 agcatgaaaa aataattgtt tagggggtta taaatgtgaa atgttctaat tgtggaagtg
     1021 taaatattga aaagggaatt ttaacaactg gtggcatata tgctgaaact attggattaa
     1081 aatatagagg agatggaaaa ttagataaag ttataaataa aacttttcca gtacaatcta
     1141 ctatatattc agatttatgt ttagattgtg gcgaaatagt tagaacctat ataaaggata
     1201 atactgatag gaattggtgt aaagataaga aatagaataa tttttgaagt tgaattttat
     1261 taaagataca agtgaaaaag tataaatggg aagaaattgt tatatggaag aaaactttgc
     1321 atatatggaa gattagtttt ttaatataat aacattttta aatgtagata aaactaaaaa
     1381 gggttctcta gaaaacaatt tctatttaat tatacttact taccaatacc atcacttgat
     1441 gtaaacaaga ttctactaca ttttctccgt actctagtat aaaatcatct agctgtgtat
     1501 ctataaaatc acaataatca tataaactac taatataagc agttaacatg tgtgtacatt
     1561 tatccatttc atctctaaac atatatatta ctatccccct aaaaaacttt cataaaacaa
     1621 aaagaacaat atctttttgg caactggctg acataactca tagtttattt tataagcgtt
     1681 agtccctata gctttgcgtc actaaatttc tctagttttg ccgatttagt tttattctac
     1741 aactaaaata atacactagg ataagttatt gttcaacata cttgtcagaa aattgaaata
     1801 tttaacaata tcttaacaat atgaaatcta tttttcactc aagaaacaaa ctatatgtat
     1861 agaatgttta ataaaaatta tatttagttt tgtattgaag tttttgtatt aaaaagttta
     1921 gttcacaagg gttacttggc tagataatag ataaacaaaa atatcttgaa aaaatatata
     1981 tgaatggcaa aaaacaaggg atgaatttta tatagactca tcccttaatt atttatatat
     2041 tgaccttgat taaatatatt attattttta agcttcaatt tttatattat atttctaaag
     2101 tttctataag aggattatcg ctaaataatt ttaataaatt tgttttttta tatcctttag
     2161 gtaacttagt atatataact acttctaacc aatccccatc aaggcgttta atattcatgt
     2221 ttataatttc aatattagat tttttaaatt taccttcaat atattctatg gaatcagtgc
     2281 tgttagaaat tttaagagaa acttgctctg ctgtagggag tttcatccat ttcctatctc
     2341 tatgaagtgt tatctgtaca ataacaataa tgatacttga aacaatacca actaagtata
     2401 atccagagcc aatggcaaga cctgtaccgg ctgttgccca tataccagca gctgtagtaa
     2461 gaccacttac tgattgattt ctaataaata ttaatccagc acctaaaaaa cctataccac
     2521 tgacaacttg tgctgcaacg cgggctgggt ctagaccaat acctggagta cccactatat
     2581 cattaaatcc atacttggaa acaatgatta taagtgctga agctaatgcc actataaaat
     2641 gagtgcgtat tccagcttct tttagtctat tctttctctc atatccaatt aaagccccac
     2701 agaaaccagc agtaatgatt cttgctatat attcaaattg aatcaatata tgatggttca
     2761 tttatctagc acccccttct atataatatt atatatacta taatactaca ttttttatgt
     2821 gaatatagta aagtttaaaa tttatataat agttatatat gaatataaaa tgtataaacg
     2881 tatattatgt atataaaaat gatagaaagc atctaatttt aaatgattaa agagcaagct
     2941 taaataaatc aaacttgctc ttttaacata gtgtatgttt ttatttgtaa tagttagata
     3001 aaaatttatc tgtgtttttt acatctgcaa aactgttctg tagtgatgcc atgaatactt
     3061 gatgtacagt atttgcatta atttctatat tattccaaga taaattcttc gttgtacatg
     3121 catcgcttat tagagttatg tcgtatcgaa gtttctttgc tgttcttaca cttgtatcaa
     3181 tacacatatg gctcatcatt ccacagatta ctagattagt aatatttttt ttctcaagga
     3241 tactttgcaa atctgtttca aagaaactat ctggtgtgtg tttaattata atttcttcat
     3301 tatctagtgg aaaaacaatt ttatttattt ctgaaccatg agtattagga agaaaaaaag
     3361 aagctgtttt atctgttgat atatgctgta tatgaataac tggtaaattg ttttttctaa
     3421 aaaacagtag tattttttta gcattttcag cagtttcttc tgattgaaat aattcacatt
     3481 ttccattttt aaaataatca ttttgaatat caatcaatat taaagctata gacatgatta
     3541 tacctcctat ttatttttat tataatacta ttgtaaaaaa tgtaaattac ttactttttg
     3601 gtatgtatag gattacttat tgtagcaaat tcctttatta tctagaattt cttgttgacc
     3661 atgttcaagc atatattcga ttcctatttc ttgcattatt tccactgctt ttaacatttt
     3721 tttaccacga ttagtaagaa aatattcaac atgtaatgga tatccagcag aacttatttt
     3781 atcaataatc ccaaaatcac gtaactcttt taattgttga atcaacattt tttgatttat
     3841 tccataaata ttatgttgca attcagaaaa tgttttatta gattgtctta attcaaatat
     3901 aaggatagtt ttccattttc ctttgattat gtcatgtaca atttcaagag gacatgtata
     3961 ttcagctctt attttcatag catatcaccc ccagtaaaat gataataaat tatatcactt
     4021 gtcttgtgat atgacaaata tatatgaact gtatataaat tttatgtttt attaagttct
     4081 aaatttcttc tattgtagtt catatttagt acataaataa ttagtaagat ataagtaaat
     4141 aaatatataa catgactata gaaatttaac tttaaaataa cttatgataa tttagaggag
     4201 gaatatatat gaatatatca agaaaagcta tgaaaataat agaattagca caaaaaattg
     4261 caaataagag aggtatttca gtagaagaag cttggagtga agcagtaaca gaatataaaa
     4321 ataaatacga acatattgca tagatattta aaaatttaga atatttggta gaatataatg
     4381 attttaaatg atactataaa ttataaggta tatcattatt aacatagaag gaggatatct
     4441 gcttgattgt aaaaatagat aatactttag aaaaagaatt ttggcaatat gtatcacatg
     4501 aagaaagttt aaatttattc ataattggat acgttgagaa ttatggattt agctctcaat
     4561 atcaagatat atggtctcag gtagaaggtg gaaatataac ctctataata ttaaaaaata
     4621 agtcaacact tataatttat agttttaaaa ataattttaa tataggagaa atgaagaatc
     4681 atataaaaga tttagatgta gaaagcataa gtggaaaaaa atgtgtaata gatagactaa
     4741 taagtaaata caaagatttt tatgaaaaac tagataataa attttgtgta ttaaaagaaa
     4801 taaaagaaat agatttttcc aacatgaagg agtataaaat agaaaatgct caggaaaaag
     4861 atattgatga aataggaaaa cttttaaata ggtcagacta taaagtaagt aaaaattaca
     4921 tagaagaaag aaaagttcat ttaaaagagg gaaatgtaag agcatatttt attagaaatg
     4981 atgatactat gattagcact gtgtcaactg gtatggaaac tagttttttg gcaatggtgg
     5041 tctctgtaag tacagataaa agatataggg gaaaaggact tgcaagttat atggtttaca
     5101 atttgagtaa agaattgttg ttagaaggaa aagttccatg tcttttttat aataatgatg
     5161 tagcaggtaa aatatatcat aatataggat acaaagagat aaatgaatgg acaattcttt
     5221 ttaaatagct ataaaaaata aaaaactcta tcgagaatta aagtatttat aaataactat
     5281 atttttacta taattcatga tagagttttt ttaaatttat gatactattt tttattattt
     5341 aaataatcaa gtgttttaag tttataacct tctttttccc aatgtgtaag aacctcatct
     5401 aatatctcag tatttgttga tgaatttgga tgcagtaaaa ctattgcacc aggatgagtt
     5461 cttgaataaa ttttgttttt ggcaaattca tgagtaggtt gtttcttttc ataccaatct
     5521 acataagcaa aactccaaaa tattgattta tatcctaaat cttgtgtata ttttaaagat
     5581 tgttcgctaa atttacccat tggaggtcta aaatacttag gcatttcttt tccagtaaca
     5641 tctttgtatg ctttttctac acttgtaatt tcttctttga atttttcaaa atctgtaata
     5701 cctgccattg atggatggct ttttgaatga ttacaaacta aatgtccttc tttttccata
     5761 cgctttatta actctggatt gctttttata taactttcaa ctacgaaaaa ttgagcttta
     5821 gcattgtgtt ttttaagcgt atctaataat tttggagtgt ttccactttc atatccagca
     5881 tcaaatgata gatatataac tttttcattg ggatttccta cataataagc tccgtatttt
     5941 ttaaaaaaat cagcttcttt tataggtgat ggctgtttac catcttccct agggttaaaa
     6001 taccaatcat attcatgtgt gtcgagtgtt ggactagaaa tttgagtttt tgttttatct
     6061 aatgatttaa aatttatact agcaattcca aaaagtgtta gagcaaatgc tcctatcata
     6121 atgtattttt ttaggctatc ttttttcaca atttcacctc ttatttaaaa tttaaagtat
     6181 atttcttata atatagtttg aatatttata gttaaaaata ttcacatata caaataataa
     6241 atgaaagaag tttactgtaa gatataaagt tgatattaat tatcttaaat aaagtgtata
     6301 taaatgataa aatataaaga gaaatgattg taaataagtt aggctggttt aacttgaagg
     6361 gatataaaaa tggataatat aataagtgta ttgaatgatg ttgttttttt agatatagaa
     6421 gttagtggat tagattgttt aaattcagaa attttagaag ttggagctgt caaggttaaa
     6481 gactggaaaa tatataccta tgaatcatta ataaaaaaca aatttgaggt acctatagaa
     6541 gtattctcag tatgtaaaaa tttagataaa aatgatttag aaatagctaa taaaatagaa
     6601 ctggtggaag atagacttgt aaattttgta gaagatagtt tcattatatg tcatgattta
     6661 agtctgaaaa aaaagttttt tgagtatcat atgccaaagt taaaaaataa atttattgat
     6721 ttaattgaat tagcagttat tttagaacca tatcataaag attatagctt agaatactta
     6781 aaaaatactt taacaaattg taattctaaa gtggaaaata gagctttatc agatgctatt
     6841 gatataataa atattgtaaa ttgcctttta gtaaaattta ataactatga aaaaactact
     6901 ttggagccat tgagttttaa aataaattca tatcttaaaa aatttaatct tccaacatgg
     6961 gaatggagca aatttttaga agaggcaaat tatgatttat caaataatat aaatataaaa
     7021 aaagaatata atatatttga ctcaaaagaa gaaaagaaga aggaaagaga aaccttaaaa
     7081 attttaaatg aagaagaaaa aaattatgaa gaattattaa aatacaaaac tatttgggag
     7141 aataaagaag gatttacata tgaatataga cctggtcaat atgagcttac taaaacaata
     7201 agagagttgt ttagaaatag cgaagatgaa gaaaaaattg catgtataga ggcaccaact
     7261 ggtataggta aaagtgtagg atatttattg cctgcaatat tagaggctag aattaataaa
     7321 aaaagattat taatttctac tgacacaaag gaattgcaga tacaattaat aaataaagat
     7381 attccaaatg tgttagattc tttgggtcta aatggaaaag taagttacgg atatataaag
     7441 ggaaaaaata actatatttg tatagataga ctggaagcat atatagatga ttatgagtct
     7501 caaaacccta ctaaaggaga aattttatct ttaatatttt tgaagagatt agttgaaggt
     7561 ggcaagtatg gagatataga agaaataaat tatttagtgt ttgacaactt caaggaaata
     7621 agtactcact taagaaatgt aagttgtgac ccaaatatgt gcagacctaa aaaatgcaaa
     7681 aaagattgtt tatataaaaa cagaatagaa gaattaaaag aagaacatat aacagtagta
     7741 aatcattcac tattagctaa atggccgtat aaggatgaaa aacctttaga aaatataata
     7801 gtagatgaag cacataattt gactgaaaaa ggatatgatt tcttttcaag tattataaat
     7861 tcaaagtctt taaggtactt attacaagaa atttatcctt acgagtttat tcaaaatagt
     7921 agctttatat ataaaaaata tagtagaaat atgagaaaaa taaaggcttt tgataaattt
     7981 tataatgtat taaaaattgg aagagaagat aaacaaaaaa ttgctagaag tataaatttc
     8041 attattgaag agatagattc aatattaaag tttggtaatt gtaatgaata taataatgta
     8101 agtaattata atttaagatg ggaactgaat ttgcaaatag atgaaattgt gggaaaacta
     8161 aaaaaagatg gtatagatac tgaaataagt tatagagcct atagtgaaaa gataaagttg
     8221 agttgtgaga aaataataaa aaatttagtt tcaataataa taataattta taggaatatt
     8281 gatgatgata gtatagataa agaagcagat atatataaat ttggaaaggc aaagacaaga
     8341 gatttagagg atataaagat tatatttgaa atctttttag agtatgatga aaaggatgat
     8401 tatgcaagga tagttgaaat tgataagaat tataatgact ttgaatttcg agtagtacca
     8461 ttaaaaatag cagatttatt tgaagaaaat attttatctc aattagaaaa aggaatattt
     8521 ttatcagcaa ctttgagttt atctgaaagt atgtcttatt ttaaaaatac attaggaata
     8581 gacagagtta agaatgtgga aaagattata gaaccaattt ttgattataa aaatagagtc
     8641 tctgtagttg gattttctga tatttgtgag tacagaaatt ctgaatttcc aaatgagatg
     8701 agtaaaatta tatctaatat atcaaagatt acagaaggtc atacattagc tctttttaat
     8761 agtaaagata gacaagaaaa aacttatgaa atattgaaaa aatacttaca tagttttaac
     8821 ttagaaattt atgctgataa aaagggcatt agacacctta acgatttaaa tagaaaatgt
     8881 gtagtacttg gctcaaaagg atgttttgaa ggcgtagaca ttccagggga tggtttggtt
     8941 tgtgtaactt tagacaagct tccgaatcta aatcctaaag accctttgta ttttactata
     9001 atgaagaagt atggtataga ctactatact ataaattatc ctcaaatgac cataaaggta
     9061 aagcaagcta tggggagaat tttaagaagc aaatatgact atggatgttt tgtgattttt
     9121 gatgtaggaa ctaatataag tgtgttaaag agattagaga aagatttaca cgattgtaaa
     9181 atttcaaagg ttaatagcaa tgagttttat acttatataa ggcgacattt aaataaatct
     9241 agaagtttaa tactaaaatc tgttatattt gatactataa aagcgttgaa tgttgatgct
     9301 aaaatggata acaatgatgt tgataaggat attataaaaa aagatataaa tgaaaatata
     9361 agacaaagag ctgtaaaagg agaagtgtac catattgata ttataaaaaa agatatgaaa
     9421 gtaaaatatt ttgatagaaa ctatcttata aatttagata tatttatgag agaagaagat
     9481 aaaaattaag agtgggagtg atatatttgg caaaacaaaa gttttatgct gtgaaaaaag
     9541 gtaaaaatat aggtgtatat aatacatggg atgagtgcaa aaaacaagta aatggattct
     9601 caggagctga atataagagt ttttctactt ttcaagaagc taaagaatat attgatgggt
     9661 cagaaaaatt gagttttcaa gaagataagg agtttataga agcatatgta gatggaagtt
     9721 atgaacattc agtaaaaatg tatggttcgg gtgttgtgat tttaaaaaat aatgaagtta
     9781 taaaaactta tagtgaaaaa ggaaaagaga aaactttagt aagtatgaga aatgtagctg
     9841 gagagataga ggcttctaaa attgcaatgc agtactgtat tgacaataat gttcaaaact
     9901 tgattttata ctttgactat gaagggattg aaaagtggtg tactggagtt tggaaaacaa
     9961 ataaagaagg aactatagca tataaaaatt tctatgacag tattaagaat aagcttaatg
    10021 taaaatttac aaaggttaaa gctcattctg gaaataagta taatgaggaa gctgataaat
    10081 tagctaaaaa agctattggt gtataataaa aaaatattta tacataatta catggtataa
    10141 gagtatatct aaattcctac tttataatta aacaacttgt aattaaataa attttgatta
    10201 aaaaagcaga ttgtattatg aaaaaataag tatctaattt ataaacaatt aataatagta
    10261 ttaattagat acaacttatt taatataata aaatctgctt tttatataac tataaaattt
    10321 tactacattc tattagaatt gcccataaat accttcaagt tcttctttta atacttcagg
    10381 gtcatttata ccaatagaca tggttctagc cagtatattt gcttggtcat aagttgatgt
    10441 ccatgagaat acaccaccta gacaatttct aagaacatat tgagttttta agaatattga
    10501 taaagcatca tcatatgaca ttgcaaaatc tccatcctta actaaatacg gaacttgagc
    10561 agtattatca aatctatatt catatccatt tttatttata taatcccttc taagctcatc
    10621 ataagttctt gttatagtag cacctaatct tccataaaat ggtataccaa gaaggatttt
    10681 ttcagaaggc attccagcat tctcaagatt tcttaccatt gcatcaacac tatatcctgg
    10741 caaagataag tctgaatcaa aaagatttgc ttgatgtttt ctaccatttg ggcctgtttc
    10801 acctgctgta aaatcataac tcataagatt aaaataatct attataggag ctattttatc
    10861 tatttcagca cttgaattta tatatcctct atctcctgtt ccagctacac taagccattt
    10921 atcatctcct ataacatctc ttatggcagt tagtaagagt gtaaagtttt ctctatcttg
    10981 aggtcttgat gttattccag atgcactact tccaggatat tcccagtcta tatctattcc
    11041 atctaaagca tattcattta tcatctgatt gacctgtctt gcaaaattat atctagatgt
    11101 aggtgttaat gctgcatcag agaatccttc agctccccaa cctccaatag ctacaattac
    11161 ttttaagcta ggtttttcac ctttaagagc aagtaattga tttaaaaatc taggataagg
    11221 aataaacaaa tttccagact ggtctatctc agcaaaagca aatataactg catctaatag
    11281 ttgagcatct acattagttg gatttccaag aacatattct gctacaattg gatttatatc
    11341 tggacagttt gcattttcaa ctattggatt gtatgggtct gttatttgtg gtctaactaa
    11401 ttttttagaa tcttttaaag ttttcatagc tattttttta gtattttttt cgctagtata
    11461 atcgtagttt ttataattat gtttatagaa atcttcataa ctactatctt ctttgttata
    11521 gctagaattt attttgtcac aagattcatc tttatcatag ttaaaattca tttcatcaca
    11581 agacttatct ttaaacttat cttgtttgga gttgtgaact ttgttagtat ttttagtatg
    11641 ttttttctca tgattgcaat catctttaat atcagtcttg tgttcttgtt tacagtcaag
    11701 agctttgttc aggtagtcat gatgttcatg ttcacagtta cattcatttt caatgttttt
    11761 agtatgttct tttttccagc tacaagtatt atcaattttc ttgctcactt cttcatcatt
    11821 ataattatct ggtacaaaac ataagtacca gtccatacat gaatatttct tattacaact
    11881 gttaactcta ttttttaatt ccttatagtt tttaggataa ggtaaaatca ctggcattcc
    11941 aggaaaccag tttgcaggag taactatatt atctctatca ctagtctgaa gtgcatctac
    12001 tattctaagt atttctggaa tatttcttcc tgtagttagt ggataataaa gaatcgttct
    12061 tagaatttga ttattatcta taataaatac agagcgaaca gttgatgtat cactcattgg
    12121 ttttgatatc atgccgtata acttggcaat tctcatatct ctatcttcta taataggaaa
    12181 tggaatttct acacctgtaa gtaaagaaat attatacatc caagctaaat gtgaactgtt
    12241 actatcaaca cttagaccaa ttagttctgt atttcttttt ttaaattcgt cataatattt
    12301 agcaaaacat aaaaattctg tagtacaaac tggtgtaaaa tcaccaggat gtgaaaataa
    12361 aacaatccaa ttacccttat agtcagagag tctaatagga ccatttgttg tattggcttt
    12421 aaaatcagga gcctttgacc ctaaacttgg caaatttggc atgtaaatca cccctcaata
    12481 aattatcatt tattttattc tattaattaa tacatattag tgttactatt ttattaaaag
    12541 ttaagtaatc cttatgtcaa aaaaagatat ttattaaaaa gatttaatat tttcctaata
    12601 taaatgacaa tagtaattgc tattttttta ctaatttggt atagtaatgg ataaggggta
    12661 gtgtcaattt tagtgattat acgaggtgag tggattgaaa aaaattctaa gaattttagt
    12721 tatctccatg atgatatgtt ttgcaatgac ttttgcatta gtgttaataa attcagttat
    12781 gggagtacat ataggattgg attattactt tttgatggga agtgtgctta cagtttgttt
    12841 aacaattgga attgttttat gcaatgctag aaaaatattt gatagcaaag ctatacacaa
    12901 gacaagaacg aaaactgtca gtaataataa aaaacaaaag agtcattcta gggatataaa
    12961 aagaaagata tcataattta tgtaaaggaa atatttatgt ataaaataat gattgatagg
    13021 taaaataaaa acacgattta acttgtgttt ttattttttt tcaagaatta tgatataata
    13081 accaataaga aatttagttt accataaaat ataaagatta ttaaatggag agggaactat
    13141 aatgaatttt agagatttat ttgagaaggc tgtagatttt attgatgaaa aaaggaaatc
    13201 tatagttgcg atttcattat caacattagg tgtaattgct ttgataatag tatttttctt
    13261 aagtagtaac gagtttagtg taggaaatga agctaatgag ttgttaaaga ttatagagaa
    13321 aagacagtat tctatagctg ttgattacta cacaagtata gaaaaaaagt tttctgattc
    13381 aaaaatggaa aggtttaata agtctgtttc taaaaaaata aataaattgc tattaaatag
    13441 tggtgataaa tatttagatg gagacataag tcaagaaagt tttattggat taataaatac
    13501 cgtgaaagaa ttaaacaaaa taagtataga tgtggatgac ttattagctc aagctgatag
    13561 agttagagaa atgtataaag aagaaaatac tacttatgat attgccatta actacataag
    13621 tactgtttca atattaaatg gtatgggtag taacctagat gtatacaaac agaatataga
    13681 aacaatcaaa gagtctagag atgtttatga ctcagcagta aaagaccaaa aagcatataa
    13741 gtattatgaa gctattgaaa agtataataa agtattaaaa gaagatgaaa agtattacag
    13801 tatggctcaa aatgggaaag aacaatgtat tgaagaaatg tatgattact atataagtag
    13861 agctgaagag gcaaatactt ctggagatta tgaaagagca ttacaatata ttgagtattt
    13921 aaaagacgat tattcagatg atgaaaaggt acagagttta gaaaaaaaat ataaaaagaa
    13981 tttatcattg tatactttaa catctgatga cattataaat gtaatagcta aaaaaagtgg
    14041 caaagacaag gcaaatctat ctataaattc acttccacaa atgataaaaa ataataaata
    14101 ctattatgct gaagtatatg agtatgataa attaataaat gaagttttgg taagtgcaaa
    14161 aactaaagat ttatattcat ataaagatgg taaaaaagat tacaaagtag attatggtga
    14221 tggatacttt agaatattag aagatggaag ttatcaattt ggtattacaa aagataaagc
    14281 taagtttgtg ttaactaata ctcttgatga aaaagaaaat aagtataaaa agattgagat
    14341 attagatata gagaaagcag atagatatgt aaaaagtaaa aagagtttag aagaattgtt
    14401 tggaaaatat aaaaatattt attattatgc tgtagtaaat aaaggcttat ttagaggcaa
    14461 agaagtttat gctattaata tttacaatga aaaaatatat gccatatcag aaaatggact
    14521 aaatgaatat taattaataa tattaaatta atatataatt aaaaagtttt tggggtttta
    14581 aggcgcatta tgtagtaata taataatagg gatatataaa taattttatt gtaaggagaa
    14641 gtatgggatg agtaatgtga acaaaaagct agttatagct attggagctg tagcattagt
    14701 agtaataatt tttataggca taatgtgcat gcaatttaaa ggggagaaaa tagcaaaaaa
    14761 cacatatgtt aatggagtaa atattggaaa attaactaaa tcacaagcaa aacaggaatt
    14821 agctaaaaaa tacaaattag agaatgttga ttttaattac aatgataaaa gctggaaagt
    14881 aaagagcaaa gatttaaatt tatctactga cctggataaa acagtagaaa acgcatataa
    14941 tttaaataga aagagtgctt tctttggcaa tctatcaaaa actatttcag caaattttgg
    15001 caaaaaaagc aacttagtag ttgcgataaa ttatgataaa aataaattaa aagcagaaat
    15061 ggaaaagata gctaaagaaa tagatgtaga tgtaaaagat gctacacttg atataagtgg
    15121 tgaaaaagtc aaggttatac cagattctga tggtcttaaa atggatattt ctaagagtat
    15181 ggagaatttt gataatcaaa ctaaaaaagg taactataaa aatgagttag tagtaaaagc
    15241 tactccagca aaagtaaaaa aagagcaact tgctaatata gatacaaatt taggtacgta
    15301 ttcaacaaca tttaaaactt ctcaaataaa tagaagtata aacataaaat tagcaacaga
    15361 taatataagt aatgtattac ttatgccagg agaaactttc tcatttaata aacatacagg
    15421 gaaaagaagt aaggaaaatg gatataaaag tgcacctgta ataatggaag gtgagatgga
    15481 agaagattat ggtggaggag tttgtcaagt ttcatctacg ttatacaatt ctgtgctata
    15541 cgcaggttta gaaattgtta atgtaaaaaa tcatactata ccatcaagtt atgttccaaa
    15601 aggaagagat gcaactgttg cagatagtgg aatagatttc ttatttaaaa ataatctaaa
    15661 gcatccagtt tacataaaaa attacgtttc aggaaatcaa attgtttgca atatatatgg
    15721 aagtgcagaa gataaacaaa atataacaat atcaacaaaa ttagatggcg tttcacaaac
    15781 tacaatgaaa agagttaatg accctacaat gcctaaaggt aaagaaaaag tagacaaaag
    15841 tggaagaaat gcatattctg tatcaacata tagaacattt aatgatgcaa atggtaaaaa
    15901 aattaaaact gaaaaaatag ctaattcata ttatcctaag aaggaaggta ttatattagt
    15961 tgggactatg gaaccaaaac cagaggaaaa accaaataca gacgaaaata agaataatca
    16021 aaatacaaac aatcaaaacc caaataatca acaaaaacca gaaacaccac caactgataa
    16081 taagcctaat gagacacaac cacaaccgca agcttaaagt tctcaagaat aggatttatg
    16141 tagagtaata aaaaatataa atctaaatag gagggattac tttgaattta aataatataa
    16201 cgaattacgt tcaaagtatt tgcgagaata tttctaatgt cttggatgta gatgttactt
    16261 tagttacaaa agatttaact aggatagcag gtacaggaat attcagaggt aaaataggag
    16321 agacaatatc agagaagtca gtgtactctc ttgttttaaa agatgggaaa tcctatgtta
    16381 taaataaaga aatagaagag aactgtaata aatgtgcaaa tagagaaaat tgtaaagagt
    16441 tagctgatat atgtactccc gttaaaatgg gagagcatat acttggaatc ttgggtatag
    16501 ctgcgtttaa tgaagaacaa aaagaaaaga tactttcaaa agataaggag ttaactgctt
    16561 ttgtaaggag tatgtctgat ttaatatctt ttaaactaga tgaactttat aaacaagaag
    16621 ttaagactat agatgacctt gagagggaag ctatagaaaa tgccataaag aaatatggaa
    16681 gtaatacaga aggtatgaaa aaagtagctg aggcattaaa tataggtata gctacactct
    16741 atagaaaagt aaaaaaattc aacattaaaa gttaatagta ttgaaaaaga ggtaaccttt
    16801 ttatgaaaag aagaggatta ccttttttat tatgagaatg gtggaaaaat atgtatagtt
    16861 ttgacttgac atattaaata tgttatctta aatatatatt ttagagtgga ggtgagtatt
    16921 attttagtta aagatataaa acaagacata gaaagcatgc ctagaaaaat tttagatata
    16981 tctttgatag aagataataa aagtgatttg acttttttta atatagaaga agaagacggt
    17041 gatgaaacat taaaagcatt aaaacagagt tatgtgacta caaattataa agaagaaact
    17101 ctattaaact tgcaaggagc tttaaattca aagtcagata caacgtctaa ggagttaaaa
    17161 ttagaatcat ataaaagata taacaatgca ttggatttag catataaaaa ttatataact
    17221 agtgccatta atatggttaa taaagctctg gagataaatc ctaaagacat tgatatacta
    17281 aatcttagag gtcttttaaa attattaaaa tgtgattttg ctaaatcatt tgagagtttt
    17341 tatactgctt tatgttatga aaataatcat atatctagaa aatatgtaaa tctaatttca
    17401 tctgaagatt ttaaaacttt tttaggaaga tacaatcatt ctataagatt tataaatgaa
    17461 gaattaaatt atgagtcaat acatatttta gagaacataa ttgaagaaga acctgaacta
    17521 atagagccat atgtaatttt atctttgtta tatgataaac taggtaatgt aaaaaagaga
    17581 gaaggatatt tagacaaact taaagagcta gataaagata atcctgtatt tgaaaaaaat
    17641 gaagaagaaa aagaagatac aagtaaaaag gaagaaataa aaaaagtaaa aagaaagaaa
    17701 agtattttgc catatattat agttggctgt gttttaatag gtatggggat atatttgatt
    17761 caaagcaaaa agagaataga aaacttgaat aaccaaataa gtagtaaaga ggaaaagtta
    17821 agtgaaactg ataaaaagtt aggagaaacg agtaaaaaat tagaaaaaac gaataaggaa
    17881 ttaaatgagg ttaaaaataa agaaccagaa agagagataa ctgtagctga tgaggaagat
    17941 ttatattatc aagctctaaa tttaaagaaa aacaaagaat atgaaaaagc catagataat
    18001 tttaaaagtg tagtttcttc tggtaaaact aaaaaatata tttctgagtc aatatatcaa
    18061 ttagcaataa caaataaatt attaggaaat aaggatgaag ctataaaata ttacaaaaaa
    18121 tatataaata cttatactaa aaatgaccag tattatgatg attcatatta cgagttgggt
    18181 atgctttatt atgataatgg cgatttaaaa aatgcacaac agacttttta tagtttgaga
    18241 agtgaagttc cagacagtat gtataacaat tcaaaaataa aagaaatttt aagtgaaaaa
    18301 taggtttata tagttaagtt ttttgaaaca accaagtatg tattttatta aattaaaata
    18361 aaaataaatt taaaaagaga ttgtgtcaaa ttataacttt ctattttaaa gaaagatatt
    18421 tgcttgttga taaaactaca aatatatgat ttgaagcaat ctctttaatt tttggaggtg
    18481 gaaaatgaaa gttttcaatc aattggtaat aattttatct atctgggtaa taggagagta
    18541 tataagttcc tttatacaag gaattatact tattcctggg agtatagttg gtatgctatt
    18601 attatttcta ctactacaat ttaaagtttt agacttgagt agtatagaaa atgtaagtgg
    18661 atttttcctc gataatatgg ctatattttt tattccagca ggtgtatcac ttataaagtc
    18721 attagaccta ataagggaaa atgtattagt gcttttatta gtcatatgtt taagtacact
    18781 aatagtaatg tatactactg gaattattgt ggagaaaatg ataaaaaaga aggaagaagg
    18841 acaaaaaaat gtataatgta ttacagacac cagtatttgg tataatagta acaatagttt
    18901 ttttcaattt aggaagatac attcaacgaa aaacaagtaa tcctatatgt aaccctttat
    18961 taattgcaat cgtaggaata atattgtttt taagcatatc taaaatccct tatgagaatt
    19021 acaagatagg agctgatagt ctaaatttct ttttaggtcc tgtcacagta gtacttgcag
    19081 ttccacttta taaacaattt gaattattta aaaagcatat gtttgagatt attgtaggaa
    19141 taggttgtgg tatagtgatt tcttttgtat ctgtattgat aattggcaaa atagctaatt
    19201 cagatgtaag tataataaat tcactaattc caaaatctat aacaacacca atgggaatat
    19261 ctttagcaaa ttctttaaat ggtgtagaat caataacagt tgtagccata atttttacag
    19321 gtatttttgg tggtatggtt gcttctacag tattcaaact ggggaatata aatcatccag
    19381 ttgctaaagg aattgctctt ggaacatctg cacatgcttt aggaactaca aaggcttttg
    19441 aacttggaga aatagaagga gctatgagtg gtgtttctat tggagtctct ggtacaatta
    19501 cagtaatact aattccaatt ataatgaatt ttatataaaa aataaaatta agacgagtaa
    19561 cttgaatact taaaacaaaa taaaataaaa aaaatttaaa ataaaaactg aacgaaaaaa
    19621 atgcaaataa tgaaataaaa aaatcatgta ctgaaattat aaaaagaaaa atttcattta
    19681 ggaattaatt ataaagagac aattactaaa cttgaatttg ctaaaataaa ataaattact
    19741 gcgaaaatag atatttagaa aaaaatacta taaatttact atattgcaag ttaaataata
    19801 agcagtatca tattatgcta gaaaaagttt catttaaggc ataaatgaga tgatttgaac
    19861 gtttttaaag attagaatat aaaagattgc actagacata tatgaaaaag ataatataat
    19921 agatgatgta atagatgaca gttcaagaaa ttataaaaaa aatttatttg aaatattatc
    19981 tattacatca ggaataatat atgtaagagt aagcttttaa aattctaaag atttagaaag
    20041 attgaaggga gaaagaaatg agtgaagtta agaataccct acaaaaacat ccagttttaa
    20101 gatattcaac aatgctattt gggatgttga tgacatctat aggaataaat gggttcttaa
    20161 gaccagcaca tctacttagt ggaggtgcta caggtatagc aacatctata aactatttga
    20221 caaatataaa tgtaggttta ttaacatttt taatcaatat tccaatattt atcttagggt
    20281 tcatatacct tgaaaaagag ttttgtattt caagtttggt aaatatgatt gtattttcat
    20341 tattattagg agcaactcag gaaataagta atattatacc tatacatgat atattgcttc
    20401 aaagtgtata tggtggtata ttatctggtt taggagtcgg agttgtattt agaactaggt
    20461 cttctcaagg tggtacagat ataatagctg caatactaaa aattaagaag aatatagaaa
    20521 tgaaagacac tgctttagca ataaatggct tgatagtact tacaggaagt tttttatttg
    20581 ggttggattt agcattatac actcttatag gtctatttct aaatgcgtat tcaatgagtt
    20641 ttattaaaga tgcaatgaat taccaaaaat ctgttatggt aatgtcaaat gaagtcgatt
    20701 taatagcaga agatataatg aagagtttag ttagaggtgt aactttttta gatgcagaag
    20761 gtgcatacac tcatcaaaaa aagaagatta tatatacaat agtttcatca aatgagatac
    20821 caaagataaa agatatagct ttgaagtatg ataaaaaggc ctttatatct gtaaatgatg
    20881 ttacagaagt aaaaggaaga gggtttaaag caaaagattt atagaaaatt ttacaagctg
    20941 ggagtttata atgtaaattt gtatattata tctctcagct tatttaaatt tagtatacga
    21001 aaataaaggt tttattaatg atttgtgtat cactttaaaa gatatcattt taaaattaaa
    21061 attgaaaaaa ttacttaata tgtagaaaac atgaccacta ttaggatata ataaatttaa
    21121 tagatttagt aaaatatcta ggaataacgt tggaggaatg gcattatgaa tttatctaaa
    21181 gaatatgtta atttgtatga taattatttg aaatatataa atgaagtaaa caaagagatt
    21241 agaagtataa agaacatgaa gaaacgtgtt ttaaaaaggg gcgattttgc tccagaaaat
    21301 gaagtattag aattggaagg aaaaacaata cgcgatatag atgatgtcga tacaaaaaaa
    21361 ccaaaaaata aaatctataa attttcaaat ggggatgtat atgtagggaa attcttagag
    21421 ggaaagatgg aggggaaagg ctcatatact ttttttgtag atgaaggaac agttatggag
    21481 tacataggag aatttaaaga agataaaaaa aatggaaaag gaaactttat attttcaaat
    21541 ggaaatgaat atataggtta ttttgaggaa gatatgatga gcggtattgg aaatatgtta
    21601 tatagtagta aagatgaata tattggaact tggaaaaacg gaaaaaaaga tggaaaaggt
    21661 atttataagt ggagtgatgg atgtatatat gcaggtgaat ttaaaggtgg aaagatggaa
    21721 gggtacggta tttgctataa tagtaaaggg gaagtacttt atgaaggaga atggaagaat
    21781 aatttgattc atggtaaggg aacttatata tgggaaaaag gtaaaaagta tataggtgaa
    21841 tttatgcatg gtaaaaaaca tgggcaagga actttttatc taaataatga attggtttat
    21901 gaagggactt ggaaatttga taagccatct atatttgata gaacattaga tgaaatattt
    21961 tccttgagac tgtagcttta tctaattagt agataaagct tttttctaat acttactgta
    22021 aataggtatg aatgatattg attagtatac actatcaata ttaacttaat aaatgcaata
    22081 ttaaaaaatt ttgattgatt ttaagaagga gagatatatt gaagagatta attgctttag
    22141 tgttagtagt acttattgtt ttattttata gtcctgaatt attaaaagaa ggatatgatt
    22201 taaaagaaaa gttttttcaa agtgaaatat ggaatgaatt tttagacaaa ataaatataa
    22261 atgataattc taagaaagat aaaaaagaaa aaagtcaaaa tttacaatca aataatactg
    22321 aaggggagaa cttaggagag tcagatataa agaattttga aaaaataagc ttaggagata
    22381 atttaagcta tgttttatca agtatcggca aacctggtag aattgatatt agtgaatatg
    22441 gttttgattg gtatgtatat aatcaatatg gaaaagaatt tgcaatggtt ggacttgaaa
    22501 atgatgaagt agtagcactt tatagtaatt ctattaattc atgtgaaaat caagatataa
    22561 aactaaatca agatagacaa acagtgagaa ctaagattac accattaaaa tataagagaa
    22621 aaggaaatac cagatatata ataaactcag aaaatcaata tgatataata agtaaggaag
    22681 gtaagtatat tacaattttc tacgatattc atgaagaaaa tagagtttgt tcatatttaa
    22741 taattgataa aagcactgaa gatgagttta aaaatttata tccagatgat agcgaagaac
    22801 ttaaaaaatg ttttgaactt gaagttatag accttgtaaa tagtgttaga aatcaaagag
    22861 ggttaaactc attgagatac agtgaacagg caactttgag ttcaagaaaa catagtgaag
    22921 atatgagaga taataatttt tttgaccatg taaataagaa gaatgaaact ccatttgaca
    22981 gaatgaaaag agaaggtata gtatatactt cagctggaga aaacatagca gcaggacaga
    23041 ttaatgcaat atatgcgcat gaagcttgga tgaactctga aggacataga aaaaatattt
    23101 tggggaacta taataatatt ggagttggag taatatttgg aggaagctat aaaacatatt
    23161 acactcaaaa tttttataaa taactaaaat atttgaaagg aagttaaaaa atgacatcat
    23221 ggaaaaaaaa gacagtatat aagtgcttaa tagcagtagt cttgttttgt ggaatagtgt
    23281 taatatctaa ttttagtaaa gtttctgctt tgatgatgga tactaatgga aatgttttga
    23341 ttaaacatgg ttcaagggaa aaaaagctaa tagcaattac ctttgatgat ggtcctcatc
    23401 ctaaagaaac tagtcaagtt ttagatgtat taaagaaata caatgtaaaa gctacttttt
    23461 ttatagctgg aaaacatgca aaatggtata aagaaccatt agtaagagca agtaaagaag
    23521 gtcacgagat aggaaatcat actttcaatc atcctgatat aagtaattta agttctagtc
    23581 agatagaaga ggagattgtt aagtgtgaag acattttgaa agaagtaact ggaaaaaaac
    23641 cgacattatt tagaccacca tttggaagtt atagagaaaa agaattaata gaaattgcaa
    23701 aaaagcatga ctataaagtt gttttatgga caggtgtaga tgtgaaagat tggaaaaatc
    23761 caggagctaa tagcattgca gataaaataa ttaataaagt acaaaatgga gatataatac
    23821 ttttgcatga ttatgcaact aatgatacag ttgaagcttt agatatgttt attccaaaaa
    23881 tgattgaaaa aggttttaaa tttgtaacag tttctgagtt aataaaataa tttttagggg
    23941 ggagatttac agtatgattc ttatgataga caattatgat tcttttgtat acaacctagt
    24001 ccaatatata gaagaattag gggaaacagt tgtagtaaaa agaaataatg aaatcaaaat
    24061 aagtgacatt gaagaactaa atccagaggt aattgtgtta tctccaggac cgtgttctcc
    24121 aaaagaagct ggtatttgca tagatattgt tgagcatttt aaggggaaaa aacctatact
    24181 aggaatttgt ttaggtcacc aaactattgg acatgttttt ggaggagata ttataaaagc
    24241 acaacagcct gtgcatggaa aagtatatag tatcaaccat actaataaag gagtttttag
    24301 gggacttaaa aatcctttaa atgtcactag gtatcattca ctaatagttg actccaatac
    24361 agttccaaaa gaattagaaa ttacagcaat tactgataaa ggggaaataa tggggattag
    24421 gcataagaaa tatttaatag aaggagtaca gtttcatcca gaagctattt tgtcagagta
    24481 tggtcatgaa atgcttaaaa attttataac agaagctaga gaaagggtac atatgtaata
    24541 tgataagaga gataaatact aaattaaatt cttttgagat atttactatt tttagaaatg
    24601 aacatgacag ttttatatta gacagtgcta tggacaaaga aaaattggga agatattctt
    24661 ttataagtag ccaaccattt aaggtattaa agtataaaga tactgatgaa aatcctttag
    24721 aagttttaaa agaagaacta cataagtata gggttgtaaa tgatactaat cttccatttg
    24781 taggaggtgc tgtgggatat ttatcttatg atttaggtaa ttacatagaa aatttaccta
    24841 gaactgctgt agatgatatt gagatgcctg atatgtattt tggattttat aatcatgtaa
    24901 tagttataga ccatcttgtg caaaaaactt atatagctac tccaaacata gatatcgaat
    24961 tagaagaaaa gataatagat gatatagaac aaagaatatt aaaagaagag aaaaaaggta
    25021 tagatagcat atgttatgaa gaaaaagaag taacacctat aagacttaaa tctaatttta
    25081 caaaggaaga atttaaaaat gcagttcaaa gtgttagaga gtacataaga caaggagata
    25141 tatatcaagc taatttaaca caaaggttca gtggagaaac agaacttaca agctttgaat
    25201 tgtataggga cttaagaaga tttagcccag caccatttgg ggcattctta aactttgaag
    25261 atgcacatat attatcaaat tctccagaaa gatttataag atgtgttaat aagagaatag
    25321 aaactagacc aataaaaggt actcgtccaa gagggaaaga taaagaagaa gatttaagac
    25381 ttcaacaaga acttagaaat agtgaaaaag atagagctga actacttatg atagtagatt
    25441 tagaaagaaa tgatattggg agaatatcta aaactggaag tgttaaagtt ccagagctat
    25501 ttgtaattga accatatgca aatgtaaatc acttagtttc cacagttgta ggtgagttaa
    25561 aagatgataa agatgctact gatgtaataa aagcaacttt tccagggggg tctataacag
    25621 gagcacctaa gattagagca atggaaatta tagacgaatt agagccaacg caaagaaatg
    25681 tatatactgg ttcaataggt tatataggtt ttaatggtga tatggatttt aatatagcaa
    25741 ttagaacaat aatcaaaaat gacaaaaaag tctattttca agttggagga ggaatgactt
    25801 gggattctga cccagatgaa gaatatcaag aaacacttga taaagctaaa tccataatga
    25861 aggctttgag gggatattat gaagaataat gtaggttttg atagtagttt aagtaaattt
    25921 ggaattggtt tgtttgaaac tattagagtt aaaaatggaa tagcagtaga tttagatatc
    25981 catatagaaa gaatgctaag ttcaataaat tgtttggatt tagatataaa atataaaaaa
    26041 gatttcttga taaatgaaat tgttacatat ataaaaaaag aaaatgtcat taataaagca
    26101 cttagaatta ctgtttttga tgaaggttat aatatatcta ttagggatat tccatataat
    26161 caagagacgt atgagaaggg gtttaaatta gctatatctc caatagttag ggggaatagt
    26221 ttaattcata gacataagac tactaattat tttgaaaaca tatataccaa aaatatcgca
    26281 aataagactg gttataatga tggtatattt ttaaattcag aaggtgtaat attagaatgt
    26341 tctatgagca atattttctt tgtaaaagga gaaagagtgt acactcctag tgatagatta
    26401 ccgatattaa atggaataat aaaaaaaagg attatagaga tttgtgacga attacatata
    26461 gagttgatag aaaatgaaat taatatatca gaaattagta gttttgattt tgtttttgtc
    26521 acaaatagtc ttatgggcgc tataaaggtt acagaaattg acaaaataaa atttaataaa
    26581 gaaaatgttg tttttgataa aatagtgaag ttattagaat aaattaaaat attaacaatt
    26641 tgttggagac aaaagatgat aggttgtatt tatgaagtat acaatgaaga gataactaaa
    26701 ataatagaaa accaaaacac tttggctgta tatttagtgg gttctagtaa agacgtagat
    26761 tttactcttt atgatgtaga gataaacgat attgatgtgt tcgtttttgt aaaagaaggg
    26821 gaaaaacaag agagaatact gtttaaaaaa aagggaatag aatttgatgt aaattacttt
    26881 tcaaaagatg gtgtaaaaaa attattatca aatagggaat atttctttgt gaaagaaatg
    26941 aaagatgcta aagtactgtt tgatagggag aatatatcac atattataaa agatataagt
    27001 agaaatattt acttagaagg acccagtaaa atgtcattag aggaaaagtc ttttataaag
    27061 caagatattg gagcaaaaat atcgaattta aaaaataaag aaaaatttga tgtttttgaa
    27121 tatcattttt tgacgaattt atatttaaaa gatataataa ttgggtactt taatataaat
    27181 gataaatggg taccaaaaga caaaaaattg ctaaaagttt taaaaaaaga aaataatgag
    27241 ctttttgaac tagtgtcaaa agtaagtgaa aattatgact atcaaagact tttagatgtt
    27301 tataattata tttttaaaga aattgagaca aaagaggtta taaaactaat cttctagtca
    27361 caaatcaatg gggggagttt aaatgaataa agttgataaa gaaaagatac aacatgcagt
    27421 aagagaaata ctagaggcaa ttggggaaga cccagataga gaaggtctaa tcgaaactcc
    27481 aaatagagtt gctaggatgt atgaagagat attctctggt cttagtgaag aaccaagaga
    27541 ccatttaaaa gttctttttg cagatgaaaa acatgaagag ttagtattgg taaaagatat
    27601 tccattttat tcatgttgtg agcatcatct agttcctttc tttggaaaag ctcatattgc
    27661 ttatttacca aaaggaggaa gacttacagg gctttctaaa cttgctagag taatagatac
    27721 attggcaaaa agaccacaat tacaagaaag aatcactaaa aatgctgcag atataataat
    27781 ggaagaatta caaccttatg gtgtcttagt agtagtagaa gctgaacata tgtgcatgac
    27841 aatgagaggc gtaaaaaaac caggttcaaa aactgttaca tcagcagtga gaggaatatt
    27901 tgaaaaggac attgcgtcta gagccgaggc tatgagtttg attactatga agtagcaatt
    27961 tattatagat ataatcattt attgagataa aatttaattt aagggagtgt cattaattgt
    28021 gtttgattat ggtaaaagaa cttatataat gggaatatta aatgtaactc cagatagttt
    28081 ttctgatgga ggagatttta ataacttgga catagcaata caacatgcaa aagatatggt
    28141 tgaccaaggt gcagatataa tagacctagg aggagagtct acacgcccag gtcatagcta
    28201 tgtagactca gacgaagagc taagaagagt aatccctgta ataaaaaaac ttaaacagga
    28261 attggatata ccaatatcaa tagacactta taaggcagat gttgcagaag aagcattaaa
    28321 attaggtgtc actatggtaa atgatgtttg gggtcttaga aaagataaaa atatggcttc
    28381 tgtaatagga aaatatgatg cagaagtgtg tataatgcat aatcaagatg ggacaaacta
    28441 tgacaaagat ataatggagt caataaaaga cttttttaag gtaagtatag aaatggcaat
    28501 gagttgtggt gtaaaaaaag aaaaaatagt acttgaccca ggtgtaggat ttggcaagga
    28561 ttttgaacaa aatattgaag tattgagaag attaaatgaa ttaaaagatt tagggtatcc
    28621 tattttgctt gggacatcca gaaaatcagt aataggaaaa gtacttccag ttgagcctaa
    28681 aaaaagatta gaaggtacaa tagctactac tgttcttggt ataagagatg gcgtagatat
    28741 agttagagta catgatgttt atgaaaattt aatggcagct agaatgactg atgcaatata
    28801 tagaaagtag gtataatata atggataaaa tattacttag taatctagga ttctatggat
    28861 atcatggtgt tcttaaagaa gaaaattttc taggtcagaa attctttgtc gacatggaac
    28921 tttatattga ttcaagagaa gcaggtctta gtgacgatat aaataaatct gtaagctatg
    28981 ccgaggttta caatgttgtt aaagatatta ctgaaaataa acaatttaat cttttggagg
    29041 ctttagcgga aaatattgct gaagaagtct tgaataaatt tatacttata aatggagtta
    29101 tggtcagagt tagaaagcct gaggctcctg taaatggtat ttatgactat tttggtgttg
    29161 agataaggag aacaagagat gaataaggct tatttgggaa taggtactaa catgggtgat
    29221 agatttgata atttatcaag agcatgtgaa cttttgaaaa atagtgattc tatatataaa
    29281 gtaaaagaat caagcttata cgaaacaaaa ccatggggat atacagaaca agcagatttt
    29341 ctaaatatgt gtgtagaaat agaaacagag tttgaacctt atgagttgtt agaatattgt
    29401 caagaaatag agagagaact acacagagag agaatagttc attggggacc aagaactata
    29461 gatgtagacg tactattttt taatgatgta gtttccactg atgaaagact aacaatacct
    29521 catccaagga ttcaagatag agctttcgtt ttgataccac ttatggattt aaatgaagaa
    29581 cttataataa atgaaaaaac aataaaagaa catttaaacc tcctatctgc tgaagaaaga
    29641 gaagaagtaa aggagcttgt aggttatgaa agaaaaccta tttaataata attcaaagat
    29701 agagataaat acaaataaca aaaagataac tgtaaaaaaa gataaactta taatagctgg
    29761 accatgcgca atagagagtt atgagcaact tttggagact gctaaatttg taaaaagtca
    29821 aggagcaaac attttaagag gtggagcata caagccaaga acatcgccaa attcatttca
    29881 aggattaaaa aaagaaggtc ttgaaatatt aaaagcagtt aaagacgaag ttggtatggc
    29941 tgttattaca gaacttatgg atgtaagaga tatggacgaa ctgtatagta taagtgatat
    30001 aattcaaatt gggtcaagaa atatgcaaaa ctttactctt ttaagtgaag tgggaaaaca
    30061 aaataaacct gttatgttaa aaagaggaat agcatccaca attactgaat ggataggtgc
    30121 ttctgaatat atagcaatag aaggaaatag taatattata atgtgtgaga gaggtataag
    30181 aacgtataat gattatacta gaaatacatt agatttagct gcggtaccca ttattcaaaa
    30241 ggaaacaggt cttcctgttg tggtagaccc aagccatgcc actggagtta gatatttggt
    30301 aaaacctatg tcactggcat cttttgcatg tggagcagat ggaataatgg tagaagtaca
    30361 tcctgaccca gaaaatgctt tgtcagatgg tgctcaatct ctttgcttta atgaatttga
    30421 agatttaatg aaatcaatta ataattatta aaagaggcaa gagcctcttt tttattcgta
    30481 atcgttgcta aggtaaattt ataagttata atttgatagt tagattattt aatataaata
    30541 aagatttaat attgatagaa ttaatataaa aaataaaaat tttttgatta ataagaagga
    30601 tatcaataaa aaaagtcgaa ttgtgtatat tgagtgattg acaaatttag aatatagtgt
    30661 agaatagaaa tgttaacaca aaaaattaca ccagaaggtg ataatatgaa tgatataaaa
    30721 gacattattg aagaaataaa agctagatgc gatatagcaa gtataatatc tgaatatatg
    30781 aatataaaac aatctggagc taattacaaa ggattatgtc catttcatgg cgaaaagaca
    30841 ccttcttttt atataaatac atcaaaacag atttataagt gttttggttg tggtgaaggt
    30901 ggagacgtaa taaattttgt catgaaaatg gaaaatttgg attttatgga tgctgtaaaa
    30961 attttagcaa ataaatgcgg tatagaaatt aacactaaca tgaatgaaga aactaggatt
    31021 aaaatagaaa aatctaagaa atttcaagat attcatacgg aagcagctag attttatctt
    31081 tcaaacttat tgggaagcaa aaatcttggg tatgaatatt taagaataag aggactagat
    31141 gataaaatta ttaagaaatt tggtttagga ttttctttgg attcatggaa ttcacttatg
    31201 aatactctaa tcagtaaagg atataaaaaa caagacttat tagagtgcgg tctaattgca
    31261 aaaaatagag atggaactaa ttgctatgat aaatttagaa atagagttat gtttcctata
    31321 ttcgattata gaggaaatat aataggattt gggggaagag tattagacga ttctttgcca
    31381 aaatacttaa attctccaga cacattaatt tttaataaaa aacagaactt atacggatta
    31441 aattttgcta gaaaaaattt agaaagtaaa actatagtct tagttgaagg atatatggat
    31501 ttaatttcac tttatcagta tggtataaaa aatgtagtag caacccttgg aacagccttg
    31561 actgaacaac aaggcatttt aataaagaga tatgcggaca ctgctattat atcttatgat
    31621 tctgatgaag caggaataaa agcgacttta agagctatag atatacttac taaattaggt
    31681 atcaatgtaa aagtgttaga cttaaaagat gctaaagacc cagatgaatt tgtaagaaaa
    31741 tacggactta gtgattataa aaaagctatg gatgtttcta ctcactacat aaaatataaa
    31801 atagatcatc ttaaaaaaga atttaacatt caaaaagatg aagaacgagt gaagttcgca
    31861 aaagaagctt ctaaaattat aaaacaattg acaagtcctg ttgaaattga tttttataca
    31921 aagtatttaa gcaatcaaat agatattaat gtcgagtcta taaagagaga agtttatggt
    31981 aaaaattaca ataagccata taataacaaa aatcaaaaga aaatagaaga aaaagtaatt
    32041 gaaaaagtcg aagtcagaca agacggaaaa caattagttg aagaaactct aataaaaata
    32101 atgttagaag acaaaaaaat tagagaaata gcgttactta aagtagaaga aagtgatttt
    32161 ttattgaaag aaagtaaaga aattttaaat tacatgatta aaaatcaaga attggacaaa
    32221 ataactattg acaaattgaa aagtttaaac atatccgaag aatacttaaa agaattaaat
    32281 tcaatttctt tgaatagtat aaacttagag aacacaaaag aaatagaagg aataataaca
    32341 aatatcagaa aaaactcatt agaagagcaa attaatagct tgttgagaga acaacaagaa
    32401 ttagaaaata ataatgatat gaaagaggta gatggtagag ttatggaaat tgctttaaaa
    32461 atagttgaga taaataaaat tttaaagagc ttgtagagaa aggaggtttg agtgtggaaa
    32521 ataaatcgaa taaaaaagag ttaaaaaagg ttactgctaa gacattaata gaaaaaggaa
    32581 aaaagcaagg ttctctgaca cttgcagaga taatggaagc tttttcggag actgaacttg
    32641 ataaggatca agtagaaaat ctttatgaga ctttaggaaa tttgggaata gaaataacag
    32701 aaacaaaaaa ctataaagct gatatagatt tttcggttgc tgatgacgat ttaagtatag
    32761 gccacttaga tgaagatgca gaggcaattt cacatgatga ctcttctgca atagaaatag
    32821 aaactgtgga tttatcttta ccaaaaggga taagtataga tgaccctgta agaatgtact
    32881 taaaagaaat aggaaaaatt cctctactta aaccacatga agaagtagaa tttgctagaa
    32941 ggatgcacga aggtgatgag atagcaaaac aaaggttagt tgaagcaaac ttaagactag
    33001 ttgtaagtat agcaaaaaga tatgtaggaa gaggtatgct tttcttggat ttaatacaag
    33061 agggaaattt aggtcttata aaagcagtcg aaaaatttga ctatacaaaa ggatataagt
    33121 ttagtactta tgcaacatgg tggataagac aagctataac acgtgctatt gcagaccaag
    33181 ctagaactat aagaatacca gttcatatgg tagagactat aaataagcta ataagagtat
    33241 caagacaact acttcaagaa cttggaagag atccaaaacc agaagaaatt gcaaaagaaa
    33301 tggaaatgac agaagataaa gtaagagaaa taatgaaaat agctcaagac cctgtgtctt
    33361 tagaaacacc aataggagaa gaagaagata gccatttagg tgactttatt ccagatgatg
    33421 atgctccagc cccagcagag gcagcagcat attctttatt aaaagaacaa atagaagatg
    33481 tacttggttc attaaatgat agagaacaaa aagtattaaa gcttagattt ggtcttgagg
    33541 atggtagagc cagaactctt gaggaagttg gaaaagagtt tgatgtaact agagagagaa
    33601 taagacagat agaagcaaaa gcactaagaa aactaagaca tccaagtaga tcaaaaaaac
    33661 ttagagatta tttagactaa aaagatacat cattgatgtt tttgtgataa atgccatgag
    33721 ataagtgata tctcatggct attttgtagt aaagatttaa ttttattttt gtagttattt
    33781 cagtctaata tctataactt ggattaaatc ctataaataa agggaattga aaggagaatt
    33841 atatttgaaa ttaacagata gattattgaa aatagcatca ttagtgtcag atggtaaaaa
    33901 aatagctgat ataggaacgg accacggata cataccagta tatttgttaa aagagggaaa
    33961 agtaccattt gcagtgctag cggatgttaa taaaggacct cttgacaatg cacataaaga
    34021 agtcatacag aacaatcttt tagataaagt tgatttacga ttgggttctg gtatagagat
    34081 acttgaaata ggggaagtag aagaagtgat aattgctggt atgggtggaa tcttgataag
    34141 tgagcttctc gaagctaaaa aagaagtagc acataatgta gaaaaattaa tattacagcc
    34201 aatgcaagca caagaagaac ttagatatta ccttttaaac aacggatatg aaattttaga
    34261 agaagtttta gtgagagaag attttagaat atatgagata attgttgcca aatatacagg
    34321 taaaaatact ataatagaag atgagatata ttatgaggtt ggaataaagc ttttagaaaa
    34381 caaagattct ctttttaatg attttataga aaaaaagata aagacatata gttctatagt
    34441 taataaatta gaagggaaaa atggggaagc tatagataag aagagaaagg aaagcgaagt
    34501 tgcaataaaa aaacttgaaa atttaataaa ataaatagga ggtatatatg ctattaaaga
    34561 gtctaactag aaaaattgaa aaaaagtatc ctctaaactt agcagaggat tgggataatg
    34621 taggtcttat agttggagat tttgatatgg atgtaaaaaa ggtattggta tctttagaag
    34681 ctaatgaaga tgttattgat gaagctatat ccaaaaatat agatttaatt gtgacacatc
    34741 atccttttat atttgggaaa attaataaaa ttaatagtgg agatttaaaa ggcagactta
    34801 tccaaaaact tattaaaaat gatatatcac tttattctat gcatacgaat tttgatatag
    34861 catttgatgg tctaaatgac tattttatgg aaattatgga atttggtaat tcaaaggttt
    34921 tagatataac taaaagcgaa aatttgtata aattagcagt atacgttcca cataactatt
    34981 cagatgaact tagaaaggtt ttaagtaatt caggagcagg tcatataggg aattatagtg
    35041 attgtacgtt ttcaacagaa ggtgaaggac aatttaagcc attagaaggc tctaatccgt
    35101 ttcttggaag tgtaaatgat atagaaactg taaatgaagt taaaattgaa actgtagtac
    35161 cacagaagct tttaggtggg gtaataagtt ctatgttaga tgcacatcct tatgaagaag
    35221 ttgcatatga tttgtacaag ttagaaaata aaggtgagat atttggacta ggtagaataa
    35281 gtaaacttga taaaagtatg acattagaaa gtctttcaag gaaaataaaa gagaaattaa
    35341 atatgaaaca cataagagta gttggaaacc ttagtacaga cataacgaaa gtagccgttg
    35401 taacaggagc tggttcagaa tttgtaaaaa aagctaagag acaaggtgca gaagtactaa
    35461 ttacgggtga tgtaaagtat catgaggcac aagatgcact tgatattgga atgtgtatag
    35521 tggattgtgg tcattttgat acagaagata tattcaaaaa tgttatgaag agatttttag
    35581 atgaatttag tgaaattgaa gtaataaaga gtaatgtata tttgaatcca tttagtacta
    35641 tttagcagtg tttgataata aaaatactat ataaaaaata aatttgagtt taattagggg
    35701 gataatgatg aaagtagcag taatttttca tagtgtttgt gggagtacat atttattagc
    35761 tagagaatat aaagaagcgc ttgaagaaat gaatatcgag gtagatatat ttagagtaag
    35821 tgacgaagtt gcaaaaacac ttcctcaata ttatttgata aattccaaag aatacaaaga
    35881 cgaatttgaa agtataaatg taattaaatc tggaaaagaa atattagatt atgatgctat
    35941 atttatgggt tcaccaacat attatggaaa tgtatctgga ccaatgaaga tgtttatgga
    36001 tagtttttct gatatttggg taggtgctcc tttgagtggt aaaatatttg gttgttttgc
    36061 tacagcaggt tcacaacatg gaggaggaga attagctttg caagctatga atatatttgc
    36121 ccaacatatg ggaatgactc ttttgtcagt tccatgttca gtaagaggag gttatccagc
    36181 gtatggaata cttcatatag caggagataa ttctgatata aggccaaatg atgatgccaa
    36241 gataggtata agagattacc taaaaagact taacatataa gaatatcata taaaaatacc
    36301 taaaagcgac tgtatatact tttaggtatt tttacttaat tataaacgtg aagctttacc
    36361 agaaatatgg tcaatttcta ttttaacaac agttgttaag ttcatatcct tctctatgta
    36421 cttcatgcct tcttcaaaga atcctttaga atattttttt attatttcta caagagcatt
    36481 tttcttttct tcattttcta cagtgcaagc ttttccaaaa actatagcac tagaataagt
    36541 agtactaaat ttatctggaa taacactttc attagcaact actaaaaaag aaactttgtt
    36601 gtttttcgat atattatcta acttatgccc atttcttgca caatgaaagt aaatagaatc
    36661 attaaagtat acataattta cagcaactcc ataaggataa ccattttcag atatagtgga
    36721 aaaagtacca aattcaccat tttttaatac ttcaacagta tcttcttttg tcatttctct
    36781 ttttttcaat ctcatttctt taaacataat atcatccttt ctaattttga tgcttaaatt
    36841 aaattttaga acatttataa agattatgct atgtttttta caaattttaa gaaaaaaata
    36901 tagtttaaga taaaaggtag tgaaagagaa gctatttgga ggtttattta tgatattatt
    36961 gttagagtag tacaaataat actaataaat ttaatagctg gtttcttgtt atttaaggta
    37021 gtaagcatgt ctataagaag gggaagttat cgagggtaga agttgttgtt tataacgata
    37081 ttataaagaa aatattatta tataaaataa taaatagtaa agaggtatca agttgaatat
    37141 aatacttaat acctcttcaa aattaagtta taaaagtgtt gtgggaattg tcctatacat
    37201 ggtaaggaga taataaagaa tgtatcttta aagtctcaac taaacagcaa aatttatcca
    37261 acaaacatac aataaatgct tctttgtatc taggtggctt aacagtaagt ggccacatgt
    37321 gttttaaaat tatgtccgtt tctttttcat ttatttgaaa aaataaagtt gcatttttta
    37381 atgcttctct agggtgagta aatccatgta atccttttct atcacctttg taatgccaat
    37441 cataaagaaa aaagtcgtgt agtaaagctc ctctaataac actttgaata tcaacactta
    37501 agtgcaattt tttacataat agataactat aatatgctac tgacaaacta tgttcaagac
    37561 atgaaacatt gccatgatga ggaaatgttc tcataagttg aactttttca gaagaaagta
    37621 tctcagaagc acattcatta aaatatttga tttcagcttt tgacaacatt atgaaaatct
    37681 ccattcttag taatatataa atactatact tcaacgataa tatgaaatat ttcttatcct
    37741 acaaaatata atacccaata atacaagtaa gatacctttt atggtatttt aaagtaagag
    37801 agattttatt aggctaaaca atgaaaaaat taaaagtaaa gctatagcaa tatatagtat
    37861 ttttccaaga ggattgccaa aatttatagt ccatccaata ccaaaccttt tattaaccat
    37921 taatgatggg tcatttggat tattatacat gatgcctgct atccaatact tctcatcttc
    37981 ttctggagaa taagatgagt tgaattttaa atttggggaa ttaatatatg ttgctattaa
    38041 ataaattata actaaaagcg ttgtaatatt catagcagtt aatagttttg tttttattga
    38101 taaaagagta gtaaaaaact gtgttgtcat tataatcatc aatattaaaa acaaataacc
    38161 aattttattt aaatatttaa tgttttcaac tctactttct tcaattctat ttgtatctat
    38221 cttaatcctt gactttatgg atccaatgga agttatatat aataaaacca ttaaacaaaa
    38281 gtcagtaccg attaatttaa atacattaag aaatgattta tctataaaaa catcaggaga
    38341 cccatttatg ttccaatgag tggcaattga gtctggcaat tgattataat taaatgctat
    38401 aaaaatactc attcccaaag taaatagtat cggtattaaa tagattattt taaatttttt
    38461 tattatttta tttttcttat ttataaaatc catatctact attaattttg aatctatatc
    38521 tatatgacct aatttagcat aaatttctga tttaagcttc ttagcttttt tatgtgtatg
    38581 aatatatagt atactttcat ataataaaaa acctataata ccaataaaat aggaaagttc
    38641 caatttatta aatgtaaaga ccagaataaa aactaatata aagtctataa taaaacctaa
    38701 agataatagt tttttaaatt ctttatctaa ctttataaag taattataat ttttatgctc
    38761 ttggtctata gaaactccat agatgtgttt tttacctaca aaatagggta taaaataaaa
    38821 tgtagcatac atcaaaatta gaactggaaa tattgtaaat agacattcaa tcttactcat
    38881 aatatttcac ctcatacttg tcaaataaac ttgaacttag tgacaaaaat tcttctttac
    38941 taatacctga taaataagat tttgccacca ataattccaa gtcaagttta tttttttgac
    39001 gagttgtatt atctattttc attggtagtt gagctacaat agcacctttt cttctatcaa
    39061 tatttaagaa tccctcttct tttaattcat tgtaggattt gttcactgta tgtaaattta
    39121 caccaatatt ttcagccata cttcttacag atggaagtga ttcatttatt ttcaattctc
    39181 ctgaagctat agccttagtt atttcttctt taatttgtac ataaatagat ttatcactat
    39241 taaaatctaa atttaaaacc ataaaaaacc tccattatgc tttataaatg aaaacgattg
    39301 ttatatacat agtataacat aaaaaataat aacaaacaca aaatataagt ttatattttg
    39361 taaaaattgt aatttaaaaa ataacataat ttttattaaa ataatacaga tactatttat
    39421 agatagaaat aaaaattaga aaggagaaaa aatatgttaa catatttaat gaaaagacaa
    39481 atgcataaaa aagatgatgg aatgagtaaa ccaatgatga ttacaggagc tgtaattaca
    39541 ggtgtaggag catatgctgt atataaaatg gtaaaaagcc ataaatctaa ttctcaaatg
    39601 gtcaatacaa attattatgg aaatagcgat tatgatgatt ataaatatga cgaatttgat
    39661 tatgactgtg attgtagaga cacaaaacaa gattatgaat atgaagaatt aaaaaaaaaa
    39721 gtaaaagaat ttaattcaag aagaataaat tgtgaaaatt gtccacatgt ttctcaagaa
    39781 gaaatgatgc aatatgtaaa tagtgttaaa gatgtaaagg aagagcttga tttacatgaa
    39841 gatgctaaaa aagataatat ttacaaagaa gatgaataca aaaaatatga agaagaataa
    39901 gtaatatttt gtataactga acaaatagta atgtcaaatt atttaatatc tagttttaat
    39961 atgtaagttg taacttaact tgaaagaatc agtatcattc tttagatagt taagtaacaa
    40021 cttacatttt atttttataa attttaatag ttctttgtga ctattttgta attttagaaa
    40081 tattttagta tccagggtaa ttgtgtataa taaaacttgt atagatagat agtatgaaat
    40141 taagcagata gtttaaaatt aaaatgaaaa gaaaggaaat agttatgtta aagttgttga
    40201 tagttgaaga tgatagtact atatcttttg gaataaagta tgctttagaa caagaaggat
    40261 ttagtataga tatatcaaag gatttatcta gtggaaaaga aatgatttct tctaatgaat
    40321 atagcatgat actgttggat gttacattgc cagatggaac tggttatgag ctttgtcaat
    40381 atataagagg attctctcag gttcctataa tattcttgac tgcttgtgat gaagaagtaa
    40441 atattgtaat gggtctcgat ataggtggag atgattatat aacaaaacct tttagaatta
    40501 gagaattaat atccagaata aaagctgttt taagaagaaa aggtaatact tcagaagaaa
    40561 ataagaaaat acttaaattt ggagatttaa gtatatatac attagaagct agagtatata
    40621 aaaatgataa agaaatattt ctgacatcag ttgaatataa acttctttta attcttatac
    40681 agaataaaaa tactgtttta agtagaactc aaatattgga aaaactttgg gatgtaactt
    40741 atgattttgt aaacgacaat actctaactg tttatataaa aaggcttaga gaaaaaatag
    40801 gtgatgattt atgtgaacca atctacatag aaactgtaag ggggattggt tataaatgga
    40861 taggaagtga aaatagtgtt tctatataat ccagaagtga agaagttttt aagtaagtat
    40921 gttactttga tgtttattgt tatcataata tctattggat ttagcataat caacgtgtcc
    40981 ttaactaaag agatggttgt aagaaataat caggctataa taggaacttt atcaagcaag
    41041 tatccaaact tagaaagtca gatagtagac ataataactc aagggaagtc tatggagaac
    41101 atagattatg gtcagaaaat attaattaag tataattatg ataaatctat taggataaac
    41161 tctgagccta ttatttctaa attagtttta gagactatca aaataaatat aatattagta
    41221 tgtataatat ttattttgat atttgtgttg attgttagat attttacgtc aatttacaat
    41281 gatttatcag atatgactaa gtatgtttac tatagttcag agggtaagcc atttgatatg
    41341 aaaaacaaga atcaagaagg gcagataggt cttctaaaaa ctgaactctt aaaaatgacg
    41401 acaattctta atgaaaaggt tgaactttta aaaagagaaa agatattttt aaacaataca
    41461 atttccgata tatcacatca attaaaaact cctatgactt ctttaataat gttgaatgat
    41521 ttattatata atgatgtacc atacgaagta aaaatcgatt ttttaaataa gataaaaaat
    41581 caattaaata gaatggattg gttgattaaa agcatgctta aattatcaaa ggtagaagca
    41641 aaagtgataa attttaaaaa agataaggta aaatttagtg aactcataca tagagcaatg
    41701 ctatctatga aaatacctat ggagattaaa aatcaaaaac tgactataga gggaaatgat
    41761 aatgtatctt atattggtga tattgattgg tccgtggaag cattagtgaa tataatcaaa
    41821 aattgtgtag aacatactcc tgaatttgga aatataatta taacctataa agaaaatcca
    41881 ttattttctg aactaataat aaaagatgag ggtgaaggta tagataaaaa agatatacca
    41941 catgtattta aacgattcta tagaggaaga agtagttcaa aagaagatag tgtaggaata
    42001 ggtcttgcaa tgtcaaaatc tataatagaa agccaaaatg gcgatatata tgtaaacagc
    42061 gaaaaaggta agggtacaga atttcatata atatttcata aaatgtatga tagtgactaa
    42121 tctgtaatat ttaattcact ttatagtaat agccaaaacc taatatatga agtatagaat
    42181 ataaatattg gaggtagtta tggaaatttt aagagtagaa aatctaacta aaagttatgg
    42241 taaaaatgaa acaaaagttg atgctataaa aaatgtaagt ttatcagttg aaaaaggaac
    42301 gtttatagca ataacaggtc caagtggaag tggtaaaagt accttattac atttattagg
    42361 aggagttgac agaccaacta gtggtaaggt ttatataaat gatgttgata tatataattt
    42421 aaaaactaag gatttggcga tatttagaag aagaaatata ggtcttatat atcaatttta
    42481 caacttaatt ccagtcttaa atgtgaagga aaacatattg cttccagcag agcttgacaa
    42541 tagagatatt gatagagaat attttgatga tttaatgaaa acattaggtc taattgatag
    42601 agaaaagcac cttccaaacc aagtatcagg aggtcaacaa cagagaacct ctataggacg
    42661 tgcattaatt aatagacctt ctataatatt agcagatgag ccaacaggta atttagatag
    42721 taaaaattca aaggaaatat tagaattgtt aaaattgtca gtaaaaaaat acaaccagac
    42781 actaataatg ataactcatg acaaaaatat ggcacttcaa gctgatagaa ttataagcat
    42841 agaagatgga atcataaaaa gtgatgaggt gatgtagatg aatttatata catcactaac
    42901 tattagatac ttaaaacaaa ataaaagaag aaccattgta accataatcg gtataatact
    42961 ttcaacagct ttaatttgtg gtataggaaa tatatttgaa agttttatgg attatcaaat
    43021 gagagaaact ataaagaatg atggaagttt ccatgttaca ttttatgatg taaatagaaa
    43081 aaatgtggaa tatgtaacga aatcagctga aatagaaaaa catgcattta ccaaacaact
    43141 tggatattct aaattagaaa atagtgaaaa tgctatatta agtataaagc aatatgataa
    43201 aaatgcaata aatggatata aagtttctat aaaagaaggt agatttccag ctaaggaagg
    43261 agaaattgtt ttaagtgaaa gtattattaa tcttatagat gataagttaa aaataggaga
    43321 taagataact ttaaaagtag gggatatgtt tgactcaagc aagaaaaaga ttgatagtat
    43381 gacatttcat gaaggtgatt atatagcaaa tgaaaaagat agaactttta aagttgttgg
    43441 tataattgaa aaacctgggt ttgaacgtta taatggaatt gcaacagcta tagcctactt
    43501 taatcctatg aataattaca atgatacaat taatgtatca gtagcagtaa aaaatcctaa
    43561 agatgtgtat aagataagta ataaaataag taaaaatata tattcaaata aagataaaga
    43621 agcagtttct gatatgataa aatacaatga acatttattg aggttacaag gtgctagtaa
    43681 gtattcaaat ataaatagct ctataaagtc tataattata gcagtaacta ttttagtaat
    43741 cgtatgtaca attgctacag tgtacaattc ctttagtatt tctataaatg aacgaaaaaa
    43801 acagtttggg atattaaatt ctataggtgc tacatctagt caaattaaaa gattagtttt
    43861 tatagaaggt attataatta gtttaattgg tataccaata ggactaatat ctggaacagt
    43921 agctatagac ttattgttta aaattataaa taagtatttt actgaatcgg ttgttactaa
    43981 gatgagtttg cagatagtgt ataatccaat aataattatt gttagtataa taatagtttt
    44041 gcttacaatc tttatatcaa ttctactacc tgcaatatcg gcttctaata tttctcccct
    44101 taatgtgatt aaaaatactg gagagtataa agtgggcaaa atcaaaagct caagacttat
    44161 taaaatgatt tttaaaactg aaggagtact tgcatataaa aatattagga gaaatagaaa
    44221 aaaatttata ataactcttt tttctttaat ggtaagtata ataatattta tatcttttag
    44281 tggctttaca ttacttttac taagaggtga agaaattaga aattctcaaa gaaaatatga
    44341 tttgtattta actgcaaaga acacagctag gtctgtagag gatactataa gtgaactaga
    44401 agatattgat gggataaaaa actttgaatt ggcaacagga caatatgtgt ctataagagt
    44461 aagtgaaaat aaaataaata aatccaagga agatttaatc aggaaatatt atcaaaaata
    44521 taagattgga gatagttatg aatatgattt cagtaataat gaattaattt ttccaggaga
    44581 ttttgcagta aaaaacaaaa taaataactt agtacagggc tcttttaata aagagagagc
    44641 aattgaagaa aatggtgtta tattagttag aaaaagtgca attgaagaaa atgggaagaa
    44701 aggtgtagta gaacttacca actataaagt aggagataca gtaaattgtg aatatcttga
    44761 tgaaaatgcc ttaagtaaaa aagtaaaggt caagattctt gctataactg acgaagaacg
    44821 tctaggtctt ggttatcaaa atatggggtt acaatttata acttatgatg aagttgctaa
    44881 aaatttaaat ttaaaattaa gtaaaagcct tatatttatt gattcaggag gaagcattaa
    44941 gactaagaag gaaatagaag ctttggcaaa taaaaataat tttaactttt atgatgaaag
    45001 tgcaagcaat gaaagtgaaa aacaagattt aaaggttatt aaaatatttg tatatggttt
    45061 tatagtagtg atatctttag tgagtgttac aaatattcta aatacagtaa gtacaagtat
    45121 aaatctaaga aaaagagaac ttgcagtaat acaatctata ggggttactc ctaaaggatt
    45181 taggaaaatg atatatttag aaagttttat atatggtata ctgtcattgt tgtttggaat
    45241 accaattagt attggtataa ttctaattat gaataaactt atttcaggtg taattgaatt
    45301 ttctccgatt ataccgtgga cagccatagt tatctgtata gtaagtgttt ttataataac
    45361 ttttatagca gggtatatac caatgagtaa attaaataaa gaaaata
//
LOCUS       DAELTA010000011        45313 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.011, whole genome shotgun sequence.
ACCESSION   DAELTA010000011 DAELTA010000000
VERSION     DAELTA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.011"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            141..356
                     /locus_tag="KPG13_000522"
     CDS             141..356
                     /locus_tag="KPG13_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984269.1"
                     /translation="MKISDFNKNQIGTAVQLQDIRFGTKVDCIITKVEKNEILVMYYE
                     KETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            385..1116
                     /locus_tag="KPG13_000523"
     CDS             385..1116
                     /locus_tag="KPG13_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0984270.1"
                     /translation="MDLLKDARKKEWFWLENDLVDREDLGIYEKMIYIVLARYSDNES
                     CCFPSYKTIALKCGCSKRQAKSVVKILENKGLIKKENRIKSNSNEKESNIYFVLTAKL
                     GGEYDAQQVVNMMHNPSAPHAQQVVHEVHSKKTYNKKTYIKSNTTTQKESKTDYLDLS
                     FLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALLTWG
                     NGDASKGIVPEVTKTKNPLSGFKEL"
     gene            1144..1329
                     /locus_tag="KPG13_000524"
     CDS             1144..1329
                     /locus_tag="KPG13_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984271.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            1355..1573
                     /locus_tag="KPG13_000525"
     CDS             1355..1573
                     /locus_tag="KPG13_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984272.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(1592..1804)
                     /locus_tag="KPG13_000526"
     CDS             complement(1592..1804)
                     /locus_tag="KPG13_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984273.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            1967..2227
                     /locus_tag="KPG13_000527"
     CDS             1967..2227
                     /locus_tag="KPG13_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984274.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(2220..2408)
                     /locus_tag="KPG13_000528"
     CDS             complement(2220..2408)
                     /locus_tag="KPG13_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984275.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            2546..2863
                     /locus_tag="KPG13_000529"
     CDS             2546..2863
                     /locus_tag="KPG13_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984276.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            2885..4216
                     /locus_tag="KPG13_000530"
     CDS             2885..4216
                     /locus_tag="KPG13_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0984277.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            4591..4977
                     /locus_tag="KPG13_000531"
     CDS             4591..4977
                     /locus_tag="KPG13_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984278.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            5030..5734
                     /locus_tag="KPG13_000532"
     CDS             5030..5734
                     /locus_tag="KPG13_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984279.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            5791..5973
                     /locus_tag="KPG13_000533"
     CDS             5791..5973
                     /locus_tag="KPG13_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984280.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            5970..6347
                     /gene="ssb"
                     /locus_tag="KPG13_000534"
     CDS             5970..6347
                     /gene="ssb"
                     /locus_tag="KPG13_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0984281.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            6328..6912
                     /locus_tag="KPG13_000535"
     CDS             6328..6912
                     /locus_tag="KPG13_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0984282.1"
                     /translation="MKIFHFKLDGDYVRSLEERNKLVEENINFVWFIIRRYFKVFISK
                     YPYLQDDTFSEGCIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRAD
                     IDSFEKCIYKNTHGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLREK
                     GYTQKEIGKILRTNQMTICRKLGKLKSEINILGK"
     gene            6942..7100
                     /locus_tag="KPG13_000536"
     CDS             6942..7100
                     /locus_tag="KPG13_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984283.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            7103..7429
                     /locus_tag="KPG13_000537"
     CDS             7103..7429
                     /locus_tag="KPG13_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984284.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            7429..7965
                     /locus_tag="KPG13_000538"
     CDS             7429..7965
                     /locus_tag="KPG13_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBG0984285.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            7977..8783
                     /locus_tag="KPG13_000539"
     CDS             7977..8783
                     /locus_tag="KPG13_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984286.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            8808..9068
                     /locus_tag="KPG13_000540"
     CDS             8808..9068
                     /locus_tag="KPG13_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984287.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            9092..9577
                     /locus_tag="KPG13_000541"
     CDS             9092..9577
                     /locus_tag="KPG13_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984288.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            9597..9854
                     /locus_tag="KPG13_000542"
     CDS             9597..9854
                     /locus_tag="KPG13_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984289.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            9963..10406
                     /locus_tag="KPG13_000543"
     CDS             9963..10406
                     /locus_tag="KPG13_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HBG0984290.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            10549..11094
                     /locus_tag="KPG13_000544"
     CDS             10549..11094
                     /locus_tag="KPG13_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984291.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            11098..11742
                     /locus_tag="KPG13_000545"
     CDS             11098..11742
                     /locus_tag="KPG13_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0984292.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            11957..12415
                     /locus_tag="KPG13_000546"
     CDS             11957..12415
                     /locus_tag="KPG13_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0984293.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            12970..14709
                     /locus_tag="KPG13_000547"
     CDS             12970..14709
                     /locus_tag="KPG13_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984294.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFHILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            14710..14913
                     /locus_tag="KPG13_000548"
     CDS             14710..14913
                     /locus_tag="KPG13_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984295.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            14926..16203
                     /locus_tag="KPG13_000549"
     CDS             14926..16203
                     /locus_tag="KPG13_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0984296.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            16181..16942
                     /locus_tag="KPG13_000550"
     CDS             16181..16942
                     /locus_tag="KPG13_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0984297.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            17001..17327
                     /locus_tag="KPG13_000551"
     CDS             17001..17327
                     /locus_tag="KPG13_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984298.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            17353..17955
                     /locus_tag="KPG13_000552"
     CDS             17353..17955
                     /locus_tag="KPG13_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBG0984299.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            17984..18907
                     /locus_tag="KPG13_000553"
     CDS             17984..18907
                     /locus_tag="KPG13_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984300.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            18921..19193
                     /locus_tag="KPG13_000554"
     CDS             18921..19193
                     /locus_tag="KPG13_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984301.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            19177..19557
                     /locus_tag="KPG13_000555"
     CDS             19177..19557
                     /locus_tag="KPG13_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBG0984302.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     TALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            19569..19916
                     /locus_tag="KPG13_000556"
     CDS             19569..19916
                     /locus_tag="KPG13_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984303.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            19916..20272
                     /locus_tag="KPG13_000557"
     CDS             19916..20272
                     /locus_tag="KPG13_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0984304.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            20281..20730
                     /locus_tag="KPG13_000558"
     CDS             20281..20730
                     /locus_tag="KPG13_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984305.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            20731..21786
                     /locus_tag="KPG13_000559"
     CDS             20731..21786
                     /locus_tag="KPG13_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0984306.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            21802..22242
                     /locus_tag="KPG13_000560"
     CDS             21802..22242
                     /locus_tag="KPG13_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0984307.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            22257..22673
                     /locus_tag="KPG13_000561"
     CDS             22257..22673
                     /locus_tag="KPG13_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0984308.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            22700..22849
                     /locus_tag="KPG13_000562"
     CDS             22700..22849
                     /locus_tag="KPG13_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984309.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            22853..26242
                     /locus_tag="KPG13_000563"
     CDS             22853..26242
                     /locus_tag="KPG13_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBG0984310.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            26289..26765
                     /locus_tag="KPG13_000564"
     CDS             26289..26765
                     /locus_tag="KPG13_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBG0984311.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            26823..27458
                     /locus_tag="KPG13_000565"
     CDS             26823..27458
                     /locus_tag="KPG13_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0984312.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            27699..29216
                     /locus_tag="KPG13_000566"
     CDS             27699..29216
                     /locus_tag="KPG13_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0984313.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            29230..29562
                     /locus_tag="KPG13_000567"
     CDS             29230..29562
                     /locus_tag="KPG13_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0984314.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            29555..29989
                     /locus_tag="KPG13_000568"
     CDS             29555..29989
                     /locus_tag="KPG13_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0984315.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            29982..31034
                     /locus_tag="KPG13_000569"
     CDS             29982..31034
                     /locus_tag="KPG13_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0984316.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            31036..31644
                     /locus_tag="KPG13_000570"
     CDS             31036..31644
                     /locus_tag="KPG13_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0984317.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            31661..32443
                     /locus_tag="KPG13_000571"
     CDS             31661..32443
                     /locus_tag="KPG13_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0984318.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            33039..33527
                     /locus_tag="KPG13_000572"
                     /pseudo
     CDS             33039..33527
                     /locus_tag="KPG13_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33929..34276
                     /locus_tag="KPG13_000573"
     CDS             33929..34276
                     /locus_tag="KPG13_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984319.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            34288..34458
                     /locus_tag="KPG13_000574"
     CDS             34288..34458
                     /locus_tag="KPG13_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984320.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            34488..34769
                     /locus_tag="KPG13_000575"
                     /pseudo
     CDS             34488..34769
                     /locus_tag="KPG13_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35077..35544
                     /locus_tag="KPG13_000576"
     CDS             35077..35544
                     /locus_tag="KPG13_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBG0984321.1"
                     /translation="MSKSYFNTKLGGSMNVTIVFLATNIFIKLVILAIAFDTLLGCLR
                     AIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLG
                     ISEFFSILFILYESVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            35548..36378
                     /locus_tag="KPG13_000577"
     CDS             35548..36378
                     /locus_tag="KPG13_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0984322.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            36450..37556
                     /locus_tag="KPG13_000578"
     CDS             36450..37556
                     /locus_tag="KPG13_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBG0984323.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            37694..38029
                     /locus_tag="KPG13_000579"
                     /pseudo
     CDS             37694..38029
                     /locus_tag="KPG13_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(38040..38555)
                     /locus_tag="KPG13_000580"
     CDS             complement(38040..38555)
                     /locus_tag="KPG13_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984324.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(38638..39543)
                     /locus_tag="KPG13_000581"
     CDS             complement(38638..39543)
                     /locus_tag="KPG13_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBG0984325.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(39563..39766)
                     /locus_tag="KPG13_000582"
     CDS             complement(39563..39766)
                     /locus_tag="KPG13_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984326.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(40015..40473)
                     /locus_tag="KPG13_000583"
     CDS             complement(40015..40473)
                     /locus_tag="KPG13_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984327.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(40508..40810)
                     /locus_tag="KPG13_000584"
     CDS             complement(40508..40810)
                     /locus_tag="KPG13_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984328.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(41198..41350)
                     /locus_tag="KPG13_000585"
     CDS             complement(41198..41350)
                     /locus_tag="KPG13_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984329.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(41532..42689)
                     /locus_tag="KPG13_000586"
     CDS             complement(41532..42689)
                     /locus_tag="KPG13_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBG0984330.1"
                     /translation="MNQVKDQKDNILMKSNKFIMARYDLQTIENRLFVRLLYEFQKNK
                     NLEITITIEEVRELMKNPNYTTPQAISKIFTKLRTGTIYIKDDDSWGECSFIYHWIYN
                     EKDKTYTVKTSAELLDLIENYIQVGYTPINLQIFFSLRNSYAQRFYDLLRLWTRTKNK
                     ITYTLDVLKELLLLEDKYSKYNDLKRRVIVPAIQELNSTEYFDIDFKEIKKSRKVVAI
                     EFSVKDLDKRKYFDKNVVETVIYEEQEESKETETTIEFYIPDETAFTKGTIRLFKIDF
                     KEFDFKKDIYIQAFDKSLAIALERDNADIINTKNYDFFKSTLSNKISEVIIQEKDDLK
                     FKEELDKYWDDEDVKKEEREIDYSKDPVKLETVRRNLFDQGLVNEEGYYYK"
     gene            complement(43572..43955)
                     /locus_tag="KPG13_000587"
     CDS             complement(43572..43955)
                     /locus_tag="KPG13_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0984331.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHRNSFESFISAL
                     HDDEEISANKLKSLEKFIEDWEEDK"
     gene            44540..44695
                     /locus_tag="KPG13_000588"
     CDS             44540..44695
                     /locus_tag="KPG13_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984332.1"
                     /translation="MGELKLESKMNCTKNKKKVAISIHQMKKISKKDYVFLTDCIETF
                     YMKIKEV"
     gene            complement(44721..45089)
                     /locus_tag="KPG13_000589"
     CDS             complement(44721..45089)
                     /locus_tag="KPG13_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984333.1"
                     /translation="MAEVSKKLKAKEIGKRLNELRTLERLSQRAFGERIFLSQDQISL
                     LEKGKRVLTERSINDICREFEVNEEWLRSGKGEIYKDCLADLEIDDDVKEITNKLYEL
                     EQEDRDAILKMIELLQKKNK"
BASE COUNT        18218 a         5244 c         7619 g        14232 t
ORIGIN      
        1 aaaaaaaaac gaaccaaatg aatggagaac tatagaaacc ataatctcca gcacatacgc
       61 attaagtaat ttcaaaaaag aaaataaaga cattatgaat cagaaataac aacagtaaat
      121 acttaaaggg agtggtaagc ttgaaaattt cagattttaa taaaaatcaa ataggaacag
      181 cagttcaatt acaagatata aggtttggta caaaagttga ttgcatcatt acgaaagtag
      241 aaaagaatga aatactagtc atgtattatg aaaaagagac agaggaaata gcatataaaa
      301 ctttgactaa agaagatctg atacttgatg attataaact taagttatta tcttaaaaaa
      361 taatttcgga ggggaggtga tgagatggat ctcttgaagg atgcaagaaa aaaagagtgg
      421 ttttggctag agaatgatct agtagataga gaagatttag gaatctatga aaaaatgatt
      481 tatatagttc ttgcaagata ttctgataat gaaagttgtt gctttccaag ctataaaact
      541 atcgctttga aatgcggatg tagtaaaagg caagccaaaa gcgtagttaa aattttagaa
      601 aataaagggt taattaaaaa agaaaataga ataaaaagta actcaaatga aaaagaaagc
      661 aacatatatt ttgttttaac agctaaatta ggtggtgaat atgatgcaca acaggtagtg
      721 aatatgatgc acaaccctag tgcacctcat gcacaacagg tagtgcatga ggtgcacagt
      781 aaaaagactt ataataaaaa gacttatata aaaagtaata ccactacaca aaaagaatct
      841 aaaactgatt atttagacct atcattcttg gacttagata tagaaaaagt aaaactaact
      901 aaagatgaat atgacaaact tataagcaag tttgggaaga aatacataca cgataaaatt
      961 gttagtttag aaaactatat cataaatggt aaagggagta ggtataagag ccactatagg
     1021 gctttgctga cttgggggaa tggagatgct agtaaaggta tagtaccaga agttacaaag
     1081 actaagaatc ctcttagtgg atttaaagaa ctttaaaaac taaatagtaa ggaggtagat
     1141 aggatgaatg aaaaagaaga gactgtttat ttgaatagtt ctaatgtgaa gttattctta
     1201 ggcgttaggg atattaagga atttgaggaa ttgatagaaa atgtcaatga gcgaatttgt
     1261 caactccaag aatcgattaa taaacttgcc caatttaata ttgagttcga tattaagtca
     1321 gatatttagt taagtataaa ttaggaggtt gcctatgaaa gatgaaaagt tagaacttac
     1381 tgaaaaagat ttacactgta ttgcaagaca tttacaaaat gaagttatgg agatggtgtt
     1441 tagagggaat atagaagccc ctacatcatg cgaagtttgc gattacttgc aagaatgcaa
     1501 ggactatttc acccatatag acacttttat aaaattaagt gaaatgacag gtgtagatat
     1561 ttttactaaa taaatttagt aaattaaaat tttagtattg taattgacgt gcttttttaa
     1621 aaagaggaca gttgctatta gcagtcatta tttgaatttt gcaaatgtaa tttttattac
     1681 attcgcattc taaaagtttt gatccagtga atccaggaaa agttttatat tcaccagaaa
     1741 attcaacttt atcgtcaata agagagcatt tttctcttaa ggttttaatt ttgcacatat
     1801 tcatcaccac cctataatta atttgaacat actgtcaata tgctccgatt aattataaca
     1861 tgcaaattat catgatgtag aattaaagca taggtcaatt aaatttagtt ataaaatcaa
     1921 tgaaacagat aaataattaa atgttaaata aattaggggg ttgttcatga aagaggaaaa
     1981 gttagaactt actgaaaaag aattgcattg tattgcaaga catttgcaaa atgaagttat
     2041 ggagatggtg tttagaggga atatagaagc ccccacatca tgtgaagttt gcgattacct
     2101 gcaagaatgt gagggagatt ttacctgtat gtatcattct tttactaaat taagcaaaat
     2161 aacaggtgta aaagtgactg catttaagta ttgttttcca gatgaaaatc ccaaagctac
     2221 tatctaagaa gtttttttag aataggacat tcttcattag aagaagagtc gcaaatatga
     2281 cttccatcac atctagttga cattaactta gatccaaaag tacctggagg agctgaatat
     2341 tcgcaagtaa gagaaatggt ttcgtttatt acaggacatt ctttgcttat aactcgatat
     2401 tggctcatac tctcaccacc tttctaatta atttaaacat attggaagta tgtttaaatt
     2461 aattatagca tgtaaattat catggtgtag aatagtttca aatcaaaaga agcaactaaa
     2521 tagttaaaga caggaggtat gctaagtgga aagaaaaaaa catgaattta cagatcaaga
     2581 tatacaaaga cttattgaag gattaagaac tgatccaaaa ataagagaaa tgatatttaa
     2641 aagtaaaggt tgtgaaacta gaacaagtac aagaagtaaa tgcgaacaat ttgttgaaga
     2701 aaaacttgct tatagaagtt atggatttga aactccaaaa tactatgcaa aacttattgc
     2761 cgacataatt gatattttag tttacaaaaa tgttccaaga gatcgtattt gtggggtatt
     2821 gtatgacgtc gaaagggtga ttcctttagt tgtgacattt taataataag ctgggggtga
     2881 ttaaatgaat tactctttag aagcagagca aaacattcta ggaagttttc ttataaatga
     2941 ctctataggt tacaaaataa gggaattaaa agaaaatgat ttctactttg agtcacataa
     3001 aattatttta tcttgcatga agaaaataat tgactcaaat aaaccactag atcttttgct
     3061 tttaaaaaat gaattagaaa aaatagatag attagtagat attggaggcg ttagttacat
     3121 taccagttta acaactatag ttataactac ttcaaacata gatcactata taaaaatcat
     3181 aaaagagaag ttacttaaaa gacaaatttc agagttagca aatgaattaa ttaataattc
     3241 aaattcagat acaagtatag atgaattgat tatagatata aacgatttaa aagcacttgt
     3301 gacgacgagc agtagtgtaa atgataatta cattgacgca tcaaaaataa aacgtgaaaa
     3361 aggcgtacac aagtccatag acacgggttt taataaacta aacaatatgc tagatggatt
     3421 tagatatgga actcttacaa ttttaactgg taaacctgca tctggtaaat caactatagt
     3481 taaccagttt atagcacaag caattacgaa tggagagaaa gcatttttgt attctggaga
     3541 attaccctct ttcatgtcta tggattggtt tagaaaaact gtagctaatg attatcacat
     3601 aaaagaatat aaaagtgtct atggaggaac atacacagat attccagatt acgccgttga
     3661 gcttatatca gactggatag aagataaatt ttttctctat gatgaagatg caataagtga
     3721 tgaagtaaat ctactaaata ccatagagca tttatattta aaaaaagggg ttaggttctt
     3781 tgttcttgac aatctaatga caataaaaac tggcaataag gcagataaat atgagagaca
     3841 agagcagata gtaagcaact taaaaaatct cgctaaaaaa tataacttag taatagtttt
     3901 agttgcacat cctcgtaaaa atatgggtga catgaagcca actatgtatg atgtgtctgg
     3961 agcaagtgag attgttaatt atgcagatta tattttatca acttacagag ttgttgatga
     4021 agaagaagag acagacgaca cttatttatt aattttaaaa aatagaataa caggaaaaca
     4081 gaatataagt tttaaaatga atttttcaga aagaagaaaa agactttaca cgactagtga
     4141 ggagttaaac agagattaca aatatgatgt aaataaacaa tatgttcaag tagaaattcc
     4201 agaagatgta ttttaaaaaa agtataaaaa ataggaaatt ttattaagta atgattttat
     4261 aagaaaaacc aatattgaat aatttaaaaa ttcaatattg aaaaaaatca tttttcaata
     4321 ttgaaaaacg gattttgaaa aagtattgaa tttcaatact aaaaaaagtt tgaaaatata
     4381 gcatttacaa ggtttatata aattaatttc ctgacaatgt acacacaggt ttagatatgc
     4441 tagcaaaaaa acttaaaagt agtgtaagga taaaaaggct atataaattt ataaaaagat
     4501 ttaaaagtgt tattaaatta gagctaaagt ttattaatta atttcctcgt agaaaaatat
     4561 tattttaaat aaataaagga gtgattttac atgatagcat tagaaaaatt tgcaggtgga
     4621 gttctaaaag aaaagtttaa tacagagtta caaaaagttt tagataacat tgcagatcca
     4681 aatacagatt ttaaaaaaac aagaaaaatt agtttagaga tagtgtttaa agcaaatgaa
     4741 gatagagatc ttgctgaagt agatataaaa tccaaagcaa ctatagtaga agcaaaggct
     4801 acaacaacta aagttctgat aggaaaagat ttagagacag gaagagttga agcgtctgaa
     4861 tttaaaaatc aagtagcagg gcagttggtc atggatattc cagatagtga agaaactaat
     4921 gaaacagaag aaaatagtgc tggtgtaatt gatttcagaa atgcaagttc aaagtagttt
     4981 aagaaagtaa gtaaaatata aatttaaata aaaaaggaga ataaaaaaaa tgataagaaa
     5041 tgcaatggaa tatttagtta agttaggaaa aagagaaaca gtatgtgtag atgggtttaa
     5101 gtacacaaca gatgcattag aaataataaa agaaccaaaa gctaatgaat tagaaataac
     5161 tacattgagt ggtttagtag attatataaa aagtggcata gatcataagg cagatgagtt
     5221 gttattaata caagttctaa ctccaagaaa agtgctttta aaatctcctt tgagaaaaaa
     5281 tagagataga gagacatata ttgaatgtgt tgcactacta ccagagaaaa gatttgacag
     5341 acctttagac ttagatagtt ttaacataat gctacaatca gcatttataa agaataatga
     5401 tagagataca cttttaaaaa ttgcaggaaa tatccaagaa tctacagtaa aaaatgtggg
     5461 agatgatgga gtttctcagt ctgtaacaat aaaaacaggc gtagctagtg tatcagaagc
     5521 aatagtccca aacagagtaa agttaattcc ttatcgcact ttccaagaga tagagcaacc
     5581 agaaagtgag tttattttca gaatgagttc tggaccagca gcaggattat atgaagcaga
     5641 tggtggagca tggaaaaatc aagcaatgtt aaacattaaa gcgtacttgc aagaagaatt
     5701 aaaagatttt aaaaatgtaa atataattgc ttaattgaaa atctaagagg gcttacaata
     5761 agtcctctta gtaaaaaagg aggggattag atgaaaatga tttctcatat gaacatttta
     5821 gaaagagcag aatttacaga taaaatagct aatgcaatca tgtctgcaaa taaagaaatt
     5881 gaaagaggct gtgcctttaa tgaagcagtt gagattgtaa aacaaatgga atatagagaa
     5941 aataaataca atgaaggaga tattgcaata tgaataatgt agttttagtt ggaagattag
     6001 caagagatcc agaattaaga tatataccag agtatggtac tccagttgct acttttgcat
     6061 tggcagttga taggggttat acaaaaaagg atggaactag agaagtcgat tttataccaa
     6121 ttgaagttat gggaggatcc gcagaatttt gtgctaatta tctcgcaaaa ggaagaatgg
     6181 tttcaattca aggccaaata aggatagaaa aatatgaaaa agatggagag aaaaaaactt
     6241 tcacaaaagt aagaacaaaa attgttaatg cgcttgatcg caagccaaag gatgaagaaa
     6301 aagaaattag ttttcaagtt ttagatgatg aagatattcc attttaaatt ggatggtgat
     6361 tatgtgagaa gtttagagga gagaaataaa ttagttgaag aaaatataaa ttttgtatgg
     6421 tttattataa gaagatattt caaagtattt atttcaaaat atccatatct tcaagatgat
     6481 acattttcag aaggatgtat tggattatgt aaagcagcag attattttga tagtaaaaaa
     6541 ggtattgaat ttaccacttt tgcagctgtg tgtataaaag gtcaaatatt taaatttata
     6601 agtaggtatg ttaaaaaaca ttacagagca gatatagata gttttgaaaa atgcatctat
     6661 aaaaatactc atggtgaaga aataagaata gaagatagat tggctggtaa tgaaaatgta
     6721 gatatagaaa atgttcatgt tattagatct tgtataaaaa gaagtaaaat agaagatata
     6781 cagaaaataa catacttaag ggaaaaagga tacactcaaa aagaaatagg caaaatactt
     6841 aggactaacc aaatgacaat atgcaggaag cttggaaaat taaaatctga aattaatata
     6901 ctaggaaaat aatataataa tttaagttca ttggaggcaa aatgaagact aaaataattg
     6961 gtggtaggga aaatcagtgc ccactttgta atggtagtgt atttaagatt gaaacatcaa
     7021 ttggaatagt atggcaatgt aaggattgtg gatgtatgta tcaagatatg aattgtaaag
     7081 aaaacaaaag gaggcattaa gtatggcttg tgttataaag tgtgattttt gtggtgaaac
     7141 aatagcagaa attctaaaca taaaggtatt cagaattgat gatgaaggta atataatgag
     7201 tatcggaaaa gatgtatgtg acaaatgtta tgacagatta tttagaagta acttgaataa
     7261 gaagatgact aattatgaaa agataaaaaa tatgagcaaa atagaaatgg ctgaatttct
     7321 tgcaaatggc ggaagtggat gtactagttg tgcttatgat tttcaagaat gcttaggcgg
     7381 atgtttagaa ggtagaaaaa aatggcttga aagtgaggcg atgaaataat gaaaaagacg
     7441 ttaataatcg taggagtagt tatttcaata ttaattatgt ttgcttgctt gttcttagta
     7501 actaaaaata aagcaattga tttagaagag caggttcaat cagcaactgc atctgtagag
     7561 gtgcaagaaa aaaggagaat tgatttaatt aagaacatag tagactgtgt aaaagactat
     7621 gctaaatatg aaaatagaac attaaaagaa gtaactgaca tgagagtaag tgcaaatgat
     7681 gaatctatca aggaagcaaa attaaatata caagcagtag cagagaagta cccagattta
     7741 aaagctaatg aaaattataa gcaactaatg agtgagttga gtataacaga aaatatgatt
     7801 gcacaacata gagaaaatta taatatgcaa gttcgcaatt acaataaata tataagaaaa
     7861 tttccagcaa atttaattct aaatagtatg ggatatgaaa aaatagaatc taaatatact
     7921 gaatataaag caaaagagga tgcacctcaa aatttatttg attaagaggt gcacatatga
     7981 aatttaaaac ttttgaaata actaagagag aatttttagt gagcataata attgcttgca
     8041 tgatgattgt gcttggattt ttcattgatg ataaagttca atcaaaattt ttagaagata
     8101 atgaaagata ttacaaagca actaaaataa ataatgacaa agatctcttt gattatgcta
     8161 tggaaacaag tgcgggagat tctttgataa gtggaagatt taaaagtgta gattctgtat
     8221 ctatagaaga attaaaaggg gactattggt ttatagaaag aacggaagaa aaatacacta
     8281 tgcatactag agtggttacc actaaagaca gtaaaggcca ttcacataaa tatactgaaa
     8341 catattggac ttgggattac catgattcaa tagagaaaaa atgtaataaa tttaatttca
     8401 tgggtaaaga gtttgatgcg aaagtaatta ctaatttatc taaagagaga ttggcattaa
     8461 agaaagatat atttaaaaat aataatctta aaataaaagg taattatgct tacattaata
     8521 ataagagaag gtactattat gaagttatac cgaaagaatt taatggtact atgtatgcta
     8581 atttaaaaaa taatactata gcgaatccag ctgataaaaa cataaaagtt tttaagggtg
     8641 agtctataca gaatgtttta gaaaataata attttaatac agggtttttt aaagtagtgt
     8701 tttggatcat ctggatatta ataactacaa tcattatata tgtattttat tatttaaaaa
     8761 atgattggct tgaagataaa taaagatgta agaggagaag atcaggtatg aaatgtaagg
     8821 tgtaccatgc tattaaaaat ataaaagatg caaatgagtt aattgataga tttgaaacgg
     8881 aaaatatatt ctttgaatta aatgacatag ataatgaaaa tgtcttaaat ggagaaatga
     8941 atttaactga tatagaagat attgcatcat tgcaatttat attagatgga gatttattta
     9001 tatttagtag ttgtgatgaa atgatattta tgaataaata cactcaaaaa gagttgggac
     9061 ttcaataaat gaatgagggg agatactaaa gatggattta aagttaatta gcagtatacc
     9121 taattggaag gtagaagatc agttggcaaa aattgatgaa gaggtaactg agtttaaaaa
     9181 tgctatagat acaggaataa ctaaagatat aattgcagaa ggattagatg tttgtcaaac
     9241 tattttaaca atgtttcaag ttttagaaat agaaaactat attactgaag gagtagagac
     9301 acataataag aaactaaatg gaagaggatg ggatctcaaa gaaataggag acgatatact
     9361 taatttaatt aaaaattcaa tgattaatga attgattaat atgaacaaga aattaaaatg
     9421 ccttgatgaa aaagaattta atgcaaaaat acaattttta gatgtagata aactatggaa
     9481 tttgtgcatg cctttagtta acttaagtac tataaaagga gttaatgaaa gcattgatga
     9541 aatgaaaatg ggagagtttc taaaaaatat taattaaagt aaggagataa tataatatgt
     9601 tgatagaagt aactgaaaat aatagagaat taaaagtagg agatattgtt gaaattaatg
     9661 gtggtaataa gttatatatg attctttctt taaaggaaac aataggtggg tatgttatta
     9721 taaatatgca gaatggatat ggttcatttg gtgcttataa aagtttaagt caactagaat
     9781 tagacttgaa ggtaagaggt tataaattat atagttctga tgattacaag ttgcaattag
     9841 tgcctagaga ataagatata tttcaaaaaa gaaaaaaagg agcattactt cacgctcctg
     9901 cttgtcaaaa ttctaaaacc gtcatcacaa catcattata acataattag gagtgtgagg
     9961 taatgcaatc tagtaaaaaa gacaatttgt ttagtagtgc agaaggtaag ttatatgatt
    10021 ataaaaaaat aaaagcggaa atagaaaaaa tagacattga tatgcagatg ataaaaaacg
    10081 actacagagg atgtagtgga gttgaaatta aagaaaagac aggcaagaca tatgatatta
    10141 aaagtgtaat tgaaatagag actgaagaaa aagaaaagaa aattgcctta aaagaaaaag
    10201 agaagagaca taaagagttg ataatagaaa aaatagataa tgcaatgaaa atattaagtg
    10261 aagaagaaaa aaagatagta cagtataaat acttttcaag tagcaggaca tcttgggaat
    10321 atgttggcag aatgattggt ttttcagcga gtaagtgtaa gcaaatgagg tttgatatta
    10381 tagataagat aaaaggattg ctataaaaat gtccgatttg tgacgatttt aaaccgattt
    10441 atgtctgttt tgtgtctaaa aaatgtccgg tttgtgacca atttatgtct gttttgtgtc
    10501 ttttaatcat gataagattg tattgtgaga aaaatatatt taataaaaat ggcttggaat
    10561 ttacgttcta agtctttttt attgtctaga gaggtgttga agattaaacg tgatataaca
    10621 gaagaaattt caagtgctgc atatgtacca gacaatctaa agtattacga caatgtaatg
    10681 aaagaaactc ctggtatgta tggaagagaa tgttctttag attttataaa gaaaaagcaa
    10741 gaaaaactct tgaaactaaa aaagaaggtt aagaaaaatt atgattctaa tattaataag
    10801 atagactcat atctaaaaat tttagagaaa agtataattg atgagtctga tcacgttgag
    10861 ttggttgtat ttaaattata tttgcaatta gaaaatgttg taaaagtaac tgaaactgta
    10921 aacgacttag gatttagaat aaagactaac acttatgcaa gagaaagaaa atatagtaca
    10981 aatgatataa cctctataat cacagatcct tttactgatg tagttgagga cttaaaaata
    11041 ttagttcaag agaggcaaag aaaaaattat tatggaaata aggagtgttt ttaatgaatg
    11101 gaagcacata gtggacaaaa ggttaatccc ataaaagata tagaggatgt atttagactt
    11161 ttaaattttc tagaagaatg gaatgaaaga aactatttat tagccctctt tggaatgtgt
    11221 actggactta gggtagggga tatattagca cttaaagttg cagatgtaac agacttaaaa
    11281 ctcgataaaa aaggaaataa aataagagta tctaaggatt ggattagagt tatcgaagaa
    11341 aaaagagact ataatagaga ggttttttta tctgatgtaa taaaaagtgc tatagaaaat
    11401 tatactcaag ataaaccagg agaagaattt ttatttaaaa gtggcaagag gaagaaattt
    11461 aatagaccta tccaaacaag acaagcagga agaataataa aaggagcagc aaaaaaggta
    11521 ggaataaaag aaaatgttgc aacacactct ttaagaaaaa catttgcaag gcatatttat
    11581 gatgaagaag agaataagac ttatgcatta gaacttataa ggaagattct aggacataag
    11641 acaatagaaa tgactagaag atatatagga atagataagg atgaagaagt aaaagcaata
    11701 actaaattta ctaataagtt aaagaaaaga aaaagaaatt aaaatatctt gtattttatc
    11761 taaaatttaa tatgttacgt ttttcaacaa caggacatta ttagaatgca aagagcctta
    11821 acaatagagt ttacaaggaa tagaatagat ttattaaaat gtctgaaaat atatattaaa
    11881 ggtcatttat tcaagggatt tatagggatg tagatattta aatgtttatt tagaagctga
    11941 gtaaagaggt gattaattgg atgtgaatga agttgaaata atcgatagta gctatgaaaa
    12001 aggtaaagag ttgacagaag atcagtgcaa tatggtaact ctattgatta caggtgctac
    12061 agtaacagaa actgccagaa ttattggagt aaatagaaaa acaatctata attggatgaa
    12121 taaagagcat gttaggaaag aaatggacag acggaaacag gagctaacaa accaaggaaa
    12181 tttaatgata ttaaaggatt tagatagtta tatcaacaat atcaaggaat tagctagtga
    12241 caagagcgat aagagggtta tgttggcagc caatcagtat cttataaatc gtatatatgg
    12301 aactcctaca agtacagtta tacaagcaga ggataatagt gttggcatgg gtatggatgc
    12361 aacagagata gaagcagcta tagctaagat aagaataaga acaagcaaga agtaattaga
    12421 tatgaaataa atcctgcaag aatatgtttt aattatgttt gaaatcaatt aaagcaaaac
    12481 atatattata tatatattta ctttaataat taaatcatat attgtattta actttagtta
    12541 tagctaattt aataaaattt agtgagactt aataattaaa gtttcataca gtgaagtgat
    12601 gtggattatt ataggatatg tgaacctatt ggaaatgcta ggttcttttt aactatgccg
    12661 aaaatacata tttaaggaat agttgaaaga taattaataa ttctaatcaa aaaaatattt
    12721 cttgcaagaa aataattcaa aatactttaa tatatttatt ttttttatta atagggggtg
    12781 tccttctatt ttgaaaaaat tattttttat ggaaaatgag ttctagaaat tttctagcaa
    12841 aatttaaaaa ccagagatgt atttcaaaga gggtgatttt attagagaaa aatttacatt
    12901 gtttatttta ttaattgcat tactcactga gcctaaaaaa atagaagaag taagaggaag
    12961 taaatacgag tggataatct agaaagaaat atacaattaa tatttagtta tctagcaaag
    13021 acttttataa gaaatggagc atctataaaa gatgctgaaa aagaagcaaa tgagattata
    13081 aaagccaatt caaacaatct ttggggagtt aatggtctcg cttatcaact tggaaaaata
    13141 aacctagaat ttttctgtat gtattttatg caagacacat atttaccaaa agaagataat
    13201 gctgctgctc ctatagctaa agttcatcat gagctatggg aagatataca agaatctata
    13261 attggagatg gttcacagca attaggaaga atttatccaa gagggacagg taaaagtgca
    13321 tttggagatt tagcgacaac tgtatggtcg cattgctata aacataagac atatactttg
    13381 atttgctctg acataggatc tacagcagag aaatttgtaa aagatataaa aaatgcatta
    13441 cttgaaaatg agtatattaa aaaagcattt ggtgtccttt taaatgataa tgatagaaaa
    13501 tacatttgta atagtactca actagagcta acaaataaaa cttttattga agctatatca
    13561 tcttcatcac ccatgagagg aagaaaatac aataataacc gtccagatct tatcatactt
    13621 gatgattatc agtcagaaga aaatgttaga acagaagatg ctagagagaa aaaatttaaa
    13681 agattttctg atgatgtaaa atatgctgct caaaaaccag tcataagaaa tggtaaaact
    13741 ataaaaagag gaacaacttt tatagcatta ggaaccttac aacacaaaga atgtttttat
    13801 agtagactaa aaaatttacc aacttggaag tttaaatgcg aaaaaggagt tttagttgat
    13861 aatgtagatg aattatttaa ttctggttta tggcttaagt tcaaagagat cttatttgat
    13921 tttaaaaata caaatcatct tgaagatgct aaagaatttt actgggaaaa tgaaaaagaa
    13981 atgaaattcc atatattatg gccaagtttt tgggattgtt tagatatggc tctaagttat
    14041 tatgaaaatc caacatcttt taagcaggaa gtgcaaaatg atgtcaattc tataggagaa
    14101 aaatggttta aaactgttag aacagaaact agagaagaaa ttgaatcgca tacttttaaa
    14161 aagacaatgc tgttatgtga tccagcatct gcgggtgggt cgaaacatga ctatagcgct
    14221 tttcttgttg gaagtgaatc agaaaatggt ttactatatg gaagattggc agaactagct
    14281 aaaataaatg ctagaactga ttttgataaa tatatagacc atatgattta tttattaaaa
    14341 gtgtattcag atataacaca tatttatata gaaaaaaata catttaatgg cgcagatgcc
    14401 aaccaattag agtttaaaat aaaaaatgat aatgttctta gttatagaga tatagaaatc
    14461 attaatgagc aacaaaagaa aaataaagat gataaaatct ctactttaat acctgtttta
    14521 agcaaaggcc aaatgatttt tgcagaagaa gataaggaat ttatacagca aatacttgat
    14581 tttacaggac aaaaatactc tctacatgat gatgctccgg atatatcatc ggaatttatt
    14641 aatcgaatta acaacataaa agtttctgag acagtaaata tctttgatag aaaattatta
    14701 ggtttttagt tgggaggtaa tttattggct aatagatcaa agtttgaaga agttcaaaaa
    14761 actttaacta aaaaaggaaa gaagttcaat gttggaatag gaaaagatga aaaaggatat
    14821 tttgcatata ctcatagagc aaggagtaaa agttatattt caaagcaaga tattccaata
    14881 aaagttttaa aatttataga gtctacagga taggatgtgt tgttaatgga attagattta
    14941 gacttaataa aagacatata tgacgattgg aactctaata aatccgaata tgatactatg
    15001 tataagtatt ataaaggtga aacggatgct attagcaact ataaaatggt taccaagaga
    15061 tctaataata agataaacac taatttctta aaaaagttta ttaatgaaga agtcgcttat
    15121 tctcttgcaa ataagattac ctatacaagt agatctggtg atgagaagat tataaatgat
    15181 ttagaatact atacttgcca ttggagtaaa aaacatgact cagatttatt gaggtatgca
    15241 ctattatttg ggctttgcta tgaactttac tatgtaaaag atgatgaaat acaagtaaag
    15301 ataatcaagc caacagatgg ctgtcattat gaaaatgaaa atggagaaat agtatgcttt
    15361 attagagagt ttaccaaagg tttcgaagat gacacgtatg tagatgtata tgacaaagaa
    15421 tatatatatc attttgattc aaatttcaaa gaagttgaaa gtcctacagt aaacaatata
    15481 tttgatggaa atgttccaat atcaatttgc aaaagaagtg aagaactagg aaaaaataca
    15541 atatttaatg atataaaagg gttacaagat gcttatgaaa ctaatttaag tgatataagt
    15601 aatgaaatta gtgattttag aaatgcttat ttaacctttt ctggatgtaa tattaaagaa
    15661 gatgatttgc caaggatgaa agaacttgga atacttcaag ttaatggaga tggaaaaata
    15721 gagtggctta ttaaagatat gaatgataca tttgttcaaa atacactatc ttcaataaaa
    15781 gaaaatatgt atgaaattac atctcatata aatcacaatg aaaaaatgca aagtaataca
    15841 tcaagtctag cagttatagc aagattaatc agtacagaat ggatttgtag ccaaaataat
    15901 gatagtattg cagatacttt atttaatagg tacaaattat tgtgcatttg gcttaataaa
    15961 aaatatggtt ttgactatga ttataaagat attaaggcta aatttacccc taagatacca
    16021 cgtgatgatt tagttgtagc aaatatatta agccaacttg gagataaatt atctactgaa
    16081 actggcttat ctcaactaag tttcattgat aatcctcatg ctgaaataga aaaagctaaa
    16141 aaagaacagg aaatagtttc agagggagag attttattag atgaatcaaa agattataac
    16201 taaattgact gaagatatat ataaatctgc tgaaaatagg acaaaaccat tttataagta
    16261 tcaaagagaa aatagggata aacttttatt agaaatagca aagatattgc taacacacga
    16321 tattattaat gaatacttaa atattaataa taaagataag aaaaatctaa ggtcaaaact
    16381 taataaaatt ataaatgagt attctaatag ttatgatgaa gaagtcaagg atatagaaac
    16441 tattttatct gattcatcta agaataaata ttcaaatttg ataattttat taggagtaat
    16501 actgaagata aaagatgtta gtaatataaa caataaagtt ataaataaga tagtgaataa
    16561 taaggttgat ggaaaacatt ggagcgatag actctggaag aataaaaaag gtatagaaga
    16621 gtccttaaaa aaagaaataa agagttttct aaatggaaaa acttctgtaa ataagataga
    16681 aaaagcaata agagataaat attcaacagg tgcaggacaa accagcagac taatagaaaa
    16741 tgaagttgcc agatgtcaaa gtgaagtaaa tgagtatttt ttcaaggttc aagggattat
    16801 aaaagtaatg tattgtgcag atttagattc tcgcacatgt agtgattgtt ctatgcataa
    16861 tggagtgata ttttatgtca atgaagatag gccctctttg ccaagacact gtcactgtag
    16921 atgtcaatat atacctgttt aacatgtatt aattaattta ataggtgttt ttattatgtc
    16981 taaaaaatat gaggtgaaat atgaaagttg aaatattagg aacagaatat ctagtgatag
    17041 agaaacttga aagcgaagat gtgctattaa aggaaagagc tggatattgt gatcactctg
    17101 ttaaggaaat tgttatagag aaaataaatt ctgaagaagg ttcattgaaa gatttaagtg
    17161 tatataaaaa tgaagttgct aggcatgaaa taattcatgc atttcttagt gaaagtggat
    17221 tgaagagttg tagttcttgg gctacaaacg aagaaatgat tgacttcttt gcaattcaat
    17281 ttcctaaaat agtaaaggtt atggataaaa tagaatgttt gaattaaatt ttcaaaaagg
    17341 agaactttat acatgttaaa aaaagaagtg ttggaattac taaaagaaat agaagatgat
    17401 gcagaaatag attcattgct gcaatcaaca gatttattca aatctgcatc tgataaaaag
    17461 cttacaatag aagagtttag agaattaatt aataatgatg caaactttaa agctattata
    17521 gataatgaaa aaaataaata tcacagcgaa gcacttgaaa actttaagaa aaaagatatg
    17581 cagacactta taagtgctga agttctaaaa agaactggag caaatgaaac agaagagcaa
    17641 aaagcaataa gggaattaag agaaagccta gacaaactaa aaaaagaaaa gcagtacgca
    17701 gaaaaagttt ctaaatataa agatatttta gttgaaaaga aaatacctac aaacttaatt
    17761 gaatatctat tatctgatga tgatgataaa acaaatgcaa atatagagat atttgaaaat
    17821 tcaatgaagc aatatgtgca gtcaaaggta gatgaaagaa ttaaagatgg ttcatacgtt
    17881 ccacctggca aagatagtgc aggttcgcta tcagaaatta gaaaacaaat aaagcaaggg
    17941 ctaaatagtc tttagtataa aaaaagaaag gatgatttta taaatggcta atgtattaca
    18001 atatgtaaca ctttttcaac aagaactaga tatgcaggta acagttggct caactactgg
    18061 atggatggag aaaaatgctg gtcaagttat atataatgga ggaagagagg taaaagtacc
    18121 aaaagtaaca atggatggct taggtgatta tgacagatct aaaggatata cgcaaggcgc
    18181 agttacactt gagtacgaaa caatgaaaat ggagatggat agaggtagga cttttatgtt
    18241 agattcaatg gatgtaaatg aaagtaactt cgttataaat gcatcttctg taatgggatt
    18301 atttcaaaag caacatgtta taccagaaat agatgcatat agatactcaa agatattttc
    18361 tgaaacagct aaagtagatg agcgtgtaaa atattcatac gaagctacag taactacaat
    18421 attaagtgaa ttgaaaaatc aacttgcatc aatacaagat ttatctggag atatacctct
    18481 tattattagt atgtctagca taattgcaag catattagaa caatcatctc aacttgatga
    18541 tattgaattt ggcgatggag atataaaaag aattgtaaag tctatagaca atgtacctat
    18601 aataaaagtt ccatcaaaaa gaatgaaaac agaatacgta tttagagatg gtaaaacatc
    18661 tggacaaaca gagggtgggt ttactgaagg agcaaaggct ttagatataa actggttaat
    18721 aactccagca tctgctccaa ttgcagtatc taagactgat aagccaagaa tatttactcc
    18781 agatcaaaac caaacagcag atgcttatca aatagactat agaaaatatc acgatttgtg
    18841 gatattggat gaatatttaa aactttgtag agcatctgtg agacaagcta aagctgcaca
    18901 agaataagga gttgttttaa atgtttgcat tgataaaaaa taacataatt aaagttgttg
    18961 aagatgaaaa ttcgagagat aaattgattt caaaaggatt taaaatatat tcagaagaag
    19021 tgactaaaga gaatattgag aaaaaagaag aaaaagttaa atcaacctct aatgatttat
    19081 ttaatctatc taaaccagag cttatggaaa ttgctaaagg caagaatata aagggatatt
    19141 caactaaaac taaagaggaa ttaattcaac tactagatga gtacaatgct tgataatgtt
    19201 aaattagttt taggaataga aaatgataga tatgacaaat taataatcct ttatataaat
    19261 aaaataagtg agatggtttt agaatattgt tctaggcagg aactaagtac agcactagaa
    19321 gggtttgtag aagaaaaagt aatatcaata ttatcttcaa gatttgaaga acttaagaaa
    19381 aatagtggac ttgataatct agaaaaaagc ttaaaagcta taactagagg tgacactaag
    19441 atagaatata atgctttaac attacaaact gctgaaaata gcatcagttc atcatctttt
    19501 ttaactgata ctgataaaaa atttctaagc ccattttgta ttgttagtat gtactaaggg
    19561 gtgattaaat gactgaagca gatatattag aaatgacata ccttgataaa atgactataa
    19621 ttagaaaatt aaaaggctgg aatgaagaaa catgttctaa tgattttagt aatgttgtta
    19681 tagctgaaaa tatcccatgt gcaatttcta gaaaagaaga agccattgta aatggtgata
    19741 ttggatctat agttgtaaat agaaagttat tttgtagacc agaagtagat ataaaaatag
    19801 gtgatactat aaatacaacc tataatcatg gagaagaagc tgtatttaag gtttctaagg
    19861 caaatatata tccaagccat atggaaatac ctattacaga attggagagg atataatggg
    19921 aatagataat ttaggagtct ttgaagcatt agccttagat ttaggtctaa atgcatcaaa
    19981 ctttaataga aaaatcaaga agtttacaaa agaaactggg caagaactag cagaaaatat
    20041 aagagatgaa actcctgtaa aaactgaaaa gttaaaaaag ggatgggaag aaaagcaacc
    20101 agaatatgat acttatattg ttaaaaatga agttgattat gctactttag taaataatgg
    20161 ccattcaact aaaaatggtg gcattgtccc tggttctttt atggttgaaa agggaattga
    20221 aagaacaaaa gaaaatatga caagtgcaaa agcaaattta tttgattggt aggtgattag
    20281 ttgcttacaa acagagacat tataatagca gttaataaaa gaatcgaaaa tgagtttaga
    20341 tatatagata tttttattaa tgaggataat atacaaggct ttgaggaggc ttgttttttt
    20401 gtgcaacttt tacctgtttc taataatatt gcaaataaag ttttagatac taaaaagata
    20461 ttcatagata ttgaatatta ccaaaaggct agaaaaaata aattaaatct atacgacata
    20521 caaagtagat tagaaaaaat atttaacaga aacattagag ttaaagatag aagtttaatc
    20581 attgagaata tatctccatc aatcgcaaaa gatcctgtag gatataaact aacttttcta
    20641 atgactgtat cttattttga agaagtctat tttgaaaatg aaacatttga aattatggaa
    20701 agtgtagatt tagagatagg aagtgattaa atgggtttac cagaaattaa tatttctttt
    20761 atccaagcag gaaaaacttt tgttaaacgc tctggtggta ttgtagcgct tattttaaaa
    20821 gatacaaaaa atatagggtt agttgagata aaagaaatag aggatattcc agaaaattat
    20881 tctgctgcta atttagatta tataaaaatg gctatgaagg gaaatgtata tcctccaaat
    20941 aaagtattag tttatacatt agatacagat gaatcaatag atgatgcact agattttctt
    21001 gaaacttgtg agtttaatta tctttgtatg ccagatgaga cagaacaaga tttgcctaaa
    21061 atcaaaacgt ggattaagaa aatgagagaa gataataaaa taaaggttaa agctattact
    21121 gcaagtaatt cagcggatta tgaaggtata ataaacttta cagcaactga tattgaagtt
    21181 gaaggtaaaa aatacacttc aaatgaattt ctgccaagaa tagcaggttt tattgcagga
    21241 actccatcaa ctcaatctgt aacatatgca gaaataccag aagttactaa tatacctaag
    21301 ctaacaagag ctgaagcaaa tacaaggata aataatggag agctaatttt aataaaagaa
    21361 agtggagcta tagtaatcgc tagaggagtt acatccttta caacaattac tgattcaaaa
    21421 ggtgatatat tcaaaaagat taagttggtt gatacactag atcaaattca taacgatatt
    21481 aaaaaaataa ttgtaaaaaa ttatatagga aaaacccaaa acacttatga taataagtgt
    21541 ttacttattg ttgcaataca attatatctt caagaattag aaaaagatgg tttaattgat
    21601 gaaggttcaa ctgtagaaat taatttagat gctcaaagag catggctaaa aaaacaaaat
    21661 atagatatat ctaatatgga agaacaacag ataaaagaat acaatactga cactcttgta
    21721 tttttaaaag caagaattaa attaatagat gcaatggaag atgtatattt agacatctct
    21781 atgtagtttg gaggttaaaa tttgaaagct aaaatagatg caaaaagaat aatatctggt
    21841 acttttggag agtgttggct agataataag caacttacat caacaaaagg gatggaggcc
    21901 aaggttgata tagataaaga agaaataaaa ttatgtggtc aaataatgac cacatataaa
    21961 atgattggtg caactggaaa aggatctata actctttata agataagtac cttatttggc
    22021 gagtatgtcg aaaaaatgtt aaatgaaggt aaagaattta aagtaactat aataagtaaa
    22081 ttgcaagacc cagattcata tggttctgag agaattgcac tttatggatg tatgtttgac
    22141 gacttaactt tggctgattg ggaagctgcg aaagttggag aaatagaagt tccattcaca
    22201 tttgaaggct ttgaatactt agataaaata aaagaaaaat aggaggattt tattatatga
    22261 acacagtaga aaaattatta aatatggatg caggtaaatt aaaaatgcca tctagcgcat
    22321 ttgaattata ttgtaaaaaa ctagatgata ctttagaaat agagtgcaag gctattgatc
    22381 cagaacgttt tgacgaaata agaatgaact ctatggacgt taatagtgga gatgtagaaa
    22441 acattaacat atatgaactt aaagtaaata caatattaga atcatgttct atatttagca
    22501 atatggaatt agttaaaaaa tttagtgcaa ttacacctaa agaattaatt aaaaagctat
    22561 tacttagtgg tgaaatagat aaattatacg aggaggttaa taaagtaaat ggaatagatg
    22621 cagagggaga taaaaaaaga caaaaagaaa gagctaaaga tataaaaaac taattaaaac
    22681 ggatggagaa gttaacttga tgtacttatt atttaagtat aaaggtataa tgccatccgt
    22741 ttcttatcat atactaagag gcgaaaaaga aattgttagt gtttttatgg atcaagaatt
    22801 agatgaaaga aaagaagaaa taaaatctgg tgttgggagg ttgttttagt atatgagttc
    22861 taatacaaag gtcctacaag caataataaa aatgagagat gaagcaagtt caacattaaa
    22921 aagagtagca gcaaatacta gagctctaca tgattcatct tcccaagcat caagagcatt
    22981 aaataatgtt caaagtaatt taaaaagtgt tggaactagt gctttaaaag caggaggtct
    23041 tatcactgct ggagttggtg gtgcatttgc agctgcaact aaagttggaa cagaatttga
    23101 aagcgctatg tcgcaagtta aggctacatt tggaaattcc ttaaaaccag gagattttga
    23161 agctctgtct aaggctgcta gagatgctgg aaaagctaca actaaaactg ctgctgattc
    23221 tgctaatgca cttcagtata tggggcttgc tggatgggat gttcaaaagt caatgcatgc
    23281 cttaatgcca gtgcttaatt tatcagaagc agcctctatg gatcttggtc tagcatcaga
    23341 tttagtaact gatagcatga gttccatgtg cttagaagtt gataaggtca gtaaaagtgg
    23401 aaaagtacta gctggtgcag gtttaagtga gtatttagat aaagttgcaa atgcatctac
    23461 taaatcaaat acaagtatac aacagttgat ggaagcgttt aatgtcgcag gtggtacatt
    23521 tagcaattta aaagttccac tttcagaagc aactgctatc atgggaatac ttgcaaatag
    23581 aggagttaag ggtacagatg cagggcatgc tttaaactct atcatggtaa atcttacaac
    23641 aggtgcaggt caagcaggta aggcaatgaa aaagttaggt gtaagtgcat ttgataatca
    23701 aggtaaattt aaaggtatgt caaatgtgct taaagaggtt gcaaataaaa ctaaaggtat
    23761 gactgatgaa cagaaaaatt attacttatc tgctattggt ggaaagactc aattaaaaac
    23821 gttacaaaag ttacttgatg gtgtgggaaa tgaatatgat agcttaaaga aaaaaataga
    23881 taattcaaaa ggtgctttaa aaacaatgtc tgatgtaatg aaggataata ctaagggtgg
    23941 acttgcaacc ttaaaatcag cccttgaaga aactggaatt actatttact atcaactaaa
    24001 gccagcaata gcatctgtag ttgataattt aactaaaatg actgtttggt ttaataatct
    24061 aagtgaacca ataaaaagag tcataactaa tgtaatgctt tcagttggag catttggagt
    24121 acttttatta actatagggg ctgtgtcttt aattgtttca aaagctatag gtatatttgg
    24181 cttttttgtt ccaataatat taagagcaaa agttgctttc agagcttttg taattggtat
    24241 gaaaccatta agtgcagtat ttttagctgt aatgggtcct gtgggatgga ctataacagc
    24301 tgtaattgca ttatcagtag catttaccat tgcttatgct aaatctgaag tatttagaaa
    24361 taaggttaat tcattagtaa aatcatttac agaatttgca aagcctatag taaatttttg
    24421 tatgcctgca cttaaacagc taggagagaa atttaaagag ttgttatcag catttggtcc
    24481 actatttgca tctattttaa acctaggaag tgtaataatg aaggtactag gacctgtttt
    24541 aatcggcgta ataggttttc atatagcaaa attagttttg attttatcta ctctggcaac
    24601 tgcttttaaa gctgtagttg atactataac taatgtatta aatggtctag catctatttt
    24661 aaatggagta tttgatttaa ttataggtat tgtcacaggc gatagtaaaa agatgatgga
    24721 tggagttaaa caaatatttg atggtggaac taaaattata aaatcaattt ggaaaggttt
    24781 catagatttt gtaactgctc ctatacaagc tatagctgat atagctgaca gcttatttaa
    24841 agataaagct aaatggctta cagataagtg gagagattta agaacattct tagaaaatcc
    24901 aattgaagca actgccaaaa tatctactaa aacaaatgta aatatcccag gaagtaaagc
    24961 agcagatgca gctggtaaat ctacaccaaa tacaggtgga ttaaaacaaa cagtagcaaa
    25021 tctaaaaggt tcatttagtg attttaaaac ttttattaag cctatgacag actctataag
    25081 tcaaggagtt gaatctggaa aaactaaatt tacagaactg caaaataaaa tattaaatga
    25141 tgttaaacct gcactaaagg tcctagagga ttctggagta aattttaagg ataagtttaa
    25201 ggagtcaata acttcaataa aagataaatt taatgaatta aagaacatat tagctggaaa
    25261 tattaaaaca aatatagata atttagtaaa agcatttgaa cctttaaaac ctcatttaga
    25321 taatttgaaa gattgctttg ataaggttaa aaaagcgatt actgattttt ttactccagc
    25381 caaacaggtt ggagatacag taaacaaagt aagccaacca atggatagtg ttaaaaagtc
    25441 tactgatgga attaaacttt ctttccaaag tttaactcaa gcacttgctc ctgttaaagc
    25501 ttggtttcaa ggatttatgc ctttcctatc acaaatagga caacaacttt taccatctat
    25561 aggaattgca atagctggtg ttgttggcgg aataatttta gcatttacat gggttttaaa
    25621 tacattgacc gcaataataa caacagtatc tggaatcata aatggaatta tgtcttttat
    25681 atctggaatt attaatgtta ttatggggct tataacagga aatgctgatc aaactatgga
    25741 tggacttaaa caaatatttg atagtggagt aggtgtaata aaatcaattt ggaatggcct
    25801 tgttgatcta gttagtggcg tactagaacc tgtaatagat cttgctgatt ctcttttcaa
    25861 agataaagtt aaaggggtaa aagaagcttg gaataaactt aaagatttct ttaaacatcc
    25921 aattaaggga acaatagaaa tatttaaaaa aggtcctagt ttacaaattg gaaaaggtgc
    25981 agatgctgct ggcaaaagtc caagaaaagc atttggtata aatagagttc ctagaaatga
    26041 ttatttaata agagcacacg aaggcgagaa acttttaacc aaacaagaag ctaatcaata
    26101 caaaaggaat agttcaaatg gaagtttatt tcttgataaa ttggctgata caataattgt
    26161 aagagaagag gctgacatag ataaaattgt aagaaaaata aacaaaaaaa tatctcttgc
    26221 aaaaattgga ggtgttttgt aaattatcgt gctataattg tagtatataa tttacagggg
    26281 gttatgttat gaataaaaaa atactagtat taacaatatc aattatacta tctttaagtt
    26341 tagttggatg cagcacaaat aattcaaaaa atagagatag taaatctaag caaaacacag
    26401 aggaagttaa aaatcataaa ataggcgaag ttgttgattt agatggagca aaagtatctg
    26461 ttaaaaatat taggaaatta gaaacaaata gtaatgatta tgctgttgaa attgaaataa
    26521 ctaatacagg tgataatgat ttacaaagtg attatctaga aaactatata ttaaaatcaa
    26581 gtgacaatac tactggagaa gcaataaata accaagaatt aaatggagag ttaatgtttg
    26641 atacaatacg cccaggagat actttgaaag gtgaaatagc attcgaactt acaaaagatg
    26701 caacaccaaa aacacttgaa attgcttatg aaaatgtagg caaatataca gtatttgatt
    26761 tatagtaatt atcttaaaag acgcttattt acttaagtgt cttttatttt aggaggagtt
    26821 taatggaaat atggttaaga caagcaaata atacatttag gtttcctgtt attccaccct
    26881 cttttgagat aaatggaagt gcgacaataa atacatctaa tattttaagt gttggagata
    26941 tcgcagtatt tggaggatta gggcttaaaa ctatagaact atcctctttt ttccctaatc
    27001 aagaatatag tttctgtaat tataatggat ttccaaaacc atatgattgt gtaaatttaa
    27061 tagaaagttg gatgaaagaa ggatatatat taagatttat cattacggaa actaatataa
    27121 actttgaatg tataattgca gattttaatt atagagaaca agattattca agagatattt
    27181 attttacctt aagtctaaaa gaatacagaa ggatacaaat atccaaagta aacattaata
    27241 atgatgaaaa attatcttct gaaaagaatg ttcctctaac aaaaggattt gatactaagc
    27301 aaaaaacgca taaggttgtt gaaggagata ctctttttaa gatagctaaa aaatattata
    27361 gcaatggaga tttatgggag aagatttata aagcaaatga agatgagatt aaagatccat
    27421 ctgtaataaa aaatggttgg gtattaataa ttccttaaaa atggtggtga tttataatta
    27481 ataaaatcaa aatacaagtt catataaaaa acggaaatat atataatgta actgatttag
    27541 tggataaatg cacttggtct ggtgattata gatcaccatc aagaactttg gaattttcta
    27601 taattcaatc tgcaagtgat attaatttta ggcagataga tattccagta gcaagtacag
    27661 tttgtttcta tgtagatgat aaagaacttt ttagaggaat gataatagat agatctaaag
    27721 actctagcaa taataatata gattttacag ctaaagatat gggtttttta cttttacaaa
    27781 gcgaagtgtc atacaatttt aaagataaat tagtcgaaga tattgcaaaa caggtttttg
    27841 tagataataa attcccacta ggtaatttac ctaaaactaa cgttaaatat acaaaaatgt
    27901 ttattggtgt aactggctac gatactataa tgagcgttta tacagaagca agcaagacaa
    27961 ctaaaaaaaa gtatatgata gagtctaatc ttgataaatt taatgttatt gagaagggtg
    28021 tagttacttt aaatataaca tttgaagaag gctttaatct tatcaataca aatttttctg
    28081 aaagcattga gaatgtaaaa aataaagtac tagttgttga tcagtatggt aataaaatta
    28141 gtgagaaaat aaatgactct atatttaaag acgttggggt aattatgcaa aaggtaatac
    28201 aacaacaaga aaacagtact atagacatag atggagagtt taaaggaatc gaaaaaactt
    28261 gctctttaaa aggatatggt gatataactt gtgtaactgg tagaggtgta aaagtaaaag
    28321 actcatatac aaaattaata ggtctttttt atatagatac agataagcat acatggcaaa
    28381 atggagatta tcaaattgaa ctagagctta attttgagaa tataatggat gaaaaatctg
    28441 ctggccaaga tgagcaaaaa gaagatggtt cagagtatac tggaggaaca gaataccctg
    28501 ctgaatttac ggcttattgt cctagaaaag aagagggtgg aaatacagat tgtagaggta
    28561 aaaaactaga tccttctaaa aagacatgtg ccgctcctat ggttggagca tatgaaaaat
    28621 cttactatac aaaagatttc ttaaacaaac atcctcttat gagatatgga gatgaaatac
    28681 aattagttac aggtgtttca agccgagatg gaacgtataa agtaaatgat aatgggcctg
    28741 caataattat agaaaaggat ggaacatatc atatagatat tttatttgga aatgttgaag
    28801 aagctaataa ctttggaaga agaaaaggaa agattataat tggtggttat tctggaaaca
    28861 tgacagaaaa agcaaaaaca gtaatttcag aagcaagaaa acatcttgga aagccataca
    28921 aatggggtgg gaatggacca agtagctttg attgttctgg actaatggta tattgcttta
    28981 agaaggttaa tgttaattta ccaagaacct caagtcagca atctaaggta ggtaagaaag
    29041 tagagaaaaa taatttacag gcaggagatt tggtattttt ccataatcca gtcagccatg
    29101 ttggactata tgtaggtaat ggagaatttt tacatgcgcc acaaacagga gatgtagtta
    29161 aaataagtaa attaagtaat agaaaagatt ttaatacagc tagaagagtt ttgtaaaaga
    29221 ggtgatatcg tggcagatcc aatcaatgaa tttataggaa taattaggca agaaggtaaa
    29281 tttcataatg aaccttcttt ttttattgga aaaattaaaa gtaaattacc agatttaaaa
    29341 atagagataa ataacatcat attagaaaaa gaagatattt tgatagatag ttggatgctt
    29401 gatagacaga tagagttatt tgatacagaa acaagtcaag aacataaaca tgaaataaaa
    29461 aatcccttta tagatacttt tgaatctgga aacacagtaa taatgtttaa aataggtgaa
    29521 aaatttgctg ttgtaagtaa attggtgagt ctagatgaat aatactatat tccctttcat
    29581 gggtgttcca gaagattata tttctccaga caatgaagaa ttacctattt ttaaagaatt
    29641 tgcttgggat tttaataaag atgaaaagat aattgaaaat ggagacttta aaatagttga
    29701 aagaaatgaa gcaattaaag tatggattta caaaacaata aaaacagata agtatgttca
    29761 tttaatatat gattggagtt atggaacaga tataaaaaat ctaatagggc aaaagtatac
    29821 taaaggtctt actgaaagtg aggcaaaaag gtatatacaa gaagctctat taactaatcc
    29881 atatatatta gaagtcaata taacaaatgc agaatttgaa gatgataatt tatctataag
    29941 tttaaatgta aaaacaattt atggagagga ggagattact tttgtataat ggccaatcat
    30001 tcagtacctt aaataataga actttaaata acataaatct ccctctttat aaaggccaag
    30061 gatcttctct ttataatata gtttctccaa taaattcaga acttgcacaa ttatatatag
    30121 aactttctta tatacataag agagttttta ttcaagataa ttttgacgat tttcttgata
    30181 gaagagtcaa tgaatttggc gtatatagaa aattaggtac agaggcaata ggtgaggtaa
    30241 catttgaggg caaaattgga actcaaatac caaatggaac aataatatct catagtgatt
    30301 tactatttgt aattattaaa gatattacaa tagatgaaaa tagtaaatta aatgtaagcc
    30361 ctatacaggc tttagagatt ggcattaagt ataatttatc agctaacaca gaatttaaac
    30421 ttatagaaga aataactgga gtaactaaaa tttataatga acttgaccta aagggtggaa
    30481 cagaaataga aactgatgaa gaattaaaag aaaggttcta taaaatacaa aaaaatcaag
    30541 cgacaagtgg aaataaggcg cattaccaat cttgggcttt agaggttgaa ggtgtttata
    30601 atgcaaaagt aattcctcgt tgggatggtc caggtacaat taaaattcta atttatggtc
    30661 aaaacaatca atcagttgac aatgaagtat tgcaaagatg tactgaacat atagaagaag
    30721 aaaaacctat tggacctact gttacaattg ttacaccaag tacttttgat attaatatta
    30781 gtgcaacttt aacacttgaa aatggatatg atattgaatc tataaaagtt gtgtttttag
    30841 atattataaa ttcttatttg atagaaaatt caagagagat tgtttatata aaagtaatga
    30901 gtttgttagc aagcatagaa ggtattcatg atataaaaaa tctccttgta aatggtgatg
    30961 ttaaaaacat aatagttgat gaagaaaaag ttcctgctgt ttcaagtgct atatttgata
    31021 tcgaggtgag ttaaattgaa gttaattgat tacctgccaa gttttgcaaa caatgaaatt
    31081 gacatacaaa tccaagaagc acttgaaaat gaacttttaa cgctaattga tgaaaaagat
    31141 gatttattag aacagttttt tatagataca gcaacttggg gacttgatga ttgggagaac
    31201 cttttaagca taaaagtaaa ttataagtta gacttcgata ctagaagaag caatataaaa
    31261 gcaaagatga gaggtaaggg tacaacaaca atagaggtta taaaagctat ctcggaagct
    31321 tacacaaaga ccaatgttga tgtagaagta tttagtaatc tatttagttt tacacttagt
    31381 tttataacaa ataattgtag ttataacact attttagaat tagataagaa aatagaagaa
    31441 ataaaacctg cacaccttga acacaaattc gagaggatat tatttaataa aaacgagctt
    31501 tatacaggtg cagcaattag tacaggagaa acagttacaa tatatcctta tgtacctaga
    31561 aatttagaaa gttttggaga aatagctatt tgtagtggaa atgatagagc attagaaaaa
    31621 gtaacattgt atcctaaaaa atagaaatga ggtgataaaa ttgacagaac aacaatattt
    31681 tactctagta actgacattg gtaaggcagc aatagcaaat gcaagtatta caggtgaaaa
    31741 agtagatttt gcaaagataa aagttggaga tggaggaggg agttcttata ctccaaatga
    31801 gagtcagaca gcactaaaaa atgtggtttg ggaaagtaca cttgaacatg cacaaggaga
    31861 taaagataat cctaactggg tagtaataca aaaattcata cctggtgatg ttggaggatt
    31921 tgaaataaga gaagttggtc tatttgattc taaagaccaa ttattagcga tttctagtta
    31981 cccaacaaca tataaaccta ctgcggattc agggactgta aaagaactat taataaaagt
    32041 aatattagtt gtatctaatg tagctaatat taatttgaaa gtagacccaa ccgtaatttt
    32101 agcaacttta aaagatatac aagaattaga cactaaaata gatacaacta aaacagaatt
    32161 aacaagcaac atagaaactg ctaaaacaga gttgaacact aaaataggag atacaacaca
    32221 acttactaca acagataaaa caaatatcgt tagtgcatta aatgaggtaa aaactagtgt
    32281 agatagtata gaaacaacag cagataaaac aagtataaaa gatacagata atttatttga
    32341 gagcgacaac gtagagggag cattaaaaga agtgatgcaa gaagtaaaag gtaatagaag
    32401 cagtattata tcaacaataa atagtaattt aataccaatg tagaaagtga ggtgataaag
    32461 tgaagatatg gaaatacaat agaactgtga agaaaagaag aggtgtctat acagaattag
    32521 atatacttgc acaaaggtat gaatttgaag cagatgggaa actttataca gcttattttc
    32581 atttatatac taataagaat atagatatat tcagagataa agatttatat aaagaatctt
    32641 atatgatgag ctatagagtt ggaaatgtaa caactgatat tgatgatgta gtacagcaaa
    32701 caactacagt aagatttata cctgaccctg ttattgatat tgtgattgat aacattagca
    32761 gaggatgtga aataaactac agaattatag ataatgaacc aagtgtaaaa ttcattgtta
    32821 cagaaaagtt aaatggcaca ataatagaaa caaagacaaa ttctagtgat aataattata
    32881 aacttaaatt aactgatgaa caaatagtac cattagcatt caattcccaa aattcaatag
    32941 taatagaaat aagtacagaa gatggtggat tagtaacaag taaaacagta acatttacaa
    33001 gaacaaataa caagccaaca attttagtta attcatatag ttctaattct gcaaaattta
    33061 cagcatctga tttagataat aatttaagta aaattgagtg gtatttagat gatgtattaa
    33121 aagaaacaat aacaacagat ttaacagcag aaaaaaccat caactatgaa tttacagaca
    33181 atgcaataca cacattaaaa atagttgcta cagatgcaga aaatgcaaca gttgagaagg
    33241 ttttaagtat aagtaaagag ataatgccac ttcaatctga tgctagttta agtgatatat
    33301 caactaagct gatagagatt ggagaagggt ttagaaatgg taaaacaagt attataaaca
    33361 ctttagcact taaaaatata gaagcaagtt taaataatac acttgtggag ttatcagaga
    33421 aaataaaaac aagttttgat agttcagacg ctagtgtgca ggatttgcaa aatagaataa
    33481 cagaattgaa taatcagctt agtcgaagaa aaaaatgggc tacaggtaga tacacattta
    33541 ctagagaaga agatactatc aatttcagaa tggttgatgg agatagtctt tccgcagtaa
    33601 aaaaactaat tatacccatt aatttagatt ttgttccaag tgttattata atagacaaaa
    33661 tgtttttagg tagttacgta cctgcacaag atgtgcaatt taattgtgtt tcaaacttag
    33721 ccgattttac acttggacgt cgtttctata ataaacaatg gatttgcact tcttctataa
    33781 aactagattc agttacacaa aatagtttta ctctttcttt acaaaaagtt gttccatttg
    33841 ataaagttag tagtacgatt ttttctatta ttaatactgg ttttgcttgg agagcatatg
    33901 aataaaaata taaattagag gtgattaaat gaatagaaat aatagaataa tttacgacca
    33961 aacaggcaag atatggcttc aaactggtga agcaatagga gatatacaag agttgtcaga
    34021 aataactgaa ttaaatttca tagatgttga atttggtagt atagactata gtaaacaata
    34081 tatagagtct ataaatccag ttacaaaaga attaaatata aaaaatatag aagttgtttt
    34141 gactgatgaa caaaagagat tagaagtatt agaaaaagaa ctaaatatgt taaaagaaga
    34201 aaataaaaat agagattctg agatagtaaa tacagcattt gaagtaggaa atatcaaatt
    34261 aaacaataat ttataggagg aaattatatg tataacttat taaagttaat gatagaacaa
    34321 aaaaactata gcactaagga ggatttacaa cataagatgg atgtattcta tgcagtaaat
    34381 aggattacag aagaacaata tttagagtta acaggtttat taaataaaga agaaacacca
    34441 gtagaaccaa caatataggt tcttttttat tgaaagaagg tgactaaatg acttttaaag
    34501 aattagttag taaagttaga aatcttgtat tagaagcaaa gaatgtaact atagaagata
    34561 cagagaataa attcactagt gaaaatgtcg agggagcatt gaaagagtgt atagatagag
    34621 cagatttggc ttttcaaagt gccgatagtg gaaaagagtt aattgcaact gctatcggct
    34681 ctcctgctac atccgaacaa acatttcaag agtatgctaa ttatattgca ggatttaaaa
    34741 atactataag tcagttaaat tctaaatata aatatactag tggaactttt acctattata
    34801 ctggtgcata tcctaatggg ctaaatatag gttttagacc agatattatt tcctatactg
    34861 ccattaatgc tgtagaggaa aaatattatt gtgtatacac acctttattc tatctttttt
    34921 ataattatac aactggagaa aaatatcttg atgttaatca taatcgttat ggaataactt
    34981 ttactgacaa tggatggata cctagaaatc cagttaacaa tggtgtgtat agttatatag
    35041 cagctaagtg ccgttagttg tcaaagactt agataaattt ctaagtctta ttttaataca
    35101 aaattaggag gaagtatgaa cgtaacaata gtttttttag caacaaatat atttataaaa
    35161 ttagtaatat tagcaatagc atttgataca ttgctaggtt gtctaagagc aataaaaaca
    35221 catcagttta atagctcttt tggaataaat ggaggaataa gaaaagttgc aatgatagca
    35281 tgtatatttt ttctagcagt agttgacata cttacaaagt ttaacttttt atttatgttg
    35341 ccacaagatt gggttgattt cttacgacta aatcatctag gaatatctga atttttctct
    35401 attttattca ttctatatga aagtgtaagt atattaaaaa atatgtattt atgtggatta
    35461 ccagttccta aacgattaaa agagaaaata ggtagtttat tagatactat gacagatgaa
    35521 ttaaatgtta aaggaggaaa ataaataatg aaaatatgta ttacagtagg acacagtatt
    35581 ttaaaaagtg gagcatgtac ttctgctgat ggagtagtta acgagtatca atacaataaa
    35641 tctcttgcac cagtattagc agatatattt agaaaagaag ggcataaggt agatgtaata
    35701 atatgcccag aaaagcagtt taaaactaag agtgaggaaa agtcttataa aatacctaga
    35761 gttaatgctg gaggatatga cttactcata gaactacatt taaatgcaag tgataggcaa
    35821 ggaaaaggct cagaggttct atattacagt aataaaggtc tagagtatgc aactagaata
    35881 tgtaagaaac taggtacagt atttaaaaat agaggagcta aattagataa aagtttatat
    35941 attttaaaca gttctaaacc cactgcaata ctaattgaaa gtttcttttg tgataacaag
    36001 gaggattatg agaaggctaa gaaacttgga tatgaaggta tggctaagtt aattgtagaa
    36061 ggtgtattaa ataagagtat tgaaagtaat gaggtgaagc aaatgtataa acacacaata
    36121 gtgtacagtg gtgatgatag agtatcagca gatattttag gattatatta taagagagca
    36181 aaagaaagtt atctagttat agatataaaa gactataaac cacatagaac acaaaatcta
    36241 tatgtaattg gtggagtaac ttgtaataaa atgaaagaaa tgagtaagac tacaggagaa
    36301 aaatttactc aactatatgg taatgatgta tggtcaacaa tggataaagt tatagaattt
    36361 gtaaaagaaa aattataaat taaaagacaa cctttaaaaa ttaattgtta ataaaactaa
    36421 taaaattttc aactcagaag gagcattata tgttaacatt tttagatttg tttgctggca
    36481 ttggaggttt taggctagca atggaaagag caggacacaa atgcgtaggc cattgcgaat
    36541 acgataaatt tgcaaactta agttacaaag caatgcataa cccaaaggag gatgaatggt
    36601 ttgaaaaaga cattagagaa attagagcag aaaatattcc caaagcagat gtgtggtgtt
    36661 ttggattccc atgtcaagac attagcattg cagggaacaa acttggattc aaaggaaatc
    36721 gttcaagttt atttttctca gttacaaaac ttattagaga acttaaagaa gaagatagac
    36781 ccaagtactt acttattgag aacgttaaaa atctacttag tgttaataga ggatttgact
    36841 tcgccaaact tctcattgag ttggatgaaa tcggctacaa tgcagaatgg caaaatctca
    36901 attctaaaaa cttcagagtt ccccaaaaca gagacagaat atacattgtt ggacatctta
    36961 gaagaagaag tacaagagaa gtatttccta tcacaggaac gagtagaaaa actactcttg
    37021 agcaattaaa taatcctaat cacagttcaa acaggattta taattcaaat ggattatcaa
    37081 agtgtttatt aagtaaaggt gggaacaata caggtcttta tagagttggc aatatcaatc
    37141 ctagtggaaa tggtgcaggc ggacaagtgt atagtagcga aggcttttct cccacactta
    37201 caactggaaa aggtgaaagc ccaaagatat taattaaaga gaatactaaa aaaggataca
    37261 tcaaagcatc tataggagat gggataagac tagatcatat agaaggtaaa actggccgtg
    37321 gaagagttca gcacaaaaaa agttgtactt tgactactgc aagtaatgct ggcgtaattg
    37381 gtaaaaaatt tagagtaagg aggttgactc caaaagaatg ttttaggtta caaggatttc
    37441 cagacgatta ttacgataga gcggcaagtg tctgttcaga aactcaacta tacaagcaag
    37501 caggcaatag tgttactgta gatgttgttg aagaaatagc aaagaaatta aaatagaata
    37561 ttaatacaat gcaaaatgtg gtaggttttt ccttccgcat tttgcattgc attagctttt
    37621 ttggaataac ttacctgtac tatcttaaat aaaaaaatgc tactatatta taaacattta
    37681 caaggagtgc ataatgttaa tacaaagctt aaaacgtggc gaattaattg taatgaaaat
    37741 cttttagaaa agaaattcaa gaatatcaaa gaaagaaatt attcgcattt gcaatgaaaa
    37801 acacaattgg cggaaatctg cgacaaaaac tctacttaaa agacttgtta aaaaaagagt
    37861 tctacttaag agaattaaaa ttttccacat atattacata cctatagtta cagaaaaaga
    37921 atatttcaaa tataaaataa atgatttaga gccagattat ttagatgaat attttatacg
    37981 attaatgtcc actgtacata aatttaaaaa tctagatgaa gaagattaaa tatctccctt
    38041 catctactct tcacatattt taatagactt tcaagcaata attgacttaa caaatcatgc
    38101 ttatcataat gtttattctc atctgcaaat ttattaaaca tttcccacac tgtattattt
    38161 attctaactg tagttctttt tatagattga tctggaagat ctatagatat atcattttct
    38221 ttaatgacac atatatgata actatcatct ttagttttaa accattccag cacattcttt
    38281 atattatcaa tttcactaga taaatattta atatcgtcat caatcttact gctaatgcta
    38341 tctatatgat atttatcatc ataactttta ttatcttcta caaacttgtt tccagttctt
    38401 ttatatcctt tcttattaag ccacttcctt atagtacttt catttgcccc taattcatct
    38461 ccaacttttt taagactttc aaattcactt aatttattat taatacaatc aacaacttca
    38521 ttaatatttt tttctttcaa atcactccaa gtcatataaa tcctcctcaa aaaaataata
    38581 gattacaaat atcatatatt tgtaatctat tatacatact ataaactaaa tcctttttta
    38641 tttcatcatt tttttgtatc cttttgcatt tgcaaaaaca tgtttatcta catcactcat
    38701 aactattatg ccaggtatat tctttttaaa tgcttcacct aacaatttag cgcctccacc
    38761 cataacaaca accgcagctg tactaactgg ataatttaat ttcaattcat tgtatatttc
    38821 tttaaataga gaaatagatg catctatatt tttctttata tcttgttttt caccatttag
    38881 gtaaaatcct ttatttatgt agtcttgtat tttttctctt tttatctcaa ctttacctaa
    38941 aaatctttcg tccatctcaa gagatttctt tattgcatca tatatattta ttgttcccac
    39001 atttatagaa tatggatcag tcgctttatt cccttgtaca agcgcaatat ctgttgtctt
    39061 tccaccaata tcaactaaaa caacatctct tcctgcaatt tggtcaacta tgtcttttgc
    39121 ataatataag tatcctgtaa caccttctgg aaatgtttga atagatctta ttgttatatc
    39181 tcttttagta gtctcatttc ctcttgtaaa ctcaacatga tattcttttc cttgtaattt
    39241 ttcaataaga ttttttctta atccggcaaa ttgtttcact ggcagaccta gccctagatc
    39301 aacaatttca tcatctgtat ttctacatat agcccctaat agtagtggta agaagttttc
    39361 tttctcatat ttaactatat ttatttcctc gtcaccttca cctaggtaat atgttttatt
    39421 gcctatttta atgctatctg aatctgaacc aaagtttttt acctctgtaa ctttactttc
    39481 aaaaatatca tctgtacttg ttttaactgc ataatttcca atatctatac ctaatttact
    39541 catttatgtt atttccccct tattattcta aacttccaaa atcaaaattg ttagctggat
    39601 cttctatttc ttttttctgt tcaactacag cattatcctt tttttcttgc attttttccc
    39661 aaacaagctc ttttaagtaa gctgtagctg ataatttctc atctaaaaac ttttcaattg
    39721 ctttttcttt atcattgttt ttaaatgtta gaactatcct actcattcaa ccacttcctt
    39781 ttttataaaa tattataaaa tttattaata tttatatata ttttactaaa ttttacacag
    39841 taattcaata gatttataag atatttttta taatatttta taatttttta cgtaatttta
    39901 taatttttta taaaatttat cattttgtat ataattaaaa acacactttt tataattttg
    39961 cataaaaaaa gagtgtttca tatatttcta aacactcttt tctacttttt ataattatct
    40021 aattgtaaaa gaagcttcag ctggactaga gaataatata atacctcctg tacacacacc
    40081 ttttccttta aatttagcaa tatatgaacc cttttttagc cctttaaaag ttactacacc
    40141 agttttagta gttccagcta atctaaagcc tacatactga gatgatacaa ttttgccagc
    40201 cttatataca tctatatttc cttcaaatcc taaaatattt ccaagcgtag atgttactct
    40261 ccaagataac tgcccaccac tcattgcagt taaattagca gtaccacctg gaacaacaac
    40321 agatttagtc gaaggcgaat ctgagtttgg ttggccaatt aattcaactg tttgtgaatt
    40381 tccagtacct tcataatcat ttccatcagc aaaagctgta aatggaatca ttactgcgca
    40441 aagcgttagt aataaagcaa ttgttttttt catttgtttt ccccctattt ttttatatta
    40501 ttctcaatta cagtttcctt tctttacgtg ccttaataat catattgtaa ttatgctcag
    40561 cagtttcaaa tgctcctacc aaaggagtta taattacact ttctattaag cacaattcat
    40621 catagtttaa actagcttct ccagcataga aattatttaa cttatctaaa tctattctag
    40681 ttaaaacgga aactgattcc attgagaatt tgtaaacatc aataagttta tataatccac
    40741 ctatatagtt ttttgctact tcatggctct taaaaaattc atcttttcct gtatgtaggt
    40801 ttacttccat aattccctcc attgttctta tatttttaac cttcaaataa tatttacttc
    40861 ttcttcaaaa cagaatattc tatcacggta gaatattctg tttttttcat tctatcatga
    40921 tagaataata ttttcaatca ttttttctat tctattttat ttaaatagaa tagaaaaaaa
    40981 gaacacttaa tatttttcaa gctaatatat taagtgttct ttttatattt ttacaagatt
    41041 atataaaatt attctttaat caattaaagt gtttttctat ttcttaataa taagaaatga
    41101 tataaaattg tatcatttta aagattttgt gatataataa aagcaagaag aactacaatc
    41161 tatttaggcg tagagtggag ttcataataa acgtagttta ttttttgaat ttaaatataa
    41221 atttaaattc aacctgtaag tcactctttg cacgagagtg gctttttgct tttttgaata
    41281 gttcactgat taagtaaact accacactag ctgttaaact agctaaaaca ttaaacaaaa
    41341 agttatccat gtaactcacc tcccctcgaa tcgttgggag gataatcttt tgtacatgaa
    41401 ctccactcta tagattgtat attacgctct tcttgctaaa tttaattata ccatattttg
    41461 taatatcagt taagagtttg cactacaata atccttcatt actgcttatc ttctttaatt
    41521 ttctccaatt cttatttata gtaatatcct tcttcattta ctagcccctg atcaaataaa
    41581 tttctcctaa cagtttcaag tttgacagga tctttgctat aatcaatttc tctttcttct
    41641 tttttaacat cttcatcatc ccaatattta tctaattctt ccttaaactt taaatcatct
    41701 ttttcttgaa ttataacttc acttatttta tttgaaagtg ttgatttgaa aaaatcataa
    41761 ttctttgtat ttataatgtc agcattatct ctttcaagtg ctatagctaa agatttatca
    41821 aaagcctgta tataaatatc ttttttaaaa tcaaattctt taaaatcaat cttaaaaagt
    41881 cttattgttc ctttagtaaa tgcagtttca tctggtatat aaaattctat tgtagtttca
    41941 gtttctttac tttcttcttg ttcttcataa attacagttt ccacaacatt tttatcaaaa
    42001 tattttcttt tatctaaatc tttaacagaa aactcaatag ctactacttt tctgcttttc
    42061 ttaatttcct tgaaatctat atcaaaatat tctgtagaat taagttcctg tatagcggga
    42121 acaataacac gtctttttaa atcattatat ttagaatact tatcttctaa taataataat
    42181 tcttttaaaa catccaaagt atatgtaatc ttattttttg tccttgtcca taaccttaat
    42241 aaatcataaa atctttgcgc atatgaattt cgtaaactaa aaaatatttg taaatttatt
    42301 ggagtatatc caacttgaat ataattttct attaagtcaa gtaactcagc agaagtttta
    42361 acagtatatg ttttatcttt ttcattataa atccagtgat atataaaact acattctccc
    42421 caactatcat catctttaat atatattgtt cctgttctta acttagtaaa tatcttgctt
    42481 attgcttgcg gggttgtata attgggattt ttcataagtt ctctaacttc ttcaatagtt
    42541 attgttatct ctaaattttt atttttctga aactcatata acaaccttac aaaaagtcta
    42601 ttctctattg tttgcaaatc ataccttgcc ataataaact tattagattt catcaagata
    42661 ttgtctttct ggtcttttac ttgattcatt atttttctcc taaatagtat ttacaaacta
    42721 atcaaaagtt tagtttattt attaaataat ataatatcaa aaccacttgc aaacgtcaat
    42781 tttatttttt gtcgcattta attatcaaag tgtcgtactt taattatcaa agtgtcgcat
    42841 ttaattatca aagtgtcgta ctttaattat caaagtgtcg catttaatta tcaaagtgtc
    42901 gcatttaatt atcaaagtgt cgcatttaaa accttacata atagtatttt caatgtattc
    42961 actatcgcta aatactatta aatactatta aagactattt attaaatact attaaagact
    43021 agtaaagata cactaatcta ttcttatcaa agtgtcgcat ttaaaaaata aaagttcata
    43081 taaataaatt taaaaatact gaatcatttt aattgttcgg aatatatacg aattatttga
    43141 tacttgcata ttaaaatagg ttttaaaaaa gatataagtc atctgaaacg attttaagat
    43201 gatttcttaa taatttatac agaaataaat actaaactcc ttaaaatgat gacctttcgt
    43261 ctctgcaact tagaaatatc aatcattaca gaaaccaatt ttaaacctgc aattaatggt
    43321 tttataaaaa acatagagtt gagtttcaaa aataaaaaag gtttatcgaa tttgtaattt
    43381 tgatgatcgg aaaaaagaaa ttgataaaaa taaagttgat taagtatagt tttaaggcat
    43441 ttaaaggctt gtctgacacg ttttaattgc aaacttgata ttatttacct aaaaagatac
    43501 aaatttgctt aaaaatggaa atagaaggtc aaatataaaa atagagaacc aaataaaatt
    43561 atattggctc tctatttatc ttcttcccaa tcctcaataa acttttctaa actttttaat
    43621 ttatttgcag atatttcttc atcatcatgt aatgcactta taaaactttc aaaggaattt
    43681 ctgtgaaaga aactaaagaa acttttagtt tcaactttta aatattcttc tttttctatt
    43741 aatgctttat aatatgtaaa tctatctttc ttaacaactt ctaaaaatcc tctaccaact
    43801 aaccttctta aaaaagttaa catagttgat tttttccatt catatttttc acctaatgtt
    43861 tttaaaattt cagtagatgc aacttcttca tcttcactat cccaaatgta catcattacc
    43921 aaaagttctg attctggaat cttttttatc ttcataaagc acaccacctt ttcaaaagta
    43981 taactaaaat actataacaa tagagttaac tatacaatac taaatgtcta aaaatgttat
    44041 tccaattttt accaattaat ttttctttaa aaaaaattta agcaggagta aaaaaaataa
    44101 aaaattatca aaatttatca atataaaaag aaatataagt ataaattttt ctctttgtat
    44161 attgattgta tacaattcta tttttgtaaa attaaatttt acaaaataat caaaaatggt
    44221 aaattttgac atttgcctaa tgtcaaatta ggtttacaat tgtaacataa agaagatata
    44281 tatcgaaata atacatataa aatacatata aaactacgta cgtaaaatat ttttaaaaat
    44341 aatcaaaaat cttaattttc aatattgaat acaattaaat tttcaaactc gtctagggca
    44401 tgacatacaa aagcaaaaaa cattaattta gtatctgata aaaatttaaa aattataaaa
    44461 gggggaagtc tgttattttt cagaagcaat gtaactgtaa aaatataaat cagaatattt
    44521 tgaaaatatg ggggattcta tgggagaatt aaaacttgaa tcaaaaatga attgcactaa
    44581 aaataagaaa aaagttgcta tatctataca tcaaatgaaa aaaatttcta aaaaagatta
    44641 tgttttttta acagattgca tagaaacatt ttatatgaaa ataaaagagg tttgataaat
    44701 tttcaaacct ctttttattg ctatttgttt ttcttttgca ataattctat cattttcaag
    44761 atagcatctc tatcttcttg ttctaattca taaagtttgt tagttatttc ttttacatca
    44821 tcatcaattt ctaagtcagc taaacaatct ttataaattt caccttttcc acttcttagc
    44881 cattcctcat ttacttcaaa ttctctgcat atgtcgttaa tacttctttc tgtcaaaact
    44941 ctttttcctt tttccaataa agaaatttga tcttgtgata agaaaattct ttccccaaac
    45001 gctctctgcg aaagtctttc aagagttctt aactcattta atctttttcc aatttcttta
    45061 gcttttaatt tttttgaaac ttctgccatt tttacaatcc tcctctgtca ttactatact
    45121 ataaaaacac gactttgtca ttaaaaaaat aattataacg acaaaatcat gataaaaata
    45181 aaaaagatat tgatttttaa cgacaaagta gttatactta aaataagttg aacgacaaag
    45241 taagtgtagg agggtgaaaa atgatttgtt caaacaaaga aaaagtagaa acagctttga
    45301 tgatagatta ttt
//
LOCUS       DAELTA010000012        40402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.012, whole genome shotgun sequence.
ACCESSION   DAELTA010000012 DAELTA010000000
VERSION     DAELTA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.012"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            196..336
                     /locus_tag="KPG13_000590"
     CDS             196..336
                     /locus_tag="KPG13_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984334.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(511..831)
                     /locus_tag="KPG13_000591"
     CDS             complement(511..831)
                     /locus_tag="KPG13_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG0984335.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(951..1688)
                     /locus_tag="KPG13_000592"
     CDS             complement(951..1688)
                     /locus_tag="KPG13_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG0984336.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1903..3726
                     /gene="acd"
                     /locus_tag="KPG13_000593"
     CDS             1903..3726
                     /gene="acd"
                     /locus_tag="KPG13_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG0984337.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3941..8239
                     /locus_tag="KPG13_000594"
     CDS             3941..8239
                     /locus_tag="KPG13_000594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG0984338.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8443..8904
                     /locus_tag="KPG13_000595"
     CDS             8443..8904
                     /locus_tag="KPG13_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG0984339.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8934..10088
                     /gene="nusA"
                     /locus_tag="KPG13_000596"
     CDS             8934..10088
                     /gene="nusA"
                     /locus_tag="KPG13_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG0984340.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10095..10376
                     /locus_tag="KPG13_000597"
     CDS             10095..10376
                     /locus_tag="KPG13_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG0984341.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10366..10677
                     /locus_tag="KPG13_000598"
     CDS             10366..10677
                     /locus_tag="KPG13_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG0984342.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10699..12639
                     /gene="infB"
                     /locus_tag="KPG13_000599"
     CDS             10699..12639
                     /gene="infB"
                     /locus_tag="KPG13_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG0984343.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12682..13062
                     /gene="rbfA"
                     /locus_tag="KPG13_000600"
     CDS             12682..13062
                     /gene="rbfA"
                     /locus_tag="KPG13_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG0984344.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13043..14026
                     /locus_tag="KPG13_000601"
     CDS             13043..14026
                     /locus_tag="KPG13_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG0984345.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14039..14944
                     /gene="truB"
                     /locus_tag="KPG13_000602"
     CDS             14039..14944
                     /gene="truB"
                     /locus_tag="KPG13_000602"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG0984346.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15075..16004
                     /locus_tag="KPG13_000603"
     CDS             15075..16004
                     /locus_tag="KPG13_000603"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG0984347.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16108..16365
                     /gene="rpsO"
                     /locus_tag="KPG13_000604"
     CDS             16108..16365
                     /gene="rpsO"
                     /locus_tag="KPG13_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG0984348.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16605..17450
                     /locus_tag="KPG13_000605"
     CDS             16605..17450
                     /locus_tag="KPG13_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0984349.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17575..19686
                     /gene="pnp"
                     /locus_tag="KPG13_000606"
     CDS             17575..19686
                     /gene="pnp"
                     /locus_tag="KPG13_000606"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG0984350.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19952..20695
                     /locus_tag="KPG13_000607"
     CDS             19952..20695
                     /locus_tag="KPG13_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0984351.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            20853..22100
                     /locus_tag="KPG13_000608"
     CDS             20853..22100
                     /locus_tag="KPG13_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0984352.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22135..22380
                     /locus_tag="KPG13_000609"
     CDS             22135..22380
                     /locus_tag="KPG13_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0984353.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22390..23613
                     /gene="dapG"
                     /locus_tag="KPG13_000610"
     CDS             22390..23613
                     /gene="dapG"
                     /locus_tag="KPG13_000610"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0984354.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23726..24460
                     /locus_tag="KPG13_000611"
     CDS             23726..24460
                     /locus_tag="KPG13_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG0984355.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24583..26994
                     /locus_tag="KPG13_000612"
     CDS             24583..26994
                     /locus_tag="KPG13_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG0984356.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26997..28055
                     /gene="mnmH"
                     /locus_tag="KPG13_000613"
     CDS             26997..28055
                     /gene="mnmH"
                     /locus_tag="KPG13_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG0984357.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28049..29383
                     /gene="rimO"
                     /locus_tag="KPG13_000614"
     CDS             28049..29383
                     /gene="rimO"
                     /locus_tag="KPG13_000614"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG0984358.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29370..29912
                     /gene="pgsA"
                     /locus_tag="KPG13_000615"
     CDS             29370..29912
                     /gene="pgsA"
                     /locus_tag="KPG13_000615"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG0984359.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            30200..31246
                     /gene="recA"
                     /locus_tag="KPG13_000616"
     CDS             30200..31246
                     /gene="recA"
                     /locus_tag="KPG13_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG0984360.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            31422..32963
                     /gene="rny"
                     /locus_tag="KPG13_000617"
     CDS             31422..32963
                     /gene="rny"
                     /locus_tag="KPG13_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG0984361.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            33277..35613
                     /locus_tag="KPG13_000618"
     CDS             33277..35613
                     /locus_tag="KPG13_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0984362.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            35995..37776
                     /locus_tag="KPG13_000619"
     CDS             35995..37776
                     /locus_tag="KPG13_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG0984363.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(37858..38388)
                     /gene="lepB"
                     /locus_tag="KPG13_000620"
     CDS             complement(37858..38388)
                     /gene="lepB"
                     /locus_tag="KPG13_000620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0984364.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(38428..39252)
                     /locus_tag="KPG13_000621"
     CDS             complement(38428..39252)
                     /locus_tag="KPG13_000621"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0984365.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            39509..>40402
                     /locus_tag="KPG13_000622"
     CDS             39509..>40402
                     /locus_tag="KPG13_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0984366.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLD"
BASE COUNT        16279 a         4375 c         7393 g        12355 t
ORIGIN      
        1 aaaaaagata ataatagtta gattttttat atgaatttta ataggtaaaa tatcctattg
       61 ttttgtagat atatcctaat tatgtcaaaa aataaaaaaa tttttattta ttagagtttt
      121 tggaaataat aataacagat attataatta attaacagtt tttgtaagta tttgacaata
      181 gagagggtgt aacaaatggg aataatagag tcagcatcaa aattggctga gatggttcat
      241 ttattggctg ttgaaaaggg gataactgat atagaagcat gggatgaagc tgtgaaagaa
      301 tacagcaaaa tttatgaaga aagaagaaat gaatagaatg ataaaaaatg aaaacttgat
      361 gataaaaaat aactttattt aaagaaattc aatttttata gaagtattaa aaataatata
      421 aataaaaaga tggtatttca aatgtaagta aaaaatcatt ttgggatacc atccttatta
      481 tttattttaa taatatataa gtaataaatc ttacttatcc atatttttta tgtaacctct
      541 tttttctaac ttttttatat gctccttacg tatttcatca agattaatac catattgttc
      601 ttctagctta aacatcatag taacacatgt ctgagctaca tccattagtt ccttagcagt
      661 tttctttata atatcaactt catctaaatc attattttct ccacttagat ttctgaattt
      721 acctatacat tcagctaatt caccttgttc ttcacccatt ttgataaatg tagattctag
      781 tgttgggtct aaattattaa gctctggtaa agatatagtt tttagtttca taaaagctcc
      841 tttcaagtta ttcgtattta tactaaaaca tcataacaaa aataatacta aaaaaaaact
      901 ttaaattcaa cataaaaact tacatatata ataaaaatcg tgaaataaat ctatatttgt
      961 cttaatacag cctcatataa aacttctcca ataggtttat ttattacaag agaagctgac
     1021 ttatcataga ctgtatttgc tttattaata agagcaatat aatcaccttt gtaataatct
     1081 ataaaacttg ctgctgggta cacagctaat gatgtacctc ctataatcaa taaatcagca
     1141 ttacttatag cagaaattgt tttagtaatg acatcactgt caagagcttc ctcataaagt
     1201 actacatctg gttttactat agaaccacag ttgtcacagt aaggaatatt acctcccaga
     1261 tttaacatag attctaagtc aaaaaactta ccacatttag tacaataatt tctatgtaca
     1321 gaaccatgaa gttctagcac atttttactt ccagccattt gatgaagtcc atcaatattt
     1381 tgagttatga cagctttaag cttacccatt tcttcgagtt ttgctaaagc tatatgagca
     1441 ttattgggtt ttgcattagg atatattaat ttatccttat aaaattcaaa aaattcttct
     1501 ggatatctaa caaaaaaagt atgtgaaact aattgttcag cagtaaattg tttattgagt
     1561 ttttggctaa ataatccagt agaacttcta aagtctggaa tattagattc agttgaaaca
     1621 cctgctccac caaaaaatac aatattattg tgattagcta ttaagtcttt taagctattt
     1681 gcatccatag ttacacctcc aaattattat atataaattg tactacatat taggtcgttt
     1741 atgttttatt aatttaaata aatatatagt ttaataagtt aaataaaggg aatataaata
     1801 aaatgtatac agagtataaa tagacaatat ttactaaaat aacaagttgt cgtataatta
     1861 tattaaaatt gttttagtgt ataagaaggg cgggggaagt acgtgaagaa aaaagcagct
     1921 ttagcaacat tggctatgtt acccttaggt gtggtaaatg cacatgctga tggagatata
     1981 ggtatagtga ctataaatta tttaaatgta agaaatgaac caactgccga aagtagcata
     2041 gcctttgttg ctaaaaaaga tgataaagtt ttgattaaag actcttctaa tggatggtat
     2101 aaaataaaag ctgaatctgg acaagaaggt tgggcttcat caaaatatat agcaaaatca
     2161 aatagtgact ctttaagaac gtctacaaat aaagaaaaac aagttatttc aaatagttta
     2221 aatatgagaa atggagcagg tacaagttac agagtaataa ctgtactaaa aaaaggtcaa
     2281 aaagtagaag taatatcaga gagtaatgga tggtctaaaa tcaaatatga tggaagacta
     2341 ggatatgtat ctagctctta tttaggagac gtttcaaact caactaataa atcaaaaact
     2401 aaacaagtga atacaacttc actaaatgta aggagtggac caaatacaag ttatggtttg
     2461 ttaggcaagt tgccaaaagg aagtaaagta gaagtaatat cagaaggtaa tggatggtcc
     2521 aaaataaagt ataatggaaa agatgcctat gtatctagta tgtacttatc ggatgtaagt
     2581 caaagtaatt cagacaattc tagtcaaagc aatgataaaa agaatactga taaggtcgta
     2641 aatacagctt ctttaaatgt aagaagtgga ccaggttcta catatagtaa gttgggaaaa
     2701 gtttataaag gaagtaaagt aactgtacta tcagaaagta gtggatgggc taagattaat
     2761 tttaacaata aagaagcatt tgtagtaggc aattatttat ctacttcagc agatacttca
     2821 aataataact caaatagtaa ctctgataac agttctaata gtaatggcaa taattcttca
     2881 tcatcaggtc aagtaaatgg tatgtcaggt ataagtgggg ctaaaattga ttataaatct
     2941 ttgagttata ctttagaatc tcatataagt aaacaagttg agaaagcagc atcaggagga
     3001 aatgtgatag ctccaagtaa taggaaaagc actccaagtc ctgaatttag tactttttca
     3061 gctcaaagaa caagctcatt tgtaaatgca agttcaagtg atatagaata ttatttaaat
     3121 cctaaaaatt ttacaaatac tactaaaggt atgatgcagt ttttaaagat taacagttat
     3181 agagatggta tttcagaatc aagtcttaac tcgtatctta atggtttatc ttctagtgta
     3241 tttaaaaacc agggagcagc ctttataaat gctgccaaga agtataatat agatgttgta
     3301 tatctagtat ctcatgctat gtgggaaact gcttatggta agtctacact tgcccaaggt
     3361 caaacattaa catcttataa gggacaacct cttagtaagc cagttaaagt atacaatttt
     3421 tttggtatag gagcaataga taaaagtgct aatgtttcag gtgcagaagc agcgtattcc
     3481 aatggctgga ctagtgtaga agctacaata gatggctctg caaagtggat atcacaaaat
     3541 tacgtaaata gttctaagta caatcagaat actatctaca agatgaagtg gaattatgat
     3601 tatacatggc atcagtatgc aactgatgtg aactgggcta atggaatttc tggaattatg
     3661 gaaaatttaa ttggtcttta tggaggagga agtagtttgg tttttgaagt tcctcaatac
     3721 aagtaataca taaataaaaa atatagacta tgggtagttt tcattttatt taaattatct
     3781 atagtctttt tgtatataat ttataatctg taaatttaat gctaacaaaa cttttatgtt
     3841 ataatattaa ttctaagtaa atataagtga gtaataggta gaaatagata aaaagtggta
     3901 ataatatata taaagataat tttgagaaag gaggatatat atggaaagta ttaaggaata
     3961 tttggataag ttagaaatta ataatagtgg tcttggaaag caacttaaag aagtttatat
     4021 aaatagagta gtttatttta aggaagataa gatagtttat ttttatctaa cttctaaaga
     4081 tattgtttct catgaacttt tagataaatt taaagaagaa ttgatgtata aactcgatta
     4141 ttttaaagat atgaggatga agataagatt tactggtctt gaaagaaaat ccaataaaga
     4201 tgttataaaa aaatattgga ataatatatt gtatatatta aagtatcttt gtccatctat
     4261 tgcagggtgg tataaacaag tagaatttct ttgtttagaa gaagaattaa aaataaaact
     4321 tccaaaaggt atattttatg agcgattaat gaaaaaaaat gtaacttatg tacttaaaac
     4381 cgtgcttagc gaagagttgg gattagattt aaatataaca atagaaaaag ctgttgatga
     4441 aaaggtaaat aaagaaagac tcataagaat caatgataga gaaatggaag aaaaaattag
     4501 ggctttagaa attggaaaag ttaataactg tgaagaaaat gaagaggaaa gttatgtcat
     4561 taagtctgat gtagatgaaa atttaatcta tggagataat gcaaatgcta tgattgaaaa
     4621 tatagttgaa ttaaatgcat cttctggaac agttgcagtt gttggagata tatttgatgt
     4681 tgacactaaa gaacttaaaa acggaaaaat acttatgata gcttcaatta cagattatac
     4741 tagctcaata agctgtaaac tttttttaac agatacaaat aaggatggag tacttgaaaa
     4801 tgttaaaaaa ggagcatact taaaaatcaa gggtgacata gtttatgata catatcaaag
     4861 agaaattagt atgatgatta gtgggataag aaaagaaact agaatagaga gagaagataa
     4921 atcagaggaa aaaagagtag aactccatgc acatacacaa atgtcttcta tggatgctat
     4981 ttgttctgtt aaaaagcttg tagagcgtgc agccaaatgg ggtcattctg ctattgctat
     5041 aacagaccat ggtgttgtac aaggatttcc agatgctatg aatgcaggta agtcaaataa
     5101 tataaaaata ctttatggag tagaagggta cttggttgaa gatgattctc tcattataga
     5161 ggatgcaaat gataaagaac tttcgcagac atttgttgta tttgacatag agacaactgg
     5221 tttttctaat acaaatgata aaataactga gattggtgct gtaaaaatag aaaactttaa
     5281 gattgtagat agatttagtg agttaataaa tcctgaaaaa gatatttctt ataagataca
     5341 ggaattgaca ggaataacta atgaattaat taaagacaag ccaaccattg aagaagttct
     5401 tcctaagttt atggaatttg ttggagatag tgtattagta gcacataatg cagagtttga
     5461 tactggattt atatcgcaaa aatgtaggga acaaggcttg ttatataata acaaaaaagt
     5521 agatacactt atgttagcaa gagtaatgtt gccaaactta aaaagacata gattaaatgt
     5581 cgttgcaaaa gaacttggta ttcctctttt aaatcaccat agagcagtgg atgatgctga
     5641 agctacagca cttatattta ataagttttt gcagatgctt acagaaaaag gtgcaaaaac
     5701 tctaagtgat gtaaacaata tacttggaaa gattgattac acaaagctta gcacaaatca
     5761 tataaccttg atagcaaaaa attctatagg tataaaaaat ttatataaaa taatttcaga
     5821 tgcacatgta aatcattttt ttagagcacc aagaatcctt agaagtgtat taaatgaata
     5881 taaagaagga ttaataattg gttcggcttg tgaagctggg gtggtttttc aagctgtaaa
     5941 gaaaaacgtg tctgatgagg aaatgaaaaa aataatagat ttatacgatt atattgaagt
     6001 aatgcctata gataataata gatttatgat tgataaagga gaagtaaagg acgaagaaga
     6061 gttaagggag ttgaatagga aattaattga tacagccaaa aaatttgaca aaattccagt
     6121 agctactggg gatgttcatt ttttagacaa acatgaagct gtcttaagaa aagttcttaa
     6181 atattctcaa ggttttaaag tagatgaaga agaaacttat ttacatttta gaacaacaga
     6241 tgaaatgttg gaagagttta gttatcttgg agaagaattg gcatatgaag ttgtagttga
     6301 aaatagtaat ctgattgccg atatggttga agatataaaa cctataccag atgatactta
     6361 tcctccaatt atagagggct cagatgtgga gttaagagaa atgtgttaca aaaaagccaa
     6421 acgaatctat ggagacccaa taccagaaat agttcaaaaa agactggata gagaattaaa
     6481 ttcaataata agcaatgggt atgcagttat gtatattata gctcaaaaac tggttgcaaa
     6541 atctctttca gatggatatt tagttggttc aagaggctca gttggctcat cttttgcagc
     6601 tactatgagt gatataacag aagttaatcc tcttccagca cattatattt gtgaaaacga
     6661 agactgtaaa tattcattct tttatgaaat aggtgaatgg ggctcaggtg ttgatttacc
     6721 agacaaggat tgtccaaagt gtggaagaaa acttgctaaa aacggtcatg atattccatt
     6781 tgaggttttc ttaggttttg aaggtgataa agaaccagat atagacttaa atttctcagg
     6841 aaattatcag ccaacaattc ataaatacac agaagaacta tttggagagg ggtatgtata
     6901 tagggctggg actataggta ctgtagcaga aaaaacagct tttggatacg caagaaaata
     6961 tgtggaagaa aataatataa gtgtaccaaa tgcagaagta cttaggcttt caaatggatg
     7021 tactggagta aaaagaacct caggacaaca tcctggtgga gttatggtta taccagacta
     7081 taaagatgta tacgatttta cacctataca gtatccagca aatgatactt cttgtggtgt
     7141 tataactact cactttgatt atcattcaat aagtggtaga atacttaaac ttgatatact
     7201 tggacacgac ggtcctacca ttataaggat gcttgaagat atgactggaa taaatataac
     7261 agaaattcct ttagatgata aagagactat gtctctattt acatctacag aagcattagg
     7321 tgttacacca gaagaaataa actgtcctat aggttgcctt gctgtaccag agtttggaac
     7381 taagtttgta cgacaaatgt tacttgacac aaaacctact acatttgcag aacttgtacg
     7441 tatatctggt ctctctcatg ggacagatgt ttgggtaaat aatgcacaag acttagttag
     7501 agaagatata gttggattaa aagatgttat atctacacga gatgacataa tgaactattt
     7561 aatatttaaa ggattacctc ccaaaatgag ctttacaata atggagagtg taagaaaagg
     7621 taaaggttta aaaccagaac acattgaaga aatgaaaaaa aataatgttc cagaatggta
     7681 tataggctca tgtaaaaaga ttaagtacat gttcccaaaa gcacatgctg ttgcttatgt
     7741 aatgacatca tttagaattg catattgtaa agttcactat ccagaagcat tttatgcgac
     7801 ttattttacg acaaaagttg aggattttga tgcagattta attgttaaag gattagatgc
     7861 tataaaatca aaaataaatg aaattgagtc actgggaaat gatgcaacca caaaagaaaa
     7921 aggtatgttg accgttttag aagttgctct tgaaatgtat gctagaggaa taaaattatt
     7981 accagttgat atatataaat cagatgcaac tgagtttata gttgtaggag aaaaaacatt
     8041 acttccacca atggcagcaa tacaaggtct tggaggaaat gcggctataa acatccaaaa
     8101 tgaaagaaaa aatggtgaat ttatatcaaa agaagatttg agaaagagaa caaaaatatc
     8161 aaaaacggta atagaaactc ttactaatca tggttcactt gagaatatga gtgagagaaa
     8221 tcaattgtca ttgttttaag ttagcccaaa aagttgcaaa taaaagaatt ttatgatatc
     8281 atagtataga aaatagtata ctttgcagaa actaagagtg agacagctgt tctcactctt
     8341 ttattttaaa tatattatta aaaagctggt ttatttttat taaatatatt ttatttaaga
     8401 ttatataaac tggtcattta caattgaata ggaggaactt taatgaaaaa aaatttagaa
     8461 gctacaattg aggagattgt aactaaaata acggatgaac atggatttga aatggttgat
     8521 gttgaatatg taaaagaagc aggagaatac tacttaagag tatacataga taaagaagaa
     8581 ggaatatctt taaatgaatg tgaattagtt agtagagaat taagtccaat actagatgaa
     8641 aaagacccta taaaagaaaa ttacttttta gaggtatctt ctccagggct tgatagagca
     8701 ttaaaaaaag acagagattt tgttagatat caaggtagag atgtagattt aaaattatat
     8761 aaaccattaa atggatgtaa gcaatttgaa ggtgaacttg taggattaac agaagataat
     8821 aatataaaaa tcattgcaaa tggtaaggaa atggaattta atagaaaaga tgttgctatt
     8881 gtcagattag caataaagtt ttagtggtat attaaatagg gaggatataa aaaatgaatc
     8941 atgaatttat ggaagcatta gatgaattag tagttgacag aggtatagat aaagaaatac
     9001 ttatagatac tatagaacaa gctcttttaa ctgcatacaa gaaaaacttt ggttcagcac
     9061 aaaatgttag agttgaattt gatagagaca agggagatat aaaagtattt tctcaaagag
     9121 ttgttgttga tgaatcagat ttatatgata ctttcttaga aatagagtta gcagatgcta
     9181 gagagataaa ccctaattat gaattaggtg atattataga acatgaagtt acacctaaaa
     9241 actttggaag aattgcagct caaacagcaa agcaagtagt tgttcaaaga ataagggaag
     9301 ctgagagaga aatagtgtat aatgaattta tagaaaaaga aaatgaaata gttacaggag
     9361 aggttgctag agcaaataaa aatgttgttt atgtaaatct tggaagaata gaaggtataa
     9421 tgactcaaag tgaacaaata ccatttgaga attataaagc aggtcaaaaa ataaaattct
     9481 atatacttga agttaaaaag actaacaaag gacctcaaat agttgtatca agaagtcatc
     9541 cgggtctagt aaagagattg tttgaagaag aagttcctga aatatatgaa ggaatagttc
     9601 aaataaaatc tgtagcaaga gaagcagggt caagaactaa aatggcagta agatctatag
     9661 atgaaaaaat agacccaatt ggagcttgtg ttggaccaaa aggacttaga gtaaaaaata
     9721 tagtggatga acttggcgat gagaaaatag atataataaa atatagtgaa gacccagcag
     9781 agtttatatc agcagcactt agcccatcaa aggttgttag agtagatata gatgaagatg
     9841 aaaagtctgc cttagtaatc gttccagatt atcagttatc gcttgctata ggtaaagaag
     9901 gtcaaaatgc aagactagca gcaaaattga ctaactggaa aatagatatt aagagcgaat
     9961 ctcaagcaga acaagaaatg ttaaatcttg aaaattccaa agaaatagaa gctgaggatg
    10021 ttgaagctga atttatcgac caagaagagt tagatacttc agaggataat tttagtgaag
    10081 aagagtaagg agggatgatt ttgaaaaaag taaaaaaagt gcctcaaaga aaatgcatag
    10141 catgtcaaga tagagactct aaaaaagagc ttataagaat agttaaaaat aaagaaggaa
    10201 aaatattttt agacccaaca ggaagagcca atggaagagg agcatatgta tgtaaaagct
    10261 ctgaatgtct aaaaaaagct ataaagagta aagcattaaa taaagctttt aaaatagatg
    10321 ttcctgatga agtctatgat aatctactta tggagttaga agaatatgaa aaataataaa
    10381 gaaaaagtac tttcgttctt agggctcgca actcgtgcag ggaaaatagt gtctggtgat
    10441 gattcaactt tgcttgacct taaaaaagga aaagtaaatt tgattttaat cgcagaagat
    10501 gcatcaaata atactaaaaa actttttaaa gataaatcaa cttttagaaa tattccatat
    10561 ctattttttt caacaaaaga ggaaatagga tttgcaatag gaaaatctcc aagagcagtt
    10621 gtaggaataa aagatgaaaa tttttctaaa aagataattg aactaataga aatttaaaat
    10681 aaaatagggg gtgatcttgt gtcaaaaaca agagtatacc aaatagcaga agaattaaat
    10741 atatcaaatg aagagcttat aaataagtta gctgaattag atataaatgt aactgacaaa
    10801 gattcagtat tagaaggtga agagttagaa ttagcacttg aaatgttagg tgaagattta
    10861 tctcaagaaa atggaaatgt tatagaaata gatggaaagt tgacagtaca agtattagca
    10921 acgaaattag ataaatctcc atcagaaata attatgaagc ttatgaaaat gggaactatg
    10981 gcaacaataa atcaagagat aagttttgag attgctgctt tggctgccaa agactatgga
    11041 tttgaattaa cagtagctga aagtgatgat acagaagctt tagaaataga agctttaatg
    11101 gaaattgaag aagataaaga agaggattta aaacctagac caccagttgt tacagttatg
    11161 ggacatgttg accatggtaa gacatcttta ctagatgcaa taagaaaaac tgatgtgatt
    11221 tcaggtgaag ctggaggaat aactcagcat ataggtgctt cagaagttaa aatcaatgga
    11281 cacaaaatag tatttttaga tactccagga catgaggctt tcacatcaat gagagcaaga
    11341 ggagcacaag ttacagatat agcaatatta gtagttgcag cagatgatgg tataatgcct
    11401 caaacagttg aagctataaa ccatgctaaa gcagcaggtg tacctttaat agttgctatt
    11461 aataaaatag ataaaccagg tgcaaaccca gataaggtta aacaggaatt agcagaccaa
    11521 ggattattag ttgaagactg gggtggagaa gtaatagcag ttccagtatc agctaagaaa
    11581 aaagaaggaa tagatacact tttagaaatg gtattattgg ttgctgaaat ggaagagtta
    11641 agagctaatc caaataaaag agcagtagga actgttatag aagctgagct tgataaagga
    11701 agagggccag ttgcaacagt acttgttcaa ggtggtactt taacagtagg tgacccaatc
    11761 gttgcaggtg ttgcatgtgg taaagttaga gcaatgataa atgcaaaagg taagagagta
    11821 aaaacagcag gaccatctac agcagtcgag atattaggtc tatcagaagt tccacaagga
    11881 ggagaccaat ttgtcgaagt tccaactgat aagattgcta gaagtgtagc agcaagaaga
    11941 caacaaatag ttagagatga aatgttaaaa tcaactcaaa gattatcact agatgctcta
    12001 tttagtcaaa tgagtgaagg ttctataaaa gaccttaata tagttataaa agcagatgta
    12061 caaggttcag tacaggcagt taaacaatct ttagaaaaat tatcaaatga agaagttcaa
    12121 gttaaagtaa tacatggtgg tgttggagct gtaacagaat cagacatatt acttgcagca
    12181 gcttctaacg caataataat aggattcaat gtaagaccag ttccaggtgc agaatctcta
    12241 ggagaaaaag aaaatgttga tataagaaca tatactataa tatacaaggc aatagaagat
    12301 atacaagcgg ctatgactgg tatgttagac cctgaatatg ttgatgaaga aacaggaaaa
    12361 gctgaaataa gagaaatata taaaatatct ggtgttggaa cagtagctgg atgttatgtg
    12421 actaatggta agatatttag aaattgtaaa gtaagattag ttagagatag cataataata
    12481 catgaaggtg agcttgcagc acttaagaga tttaaagatg atgtaaaaga agttaattct
    12541 ggatacgaat gtggtatgag ttttgtaaat tataacgaca taaaagaagg cgatatagta
    12601 gaagcttata taactaaaga agtagaaaga aaattataaa ttcgtatcaa agaaaatcag
    12661 ttagtaaaga aggtcgatgg tatggcatca tataatagaa caagaaggat agcagaagaa
    12721 ataagaaaag tcgtaagcac tatgcttata aatggagtaa aagaccctag aataacatct
    12781 atggtgagtg taactgatgt agaagttaca aatgacctaa gatatgctta tgtatatgta
    12841 agcattctag gtggagatga agaatccaca ctaacaggtc ttaaaagtgc tggaggatat
    12901 ataagaagag aagtaggaaa aaatataaaa cttagatata ttcctgaaat agtatttaaa
    12961 cttgatgatt caatagaaaa aggaatgtat atggatagcc ttataaaaag agttaatgaa
    13021 aaaaatgctc aacaaaatga ggatgaaaat tatgatgaat aatatggata gtattataaa
    13081 ctatatttat gagggtgact attttatagt tacctctcat atcagtccag atggagacaa
    13141 tattggttct acactttcaa tgtattatac attgaagaat ttaggaaaaa atgtatatta
    13201 tgtacttgat gatgaagctc cattaaattt aaggtttttg gttgaagatg taaaaatcta
    13261 taagtctaat gaatttaata tgaagaacta ttctctaatt gcacttgatt gtggggataa
    13321 gatgagaata tgtgtgagtg atgaaatcaa aaataatgca tcaaaattaa tctgtattga
    13381 ccatcatgct agtaatgatt catatggaga tttaaattat gtagatattg atgcatcttc
    13441 tacatgtgaa ttagtttata atttattagt tagatttcag caaataaaag atattaatat
    13501 tataaatgaa gatatagcaa catgtttata tacaggtctt gtaactgata ctggaaactt
    13561 ttcgtattca aatgtacatg caagtagctt tgaaatggca aaaaatttac ttgttttagg
    13621 tgcacaaaaa aatactataa tacaaaatat atatcaaagc aattcttctg gttattacaa
    13681 attattagga gaagctctaa agggtttgga gatatttgat tcaaaggtat ctagtatagt
    13741 tttaactcaa gacatgatga atagaaataa tattagcttt aatgatgttg atggaataac
    13801 aacatataca agagacataa aaggtataga agttggaatt ttatttaaag agaaaaaaca
    13861 aaatgaaatt aaagtgagtt ttaggtcaaa aaattatgtt gatgtaagtg aaatagccaa
    13921 gttatttggt ggtggtggtc atgtaagggc tgctggatgt actattagag atagtattga
    13981 taatgcaaag aaaatggtac tagaggcagt gttaaaatca atttaaggtg acagtaaaat
    14041 gaataagatt ataagtatat tgaaacccac aggtatgaca tctcatgatg ttgtaagtag
    14101 agtgagaaag attttaaata taaaaaaagt tggtcataca ggtactcttg acccagatgc
    14161 ttcaggtgtt ttaccaatct gtatagggaa agctacaaaa gtatgtgaag ttattttaaa
    14221 caaagataaa tcgtatatat gtgagcttac tttaggtata agtacagata cttatgatgc
    14281 ttcaggtgaa attttaaaaa aagttgatga ttttaaattt agtaatgaag acatagaaag
    14341 agcttttgac acacaaagag gagagataaa tcaacttcct ccaatttatt cagctttaaa
    14401 ggtaaatgga aagagaatgt gtgatttagt aagaagtggt agacaatctg aaattacttt
    14461 aaaaacaaga agagtaaata taaaagacat aaaaatacta agtataaaag gtaataaagt
    14521 aatgttttat gttgagtgtt ctaaagggac ttatgtgaga agtatttgcc atgatatagg
    14581 agaatatcta ggttgtggag ctcatatgtc gtttttgaat agaacatctt ctggtaaatt
    14641 tgacttagat aattcaataa cattagaaga attagaatta ttttatgaaa ataaaacttt
    14701 agacaaatac ttatatgata tagattatgt ccttgattcg tttaattatg ttgtattaaa
    14761 ccctaatgct ataaaatatt atagtaatgg tggctcaata gatgataaaa ggtttttaaa
    14821 gaataatttc gataaagatg atgaatttgt aagagtatac agtacagata atttcttagg
    14881 attaggaaag ttatcaaaac acaataatac aataagtgtt aaaagtgata agatgttcat
    14941 ataaccaatg tatttaaaaa aacaacaaaa gagatagtac tataataact tctatagtct
    15001 atctctttta cttatgctat aatagtttgt gaataagtta agagtaaatt atattttagc
    15061 ggggaacatt tattatggtg attataaaat ctataaaaga aatagcaaac ataaaagaaa
    15121 gtgtcattac cataggaaat tttgatggtg tacacaaagg acatcaagtt cttataggaa
    15181 aaacagttga acatgcaaaa aaagaaaata tacagagtat tgtatttaca tttgcaaatc
    15241 atcctgtaaa ttactttaga ccaaactcaa caaaaaatat tataagcaat gatgataaaa
    15301 taaaattatt agaagatttg ggaatagata tagttgtaat cataccattt gatgagtata
    15361 tgactaaaat acctgcaaag gatttcgtag atgagatact agtaaaaaaa ttaaaagcta
    15421 aaaagatagt aattggacat gactttacat ttgcaagggc aaaagaaggc aatgttaatc
    15481 tattaaaaag cttagaacat gaatttggtt ttgaggtaga agtaataaaa cccatcaaaa
    15541 tcaatgatgt aagagtgagt agtacttata ttagaagttt agtatcccag ggtgatatgg
    15601 ctaatgtcaa ggaatatttg ggtagaaatt acaagttaga aggttgtgtt atacattcta
    15661 aacaccttgg aagaactata ggattcccaa cggcaaatat tgaccttaag aataatatgc
    15721 tcgttccaaa gagaggtatt tatgcttcta ttgtacatat tggagatgag acttattttg
    15781 gtgctacaaa tgtaggctat aacccaacag ttaatggaaa aactttgtct attgaaacaa
    15841 atattttaga gtttgataga gatatttatg gggaaaatat aattgttgaa tttttggaaa
    15901 gaataagaga tgaaagaaag tttaattcaa tagatgactt aaaaaatcaa ttgtataatg
    15961 atacaaatta tgtgtatgaa aactatgttt gcaaaaacaa gtaatgtatg ataaaatgat
    16021 taaggaaaat aaccattact cggcatttga atactccaat caatgcttag taataggcgt
    16081 ttctaaaaaa atacggaggt aatctaaatg ataaacaaat cagaaataat aaaagaatat
    16141 ggaagagctg aaggggatac tggttctcca gaagtacaaa tagctttatt aacagctaga
    16201 ataaacgagt taaatggaca tttaaaaact cataagaaag accaccattc aagaagagga
    16261 ctattaaaaa tggttggtag aagaagaaac ttattagctt acttaaagga aaaagatctt
    16321 gaaggatata gagcattaat agctaaatta ggattaagaa agtaatttca ttctttataa
    16381 aaaagcaggt atagtgtata cctgcttttt tatagttaat tcattataaa gtaaaatagt
    16441 taataataaa agtagtttat atgttataaa aaattataaa atgaataata ttgggtaaga
    16501 atattaaaat agtatacaaa tacataatca atttaaaagg aaagacagga taaatataga
    16561 aattttataa caggataatt agttggaata aggaggatat tattttgaaa ataaagttag
    16621 tatgcgatag tttgtgcgat attccagatg aaatatcgga aaaagattat atagagatag
    16681 ttccacttac agttattttt aatgataggg aatatataga aggagtagat ataaagaaag
    16741 aagagtttta taaaaaagta aaagaaatca agcaaatacc taaaacttca caagctacat
    16801 atatggagtt taaagaagtg tttgataagt tcataacaga aggttatcat ataatctgta
    16861 tgacaggggc tgcaaacgca tcaggaactt ttcaaagtgc tatgatagct aaaaatgatg
    16921 taaatgaaaa tgaaaaaata catatatttg atactagaaa tttatctcta ggtagtggac
    16981 aatatgttat aaaggcatgt gaacttcttg aagaaggact gggatttgaa gaaattatag
    17041 atgaattaga aaatactcgc agtagtgtaa gattgctttt tgcaccatat acacttgatt
    17101 ttttaaagca gagtggaaga gttccagttg caactgcttt gataggaaat atgttaaata
    17161 taaagcctat atttttcttt gataatgggg aagctaagtt ggtaaataag gttagaggaa
    17221 taaagaatat agcaagtaaa ttggtcgata taattttaga aatgaatgaa gggtctttag
    17281 aaggaaaaat cgtcacaata ggttgtggag ataatttaca cgattgtgaa atattaaaag
    17341 atgaagtaaa taaaagaata aaagcaagaa gagtattatt tactcgagga ggagtttcta
    17401 tttgctctca tacaggacca gatatattgg ctataagttg ttctaattag tagtaatata
    17461 aggtttttgc atatatatgt gaagataatt ttatattgtt aatttatagt atttttgata
    17521 atatagtaaa tagagtgata tacgtgtttt ttaataagag gaggacaagc attaatgttt
    17581 gaacataaaa tatttaaaat ggattttgca ggaagagaac tttctgtaga aataggaaag
    17641 atttgtgaaa tggctagtgg tagttgtata gttcgataca gtgatagtat ggtaatggta
    17701 aatacaacta aatcagccaa acctagagac ggaatagact tttttccttt aagtgtggat
    17761 tatgaagaaa aattatactc tgtaggtaag atacctggag gttttttaaa gagagaaggc
    17821 aagccttcag agaaagctat acttacatca agattgatag atagaccaat aagaccttta
    17881 ttcccaaaag gatttagaaa tgatgttcag gtagttgcaa cagttctatc agtagaccaa
    17941 gattgtacac cagatatagt agcaatgata ggttcttcaa tagccctttc tatatcagac
    18001 ataccattta atggaccaac aggttcagta tgtgttggcc ttgtagatgg tgcatttgtt
    18061 gtaaatccaa acgcagagca aagagaaaaa agttcaatgc atctagttgt ttcaggtaca
    18121 aaggaagcaa taatgatggt tgaagccgga gctgatgaag ttccagatga ggtaatgctt
    18181 gatgctattt tatttgctca ccaagaaata aaaaaaatag ttgagtttat agaaggtata
    18241 gttgcagaag ttggaaaaga aaaaatgcct gttgaattat accatgcagg agaagaaata
    18301 actcaattgg ttcgcgaatt tgcaactgat aagatgaaaa aagcagttca gacttttgaa
    18361 aagttagaga gaatggaaaa tatggataga gttaaagaag aaactttagc tcactttgaa
    18421 gaaactttag aagattttga agattttgtt ggagatatag aagaagtatt acaagatata
    18481 ataaaagaag aagtaagaaa acttatagta catgaaaatg taagaccaga taatagaaaa
    18541 cttgaagaaa taagacctat atggtgtgaa actggcatga tacctagagc tcatggttca
    18601 gccatattta ctagaggaca aactcaagta cttaatgttg ctactttagg tgctttagga
    18661 gatgttcaaa aactagatgg gttagatgaa gaagaaaaca agagatatat gcatcattat
    18721 aatttccctg catatagtgt tggagaagct agaccatcaa gaggtccagg aagaagagaa
    18781 ataggacatg gtgctttagc agaaagagct ttattaccag taataccatc tcaagaagaa
    18841 tttccttatg ctattagatt agtttctgaa gtattaagtt caaatggttc tacatctcaa
    18901 gcaagtgtat gtggttcaac attatcttta cttgatgcag gtgtacctat aaaagatatg
    18961 gttgcaggta tagcaatggg acttataaag catgatggta aggttgcagt gctttcagat
    19021 atacaaggta tggaagacca tttaggcgat atggacttta aagtagctgg aacagagtat
    19081 ggtataacag caatacaaat ggatataaaa attgatggta tagataaaga aattctacaa
    19141 cgtgctttaa aacaagctaa agagggtaga atccatatac taggagagat gagaaaaact
    19201 atatctcagc caaaaccaga actttcacca tatgcaccta agattgttaa aatgcaaata
    19261 aatcctgaca agataaaaga tgtaatagga cctggtggaa agataataac taagataata
    19321 gacgaaactg gagttaagat agatattgaa caaactgggg aagtatttat atcaggtatt
    19381 gagattgata tgattaagaa agctcaagaa cttataaata atatagtagt tgaaccagaa
    19441 gtaggaaaaa cttataaagg taaggtaagt agaataatga actttggagc atttgttgag
    19501 atacttccag gaaaagaagg attacttcat atatctcata tagctcatga aagagttgcc
    19561 aaagttgaag atgtattgaa tataggggat gaagttgaag ttaaagttac agagattgat
    19621 gaaaaaggaa gagttaattt atcaagaaaa gttcttttac caaaaccaga acataaaaat
    19681 aaataactta ttatataatt aaaagcaaag tcttaggatt ttgctttttt aaatacaaaa
    19741 aagtagataa ttttaaaatg atagttatgg atggcttata gttctttttc tgtcagtctt
    19801 tactcttata aagaagctat ttaaaagctg attaatagaa agctttattg atatatatag
    19861 aatcacttaa gttatattta gtgcataaac agagcttatt tttaatagta ttaaataaga
    19921 aacaatttgt acataattag ggggagtttt aatgataatg atggtactta ataagagtaa
    19981 gctcaaaaaa atcataatac taattttagt agtgttatta gtattatcag gaggggtaat
    20041 ggtattaaag ttatttaaca acaagccagt atttaattta tttaatggtg tagatttacc
    20101 atatgaaaga ggtacaaagg ataaaagtgg atatgttgca ttaacctgta atatagacct
    20161 tggatgggaa acagagtacg tagaatccat tttagaaaca cttaaaaaag aaaatgtaaa
    20221 gattacattt aatgtaactg gaaaatgggc agaaaagaat aaggatgaat tattaaagat
    20281 aaaaaaacaa ggacatgaaa taggtaatca tggatataaa catttagatt attctacatt
    20341 atcatatgaa gacaattatg agcaaataga gacctctaaa aagataattg aagaaattat
    20401 aggtgaaaaa actaaatttt ttcaagcacc agcaggttcc tttggtccag aaacagtaaa
    20461 ggctgctaaa gcattagggt atacttctat caaatgggat gccgatacta tagattggaa
    20521 atacaaagac caaccagagg taatcattga tagaatgaag aaaaaagaca ttaaagatag
    20581 tagtataatc ttaatgcatc caaccaacgc aacgacaaaa tgtatagatg atattatagc
    20641 tattgtaaga gaaaaaggac taaaagcagg aaaacttagt gatgtattta agtagacaaa
    20701 gtataaatat ttggttaaga ttgtataagt tgtagttgaa ttaattaaaa gactgactta
    20761 taatgtaata catataaaaa ataaatataa ttaaaacata gtgttttcaa taatccaata
    20821 tgaaacaaag aataaattgt taggagaatg taatgtataa gacaaaaata ctggaaaatg
    20881 ggcttacaat aattggtgaa gaaataccat atctaaagtc aataacatta ggtatttgga
    20941 taaatgcagg ctctaggata gaagaagcac aggtgagtgg aacttctcat tttatagagc
    21001 atatgatgtt taaaggaacc aaaaacagaa catcaaaaga gattgctagt tcaatagata
    21061 atttgggtgg tcaaataaat gcatttacaa gtaaagaatg tacatgttat tatgtaaaac
    21121 taatagatga gcatatagat acaggaattg atgtattgag tgatatgata ctaaattcta
    21181 aatttgataa aaatgatata gataaggaaa gacttataat attggaagaa cttaagatgt
    21241 atgaagactc tccagatgac ttatcttatg atttattagt ggaaaatata tatgctaatg
    21301 atggattagg aatgaatata attgggacaa aagaatctct atacaatata actagagaat
    21361 ccatgttaga atacttaaat aagtattata ttccaaataa cgctgttatt tctatagctg
    21421 gaaactttaa ctttgatgat atggtggaga aaatcaaatc aaaatttgga cactgggaaa
    21481 agaaaaattt gagtatagac ataagtgagg ctaagtttaa tccttgcttt atatcaaaaa
    21541 ataaagatac tgaacaggtg aacttagcta tgtgcctaaa aggtattcct tttgaaaatg
    21601 atgaagaagt ctattcaatg gcagtggtaa ataatatttt cggtggaagt ataagctcaa
    21661 ggctttttca gaaaataagg gaagaaaaag gtttagtata ttcaatatac tcatcacaga
    21721 cattatatag gaaatgtggt gaattaggta tatttgcaag catgagtaca gaaaatttac
    21781 aagatgtata taatttaata aagaaagaaa ttgaaaatat aagagaaaac tatcttacag
    21841 aaaaagaaat atctgaaagc aaggaacagt taaaaggaaa ttatatattg gatttagaaa
    21901 gtactagcag tagaatgatg tctactggta aatctatgtt acttagtaaa aaagtaaaaa
    21961 ctacagatga aatacttgaa tgtataaata gtgttaatat taattcaata aagaaggttg
    22021 ttgataaggt attcaatatc gaaaatattg gtacatgtat agttggaaga gatgtagaaa
    22081 agattctaca cttggattaa tattgaaaaa agtaaatctc tggaggtata aaatatgaat
    22141 ttatctgaaa tagcaggtaa ggaaatagtt aacttggtca caggagaaag attgggggtc
    22201 attggagaat gtgaccttat actagatgaa actacaggcg gtatattagc gcttctaatt
    22261 ccaaaagaaa aaggattttt tgccagaaga aaagataagt catttttaga agtaccatgg
    22321 agaaatgtaa aaaaaatagg taatgacatg ataattatag aatatgaggg atatatttag
    22381 gaggaaaaaa tgagtatttt agtgcaaaag tttggtggga catcagtaga atcatatgaa
    22441 aaaatgaatg aagtatgtaa gatagtaaaa gcatacaaaa aaaatgatga agaattacaa
    22501 ttagtattag tagtttcagc aatgggaaga aaaggagctc catatgctac agatacactt
    22561 ataaatttgt gtagtgcagt aaatgatgag ccaagtaaaa gagaacttga tttaataatg
    22621 tcttgtggag aaataatatc aggaacaatt cttgctaatt tattgaatgc acaggggata
    22681 gattctgttt tctttactgg tcagcaagca ggtataatta caagtgatga atattctaat
    22741 gcaaagataa agtatataaa tcctaaaaag gtaaaaagag ctttagatga tggtaaggtt
    22801 gtagttatag ctgggttcca gggtgtaaca gatgatggag aaataactac tcttggaaga
    22861 ggcggaagcg atacatcagc agttgctatt ggtaaagctt tagaatgtga aacagttgaa
    22921 atttacactg atgtggatgg tataatgact gcagacccta gagtagaacc aaacgctaaa
    22981 gtattgagtt ttatagacta tgaggaagta ttccaaatgg ctgacaaagg tgcaaaagta
    23041 atacatccaa gagcagtaca gttggctaaa agtggaaata taactttagc cataaagaat
    23101 acaatgaatc caacttttga aggtacaaaa ataggttctt tatgtagaca cttagaagac
    23161 aatatagaat atgaacaaga aagagatttt aaggttgctg ttgcaaataa agatagcgta
    23221 gcacaagtta agattaagtc agcagaagaa gtgtttacag aagtattaaa tgagatagaa
    23281 aagaaactta taactataga tatgataaat ttctttatat cagaaaaagc atttgttgta
    23341 gaagatgctg atataaaatc attaaaggaa atactagata aatttgaatt agattatgaa
    23401 gtaaatagag actgtgctaa ggtaacattg atatgttcaa gaattgctga tgaaatgtct
    23461 ggtatcatgt caaaggtagt tagaggatta tcaaaagcag gggtatccct tttacaaaca
    23521 tcagactcaa atatgactat atcttgttta gttagtgaag aagatatgca tacagcagta
    23581 catgctatac atcaacaatt ttatttaaaa taaactgtat aatacaatta ttttttaatc
    23641 aaatttaaga atttcatata tatcttaata tatttgattt atttggtaaa actaaattaa
    23701 atataatttt gataggagtt tttttatgaa agattataga gaagataatg aaagatttga
    23761 taaagataat tgtagtgaag aatctagttg cgaagagaga gaatgtataa agcaatttgg
    23821 taccaatgaa atgccacctc aacctcctaa agatattcaa tgtataacta taattggaga
    23881 aattgaggga cattttatag gaaatcctca aaaaaaagct acaaagtacg agcatataat
    23941 tccaatgcta tactcaattg aggaaagtaa tgatgttaaa ggagtgttag ttgttttaaa
    24001 cactgttgga ggagatattg aagcaggttt agctattgca gaacttttaa acagtacatc
    24061 taaaaaagta gttactcttg ttctaggagg aagtcatagt ataggtgttc ctcttgctac
    24121 agcaggagat tactcattta tagcaccaac agcaaccatg attatacatc cagtgcgaac
    24181 tactggtctt gtgattggga taaatgagac ttttgagtat tttaaaaaga tgcaagatag
    24241 aataattcaa tttataataa gaacatcaaa tatcaaaaaa gatgtacttg aaaaacttat
    24301 gcatgaaaaa gatgaacttg taagtgatgt tggtagtgta ttaataggaa aagaagctgt
    24361 ggattatggt cttatagatg aagttggagg actaaaagaa gcacttaaaa aattaagaga
    24421 gcttataaaa gaatctgagg aagaaaaaaa taatgattaa aatttgttta aataataaaa
    24481 tttatgatat aatgatgtaa taaagtagga gtgttagtgt taaggcatta gcactttttg
    24541 ctgtgaaggg gtaaaacttg ctaaaaataa ggagtgatat taatggccac gaaaaagaag
    24601 aaaaaaaaga aggtaaatac cttaagtttt aatgcagaat atcataattt aataacaata
    24661 tttataggtg tatttttatt atatagttta aattctaatt ctatgggatg gatacctgtg
    24721 ttaatgcaaa atctttttaa gggactattt ggagggcttt caatagcaat accatttata
    24781 gtaattataa caggactttt aggatttttt gatggaaatg aatatatata taggctaaga
    24841 aagactaaac tatactacat aattatttta tttatatttg tattttatgg attattaaat
    24901 gcaggaactc ttcctgtaga tagcccacta aaaggtaaca tgtttgatga tgttatgaaa
    24961 ttgggtgtaa gtggacaagg tagtggtttg atagcaacaa ctattgcgta ttatatgagt
    25021 aaaatatttg gtatagcagg tggatggtta ataagtatct tcgcacttat actttctgta
    25081 atgtttatat ttaatatttc tataaaagat ttggtgtcaa atgcaaaatc aaaatctaaa
    25141 gcatctaaag acagcaattt aacgtttaaa gataaaattg caaatatgaa aaaatctgca
    25201 atagatatga tgactgatga agtagatgat actactataa ataaaaaacc aggtttcttt
    25261 aaagggttaa tgtcaaaagg tcttaataat gatgatgaag aagatgaata ttttgaagaa
    25321 gaaaatagtg atggtgtaga tgataaaact ataaaaatag ttggatttaa taaggcagaa
    25381 gatgagtatt tagaaatatt agaaggaact caaagtatgc cagagttaga tgttttaaaa
    25441 gaattacaaa aagctaccaa tgaaaatcca gttgtagata ctaaaccaga aaaaaaagtt
    25501 gatatagcta aacctaattt aaatatagaa aaaactcaac ctatgagtat tgtagcagag
    25561 ccagttaatg aggattattc aaactataaa aagcctagca ttgagctttt aaataaagta
    25621 aataagaagt ctgatgaaaa tggtaaaaag aaagtgttga aaaatgcttc tcttttagaa
    25681 aagacactat cagattttgg agtggaagca aaaattaatc aagttacagt tggacctact
    25741 ataacaagat atgaaataca accaagtcca ggagtcaagg taagtaaaat tgtaaattta
    25801 actgatgata tagctttaag tttagcagct aaaagtataa gaattgaagc cccaattcct
    25861 ggtaaaagtg caattggaat agaagttcca aatgaagaag ctcaaatggt tggtgtgaga
    25921 gaagttctag aaagtgaaga atttaataat tttaactctc ctcttgcaat gggacttgga
    25981 aaagacgtag caggaaaaat tataattgga gatataggta agatgccaca cttactaata
    26041 gctggttcta caggttcagg aaagagtgtg tgtgtaaata cattaataag tagtatttta
    26101 tataaggcaa atcctgatga agtaaaatta ctattaatag acccaaaagt agttgaactt
    26161 gcaaattata atggaatacc acatctattg ataccagtag ttactgaccc taaaaaagct
    26221 gcaaatgctt taaattgggc agttacagag atgaatagaa ggtacaaatt atttgcagat
    26281 gctcaagtaa aagatgtaac aagttataat gagaaagcgg aagaaaaact tcctaaaata
    26341 gtaataataa tagatgagtt agcagattta atgatggcaa gtgcaaatga tgtagaagat
    26401 tatatttgta gattagctca aatggcaaga gctgctggaa tgcacttgat tgtagcaact
    26461 caaagaccat ctgttgacgt tataacaggg gtaataaagg ctaatatacc atctagaata
    26521 gcatttgcag tatcatctca aactgattca agaacaatcc ttgatatggg tggtgcagag
    26581 aagcttctag gtaaaggtga tatgttattc tatccacttg gtgcagcaaa accagttaga
    26641 cttcaagggg cattcatatc agagagtgag tcggaaaaag taatcgattt tgtaaaaagt
    26701 caagttaaag atggaataaa atatgaagaa gatataattg aaactatatc taaggttaat
    26761 acttcaaagg gtagtgatga ggatgagttt ttgagtgaag cgatagaatt tgtcgtggaa
    26821 agtggtcaag catctgcctc tatgctacag agaagattta aaataggatt caatagagca
    26881 gctagactta tagattctat ggaagaaaga ggaataattg gagcaagtga gggaagtaaa
    26941 ccgagaaagg tattaataag caaacaggat ttgcagaatt tagaaggtga atagaattgt
    27001 cagttataaa aatagaaaaa gcacttaagc tagataaagt aatatttgta gatgtgagaa
    27061 ctgaaggaga atatgaagaa gaccatatat tgaatgcaat caatatgcct ctttttaaaa
    27121 ataatgaaca taatgacgtt ggaacaattt ataagatgca gggaaaacat gaagcaatac
    27181 aaaaagggtt tgactatgta tcgtataaat taaaggatat atatttacaa gcagcagaac
    27241 ttgcattaaa ttatgataat attgtcatat attgtgcaag aggcggaatg agaagtggca
    27301 gtatagtaaa tttattatca tcattaggtg taaatgtata tcaacttgaa ggtggatata
    27361 aggcttatag aaattttgta ttagagtatc taaggcatgt aatggataca aaaaactttg
    27421 tatctgttca tggattgact ggtacaggaa agactgattt gttacattta ttagatgaaa
    27481 aaggaataga tgtactggat ttagaaggca tagctaaaaa cagtggttct acctttggtt
    27541 ttataacttt tgataaaaag ccaccttctc aaaagaactt tgaaacaaaa ctatttgaaa
    27601 gtatattttt ttcgaaagag aattatatat ttgtcgagag tgaaagcaaa cgagttggaa
    27661 gtgttacagt tccaaatgaa attttcgagg caatgacacg agaaggacat catatattat
    27721 tagaatgtga tattgaaaat agagttgata gactttgtag agattatatt tatgataaag
    27781 atgaaggtaa tatattgatt ttaaaagaat gtataaacaa gtttagaaaa agattgggta
    27841 ataaagtagt agatgattat attgatttat tagaatcagg tgaatataaa gaacttgtaa
    27901 aaagatatct tttagaatac tacgacccac tttatatgca ttcagtagaa aaatataagt
    27961 atagtaagat tataaatttt aatgatacac aaaaagcttt agaagatgtt atagacttat
    28021 atgagagtat attggaagga gaaagtgaat gttaaaaata gcattagaat cattaggatg
    28081 ctcaaaaaat ttagtagatg cagaaataat gatgggaatt ttaaataaca aaggctataa
    28141 attaattgga gattttgaag aagctgatgt aattatagta aatacatgtg gatttataga
    28201 gtcagctaaa caagaatcta tagatacgat aataaacttt gcagagttaa aaaaaactgg
    28261 caatttaaag ttgttgatag taacagggtg tctagcccaa agatattctg aagaattaaa
    28321 aactgaaatt cctgaaatag atgctatagt aggaacagga agctatcaaa atatagataa
    28381 aatacttaaa gaattaagtg aaatgcataa aattgtaagc cttaatgata tagagtttgt
    28441 atttaatgaa gatttaccaa gatatatatc tacacctagc tatatggcct atttaaaaat
    28501 tggagaagga tgttccaata attgtactta ttgtataata ccaaaactaa gaggtaaata
    28561 tagaagtaga aaatttgaag atataattaa agaagctaag aaattagctg aatctggagt
    28621 aaaagaactt gtggttatag cacaggatac gacaaagtac ggttttgatt tatatggaaa
    28681 agaaagatta tcagaacttt tagaagaact tgcaaaaata gatggattta agtggataag
    28741 ggttatgtat tcatatccag aatctataac agaagaattg atacaagtta taaagaaata
    28801 tgataatata tgtagttact ttgatatgcc aatacagcat gcaagtaaca atattttaaa
    28861 attaatgaat agaaaaacta ctaaagaaga tatattaaat aaaatcaatc tgataagaag
    28921 taatatacca gatgctatac ttagaactac aataatagta gggtttcctg gtgaaacaga
    28981 agatgacttc aaacaacttg tggactttgt tgaagaggtt aaatttgata gacttggtgc
    29041 atttgcatat tcaagagaag aagatacacc agcagataga ttgccaaacc atatagatga
    29101 agaagtaaaa atacaaagaa gagatacttt aatgatgatt caacaaaaaa tatctgaaga
    29161 acttaatgac aaaaagatag gaaaaactta tgaggtgtta atagaagaac agatagaaga
    29221 taatgtatat acaggaagaa cacaaggtga tgcagaggaa attgacagta tagtgtatgt
    29281 aaaaagtgta gacaatcttg aagtaggaga atttgtaagt gttcaaataa atgatgcaat
    29341 ggaatacgat ttaatgggag atgttttata tgaacttgcc taacaagtta actttgttta
    29401 gaatattttt aataccagta tttgttttaa ttatgttgct caatatacca aataaatttt
    29461 taatagcttg tattattttt ataatagctt caattactga tgctttagat ggaaaaatag
    29521 ctagaaaata caatctagtc actgactttg gaaaatttat ggacccatta gcagataagt
    29581 tattggttat ttcagcactt acatgtatga tagaagacca tcttgtaagt agttggatgg
    29641 taattataat agtggctaga gagcttacag ttagcatatt aagagctata gctgctgctg
    29701 atggaaaagt aattgctgct ggaaacagtg gaaaacttaa aacgataact caaatggtat
    29761 ctatagtgtt tttattgtta ggtgctcagt ttgaaaatgt attgatatta aatattggtg
    29821 aaattctcat tcttatagct acactactta cattatattc tggatgggaa tatctatata
    29881 aaaataaaga gctttttatg tcttctaagt aaaaaattat gctttaataa ataaaaacta
    29941 atgattaata aataaaaaat aacaacttaa aattttaaaa atgtaaaaaa gggtatacta
    30001 ttatagtata cctcttttca ttttaagaat attttctttt gtgtatataa ttattaataa
    30061 tggtttataa ataaaatgta atgaataaaa atataatcat tagtagcgtt tttaaattga
    30121 ctaggtttga tttatgttat ataattaata tataacaaga gaacaaatgt ttgtagataa
    30181 atgaagggat ggtatagaga tgagtgtaga tcaagaaaaa ttaaaagcgt tgaatgaagc
    30241 tttaggtaaa attgaaaaag attttggtaa aggttcagta atgaaattgg gagaagcaac
    30301 atctatgtct atagatgtta tatcaacagg agcgattggt ttagacatag ctattggtat
    30361 aggaggtcta cctagaggga gaatagttga agtatatggt ccagaatctt ctggtaagac
    30421 tactgttgcg cttagttgtg tagcatcagc tcaaaaagat ggaggaatag ctgcatttat
    30481 agatgcagaa catgcacttg acccagtata tgcaaaagct ttgggtgtgg atgttgataa
    30541 cctaataata tctcaaccag atacaggtga acaggcttta gagatagcag aggcattgat
    30601 aagaagtgga gcgatagata taatagtaat agactcagta gcagcattag ttccaaaggc
    30661 tgaaatagat ggagatatgg gtgattctca tgtaggttta caagctagac ttatgtcaca
    30721 agcacttaga aagttaactg gttcaattaa aaaatcaaat tgtgttgcta tatttataaa
    30781 ccagttaaga gagaaagtag gaataatgtt tggaaaccca gaaactacta ctggaggacg
    30841 tgcactaaaa ttctattcat cagttagatt ggatgttaga aaaatagata caataaaaca
    30901 aggtgataaa gttataggta gtagaactag agttaaagtt gttaaaaaca aagtagcacc
    30961 accatttaag caagctgaat ttgatataat gtatggagaa gggatttcaa aaattggaga
    31021 ccttttagat atagctgctg atgtagatat agttaaaaaa tcaggttcat ggtatagtta
    31081 caatgatact aaacttggac aaggaagaga aaatgttaaa aaattcttgg aagataattt
    31141 agatttaact actgaaatag atgagaaagt tagagcattt tacaatttaa atgaagaaca
    31201 tgaagaatca ggtacttcag tatcaaaaga aattgtagaa gaataagtaa aaatacaaaa
    31261 aaataactag taattaaaga catgccacgc tccctattag cttgacacaa tgttgaaaaa
    31321 attataaaat taagtgagga taaaatatct aaatttaatt ttaattatta ggaatatttt
    31381 ttaatcatag tgatgacatt ttgggattat taacgaagga ggtggatgtc atagatagtg
    31441 tagtaatgat tgtaataggt gcagctgttg gtattatagc tggatatttt gttagaaaaa
    31501 atatatctga agcaaaaatt ggacaagctg aaaacttggc caaagaaata atagataaag
    31561 cacaccacga ctcagaaaca gtacaaaaag aaaaattact agaagctaaa gaagagatcc
    31621 ataagtggag aacagaagct gaaagagaaa acagagaaag aagaacagaa gtacaaaagt
    31681 atgaaaaaag agtagtacaa aaagaagaag tcttagatag aaaattacaa aatttagaat
    31741 ctaaagaagt aaacctaagt gaaaaattaa aggtagtgga aaagaaagaa gaagaagttg
    31801 aagtaataaa aactcaacag ttagaaaaat tagaaagtat atcaggaatt acttctgata
    31861 aggccaaaga aatcatctta accaatgcgg agagagatgt aagacgtgaa atgtctataa
    31921 tgataaaaga gatagaaagt caagctaaag aagaagctga taagaaatca agagaaatta
    31981 taggatatgc aattcaaaaa tgtgcagcag atcacgtagc cgaaactact gtaacagtag
    32041 taagcttgcc taatgatgaa atgaaaggta gaataatagg tagggaaggt agaaatataa
    32101 ggactttaga aaccttaaca ggtatagacc ttataataga tgacactcca gaagccgtaa
    32161 ttttatcagg atttgatcca ataagaagag aaattgcaag aattgctcta gaaaaattaa
    32221 tagcagatgg aagaatacat ccagctagaa ttgaagaaat ggttgaaaag gcgagaaaag
    32281 aagttgacaa tattataaaa gaatatggtg aacaagctgc atttgaaact agtgtacatg
    32341 ggattcatcc ggaattaata agattattag gtagacttaa ctatagaact agttatggac
    32401 aaaatgtact taaacattct atagaagttg ctcatatagc tggtataatg gctgctgaaa
    32461 taggagcaga tattagattg gctaagaggg caggtttatt acatgatata ggtaaggctg
    32521 tagaccatga aatggaaggt acacatgttg aaataggtat ggatttactt agacgttata
    32581 aagagtctaa agaagttata catgccatga gtactcatca tggagattac gagcctcaaa
    32641 cagtagaggc tgtattagtt acagcagcag atgcaatatc agctgctaga cctggtgcga
    32701 gaagagaaac tttagaagca tatataaaga gacttgaaaa attagaagaa atagctaatt
    32761 catatgaagg tgtagaaaaa tcatttgcta tacaagctgg tagagaaata agaataatgg
    32821 taaaaccaga agctataaat gatgaagaaa tacatctatt ggctagagat atgactaaga
    32881 aaatagagga tgaacttgaa tatcctggac aaataaaagt aagcataata agagaaacaa
    32941 gagctataga gtatgcgaag taaaagaaat aatatctaaa taataagaaa atatttttag
    33001 aaatttaaat ttgccacaag atatgataat atatcttgtg gcttttaaat tgtttaagtt
    33061 taattttata taatgcaaaa ataatctaag tgctaaaagt gctacttaca aaagattaat
    33121 aaaataaagc tttatatatg tattatttat ataattatcc aagtttgttt gatataatga
    33181 agttgtaaaa taaatatatt actacttgga atgcttgtac ttaaataaga tgaaaataca
    33241 atgacgctaa agtgaggatt agagagaggg tacactatga aaaaaaatac acttttacat
    33301 acaaatatta tggtttgcat tattattact cttggttttg tggctacctc tataattgga
    33361 tatcaatcta ataccaatat ttttaaaaat gatattgaac atgtatctac attggctgct
    33421 gaaggtattt attatcagat agataaacta ctttcagaac ctataaatgt atctcttaca
    33481 atggcaaatg acagtctatt aaaaaatttt ttagatggtg agaaagaaca tttaaatgat
    33541 gaagaattta tttataaatt acaagattat ctgaatgtgt atagaaataa atattcatat
    33601 gattctgttt ttttagtatc aactaaaacc aataattatt atcattttaa tggacttgat
    33661 agaaatctta gcgctaataa ctcagagaat caatggtatt atactttctt aaagaatgat
    33721 gatgaatact cattaaatgt tgataatgat gaagcaagta acaatagtat tacagtcttt
    33781 gtaaattgta agataaaaga tgataatgga gcaactatgg gaattatagg tgttgggtta
    33841 aaggttaatt cattacaaat gttgcttaaa ggatataatg acaaatttga tgttgttgca
    33901 cgcttaattg atgataaagg gtttgtacaa ttagcagtag ataaaaccgg tcatgagaat
    33961 ataaattttt ttgaaaattc tagtagcgac ttgtctaatt cgaaacaatt aatattaaac
    34021 aacaaaaagg aacaaaagag tttttggtat tcatctgaaa aatcaaaaag ttatattgta
    34081 tgtcagtaca tacctagttt aaaatggcat cttgttctag aaaatgattt tactttaatg
    34141 attaaacagc ttcatctgca attttttaaa ggcattgcag ttatagtttt aattataata
    34201 tttgtacttt ttactattac acatgtgctt aaaaaataca aaaaccaaat aataaagctt
    34261 agtgtttcac agaaattgga atatcaaaaa ttgttgagca aatctactga aggtctttat
    34321 gaaaatattt ttgaaattga tattactaac aataaagctg gttgtgaaga tgagaatagg
    34381 tattttcatg aatttggatt agggcttaat accccatatg atgaagcctt acatacaata
    34441 gctaaaaacc aaatcaaaga agaatatgca caaggttatt tagatacatt tttacctgaa
    34501 aatgtaatta aacgttataa tagcggaatt aataatatca gctatgattt tttaattaaa
    34561 aaagatgaag aaagttatca ttggattaga attaatgcac gaattttctt ttggacatca
    34621 gatgaatctg ttcgtatgat atcttatcgt aaaaatatag atgaagaaaa aaatagagaa
    34681 ttactacttc tcgaacgttc acaacgtgat tctcttacag ggctttacaa taaagctaca
    34741 acagaggaat ttattcgttc atttttaaaa aatgaaagta atcaagattt agaacacgtt
    34801 tttctaattt tcgatattga taattttaaa aatgtaaatg ataaatatgg tcatgtattt
    34861 ggagactttg ttataagtga atttgcctta gaattaaaat ctcaatttcg tgaggacgat
    34921 attgtgggac gtattggtgg agatgagttt gtagtattaa taaaagactt taataataat
    34981 aactatgtgc ttatagacaa attagagcgt ttttgttcta agattaatca gaaaaaattt
    35041 gttgagcaag cagaattttc tattgcatgt agtattggtg tagcaatgta tcctgaacat
    35101 ggatatgtat actctgaact atatgagaaa gcagaccaag cactttacta tgcaaaaagc
    35161 catgggaaaa attcttatca tatattttca gaggattcta ttgatagtat ttcatttcat
    35221 atagactcta gagatataaa agtattatta gaaatggcta ctgatggtgt tgtcaaattt
    35281 gcttgtatgg aaaacttaaa attactttat ttcaaccaaa aaatgcttga atttactgga
    35341 tattcagctc aaactgttac ttctataggt tttgatattt tttcgcctgt attacaagac
    35401 gacttacaaa tggtactaga tacattaaga gaagcaattt ctaaaagaga gacttttgaa
    35461 atagtttttc gtttttgtca tacagatggt catatttttc ctgtaaggat gcaaggtaag
    35521 tttgtaagtg aattatatca aaatcgttat cctgtttttt atgcgattta tacagacata
    35581 tcaagtatca atactttact agaaaataaa tagtaaatac tacagtaaaa tctaaaaata
    35641 ggatataata gtattaatat aagaagatta taaataaaac ctgctaatga aaatagatta
    35701 tttttagcag gtttttaaat tttttataat aaatcttatt tttggctaac aagatttatt
    35761 ttttttaaga ctttacttat tattttaagc attaattatg aaatttatca ttttattatt
    35821 tacagaaagt attttataac aataaatatc tatacttatt gcaaaatttt tttagcagtt
    35881 ttgtatttat ttgtatttat tttgaattta tttactaaat ttgcctataa aaagtatata
    35941 taaaaatata gttcaaaatg gtataattaa gtataacaga aggaggtaat gtaagtgagc
    36001 caatacataa atataaattc tcagttagac gccctaaaat tgaaggagtt cgagaaaata
    36061 ttttacgaaa atatcaatga ggacgttaaa gtagtgccaa aagttactcc ttttaaagga
    36121 ataaataccg atttgttgta tgttaaagat ggaagaattt tatttataaa atttatggat
    36181 acaacagaag atatattttt tattttagaa gaagagttac ttgaagttat gaatgaggaa
    36241 tatgaacttt tgaaactaaa aatgggacaa aagaatagaa atataagtta taactacgtt
    36301 tacattatgc catacgttga agttgaggaa acatatgaat ttgaagaatt tgttaataat
    36361 aatataatag ataaaaataa gcttcaagat ataatgaata agggttcgct ggaggaatat
    36421 cttaatgatg agaatgatga aataaactta aatttatttt tactagacat atgttcagaa
    36481 tactatataa taaatgataa acttcatcta aatgaaaaat ttaaaaaaat ttcattctac
    36541 aatgatgact ataagtatac agcaactatg atggaagacg ttcaaattaa agatgttgtt
    36601 tctattaaat atgggaatac acttattgaa ggtggttctg gtataggcaa aacagcaatt
    36661 atgctttcta gggctataaa attagcaaaa gtatatccac atcataaatt agtcatattt
    36721 acacatacaa aacaactgtg caatgagctt cgagagagga tagagttatt atacaaagat
    36781 aataacaatt tagaagttca cacatttagt tcttttatat ttaaactagc taaaaaattc
    36841 aatttaattg tagattataa catgttaaaa aatgattacg aaaagacctt taataatctt
    36901 gtaaaacaag cacagaacat tataaaaaat aaaaatatgt tcaaggctat ttttattgat
    36961 gaagcagaaa gctttttaga gtatgaaata gattttataa gggagttttt atataaaaca
    37021 aaattcatat ttaatgtgtg ttcttgtaac tccttgaata tctctaatag attgaatata
    37081 tttaaaaaat tatataatgg tatagaattt gatgataaga ttatcttaag taaaaattat
    37141 agacaagcta aagagattgt agattttaca aataaatttt caaataattc taactcatat
    37201 atcaacgaat taagaccaaa tacagaattt agtactttct tttatactaa agcacttaga
    37261 ggtggaaata agtctgttga tataataaaa gtaagtgacc ttgatgacca aataagttct
    37321 gtaatctggg aaattgagta tttaataagt aaaaaaggtc ttgattattc tgaagttgct
    37381 atcgtttatc cttacaataa gaagaaatta aaaagtggaa aaaccattta ctttcaatat
    37441 atgttaaaaa aagcattgga agaggcaaaa attccttata tatgtgcaga agataatttg
    37501 acaaatataa gtaagaaggt aggaactaca atagcaaata tatatgcaat aaagaattta
    37561 gagtataaag cggtaatagt atgtgaatta gagatgttat acaatcaaac tataaatgat
    37621 attgaacaag attatcaagt aaatgacttt gtaggagatt taaataaggt ttatttagct
    37681 atgagtagag caacagacta cctaagtata gtaacaactt ttaatgaaga agcaagtgat
    37741 ataataagac ttattacaga atcaaaagat atttagtaaa aaaatatttg taatttaaga
    37801 aaataaaaag agactgagat ttgtaaaata aactttacca attcagtctc ttatttttta
    37861 gtctactttc ccaatattat caagaggtaa tagtctcacc ataaccttac caagaacttc
    37921 atcttcatca accataccta catcagggaa tctactatca ttactatttt ctctattatc
    37981 tcccattgca aatagtttac cttttggaac aacagtatct atatctccag aagtatagtt
    38041 attgtgtata tatggttcat ttaataattt tccatttaca tacactttag aatttgatat
    38101 ttttattctg tcaccttcag tagctataac tctttttatt aagtcttttt tctttcctgt
    38161 ttctccatca actaaattgg ttttaaaaac tacaatatcg cctttttcag gtttaccaac
    38221 cttgtatgat atcctattta aaatcagata atctttatca tctaaagtag gatacattga
    38281 ttctccactt acaatagaag gtcttataaa ttgtagaatt acaaatgcaa gtacaatagc
    38341 catagctatt gacttaatcc aatcaaatat ttctttttta acactcataa cttttctcct
    38401 tatcatttgt tattaaatta gaatacatta taatagaaag aaataagcaa gaaccactat
    38461 acccaagact attctataat aaccaaatac tttgaagtca tgttttttga tatagtccat
    38521 aaaaaattta ataactacaa ttgaaacaac aaatgctact acagaaccaa cacctaaaat
    38581 aagccattca aatccagtaa atccaaatcc agcttttaga agttttaatg cactagctcc
    38641 aagcattgta gggatggcta agaagaatga aaactctgct gctacatatc tagaagtacc
    38701 aagtaatact gcacctatta tagtagaacc agacctagat gtacctggta taagagctag
    38761 acactggaat aatcctatac aaagagctaa tttatatgta acttgagaaa aatctttata
    38821 cttaggtttt ttatttctct tttcaagccc tatcattatt ataccatata caataagagc
    38881 aatagcaact actgttgaat taaagaataa tctatctata tcatcttcaa acagtattcc
    38941 aataactcca gatggtataa ctgcaattat aaccttaagc cataaacgga ctgtttcttt
    39001 tttctgctta atattctttg cgctatcaaa aggatttaat tttctaaaaa atataacaag
    39061 tacagctaat attgaaccaa attgaattac aactaaaaat gtacttataa atgtatttga
    39121 aaaattcaat tttataaact catcaactaa tatcatatgc cctgtactac ttactggtag
    39181 ccattctgtt attccctgaa ctatgcccaa aatgactgcc ttaaaaattt caagcaaact
    39241 aaatgtttcc aaatcaaaat acctccaata caaatttata gtagcatatt ttattcatag
    39301 attaataaaa aaatgccacc ttttactaac ttataatacc atagtactgt gtagtgcaca
    39361 atggaagatt gatattttca taaaaccttc ataatataac aaaataaact taaaagttat
    39421 ctattgaata aggggtatat ttgaggggga aatatatggt atagtaaaag tatagctatt
    39481 ttattttatg aaaaggaaag gagttattat gtatagtaca aaaatactag atgattatga
    39541 gtatgaaact tttaatgttt tttctacaaa attaaatcca aacacgaaac acgattattt
    39601 atcaaaggtt atactgttta aagaattttt aaaagggaaa gaattgattt atgctacgaa
    39661 agaagattgt aaaaattttg tagactatat acagactaaa tatgcgaaat caacttgtga
    39721 aaagatatat agttatttac atagttttta taacttctta aaaaaagaag gatacataga
    39781 tataaatcca tttagatatg tagaaaaacc cacagtaaca agaataaaaa caaaagatga
    39841 tgttctaagt atacaagaaa taaataaact tataggtatc ctaccaaaat taaatataag
    39901 agatagagta ataatagtct gtctagttac tactgggtgt ttattaaatg aattagttag
    39961 cttaaagtgg aaagacctta tggtagatga aaacgataac tcatatgtaa ggttaggtaa
    40021 gggaagaaaa gaaagagtag ttaaattaca tccatacttt tttaaacttt tagaagatta
    40081 caggaattat tctgggcttc cagaggtaat aatacctagt gatgatttta tttttacaac
    40141 tcaaaagagt aattctataa cagatagaaa tgtcagactt atagtcaaaa aagcattaga
    40201 tttagctgga ctttctcaat attcagcaag agattttaga cattcatttg cagcaataag
    40261 tttaaggctt ggggcagatg aaagtgatgt aaaaaatcag cttggatgga gtgataaata
    40321 ctatgcaatt agatataagt atgtattaaa ttttgttgat agtgaaatag tggattactt
    40381 aattgaaaaa gataatttgg at
//
LOCUS       DAELTA010000013        38308 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.013, whole genome shotgun sequence.
ACCESSION   DAELTA010000013 DAELTA010000000
VERSION     DAELTA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.013"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            141..1499
                     /locus_tag="KPG13_000623"
     CDS             141..1499
                     /locus_tag="KPG13_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0984367.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            1772..2152
                     /locus_tag="KPG13_000624"
     CDS             1772..2152
                     /locus_tag="KPG13_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0984368.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            2277..2477
                     /locus_tag="KPG13_000625"
     CDS             2277..2477
                     /locus_tag="KPG13_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984369.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            2673..3401
                     /locus_tag="KPG13_000626"
     CDS             2673..3401
                     /locus_tag="KPG13_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0984370.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            3423..4340
                     /locus_tag="KPG13_000627"
     CDS             3423..4340
                     /locus_tag="KPG13_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG0984371.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            4482..5666
                     /locus_tag="KPG13_000628"
     CDS             4482..5666
                     /locus_tag="KPG13_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0984372.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            5773..5961
                     /locus_tag="KPG13_000629"
     CDS             5773..5961
                     /locus_tag="KPG13_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG0984373.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            6110..6760
                     /locus_tag="KPG13_000630"
     CDS             6110..6760
                     /locus_tag="KPG13_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0984374.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            6863..7801
                     /locus_tag="KPG13_000631"
     CDS             6863..7801
                     /locus_tag="KPG13_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0984375.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            7827..8678
                     /locus_tag="KPG13_000632"
     CDS             7827..8678
                     /locus_tag="KPG13_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0984376.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(8963..9697)
                     /locus_tag="KPG13_000633"
     CDS             complement(8963..9697)
                     /locus_tag="KPG13_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0984377.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            9822..10538
                     /locus_tag="KPG13_000634"
     CDS             9822..10538
                     /locus_tag="KPG13_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0984378.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            10672..11364
                     /locus_tag="KPG13_000635"
     CDS             10672..11364
                     /locus_tag="KPG13_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984379.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            11354..12535
                     /locus_tag="KPG13_000636"
     CDS             11354..12535
                     /locus_tag="KPG13_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0984380.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            12817..13272
                     /locus_tag="KPG13_000637"
     CDS             12817..13272
                     /locus_tag="KPG13_000637"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG0984381.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            13262..13987
                     /locus_tag="KPG13_000638"
     CDS             13262..13987
                     /locus_tag="KPG13_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG0984382.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            13959..16340
                     /locus_tag="KPG13_000639"
     CDS             13959..16340
                     /locus_tag="KPG13_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG0984383.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDGKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(16545..17774)
                     /locus_tag="KPG13_000640"
     CDS             complement(16545..17774)
                     /locus_tag="KPG13_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0984384.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            18375..19289
                     /locus_tag="KPG13_000641"
     CDS             18375..19289
                     /locus_tag="KPG13_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984385.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            19313..19948
                     /locus_tag="KPG13_000642"
     CDS             19313..19948
                     /locus_tag="KPG13_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG0984386.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            20324..21040
                     /gene="pyrF"
                     /locus_tag="KPG13_000643"
     CDS             20324..21040
                     /gene="pyrF"
                     /locus_tag="KPG13_000643"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG0984387.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            21066..22109
                     /gene="carA"
                     /locus_tag="KPG13_000644"
     CDS             21066..22109
                     /gene="carA"
                     /locus_tag="KPG13_000644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0984388.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            22162..25368
                     /gene="carB"
                     /locus_tag="KPG13_000645"
     CDS             22162..25368
                     /gene="carB"
                     /locus_tag="KPG13_000645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0984389.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            25625..26674
                     /gene="carA"
                     /locus_tag="KPG13_000646"
     CDS             25625..26674
                     /gene="carA"
                     /locus_tag="KPG13_000646"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG0984390.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            26867..30073
                     /gene="carB"
                     /locus_tag="KPG13_000647"
     CDS             26867..30073
                     /gene="carB"
                     /locus_tag="KPG13_000647"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG0984391.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            30573..31259
                     /locus_tag="KPG13_000648"
     CDS             30573..31259
                     /locus_tag="KPG13_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984392.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            31246..32235
                     /locus_tag="KPG13_000649"
     CDS             31246..32235
                     /locus_tag="KPG13_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0984393.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            32318..33004
                     /locus_tag="KPG13_000650"
     CDS             32318..33004
                     /locus_tag="KPG13_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984394.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            33004..35550
                     /locus_tag="KPG13_000651"
     CDS             33004..35550
                     /locus_tag="KPG13_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0984395.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(35706..36563)
                     /locus_tag="KPG13_000652"
     CDS             complement(35706..36563)
                     /locus_tag="KPG13_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0984396.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(36584..37249)
                     /locus_tag="KPG13_000653"
     CDS             complement(36584..37249)
                     /locus_tag="KPG13_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0984397.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            complement(37249..38121)
                     /locus_tag="KPG13_000654"
     CDS             complement(37249..38121)
                     /locus_tag="KPG13_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0984398.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
BASE COUNT        14807 a         4403 c         6939 g        12159 t
ORIGIN      
        1 atattaaaca agatttatat tatttgaaag cagtatatta taatgcgaac aatacaattc
       61 taaactttgc attgtcaatt tacagttcga taaactaatt aaaaattaaa tttatataaa
      121 gagggaaaag aggaataggg atgtttataa ggaaaaatgt tataaagtta gcaattccta
      181 taatgataga gcagacgttt gtcatgcttt taggagtatt taatacaatg atggcaggtc
      241 atattgggga agaagctgta tctgcagttg gaatggttga ttcaataaac aatatattta
      301 tatctgtatt tgctgcacta tctgttggag ctactgttgt tgtagctcaa caaataggaa
      361 aaaaagatag tgaaaaggtg aatgaaacta ttaagcaggc tctagtgtct ggagtaatat
      421 tatcctttac aataacaata ttgatgtggg tttttagaac tggtgtaatt aatttatttt
      481 atggttccgc agaagaattg gtaaaatcaa atgcaaaatt atacatagag tttacattat
      541 ttacgtatcc atttattgcg acacaacaaa tagcaaatgg tattttaaga gggtgtggag
      601 atgtaaagaa acctatgtat gtgactatgt ttatgaatat agtaaatgta acattgggat
      661 atatacttat atatggtatt gatttaaatc ctctaggaat aaatttgcaa acaccatcct
      721 atggcataac aggtgcagct atatctattg ctatagctag aataattgga tgtatagtaa
      781 ttataagagc tttatttaaa ggaaacgaat ttatatctat gggtaaaata tttccattta
      841 agattgatgt tgaaactcaa aagtctatat ttaatatagg aataccagct ggagttgaac
      901 aactactttt taatgcaggt aaattgattg ttcaagtatt tatagttact atgggaacat
      961 cagcaattgc atccaatgca ataggaatgt caattgcatc cataataaat gtaattggga
     1021 atgctttagc tttgtctgct acaaccttgg tgggacagta tataggacgt ggagatatta
     1081 aaggagctaa gagtacttta ttatatttaa ctaaatttgc aacaatttgt ttatttatag
     1141 taggagctat atttatacca attgcaccct ggatttcaag tatgtatact caaaatgcta
     1201 gtgtaattga catctcaaca caattaattc gaagtgattg tttagctatg gtaatatggc
     1261 ctatatcctt tgtattatca gcagggctaa agggagccgg tgatactaga tatacaatga
     1321 tgactgcagt aataggtatg tggattttta gaatttttac aggttacgta ttgggggttg
     1381 tattagaatt tggagtgtta ggaatttgga taggtatgta tactgattgg cttgttagag
     1441 ggactttata ttgtttaaga ttaagaggaa caaagtggct aaaacacaaa gttgcataga
     1501 atatatgaaa tcacttttag atggataact ctatttagaa gtgatttttt atgtagttat
     1561 tatcataatt ataagtatga tatataaggt gattaaatta cataaataaa ggctataata
     1621 gtatggatat tataaattat atattgagca gaattacata atttggcgat aaatattaga
     1681 aatatatatc tatagaaaat aatatatatg ggtatattat taaatataaa aaatattagg
     1741 tatttattta ataataaagg tgggtattgt tatgataaat aaaataggaa aaataactct
     1801 ctatgtaaat aatcaagagg atgcaaagtt attttggaca gaaaaattga actttgtagt
     1861 aacttatgaa aaggaaatgg gtccaaatat gaaatgggtg gaagtagcac caagtaaaaa
     1921 tgaatttaca acatttgtgt tgtatgataa aaatctaatg atgaagcaaa acaaagagat
     1981 aaatgtatct catccatcaa taattttaag tacaagagat attaatggaa cttatgaaca
     2041 aatggcaaat aacgaggttg aggttgggga attaatggag atgccatatg gaaggatgtt
     2101 ttcttttaaa gaccaagatg gaaatgatta tttggtcaga gaggataaat aattttacaa
     2161 agtattaaat ttactcaata tctgatataa ttagagacaa taattattat aaactagtct
     2221 aggattagaa ttatataatt aaaaattaag tgtaaatttt gagtagaggt gataaaatgt
     2281 ttaagaaaat ggcagtacta aaagatatag caactaaaat aggtcgtaaa aaagcgtatg
     2341 aactattaga aatggttgaa ggtaatgatg cctttgtagc tgaggtaaag ataaaaaaga
     2401 atggaataga atctaaaaaa gaagaaatta tgttaaaaga taatcaaaaa ataatattag
     2461 agtatataga aggttaaatt ttaaatacat ataagttgat atactaaatt tctgagatga
     2521 gtttacataa aactcatctt ttttactttt caataacttt gttttattat ttaatataca
     2581 ttttatctcc ttagagttgc tgatatataa aaattactga tataataaaa gaatggatat
     2641 taatgtaata ttttatagaa aagaggggta ttatgcttga aataaaaaat ctttccttta
     2701 atgttgagtc taacaatgag gaactaggaa taataaatga tgttagcctt tcatttgaga
     2761 ggggaaaact tattgttatc acaggtccaa atggtggagg aaaatctaca attgctaagc
     2821 ttattatggg aattgaaaaa gctacgagtg gtcagataat tttagatgga gaagatataa
     2881 caaatcttag tattacagaa agagcaaaaa aaggaatagg atacgcattt caacaaccac
     2941 caagaataaa aggtatgaca gttgaaaatt tacttacact tgctcatgga aaaccattgt
     3001 ctactgatgt atgttgccaa taccttacag atgttggatt gtgttcaaaa gactatttaa
     3061 atcgtgaagt tgataactct ctttctggag gggaaatgaa aagaattgag atagcaactc
     3121 tttttgctag agatttaaag gtttctatat ttgatgaacc tgaagcaggg atagaccttt
     3181 ggagctttgg aaaactcaat gaaagtttta agaaaataca tgaagagtct aatcagacta
     3241 taatcataat ttctcaccaa gaaagaatat tagagttagc tgatgaaata attgttctac
     3301 aagatggttc agtaaaaagc catggaacaa aagaggctat attacctgaa atcatgtgtc
     3361 aagttaattc aagttgtgaa ttaatgaaag acatgaatta attagaaagg aaggcaaatt
     3421 aaatgaacaa aatagattta aatcttttag aaaaagttgc tgaacttcgt ggaactccat
     3481 taggagcata caatatacgt aaaaatgggc aaggagtaga gagacattca actgaaaatg
     3541 taactataat tcctaaaact gataaacctg gaatagatat tatagtaaag ccaaatacaa
     3601 aaggagaaaa tgtacatata ccagtaatag taacagaggc tggtgtacag gatatggtat
     3661 ataatgattt tgaaattgga gaaaatgcag atgttgttat aattgctggg tgtggtatac
     3721 ataactgtgg ttgtgatgat agtgaacata atggtataca tagatttttc ttacgtaaag
     3781 gagctcgact aaaatatgta gaaaaacatt atggtgaagg tgatggagaa ggaaagagaa
     3841 ttttaaatcc agttactatt gtcaatatgg atgaggattc atattgtgaa atggaaacaa
     3901 ttcaaattaa gggtgtagat tcaactaaaa gagaaatgga tgcaaaccta ggtaaaggag
     3961 ctaaaatagt tgtatttgag aaattattaa ctcatggtaa tcaagttgca gaatctaata
     4021 tgaaggttat cttagagggt gaaaactctt ctgctcaagt agtttctcgt acagttgggc
     4081 aagataattc taagcaaaag tttaatcctt gtgttattgg ggattccctt tgttctgcac
     4141 atgttcaatg tgattcaata attatgggaa attctcaaat tagtgctata cctgaaattg
     4201 ctgcaaatca tccagatgct cttttagtac atgaagcagc tattggaaag attgcaggag
     4261 accaaatact aaagatgatg acattaggtc ttactgaaga agaagccgag caacaaatac
     4321 ttaattgctt cttaaagtaa attaaagatt ttatttaatt taatataatt aatatagtat
     4381 aatatagtga ataagtctaa aatattttta tttttaggct tattttcttt atgtaagaaa
     4441 agctattaga gaaattatat tgtaattagg atgtggaata aatgaaaaat ttacttataa
     4501 gaaatgggac aataatatct ccaacagatg gtatcaaatt tgaaaaaaga gatatcttgg
     4561 ttttacatgg gaaaatatat gctattggat tgaaccttaa agagaaaata agaaaagaaa
     4621 aattacttac aaataaagag ttcgagataa taaatgccga taatatgtat atttccccag
     4681 gatttataga tgttcacacc cattgttata agagaaaatt acctacaggt atggattctg
     4741 attcaattgg aattgaaaaa ggttctacag ttatatttga tgcaggtaca gctggtccct
     4801 caaactacta tgattttaaa aaaaagtata tggatgagtg taaaacagaa gtatattctt
     4861 ttttaaatgt tacaaatgag ggactaaata tattgaatga atcaacttat ttagatgtta
     4921 taaattttga caaggttgag tcaattgtag aaaaaaatat agaaaagata aagggtataa
     4981 aagtaaaagc gagtaaattt caatctaatg aaagtggtat agatataatt aagaaatcaa
     5041 agcaagttgc taaaaaatta aatttaccct tagtggtata tataggagag tatccatcat
     5101 atatagatga agtaatgaat attttgggaa aaggagatgt ggttacaccg gtatatggaa
     5161 atagtacaaa tggaatcctt aaagaatctg gcacattaag aaaatcagta ataaatgcaa
     5221 agaatagagg agtattgttt gatgtatgtc atggagtgga ccaatttgat tttaaagttt
     5281 ttaaaaaagc aataaagcaa ggtttagagc ctgacctaat aagtactgat atgcatataa
     5341 aaaacatgaa agacgtaaac tattctcttt taaatgttat aaataaaatt atggaacttg
     5401 ggatatccct agaaaagtgt atagaaaaag taactgaata cccaacaaag attttcgatt
     5461 tacatggaaa taaaggcaga ataaagattg gtggagaagg tgattttaca atatttacaa
     5521 tggaggagtg taatgatatt ataaaagact acaataataa tgaacttgtt ttaaataaaa
     5581 aattaagatt gcaatatact gtaaaatcat ggatgaaaag cagtgaagtt tatagacatg
     5641 gatacgaaaa tactataata aactaaacaa tagtgataaa ataataaata tatgtttaaa
     5701 ttggtatata ttggataaat atctaaggaa acaacaatta aaaaataata attttagggg
     5761 aggtatttta gtatgaaagc atttgttgat aaggatgtat gtgtagcttg tggagcatgt
     5821 ataggaacat gtccagacgt ttttagtatg agcgatgaag ggcattcagt agcaattgaa
     5881 ggagaaatac cagcagattt acaagattta tgtaaagaag ccgaagaagg atgtccagtt
     5941 tcagctataa cagttaaata gagctgttta aatttatttt taaaataaat atataagatt
     6001 taattggtac ttaagctata gtattagaaa tttctattac tatagctttt taaaatagat
     6061 aaacttaata tataagatat tactttttac aaatatggag gactgaaata tgaaaaaaaa
     6121 ttatgaaata gtattattcg atttagatgg aacacttaca gaccctaaag aaggaataac
     6181 aaagtctatt cagtattctt taaatagctt tggcataaaa gaagatttgg aaaacttaga
     6241 ccaatttata ggaccaccat tacatgatac tttcaaggag tattataaat ttgaagataa
     6301 aaaagctaaa gaagctgttg aaaagtatag agaatatttt gctgataaag ggatatttga
     6361 aaataaaatt tatgagaata tgaaagagat attagaaatg ctctataaaa atggtaaaat
     6421 attattagta gcaacttcta agccaacagt ttttgcagaa actatactta gatattttga
     6481 tatagataga tattttaaat atatagcagg tagcaatctt gatggtacaa gagtgaataa
     6541 aaatgaagtt atacaatatg tactagacct atgtaatgta aaagataaag ataaagttat
     6601 tatggttgga gatagaaagt atgacataat tggagctaaa aaaataggta tagattctat
     6661 tggagtattg tatgggtatg gttcttttga agagattagt gaaagtgaac ctacttatat
     6721 tgtcgaaaat gtagagtcta taaaagatat tttgttataa atatctttta tagacaaaaa
     6781 tattacaaaa tggtgtcgca tatttaaaaa tatgatatta tagttaagga agtcatttaa
     6841 aaaagaggag gatgcactta aaatgaaatc tattttaatt atgggtggaa gcgacttcat
     6901 aggttctgca cttgctaagc gcttgattaa atgtgggtat caaattgata tacttacaaa
     6961 tggtaaaaag gaaatagatt ataatggttt taaagaacat ctaatttgtg atagaaaagt
     7021 tcgaaaagat atggaaaaca ttatcacagg aagaaaatat gattacatat atgatatgac
     7081 agcatataca aaagaagatg taagtaatct gattgatttt ataagcatgg ataacttaaa
     7141 aaagtatata gtattgtcag caggagcagt atataaagat agtggaagaa atataaaaga
     7201 agaaaatgag aaaggtgaaa atgaaaactg gggaaaatat gggttaaata aaaaagaggc
     7261 tgaagatttt attataaata gccctatacc atatataatt attagaccaa cttacatata
     7321 tggtgaaaat aataatttat atagagagta ttatttcttt gaaaaaatag aaaaaaatga
     7381 aaagatacca gtaccaaaag gaaaacaagt tagtaatcaa tttatatata ttggggattt
     7441 ggtaaaggta ttggaaagca taatgaaaaa tcctcatgta agagaggcct ataatgtgac
     7501 aaatccacag ttaatttctt gggatgacct tatatataca tgtggagaaa ttattggcaa
     7561 agaacctata ataaaatatg tagatatgga aaaagtagag tttagagaaa gaacgtattt
     7621 cccatttaga aatatagact ttaatcttga tataaataag cttattgaac atgggcttta
     7681 tataccaaac gtgttactaa aagaaggtct tacagcaaca tataaatggt tttcagcaaa
     7741 taagccaaaa atgcatgaca gaaaaatgaa caaagttgag tctgtaatgc aaatagttta
     7801 aaaaaataaa taagtaggta gatattttga actcaaaaat taagtatttt ttaaaatttt
     7861 ttatcttaat atttattttg atttttatat actcagttgc gatagagccg agtctattaa
     7921 ttgtgaaaaa atataatata agtgaaaatg gagcattgat aaaaaatgat tcaattgaga
     7981 gtaaatctga aagtgtaaaa gtagttcaaa tttcagatac tcaaataggt agtttttatt
     8041 caactaagaa tctcaaaaaa gtagctaata agattaacac tttgaatcca gatataatag
     8101 tatttactgg tgatttaatt gattactcca ataaaaatcc aagtgtagat gaaattacaa
     8161 ctattttatc aagtatgaat gctaggttgg gaaaattttc agtatttggc aatcatgatt
     8221 atatgtataa acttcctagg tattataggc agattatgaa aaattccaac ttcaatcttt
     8281 tagtaaatga aaataaaaaa ataaaattaa aagatgataa atatattaat atattaggtg
     8341 ttgatgaaat attaaatgga aatcctaata taaaatatct ggagagtcaa atagataata
     8401 aaagttttaa tttattgtta gcacatgaac cagatttagt agatatgctt agtaaagata
     8461 caatgaattt agttctatct gggcatagtc atggtggtca aataagatta cctattaagg
     8521 gagcattagt tactcctcca tatggaagga aatatactaa agggttttat gacataaatg
     8581 gaaatcattt atatgtgagt tcgggtcttg gaagtacaaa gttaccattt agatttttta
     8641 atataccaga gataatagaa tttaatatta taatctaaca taaaatattt agaaaatata
     8701 attttgcttt ttataatatg tagatgtata tactgtaaaa atggattaaa ccagtagatt
     8761 gaatgaatta atagattaaa ttaatatata aaatatttaa gttataatta atgtattaga
     8821 caaaataaat actattaaga actattaaaa tatagattaa agataataac atataataaa
     8881 taaaaaagta agattgtgta gattatataa agattattta caattaatac aatcttgctt
     8941 ttttatttaa aaattttaat tattatttaa aattttttat cttctcttta tcccatataa
     9001 acaatttctc agaattattt aaagcaccaa atacatttct tttaaaatca ggcatctctt
     9061 tatttaaatt agcaataaga ttttttatgt actctctcct agtatgacca tttgcagggc
     9121 agggatttgt gataatagga tagttagaat cttttgaaat tttcttagtc atatactctt
     9181 ctatgtaaac cataggtctt ataactgtta aatcctgtct gtctaggtat gtctttgggg
     9241 aaaaacaatt tactctaccc tcataaaaca tagacattaa aaatgtctca attgcatcat
     9301 ctttatgatg accaagagct actttattac atccaagttt tttagcatta tcgttcaaag
     9361 cacctcttct taaattagca cacaatgcac aaggattctt ttcttgtctt atatcaaata
     9421 caatttcttt tatgtcagtt tgaaattcat aaaaaggaac atttatttct ttacacaatt
     9481 tatctagtgg agaattatca acacctccag tatttaatgt tatggctatc aattcaaagt
     9541 tttgtggaga aaatctttga taacttttaa gtatatgaag tagagttaaa ctatcttttc
     9601 caccagaaag tccaaccgca attttatcat tttcttgtat catatcaaaa tcttgtatag
     9661 ccttacgagc tttacttaga agtttttgta tagacatttt atcaccatcc tttaaaaata
     9721 ttaaatttaa gatttattta agatttactt ataaaatact taagtcttat actctaaaat
     9781 taacattata gcaaacataa caaaaaggag aagatttaat tatgaaaaaa tttaaattaa
     9841 taggagccat tattttagta gtatttataa gtgtatttat atctaaagat agattactta
     9901 agaaagataa agatccaata gtagctgctc aacatgattc agaacaagat gttgaggcaa
     9961 attctcaaaa taaaaatcac tcaaatagta tatcaaatat attattggta aacaagacaa
    10021 atggaattag caaaaactat actcctgaaa atattactaa ggtaaacata ccttttgttg
    10081 aagaagcaac agaggaagaa aaacaaatgg caggagaacc tgcaaaggca gttgaagatt
    10141 tagttaagca agctaattca gaaggaatac agtttttagg gtcttcagca tacagatctt
    10201 atgatactca actggatact tatacaagaa gggtaaaatc tcaggggcga gaaaaggcag
    10261 atgcgtatgt agcaaagcct ggttacagtg agcaccaaac tggactttgt attgatttaa
    10321 caaatccaga aagatggttt gttggaagta caaaagaagc taaatggttg gcagaaaatg
    10381 ctcacaagtt tggatttata ataagatatc ctgaaggtaa agaagatata acaggtacag
    10441 catatgaacc atggcatatt agatatgttg gtaaggatgc tgccgaggaa atttattcaa
    10501 aaggattaac tttagaagaa tatttacaaa ataaataata taaatgtgta aataatttgt
    10561 attatattgg aaatttagaa attaataaat ttatagatta cactaactaa aatatagtat
    10621 aattttatat gaataaagct atatacaaag attaagttag gatgtgtaaa aatgaataat
    10681 acagttcttg ttgtggatga tgaaaaggaa ataagagacc ttatagagat atatctttta
    10741 aatagtggat ataatgtaat taaagctgaa gatggaaaag aggctctaga tattttaaaa
    10801 aaggaaaata ttcatttaat gatattggat ataatgatgc ctaaaatgga tgggatagaa
    10861 gtttgtagaa aagttagaga aagtttgaat ataccaatac ttatgttaag tgcaaaatca
    10921 gaagacatgg ataaaataca agggataatg actggtgcag atgattattt gacaaaacca
    10981 tttaacccac ttgaacttac tgtgagagtg aagtcgctga ttagaagagc atattatttt
    11041 agtggtgcta ataatgaaga taaagatgtc ataaaggttg gagtagtaat tatagataag
    11101 gaaaaacata gtgtaacagt ggctggaaat gaaataattt tgacatcaag agaatttgat
    11161 atattatatt tactagcaaa taataaaggc agagtatata gtacagaaga aatatttgaa
    11221 aaggtatgga aagagaaata ttaccaatct aataatactg taatggttca tatgagcaga
    11281 ataagggata aaattgaaaa atatacagat ggagaaaaaa taattcatac tgtatgggga
    11341 gtaggatata aaattgaaaa ataaaagttt attaaatcat atgtttcgac ccatattaga
    11401 tcttataata agctgtgctt taacttcaat gcttatttta acaagtatgt atataataaa
    11461 atcattatat gtcaatagtg atatgtctgt agttaatcaa cttgtatatc aagcaagaga
    11521 tttcagaagt cttatagggt atgatttatt ttatccattt ttatttacaa tcacatcatt
    11581 agcaatttat tccatgatat cgtacaagag aaataagcaa attgtagatt ttataagaaa
    11641 gactaataga aaaaatcaaa aattagaaat cattaaaagt gctgtaaatt taatggatga
    11701 aggtaactta gaaaaaagta tagatattga gtatgattta gatatattaa aggataaaga
    11761 aaaggatgat attgatgaac tagcacataa aatcaataat atagtccatc aacttagaaa
    11821 tataactatt gaagagagac aagctcagca gacaaaaaca gatttaataa caaatgtgtc
    11881 acatgactta agaacacctt tgacatccat aatgggttat ttaggagtga tagaagaagg
    11941 taagtataaa gatgaagttc agatgatgta ttatgttgat attgcatatg aaaaatcgaa
    12001 aaatttaaat gttcttataa atgatttgtt tgaattgact aaaatgcaaa ataatacaat
    12061 aaaattaaac aaaatagaaa taaatttagt agagttatta agtcaagtag tatctcaatt
    12121 tgaaatttat tttaaacaag aatttatggt tagtagaatt aattttaatg aagaaaaatt
    12181 aattgtaaaa gctgatccaa ataaattggt aagagcattt gaaaatctta taactaatgc
    12241 tgtgaaatat ggaaaagatg ggcattatgt agatatagtc acagagaaaa aggaagatat
    12301 ggcggttgta aaagtgataa attatgggga accaattcca gtgttagatt taccaaatat
    12361 atttgataga ttttatagag ttgaaaaatc gagaaataga aatgttggag gttctggatt
    12421 gggtcttgcg attacaaaaa atattgtaaa tttacatgat ggagaaatta ctgtttcaag
    12481 cgataaatat caaactgtgt ttgaagtaca actgccaata atacaatcag cttagttata
    12541 aaataaaagt acgattatgt gatataaata ttataacaat aaagtgttta caaaaataaa
    12601 aaagtaatat ataatttaag tcagtttaat aagatactta tcaagagtta ggggagagag
    12661 ttagctcagt gatcctatac caacctttta tcaggtggtc ctgctaacaa cgatgagatg
    12721 acttgaaata atatactctt cttatcaata atagatagga agagtttttt ttagaattaa
    12781 tgtaacaaat tttaaagtat aatggaggat taaataatgg aaaaagtaga aagttttaag
    12841 ctagaccata caaaagtaaa agctcctttt gtaagaaagt gtagtgtact tgatggagta
    12901 aagggagata aagtaactaa atttgacttg agatttttac agccaaatgt agaaagtttt
    12961 ggaactgctg ctatgcacgg tttagagcat cttttagcta cttatttaag agatacatta
    13021 gatggtgtaa tagacttatc tccaatggga tgtagaacag gattttattt aatactatgg
    13081 ggagatgtag atgccaaaac agttaagatt ggtttagaag aagcattaaa aaaagttctt
    13141 gaatcagata aaatgccagc agctacagct atagaatgtg gaaactacag agacttatct
    13201 ttatttgggg caaaggaata tgcaaaagat gttttagata aaggattttc tttaaatata
    13261 tatggagaat aatttaattg gaagagaaga tataagcata aatcaaagtg aagttttaag
    13321 atatttacaa tatagaggtc aagagataga taaagacctt aaatatacta ttgatgaatg
    13381 tgtagctata actaaaaata aaataaatcc tagatattta tctaggattt atcctattaa
    13441 aataaataaa gataatgttg tagaattaga gggaacaaat ttaacattag agagtaaaga
    13501 tgtatatgaa cttttaaaag aatgtaatga gtgtgtaatt atggcagcta ctatagggat
    13561 agatatagaa aaagaaataa gacgacactc ttattcaaat ttaacaaaag gactaataat
    13621 tgattcatgt ggaacaacag caatagaaga agtgtgtgac attgttcaaa ataatttaga
    13681 aaaggttttt ttaaagcaag ataaatttat aactatgcga tatagcccag gatatggaga
    13741 tttaccaata gaaaaaaata tagatatatt aaacgttttg aatgctcaaa aacaaatagg
    13801 tctaacaatt acttctaatg gaattatgat accgagaaag tcagtagtgg ctattatagg
    13861 tatttctaat acaagaataa ataaatctaa gaaagaaaag tcgtgtgaaa attgtaaaaa
    13921 ctatagaaac tgtacatata gaaaaggagc gaactcttgt ggaaataaga caatatctga
    13981 aaaataatat attaatattt gatggtgcaa tgggaacaat gcttcaacaa aaagggttaa
    14041 agttaggtga aaatccagaa gtatttggtc ttcaaaatcc agataaactt atagaaatac
    14101 atacagcata cttagaggca ggttcgaatg taattcttac aaatactttt ggatgtaatg
    14161 aactaaaatt agatggtaag tatactgtag aagaagtgat tgacaatgct gttttggtgg
    14221 ctagaaaagc tatagaaaat gtagataata ctaaaccaag atatgtagct ttagatattg
    14281 gtcctatagg agaaatgcta gagcctatgg gtactttaag ttttgataaa gcatatgaaa
    14341 tatttaaaag acaagtacta caaggcgtta aatctggagt tgatgtaata gtaatagaga
    14401 ctatgatgga tttatatgaa gctaaagtag ctgtattagc agcaaaagaa aactctgatt
    14461 tacctatttt ctgtactatg acatttgatg aaggtggaag aagttttact ggatgtatgc
    14521 cagaatgtat ggtggctact atagaaggtc taggtgtaga tgcaatagga gttaattgtt
    14581 cattaggacc taaacaactt ttaccaatag ttgaaaaaat agcatcaaga gctacagtcc
    14641 ctgttatggt tcaagcaaat gcaggtcttc caaatatagt tgatggagag gctatatatg
    14701 atgttgatgc aaaagaattt tttgaagggg ttaaaaagtt cgttgaagtt ggtgctacta
    14761 taataggtgg atgttgtggt acgaatccaa gttttataaa agaaatatct gagaatataa
    14821 attctgttac aaagggatgt atcgagaaaa ttgataaatg tgtagtctgt tcaccatcaa
    14881 aatttgtgga agtacaatcg cctacagttg ttggagaaag attgaatccc acaggtagaa
    14941 aatctctaca agaagcatta aaaaatgaaa atgttgatta tgctataaat ttaggtttag
    15001 aacaagtaaa tgctggagca caaatacttg gagtaaatgt tggtcttcca gagattgatg
    15061 aaaaaaaact aatgccaaaa ttaataagag agattcaagc agtagtagat acacctttac
    15121 aagttgattc ttccaatgta gaggccttag agcaaggact tagatattat aatggtagaa
    15181 caatagtaaa ctctgtaaat ggcaaagagg agtctttaga aagtatatta ccaatagtta
    15241 aaaagtatgg ttcatgtgtg gttggactta cacttgatga aaaggggata cctaaaaagg
    15301 ctgaagaaag atttgaaata gcaaaaagga tagttaatag agcagtttct tatgggataa
    15361 aaccaaaaga tatatttata gactgtttat cattaactgt ttcagcacaa caagaagaag
    15421 caatagaaac tataaaagct ataacaatgg taaagacact aggagtaaaa actatattag
    15481 gtgtttcaaa tatttcattt ggtcttccaa ataggaaagc acttaatgct tcttttctaa
    15541 cattagcttt aggggcaggg cttgatttag caattataaa tccaaatgaa tatagtatga
    15601 tggaagctat caattctttt aaaatattaa ataacacaga taaaggatgt ataaactata
    15661 taaatcaata tagcaatata aataattcaa aaaaatcgga ttcatctaca aagattgata
    15721 aagatttgcc cttggatatt ttagttgaaa gaggattaaa agatgaagca aaaaatgtaa
    15781 cactgaatct tttaaaagag aaggatgaaa attatatatt agatgagatt ttaattcctg
    15841 ctcttgataa agtaggaaaa agatatgata gtggagatat atttttacca caattgattc
    15901 aatcagcaga aacggtaaaa gtttcattaa atacaataaa ggaaacttta ttaagtaaaa
    15961 gtagcaataa tgtatccaaa ggaaaaataa tagttgctac agtaaaaggt gatatccatg
    16021 atattgggaa aaatatagta aaaataatgc tagaaaatta tggatatgaa gttatagatt
    16081 taggtaaaga tgtccctatt gaagaagtag tggaagttgc taaaaagaga aatataaaat
    16141 tagtaggatt aagtgcactt atgactacga ctgttcaaag tatgaaagat acaattcagg
    16201 cactgagaga taatgagata aatgcaaagg tatttgtagg aggtgcagtt ttgacagaag
    16261 aatacgctga agaaatgggt gctgattatt attcaaagga tgcaaagtct gctgttgaaa
    16321 tagcaaagtt aaatttttag catataaatt aaaataaagg ttttactaaa taggatattt
    16381 tatatataat ataaatataa gttttagata cttatgttta aaacaatagg aattaatcta
    16441 taataatgag attaattaat ataaaaaata tttatcatca acattaaaaa gggctgggaa
    16501 gtctggtata acttccatca gcccttttta gttaaaatca tatgttaaaa agcccaatta
    16561 ccatttttaa aaatctgtat ttgctttcca tcataagtct caccaataat atccatatca
    16621 gcagaaccaa tcataaaatc tacatgtgtt aaactgtcat ttacaccatg ttcatctaat
    16681 tcttcatctt ttaaattgtt tccaccttcc agacaagtct tataagcaga accaattgca
    16741 aagtggcaag aagcattttc atcatataga gtattataaa aaattatatc cgtattggat
    16801 attggtgagt tgtatggaac aagagcaact tctcctaaat aatgagagcc ttcatccgtt
    16861 tcaataagtt tttctagtgt atctagtccc ttttcagcag agaaatctat tattttacca
    16921 tctttaaatg ttaagaagaa attgtcaatt atgtttccac cataaacaag aggttttgta
    16981 ctataaacag ttccatttac tttaaactta tgtggcattc caaaaatctc ttcagttgga
    17041 atattcggat taaaacttat tccattaggg tctttggatg ctccacttag ccaaacatga
    17101 ccctttggca agtctagagt caaatcagtt tttgaggatt tatattttaa agttttaaag
    17161 ttatttttat ttagaaagtc cattttagat ttaagatttt cattatgttc ttcccatgct
    17221 tttatagggt cctgaccatc tactcttgaa catttaaata tagcatccca cagtttttct
    17281 attgctttat cttctgataa atttggaaac actttttttg cccaatcttt tgttggcaca
    17341 gagactatac tccatttaca tttatcagtt agagtgtaag aacgccattc ttttaaagct
    17401 tttccagatg ccttctgaaa atttgcaact ctttcagggt caacatcttt taataaatct
    17461 ggattttggg catagactga taaaaatgcc ccaccttctt ttgctatatc tacatattgt
    17521 tctgctgtcc atttggggaa ctcatgaaat gcttcatctg gagcatataa gtatttaata
    17581 agagtacttt cttcatcaga ccactctata taaacatttt tagcaccaca tttatatgct
    17641 tctttaagtg catttcttgc aaaatcagca cattctattg gtgactttat aagtagaatt
    17701 tgacctggct gtatgttaac tccaactttt attgctaatt cagcgtattt ttctaaattt
    17761 ctatttaaat tcatatttat cctcctatat ttttaatatt gaataactta taaagtatta
    17821 taacattact tatgttttta atattgaaca acttataaaa tattataact ttacttagat
    17881 tttgggtcat attcaaaata tttatacttt atagattgag aaataaaaaa aatattttaa
    17941 ttaaaacaaa ttatgaggat aacaaactta tttaagtagt atgttttttg ttgataataa
    18001 tattaaagta gaaataaaaa tactttttta gtgaaaagct atacaagaat accgaaaaaa
    18061 acttataata taataatgag atagatattt ataaatgaaa aaaaattttt ctggcaaagc
    18121 ttaattaaaa aggtaatagc tagtgttgtt atttaaatta gcatagaaaa tcaaataaaa
    18181 aaattatgct aatgtataga cattgataag gtagaattta agccttttga atgctatatt
    18241 aaactagagt agatattctt atgaatttca aaatagggca ggaggaaaaa ggaaataaaa
    18301 ctataaaaaa tataaagtat ttatataaat ttatagagtt tatacgtcag aagatttcag
    18361 ttaggagggc ataaatggag ctttatgaag ccatttttta tagaaaatct gtaagaaatt
    18421 tttccagtaa aaagataaaa aagccattga tggaagaaat aaaaagaagt tgtaaaaatt
    18481 tagaatactt aaatgaagat ttaaatatta aagcacatgt tgttgataga ggtcatataa
    18541 tacatttttt gatggggaag gaatgtaaag taaaggcacc tcattatata gtagttacat
    18601 cagacaaagg tgaggactat ttacagaata taggatttgc tacagaaggt gtagttttgc
    18661 gccttacaac attgggaata gctacttgct ggttggaagg aaacttaaag agagaagata
    18721 tcttggaatt tgtagatatt gggaataaga attttgaaga agatgaggaa gatgatttag
    18781 attatctagc tggaataaaa acagaagaag atgatgaaaa caaaatagaa aatccttata
    18841 taataatagc atttggatat ccagcaaaaa atgaagagtt gtttaggacg agatatgcta
    18901 gacctgatag aaagccagta aaagatatga taaataagat aaataggaat agaaagtgga
    18961 aagatatctt agaattgaca agaagagctc catctgtaaa aaattctcaa ccgtggtatt
    19021 tccataagga tgaacgaggt cttcatttat ttgaaaagag acctaagaaa cactgtgaag
    19081 atatgaataa ggttagctta ggtgtagcct taagacattt tgatatagct tgtataaaaa
    19141 ataaaataga tgtttcatat gaaaaacttc ctataaggaa taaaatagga aaatcatatt
    19201 ttataactgt agtcgagcat gtgaaaccag aagaagaaac ccaagaagaa aatgtaactc
    19261 tagaaaaaga agaatcgcaa gatgaataaa attttggaag gggatattgg atatgtataa
    19321 gtgtggaaaa tgtaatctta gaggctgttc taatggaaat atggataaat taatgaagat
    19381 atgtccaact agagaaattg aactacaaca aaaagcaaag caactttatg aagaagaaga
    19441 gaatagaaaa atagcttata atgctggatt agttgaagca gaggggtatt gtcaatatac
    19501 tagactaaaa gagattatag agtttgctaa aaagtgtggt tataaaaaga ttggcatagc
    19561 attttgtatt ggactaaaaa gagaaatgca aacagtgcaa aaaatatttg aacatcatgg
    19621 gtttgaagta ttttctgtta tatgtaaaaa tggggcaatg cctaagagta caattggact
    19681 agaagcatct caaacaatat caaagtgttc agatgaggta atgtgcaatc caataggaca
    19741 agctttattt ttaaatgaag ctaaggttga ccttaatgta attcttggat tatgtgttgg
    19801 tcacgatact ttagccatga aatatatgga agctcctata acaactttag ttgtcaaaga
    19861 tagagtaact gggcataatc cagttgctgc aatatataat gcagaatcat attttgagaa
    19921 aaaattgttt gataaagatg aagaataatt caaagactta ttgacataaa aagcaattag
    19981 tggtttaatt ataaaaaaga taaaacttta aattatccta tagatacata aagttatata
    20041 aataaagaaa aatattacag ctaaattcct taaagtagtc cagagagact aaagggttat
    20101 agataatgtt agtatataac ctctagtaaa tcttctagag gtttttgatg agtataaaat
    20161 aaaaatttaa atataataaa gtcttttaaa ctagtccaga gagactggga aagatatctc
    20221 atagctaatg aatagagtta tgtatagata aaatccttat gcctcttttg gtgtgaggat
    20281 ttttttatga ttatttaggc tttattaaaa aggagatgta aaaatgataa atggtaaaga
    20341 aaaattgata gttgcaattg atactgacga atttgataaa gctaaagaac taatagacag
    20401 attagaagat agtgtagaca tatttaaggt agggctagag caatacgttg ccacaaaagg
    20461 taaaacaata gattatctta aagaaaaagg gaaaaaaata tttttagacc ttaaattcca
    20521 tgatatacca aatactatga aaagtgcagt gaaagctgca gtgagagata atgtttggtt
    20581 aatgacaata catgtatcag atatggaagg aatgagacaa tgtgcactaa ttgctaggga
    20641 agaagcagaa agattaaata tagaaaagcc attagtagtt ggagtaacag ttttaacttc
    20701 attaagcaac caagatttac aggacatagg atgtaatatg acaacagaag agttagctat
    20761 aaagagagct aaactggcaa aagaatcagg aatagatggg gttgtttgtt cagcgcaaga
    20821 agtagataaa atagtagaag catgtggaac tgattttata acagtttgcc caggaataag
    20881 acctaaatca gcagaggttg gagaccaaaa aagagttgta actccatcag atgcaataaa
    20941 aaaaggtgct cattatttag ttgttggaag acctataaca aaagcagaaa atccaagaga
    21001 atcagctatt aatattgtaa gagaaataga aaatgcttag ataaaatatt tgggaggtag
    21061 cttacatgaa ggggaaatta attttagaag atggaacagt atttgaagga aaagcatttg
    21121 gatatctaaa ggaagctatt ggagaagttg tatttaatac ttcaatggct gggtatggag
    21181 aactattgac agatgcaact tattatggac aaatagttac tatgacttat ccacttatag
    21241 gaaactatgg aataaattta gaagatgttg aatcaaataa ggtgcaagta aaaggactta
    21301 ttgtaagaga aaaaagtgat aacccaaata attttagatg tgaaatggat atagacacat
    21361 atctaaagca aaataaggta ataggacttg aaggaataga tacaagagca ttaactaaaa
    21421 ttattagaaa caatggaact aagagaggaa ttataacttt agaaaatgta tcattacaag
    21481 atgttcagtc taaattagaa gagcattgta acaaagattc agtaaaaaat gttacaagaa
    21541 aagaaattga aaatataaaa ggtactggag ctaaagttgt agttatagac tttggtgtta
    21601 aaaatagtgt actagagtct cttaaggctt gtaactgtga tataactata gtaccagcaa
    21661 tgactacagt agaagaaatt ttaaatataa atcctgactt aatcttatta tctaatggcc
    21721 caggaaatcc agaggatata aatgatatag ttgaaaatat aaaaaaatta ataggcaaaa
    21781 agccattgac aggaataggt ttaggacatc aaattttagc attggcatta ggaggaaaga
    21841 cttcaaagct tgtatttgga catagaggag gtaatcaccc agttaaatgt ttagaaactg
    21901 gaaaggtatt tataacttct caaaatcatg gatataatgt ttcagaaatg ccagaaaata
    21961 cagaagtaac tcacataaat ttaaatgaca atactataga aggaatgaga cataaggaat
    22021 taccaatata tagtgttcaa tacattccag aatacacaac aaaagaagat ttagattgta
    22081 tatttaataa attcttagca ttaatataga tttagtatta tttacagata gtaaaaacca
    22141 tttagataag gagaaaaatt tatgcctaaa ttagatagta taaagaaaac tttagtatta
    22201 gggtcaggac caataataat aggtcaagct gcagagtttg actattcagg aacacaagct
    22261 tgccagtctt tgaaagagga aggaattgaa gttgtattaa taaatagtaa tccagccact
    22321 ataatgactg ataaagaagt ggcagataaa atatatatag aaccattaac aatagaattt
    22381 atagaaaaaa taatagaaaa agaaagacca gacagtttac ttgcaggtat gggtggacaa
    22441 acagggctaa acctagcagt tgaactttat gaaaaaggca tactagataa atatggagta
    22501 aagataatag gtacttcagt agaatctatc aaaaaaggtg aagatagaga tatatttaga
    22561 gaagtaatga aagaaataaa tcagcctgta atagttagtg atatagttac agatttacaa
    22621 gcaggtcttg attatgcact tacaattggt tatccagtag ttgtaagacc agcatacaca
    22681 ctaggtggaa ctggtggggg tatagctgat aatgaggaag aattaagaga aatactttca
    22741 catggtctac aattaagtcc tgttggacaa gtattaattg aaaagagtat aaaaggttgg
    22801 aaagaaatag aatatgaggt aataagagat tcaaagggaa attgtatagt tgtatgtaac
    22861 atggaaaata tagacccagt cggagttcat acaggagaca gtatagttgt tgctccaact
    22921 caaactttaa gtaacaaaga atgtgcaatg cttaaaaaag catctttaga tatattaaat
    22981 gcagtagaag ttcaaggtgg ttgtaacgtt caatttgcac ttaatccaca tagttttgaa
    23041 tatgcagtta tagagataaa tccaagagtt agtaggtcat cagctttagc atcaaaagct
    23101 acaggatatc caatagctaa agtttcgtca aaaatagctt taggttatac tttagatgag
    23161 atagaaaatg cagtaactaa aaaaacttat gcttgttttg aaccaactat agattatgtg
    23221 gtggcaaaaa taccaaaatg gccttttgat aaatttaaaa aagcaaatag aaaattagga
    23281 actaagatga tggcaactgg tgaaattatg agtataggaa gtaactttga ggctgctatt
    23341 cttaaaggga taagatcact tgaaacagga aaatattcat tagttcacac accatctgag
    23401 gagagaagca ttgaggaatt aaagaaaaga gttgtagtac ctgatgatga gagactattt
    23461 gatttagctg aaatgataag aagaggctat aaagtagaaa tgatagaaca aattacaggt
    23521 atagataaat ggtttataaa taaatttaaa tggatagtag agcaagaaga aaaattaaaa
    23581 gttatgaaaa ttgaagattt aactaaagac tatcttcttg aattaaagaa aaagggattc
    23641 tctgataaag gtatatctga cctaatgaag ataagtccag aaaaattata tgaattgaga
    23701 agtctataca atataaaacc agcatataaa atggttgata cttgtgcagg tgagatagat
    23761 gctatatctc cttactatta ttcaacttat gagcaatatg atgaggtagt tgttagtgat
    23821 aagaaaaagg tgatagtatt aggttctggt ccaataagaa taggtcaagg tatagaattt
    23881 gactattgtt cagttcactg tgtaaaatct ttaagaaaaa tggacataga aactataatc
    23941 ataaacaata accctgaaac tgtaagtaca gactttgata catcagataa gctgtatttt
    24001 gaaccattaa cagaagaaga agttttaagt ataatagaaa aagaaaagcc agaaggtgta
    24061 atactacagt ttggtggaca aacagctata aaattggcta aatttttaca tgaaaaaaat
    24121 atacctatat tgggaactga ttttgatgat atagatgcag cagaagatag agaaaaattt
    24181 gatgatttac tagagagact agatataaat agaccaaaag gaaagggtgt atggtcactt
    24241 aacgaaggta ttgaagttgc taatgagtta ggttatccag tattagttag accatcttat
    24301 gtacttggtg gtcaaggtat ggaaataact tataacgaag aaaaattatc tcaataccta
    24361 caagatgcat ttgatagaga ccataaaaat ccagtactta tagataaata tctaactggt
    24421 agagaaatag aagtagatgc tatatgtgat aaggaagata tattgatacc tggtataatg
    24481 gaacatttag aaagagctgg agttcactct ggagatagta caacaatgta tccaagtcaa
    24541 aatatatctg atgaaataaa agaaaagata gtagaataca ctaaaaaaat agctttagac
    24601 cttaatgtgc ttggtatggt taatatacag tttatagaat ttcaaaatga attatatata
    24661 attgaggtta accctagagc tagtagaaca gttccgtata taagtaaagt aagtggagta
    24721 ccaatagttg acttggctac taagtgtatg ttaggggcta aattaaagga tttaggatat
    24781 ggtacaggag tttacaaaga gccaaaatta gtatcagtaa aagtaccagt attctcaatg
    24841 tctaaattat ctaaagttga agtaagtcta ggacctgaaa tgaagtctac aggagaagtt
    24901 ctaggtgtag gagaaaattt agaagaagcc ttatataaag ggttcttggc agcaggtaga
    24961 catatgtctg atgaaagagg agtagtactt gctactgtaa ataatcatga taaagatgag
    25021 tttatagaga tagcaaaaga tatgaaagaa ttaggatata cttttgttgc tacagaagga
    25081 acagctaata gcttaagaga aaatggaata gaggctgata tagtaaatag agtagaagaa
    25141 tctagaccaa acatattaga tgctataaga aataaacaag ttgatatagt aataaataca
    25201 ccaactaaag gaaatgactc tacaagagat ggatttaaga taagaagaac agctattgaa
    25261 ttttctactg aaataatgac atctttggat acactaaaag ctttagtaga agttaagaag
    25321 aaacatttaa ataaagatga gttaaaagtt tacaatatag ctgaatagaa attaagttga
    25381 ttgtatagtt taagaattta attattaaat aaaaaaaaga ggttattttt tctaggagtt
    25441 tttacttttg agataacttc ttttttttaa gaaatttgta gtataataga aaaagataaa
    25501 aaataaatgt aaataagtat aaaactagaa tgtaaaagtg aaaaatagat atagatattt
    25561 aaaagcttat atttagttaa tgttctttag tttgtctaaa gagaattata ggaggtactg
    25621 tagtatgaaa gcaaggttaa tattagaaga tggaacagtt tttataggaa aggcatttgg
    25681 atatttagaa gaaagtgtag gagaagtagt atttaatact tcgatgatag gatatggtga
    25741 agttttaaca gacccttctt actatggtca aatagttact atgacttatc ctcttgtagg
    25801 aaattatggt ataaatttat catcagcaga gtctgaaaaa gttcaagtaa agggattcat
    25861 agtaagggag aaaagtgatt ctccaagtaa ttttagatgt gaaattgata tagaccaata
    25921 tttaaagcaa aataaagtta tcggacttga aggaatagat acaagagctt tgactaagat
    25981 acttagaaat aatggaacaa tgaaaggtat aattacatta gaagacagta aattagaaga
    26041 tgttaaacat aaattggata agttttcaaa tacagaagca gttagaactg ttacaagaaa
    26101 agaagtagag catataaaag gaaatggtcc aaaagtggct gttatggatt ttggagtaaa
    26161 aagaaatata ttaagatcat ttatagctcg tggatgtgat ataacaatat ttccagctac
    26221 aacatctcca gaagatgtat tgagtataaa ccctgactta atattcttgt caaatggacc
    26281 tggagaccca gaagacttag aagatgttat agaaaatata aaagctctta ttggtaaaaa
    26341 acctatagtt ggaatatgct taggacatca acttttagct ttagctcttg gaggaaaaac
    26401 tgctaaactt aaatttgggc atagaggtgg aaatcatcca gttaaagatt tagaagaagg
    26461 aaaagtattt ataacttctc aaaatcatgg ttattatgtt tcagaagttc ctgaacaaat
    26521 gaaagtgaca catataaatt taaatgataa tactgttgaa ggaatgcgac atgagaagct
    26581 agatgtatac agtgttcaat accatcctga agcttgccca ggaccaaaag ataatgacta
    26641 tatttttgat aaatttttag aattagtaaa ataaacaaca ttagaatagc taactaaaaa
    26701 acaagaataa cttaaatcaa aaagtttggt ttaattaaca tagatgtatt aaatagatgt
    26761 attgatgagt aaatttgaac ttaagataaa ataaagtaat taaagttgat tgtatagatg
    26821 atgaagataa gaactgtgaa aattgtttag ataaggagaa agagttatgc ctaaattaga
    26881 tagtataaag aaaactttag tattagggtc agggccaata ataataggtc aagctgcaga
    26941 gtttgactat tcaggaacac aggcctgcca agcattaaaa gaagagggaa ttgaagttgt
    27001 attagtaaat agtaacccag ctactataat gactgataaa gaaattgcag ataaaatata
    27061 tatagaacca ttaacaatag aatttataga aaaaataata gaaaaagaaa gaccagacag
    27121 tttacttgca ggtatgggtg gtcagacagg attaaactta gctgtagaat tacatgatgc
    27181 tggtatatta gataaatata atgtaaaagt tataggaaca tctatagaat ctataaaaaa
    27241 gggcgaagat agagatttat ttagagaagt aatgaaagaa ataaatcaac cagtaatagt
    27301 tagtgatata gttacaaatt tagaagcagg tcttgagttt gccaataaga taggatatcc
    27361 agtagttgta agaccagcat atacgctagg tggaactggt ggaggtatag ctgatactga
    27421 ggaagaatta agagaaatac tttcacatgg actacagcta agtcctgttg gacaagttct
    27481 tcttgaaaag agtataaaag gttggaaaga aatagaatat gaagtaatga gagatggaaa
    27541 tggaaactgt ataactgtat gtaatatgga aaatgtagac ccagttggag ttcatacggg
    27601 agacagtata gttgttgctc caagtcaaac tctaagtgat gaagagtatc aactacttag
    27661 aaaggcatca atagacataa taaatgcaat agaggttcaa ggtggatgta acgttcaaat
    27721 agctttaaac ccacatagtt tagaatatgc gataatagaa ataaacccaa gagttagtag
    27781 gtcatcagcc ctagcatcaa aagctacagg atacccaata gcaaaagtag cagctaagat
    27841 agctttaggt tatactttgg atgagataga aaatgcagta actaaaaaaa cttatgcttg
    27901 ttttgagcca acattagatt atgtggtagt aaaaatacca aagtggccat ttgataaatt
    27961 tagacatgcc aatagaaaat taggaactaa gatgatggca actggtgaaa tcatgagtat
    28021 aggaagtaac tttgaggctg ctattcttaa aggtataaga tcacttgaaa caggaaaata
    28081 ttcattggtt catgctccat cagaaggtag aacattagaa gaattaaagg ctagagtagt
    28141 agtacctgat gatgagagac tatttgactt agctgaaatg ataagaagag gctataaagt
    28201 agaaatgata tcagaaataa ctggtgttga taagtggttt ataaataaat ttaaatggat
    28261 agtagaacaa gaagaaaagt taaaagttct aaaaatagaa gacttagata aagaatattt
    28321 acatgaatta aagaaaaagg gattttctga taaaggtata tctgacttaa tgaagataag
    28381 cccagaaaaa ttatatgaat tgagaagttt atataatata caaccagttt ataaaatggt
    28441 tgatacatgt ggaggagaat ttgaagctct atcaccttat tactattcaa cttatgaaca
    28501 atatgatgag gtagtagtta gtgataagag aaaagttgta gtattgggtt ctggtccaat
    28561 aagaataggt caaggaatag aatttgacta ttgttcagtt cactgtgtaa aatctttaag
    28621 aaaaatggga atagaaacta taattgtaaa caataaccct gaaactgtaa gtacggattt
    28681 tgatacatct gataagttgt attttgaacc attaacagaa gaagaagttt taaatataat
    28741 agaaaaagaa aaaccagaag gtgtaatact acagtttggt ggacaaacag ctataaaatt
    28801 ggctaagttt ttacatgaaa aaaatatacc tatactggga acagacttta gagatataga
    28861 tgcagcagaa gatagagaaa aatttgatga tttactagag agactagata taaatagacc
    28921 aaaaggaaaa ggtgtatgga caactaatga aggtgttgaa attgctaagg agttaggtta
    28981 tccagtatta gttagaccat cttatgtact tggtggtcaa ggtatggaaa taacttataa
    29041 cgaagaaaaa ctaattcagt atttggatga tgcatttgat agagaccata aaaatccagt
    29101 acttatagat aaatatctaa ctggtagaga aatagaagta gatgctatat gtgataaaga
    29161 agatatattg atacctggta taatggagca tttagaaaga gctggagttc actctggaga
    29221 tagtacaaca atgtatccaa gtcaaaatat atctgatgaa ataaaagaaa agatagtaga
    29281 atacactaaa aaaatagctt tagaccttaa tgtgcttggt atggttaata tacagtttat
    29341 agaatttcaa aatgaattat atataattga ggttaaccct agagctagta gaacagttcc
    29401 atatataagt aaagtaagtg gagtaccaat agttgacttg gctactaagt gtatgttagg
    29461 agctaaatta aaggatttag gatatggtac aggagtttac aaagagccaa aattagtatc
    29521 agtaaaagta ccagtattct caatgtctaa attatctaaa gttgaagtaa gtctaggacc
    29581 tgaaatgaag tctacaggag aagtcctagg tgtaggagaa aatttagaag aagccttata
    29641 taaagggttc ttggcagcag gtagacatat gtctgatgaa agaggagtag tacttgctac
    29701 tgtaaataat catgataaag atgagtttat agagatagca aaagatatga aagaattagg
    29761 atatactttt gttgctacag aaggaacagc gaagagttta agagaaaatg gaatagaggc
    29821 tgatatagta aatagaattg aagaacctag accaaacata ttagatgcta taagaaataa
    29881 acaagttgat atagtaataa atacaccaac taaaggaaat gactctacaa gagatggatt
    29941 taagatgaga agaacagcca ttgaattttc tactgaaata atgacatctt tggatacgct
    30001 aaaagcttta gtagaagtta agaagaaaca tttaaacaaa gataaattaa aagtttataa
    30061 tatagctgag taaaagtgat aagtttgcaa agtggtaaat ttgttctata agaggttata
    30121 aattttgtat aacaaaagac tgtagataaa ttttatattc tatacgagtt tatttatagc
    30181 catttataaa aattgtatag tatttaaatc attttaagat aaagggcatt agctatagaa
    30241 gctattgcct tttattttaa taaatggata aatttaattt ttcattgatg tatgaatata
    30301 agcttgtaag atgtgtcatc aagaaagtag tgttagtagg agtgtatgtt tataagttag
    30361 tgagttaaaa aacttgcact tacattatgt tttttgtctt atcttgtgta tttatgttat
    30421 aagaaatata ataaatcaat ggaggtatta tgcatggatt atataagaac aaaagaaact
    30481 actgaaaatt gtgtaatgat aagtaaaata gtagtgtact attgctctgc tggaatatat
    30541 aaaactaata aaataaagga ggatgaaagt aaatgaaacg gatattttta gttgaggatg
    30601 ataaagcaat tgccaaaaat cttattcttt tgcttaattc agaaggattt atagtaaccc
    30661 atgtatccac aagaagtgaa gcacttgtag agattgctaa gaataaattt gatttggcac
    30721 ttattgatat ttctttacct gatggaaatg ggtttacgat ttgcacagaa atcaaagaaa
    30781 agcaagacat tcctgttatc tttttgacag cttccggtga tgaatcaagt gttgttactg
    30841 gactgaatat gggtgcagat gattatatta ctaaaccttt tcgtccacgt gaactgattg
    30901 cgcgaattag aacagcacta cgaaaaagtg ggcgttttgg gtcaactttt gaaatttgtg
    30961 ggctttatgt agatacagca actggtgttg tgaaaaagaa tggcaatgag gtttttcttt
    31021 cagctttaga ataccgtttg ttattggtgt ttattagcaa tcctaaaagt attattacga
    31081 gggtaagatt acttgatgaa ttgtgggatg cggcaggtga gtttgttaat gacaacacat
    31141 tgactgtgta catcaaacgc ttgcgagaga agatagaaga tgacccttca agtccacaaa
    31201 taattttgac tgttcgtggg acagggtata gattgggggg aagttatgtt tcgaaataga
    31261 gagtttcgaa aatttgctat tttattctta ttaattacta ttctcactgt agccctggga
    31321 tttgcaatca gtattatgac tggaatactt tctattgttt ctgccactac ctttggaata
    31381 gcattttttg tgtttactaa aaatcgatac aaaagtattg cacagatttc agaacaaatt
    31441 aatcttgtgc ttcataatgc taaccatctg tatattgctg aatcagatga gggtgagctt
    31501 tctattctac aaagtgaaat aactaaaatg acgttacgca ttagagagca aaattacgca
    31561 ctgaaaaaag aaaaagaaca ccttgctgac tcactagctg acatagccca ccagcttcgc
    31621 actcctctta catcagtaac tcttattttg tcactgttag agaatacttc tgatgaagat
    31681 gagagaaaag agttgatacg agaaacagag gaattactta tacgaatgga ctggctgatt
    31741 acttcccttt taaaattatc acgtttagat gcaggtattg tagtctttca aaaagaacaa
    31801 attgatgtga ataatttgat tagttctgca cttcatcaac ttttgattcc catggaactg
    31861 cacaatatca ctttacatat tgatatacca aaaggagtaa ggattttggg tgatttaaat
    31921 tggctttcag aagcaattca aaatatattt aaaaactgca tggagagtgt gggtgataat
    31981 ggaaaaattg atatcatttg tgaggacaac tttttgttta ctcaacttac tattcatgac
    32041 aacggagcag gctttaaaaa agaagattta ccatgtctat ttaacagatt ttatcgtggg
    32101 aaaaattcaa gcacagccgg ttatggaata ggattggcac tttgtaagac gattattatg
    32161 agacaaggag gaacaattac tgcacaaaat cacccacaag gtggtgcaat atttgttatt
    32221 cgttttccaa agtgacgaat ctctcattta aatgtcactt aaatgtaagt tgaaagttct
    32281 atgatatatt tataaaatat gaaaggagac ttatataatg gcgtttttaa agattgaaga
    32341 tttatgtaag gtttatggta agaatgaaaa taaggttact gcacttgacc atgtctcact
    32401 tactattgaa aaaggggagt ttactgcaat cattggttct tctggttctg gtaaatctac
    32461 actactacat tccattgcag gagtagacgt accaacaagt ggaaagatat atctggaggg
    32521 gcaagatgta tatggccaaa gcaatgaaaa acttgctatt tttcgtaggc gacaagttgg
    32581 attgatttat cagtttcaca atctgatacc cacattaaat gtagtggaaa atatcacatt
    32641 gcctatactt atggataagc gtaaggtcaa tcaagaacgg ttaaatgact tgttagaatt
    32701 acttggttta aaagaacgaa aaacacattt accaaatcaa ctttctggtg gccaacagca
    32761 acgtgttgcc attggtcgtg cattgatgaa tgcaccagca gttatgcttg ctgatgagcc
    32821 tacaggtagc ttagacagta aaaatggaca agagattatc cagttactga aagagagcca
    32881 tagtaaatat caccaaactc tcattattgt cacacatgat gaaaacattg ctttacaggc
    32941 agatagaatt atctgtattt cagatggaaa agtagtgcgt gatgagagga aggtgaatcg
    33001 ttaatgaaca tttttaacaa agttgctctg caaggattga aaaagaatcg aactcgaact
    33061 cttgtaacaa ttataggagt tgttctgtca gctgctatga ttacagcagt tactaccttt
    33121 ggagtttctt tgatgaacta tatgataaat ggagctatta taaaatatgg tgattggcat
    33181 gtccagtttt tggatgtgga ttcttctttt gtacaggaac aaagagtcaa tgatgaagtt
    33241 gcaaatactg caacatttga aaatattggc tatgcaacac ttgaaggtaa cagaaatcca
    33301 gataaaccat atctttttat tgctggattt agcaaagaag cttttgatac tttgcctata
    33361 actttaattt ctggaagatt acctgaaaat agtagagaga ttcttgtttc aagtaaagtt
    33421 gcgacagatg gtggtgtcaa atttgcagta ggggatacta tttctttgga tgtagggaaa
    33481 cgaatgagtg aaaatgaaaa gcttggtcag agtgacccat acactgctgg gagtgaagtt
    33541 tttgtgccac aggataagaa aacttataca gttgtgggta tctgtagaaa gcccgtattt
    33601 gaagaatctt ctgcaccagg atataccttg ataaccaaag aagataaaca agtcaagact
    33661 gacagtctta gcctttttgt cacattgaaa aatccacatc agattcgctc ttacacaagt
    33721 agcactaaag accatgcttc cattttcaat gacaatgtgt tgcgattcat aggtgtctct
    33781 gataacaatt tgttcaatat gtttttgtac tcggttggtg gcattgtgct tgccataatc
    33841 atgctaggct caatttttct aatatataat tcatttaaca tgtcattgaa cgagcgaaca
    33901 catcaatttg gaatactttc atctgttgga gctacagcaa aacagttacg aaattcggtg
    33961 ctatttgaag gaatttgcat tggtgcgatt ggtataccaa ttggtgttgc aatcggtata
    34021 gtcagtattg gacttgtgat ttctgtcata tctaaaaact ttgcaaatat tctctatgta
    34081 aacgttccgt tgacattaac attgtctatc cctgcaatca tcgttgcagc agtggtaagt
    34141 atggttacaa tcttaatttc tgcttacata ccagcaagaa aggctgccaa cacaccagtg
    34201 atggagtgta ttcgtcagac aaatgaggta aaaattgaat ctaaatctgt gaagacatca
    34261 aaactaacac agcgagttta tggtcttgag ggagttcttg ctatgaaaaa ctttaaaaga
    34321 aacaagaaac gttatcgaag tattgtattg tcgcttgttt taagcatagt tctatttata
    34381 tctacaagtg ctttagtagc agacctaaaa cagatgtcaa atcagaggtc agaggttact
    34441 gaccaggata ttattttttc tacacaaggt atgaaagaca acgaaatgtt gaagctttac
    34501 gataagctaa aaactgcaaa tggtgtttac gaaggtttgt atcagaaact tatgacctat
    34561 tcatgtgttg ccaaagtaag tgagcttaca gacgattatt tgaactatga gggttcacat
    34621 tctatgagtg aaatgcttga attaccagta gatatgcaat ttcttgatga tagaacttat
    34681 ctgaatatta ttaaaagtct tggattacca gcagaagaat atactggaca aaatgcaaaa
    34741 ctgattgctg tttctatggt aacaaaggat aataatatta ttgatatttt caacagttct
    34801 tctatgaatg ttagcctcac tcctgaaact aatggcaagt tggagatacc tgagagacaa
    34861 aatgtgaata tttcatttgt caaagtagtg ccacctgatt ctcctccagg aatcagaagt
    34921 tcagatgtga agaaagcaag cttcagagta ttggctcctt attcagccaa agataagttt
    34981 gagattcctg gtgtttctac aggaattgaa agtttaagct ttcaatcgaa aaatcctact
    35041 cagtcagttg cagaaatgga gcaaattatt gagagtgagg gagttacgaa tgagtacaat
    35101 ctgtacaata tgtatggaat gatggatgag aaccgtaatt acatttttat tgccaatgtt
    35161 tttgcttata ctttcataat tatgatttct ctgatttcga ttgccaatgt gtttaataca
    35221 atttcaacta atattaagtt acgtcgtaga gaacttgcca tgcttcgctc tgtaggaatg
    35281 tctgagcatg atttccaaaa gatgatgaat tttgagtgtg ccttttatgg catgagagca
    35341 ttgttctttg gacttcctat agcagtaatt tcttcatggc taattcataa agtaatggtt
    35401 acagatgata ttgattttgt gattccatgg tttagtattg gaattagtat atttagtgtt
    35461 ttatttatag tatttattac aatgttgtat actattaata agataaaaaa agaaaatatt
    35521 attgatgcac ttcgtgatga catggcttaa ttaaaatgat atggcttaat taaaaatagg
    35581 agggaattag cattagccat atttcctcct attttttgaa aatctcactg ttaggaatca
    35641 ctatctagaa aaatcatatg ttgataatag acaactatat taagatgtgt aagtaaacta
    35701 gattactaca ctagacatct taagtcaatt tctctctttt taacttgtat ttgaatttct
    35761 gttagaagtt cctcttcgtt gtttgtagac agagggtcta ttataggtat ttcatatata
    35821 taattttcaa taggtattct tttatattca acataatcaa ccagtttctt aaaagcggta
    35881 taattttctt tgtaaggccc tttatatgtc atacaaataa aatcaccaga tgaaatttct
    35941 gttatatgtt tttgtgattt tatatcaaat aagtctcttt caacagtaac aaaactattg
    36001 tcataaataa ctttatcttc ttttaaaaat ttatctatag atacaagtcc acttactcca
    36061 ccatagaaca cagatatatt atcttctaat atatttgcta tttgtcttct ggaaagttcc
    36121 atagtttctt cactacttaa tccttcttca tactctatag ataagatact tcttcgtgga
    36181 atatgttttc tcactatttt accaacttca tctactttca tacctttttc catagtttct
    36241 ttctttgtgc ttagtacctt ctttataagc tctaattctt taattttttc atccactata
    36301 ttaattttat ttgttagtaa ttttagaatt tcttcattat tttcatcaag ctttgactta
    36361 atttctttta aaggcatacc taaatatttt aaatatttta taatatctaa ttgtgaaaat
    36421 tggtcaattg agtaatatct gtaattgctt tcttggtcaa catgaacagg tttaaataaa
    36481 tctatttggt catagtacct tagtgtctgt atgcttattt tatgaagttt agacatttct
    36541 cctattgaaa attttttact catataatca cctcatttga gatttaaaat atattccatg
    36601 gatatttgtc aggtggatga catgtaaatt ccattttttc ttttgtagta ggatggcaaa
    36661 tttctatctt attggaccaa agagatattt gttgtccaat cttatttatt tctttgccat
    36721 atctttggtc tccgtataat ggatgttttc tgcttgcaaa ttgaactctt atttgatgag
    36781 aacgaccagt ctttaaatta acttgaacta aacttagatt ttctttaaaa tctaatacat
    36841 tatagttaag ttcagcttct ttggcatctt tatggctttt tttcactaca gttaccatat
    36901 tagttttttt atttttatat aaaaaatctt ttagtatatc tgagttagat tttatcttgc
    36961 catgaacaac agctagataa gtttttttga aagtcttact tcttacctgc tcagacaatc
    37021 tagatgctgc ttttgatgtt tttgcaaaca ccataactcc acctactggt ctatccaatc
    37081 tatgtattaa accaatatat acatttccag gtttgttata ctttttttta atatagtctt
    37141 ttaaaagatt taccatatct ttatcattag tattatctcc ttgagacaat atgttaactg
    37201 gtttttctac aactaataaa tggttatctt catatatgac ttttatcatt attttttctc
    37261 ccatcttcca aatataccac aaggaagtat cttaccatct ctggaagctg gtattcctaa
    37321 ttccccaccg tatatatctc ctgtatttgt ctttttacct attgttgtag acaatacatt
    37381 ttcaagaact attggagaaa atcctgttgt ataagaatta attaagaaaa ataaagggtt
    37441 atctgataaa actcccatac ataaatctac aaaactgtat aacttttctt ctatttgcca
    37501 tacttctcct ttaggacctc ttccataaga aggagggtcc attattattg catcatattt
    37561 tttacctctt cttatttctc tttcaacaaa ctttacaaca tcatccacta taaatcttac
    37621 tgtttgattt ttaagacctg ataattctat attttctttt gcccaattaa ccataccttt
    37681 tgcagcatca acatgacata cttctgcacc agcagatgca catgctacag tagctccacc
    37741 tgtatatgca aaaagattca acacttttat aggtctgtta gctttcttta ttttctctat
    37801 catccaatcc cagttagctg cttgctctgg gaataatcca gtatgtttaa agccagttgg
    37861 tttaatgtga aactttaagt ttttataatt tatagtccat tgttctggat atcttttttt
    37921 gtgttcccat tgacctccac ctttattgct tctgtgataa tgtccatgag ggtttttcca
    37981 taaagcccat tcagattcca ttggccatac aacctgtggg tctggtcttc taagtacata
    38041 acttccccaa cgttctaatt tttctccatt tcccatatct ataagttcat aatctttcca
    38101 tttatctgct aataataaca tatttttcct cctataaaat aactctcaat cctatgtatt
    38161 ataccacaaa attagtcatt ttttgctgtt ttagatactt tctattcttt acatttacca
    38221 ttaacattat tactgtttta agcatattat attgtatctg atttttttat aaaaggggga
    38281 ggattttaat ggaaaattat caatctac
//
LOCUS       DAELTA010000014        38040 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.014, whole genome shotgun sequence.
ACCESSION   DAELTA010000014 DAELTA010000000
VERSION     DAELTA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.014"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            252..1436
                     /locus_tag="KPG13_000655"
     CDS             252..1436
                     /locus_tag="KPG13_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984399.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1813..2922)
                     /locus_tag="KPG13_000656"
     CDS             complement(1813..2922)
                     /locus_tag="KPG13_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0984400.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3385..4578
                     /gene="nifV"
                     /locus_tag="KPG13_000657"
     CDS             3385..4578
                     /gene="nifV"
                     /locus_tag="KPG13_000657"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG0984401.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4603..6528
                     /locus_tag="KPG13_000658"
     CDS             4603..6528
                     /locus_tag="KPG13_000658"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG0984402.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6550..7545
                     /locus_tag="KPG13_000659"
     CDS             6550..7545
                     /locus_tag="KPG13_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG0984403.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7870..8343
                     /locus_tag="KPG13_000660"
     CDS             7870..8343
                     /locus_tag="KPG13_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0984404.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8377..8871
                     /locus_tag="KPG13_000661"
     CDS             8377..8871
                     /locus_tag="KPG13_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0984405.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8968..9489
                     /locus_tag="KPG13_000662"
     CDS             8968..9489
                     /locus_tag="KPG13_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0984406.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            9678..9989
                     /locus_tag="KPG13_000663"
     CDS             9678..9989
                     /locus_tag="KPG13_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0984407.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10017..10703
                     /locus_tag="KPG13_000664"
     CDS             10017..10703
                     /locus_tag="KPG13_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0984408.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            10935..11819
                     /locus_tag="KPG13_000665"
     CDS             10935..11819
                     /locus_tag="KPG13_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0984409.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            11918..13093
                     /locus_tag="KPG13_000666"
     CDS             11918..13093
                     /locus_tag="KPG13_000666"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG0984410.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            13221..14075
                     /locus_tag="KPG13_000667"
     CDS             13221..14075
                     /locus_tag="KPG13_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984411.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            14119..15354
                     /locus_tag="KPG13_000668"
     CDS             14119..15354
                     /locus_tag="KPG13_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG0984412.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            15455..16396
                     /locus_tag="KPG13_000669"
     CDS             15455..16396
                     /locus_tag="KPG13_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG0984413.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(16615..16968)
                     /locus_tag="KPG13_000670"
     CDS             complement(16615..16968)
                     /locus_tag="KPG13_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG0984414.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            17264..18175
                     /locus_tag="KPG13_000671"
     CDS             17264..18175
                     /locus_tag="KPG13_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG0984415.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            18435..18869
                     /locus_tag="KPG13_000672"
     CDS             18435..18869
                     /locus_tag="KPG13_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984416.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            18892..20427
                     /locus_tag="KPG13_000673"
     CDS             18892..20427
                     /locus_tag="KPG13_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984417.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            20511..21878
                     /locus_tag="KPG13_000674"
     CDS             20511..21878
                     /locus_tag="KPG13_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0984418.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            21913..22137
                     /locus_tag="KPG13_000675"
     CDS             21913..22137
                     /locus_tag="KPG13_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG0984419.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(22587..22985)
                     /locus_tag="KPG13_000676"
     CDS             complement(22587..22985)
                     /locus_tag="KPG13_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG0984420.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(22988..23389)
                     /locus_tag="KPG13_000677"
     CDS             complement(22988..23389)
                     /locus_tag="KPG13_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0984421.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            23749..24735
                     /locus_tag="KPG13_000678"
     CDS             23749..24735
                     /locus_tag="KPG13_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984422.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            24719..25630
                     /locus_tag="KPG13_000679"
     CDS             24719..25630
                     /locus_tag="KPG13_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984423.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            25743..27311
                     /locus_tag="KPG13_000680"
     CDS             25743..27311
                     /locus_tag="KPG13_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0984424.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            27386..28411
                     /locus_tag="KPG13_000681"
     CDS             27386..28411
                     /locus_tag="KPG13_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984425.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRVLPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            28404..29348
                     /locus_tag="KPG13_000682"
     CDS             28404..29348
                     /locus_tag="KPG13_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984426.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            29707..32364
                     /locus_tag="KPG13_000683"
     CDS             29707..32364
                     /locus_tag="KPG13_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984427.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            32579..33208
                     /locus_tag="KPG13_000684"
     CDS             32579..33208
                     /locus_tag="KPG13_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984428.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            33343..33669
                     /locus_tag="KPG13_000685"
     CDS             33343..33669
                     /locus_tag="KPG13_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984429.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            33685..34974
                     /locus_tag="KPG13_000686"
     CDS             33685..34974
                     /locus_tag="KPG13_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0984430.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            34998..35327
                     /locus_tag="KPG13_000687"
     CDS             34998..35327
                     /locus_tag="KPG13_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0984431.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            35475..36803
                     /locus_tag="KPG13_000688"
     CDS             35475..36803
                     /locus_tag="KPG13_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0984432.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            37120..37980
                     /locus_tag="KPG13_000689"
     CDS             37120..37980
                     /locus_tag="KPG13_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG0984433.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEIEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
BASE COUNT        15032 a         3964 c         6811 g        12233 t
ORIGIN      
        1 aataaagaaa aaatgtataa tatttaacat taaaaaagat atttcataaa attaaaaaac
       61 aaagtgaatt tgaatactat ttaaaaaata aaataaatat ataaaaaata tttaatataa
      121 aggtggaaat aagttgaaaa tgtaaagtca ttggaaaaat atagttgaat ttaatgagca
      181 aaaatgttat tgttttatca ataaagtgtt aaaataaaaa ctttcaatat cattttaggg
      241 aggatgattt catgcaaaaa agtgtaaaga aagcaaaagt aacgggctct atgttagcta
      301 tttttggtgt tgcaagcgtt ctttttagtt cacatgcagg aggaggattt gctacaggga
      361 atcaagaaac tcagtactat gtgcagtatg gatggacagc accacttatg gcaattttag
      421 caatgattat acttactgct accatgagag aagttatcat aatgtataat aataacaatt
      481 gtaggaatta taaggattta ttttgtgagc tttggagacc atatccaaaa cttgaaataa
      541 tatgggagat atattactat ctaatggttt taatagctgt aagtgctgtt atagcaggtg
      601 cagcagcagt atttcaaagt ataggagtaa attattttgt agctgtattt attattggag
      661 ttgtacttct tgtctttact atatttggtg ctatgcttgt atctaaagca gctacagcaa
      721 tgacaatagc tattctagta tgtactctta ctatttttat tgttggtata aaagctaaag
      781 taccagaaat aactgaaata ttatcaaata gaacgagttt cacacctggc tatatcaagc
      841 caatacttaa tacttttatt tatgcaggtt ttcaatcggt tgttatacct acacttgcag
      901 gatgttcaag accacttctt aagaactcaa aagaagctac taaggcaatg ataatttcat
      961 ttgttatgaa tgcaattgca ttaggacttg ctgtaactat gctaatggga tggtatagag
     1021 aaattattgc tgctgggcaa acaacactcc ctactcttta tgttgcaggt caatctggaa
     1081 atcatactat atacataatt tataatgttg cattattcct ttgtttaatg tctacaggag
     1141 taactactat atttggtctt gtaaatagat ttgaagacca taaagcactt tctttccttt
     1201 cttcaagaat gaaaagaagg gtatttacag cttgtgcaat aatggtagtg tcaatgctta
     1261 tatcacttac aggattaagt aatattgtta aatatggata tggatattgt ggttatcttg
     1321 gtctattcac tatagtaatt ccatttctta cactaggaca ttataagaat aagaagtttg
     1381 caaaggaaaa tccagaagct aaatggccag cggaattaaa tgaaaatgta aattagatgt
     1441 agtagcaaaa aatattgcta aagagaatac tataaataag aatactatag ataatatatc
     1501 tgcatgttta ttaagattat ttgcaaaatc taatattaaa caactataaa caagcaatat
     1561 aaaactaaga ataaaagata aaaagagggt atctcaaaat ggacttttag tttataaggc
     1621 actctttttt atatgaagtc aaatatattt tcattatttt gacaataaaa gagactaatc
     1681 ctactttgga tagcctcttt tttctttaat ataaaatagt ataacagcaa agtatagatt
     1741 gatacatgtt ttctgtagct ttctatatat tacagtgttt gtgtggcatt ggtattttaa
     1801 gcaatctata aattataatt ctataattct tctactaatt ttagattcta tagccgtctc
     1861 tatttcactt aacattcgtt catttttagg gtcaataaaa gcacatgtat agccttcttc
     1921 accagctcta ccagttctac caatacggtg gatgtagtct tctggtgttt ctggtaaatc
     1981 gtaattatat atatgactta ctccagatat atcaagacca cgagaagcta catctgtagc
     2041 tattaagtat tgtatatcta aatttctaaa tgatttcatt attctttctc gttttgattg
     2101 agcaatatca ctatgaagtt tttgacaatt atatcctctt atagcaagag cttcttcaag
     2161 attgtcaacc cttctcttag ttctacaaaa tattattgcc ataaaaggat tatcttcatc
     2221 tagtacctta cataaggcat ctaatttttt tctatctgta gtttctacta cttcttgttt
     2281 tatattatta agagtgattt cttctttttg aatagaaact acaacaggat tttttgtgta
     2341 tctataagct aattttttta cttgagaatt tatagttgca gaaaagcata gtgtttgctt
     2401 tttgtttgaa gtctctttta aaatagcttc cacttcattt ttaaatccca taagcaacat
     2461 ttggtctgct tcatctaaaa caaaagtctt taacttacta aggtctattg tttttctatt
     2521 tagatggtct aaaagcctac ctggtgtagc tataatcata tgaatatttc cttttagttt
     2581 ctttatttga gaaccaatat cttttcctcc atatgctgca agaatactta catctttaga
     2641 ttctttaagt ttcatagctt cttctgttat ttgaatagct agttctcttg taggagaaag
     2701 tataagtact tgtatatcat ttatatctaa tgatatattt tcaaatatcg gaagtaaaaa
     2761 agctagtgtt tttcctgttc cagtttgagc ttctgctatt acatcttttc cttcttttat
     2821 tagtttaata ctttcttttt gtataggtgt aggtgttgtt attcctgatt tttttagaat
     2881 atttattgta ttttcactaa tccctaagct tttgaaatcc attgttatct tacctttcat
     2941 gaaaaatttt cttttttatt aaccaattaa taatatcata tttgtaagta tttttagtaa
     3001 attttttaat tattatttat atagcttaaa aatatattaa gatactttaa aatggaatgt
     3061 aacttaaatt aaattttagt aggaatttta taggtttatt tttaaaggtt ttttgaaaat
     3121 aaatctattt aaaaattaaa ttaaaatgca agaaaaacat aataactttc atttttaaga
     3181 agttttcaca aaaaagaaaa aatatagtgc aataaatgat acaaagtaat ataatagatt
     3241 acaagatatg aaaagctaaa aaaataaatt gcaaatgaaa ttaaaatgaa taaaaaataa
     3301 aaaaataatc ataagttaga agtatcaatg aaaaataaga cttaatgttt ttaaaattaa
     3361 aagtatacta aggggggcat taatatgtgc gttatatcaa aagatagagc aaaggaaata
     3421 aaaatagtag atactacttt aagagatgga gagcaaacag ctggggttgt atttgcaaat
     3481 agagaaaaaa taatgattgc tgaaatgtta agtgacctag gtgttgacca aattgaagtt
     3541 ggtataccta ctatgggtgg agatgaaaaa aatgtaataa agcacatatg tagtagaaat
     3601 ttaaaatcag atataatggc ttggaataga gcagtaataa aagatgttga agaatctatt
     3661 tcttgtggtg ttgatgcagt agctatatca atatcagtat ctgacattca tatagaaaat
     3721 aaattgagaa cttctagagg ctgggtttta gaaaatatgg ctaaaacagt tgaatttgca
     3781 aagaaaaatg gattatatgt ttcagtaaat ggagaagatg cttcaagagc agatatagac
     3841 ttcttaactg aatttataaa tgttggaaaa caagcagggg cagatagatt tagatactgt
     3901 gatacagtag gagttatgaa tcctttttct ataaagaatg caatagaaac tctttatgaa
     3961 agaactaatt ttgatataga aatgcataca cataatgact ttgggatggc aactgcaaat
     4021 gcacttgcag gaatagcagc aggagcaaat tatgttggtg ttacagtaaa tggattaggt
     4081 gaaagagcag gaaatgcagc attagaagaa gttttaatgg cacttaagtg tgtttataaa
     4141 tgtgatttaa ataatataga tactagaaaa tttagaggaa tatgtgagta tgtagcacag
     4201 gcatcaggaa gaatacttcc tacatggaaa ccagtagttg gggataatat gtttatacat
     4261 gaatctggta tacatgcaga tggagcttta aaagacccac ataactatga accatttgac
     4321 ccaagtgaag taaatcttga aagaaaaata gtaataggta aacattcagg aagagctgca
     4381 gttgtaaata agttgagtga atatgaaatg tatatttctc cagaaaacgc taccaagcta
     4441 ttaaatgcta taagagctac atctataaga ttaaaaagaa gtttaatgga taaagaaatt
     4501 cttcaattat attgtgatat actagcacat gagaaaggca caacagaaga agaagcagtt
     4561 agaggttcat atatttaaga tataattgtt taggaggaaa atatgggaga taatatagtt
     4621 tataaaataa ttaaaaaaca tatagtagat ggagaagctg ttgctggaag ttcaattggt
     4681 ataaaaatag accaaacttt aacacaagat tctactggaa caatgacata tcttcaacta
     4741 gaagctatgg gaatagataa agtaaagact aaaagaagtg tggcatttgt agaccataat
     4801 atgttacaac aaggatttga aaatgcagac gaccataagt atatacaaac agtggcagat
     4861 aaatatggag tttatttctc taagccagga aatggtattt gtcatcaagt atttttagaa
     4921 agattttcaa caccaggaga tacactttta ggttctgata gccacacacc tactgctgga
     4981 ggtgtgggca tgatggctat tggagctggt ggattagatg ttgctttagc catggcagga
     5041 ggagcatact atataaaagc accaaaagtc tgtaaggtga atctagttgg aaaattaaat
     5101 aatatggtat catcaaaaga tataatatta gaagtgttaa gaaaacaaac tgtaaaaggt
     5161 ggagtaggta aagtttatga atatggtgga gaaggtgtca aaagcctatc tgttccacaa
     5221 agagctacaa taacaaatat gggggctgaa cttggagcaa ctacttcaat cttcccaagt
     5281 gatgaaaaaa cactagaatt ttttaagagt caaggaagag aagacgcatg ggtagagcta
     5341 aaaccagatg cagatgcagt ttatgatgaa gagattacta taaacttgga tgaattaaaa
     5401 ccattagctg caaaacctca tagcccagac aatgtagatg aagttgaaaa tataggcaag
     5461 attaaaatag accaagtggc aataggaagt tgtacaaatt cttcttatga agacttaatg
     5521 aaagtagcac aaatcctgaa aggaaataaa gtacataagg atgtgagttt ggtaatagca
     5581 ccaggttcaa gacaagttat ggagatgatt gctagaaatg gggctttggc agatataata
     5641 agtgctggag ctagaatact agaaaattct tgtggtccat gtattggtat gggtcaatct
     5701 ccaggtactg actctgtgtc tttaagaact ttcaatagaa atttctatgg tagaagtggg
     5761 actttgtcag cacaagtata tttagtaagt ccagaagtgg cagcagtaag tgcaatcaag
     5821 ggagtgctta cagacccaag agaatttgat attaaattta caaatttaga tgtaaatgag
     5881 tttttgatag atgatagcat gataattaaa cctgctgatg taggaagtga tgtagaagta
     5941 gttagaggac ctaatataaa acctttccct cttaatacag agctaagtca gtctataggt
     6001 ggaaaagtta tattaaagac agaagataat ataactactg accatataat gccatcaaat
     6061 gctaagttac ttccttttag aagtaatata ccgtatcttg caaactattg tttcaatact
     6121 gtagatacag aatttcctca gagagcaaaa gacaataatg gtggatttat agttggtgga
     6181 gataattatg gtcaaggttc aagtagagaa catgcagctt tagcaccatt atatctaggt
     6241 gttaaagggg taatagttaa gagctttgca agaatccaca aggctaactt gataaatagt
     6301 ggtataattc caatggagtt ttgtgatgag aaagattatg aaaatatatc tcttttagat
     6361 aatctggaga taccaaatat tttagacaat ctaggtagtg gaattttaga agttaagaat
     6421 acaactaaag gaacttcttt taaggtcaaa gtagaattgt cagcaaaaga agttgatgta
     6481 ttaaaagctg gtggaaaatt aaactacact aaaaatcaag ctaattaata tgaaaagagg
     6541 gaatattgaa tgtataaagt gacacttata ccaggagatg gaataggacc agaagttgcg
     6601 aaagctatga aaaaggtagt agaagctact ggagtagaaa tagaatggga agaagttaat
     6661 gcaggagagg cagttattga agagtatggt actccacttc ctgaatatat tatagatagt
     6721 ataaaaaaga acaaaatagc tataaaaggc ccaattacta ctcctgtagg taaaggattt
     6781 aggagtgtaa atgtaacttt aagacaggca ttagatttgt atgtcaactt aagacctatt
     6841 aaaagtttta aggggataaa atcaagatat gaagatgttg atttagtggt tgtaagagaa
     6901 aatacagaag atttatatgc tggaatagaa cataaaatag gtgattatgc agctgaaagt
     6961 ataaagataa taacaagaag tgcaagtgaa agaatcgttg attttgcatg taactatgtt
     7021 aaagataata agagaaaaaa agttacagct atacataagg caaatataat gaaaatgtct
     7081 gatggattgt ttttagatgt gtttagagaa gttgcatcaa aacatggtgt agaatatgat
     7141 gatttgattg tagatgcagc agctatgaac ttagttttaa atccagaaaa ttatgatgta
     7201 atggtaatgc caaatcttta tggagatata ctatcagatt taggtgctgg gcttgttgga
     7261 ggtttaggta taattccaag tgcaaatatt ggtaaagatt gtgcgatatt tgaagctgtt
     7321 catggaagtg ctccacaaat agcaggtcaa aataaggcta atccaacagc acttattcaa
     7381 tcatcagtaa tgatgcttag atacttaggt gaatatgaaa atgcacaaaa aatagaaaca
     7441 gctttagaaa aagtatttct tgaaggaagt aaattgactg tagatttagg tggctcagct
     7501 tctactacag agtttgcgga tgaggtgtgt aagtatattg tatagatttt atttagggaa
     7561 aaagtaaaat atagattgaa gaaaagaggc tttctcaaaa tagagataag ccttttttat
     7621 tggaatgata aaatatattt gattttatac aaaagagagt tcctcataaa cgaaaaagtt
     7681 aattttggga tatccatttt aattttaaaa ttaattgatt aatacgcaaa gaaagattaa
     7741 ataaatttat tatttttaat tgaactttca accaaaatat ggtaaaattt aaaaagttga
     7801 actttcaact aaaaatgaaa atattgatta tgtgaaattc tataaaacag aaactatttg
     7861 actttagcca tggtagtttc agtggaggtg tgtttgatta agactttaga tagtaatata
     7921 ttaagagaag taggtacttt atctagagca gtaaattcaa taaatgatat taagtacaag
     7981 gaattaaagt tacaaaaagg acaatttaca tttttgacaa gaatctgtga aaatcctggt
     8041 ataaatcttg tggaattatc aaatatgtta aaggtagaca aggctacaac aaccaaggct
     8101 atacaaaaat taataaaggc aggatatgta gataaaaagc aggataagtt tgataaaaga
     8161 ggatataatt taactccaac tgataaatct ttggaagttt atgagttgat aattgaagaa
     8221 gagaatagaa gtatagagat atgttttgat aattttacag atgaagaaaa acaagtggta
     8281 actaagttat tagaaaaaat gagcaaaaac gtagaaaatg aatggtttaa agtaaagaga
     8341 tagtaaataa atacattaac ttaaggagat atttttatga ttagaaaagc gaatatgaat
     8401 gatttagaaa gcattatgaa aattataaaa tctacagtgg aagaaatgaa gacttataat
     8461 aatacacaat gggatgaaaa ctatccatta gaaaaagact ttgtttcaga tattaagaaa
     8521 caagatttat atatttatga agtggatgga gaagtagcag ggtttatatg tcttaattat
     8581 gaagagccag aagaatattt aggcttaaat tggtcatcta ataaaaaagc aatggtaata
     8641 catagaatgg ctgtaaatcc aaactttaga aagacaggaa tagcatctaa gttagttgat
     8701 tttgctgaaa aattagctgt agaaaacaat gtatcatact taaagagcga tacatattca
     8761 ataaactcaa aaatgaattt attacttaca aagtgtggtt ttataaaaat cggagaaatg
     8821 agttttttag gaaaggagaa atctttctac tgttatgata aaatattata aagagcattt
     8881 ataatacaag gggtaaataa tagattttca gcattagtaa gggcataata taacctagta
     8941 tatgctatta tgaaagggat gatttatatg attggatttc taaagaaaag aagaagtatt
     9001 agaaaataca aggatgttga agtagaaaaa gaaaaattag ataaaatatt aaaagctgct
     9061 cttttagctc catcatcaaa ggggcttaga acatgggaat ttatagtggt agatgataaa
     9121 gaaaagctaa ttaatttatc tcaatgtaga actaaaggtg gtggattttt cttaaaaaat
     9181 gcacctttag ctattgtaat tattgcagat aaagaaaaaa atgatgtttg gatagaggat
     9241 gcaagtatag ctgcttcata catacaactt caagctcatg aactaggttt aggttcttgt
     9301 tggatacaag taagaaatag aatgcatgat gataatatag aagctgataa gtacattaga
     9361 gaagaattaa aagtacctag caagtacagt gtagaatgta taatttctat aggatatcct
     9421 gatgaagaaa agaaagcata cggtgactct gatttagact ataaaaaggt tcattttaac
     9481 aacttttagt atataatatg ttgtattaag acattatatt tttttctatt gttaatatat
     9541 acttaaaatt gtaaaaatat atctgaattt tactaactta tctaggggtt atgtacaagg
     9601 attttaaaga taaatataga aattaatgtt tataatcgat acataacttg agtaacaaaa
     9661 tttaaggagg ttaaagtatg ggtatattta aaacaaaaat ggatgaggac tggaaagtaa
     9721 actacattaa agagtttaat gaaatgagag attcttatga gagtaaactt caaaaaaaac
     9781 agtttgaggt agatagtttg aaatcagaat tggatagact aagaagctat aaaaatagcc
     9841 ttaagccaaa agaaaaacaa attactgatg atgatataaa taatataaag agtttaagaa
     9901 gagatggctt aagctataaa gaaatttcaa atcaaacaag ttggagcaaa gcaactgtaa
     9961 gtagggtatt aaatggactt tacgattagc atatacatgg ggtgatttaa attgggatga
    10021 agtatagtta caaaaaatgc ataatagatt ctattttatt gatgtttata gttcaaatat
    10081 taaggatgat tttgaattat gtacttctaa gtcaatttga atttactctt gaaaatttta
    10141 atattataaa cttaatttct tttacattag ttggtttatc gttaatttta tttttaaaag
    10201 ataattcttt gtataacaag gttagaaata gaaaaatcac agaagctttt gaagaaaata
    10261 aaaacaatat acttattgaa aagtgtaagc tgatactttt tgttgtggtt ttatctttag
    10321 caataattgt aacttattgt actaagggat atgtactttt taatgtaact atgatgactt
    10381 tgtctgtatt gatagtccca atatttgaag agttattttt tagagaatat atatggaact
    10441 atctaagtaa ttttataaag tcaaagagta aaattatctg tataacatct atactatctg
    10501 gaatttataa tataggatac atagatgtta taagaaatta tgtaatattg tataacaatt
    10561 caagctacac ctttgaagtt attatttcaa aaattatgat aggtactgtt tttggtattg
    10621 tacttggatt agttaagtat aggtttagag atgtagggtt ttgtatatta ttgaggagct
    10681 tatttgctat atttattagg tagatagtat aatcggttta taaggttgtt ttaaatttaa
    10741 ttttaaatca accttatttt atttgtgttt tataaagcac ttatatatta ataactaata
    10801 aatttaataa aaattatttg aatattttta aaatatataa atatgtattg acatataaat
    10861 attatgaatt acaatttata taaactaata aaaattaaat aatattaatc aacttattga
    10921 atggaggaat aaaaatgaaa tttgtgactt tttgtagtag caatgaagaa aaaataggtg
    10981 tatttaattc agaaacaaat agtatatatg aaattaatag tttaggatta agtaaattat
    11041 atactgatat gaatgatttt attgaaaacg tatctacagg agatttagaa aaaataaaaa
    11101 acaattcatt tgaaaacgca aagtgttata agttagaaga agttaaatta tgttccccaa
    11161 ttgtaagacc taaaaaagat attatatgtt taggtctaaa ctataaagac catgtcaatg
    11221 aaatcccaga tggagttata aaaaacgtag ttatgccaga ttatccaatt tatttttcta
    11281 agagagcaga caaaataatt ggagtggatg ataagataag cttacatgga gatttggtag
    11341 agaagttgga ctatgaatca gaacttgcag taatcatagg taaagaaggt ataaatatat
    11401 caaaagaaga tgcttatgaa tatatatttg gatatacaat agtaaatgat ataagtgaaa
    11461 gagcacttca agataaacat gttcaatggt ttagaggaaa aagcttagac acacatacat
    11521 ctatgggacc ttgtatagta cataaagaag aatttgaaca tcctttaaaa ttagatataa
    11581 gtagtgtagt aaatggagaa gtaagacaag attctaatac agagtacttt atatttgata
    11641 tacctactat aataaatgat ttatcaagag gaatgactct aaagccagga gatataattt
    11701 caactggaac tccagcaggg gttgctatgg ggatgaatcc tcaagtatat ttaaagcatg
    11761 gcgatatagt tgaatgtaaa gtcgaaggaa taggagtttt aaaaaatata gttgattaat
    11821 ggataaatat gccatataat tttattagta cgaataattt aattaatata ggattaatca
    11881 aacttatgat taatttatta tagagaggga tgagtatatg gcaagagaac tagaattaaa
    11941 gtatggctgt aatccaaatc aaaaaccatc aaagatttat atgaagaatg gagaacttcc
    12001 tatagaggta ttaaatggaa aacctggata cataaatttt ttagatgcat ttaatagttg
    12061 gcaacttgtg aaggaattaa aagaagctac aggtcttcca gcagcaactt catttaaaca
    12121 tgtaagtcca gcaggagcag cagtaggggt acctttatca gatactttaa agcaaatata
    12181 ttttgttgat gatttagagc tttctccatt agcttgtgct tatgcaatgg caagaggagc
    12241 agatagaatg tcttcttatg gcgattttat agcactttca gatgtatgtg ataaggagac
    12301 tgcaactatt atagcaagag aggtttctga tggtataata gctccaggat atacagatga
    12361 agcccttgaa atattaaaag gaaagcgtaa aggtaactat aatattgtaa aaatagatga
    12421 aaattatact cctgaaccta ttgaaacaaa ggatgtttat ggaattactt ttgaacaagg
    12481 tagaaatgaa atattaataa atgaagacct attaaaagat attccaactg ataataaaat
    12541 ttttacagat agtgcaaaac gtgatttaat tatagctctt attacattaa aatatacaca
    12601 atcaaattct gtttgttatg ctaaagatgg acaagtaata ggggtaggtg caggtcaaca
    12661 atcacgtatt cattgtacta gacttgcagg taacaaagct gatacttggt atcttcgtca
    12721 acatcctaaa gtattaaact taaaatttaa aaaggatata ggtcgtccag atagagataa
    12781 cactattgat gtatatcttt ctgatgatta tatggatgtg cttgctgatg gcatatggca
    12841 aaactttttt gaagaaaaac cagagcctct aactggagaa ggaaaaagag catggcttaa
    12901 aactttaaca ggagtagcac ttggttcaga tgcatttttc ccatttggag ataatataga
    12961 acgtgcaaaa agaagtgggg taagttttat agcacaacca ggtggttcta ttcgtgatga
    13021 taatgttata ttaacttgta ataagtataa catagtcatg gcatttacta aaaatagatt
    13081 attccatcat taaaatactg aaagtataaa gataatatag attaagtaaa aatctacaac
    13141 aagttttgtt gtagattttt ttatttgctg tataatttta aatagaactt tatttttaga
    13201 tattcaattt ggaggttgat atggctttaa atataagaaa ctatagccta ttatgtaatc
    13261 aaggaacagc taaatttaat gaagatgtag tcggtataaa tccatttggg gcatgggttt
    13321 tagatggtgc tacaggactt aatggaaaaa atttagtgtc aactgaaagt gacgcaaggt
    13381 ggtatgttca gtggtggaat aggtatttat ataaaaatat aaataaagaa gaatcattaa
    13441 agagtattat taatactgga ataatagaag taaaaaaaga gtatgaacta agattaaatg
    13501 ggtttaaggt tgaaaaatta gatttaccat ctagttctat agttgttgta aaatttcatg
    13561 aaaataaagt agagtatttt ttattaggag attgtgcttt gtttattaaa aatggagatt
    13621 ccaaagttat aaaagatagg agtatttgta aatttgataa tactgtattt gatgaaatga
    13681 gtaaactttc taacttggaa gagatggctt ttgatgagat tagaactagt gttatggata
    13741 caatagtaag caatagattg aagaaaaata caaaagaggg ctattggata ttggattttg
    13801 atgaggaagc tgtggaaaat tctatatatg gatacattga aataaaaaaa gattttcaat
    13861 tgatgctaac gagtgatggt tttacaagtg catgtgatag atataatcta ataaaagaag
    13921 aagaattgat acgtatagca gaaaaatttg gtataggata tatacataat aaagtaaggg
    13981 attttgaaga taatgactat aaagcggtta aagtgccaag atttaaagta aaagatgatt
    14041 catcttgttt atatttagat atatataatg attagactgt ataaaactat ttaattttag
    14101 aaaggaggca agtgaaaagt gaaaattctt catataataa cacaaaaacc aaatagtaca
    14161 ggtagtggta tatatttgtc tggtatgata aaagggttcg aaaaaatcgg tcataagcaa
    14221 gctgtaattg caggtataga tgtaaatgat gatgtaaatt gttttccaag tgaagtaagt
    14281 ttttatccag taaagtataa ttgtggggag ttaaattttc ctgttgtagg tatgagtgat
    14341 tctatgccat atgaaagtac aagatataaa gatttaaata tagacatgat aaatagattg
    14401 aaatatcaat ttaaagtaaa tatagataag gctatgaatg attttaaacc agaacttata
    14461 atatgtcatc atttgtacct cttaactgca tttgttaggg aaatggtaaa agatataaag
    14521 gttatgtcta tttgtcatgg aacttgttta agacagttaa ataccattga tttagagaaa
    14581 gaatacataa tagcaaatat aaggaaatta gatttaatat ttgctcttca tgaaaatcag
    14641 aaatatgata ttataaagac ttttggagta agtgaaagta aagtagttgt aattggtagt
    14701 ggttataatg atgatatatt ttataataaa aattataaaa taaaggatga taaaataaaa
    14761 atagtatttg caggtaagat atgtaaatca aagggactga ttccatttat aaaagctata
    14821 tctaaattaa aatatagtaa agatttaata gaagtcaatt ttgcaggtac tggaagcgat
    14881 atcgaatcat ataatgagat agttaaactg gcaagtaagt ctccatttaa aatgaatttc
    14941 ctaggaaagt tagaacagag agatttagct gagctattca acaggtcaca aatatttgtg
    15001 ttaccatcat tttatgaggg attaccagtt gtagttttag aagctttatc ttgtggcaca
    15061 gatgtaatca ctactgatat tcttggtgtt aaagagtgga ttggtagtga gattaataat
    15121 tctggaaaga tagaatatgt ttctctacct tttatggaga aagaaggtat tccaaaagat
    15181 gaagagttat atgattttga aaataatttg tataatgcaa tagattctaa gatacaatct
    15241 ttattgaata actcaaataa gaaaacatct gtagatatgt caaaaaaaac gtgggatggc
    15301 ttagcatata gaataaatga agttatttta atggatgaat tgtgtttagt ctaaaattat
    15361 ttaaattata aaaaagtgta actattttta cactattttt aaatgatttt atattatact
    15421 aatactaaat atatttgttt tagggaggta atttatgagg attagtaaaa aggtattggc
    15481 gttaggtatt tcagcattac tgttaagtgt tagttttcca acatcaataa acgcactaga
    15541 taagattgaa aatattcaag gagtagataa atatgaaaca gctgggctta tttcagataa
    15601 gcaagatttt acaacagcag ttcttatcaa tgcagactct acaatggtag atgggattgc
    15661 tgcaagtggt cttgcaggag taaataatgc agctatttta ttaactaata aggatgatat
    15721 accagaagca acacttcaaa gactgaatag agttactaag atatatgtaa taggtggaga
    15781 aaattctata agtaaagatg tagaaaaaat gttacttatg agaagaatgc aagttataag
    15841 aattgatggt gtagatagag tagatacaag ttataaaatt gcaggtgaaa tagaaaaaat
    15901 taaaaacagt gataagatgt ttttagtaaa tggatttaag gatgaagctg atgcagtaag
    15961 tgttgcatct gttgcttata gagatggagc tcctataata ttgacaaaag atataccttc
    16021 agcagaagaa gacacagact tagacccatg gtttggagtt tctcctgttc cagtatatgc
    16081 tattggagga gagtctacat taagtgatta catagtgagc agatatagag caactagaat
    16141 tggtggtgtt gatagatatc aaactaataa aaatgttatt gaaaagttct ataatggagc
    16201 aaaagagttc tatataacaa gtggagatga tttagtttat gctttagttg catcaccact
    16261 tgcaaaaaat gctcctgttg ttttagtttc aaataaaagt gataagagta tattaagtgg
    16321 agctagtaaa gtaacagcaa taggtataag tgataagagt attatagagc aatgtttgga
    16381 tgctgtaaag aagtaatgga gtgttattag taaaatgaat atatacattg aaaattagta
    16441 gattgttcat tttataatgg tataaaatat atgggaagag gctatcaaat ggagataagc
    16501 ctctttttca ttgttaaaat tatgaaaaga tactttattt tatataaaaa agtatcttat
    16561 gaactaaatg tctattttga gacactatct acttttctta tattatatta ttttttatat
    16621 ggaataaatt tcataagtat tatcttcctt taatttttct aaccaaacca tctctaaaat
    16681 atctagttct tctaaatagt ctttctctgt tttgttatca tcatgtttta taagttctaa
    16741 aacttcaaat gtaaatgctt tttcaccata ttttttccat tcattattta attcttttat
    16801 catacatgag ccagcaccaa gctgaaattt gagagagtta aatttgcttt ttatgtcatt
    16861 actttttcca aggtaaatct ttttagtaat atcatttttt attatgaata cacctgtgtc
    16921 aaatttcatt tctttgtata attgttttaa ttcacgtttt ctatccatga ttttagatac
    16981 ctctctttat aattgatatg taatatgtaa gtgtaaaaat atattacata tcaattataa
    17041 agagattaat tttatatgta aatagttttt ataaatttaa ttatgaatta gtcatcaata
    17101 aacaagaaat ttagctgtaa aaatccatta taaaggctat tataaatttt aatatgctaa
    17161 aaatataaaa agaatagttg acagtgtaga aaatatggaa taaactaaaa gtgtaatagt
    17221 aatatgaagt tatatattac attgaaaggt gtgaatgatt attatgaata aaaaaataga
    17281 attaataggg aattttgcaa gtgattggat taggtatagt gattatgaat ataaaattac
    17341 tgaagaagat gaattatata tagttccaac agaagatgct gtgtttacca tgtataaccc
    17401 atttgatgta gcagatgata taatagtaga tataattcgc ataggacaag aggcattaca
    17461 agatatatca aaagagacaa aagaaatgaa actaaagaaa gaagtacttg aatttgtaaa
    17521 aaagtatgga ttattgggtt taatatattc aagtgtatat aatagagata tagttggaga
    17581 agaaagagta ctgatgattg aaaagaatta tattacaaaa gagaatatat tgagtacaga
    17641 tgaatatgta aacatgttta taccatttgc aagtgatgaa gatgtgtatt ttaaaaagta
    17701 tagaaggggt atagatgtat caaagagaga agaatctcca aagttttttg gaaagagacc
    17761 attgatttta gatttagtat tttcaaagtt ctacactgaa caactagatt ggattgtaag
    17821 ttttgctaaa gatatgtgta agcattttaa tcaaatttgt gtatataaaa atacatctaa
    17881 tctattaact gaaagtgtta ctattatgtc tggagggttt aatcctcaaa agataggatt
    17941 ttctataagc caattagata aaccaattat tgcatgggag tttgattctt tgaaaacagc
    18001 tattgagact atctatgcct ttgcaattac aagtgaacct tcttctttaa ataaatgcaa
    18061 gtactgtggt aaaatttata ttcctacaaa ttctaaatct caatactgtt caccttcttg
    18121 tagaaattgc tcaaatgtaa agaaaagtag aagtagagcc aaaaaagata aataaaatta
    18181 ataaatatat cattgtttag attttagtaa ataactactg aattaaaagt tgttatagaa
    18241 aattatatat ctaaatgatg gtattttttt gttgagaaaa ttgaaaagtt taattgagat
    18301 agtaattttg agttgacaaa aaccactttt aacactacta ttataagtgg ttggtaaaat
    18361 aatatacatt aatttaagta tagcataaaa aacaaaaaaa gtcaatggaa aaaataaaaa
    18421 aggagaattt aaaaatgata tcacctatta ttttgagttc tataaatcag aatttaaagg
    18481 aaatagaaag aaatgaactg cttgaaacta atatagaatc tggagattat ggtctagctc
    18541 tttctgaatc tgatgtaaaa gatataataa attcgagaga taatacttta aaggggtatg
    18601 gaagaataga attagatata aaagttacaa aacagttaat agaaaatata tatacatcac
    18661 aatatacaaa tgtggacaat tatttagagg ctattaatga tatgcaagaa attttttatt
    18721 atttaaaaaa tgaaactgat gacaagatat gtgatgatga gattatagaa atactgggtg
    18781 aattttatga aaaattttca ggcaatatgg acaatgtaag aggagaagca gacgaatttg
    18841 caaagaaatt taaatttgga gaagtttaat atatgatagg agagattttg tatgttaaat
    18901 aatttaagta atattaatat aaacgaaaat aatttaataa agaaccaata ttccatatct
    18961 ctacttaagg aatgtctaaa ttgtaaagtg atagatgaaa gagaagtata taatatacaa
    19021 caagaagtat ctctaatact tatggacttg attaaaaaat atacaaatgg tcaaagcaca
    19081 tcagtaaaaa cagaagttgc cgaaaaactg ttgatttcaa tatggtatgc tattgatgca
    19141 tatataaata agcttggaga tatagaaaaa agagttgtgg agttgactgc taagaatgtt
    19201 aaaaaagtgt accttggagg cattgaaata ttagaggaag agttagttag gctaaaagat
    19261 ttttataaac taatgatgag tagaaaattg gatactaatt caatagcata taatgatacc
    19321 ttgatggagt tatcaagttt ctttaaaagt tatattgtaa aatttgaagc tcaggacgtt
    19381 ccagtaagta tagattatcc attggcacta gatgatatgg atgttaaggg tatttattat
    19441 gtgaagaatt atattgagag tatagatata gaaaatagat tttgtaacct atttaagaaa
    19501 aaagatagag aaaaattatt ttatgactat gggatgactt ataaaataga ttgtgatgtt
    19561 atacttgtaa atttatttga acttattatg aataattgta ttttttctac tattttagga
    19621 aatgaagtga ctaatttaga aatttcaaaa tatgagtatg aatttttaga aaaacaattt
    19681 aaaaatatgt acaataatat tactgaagat aaagttatag actctgaaga aagtaatata
    19741 attgaattga ttgaagtaga tgatagtttt aacactgaag tagatagtaa tttggattat
    19801 aatgattttg aagaaattaa cagagaaatt agaataaata aaaaaataac tttactactt
    19861 atggaagcaa ttgaagtatt aataaaaaga ttaagcatag aagatgaaga tgtaataaga
    19921 tatattaaaa aatatgaaaa gatatttatt gaatctgtgc taagctcaat aaaaagtaac
    19981 acttttaaaa gtatggtaac tgtgagtaaa aataaaaagg aacacttaga agattatatt
    20041 gttgatgatg aaagtaaatt ggatgatgaa tcgtttagag agatattcaa tgagatatta
    20101 gatagtacat ctataattga aaaaataaga ataataaagg aaaatatcaa tgctaaaaaa
    20161 gactttatcg atattcttga atctgaatgt ttatttggag aagagtattt aatgttgttt
    20221 gcatcattaa gtgaattaga attggcaata ttgggtagtg tagtatttta tgaagatatt
    20281 agaatgagag aaattaatat attagaattt atgttaaatg agaaaactga aactacttta
    20341 tggaagatag agtacattaa atttatgaag aggcaaagtg aagataagat atactccata
    20401 gaagagcata tgaataaaat taattaacaa aaaattgtga ataaatagaa tgttagtata
    20461 taatataatc tgtaaagcat atcaaaaata catatatttg gaggaaaatt atgaaagagt
    20521 atatcataaa taatgttgaa gaaataagag aagaattaat tgatttatct aagaaaatat
    20581 gggaaaatcc agaattagcc tttgaagaaa agtatgcaag tagcattcaa aaagaatacc
    20641 taaagtcaaa aggatttaaa attgaagaag tagaaaattt acctactgga tttatagcaa
    20701 gttttggaga gggtaaacct gtgattggca tacttggaga atatgatgct ttaccagagc
    20761 tatcacaatg tgtaagtgca gaaagaaagc cattggtaga aggaaaagct ggacatggat
    20821 gtgggcacaa cctgttgggt gtagcaggtg taggagcagt tgtttctata aaaaaattaa
    20881 tagaagaaaa taaatttaat ggaacaataa aatattttgg ttgtcctgct gaagaagaag
    20941 gtggaggaaa gacagttatg tgtataaatg gctgttttga tgatgtagat tgtgcattta
    21001 catggcatcc ttttgatata aatgcacctt ggagaggtgg aagtctggca aacttatctg
    21061 taaaatttaa atttaaaggg ataacagccc atgcagctca agctcctcat aatggtagaa
    21121 gcgcacttga tgctgttgaa attatgaatg ttggagctaa ttatttgaga gaacatgtta
    21181 ttgactctat aagaatgcac tatgttataa ctaatggtgg tggaagacct aatgtagttc
    21241 ctggatttgc tgaaagttgg tactttatta gaggtaaaaa agcaaaggat gcagaacacg
    21301 ttcttgatag attaataaaa gtagctcaag gagcagcaat gatgacagaa actgaaatgg
    21361 agtataaggt tacagatgga atatatgact atataccaaa ccaatgtttg actgatttag
    21421 tttatgacaa tatggttttt gttggttgcc ctaaaaatac tcctgaagaa gaagaatttg
    21481 caaaaaagtt gtgtgatact cttactagag aagaaagatt aggtgtagca acatcattac
    21541 aaaatgataa atctattgta gaaagctata tacacaagga aatagttgac ggtgacaaag
    21601 ataaaggact agctggttct actgatgttg gagatgtaag ttatgtaata ccagtggctc
    21661 aatttgcaat ggcagcttgg cctgttggaa ttgcaagtca tacatggcag tcatgttcat
    21721 cagcaggttc aaatatagga ttttctgcta tgataaattc agctaaagta ctggcttgta
    21781 gtgcttatga tgtatttatg gatactaaaa ttattgatga agcaaaaatt gaatttgata
    21841 aatcattaga tggacaaaaa tttaaaccat tggtataatt ttgctaataa aatatttctg
    21901 gggggaatag ctatgagaga aaaagttgaa aaagtgcttg aggaaaagat aaaaccagta
    21961 ttacaaagag atggtggaga tgtagagctt atagatgtaa atgaaaatgg tgtggtttta
    22021 gttagactac aaggggcatg tagtgggtgt ccaggagtga ctatgacaat caaagctatt
    22081 attgaaaatg ttttggtgag tgaagttcca ggagtaactc aagtattagg tgtttaatag
    22141 ttagcgttta ttaaatgtat gttatacata agtatttaat aaagaagtaa aaaaaatagt
    22201 gaagcaatat atatgaaaaa gtatgagaaa gataataaaa cattatagaa ataatgaaat
    22261 atttcatatg tatatgaaga aataagaaaa aatagtgaag caatatatat atgaaaaagt
    22321 atgagaaaga taataaaaca tttatagaaa taatggaata tttcatatat acatatgaag
    22381 aaatagtaaa acaatatata tatgaaaaag tgtgagaaaa taataaaata ctatagaaac
    22441 aattaaatat tctatatagg tattaagaaa catgaaagaa ataatggagt attataaata
    22501 aatatgtaaa agtgtgaaag aaggatgtag ataaatttgt cttatacaaa tttacctaca
    22561 gtatttctta cacacttttt attttactat ccagccatac caataaacat acctataaaa
    22621 tatggaacta accatatagt ccatacaact atactaaact tatgaaagaa ttttttcttt
    22681 tcctcatcat ctttgtatag gacaaaagta gcccaacttg catgaaaaag cataagcaca
    22741 attgcaagaa gacctgtaat actatgaagg ttttgtgata aagcagattt tacttcaaaa
    22801 gtatggctgt tggctatgtt actcattgta aatgttccta aagtatcaaa tataaaacca
    22861 agccaaaata taattacatg tttctttttt aaaatttttg cctttctttc tccaaataca
    22921 cctatagtgt agaaaattaa agctgatgtt ataaatacta ttgcaagtat taatttataa
    22981 ttcataatta tctctccttt acttttgcca atatatttct aagatttttt actaagtcac
    23041 tcaattcgtt ttcatcacaa tttttaaaaa tatcatcaaa actttcttgc attttaattt
    23101 taaaatttgt ggcaaattca aatcctgttg ttgtaaactt aacgtatgta tttcttttat
    23161 cattagattt cttgaatttt tctatatatc caatctgttc taatctgctg attatgcctg
    23221 aaaccgtacc tttagctaaa gacatttcat cacatagttg tgatattgta agttcttgat
    23281 tatgagctat cagttttata actataatct gttggtgtgt taatccattt tctttaaaat
    23341 tttcttctac tgtgtacata gttttagaat aaagttcttt taagagcatg gctattctaa
    23401 aacagtcata attacttttc aataaaatca cctcttaaat ggtttgcata caaactaata
    23461 taatatactt ttcttatata gtcaacctaa aattttataa ttttatagaa aataatgaag
    23521 aatagaatat aaaaatttta ttttaggtat aaataaaata atttgatgaa attttaacaa
    23581 tttttaaaaa gtttgtttac acagttaata aatgatgcta aaattaactc atagataaat
    23641 atagaaaatt taattttgtt taatgataat ggaaccacga aggttttaac gttactttac
    23701 gtttgctttt atgtggtttt ttttattgca acaaaggggg gttggggttt gttaaaggat
    23761 atactgaaaa agttgttgca gtttatttta gtaatgtttt tattatcttt tattgttttt
    23821 tacatggcaa gacttgctcc aggagaccca ttaatctctt actatgggga tggagtagaa
    23881 agaatgagta cacaagaaaa agaaaatgct atgaaaaaac ttggtttgga tgaaccaata
    23941 tacagtcaat atataaaatg gatttcaaat gcatcaaaag gggaatttgg aatttcattt
    24001 aaatataaac aaaatgttac ttctgttata aatgatgtct atataaacac tataatatta
    24061 ggtggttcag gatatatact tacctttgtg ctagccttat tgttaggaat attttgtaca
    24121 ttacatgaag ataagctcat tgatagaatc atatgcaaat taggaacaat aaccaactgt
    24181 attccatcat tttgggttgc cttagtactc atacttatat ttagtataaa cttaagtata
    24241 cttccaagta gtggtgctta ttcaatggga gaagagtcca gtatattaag taggatatca
    24301 catctaatct tacctcttac agtacttata ttaagccatc tatggtatta cacatatatg
    24361 ataagaaata aattactaga ggaaataaga gaagattatg tgcttttatg taaagcaaaa
    24421 ggtttaaata atagaactat tgtttttaag cattgcctga gaaatataat gccatcatat
    24481 attagtataa tggccatttc gatacctcat atactaggag gaacttatgt agttgaaaaa
    24541 gttttttctt atcctgggtt aggaacactt tgttttgaaa gtgcaaagta tcatgattat
    24601 aatatgttat tagttttatg cttaataaca ggggctttag tagtatttgg gaatatgtta
    24661 gctcaaatta taaacaataa aatagaccct agaatgaaat atgatagagg tgatactaat
    24721 gaagccacaa tatagtgatt ttgaaattgt aggagaaaat tatattttag tagcagaaga
    24781 tgaacatata gaaacaaaga aaaccttatc taaaaaaatt aaagaattac catatatatc
    24841 aattattatt ttatcaatca tagtcattgg aagtgtattc tcatctttta taatgacaca
    24901 tgaaccaaca tacatggatt tagcaagttc taatttagct ccaaataaaa cttttttatt
    24961 tggaacagac tcaatgggta gagatatata ttctatgata tggtatggag gaaaaatatc
    25021 tttatttata ggactatttt ccactgtaat atcaactgtt ataggaattg tttatggtag
    25081 tataagtggt tcagcatctg aacttgtaga tgatgcaatg atgaggttta cagaaatcat
    25141 acttagtata ccttcaatat taattataat atttgtacaa gctatacttg gaaattcaaa
    25201 tccagtatct atgtccatag taatagggat aacaagttgg atgaatatat ctaagatagt
    25261 gagaacagaa gttagacaaa taaaaaatag cgaatacata ctagcagcta agagcatggg
    25321 aggaggattc ttctatgtat taaagcaaca tttacttcca aattttgtgg catcaataat
    25381 gttcatggtt gtaacaaaca taggcgcagc tattggtact gaatcgacac ttagcttttt
    25441 aggaattggt ttaccaatag aaattgtttc ttggggaagt atgttatctt tgtcagagga
    25501 agcattactt tcaaatagat ggtggattat actgattccg ggtatatttt tggttaccac
    25561 attggtgtgt ataacaaata taggtaatta tataagaaag aataataata aaaaatctag
    25621 taatttataa atcagcattt gagtattaaa taagcaaata ctgaggtatt tgaaatatct
    25681 atttaaataa atggggtcaa agctttgaaa aatattttat aaaaagcaaa agggggagca
    25741 tcatgaaatt aaagaagtta aaagttttaa gtttagtaat gatacttagt ctaatggcag
    25801 gttgttctag tggaggagac aaagataaga aggcagatac gcctaaagat ggaaaggtgc
    25861 ttgtttatgg aagtaatgac tatacaagta taaatcctgc actatatgaa catggagaaa
    25921 taaattcgtt aatatttaat ggattaacag ctcatgatga aaataataag gtggttcctt
    25981 gtctagcaaa ggactggaaa tttgatgagg caacaaatac atatactttt aatttaagag
    26041 atgatgttaa gtggcatgat ggagaaaaat ttacagcaaa tgatgttaag tttacaatag
    26101 aaactataat gaatccagat aatgcttctg agatagcatc aaattatgaa gatataacaa
    26161 aaattgatgt ggttaatgat aatactataa aaattacttt aaaagctcca aatacagcaa
    26221 tgcttgatta tttaacagta ggagtattac caaaacatgc tttggaaggt aaggatatag
    26281 ctactgatga atttaatcaa aaaccaatag gtactggacc atttaaactt gaaaaatggg
    26341 acaaaggtca aagtataaca cttgttaaga atagtgatta ttttgtaaaa gaaccaggat
    26401 tagataaagt agtctttaag attgttccag atgataaggc taaggcaatg caattaaaat
    26461 caggagaatt agacctagct caaataactc ctaaagatat gtctaacttt gaaaaagacg
    26521 aaaagaattt taaagtaaat ataatgaaaa cagccgacta tagaggtata ttatacaatt
    26581 ttaattctaa attctttaaa gataaaaaag ctaaaggatt accaaatgct ttaagctatg
    26641 caatagatag aaaagcaata gttgacagtg tattattagg acatggtgta ccagcatatt
    26701 cacctttaca aatgggacca tataataatc ctgatataga aaagtttgaa tataatccag
    26761 aaaaagctaa acaagaaatt gaaaagctag gttggaaatt aggttctgat ggaatctatg
    26821 aaaaagaagg tactaagtta gctttcgaga taacagcagg tgagagtgac caagttagag
    26881 ttgatatggc taaaatatgt gcacaacagt taaaagaaat tggtgttgat gctaaagctg
    26941 ttgttgtaac tgaaacagat tgggctaatc aagatgctca tttaataggt tggggaagtc
    27001 catttgaccc agatgaccat acatataaag tatttggaac agataaaggt gcaaattaca
    27061 gtgcttactc taatcctact atagataaaa tacttcaaaa agcaagagaa acagaagata
    27121 aagatgaaaa attaaagtta tataaacaat tccaagtaga aatgactaag gatatgcctt
    27181 atacatttat tgcttatata gatgcaatat atgttggaaa accaaacata aaaggattaa
    27241 cacctgatac agttttaggt catcatggtg taggtatatt ctggaacata gctgactgga
    27301 caattgaata atactgaaat taattagaaa ccttagggat aaaaatatat ccctaaggtt
    27361 tgaatagcat aggggttgat ggtttttgga acatttattg gaggttaata atctatctgt
    27421 aagttttaaa gtagaagaag gagaagttca ggctgtaaga aatgtatctt ttaatctaaa
    27481 aaaaggtgag acattggcta tagttggaga atctggttgt gggaaatctg tactgtgtaa
    27541 gagtttgatg agggtactcc catacaatgg atatattaaa aatggagagg ttttacttaa
    27601 atcaagtgat ttggttaaga agtctgaaaa agagatggaa gatataagag gaaagaatat
    27661 atcaatgata tttcaagacc ctatgacatc tctcaatcca actatatcaa taggaaagca
    27721 aattgcagaa gctgtaatca ttcatcaagg tataagtaaa agtgaggcaa aaaagagagc
    27781 catagagctt attgaattgg ttggaattga taatcctgag aaaagattta aacaatttcc
    27841 acatcatttt tcaggtggaa tgagacaacg tatagtaata gctatagcac ttgcttgtaa
    27901 tccagatgta ttgattgctg atgaacctac aactgcttta gatgtaacta tacaagctca
    27961 aataattgat ttaataaagg atttacaaca taaaataggt ttgtcaataa tatttataac
    28021 tcatgattta ggagtagttg caactatagc tgatagaata gcagttatgt atgcaggaaa
    28081 gattgtagaa ataggaactg tagaggatat attttatgac cctagacatc catatacttg
    28141 ggggttgttg ggttcattac ctactttaga ttcgcaggat gactatttat ataatatacc
    28201 tggaatgcct ccaaatttat tgaacccacc aaaaggagat gcttttgcaa taaggaataa
    28261 aaatgcactt aagatagatt atgaaaaaga gccaccaatg tttaaaatta atgatacaca
    28321 tagtgcagca acttggttat tgcacccaga tgcacctgaa gtagatgtac cagttagagt
    28381 aaattgtgga agggtgattt ctaatgaata gagaaaaaat actggaaata aaaaacttaa
    28441 agcaatattt tcatttggac aaaagtacta ctgtaaaagc agttgatgat ataagttttg
    28501 atatatataa gggtgagata tttggattgg taggagagtc gggttcagga aaatctacaa
    28561 ctggtaaaac tataataaga ctacatgaat ctactggtgg agaagtaatt tacaaaggaa
    28621 attgcatatc agataaaaaa acgtacaaat ttataaaaaa agatgtaaac aaaagtatgc
    28681 agattatttt tcaagattct acttcatcat taaatccacg tatgacaatt gcagatataa
    28741 tttcagagcc actaaaaatt caaggtatct gtaaaaataa aacagataga atgaataaag
    28801 tatatgagat gctaaagcta gtaggacttg atagaagtta tgcaaataag tatccatctg
    28861 atttttcagg tggacaaaga cagcgtattg gcatagcacg agctctttca gtagacccag
    28921 aatttataat agctgatgag cctatagctt ctttggatgt gtctatacaa gcacaaatag
    28981 taaatttatt taaaaaattg caacaagaaa agaatttaac ttgtttattt atagctcatg
    29041 atttatctat ggtgagacat attagtgacc gtataggagt tatgtataat ggaaaattag
    29101 tagaattggc agattcaaat gaattgtata ataatcctat tcatccatat acaaaatcac
    29161 tactttctgc aattcctgtt cctgacccta gatatgctaa gtcaagaaat agaatagagt
    29221 ataattctaa tggatatgat tgttcaaatg aaaagtcatt aagttggatt gatgtttcag
    29281 atggacattt tgtatattct tctaaatcag aaataaataa atatcaacaa aatttaaaag
    29341 ttgtttagta taaaaatact tgaacaggta tttatttgaa atacaaacta aaaccctatt
    29401 tttggagaaa ttttaaaact actccaaaat agggtttttt atttctactt ttcaaatata
    29461 ataaattcaa atataataaa attaaaaaca agtaaagtat gttgttaaga aagtactata
    29521 aaattaaaaa tagtaatgta gaaaaaatga gcaataatct tattttttct acaataattt
    29581 tctataattt aaagtaagga aagtttgata aattataacc ttagttaaag aataagagtt
    29641 tattaaaaaa taaagtgtgt ataggggttt tagttaataa aatactataa tgtaggggag
    29701 cttgtaatga tatataaaaa agataaagta atgaaagttt tagaatcatc atatagggaa
    29761 aatgaaaaag ggattacagc taaccaatta tcagaaaaga caggaattgc aagaaacaat
    29821 ataagtacat atttaaacca attattcaat gagggtatgg ttacgaagat aaaaggaaga
    29881 cctgtgtatt atatccctgc aaatcttaac cttaacaaga atgaattgct tgatacacaa
    29941 agtgagttgc ttggcataaa aaaaggaaca aaagtagaaa acaaaataaa aaaagatagt
    30001 tttgaaacac taataggtaa agaacatagt ttaagaccta taatagaaaa atgcaaggtc
    30061 gcaatgctct atcctacaaa tggacttcat actattatct atggagaaac aggagtagga
    30121 aagagtatga ttgctagata tatgtacgat tattctatag atagtggaat tagaaaaaat
    30181 aaagctcctt ttgtaacatt taattgtgct gattatgcta ataatgcaca attacttatg
    30241 ggacatattt ttggggtaga acaaggagct tatacaggtg cagaatcttc cagaaaaggt
    30301 cttttggaaa ttgcaaatgg agggatacta tttttagatg aaattcatag actaccagca
    30361 gaaggtcaag aaatgttgtt tacttttata gataagggaa catttaaaag actaggagat
    30421 gcaacgaaag aaagagagtc taaggtatta ataatatgtg ctactacaga aaatccgaca
    30481 tcaacactat tagatacatt caatagaaga ataccaatga aaatagaaat accatcactg
    30541 agagatagga caatgattga aagaatggaa ttagtcaaaa attttttcaa ggaagaatca
    30601 aaaagtattt ctatttcaat aaaagtacat aaagaaataa ttaaatcact attactttat
    30661 gattgtaaaa ataatgtagg tcaacttcaa aatgatataa aattagctgt tgcaaatgga
    30721 tacctaagat ataaaagaga taataaagaa ccagtttata tagagaaaaa atattttgat
    30781 aaatctataa atgaagtgag tgatgattat agagagaagt ctattaaagc agatgaagtt
    30841 ataacacatg gaatagaata ttttgtattt acgtcattag gagaagaaga agttattaac
    30901 tacaatgata taaatattat taatgcagta aatactaatc ttaataaatc tgtagaaatg
    30961 aatatagcag atgcttctct atttaataat gttaagttta aaagcatgtg tgaatcaata
    31021 agacaaatta ttaaagaaga tgaagaaata gacataagtg aatcaatatt taagtcaatg
    31081 gcaatttata taaaaagcgt attggataaa agtagctcta caaacaacat agatttgaat
    31141 caaattagaa gaaataacag aagtgaattt aaaacagcac taaaaatagt tggaataatt
    31201 gaaaatgaat ttgatttatt tttatctata gaaactgtag catatattac attattcata
    31261 gtacaagcta aacaagatga acaaaataaa atagtgaata acattaatat tatagttgct
    31321 atgcatggag aaactacagc aagtagtatg gtaaaagcag ttggtgatat tattggaaag
    31381 tgcagtgcta tttctttcga tatgaagtta gataaaagct atgatgaagt tattgtagat
    31441 tttaaaaatt taattaataa tatagacaat aaagatgtat tactatttac agatatgggt
    31501 tcattaaact cttttgatga aattataaag aaagaaaaaa agtgtggagt aagagtaatt
    31561 cctatggtga caacattaac tgtgttagaa gcagttcaga aggccaatat gggattacct
    31621 ttaaatgacg tatacaactc tataactaac acgagaaagt actattttgg aactaatgaa
    31681 attcaaaata aagaaaatct gtctaaaaca attattatag catcacatgt ttcagaaggt
    31741 gtagataata aaacaagaaa aatattagaa gaaaagatga gtaggtactt agatggaata
    31801 gatattatat ctgtcccata taaaacagaa aaagatttaa gtttaaatat tacgaaactt
    31861 aaagaaagta gtaatattgt tgcagtaatc aatgaacaaa gaattaatat tagaggaatt
    31921 gactatatta gcaaaaaaga tatagataag gatgaaaata tcaataaatt aaaaaatatt
    31981 ataaagataa gtataggtta tgatgatgta gttgaaggtt taaagacatc cttaaaaagc
    32041 tctaattata ataggatatt taaagatata aaatatgtgt ctgatgaatt gtttttagta
    32101 tttaatatag aaaagaaata cgataaagta attggactta tgatgcattt agctttcatg
    32161 gtagatggat tgattggaaa tactagagaa attgaaaagc ttgataaaga aaaaacactt
    32221 gattatcaca agagcctaag caaaataaaa gatatagtat ctcaattaga taaaaaatat
    32281 aatatagaaa ttaatgaaaa agagtgctat caaatattat taatattaga atatgcagaa
    32341 ataatcgaaa aagattacca atgagtataa aaacttattt ggtaattttt tttagtctaa
    32401 aataatatta atattagaca catataaata tacttagaca aagtatctaa gttttgatga
    32461 taaaaatgat tgaaataaca gtgttttaaa gtgtctaaaa aaactggcat aatacttgct
    32521 tatattatta aatgagtaat ataaataatt aaaatttaaa taaaaaggag taaataatat
    32581 gcaagataaa atattaagag ataaaaaggc aaatgaccca tgggcaagaa tgactacaag
    32641 aaacggattt agtgcagatg aaataatatc gtcatttcaa aaagctataa gaagaaatat
    32701 ggtggaagaa gcttgtgaat ttgcttatga actatatata tcatcaccag aattagaaga
    32761 taagctatgg agaaggatac ttacaatatc agttgaagat ataggaatgg gggacccatc
    32821 agctgtaata atcataaata atttaaatca agtaagaaag gaatattcat atgcagatgg
    32881 agacagacct ttatttttca tacatgcaat aagatatctt tgtgcatgtg agaaagatag
    32941 atcaagtgat ttattaaaaa atataattat aaaaggattt gcaatgggta aagttccaga
    33001 agttatggac gtggcacttg acaagcatac atatagagga ttagaaatgg gaagggattc
    33061 attccacttt ttaaatgaag ctagtatagt aataccagaa aaagaagtag ataatgatta
    33121 taaagaaaga tacttaaaaa tattagaaaa atataagcaa gaagaagtta tagatacggc
    33181 tttcaagttt aatcattggc agtactaaga attagagtaa atattaataa gaattaaaat
    33241 aaaataataa gaattaaagt aaaaactgat aggaattaga gtaagaattg gctgataaaa
    33301 aattagaaaa taaaagaggt tagatattgt aggagggtaa ttatgaatat tttattagta
    33361 tgtgcaaatg gagcttcaac aggtgtactt gtggaaaaga tgaaaagttt ttgtagtgag
    33421 catgaaaagt taaaaactaa aactataaat atagaggcaa catcatttga aaatttaaaa
    33481 tcatacattg aagctaatga taccgatgta gtacttgtag caccacaaat taggtttaag
    33541 gaagatgatg tagttgaagc ttgtaagaat tacaaaatag cagttggttt aatagatact
    33601 aagcattatg gtcgtatgga tgctccttct gttatgaagt cggcaatcga attatataaa
    33661 aatagataat tggggggaat aaaaatggaa gcacttcaaa ataatttaag aagatttctt
    33721 cttccaatag cacaaaagat tgaaaaacaa aggcatcttc aagctataaa agaaggtatg
    33781 ataagcatta ccccaataat aattgttggt tctttaagta tgttatttat ggcattaaac
    33841 aatatgttac ctgaaggaag tgcaaaaaca ttattatctg aaaatatgga tacattactt
    33901 atccctaata aatttactat gagtttatta tctatatatt cagcattttt tatagctcag
    33961 gcactagcta aaaaatataa tcttaatcat gtagaaatag gtatgacagc agttgttgca
    34021 caacttgttg tatgtggaca agtagtagat ggtgtattag atacaagtta tttagatgca
    34081 caaggtttat ttgtaagtat attagtagca cttttagtag ttgatattac taagtttatg
    34141 aatgataaaa atctcgtaat tagatttcca aaagaagtac ctagtgtagt aaataaaagt
    34201 tttagaaact taactccaat gatagtatgt ataatgttat ttacagcaat agcagcaata
    34261 actaaaaatg tctcaggtca accattacca gctattataa tgaatttctt agcaccagct
    34321 ataagcagtg tagataatgt ttttgcagta actataatac tatttataac tcaattatta
    34381 tggttctttg ggcttcatgg agcagcaata acttcaagta tatggatgcc aatagcagct
    34441 acatatatgg cagaaaatgc aacgttaata gcagcaggag gagacccaaa atatgtgttt
    34501 acaataggtt tttactatgg tttcttgcaa gtaactggtt cagggataac tttgggatta
    34561 gtgtacctta tgtcaagaag taagtgtaag agttttaata gtatgggaaa agttgtgatt
    34621 ttaccatcac tatttggaat aaatgagccg gtaatatttg gaacaccaat agttatgaac
    34681 ccatatatgt ttgtaccatt tgtatttgga cctgtattgg ttggagcact taattttatg
    34741 gcattaaaag taggattagt aggattacct attgcagaac caccaggctt tttacctcca
    34801 ggggttgcag catttttgat gacattagat tggaaggcaa tcgtattagt atttgcaagt
    34861 ataatactta tgaccttgat atattatcca ttctttaaga taatggagaa ggaagaatta
    34921 aataaaaatg cagaattagc tacaacatta gatgatgata gctttgattt ttaacaagtc
    34981 attagaagga gaaggatatg gtacctgaaa tggaagaaaa agtatttagt ataatatcag
    35041 aagcaggaga tgctaaaagt gatgtaatga tgtctcttaa ggaaataaaa aaaggtgact
    35101 acaataaagc aaaacacctt ttaaattcag cctcagagaa aatacaaaca gcatcaaagc
    35161 atcatttaga attactatca aatgctatga atagtgaaga tagtgggact gattttttag
    35221 ttgtgcactc tgaagaccat tattcaaatg cattatttgc tcattcttta gtatctgaac
    35281 ttgtagatat atttgagatg atggacccaa gaattaataa gaattaaatt tcttgtaaat
    35341 agtgcttgaa gtttggctat agtttattaa atcaatagat ttaataaatg aactcccttt
    35401 tactttaaag tactagtatt tataaattta tttataaata cagtaaattt ttaaatagaa
    35461 aaggagatat tattatggaa aaattttcag tagtaatagc tggaggagga agtacatata
    35521 ccccagaaat catattaatg ttgctagata acttagatag gttaccatta agagcaatca
    35581 aattatatga taatgatgaa gaaagacaaa ataaggtagc taaagcttgt gaaatactaa
    35641 taaaggaaaa ggaccctaat atagagtatt tagcaacaac atgccctaaa gaggcttata
    35701 cagatgtaga tttttgttta gctcatataa gagttggaaa acttgaaatg agagaactag
    35761 atgaaaagat acctctaaaa catggggtag ttggtcaaga aacttgtgga ccaggtggaa
    35821 tagcttatgg aatgaggtct atagaaggtg ttcttgaaaa catagattat atggaaaagt
    35881 attctccaaa ttgttggatg ttaaattatt ctaatccagc atcaataata gcggaagctg
    35941 ttagaaggtt gagacctaat tctagggtaa taaatatttg tgatatgcca ataggtatgg
    36001 aacataatat agcaaggatt gcaggactca aatctcgtaa acatatggat ataagatact
    36061 ttggattaaa ccactttggt tggtatactt caattaaaga taaaaaagga aatgaacttt
    36121 taccaaaatt agtagaacat ataaaagaat atggttttat aaatggtgaa gaaggaatga
    36181 aaaatgacaa gaaagatagt tggtttgaaa ctaatttatt tactaaagaa atagtaaaaa
    36241 cagatttaac tacaatacca agtagttact taaagtacta tctatttcca gattatgttg
    36301 taaatcattc tgatgtaaat tatactcgtg ccaatgaagt tattgatgga agagaaaaag
    36361 aagtttttgg ttcatgtgca ttgatagaaa agcaaggaca ttctaagggc agtgggctta
    36421 agataggaat acatgcagag tttatagtgg atttagctac agctctagct tttaatacac
    36481 aggagcgtat gttattgata gttcaaaata atggagctat agaaaatata caagatgatg
    36541 ctatggttga aattccatgt atagttggaa aagacgggta tgaaccatta tcaataggga
    36601 aaataccaac attccaaaaa ggtttaatag aacaacaatt agcagttgaa aaattagtag
    36661 ttgatgcttg gattgagaag tcttataata aattgtggca agcacttatg ctatctaaga
    36721 cagttcctag tgcaacagtt gcaaaagaat tactagatga tttaatagaa gcaaataaaa
    36781 attattggcc aaaacttaat taagaataat atatttttaa agttcctaca gtttagtaca
    36841 tagcctagtt aatgtatttg tagggacttt atttatttac aaatgagata caagtggcaa
    36901 acatagtgta ttttgcagtt aggaaagtag gtttagttac ttgttagttg gattaaattt
    36961 aggtacaata atttactatc aatcaataga aataaccaga gaagttgcat aataaaaaat
    37021 ttacactttt aacaggattt atgtaggtgt ttatagaaat aaataaatat gtacataaat
    37081 acataattat ttttaagtct aaaatacaag aatggagaga tggctatgaa gtggagagat
    37141 tatgctgatg atgtaaattt atttgaggat tttgataaaa ctatcaagcc tctaactgac
    37201 gaacaaagaa gaaaaaatat aaacacttta attgcatatt tttcaaaaga agttccttct
    37261 aaaatataca atttatcaaa tgatgaaatt aagcctagag atatacttag aggacttttg
    37321 aatgtatatc caccaaaaga aattgctcca gagatactta atatgttaca taatttattg
    37381 ttgattgaat gtgaagaaag agagttagta gatgtaaatg atatagaaga aatagaggaa
    37441 gggattgcca tatggagagg taatataacg aatttaagag cagatgcaat agtaaatgct
    37501 gcaaataaca agttattagg gtgtttacaa ccattacatc tatgcgtgga taatgagata
    37561 cattcatgcg caggtcctag acttagagaa gattgtgaca aaataataaa aaaacaaggg
    37621 catttagaat atactggaga tgctaagata actagaggtt attgtttacc tgcaaaattt
    37681 gtagtacata ctgttggacc tattgtatct ggtggtcagc caagtaaaga gcaagaaaag
    37741 caactgcttc attgttataa atcatgttta aatacaataa aagaaattga tgaaataaaa
    37801 aatattgttt tttgtggaat ttctacaggt gtatttggtt atccaaaaaa agaggcagct
    37861 aatcttgctg taagtagagt tagactatgg ctaaaagaaa atcctgaaaa aaatttaaaa
    37921 gttgtattta atgtgtttac tgaagaagaa gaagagaaat atagaagaat atttaaataa
    37981 tattatgtaa cagaatccta aaaatactta ttatataata caaattcatt gtataagata
//
LOCUS       DAELTA010000015        38000 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.015, whole genome shotgun sequence.
ACCESSION   DAELTA010000015 DAELTA010000000
VERSION     DAELTA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.015"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(46..1974)
                     /locus_tag="KPG13_000690"
     CDS             complement(46..1974)
                     /locus_tag="KPG13_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0984434.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2208..2660
                     /locus_tag="KPG13_000691"
     CDS             2208..2660
                     /locus_tag="KPG13_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0984435.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2663..2971
                     /locus_tag="KPG13_000692"
     CDS             2663..2971
                     /locus_tag="KPG13_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0984436.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3085..4191
                     /locus_tag="KPG13_000693"
     CDS             3085..4191
                     /locus_tag="KPG13_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0984437.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4266..6944
                     /gene="mngB"
                     /locus_tag="KPG13_000694"
     CDS             4266..6944
                     /gene="mngB"
                     /locus_tag="KPG13_000694"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG0984438.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7261..9594)
                     /locus_tag="KPG13_000695"
     CDS             complement(7261..9594)
                     /locus_tag="KPG13_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0984439.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9569..10285)
                     /locus_tag="KPG13_000696"
     CDS             complement(9569..10285)
                     /locus_tag="KPG13_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984440.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(10699..11355)
                     /locus_tag="KPG13_000697"
     CDS             complement(10699..11355)
                     /locus_tag="KPG13_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0984441.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(11410..12039)
                     /locus_tag="KPG13_000698"
     CDS             complement(11410..12039)
                     /locus_tag="KPG13_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0984442.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(12170..12811)
                     /locus_tag="KPG13_000699"
     CDS             complement(12170..12811)
                     /locus_tag="KPG13_000699"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG0984443.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(12820..13491)
                     /locus_tag="KPG13_000700"
     CDS             complement(12820..13491)
                     /locus_tag="KPG13_000700"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0984444.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(13496..14380)
                     /gene="rsgA"
                     /locus_tag="KPG13_000701"
     CDS             complement(13496..14380)
                     /gene="rsgA"
                     /locus_tag="KPG13_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0984445.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(14478..15215)
                     /locus_tag="KPG13_000702"
     CDS             complement(14478..15215)
                     /locus_tag="KPG13_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0984446.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(15323..17338)
                     /gene="pknB"
                     /locus_tag="KPG13_000703"
     CDS             complement(15323..17338)
                     /gene="pknB"
                     /locus_tag="KPG13_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG0984447.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(17355..18107)
                     /locus_tag="KPG13_000704"
     CDS             complement(17355..18107)
                     /locus_tag="KPG13_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG0984448.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(18127..19158)
                     /gene="rlmN"
                     /locus_tag="KPG13_000705"
     CDS             complement(18127..19158)
                     /gene="rlmN"
                     /locus_tag="KPG13_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG0984449.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(19166..20491)
                     /gene="rsmB"
                     /locus_tag="KPG13_000706"
     CDS             complement(19166..20491)
                     /gene="rsmB"
                     /locus_tag="KPG13_000706"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG0984450.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(20553..21254)
                     /locus_tag="KPG13_000707"
     CDS             complement(20553..21254)
                     /locus_tag="KPG13_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG0984451.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(21323..22075)
                     /locus_tag="KPG13_000708"
     CDS             complement(21323..22075)
                     /locus_tag="KPG13_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG0984452.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(22085..23014)
                     /locus_tag="KPG13_000709"
     CDS             complement(22085..23014)
                     /locus_tag="KPG13_000709"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG0984453.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(23029..23469)
                     /gene="def"
                     /locus_tag="KPG13_000710"
     CDS             complement(23029..23469)
                     /gene="def"
                     /locus_tag="KPG13_000710"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0984454.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(23487..26012)
                     /gene="priA"
                     /locus_tag="KPG13_000711"
     CDS             complement(23487..26012)
                     /gene="priA"
                     /locus_tag="KPG13_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG0984455.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(26251..27450)
                     /gene="coaBC"
                     /locus_tag="KPG13_000712"
     CDS             complement(26251..27450)
                     /gene="coaBC"
                     /locus_tag="KPG13_000712"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG0984456.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(27455..27721)
                     /locus_tag="KPG13_000713"
     CDS             complement(27455..27721)
                     /locus_tag="KPG13_000713"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG0984457.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(27724..28341)
                     /gene="gmk"
                     /locus_tag="KPG13_000714"
     CDS             complement(27724..28341)
                     /gene="gmk"
                     /locus_tag="KPG13_000714"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG0984458.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(28371..29252)
                     /locus_tag="KPG13_000715"
     CDS             complement(28371..29252)
                     /locus_tag="KPG13_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG0984459.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(29256..30086)
                     /locus_tag="KPG13_000716"
     CDS             complement(29256..30086)
                     /locus_tag="KPG13_000716"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG0984460.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(30373..31407)
                     /gene="ltaE"
                     /locus_tag="KPG13_000717"
     CDS             complement(30373..31407)
                     /gene="ltaE"
                     /locus_tag="KPG13_000717"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG0984461.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            31703..33478
                     /locus_tag="KPG13_000718"
     CDS             31703..33478
                     /locus_tag="KPG13_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG0984462.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(33693..35345)
                     /gene="abc-f"
                     /locus_tag="KPG13_000719"
     CDS             complement(33693..35345)
                     /gene="abc-f"
                     /locus_tag="KPG13_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG0984463.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(35644..36855)
                     /locus_tag="KPG13_000720"
     CDS             complement(35644..36855)
                     /locus_tag="KPG13_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG0984464.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(37139..37672)
                     /gene="pyrR"
                     /locus_tag="KPG13_000721"
     CDS             complement(37139..37672)
                     /gene="pyrR"
                     /locus_tag="KPG13_000721"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG0984465.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
BASE COUNT        12572 a         6576 c         4137 g        14715 t
ORIGIN      
        1 aaaaatattt tattaaagtt tttaaaactt atgtaatata aaaatctata ctatagactt
       61 gattatggaa agaaaattat caaaatcatt acaattagat atactatctc taatatcatt
      121 gtcatgaatt atctttaaaa ggatatcata aatggagttt atttttatat aatcatcttt
      181 atttatagcc aacatcatga ttaatttaac agtagaattt tcactccaat atattggttc
      241 attcaatatg gaaatacaaa tcttagttct tatagagctt aattccatag gatgaggtat
      301 agctaaaaaa tcatctatat tggtagaaga taaactttct ctgtaaaata cagattcagc
      361 aaatgatgga aatacaattt cactttgagt taatttgttg cacatcaact ttataagctc
      421 atctttatct ttgattttag gattaacaat aaataaattt ttatcaaaga aattgtccaa
      481 aagattacta taaatatggg aattattagt gattgactta gatatattct ctatatcttt
      541 ttttaatagt tttaaatcca ctaatacaat aggaatacta tcatgattta aaggaattgt
      601 cgatattatt atatctacat ctgacaattc actagctaaa aattgattaa atgatagaca
      661 ttgaagtata tttattttat catgaaaaac tttgtttaat tgaacctcta ataacctact
      721 tgaaccatac cctgaaccac atactaatac tacgttttta cacttaatat tactttgaaa
      781 aaatctctct attccagcac ctatatgcag ggatacatat ccaatttcat cttctgttaa
      841 agagtaaata ctatttttaa aaactttttc tatagcattt aaagttattt caaatgctaa
      901 aggataatta ctctttattg tatttataag gggattcacc ttatttaaat tataagattt
      961 agaattgaga attgatttaa agtgcagtac taaatcatca aataatacac tatcatttct
     1021 aaggtcaaaa gtatattgat tgttgattac ttctaaaatt tcttcaacat actctataat
     1081 tttagtatct accctattgg aaacgttatc caaaagagaa cttttagtta taaaatgatt
     1141 atatagataa actctgtctg cctttgaaat aattatagta tatttactct caatataatt
     1201 aaatatattt tcaacagttg tattcgattg aaaatctgtt aaaacaacat ctaaaacatt
     1261 atctaagcag taaccatcaa gtattctact tacagtaata gctaaatgga taataaagtt
     1321 ttttagatta aaatctagga aattgatatt aaatttatta atttcactta acactaccct
     1381 ttgaagctct attaaatcta cattattaaa taattctatt tctctatctg taattccaat
     1441 tatatagttt tcaaaattcc tatttatcat attattagaa atacatcttc gaatatctat
     1501 ttccttacct tcaactttaa ctccataata tggcttagaa actaatatta aattatactt
     1561 tgacaaaata ggctttattc tttttatgtc atttagtatt gtaaatttac tcacatacaa
     1621 gctattagct aaatcttcta gttttatata tgtattgtta tagagaatat attttaaaat
     1681 atattgattt ctttctatag gagaatctgg tatttcactt gaatccataa tatcatcaga
     1741 tattaatgct agatactgtt ggtataatgc taaattaaga atatctatat aatatccttc
     1801 tccttttttt attttaataa tagcaccatt tttctctaaa gttccattta tagaagtaat
     1861 atcatttctt atagttctct cactcacttt taaatgatta cttaaagttt tacaagtaat
     1921 tttatcatca ctttcattta ataatttaaa caacgtgagc aatctcttgt gcataaatat
     1981 ccccccttat agaatttctt ctaaattata ctatttacac tcatataata caattactct
     2041 aataaaattg tattataata atttatttcc acttctatat ggaaattagt tattatcata
     2101 atataaattt ccatgtcgaa gtggaaatta gttatattta atttaattag gataatgatt
     2161 acaatataaa tataagttaa gacattatga atggagtgga aaaatatatg gtactacaag
     2221 aaaaatatat atttatagat aaagacttaa aaagcaaaga agaagtatta aattttattg
     2281 cagatagtgc ttatatacta ggtattacaa ctaataaaga ggctctttta aaagaccttc
     2341 taaaaagaga aaatgaattt ccaacaggaa taaaagatgg atttgcgata cctcatacaa
     2401 agagtgtaaa tgtaaaggaa gcttccatac tctatattaa aacaactaac gagttagaat
     2461 ggaaaacttt agatgatagt aaggttagat atgtatttag tttgttagcc ccacataaaa
     2521 atgaaggaaa tatacatttg aaaactctat ctaaattagc cacatgttta atggaggatg
     2581 attttacaag tgaagttaaa aatgctagtg atgcaagtca tttagtaaaa tatataacta
     2641 ataaaatgaa ggaggaataa ttatgaaaat agtagctata acatcatgta tagcaggatt
     2701 agcacacaca cctatggcag caaaagcttt agaacaagct gcatcaaaaa tgggtcatga
     2761 aatcaaagta gaacaacaag gtgctatggg acgagtaaat actataacaa aagaagaagc
     2821 acaagatgcg gactttatac ttatagcatc tgaccaaact atatctggaa tggaaagatt
     2881 tgatggtaaa aaagtgataa aagtcaaaat aggtatagcg ttaaaaaaac cagaagctgt
     2941 cttaacaaag tgtatagaag ctatttctta ataaaaaaat ctcactctaa tatagaatga
     3001 gaaaaatcaa aaattaaaaa attgtctaat gtaattataa catattatca aaaaatataa
     3061 caaacttata taggaggatt aaaaatgagt aaaaaaaata aatctctttt aagtgaactt
     3121 aagggacact taatgacagg tatctcatat atgttgccac ttgtaatagg ctcatcttta
     3181 gttgtagcta tcccaaagct tacagcttta gcagtgggta taacaagttt agatgcttat
     3241 aaaaatgtat ctggttttta tcatattctt tacttaatgg aacaagttgg ttggactggc
     3301 ataggtctat taaatacggt attagcagga tttatagcat tttcaatagg tgagaaacca
     3361 gctatgggtg caggttttat tggtgggctc atagcaagta atactaatgc aggattctta
     3421 ggagcagtaa tagcaggttt ctttgctggg tatgtttgta agtttttaaa gaaaaaaatt
     3481 aaaatatctg ggtcagcatc aggaatgatg ccacttataa taatgccact tataacagtt
     3541 ggattaactg gttttttaat gagtgttgta ttagcaggac ctttaggaaa cataaatact
     3601 tcattaaatg cttgggtagc tgaaatgtgt caaaatggaa caaattcggt ggtattggct
     3661 ttaattttag gcgcaatgat aggattcgat ttaggtggac cagtaaataa agcagcttgg
     3721 atggcaggaa atgcattatt gttagaggga atctatctac cagcgatatt ggtaaactgc
     3781 gcaatagtta tacctccact aggatatggt ttagctacat tcataagaaa atcaaaattt
     3841 tccaatgcat tagctgaaac aggaaaaggc aatatagtta tgggtataat tggaataaca
     3901 gaaggtgcaa ttcctttcac tttaacaaat ccattaaaat tagttcctgt taatatgata
     3961 ggatgcgctt tgggtgtagg attatcagct ttgttaggtg tacatgctat aatgcctcca
     4021 gttggtggat tatatggatt tatatcaata ggttctggat gggcttactt agtaggtgca
     4081 atatttggag ctttagtaat agcaatatta tcaacaactt tagtagactt caatgaagat
     4141 gaaactacgg aaaatacaga atttgatgaa atagagttag atataatgta aattaaaaac
     4201 ttaattttag ataattgaga attgagtgta ctctcaattc tcaattaaat tttgggagga
     4261 tttttatgaa aaagacaaaa gtacatgtag ttccacacag tcattgggat agagaatggt
     4321 attttacaac atcaaggtca aaaatatatt taatgaaaga ttttcaagat atattagaca
     4381 tattggaaga aaaagaagac tttaaatact ttactatgga tgcacaagct tctctcttag
     4441 atgattattt aaaatggaga cctcaggata aatatagaat aagagattta gttcaaagaa
     4501 gaagattagt tattggacct tggtatactc aaacagacca attggttata tcaggagaat
     4561 ctataattag aaacctttac tatggaatag atagatgcaa tgaatttggt gaacctatga
     4621 gggttggata tgtaccagat tcttttgggc aatcagctca aatgccacaa atatacagag
     4681 gttttggaat agatagttca ttattttggc gtggagtttc tgatgatatg gtagatacaa
     4741 cagagtttat atggaaaggt tctgatggtt caaaagtatt agcagttcaa ataccatttg
     4801 gttattacta tggaggtaat ataccagaag atgattcaga tttaaaagaa tatttaaaag
     4861 atataatcgt aaaacttaag gttaaagctt ctactaataa cgtatatttt ccaaatggat
     4921 ttgaccaagc accaataaga aaaaaccttc ctgatatatt aaaaaaagct aatgaaatag
     4981 ataatgaaaa tgaatacgaa ataagtgata tagaaaacta tatagattca gtaagaaatg
     5041 aaagaaatga atttacagag cttaaaggtg aatttttaaa tgctaaacat atgagaatac
     5101 acaaatccat attctcctca aggtcagacc ttaaaataat gaataacaaa ttagaaaatt
     5161 atattacaaa tgtacttgaa ccgttattaa cattaagtta ttcacttgga aatgaatacc
     5221 ctcacctagt tataaaagat atatggaaac ttatgtttga aaatgcagct catgacagca
     5281 taggttcttg taatagtgac acaacaaatg aggatgtata cacaagatac aaacaagcca
     5341 gagatttgag tataaattta ttagatttgc atatgagact tataagtact caaatcaaaa
     5401 ataataatga agaaataaca ttaacagtat tcaatggatt cccagaagtc agaaatgaag
     5461 ttgtagaatt tgatacttat attccaggtc aagattttgt gataaagaat aaaaatggaa
     5521 aaattcttaa ttatataata aaagaaaaag aagatattac aaattatata ttaactcaaa
     5581 caataagatt aaacccaagt aaaaaaatat atatacccaa aactgtatat agagcaaaaa
     5641 ttgcactttc agtaaaagat ataccaccaa tgggatatac tcaactagtt tttgaattgg
     5701 atactaaaaa taaatgtgaa atagaaacaa gtaattgcaa ggaaatagaa aacaaatact
     5761 acaaaataac agccaataaa aatggttcat tagaaatatt agataaagaa tctaataaga
     5821 cttataaaaa ccaagctgta attgaagaca atggagatga tggagattca tacaactatt
     5881 ctcctccaag aaaagatatt tatgtttcat ctttagattc tgtatcttca gtagaagtgc
     5941 taaaatcaaa cattcaagaa gaaatgatac ttaaatattc attgaccata ccagcttcac
     6001 tggaagaaag ggctataggt aaagtttctg tgaaaatgcc agtaacaatg aaaatcacct
     6061 tagaacctaa agaacaaata attaagttta atgttaatat aaaaaataac caagcactat
     6121 ctcatagagt aagagtttta tttaatacag aaatagcatc taagttttct atagcagatc
     6181 aacaatttgg aataatacaa agaccaacag tattagaaaa agaccttgca ttgtggaaaa
     6241 gagacaatta ctcatggcaa gaaaaaccta taactatcga accaatgcaa agttttgtta
     6301 ctttagagga tggacaaaga ggaatagcat taataactgg ttgtgtaaga gaatatcaaa
     6361 tagttggtga taatttagat acaatagcat taacactatt caggtcattt ggatatatgg
     6421 gaagagaaaa tttactttat agaccaggaa gagcttctgg agaaaaaata atagcaacac
     6481 cagatgcaca attacttaaa gagttaaact ttgactttgg tctttacata tataacaata
     6541 aatttgatga agcaaatgta gctaatacag ctaagagatt cttaacacct atacaaatat
     6601 atgaatatgc tgatttttta aatggtagac ttatatttgc attcaatgac gaaaaacaaa
     6661 tatatgaaaa tgaatttagt ttgatgaatg taaataattc aaatttcaca gtaagtgcaa
     6721 taaagaaaga agaaaaagga gatggaattg tagttagaat cttcaatggt atgaaaaatg
     6781 aggatgctaa gggaagtctt aacatcaata aaaatgtcaa tgatgcttat agtgcaatgc
     6841 tcgacgaaat ctatgataat actagcaagc ttaaagtaaa tactaaaact gtagaggtaa
     6901 attctttatc acattgtaaa gtacaaacaa tagtaattaa ataatataaa atagcttata
     6961 caagatttca aaataaattg ctagtaccat gttaatagta aagccagata ttatgctatt
     7021 tataagctct gtaacactaa ataacatttt gtaaaataaa aaggtgaacc ttgtatatta
     7081 ggattcacct tttttaaata aatcaacatt ctgttttaaa ataacattta taagctgttt
     7141 tgaaataatg cttacaaatt attttaaaac aatacctata aatttttatt taaagaatac
     7201 acataaatat ttccagactc ttttatccta tagactttat aattattaaa gaagtctaat
     7261 ctaatttttg ttaaaagtaa ttgtaacttt aaataaatca ccatccacac ttatattcat
     7321 cttaccaccc tgtaattcta taaaactttt tgctatcgct aaaccaagac cagaaccttc
     7381 tgtgttacgt gatttatctc ctcttacaaa tctctccatt atatcatcta cactaaaatc
     7441 aatctcagat gcagacatat ttttaagcat aatatttact ttattatctg tttctaaaat
     7501 atcaatatac actcttgaat ttggcattgc atatttgctt atatttatta taagattttc
     7561 aaatactcta aatgtacgct gactatctaa tggtaatatt attttttcat tagaaaagtt
     7621 attctttact attagagatg aatctgcaag tttgtcatct aattctaaaa gtgtctgctt
     7681 cattagagat acaatatcca catttactat attcaaataa acatctccac tatttgcctt
     7741 gcttacttca aacaaatctt ctattagatg ttgcaatctt tgtgactttc tgtctaatgt
     7801 atcaatatac aacttacgat tttcatctgt tatactttca tcttttaata aatctatata
     7861 agtgattata gatgttagag gtgtttttaa gtcatgtgat acatttgata taagctctgt
     7921 tttcatcttt tgactcttaa cctcttcgtc aacagctttt ttaaatccag tttgaatatt
     7981 tcctaattcg tcttttatag gattaaaaaa tcctaaatct ttttccatat ttacatctaa
     8041 gtttccatct gaaattttct ttgtaacatc taaaagttga ttatattcat atattgtctt
     8101 tttagagtaa tctgacagta ttttaaataa tactactgaa tatattaatg cgagtattat
     8161 tccaaagaac cacatactac acattataac tataatcaat aaatttattc ccaaaattgt
     8221 aactatagtt ttatttggct tttcttttag atttattgtt ctaatttgat taataactgt
     8281 ttttcctgct ttttttagcc atacaaaaaa cattcctgtc acagtatgtt ttgtaaaata
     8341 ctctcttatt ccaacattta aaatatgttt taaaagtatt ataaatacaa atattattga
     8401 gaatattaca aaccaataag ttaaattcat taaacttacc aatatactag ccattttagc
     8461 acttatgtca ttgtttataa ggtactctac ataattccca ctcaaggtat tacttattat
     8521 aacagtagga tataaatcta caagcccctg tataatagca agagcaataa agagtatctc
     8581 taacggtatt cttgataatg tatcaaatcc tagcaactct ttagattttc tatatgggaa
     8641 tgctaaagct acaataacaa ctatagctaa tataattttt aaaatcattc cagaaatctt
     8701 ataataagaa ttattttcta cattgcttat atatgaaatt ataccatcat caaaataagt
     8761 atttccataa ccatactcta cactatcacc tttacttatt actttaggta cagcatatac
     8821 taatgttata ttttttattg gagataactt atatgaaatt gaatttcctc ctaaacttgt
     8881 atccattaaa tcttctcttg ctttagataa caacatttga ttaataattt tttgatttac
     8941 tccataagaa tccacaatat ttacttttcc atctttatca aaattcatta caacgtagaa
     9001 tctatagttt tcttttatat ctgtaatctg cttttgtctt tcctcatcat tcatttctgt
     9061 attttttgac tgattttcat cagaattttc ttttgaagca tcattttttg ttgtatcaag
     9121 tactcctaat tctttattat cattaaaaaa ttcaaaattg ctttctttat caactaaata
     9181 gtactttaaa ttctttcctg acgctttaat gaaatgctcc caattttcaa agttttcatt
     9241 aaaatctgca ttaatactat catctgtata gtaagtttct gaaccatcat aagtatcttt
     9301 ttctttactt acatttttta tatagttcat agtttcttca ttgataaata gttttgatgg
     9361 ttgtatcatc ttaccttctt gttcttcctt catattatga tataaaacat aacttccttt
     9421 atatatacta tctacaaagc tttcctgttc aaatggattc tctgctccac ctttagcact
     9481 tgcttgtata gtagaatatt gactaatcat acctaatgat gctgataaca taagtataaa
     9541 aactataata gtaactatat atttatcttt aaattttttc aattttgtat ccaacacccc
     9601 acaccacctt taaatattta ggattttttg ggtcaatttc tatcttttcc cttatattcc
     9661 tcacatgtac cataactgta tctgtattta cagcattctc attccaaact ctttcatata
     9721 tttcatcagc agaaaaaact cttcctgcat ttctcataag taaacttagt attttaaatt
     9781 ctataggagt tgtttttata tactttccat caatactaac ttcttttgta ttttcattta
     9841 attctaatcc gcctattgca aacacactat cgtcactttc taccttttgc tctttatttc
     9901 caaccatgtt tagatactta gtgtaccttc taagttgtga attaactcta gccaatagtt
     9961 ctaatggctt aaaaggtttt gttacataat cgtctgctcc tatattaagc cccattattt
    10021 tgtcaacttc ctctgatttt gcagatagca ttattactgg aaaattatag ttttctctaa
    10081 gcttcattgt aagtgttata ccatccattt taggcatcat tatatcaact atagctaaat
    10141 gtatttcttg gttttctata accttaagac cttctaatcc attgtatccc ttaaaaacat
    10201 tatatccttg atttaataga tatatttcta tagcatctgc tatctccttt tcatcttcta
    10261 ccaccaaaat attatatgaa ttcattctaa tctcctttat attaatttat aaattttctt
    10321 tacatatatg attatacaat atgatagaag tttttccatt tatatatata tttatatctt
    10381 ttgttctggt tatttgtatt taatgtccat cttagaaacc tccttctagt ttaaatttaa
    10441 tattttttca ttttgtttta tatacttaat attaaaatta aaaacttaag atattacata
    10501 tacttttcta aagattttct aaagatttaa atatatttaa aattaatata caaaaaaagt
    10561 agttcttatt gtattaatta aatcaatgat ttgaaatata aaattaatat caataaaacc
    10621 tactttttaa ttttttaaat atttattttt aatttatttt gtttttaatt tacttcgatt
    10681 ttaaaatgtc cttgactttt attttataaa tgtacatatt tcttctactg gttttttaga
    10741 tggactcttc aaattgtcct ctggcactcc aagagctaac attgcaccta aaaaatactc
    10801 tcccttttca aaaccagttt ctgcttctaa aacagcttct atctcatctg ctgcatagtt
    10861 ttgacttgtc aaccaacaag aaccatatcc aagttcaatt gctgatagtg taaagttttc
    10921 aattgcagca cctagactct gcatacctgg atttcttata aataatttat ctatagtctc
    10981 cttaggtgca tctataagct ctaattcata atatcctgat ggattatata cctttgtaaa
    11041 aactaatact aaaactggag cttttaagta aaatagtgta aaatttttaa caaattttct
    11101 aaatctatta gccttatcca ctgatacttt atccatttct acaagtattt cttgttgctt
    11161 tttagttatc acatctgcta tcttttccat taaatcacgg cgttttataa caacaaaatg
    11221 ccagttttga atattcttgc ctgatggtgc tgcacctgct gcttttatca tttttaaaat
    11281 atcttcatct gatacatctt gatttttaaa ctttcttaca ctttgtcttt taaaaatagt
    11341 atcttgtaat tccattttta aatcccccca taactttttt attattttaa tataattttt
    11401 aaactcatct tattttaata tatcatgttc taacaaatct ttaaatgaat ctataaaata
    11461 atcatataca tctacattcc caaacccata tttggcatat ataaaaggta tacctacaaa
    11521 cttagcacta tttgcatcac cttgcgtatc accaacatag attggattta ttaatttatt
    11581 tctttcaatt ataagtttat tattttctcc tttatgcaaa ccagtattac caggacattc
    11641 aaaatctata aaatactgtt ctagtttatg tgcttttaaa aaagattcta tatatccagc
    11701 ttgacaatta cttactataa ataatttata ttttttagat agtaatttaa gagtatcttc
    11761 tatatcatca aataatttag ctccattttc agataaatag tcacattctt tcaaagaaca
    11821 ttcattcaat aaattcattc tttcttcttc acttaaattt ggaaataaac gatttgctat
    11881 ttcctcaaaa gaaagcccca taagacttct aaataaatct tttgtgacac ttaattctat
    11941 ttccttatgc tcacttaaaa catcctgcca gactttgcat acttcttcag ttgaatccca
    12001 caatgttcca tctaaatcaa aaataatact atctaccaca aaaaatcccc cttatgtcaa
    12061 agtttattat atatttataa ttatatacta aaaagcagat acaatcataa atatatttta
    12121 cttgattgta tctgctctac aatataaaac caattgtcaa aatcaaattt tataaatttc
    12181 taactacaag tacacagcct tgctctactt taatcttaca agaattactc aacataacat
    12241 ttgatattcc aataggtaca gagtatttca tataataatt atcgagaggg tattctaagc
    12301 cagtaagtgt tacaccctta gcatcaccat ttatagctat tacagaaata gtgtcccctg
    12361 ggtttccata caatgaaatt tcctcatttt caactatata catctcttct ttatcagaaa
    12421 gtatcctaga atatatacca tcttctttta aataatataa tagctttata tttgcaagag
    12481 tgtggtcaat ccttccccct aaagcaccaa aaaaatcaat atgatttgct ttaagtgtct
    12541 tagctaaaaa aagacaaagc tctgtatcag tctcatcttt tttagatgga aatttttcaa
    12601 atttaacacc tttattttta tagaagttta tcttttcttc ttctactgaa tctaaatctc
    12661 ctaatatata atctggcatt atttccattt tataagtgtg atttgcccct ccatctgcac
    12721 atattatgca gtcataacat tccttaatta taatatctct tgtaacttcg taatcttcaa
    12781 tttcgccatt taaaacaata cagattttca ttattaaatt taaactaatt cttcaagaga
    12841 agcattcttt ctaaataaat taactgtttc ttgaatgtca tcacttccaa atattgcaga
    12901 acctgctact attacattag ctcctgcttg tacaacttcg gctacgtttt tagggcttat
    12961 accaccatca acttctatat caatgtttaa tccttgacta tctataatag cttttaactc
    13021 tttaatctta tctaaaacac aaggaataaa tgattgtcct ccaaatccag ggttaacaga
    13081 cattaataat accatatcta aatcaggtag tacatgttta atcgtatcta ctggtgtagc
    13141 tggattaagt actacaccag cttttattcc atgtgatttt atattttgta tagttctatg
    13201 tagatgaaca caagcctcct ggtgaacagt tattatatca catcctgctt ttacaaattc
    13261 ttcaatatac tggtctggat tttctatcat aagatgagca tcaaatacca tatttatatt
    13321 ttctttcttt aaacttttga ctacaagtgg tcctaatgta atgtttggta caaagtgtcc
    13381 atccattaca tcgatatgaa gatattcaca tcctgcactt tctacttttc ttacatcttc
    13441 taataatttt gcaaaatctg ctgataatat tgatggtgct aatttaatca tgtttctaat
    13501 acctcctact attattttgt cttatttcat gtagcagctg tatataacta tcatatcttt
    13561 ctttagatat ttctccattt gtaacagctt cttttatagc acaattaggt tcattttcat
    13621 gaatacactt tgaaccaaac ttacaatcat taaatttatc aaattctata aaataatctt
    13681 taagttcaac ttcctctata tcttcaaggg caagggaact aaaacctggt gtatctgcaa
    13741 ccattccacc aaattctaat tttaaaagtt cagcgtgacg agtagtatgt tttccccttt
    13801 ttattttatc acttacaact cctgtttgaa gtttaaaatt ttcatctatt tcatttaata
    13861 agcttgattt tccaactcca gatggtcctg caaacactac tacattttct tttaattctt
    13921 cttttacttt atctatattt aacttagtaa tattgcttac aggaatgact ttatagccac
    13981 taagttcata tatattttta acagtttcta atgtatcatt atcatctaaa tctgcttttg
    14041 taaggattat tacagtttct aaattttctt tttctgccaa aactataaat ctatctaaaa
    14101 gagataagtt tggtttagga ttttttattg cgaatactat taatgcttta tctacatttg
    14161 caataggagg tcttataagt tctgtatctc tactgtcgat ttcttctaag ataccctttt
    14221 tttcattttc atcaacaatg cttattttga ctctatcacc aaccaaaggt gttattttct
    14281 gctttctaaa tatccctcta gccttacatt cataaatacc attataagta tctacataat
    14341 aaaatccact aatccccttt ataatttttc cttctagcat caaatgcctc cctttataaa
    14401 atttatataa acacatactg tttatgagta gtatatcgtg ttattctttc ctatttttat
    14461 ataataccac atttttttta aataacaaat cctccgtttg gactaattac ctgaccagtg
    14521 ataaaatttg atttgtctga tgccaagaaa atagcacaat ttgctatatc ttctggagtt
    14581 cctagcctca ttagaggggt ttcttccaca agagcgtcta tatcttcctc attatatcca
    14641 gataacatgt cagtattaat gacacctgga gctatgctat ttactctaat atttgatggt
    14701 cctacttctt tagctaatgc ctttgtcata cctattatgc ctgcttttgt tatagaataa
    14761 tgcacttcac aagacgcacc tgatataccc cagattgaag atatattaat tatatttcct
    14821 tttttttctg atatcatgta ttttaaagca acttgtgaac aataaaagct tccttttaaa
    14881 ttaatattca tcatattatc ccaatcttca tcagttatat ctgtaaacaa cttatcttga
    14941 cttacaccag cattgtttac aagtacatcc aatcctccaa attcttttat acaataatct
    15001 accatatcct ctacatcttc tctatttgaa atatcagcct taaaaagttt tacagaaaaa
    15061 tttttttcat ttaatatagt gtacaactct ttagcttcat tttctgactt attaaaattt
    15121 actaatacat tgtaaccttc ttttgcaaaa gctttagaca tagctttacc aatccctcta
    15181 gctcctcctg taattaaaac tgttttcttc ttcataaata cttttctcct tatattataa
    15241 ccaattcagt attttataca aaacaaactg ccctttaaag aaaatatttt ctataaaagg
    15301 acagctctta ttttattaat atttattctg ttttaccaat gtttgcgcca gttcctgatg
    15361 aagttcctcc attatctcct gagtttgagc ctcctgaact atctccattg tctcctgagt
    15421 ttgaacctcc tgaacttcct ccattgtctc ctgagtttga gcctcctgaa cttcctccat
    15481 tatctcctga gtttgagcct cctgagtttc caccactatt tcctgagttt gaacctcctg
    15541 agttacttcc attattgcct gagtttccac cactatttcc tgaatttgag cctcctgaat
    15601 ttccaccact gtttcctgag tttgaaccag gttctgttgg tgtattattc tctggtgttg
    15661 ttggtttttt atctggtact gttggagtct gtgtatttga tttttctgaa cctttgctta
    15721 catataggtt tacctcatca ccttcctgag ctcttgatga gccagcaccc ggactctgac
    15781 ttaaaactgt accttcttta tatattgagc tgtattcata cttaacagtt cccactttta
    15841 agttattttc ttcaataagt tgttttgcat tactcaaagt aagtcccact acattaggaa
    15901 cagatgcctg acttgaacct ttacttacga caacatttat agtatcaccc tttttgacat
    15961 ttgtacttcc accttcaggt gtctgtgata ttatacaatt ttcatcaact tcactattat
    16021 actcttcact ttttacactc aggtataatc cttctttttc aacagcactt tgagcttctt
    16081 ctaatgtcat atttacaaga ttaggaacag tcaaagattt attgccaaac cctccagcaa
    16141 ataaaaactt atatgctaag aatacttgag cacacaatat cagtatcaaa acagccgcaa
    16201 ttacttttaa tcttcttcta gattttgggc tttcctgttt tttcttcttt ttcttagttc
    16261 tcttacttgg agtactctta ggtctctgat tctttttagc tctcattatc tcttcctctt
    16321 cttcatcttc atcatcatct tcttcataaa aatcatcata gtaatcttca tcatcatcca
    16381 aatcagctac ttcaacaggc ttaaccactt tttcaggagc tggttttgca agtgttggat
    16441 ttacaacttt attaatttct ttttcatcaa ttttcttagt tgcaaaatca tcatattctt
    16501 ttataaaatc taaatcaata tttttttcta tatactctat atcttctatc aattcttcag
    16561 cagtttgata tctatctgca ctagattttt ctgtaagctt ttttattgta gttcttacac
    16621 tttgaggaat cctaactttt tcttctgatg taaagtctat atcatcatta atgtgttgaa
    16681 gtgctataga aattggacta tctcccctaa atggaacttt tcctatcaac atttcatata
    16741 aaactatacc tagagaatac aggtcagcat tattagttac aaatttacct ttagcttgct
    16801 ctggtgaaaa ataatgcact gaacctatta tactaccaat attagtcatt gttgaatttg
    16861 aaacagcttt ggctatacca aaatcagcca cttttactac tcttccttca tttgatatga
    16921 gaatattatg aggttttata tctctatgta taatcccctt tttgtgcgct gcactaagtg
    16981 ccatagctat ttgttttgtt atatctaatg cagtatattc atctaatata ccttcatctt
    17041 gtataatctc ttttagattc ttcccatcta caaactccat tacaatgtag tgaacttttc
    17101 catcttcacc aacatcataa acatttacta tatttggatg tgaaagactt gctactgctt
    17161 ctgcctctct tttaaacttt gttagaaatt catcatcatc tacaaattca ggtctaagta
    17221 ctttaagtgc aacagtcctg tttaataatc tgtctttagc ctcatataca aaggccattc
    17281 ctccatcacc aatcttcctg atgatttcat atcgatttcc taaaattgta tctcccacaa
    17341 tatcaccgtc ctaattatac ttttatcact ataaccgaaa cattatcttt accagaaaca
    17401 tcattggcca tacttatcaa ttcttcactt atattactag ttctctcata ttcataatct
    17461 aatattaaat ctcttatatc ttcatcatca ataaacccag ttaatccatc tgtcgcaaga
    17521 agtattatat cacttttttt gatttctttc ttaaaaatat ctacaacaac catatcatct
    17581 gttccaattg ctctagttat tctattcctc tgtggatgtc ttcttgcctc ttcttctgta
    17641 atgacatggg ccatcataag ttcttcaacc acggaatggt ctatagttat tttatcaaat
    17701 ttttcatctg ttaacaaata acatctgcta tctccaacat ttgccacata taagacatta
    17761 ttatacacta tagccgtaac taatgtagtt cccattcctt caaattctat atcttccata
    17821 gattttttgt gcacaattga gtttacatta ttataagcct gttttaatat atcatctata
    17881 tagtctattt ttacattatc atgttgtaat aagttttctt ttagaaaatc aattatattt
    17941 tctactgcta atttactggc gacttcacct tttttatgtc cacccattcc atcagctatg
    18001 gcaaatattc ctattggacc atgttctgta tctataactt ctccctcaca atagtcttca
    18061 ttgttttttc ttatttttcc tatatgggag gcacaactat aaaccatacg acttcctcct
    18121 tacaaattac ttgtattttc ttctcaattg cccacaagct ccactaatat cagaacccat
    18181 agacatccta actgtggcag gtatattgtt cttctccaaa ctatccctaa atttataaat
    18241 aaatgcttta tctggttttt cgtattctct ttcttcaact ttatttattg gaattaaatt
    18301 tacatgacat agcatacctt ttaggagctt agctaatgcc tttgcttctt tttctgagtc
    18361 atttactcct tttataagag aatattcaaa tgtaaccctt ctatttgtct tttttatata
    18421 atatctacat gcattcaata tttcttcaat agaataagca tttgctacag gcataatttt
    18481 tcttcgttct tcatcatatg gtgaatgaag tgataaagct aaatttatag ctatttctaa
    18541 gtctgccaac tcatacattt taggtactat tccacaagtt gaaagagtta tatgtctata
    18601 ccctatatta agaccatttt tttcgttaat tatttttaaa aattgttttg tattttcaaa
    18661 gttatcaagt ggctcaccac tccccatcaa tacaagattt gataccctct taccagtatc
    18721 ttcttgaatt ttaataactt ggtctaaaat ttcccatggc tctaagtttc taattaatcc
    18781 atccattgta gatgcacaaa aattacatcc catcctacaa cctacttggt tagaaacaca
    18841 gacagtaact ctactatcat aatccatcat cacagtttct attatatttc catcatctaa
    18901 taaaaacaaa tatttttttg tattatctac cttagattct aactttaatt ctatatctat
    18961 atgccctata catgaaacct cttctaattt atttctcaaa ctttttggta tattgttcat
    19021 atcatcaaaa gtttttgcac ccttatatat ccaagagaat atttgacttc ctctaaaggg
    19081 cttttcatct atagttttca taaactcttt aagttcatct tcagtaaaat tttttaatac
    19141 tatctttttc ttttccattc tctcactacc ttattctctt taattttgct atgaaaaaac
    19201 catctatacc atgaatatta ggatatatct ttaaatatcc tttatcttga ttgtctaagt
    19261 ctacatttac tgtatcaatt ggaacaaatt caaagttgtg attttcttca acaaaagaag
    19321 taactatatt tatattctcc acatcttgaa ttgtacatgt actatatact aaagttccac
    19381 caactttgac atatttagat gcattttcta aaattttctt ttgtatagaa gtgatgtctt
    19441 ttaactcttc ttctttctta tacttaattt ctggtttacg tcttattata ccaaaaccag
    19501 agcaaggaac atctgctaaa acataatcaa atttacttat actattttca tcaatctcag
    19561 aagcatcaaa tctttctaca aacacatttt taagccccaa tctatttaca gtagagttta
    19621 taagctttag tttatgctca aaaatatctc tagctactac ttgaccagta ttgttcatta
    19681 aagtagctaa gtgagtagat ttccctccag gagcactaca tacatccaaa acaaatgaac
    19741 cttcttttgg attaattact ttccctacta tcatagaact tatatcttga attgtaaata
    19801 aaccagcctt aaataattca tttttttcta tatttttcat tttttcgact ttaatagctt
    19861 cttctaccat aggaactttt aaacatacaa tacccatttc acttaatttt tcaataagtt
    19921 cttctctact tattttaagt gtattcgttc ttatatagat actaggcttt tcattatttg
    19981 cttccaacaa atcttcagca aattcttgtc caaatttatc tatccagttt tttataatcc
    20041 aagagttata ggaatacttt atagataagt attttacact atcttcctca gttatgtcca
    20101 ttatagtctc tttagctctt atttcatttc taagaatagc atttatgaaa ccagaagatt
    20161 ttttatcata tttcttagat aattttacag tttcattaac tgctgcataa tctgatatac
    20221 tatctaaaaa taaaatctga tatgttccca ttcttaaaaa gattttaaca taagttgaca
    20281 ttttttttac ctttattttt gaaagtttat tgattatata atctaaataa tatttatttt
    20341 ctattactcc ataaattaac tctgttgcaa gtcctctatc catatcactt atttctaaat
    20401 ttttaaaatg cttatttatt gcaatatttg aataattgtc atttttttct atatcacata
    20461 atactttaaa tccaacttct cttgcatcca tataaatttc tcccttttat taaacataaa
    20521 aaagggttgt ttctaaacaa cccactttac ttttagtcat ttcttctttg agctattagt
    20581 aacaatctaa gtaattgaag tattgaaacc aatgctgctg caacatatgt taaagctgct
    20641 gctgaaagta ctcttctact ttgtcttgac tcactttcat ctataatccc taaattccca
    20701 agttgaacga ttgctctact tgaagcatta aattctacag gcaatgttat tatttggaat
    20761 aacactgaag ccgaaaacaa tagtatacca atttttaaaa atggaccagc cataataaat
    20821 cctaaaaata ttaaaaacca agatatactt gaagcaaaat tcactactgg tactaaacta
    20881 cttcttattt gaagaggtgc ataaccttta gcatgttgta tggcatgacc acactcatgt
    20941 gcagctactg caacagaagt tatagatgtt ccataatata catcttctga caaccttaca
    21001 gcctttcttc ttgggtcata atggtcactt agatgccctc taaccatttc tatctttaca
    21061 tcatacaaac catttgaatc aagtactctt cttgctgttt gctctccagt atatcctcga
    21121 cgtgtattta ctcttaaata cttatttgtt gttgaactca ctttaaattg tgcatatata
    21181 gtaagtaata tagctggaat taatacaacc attgttgggt caaatcccca aaaaccgcca
    21241 tatacagggt acataaataa cactctccta aaatatattt ttattataca tcctcttaat
    21301 attcacttca cttaaaaaca gtttaattac aaactaatat attttttcct gttaaaaagc
    21361 ctatagcttt ttcaatatcc ataatgtcta cattagtatt tacacaaggt ccattaggtc
    21421 ttttgttaaa tacacctaaa acaggaattt tcttaatatc ttgtattcca gatgtcaaat
    21481 ctctttcaca tgcaacagct acaacagctt taggtttatt atccattata atttttctag
    21541 ctagggttcc tccagtagct acaaatattt ttacattagt tttttccttt agcttaatta
    21601 attctcctat gttgcatctt ccacattctt tacaattatc tatatcatta gtaactttta
    21661 atttacaact attctcttga atacaatgag gtattaaaat tattatatcc tctggattaa
    21721 aattatattt attactgtat atatattcat tatttaactt tatatatatt cttctaatat
    21781 cactttttga cataccaaat gatgatgcaa tgaaagatat aataggaaat aatccactga
    21841 caatattaaa atttattttc attatatttg agttaacata ctttccttta attactctat
    21901 aagtaactgc tgttgaacat agtattataa gtattagtga tattattata atatttatca
    21961 tcaaggagaa tatttgagtt aaacttatta taaaaaaatt aacaactcct atcaaaattc
    22021 ctaatattaa aatcaaaatc ccaacacttg ttaaatactt ctttatatct gtcatacttt
    22081 tcacctaacc caatactaaa ccagtttcta tagtattccc ttttatatat tcaccaacca
    22141 acattctctt tttattcggc atctgaattt cttcaattaa aagtacatta tctatagtac
    22201 ttacttttat cccatctttg cttacatcaa ttattgtacc tacatctttt gtgctttttt
    22261 catctaacac tttagttttc caaactttca ttatgacatc attataggta gtataggcac
    22321 ttggccatgg atttactcct ctaactaaat tgtgtatttc tctggcactt tttgaaaaat
    22381 ctatgttacc taatgattta ttcattattg gcgcatagga aaattcttca tgattttgta
    22441 cttctcttgg agcatttcct tcttcaatta atcttaatgt ttcttttaaa gtttctgcgc
    22501 ctatattcat cattttatca tgaagttctc ctgctgttat attttcatca agatttactt
    22561 ctgtctttag tatcatatct ccagtatcta gcccttcatc catatacata gttgtaacac
    22621 cagtcttttc ttcaccattt attattaccc aatttatagg tgccgcacct ctatattttg
    22681 gaagtaaaga aacatgaaca tttatacatc caagtttagg aatctctaat atttcttttg
    22741 gaagtatctg tccaaaagct acaactacta ttacatctgg atttaaagat ttaattttat
    22801 ctataaattc tttatctcta gcctttactg gttgatatac aggtatatta ttttcaattg
    22861 ccagttcttt tactggactc atacctagtt ttttacctct tccttttggt ttatctggtt
    22921 gagttacaac acccaatatt tcatacttct catctattat tttttgtaaa catggaactg
    22981 ctatatcagg agttcccata aatactattt tcatcaaatc aactcctact acttttctat
    23041 tttgtcaaca aacaaaattc catctaagtg gtctatctca tgacaaaatg ctctagctaa
    23101 aagttcttct ccctctaact ctattgtttc accatttcta tttaaagctc tgacctttac
    23161 ataatttggt cttctaacat ttcccgcttc tccaacaaca cttaaacatc cttcctcatc
    23221 tatttgctcc ccagatgttt ctattatttc aggatttata agttctataa gctcttctcc
    23281 aatatctatt actactactc tttttaatat tccaacttga ggtgcagcca gtccaacacc
    23341 atctgcatca tacatagttt ctgccatatc atctaataat tgtattatct tttcatcaat
    23401 tttttcaacc ttttttgatt tttttcttaa tactggttct cctatttgaa ctatttgtct
    23461 taaagccatt ataattcctc ctaaatttat aatacactgt ttggattaat atctatagat
    23521 acattgatat tcttatcaaa tatcaaatct cgttttgtta tacatatata ttttattata
    23581 ccctttaaga gattaatttc aatattttca tctttaaaga gtatttgcca tctataattt
    23641 tgatttattt ttgatattga acatggattt ggacctaata taaatcctaa atcatttata
    23701 cccctttttt ctaataaata aatcaatgat gcatgcatat tttttatatt ttttatcact
    23761 agtctttcat cttcaccact aacaactaca cttaacatat tattaaaagg agaatatcca
    23821 aatgcttttc ttatttttat ctcatcttca taaaaaccct cataatcata ttcaatagca
    23881 tgcttaatag catagtgttc agtgtcataa gtctgaagta ctacttttcc ctctttatca
    23941 gctctccctg ctcttccaga gacttgagtt atcaattgaa atgtagtttc aaaactttta
    24001 aagtctggaa aatttaatat catgtctgca gacaaaatac ctactaaagt tacattctca
    24061 aaatccagcc ctttacttaa catctgtgtt ccaataagta catctgcttc tttatcttta
    24121 aatttattta gtatctcatc taaagcacct ttttttgaag ttgtatcttt atccattctc
    24181 aaagttttta tatctggaaa aatatatttt aattcctctt caattttttg agttccaact
    24241 ccaaatggct taacataagt acttttacat tcaggacact cttttggaat ttctttctca
    24301 tatccacaat agtggcatct tccagtattg cttttcttat gataagttaa agatatatca
    24361 caattttcac attgaaacac atatccacac tttctacaag atacaaaatt tgcataacct
    24421 cttctgttta aaaatagtat tacttgatta ttttcttcaa tcgcataacg tatttctttt
    24481 tgtaatttca aactaaatat acttctattt cctttatcta actcatcttt catgtctacc
    24541 acttcaatgt ttggaagagg cttatcattt gctctacttt ttatatttat aagtttgtat
    24601 tctccagatt ttgccctata atactcttca attgagggcg ttgctgaacc taaaataaga
    24661 gttatattat tttttaatgc cataaatcta gcaacttcta tagcattaaa ttttggattc
    24721 ttctcagact tatatgatga ctcatgacat tcatcaatta ttattaagcc taaggaatta
    24781 aaaggtgcaa ataaagctga cctagctcca attaacactc ttactttacc tgctttgaca
    24841 gctttgtaaa catcatgttt ttctccttca gatagtttac tgtggaacac tccaaccaaa
    24901 tctccaaacc tactctttag cctatctatt gtttgaggag ttaatgctat ctcaggtact
    24961 aagaatatac tgtctaagcc ttgatttaga gcaaattcta ttatttccat gtaaacttca
    25021 gtttttccac ttccagttac tccatgaatt aagtatggtt ttttatcatc tacaaacata
    25081 ttcgacttta tctcatcaac tgcttcttgt tgctcattgt ttaattttat actttttagt
    25141 acggatttgt aaatactttt tggctctcta taataatcct taaattctag ggttattagc
    25201 tttttcttac ttagagaatt tatactttgt tttgaagcat ttaataaatc caataaatca
    25261 tttatctcta cattttcatt atttttaaga aaacttaata tctctttttg tttacttccc
    25321 acatttattt tattttgctc tatataatcg tcaatcttat cactttctaa actcaaagat
    25381 atataacata ccttcttttc atttttgtga tttttatatt cccaacataa ttttataatt
    25441 ccattcttat tcattgtgta caaagaacta tttaagtttg gtatatattt taacttatca
    25501 accttaattt tacctttact atcaatcact ttctctacta tttctctatt tttatcactt
    25561 aaagttgata ttttttcctt caattcaata tcatttaatc cacttaaact ttcacctaat
    25621 aggactactt tataattatt taatttgtat ccctttggat atatcaaatt tatacattct
    25681 atataagtac acaaatatct atttttcatc caataaacca actctaaatc ttcttttcta
    25741 aatatcggat tttcatctaa tatatcaata atttctttta tttttatttt ttcatctaag
    25801 ctatctgtaa attgaaatac aaaggcttct gttggtttat taccttttcc aaatggtact
    25861 aatattctgt gcccaatttt tattacatca gataaaaata caggaatttg atatgtaaat
    25921 aaattatctg tatatatagt attacttcta actattacct ttgcatattt tttcattata
    25981 aaacctctac ttaaatttaa tatattgttc atattttaat atactttaca agatattaaa
    26041 atctcagaat ttataagcaa tttcaagtta ttaatattaa ctttatttta ataattaact
    26101 attaattata ttttatactt tatgacaaaa taaatgctga aaactacaat ttaactgaca
    26161 tttgcgagtt tttactcgct aaaattaaat tgcagctttc agccacttta gctgtgataa
    26221 aatacacaaa tatcatattt aaaaaaggtc ttatttctca agcaacgctt taactttatc
    26281 taaaataaca ttggctactt cttctttttt catcttagga tactcagtaa tatttccctc
    26341 tacatctatt atttttacaa tattagtatc agctctaaaa ccagcacctt ctttagttaa
    26401 atcatttgcc acaataaaat ctaaattttt cttttttatt tttcctttgg catgttctat
    26461 aaggtcatta gtttcagctg caaatccaat tagtatctta tcttctttta tttttcctat
    26521 ttcataagca atatcataat ttctacttag ttctattgat aaatcatcat tcttcttttt
    26581 aatttttttg tcagaataag tttttggttt ataatctgca actgctgcac tttgaattac
    26641 tattttattt tcacttaaat ttgatactac tgcttcatac aaatcttgtg ctgactctat
    26701 ttgaacaaat tttttaagat tttgtggaat agaaagattt gtaggacctg atattaatgt
    26761 tacatcagca cctctagcta tagcttctct tgctatagaa tatcccattt taccagaaga
    26821 cctattagtt aaatatctta ctgggtctat actctctaca gttggaccag ctgtaactat
    26881 catactttga ccttttaaat cttgctcttt agtaagatta gtcaaaactt cctctactat
    26941 tatatctgga ttagctagtt tacctgttcc aatatctcca catgcaagcc ttccactttc
    27001 cggttcaata aatttatatc caaagctctt taaagtttgt atattttttt gaacaattgg
    27061 attttcatac atattagtat tcatagctgg tgctataaga acctgtgctt ttgttgccat
    27121 aacagtagtt gtcaacatat cgtcagctat accatttgca atcttgccta taacatttgc
    27181 acttgctggc actattaaga ataaatctgc tttttttgct aatgatatat gttcaacttc
    27241 ccaagttttt ggttcttcaa acatatcttc tactacataa ttttgactca gcgattgaaa
    27301 tgtaagtggt ttaacaaatt ctgttgctga ttttgtcatt ataacatgga tatttgcatt
    27361 tagttttttt aacttactta caacatcaca agccttatat gcagctatac cgccacttat
    27421 gccaattaca acagttttat cttttagcat gaccttactc ctcttctgaa atttgttggt
    27481 gttgctcagc atgatgtcta gcttcttcta tctctatttc ctcttctgtc aataatctat
    27541 atgttatttt tccacttgca acttctttag ttgctacaca tacaggcttt tccttagttt
    27601 tatttgaaac atatggttct tctccatcaa ttaattttct tgctctcttt gaaacagtcc
    27661 ctactaaata gtatctgttg tctatttttt ctagtacctc attgatagat ggttttaaca
    27721 taattataat tcctccttaa atttctctat tataatattt ttatatctgc taactttatt
    27781 cttttcagat gatattatac cttctatttc tttagcagat gtttttacat cttcattaaa
    27841 tataaaataa tcataatcct taatcatttt aatttcttca tatgcacagc taaatctctt
    27901 ctctatttca gcttctgttt ctgtacctct tccaacaatt ctatttttta attctttaag
    27961 tgatggtggt agtacaaata taaatactcc ctctgggcaa acttctttta tttgttttgc
    28021 tccttgcatt tcaatctcca gtaatacatc ttgtcctttt tctaaacatt ccattattgc
    28081 cgcttttgga gttccataaa aattatcata aatttgagca tactccaaaa actcaccttt
    28141 ttctatcatc tcttcaaact tttccttact tatgaagaaa taatttactc catcaacttc
    28201 tccagttctg ggttttcttg tagtagctga tacagaaagt tttattgtat cattttcttt
    28261 aagtagttct ttgcaaatag taccttttcc tgcacctgat ggtcctgata caactagcaa
    28321 taatcctttt ttcacaaaca tttaagcatt ccccttctaa tattgtattc ttactctata
    28381 ttttgtattt gttctcttat tttttccaat tcgcttttta cttctactac taaattagtt
    28441 atatttatat cagatgactt cgaacctata gtgttagttt ctctattcat ctcttgtatt
    28501 aagaaatcta tttttctacc tatagagtcg tctttaaata tagtattttt aagttgccct
    28561 atatgacttt taaatctaac tatttcttct gttatactgc ttttatctgc atatattgca
    28621 acttcttgtg ctaatctatt ctcatctatt atacttgggt catccagtaa ttcacttatt
    28681 ctcaaattca atttttctct ataatcttct acaacactac ttgaatattt ttcaatctct
    28741 tctatatgat tttttaataa atcacatctc atggcaatat cttcagctaa ttttttacct
    28801 tcttcacttc tcatttctct taatttgatt aatgcatctt caactgcttg attcaacatt
    28861 gaccacaata aatcttcatc ttcttccttt tcttctattt tcacaatatc aggaaacttt
    28921 gcaacattca taacgctaat atcatctata atatcaaatt tattttttat ttcttttagt
    28981 atgtctatat actgagatgc taatccctca tcaaaattta agtttacatc ttctttgcct
    29041 aataagtcaa actttatgta taaatctact cttcctcttt ttacataatt ttttactaaa
    29101 tttctaactt tatcctctaa aaatgaaatt tttcttggaa gacgtatgtt tatatctgaa
    29161 tacttatgat ttatcgtttt acactctact aaaaagtaat aattatcatc cttgtattct
    29221 cctcttccaa aaccagtcat acttatagcc attaattaaa cctccaaaat accttcacat
    29281 atttttactg ctggacctgt catataagag aaatctccta catctatttt aactgttcca
    29341 ccttgagacg ttacatttac actactttct accatattaa gtaaattgca cactattgct
    29401 gatgcagtca taccagtacc acaacctaaa gtatatccac aacctctttc ccaagtataa
    29461 acatgtatat tatacttatc ttctaccttt acaaaattta cattcgtttt ttcaggaaat
    29521 attttattat tttcaatctc ttttccatat ttacaaacat catctacatc taattcatct
    29581 actataacta ctgtatgagg aacccccatc aacatagaag taactttaag ctccctatct
    29641 aaaaccttta tactttcttg cacaaaacgt tctttatttg taagtacagg aacgtcctta
    29701 gctataaaac ttccttttcc catattgact cttatagagt ttatttctct attagtaaga
    29761 tttactttta tatcttttat accatctaat gtatctactg taaaactttc ttttttaact
    29821 atacaattat cataaacaaa tttacaaaaa catctaaggc catttccaca catagctgct
    29881 cttgaaccat cagaattata atataccatt tctacatctg caacttttga atctttcaca
    29941 actaacaatc catccgctcc tacagaaaat cttctatggc atactttttt tgctaaagca
    30001 gtataatcag atggatttat ctggtcgaat cttccatcta ttgctataaa atcatttcct
    30061 attccatgta gtttccaaaa tttcataagt atcgctcctt cgtaaattaa cacttaaact
    30121 attataacat aaaaaaattc ccataaactt ataatttatg ggaaaagttt tgtaatgaca
    30181 actataattt gaggatgtga gggagagttg ccattacaat tagggtaatt atattatgtt
    30241 tttaagctag ttctttctat gtttatatat taatatttct atatgaattt tatgtatgtt
    30301 ttatttaaac tgtctatgaa ttttttaatt aaacatctgg taactagcta taatccttct
    30361 agtagatatc tattaacata cacctattaa ttctttaagt gtttttaaaa catagtctaa
    30421 atcttcctta gtaatatcta catgagttac aaatctatat tcaccatttt ccattttatt
    30481 aatttttata tttctttcaa aaaattcttt tattaaaaca tcctctttta taatatcttc
    30541 actcatctta aaaaatacca tacttatatc tggattaaca ttgtttactt caatgccatc
    30601 aattttattt aattcacaag ctaaatattt tgcattttca tggtcttcat tcaatcttaa
    30661 agtcattttt tcaatggcaa ttataccagc tgctgcaact atacctactt gtctcattcc
    30721 tccacccata actttttgct ttcttctagc ttttgctata aattctttat ttccagcaag
    30781 tatagaaccc attggtgcag ctaaaccttt tgacacacag aacataacac tatcacaaca
    30841 ctttgctatt tctttagctt ctacacccaa agttaatgct gcgttaaata atcttgctcc
    30901 atctaaatgt actggtaagt tatgtttctc agctattttt ttaatttctc ttaaattatc
    30961 aagactcaca actgttccac ctgaatatgc attttctaca catataagcc ctgtctgtgg
    31021 ttcatgtatg tcatctcttc taattgcctt ttcaacaagt tcaggattta gagctccact
    31081 ttcgtcttca tctatagttc taagctgaac tccagctatt acagcaggtg ccccaacctc
    31141 atgagtcaca atgtgataac ctttaccaat aattatttct tgccctctta ggcaatgagt
    31201 aaatagagct aattgattcc caaaagttcc acttggaagg aaaatagcat cttcttttcc
    31261 aactttagct gctgctaatt cttcaagttt atttacagta gggtcatctc taaagattga
    31321 atctccaaca ggagcctttg ccatagcttc tctcatttcg tcagtaggca tagtaacagt
    31381 atcacttctt aaatcgataa atctcatttt ttcaaccttc tttcatattt atatactata
    31441 gaaccttaaa attgattttt tctgtaaagt tcatatactt tcatagctta tactctacac
    31501 aataattata ctatatttac tttatattta atctacttta aattattaat tttaaaaata
    31561 aagtttcctc tctaataaat ataaataatt atacaattta aatattaatc tctattgttt
    31621 aaattccccc tagtcacaaa gcgtatatta tgtgtataat taatttactg actcattttg
    31681 gtcaaagaaa ggggtgctat aaatggcatt agatggatta gttatacatt caatagtcga
    31741 tgaattatca tcaaaactta caggtggaaa aatagataag atacatcaac ctgaagatga
    31801 tgaggtaatt tttaatataa gaaataataa agaaaatttt agattggttt taagtgcaag
    31861 tgcctcaaat cctagagttt atcttacaag taattatcaa aaagaaaacc ctctaaaagc
    31921 gcctatgttt tgtatgttac ttagaaaata catacaaggt ggaaatatag tagaaatttc
    31981 tcaaataggt tttgaaagaa taataaaaat aagtgttgaa tctttagatg aattaaaaga
    32041 aaagacagtt aaaaatatta tgattgaaat tatgggaaga catagcaaca taataataac
    32101 tcatggtgaa gaaaataaaa ttatagattc tataaaaaga gtccctttta gcataagtag
    32161 agttcgtcaa gttttacctg gtcatgatta ttcattgcca ccagaacaaa acaaactcaa
    32221 ccctcttgat gatatatcta aggatttgtt tattaaaaac ctagaggaat tggaaggacc
    32281 tatctttaag tctatatatt ctaaatttct aggtataagc cctattatag ctaaagaaat
    32341 ttgctacaga gcaggtgtaa atcaaaatgc tatcataaag gatatatctg atgaacaatt
    32401 tgattcttta cacaaagtat tttgtaattt attcaatgat ataaattcta acaaatactc
    32461 tccttgcatt attatagata aaaaggtgga taaggtagtt gatttcagtt gtataaacct
    32521 tacattattt agcgatttat catatataaa taaggatagt atgtctcgta ttctggaaga
    32581 tttctataga actaaggata ttaaagatag aattaatcaa cgttcatctg atttaaagaa
    32641 gagtatatct gttaagcttg atagacttta taataaactt aaaaaacaag aagaagaatt
    32701 atcagaatcc gaaaatgcag acatttataa gataaaagga gaattgatta cttcatatat
    32761 ttatatggta gaaaaaggta tggaaagtat tgaagttgct aacttctatg atgaaaattg
    32821 taatgatgta acaatagaat taaataaaaa cttgacacca tctgaaaatg ctcaaaaata
    32881 ctttaaaaag tataacaaaa tgaaacatgc taaagtcgag atatctcatc aaatttcctt
    32941 aaataaggaa gaaatagatt acttagaaaa cataatttta agtattgaaa actgtgaaaa
    33001 tttagctgaa ttacaagata taaaagaaga acttgctaag gttggataca tcaagactca
    33061 aaagaaaaac agtaaaaagg atactatccc atctacaaaa cctcatgaat ttgtttcttc
    33121 tgatggattt aaaatattgg ttggtaaaaa caataagcaa aatgactatc ttacactaag
    33181 acttgctgat aatgatgact tatggatgca tacaaagaat attcctggtt ctcatgtaat
    33241 aataaagtgt gctggtaagg aagtccctga taatactgta tttgaaggag ctatgctagc
    33301 agcattcttt agtaagtcaa aactttcttc tcaggtgcct gtagactata ctaagagaaa
    33361 aaatgtgaaa aaacctagtg gttctaaacc aggtatggtt atttatgaaa ctaacagtac
    33421 tatttatgtt actcctgaag aagaaacagt agccaagctt aaggttaaat ctgaataaat
    33481 tctgcactaa atacaaaata aatgttagac aactacacat tatcattcta aataaaaata
    33541 gtggccttct tataaagtac aataataagg ccactttcta attagcttac ttttaatttt
    33601 ctaacaaaat acttttctgt tttaataact atacttatat tcccaacaaa tattttcagt
    33661 cttcttatat agatgtaatt aaaaagttaa cactaaaagc tattttttaa tttattgatt
    33721 tgttttagta attcattata ttctgattca tacaattctt tttttagatt atctttttct
    33781 atagataaca tagatataac agttgtaagt ctattttcta atactagaag attttctttt
    33841 ttgatttggt tttctctact aggagttttt ttatttctgc taattgtata ttcgtcataa
    33901 ttacaatcaa actcccttat tttagcatct tttatctcta taatatagtt acaaatatgt
    33961 gatacaaata ctctatcatg agacactata agcatagttt tatcagtatt tatcagtgcc
    34021 ttttccagtg cttccataga ttttatatct aagtaatttg ttggttcatc aaatactaaa
    34081 aaattattat cttccaatat tattttacat agtgctattt ttaccttttc tccaccacta
    34141 agtactttta cttttttgta gacatcatca cctttaaacc caaataggct taagtttatc
    34201 cttataaaag attcatcaaa tgaactatta actttagtat tgtatagaac actcttttcc
    34261 tcatctaata aactttgatt ttggtcaaaa taacctactt ttaccttgtt atttattttt
    34321 atattgtcat ttttatcagc caatatttct tttatcagag tactttttcc acacccatta
    34381 tcacctaata atgctatctt tttacctctt tttatcttaa aggaaacatt atctaacagg
    34441 agcttatttt ctaactttag agtcatatct tttacctcta ctagattttt actaattatc
    34501 tccataccat cttgaatctt aatgttcatt tctttagaaa ctttaggttt attctttacc
    34561 tcaagtttat caattctgct ttttatagcc ttcacatttg agtccaactt tttcttatta
    34621 gtctgaccac ccattttgtg caacctagct tctgaattac ccattctttt tggtgttttt
    34681 cttatgccat cacttaaagc ttttttatat aaaagagctt tttcaagcct ttttttctct
    34741 ttaacatatt cttgatactc aaaatcagct ctttttactt cttcatcttt ttgcattaaa
    34801 taatcagtgt aattgccttt gtaagctttt agttttccat cttctagttc taatatagtt
    34861 gtacataact catccagaaa acgtctatca tgagatatta ataataatgc tccctcatgc
    34921 cttttaaaca tatcttccaa aaccccaatg ctttttttat ctaagtttga agttggctca
    34981 tcagctatta tgatattttt cttattactc agcgcatctg ctatttttaa cttaaccttt
    35041 tcccctcctg ataagtgttc ttcaaattta tctggagcat ttaaaagact cttctcctta
    35101 ctatgactac atgtttcaat attattttca ctctgactta tataagaata gctttctgta
    35161 aggtaagtat atccttcatc aatttctatt tctcccaata ttgcttttaa taatgttgtt
    35221 tttccttgac catttgaacc aactagacct attttgtcat tttctaatat ttcaagttta
    35281 tctatatcca aaattaattt atctgcataa tatttcttta aattctctac ttttactaac
    35341 aacataaaaa atccctcctt aacacactta aatataagga gagattctat aaataggcat
    35401 aacaaataaa cgtatagact ataatctact ctccttaatt taagcattgc aaataaagcc
    35461 accaaaatag gcttttcaaa aattacaata ctttaaatta taaaaaatag attatattct
    35521 cattgtccta gttaccttgc cctttctttc atttattttt aatcattata tcaacattaa
    35581 ttacaaatgt ctatagaaaa ttaactaatt ttagttattc aatatacaac ttattcttta
    35641 aatttattct tcttcaactt taaagtcctg attatttaaa attctgttta atacaattcc
    35701 aacaattgca gctaaactaa gaccagttat ctttactgta cttgttatag ggattccaac
    35761 agaaatacct ttattagata aatatgttgt tccaagacct attaataata taactgctat
    35821 gattaatata tttattttac tttctacaca agaactgtcc cttatagttt ttaatcctac
    35881 ataagttatc atcgaaaata gcattatact aattccaccc ataactgaac ctggtatact
    35941 ttgaagaaat ccaccaaact tgcctacaca tgcaagcaat atagcaaata ttgctgtacg
    36001 tctaagtatt gaagggtcat agtttttagt tatagcaagt actgctgtat tttctccata
    36061 agttgtattt gctggtcctc ctaaacaccc tgcaactata gtcgcaaatc catccccaag
    36121 taatgttcta tttaatccag gattttctat aaagttttta cctactacag caccattagt
    36181 agtcatatct cctatatgtt ccataaatac agctaataca actggagata taatcactat
    36241 tgctcctaaa ctgaacttag gaactgtaaa gttaggtatt gcaaataaac ttgcttctgt
    36301 aatagtagtt atatctacta atcttaatat caaacagatt atatacccag aaaaaactgc
    36361 tattaatatt cctaattgtt ttataaatcc tctaccaaac ttattggtta aaattgctat
    36421 tgctaaagtt ataaatgcta tcacaaaatt ggctgaagcc atactaaaag ctgttggcaa
    36481 taaatttaat cctataacta ctatcatagc tcctgtaact tgtgcaggaa aatataattt
    36541 aattttattt acaccaacta cttttactat aatcgacatt ataatataaa ttaatcctgc
    36601 tactattata ccaccttgag catatgctaa atctccacca tacgcctctt tagctgctag
    36661 tataactggt ataaacgcaa aacttgaacc taaaaaggca ggtacttttc cttctgtaca
    36721 aaaatgaaat atcaatgtac caacaccagc acagaaaatt gcaactgacg gattaaaacc
    36781 tgtcaaaatg ggaactaata ctgtcgctcc aaacatggct aatagatgtt gtaaagacat
    36841 tatcgttttt ttcataaaaa cctccaaaat tattatatat aaataaatcc tctgtttaat
    36901 taataccttt taattaatta tcattcccta gattctattt tttaacctct atatacttat
    36961 tatataattt atcattattt tacattttac gacatcaaaa actcaaataa aggagaataa
    37021 aaactacatt aagactcaca tccataaaat tataatattt attttgtgca atctatgtaa
    37081 gctttatcct cctccctgac tattcaatgc taagctttta tataatactt aaaaataatt
    37141 attctttgat tgtaactgaa tcttctccat caatttctaa taaactgaca gatataattt
    37201 catgtctaga agtaggtaca ttttttccta cataatctgc tcttataggc aactctctat
    37261 gccctctgtc cactagcact gctaattgta ttgatttagg tcttcctata tctataatag
    37321 catctagtga agctctcaca gttcttccag tgtataaaac atcatcaact aaaactactg
    37381 tcttatcatt tacattaaaa tctaaataag ttcctttaac tacaggttca acatgaattt
    37441 tttccaaatc atctctatac aaagttatat caatatctcc tgtatctact tttgttcctt
    37501 ctatttgttc tattttctta gaaattctat ttgcaattgg cacacctcta gtctttattc
    37561 caactaaaac tagattttct acacctttat ttctttcaat tatttcatga cttattctag
    37621 taattgctct tgctattgcc ttttcatcca ttaattgagc tttttctacc atatcataat
    37681 tattgtcaaa gatataatat atctatttga caaatctact cctttctttt aattttttat
    37741 aaatttattt ttatttaaaa tttcaattta aacaacaaaa aaacttctca tcacaagaca
    37801 agaagctctt atataaaaaa tatacagtgt aatttagcaa ttgtcttacg atatctctgt
    37861 atcgtttaaa gataattttt taatctatca actcaaatta tatatttata tttcaaattt
    37921 ttcaagcttt tttaaacaaa atactttaat ataagatatt ttatttaaat ttcactaaat
    37981 ttatttactt atataaaaac
//
LOCUS       DAELTA010000016        36984 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.016, whole genome shotgun sequence.
ACCESSION   DAELTA010000016 DAELTA010000000
VERSION     DAELTA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.016"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(258..401)
                     /gene="scfA"
                     /locus_tag="KPG13_000722"
     CDS             complement(258..401)
                     /gene="scfA"
                     /locus_tag="KPG13_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG0984466.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(467..829)
                     /locus_tag="KPG13_000723"
     CDS             complement(467..829)
                     /locus_tag="KPG13_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG0984467.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(887..1180)
                     /gene="yajC"
                     /locus_tag="KPG13_000724"
     CDS             complement(887..1180)
                     /gene="yajC"
                     /locus_tag="KPG13_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG0984468.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1314..2435)
                     /gene="tgt"
                     /locus_tag="KPG13_000725"
     CDS             complement(1314..2435)
                     /gene="tgt"
                     /locus_tag="KPG13_000725"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG0984469.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2514..2999)
                     /locus_tag="KPG13_000726"
     CDS             complement(2514..2999)
                     /locus_tag="KPG13_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984470.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3011..4036)
                     /gene="queA"
                     /locus_tag="KPG13_000727"
     CDS             complement(3011..4036)
                     /gene="queA"
                     /locus_tag="KPG13_000727"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG0984471.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4354..5373)
                     /gene="ruvB"
                     /locus_tag="KPG13_000728"
     CDS             complement(4354..5373)
                     /gene="ruvB"
                     /locus_tag="KPG13_000728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG0984472.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5480..6082)
                     /gene="ruvA"
                     /locus_tag="KPG13_000729"
     CDS             complement(5480..6082)
                     /gene="ruvA"
                     /locus_tag="KPG13_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG0984473.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6075..6584)
                     /gene="ruvC"
                     /locus_tag="KPG13_000730"
     CDS             complement(6075..6584)
                     /gene="ruvC"
                     /locus_tag="KPG13_000730"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG0984474.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6896..7525)
                     /locus_tag="KPG13_000731"
     CDS             complement(6896..7525)
                     /locus_tag="KPG13_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984475.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7728..8051
                     /locus_tag="KPG13_000732"
     CDS             7728..8051
                     /locus_tag="KPG13_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0984476.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8319..9173
                     /locus_tag="KPG13_000733"
     CDS             8319..9173
                     /locus_tag="KPG13_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984477.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9359..10795)
                     /locus_tag="KPG13_000734"
     CDS             complement(9359..10795)
                     /locus_tag="KPG13_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG0984478.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10866..12248)
                     /locus_tag="KPG13_000735"
     CDS             complement(10866..12248)
                     /locus_tag="KPG13_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0984479.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12384..13289)
                     /gene="garR"
                     /locus_tag="KPG13_000736"
     CDS             complement(12384..13289)
                     /gene="garR"
                     /locus_tag="KPG13_000736"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG0984480.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            13685..14356
                     /locus_tag="KPG13_000737"
     CDS             13685..14356
                     /locus_tag="KPG13_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984481.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14459..15007
                     /locus_tag="KPG13_000738"
     CDS             14459..15007
                     /locus_tag="KPG13_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984482.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            15269..15577
                     /locus_tag="KPG13_000739"
     CDS             15269..15577
                     /locus_tag="KPG13_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984483.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15739..17529)
                     /locus_tag="KPG13_000740"
     CDS             complement(15739..17529)
                     /locus_tag="KPG13_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984484.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17529..19256)
                     /locus_tag="KPG13_000741"
     CDS             complement(17529..19256)
                     /locus_tag="KPG13_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984485.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19501..19767)
                     /locus_tag="KPG13_000742"
     CDS             complement(19501..19767)
                     /locus_tag="KPG13_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG0984486.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            20065..21177
                     /locus_tag="KPG13_000743"
     CDS             20065..21177
                     /locus_tag="KPG13_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0984487.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(21541..22050)
                     /locus_tag="KPG13_000744"
     CDS             complement(21541..22050)
                     /locus_tag="KPG13_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0984488.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(22052..22699)
                     /locus_tag="KPG13_000745"
     CDS             complement(22052..22699)
                     /locus_tag="KPG13_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984489.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(22713..23885)
                     /locus_tag="KPG13_000746"
     CDS             complement(22713..23885)
                     /locus_tag="KPG13_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0984490.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            24153..25502
                     /locus_tag="KPG13_000747"
     CDS             24153..25502
                     /locus_tag="KPG13_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984491.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKYIKLISYLF"
     gene            complement(26464..26994)
                     /locus_tag="KPG13_000748"
     CDS             complement(26464..26994)
                     /locus_tag="KPG13_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0984492.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(27082..28176)
                     /locus_tag="KPG13_000749"
     CDS             complement(27082..28176)
                     /locus_tag="KPG13_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG0984493.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(28178..28624)
                     /locus_tag="KPG13_000750"
     CDS             complement(28178..28624)
                     /locus_tag="KPG13_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0984494.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            28930..30120
                     /locus_tag="KPG13_000751"
     CDS             28930..30120
                     /locus_tag="KPG13_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0984495.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            30290..31252
                     /locus_tag="KPG13_000752"
     CDS             30290..31252
                     /locus_tag="KPG13_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0984496.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(31374..32036)
                     /gene="zmp1"
                     /locus_tag="KPG13_000753"
     CDS             complement(31374..32036)
                     /gene="zmp1"
                     /locus_tag="KPG13_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG0984497.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(32136..32221)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(32407..35325)
                     /locus_tag="KPG13_000754"
     CDS             complement(32407..35325)
                     /locus_tag="KPG13_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG0984498.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(35443..35528)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(35655..36833)
                     /locus_tag="KPG13_000755"
     CDS             complement(35655..36833)
                     /locus_tag="KPG13_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0984499.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT        12266 a         6186 c         4315 g        14217 t
ORIGIN      
        1 aaattagagt ttaaaattaa aaattattct aatacatgta attgataaaa aataaaatat
       61 aagagtagct actttaaaat aaatgctctt atatgattag atatttttgc ataattaaac
      121 gataagaaat caaatttaat tttacataaa tagaatatat atttttatat aataattgaa
      181 aataaaagag gatacctcat aatgataaaa gaatcacttt gagataccct cttacaaaca
      241 actaaatcaa taattttcta tctttcacac tcttgatttg caactgtaca agaagtttta
      301 caagctgatt gacaagaagt ttgacactct ccacatccac cttttgcagc actttgcttt
      361 aaagtagctt gagataaagt ttttacatgt ttattttcca ttttcaacgc ctccatttct
      421 aattgtttcc tattgattat atcataaaac tactttaata taaactctat ttaaagttga
      481 cacccaatac gcctccaatg cctcctgctg ctgatattaa caataactta taaatcatcc
      541 ctacttcaaa tacaaacttt tcttgtgcta aaaacacagt caaaaaaata catactatgt
      601 acaaaagtcc tactaaaagc ccatacataa gtcctttttc tttcatgtgt tttgaagtgt
      661 aaaaccctcc tatagctgca gaaatagtag ttataaatga tgagaccata gttatattat
      721 ctccaccaat atctgtaaat gtcaaaaata agttatacac taataaaaca gccaaagtta
      781 ttatatatgc atatcctaga cctttaaaaa tataattaga ttttcccata aaaaaacctc
      841 ctctcaaatt aatatatatt cgagaaaagg cttttaaatt actaatttag tcttcttcta
      901 tttcttcttt tttagattta acagttccta tagcccattt ttcaaatgga actttagttc
      961 tatttggtcc taattctaat attacaacat catcttcaac tttagctaca ttagctacta
     1021 ttccacctat tgtaatgacc ttgtctccta cacttaggct acttctcatt tcttttaatt
     1081 gtttgtcttt tttcttttga ggtctaatca ttaggaaata aaagattgcg attactacta
     1141 cccatagacc tatactcata attatttgtt gctgtggcat taatttttca ctcctttgta
     1201 aattatactc ttttgtaaat cataataaat gtttatataa caatttactt tctctatctt
     1261 tgagtactat tgaatattat atattaaaat gattataatt aatagtgcaa aacctatatt
     1321 tcatatccat attttgcaaa aaattcattt ctaaaatcaa gtaatctatc ttccattatc
     1381 gcttgtctta tttgtttcat taaatttaat aaaaagtgaa gattatgagt agttattaat
     1441 cttgcaccca gtatttcatt tgcttttata aggtgtctta tataagctct tgaatagttt
     1501 ttacaagcat aacaatcaca ttcagggtca agtggagtaa agtcttcagc ataagttgca
     1561 tttctaacta ctactttacc ttggctagtc attgcagttc catttctagc gatacgagta
     1621 ggtaggacac aatcaaacat gtctactcct cttataacac cttctactaa gtcatctgga
     1681 ctaccaactc ccattaggta tcttggttta tcctttggca taagtggagt tgtatgctct
     1741 aacacgtcat acataagtgg ttttggttct ccaacactaa gtccaccaat agcatatcct
     1801 ggtaaatcaa tattagttat ttctttcgct gattgttccc ttaagtcttt atacatccca
     1861 ccttgtatta taccaaataa agcttgcttg tcggtattat tatgggcatc tttacatctt
     1921 tttaaccatc ttgttgttct ttctaaagaa ttttttacat attctctatc tgatgggtat
     1981 ggtgcacact catcaaatgc catcattatg tctgaaccca atgcattttg tatttccata
     2041 gctttttctg gagttagaaa atgttttgaa ccatctaagt gtgacctaaa ttctacacct
     2101 tcttctttta tctttcttaa aggccctaga ctaaatacct ggaaccctcc actatctgta
     2161 agtattggtt tatcccaatt cataaactta tgaagaccac cagctctttt tataagttca
     2221 tgacctggtc tcatatataa atggtatgtg ttactaagta ttatttgtga accaatctct
     2281 tttaattctt ctggtgtcat agattttaca gttgcttgag ttccaactgg catgaatatt
     2341 ggtgtttcaa taatcccatg tggagtgtgt aatcttccta accttgcacc actctgctta
     2401 caagtcttta tcaattcgta tcttactgcg tacataaagt tctccttaat catatttatt
     2461 tctcattatt tcttaagata tatttgtaat aagctattca tataatttat tgtctaaata
     2521 gatttatctt cgtcttcttt gttctgttgt tccttaattg cttcttcatc atgaatactc
     2581 attatattaa gagcactctt gtcttttgtt actaaaaact tttttatagc aaatagtcct
     2641 gctatagcta taactcctat taaaacatct atcatacctt catttttagg cagtagcaac
     2701 atttttctag caattgcgta aagtaatgcc tcaataaatg ctcctggtat atgaagagat
     2761 agcattacta caagttcaac acctattact aaaagtaata tttgtgctaa aaaatcatta
     2821 agctgactgt attgtactgg attttgaaaa tcaactatat atgctcccca cataagtctt
     2881 agaacatcta tagttcctaa aaatactgct attagaacta ctactgctaa aacacgttca
     2941 aatataaaag caatctttaa tatagggtta ttcttttttt gtttttgtaa actatgcatc
     3001 tattacctcc ctactttata atcatagcat ctccaaaact gaagaatcta tatctttctt
     3061 ttactgcctc attatatgca tttaaaacat tatcctttcc acaaatagca cttactaaca
     3121 ttattagtgt agactctggt aagtgaaaat tagtaataag tttatctaaa actttaaatt
     3181 tataacctgg atatataaat atttctgtcc atccactagt ttctttcatc tttccatcct
     3241 cattacaagc tgattctata gttctacaac tagtagtacc aacacatatt acattttttc
     3301 cattttcctt tgctctattt attttgtcag ctgcttcttg gctaaccata taatattctg
     3361 aatgcatctt gtgatttaaa acatcctcta cttttactgg tctaaaagtt cctaagccaa
     3421 catgtagagt tacaaaggct atatctacac ctttttcttt tattttattt aacaattctt
     3481 ctgtaaaatg taatcctgct gtaggtgctg ctgctgaacc attatgcttt gaataaacag
     3541 tctggtatct ttctttttca tctagtctag cagtgatata tggaggtagt ggcatatttc
     3601 caagttcatc caatatttct tcaaaaattc catcataatg gaattttata attcttgaac
     3661 cttcatctga taaatctaca acttctccaa ttagctttcc atttccaaaa gaaaatttag
     3721 tccctacttt tgctcttttt cctggtttta ctaaagcctg ccaagtatct tcctctgttc
     3781 tttttaataa taaaaactct attttacctc cagtttcaag cttctctcct attaatctag
     3841 ctggtataac tctagtatta tttaatacta agcaatctcc tggatttaaa tattctatta
     3901 tatctctaaa tactttatgt tcaatattac ctgtctcttt atctaatacc atcaatcttg
     3961 agctagctct atcttctata ggaacttgag ctataagttc ctgtggtaag tcaaatttaa
     4021 aatcacttgt tttcaatgtt aaacttcctc tcttaaaatt tctccagcta caataatatc
     4081 aaaaaaacaa cttattttcc acaatattac cataatctat caagaaattg ctatttttaa
     4141 cagacaccat atccacaata tatagatatt tttccacgat atacatgcct tatccacaat
     4201 atatagatac aatacacaca atttttatcg tgtttatcca cagtatctac agatataatt
     4261 accattttgt ggatatgttt gaatttttag ccattttaga aatattttaa tttaatgact
     4321 cttttatttt ataaatactc gtataatcta tttttatttt tcattgggat atggaatttt
     4381 taagtgttca tatgctaatg gcatagctac tcttcctcta ggacctctat ttataaatcc
     4441 taattgaagc aaatatggct catatacatc ttctattgta tttctatctt ctccgataga
     4501 agctgccaat gtatctaaac ccactggtcc tcctctaaat ttttctatta tagtcataag
     4561 aagcttctca tctacaaaat cgagacccaa actatcgact cctaaaagtt ccagagcatc
     4621 ttttgccact ttatcagtta tttttccatc agctctgact tgtgcaaaat ctctaactct
     4681 ttttagtaat ctatttgcta tccttggagt ccctcttgaa cgttttgcaa gttctaatgc
     4741 tccattagac tgtatctctg catctaagat gcttgaagac ctaagtacta tttttgaaag
     4801 ttcatcaact gtataatagt ccaatttaca aataacacca aatctatctc taagtggatt
     4861 tgtaagcata ccagctcttg tagtagctcc aataagtgta aatttaggaa ggtcaagtct
     4921 tatgcttcta gcagatggcc ctttccctat tattatatct aggcaaaaat cctccattgc
     4981 tggatataaa acttcttcaa cacttctatt gattctatga atctcatcta taaataaaac
     5041 atcattttca tttaaattcg taagtatggc tgccaaatct ccagctcttt ctattgcagg
     5101 tcctgacgta attcttaaat ttacccccat ctcattggct attatacttg ctagtgtagt
     5161 ttttccaaga cctggtggtc catataaaag cacatggtct aactgctcat ttctcgattt
     5221 agctgcttct ataaatatac taagttgttc tttggatttt tcttgaccca aataatcttc
     5281 taaagttttt ggtcttaaac tgttttctat atctatatct tccatcttca ttgtagaagt
     5341 tattatccta ttttcatctt caaaaccttg catttatacc cctcctctcg tacttttgaa
     5401 aatctacttt atatattttc aaatttacat aaactcctta aatattgttt taaaatccaa
     5461 atattataaa atccaaatac tatttcaagg atttactcat tatatatgta agtgctttct
     5521 ttataatagc ttctgtattc ataccatctt ttttacattt ttgaactgcc tcttttgatt
     5581 ccgacaaagt atagcctaaa gctacaagtg catctacaga ctcatcttgt gatatcaagg
     5641 taggtttttg tgataataac gttggttcaa actctatgtt atttttgtct accttatcct
     5701 taagctctaa gacgattctt tctgctgtct ttttaccaac accaggagct ttggataatt
     5761 ttccaatgtc ttcacttaaa atatatgctc ctaactgtgc tggtgatgca aatgatagta
     5821 ttgaaagcgc caccttaggt cctattttag atacagatgt tagtaattca aacatcttaa
     5881 gttcttccct acttacaaaa ccacaaagac tcatatcatc ttctcttaca attaatttag
     5941 tatatatttt tgtggcttct cccacttgta aattcattat tgtattggat gatacattta
     6001 ttttatatcc tatcccatta ttttcaacaa ctatactgtc tatataaatt tcttctactg
     6061 taccttttat ataactatac atacttacca cctatattct ttagagtttt ttcaagtttg
     6121 tttgaatgag catggcaaat agccacagct aaagcatctg caacatcatc tggttttgga
     6181 acttttttta gacttaaaaa tgaagttacc atctgttgaa cttgtgcctt atctgctctc
     6241 ccatatccaa ctacaccttg ttttacttga agtggagtgt attcatatac aggcttccca
     6301 ttatgagcac atgcaagcat agtaacacct ctagcttgtg caactgttat agctgttttt
     6361 acattcttgt taaagaataa ttcttccatt ccaacttcat ctatattgta attctttact
     6421 attgtatcaa ttcctttata cacaagttcc aatcttctcg atatattcat atgggcaggt
     6481 gttgtaactg ctccataatc gattgcttta aacttgctat ttttgtattc aattatacca
     6541 tatccaacta tggctatacc tgggtcaatc cccaatatta tcatttttta aaattccccc
     6601 taaaaataaa agaaatacca aaatgtattt ctcataaaaa cttatgttct aaatcattat
     6661 atcataaata atattaacaa atcataaaat gctctatgta aaatataaaa tcaaataaaa
     6721 aagttatatc aaagcaagat tttacctcaa tataactttt atatttatta caaataaatt
     6781 attgtatttt tataggaatt aaatgtgact acaattttaa tactaatcta tttaatatta
     6841 aattatttat tattaattta cttagtattt aacttaaaat aatattaatt tactattaac
     6901 ttgcaaagtc ttctaatctt gaataggcct cttcttcagt atccataact acaccttttt
     6961 tgatttcatc ttgaactgtt ttaggaagtg aatctactct tatttttgtt ttttctacta
     7021 atgttttact tccgctatca tcatatttat atagtgctat atttccatta tcattttcaa
     7081 tagtatattt accttttata gtaacatcca ttgcttgtac atctttatta tcactatatt
     7141 tagattctac caatatgatt tcatttttat ccatactctt tattttttta gttggatatt
     7201 cttttttaaa atatgatact atttctgact tcgtcttatc taataatgac tctggaacta
     7261 ttcctgccat tgtaagtgtt ctcttatagt tagtttcatt ttcgccttca ctttgaagcc
     7321 aaacttcaca atttttattt aatccaaaag cttcttttga cttttcattc atattcgcac
     7381 tagtacttac aacttcacca gtttgaactc cattgtgttt taatcttgtc cctatataaa
     7441 ctccgctact taatacaaat actccaacca gtattaaggc agatattttc caaggaaata
     7501 attctttttt cttcttttca aacattgatt acgcctcctt tttctattat tttctccaag
     7561 ctgtaagttt ttatacaatt taggtaaatt ttagaagaaa atatatttat atttcaaatt
     7621 actctaagaa tagatatttt ctaaaaggta gttttctctt aatggttata ttcttgtaat
     7681 tatatggaca taatttataa taaataatcg tttaggaggt aataactttg gaaagaataa
     7741 actgtaatgt aattaactgt tcacataaca acagccatat atgttatgca gataaaattt
     7801 ttataaatgg tgaaaaagct cacaatgaca gacatacttg ttgtagttca tttttagatg
     7861 aatctattta tagtaattta tctagcaacg ttaataataa aaattgtgag gaattagttt
     7921 gtactgtaaa aaattgtaaa tacaacaaag atggtagctt atgtacactt gataatatat
     7981 tggttgctcc tgaaactgat agagtaaacc tttatattga aacatgttgc tctagtttcg
     8041 aatgtaaata gtcatagtat aaatataagg gtgtcaaatg taattaaact aacatagtgt
     8101 agagtatcta caactattag tttgattata cttgacaccc ttatttattt taaataaaat
     8161 ttcattatta taaccatgtt tataatgttc taactgtaat taaatttgat aaatttctat
     8221 aaattggtat aatcaatatg taaattgaat ttagtaaatt aatcataaaa ataaaatatc
     8281 gagtatatag tgttatacta actagaaatg aggtgcttat gagtacattt tatattcaat
     8341 ttctttattt tgtaatttat agctttatag gatggtgttg tgaaactaca tattgttcag
     8401 ttttacaaaa acaatttgta aatagaggtt ttttaaatgg tccattttgc cctatatatg
     8461 gatttggagc attagcaata gttgctacct tgactccttt tgtacacaat attcctttgt
     8521 tatttttgtt tagtataatt ataactagca taatggagta ctgtacgagc tttattttag
     8581 aaaaaatatt taatatgact tggtgggatt attctaaaca taaatttaat attcatggaa
     8641 gagtttgtct ggaaaactca cttatgtttg gtgtattatc attgattgta atgcttatag
     8701 tacatcctat tgtggttgac tttattaact ctatctctaa aaatgtgcta tttatatttg
     8761 ctatatctat agaaatttat tttgttttag atttagttat cacagtccat acaattttac
     8821 aattaaatgg taagttaaaa caaatcaatc ttataattaa ggaattaaaa gataagaaag
     8881 aatactacaa acttattaca caagaaacta tagaaaacaa acttgaatct ttagttgaaa
     8941 atagactgga tactttggaa gataggtttg aaatacttaa agaaaaagaa aattatcgct
     9001 atgctaaaaa tcgtattgat gagctaaaaa ataaacttaa tactttgctg tctaaccaca
     9061 aattacttca tagacgtata atcaaagcat tccccaacat aagctctaat aagcatacag
     9121 atattttaaa taaaatcaaa gaaaatatta aatcaattaa aaaagataaa taataaaata
     9181 aacacataag ttaaataata aaaatactaa tacataagat taaggtttag aatatttttt
     9241 gtaaaatact ctaaacccta attaatatac tattattttt aatcttaatt tataatttta
     9301 gtttatttta attttaactc agtaaattta agcgtttaaa cttcaataca ctaatatact
     9361 accccatcaa tatctcaact gattctctaa gtgtgctaat ctctcccaca tttccaggga
     9421 aaattatata tggcatatta ggaaatttgc tttctttgcc tgtcatccat actggtatac
     9481 caggttttat ctgtcccatt actgtagctt tttttactct tagtgcttta gtaccaacat
     9541 cactagaagt aattccaccc ttagctataa taaaatttgg tcttacatta agcataccaa
     9601 ttatactggt aactgcatct gatatttcta cagaaatcat aagctgttta tctttatcat
     9661 tcgtatccaa gtcaaaacgc tctcgtctag tataaacagc aacagtttta ccactgctta
     9721 ttttttcctc tacaactttt ataactcttt ttacctcatt ttttagacca ttttcttgaa
     9781 gaactaaatg ttgattaaat tctataaatt ctatcggata tttacaattt ttaagttctt
     9841 ctaactgttg ggtagtctta ttcacatgtg aaccaacaag tataattcct ccattggtat
     9901 taccttttga tactaattca tccttagtaa gtagttcctt gtcactcact ccgccaagta
     9961 cttttgttat ggcagcagcg cttctaaaca taaattcctt accaccattt actgctcgta
    10021 taaacgcaat tgtaaatacc tttacatcta cataatcggt tgcatttaca ataattttat
    10081 tgaatccttc agctttttta agtttttctg ttatagcttc aatatttagc tctctaagtt
    10141 cttttgacga aatatatatc atatcactag atttgtactt tccatttgtc ttttcttcac
    10201 accactcacc tatatcagat gatttatatc caaaagattt atcttttgca aattcagtca
    10261 ttcctgctgg tacaagtatt tctccttctt taacataatg tacattatct atagtaaacc
    10321 taccaccttc tttaaaaaat ggcattatta tttcaccctg aaataacttc cccgataatc
    10381 tctctacttc gctttttaat aaattagttt caagaggata atgtcctctc atagtagaat
    10441 cactacgact tatgattata aaatccttat taactttctt tgatacatcc acaatattct
    10501 ttgaaatttc tttatgtgct tttgtagttt gagcaacagt aaatcccctt gaattggtaa
    10561 gtataaaaaa catactattt ttctcattaa agccttgttc catactatcc ttatcccaat
    10621 cagtataaac agaaatatca tgaactgttt gtacacctgt tgggtcatca tctaatacaa
    10681 caatttttct atctaaatgc ttaagctcat ctgcaagaag tttatttaat ctatcctcat
    10741 ctacttgttt aaaagtatta agtatatcta tgctaattgg ttttacttct ctcatttaaa
    10801 acgccccttt atgtaatttg tatagtctaa atataattta tatgatataa acaatagttt
    10861 gttatttata gacctggaag tggtataatt ccatttataa cactaagcaa taaaaccatt
    10921 ataaatgcta caactccagc aatggttcct ccaattgtcc aagagtttaa agtatcttta
    10981 actgataatc cactaaatct tgaaagtacc cagaatccag aatcatttgg aagggagaaa
    11041 ccaactcctc cagcacatat agcaagccct actaaaactg gtgacattcc agttgccaaa
    11101 attgtaggtc ctaaaattga tgatgttgtt actaaggcta cagtagcaga accttgagaa
    11161 attctaagca aggcacttaa taagaatcca agtactacaa ctgatatact aagaccagac
    11221 atagtatcaa ctaagaagtt acctatacca cttgcattta taacactgcc aaacgcacca
    11281 cctgaccctg ttatcaataa tattagccct gctgaatcag cagcttcaat aactacatct
    11341 cccataggtt tatttatgta tttctttaaa aatcctattg ccaccaatat tccaagaagt
    11401 attgctatgt ttttatctcc tataaatgca agtactccac tcattgtaga acctttttct
    11461 aagaacatac taaatacatt tgatataagt ataagaagta ttgggaacaa taaagaaaac
    11521 atagaaattc caaaacctgg tactttagaa gaatcatcat cttcaatttt tatgctatct
    11581 tttccctgct ctaaattttt ttctctatca ttctcatcaa tagcataagc tggatatttt
    11641 tcaaaacgtt ttccataaat ccatccacct ataagagctg ctggaaaagc aacaattata
    11701 gaataaagta taaaactacc tacatttgca tttacatttg ctgcaactgc caaaggtcct
    11761 ggtgtaggta ttacaagtgc atgacctact atagtcccaa ctcctaaggc acatacaaaa
    11821 accattagag atttcttagt tactctcgaa acatacttaa ttataggcat cattataaca
    11881 aaagctgcat ccataaaaac tggaattgaa attaaaaaac ctgctgtagt tactgctaaa
    11941 gcactgtttt tttctccagt cttagctaac aacccctttg caatagattc tgttgctctt
    12001 gattctgaaa gaatatttcc aaatattatt ccaagtccaa ttacaatacc tatactacct
    12061 aatgtatttc caaatccagt cgatattgtg gttgatattt catttatagg cattctaacc
    12121 ataaagcctg taattataga tgttatcagt aatgccaaaa acggatttac tttaaattta
    12181 ataatcgata caagtaaaat tcctatagat ataacaaatg ctatcaaaag tccatatcct
    12241 gtaatcataa tctttacccc tttatcattc tactttatta tttatcaatt ttatttttct
    12301 tttactatga taacttaacc tcgctgtatt tatataatct aatcctaata atatgtattt
    12361 aattatattg atatatttaa atattaatta tcgtgttttt ttatttcaac acctgccaac
    12421 ttttcaaaat actgtgctat tccactatgg tcatcttcca aatgtccatg tacttttaat
    12481 gtctgcataa tttctaataa ttgacttgtc ataggtagtg gtacatctaa attgtgtgct
    12541 gtttgcatta cattcttaat atctttcaaa ttaatagata tttttccacc tggtttaaaa
    12601 tcacgattca taatcattgg tatcttcaca tccaatattg tactaccagc taggcctcct
    12661 cttattgcct tatatacttt ttctgggtct gcacctgctt ttgctgcaag tacaaatgcc
    12721 tcagaaactg ctgctatcgt caaattaact attacttggt ttgtaagttt tgtaacactt
    12781 ccactaccat tatctccaat taaaatagat gagcttccca ttatattaaa aaatggctct
    12841 actttcttat aaatttcttc cttaccacca gccataaaag caagtgtacc atcaatcgct
    12901 tttggctccc ctccacttac tggtgagtct ataaattcta agcccatatc ttttaattta
    12961 tttgcacaca atattgattc tgtaggagta actgaactca tatcgactac tatacttcct
    13021 tcttttaaag tttttgccaa tccatccatc ccaaacaata tatcctgcac aatagttcca
    13081 tttggaagga ttgtaaatac aatatcacat tcttgaccca tttctaaaac acttgcgtat
    13141 tttgctccac atgatataag ttcatccaca gcagatttat taatgtcata caccaaaaga
    13201 ttacatccat cttttaataa attttttgcc attggctttc ccattatccc taatccaata
    13261 aatcccaatt tcattttata accctccata tcttcacatt tttgaaaacg ttttttatgt
    13321 tttagagtat actacatacc agtataccgg tcaatataaa atataaaagt atttatttta
    13381 acaataaata gtaattttta aagtcattta ttatatttag tcatcctata taagttttaa
    13441 tacatactat tgttttcaaa tataatatac tacttttaca gttttgtatt ttaactaatt
    13501 tcaattagtt ttaattaatc tgcctttaat gttaatcatg cttcacactt caataatttt
    13561 ttatattttt taatcttatc tgcaatttaa attgcaaaag attgacctta ttttcactat
    13621 gttgtatgct taaaattata taagtttttg tatttttata ttgtttaggg gggtattatt
    13681 aagtatggct acatttaaaa gattacaaga acaagcatat gactacttaa aagaattgat
    13741 actatctgga gaaatgatag aaaatgaaat atattctgaa acaaaacttg ctagtgaaat
    13801 aggtatctct agaacaccta taagagatgc tcttcaaaga cttagtcaag atggatttat
    13861 agatataatc ccaagcaaag gatttagaat ccatcaaatt acagcaaatg aaattgtaga
    13921 gatattccaa atacgctctg caatagaagg attttgtact tttttaatta caagtcaata
    13981 caaagaagct agagcagtag aaactataag taaattaaaa catcttttag ataaacaaaa
    14041 agatatttta ttaggtgata aaaatttaaa ctcatttgca gaatacgata cattatttca
    14101 cacaactata gtttcatatg ctcaaaatac tgaatttgac aaaatgttta acaattatat
    14161 gtacagaata aaaaaattag ctctcgattc attgtcacat gaaggaaggc ttgaaacaac
    14221 attaaaagag cacacagata tttttaataa tattgcaaat ggatgtattg aagatattta
    14281 cagaactact ctcattcata tggaaactcc taaatacatt aatttagaag atttttgtaa
    14341 caataagtta ttttagtatt atactttcat ttattaaaaa tatatctatc tcaaattaat
    14401 ttcctaattt ataaaaaata ttctataatt atattgtaac taatacttat tctaaggagt
    14461 gatttttttg aacgaagatg ttgtaactaa tgaaactatt gacaaagatt atgctattga
    14521 agaagttcgt atggcatgca aacattttgg tgatttatat ttttattttt ccaaagtgtt
    14581 atttgaagag tttggtgaag ataaaactag cgaaatatta agaaaagttt tatttgaaag
    14641 gtctgaagaa agagctattg ctatgagaga gagagctcat gaaaatggag atgaattaat
    14701 cgcagacaat attattagca caactgatgt tccatttcta ggatgggttc ctgaatttga
    14761 agagttacat tgcccctatg gagcttcttg gcttttaaga tttgaggaaa atccttggtt
    14821 taaaaaattt gcttctcttt actgtgatgt tacagatact actgtagctg aagtatttac
    14881 aggagatacg tcccataaaa taacaaaaaa cattctatgg ggagataaaa gttgtgaaag
    14941 aatatatttc catgatgaca aggtatctga aggtaaatat acttatggaa ataaaaactg
    15001 taaataaatg atttaaattt tctatattca tatactatat tttgtacttt ttcatataaa
    15061 tattataact agtcttttat tttacttttg tttaacctaa agataattta cttttaatct
    15121 atatgaatta tcatataaat aatcagaaag ttatctttat aaattatagt tttagattta
    15181 taatttataa aatatttatg ttctttgtaa aattttttag aagcttctaa tgttatgcta
    15241 taaaactatc ttgagaaggg ctgattgaat gtttaataaa atatatgaaa agaaagaatt
    15301 tatagttttt caagttaaaa aaggttatgt agtttataat actagaaaga gttttgaaga
    15361 aggtcataca catttaaagc attttgaagc agcaaagaca gctattgatt tagcaataaa
    15421 taaaaagatt cctagaagca aagatggata ttatctgact agtttaattc gcataagtga
    15481 tgatggttat tacattgata agttaagcga attgttatat gttagagaac aaaaaggtaa
    15541 aaaagaaaag tactgtaatt ctggttatca gatgtaacaa gactagttgt gaattataag
    15601 taccaatcat aagtaccttt agtcttaaaa tcaaagagta tcagtaaatc tataaaaaaa
    15661 caacttactg atactctttt taattatatg attaatattt gaatttgata caaaatttta
    15721 tacaaaagtt tatgttatct attctgattc tttgtctgca aattggctat tataaagctt
    15781 ctcataaaat ccaccctttt ccattaattc atcatgattc ccctgctcta taatacttcc
    15841 attattcata actaaaatca agtcagcatt gcgaattgta gaaagtctat gtgctataac
    15901 aaaactagtt ctaccattca taagatttct cattgctttt tgaagcataa gttccagcct
    15961 agtatcaaca gaactcgtag cttcatcaag tatcaatatt gaagggtctg atataaaagc
    16021 tctagctatg gttaaaagct gtttttcccc tagagatata ttagatgctt cttcatttaa
    16081 aaacatattg tatccatctg gaagagtggt tataaagtga tgaacatttg ctacttttgc
    16141 tgcttgtata atttcttctt tagtagcccc aaatctacca tattctatat tttcaaaaat
    16201 ggttccatta aataaccatg tatcttgaag caccatacca aacattgaac gcaaatcttt
    16261 acgtttcata tctcttatat ctactccatc aattttaata gcacctttct ttacatcata
    16321 aaacctcatc aataagttta ttaatgttgt tttacctgct ccagtaggtc caacaatagc
    16381 taccatctga ccacttttta cttctgcatt taaatcttct attaaagttg aattttctcc
    16441 atatccaaaa ttaacatgct caaatgtaac atttcctttt acattttcta tcttaactgg
    16501 attttcaaca tcaggtattt cctcttcttc gtctaaaatt tcaaatacac gttctatagc
    16561 tgcaaaagaa gattgtatta ctgtagataa ctgagtcatt tgtgaaagtg gctgatttat
    16621 ctgccatata taacgcacaa atgcttgtaa atttcctact gttattcctc cacttactgc
    16681 atatatagaa ccgacaactg ccacagctgc aattccaaag taagttacta atgaaaccat
    16741 aggattcatc atacttgata taaattgtgc cttaaatcca ttttcacaaa gttcttgatt
    16801 tactttttta aattctttta tagaatcttc ttctttacca tatagtttta tttcattaaa
    16861 tccagtatac atttcttgta cttttccatt taattttcca agagcatttt gttgcaatga
    16921 aaatctactt tgagattttt ttactacaaa tttagataca aaataactaa ttggaattat
    16981 aaaaagagct ataagagcca tatatatatt aattgtaaac atcattgaaa gtgccaaaat
    17041 tatgactaag attgagttta caatttgcat aaaactctgc tgcaatgcat ttgatattgt
    17101 atctacatca tttgatatac gacttaatac atctccaaaa ctattactat caaaataact
    17161 aataggcaat cttcttattt tattttcgac ttcatttctt aaatctctca ttgtgttttg
    17221 aatagcattc gtaagtagaa aacttgaagt atacgtaaaa attgcattgg caaaatatat
    17281 acatgctaaa atcaccaata tttttgttac ataatcaaaa tttacggaag cccctggtac
    17341 tccttttgcg atactaatta catctttagt caattgtgtt attatcaatc cttcagtttt
    17401 aggtgcaagt gcattcatag tagctgctga aattatacat ataatagcta taataaagga
    17461 aaatttatat ttctttacaa atggaagaag tctaccaatt gttttcttaa aacctttaga
    17521 ctttttcatt attctaattc ctcctttttt aactgagatg ttgcaatttc atagtatatt
    17581 tcacagtttt ttaataattc tttatgagtt cccattccta caacttgtcc ttcattgagt
    17641 actataatct tatcagcatc aataatagaa ctaattctct gggcaacaat caatacaatt
    17701 gcatcttttg tttccttctt caatttagca cgaagtattg catcagtttt aaagtcaagt
    17761 gcagaaaaac tatcatcaaa aatgtatatt tcaggtcttc ttataatagc ccttgcaata
    17821 gaaagtcttt gtttttgacc tcctgataca tttgctccac cttcactaat caattcatca
    17881 aactttctag gtttttttga tataaactca tatgcttgtg caacttttgc ggaatattct
    17941 aattctgact ctcctgcctt atgtttccca aatctaatat tatttgctat gctaccagta
    18001 aaaagcagtg atttttgagg tatgaatcct atcttttttc ttaaagcttt taaatcatat
    18061 tctcttatat ctactccatt tatcttaatg ctaccttctg ttacatcata aaatctaggt
    18121 atcaagttaa ttagagtact tttcccacta ccagtacttc ctataaaagc gactgtttca
    18181 cctgtttttg cagtaaatga aatatcctta agtacagaag cttctccatc tggataggta
    18241 aaagttacat tatcaaattc tatgataccc ttattttcag tatccttaat tccattttca
    18301 ggatttttta tcaatggttc ttcatttaat agttctttta ttctatttgc agatacctct
    18361 gctcttggat acataataaa taccattgaa aaaagcataa ttgaaaacat ggcatggaaa
    18421 agatattcca aaaatgcaac caactgtcca acttgaagct ctccaatatt tatcttctca
    18481 cttgaaatcc aaaatacagc caaaacagct atatttaata gtaaaaagaa tgctggttga
    18541 gtaattgaca taagcttaaa tagtttctta gaaacattag cataatatgt atttgttttt
    18601 tcaaatcgct ctgtttcata atcatcattt ccaaaagctc taattactct tattccagtt
    18661 aaattttctc tcgatataag gtttaattta tctagtcctt tttgttgttt tgaagatatt
    18721 gcctgagata cttttgctat tattgctact ccaataatta taaaaggtac tgatatagcc
    18781 aatactatag aaagttccac acttgttcta attgtcatta ttatacttat aatgaacata
    18841 actggagtta ataaggcagt ccttagtagt atattaacaa attgttgaat ttgaaatgca
    18901 tcattcgttg ttcttgtaat cattgaagaa attccaaatt tattgtattc agtatgtgaa
    18961 aattcttgtg atttctcaaa tatgtcattt cttatatctc tggttacact tgttgatacc
    19021 ttagcagaac aataacccaa caatatacta cctacaaccc ctataataga aataataaca
    19081 atcactattc ccatttgttt tatgtaagct atattctgat ttgcaatacc attgtcaatt
    19141 attttagcta ttatagttgg aatccccaac tcgactaatg caaaactaaa tatagccagt
    19201 atgtttaata ctattaataa cttataattc tttaaataac ttaaaattaa tttcatattt
    19261 ttctcctttc ttaatttttg agatagtgta atcatgtaat aatactaaat ttataaatgc
    19321 ttaattttat aaaaaaagga gaaaagagtt aacgcactct cttctccagc atattctttt
    19381 taatacaccc tccgacaagt cgcttatatg ttaacatatt tgacaagtaa tgtcaattca
    19441 tatagccaaa agtcttttaa atgcttcgtt tcataacatt gtaacaaatt ggcatgtaat
    19501 ttatttttgt tttttactgt attccaattt tacaagttct ccaatattac tagctttctt
    19561 aaaattcaaa ttagtcttat ctataccaga cttaaatagt gaaatccctc tccccaatat
    19621 tattgtcaat atatatatat aataatcatc aattaagtta tctttcataa ataattttac
    19681 tatatcgcct ccaccaacta tccaaatatc ttttttatcc ttctcttttc caatttgctc
    19741 aattaatttt ttaggttcta aattaattta tagtgtgatt attctatgta aacatcactt
    19801 ttttactcat tcaataatac cctattaata aatcctcaaa ctaatttaaa taaactttgt
    19861 ataaaattaa aaatatatta tataaataat ttaaaactac actaacttat tgaaaattct
    19921 aattattaca aataaatatt gctctctgtt aacgctagtt ctataatata attataagct
    19981 attaatatta taaagccgat attactaata taataattat aatacttata gttaatttac
    20041 ttttgttgaa aggtggtaaa taacatgaaa atttcagaag tcgctactcc aaattttttg
    20101 ctagatttag accagttaga aaagaatata gataaaattc aacaaatttg tacaaataat
    20161 aataaacaac tttggcctat gcttaagaca cataaaagta cttacatagc gagacttcaa
    20221 aaaaatgcag gtgctagtgg ttttctagtt ggaacacttg atgaaggtga agctctgata
    20281 aaagcaggtt ttgaagatat tatgttggct tatccttaca tgggagaagt aaatataaat
    20341 agaattgtga ctatggcatc aaaaataaat ttaatctgtg ctacagataa tatagaatgt
    20401 gctaaggctt atagtgaagc tttttctaaa tcaaaaatag agtgcaagct acttattatt
    20461 gtagattgtg gcttacaccg ttttggggtt gaaccagaaa aagtggttga acttgctact
    20521 gaaatttcta agctagaaaa catcaagatt gttggtataa gttcgcatcc aggtcaagtg
    20581 tatggttgta gttctccaga aggtgttcca gatgtttgtg ttcaagaaga ttcttctatg
    20641 gagaaagcat acaaagctct aactgataat ggatttaata tagaaattgt cgcttctggt
    20701 agtactccaa cagttgaatc tgaagctgct tctaaaacta taactgctgt aagacctgga
    20761 aactatgtat actatgatgc tatacagata ggtttaggtt gtgctactga agatatgtgt
    20821 gctcttactg ttgtgtgcac tgtaatctca aaaaatagca gtggatacta tttaattgat
    20881 tgtggaagta agtgccttgg acttgataaa ggtgcacatg gaaatagtag tattgttggt
    20941 tatggtaggg tatgtggaca tccagaactt actattgatt cactttcaga agaagttgga
    21001 aaaattaaag taaatggtga aactgatata aaaattggtg atagaataag aattatacct
    21061 aatcattctt gttccactgc aaacttgaca agtaatttat taggttttag aggagataca
    21121 atagaaaaag tcatagaagt agatattcgt gaaaattcaa aacaaatcat cctttaaaaa
    21181 cacatttatt ctgatattaa catgaaagta tatttttctt aaaattattg ctaataacta
    21241 atgttaatgt tggataaata aaaaacataa attctagaaa tataaattta aaagaagagg
    21301 ctgtctcaaa atagagataa gcctctcttt cattgttgaa attatgaaaa tatacttgat
    21361 ttcatacaaa aagagtatct catgaactaa aaagttcact ttgagatact ctcttttttt
    21421 aattaatata cacattaatt actatcttag tttattacta tatatcaatt actactcatt
    21481 aatccttgta tatattaatt cttatatatc atttatagtt aggttataat tttatgttat
    21541 ttacattaat cccataacag tgagtaaaac tcttactatc aacatcccca ctattgcatc
    21601 aaatatagga actgccaaca gtaacattct atacttagta ctggcatttg ctacaagcac
    21661 tattctggca taatgaccaa ctagagtacc cattaacata catggcataa ttaatatggt
    21721 tgcttgtggt gcagtgatta gaccttgtgc aaataagttt gcagctgtag ctgctcctgc
    21781 tgccttagca aagaatgctg ctatcaaaac tacaacagat tccccaggta gtccaaaaat
    21841 acccattact ggtccaaaaa tatttcctaa aatatcaact aaaccagtta attgtaaaaa
    21901 ctgcactatt acataaccca ttatcatggc tggtaatatc tgttcaacac ctatgtaaaa
    21961 accttttttt gctcccccca taaatatctc aacagcagtt ttcttaggtt tactatttac
    22021 aattacattt ccagtatttt gttgactcat actacacagc ctccttagcc ttattctttt
    22081 tatttcttac acctatgatt aatctaacta gattagcacc aagtatttta cagaatatct
    22141 ctataagtat tattggacct aatgccaata atgctattgg taaaagtgga gcttgagtat
    22201 ttattgtgtt cagtataaca gctgaaccag catattgata tgctacaaat atagttcttt
    22261 catcatctgt tatttcccca ctttcaaata attctttcgt cattattgag gctacatctg
    22321 aactagtaaa tgaagataca aatgctattc ctgctattcc aggtatacca agcagaggtc
    22381 taagcaatgg attaaatatc tttgatgccg ctttcattcc tcccatagat tcaactacat
    22441 ctataagacc tacagcaaaa cagacagttg gaatcaaagt cagagcaaat aagaaaccat
    22501 cttttgctcc cgttcctcct ttacccataa agtctacacc atctgcaata gtaccaaatg
    22561 aacctgtcag attagcaaaa tctaatgctt taaggaagcc ttgactatct ttaaacactc
    22621 ctgaaaataa tactatcaat acaaataaag ataaataccc tttccatgtt accttattat
    22681 ctccagctgt tacatccata taaaatcccc ctttatacct caaatttatc taagtttaaa
    22741 ataactgtag aaattaattt caccctctca aacatagaat ctactactgc atattctctt
    22801 gatgtgtggt tccattctcc tcttacacca aatgaacaga ttgtaggaac gtttgcaata
    22861 gttaaataac ttgcatctga actacctcca agtctcttag catttaattc tccaaatcca
    22921 ttttcttttg acacttcatt tacaaatgta tgaaatctca taacatcttc tgtagtttca
    22981 aatggcatca tttcacttat aaaattaact tctgtagaag ttccttctat gtaagtttct
    23041 ttacatatct ctctaacttg ttctttaaca ttctccattt cttctacttt ttcaaatcta
    23101 aggtctactt ttatactaca atcttctgga atagaatttt ccactcttcc acccttaatc
    23161 acacttacac ttactgtagt tccaacatct aaattagtca atttttgtat gcgtagtatc
    23221 ttattagcca tttcttctat agcatttctt ccaccttcaa aatcattacc tgcgtgtgtt
    23281 tctacacctt ttacatgtat attacatcct attcttcctt ttctacctac acacaaacta
    23341 ttatctacta atccagtttc catattaaaa gcacaaagac caccttttgc ttccctcaat
    23401 ataacatctg cacctgttga atccttatgt cctatctctt catctccaga aaatactatt
    23461 tttattggtc tttctttgta tcctatttta tttaatgctt taatagcata taaggaaatt
    23521 attataccac ccttcatatc taaaacacct gggccatatg cttttccttc tattatttta
    23581 aatggattct ctccaaatgt acctgtttca aagacagtat ccatgtgacc tgaaaaaatt
    23641 attggctttt tatctatatt ggaaccaagt gtacctatca atgtactacc attatctcca
    23701 acatctacta actcgcaatc taaaccttct ttttcaaact caaactttaa tctcttagcc
    23761 aatttattta cgctttcttt acaatgtgta tagctttcca tattaacaat ctctttccat
    23821 aatgaaacta tttcttctct attctcatca acaaacttag aaatttcatt taaatatgta
    23881 ttcataatct tctcctttat tttcaaataa aatgtatttc tttcctaaaa tctaagttta
    23941 ttttaggaaa tcttaaaaga ctagtcctta attacaaaat agcacaatta ttcttgacag
    24001 tcaacatatt tttcagtctt ttttatattt tctgattttt ctttcaattt tccttttgta
    24061 aaataaattg acgcgtttta tctactatta taaaatgagg ttataaattg attgattagt
    24121 tacaatactc tcgaaatttt aaaggaggtt ttatgaaata taaaattggt tttttgacaa
    24181 catcttattt tatgtcagat aagtttaaac aagctcttaa agaattaaat gatacttgtg
    24241 aaattacatt tattgagctt aaaggggaga atccttttaa taatttacct aatatatact
    24301 atgagcaagt agataatttt gatggatttg tatgcagtgg tatagttcca tatagtgaac
    24361 ttataataaa tataaaagac ataaaagttc cattaaattt tttaaagctt gatgaaaaag
    24421 atttttataa atacttattc aaattattaa atagccaaaa agatattgac tttacaaaat
    24481 catttatgga ttttttaaga gaggacaaca attattatga tatatattca ttaatagact
    24541 ctaataaatg tccatataca attaaagatt ttaatatccc tcaacctgta tatgacttta
    24601 aaaaattaaa tgataaatta ttggaaatac atttagactt attgcgagat aataagatta
    24661 acctttcttt tactagaaat tacatcatta gttgtgaact taataaactt ggatatgatt
    24721 ctatttacat ggttccatct acagaatcaa tcctgaatac atttaaatct ctggtgaatg
    24781 aaataacatt acaaaactta gacaaaaata aatctgcaac ttgtatagtt acagtaaata
    24841 gttcagagta cataaatgaa gatttgatat ttaaaaatga tgagatacaa aatcaaatat
    24901 ataacactat attaaattct ctatcaagac atggattcta tggagttcag gtcaaaaaaa
    24961 atgatatgaa aatagagata cacactacta aagaaatgtt aaacaatatg accaataact
    25021 atacagattt ttttatttca gaagatttaa aagaaattaa atatagctta aatataggtt
    25081 ggggcattgg aaatacaaat attcatgcag agcaaaatgc tcgtcaagct aatggtaaat
    25141 ctgcttcttt gaatggaaac tgtactttta tagtaaatga tactaatgat acaataggcc
    25201 ctttatattc aaataaaaat tctaataata ttgaagatag ctcaatagcc aataaagttg
    25261 cttcttttat acctctatct aatacaaatg tatctaaaat catgtgtatg attaatgata
    25321 gaaattcaaa taatgtgtca gcagaaatat tagcagacta tatgaacata actcttagaa
    25381 gtgctaatag aatattgagt atcttatata aagcaggaat tgcaactatt gtaaatacaa
    25441 aactcgataa tcaaagagga aggccaaaaa aatatataaa gttgatttct tatcttttct
    25501 aaataagatg cagaaattaa attcttaata tttaattttt taataaaagc aatgtatttt
    25561 ctctaatttt aaagaatcaa aatgtttaaa ttttattaga atatacaact ttttacttta
    25621 aaatacctaa accttttatc agtgaaataa accataatat atcgtaactt attagaaaaa
    25681 caaaataatt tcatatacaa ataataaaat agccactaca atccataatt tgcagtggct
    25741 actttttaca ctatcactta ttacttattt ttaaaaatta attgtatttt ttaaataatt
    25801 tatacacacc tattatcagt aaagcaataa atacaagtat atctataaaa ataattattg
    25861 taacatgtct gacaatattg taaataaaaa atatagagaa tatttctata gacaacgcta
    25921 caaaacacat aaaattcgtt aaataatctg caatagtatc actataaaaa gcatgtattt
    25981 gtaaccatct ccatataaat ggacgtaaaa gtattctgaa tacaacacaa atgacagggt
    26041 aggcataaat aaatttttta tctacataat tagctacacc attattccat tggataggaa
    26101 tttgaggtgg caacttttga aatactgtta ttgcaattta aacactacct ctctattcta
    26161 ttaatttata actataatat agtcttaaat attttcactc tataaaattg tatatttagt
    26221 atatcttaaa atcaaactaa ttatctttta taagttttca tacaataata tctcctttca
    26281 ataatatatc tttttttata tttaaaacta tcttattttc tataaaataa tgtataaaca
    26341 aggtttacag tcaagtattt ttattatttt tttgatttca taaaaaaagc atagcaaatt
    26401 atttgctata cttttttact aaattattta ttctatatct tatatgaact tattgtttaa
    26461 ctactacaca atactagctt taatcatcat attttttgca aacatactgg cttcatatat
    26521 ttcttgttca tctggatgac tttgagattc ctcatggtta tttaattgtt ggcgaatatg
    26581 ttcgttgtca ggagaaagtt ttaacatctc tttatatttt gctttcaatc cttctgctat
    26641 cttaccttga catatgaatg aacctagtat ttcattatct tttgggataa ttgattctat
    26701 cttttctatt atactagctc cataacttcc ttttttactt gctcccattg ttccaaatag
    26761 tgctatcttt ttattgtgaa tattttcaag cacttgtttt gaatttttat cacaaattcc
    26821 cttgtccacc caatagccta agaatactat atcataattt tcatagtcaa taccataatc
    26881 aactaattca tttacatctt taatgtcttt ttcttcttga atttcatcat atatagtata
    26941 agcaatcttt ttagtatttc cagttaaact tgaatataaa actaaacttt tcatgatgtc
    27001 tcctcctctt tatctaaata tttatctaaa tcaccaaaaa tatattaacc ttaacaaatt
    27061 atttattttt ataaggatat tctaatctag tatacatcca aactgagagc gtgtttgtaa
    27121 agcatctgca cacttcttat tacaataaca gcacttttta atctcatcta ctcttccatc
    27181 ttttactttc tgtaaccaat tactatctgc tagtaattgt cttgacatag aaaccatatc
    27241 tactctatta tttgaaataa tactttctat aaaatctggg ctagacaatt ttccaacccc
    27301 acatactgga agatttgtgt gtttctttac ttcatcagct aaatataaaa aacagccttc
    27361 ttctttaaag taaggatgat tattggttgg tatagtatct tctaattttg aatgatttgc
    27421 aagtgttaca tgaaaactgt ttacacctag ggattcaaat tttttaacaa aatactcaac
    27481 ttccgaaagt aaaactcctg catttccata atgaggattt tcttgtctta ttgcaaattt
    27541 atagtctatt ggaatgtcct taacttcatc tcggatacta ctaattattt cgcttgcaaa
    27601 tctacttcta ttatcacacg ttctaccata ttcatcattt ctattattaa atatacttga
    27661 tgaaaaactt cctacaagcc tatcaccatg aatttgaatc atgtcaaaac ctgctttttt
    27721 tgcattcaat gctgttactt taaataaatt tataatattt ttaatttctt ctttgggcat
    27781 atttgttatg taatctttta ctccgtcatt cattattttt tttatctcat catgtgaagt
    27841 aattcccata ttcatagtat ctttaattaa attaacatca taatctgaac aaaatagttg
    27901 tgctgatacc tttgcaccag aattgtgtat ttcatctaca attttcttgt atttcatgac
    27961 tccatcttca tccgataggc ttatagctac cttatgaaaa ctaggtctaa tagatacatc
    28021 tcctagagta atcagagcta ctcctgactt agcaatatca gaaaactttt taattttttc
    28081 ttccaattta agacccatgc ttgtaggtgc aaaagatact ctatttttta attccaaatt
    28141 tccaatttta attggtttta ataaattaca atacatccta ttcttcctcc acagctgttt
    28201 ttatatcctt taccacatgt aaattcttta ccagttcttt atattctgta tccgagaact
    28261 ctttttttat gtgattatcc cactttgaaa atatgttttt tatatcccct aatacttcct
    28321 cacccttatc tgttactctc aactgaaaag ctctactatc attagggttt tggatttttt
    28381 ctatgtatcc atcttgttct aacctagata tagttcttgt tgtatgagct ttatcaactt
    28441 caaattctgt agaaacatca ttcaaagaac aattcctatt tttattaata taaattaaat
    28501 agaaatatag accattcgta agtttatatg gttttaaaca tttttcacaa taaagtttaa
    28561 agtttttttg caagttacaa ataatatatg acaaactttc atactgtaaa tttttaaaat
    28621 ccatcttaaa tttcccttct ataattataa tatgtttgac tatatcaact atattatatt
    28681 aaacaattaa tatagtcaag aatattttat tctctaaatc tatgttttat taattcttta
    28741 aaaaactatt atataaaata atcatgataa aattagaaat tggaattttt aatatgtact
    28801 gtacataaat atattttata taaaagttta tattttcaaa caagtaattt ttcgaaaatt
    28861 gtctaaattc agtaaaaata tggtaatata tacataagca atataaatct ataaaaagga
    28921 gtgttacata tgatatctaa taaaatgcaa actttagttg caaatagttc tgttataaga
    28981 gctatgtttg aagaaggaaa gaaactttct gatatttatg gtgaggaaaa tgtttttgat
    29041 tttagtatag gaaatccaag tgttgaacct ccagagacta ttaaagcagt tataaatgat
    29101 attttaaatg aagaatctcc aaatttagtt catggatata tgaacaactc aggatatgag
    29161 gatgtaagag atgctattgc tgaacacata aataaaaaag atggattaaa tcttacaaga
    29221 gaaaatttaa ttatgacttg tggtgctgct ggtggtctaa atattatttt aaaaactctt
    29281 ttaaatcctg gtgatgaagt tattgctttt gcaccgtatt ttggtgagta taaaaattat
    29341 acagaaaact atgatggaaa attaatagaa gttcctacaa atatagagac ttttgaacca
    29401 gatttagatg ctctaaaaaa tgctattact ccaaaaacaa gagctttaat aataaacact
    29461 ccaaataatc ctactggtgt catatattct gaagaattat taaaaaatct tggagaatta
    29521 ttggattcaa aacaaaaaga atttaataca agcatatacc taatttctga tgagccatat
    29581 agagaaataa tttatgatgg tgccaaagtt ccttgtgtac taaaatacta tagaaattca
    29641 tttataggat actcttatag taaatcatta tctctacctg gtgaacgtat aggatatatt
    29701 gttgctaatg ggcaaatgga tgattttgat gatgttatgt cttcattaaa tgttgcaaat
    29761 agaatacttg gatttgtaaa tgctccttct ctttttcaaa gggttattgc aagaagttta
    29821 gatgcagaag ttgatgtaaa tatttataaa aagaattttg accttctata taatagttta
    29881 atcgatatgg gatattcttg tgttaaacct aatggtacat tctatttgtt cccaaaagct
    29941 ccaattgaag atgataaaaa attctgcaat gatgcaaaac aatttaattt attattagta
    30001 ccaggttctt cttttggttg tccaggtcat tttagagtat catattgtgt atcttatgat
    30061 aaagttaaaa gttctttacc agcatttgaa aaattagcca aattatataa tttaaaataa
    30121 ctaaatcaaa gtaattcaaa taattaacta ccttagtata gaatacttac attctatatt
    30181 aaggtagttt ataattttat gtactatatt ttatacatac catattattc caatatgctt
    30241 gaatattctt atctatagta ctaatataaa ttacaaggag gtgtttgact tgaaaaaagg
    30301 ttatgtttat ataatactga ctaccttatt ttttagttca atggaaatta gtctaaaaac
    30361 tgtcactaat gactttaatc ccttacaaat aactttatca agattttttg ttggcggttt
    30421 agtcttacta ccttttgctg taaaaagatt aagagctctt agtctttcta ttacaaaatc
    30481 agacttaaaa tactttgcat ttttaggttt tatgtgtgta gtagtaagta tgtcattgta
    30541 tcaacttgct gttctaaaca caaaggcatc tgtagttgct gttttgttca gttgtaatcc
    30601 agtctttgta atgcttttag cttacttaat tttgaaggaa aaaatatata ggcacaatgt
    30661 aatttcactt atattagaag ttcttggaat tatagttata ataaatccat tgcatacaaa
    30721 actaactctt agtggaatat tcttaacatt atcttctgct attatttttt caatgtatac
    30781 tgtatttggt aaaagaaaaa ccttaaaatt tggtggaata gttgtaacct gctttagctt
    30841 tatatttggt agtttggaaa tgttgatatt ggttttactc actaaaatta attttatcgc
    30901 agatatatta aataaaaata agcttcatgt atttgcaaat atccctattt ttagtggata
    30961 taccttacac aatatgccca ttatgattta tgtattcgta tttgttacag gtgttggtta
    31021 tgcgctttac tttatggcaa tggaagctac atctactagt cttacatctt tagtattctt
    31081 ctttaaacca gtaatatctc caatactagc attattaata cttaaagaaa ttatccctat
    31141 aaacatggtt ataggaattt tattgatagt aattggttca ataatttcta ttatacctac
    31201 gataattact caaatgcata ataaacacgt tgaagccttg tataaggagt agtaattgat
    31261 aatctatcat agttatacat aagacaaaat ttattagatt taaacgcaat ataaaagaga
    31321 tgctattata atagcatctc tttatttata tttattttaa atttattaaa actctattta
    31381 gctaaatttt gcaaaaagct ataagtttgt ggacaagctg attttaactt actatttgta
    31441 tcttgattta aatagtaata tgcaaatgat tctgcaaaaa attcttctgg atatacacct
    31501 aagtagttta cattacctaa agaacgacct tctttagcaa atatttgttt gaattgtgct
    31561 gatttagata tatcatttaa tactatatgg tctattgcat gtgctgtttc atgtaattct
    31621 aaatttattg catcatgccc tttacctttg ttactaaatc ctattctaag agctactgta
    31681 cttccaccta aacctggaac atcatcccaa gtttttccag taccttccca tcccttagga
    31741 actacacctt ttaaataagc atattctttt tcatctgtta actttccact taataatttt
    31801 atttttaagt tattttgctt taatgcattt aaatactttc catctatatt agctaatcta
    31861 tttaccatag cattagcttc gtttttatcg taatttccag ttggaaaaac tactatttga
    31921 ctaagtgtgt ctttattttg ttgtatagta gtactatctg catgtgcact tactggaact
    31981 gaacttatta agaatattga tattatagct attaataatt ttttacttgg tctcatatta
    32041 ttccctcctt tgagttaggc cgggagtaat aaatttcaaa ataaaaaaga tacccaagag
    32101 tatctttgta tatacaaaaa gaaattttta ctcttttcgg caactggctg gcatggttta
    32161 ctaaacttta gcccctatag ctttgcgtga ctaggtttcc ctagttttgc ctcatttaat
    32221 tttatatcta tattatagct tatatctttt taattgtcta tatccctttg gaactttttt
    32281 ctaaaaagtt attatttttt tacataaatc ttcaaaaaaa aatatcttct aacaatattt
    32341 tcattattaa atattgtttt agaagatatt ttttaatata acttatattt tttataatgt
    32401 tagggtctaa tttgtatttt tatttcttct taatacgata agtcctacaa tagctcctat
    32461 aaccaaaagt atttctccaa taattgtagt actatcccct gtttttggtg gtactggtgg
    32521 atttaccaaa gtatcatctt taactttatt atttttttct tctgacactg atggctttac
    32581 tacttctggt attggaataa tctgtttaac aggagtttct atcatctctt ctgatggtgg
    32641 tactggcgga ttcaatacct ctggtattgg cggatttatt atatcttctg atggtggtat
    32701 tggtggattt ataactatag tatcctctgt atcagatgaa cttgatggtt tccttggttt
    32761 atctgtgctt ggtggtactg gtggatttac tattggttca tctgtatttg gtggtgctgg
    32821 tggatttact ggtggatttt cttgtcttat gttttctata tttccaatat ccactattct
    32881 accaatatct ttattaccaa tttcaacctt aattttatcc ttcagttttt catatccact
    32941 tggagccatt atctccacta aataatatgt tcctggtttt tttatactag attgaattat
    33001 accatcttca tcactaacta aatccttaac tactatattt ccaaatgcat cttctaaatt
    33061 gaacttagca cctgaaagta catttttatc taaagcatcc acttttttag cttcaaaagc
    33121 ataaccttgc ttagtagtct ctactcctgg aggacttgaa ccattaatct gagccacatt
    33181 tcgtatagtc tccccaactt ttacatttgt gaagtctgca tcaaatgtaa cccaactttg
    33241 cacaggttcc ccttctttta tctgtttaaa ttcgtattta tttactaatc ttattgtatt
    33301 ttttccatct ccagcttctt ttttaagttc aagattatca gaatatttta tattagatat
    33361 ttctagagct gagtttactt catctaaaag atttacttgt cctgcatcat aaaccccata
    33421 agttgacata ttgattttgt atttaacaat ctggttatca ctactattta aaactttttt
    33481 atcaacttct ttttttgcgc ttattaatcc agaatttttc ttaaagttta tctcatcatt
    33541 tttatcaatt tcactattat ttgcatcaag aacagctcta tttatatagc tatcttcaat
    33601 actaattacc tttaatgtat aagctataaa aaactttgat tccacttctt ctttactgag
    33661 attaattgta aatccagtta taactttatc tacttgccca ctttcatttc ttttttcttc
    33721 tactttatat gaaatattat tggcaatttt ttctttactc caaggagtcc agctcccatt
    33781 cattggaatt tctacaactc taaaatcatg cccagcttct cctgaagtag ttaatctcat
    33841 gccttctggt atattatctg ttataacaac attacttatt ggtgatttat catcatttat
    33901 tgaaatctga tagtttatta cttgaccaac ttgagtaata tttgttgctc ctgaatgtcc
    33961 tttagtaaga agataatcat ctcctctatc aaatatactt acagtagcat catcttcttg
    34021 tatcttctta ccatcagatt caatcctagc tacattctta tatttattac catgttttgc
    34081 atttttgtca ctgattctag ccttatactt aacagtatac gatttatcta tatttccaag
    34141 attaactgaa atagaatttt tactctgttc aatattaaaa tcactaactt cttttccatc
    34201 acttgttact acacttacag aaccatttat caatgtcaat ccttcaggaa gctcatcata
    34261 caaaataata tcttttaact cttcctgctt ttcatttact tgtatactgt aatttatatc
    34321 tttattttta actggcataa atacagtagt tgattttttt ccttcaacaa gctttttaag
    34381 tgttccttta gcatctggaa catccgtttt atctccaggt tttatttcta catctatatc
    34441 tccacttcca ctttctattt ttccagtagt ttcaatatgt atttttgcat tagtatcttc
    34501 atcaaccttt ttaacatatg cttccaaacc tattttacct tgcattacac tttggccatt
    34561 aggtaattct ctaaatgtaa gtgtatactc tccattactt tctgttttat caaagcattc
    34621 acttgtataa tctaaattaa ctttattgaa tacatctggc accttaaaag ttattgaatc
    34681 tcctgcttgt attccttttg atgttggagt ataagttaaa ttaaatgata tttttttcat
    34741 tgattccgtt tctattgtat tgttattaat ttttacaaca aatccatagt cttcattttc
    34801 ttcattaagt tcttcttcta aaactttcaa taaattttca ttttctaata gattatcttc
    34861 ctctggagtt ttttctgtat ttttattgtc tagtggtttt tcatcttcta aagcttgttc
    34921 tgacttttta ttatctgttg acttatcttc ttctggtatc tgctctggct ttttattatc
    34981 tattggcttt tctccttctg gagtttgctc tggcttttta ttatctgttg acttatcttc
    35041 ttctggggtc tgttctggtt ttttattatc tgttggtttt tcttcctctg gggtctgttc
    35101 tggtttttta ttatctgttg ccttatcttt ctctggagtc tgttctggct ttttattgtc
    35161 gctttccttc tcctctccag ttgatgaatt agttgaacca ctttgaatct gactattctc
    35221 tcctaattcc gaagcataag atacagttgg atatattgta gacaccacca tacttaaaat
    35281 cataattaaa ctaattaata atgcctttct atttcctttc ttcatgtaaa tctcacccct
    35341 ttaggttacc tataatttat aatctaatct tccattaata tcgtatataa agtaattttt
    35401 actaaaaaat tatacaaaaa aagctatgaa aataaataaa tttatattca tagccggttg
    35461 caccactgac tcagtatatc cataaggtgc ccaataatag aatatacttc aacatctcta
    35521 taatatttca taaaaacaat taaaattgct ttatttatat taaaaataca attatatact
    35581 tctattatat tatgcctcta tactattgtc tatccttttt acatttttta ccttatgatg
    35641 agattaatta ataattaaat aaaatcctta tctacaacaa ttacacaatt atattcagga
    35701 tttatctcct taactaattt tgttatatct tcttttaagt catcttctga catgtttttg
    35761 gaaaattcgg aaggattaac tactatatca aatattaaat tcttcttgct tccttttcca
    35821 actattctaa aatcatgcat agattttaca agactatttt tcttaagtat ttcttcaagt
    35881 tcttttttgg ctgacataac ttctttatct tcaagacata ttgggtccat gtgaattact
    35941 agatatatat ctaatttttc agatatttct ctctctgcag tatctataat ttcatgtatt
    36001 tccattatat ctatgttgga tggaatttct gcatgtatag atgcaataca tctaccaact
    36061 ccatagttat gaataattaa atcatgtgta cctattatat atttataaga taacaatagc
    36121 tctataatag aatttaccat ttcttcatcc ggtgcttccc ctaaaagagg atttatagta
    36181 tcttttacaa gagagaaacc agcataaagt atcactaatg atactacgat accaacatat
    36241 ccatctatgg gaaaattagt aaatctagct acaataaatg atataactac acaacttgaa
    36301 gtaaatacat ctcctaaagc atcaagtgaa actgctttta aggctgaaga atcaatttta
    36361 tttcccataa atttattaaa acgactcaac cagattttaa tcattataga tgcaattaaa
    36421 agtataagtg gtattacttc aaattttaca ggaattggat ttactatcct ttctatagat
    36481 gactttacaa attgaagtcc aaccaacatc accataaatg ctactattaa tgctgataaa
    36541 tactctattc ttccatgacc aaaagggtgt tctttatcag ctggcttact tgctaattta
    36601 aaacctatca tagttattgc tgaagatgcc atatcagaaa gattattaaa tgcatcagcc
    36661 attatagcta tactagatgt gagcattcct atgaatacct ttatcacaaa taaaagtaaa
    36721 ttggataata tacctactac accagctaca taaccatact tatttctaac atctgtattt
    36781 tgaacgtttt cgctatctct gataaatgtt ttgactagga ttttggaaaa catgaaactt
    36841 acctccctgc cctttatttt attaacaaat tttataatct atatctttat ttatgataac
    36901 ataatttata tataaaattc ttaaaaatca ctctacttca gtaaaatatt attctatata
    36961 tctatttaga tacgaatttt atat
//
LOCUS       DAELTA010000017        36915 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.017, whole genome shotgun sequence.
ACCESSION   DAELTA010000017 DAELTA010000000
VERSION     DAELTA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.017"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(67..276)
                     /locus_tag="KPG13_000756"
     CDS             complement(67..276)
                     /locus_tag="KPG13_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984500.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(308..517)
                     /locus_tag="KPG13_000757"
     CDS             complement(308..517)
                     /locus_tag="KPG13_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984501.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            816..1235
                     /locus_tag="KPG13_000758"
     CDS             816..1235
                     /locus_tag="KPG13_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984502.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            1463..1918
                     /locus_tag="KPG13_000759"
     CDS             1463..1918
                     /locus_tag="KPG13_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984503.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2139..2399)
                     /locus_tag="KPG13_000760"
     CDS             complement(2139..2399)
                     /locus_tag="KPG13_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984504.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(2662..3015)
                     /locus_tag="KPG13_000761"
     CDS             complement(2662..3015)
                     /locus_tag="KPG13_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984505.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3032..8353)
                     /locus_tag="KPG13_000762"
     CDS             complement(3032..8353)
                     /locus_tag="KPG13_000762"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984506.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(8370..9353)
                     /locus_tag="KPG13_000763"
     CDS             complement(8370..9353)
                     /locus_tag="KPG13_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0984507.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(9354..10052)
                     /locus_tag="KPG13_000764"
     CDS             complement(9354..10052)
                     /locus_tag="KPG13_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG0984508.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10049..11101)
                     /locus_tag="KPG13_000765"
     CDS             complement(10049..11101)
                     /locus_tag="KPG13_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0984509.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11094..11522)
                     /locus_tag="KPG13_000766"
     CDS             complement(11094..11522)
                     /locus_tag="KPG13_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0984510.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(11522..11848)
                     /locus_tag="KPG13_000767"
     CDS             complement(11522..11848)
                     /locus_tag="KPG13_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG0984511.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(11864..13393)
                     /locus_tag="KPG13_000768"
     CDS             complement(11864..13393)
                     /locus_tag="KPG13_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0984512.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(13675..14097)
                     /locus_tag="KPG13_000769"
     CDS             complement(13675..14097)
                     /locus_tag="KPG13_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984513.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14097..16550)
                     /locus_tag="KPG13_000770"
     CDS             complement(14097..16550)
                     /locus_tag="KPG13_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984514.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(16550..16717)
                     /locus_tag="KPG13_000771"
     CDS             complement(16550..16717)
                     /locus_tag="KPG13_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984515.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(16744..17190)
                     /locus_tag="KPG13_000772"
     CDS             complement(16744..17190)
                     /locus_tag="KPG13_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0984516.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(17316..17744)
                     /locus_tag="KPG13_000773"
     CDS             complement(17316..17744)
                     /locus_tag="KPG13_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0984517.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(17758..18822)
                     /locus_tag="KPG13_000774"
     CDS             complement(17758..18822)
                     /locus_tag="KPG13_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG0984518.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(18827..19270)
                     /locus_tag="KPG13_000775"
     CDS             complement(18827..19270)
                     /locus_tag="KPG13_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984519.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(19519..19959)
                     /locus_tag="KPG13_000776"
     CDS             complement(19519..19959)
                     /locus_tag="KPG13_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984520.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20064..20261)
                     /locus_tag="KPG13_000777"
     CDS             complement(20064..20261)
                     /locus_tag="KPG13_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984521.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            20430..20750
                     /locus_tag="KPG13_000778"
     CDS             20430..20750
                     /locus_tag="KPG13_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0984522.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            20832..21338
                     /locus_tag="KPG13_000779"
     CDS             20832..21338
                     /locus_tag="KPG13_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984523.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22039..22794
                     /locus_tag="KPG13_000780"
     CDS             22039..22794
                     /locus_tag="KPG13_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0984524.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(22937..23365)
                     /locus_tag="KPG13_000781"
     CDS             complement(22937..23365)
                     /locus_tag="KPG13_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0984525.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(23786..23986)
                     /locus_tag="KPG13_000782"
     CDS             complement(23786..23986)
                     /locus_tag="KPG13_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0984526.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24169..25338)
                     /locus_tag="KPG13_000783"
     CDS             complement(24169..25338)
                     /locus_tag="KPG13_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG0984527.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            25574..26395
                     /locus_tag="KPG13_000784"
     CDS             25574..26395
                     /locus_tag="KPG13_000784"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG0984528.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(26471..27859)
                     /locus_tag="KPG13_000785"
     CDS             complement(26471..27859)
                     /locus_tag="KPG13_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG0984529.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(27994..28752)
                     /locus_tag="KPG13_000786"
     CDS             complement(27994..28752)
                     /locus_tag="KPG13_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0984530.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(28754..29470)
                     /locus_tag="KPG13_000787"
     CDS             complement(28754..29470)
                     /locus_tag="KPG13_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0984531.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(29472..30179)
                     /locus_tag="KPG13_000788"
     CDS             complement(29472..30179)
                     /locus_tag="KPG13_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0984532.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(30295..30831)
                     /locus_tag="KPG13_000789"
     CDS             complement(30295..30831)
                     /locus_tag="KPG13_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0984533.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(30933..32003)
                     /locus_tag="KPG13_000790"
     CDS             complement(30933..32003)
                     /locus_tag="KPG13_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG0984534.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(31996..33054)
                     /locus_tag="KPG13_000791"
     CDS             complement(31996..33054)
                     /locus_tag="KPG13_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG0984535.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(33041..33373)
                     /locus_tag="KPG13_000792"
     CDS             complement(33041..33373)
                     /locus_tag="KPG13_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0984536.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            33572..34693
                     /locus_tag="KPG13_000793"
     CDS             33572..34693
                     /locus_tag="KPG13_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG0984537.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(34821..36719)
                     /locus_tag="KPG13_000794"
     CDS             complement(34821..36719)
                     /locus_tag="KPG13_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG0984538.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        11927 a         6236 c         3950 g        14802 t
ORIGIN      
        1 gtttaagtac tttgaaaaat ataatacatc tctatgtata acttatattc ctaagaaatg
       61 cattactcat gtttttaatt cttccaacat ctttctatac acaattgtac atccttcata
      121 attcccctct ttaagaagga ttgaacatgt actagaatat ttagttaaag tatttaatct
      181 ttccatattt aagcttttta tattactgag tgctgtaata aaatcatcaa ttgctctaca
      241 acactctata accccatctg aatccaattt acacacaaga atattcacct actttatata
      301 ctatttatta atcatcttct gaatattcta tattaagtaa ttgataaatt gatgatttat
      361 attttttacc tgttcttctt ccatttaaaa tagctgacaa atagttttca ttaacaccta
      421 ttttcttcgc taattccctt tgagtcatat ttaattcaat aagtctttgc ttgacttgca
      481 ctcccaaaaa aggtatccat ttttttcgag catccattta tcaacttcct ttctaagtga
      541 tttatattta cttttatatt attttagctt acacaccccc tctatttagt atatattttt
      601 aatatgtaca catattaagt atataaattt atttaatact aaataagatt ttttgattac
      661 tttgtgctaa aattaggatg taaattcgta tcattaagtt aattataaag tcattttgac
      721 cttaagtcaa tgctttttat gtcattttga ccttaaattt gaaactattt acatcttaaa
      781 aagtcttttt gacattaatt gaatggagag tgtttatgga tactttagga gaaagaattg
      841 tttatttgag aaaagcaaaa aaccttaaac aatatgaact agaagaaatg ttgggctgtg
      901 acaacttaag taaatttgag agaaacatta gaaaaccaaa ttatgaaata ttaaaatcta
      961 tagctgagat atttaatgtt tcagtagact ggctattaaa tggagataat ctatcacata
     1021 aatcagattt aatttgcgat tcctcctcaa actatccact taatagcata aactctaatg
     1081 aaataaagct tttaaacaat tttagaaagt taaatgatta tgataaagcc aaaatagaag
     1141 gtatgattga attaaaactt catgaatatg aaaaagagaa ggatttagga aaaattgagt
     1201 ataacaaaaa taaagatgag aaaatagata aataattttt atgttgcttt tgatacgatt
     1261 ttacatcatt tctttgttct aatattttta tttcatttat tttctttttt ataattttat
     1321 gataaaataa ttagtatatt ttgctttact tgaatgtaat tatttataca aaagtcaaac
     1381 ataatataaa tgtagtaaat ttttagtttc aagtaatatc tcgttattat aactaaatat
     1441 ttattaaaat taggaggata aaatgggctt aagagacaaa ttcgcgcaat catttgctag
     1501 atcaaaaact atgagtggcc ctgagaaaaa ggctaatgaa ataatgggta aattgcttct
     1561 taagaaagct atattaccta tcgttttaat gttcgtgata ataatcgctg gagctatgct
     1621 taaaatcaat tcttgggtta cacttggcat aaatttagtt attgctgttg gtgctttctt
     1681 ctacataaga aattctagta aaaagtatca gaactttaaa ccttatgttg gcaatttgat
     1741 tagtttagag aaaaaaggta aaaaagagta cgttgctatt ataaaacaag gtaagttacc
     1801 agttaagtta caaatagctt atggtggtga agaccttgaa catgttaaga aaaatcaaat
     1861 ggttcaaata agttataatc cagatgctaa aatagctatt ttggttaata gacaataatt
     1921 taataaaaaa aaggagttgt cttaaaattg attttttcta atcaaattta agctcaactc
     1981 cttttttaat ttttatatat tatttttata tattattttt attacttttt ttaattaata
     2041 tttttaaata tcaaatcctt ctcaataaaa tagggtacta tctacaatac tactttatct
     2101 tttcctaaca atatataatc tggtgctact ctttcatgtt atacaccgtt ttgtaacata
     2161 ccttttatac cagtaacatc ttccttaatt tcctctacat ctgttttcat tatacccatt
     2221 tgaacaagta tatttttgtt tatttcttgt tgttgtgtag ataattctat aaaattttct
     2281 acagtttttt tatacatgtc tctatcttct tttttctcct gcatagtatt tttaaataat
     2341 agagcacata gtatacctat tgcccctaga ctactcaatt cggttattaa tttatccatt
     2401 atagcctcct attgtctaaa ttttttatac ttatagcttt aatttttgtt atattcttac
     2461 atcatgcaat attatatttt caggattatc aatttaatct tctgtacttt aaattgtagg
     2521 tgcaatttat agattgaata aataataaat atatatacaa tttctaatat aacaaaaaat
     2581 aagtacaagg cttacttatt aaagttaaga ccttgtactt tacataaaaa aaataactaa
     2641 tacatagtta ttctaaattt actattcaac tggtatttct aattctttgc atctaattct
     2701 aacttgctct ctaattactg ctggtacttc ttctattgtt tttttgcctc ttaaaattaa
     2761 agctacatat acttctacca ttccactctc acctccagat tttcttacac taaaagagct
     2821 tatattactt gcttcattga cagaactttg atttaacaac atctcatata tttctgcatt
     2881 tgctataaaa ttatttgtta tatcatcatc ttgctttact tgacctatac ttatgtctga
     2941 aatgtcttta tactgcattt ctacttttct accttgtttg atttgttcta ttttattttt
     3001 atcgaattta aacaattttc acacctccat tttaagcttg tggctcgttg ctttcagcat
     3061 catatctaaa aatcatagat ggacgtttta tagctgtcca tttatctcct ttttctacta
     3121 tgattttagg ctgagcactt tttatgttgg cttcttctaa ttcatagatt tcttggtaac
     3181 ttgagtctga ttttttgatt tcaactagtt tcatgtcttt gaataagtca gatttattta
     3241 tttttacttg aactttagtt ttatttatta attgatatat aggattgttt tccatcatgt
     3301 catcctttaa tccctcattt aatgttacta ttaaatcagt gttattttca gtaatgttta
     3361 tgattgaata tactttattg tttatcatta aaatagaatc cttttttact ttaagccctg
     3421 tttctgattt aaatataaac atacttgaat ataaatcgcc tgtaggacta ctggctttaa
     3481 tcagtatctt attttctcct aatactattt tattttgtgg tatctcaaat ataatgctat
     3541 ttatttccaa attagtctta gtagatatta attcatcatt tataaacatt tcaagttttt
     3601 ctataacatt attagggtct tgtactacaa ctctcacttg agatattgat ttttcaacta
     3661 tttctataga tgctttactt gtatctactt ttgttatcat gtatattgta tcatctgaaa
     3721 gctctatatc taaaatatca tttagatgtt caggaattgg tattttagta aattcatata
     3781 aatcagtaat tgatatagat attttataca tagatttatt tttaccagtt atccatagac
     3841 tattatcatt tttaattact agtatgtggt catcattatg tgacttaatt tctttaacat
     3901 tagttaatac tttagtaaaa gttcttatta tcttatcttt ttcactttct cctagtccaa
     3961 ataatccatt tgtattgtac ccttgagcgt acaactctcc ctcattagtt aaaacaagtg
     4021 tcattgagtt atatatactt atatctttaa cagaaggtaa tccagtaacc ttttgtaact
     4081 ctcttacatc attattgtgg ccaactccca attggtactc agtattcttt ccatatgacc
     4141 atagagtatt atctgtctta ataatatagg agtagttatc tccacaagca acttttttga
     4201 catctgatat atttacttca gtaagagtta ataattctat atcttcaagg gtttgattaa
     4261 tttctttata tgaattactt cctattccat ataacttgcc atccttagca acagccaatg
     4321 aatgtgtttt tgatatatca atatcagata cctcaaatgg caatattatt gaagtatact
     4381 taaaattatc tttaaatatg tttttataat ttatatactt attatttgtt tttatatact
     4441 tatcattaaa cattactagc ttttcatttt tcttattaaa aactatttta tctgatgtgt
     4501 tatctgtttt aaaatatgaa tgtagggaac cttcaagagc ttcattttta tcattatcat
     4561 atctatggtg aatacctaaa tgattatatc tattatctcc tgttgaagaa taaaattttc
     4621 catatatatc tttaataaat atagtattgt aactagaaaa tacagtatca acatctttct
     4681 ttatagatac tttataatat ggaactgaca tatccctata agattcatca gatataccat
     4741 aagtatctct acctgatgat atcaaattgt tattattatc tatataaagt gtattattag
     4801 ctgttacaaa aacatcttta acatcttcca cactatttgt attattatta tttgctatac
     4861 ttccatatcc actaagacct ccaacaaata gtttattgtc atttgttaaa atggctgaag
     4921 tatatccatt aactactatt ttttttgcat ttataatatt attaattact cgtataccct
     4981 ttatatcatt agtttctgtt gaaaagaata ccctctcttt tttccctgta gtataaataa
     5041 cattgtcatt tgttataaaa aatgtagctt cacttcctgc tgttacaaca gattttacat
     5101 tctcaatatt tatttttgta aatgtactta tacttccttc agtaaaccca aaatatccat
     5161 ttacaaatcc acatacataa catgtattat catttttaac aattattgat acatcattac
     5221 atatatacac atctctaact ccatttatag gtatacgtgt aaatatattc ctacttgtag
     5281 tatcacccaa tcctaactgt ccttttccat tattacctgc tccatataaa agaccatcat
     5341 tagttaataa tagagatgtg tttccatata cagctacata ttttacattt tcaacattta
     5401 ttttagtaaa tacatttcta tttacattat ctcctattcc taactgacca gaattattta
     5461 aaccaacagc ataacaggta ttatcatttt ttatcgcaaa tacatgttta ctattgtcag
     5521 taaatctatt tacatttata gattttatat tatcaatatt tattttagta aatacctttc
     5581 tatttatctc atctcccact cctaattgac ctacatcatt taaaccaaca ctgtaaagca
     5641 tattattatt agttaataaa tatgtagact tcgaatggct atatatttct tgtatattag
     5701 aagatatttc actactgcta ttctttttat tgtaatttat cttattattt aaatttaaac
     5761 tactattaac tgatatacca tgcgtaccct tagagttata tccacatata aataagctct
     5821 tatcagttaa tattgcaaat ctagttttat ctctacttaa atcataagga ggtataaata
     5881 tattttttat atttttcatt acctttttat aattagtggc attatatcca tcttgtgtat
     5941 ctgaaacatc tagttctttt gcactttcag atatattccc aaaaccatat aacgtgttag
     6001 aagataaata caatagagct ttatataaaa atatggcttt atcagaggta ataggacctg
     6061 aatttatatt agatgctgca atattattag atgattgatt gttaagataa ttccttccat
     6121 attgatgtat ataagatgaa cctccagaaa taagtaaatt atctggattt acatttttta
     6181 aattacttgt tgattctcca taatacaaat aggcaaaatt tgtagaatat gatgattgtg
     6241 tacaccttgt gggataatag ttatatatgt aattactaat atatccagca tgtgtaattc
     6301 cttgttgttg aactctataa gtagaatcac ttggctttgt ccataaagta ccatggtatc
     6361 cagttaaaaa agaaccataa tttgtacata atatagccat atattcatat ataataatat
     6421 tattaatttt agtaatatca tttgtacaag aatttgtact ataacttgct cctgaaggat
     6481 ttacataaaa ccagttattt attaatgtta aatttatatt gactccatca ttaggattaa
     6541 cacccaataa atagtttgta tttataccag ttgcatatac ctttttttcg tcagccacaa
     6601 taaatgttgc attatatgaa ctatatgcat ctgttacatt gctaacacca atctcagtat
     6661 attgacttgc tgatttatta ctaagtccat taacataact tgaactcttt ccagaagtat
     6721 atatttgctt attattagtt acaattaatg aaaaccagtt tgataaatca gtatttataa
     6781 caactttttt tacattatct atagatattt tcttaaatat atctgaatta gaaggaagaa
     6841 cacctgctat tgagttacta tttggcccag aataataaca agtgtcatca tttttaatgt
     6901 agaatattcc tccagaaata ttttttgatg ctactataga ctttacatta gagatactta
     6961 attttgtcaa ctcatttaat tgacttggta agtcagaacc cattcctttg tatctattat
     7021 ttcctagacc atatacatct ccattcccta aaataaccag agtacaataa ggactaaaaa
     7081 cgacatctct tacagaatgt atatctagtg gaagttcaat atcctttatt tccatttctt
     7141 cttttaataa agttaattta tcctcagatt taattaaatt tatataatta ctataactat
     7201 atttttcact atccattttc ttaaattcta caatgtgcat atcatcaaaa aacgaaccta
     7261 attgtccata agtattatct cctgtacaat atgaatcatt atttgaatca attagtatgc
     7321 tatgtgataa accaatgaaa atattcttag ctacaatatt ttttaaatct ataaatgaat
     7381 tatctatatt ctcagagtaa tttgctatac ctaactgata tatatcatta tttccagcaa
     7441 ccttcaaaat acctttatta tctataagca tagtattacc attaccacat gcaatagttt
     7501 ttatatttac ttcttctatc ttagtaaata gtgatgattg aagtgcattt ccaaaacatt
     7561 tttcctttcc acaaccatat atatcattat tatacgtcat taacacagtg tgttcagaac
     7621 cacaagctat agtcttaaca tcattaatag aaactaagct aaataaatct ctattattct
     7681 tatctcctaa acctagttga ccttgattat taaaaccagt agaataacat ttattgtcat
     7741 tctttattat aaaagctgat ttattatatg agaatacagc ctttacatta tcaacatcta
     7801 cttttataaa tgtatttaca tttgcagatg ctccattagt tcctagacct aattgaccat
     7861 acaaattatt tccagcaaca tataaagttc catcattgta taaaagatat gttgattgaa
     7921 gtccacatgc aatatccttt acgcctttta tttctggaac cttctcaaat gtggaaactg
     7981 tttttcctac tttttctaat cccaatccca tttgaccatc tgtatttata cctgcgacaa
     8041 gtaattctcc atctgaagta agtataactg catgagattc tccacatgca acttttttaa
     8101 tattagtaga tatatttggt attttcgtaa ataattttct tgttgtattg tcttctccca
     8161 atccaagttg aaagttacta ttatctccac atccatacaa gatattatta ttttttagac
     8221 caaatgtata gttacaacat ccagtaaaaa aattaataaa gtcataatta tcataccctt
     8281 catcaaattc atcaactacc atatttttat cgccaaagaa attacctctt tgaagtagtt
     8341 tagttctttt cattataaaa acctcctaat tatcttattt catacgaata tttatttaaa
     8401 gttatatctg tctggccaat tgaatatgcc ctattagatt ttgtttcaac aaagttatta
     8461 tccatttcat gtatatatct aactctattt gcattaaatg gtagaaccac attcttaaat
     8521 gtaacactac caagttctcc agttatacac tttctattta aatctaactt aaggccttct
     8581 atagctatta tgtttttagc attagttaag gtatcatacc aatatccagc ttcatctaca
     8641 tctatttctg actctaattc taagtatatt aaaatatcta atactttttc agataattta
     8701 ttgtaactat tttttaatac agatagttca gtttgtattt gacctcctat tacatcatca
     8761 atagatatat ttttaatttt ttctactacc tcatttatag ctcctgataa gtttttagct
     8821 tctgtagata agagttcttc tccaattttt acttttattt cttttatatt tttatcaacc
     8881 ttatcatcta aatcttgaat atccttttga gttacaaaaa ccaacgtcgg gtctacttct
     8941 aatgtaacat ttgaagtatt ggacaattgt aaaatcattt ttacaaccaa atctttaata
     9001 ctgccatctt cagcacgtgg cttatacgtc tctggatact tacctatagc tattatatta
     9061 ttctcattat ccagaacagc agcttctctt atcataaatc caccaactgt tcctggtaaa
     9121 atagtctcta taactatcca attaggattt ttttcatcaa ttcttagaga atttatattt
     9181 ccttcccaaa ctacattctt taaagcagtt tgttcctctg ttggctcata atattctcca
     9241 ttactatctc caacttgaaa cttggttaat ccaacaagct ctcctagtgc agttgcattt
     9301 gctatttttt ctctaccaat tttggttaat attatataaa atttttcttc caattatacc
     9361 acctccgatt taggataaac tactacattt tctacgcctg tatcatttgc aatagctata
     9421 ttaatcttac aattattttc aatgcattta atgcttaaag gataaacctt tatttcttct
     9481 ctacttaaca ttccagctcc acagtttaca acagcgctac attttataat tggttcaaag
     9541 tatggatgaa cttttacttc ttcactacat accatacctc caccacaata gaccatactt
     9601 ttatctagta ttattggttc taaatagtgt aataagtgag caggttttac tctttcaatc
     9661 atatcgctac aatctaaaag agttttatag tcacaattat ttgctataaa actcaatacg
     9721 aatgtaaatt catcactata aacttttata tctgtttctg attttgtata tgcctcacat
     9781 atacttttta taacttcaat agtactagta cctctgcttc tcattttagc ttttatattg
     9841 ctacgtcttg tgtcaaaatc aagttctttt ttttcaatgc agagtatgtc ttcccacata
     9901 tctaatcccc atgttgcagt gtcaacaaat aattggttaa aggtatcatc aatatcttct
     9961 ttaagaatat ttaattctgt atcgtatgca ccttgtatct cctctacaat gtaatttcta
    10021 tcaaatgatg gtagtttatc aattagcttc attgattttc cacctcacta aaattaacag
    10081 ttgttacact aggtatttta tcttcattaa tagttatatt atctgtactt ccatttataa
    10141 gtaggttact taaatcgtgt acaccagtag tatttataag tattcccatt acttttgtat
    10201 agattatctc tcctctaata tctctaaagt atgtatttat actttctagg aaagattctt
    10261 ttacattgtc taatgtatat ccatcttcta atttcattac tgcacttata tttatttcta
    10321 ttggtaatgg tgtcacaact gttatagttg gtccaatagg cttctcttca tctatatgtt
    10381 gctgacacct ttcaattgtt tctgtatcaa cagcttgatt atttttccca aatatcaaga
    10441 ccttaactgt tcccggacca tcccatcttg gataaacctt aacattatag actccatcta
    10501 cttccaaagc ccattcttca tagtgagctt tatttccact tgtagcttga tttctttgga
    10561 ttttataaaa tctttctttt agttcttcat ctgtctctat atctgtacca ccttcaaaac
    10621 ttcttgtgtt agtaatcttt gttactccag atatattatc aactagttta aattcacaat
    10681 ttgtaggtaa attatatttc ttaccaactt ccagagcttg aactgggcta ttgtcacctt
    10741 cttcactacc aatagttaca tcttttatta ctacaaatag taaatctcta tatgatatta
    10801 ttgtgccatt atttattaca gttcctttct ctccaataaa ttcaacttct ccatttgact
    10861 ctgtacctaa ctttctatat acaccaaatt cattgactct tttatcgaga aactggttat
    10921 atgtgtcttg tataaaagcc attttatgta tctttgaaag ttctagatat atctttgaaa
    10981 gttctagatt atttccagat accatgttgt ttagaaaaga accttctcct ttataaatat
    11041 caagattaat attttcaaga gttctatttt ttattacttc atatgtctgg tcactataca
    11101 tttatttcca cctccccata gatagtggat acttttacat ttgcactcaa tacgtctctg
    11161 ttaaagttag cactttttac gtttacttct aatatatatg gatttattag aagggcctct
    11221 tttatgaatc tactagcttc actttctgta agaccttttg tatatttttg tcctattagt
    11281 tctgaaagct ctgtcccata ttctaaagag tatatctcat gttcatatct atttgtcttt
    11341 atacacttgt atatccaaac ttttaaggct tctttttttt caattatttt aaagtcacct
    11401 ttttctaaaa taggttcatc tttttcaaaa tcccatgcca cttcacgaaa gattggcaat
    11461 tcttctgttt taggtaagat ataatcctct gggacaccta taaaaggaaa tattgtactc
    11521 attataagct caccaactta cttacaacag caaatttttc gcctattcta aacattatta
    11581 ccatatcccc agattcaaaa ttatctataa aaggattttt tacttcatgc tggtgttctt
    11641 gatttgtttc tgtgtcaaat gtttctagct gtctatcaat catccaacta tctatcaaaa
    11701 tatcttcttt ttctaatatg atgttatttg tctctatttt taaatctggt aatttacttt
    11761 taatttttcc aataaaaaaa gaaggttgat tatgatactt tccttcttct cttattattc
    11821 ctataaattc atttattgga ttagccatta tatcaccatc cttttataat actctcctag
    11881 ctgtattaaa atctcttcta ctacttaact tacttatttt aactacatca cctttttgtg
    11941 gagcatgtaa aaattctcca ttacctatat ataatccaac atggctgact ggattatgaa
    12001 aaaatactaa atctcctgct tgaagatttt tttgttctac tttcttgcct tttttagatt
    12061 gttgatttga cgttcttggc aaactaacat taactttttt aaaacaatag accattaaac
    12121 cagaacagtc aaaactactt ggtccatttc caccccattt ataaggttta cctagatgtt
    12181 tttttgcttc tgatattact attttagctt tatcagatac attaccagaa taaccaccaa
    12241 taataatttt tccttttctt cttccaaatt tactagcttc ttcaacattt ccaaataaaa
    12301 tatctatatg gtatgttcca ttcttttcta tagttattgc aggtcctacg tcatttactt
    12361 tatagactcc atcacgacca gaaactcctg taattacctg tatttcatct ccatagttta
    12421 ataaaggatg tttatttaaa aactcttttg tataataagt ttgttcatat ttaccaacca
    12481 taggagcagc gcaagttttt ttagatgggt caagtttttt ctttctacaa tctgtatctc
    12541 caccttcttc ttttctagga cagtaagctg taaattctgc tgtaaactct tttcctcctg
    12601 cataatcttc tccccctaag ttactttctt ccttaggttc atcctgtcct gctgactttt
    12661 catccataag attttgaaaa ttaagttcaa gctcaatttg atattctcca ttttgccaag
    12721 tatgtttgtc tgtatctata taaaatagtc ctacaagctt tgtataagaa tctttaactt
    12781 ttactcctct accagttata caacttacat ctccataacc tttaagagaa cagctttttt
    12841 ctatcccatt aaactcgcta tcaatatcta catcttgatt ttcttgttgc tgaattactt
    12901 tttgcattat tacatttact tccttaaaaa tttcattatc tattttttcg ctaatcttgc
    12961 ttccatactg gtctaccact attactttat tttttacatt ttccatgctc tccgaaaagg
    13021 tggtatttat aatattaaat ccctcttcaa acataacatt taatgtaaca gttccttttt
    13081 caataacatt aaacttatct aaattggcct ctatcatata ctttttcttt gtctttttac
    13141 ttgcttctgt atatgcactc attattgtgt cataaccatt tactcctata aacatctttg
    13201 tatacttgac attggtcttt gctattgttc caactgaaag cctattttca gcaaatactt
    13261 gctttgctat gtcttcaact aacttatctt taaaattgta tgacacttca ctttgtgtaa
    13321 gtaaaaatcc catatcttta gatacaaaac taatttcatt actgcttgaa tctttagacc
    13381 tattaattat cattcctcta aagagttctt tctcatctac atagaaacag actgtactag
    13441 ctattggtat atctatttgt tggaaattta catcaaatga tgattgtatt attgaaaact
    13501 ctagtgtcct tgatggagat ttataatcac ctgaccatga taccttgtct actatgtcag
    13561 ttatgtcata tatacttccg ttttttatgt gtacccaaat cttaataata aaaaatcacc
    13621 tgccttttca tttaattcta taagcagtta tttataactt attatctata atagttattg
    13681 ttttggagtt acttttggta tctttattct tttatactct tttaaatcta atgtaaaata
    13741 tacatctcga gtcccatctc tttcttcata actaaaccct tcaattatga cttccatatt
    13801 tatatttgtt tccgtaattg taaatcttaa tataaaacct tccttcatcc atttttctat
    13861 cttatttaca caatcatatg gtgatggaaa acctgtatag tcacagaaag tatagtcttt
    13921 atttggaaaa aaacttgata ttgaaatgct tttaagagct acaccaccaa aagttgctat
    13981 ttcacctatt ttgagtatac tagaagagtt tacagcagct tttccattaa tactaaagga
    14041 agatggaaat actggaaatc taaatctatc ttcagcttgt ctaagccaca tttccattat
    14101 aagacacccc ctaactgtgc caattggata cttgcaacta attttgatgt tatcttttct
    14161 atatcagctt cttctctaat cactattgta tcagctaact tagctatgtt tatatttcca
    14221 ccattttttc tgcttctata ttgatttgct tcttgtttag ttaaaaccat ttcaccttca
    14281 tggagtctgg tttgatagtc attataagga acatagttca atccaaatgc attattacct
    14341 ttcttacctg aataagcttt accagaatca ccagcctttg ttccagcata tggattatct
    14401 ttgtaatttt ttttactctt ttccactaga tttaaaactg ttcctgtgat gcttccattt
    14461 tttatcttat taatcatagt ttgccaccac ttgcatatat ctttaactat tttacatact
    14521 ttatctaata tgttaaaaaa agctcccaat attggctcta taattttcca tccagtttcc
    14581 aacagaggtc ctaaggtctc ccatacagtt ttccaaataa cacccagtgt ctctataaaa
    14641 ttgtttattt gaggtgcata ttcacctaaa aaagtaaaaa ttcttgtgat aataggacta
    14701 acagcttcaa ctacaggtcc gattagttgg aatgctgtag ttactattgt accaaatata
    14761 cttattaatc cttgtgcaat tggtgattta ttgtttatac tatctagtaa attatttact
    14821 aagtttacta ctggaagtaa tgaatctatt aatgctgtaa aatcgatatt ttgtaatagt
    14881 gtacttcctg cgttaacaat tgcttttggt gcattcccta tcattgttgc tgcatgattt
    14941 tcaaaacgtt cttgcttatt ttcaaccatc attttacgct tttcagcctc attagaaatt
    15001 cgatattgag tagaatcaga aaactctttt ccatttttta cacttctaga ttgcatctgg
    15061 tcatatttgt tgccaataat tatcttacca ttcattacat tgcctatttt accagccatt
    15121 ttttcagata agccagctaa attgcctaat ccactttcta taaaagtttt tattttcttt
    15181 agtccttgac ctaaaatatc atcaaaaggt ttaacaatac tcttcaagcc attgttaatg
    15241 cttgtttgag ctcctgatat tatacctggt attgtattac tcatttcttt agctaacccc
    15301 atctgcttta cacttatgcc ttccaatgct tcatcaaaag ttttataatt ttctcctacc
    15361 agagtattaa gagattccat gtttcctttt gctgcactta agaaagctgc tgatacatct
    15421 tgttcactac ttgtattcat atttaaagct ctaacatcta ccatagcttg agttatatct
    15481 ttttcttttt tgccaccagt aatattacta atcattttag ctttagttcc cattgcaaca
    15541 acatcttcag ggtcaaaagg cgtatcattt gccattctta ctaaactgcc ataataatca
    15601 tcaacatcta tttgattaac ttttccagta tttcttttca ttacactttc tagaacaact
    15661 ttctgatttt gttcctgacc aagtaaatca acaccctttt tcacagcgac acctatagta
    15721 gcaccatttg tcaaatcaga tatcttccct tttatcgaac caagtattcc acttgccata
    15781 tcctttacag ctattgcacc actccatacc ttacctgcaa ataatctcaa tttgggaaat
    15841 actttactta taatgccact tgccatatct tttatagcta ttacaccact ccaaaaagta
    15901 tttttaaaca acattaactt aggcaaaatt ctacccatag cactactcac catatcttta
    15961 agattaatac ttccttgata aggacgtttc tctacttgtt ttaattttcc caagatacta
    16021 cttataactc tcttagtcat atcttttgct tttattacga tattccaaac cttgccacca
    16081 atctttttta aatttccaag taatttattt ttagtttttt ctacttcatc acttttagct
    16141 ttaaatatta atataatcat aatatttgaa ttaaactcat ttttcttttg aatcaactta
    16201 ttaaaataat caataatcat agctatagtt attgctagta tcataatact tccatttaaa
    16261 cttccaataa ctcctgaaac agcatttatg ctagaactta attttgtcat agttgaactt
    16321 acactactag cagtagtttg aacttttctt aacgaggaat ttaaactgtt tactctatta
    16381 cttgcacttc caatagaatt ttttatgcta ctagccatta cattagtgga aagtgaaaca
    16441 gccacagttg ctctcctaaa attatcagtt actgatataa tttgattcat ttttggtgtg
    16501 taattatctg taaggtacat atttatacct tcttctctca catttcccat tacataccac
    16561 ctcctgtcaa aggacacact ccatatccat gtttcatttt ttcaacttct tcctcaattt
    16621 ctaaggcgaa aaaagcttca ataagcttta attcgccttt atccattgca taaaaaaggg
    16681 acggtcttat accttttttc ttccaataat agtacattat tgtagtcctg gtatccgtcc
    16741 ctattagttt tttacctctt ctatcactgc atttttacca taacccataa catcttgaag
    16801 tattctatat aagttggtaa tttcacctgg taataataat tttcttgcta attcttttgg
    16861 tgttggagcc ttaaatttat ccattagctc cttattttta aataaaagct ttccatccaa
    16921 atcacaaaca ccttctataa ttgtaagcat ctgcatctcc ggtaagtcta tatccgtttt
    16981 catattttca tcaattttaa gacaagtatt ttgtatttct tcatctttac ttattgttaa
    17041 agctctacac attactttaa attcctgccc aaatatagtt gataatctct taagttcaac
    17101 tatagtactc ggtctttcta agttttctgt atctgaattt aaaagtaaat ctactatgtt
    17161 tatatttttt gataatccat tttcactcat tattatttgc tcctttatca atcaaattca
    17221 atcaactaac ttttttactt attaactatc tgaatttatt aagtatatat ttactaaata
    17281 tcagtataaa accaaatata aaactaaata taaaactaaa ttatatcaag aaaatcatac
    17341 tcagtaaatg tgaaaggtgc ttctatctct ccttttacgc caacctccca atcagctaaa
    17401 gttaaatcat caaatgctat attctttact gctattcttt ctgctccata agaatctggg
    17461 tcatttaatt tagatatagc aacaaaccta ggttcagaac ctctctttat cttttctcca
    17521 attaacttgt gcattcttga actaacgtgg tatagagtta ttgagccttt tcctttatat
    17581 cctatatact ttgtatcagt acccatttga cctgctatta taatatcttc ttttgtaaat
    17641 tccatctttg cttgaaattt ctttacttct gctactttgt ttccatcaag ccaaagttct
    17701 ccccaagtac cactcattac atttctagct tccatatttg ccatattaat aatcctccta
    17761 tatttctatt gataaatcta tatcttccat agcatcaagt acttttattt ttgcttttaa
    17821 aaatacttta gaacctgtgt tagcttcttt tatttcttgt aatgtcatat aagataaatc
    17881 tactcctttt gattttaaat acgatttttg tgcttcaaaa tctatttcaa cagtagaatc
    17941 agattctata agtgctgatt tttctaattc ttctaaataa ctttttatag ctactatcaa
    18001 taaacatttg ttgtcataac tgttagtaac ctttcctata tagtcatcta ttatcacctt
    18061 tcttatgtca ctatgtataa tatctaaagt gtcaactatt tttattttct ggaacatttc
    18121 tcctttttct gctgttaatt cagttaaaga atttactcct ctagcaattc ttatagctcc
    18181 tgcttcttta ataagtataa gctctccttt attaaccttt gattctgcat caactttcgt
    18241 catcttaggt atatcgacta catcactaag tttagtataa gttactgatt gacttaaagg
    18301 tgtacctgct ataagtccag ccaccctact tgtaaactca tcaacactgt atttcttttc
    18361 tccaactaac acatcttctg tagtaaaatt aattatccct tcatgatttc ctacaacttt
    18421 tcctaataca gctttaacct taaccttatc tatatctcta agtttaatta tccaattttt
    18481 tatagcagtc ttatcagctt ctactgcttt tggcatacat agataattaa attccttagt
    18541 ctctaaaaaa tctaatgcag tttgaatatc tgcttctcct tctattacat aaactaataa
    18601 cttatttgga gtgttaacat ttcccatcaa agctaaattt atatattctt tattttcagc
    18661 agataaatca actggtatat cctctttttc atgtatttca ttaagaccta gtgccttagc
    18721 atctttaagt accattgcaa ttattcctct agctgaacgt tctttaacag ttgtagctag
    18781 ctccttaaat gatatgttga tacttggtaa tcctatagcc atactatcac tctcctaaat
    18841 ttaaattgat ttcttccata ttttcatata ctacatcttc ttcatataca ctatccagat
    18901 agtttagtgt aaatgtaaag ttcaatacat aaattgactc ttctttttta atactttgtt
    18961 ctagctttgt tatatttaat tttctgtctg ttacctttat atttctgttg aataacttat
    19021 ttaattcatc tgaaacttca tacattctaa tactctttga ctgtggtttt ggtaagtatt
    19081 ttatagagat aacaatatct ttgttattcg tatttaacat tacaggtgta caaataactg
    19141 atagtatact tacaaaaaaa caagaccctt cagagccttg cacactttca tcatcaataa
    19201 aaatactttc agtaaagttt tttttgagaa tctcaataat tgtttctaat atttccttgt
    19261 atttaagcaa ataatcacca cactatcact aaatattctt gcttaatctg ataatcttac
    19321 tataaatgat ataagtcatt tttaaatata tatctcattt attcctgtca agtatcttct
    19381 atatttttag gtaatattat tcttttcaat ctatcacctc cttcaatatg gatttctttc
    19441 acaataacat tatctcacat attttattgc ctattgttgt aatggtgtat ataaagtgtt
    19501 gtaaaattgt cctatttttt ataatatctc actcaattta aatatcatct ttttctttaa
    19561 attgtagcaa gatgttctat ctaagtgtag tttcatagaa atatatgtca tattgttttt
    19621 tgtcttacta ttataaaaaa gattaaaaaa ttccatttct atatcattta gacacgttag
    19681 agcattttct atcttctctt tttctatttc tatatcttct ttttccattt ttaattttct
    19741 taatctttcc tctcttttta acacctcatt ttccacagaa gagcttatgt tatatgtgtt
    19801 actagtcttt tctgtatata ccattgctcc acatccagag tattcattct cacatctttt
    19861 tatgtccaag ttaatattct ttatctttat atccaaatac ttataattat gtaatctttt
    19921 atctgtttct ttaaataata tatctttttt gttttccata gtacttcccc ttttttatat
    19981 acttattatt agcttcaaat ttacaataat ctctttagtg cacagttttt tactaatctc
    20041 acaaccttca agaacatatt gttctataac tgtataaaaa aaacttcttc tatattttgt
    20101 tctaaaatac tagaaatctt cttagccata agaagtccag gattttttat gcctctttct
    20161 atttgagata tataatcatt tgtaattcca gcctttctgg caagttgaat ttgtgtcata
    20221 cctttgcatt ttctataata tttcaagtta tttttaaaca ttgttttata cctccattac
    20281 tattaagaat attttgttct tcctcactta gattatatag aattatttgt tctttatcaa
    20341 tagattttag aaaaaaatat tcttttatat tttattagtt ttatttttta gaacaatttg
    20401 ttataatcta tacataaggt ggtgttataa tgtttgccaa aagattaaga gaacttagaa
    20461 aggaatttgg attgactcaa agagaacttg gagaaaaagt aggcgtttcc caaagagtct
    20521 tagggtacta tgaaacagaa aacagatttc ctgatgaaca cattttaaat aaattggctg
    20581 atgtatttaa tgtatcagtt gattaccttc ttggaagaac cttagttaag gaaaatattg
    20641 atacagtagc tgcacataga aaaaatccac atgaagagtt acctgaagaa gcacaagaac
    20701 aacttaatga ttatatagaa tttttactaa ataaatataa aaaaaaataa accatatgat
    20761 gagcagtcta tttttctgct cttttaatat aattcaaagg caaacataca ttctatttta
    20821 gggggatttt tatgaataat ttggataagc tttttgaatt agcttctcaa gaagagataa
    20881 taattcatta taccacttat attgcaggtg atttagaagg actatatata aacaaacatg
    20941 gtattaaaat tatatcatta ctcagtaatt taaaacaaaa ctcaaaaaaa ttgacatcca
    21001 tcttagctga agaacttgga catcatttca ctagccttgg atactatgta tcctcttata
    21061 acgattatta tacaaaaatt attatagata agtgcgaaaa taaagcctta aaatgggctt
    21121 gtgagttctt aattactgaa gaagatataa taaacataat taactctggc atcacttgcg
    21181 tttatgaaat ggcagacata cttaatgtgg atattacttt ctttcaaaaa agattagaat
    21241 ttctatcatt aaaaaaacag tctttacaac ttggaaataa taaatattta atattaacta
    21301 atttgccgta tttctacata tttgatccaa tttcataata aaaatagtag taattttaac
    21361 tggcttaaca gtaaaatcta tatatttatt ttaacaaatt ttggtttcta taaattcaat
    21421 attaatatat aataatgata tcatattatt taaaattcta aaaacaaata tatgcttttg
    21481 ttaagaaagg ttgtttgatg aatgtataaa gaaaagttgt ttgataatta ttttaagttc
    21541 ttagcactat tattttggcc aattatgtgg tataagtgga tagtaatttc aaatggtact
    21601 cttgaaaata tgttatttac catatatgca atcgtcgcaa ttgttttcat cattttatat
    21661 tcggtgttta tgataaaata taaagacatt acacaaatag atttctttta caggataagt
    21721 actctacttg cttttatatt tacacttttt agctttttaa tatacccaaa gagtttattt
    21781 ttcttgtatt taaagattat ttttactgga atataccttt attattcaat agtaaaaact
    21841 cttaaattta aagatgacga aggtgtagtg ggtattatga gctcactttt attaattgta
    21901 ataactttgt tctactaaat ttaattctat ttgacttaat atggctttaa atattagaca
    21961 tctacattat tttctgatat tatttaagat ggtagtctta tattaataat gaaatacact
    22021 atgaaggagt taactattat ggaaaaaaaa gttttagcaa cagttggaga aaaagagata
    22081 actaatattg atattgaaaa tgcattaaaa tcattagacc cttatcaagc tatgcaattt
    22141 aaaactgaag aaggaaaaaa acacttatta aatgatttag tgaatcaaga attattcttc
    22201 ttagatgcta aagaagaaaa attagatgaa gaagaaatat ttaaattaga aatgaaaaag
    22261 atagaagaaa atgtattaaa gcaatttgct ataaataaag ttttatctag tgttaatgta
    22321 acagaagatg aaaaagtaaa attctttgaa gctaataaga gctctttctc taaaccagaa
    22381 agtgcaactg ctaaacatat attagtagat agtgatgaaa aagctaaaga aatacttgct
    22441 caaataaaat cagaagaaat atcttttgaa gatgcagctc ttaaacattc ttcttgccca
    22501 tctaaagata tgggtggtga tttaggaaca ttcggtagag gacaaatggt tcctgaattt
    22561 gaagaagctg tgttctctat ggctaaaggt gaagtaagtg aaccagttaa aactcaattt
    22621 ggataccata taataaaatt agaagacctt caagaaagta cagagtctac atttgatgaa
    22681 gttaaggctg aagttgaaaa aagtttatta tatcaaaaac aaaatgaagt ttatggaaat
    22741 aagataaatg ctcttaatgc taagtacgga aaccttgtaa aatacaacga ttaatcataa
    22801 ggtcatatat aaaaaagctg tcttttcaca atatttatta gaaaagacag ctttttatat
    22861 taaagtttaa atattcagtt tcatattatc atttttttat tgtaaagaac tcagcttaaa
    22921 agtttaaata agatttttaa ttatcattag ctaaattttt atatccacgt attatctcat
    22981 aacagtaacc tattacatct cttcttttta ttatcccaat aaatgtgtct tggtcatcta
    23041 taacaggaat gaaattttga tttaaagatt ttataactaa atcttcaata tctgcgttga
    23101 tggaaacagg agaattatcc atttttctat ttatatctgt cactggaaca tcttccaatc
    23161 cctttaagtc cagatttaaa tcatttttta atgtccataa aaaatcacct tctgttatag
    23221 ttccaacata tttaccatcc ttacttatta ttggaattgc agagtatttg tgatattcca
    23281 ttttttctag ggcttgcctt atagtgtagt cttcatatat atatgctact tcactttttg
    23341 gtgttagaaa gaataatatg ttcattacac tacccaccgt tactgaaaac ttataaatat
    23401 tactcaatat ttataatgtt acttttttgc agtaactttc tcctttctta ttttctaagt
    23461 attcttaata ctatacgctg taacaagcgt ttttcctttg ctcattttaa tcttattatg
    23521 ctttattaat atttttatat ggtctataag tatcacttat tctcttttta gaaaatttac
    23581 taaaaattaa ttgacttaaa cttagccata ataattttac tctacactat taattttaac
    23641 atatttatct cattattaat gattttattt taataatttt aatttatttt gctattaatt
    23701 ttatttacaa aaactgaaaa aaccgctaaa tgattagcgg cttatctctc atattactag
    23761 aacactaata tatatgaatt tttaattata atctaactac gttagcagct tgaggacctt
    23821 tagctccatc tactacttcg aactcaacgc tttgtccttc ttctaatgtt ttgtatccat
    23881 ctccagttat agctgagaaa tgtacgaaaa catctccttc accttctaca gatatgaatc
    23941 caaatccttt ttcgttgtta aaccatttaa ctacaccagt tttcattaat aaatcctcct
    24001 aaaaaaacac aaatatttag ctactctttt ctgtaacctt attactactc tatcatatat
    24061 agctatgttt gtcaattatt attattttta tacattaata atcaattttt ttactttata
    24121 attttaaatg attattatct tttacacttt atttttcttt ataatttttt aataatttat
    24181 ttttattata tggaatctta tggacttatc actccagcct aaatcccatg gcaccaacat
    24241 tctatcagta ttatggcaag ttaccaaagg atatcctctt gagtctaatc ctgtcactgt
    24301 cgaaacatgt gttattctgc ctttcttctc ataaccaaca aaatcccctg gacgtaaatt
    24361 ataagcttct ttgtatactt cttcatagga acctttagca attaatgaac ctcttccact
    24421 atacagtgca taatttttaa aagcttgtgc atttacccat gcttttgtac ctgctctctc
    24481 gctataattc caaattgagt tctttttgaa ccttccactt tcaaacatta tctgtgatgc
    24541 aaagtttgca caatctccac cttctggatt ataatcccta tagtttggat taaatttcat
    24601 accatgttct tcatcagctg cggctccaca gtatttatgt gcataatcta tagctttttt
    24661 ttgttcatct gtaagctgta actctacacc atcttgttga ccaatataat ttcttatatc
    24721 atcagatttt atattttcta aatttaaaga gtctgcaaaa gggtctgtat accactcttt
    24781 agttatgaca tattgattat ctataatttt tacatttaga taatgatatg tacctattct
    24841 aaaggcattt tctttgtcct tttgattttc atatgaatac ttatattcag tggatactga
    24901 acagattata ccatagaggt ctttttcttt ttcctttatt tttttaatct taacccttgt
    24961 ctttatgtct ttaaatttta caccttgttt actagaccag ttttctaaat atttcatctt
    25021 ttttacttca ttttcataag cccacagacc aaatttttta tccgtttcat aaagaccttt
    25081 taaaatattt tcatttttct ctaatattgc cttatttctg tagtcaaata agttttcaag
    25141 caatagttta tattggtctt ttacatcatc accttcaaca tttgcagatt ccatgacttt
    25201 ttcttccata tttttcatac ttaaatcatt gattaaaaat acacctgctg tagtagctaa
    25261 agacaaaaat acaaatgcca ttataccttt gatgtactta ttcttatttt tcagtatttt
    25321 caaagctaca cctcccaaat agtacacctt acaaatcaaa ttacaaagaa tattctgtca
    25381 catatagtaa ttttcatatt taataattct ctattttatt atgtccaata ttaaaaatta
    25441 attgtatcaa tacactgttt tttttacaat tttttatttt ataatctact tttttctttt
    25501 ttttatagta atatatatgt atataaatat tttggtatca ttataattaa tacatactaa
    25561 tggagggttt tttatgcaaa aaaaagtcgc agcaattaat gacttatctg gaataggaaa
    25621 atgctcatta tcggttgcta tacctatact ttcagcatta aaggttcaat gttgtccatt
    25681 tccaacggcc atactttcaa gtcaaactgg ttatcctgaa tttactttct tagactttac
    25741 tgatgaaatg gtaaaatatt caaatgtttg gaaaaattta aaagtcaatt ttgatagtat
    25801 atatagtggt tttttgggtt caaagcatca aattgagata gtggctaatt ttataaacga
    25861 ttatcccaat gcttttatcg tagttgaccc tgtgatgggt gataatggag ttatgtatcc
    25921 aatattcaca gaagaaatga gacaggaaat taaagaatta gttaaacatt cagatttaac
    25981 tactcctaat ttaacagaag cttgtttctt aactggaaat gattatacca aaagcgatta
    26041 taatagagat gagcttatat atattgcaaa atctgtatct gatttgggac ctagcaaagt
    26101 cgttattact ggtatattag aagatgataa tatattaaat ttggcttaca atagagataa
    26161 tgaccatgta ttctttacat cagtcaaata taataattgt tcttacagtg gaactggaga
    26221 tatattcaca tctatacttt gtggtatgtt agtaaataaa catgatttgg gagtagcggt
    26281 taatactgca acagatttta tatataaaac tataaattac acatctcaat ttgatactga
    26341 tagaaatgat ggagttatgt ttgaaaactt tttatcagat ttaaccaata tataaaatca
    26401 aaaaaactat ccattttata aatacctata aatcataaaa tttaggtatt tacaaaatgg
    26461 atattattta ctaaactatt ggtatttcaa ttgttaccat agcaccacca gttttcacag
    26521 aattttcaat acataaagtc ccattatgtt tttgtacaat attatttaca atatagagtc
    26581 ccattccata atgagattta gaatttctac tcaaatctcc tgtataaaac tgttctgtag
    26641 cttttgacaa atctgcttta gaaaaaccac ttccacaatc tgtaactaca aattttaagt
    26701 atttattatg tactgataca cttatgtata gtttactctg gcttggtgaa taatctatag
    26761 catttgaaat tatattcata atagctctga ataacagttc tctatctata gttatatttt
    26821 taggcaaatt atcttgttta taaaactcca catctaagtt tttagtacta gctaatgctt
    26881 ctatttgcac taaaacatct tttataaact cactcatact gattgcttct ttatttaata
    26941 tatagccatt ctctgttttt gatatatcta ttaaagtatt aatatatttt tctatttgag
    27001 tgacaccctt tgagatatat tccatgtatt ctaataactc aggattttca tttatttcaa
    27061 ttaataagtc agtatttcca tgtataattg ttagtggtgt ttttatatca tgtgccagtg
    27121 cagatatctg ttcctttcta ttttcttcta gcatccattg tttttctaaa gaatctttta
    27181 acgccaactt cattttatcc atagaaaaaa ggacattatt aatttcataa attttactag
    27241 attctacaac aaaatctaaa tcctgttgtt ctattttttc tgtagtattt tttaaaagct
    27301 ccatttcaat attaaatttc ttgccaaata cttttgataa tataacaatt tcaataaaaa
    27361 atattatact aaaaataatc atacccaatg tctctggttt tggtaaatat gctcttaaaa
    27421 ttggagaact atactctgat actattttgt attttattat aaaaacttca tctttcttaa
    27481 aaaactttat ataactttct gaatatgcat gcctttcttc aatatctctc attaaattcc
    27541 atacttcttt tgattctttt ttattaaagg tccctgaaat taaattccca tccaaagtat
    27601 aaaccccata ctcatatata cttggaatta tatcttcagt tactttttca cttgaaataa
    27661 tctgttcttt atatttatta aattgttttt ctgcataatt agctggtaaa attacacctg
    27721 atgataataa tacagaaaat gataacatta aaaggagtac aattgaaatt gtcataataa
    27781 agaataaaga taaatatttt ataaaaatag ttcttaactt ttcaactttt ttgttgttag
    27841 attttttacc tccaagcatt tgtaccctgt cctccaatag tttaattaat tcataattaa
    27901 tacccaacca gttttttatt tgattatttt atatcttttt attgctaagt tatattttaa
    27961 cataataata taaataaaca gtcaattttt actctattct tctgattttt taccttccca
    28021 tttagaaaac caaatacata aaattacaaa tgctaaaatt gttacaaaac cacaaattaa
    28081 acctgttgat tgatatgctt ttattgattc aacctttata tattcaaccg ttttagaact
    28141 tgaaaaagac gaccaaatag ttataaatct aacaccccat gcacaaggaa aatactgcca
    28201 aataccagaa cccaatccag ttaataataa tgctgaaagt aacatttcaa ctatacctac
    28261 tccaattgat gttccttttc cgaaccttaa acttaagaat aaatgtaata tgtattcaaa
    28321 taaattacac ccaattaaaa ttagaattga aatcatataa aagcttgatg taaagtaagc
    28381 cttagatata ccaaatttaa aacctaatac agacactaat gttgctacaa aaccaagaca
    28441 tactaagatt atatatttac ttataagagt aagatatttt ggattagatg ttgttaacat
    28501 atgtttataa ttccctgcat aatattcttg ttcaacaaac attgaacaca caacacttga
    28561 caataaagga aaagctattg ctataacttg taaatatgct actatcttta gtatattatc
    28621 agctcctgaa accttataat accataaaaa caatcccatc cccattaaag caatgcaaat
    28681 gtgcattagt attataaaat ttctctttaa ttttattaaa tcagaacaaa atattcttat
    28741 taactgtctc atattacttc acctctctat tattgaacca aacccttgta gctaaagcaa
    28801 ataatacaaa taaaacaata gatattaata ctcctggcaa aataacagca ttatttaaaa
    28861 attcacttcc ctctggtaca ggaagaccat ttggcagaat atgcaataca acagccatta
    28921 atcttgaagg aatcgaatat ggaaacatat accacatttc tgtagttgag aaaacagcaa
    28981 ctccaacaat agtaaagaac atatttgtta gaactgtaac aaacatacca aattttgaag
    29041 ctaaaaacat acaaagagga atctgccaca taaatagtaa aataattaat atacttgcaa
    29101 tgatattttg acctattgaa atagtttctt tatagattat cccaacaaaa actacaccta
    29161 ttgcagaaat aattgatgct ataaaacaac aaataataca gtaaatcatt tttccagtcc
    29221 aaacgtcttt catttcaatt ggcaatggta aaataccacg atatttactt ttttcatctt
    29281 ttaatactat taaagaagat attaatgtta atgctcctgg aagaaatgtc gtataccacc
    29341 agttgaaagc agcattttgc cctccaccta gtccacacaa aagtatacaa aacactggaa
    29401 ctatccatat caacttcatt ccaaaagttc ctttcatctt ttgaaattcc actctaataa
    29461 atctttccat attactgccc tctctttcta ttttcagcta caactttcat aaaaagttct
    29521 tctaagtttt gttcttttga tacttctcct tgataaccca ttactccatt agaaattatt
    29581 cctatttcat ctacaacttg ttcaacttca gacaagatat ggcttgataa aataactgta
    29641 attccttgtt ttggaaaaga acgaatcaat tctctaagtt cttcaatacc aatagggtca
    29701 agaccatttg taggctcatc taagattaaa agttttgggt gatttaataa tgcaatagca
    29761 ataccaaggc gttgcttcat gcctagagag aattgacctg aacgcttttt aaaggtatct
    29821 cttaaatcta cagtctctaa aacttcttca atgcgagagt caggaagacc aagtaaagta
    29881 gtatggactt ttaaattttc tcgtgctgtt aaattctcat atatagcagg agactcaata
    29941 agagaaccaa tatttgataa atcctttcta gtccatctat gtccctcaaa cattattttt
    30001 cctgaagttg gatttaaaat cccagttatc atttttaata aagtagattt cccagctcca
    30061 tttggtccta ataatccata tatagaatta gatttaattg ctagtgaaat gttatcaact
    30121 gccatctgct ccttaaattt tttagaaact ccttttacct gcaaaatata attactcata
    30181 attttcatcc tttcttttgt caaacttatt tacaagaatc attctaaatc ataaatataa
    30241 ggattttata agaattaaaa aacaaaaaaa gatgtgttaa aaacacatct ttttttatct
    30301 taattctatt aattttccat tgtagtatat ctctacttct tcaatccaaa tgctattatc
    30361 ttcttcttgg atgacatttt taataatatt atttatagta tatattatat tttgttcatt
    30421 caaataatca tctttccaag aaactctact ttttttatct ggttctatca agtttatttt
    30481 aacaagatta tctttttcct taacttctac ttccatgtct aaaccattaa aatactcatt
    30541 tgataatgta tctaagatta atcgcagttt atccttaagt gaactttctt catcaatctg
    30601 taagtacttt actattttct catcctttga aattatctct ataggtattt tagtagtttt
    30661 tttagaatta atcattttac tagttgtttc ttcttttgca tcttcatcct tctttacaac
    30721 tgctgattct ttagaatcaa ctagctcaac tgcactgatg ctataatcca tacatgccgt
    30781 actcaataaa gtagtagcaa ttattaaaaa acttactgct attttattca taagtatccc
    30841 tctcctaatt ctctttcgat acttattata ccatagtata gattatattt ttatactttt
    30901 aaatcaattt ataaattatt atagtaaatt atttacaatt ctaagctatt tgaatttatt
    30961 tttatagtat attctttctc taaattcgac agatttttat tcaaaagccc ttttatttcc
    31021 ctcgtatttc tatacttgtt gctatgttct tcattatgtt cataatcata tgaatcattg
    31081 tcatattctt catcattatt aaattcttca attgcttctt ttgactttat atcacaatta
    31141 aatttatact tatttactgg tatatcaagt cttaccttag aatttggaac ttctatgtcc
    31201 aggaacttag caattggcat atcagagtac aagtaaaatt cactttcata atttgaattt
    31261 ctacctattt tctcttttat atttacattt tcaggtatat actcttctat attattgata
    31321 aatacatctc cattctctga agatatatcc aatgactctg attctatgtt tacattttta
    31381 acgcctaaaa ttgactttgt tgatagatga agattgctat tagatattaa gtttaagttt
    31441 tctaatctat ttactttatt ttctgttata gctctgctaa aacctccaga agaagtttta
    31501 tatgttatat tatttgaatc tacatcatca gatataaata agtacgaact tatacttgat
    31561 gcatttatat ctacattatt tggtacatat attctaattt ctctataatc acgtgaaaac
    31621 atactattta tagatgtatt taataagtgg tcgaaacttc taatcttttt aattttattt
    31681 tcagattttc tttcgccttt tacaactaaa gtcttatctt gtagggttac actgtatttg
    31741 taaaattcat tattccctct tgttgtcaca cgcacatctt catttgaact ttttcttatc
    31801 ataacattta tattgtcaac agctatatct aacttgtcaa tatttacttt ttcttgataa
    31861 agtttgtttt ctttgtttaa ttcagcttcc ttttctaaac caaagtttat taggcttggc
    31921 ataactaaaa tccctgacca tgttgtccct attataccta ttaccaataa aactgcagca
    31981 aatatagcaa gttttttatt catgtttatc ttctcctttc ttattttcta tatcttcaag
    32041 gtccttatat atcgcctcaa aatttatact atcatcatta tattcatcag tttcttttat
    32101 gctttcttct tctctattgt agtaatcatt attatatgaa ttttcattaa tgtctttatt
    32161 caaatcttca tcatttaaag tagctttttt tcttttttcc atcttttcat ttgcctttat
    32221 ataaatattt tttctcttta tccaatgtat ataattattt agtaattgtt tacctagttt
    32281 tatggcaaat aataaaattt gccatgcaag tatctcaagc cctacaaatg ctatggttgc
    32341 gaaaatacaa aacatcataa catctggaac cattgctata aatttaatca caaatggaat
    32401 taatataaca aaagcaatta atgagcatac taatgcaata gtagcagata caatagcaca
    32461 tacaaacgca aatgctggaa caattagaaa taaagataac actactaata acccctttat
    32521 aacttgtctg gataatcctc ctacaccttc ttctatactt ggtgtaagtt tatcatttat
    32581 attttctttt gatttattgt acttttcctt agcttcatct ttcaagatag aataatcctt
    32641 ttttacattt gatttaaatc tactaaattt accatttaat atgtctgtat tcattatttt
    32701 tccatctata ttttcttttg atttactata cttttctttt gcttcatctt ttaagataga
    32761 gtaacctttt ttcatatctg atttaaattt actaaactta tcattcaaaa catctgtatt
    32821 tattattttt tcctcttcaa tatttttttc tttagtttgt aagataagtt cttttgctat
    32881 agattttggt gagccaagtg atgctattat ctccatatca ctctttccct ctataagacc
    32941 atctacgaaa tactcttcat aatctctcaa tatatcatta acttcgctct cagaaaaatc
    33001 tttttttaga taatccttta aaatttcaag aaactcagtt ttattcaagt tcattacctc
    33061 cctcatcttc acttattaaa atctcaacag cctttacaaa ttctctccac tcttccatca
    33121 gattggataa attctctttt cctagtgaag ttattctata gtattttcta gcaggacctt
    33181 cattagattc tagtatatat gtttcgaaat acaaatcttt tgtaagtctt cttagaatag
    33241 gatatattgt tccttcattt acatctatta ctttagagat gttatgtact atctcatatc
    33301 catacatatc tttcttagat atgagagcaa gtacacaaat ttctaaaaca ccctttctaa
    33361 attgtgtgtt catatactcc atcctttctt taaatacaca gtattgtgtt ttacaaggta
    33421 ctatgttaaa acttcttata tcctcataat aacacagtac tatgttctgc acaatagtta
    33481 tttttaaata ttcttgttga ttaaatcctt ctataaatgt ataatactct aatcagatat
    33541 attttatgaa agtggtgata aattggctaa aatgaaagat gataaaacaa tattaatcct
    33601 aactgctcaa tttggtgctg gacatataag tgctgcaaaa gcagtcaaag aatgtattat
    33661 tgaaaaatat agtaactata atgttgttat acaaaacttt ataaatgcaa gtattcccat
    33721 gatgaataag cctatggtaa aactttatga aaacaataca aaatatactc ctggattata
    33781 taattactat tattacttta agaaatcgtt tgattcaaga catgatttat ctcataaatt
    33841 atatacacct aaactttctg agtatattgc tgatataaat ccagatttga ttatctctac
    33901 atttccacta gctgctgctt gtgtaaataa ctttaagata aaaaatcctg atataaatat
    33961 acctacactt acagttataa cagatgttgt agatagtatg gaatgggttt ttgaaaatac
    34021 agatttgtat tttgttcctt ctcctgaaat aaaaaataga ttttttcaga aaggaataaa
    34081 tccagactcc ataaaggtta ctggagttcc tgttgataag agatttcaaa ttgaaagtaa
    34141 agaactatgt tgtgataaat atagactatt acttctaggt ggaggaagag gtctatttga
    34201 tatagatgag gattttatgc attggataga tgaatttatt gaagaacata gtaattctat
    34261 agaaattaca atagttactg gtaaaaacaa aaaattatat gacaatctaa ctcataaaaa
    34321 acctctaaaa aatattaaag tacttggatt tgtaaacgac atgtataatc taataagaga
    34381 atgtgaccta atgctaacta agcctggagg agcaactata tttgaagcta ttcaatcaca
    34441 aactccagta ctcgtaaaaa tgccaaaagt tggacaagaa attgaaaacg ctaaatttat
    34501 aatagataag ggtcttggca tgatttatag tgatgaccta gaccttaaaa acatatttta
    34561 taaattagta tcaaatgaat ttgattctat aataaacttt atgaaaaaaa atttaaaaga
    34621 atttaaaaca gtcattcatc cagagaaaat tgctgattat atatcagaat taatagataa
    34681 tcattacagt taaattgtac tattaaaaaa tttcaaacat aaaattaaag gcaatcaagt
    34741 atgcttttac acttgagaat ttactcatag tgtttaataa tacttgattg cctttatcat
    34801 atcaaaatca aattgttata ttagtatata tttttcttgt actgcatata cagttgttgt
    34861 atttcttcca tacttctctt taattctatt tgtaaatctg aagcagtctg gtaatcctta
    34921 ttttctatag cttctctcac tctagtgaat agtgattctc tagttaaagt atccttcata
    34981 atattgaact ttaaatccga tattgagttc caaactacct catcaagcgc atccatattt
    35041 tcttttgtgt gtatcttgac aatacttctt attttttgaa tcccagcctc tattattcta
    35101 gtttttaatt tcttttgcca cttatctata gctccaatct taaatgattc tattatacta
    35161 tcagtaaata catcaccttg ttttaaagat tttaattttg aagcatatat ttctaagttt
    35221 ttcatatttt cataaactgt agctggtggt attccaaatc ttgcatttct ttcttccaaa
    35281 ctataatgtt caaatacatc attttcatct ctatatgctc tatctttttc taaatagaaa
    35341 ctttcttcac ctacattttt agataactct ttttcaagtt cttttgtact aagaccactc
    35401 ttagctgctg cttttatacc atccaacatt gtttgataac atcctgctat tactaaatac
    35461 gtgttagata gagggcttgg tgaccttaac tcaaatctta cagtctttgg atttttcata
    35521 tctcttatta atccaacaag cactgaccta tttcttgatg gaatctcata actatgacct
    35581 aaagatgtaa ctatgcatac aggagcttca aatccaggaa ctaatctatt aaatccatca
    35641 ttagaagctg ttacaattgg gtttaatact tcataattgt aaagaagtcc cataagagcc
    35701 ccatatccta gttcacttag ataatcttct tttaaatctt ttggcgcaaa tagatttttt
    35761 atacttccat cctttaactt cgcacttact cctacatgtg tatgtccacc acttcctgct
    35821 actccatgaa ttggttttgc tttaaatgta acttctagtc catgtgatgt aaatacatcc
    35881 tctataacat ctcttactaa taattcattg tctgctgctt gtaacggtgt agaaaacttc
    35941 caagaaacct ctaattgctc catagcatgg tttgttcttc catctatact aatagaactt
    36001 tgtattcctc cgacctcttt gtgagccatt tcagggttta cacctagctt ttgaagtata
    36061 attaaacttt tctctaaaca agttcttata ataccatgag ttcttttcca gtattgctct
    36121 tttaggcttt gtgatacata taatttctct aagtcagctt tatcctcagg agtattaacc
    36181 cagaactcaa gttctgtagc tgctgttaac attatttcct ctatatcatc tactgaatct
    36241 attcctatgt tatttattac atgtggatat tctctaaata tttcatacat tgacttttta
    36301 aagtacttat ctgctttttg aagtactcct ctagaacata cttttttatt atcatgtatt
    36361 aaaaatgcag gtatctttaa tgttccaaca ggtagaccta cttcttcgtc tatatattcc
    36421 atattgtaat ctacatacca atgacaactt ttatctggca ttaaatctac tttcgcatta
    36481 tttaatgtag ctatattata aagctcaaca cttgaaccat cagtctgtat agctgattcc
    36541 aaaaatttat ctatatcctc caaaaataat tctacaggaa tcttttcatc tgttgcattt
    36601 cctcctaaat caacccccat taaggaaaca aatcttatat ttttattgct ttttaggatt
    36661 tctcttaatt cttcattagt gtgtttatct ttctctatta cgtataatag actatccatt
    36721 tttccactct cccataattt ttatgtatat tttattttgt taaatacaag ataaatttca
    36781 ttttccttac atttatgaat ttttcttgca aattattcgt gttttattcg tgtttttatt
    36841 tatattatta tatatcaagt ataaaaagtt cgcaatattt tttattggaa aaaatattat
    36901 acatacaata gttta
//
LOCUS       DAELTA010000018        36722 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.018, whole genome shotgun sequence.
ACCESSION   DAELTA010000018 DAELTA010000000
VERSION     DAELTA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.018"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(66..2021)
                     /locus_tag="KPG13_000795"
     CDS             complement(66..2021)
                     /locus_tag="KPG13_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984539.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(2204..3679)
                     /gene="spoIVA"
                     /locus_tag="KPG13_000796"
     CDS             complement(2204..3679)
                     /gene="spoIVA"
                     /locus_tag="KPG13_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG0984540.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3851..5146)
                     /locus_tag="KPG13_000797"
     CDS             complement(3851..5146)
                     /locus_tag="KPG13_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0984541.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(5330..6739)
                     /locus_tag="KPG13_000798"
     CDS             complement(5330..6739)
                     /locus_tag="KPG13_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0984542.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(6791..7930)
                     /locus_tag="KPG13_000799"
     CDS             complement(6791..7930)
                     /locus_tag="KPG13_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0984543.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            8249..8899
                     /locus_tag="KPG13_000800"
     CDS             8249..8899
                     /locus_tag="KPG13_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0984544.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(9056..10075)
                     /locus_tag="KPG13_000801"
     CDS             complement(9056..10075)
                     /locus_tag="KPG13_000801"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0984545.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(10106..10720)
                     /gene="plsY"
                     /locus_tag="KPG13_000802"
     CDS             complement(10106..10720)
                     /gene="plsY"
                     /locus_tag="KPG13_000802"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG0984546.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(10755..12074)
                     /gene="der"
                     /locus_tag="KPG13_000803"
     CDS             complement(10755..12074)
                     /gene="der"
                     /locus_tag="KPG13_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG0984547.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(12108..13436)
                     /locus_tag="KPG13_000804"
     CDS             complement(12108..13436)
                     /locus_tag="KPG13_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG0984548.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(13674..13874)
                     /locus_tag="KPG13_000805"
     CDS             complement(13674..13874)
                     /locus_tag="KPG13_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984549.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(13876..14808)
                     /locus_tag="KPG13_000806"
     CDS             complement(13876..14808)
                     /locus_tag="KPG13_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0984550.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(14853..15785)
                     /locus_tag="KPG13_000807"
     CDS             complement(14853..15785)
                     /locus_tag="KPG13_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG0984551.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(15840..17129)
                     /locus_tag="KPG13_000808"
     CDS             complement(15840..17129)
                     /locus_tag="KPG13_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0984552.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(17158..17850)
                     /locus_tag="KPG13_000809"
     CDS             complement(17158..17850)
                     /locus_tag="KPG13_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984553.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(17865..18626)
                     /gene="pgeF"
                     /locus_tag="KPG13_000810"
     CDS             complement(17865..18626)
                     /gene="pgeF"
                     /locus_tag="KPG13_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG0984554.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(18623..19084)
                     /gene="nrdR"
                     /locus_tag="KPG13_000811"
     CDS             complement(18623..19084)
                     /gene="nrdR"
                     /locus_tag="KPG13_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG0984555.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(19106..19369)
                     /locus_tag="KPG13_000812"
     CDS             complement(19106..19369)
                     /locus_tag="KPG13_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG0984556.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(19473..20246)
                     /gene="sigG"
                     /locus_tag="KPG13_000813"
     CDS             complement(19473..20246)
                     /gene="sigG"
                     /locus_tag="KPG13_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG0984557.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(20338..21060)
                     /gene="sigE"
                     /locus_tag="KPG13_000814"
     CDS             complement(20338..21060)
                     /gene="sigE"
                     /locus_tag="KPG13_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG0984558.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(21085..21957)
                     /locus_tag="KPG13_000815"
     CDS             complement(21085..21957)
                     /locus_tag="KPG13_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG0984559.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(22247..23521)
                     /locus_tag="KPG13_000816"
     CDS             complement(22247..23521)
                     /locus_tag="KPG13_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG0984560.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(23805..24962)
                     /gene="ftsZ"
                     /locus_tag="KPG13_000817"
     CDS             complement(23805..24962)
                     /gene="ftsZ"
                     /locus_tag="KPG13_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG0984561.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(25152..25505)
                     /locus_tag="KPG13_000818"
     CDS             complement(25152..25505)
                     /locus_tag="KPG13_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG0984562.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(25604..26278)
                     /locus_tag="KPG13_000819"
     CDS             complement(25604..26278)
                     /locus_tag="KPG13_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0984563.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(26283..26981)
                     /locus_tag="KPG13_000820"
     CDS             complement(26283..26981)
                     /locus_tag="KPG13_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG0984564.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(27004..27744)
                     /locus_tag="KPG13_000821"
     CDS             complement(27004..27744)
                     /locus_tag="KPG13_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG0984565.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(28014..29240)
                     /gene="murG"
                     /locus_tag="KPG13_000822"
     CDS             complement(28014..29240)
                     /gene="murG"
                     /locus_tag="KPG13_000822"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0984566.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(29258..30388)
                     /gene="spoVE"
                     /locus_tag="KPG13_000823"
     CDS             complement(29258..30388)
                     /gene="spoVE"
                     /locus_tag="KPG13_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG0984567.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(30440..31795)
                     /locus_tag="KPG13_000824"
     CDS             complement(30440..31795)
                     /locus_tag="KPG13_000824"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG0984568.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(31814..32782)
                     /locus_tag="KPG13_000825"
     CDS             complement(31814..32782)
                     /locus_tag="KPG13_000825"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG0984569.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(32823..34196)
                     /locus_tag="KPG13_000826"
     CDS             complement(32823..34196)
                     /locus_tag="KPG13_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG0984570.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(34363..36342)
                     /locus_tag="KPG13_000827"
     CDS             complement(34363..36342)
                     /locus_tag="KPG13_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG0984571.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(36354..36701)
                     /locus_tag="KPG13_000828"
     CDS             complement(36354..36701)
                     /locus_tag="KPG13_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984572.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
BASE COUNT        11286 a         6849 c         3872 g        14715 t
ORIGIN      
        1 tttcataata atgaaaggta ttacttagca atcgcatgat tttctaaata atacctttct
       61 aaaaattatt tttttaattt tatgaattta attatacttt ttctagtatc aggatttaca
      121 attaatatag aaccaatcat aattggtgtt agtatacaaa ataaaaaaat aatatctttt
      181 tttagattat attctttaga ttctaattta taattattat ttattattcc atcataatca
      241 cttatatttg acttattttt atttgtatct tgatttttgt tagaaatact ttccctataa
      301 ggaaaagaat gttcattggt accaaatact aattttgagc ttaaatcata attatcatca
      361 ttttctgtaa ttgaattttt attgtaaaat cctttttctt tatcagaatt attttcttta
      421 ttaccgtctt taggtatatc atgaggattt ttattttcat tgctatcagt acttgattct
      481 acatcatctt taggagtatt tggttgtttc tcatcagaat tatcattttc attattatta
      541 ttttcattat tattgttttg attgttatta ctatcattat tttcatttcc attattccca
      601 tcttgattgt tatcatcctc atctctatcc gggtcgtctc tacctcctcc tccacggtca
      661 ccatcaatat catcattttc ccgttcatca tctggtgtct cttggttgtc tccgcctccc
      721 attataaatc ctggtttaaa aactatattt gaataccctt cttttaatcc tcctaccact
      781 tttactctaa aacaacgcaa cttaaaatct tcaaaaaaaa gtatagcttg atctctaata
      841 tcctcctgtt cttcagatat ccaattgatt ccatcgcttg aatattcaac aaaaacctta
      901 cttgcactat acggcatatc ataaaataaa acagctacat atccattttt tgtattttga
      961 aattccagtt ccaaattgtc tattggtttt ttctctataa cagatacatt caaaatagga
     1021 actacatcat catttattat aattttttct ttatttaaca tttctgtttt aaactttcct
     1081 gttatattaa aattttctcc cttttccaac ttttcataat aatcagtaaa atcccattct
     1141 acatctatat caagtcgatt accactagat gtttctaata ccatatattt gggaaatttt
     1201 atattttcta ttttatcatc tttacttact tttatttcat cactaaatac accaattccc
     1261 ctatgacaac atttcacaat atcatagcca gaatcttcag ctatagaggg tataagctca
     1321 ttataatcat caattatata aactttctta ctttctgata taattgattg tgccaactta
     1381 ttattatctt ttagtaaccc actttcattg ttgctactat atgcttttat tgaagtttct
     1441 tctatctcaa catcaccttt agaataaaca cctatagcat gttctccatt cacttcaata
     1501 tccttattta gtattctaat atcattttca gtataaattc ctattgcatc tttcccattt
     1561 gcctttattt tttcaacaga ttcgtaggat gatttaatat gtaagtctcc tccttcttca
     1621 acgtataagg ctgtcccatc agtagcaatt atgttgttta taccaatact tatacttcca
     1681 ccattttcta tatgtattac tccttctttt cctccttcac ctattatatt caattcatta
     1741 tcatcaataa taaatctccc atgatttttt atcaatattt tatatttgtt ggtatctatt
     1801 gtaaaattac tgtcataagg aatataaaat ctaaactctt catctattac catatcacaa
     1861 gaaagagtat aaactatttc ttctttatta tttttatttt cttgaaccca cctttcaaaa
     1921 tcttcaagac aattttcctc ttgtccaaaa actttatgtg gaaaacttat aaaacacaaa
     1981 actattatac tcataaaaat aaatatctca tactttttca tcttacactc ctagaaattt
     2041 tttagctttg aaacaatctg tcgaaacata cacttcaccc actataattt ttccacaatt
     2101 atcaattatt gtcaatttat aaaagtatct ttattaattg ttataaataa aaagctactt
     2161 actaaagtaa acagacgttt cactttaata agtagctata tttttataac aaaatagtta
     2221 taatattaga acttccttca tttacaattt tttgtaaagt cttttgcatt ttaactctta
     2281 tctcttctgg catagtatat aatttacttt gtaattgctc ttttactaag tcatgtagtg
     2341 atttaccaaa catatttgat tcccataaat ctgctggttg ctcttcaaat acttccataa
     2401 gatattttat catctcttca ccttgatttt gatttccaac tattggagaa acctctgtag
     2461 atatatctgc ttttataata tgaagtgatg gtgcattggc ttttaacttg ataccatatt
     2521 gttttccttg tttcattatt tctggttctt ccagactaag ttcttctaag gaaggagcta
     2581 ctactccata tccttttatc ttggcatcta ttaaagcact ttctatttta tcatactcat
     2641 ttttaacttt agatagtcta gttatcagac taagcagttg ataatctcct tcaattttaa
     2701 atccactttt ttcttcaagg acattataga ataaatcttg ctttgtctgt aaatcaatat
     2761 ttataacacc ttctcctagc tctacattat caacacctgt atcttccaaa aactctaact
     2821 cagaaaatcc ttgtataata ccttctatat cccttatttt acctatatct ataatacttt
     2881 gttttaatgt tgttattatg ctacttttta tccaatgatt tctttcaaga ccttcaaccc
     2941 attttggtaa gttgattctt atttctgtaa gtggaaaatc atataataca gcttccataa
     3001 cctcctctat atcttcttct tccatttcaa ccacattcat tggaagaact ggaacttcat
     3061 acttttcttc caattcactt ctaagcatac tcgtttcctc actttttgga cttaacgtat
     3121 ttaaaaccac tgcaaatggt tttttaagat ttttcaattc ttgtataact ctctcttctg
     3181 gttctacata gctttttctg tcaattccag taactgaacc atctgtcaag acaactatcc
     3241 ctattgtaga atggtctttt ataacttttt tagttcctat ctctgctgcc ttttcaaatg
     3301 tcatagcttc ttgtgaccaa ggagtggata ctaatctttg ttttccacct tcctcatgcc
     3361 ctaaagctcc ttcaactatg tatccaacac aatctaccat tcttactttt aaagatactg
     3421 tgtcttttat ttttatttct acaccatctg ctggtacaaa ttttggttct actgtcatta
     3481 tcgttttacc tgaaccactt tggggtatct catctcttgt tctatccttt ttaaattcat
     3541 tgtctatatt aggtataacc aacttttcca taaattttct tataaaagtt gattttcctg
     3601 ttcttacagg tccaactact ccaatatata tatccccctg agtccttttg gatatatctt
     3661 cgtatatgtt attattcata ttattcctcc tatacatcta atcttattaa attatattta
     3721 gagttaaggg ttaatattct taaaatagaa aaataaagac cagcaatatt aatttaattt
     3781 atatatagaa atttgtaaat ttcaatgtaa attaaattaa tattgctggt ccaaatagta
     3841 gtgtattata ttaactgact ttagtagtat tttcatttga attattattc atctcaataa
     3901 gttctttacg tctttctttt gtcatgttca tccatataaa tgtaaacacc aatccaatta
     3961 tggctactat agtaagaact atgaagtagt acctatatcc agttacaccc ggaaatgcat
     4021 ccaatatctt ccctgctgct aaaggacaaa gtacttctgg aagatatcct aacgtagcaa
     4081 ctattcctat agcagttcct gatattgata atgaataatt accttcttca agtaacgaat
     4141 agtgtaaacc ttgaattgca tacatagata catatattgc aacacatcca atcaatagta
     4201 tataaatcat agatgatttt gttggtgtaa atattacacc taaaagacct gcaatcataa
     4261 gtataaatcc ataagatata atctttgatg aaccaacttt atctccaagt aatccagaag
     4321 cagctgatgc tactggtcta caatactgag ctaatattga gattgcagat gcaaacacca
     4381 cagtagctcc aaatgcttca gttgaataag gcgtaaaata ataaaatgac atattagctg
     4441 aatatgaaca aaacattatt attgttatta tccatgtatg aggcattttt attactgcaa
     4501 aaaaggcttc tttatcaaat tttgctgata cctctttttc attatccttt aatttgaaaa
     4561 atactaatac tccaactatt acatttattg ctgagtataa tactataata gctgtaagac
     4621 cagattcatc acttactttt gatgaaatat acccaaatag tatcaatact agagatagat
     4681 gaattgcatt ggtaattcca cgaccacttt ccataaatcc aaaagctttt ccttgttcat
     4741 tttcagatgc taacattcta acagctttta caagagctgg ccaaaaagta agtattgttg
     4801 ttattcccca taaggcatgg attaaaaaca caacaatata tggaggataa agtgttaata
     4861 caagaccaga tattcctgta ataattaaag aacctgaaag caattttctg gctgaaatac
     4921 ggtctgctaa aaaaccacca aaaaggtatg ataccattgc aagtagacca taaatgcttc
     4981 ctaagcttcc catttgtgta tttgtaagat taaatgcttg tgcaaaagta tcataataat
     5041 aattctttaa atatggtaaa gcatatataa atgctcctgc tacaaagagt attatcaaga
     5101 ctttgaaatt tttccaaatg cttggtttat ttacttcaac tgacatactt cctccttatg
     5161 taaatacctg taaattttca taataattaa ttattgctta ttttattgtt tattcaatat
     5221 ttaggaatat ttattaatat attaggttaa tctatcaata atttatggtg agttatgagc
     5281 taaaagtaaa ctcttttagc tcaccataat aattatattt ttatttatac taacatccag
     5341 agttattaga tatctttttt tcatagttct tccatagcat taaacctgtt attataacaa
     5401 aaagacctcc tcctatcatt tcaatctcat agattatatt atcttttaaa gtctccataa
     5461 ctggtgccgt tgagaaaagt atgccaacta cagttactac aagtaccatt ctggcaatat
     5521 tgatacaaac tgaatcactt tttgcaattg aatatggtct ttcttcattt ggtcttgtct
     5581 ttctaagtct aatataagat gtaaaaagca agacatatgg aaataatgct gttaatgctg
     5641 tcatagtaac aagaacattg tatatattat ctactgatgg taataagttt gttcctacaa
     5701 gaagtagact cactattata gcctgaacta ttactgcatt tgatggtata ttgtatttat
     5761 ttgtaacagt caattgctta ggaaatacac cttctcttac atttccaaaa agcatcttta
     5821 ctggacctgc tatataaagt attaatgctc ctagtactga taaagttatt ccaaatgcta
     5881 ctattcttat aaaccaactt cctataccta aatcttgtgc aactttagct aatgcatcta
     5941 gtatccctgt agatgctgca attttatctg gaggaagtat cattgttatt gctactgaac
     6001 ctaagatata gattcctcct attaaaacag ccacagttaa tatagcttta ggaaagtttt
     6061 tcttagcatt atccatttcg gttatgaaat tagcagtcgt ttcagctcct gataatgcaa
     6121 atattatagc agatatagaa actaatgaat tggcatctat ttttggtatt atattttgga
     6181 ttgtatatat cgatgctgat ggagcttttt tgagaataac tacagacata aatgccatca
     6241 ctataagtaa tatagcagga attgtagaac ctaatgctcc tgtattcgta aaaaattttc
     6301 caaatgccat cccacgagta cttactaagg acaacagcca aaatattatg agtgaaagtg
     6361 ctaaaataaa catcttattg tttgataaat ctggatttcc aagcatatat gcaatattta
     6421 tcgctaaaaa agtcaagaaa gaagaatacc aaaatatctt tgatgtccaa tttagccatg
     6481 acaccataaa tccccacttc tctccataag cttgtttaac ccattctcca agtccaccat
     6541 cttttggata tgttgcagcc aactctgcac atataagtga tgcaggaaca aagaatataa
     6601 aagcaaacaa taaccaaact ggtatagaac caagacccaa tccaaaacta gaggcagtag
     6661 atttagctat ccatctaatc ccaaatagtg ctgatatatt cataaatact aaatcccaaa
     6721 atccaatttt ctttttcact gtaattcctc caatttcaaa tttattcaga ctcatacaca
     6781 atgtattgac ttaaatatca agtataagtt cttcaacact tcttggataa ttagttataa
     6841 ttgtagcacc attcttacct actattgctg tatctgagtg tctaaatcct ccaactccag
     6901 gtatatatat ccctggctcc atagaaaata ccattccttc ttctaatatt aattcattgt
     6961 caaatcttaa atatggctct tcatgttctg ataaacctaa accatgacca attctatgat
     7021 ttgaatataa ctctagacca tactttgcaa caacagctct tgctgcttca tccaccattc
     7081 ttgctggtat accagctttt attgtatcta atccagcttg ttgagcctct actgcaatct
     7141 taaacacttc tttttgtctt tctgttggtt tgcctataat aaaagttctt tcattttctg
     7201 ctctatagtt ttcataccaa acctgtctac tatgtattac tatatctcct ctttgaagta
     7261 ttctagtatt ggaatctaaa tgaggttgtg ctgttctatc aatccctgaa caagtccagt
     7321 ttgcaaatcc tatatatgta tcaggataat tttcagaaac atattttagt aaagcattat
     7381 cccctgcaac gtcaaactcc aattcactca tcccaactct aacattttct aaagaccctt
     7441 ttattcctat atcagatagg taacctgcta tcttcaaaaa ttctatttct tttgcatctt
     7501 ttacatatct catttcaaat attttacttc caacatcttt gatagcaaat gacttatcag
     7561 ttatatactc tgaaaagctt gcagatacta tatctttttc tattccaaca gttgttcctt
     7621 tagggtattt atttaaaatt atatctaagt aatgtttatg tgatattcca tgttctttca
     7681 tttctggtat ttcataatac acatatacat tatcaacttt tgcaacttct ttagcatgta
     7741 attcttctaa tccaggtact attaattcta ttttatcttg agttactact gttacaattg
     7801 gtctagaata agttattgct ttaaatccac taatataaaa ttgattgtct ggttcaaata
     7861 ttaaagcaat ttcaatgctt ttttcattca tgtactttct caatctttct agtctcatat
     7921 tatttgacat aatgcacctc tttcttaata ttcagataat ttattcaata caacaaggat
     7981 gaatatatta ttttaaaatt cttaatattt ttagtacttg tcgtatttgt atgtaaatta
     8041 attttatttg tgattatttt atcataatca tgattatttt gtcaatcatg attttatttt
     8101 aaaaaatttt ttatattcta gtgattttaa attatctaaa atataacgag tatacattgt
     8161 attttgtaat attattcttg atttatggta agtttaatga taaaatcacg attatataaa
     8221 ctatattgat tatttgtagg tgaataaaat gacaaataaa gaattacaaa agaaaaggat
     8281 attgatgtat ttcatagaag ctgctcaaaa tattatggaa aacgaaggta tagaaaatat
     8341 tacacttcgt aaggttgcag atatggctgg ctataatagt tcaactctat ataaatactt
     8401 taaaaattta gaccatttaa tatcatttgc atctataaaa tattttaaag attataactt
     8461 gaatatttca agatgtatag aaaatgtcaa tgatgaatat aagaaatata taattatgtg
     8521 gaaattattt tgtaagcatt cttttgataa tgctcaagct ttttaccagg tattttttaa
     8581 cttgtctagt gatgaactta gctatataac aaaacaatat tatgatatgt ttccagaaga
     8641 tttaggtatt catgatagtg acatatcttt aatgattaca ggagaaagta taaaggaaag
     8701 aaataagata ctattaaaca atcttgtaga tactggatat ataccagcta aagatttaga
     8761 cataatcaat gatataatta tttcttttta tcaaaatcta ttattcttaa aaaagaacac
     8821 aggaaaaaat tctgatgatg atgaattaac tatgaaaatg atttcttcta ttgaatacat
     8881 aataaaacat gctaaataaa attaaaatat ggctgtctca aaatagagat aagccttttt
     8941 tattgttgaa ataataaaaa tatatttaat ttcatataaa aaagagtgcc tcgtaaacta
     9001 aaaagttcat tttgagacac ccctaaaata aatttattat ttattataca atagtttata
     9061 tatcatcacc taaaactact tcttccattt catgcttttt acttctcatc attagttcta
     9121 ttccaacttc tttaggattt gaacctttct ttattacaga atatatagca ttagttatag
     9181 gcatatctat atctaatttt tcagcaacat catgtgctac ttcagtagca gttatgcctt
     9241 ctacaaccat ctttacttct tttaaagtat cttctaaact catcccttta cctatgagta
     9301 taccagctct tctatttcta ctatgcatac ttgtacaagt aactattaaa tctccaatac
     9361 ctgaaagacc tgcaaaggta gacatattag ctcccatagc aattccaagt ctgctcattt
     9421 cactaatacc tcttgtcata agggcagctt ttgcattatc accataccct aatccgtcac
     9481 atatcccagc tccaaatgct ataatatttt ttaatgctcc tccaagttct actccaacta
     9541 tgtctggatt cgtataaact ctaagtttag ggctcataaa taaatcttgt atcatctgtg
     9601 ctatttttaa atctttagaa gcaactacta cggtagtagg aatatccctt gctacttctt
     9661 cagcatgaga tggcccagat agtattacat atggattttg tggtaattct tcttcacaga
     9721 cttgagataa tctaagccca gtcccttttt caagaccttt agctacatta acaataactt
     9781 gtccttcttt tataaatggt tttatttttt ggcaagtact tcttacagcc tgtgatggaa
     9841 ctgctaaaac tattacctta caatctttta tagttttttc tatatcagta cttatagtta
     9901 tattatttgg aaataatact cctggtaaat agtctgtatt ttctttagtt ctgtttattt
     9961 ttttagcttg ttcctctttt cgagtccaca tgctcacatc attacctttt tttgcaagcc
    10021 ctaaagctaa tgcacttccc cagcttccag tacctaatac acatactttc tccatgttta
    10081 acaccacctg agtataatat atttactatt ctttcttttg accaattttt ctctctgtcc
    10141 catttaataa tcgctttata tttgctctgt gattatatat tactgatgca gttaagaaca
    10201 gagctactat taaaccagct ttatttttta ccataatcat aaatattggt gaacatccta
    10261 ttcctacaac agaacccaat gaaacatact ttgttatagc taccaccaat ataaatactg
    10321 ctagacacat tagtgttatt actggattta cagctagcat tgcccctagt gaagttgcaa
    10381 cacctttgcc tcctctaaat cctaaaaccg ctggccaatt atgacctgct acaacacata
    10441 taactgccag atatccagct aaaagagtat ctattccaac caatcttgct gcaaattctg
    10501 atattaaaac agccattaca cctttaagaa catcacctaa aaatgtcatt gctcctgctc
    10561 tttttccaag tgtcctaagt acatttgtcg aacctgcatt accagaacct tgagtcctta
    10621 tatcaacccc agcaattcgc tttgcaacta tgtatgaggt agatatattt cctaaaagat
    10681 atgcaactac agcaataatt atataactaa atatctccac tgaatttccc ccctttagtt
    10741 atattttaag atatttattt tttatttttt tgtctatact caaactttat ggaagttcct
    10801 tcaaatccaa aattttctct tatcttattc tcaaggtatc tttgatatga gaagtgagtt
    10861 aaatccttat catttataaa caatgtaatt ttaggaggtc taatgtcagt ttgagtccca
    10921 tagtaaattt ttaatcttct acctttgtca gatggtggtt gatttaacat tacagcttct
    10981 ccaattactt cattcagtgc tgaagttgat attctcttag aatgttcatt tgacacatat
    11041 tcaactgtat ctagtatctt gttcattctt tgattagttt ttgcagaaac aaatacgatt
    11101 ggagcataca tcataaatgg aaatttttct tttatatctt ttgtgtaatt gctcatagtc
    11161 ttattatctt tctctattaa atcccattta ttgattacaa atatgcatcc ttttccttca
    11221 tcatgtgcta tacctgcaac ctttgtatct tgttctgtta caccttctaa ggcatctatt
    11281 actatcaata ctacatctgc tctatctaca gcactcatag cccttattac actgtatttt
    11341 tctattgtct catatatttt actttttctt ctaagccctg ctgtatctat tagaagaaat
    11401 ttctgtccat ttttttcaaa ataagtatca attgcgtctc tagtagttcc tgcaattgga
    11461 cttacaatta ctctttcttc tccaagtatc ttattgagta tagaactttt tcctgcattt
    11521 ggtttacctg ttatagctac tcttattata tcttcttcat actctgtatc taatcctgct
    11581 ggaaaatttt caactatctc atctaataaa tctccaaatc ccatactatt ggctcctgaa
    11641 actgcaaaag gcgttccaaa tcccaactca taaaaatcgt atatattatc aaattgactt
    11701 tgactatcaa tcttattaac cactaagatt actggttttt ttgtctttct aagcaattgt
    11761 gcaatttctt tatcagcagc tgttattcca gctttaccat caactataaa aagtattaca
    11821 tgtgacatgt ccattgcaag catggcttga tttctcattt gagataatat tatatcttcg
    11881 ctatcaggtt caattcctcc tgtatccact aatgtaaaat atttatctaa ccattctact
    11941 tcagcaaata ttctatctct tgtaactcca ggtgtatttt caactattga tatccttttt
    12001 cctgcaaatt tattaaatat tgtagactta ccaacgtttg gtctcccaac tacagctaca
    12061 actggcctac ttatactcat tttatttctc cttccatctt ttgttttcta ttttaaaatt
    12121 ttatccacaa aatcttttcc ttcattcaaa caagggataa tttttattct cataagttct
    12181 tctaattgat ttaattcaat attatctaag aaaatttctt catctgcttt tagcatactt
    12241 cttgttatat aaagagcttc tcctaattct ttatcttcaa gttgttcttt taaatcctta
    12301 gctgttaaaa gacctgatac agttattgtc tctccaaaaa acttgttttt gatttcatat
    12361 acatttactt gaagattttt aaacttgtcc atcatagcat ctgccatact ctgtataaat
    12421 tcatatgcag aatgaccagt agctattgat actttcttct ctctttttag aatcttctca
    12481 ggcaatatat ttagatactc ttcaatctct gtttttaact tacgtatcat acctacacca
    12541 tcttcaaact gtaaaaaacc ttcatattct tcatatccag gcaattcact attagatagt
    12601 atataaaatt catctgataa aaatgcaaat cgagtaccta atttttcaag atatttctgt
    12661 tgtatttgat gtatttgctc tatagtttta cttgcgctct tatcattaaa tatatttaat
    12721 tctactagat tttctctatg ctttgtaatc ccaactggaa ctatcgcaac actgtttaca
    12781 tacggataaa gttgagctag ttcttttatt gttctatcca attcctttcc atcatttata
    12841 cctggacata aaacaatttg acagttcatt tctatatgag catctgccaa tctcttcatt
    12901 ataccatata attttcctgc aaatttattg cttatcattt tttgtcttaa ctctggatta
    12961 gttgtatgca cagatatatt tataggactt attctatatt ttataatatt atttatatct
    13021 tcttcactca tatttgtaag tgtgacaaaa tttccttgta aaaaagataa tctcgaatca
    13081 tcatctttaa aataaagagt ttctctcata ccttctggca attggtctat aaaacaaaac
    13141 atacacttat ttctacagct tttagcttgg tcaattatcg gatttgtaaa ttcaacaccc
    13201 aaatcttcat catagtcttt ttctatctcg tatatgtaga cttcgccatc ttttttttgt
    13261 atttctacat ctaaatattc atcacttagt aagaatctat actctattat atcatgtatt
    13321 ggctgttcat tcacagatat aagtaaatct cctacctcta tacccatttc ttctgcaata
    13381 ctatctttat aaaccttact tataatatta tttacttttt ttacatttac ttccaatatt
    13441 ttcaccactc ttttgtattc atcaatgctt attataatgt attttaatat gcaatttatt
    13501 tatttattat attttatgat agcattttta agcatttcat gtcataataa cacattttaa
    13561 ttctaatagt caatataagt aaatagctat aaaatagatt agaacaaata atattcaaat
    13621 ttaagtttct taaaaattta aatattattt gttcttaaaa aaattttata aatttaatgt
    13681 tttactatga ttaacgtccc atttgtcata tcatgaacca ttccacctac acactttgac
    13741 atcaacaatg ccttattttg taagtctttc tcatcatttg cataaaatat tggacaacct
    13801 ccaccatttt tcatattctt atcaagtgta actatagcta gggtatattc atttaaacct
    13861 atatccattc ccatctcatg cacttccttt ctttcctatt ttataatttt ttagtgataa
    13921 gttttttcct ttagagcttg aaagtattgg gcatgacttt acagcttcca ttactttttc
    13981 cacattctta tctactggta aataagcaat cattatactt tcatcctctt tatttcttct
    14041 aggtaatggt acgaatgaag gttctccata atctccatat aaacctaacc ttgaatatat
    14101 attgtatact attgcttgtc tttgaccgga gtcaaataat attcctgcat tagcataatc
    14161 cttattttta ggaattattt gtattcctac acctctatta atatatactt ctctatcgct
    14221 ttttagacct atatttgtta taccttttaa atctcccacc tttagtatag attcgtccac
    14281 aaaactaatt tcagcaggca ttacatcagc tatgtctcca atactttttc tagtaagaag
    14341 tttttttaat ataaaggcta acactaatcc acaaattgaa cttattacca aactcatccc
    14401 ttcacttatt ttcgtttctg caagtattac ataatacaat cctattgtta aaaaagatgt
    14461 aactatacac atataatttc ttacttcata ggttctagct atctcttcta tgaatgcagc
    14521 tcctctcttt acaagttgaa cttcctctaa attttgcaat gtatttcttc tatttgctct
    14581 cacttgtcta aattgttctg ctgctagaga taaaaaagtt atagatgtat aagaacgatt
    14641 tataagagct ggaaccaata atgctcctaa tgaagaagcg acgaatgcta atataagttg
    14701 agatagtaaa acatttggtt gcgttggata ctgtttttgg tctaaattca acattataat
    14761 tctagatgtt ataccaataa taacagcaac aaaaaatact ctatccatta aatcactcct
    14821 aaaatattta atttgaaatt ttacatgtaa atctacaaag tctttctaga aaaatcactc
    14881 tgctttcctt ttgatgtttc tatgataggt gtacttttca ctacatctat catgacatct
    14941 atatctttga gtattggcat atatggtata accacagtac ttttttctaa attggtctta
    15001 gatatagcaa caatatcaac ctcatctaca tctctatcta tccccatatg aataaataga
    15061 ttatgtatta tagcttgtct ttgccctata tcatttacta ttccaaatgc acctaaatct
    15121 cttggtataa cttctatggc cagacattca tttctatact tttctcttgt actttctaga
    15181 cctatgttag ttattattac accatttacc attaaaatag gtccttcaaa acttattttt
    15241 gcaggaacta tatctgctat atctgctata ctgttccttc ttaaaaatct tctaaaaatc
    15301 aaaccaacaa tagctcctga cacaatagct aggattgtgc aataaaaaaa gctaaggcca
    15361 tactttcttg caaaaacgat ataaactatc gaagatacca gtgcagaaaa aagacttata
    15421 taacttctag attcatacgt agatgctatt tcttcaacgt aagcgtctcc ttttggtact
    15481 aattcttcat tatcgatatt ctttagtgtt attctttctt gttctgcaag accttgaaac
    15541 tgttgtattg ctactgcaaa aaatgtcaag gctgcaaact ccttatctat aagtgcaggt
    15601 aatgctatag cacctaacga agctgaaagc ccagatataa ttatctgttc taaataatct
    15661 tgtggtcttg atgggtattg tttgtctgta actcttaaaa ctaatcctct acataatatt
    15721 cctacaacta atgcaactat aaaagagtgt ctaaataaat ttgcattcaa taaactttct
    15781 tccattttct cagctccttc tcttatatag tttgtgcaaa aaacaaaaaa aattcccttc
    15841 tatttaagga aatttttttg taatattgta actataaatt cgctaccaat gccaagttca
    15901 ctttttactt ttatacatcc atttagtgat ttgactatat gctttgttat agccaatcct
    15961 aaacccgttc ctcctacatc cctacttctt gctttatcta ccctataaaa tcgttcaaat
    16021 atgcgttcta catcttcttt tggtatccct acaccattgt ctataacttt aattacgatt
    16081 ttttcttcat cttttaacac tacaacttgt acaatcccat tatcttgggt atactttata
    16141 gcattatcaa ctaggtttaa gaacacttgc tttatatagt ctttattgga tagtatactt
    16201 attgatttat catcacattt atataaaact tctatatttt tttcactagc tgaatgttga
    16261 gttatttcat atacctcttt aaatacatca tatactacaa cattttccat aagaagagta
    16321 tctttatttt ctacaaatga taacagcaat atatcatcaa tcaacctttt taatctatct
    16381 gattcgcttt ctattatttc taagaatcta tttctagtat tcacatctat attttcattt
    16441 gactttagtg tttcaacaaa cccacttata gatgtaagtg gtgttttaag ttcatgactt
    16501 acatttgcaa caaaatcact tctcatattt tcaagtttaa cttttttagt aatatcttct
    16561 atatttacta tcgaacctat tattacgttg ttagtattct gaagataaac tgggtctact
    16621 tttatcttat atacgatatc atctttagta gtaatattag cagtttcatt atctttagaa
    16681 ccaataaatt gaagtatttg ttttaatatt tcttcttctt ttattacaac attaatatta
    16741 tgaccttcaa ctaattgatt tggttcactc tttatcattt cttttgcttc atcatttatc
    16801 agaagtatat ttccatttac atctatagcc aaaattccat gagatatact ctttaatata
    16861 gaccttaact gtaaatgttt atactgtact tcttctaagg ttttatccat attctttatc
    16921 atatgattaa aatttctagc taattcgcca agttctcctt tggctgatat gttaagccta
    16981 gcatgatatt cattattact tacttttttt gagacgtata taaactcttt taaatatttc
    17041 cttaatgtta ttgtgtatct tacagctaat ataacaatgg ctatggacaa cataacaata
    17101 aaaaaatata tactatttaa atcttccaca agataacact cctaaaatat aaccttttta
    17161 atctattttg tatcctacac ctcttatagt ctctatatat ttttccgatt tatcttcatc
    17221 ttctattttt tttcttaaat atcttatatg aacatctact gttctagttt ctccatagta
    17281 ttcatacccc catattttat ccaaaagata atttcttgaa agtacttttc ctttattttg
    17341 aattaataat tttaacaatt caaattcctt aagagttaac tctatctttt cagagccttt
    17401 ggtaacttca tgttttgata aatctagttt taaattacct gtagtaagta tattgttttc
    17461 ttcacctaag ctagtcaaat tatatctcct taaaacagaa ctaatcctag ctaacaactc
    17521 ttttatacta aatggcttag ttatatagtc atcagcacct atttcaagcc cttcaacctt
    17581 gtcattttcc atatttttag cagtaagcat tataacagga atatttttta agtccttatc
    17641 acttcttatt tttctcaaaa cttctattcc acttatattt ggtaacatcc aatctaaaag
    17701 tattaaatct ggtttaattt ccttagcctt tataaatccg tcaaatccat catatgaata
    17761 actaacttcg taatttgaca cctccaaatt gaattttaat agctcaacta tatgcatttc
    17821 atcgtctata acaagcactt tagtattcat taaaaagctc ctctctattt tatctgtagt
    17881 accgttccta ttcgttctga tgccttatta tgttttctat aagagtggaa tttatctgct
    17941 ctacaactag tgcataaatt taaatttatt atattttctt ttttaactcc actacatcta
    18001 agcatatact cattaatttt ccataaatct aaatagtaac ttccctcttt tattatatag
    18061 aacttttcat cattatttgt aagaattgtg ttaaattttt ctactaaatc ttcagacact
    18121 tcataacaac atggtccaat tgatggccca attatacata ctaaatcttc aggaactgtt
    18181 ttatagcatt cagacattaa ttttattgtt ttatgaccta tattactaaa agtccctctc
    18241 caacctgcat gagccagtcc tatggcttta tttttagcat ctataattga tataggtaca
    18301 cagtctgctg taaacagtaa aagtggtact ttttccaagt ttgttataag tgcatctcca
    18361 tctcttcttt tccctatagt atctttattg actatattaa ctatatctga atgtatttga
    18421 gaattactag ttagattttc aaatgtcaaa tcacattctt tacatacttt taacatatct
    18481 tctctatttt ttgcatctat attcttacat gttataacta gatttacaga atttaatttt
    18541 tcatcaagtt cttcacaatt accacttaaa ttaacatact ctttaatatc ttgtacattt
    18601 tttatattta tataatcttt catcatttgt ctcctttttc tatcagtata cttttaagtt
    18661 catttacaaa agtatttata tctttaaatt gtctatacac agaagcaaat cttacatagg
    18721 caacctcatc taactctttc aatttatcca ttaccatctc accgattttt ttggtttcaa
    18781 tttcttctat aaaacattta tttatttcat tttcaatatg tacaacaata ctttcaatct
    18841 cgtcaataga aacaggtctt ttttcacatg cttttagcaa tccatatttt atcttttctc
    18901 tatcaaaata ttctctacta ttatcctttt taatgaccat taaaggagtt gtttctattt
    18961 tctcatacgt agtaaacctc tttttacatg attcacactc acgtcttctt ctgatagatt
    19021 ttaaatctgt atgtctagaa tctattacct ttgattcttt ataattacaa taaggacatt
    19081 gcatctatat tcctcctttg tttaactata tctcaaactc atctaagctt aattctgaac
    19141 ctatatttac tattattgta tcacaaccta ttttaagtat atcattccaa aatataactt
    19201 cttcatcccc tcctcttgaa aaaaaacttc tacttccatc tccaaaaatg gtaaatgaga
    19261 ctaccttacc ttcgtgaata tccatgtcta tgtctattat aaaccccatc ctttttccat
    19321 tttttacatt tataatttct ttttccatta tatcagaaac tctaatcata ttgtactcac
    19381 cctttcttta aaaataaata tgtatcgaag taaaaaaaaa tgacctatat aggtcattta
    19441 aattaaaata atgtttacag tccatggatt acttatacat attttctcat attttttaaa
    19501 gcattttttt caattcttga tacttgtgct tgagatatac cgatttcatc agcaacttct
    19561 atctgagttc ttcctttgta aaaccttaaa tctaatacca acttttctct actatttaat
    19621 ttcttaatag cttctttaag tgatatttct tgcaaccaat tttcatcagt atctttttta
    19681 tcttgaacct gatccataac gaatattgca tctccattgt cttgatatac tgggtcaaac
    19741 agagatattg ggtcttgtat ggcatcaagt gccattacaa ctgattctac ttctaattct
    19801 aattcctttg ctatttctga tactgtaggt tctttagaat ttgttctaat taatctttct
    19861 ctaacttgta gtgccttata tgctatatct tttaatgacc tacttactct aattgggtta
    19921 ttgtctctta gataccttct aatttctcct ataatcatag gaacagcata agttgaaaac
    19981 cttacatttt ggcttaaatc aaagttatct atagctttta taagaccaat acaacctatt
    20041 tgaaataaat cgtctatatt ttctcctctg ttgttaaact tttttattac actcaagact
    20101 aatcttaaat tccctcttac aaactgttgt ctggcttctt catcaccatt ttttatttgt
    20161 aataaaagtt ccttcatttg cttattttta agaactggaa gttctgatgt attaacacca
    20221 catatttcaa ccttattaac ttgcatatat tttcagtcct ctctcaaaag tattcatcta
    20281 ttgaaattat ttacatttcc aattttttta tactttttaa gaagaattaa tttagcatta
    20341 tacaaatttt ttcatttctt tttgcaacct tgagattatt ttcttttcaa gtctagatat
    20401 gtaggactgt gatattccaa gcattccagc aacttctttt tgtgtttttt caataccttt
    20461 atcaataaga ccaaatctta attccataat ttgcttttct ctgtcactta atctgtcaag
    20521 tgccataacc aacaaatctc tatctatttc ttcttcaatt attttgtata tttcatcatt
    20581 ttctgtaccc aatacatcag ataataaaag ttcgtttcca tctaaatcta tatttaaagg
    20641 ttcatcaaat gatacttctg ttttcttttt attatttttt cttaagtaca ttaatatttc
    20701 attttctata catctcgaag cataagttgc caattttata tttttattca gtttaaaagt
    20761 attaactgct tttatcaaac ctatagttcc tattgaaatc aaatcttcta catctatacc
    20821 tgtattttca aattttctcg atatgtagac tactaatcta agatttcttt ctattaaaat
    20881 tgactttaca ctttcatctg tttctagctt ttgtaaaagc tccatttctt cttctggttt
    20941 caatggagga ggaagtatat ttgcccctcc catatagtat attccctttg gctttattat
    21001 ttttaattta atacctaaca tagtaataaa gcttattatt ctttctttca atcgtaacat
    21061 tccttgtccc ccttaaattt tcacctaaaa tccaggcaat atatttggat tcattattgc
    21121 tccatattct ctatcattaa aatttgaaat accaagtatt atatttccta tcttttgctc
    21181 atctacttct atatagtcag cttttaagcc tattattatg cttgtcttat tacttccagc
    21241 atgtttataa ggtattattc taactctacc tgaagtttta tcatctaaac ttcctataat
    21301 ttcttgtgct ttcttcatat ctatatcttc ataattaata tttaatagtt tttctggaat
    21361 aaatccttct aacacactag acttaactat tattacatca ctttgactta ttgggtcttt
    21421 taacaaatta ccactatcta ctaaagcctt acagcaaaaa cttttttcta gtaagtttat
    21481 atttatagtc tttgtaagct ccttaatgta cttaagcatt ttcaaatctt tataaatgta
    21541 ttttaataat tcacaactca cataagtaca tgcaattaag aaagatattt ttaagtgact
    21601 tattcctgta aaataaatta taaaataagt gcttccagaa ataaaaatgt ttactagata
    21661 aaaaactaac gctacatgaa cataatcttt tttgctctta cttctaaatg aaattaaagt
    21721 tattatactc atgattatta ttttaaatgg tacagtaaat agtatattca ttgatggata
    21781 caaataagca acagaatagg cacctcctaa actagccccc aataacttat tttttttagg
    21841 attatatctt ttagtaagaa tagaagtaca acttataata atataattta ttactaggtt
    21901 ttctataaca tagtactcaa tatacaccat gtatcccccc ttaaaacctt taaacatatt
    21961 ataagatagt atgtacaaaa ttttatgtca cttttgatgg tcgaatccaa aacaaattct
    22021 atttaaaaag aacaatgata tatgtcttat actttatgtc gtcaaaagat atttttttaa
    22081 taccaaacaa atagactttt ttgtagaatc caatatatct aggtagaagt tattcactct
    22141 acttttggta tatttacaaa aaaagactac ataatttaat aatatatcaa gtaaatcttt
    22201 tacttatata aaatcaaatt atatagtctg taaattcaaa tataaattat tgtgctagta
    22261 atccatttgc atattttgca gattcattta gtgcttgttc tggagttatg tctccagcta
    22321 tcatcatttg aagttgtttc cataaatatt catatatctc tactccatga ggtccaactg
    22381 tcaatggtct atacatatgt ttatctcttg tcaccatttc ttcaaatctt gggtctcctt
    22441 ggtatttttc acctttagca actggagctc taggtatttc tttgtggaaa gcttccattg
    22501 attctttgct tagcatatac tcaagaagtt taaatccaag ttctttattt ttacatgctg
    22561 acattaatgt taaatcatct actgaagcaa aacttccaac tgtcttatct tctaatgatg
    22621 ttacatatcc ccagtttaaa tccgggaatg atttatcaaa agtacttgta gcagcagagg
    22681 atagcattat agtaaatgca gcttttcctg gtccaaatac actttcaatc atttcattac
    22741 tatcttttga taaagcatct tctggaatat actttttcat atcatttata aagtttgcag
    22801 ctttcaagcc ttctgcatta ttaaatttaa ctgattttaa atcatcatta tatatatctc
    22861 caccagcttg ccataaatac ggataccagt tccagtttaa gcttccaaat actttagaac
    22921 cccaaccttg tgcaagaccc cactggtcga tttttccatc tccatcagta tcttttgttg
    22981 ctttttcaca gattctttta aaatcatccc atgtttttgg aggtttttct cctaattttt
    23041 ctagtatatc tttattgtag tacaatacgc ctggattagc agcttctata ggtaaaccat
    23101 aaataccacc catcattttt gcatcatcta agtatagata attatctgaa gttcctgatt
    23161 tttctaaata aggtgttaaa tcttctaccg cacccatttc tataaattga ggaaacatct
    23221 ctgcatacat gtatcctata tctggacctt cacctgcact tattgctgtt gcatattttt
    23281 ctgcgtagtt atcccatggt actatctgaa attcaatttc acagttattt tctttttcaa
    23341 atttttcaaa tatcggcttc catgcatctg catcatggtc tgttaatggt gcagaccata
    23401 caactaattt ttctttaccc tctttgcctt tagaatcttc tttttttgaa catcctgcaa
    23461 gaattgatac tgatagaatc cctaccataa ctaaagaaag aacctttttt agtttaatca
    23521 tgcttaatcc cctttcagac tttaatttgt ctttttcctc aaatgtttac atttaattca
    23581 gatttaatat taacaaataa gattactctt gtctaatcat aaaaaactat actaactcct
    23641 ataactatat tttttttcta tacctattag atttaattca tactaaaaac ttacttttta
    23701 aattttatca atatttaaaa atttttttaa gtctagccac aaactaacta tctaaattga
    23761 gcaaaataaa aagaagtcta ttgacttctt tataagctta catattatct tcttcttctt
    23821 aggaatgtag gtatctccat gctctcatca tcatcttctt caattatact tgccttttta
    23881 ggaggctcta ttttttcctc agcaattact tcttcttcaa tttcttttac agcttgtttt
    23941 actgttgtat ttaatgaaga acgtatgctt gatttaggtt tagtatctaa atctaaggca
    24001 ccattttgta atccttcaaa tcctgtcgcg attactgtaa tcattatttc atcacctaaa
    24061 tcttctttta tagaagctcc aaatataaca tttgcttctg ggtcacagga ttccataact
    24121 aaagttgatg cctcattaac ttcaaataat cctaagttag gaccacctgt aacattaaga
    24181 agtactcctt tagctccttg tattgatgtt tctagaagtg gagattgaat agcttgtctt
    24241 gcagcatcaa ttgctctagt ttcaccacta gcactaccta tacccatatg agcaagtccc
    24301 ttgtctttca ttatagtagt aacatctgca aagtccaagt ttattaaccc ttcaactgct
    24361 ataaggtctg aaattgattg tataccttgt tttaatacat catctgcaac tgcaaaagca
    24421 tctaacattg atgtattttt ttgtactatc tgtaaaagtc tatcatttgg tatcgttata
    24481 agagtatcta cttttgattt aagttctgct attccacctt cagcgttctt cattcttatc
    24541 ttaccttcaa atgcaaaagg tttggtaact acacctactg taagtatccc catttctttt
    24601 gccaagcctg caaccactgg agcagctcct gtaccagtac caccgcccat tccggctgtt
    24661 acaaagacca tatcagctcc ttgaagtaac tttactattt catctttact ctcctcagca
    24721 gctcttttcc ctacttctgg atttgctcct gctccaagtc ctctagttaa tttttctcct
    24781 atttgaactt tatattcagc ttttgatgta tataaagctt gtttatctgt atttactgat
    24841 ataaattcaa cgccttttag ttgagcttct accattctat tgacagcatt gtttccaccg
    24901 ccacctacac ctattacttt aatttgtgca cattcttcta attctacgtc aaagtttagc
    24961 atcataaacc cccttctctc atgattcaaa gttatcgtat gtattaatta tatcattttc
    25021 ttcttttatt ttgttaacct ttaagtaaca tattccacaa aaacgtaaat tttccttttt
    25081 aaatttctta cttatattgt caaatattac tctttaagta taaaataatt tacgtatata
    25141 ttaaatgctc tttaattttt acgttttttg ttcataatat tactaataat atatcgtcta
    25201 attatagata agttattaaa caatctcata ccaaaaacta atacagcaac atagtaaaga
    25261 gaaactccta acttttctcc tatataagtc aataatatag ccaatatagc attacctatg
    25321 aaaccagtaa taaaaattac attatcatag ttatcttcaa aattggattt tatcgctcca
    25381 aaaatagaat ccattattgc aagaatagct actgacatat atatggaata atccatagaa
    25441 tatgtaaatg gaatatagta acctactact acaccaaatg ctaatcctcc taaaacccat
    25501 aacataaatc ttcacctctt tattttactt tgaaacacaa ctacattaca atccttttta
    25561 ttaatttttt ttactttttc tttatttttt tgtatttttc gttttatttt gcaaataaat
    25621 ccactttatt tccattattt tcctgaactt ttatattaat tccatatata tccttaagag
    25681 atttagcata tgattctgga gagtttatag ctgcactcaa taaatctata tctcctatag
    25741 cttttattat aaatggttgt ccatatgtag tatcatttac tgtaatagta gctcctgaac
    25801 attttatctt acttgttgct agtactcttt ctccatttat agaaattgct cttgctccag
    25861 ccttttttaa atcatttaaa accctcaaaa tatctatatc atgtataatc aaatcgtttg
    25921 gattctgtcc gtcttttaac tctctctcac tatccctcat agtaatagtt atgcctggcc
    25981 ctgtaacagt tgaataacca cttaattctt taacttcttc aagctctttt tgcataagtg
    26041 tttggataga ttcatttcca ttcttttttg cttctttata ttcattcaaa gtttttttgt
    26101 tgtttttaag agtttctttt aaatttttta tttctttttt tgtattttct acctcacttt
    26161 caatactttc agtttgacct aatgttatgt atactctatt tgggtctagt gattttatat
    26221 aagttgatat gaaaacgcct aatatgaatg ctcctaatat aactattttc tttttcatac
    26281 tattatttcc cctctgcttt gtttatatca ctatgaccat ttatttctat attgtcttct
    26341 ttggtcactt ttactttggc attataatat gtttctattt cccagacaat accatacttt
    26401 atattaagtg ccgaagccaa tgtatcagaa tcaccaattg cttttattat aattggttct
    26461 tttatgacag tgtcattcat ataaagcttg tcttctttta aatacatata tgtattagaa
    26521 actattctct ggtcgtttat agatatggca cttgcttgtg ctgaatttaa tttatttatc
    26581 attgacaata ataaatcata attatatagt atactatcat ttttatcaga gtttcctaag
    26641 tcttttgtag aatttatctc aatttttatg cctgttcctt caacatctgt atatccagct
    26701 aatgtttcat atttttctat ttctgactta agtatgtctt gttctccttc attgtttttg
    26761 cctctataag tctctatatc tttcttcaca ctattaactt ctgattgtag attctctttt
    26821 tcttttttta aagatttaag ttcttctgta agttgctctc ctcttttaaa agttgtcatc
    26881 cctctatttt ctatattaat aattttaagt tgtaaactaa caagaacacc caatacaata
    26941 cttcctatga ttatactttt atttataaat ctttttttca atatatcact tccttacaat
    27001 tgtctatcct tctggactat acactgccaa ctttccatag ttaagattta tagtccctcc
    27061 attagtattt ttattttgca ggtcaatcag aattttactt accctagata tattataatc
    27121 taagttatca tcattagaaa gtaagatttt tatattactt cttgtaagca tttctatatt
    27181 gctttctttt tttaagttta cataatttat ttttctataa attccttctt cttttaaata
    27241 atttaaagtt ttaaatacat tctccttagt tttataattt ttaaatttta tagatttatt
    27301 atcatcaatg ctataatcta catctactat caagtctttt ttactttcat tagaacctct
    27361 tagttcttcc aataaatttc cttttttatc tatataacaa taattatctt catcttttaa
    27421 tactgcaaat atttcttttt cttttaaact tataattatc ttattgggaa gttttctttt
    27481 tatttccaca ttttctatat atggattttt tataagttta tttttcatat ctttaaaatt
    27541 gtatgcaaaa atattttcat ttagatttac atttagttct ttcattatat ttgatttggt
    27601 caccctctta tttccaatta catctatttt ctttacatca aataaatcac tttttacaat
    27661 tatacatata caaaaagata acataagtat aaaaactaaa actatcatga cattattcgt
    27721 atttaacttt cttctttttt tcaaatataa tcaacccctt tggttaataa atacaccttt
    27781 agagcttgtt tttcctacaa aaaaattata atatttaaag tcaaattttt gcattttatt
    27841 acataacatc caaataaggt attaaatttt ataaataaat caatatttca tcaaaataaa
    27901 catagcatac tacatactat ttatatattt aaatctgtat acatacaaat aaacccctaa
    27961 tttataataa tttagaaaat ctaatatatt ataaattagg ggtaaatatt tacttatctt
    28021 ttctttattc ctattacctt agcttgacct tctatacttg gagtcgtttc tttttttatt
    28081 tcttttactt ccttaataac tttttctttt tttgtttttt tagaagttga cttttgtgtt
    28141 gaattgtata ctttcattat ctcatcatat ataaggtcta tggcttcagg cttacctata
    28201 gttttacttg catttgccat atcaaccaaa agttctctat ctcctaaaag tttaaatact
    28261 gctgtattta ggctctctgg agttagattt ttttctaaaa tagcaattcc tgccccttgt
    28321 ttttctatac tcttggcatt atactcttga tgattttcag cagtataagc tttaggtatt
    28381 attatagatg gctttccaag agccgttatc tcagctaatg aaattgctcc tgcgctacct
    28441 ataaccaaat cacttgctgc aagtgcattt gccatatctt ctaagtaagg cacaactttt
    28501 tggtatggtt taagatttat atcgctaata cttcccataa attcatcata atagcttttg
    28561 cctgtagcaa atataaaagc tatatcttca tttaccatat tctttattac aagtctcatt
    28621 gcatcattta tttttcttga acctccacta ccaccatagc aaagtaccat tcttttttcg
    28681 tcacttattg atagattctt tcttgcaata ttttttcttg ataataaaat ttcttttcta
    28741 actggatttc ctgtaaaaac taatttatct tctgctgcct ctggaaatct tttatgcgaa
    28801 tcttcaaaac ttgtaagtac ctttgtaact gttttagata aaatcttatt agttacacca
    28861 ggaaaagaat tttgctcatg tattatggct ggtattttcc ccatagatgc attaaataaa
    28921 acaggtccac taacatatcc accagttcct ataactatat ctggtttaaa tttcttaaca
    28981 atctttcttg attgctccaa acctttaaaa agcttaaaaa ctctttttac attatcgaaa
    29041 tctatttttc gcttaaaacc ttgaactgtt acagtcttta attcaaatcc atacttagga
    29101 actatttctg actctattcc cttttccgtc ccaacaaaaa ttatctcagc gtctggatgc
    29161 tcatctctta ttttgtttgc aatagctata gctggataca cgtgccctcc agttccaccg
    29221 cctgataata aaactttcat caaatcatca tctcctgtta attaattttg acatgctttg
    29281 agatgtttaa cactattcct acagctccca taaagatggt gagtgaagtt cctccataac
    29341 ttataaatgg tagtgctaca cctgtagctg gcattgatga tgtcgcaact gctatattta
    29401 aagctgcttg tattccaatt tgtgcaccta tacctataac aaccatacaa gcaaatacat
    29461 tagaagtctt caaagctatt cttacacatc tgtagactaa aactacaaat agcataataa
    29521 ctattataca accaatcaat cctaactctt ctcctattat agcaaatata aagtcatttt
    29581 gtggctctgg tatataaaaa tacttttgtt tactctttcc taagcctaga ccaaacaatc
    29641 cacctgaacc tagtgcatat agaccttgta ttacctgata ccccttacca agagggtctt
    29701 gaaaagggtc taaaaatgat gtaacacgac taagtctata gggttcagca attactaatg
    29761 cagcaaataa agcagcccca ctccctatca tagcaaatac aattttcata tccattccag
    29821 caacaaatat cattacaaaa gtaactagaa ttacagtacc tgctgttgac aaatttggct
    29881 gaagtattat caacgcaaaa aatatccctg gaactacgag aagtggtaca acaccttttg
    29941 tcaaagattt tattttatca taattttttt caattaattt tgcagttaat attattgtag
    30001 caaactttgc gatttctgca ggttgaaatg ttaaagctcc tattcccaac catctttttg
    30061 ctccgtttgc ttctatccct aaaggggtca aaactaacaa taataatact actgctatag
    30121 ctccaattgc tgtagcattt ttcttccaaa aactataatc tattcttgat gtaataatca
    30181 taactataaa tccaagtaca gcatatatta cattcttttt tagaaaatag tacgcatcat
    30241 tgtgtttaaa tgaagattgt ataaaacttg cactaaacac cataactatt cctacaaata
    30301 ctaaaagcat agtagtgtaa aacactactc catcaaattc agtcttcacc cttatgtcaa
    30361 tttgtttttt caaattttct ttaggcatcc aactaatcta cctccattct tccatttaag
    30421 taagtttaga ggtaagagat tattttaaat tgtttacatt atctttgaag tcttttcctc
    30481 ttacttcaaa actttcatac atatcccaac ttgcacaagc tggtgaaaga agcactatat
    30541 ctccactttt tgaaatttca taagaagtct taactgcttc ttccatatct tttactatat
    30601 gtatgtcatt aaatccttta tttttagcac aattttctat atttgatgca gtttctccta
    30661 ataagaccaa tgatttaaca tatgactttg cagtttcaag aagttcatca aaattacttc
    30721 ccttatccat cccccctgct ataagtatta taggtctatc ataagactgt atagctttta
    30781 tagtggaatc tggattagtt ccctttgaat catttacata tattacattg tctaaatttc
    30841 ttacaaactc ttgtctgtgt tccactcctt taaatgtcat aagtacatcc tttataactt
    30901 ctaaatcaat tttgcagaca tatgctattg ctatagctgc catacaattt tctaagttat
    30961 gacctccagg taaacttaca tcatctttat ttagaaattt gattttatca tctatatcta
    31021 ttattatgtc attattttct aaatatactc ctccatttac ttttttagtt cttgaaaagt
    31081 aaattacttt ggcattgcat ttatcagcta aagattttac tatctcatca tcatagttta
    31141 atacacagaa atcctcaaca gtttggttca taaatatatt tgcctttgct tctatgtatt
    31201 tttccatagt atggtgtcta tttaaatggt cctctgtaat atttaatatt gcgcttactt
    31261 taggtctaaa ttcatctata ctctcaagct gaaaactgct tagttcagtt actaacacag
    31321 actcttcact tgaagtttct atagtatcta ttacaggatt tcctatatta ccaactacat
    31381 atgtatctcg ttttgctctt gaaaaaattt ctcctaccaa actagttgta gttgtctttc
    31441 catttgtacc tgtaatccct ataaaaattg gattatccga tagtttaaaa gctaattcta
    31501 cctcaccaat tatatacttg cctgaatttt ttaatttcaa tatgaatggt aggtctagtg
    31561 gtacacctgg agacactaca accatatcta tatcatctac atcttctggg tgatgtccta
    31621 atatgtattt tatgtcattt atagatttca attcatcaag tatatttctc aatttatttt
    31681 catcttttat atcatttacg attatacttg cacccaattt atctaaatgt ttaattgtag
    31741 atataccagt ttttgcaagt cctactaata aaactttttt tccttttaag tccatttttc
    31801 attcccccta gccctaaaat actgctatta tacttatcca agctaaaaca actgttataa
    31861 tccaaaaaat aaatacaact cttgtttctg gccaaccaca ctgttcaaag tggtggtgta
    31921 ttggagccat cttaaagaat ctctttcttg ttgccttaaa atatccaact tgaatcaaaa
    31981 ctgatagagc ctccgcaaaa taaattcctc caattatagg tattataagc actgagttag
    32041 ttagaactga aaatgctaca actgcacctc ctaatgccat tgaacctgtg tcgcccataa
    32101 acactctagc cggatatgaa ttaaatccta agaaacctaa acatgctcca acagttgctg
    32161 ctgctaaaac tgcgacttct gtatttccag caatagaact tgcaaatagc ataaagaata
    32221 cagaaactat taatgttact cctgatgcta atccatctaa tccatcagtc aagtttacag
    32281 catttactat tgctacgatt ataaacatca ttattggtat atataatatt ccaacattta
    32341 tcacaaaatc tgtaaatggt atcataagtt gtgaagcact tgatgatgat gtatactgat
    32401 aaaaagcaac ataaaaagat aatgcaactt gaaggattat tttttgatat gcttttagtc
    32461 caagtgatct ttttagcttt atttttataa aatcatctaa aaagccaata aaaccaaaac
    32521 ctgcaataca tataagacct actgccatat catgactaac ttttactctt gtaagtccag
    32581 ttattagaat tgcaacaatc attataattc cacccatagt aggtgttccg tttttagcta
    32641 gatgtgtttg gggtccatca tctctaactg tctgaccaaa tttaaatttt ctaagcattg
    32701 gtataaatat tggtcctatt ataattacta ttaaaaacgc aatcaatgcg gtgtacgtaa
    32761 gctctgttat tcctaacatc ataaaactcc tctcccttgt caattaactc attagtttaa
    32821 gcttatttat ttagatattc gactattttt tctaatctca tgcctcttga gccttttact
    32881 aaaatagtgt ctcctgtttt tactaattca tttaacttgt taaacacatc ttctctattc
    32941 tcaaagtgat atatattggc tgaattgaat cctaattgtt ttgcttcttc tccaataaac
    33001 actgaatttt cacctatagt tattataata tcagtattat tcatagatga ttttccaact
    33061 aatctatgac catactctga aatttcaccc atttctagta tatctccaag tatcgctacc
    33121 cttctacctt tatatcttcc aagtatcttt aaagcagcat ccatagaatc tggactagca
    33181 ttatatgaat cattaattat tgttaatttt tcatttttta ttatgtctag tctcattttt
    33241 gtccctttaa aattttttaa accttttttt atataatcta aagaaatatt tagacacaaa
    33301 ccaactaata tagcagacat tgcattatat atattatgtt ctcctattgt tggtataaat
    33361 atttcctctt ttttcccatc tattacacat ataaatgtta aactatcctc ttccatagta
    33421 taactttcac aataaacatc attgttttta ttaaagccaa atgtttttaa cttatataca
    33481 tgttcttttt cttttaatgt agataaaaac ttatcatctc catttacaat taaagtattt
    33541 gtttcatcaa agtttgaagt aatctccatc tttgctttca aaataccctc ttgtgaacct
    33601 agtttttcta catgtgataa tcctatattc gatatgactg caattttagg atttactata
    33661 tccactaaat attttatttc attgaatcca gacataccca tctctattac agcacactca
    33721 tgttctttat ttagattaaa taaagtcaat ggaactccaa aatgattatt taagtttttt
    33781 tcatttttta gtatatttaa ctttgcagat atggcagagt aaatcatatc tctagtagta
    33841 gtttttccca cactacctgt aacacctata aatggaatat caaatttttc tttatagtat
    33901 tttgaaatat ctcctagagc caaagatgta tcttttacct ctattaaatt tatatccgaa
    33961 ctttctaatt ttattccatt actttcattt tttataaaag ttttacatcc actatcatat
    34021 gcagacttca taaatgtatg cccatctaaa ttttctccaa ctatggctac aaatgcattt
    34081 tgggcatttg cctccctgct atctataact atatcactta cacagtcatt ataatctcca
    34141 tatattaact tcccattagt tgcaagtact aactctttta ttgttaagca ctccattatt
    34201 aaactcctcc tttaaaacct tagctatatt ctcttagcaa caaaaggcaa tgtataccgt
    34261 tatatataca ttgcctacga taactcttaa ctttataatt atatactata tttctgtttt
    34321 tttgaagatg tttatgggta tatgttaatt atctgttact ttttagtttt caaaatatag
    34381 ggttatactt gagtcttcat taacttttac tccaggtttt gggaatatat cctttacctt
    34441 tgtatcttct ggaagttcta tatctgcatc taagtctggt tttaacttcg catcctccaa
    34501 tgcctttaca gcatctccta tttttagatt tcttacatct ggcactttaa cttgcttctt
    34561 ttcatactca gctttttctt cttctttata tactggttta actcccaaat attttaaaga
    34621 gtcattcatt atttctttca ctattggtcc tgctgtagta ctaccaaatg cacttactcc
    34681 tgttggttcg tcaacaatag caagaactac tatttgcggg tcatcacaag gtgctattcc
    34741 aacaaatgaa catatatact ttcctggagc atacttacca tctataactt tttgagctgt
    34801 acctgtttta ccaccaagtc tataacctgg tatatacgct atctttcctc caccttcagt
    34861 tacaactgat tctgcaattt ctaacatttt tttagatgtt tctttagata taacacttct
    34921 tacttttttt ggttttactg tttcagttat atttccttta ttgtcagtat aagattttac
    34981 cactcttggt tgcattaaat ctccaccatt agctattgat gatatagctg ttataagttg
    35041 tattggagtt actgatatag attgaccaaa tgatattgta gccaattcaa ctggtcctac
    35101 atttttttcg ttatacagta ttcccttagc ttctcctggt aaatctatac ctgtcttatc
    35161 cataagacca aatgattcta tataatcata catttttcca acacctaatc tgcttcctaa
    35221 ctcaacaaat actgggttgc aagagttttg tactgcttgt ttaaattctt gtgttccatg
    35281 aggtctgtaa tgtctccaac atttaatctt tcttcctcca acagttacac tacctgtaca
    35341 tgtgaatttt tctccatctt taattactcc ttcttcaaga gcactggaag atgtaataag
    35401 cttaaatgta gaacctggtt catatgtatc acttactgct ggatttctcc acatttgata
    35461 atagcctttt attttatctt tatcattata tttttcaagt tcttcttgat aatatggata
    35521 tattggcgtc cttgagtcat tagggtcata atctggtttt gatgctaaag caagtatatc
    35581 acctgtttta gggttcatag ctattgcagt aacctttttt gcattattta actcataagc
    35641 tttctgtact gctttttcag tatagtgttg aattacttca tctatagaca acacaagacc
    35701 attaccttgt acaggctcat aatatttttc cattccttgt ggtatttctc ttcctgaagc
    35761 atcagtactt actataagtt ttccaggctt tccttttaac tttttatcat attgcatttc
    35821 tacaccagat atacctgttg catctgaaga agtatgtcct aacacatatg gtgcaaaatt
    35881 cccatatggg taatatcttt gattatcttc tgccacccat attccactta atttagcatc
    35941 tcttatttga cttgccttat catcatctat ccatcttttt acttttacaa gagccatatt
    36001 ttttttgctt attaaagcgt aaatatcttt ataatcttca tccaaaatct cagctacctt
    36061 ttcggctgct tcttttttat cttctacttc cacaggttta caccaaacag tatacttagt
    36121 tacacttact gctaactctt tcatatttct atcatatata gttcctcttt tgggttctat
    36181 tggtatatct cttgtttgtt gttctagtgc ttttgtactc aaccaatttc ccttcataag
    36241 ttgtagatac ccagtcctaa taacaaggca aaaaaacaat gcacatgcta gaataagaac
    36301 gagtaccagc cttttcttac ttatcctctt tacttttctc aaatctattc cccctagtta
    36361 tccacatcta tatactttat ttgtccatct tttggatagt ccatattgag caattcctta
    36421 gcctttagct caatttcttt tattgatgtt ttttcagttt cttttgcctt tagttcctct
    36481 aatactatct ctttttttct taaatctttt tgtaaataat agatatcgta cttcatttcc
    36541 gagatttttg aataaccaca tagattcaat attattactg aaaaagttgc aatcagtaaa
    36601 atgcatttag ctcctatttt tttcttattt ctctttttat aattattctt ttttttattt
    36661 ttatattcat ttagattatg tattttataa ttttttttca aattctcacc ccgatagtat
    36721 at
//
LOCUS       DAELTA010000019        36664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.019, whole genome shotgun sequence.
ACCESSION   DAELTA010000019 DAELTA010000000
VERSION     DAELTA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.019"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            341..1477
                     /locus_tag="KPG13_000829"
     CDS             341..1477
                     /locus_tag="KPG13_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG0984573.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            1536..2318
                     /locus_tag="KPG13_000830"
     CDS             1536..2318
                     /locus_tag="KPG13_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0984574.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            2337..3347
                     /locus_tag="KPG13_000831"
     CDS             2337..3347
                     /locus_tag="KPG13_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0984575.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            3366..4163
                     /locus_tag="KPG13_000832"
     CDS             3366..4163
                     /locus_tag="KPG13_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0984576.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            4261..5106
                     /locus_tag="KPG13_000833"
     CDS             4261..5106
                     /locus_tag="KPG13_000833"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG0984577.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            5193..6368
                     /locus_tag="KPG13_000834"
     CDS             5193..6368
                     /locus_tag="KPG13_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0984578.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            6563..7465
                     /locus_tag="KPG13_000835"
     CDS             6563..7465
                     /locus_tag="KPG13_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0984579.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            7479..8483
                     /locus_tag="KPG13_000836"
     CDS             7479..8483
                     /locus_tag="KPG13_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG0984580.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(8627..8854)
                     /gene="acpP"
                     /locus_tag="KPG13_000837"
     CDS             complement(8627..8854)
                     /gene="acpP"
                     /locus_tag="KPG13_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0984581.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            9026..9451
                     /locus_tag="KPG13_000838"
     CDS             9026..9451
                     /locus_tag="KPG13_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984582.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            9499..9705
                     /locus_tag="KPG13_000839"
     CDS             9499..9705
                     /locus_tag="KPG13_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984583.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            9887..10066
                     /locus_tag="KPG13_000840"
     CDS             9887..10066
                     /locus_tag="KPG13_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984584.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            10100..10300
                     /locus_tag="KPG13_000841"
     CDS             10100..10300
                     /locus_tag="KPG13_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984585.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            10553..11569
                     /gene="ccpA"
                     /locus_tag="KPG13_000842"
     CDS             10553..11569
                     /gene="ccpA"
                     /locus_tag="KPG13_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG0984586.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(11692..12090)
                     /locus_tag="KPG13_000843"
     CDS             complement(11692..12090)
                     /locus_tag="KPG13_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984587.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            12302..13213
                     /locus_tag="KPG13_000844"
     CDS             12302..13213
                     /locus_tag="KPG13_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984588.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            13328..13508
                     /gene="ssrS"
                     /locus_tag="KPG13_000845"
     ncRNA           13328..13508
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KPG13_000845"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13691..14908
                     /gene="cdeC"
                     /locus_tag="KPG13_000846"
     CDS             13691..14908
                     /gene="cdeC"
                     /locus_tag="KPG13_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG0984589.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(15009..16331)
                     /locus_tag="KPG13_000847"
     CDS             complement(15009..16331)
                     /locus_tag="KPG13_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0984590.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(16382..17176)
                     /locus_tag="KPG13_000848"
     CDS             complement(16382..17176)
                     /locus_tag="KPG13_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0984591.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            17286..18731
                     /locus_tag="KPG13_000849"
     CDS             17286..18731
                     /locus_tag="KPG13_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0984592.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            18760..18969
                     /locus_tag="KPG13_000850"
     CDS             18760..18969
                     /locus_tag="KPG13_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG0984593.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            18989..20383
                     /locus_tag="KPG13_000851"
     CDS             18989..20383
                     /locus_tag="KPG13_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0984594.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            20623..21375
                     /locus_tag="KPG13_000852"
     CDS             20623..21375
                     /locus_tag="KPG13_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0984595.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            21401..21814
                     /locus_tag="KPG13_000853"
     CDS             21401..21814
                     /locus_tag="KPG13_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0984596.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            21818..22561
                     /locus_tag="KPG13_000854"
     CDS             21818..22561
                     /locus_tag="KPG13_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG0984597.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            22561..23031
                     /locus_tag="KPG13_000855"
     CDS             22561..23031
                     /locus_tag="KPG13_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984598.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELKMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            23048..23806
                     /locus_tag="KPG13_000856"
     CDS             23048..23806
                     /locus_tag="KPG13_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0984599.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            23790..24632
                     /locus_tag="KPG13_000857"
     CDS             23790..24632
                     /locus_tag="KPG13_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0984600.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(24792..25697)
                     /locus_tag="KPG13_000858"
     CDS             complement(24792..25697)
                     /locus_tag="KPG13_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0984601.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            26059..26793
                     /locus_tag="KPG13_000859"
     CDS             26059..26793
                     /locus_tag="KPG13_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984602.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            27004..28185
                     /locus_tag="KPG13_000860"
     CDS             27004..28185
                     /locus_tag="KPG13_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG0984603.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            28169..28741
                     /gene="mobB"
                     /locus_tag="KPG13_000861"
     CDS             28169..28741
                     /gene="mobB"
                     /locus_tag="KPG13_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG0984604.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            28860..29135
                     /locus_tag="KPG13_000862"
     CDS             28860..29135
                     /locus_tag="KPG13_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984605.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            29378..31078
                     /locus_tag="KPG13_000863"
     CDS             29378..31078
                     /locus_tag="KPG13_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984606.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            31441..32628
                     /locus_tag="KPG13_000864"
     CDS             31441..32628
                     /locus_tag="KPG13_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984607.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            32688..33851
                     /locus_tag="KPG13_000865"
     CDS             32688..33851
                     /locus_tag="KPG13_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0984608.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            34002..34805
                     /gene="zupT"
                     /locus_tag="KPG13_000866"
     CDS             34002..34805
                     /gene="zupT"
                     /locus_tag="KPG13_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG0984609.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            34900..35460
                     /locus_tag="KPG13_000867"
     CDS             34900..35460
                     /locus_tag="KPG13_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG0984610.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            35710..36423
                     /gene="rgbR"
                     /locus_tag="KPG13_000868"
     CDS             35710..36423
                     /gene="rgbR"
                     /locus_tag="KPG13_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG0984611.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT        14396 a         3929 c         6628 g        11711 t
ORIGIN      
        1 cagatgtaca gacaatattt aatgtttaat ataccttaat ataaatatcg taaacaaatt
       61 attttatctc taaccagtgt gaaagaaatc ttattctaac ttgaaatttc acaaaattcc
      121 aaaaagcgag atttttttta agtatagttg attttaaatt aacaatttat gattaaatta
      181 atgtcataat tggttagtat aatcacatat aaatatagag cgtagtttga ttaaggttat
      241 aaatttctcg acgtatattc aaatacctta ttatagatgt tgccgagatt ttaattaata
      301 aatgaaaata aaatatttat aaaatactga ggaggctatt atggatttaa attctaaaaa
      361 atatcagatg cttaaagagc tatatgtaag cttcgctgaa aatgaagtta aacctttagc
      421 aacagaactt gatgaagaag aaagatttcc ttatgaaaca gtggaaaaaa tggcaaaagc
      481 aggaatgatg ggtataccat atccaaaaga atatggtgga gaaggtggag acactgtagg
      541 atatataatg gcagttgaag aattgtctag agtttgtggt actacaggag ttatattatc
      601 agctcataca tctcttggct catggcctat atatcaatat ggtaatgaag aacaaaaaca
      661 aaaattctta agaccattag caagtggaga aaaattagga gcatttggtc ttactgagcc
      721 taatgctggt acagatgcgt ctggccaaca aacaactgct gttttagacg gggatgaata
      781 catacttaat ggctcaaaaa tatttataac aaatgcaata gctggtgaca tatatgtagt
      841 aatggcaatg actgataaat ctaaggggaa caaaggaata tcagcattta tagttgaaaa
      901 aggaactcct gggtttagct ttggagttaa agaaaagaaa atgggtataa gaggttcagc
      961 tacgagtgaa ttaatatttg aggattgcag aatacctaaa gaaaatttac ttggaaaaga
     1021 aggtcaagga tttaagatag caatgtctac tcttgatggt ggtagaattg gtatagctgc
     1081 acaagcttta ggtttagcac aaggtgctct tgatgaaact gttaaatatg taaaagaaag
     1141 agtacaattt ggtagaccat tatcaaaatt ccaaaataca caattccaat tagctgatat
     1201 ggaagttaag gtacaagcgg ctagacacct tgtatatcaa gcagctataa ataaagactt
     1261 aggaaaacct tatggagtag aagcagcaat ggcaaaatta tttgcagctg aaacagctat
     1321 ggaagttact acaaaagctg tacaacttca tggaggatat ggatacactc gtgactatcc
     1381 agtagaaaga atgatgagag atgctaagat aactgaaata tatgaaggaa ctagtgaagt
     1441 tcaaagaatg gttatttcag gaaaattatt aaaatagata aatattaaaa tatacacaat
     1501 aggtcgatat taaaatagat atggaggaag gatttatgaa tatagtcgtt tgtataaaac
     1561 aagttccaga tacaacagaa gttaaactag atcctaatac aggtacttta attagagatg
     1621 gagtaccaag tataataaac cctgatgata aagcaggttt agaagaagct ataaaattaa
     1681 aagaagaaat gggtgctcat gtaactgtta taacaatggg acctcctcaa gcagatatgg
     1741 ctttaaaaga agctttagca atgggtgcag atagaggtat attattaaca gatagagcat
     1801 ttgcaggtgc tgatacttgg gcaacttcat cagcattagc aggagcatta aaaaatatag
     1861 attttgatat tataatagct ggaagacagg cgatagatgg agatactgca caagttggac
     1921 ctcaaatagc tgaacattta aatcttccat caataacata tgctgaagaa ataaaaactg
     1981 aaggtgaata tgtattagta aaaagacaat ttgaagattg ttgccatgac ttaaaagtta
     2041 aaatgccatg ccttataaca actcttaaag atatgaacac accaagatac atgaaagttg
     2101 gaagaatata tgatgctttc gaaaatgatg tagtagaaac atggactgta aaagatatag
     2161 aagttgaccc ttctaattta ggtcttaaag gttctccaac tagtgtattt aaatcattta
     2221 caaaatcagt taaaccagct ggtacaatat acaatgaaga tgcgaaaaca tcagctggaa
     2281 ttatcataga taaattaaaa gagaagtata tcatataatt taagggggga taaagaatgg
     2341 gtaacgtttt agtagtaata gaacaaagag aaaatgtaat tcaaactgtt tctttagaat
     2401 tactaggaaa ggctacagaa atagcaaaag attatgatac aaaagtttct gcattacttt
     2461 taggtagtaa ggtagaaggt ttaatagata cattagcaca ctatggtgca gatgaggtaa
     2521 tagtagtaga tgatgaagct ttagcagtgt atacaactga accatataca aaagcagctt
     2581 atgaagcaat aaaagcagct gaccctatag ttgtattatt tggtgcaact tcaataggta
     2641 gagatttagc acctagagtt tctgctagaa tacatacagg tcttactgct gactgtacag
     2701 gtcttgcagt agctgaagat acaaaattat tattaatgac aagacctgcc tttggtggaa
     2761 atataatggc aacaatagtt tgtaaagatt tcagacctca aatgtctaca gttagaccag
     2821 gggttatgaa gaaaaatgaa cctgatgaaa ctaaagaagc tgtaattaac cgtttcaagg
     2881 tagaatttaa tgatgctgat aaattagttc aagttgtaca agtaataaaa gaagctaaaa
     2941 aacaagttaa aatagaagat gctaagatat tagtttctgc tggacgtgga atgggtggaa
     3001 aagaaaactt agacatactt tatgaattag ctgaaattat aggtggagaa gtttctggtt
     3061 ctcgtgccac tatagatgca ggttggttag ataaagcaag acaagttggt caaactggta
     3121 aaactgtaag accagacctt tatatagcat gtggtatatc tggagcaata caacatatag
     3181 ctggtatgga agatgctgag tttatagttg ctataaataa aaatccagaa gctccaatat
     3241 ttaaatatgc tgatgttggt atagttggag atgttcataa agtgcttcca gaacttatca
     3301 gtcagttaag tgttgcaaaa gaaaaaggtg aagttttagc taactaattt cttggggagg
     3361 gtattatgag tacaagtgat gttaaagttt atgagaatgt agctgttgaa gtagatggaa
     3421 atatatgtac agtgaaaatg aatagaccta aagcccttaa tgcaataaat tcaaagactt
     3481 tagaagaact ttatgaagta tttgtagata ttaataatga tgaaactatt gatgttgtaa
     3541 tattgacagg ggaaggaaag gcatttgtag ctggagcaga tattgcatac atgaaagatt
     3601 tagatgctgt agctgctaaa gattttagta tcttaggagc aaaagctttt ggagaaatag
     3661 aaaatagtaa aaaagtagtg atagctgctg taaacggatt tgctttaggt ggaggatgtg
     3721 aacttgcaat ggcatgtgat ataagaattg catctgctaa agctaaattt ggtcagccag
     3781 aagtaactct tggaataact ccaggatatg gaggaactca aagacttaca agattggttg
     3841 gaatggcaaa agcaaaagaa ttaatcttta caggtcaagt tataaaagct gatgaagctg
     3901 aaaaaatagg gctagtaaat agagtcgttg agccagacat tttaatagaa gaagttgaga
     3961 aattagctaa gataatagct aaaaatgctc agcttgcagt tagatactct aaagaagcaa
     4021 tacaacttgg tgctcaaact gatataaata ctggaataga tatagaatct aatttatttg
     4081 gtctttgttt ttcaactaaa gaccaaaaag aaggaatgtc agctttcgtt gaaaagagag
     4141 aagctaactt tataaaaggg taaaaatcta attaaaaata tatttgctgt taactagatt
     4201 aaatagtatt ttaatctagt taacagaata taaatactaa aataacagga ggttataact
     4261 atgaaattag ctgtaatagg tagtggaact atgggaagtg gtattgtaca aacttttgca
     4321 agttgtggac atgatgtatg tttaaagagt agaactcaag gtgctataga taaatgttta
     4381 gctttattag ataaaaattt aactaagtta gttactaagg gaaaaatgga tgaagctaca
     4441 aaagcagaaa tattaagtca tgttagttca actactaact atgaagattt aaaagatatg
     4501 gatttaataa tagaagcatc tgtagaagac atgaatataa agaaagatgt tttcaagtta
     4561 ctagatgaat tatgtaaaga agatactatc ttggcaacaa atacttcatc attatctata
     4621 acagaaatag cttcttctac taagagacca gataaagtta taggaatgca tttctttaat
     4681 ccagttccta tgatgaaatt agttgaagtt ataagtggtc agttaacatc aaaagttact
     4741 tttgatacag tatttgaatt atctaagagt atcaataaag taccagtaga tgtatctgaa
     4801 tctcctggat ttgtagtaaa tagaatactt atacctatga taaatgaagc tgttggtata
     4861 tatgcagatg gtgttgcaag taaagaagaa atagatgaag ctatgaaatt aggagcaaac
     4921 catccaatgg gaccactagc attaggtgat ttaatcggat tagatgttgt tttagctata
     4981 atgaacgttt tatatactga atttggagat actaaatata gacctcatcc acttttagct
     5041 aaaatggtta gagctaatca attaggaaga aaaactaaga taggattcta tgattataat
     5101 aaataagaat ttgggaatta aaagtttaaa taaaattgtt taaaaaacaa tttcggatat
     5161 atgaaaaatc taatttaatg ggggtaatga atatgagaga agtagtaatt gccagtgcag
     5221 ctagaacagc agtaggaagt tttggaggag catttaaatc agtttcagcg gtagagttag
     5281 gggtaacagc agctaaagaa gctataaaaa gagctaacat aactccagat atgatagatg
     5341 aatctctttt agggggagta cttacagcag gtcttggaca aaatatagca agacaaatag
     5401 cattaggagc aggaatacca gtagaaaaac cagctatgac tataaatata gtttgtggtt
     5461 ctggattaag atctgtttca atggcatctc aacttatagc attaggtgat gctgatataa
     5521 tgttagttgg tggagctgaa aacatgagta tgtctcctta tttagtacca agtgcgagat
     5581 atggtgcaag aatgggtgat gctgcttttg ttgattcaat gataaaagat ggattatcag
     5641 acatatttaa taactatcac atgggtatta ctgctgaaaa catagcagag caatggaata
     5701 taactagaga agaacaagat gaattagctc ttgcaagtca aaataaagct gaaaaagctc
     5761 aagctgaagg aaaatttgat gaagaaatag ttcctgttgt tataaaagga agaaaaggtg
     5821 acactgtagt agataaagat gaatatatta agcctggcac tacaatggag aaacttgcta
     5881 agttaagacc tgcatttaaa aaagatggaa cagttactgc tggtaatgca tcaggaataa
     5941 atgatggtgc tgctatgcta gtagtaatgg ctaaagaaaa agctgaagaa ctaggaatag
     6001 agcctcttgc aactatagtt tcttatggaa cagctggtgt tgaccctaaa ataatgggat
     6061 atggaccagt tccagcaact aaaaaagctt tagaagctgc taatatgact attgaagata
     6121 tagatttagt tgaagctaat gaggcatttg ctgcccaatc tgtagctgta ataagagact
     6181 taaatataga tatgaataaa gttaatgtta atggtggagc aatagctata ggacatccaa
     6241 taggatgctc aggagcaaga atacttacta cacttttata tgaaatgaag agaagagatg
     6301 ctaaaactgg tcttgctaca ctttgtatag gtggaggaat gggaactact ttaatagtta
     6361 agagatagtt ttagattata atatttacaa attaaatatt taattatgaa tttaacctgt
     6421 aagaataact atttgttatt cttacaggtt tttatagtta aaaataatta ttatctatta
     6481 aacaaaaata gtgtaaaata tataatatca tgattttgaa gatataaaaa gtaggaagat
     6541 ataaaagaaa gtaggtaaaa gtttgtttaa aaaagagaat caaagatata atcttatatc
     6601 gtatacaaat gaagaagaaa tgactttaaa agaagtttta ttggacaagc taaatttctc
     6661 agttaggtca ttatcaaaaa tgaagaggga aaagagtgtt ttagtaaatg gagtatataa
     6721 aaaaccgagt ttaaaggtgt atagtggtga tttaatagaa gtaaaaatag acgaggaaaa
     6781 agcaaatttt gaacctcaag atttaaattt acagataatt tatgatgatt ttgatataat
     6841 tatggtgaat aaacctccgt ttatggtagt tcacccaaca aaaagccact atgataaaac
     6901 aatagcaaat ggtattagtt actatataga taatcaaaaa gaaaacgtaa aaataagatt
     6961 tgtaaatagg ttagatatga atacttcagg gttagttata gtagcaaaaa atgcttatgc
     7021 acatcatact ttgtctacag caatgagtga aaataaagtt gaaaaaaagt atataacagt
     7081 agttgatgga attataaaag aaaatgaggg aactatagat gagccaattt atagacctac
     7141 agaagattca ataaagagga taatagatga aagaggacag tcatctgtta ctcactataa
     7201 agtaatagaa agactagaaa atgcaactgt attagaagta agcttagaaa ctggtagaac
     7261 acatcaaata agagtacata tggctcatat aggtcatgga ataataggtg atgagttgta
     7321 tggttatgta gatgaagagc taataaatag gcaagcactt catgcatata agctagagtt
     7381 tgaacaacca agaacaaaag aaaaattgaa atttaaagca gatataccag aggatatgaa
     7441 agagttaata tcgaaattaa gataatgggg tgatatatat gataatacat aaatttataa
     7501 tacatgtttt ggacaaaaat agtgatgttc caatattaaa tgactttgaa ggcaaagtta
     7561 atcaagaagt ggatggtttt ttccagaaag ctataaaaag aattgcaaaa gatgaggatt
     7621 taagaaaagg tgtttttaaa gattataatg acaatttaat taaaaactgt tgtgaacaaa
     7681 taatatatga tgaaagtact tttttgaaaa attctaaaga aattgcatct tacttgtttg
     7741 acgttatgaa aattaatgct gcactagaat cttgtgattt ggcaatttgt ttatacacta
     7801 ttaaagatga aaagagtgta gcaatactga aattagatta taaaaagttg tacactcatt
     7861 caattgaatt tgtggatgat aaatttaata ttcaaatggt ttcaaatgaa attggtatcc
     7921 cagagacttt aagacaaaag caaggagctt tgattagtct aagtggtata aatgatgagt
     7981 ttcatctaag gcttttagat aaggatgctg aaaaagaagg gtcagaatct aagtttgtaa
     8041 cagaattttt aaatgctaaa aaaatagatg atgataagta caagaccaag gtatttaaaa
     8101 atacagctga aaattggata accaatgctc ttagtaatga tataaagcaa gctgaagatg
     8161 taagaagtat attaaattat acactgaagg aaaaacatga agttgatata aatgactttg
     8221 ttgataattc aattaaagat gatgagttaa aggatagctt taaagagcat atggaagaaa
     8281 aaggtcttga cgaaagtttt agtatagata aaaaatgggt tgagaaaaag cttaaaaaaa
     8341 gaagtataaa aacggataat ggttttgaca taaaagggaa tctaactgat tttgaagacc
     8401 caatgaagta tactgtaaaa caaaatcaag atggaactat agatataata ataaagaatg
     8461 ttacgtttta tgaagaaaag taatttatat acttagtata taaattaaat ataagagcgt
     8521 gtattttata tattgtaaat aaatgtaatc tagtctgtgc gaactctaat ataatttatt
     8581 atgtagaagt ttacacagac tatttatatt tgtagacaaa acgatactat ttgttttctt
     8641 cgatatattt acatatatca ccaatacatt taaagttctc agcttcctcg tctggtattt
     8701 ctattccaaa ttcatcttca agggccatta taatttcaac agcatctaat gaatcagctt
     8761 ctaagtcgtc cattaaagat gcttccatag ttatctcatc taaattgtct acccctaatt
     8821 gttctgctat tatttctttt attttctcaa acattttaag cctcctaaag cagttttaat
     8881 taattttaat gttagtttaa tataatatca agaatttttc aatatggaat ctataaaaaa
     8941 taataaaatt ttataaatgt agtaataaag tattatttga tgaataaact ttattgataa
     9001 caaatataac ttaggaggaa atgctatgaa atatgtagga ctattactaa cttcagtagg
     9061 aatgttttta cttatagcag taaattttta ttataattca ataactttag atatgcaacg
     9121 tatagaggat tatgtaatgg agaccaattt aatccttgaa gatgtagctg aaaaggagag
     9181 ttatgtatct aatgaaaaag aagactacat atcaaggtta atgcatgtaa agaaaggaat
     9241 agaaaattca aaaacatcct tcttgataga aagatataag gagtacaaaa taaaatcaat
     9301 agaaagttta atctatacta tatcagagga gaaaaaagat tatctggatg aagttgatag
     9361 atacaataaa cttggtgaaa aagaaataaa taaactaatc aataaaaact ttttagaagt
     9421 aacttatctg tctattacta catatattta attaaagaga caaaacttag tctcttaatg
     9481 attcaaggag gattagttat gacaaaatta gaagacaata tggaacgttt ctttggagga
     9541 tgcgaaggat tttttgggaa taaaatgttg ataataatag ttatagtatt cttactatta
     9601 tgcacagaca ttttagaaga cttattatgc gatgacaata tatggatatg ggttatatta
     9661 atagtattgt tattatttaa cttcgatgat ggttgttgtt gctaaaaaat attgattatt
     9721 aaataggtaa aaccagtaac ttatacaagt tatggtttta ccttttttag ttcaaattaa
     9781 aaaatttaaa aaaatagtaa aaatacatta gcaaaatgga aaccaaatgc atagtatatg
     9841 ttaggagcga aaagctcaac aaaatatcaa caggaggtaa cgtataatgt tagatagatt
     9901 ctttggagaa gaaggatgtt ttggtggagg aggatggtgg ataatagtac tattcttcct
     9961 attcttagct tttggagata gctgggcaga atttgacata atggcatgga taccattcct
    10021 aatagtttta ttaatacttt gctgctgtgg tggatgctta gaataagaac acagttatta
    10081 aaaaaaggag ggctttgtta tgttagatag attttttgga gaagaaggat gctttggtgg
    10141 aggaggatgg tggataatag tactattctt cctattctta gcttttgaag attgctggag
    10201 agacattgac ataatgtcat ggataccttt cttaatatta ttgctaataa cttgttcatg
    10261 tggtggattc tttgatggtg aagtaggttg cggctgctaa tacttaaatc attagaataa
    10321 aaaaatgctg gtattttata ccagcatttt tttattttat tttttaatat gatttaaatt
    10381 aagaatgaat attattaaat ttgttgaata tagggtataa tataaaatat aatgtattta
    10441 aatgtacttg cttttatggt aaaataaaaa cttatataaa tgtagttaaa ataggaacct
    10501 tcaaaaattt ggttcagttt tagataaatt tagtaaaata aggaggatga agatgaaagg
    10561 caatataacg ataaaagatg ttgctaaaca agcaggagtg tcaatatcta ctgtatctag
    10621 agttataaat gattcaaaac ctgtaactga tgaagtcaaa caaaaagttt tagaggttat
    10681 aaaagagact ggatatatac caaatccact tgctagaagc ttagtaacaa agaagagtca
    10741 attaataggg gtaatagttc cagaagtttc agattctttt gttaatgagg tgttaaatgg
    10801 gatagaagag gttgctaaaa tgtatgacta tgatattctt ttagcgaata catactctga
    10861 taaggaacaa gaacttaaga gtataaatct attgagagca aaacaagtgg aaggtatagt
    10921 tatgatttca tggatagttg aacaagaaca tatcaactat atacaaaatt gtggaatacc
    10981 agcgacatat ataagtaaaa ctgctagaaa ttatgatata tatacagtaa gtactagcaa
    11041 cgaagaagct acttttgata tgacagagca tcttataaag aaaggtcatg aaaagatagc
    11101 ttttataatg acgagtaaag atgatactgt tttagaaatg gaaagacttg ctggttatga
    11161 gaaagcactt tcaaataaca atatagaatt agacaagagt ttgattaagt atggtggaac
    11221 tgattatgag agtggataca atagtatgaa agaactatta gatgatggaa taatacctca
    11281 tgcggctttt gtaacaggtg atgaggctgc cataggtgct ataaatgcta tatgtgatgc
    11341 tggatataag gttccagaag acatatctgt tgcaggattt aatgatgtta agatagctag
    11401 aatgtataga cctaaactta ctacagtata tcaacctcta tacgatatgg gagcagtagc
    11461 aataagaatg gttataaagt taataaataa ggaattaatt gagaataaga aaatagaatt
    11521 accttataga attgttgata gagaaagtgt tacagaaaga aaaaaataaa actattaaaa
    11581 tcaatcaggt agtgcctaag gcactacctg attttttatg tcaataatat caaatttaat
    11641 aatagattaa ctaaaaataa ttatctaatt acttaaatag tttattttta tctattcatg
    11701 agggttagac tttttagttt tcttaaataa acttttagat tttttttgtt gactacctgg
    11761 tgtatttttt ggtgcaggtg aatttgactt acctttagta ggtttagcct tcttagtttt
    11821 agctggttta gtatctcttt ggttttctgc atctgagatt ttttccatta tattatccag
    11881 ttttttgcgt tgattttcat ctaaaaatgc tttgaatttt gatactactt catctctgcc
    11941 atctaggttc ttacctattt ttataagttc ctccataagt tcattttctg attttccttt
    12001 gtaactatca gccatctttt ctattttatc cttgtcaata tttttttgtt tagccatttt
    12061 ttctaatgac tctgtatcta attttttcat aaaaaaaccc ctttctataa atatatattg
    12121 aattatattc atactattta tatatagaaa taatattgaa gtggtgaatt gtgagtgata
    12181 tataatataa ataaaaattc ttatagataa tatatatgca ctgagcttta aaaatggaac
    12241 attaaaatat gaaaaacata ctttaatata gggcaattat ataatataag ggggaattgt
    12301 cttgaataac ttactatttt tgattggagt gattgcattg agtaatggta atatctctat
    12361 atttgatgat aaaaataaat caaaaggaaa taatagagtt agaaaaaaca aaaaaaatgc
    12421 aaatgataaa ctttcacagt ctacacatgg caatttaagg tttgatgcaa atgatataaa
    12481 aagaggttta aggctcatgg atttaagtga agaagattta gaaatgggaa tggaaataat
    12541 aacaagaact aaaaaataca tgtcaagaga tgaaagaaaa atactaataa aaatagaaag
    12601 tgttttagat ttagttagag gaataaaaaa attaaataat atagatatgg tagatacaga
    12661 agatgaaacg gattttttca gaaaaatgga tagtgatgat aaaaagaata tgatgataaa
    12721 ggaaattata gatgtattcc cagaaaaaag aaaaaattca gttgaaaaag ctataggaat
    12781 gaaaaagaag atagatttat ttgcagaatt atttttacca gatgactttg gtgaaggtgg
    12841 atttagtttg agctctttgg caaatataaa caatcttggt agtatgaata atctcaaatt
    12901 gttaggaagt cttttaagag gtgatgatag taataatgat gatgaagaca ataatgatga
    12961 gttaaattat gaagacgaag aattagattg tgaagacaaa gaattggatg atgattatga
    13021 tgagttaaca gacgaaggtg aaatggagga taatgaagat gaattagtag aagataagaa
    13081 cacaggctat gaaaatatag aaaatgatga agatatagta tatgatgagg aactatacaa
    13141 cattaatgag caagaatccg aatacaatta taatgaaaaa aatactaaaa ctagagctaa
    13201 gaaaaaacga taattaacta aatacataaa gaaaaaagga agaaaaagta atgatagaat
    13261 aagattatct caattactat ataaaaccac tttaaaaaat tcaaaattta ttatataata
    13321 ataatatgaa atcctgaaac atacgataag gcttttttaa attcaaccta caaatgaaat
    13381 acgggagacc gtgtctggta aattataaca tgcctagtta gtttggacgt taaaggttac
    13441 tggcaagtca cccacctggg agtgcctagg gtgtgaattt acttagccac cggtgtttcg
    13501 ggacaaaaaa agtgctacta tttcaaatag tagtactttt tttattaata tgcatttttc
    13561 tactaaatac gcctaattat tgataaattc aaaaagcaag taacaattaa gattataaaa
    13621 gcatagtatt aattaggctt caaaaagcca aagacttaat aagaatcgct tacagggagg
    13681 gcaatgaaaa atgcaagatt ataaaaaaaa taaaagaaga atgatgaatc agccaatgtc
    13741 tacaatgaat gaagaagaag tgtatacaga tgaaataaat tcagaagaca tgagaggttt
    13801 taaaaaatca caccatcata atggatgtaa tactgataat aagtgtgagt gccatgatga
    13861 ttgcaatcca tgcaacccat gtaatccatg taaacctaac ccatgcaatc catgcaaacc
    13921 taatccatgt gatgacaatt gtggatgcca tgacaattgt aaatgtgatt gtgaaccatg
    13981 tgaaatggat tcagatgaat gttttgaaaa caaatgtgga ccagaatgct gtaatcctat
    14041 atctccaaga aacttctctg tatcaaatgc agtgccattt gcaatagagg ctaatagaat
    14101 atttgatact atgcaattcc aaacatttac agatgcaaca ggaccaaatg gagagccatt
    14161 aacttttgaa acagaagtag tagaagtatt tggttcagtt ccaagtgcag gtcaagcaag
    14221 tgtaactata gaaaaaatat gcttaagtaa tgatggaatc gttatagaca caggaatgac
    14281 aactttagaa gatttcgatt tagacccatt aggagatata gtaggaagaa actgtgaaac
    14341 aacttttgaa tttgcagttt gtggagaaag aaacgctgag tgctgtagac aaggaaaagg
    14401 caaatcagta gcttataaac aaagaggatt aactgtagca gttcgtaatt tagtactaga
    14461 gctaagaggt agatgtggat gtacagagtt cgttgcatta gctttcccag cagttagagc
    14521 aggaggtgga tgtaagagaa gagttgatta tgtagaattt acttttaaca cactttcagc
    14581 accaatatgc ttgccagctg acggaagagc tgttacttta agacaagaat atcaaactaa
    14641 cttaactgta gattgtatag gaaaatctat attaaaatta gaatgcaacg aatgttgtga
    14701 acctttctat gaattaatta taccaaatga tatagattta gtactttgct tacaagaaac
    14761 agttagcaca ttaataagtg aacaaatagt agttttagca tcaccaaatc caatccaacc
    14821 aagacttgtt gatactttct ctaaagtatg tgatttttcg caatgtggac ctaatcatgg
    14881 aagtggaaag ccaagttgcc acagatagat aggtaagata tatagacagt ttaaattata
    14941 aataaaaatc atctaaggaa ggctaccaaa gtgtataatt tacattggta gccttcacgt
    15001 ttttataact agcgtataga ttttagtgat ttaaaaagca ctatagtatg taatagacaa
    15061 gtaaagattg atactatcgt cattgtgtat atgtatgcat aaatattaag tcctggaatt
    15121 ggaagaaata catataaagc tattagttga attaacatgc cagtaatagt atttatactt
    15181 gaagctaatt cttttcttat tgaatgtaaa atgccagata gagtttgatt tagggacata
    15241 aatagtggaa ctaaaaacat agcttttaga tattctcctg caagtgtatt tttaaatact
    15301 aatatacata aatcttttcc aaagaaataa aaaaatattg atgatagtat gcctacaaat
    15361 aaagtaagaa gtatggagta tttgattttt ttaattacac tcttacgctt tcttagagcc
    15421 atttcttgtg aaatagttgg tattaaattt acaataagcg ctgaaccaac agtaaaaggt
    15481 aaaaatataa aaggcattac catcccactg acaataccat acatacttaa agattgttga
    15541 taagtcattc ctgataatgc taatcttgat ggtatcatca tagaacttat agattgcaaa
    15601 agtatgtttg acattctatt acaagttatg ggaatagaca tttttaatgt ttccatagaa
    15661 gaattataaa aatcttttat gcttataata tacctattgt caaatgatga atctcgccat
    15721 aagcaaataa gcataaatat aatattactc aactcaccga ttgatatacc taatagagct
    15781 atataacaat tcatagattt attatttatg tacataacta gtaaaaaaac aaataatatt
    15841 ttaccaattt gttcaatgat ttgtccaata gcagggattt ttacattctt tatgccatag
    15901 taatatcctc ttagtacgtt tgaaagagtt attataacta tagcaggaca aacagctagt
    15961 ataaatagat ttaatttagc atcttttaaa aatttaagtg ataaaaaaga actattgaaa
    16021 gatgcaacta tagatataat tagtgctacg aaaaatgata tatataaagt tgatataaat
    16081 aaagcattag tattgtgtct atcgtttaaa gcttttctct ttgcaactag acaactaagt
    16141 gcagtaggta tacctgttgt tgtaacagct aagcaaatca tcaaaaagtt aaaaatcata
    16201 tgatagatac ctaaacctgt ttcacctagt gctcttgata agaatatttt atataaaaaa
    16261 ccaaatattc ttacaataaa attggatata aataaaataa ctgttgatag tactaaataa
    16321 ggtactttca aaaaaatcac ctccttatct tttaaatata ttcacaagga ggtgtaaaaa
    16381 tttataattt tataaattct ggtttcaatt tattaaattt agatacatat ttaaatccca
    16441 tattttttag taatgaatat atgtagtcaa actcacatgc aacttgactt atatgatgtg
    16501 aatctgaacc tgtggttatg atttcccctc ccaagtcttt atacatttga agtatatact
    16561 tagatggggt taggtctgga agattatatc tataacaaga ggtatttatc tcgatacctt
    16621 tgccatcata aatagcttgt ttaagagttt cttcaattat atcagaaaat agtctatcat
    16681 ctaaaatagt gtcataaggt gcataccttt taatcaaatc taaatgacct aagacagagt
    16741 aatctttata attcttaaca atattatata aataaagata gtaattttca taaacttcat
    16801 gttgagtttt atctttaaaa taattaccaa ggtataagtc cattgtatct atagcatgga
    16861 tagaacatat tataaagtcc aatggatatt gatgtgcttc ctttttgtat gtatccatta
    16921 attgagtttg aactccaaat tcaattcctt tttttattga tattttatct ttatatttgc
    16981 tttgaagagt ctctaaactc ttaaaataat cttcgtagac aattgaaaaa gaatcatcag
    17041 catatacatc atagtcaaca tggtctgtga aacaaatttc ctccaaaccg agttcaatag
    17101 atttttttac catttcttcc attgtagatt ttccgtctgt agagaaactt gagtgcatgt
    17161 gatagtcgta aaacataaat atttcccctt tttataaaaa ttttggtatt atatatacat
    17221 actaaataat atcaaatata cataaaagca tcaataaaat ataaatacaa tgagcggtga
    17281 taaatttgga aaaatacaga atagatttaa atgagaaaaa atcaactaaa tatatacaga
    17341 tatttaatca tataaaagaa ttgataataa ataaagagtt agtagaacat gaaaaattgc
    17401 ctccaataag aaaactatca aattttttac aagtaaataa cactactata gttaaggtat
    17461 acgagctttt agaaaatgaa ggatatgtat ataaaattat agggagtggt acttttgtat
    17521 caagtttaaa ttcttataaa agcggtatag ataacaggaa aaataagatt attgtgcaaa
    17581 gagaagattt aattcatttt gacaatggga atccatctaa tgatatgttt cctatcgatt
    17641 catttaaaaa tgctgtaaat atggcattat ctaaagatgg tagttcaata tttgaatatg
    17701 atgaaggtct tggctctgaa gaattaagag aaaaaatggt agagtatcta agtgatggaa
    17761 atataaaaac tactaaggaa aatattcaaa taatatcagg tgctcaacaa ggtatagata
    17821 tagtttgtaa aggacttata agttattcag atgttgtttt tatggaagaa ccttcttaca
    17881 atggagcaat tgaagtgttt aaaagtagag gtgcaaaaat aatatctata cctatgttag
    17941 atgatggaat tgatataggt attttaaaat taaagttgga aaaaataaaa ccaagactta
    18001 tatacattat gcctaatttt caaaatccaa ctggaatatc ttactcaaca tataaaaaaa
    18061 agaaattgat agaattggca gaagaacatg atttttatat aatagaagat gattttataa
    18121 gtgattttgt ttttgaatca aaggacaata gaacaattag aagttatgat gataaaaata
    18181 gagtagtata tataaaaagc ttttcaaaaa tacttatgcc aggtcttagg ataggaattg
    18241 tagaaatgcc aaatgagttg ttaaaaaaag ttttatgggc taaatattca tcagatatat
    18301 caacaccagg tttaattcaa aaatcaatgt attactatat gaataatttt gattggaaga
    18361 attatttgag tcatatagaa aagatatata ctgataagta taaattggca aaaaacttga
    18421 ttaatgacaa attgagtggt aaattaaaag ttagaaaatc ttgtggagga ataaactttt
    18481 ttttagaatt accaagagga tatacatctc aagcatttac aaattttatg ttaaacaaag
    18541 gagtgtcaat gcttccaggg acttattttt ttgacaatct agtggatgat agattcttta
    18601 ggataaatat agctcgccca agcatggaag aactagaaaa gggaatatct ataataagtg
    18661 ataatataga tgagtttttt tgtgaatata aaaacaagtt agatataaaa agcaataaat
    18721 tattttatta aataaataga ggttttttag gaggaaaaaa tgtttgacaa gaagaaatta
    18781 gataggatta atgaattagc aaagaaaaat aaagagggaa tcctttctgc tgatgaaatc
    18841 aaagaaagag aaattttaag gaaagaatat ttagaaaact ttagagccca ctttagatca
    18901 agactagata gtgtcaaagt agtttcacca gaagaatatg agcaatacat gaaaaacaat
    18961 aaaaattaga acttagtagg aggaaaatat gaacttaaag tacgaattta agaatgcttg
    19021 ggattttgaa agaaaagaaa atactattga acaaattatg caatattcta gcaattatat
    19081 ggagttttta agtaagtcaa agactgaaag actttcagta aaagaaatta taaaattggc
    19141 taaagaaaat aactatattt caatagatga agctatggaa aaaggaagca taagttgtgg
    19201 tgataagatt tatgttataa ataaagagaa ggcagtagca ctttttgtaa taggtaaaaa
    19261 ttatatagaa aaaggtatga agattatagg aagccatata gattcgccaa gattagattt
    19321 aaaaccaaat ccactgtatc aagagagtaa tctaggtttt tttaagactc attattatgg
    19381 tggaataaaa aaataccaat ggacggctat tccacttgct ttacatggta tagttatatt
    19441 aaatgatgga acaaaagtgg acatatctat tggggaagaa gatagtgacc cagtattttg
    19501 tgtaactgat ttattgattc atttggctgg agaccaaatg caaaagaaac tatcagaagg
    19561 aattagtgga gaagctttaa atatattaat tggaaatatg cctttagaag atgaagaaaa
    19621 agaacctata acagcaaata tattaaagat tttaaatgag aaatacaata tagttgaaga
    19681 agatttatta agtgctgaaa ttgaagtagt tccagctggt aaggctagag atttagggct
    19741 tgataggtct atggtgttgg gatatgggca tgatgatagg gtatgttcat atgctgctgt
    19801 aaaagctata ctagaaactg aacaaccaga atttacttca gttgcattat gtgtagataa
    19861 agaagaaatt ggttctaaag ggaatacagg tatgcactca aagttttttg aaaatactgt
    19921 ggctgaactg attgctttag aaggagatta ttgtgatatt aaagtaagaa gagctttagc
    19981 aaatagtaag gtattatcag ctgatgtttc tgctggatat gacccaaatt ttggagaagc
    20041 atatgaaaag agaaattcgg catatatggg aaatggagta gttttaacta agtatacagg
    20101 ttcaagagga aagagtggat gtaatgatgc taatgctgaa tttatgtcag aagtaaagag
    20161 aatttttaat aaaggaaatg taatatggca aactgctgaa cttggaaaag tagaccaagg
    20221 aggaggcgga actatagccc atatattggc aaatcaaggt gcagaggtta tagactgtgg
    20281 tgttggagtt ttaaatatgc atgcaccaca tgagattgtc tctaaagtag acatatatga
    20341 aatgtataag ggatataagg cattttttaa tataaattta taagtaaaaa tataaatgta
    20401 ataagtgaat aaaggctgtt gtataaacaa tttaatctgt tgtacaacag tcttattttt
    20461 atgtaaaaat agcaatttgt cacactagag aaaataaaag aaaatactgt caaattaaaa
    20521 attatttaaa aaaaaattga caacgatttc atcttgttat aatatgtaat aaagaacttg
    20581 ttatataatg ttataaaaat gttacatagg gaggtgagca aaatggatga aaagagacaa
    20641 aaaaaagaaa tggatttaga agtaaattcc tttgttcctt tatatcaaca actatatgac
    20701 aacataaaaa aacaaatagc atctggcata tataaaccag gagataaact tccatctgaa
    20761 ggagacttat gtaaagaatt taatataagt cgtataactg tgagaaatgc acttaatgaa
    20821 cttgtaaaag aagatatatt atgtaaaaaa cgtggtaaag ggacttatgt aacaatacca
    20881 gagagaatag aagcaacatg tgcaggaaat agttttacta attcttgtca tcgtattaat
    20941 gcaaaaccaa gtaccaaaat aatctctgtt ttaataaaaa aagcagataa gcaagtagct
    21001 gaagctttaa gtattgaact agaagagaag gtaatatgta ttaaaagact tagattaata
    21061 gatgaagtac ctgttatttt tgaagttgac tattttagaa tagattacat gtttttatta
    21121 aaagaagaat tagaagataa atctttaatg gaggttatat ctaataatat aagcacatta
    21181 cctaaacgag tagaaaatat atttgaagta aaacattcaa acaaagaata ttctgaccat
    21241 cttaaatgtg caagtaatat gccactattg aaagtaagac aatcggtgta tacagaaaat
    21301 aatgatgtct tatattacaa tgagcaattt ataagaagtg ataagtacaa atatgcagta
    21361 tcagcagaaa tataatctat aatttagtag gaggatgagt atgaaaaaga taatattagc
    21421 atctcatgga gacttttcaa aagggctttt aaatgcagta aaaatgatag taggagattt
    21481 agcagattgt gtcagttcgt atggtttgta ccctggacaa agtgcttcag attttgcttt
    21541 agaattagaa aaaacaatat cagaagataa agaatgtgaa tatataattt tatctgactt
    21601 atatggagca agtgtatgta cagcaatgtt aagattaact aatctacata atgttaggtt
    21661 attttctgga atgaatttga atatggtatt agaacttctt acaaggtttt ctgatgaatt
    21721 aacagttgaa gatatagacc aattagtgga agaatctaga agaggaatac aaagcgtgac
    21781 attacaaatt aaagaagaag aagaggtatt ttagtttatg aaaaaaaagg gaatattatc
    21841 agtatttaaa agtaaaaaac caattatagc aatgatacac ttaaaaggag atactccaga
    21901 agatatcttt gaaagagcta aaaaagagat tacaatcttt gaagaaaatg gtgtagatgg
    21961 aataatgtta gaaaattact atggtaatta ttatgattta gaaagaatac ttgaatatgt
    22021 atcaaaagct aatttatcaa taccatatgg agtaaactgt ttaaatgttg atactatggg
    22081 atttgaactg gcaacaaaat ataatgctag ttatatacag gttgattctg tagtaggtca
    22141 tgttaaacct agagatgaag ctactttaga agagtttttt aaattacaac gttcaaaatg
    22201 tccagcgtat ttaattggag gcgtacgttt taaatatcag ccagtattat cagaaaatga
    22261 tgttgaagaa gatttaaaaa taggaatgac aagatgtgat gctatagcag ttacagaaaa
    22321 tgctacaggt caagaaacat caatggaaaa aatagaactg tttcgtaaaa atttaggaga
    22381 cttcccattg gttatagctg ctggagttac attagaaaat gcaaaaaaac agcttaaatt
    22441 aggagatatg gcaataatag gaagttactt taaagataat tacaaagatt ttggggatgt
    22501 tagtgtagaa cacgtaaaaa cattcatgga tgaaatcaaa aaaattaggg aggaattata
    22561 atgattaaat tagtaagagt agaccacaga cttatacatg gacaagtagc atttacatgg
    22621 acaaagtttt taagtacaga ctgtatattg atagcaagtg atgacttatt aaaagatgaa
    22681 ttaaaaatgg caggacttaa aatggctaaa ccatctaatg ttaagttagt aatgaaaagc
    22741 atagcagatt ctataaaagc acttaactca ggtgttactg ataaatacaa cttattgata
    22801 ctttgtgaat ctgtagaaga tgtttacaga cttgctaaag aggtaaaagc tattaaatct
    22861 ataaaccttg gtggaacaaa atcagatgat aatcgtgaaa atatatctaa agcagtgcat
    22921 gtatcaaaag atgatataaa gatgattaaa gagttagatt cagaaggcgt aaatgtattt
    22981 gtacaattag ttcctgatga tgatgctaca aatgtaatga aattaatata attttagggg
    23041 gatgaaaatg gaatttacac aagttatttt gataactcta attgcattct ttgcatatat
    23101 gcatagtttt gtaggttcta caatgcataa tagacctata gttgttgcac cacttgttgg
    23161 tttagcacta ggtaatcttc atacaggtat agtaataggt tcaacattag aattagtatt
    23221 tatgggtgca tttcctgttg gggcaagtaa tcctcctgat tttgtgtctg gttctattat
    23281 tgccacagcg tttgtaatat taacgggtca agatgtatca gcagcagttg tacttgcagt
    23341 tcctatagca actctagtat tgcttataga taacttctta atgacagtag tacttacatg
    23401 gggagctcat attgctgacc gttatgcaga agaggggaat attgaaggcg tagaacgtgt
    23461 gcaattatta tttggtatag gaaataaatt aatattagca ataatagtag gtataggatt
    23521 ttcattaggt gttccagtaa tagaaaaaat actttcattt ataccatcat atgtaactca
    23581 tggaatggat gttgcagcag gcgtgattcc agcaataggt tttgccatgt tagctagaat
    23641 gatgcttaat aagaaaacag tagcattttt attattagga tttatattgg ttgcttacct
    23701 aaacataaca gtaacaggag ttgcattgtt tggattagca atagctttaa tatatgttaa
    23761 ctttgtagga gagaaggagg taatagtaga tgacaacgag ttctaagaaa ttagagacaa
    23821 tttcaccaga tagtaaaata acgaggaaag acttttggaa atgttttaga aggtcattga
    23881 cattagattc atcttggaac tacgaacgta tgcaaaatat agcatatgca tacatgatgg
    23941 caccaataat ccgtagatta tataaagatg ataaagaaaa gaaatctaag gctttaaaaa
    24001 gacatttaga atttatgtca gttactcctc atataagtac tcttttggta ggtatatctg
    24061 gagctatgga agaagaaaat gcaaagaata aagaatttga tgctaatagt ataaatgctg
    24121 ttaaatcaag tttaatgggt ccagtttctg ggataggaga ttcatttttc tggggaacat
    24181 taaagcttat agcagctggg gttggtatag ctttagcttc tcagggtaac ataatgggac
    24241 cgattttatt ccttttaata ataaatgttc ctcattttat tatacgctat atatgtctag
    24301 ataaaggatt taagtatgga actcaattct ttaaagatgt gagtggttca agtatagttt
    24361 caaaagttat ggaagcagct tcaatgttag ggcttatggt aattggaggt atgactgcat
    24421 ctaatgtaat gttaaaactt agtgttaatg taggtagtgg agaatgggca gagcctattc
    24481 aaacttactt agaccaaata atgccatgta tgcttccagc tatgatattc ggtattatgt
    24541 attggttatt aggaaaaaag gtaaagacaa ctacgatatt aatttcagtt atgattattt
    24601 gtatagtatt ggctgctatt ggagtagtat aaataaaaaa atcccaagtt catttgaacg
    24661 agggattttt tgactaaatt aaataaaaaa gaaagatggt tcataataat ttataagcat
    24721 ttatgtgata gttataatta gcttattaat tagttataaa ctcaaaatta agcactactt
    24781 tattaaagtt attatattat aatttcgaaa tactcacata atctgtttag aaatttatct
    24841 tctaatgggg aaaaagtttt ctttttagag taaatgaaga aaaactttct agtaaaatca
    24901 acatccttta ttttataaca ttttatttta cctgcattga gataatcgat agctgaaatg
    24961 tacgatataa aggacactcc aagacctaat ctaaccattt ccttaatagc ctcatttgat
    25021 tctacatgag cgattacatt taatttactt actggaaagt tatttttact aagtgtgttt
    25081 agtattaagt ttcttgtacc tgacccttct tttctcataa taaaatttag ataagccaat
    25141 tctcctatat cgatacaatt atttttatta tcaattttaa aatctgaagg agtgataaga
    25201 acaagttcat catctaataa gtctaggtac tctatttgag gattattgat tttagaacca
    25261 actaatccaa agcttattcg ttcatttaaa atttctgata tagcatattg agaatcataa
    25321 tggcttatgg aaaattttac atctggataa ctcatagaaa aactcttcat aaaatcaggt
    25381 aaaatataag tttcaggtat ggaactacat gctatatcta taataccttc tatttttcct
    25441 gaatattctt ttatatcata aattgcacgt ttgcaattat tcaatatata tatagcatgt
    25501 tcataaagaa tttcaccaga ttttgtgagt gtaataactt tgttgcttct atttataaga
    25561 acagtttcta gctctctctc taaattctgt atgtgtgcgc ttacagtagg ttgagttaaa
    25621 aaaagttctc ttgcagcctt tgaaaaactc tgatgttttg caacagcaac aaatacttca
    25681 agttgtttaa aatccattat ttaatcctcc ttaaattatc taaaactatt ataacatatt
    25741 tgataaatta atagtcaaag gtgattttta aaatagaaaa cctttaaata ttcatggagt
    25801 ttttgattgt ttaggaataa tttggtataa tgttgtaagg tacttacaat aaaaatatcg
    25861 taaattgaat tttcaaaatt caactttaaa gtgtttttta ttacagaaaa attacaatta
    25921 gcttcttaat cttattaaat tcaaataaat attatataat tattttataa tataataaat
    25981 ataattattg aaaaaagaga taactgtaaa ttgagatata gataaatact ataaaattta
    26041 agaaaggagg attgatttat gaagttaaaa aggtcattag tttgtgtaac tattttaggt
    26101 attatacttg ttggatgtca taaagaaaat actaaagaga aaaatcaagt agcaagtaaa
    26161 gcaacacagc aaaaaactat gacaaaagtt caaaatgatg ttaatgaaat tatgaataaa
    26221 gattataagt acattataaa gaatatgggt ataccttata atacatttta ttatataaaa
    26281 cctaaagttt taaaagaatc aaatacaatg caagatataa atacatcaag ttatatgaca
    26341 ttagtttatc caaaatatac tggaaatgat gaactagatg gaagtgcttt atatgttgat
    26401 attaacgaaa ataaagttgt caatgttgaa accaattctt tctcatcaca aggtatatca
    26461 gtcattgatg ctgaatctag tatagtgatt gaaaaaagtg accatgaaaa atcggctgta
    26521 tcactagaaa attttagaca tattgacctg ggagagtatg ttggtgttga agattccaga
    26581 ataaatgaaa ttgtaggaga tgcgaattat gatttaacag catataatca tgaaggcagt
    26641 aaagtagtta agagttatag attgaaagaa gataacaaaa tattaaaaaa agaggtactt
    26701 acaataagta ttgtggataa caaaataaaa tctataaaaa ctattgaaag tgataaaatt
    26761 gtaaaaataa taaaaggaac tttattagaa taaaatattc taaagcatat gatgctgttt
    26821 caagatagaa ggaaatttct agctgggaac agttttttta ttagatttat aaaatttgaa
    26881 ctgcacttat agataactat tagttataat taaatataag ataaaagtat ttccaacttt
    26941 tataatctat aatatattac atagaaatat cttaaataaa aaatatattg gggagtattt
    27001 aaaatgatag aattagaaga tgccataaaa attattgaaa aaaatgtttt accaataaac
    27061 agggtcaaga tagtaaatct tatagatgca gtaaataagg tagttgcaga agatatatat
    27121 tcaacaattg atagtcctcc atttgatagg tcaccctatg atggttatgc atataatgca
    27181 aattctgaaa atagaaattt aaaggttata gggactttgt atgcaggaga tgtatttaat
    27241 agggtattaa ataaaaatga agcagtgaag ataatgactg gtggaaagat accacagggt
    27301 gcaaattgtg taataaaaaa agaagatgta acagttataa gtgatatgat aactttaaat
    27361 ataaaattaa aggaatacga taattacata tttaagggtg aggatataaa aaaggggcaa
    27421 ttgattatta ataaaaatca gaatatagga tatgatggta ttggagtatt agcaagtctt
    27481 ggaatatcca aagtaactgt ttatgatgat ttaaaagttg gaattttgaa tacaggtaca
    27541 gaactacaag atataaatga gactttagag aatggtaaaa tatacgatag taatagatat
    27601 accttatatt caagattatt aaaacttgga atacaagcag atacaatatg caattgttct
    27661 gatgatatat tggagttaga aaaaaaggta aagaatatgt tacagagtgt agatttttta
    27721 attaccacag gtggtgtttc tgttggagat aaagatttaa ttccagatgt attcaaaaaa
    27781 ataggggcaa cagaattatt ttggaagata aacattaaac ctggtggagc ttgttatgta
    27841 ggtacattgg gagaaaagct attatttggt ctttctggta atccacatgc agcaatagtg
    27901 gtatttgata atgtgatagt tcctgttatt gagtatttaa catataagaa gggaacacat
    27961 catataaaag ctatatttaa aggagaattt aataaacttt caaataaaga tagatttgta
    28021 agtggaaaac tatatacaag tgaaggtaaa ttgtatgtag agctaaatga taagaaagat
    28081 gcaaaagcta gattatctgc aactctaaga agtaattgta tgattaagat taaaaagaat
    28141 gagacaataa aagaaaatca attagtggat gttgttatta gatagtttta tgaaaggaca
    28201 caattttatg tataatatat tgtcagttgt ctcatattca ggagtaggta aaactacatt
    28261 gattgaaggc ataataaaag aattaaataa aaaagggtat cgtgttggag ttattaaaca
    28321 tacctgtcat gattttgata tggatgagga aggaaaagat acttataaac atagaaaatc
    28381 tggggcgaga aaagtctgta taatatcaga taaacgagta gcatatattg aagaactaaa
    28441 agagaagaat ccattatata aaatgattca attatatgaa gatatggact tgattatcat
    28501 agaaggatat aaaaattata gatttaaaag attggaagta actagaaaag gtaagtatga
    28561 ggatatttta tctaataagt ctgatttgat aggtattgtc agtgatatag aatataaatt
    28621 gaatattgaa cagtttaatt taaatgatta taaaaatata tcaaagtaca tagagtcttg
    28681 tataaaaaat gaaattttaa gtataagcaa tgttgaaata aaaaacatat taaaagaata
    28741 attttaagat gttcttctta tataaaatga tataattatt tatataaata aagatataag
    28801 aaatttatca attttatttt gagttataaa atattaaaga aattgtagga ggacaatata
    28861 tgaaaaaaat attagtagcc tgtggagcag gaatcgcaac atcaacaata gtttgtgaca
    28921 gagtagaaag attggttaaa gaaaataata tacaagcaga agttgagcaa tgtaaaattg
    28981 ctgaatgttc aacaaaacaa gaaggtgctg atttaatcgt atctactact atactaccaa
    29041 caacttatga tatacctaca ataaaggcaa caggatatat aacaggtata aatacaacag
    29101 cattagataa aaagatatta aatgcattga agtagttttg tatttataaa aaaaatttag
    29161 tattaaatat agagttgtat ctttataaaa ttttaaatat agagatatag ctctttttat
    29221 gttgtttcaa gtatacaact atatgatata aatcttcaat tttatgaaaa agattgttat
    29281 tactttgtaa gtttataaaa gatttaaagt tattgttatg ttatactaat ttatgggtat
    29341 tttttagaag tactaataaa ggagggattt aatacttatg ataaaacaat ttgtcaaata
    29401 ttataagcca tataagaaaa tttttacact agaccttata gcagcttttc tattttcctt
    29461 atgtgattta gtttatccta tgataactag aaatataatg gatgacgttg ttccaaataa
    29521 aaacttaaga atgttagtag tttttgcagt agcacttata cttatattta tagcaaaagc
    29581 agggcttaac tattttatgc agtattgggg acatgttatt ggtgtagata tgcaagctga
    29641 tatgagaaat gaggtattta cacacttaca aagacttcca aatacgtatt ttgataataa
    29701 caaatctgga gtaactatgt ctagaatagt aaatgattta atggatataa cagaacttgc
    29761 acatcatggg cctgaagatt tattcatttc tatagtaatg ttggttggtt cattctttat
    29821 acttatagat ataaatattc ctcttacgct tataatattt gctatattac cgtttataat
    29881 ttggtttgca atagctaaaa aagataagat gaatattgct tttatgaaga gtagagttac
    29941 tataggtgat gtaaatgcta ctttagaaaa tagtatagca ggtatgaagg taactaagtc
    30001 tttttgtact gagaaagaag agttaaataa gtttgtaaga agcaataaat tatttagaag
    30061 agcaagacaa gattcttata aggtaatggc agaatattac tctggtatga atttatacat
    30121 ggatatctta gagtgggtag ttgtaatagc tggtggttat tttacttata taggaaagat
    30181 tactttgggt gattttgctg cttatatact atatgtaaaa atgtttatac aacctatgaa
    30241 aaaattaata aactttacag agcaatatca aaatggtatg acaggtttta aaagattcat
    30301 agagataatg gaacaagacc atcaaaaaga agctaaaaat cctattgaac ttgaaaatgt
    30361 aaaaggtgac attgagatag aaaatatatc tttcacatat gaagataaga ctcaagtttt
    30421 ggataattta agtttatcta taaaagctgg taagactata gcattagttg gaccttctgg
    30481 aggaggaaag actacattat gtaatctatt gccaagattt tatgaatttg ataagggtga
    30541 tataaaaatt gatggaaaga gtataaaaga tgttagttta aaatcattga gaaaaaatat
    30601 aggtatagtt caacaagatg tatttttatt tacaggaact ataagagata atattctttg
    30661 tgggaaccct aatgctacag atgaagaaat gatagctgct gctaaaaaag ctagaataca
    30721 tgactttgta gagactttgc cagatggata tgatacttat ataggagaaa gaggcgtaaa
    30781 attatcaggt gggcaaaaac aaagaatttc aatttcaaga atctttttaa agaatcctcc
    30841 aataataata ttggatgagg ctacatctgc tttagacaat gtaacagaaa gagaaattca
    30901 agagtcttta gaggaattaa gtaaagatag aacaaattta gttgttgcac atagattgac
    30961 tactataaaa aatgctgatg aaatcatagt attgacggat aaaggaattg aagaaagagg
    31021 tactcatgaa gagctagtaa acaaaaatgg agtatatagt aggttacaca ataactagta
    31081 tacaaatttt acaccatagc taacatacac attaaactta caaacatagg atatattaag
    31141 attcataaag acaattcatt ttatatttat aagatgtttt atgaataaaa tgtatcctat
    31201 gttttatttt ttgcaaatta catttaattc tctttgtaag aatagagtta aatagccaat
    31261 ggtcgggata acgtttttac aaaaagaata atttagaaaa ctaactttaa tttatggaat
    31321 ttatcgaatt ttatgaaaaa aatcgaattt tgacttgaaa tgtttttcaa aagtagtaaa
    31381 ataatggata actatacata cattttataa aaatatattt attatgaggg gggattttgt
    31441 atggaggcaa taacagcttt ttgtctagtt ttagtatcac tgacgatagg agatatagtt
    31501 tcagcaaaaa ctaaagcttt tgttccgtcg gtttttgtat cagcaattat atttatagta
    31561 ggattttgga cttttttccc agaaaatatt gtggacttag ccgctttagg aacaccatta
    31621 gctcaattag gtatgctttt gttaataact catatgggaa ctatgatgag tattaaggaa
    31681 ttagcaggtc aatggaaaac aatagtaata gccttggcag gaatagtagg aatatgtgta
    31741 ggtgctttgg cattagggac agtagtattt ggatgggata cagcagtaat agctacacct
    31801 cctcttactg gagggctagt tgcatctata atgatggcag atgctgctac agcaaaaggt
    31861 cttgcaagtt tatcagtact tgcaattttg atgtatgttg cacaaggatt tgcgggttat
    31921 cctattacag cattaatgct taaaaaagaa ggaaaaagac ttttatcaga ctttagaagt
    31981 ggaaaagtaa ctgtaaatac agatgaagaa aaagttaagg atttacctga acaaaaaagc
    32041 agatttaaga taataccaga cttaccagaa aaatatgata cgacatatat gataatactt
    32101 agattaggat ttgtagcatg gttagcagta caatttacaa atcttacaaa tgaagttgta
    32161 tcaagatatg ttatatgttt aatatttggt gtaatagcat cagaacttgg aatagttgat
    32221 agaaaaccac ttaataaatc tggttcattt ggatggttaa tgacatcatt gatggctttt
    32281 atatttgcag ggttagcaaa agctacacca gcaatgcttg ttgaaatggc agttcctctt
    32341 gtaggaatca taatatttgg tgtaataggt atgggaattt tctctattat agtaggtaag
    32401 cttttaggtg aaagtaaaga aatggctttt tcagtttcgc ttacatcact ttatggtttt
    32461 ccaccaaact atatattaac tgaagaggca gtaaaggctt tggctgaaac accagaagaa
    32521 aaagaatttt taatggataa aatgcttcca aagatgcttg ttggaggatt tactacagtt
    32581 acaatagctt ctgtagtaat agcaggtatc tttgtaaatc taatatagta tttaattgat
    32641 aaaaagtatt ttaaataaga agaatgtgta ctacttggag gtatagaatg gatattaaag
    32701 aaataacaaa gagttataaa gactatgtaa ttaagcttag aagggaattt catgaaaatc
    32761 ctgagaaaag tatggaagaa gttagaacat caaagagagt taaagaagaa cttgataaaa
    32821 tagggatacc atatgtatca gctggaggta caggtgtaat tgcaactata aaaggagata
    32881 atccaggaaa gactgtagca cttaggggtg atatggatgc tctacaagtt gttgaatgca
    32941 ctgatgtaga atacaaatct aaaaatgaag gacttatgca tgcttgtggt catgatggtc
    33001 acacatcaat gcttctaggg gcagcaaaag tgttaaatga tattaaggat agtatcaatg
    33061 gaactgtaaa attatttttt cagccaggag aagaagttgg taaaggtgca agagctatga
    33121 ttcaagatgg tgctatggaa ggtgtagata gtgtgtttgg aatacatctt tggactgatg
    33181 tagaatctgg gaccatatcg gtagaagaag gaccaagaat ggcttctgct gatttcttta
    33241 aaattactgt aaaaggtaga ggtggtcatg gttcacttcc tcatcaaggt gttgatgcag
    33301 tgcttgcaag ttcagcaata gttatgaatc tacaatctat ggttagtaga gaagtaagtc
    33361 ctctggaacc attagttgtg agtgttggag ttttaaattc tggaactaga tttaatgtaa
    33421 ttgcgagtga agctatctta gaaggtacaa taaggctatt taatccagag cttagaaagc
    33481 aaattcctgg aattttagaa cgtatagcaa aatctacagc agaagcatat agagcagatg
    33541 ctgaattaga gtatgggtac ttaacaccag cagttataaa tgacaaagaa tgttcaaaga
    33601 tagctacaga tgcagcaatt aagctatttg gagaagactg tataacacta tttgaaaaag
    33661 taacaggagc agaagattta gctgagttta tgaatatagc acctggtgca ctagcatttg
    33721 taggagctag aaatgagtca aaaggtgctt gttatcctca tcatcatgga tgctttaata
    33781 tagatgaaga tgctctagaa attggaactg ctttatatgt gcaatatgca gtggatttct
    33841 taaataaata attttaatat actataaaga aaaagcagag actaagttta tatctctgct
    33901 tttttattgt gtatattttt tataaatatt gactatataa aatatatatg atagcataaa
    33961 gaaaatgata atcaatatca attaaaaaga aggtgattta tttgaataat gtaatatttg
    34021 catttttgat aacattattg gctggtttat ctactggtat aggaagttgt atagcatttt
    34081 ttgctaaaaa aacaaatact aagtttcttt ctataagctt aggattttct gctggtgtta
    34141 tgatttatgt ttctatgata gaaatttttc ctaaagcaca agatgctttg acaaaatcta
    34201 tgggagagaa attaggaagt tggtcaactg taatagcttt ttttataggt atggctatca
    34261 ttgctgttat tgataagcta attccacaag aagaaaatcc acatgaaata aagaaaatgg
    34321 agaatataga agaaaagaat atcaaaaaaa ataagtctct tttaagaaca ggtatattta
    34381 cagcaatggc tatagcaatt cacaattttc ctgaaggatt agctacattt atatcagctc
    34441 ttgatgatgt tacaatagct attccgatag caattgcaat tgcaatacac aatataccag
    34501 aaggtatatc tgtatctgta ccagtttatt atgccacagg agataaaaag aaagcttttt
    34561 attattcctt tttatctggt atgtctgagc ctttaggagc gattattgga tatgtacttc
    34621 ttagaaattt tttaaatgat ataactcttg gaattgtatt tgcaattgta ggaggaatta
    34681 tggtattcat atcactagat gaattattgc catcagctag agagtacggg gaacatcatt
    34741 tatctattta tggattgatt gcaggaatgg gtgttatggc tattagttta ttactattta
    34801 aataactttt aaatatatga ttgtaaccta aaattagtat caaaatgata taattattta
    34861 aatagatttt taagctattt aaaaatatca ttttggagaa tgagtgctat ggaaaagaaa
    34921 ataaaattca aatttgactg gtatagttca cctgataaga tagagaaatg gttagaagat
    34981 attgaagcta aaggatataa tttatataag ataaataaat ttggaggaat attttatttt
    35041 cataagggaa gtccaagaaa aataaaatac actgttgatt actactatga tgtagagaat
    35101 aattattttg atgaacataa atcaaatggc tggaatcttg tatttaggag tattggtagt
    35161 tttatgggta catctgcatg gtatatatgg tcgaaagaat acaaagacat acctcctaaa
    35221 ttatatagtg attctgataa aacaaataaa gtaaagtcaa ttttatttat gaatatgttt
    35281 atattcttta tgttagtagt aatatttata tatttattga atattgaaat aagaaattta
    35341 tatagtggtt atggagcatc tgtaactgct attgtactac agattatatt gttgatttta
    35401 tatgcagtac aattatatag gagattaatg tattatttaa ggcttaaaaa gtttaaataa
    35461 ataaaagaag gtgtcctaaa attatttttt taatatttta agacatcttc ttttgtcagt
    35521 aagtactgtt tttaattata gtaatttctg tctttaaatt gaaatatcaa ataatagttg
    35581 tatgggattg agtattgata aaaataattt aaaaatgtta tcctaaagta tcagatatac
    35641 aaaattatct ttaaatatat tataaatatt ttaattgtat taactagaaa ttatgtaagg
    35701 aggcatttta tgattaagat agcagtttgt gaagatgaaa aagaaacaca acttttaata
    35761 gaagattacc ttgagaatat attaaaagat ataagtatag aatatgagat acaaaagtat
    35821 atatctggag aagagttact ggaaagtaat ttaaaagata tagatatatt acttcttgat
    35881 ataaaaatgg aaaaattaaa tggaatggat actgcaagaa agattagaga agtagacaat
    35941 gaaatggaaa taatatttgt aacttcatta atagattatg tgcaagaagg ttatgaggta
    36001 agagcttata ggtatttact aaagccaata gagttggaag aactaaaaaa acatgtgcta
    36061 acttgtatca aagatattga aataaataag gaaagtcata ttacaataaa aaataagtct
    36121 aatacatata agatttattt aaatgaaata aaatatatag aagttcaaaa aaaagatatg
    36181 ctaatacaca caataaacaa gaattttgat ataaaatata gtttaggcaa aatagaaaaa
    36241 gaattaaatc catataaatt tataagatgc cataaaagtt ttatagtaaa tttaagatat
    36301 gttgaaaata taaagcctaa tactgcaata ctggaaagtg gagaagaggt acctattagt
    36361 agatatagat ataaagaagt taaggagaaa ttcttaaaat ttcttggtga tacaatatgc
    36421 taaactttat gactgagcta ttagtagttt tatctatatt tatacagttt tctataatga
    36481 acggaatttt aatttataaa aaggatagta aaaaaaatga aaaaatagta ttctatattg
    36541 tcttgtttct tatttatatt ataattcaga taagtagatt atttaatgga ggaaattact
    36601 ttagtaatat aaactattta ttttatatat tagcatttat tgcttgggga ggatactata
    36661 atta
//
LOCUS       DAELTA010000020        36494 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.020, whole genome shotgun sequence.
ACCESSION   DAELTA010000020 DAELTA010000000
VERSION     DAELTA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.020"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(300..713)
                     /locus_tag="KPG13_000869"
     CDS             complement(300..713)
                     /locus_tag="KPG13_000869"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG0984612.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(754..1884)
                     /gene="rodA"
                     /locus_tag="KPG13_000870"
     CDS             complement(754..1884)
                     /gene="rodA"
                     /locus_tag="KPG13_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG0984613.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1969..2253)
                     /gene="minE"
                     /locus_tag="KPG13_000871"
     CDS             complement(1969..2253)
                     /gene="minE"
                     /locus_tag="KPG13_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG0984614.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2271..3068)
                     /gene="minD"
                     /locus_tag="KPG13_000872"
     CDS             complement(2271..3068)
                     /gene="minD"
                     /locus_tag="KPG13_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG0984615.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3092..3775)
                     /locus_tag="KPG13_000873"
     CDS             complement(3092..3775)
                     /locus_tag="KPG13_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG0984616.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3873..6851)
                     /locus_tag="KPG13_000874"
     CDS             complement(3873..6851)
                     /locus_tag="KPG13_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG0984617.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMLSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6863..7351)
                     /gene="mreD"
                     /locus_tag="KPG13_000875"
     CDS             complement(6863..7351)
                     /gene="mreD"
                     /locus_tag="KPG13_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG0984618.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7366..8259)
                     /gene="mreC"
                     /locus_tag="KPG13_000876"
     CDS             complement(7366..8259)
                     /gene="mreC"
                     /locus_tag="KPG13_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG0984619.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8269..9279)
                     /locus_tag="KPG13_000877"
     CDS             complement(8269..9279)
                     /locus_tag="KPG13_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0984620.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9400..10059)
                     /gene="radC"
                     /locus_tag="KPG13_000878"
     CDS             complement(9400..10059)
                     /gene="radC"
                     /locus_tag="KPG13_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG0984621.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10104..10688)
                     /gene="maf"
                     /locus_tag="KPG13_000879"
     CDS             complement(10104..10688)
                     /gene="maf"
                     /locus_tag="KPG13_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG0984622.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10908..11879)
                     /locus_tag="KPG13_000880"
     CDS             complement(10908..11879)
                     /locus_tag="KPG13_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG0984623.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11895..12470)
                     /gene="rsxA"
                     /locus_tag="KPG13_000881"
     CDS             complement(11895..12470)
                     /gene="rsxA"
                     /locus_tag="KPG13_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG0984624.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12485..13078)
                     /locus_tag="KPG13_000882"
     CDS             complement(12485..13078)
                     /locus_tag="KPG13_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG0984625.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13091..13660)
                     /locus_tag="KPG13_000883"
     CDS             complement(13091..13660)
                     /locus_tag="KPG13_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG0984626.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13660..14637)
                     /locus_tag="KPG13_000884"
     CDS             complement(13660..14637)
                     /locus_tag="KPG13_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG0984627.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14660..15997)
                     /gene="rsxC"
                     /locus_tag="KPG13_000885"
     CDS             complement(14660..15997)
                     /gene="rsxC"
                     /locus_tag="KPG13_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG0984628.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16213..17157)
                     /locus_tag="KPG13_000886"
     CDS             complement(16213..17157)
                     /locus_tag="KPG13_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984629.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17268..18542)
                     /locus_tag="KPG13_000887"
     CDS             complement(17268..18542)
                     /locus_tag="KPG13_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0984630.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18674..19045)
                     /locus_tag="KPG13_000888"
     CDS             complement(18674..19045)
                     /locus_tag="KPG13_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0984631.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19273..19872)
                     /locus_tag="KPG13_000889"
     CDS             complement(19273..19872)
                     /locus_tag="KPG13_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984632.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(20073..20285)
                     /locus_tag="KPG13_000890"
     CDS             complement(20073..20285)
                     /locus_tag="KPG13_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG0984633.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(20358..22073)
                     /locus_tag="KPG13_000891"
     CDS             complement(20358..22073)
                     /locus_tag="KPG13_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0984634.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(22073..22627)
                     /locus_tag="KPG13_000892"
     CDS             complement(22073..22627)
                     /locus_tag="KPG13_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0984635.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22620..23222)
                     /locus_tag="KPG13_000893"
     CDS             complement(22620..23222)
                     /locus_tag="KPG13_000893"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG0984636.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23231..25879)
                     /gene="polA"
                     /locus_tag="KPG13_000894"
     CDS             complement(23231..25879)
                     /gene="polA"
                     /locus_tag="KPG13_000894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG0984637.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            26031..26118
                     /locus_tag="KPG13_000895"
     tRNA            26031..26118
                     /locus_tag="KPG13_000895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26065..26067,aa:Leu,seq:caa)
     gene            complement(26305..26748)
                     /locus_tag="KPG13_000896"
     CDS             complement(26305..26748)
                     /locus_tag="KPG13_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984638.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(26751..26948)
                     /locus_tag="KPG13_000897"
     CDS             complement(26751..26948)
                     /locus_tag="KPG13_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984639.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(27140..28006)
                     /locus_tag="KPG13_000898"
     CDS             complement(27140..28006)
                     /locus_tag="KPG13_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0984640.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(28232..29101)
                     /locus_tag="KPG13_000899"
     CDS             complement(28232..29101)
                     /locus_tag="KPG13_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0984641.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(29607..29828)
                     /locus_tag="KPG13_000900"
     CDS             complement(29607..29828)
                     /locus_tag="KPG13_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984642.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(30329..31309)
                     /locus_tag="KPG13_000901"
     CDS             complement(30329..31309)
                     /locus_tag="KPG13_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984643.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(31473..32345)
                     /locus_tag="KPG13_000902"
     CDS             complement(31473..32345)
                     /locus_tag="KPG13_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0984644.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(32509..33060)
                     /locus_tag="KPG13_000903"
     CDS             complement(32509..33060)
                     /locus_tag="KPG13_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0984645.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(33080..33982)
                     /locus_tag="KPG13_000904"
     CDS             complement(33080..33982)
                     /locus_tag="KPG13_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG0984646.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(33997..36369)
                     /locus_tag="KPG13_000905"
     CDS             complement(33997..36369)
                     /locus_tag="KPG13_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG0984647.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
BASE COUNT        11216 a         6942 c         3858 g        14478 t
ORIGIN      
        1 aatttttgtt ttattaatgt tgtcataaca acatttactt ggttgaaata gtattaccta
       61 taagtttaaa tatatataat aatagacttg attctatttc atattattaa aatagagtaa
      121 tagtttaaat tctactactc tatctataat ctatttccta attatttaat ctcaaattta
      181 gctcttcatc atttaaatct ttcataagat ttctacaggt actagattta ttccaacaga
      241 aaaactaggg cattaaacac ttatatatct gtttctttag actgtaatat taatattttt
      301 taaatacgtt gacttttgct ttttctaact tctctccatg atagttctcc tctatcaaga
      361 gcttttataa atatttctgc tgaagctaga tttgtagcta ttggaacatg ataaacatca
      421 caaagtctta ttaacgcttg tatatctgtt tcatgagcct gagatgtcaa tgggtctctt
      481 aaaaaaataa ctaaatctat ttcttgtgtt actattaaag aacctatttg ctggtctcca
      541 cctaaaggac cagatgctaa tctatttata tttaattcag tttcatccat tattcttttt
      601 cctgtagttc cagttgcata tagtccatac ttttttaaaa ttgattcata acctatacaa
      661 aatccgacca tagtgttttt catttggtca tgtgctacta atgctatatt catatatcta
      721 atctcctcaa tttatgttta gatgtattta aatttagaaa tttattttct ttcttctcat
      781 acacacattt agtactaatc ctaagtttgc taaagatgtc aataacgaac ttcctccata
      841 acttacaaaa ggtaatgtta ctccagttac tggtattaac gcaaccgtca tacctatatt
      901 ttggattatc tggtaaccaa acattcccat aacaccaacc actatgaggg ttccataaaa
      961 atcttttgca tctcttgcaa ttgctaaaag ccttaatagg aatatcataa ataagattat
     1021 caacactgcc attcctatta caccaagttc ttcacctaca acagcaaata taaaatcact
     1081 atcttgtaca ggtaaaaaat cttcttgatt ttgacttcca ttgtaaagtc ctttaccagt
     1141 tacgcctcca gaaccaattg caatcagaga ttgcataact tgatagtttc cttttagagt
     1201 tacatcttct ggatttagaa aagcttctat tcttaccttt tgatggtctg ccatcattaa
     1261 atacatcagt ggcattgaca ctaatagtat gattattcct ctttttatca actttgaact
     1321 aagacctgct gtgaacagca ttgcaaatat catacaacaa aatacaattc ctgttcccaa
     1381 gtctggttgt gcaatcaata atccgataaa tggaagagaa taaactacaa caggcataac
     1441 ttcctttaac gtatttaatt tatccttttt ggattcaagt atctttgcat aacttagtac
     1501 aaatgttaat ttgactattt cagatgtctg caagtcaagt ggtcctaagt taatccacga
     1561 cctagctcca ccttttactg tcccaattcc aggtaataaa actatggcta acaaaataag
     1621 acttattata taaagcgctt tataatacct gcctagaagg ttatagtcaa aaaataatat
     1681 gactataatc attcctatac caagtacaaa agctaatcct tgtttaatca attgattata
     1741 tgaacctgtt gaattagcat gtgttgcact actaagtata acaagcccaa aaataaagat
     1801 agctaatact gtaactataa gtttccagtc taactgtttt atcaacttaa ttgtcgactt
     1861 ataattaata ttgatttttt tcaaagaatc accgtctttc tatctttaaa catataatta
     1921 aataaaaggg gcatatacca ataaggatat ccccctttta agcactaatt acatacatct
     1981 atcttttata ttctttagtg gtatattagc aactaatgca gatacaggtt tgtcatcatg
     2041 ctcacctctg catttagaca tttttatttc aagaccatca tcttctattt ctgcataatt
     2101 agctattact ttcaaaatgt ctgtttttat catctctaaa agttgtggtg aacaatctac
     2161 tctatcatga actaaaacta gctttaacct ctctttagca acagatttac tagttttagc
     2221 ctcgttagaa aaaactctaa ataaatctaa cacagatgta tcctcctttg ctacttagcc
     2281 atgctaaaca tttttttaag tctaccaaag aatccatctt caacttcaag atcaagtaga
     2341 ggaacttctt catttagtat cctttttgcg atatttttgt atgcttgacc agcaagtgac
     2401 ttagaatcaa gtatagctgg ctctccttta tttgttgata taattatgct ttcatcatca
     2461 ggaacaagac ctaacaaatc tattgctaat atttctatta tatcttgttt atccatcatg
     2521 tctccacgct taaccatatc atttctaatt ctatttataa ctaatcttat ctcttttatt
     2581 tcatttgctt ctaatagacc tattattcta tcagcatctc ttactgctga tatttctgga
     2641 ttagtaacta caatagctct atctgcccca gctactgcat ttttaaagcc ttgctcaata
     2701 cctgctggac aatctattat tatatactcg aaagattctt ttagtttctc acacaggtca
     2761 atcatctgct caactgagac agcattttta tctctagttt gtgctgctgg taataaatat
     2821 agattatcaa atcttttgtc ttttattaga gcttgcttaa gcctacaagt tccttcaact
     2881 acatctacta tgtcataaac tattcgattt tcaagaccca ttactacatc taagtttcta
     2941 agtccaatat ctgcatccac aactactgtt tttttatttt ctaggcttag cgcagttcct
     3001 aagtttgctg cggtagtagt ttttccaacc ccacctttac cagatgttat aactataact
     3061 tcgctcataa tactaccccc tatttttctt tctatttgtc taactttgac aaatagcttt
     3121 cgattacaat tcttccatta cttacaaagg ctatttctgg gctaatttct gattcacttt
     3181 cataattttc ttcatcatct ggagcttctg caatattatc tgctatttgt aatattttag
     3241 gattaagatt tccagcaaca acatatgcaa attcattgcc ttctgcacct gcatgtacaa
     3301 aaccttctat tttacccatt acaaaaacat tacctcctgc tacaacctta gcaccagcat
     3361 ttatatctcc cataacaact gtatttgatt ttgaaaggac ttttgaacca ggtttcatat
     3421 cattcaatat aactacatca ccttcaaatt caatattttc accagatctt agggtattga
     3481 catattttgt tggaaaatta ctattttctt caattttttg atcttcaaca aattccacat
     3541 caaatctaga agtaattcct tctttaagtt ctagaatctc catgtcagtt aaacaatcac
     3601 tattaatttt actaatttta gccccattaa aaaagccaac atatgcttcc aatttgttta
     3661 ttattttttc ctgtatttcc tcaaatggag cttctctttt tatatttact attatccctc
     3721 ttttattacc cttgaactct actagttctt gactacaaat ttctcttaaa gacataacaa
     3781 cctccagagt gatatatcat ttatattctt caaatacgct ttaaaccctt ttattttttc
     3841 tatataaagc taaagtttat acatagattg agctatctat taatttgtga ctcgaaattt
     3901 atattttcgt cagaagaatt gttaacatca ttcgtcttgt tgtcattttt actattagtt
     3961 ggctctaatc ccatataagt tccaataact tctctagttg gtaaaagtga gaatacactt
     4021 gaatcacctt gaggaattac acatacaaca gctatttctg ggtcatctgc tggagcaaaa
     4081 gcaacagtcc acgtaaaaga accataatct tgtttaaatc tatctatttg gtcatctgtt
     4141 atttttggat ttaattcttt tatagctttt cttagatatg aagaattcac cttatcagtg
     4201 tcctctaatc taacttttac tccatcttct aattcttcct ctaatttctt tctttcttcg
     4261 tctttcaaat ctttattttc taatttcttt tttatttcct tttccttagc taatgaaagc
     4321 tctttttctt tttctgcttt catcttatca gctagtttaa tcgcatcatt taggttgaca
     4381 ttataagata acatatgact ttttaaatat tcatactcgt tatctgtagg tattttccca
     4441 cttttctcag ctgtaccagt ttttgctgct acatctattg gaaaatcagc aaaagcactc
     4501 ttcgctgtac cttgtcttgc aaccaatttc ataccttttg ttaattcttt taagttatct
     4561 ggatttttaa aaggtatttt ttcgactttc ttctggtcat taattttaac tgatgaataa
     4621 tcacttgaaa cagctctatc aactacagat aattctacta aattacctcc atttgctata
     4681 gcagctacat atcttgatat ttgagctggt gtataagcat tttcaccttg acctatagct
     4741 aagttaaatg tatctgcagt actccatttt gcaaagttaa aataactaaa tactgctaaa
     4801 tctgctactt cttcaattct atcttcttta acatccaatt tttttagcct attcattgtt
     4861 tcaactctac caggagtttt cttttctgct gcccagctta caatctcatt gattcttttc
     4921 tcatattctt taggattttt ttctctagtt atatcaacaa aatcatttgc catttctctt
     4981 tctaaatttg actttagaag agcttgtgtt gatttcaatt tatcttctgt gctaggaact
     5041 ttacctgctc tttcttctac ttcatcttga agccctgtat tttggtcaag cccaaataat
     5101 tttgcatact caagtatttt atttgcattc atatctatac caatgctttt tccatctggc
     5161 catgttttac ctgtaccaat tgttgccata tatatattac aagactcttg tattgcttta
     5221 tataagtttg tcataccatg gtttcctcta ccatggttcc atacatagtc accaaaagat
     5281 tttttaccta acattataac cccagggtca tttatagtaa agtttggatc cagaccattt
     5341 tctaacgctg ccattgaagt taccatctta aatgaagaac ctggctgaaa ttccccttgt
     5401 gtaaccaagt ttaaaagtgg acttcctgct agtaagtcat ttggattttt tggttgaagt
     5461 gctttataat cctctgtaga aatacctttt gcaaatttat ttgggtcata attaggataa
     5521 cttgatgaag ctaatacttc tccattttta acatcaatag ctataagagc tgctgactgt
     5581 gcttttcctg catatgcact aattggtttg tccccaaatt tacttttaaa agtacctcct
     5641 ttagaagcaa cttcgattat ctgttttaat gcattgtctg aaacttcttg taaatcttta
     5701 tctagtgtaa gatacacagt atctcctgat ttaggttttt tagattcaat ttctttactg
     5761 attctaccca atgcatcaac tttaaccatt ttataaccat cagttcctct taatctactt
     5821 tcattactct tttctactcc tgcaagacct accatatctc cagtttcata acctttttga
     5881 aggtatgatt ctatttgagt ggatggcatt tttcctacat atcctaacat atgagaagct
     5941 aatgacccat taggataata tcttactggt tctactgcta ttgaaacacc aacaaggtcc
     6001 atagcacttt cttcaatttg agcaatcgta gtttctccta catccttagc aatagttact
     6061 ggattatatt gagaatatcc ttttgatttt atcaaatccc ttacgaccat tatttttctt
     6121 gcatcttcat cactcaaact tttatcaatt tctaatgcat cactatttct tactttttca
     6181 aacgcttctt tggcactcag tttagtttct tttatcttat aactagccaa ccaatctctc
     6241 ttatctcttt ctgctgtgaa catccattta ctaactaaaa taggtggata atatccattt
     6301 tcatttagtt ttgcctgtaa tcttgtcgca tccaatcttt tatcttcttg agataatatt
     6361 ccagctgata taagtttgtc caccaagtaa taaaaacttt ccttagcatt ataatcattt
     6421 ggaataccat tttctgattt atattcccta atatctctgt catatgtata atagtacttt
     6481 ccattttcta catatatagg aaactcatct acatattctt caccatttct ctccagcaaa
     6541 tttataagtt ttaatgatat ttcatttgct cttgaatgct tatttgcatc tttcttatta
     6601 atatcgttac ctgaaacttg aacagtaaat aagtttttat ttcctgccaa cagtcttcca
     6661 tatctatctt ttatttctcc tcttggagct tctattgcca actttttata tgttttattc
     6721 tcagctaact cattgtaata ttcatattta aatgtagtca tatagattat ttttactaaa
     6781 atagctaaaa atatcacgcc catgactatc ttaattattt ttagtctatc aacttgttta
     6841 ttttcttcca tgaaatcacc aactaatctt ctttaagttt taaaactgct cttttagata
     6901 agcgatatat tactaaacca attaaactat tcactattgg tgctactagt agacctttta
     6961 caaaaagtgt tgatatgttg tagctttgat aaattatact agatgtacct atattgacaa
     7021 ttgaatcaat taaactgcct ataaaaatta aaacgaaagt aataatataa ctacctttat
     7081 atagctgatc tctcatgtat ccaattgcat atgcaataat aaacaaaatt aatgcattta
     7141 caccaaaaat tccaccaacg gttatgtctt taataagccc tattattgct gctataatcc
     7201 caccttctaa ttcatctaaa taaagagaga ttatggttac atatataaga actaaattaa
     7261 agcttatgcc aaaaatatct atataatttg ttatactatt ttctatagtc ataagcacaa
     7321 tccccaacag acaaagtaaa acttttttca ttgtttttat cccctttatc ctatatttct
     7381 tggttctatg actacaacgt catttatatt cttaaaatca acataaggtt ttacaactac
     7441 atattttaat gacttatctt tatcatcaac cactttttca acttcgccaa ttggtatacc
     7501 atctgggaag aatccaagac ctgatgttgt tattatatct ccttgaatta cattatatga
     7561 tgagtcaaac atatatcctt gcaaatatcc tttactatta taactatctt tattatctaa
     7621 agtagtattt tgagtaattg tacctttagc attggcatcc tttgctagtt taaagcttac
     7681 agatgcctta gaatccaata gtgaaattgc cttactataa ttgttggaaa cttcataaac
     7741 tatgccaact aatccactac cattcataac tatactttct ttttttacac ctgaattttt
     7801 tcctgcacct atgacaaaac tctcatacca gtttccatca tttttaccaa caacacttgc
     7861 tgaaatactt gtcgccttat actcttcttg tacaaagttc agtgtctttt ttaactcttc
     7921 tagcgattct gttttatcta gtttggcatt taaagctatc atattttttt taagtttttc
     7981 attttcttct tctaattttt ttaccttttt agaattatct ttaaaatttg ctacatcttt
     8041 aaaaccattt ttgataaaag aaaatccatc atttattccc ttacctacac ttgtaatagc
     8101 atctgaagct actcctaaat ttactggact accactagaa aatttaccaa ttgatatacc
     8161 cacaattcca attaaagtga tagcaactac gcccgttgct atcactttaa cattaatcct
     8221 ttttttctca tttttgttct tgtcaaatct caaggccatc accaacttct aattcttagt
     8281 attcatcatt aatacttttt caaagatttc ttggtcttca acagatttac ctgttccaag
     8341 tgcaacacaa tcaagaggtg tttctgctat ttgtacagac ataccagttt cctgtgtaat
     8401 aagcttatca agacctctaa gcaaagcgcc tcctcctgtt agcattattc cgttttccat
     8461 tatatctgag gctaattctg gtggagttct ttctagtgta gattttattg catctactat
     8521 tgaacttaca ggttctttta atgcttctct aacttctgta gagcatattt ctattgtttt
     8581 aggtaatcct gagattaaat ctctacctct tatttgcata tttatttctt catcatcttt
     8641 aaatgtagaa cctatgttaa tttttacatt ttcagcagtt ctttcaccta tcataagatt
     8701 atattctttt tttacataag ccactataga ctcgtcaaat tcgtctccac ctattcttat
     8761 agatttagca gtaacgattc ctcctaaaga tattacagct atttctgaag tacctccacc
     8821 aatatcaact accatattac cttctggttc ttcaactttt aatccagccc ctatagccgc
     8881 tgccattggc tcttctataa ggaatgcatc tttagctcct gcttgtcttg cagcctcttc
     8941 tatagctctc ttttctactt ctgtaactcc aaaaggaaca catatagcta ttcttggact
     9001 tacaacacct tttttatcag ccgctttttg tataaaatag ctaatcattg attgagttat
     9061 gtcaaaatca gctattactc catctttcat aggtcttata gctactatat ttcctggtgt
     9121 tctacctatc atttttttgg cttcttcacc tactgctaat acctctttac tatcatctct
     9181 tattgcgaca actgatggtt ctctaacaac aataccttgt ccttttatgt aaactaatgt
     9241 atttgcagtc cctaaatcta ttcccatatc ctttgtcatc ttattaaaac taaaaaaacc
     9301 ttttttttct ttcttttttt ccttagccat gattttactc cttcctacta ttaagaatca
     9361 tgtttaaaac attcttctat tattttatca catataaagt caaattatca tattttcttt
     9421 aaagctaaag tataatccat ctccaataat tatatggtct attatttcta ttccaattat
     9481 ttctccacat ttaataagtc ttgatgtgat atttttatct tctcttgatg gttctacact
     9541 tcctgaaggg tgattatgca tcactattat cttacttgca cttcttctta tagcttcttt
     9601 aaaaacctct cttggatgga ctaatgacga gcttaaagtc cctatagata catctacatc
     9661 agatattatc tcattttttg tgtttaaaag aaccgcttta aaaacctctt tttttaaata
     9721 ccttaggcta tccatataat acctctgtat atcccaagga ttccttattt tatactttat
     9781 aggtttaaaa ctagctactc tagagcctag ttctaaagct gctttaattt gggttgattt
     9841 tgataaccca attccatcta tattacacag ctcctctatt gtcatatctt ctaaatgtct
     9901 aataccctga atatctctat ttattatata gttggctaat tcaatagcat ttttattctt
     9961 gttgccagtt cttaaaagta tagccaacaa ctcagcattt gataagcttt ttacgccttt
    10021 ggcaagcatc ttttctctgg gtctttcctc tggtgccatt tcttttacct taatggttga
    10081 attaaatgac tttttcagat aaatcacact ccataaaaaa gatttatact aaaatatttt
    10141 tttaataggt cgcctaacct tgaaattgga agaccaacta tattaaaata atctcctcta
    10201 atttcttcaa ctaacaaagc tccataacct tgtataccat aggctcctgc tttgtcaaat
    10261 gactcattag ttttcagata atcctcaata tcctgctctg ataacgtttt aaatttcaca
    10321 ttgctgatta catagtcaat taccttttta tcttcgcaca agtttattaa gctaatacct
    10381 gttatgactt gatgctctct tccagatagt ctttttaaca tatctcttgc acaagactca
    10441 tcttttggtt ttcctaaaat agcattgtct aaaacaacaa ctgtatcagc tccaataaca
    10501 acatcttcat tatgttcaga tgctacagac atactttttt caaaagctaa tctcataact
    10561 aaatgttttg gtgtttcacc ttccaatata atctcatcaa tttcattttt aattatatca
    10621 aatcttacat tcgtattctc tagaatttct ttccttcttg gagaggctga tgccaagatt
    10681 atattcattt attcacctca tttgtacgta atttgtacct atttagtcta tcactatatt
    10741 gtaaaaaatt caacagaaat gtcgaacctt ttttgttaca ctattgttat atttattacc
    10801 attagaataa atatacataa aatagaggta tggaaattat tccatacctc tgttttatgt
    10861 actcattaga gatatggaaa ttattccata tccttatttt gtccatatta caaaatttta
    10921 atagctttct ttggacattt ttccatacat aaatggcatc ctacacattt atctgcatct
    10981 accttatgtt tttcttttaa ttctccctct attgcatcaa acttacattg ttttttgcac
    11041 actgtacatc caacacaaag ttcttggtca atagtaactt tctttttctt agttatgtca
    11101 cctgatatta cttttgtagg gcattttgct acacattgca tacaacctac acacttatta
    11161 gggtctatct tagctatttt gtcttcaaat attattgcat caaatttaca agcttttaca
    11221 cacattccac agccaataca tccagcagta catttttctt taacagcttt accaaactct
    11281 ttgctattac actctacaac tacttcttga ctttctggtt ttgtgattat tataccctta
    11341 ggacaaactt ctttacattt tccacaattt acacactttt cttcatctat tactgctatt
    11401 ccatcaacta tagaaatagc atcaaattta caagcatctt tacatgttcc aagacctaaa
    11461 catccaaatt tacacatctt agcacctgag tttaaaacat ttgctgctct acaatctgat
    11521 attccttcat actcatattt atcttttgca cttgaacaag ttcctttaca aataactttt
    11581 gctacttgtt tctcacctgc ttcagcactt attcccatta tttctccaac ttttgcacca
    11641 acatctgctc cacctacagg gcaaccatta actggtgcat tgccttcaac tatcgcattt
    11701 gcaagacctc cacaacctgg aaatccacaa cctccacagt tggctcctgg aagtactcct
    11761 agtatagctt ccactctctc gtctacttct acagcaaact ttttagatgc aaaatctagt
    11821 actatcccga agattaagcc cataattcct aaaactaata cagctgtaag tatcaccatt
    11881 ttcacccctc ttttttatag ctttataagt cctgtaaatc ctaagaaagc tattgacatt
    11941 aaacctgctg atataagcgt tatagggaaa cctttaaatg cttctggtat atcagctaat
    12001 tctagtcttt ctcttatacc tgcgaatatt actattgcta aagtaaatcc agctccggct
    12061 ccaaatccgt ttattattgt ttctactaaa ttataacttt tttgaacatt aactagagct
    12121 ataccaagaa ctgcacagtt tgtagttata agaggtaaat aaactcctaa tgcttgatat
    12181 aaagatggac tcatcttttg aattaccatt tcaacgaact gaactataga agctataacc
    12241 aatataaaag ctattgtttg taagaatcca gttccagttt ttactaataa aatctgtata
    12301 aaataagtta taattgaagc tagagttaaa acgaacgtaa ctgcaactcc catccctact
    12361 gctgtatcaa ccttcttaga aactcccaag aaaggacata tacccaagaa ttgagatgtt
    12421 ataacgttat taacaagaac gatacttaaa aataaaagta ttaaattcat ttattacacc
    12481 tccactatgc ttttttactt cttaatttat taaatcctgc aaataagaat cctaatgtta
    12541 agaatgcacc tggtggtaat ataaatatta atactggttg gaaagatgct ccaaataatg
    12601 tcattccaaa taaactacca tttccaagta attctcttac tgcacctatt attgtaagtg
    12661 caacagtaaa tccaagacct tgacctacac catcaactat tgaaggcata gctcctgttt
    12721 tgaaagcaaa actttctgca cgagctaata ttaaacagtt aacaactatt aatggtatat
    12781 atataccaag agccttatca agagcaggaa catatgcttt taaaagcata cctactattg
    12841 taacaaatgt agctataact actataaata taggtattct tatcttatct ggtgtaattt
    12901 ttcttattaa agatataact aaatttgcac atattaaaac tgctgctgtt gaaagaccca
    12961 taccaattcc atttattgct gaagttgtaa cggctagtgt tggacacata cctataactt
    13021 gaacaaatgt tgggttctca tctattagcc catttttaaa tactttagct aaattcataa
    13081 ttaaccctcc ttatttatta agagcactat tgaaaacatc tatagcagca tttactcctg
    13141 tagtaactgc tgtagatgta atagttgcac ctgatattgc taatatttga ttatctgctg
    13201 ttgtacttcc ttttgctact tcaagaggct cagcagtttt tcctttgtat tgacccttaa
    13261 atgcatcatc cttagctttt gctccaagac ctgctgtttc tgacatatta ccaatgtcaa
    13321 cacctgtaac ttgaccttca gatgatatac ctaccataac ttcaatttct ccaccatatc
    13381 cacttggttt agcttttaaa gtatatccaa ccacttcaga accatctaaa ccttcataca
    13441 cttcagctat aagaccatct ccaaggcctt tgtaagtatc ttccttcatt tctttaaatt
    13501 cttttgcttc tggaaggact gcttttctaa gttcattatt tgcttgtata tttctttgtt
    13561 ctattactgg agctgtaacc tgattagtag ctccaagagc taatgcagct attgcactta
    13621 tagcaagtaa agtaccacct aatctcacca tactgttcat tatttagcca cctccccaaa
    13681 tactctattt ctaacaaact tatctataag tggagcaaga cagttcacta atagtattga
    13741 gtaagataca ccttctggat atccaccata cattcttata acagttgtta taagaccagc
    13801 taatacagcg tatattattt gacctttttt agtcataggc gaagttgtat aatccgtaag
    13861 catgaagaat ccacctaaca taagtcctcc agcaaacagt tgatatattg cgaaagttat
    13921 attaaatcca cctaaaagga atgttaatat aaatacagtt gctatgtaaa atactggtat
    13981 aacataattt attatgcctt tatacattag gtatactcca cctaataata ataatattgc
    14041 tgatgtctca cctatacatc caccaatatt ccctatcatc atgtcagcaa gactaattcc
    14101 attgctagaa aggtctgcca gtcctgctgc tccatttttc aaaaagctta atggagttgc
    14161 tgtagtaact gcatctaaat ttttacctat ccagttaaca cctggttgag tccaagcagt
    14221 cattgctact gggaatgatg ctaataaaaa tgctcttgca gcaagtgctg gattgacaaa
    14281 gttacatcca attccaccaa ataccatttt aactactaat attgcaaata ttcctcctac
    14341 tagacacatc caccaaggaa gtgatgctgg tacgttaaat gctaataata atcctgttac
    14401 aacagctgaa aaatctccta tagtactttt ttgcttcgta aatttttgat ataaaaattc
    14461 agtacctact gtaccaagta tacaaaaaaa catagcactc aaagcattaa gtctaaagaa
    14521 gtatactcct gctactgctg ctggaaggag tgctataata acttgtttca ttatatatga
    14581 agtatcttca ttacttctca catgaggaga agatgatact atcaacttat tttccatcgt
    14641 ttttccccct taactttctt tatttctgag cagcttgctt tcttctcttt gctcctattt
    14701 ctctctttgc tgatcttata gattgaagta atggtcttct tgctggacaa ataaatgagc
    14761 aagacccaca ttctatacaa tccattgctc tatgatattc agctgtgtca tagtcatctt
    14821 ttaaagaata tgcacttatg aaaagtggtt gtaagaatga tggacataca tctgtacatc
    14881 ttccacatct taaacagttt tgaatatctg gagtacgtga ttcttcctca tccaaacata
    14941 aaattcctga agaacctttg ttagtcgcta tttcagtagt atattgagct attcccatca
    15001 taggtccacc cattataacc ttaccaatct tcttatcttc tttaaatcca ccacattgtt
    15061 ctattatttc tgaaactaat gttccaactt ttgttattag gttctttggc tctttgatgc
    15121 aactacctgt tattgtagtg attctttcaa ccaaaggcat acctgtttta atagacttag
    15181 ctatctgtgc tgctgtggca acgttgtcaa caactgcacc tgctgctatt ggtaatccac
    15241 cagatggaac ttctttgcct gtacacgcat aaataagttg tttctctgct ccttgtggat
    15301 atttcacttg aaggccaaca acttcgattt cgctatagtc tttagctaca ttttgaattg
    15361 cttctatagc atctggttta ttcgtttcaa ttcctataaa tccttttttg acatctaata
    15421 ccttcattaa tgctcttaaa ccaaacacaa catcttctgg attttcaacc attaatctgt
    15481 ggtctgcagt taagtatggt tcacattctg caccatttaa tataactact tctgccttgc
    15541 tatctggtgg aggagatact tttacatggt tagggaatgt agctccaccc atacctacta
    15601 ttcctgcctc ttttatgata cctactatat cttctttgct caaatcctct aactgacctt
    15661 ttggttgaac actctcatgt aattcttcct taaagtcatt ttcgataact acacattgag
    15721 ctgaacctcc tggtacttca tgttgttcaa tagcaataac tttacctgat actgaagaat
    15781 gaacgttaca tgaaacaaaa ccttgttgtt cacctatttt ttgcccaaac tttacttcat
    15841 caccaacttc aacaataggt ttagctggtg cccctatatg ttgctgaaga ggaatgtaaa
    15901 ctatctttgg agcttgagct ttttcaagtg cctttgtgtt agagtactct tttctatatg
    15961 gaggatgtat ccctccctta aaagttaaga gtttcattgt tttaccccct tatctataaa
    16021 aaaacatttt ttctgccttc tataagtata ctatatatat taaaaaatct taacaattgt
    16081 taaaaaaata tccgtatttt tataatttat tttataaatc aacacctcta tattaagtca
    16141 aattgaggaa aaggattgct taattaaaca atccttttca tatagctttc taaagtccaa
    16201 attttctgta ttttatttta tagaatttat aaacatagag tactcctgca tggatgataa
    16261 aagcagactt ttacttacac tatatttttc ttcatttgct gcttttgaag ctgtatcaat
    16321 tttttcattc acattcttta catactctat taaattatct ttaaataaac gcatttcaga
    16381 ataatcaatc ttttcagctt cttttgatat attttgtgat attgcctttc tatttgtcaa
    16441 aatagtatta tatttttcca tatctacatt attttcagca ttcgaccaaa aacttgattc
    16501 ttctttaaag tttgttatta atttatttaa ttctttagat atactctcta tataagcgta
    16561 gttacttgta tactctaaag acaacatagg tgcatctaag tgtgataaaa atgcatctgg
    16621 aaacaccttt cttacactgc ttatctgctt tctaacatca ttttcatcaa aagaggaata
    16681 ggtctgtatt ttttttactc catccttctc cataactgaa aaaggtatgt cattattaac
    16741 taatgatgtc tgtatttttt tcaaatcatc attattatct attgcagcaa cttgaatagt
    16801 atacatatcc cagccttcta tagtagctaa tttgacttct tctggctctt tagtgttatt
    16861 taccgctttt tctggattag tttcttgttt atcatctgaa ttggtttgtg atttagattt
    16921 tttccctttt tttattgaat ttaattcatc agatatatct ttataagtta tatctgatga
    16981 attatttaga aatggtattt tagccgatac atactccaat atcttgctat cttttatttt
    17041 tgtataagaa taatagccaa ttagacacaa gcaagcaatc gttataacat atttacgtaa
    17101 atcaatctta tgtctattat tattaaatcc tctgtatatc gtttttcttc tattcattag
    17161 tttgtccctc ccaaaattat attattaata aagtatattt ttaagagtca caatttatga
    17221 atataaataa ttatttcttc taatttttta ataagaaatt gtacttacta aacaaatctt
    17281 cttgcaccaa gtaatacttt agaccaatat gaactagtta aattatccac tttaacctta
    17341 ttaggattag tctgagtacc actacaatgg ataaatttac tatttcctac gtatatacct
    17401 acatgattag ttgttccatc accatcagtg tcaaaataaa ctaaatctcc tggtgcataa
    17461 tttcccatag aaattgcact tccaaaatta gcctgttctc tagaaactct aggaatagaa
    17521 acgcctacag aatgtttaaa aacatactgt gtaaatccag aacagtcaaa tttatcaggt
    17581 cctgaagctc cccattgata tggtattcca ataagagtat atgcaaaatc tacgattgtt
    17641 gaaccttttg ctgtagattt atctgaattt ccagtaaatg atggctttga gttactatta
    17701 gattgagaat tttggttaga tgatgaattt ggtttttctt ttgtatcctc attagtctca
    17761 gaaatataac tggcactcac ccatccaatt gttccactcg ataactttac cttataccaa
    17821 ccattacttt tagcttttaa ttcaacaaca tctccaccat ttaattttcc tattatagaa
    17881 tagcttgtcc caggaccact tcttacattt aaactcacag cagatgttac cttaccattc
    17941 ttgccttcaa ttgattcttc gctagatggt tttttatcag aattattagt actatttgaa
    18001 ttactagaat tgttcgttcc aacatcttct gatgttttag aaatatattg gctactagcc
    18061 catccagtaa ctccatttga tagttttatt ttataccatc cattgctttg ttctagtaac
    18121 tttactacat ctccattatt tgctttacca actaatgaat agttagtacc tgcaccactt
    18181 cttacattca gtctagaact cacatttact ttaccatttc cactaatagt tgtcccactt
    18241 gaagttggat tattttgact tgatgtacct tcagacgaac cacttgttga tatgtatttt
    18301 gcacttcccc atccaacaac agaactcgat acccttactt tataccatcc attagacttt
    18361 tctaaaatct ctacagtttt tcccttgtat aattttgcta ccttatcgca atcagaactt
    18421 ggtccacttc ttatattcaa ggcacttgct gttactgttc ccttttcaag agcacttgca
    18481 tttatggagc taacagatac tgctactgcc ccaattccta aagctgctat agcttttttc
    18541 actactatca ctccccccaa caaattttta tagtcataaa ttcataacat tattatccat
    18601 ttttagtccc tacaatttaa tgataactta ttttcttatt aattgtaaag aaattataaa
    18661 catatcctta aaattagtga tttttaggaa gtatttcaat ccaataatta tctgggtccg
    18721 ctataaaata aattcccata ctttcatttt caaaacatat gcaatctaaa tctttatgaa
    18781 gtttttttgc ctcatcaaac tcctcaactt ctaaagccag atgaaattca ttgtctccta
    18841 aattatatgg tctatcccaa tctctaagcc aagttaattc taatgtatga ctagtaatgc
    18901 catctcctaa ataaactaat ataaaacttc catcttctgc ttccttgcgc tttacctctt
    18961 taagccctaa agctttttta taaaaatcta aacttttttc taaattagta acattgaaat
    19021 tgttatgaca aaaattaaac ttcattttga aatcactctc caatttttat tttttttata
    19081 ttatatcatt attataaatt tatttaaatc atattaagtt aaaaatttga atgatgatga
    19141 ccaattacca aatcatggta aattatttaa aagaatatgt caaaaaaaga ataagctctt
    19201 tcaaatgcat aaaaaattta tacccaagtt atacttaatc atgcaaattg gcagaactta
    19261 ttctttaaaa ctctaacttt taagtttttc tttaataata taatttatta ctttttttct
    19321 aatatcttca gcattataat catcttctac ttctatatcc aaaagttttg ctattgacct
    19381 catatcttct ctagtctttg acctatagct tgatattaat tcatctttta ttttctctgg
    19441 ttctaaaaga tattcttcat ctcctctttt gaaaacaacg tatttttgag catatgaact
    19501 tctattgtag tatatatatc ttgatatttt tcttaaaatt tgattatatg aaagtcgctt
    19561 gttaatatca agttttaagt actctgaaaa agcaaatagc tctttttttg atttaaataa
    19621 tgcttctttc tttatagttc ttgatattga agttacactt tcttgatatg cacacttttt
    19681 taacattttt aaatatactc tattaagacc aatactttga ttgaattttt ttatattatt
    19741 attaataatc ttatccaagt taccttcaaa gtattttttt gctccttttt gtgatacata
    19801 actctcaagt tctttgtggg ccatatcaag tatcattttt tttaaatcct ctttatatcc
    19861 cctagattcc atgatactcc ctcccttagc tatttatagt atattcaaaa agctcaagaa
    19921 atgtgagtca ttacaaaatt tttttacttt ttatgtatat attaatttaa aaggggagat
    19981 aatagtaatg taaaatcaaa catactcaat ctcccccatt tttagacctc tattactccc
    20041 ccaatagagg tcttttttta gtttatcaat ttctattcaa ttccctttag ttcaaatcct
    20101 aattctgcta tcacttcttt taatttttca atagatacat catctttcat atctacagat
    20161 gcacacttat tttctaaatc tacatctaaa acttcaatac catctatatc ttctgttaat
    20221 gcagttttaa gatggtttac acaatgacca caactcatac cttctactaa tagtttcttc
    20281 ttcatttaat tacctcctta taccctataa gggtattcta tcatatagtt tatacttttt
    20341 aaatagaaat taatcattta atttgattca ttcgatttta tatctaaatt agatattccc
    20401 tcatatacat caaaatcatt tggagtaatt ttacctttta atctactatt acttacaata
    20461 tagttgtttc tcacagctaa acttataaat gttgcattat cttttatata tgtctgtaca
    20521 tctttgtatg ctttttttgt ttcaatttca gacgtagaac ttgtaagtgc agacatatat
    20581 ccatttaact tatcatcagt atatccacta ctcgctataa tactacttgc atctggtata
    20641 attgatagtt tccatccaac taatgctaaa tcataatttt tagaattaag agcattttcc
    20701 atatccttat ctgagagctg atttactgta ctatttattc ctatagcttt taagttttca
    20761 tttattatgt ttgcagtttt tattctctca ctattatctc tgtttactat tatctttaag
    20821 ttcatttcag aaagactttt ctttacttcc tctttttctc tatttttttt ctcttcagct
    20881 tcttttttga ttctatcttc ttctgctttt ttaacttttg cttcttcttc tggcgtaagc
    20941 ttctttgagt tctttttagc tgattttttc tgatttttta ctttattgtc attttgattt
    21001 actgagttta catttgcata ttctacctta gcaagacact ctatcgcctt atctttatta
    21061 aactctaaat ccttcatttc actattatag tattttgatt ttgaattaag tggaaaatta
    21121 actggagtag catcatccat ataaccttca ctaataattc tatctttatc tatagagtgt
    21181 gcaattgctt ttctaaaatt aacatctttg aaaaaaggat tattataatt aaataataca
    21241 cactcataat ctcttccttg atattttgtt atattaaact ctttttcttg aaacttactt
    21301 aaatcattta atgtaacatt ggttatatca ctatcaagag ccataaccat agatgtcctt
    21361 gcatcttcat taggcaccat acctactttt atgttcttca tggtttttgg aacttcttca
    21421 tagtaatctt tgtttacaga taaaaccatt ccttctcttt ctgtatatga ttctatttta
    21481 tatttcccat ttcctatcat attattattc ttgtcattta catcttttga atctaaatga
    21541 ttttgagata caattggaaa aattaaacta tcaattgaaa aagcatatct agctttaaat
    21601 tttattgtaa aatctttatc tgatttaata ttcacagaag ctatattttt agtaaattca
    21661 ttatatggac tatctgcact tttttgaatt aaaccaatag tatagtttac atcatttgaa
    21721 gtaacatttt ttccatcatg ccactttgca tcttttagct ttatatctat actcattcca
    21781 tcctgagcta tattatactc cttaactaat tgaggaatta cattgtagtt ctcatctata
    21841 gtaaataaac tatcataaac taaattcatt atataaccaa cagacttatc agtattagtt
    21901 ataggattta tagtttttgg agttaccatt gttaagttta tatacttagc atctatagaa
    21961 ctttgacttt gtaaacttga atcactacta ctttgttttg ttttagtatt actacaacca
    22021 gctaccataa gagttatagc taaaacgact gttaagactt ttattctctt cattagtttt
    22081 ttcctttctt gccatatatc ttattataat gttcgtaatt attttttact ttttctacat
    22141 attttgaagt ttgcttaaaa ggtatatcat gtagattttc gccatcttta ctatattcat
    22201 tattttctaa ccattttttc acattacctg aaccaccatt atatgcagct atcactaaat
    22261 ctaatttacc aaattctttg tataacttac ttaaatacca acagccaagt cttatattag
    22321 tctctgggtc gaaaatatcc acatttttta aatttagttc ctctgctccc caatctctag
    22381 ttatgtctaa tatttgcatt aatccttttg cttccttttt tgaaacagca gaactattaa
    22441 acttactttc ggctttaata acactgtaaa ctatattttc atctaaatta aattctttcg
    22501 aatacttttc tacatactct gaatattttt taggatataa aaatttatgt attactttgc
    22561 tttccattaa tagtgcccca aataagatta taaaaataga tagaatcaat actttcttac
    22621 tattcacgcc aattctcctt catgtactca ataaatttat gtgctttact ttctaattca
    22681 gttattgttc ccgaattatc tattatataa tcaccatatt tggatttttc ttcctgactc
    22741 atctgtgaat ttatcctact aagtgcttcc tgctcactgc aattatctct cttttgtatt
    22801 ctactaattt gtacttcttg tttacaagta actactaata gcttgtcaac catatctaaa
    22861 aaaccacttt ctaccaatat tgccgcatct aacactacta tattttcgcc tttttttctc
    22921 aatttttcta tttcacttat tattttttct cttattcgtg gatgagtcaa gttatttaat
    22981 tccttaagtt tttcttcatc actaaaaact attttcccaa gagcttttct atttagagtt
    23041 ccatcatcat tttttatact ttgcccaaaa tggacaaata ctttttccaa taacaactta
    23101 tcctcaaata tcttcctgga tataatatca gcatccacta taggaatgtt aagatttcta
    23161 aatatatttg ataggctgct ttttccacag cctattcctc ctgtaagtcc taatatcaac
    23221 ataaaatcac ctactttgtc tcataccaag aactacctac atttaaatct acatctaagt
    23281 gaacatccat atttacagca ttttccattt cttcttttac tattttctta actatatcta
    23341 tttcatcatc aattgcttca actatcaact cgtcgtgaac ttgtagtata agttttgatt
    23401 tcaaattgtt ctcttccaat tttttatgaa catttatcat agcaattttt attatatctg
    23461 ctgcacttcc ttgtattggt gcattcattg caaatctctt tcctctattt ctttccataa
    23521 aattgcttga ctttatttct ggtatatatc ttcttctgtt taaaattgtt acagcatatc
    23581 catcctcact agccttttct actgttgtgt ccataaactc ttttatttta tggtatttag
    23641 caaaataact ttcgatatat tctttcgcct ttggaacagg tatatttaaa tcatcagcta
    23701 gaccaaagtc acttttacca tatataatac caaagtttac agctttagct gcacctctca
    23761 attcactagt aacatcttcc ataggaacat taaaaacttg tgaagctgtt ttggtatgaa
    23821 tatcttcatg atgtttaaat gcttctatca tattttcatc ctgactcata tgtgcaagaa
    23881 ctcttagctc tacttgtgag taatcggcat caaccaactt acagttatca tctgcaataa
    23941 acacttttct tatttttcta cccatttcca tttttatagg tatattttga agatttggtt
    24001 ctgtagatga aattcttcct gtagtcgtta tagtttgatt aaatgatgaa tgtattctat
    24061 tactttctgg atttattata tttaaaagtc cttcaacgta agtagaattt aactttacta
    24121 tttgtctata ctcagttatc ttatctatta ttggatgctt atctcttaat ttttctaaaa
    24181 catctgcatt agttgaatat ccagttttag tttttttaat tattggaagc tctaattttt
    24241 caaataatat aacacccaat tgttttggtg aatttatatt gaatggctct cctgataatt
    24301 cataaatttc cttttctaat gtagctatta tttctttaaa ttcaacactc aattcatcca
    24361 atatactttt gtctactttt ataccttcat actccatatg acctaagact tctactaaag
    24421 gcatctctac atgataaaaa agaccatcca tactcatatc cattagactt ctttccatca
    24481 taggaactgt ttctttaact gtgcatatta tttgacccat atactctgct aaatcctcaa
    24541 attctaaatc ctcatagttt ttagatttaa caccctttcc taataattct tcttttgatt
    24601 taaccttttt tgataaatac ttcatagcta ttgcactaca atcatatgag gtagatgaag
    24661 ttgaatctat taagtactca gctattgtta tatcaaaata catattttct aagtttatat
    24721 tatatggttt taaagatata taatcatctt ttaaattata tccatacttt ttaatttcat
    24781 tatttgaaaa tatactatct aactttgaca cttcatttga atctacataa tatatttttt
    24841 ctccatctac acttataaaa atatatatta tatttttttc aagtatatta ccttgtcctc
    24901 taacagtttt taaaataact tgtccttttt tgtttacttc atctataaat ttttctatat
    24961 cttttaattt taatatttct attttttctt ctacgtgttc tgtgtcatct gaatcgacca
    25021 aatcaactaa tcttgataag agacttgtaa atccaaagta tctaaattta tctaaaagtt
    25081 catctttatc ataatcgcca aaaatcatac tatctaaatc cacttctatt ggcacatctc
    25141 ttataattgt tgcaagtctt ttactttgaa tagctatttc tttgttttcc tctattttct
    25201 tttttacact acctttaagc ttatctgtat tctctattag attctctata ctagaaaact
    25261 cttttataag ctttatacct gttttttcac ctattccagg tactccaggt atattatcag
    25321 acttgtctcc cataagaccc tttaaatcga taaattgtgt tggagtcatc tcgtattttt
    25381 caattacaga attaaagttg tattcttcaa cttctccaac tccttttttt gttataagag
    25441 tagttgtatt gtctgaagca agttgaatag catctttgtc tccagttact atgtatacct
    25501 tatatccatt ttcttctgct tttttagaaa cagtacctat aatatcatct gcctcatagc
    25561 catctatttc caatctattt attctaaatt tatctaaaag gtcttttaat ggctgtaatt
    25621 gttctgccag ttcatctggc attccctttc ttccagcctt atactcttta tattctaaat
    25681 gtctaaatgt aggtgctttt ctgtcaaatg ctacacttat atgtgttggt ttatatatat
    25741 ctatcatttt aaatagcatt gttgtaaatc catatattgc attggtcttt agcccttctt
    25801 tattattcat ttctggaaga gcataaaaag ctctatttat tattgaattt ccatctatta
    25861 taattaattt tttttccaaa ttatatcact cctaaaaatt atctattttt gtaacatata
    25921 tattatatac caatatactg ttatttaata ttcccataat aataatagta tattaaaatt
    25981 aaatatgttg caaacaatat atttttttgc tataataata tacatgacat gcccgagtgg
    26041 tggaattggt agacgcagtg gactcaaaat ccaccggact aatactccgt gccggttcga
    26101 gtccggcctc gggcaccatt aaaaactcct aaagctattt attcaataga ttaggagttt
    26161 ttttattttt gatttaaatt ttaagttatt ttttgaaatt actgtgtctt tttaaaattt
    26221 atataattat ataaatttat cttctagcat taatttaaaa actaatttat ttttatataa
    26281 ttttctattt tatatcataa cttattacaa ctttttatct aatttattgt atacaacaac
    26341 actacaaata aattgaaata aaataagcaa tccaagaaca actaaaaccc acttgcttat
    26401 taccattaca gaaaatacac tcaatataga aacaataaat ataagaatta caagaacata
    26461 ggacatgatt tgattagtga tacgtccagc tttatcacgt aacataattc gtctttcatc
    26521 attcatctct atcctttgat tttttatctt ttcttcataa tctactgctc tttcaggttt
    26581 actccaatac atatattgaa gaatcataca tacacctgga cctaaagcag ctccagataa
    26641 tccccaaata aaagcctcaa atgaaaactc agtaaatgta gccaataaaa caagtaaaat
    26701 tccacaaata aaatatataa ttccaacata taacatattc tttttcattt ttactcctcc
    26761 tcataaataa atatttcttc aatactcata tcaaagtatc tagctatctt aaaggctaag
    26821 atgatagaag gattatacct accattttct aatgaaccta tagtttgtct tgatacttgc
    26881 aaaatagaag caagttcttc ttgttttatg cctctttctt ttcttatttc ttctaatcta
    26941 tttttcacat ctatccccct ttaatatctc aactacggaa agtctacttt ccataattat
    27001 acaactatat tccatttatg taaagcttac tttccatatt tacaaaaaaa taacagacta
    27061 ttttcagcct gtaattttag aatttcatgt tgtttattaa ttttaatgtt atttattaat
    27121 tccatattat ttattaattt taacttggtt tctgaattgg aatccaaact tctgtagtat
    27181 aatcaggagc attgatatcg ccattgggat aaagttcaat atctggtaaa ttagcataca
    27241 catacccaga acttggtaac cactcagtaa taattctttt ttgcaattcc tgtattgcca
    27301 gagcatttgg cattggagaa acacattcaa atactgccca tgtgcctgat ggtattaagt
    27361 attcatgagt atcttctaat acaggcttat ctgttggagc agcaatataa taatctaaac
    27421 ccttactatt tgtacacaca ctaactccaa gcaatccaca aggttcatta tttattaact
    27481 ctgaaagttt atcaattgtt ccatttttta tgcatttagc ccacaactta ggaactttct
    27541 caaagcactc ttctacaatc atgttcatac tttcttttat accaacaata cgaaaccctt
    27601 tcttttctac aatacgatat tgcatttcta tatttccttt tacataaatg cttaatgtca
    27661 tcttaggata tgcttttaaa acaacaccct ttgctctagc tgcagatgga gaaatgttat
    27721 gtatattttg aaaagctcta ttaaaagatg ttggtgaatc gtaaccatac ttctcagata
    27781 gttctaaaat tttgatgtta ctgttctgaa gttcaaatgc agccttagtc atacgtcttc
    27841 gtctaatata ttcagataat ggtacttctg caatatatga aaacattctt tgaaaatgaa
    27901 aggttgaaca acatgcaatt tttgctgctt ctgcataatc cactccactt tcgagatttt
    27961 cttcaatata agtaatagct tgatttaatt tatttatcca ttccatttga tattacctcc
    28021 aagcaaatta tacagtttaa taaaataata tacctctcta ttcatgcaca aaattgtaag
    28081 catttaaatt tataagcatt tttcaatcaa tacaataaat ataaaaattg tattatacaa
    28141 taaaaaagtt caataacatt atattataaa atgttattga actttttata aaaattaatt
    28201 taagtgtagt taaattaagt attacactat cttatatatt acaatatcct actatcttat
    28261 agtcttctgg cactttataa tctttgtctg gagtacctaa attccatttt aaatcaaaca
    28321 tagttgcact tattattgtt gcaaaataaa gcataactat accaatgtct attttttctt
    28381 catataaatt agtatgacca tcttttctaa cagctaaaac tacagtgcct ccatcaacta
    28441 taaatctcca tggttgtcta tttaatgtag atggagctaa tcttgcatag tgaaatgcat
    28501 caagaagtgc tctttcttct aagatttcta tacttgcaac atttccccat tcgttcatat
    28561 aaacaacatc ttctacacct agtctagtag accccttatc tgttacaact tgcatatcag
    28621 attttgaata attttgccca gtttttgtag catttaatac tttagcatta cttacatttt
    28681 ctccatatcc aatagctata attcctgtta cctctttatc tgaattaatt ccaagttttt
    28741 cttttacctt ttttccatct tcaaaagtaa cccaacaaga accaattcca agttctgtta
    28801 ctttaaagat tatactttct cctatgtacc ctgaattttc aatgtaatta tcttttatgt
    28861 cagataatat tataatataa cttggagaat ctatcatatt tcctttgtac cctgctactt
    28921 tatctaaatt attaaaaact tcacttctat tcataattct tacatcaaca tctatttctg
    28981 gaactagttt cttacaagat tctgagtatt tcttaatttc actaagatat tcttctttaa
    29041 tatctgtgtt cttatattct ctaacagact ttctatttaa tatgattgtt ttataatcca
    29101 taattttatc ctccccaaac tagtattgtc ttaattatat acgcattaat atattatttt
    29161 actcatttat ttaataaata aagattacaa ttttgtaatt attatttatt aataatattc
    29221 cacttttaca ttaataatga ccagttgtgc aaaatccatt gccaaccacc aaataaaaac
    29281 atattataaa ggtaatataa aataacaaac aaatcttaac aaaaaattaa acttttttta
    29341 cacgaaagaa ggcttcccgt tgccttcttt atttttataa aaaaataagc tgtttctaaa
    29401 atgatttcac atttttgaaa cagccaagtt attctataat gacaactata atttgaggat
    29461 gtgagggaga gttgtcatta taaatttagg gtaattatat tatgttttaa gctagttctt
    29521 tctatattta tataatacat tttatatatg aattttatat gagattggta taaatgatat
    29581 atgaaattac atatgtttat attatattaa attttattat tacattttcc acaacaatac
    29641 tcatatgtct ccacattatt tggaatattt acaaatacaa attctttaca gttctcacaa
    29701 tactttaata tagatatagt tccacaagtg taacattcaa atattacaca atcctcattt
    29761 tcctctacta tttcaaactt tttactatgt tcaccacact ctgggcactt gtgattattt
    29821 acattcataa atactcctcc ttattagtat agttacatgt caaaaactta attaatgtat
    29881 tctatattac ttattatata ttctataata aatcatattc taatataata aaacaaatta
    29941 aataaaaaaa aaccataaat tttaaattta tggattaatt cttgtaatga taactctatt
    30001 tgaggatgtg agggagggtt atcattacaa tagggtatta tattatggtt tttaagctag
    30061 tctttctaca cctcatatga tacatatgct atatgaattt tatatgattt ttacataaat
    30121 attttatgaa atttaaattt tttagtcatt ttgaaatttg taaaaagttc tctaaaaatc
    30181 cacagcatca taaacagcca aaatttaagt agaacatcat aaaaaaattt gtccaataat
    30241 tttaacaagt aatctcacct cattataatt attgacacaa acttaaataa acaatctata
    30301 aatcttctat aaatcttaaa ctttccccct attgatacac aatttctagt ttgtcaattt
    30361 tattttgcat ttctaagaaa atttcttcaa cagaattgtt aaatatattg cctaaataga
    30421 ttcttttatc tttttgaaat gaataagatt ttacactacc atcagaagaa atatataaat
    30481 gactccactc tgctccatat tttgaaaacc tctcctttgt atctttaaac ctcatatctt
    30541 ctatttttat tgctttttct ctgtctcctc ttactacagt gtgacccctt tttattaaac
    30601 tggttatttt tatatcttct tttaatttaa tacgtgtatc atcatttttt gaatatactt
    30661 tccatatcac tttatgacga taattatcta attcctttat taagcttcta tcaatagtaa
    30721 aactatcttt agttaccaca attaaatcaa aatggtctaa catatattga aatatttctc
    30781 tcatactttt attttttaaa atatcctcac caataacctc tataaatcta cagcctttct
    30841 catacatgca gttcaataaa cttgtgattt cagaaaaata atcatttgta ttattatctc
    30901 catctatttc tatagtaaag tacaaaggag taatgtatga tttatttcct gttatctcaa
    30961 atacacttga tagtgtctgt atatcatctt caaacgttaa gtatacattc tcataaaaca
    31021 taaaaaattc cataactttt tctttcatat tatggatatt tctatatcta ttggataata
    31081 tatttattat atcgtcagta gtatttttcc catcacattc agccagtata acaatttgct
    31141 tccaacttaa gctatccaca tttccgatac tttttttata tccatcttct tcatcataaa
    31201 taggaccaat tataatgttt tcttttggcc acagatgaac cctagttcca tcttttaatt
    31261 tcggatattt attttttaca gactcataaa atctacttgt atcacccatg attctttact
    31321 accccccata gtttaaatat tctatgctaa aaatagcact ttatttttat taattcaaca
    31381 attattcaaa attggtcatt atatatgttt aaatggattt aacatcaacc ttatttatat
    31441 gttattgatg tttatatatg ttatataaaa tcttatttct catacttcaa tgtaatctta
    31501 actatctctg gtttacaatt aagcctaatt aaaacttttg agcctcctaa gcctttattt
    31561 aaaatcatag tgctattatc tttttcatat tttccccagt catacttagg aaagaactct
    31621 ctatttggag ataatacacc tccaacaaaa ggtattctta ttatacctcc atgaacatgt
    31681 cctgctaaaa ccaaatctgc tccccatttt gcatattttt caaagaaaaa tggtgtatgt
    31741 gctaaaagaa tgttatattc attttcatta actttaccta gattgtcttc tacaaaattg
    31801 tcacttagtt ctaatttttt gctattagca aataaatatg gatagcattc caatggtata
    31861 attaaaccat ataaattaag atggctatta cctctatata ttcttacaga ttcattttcc
    31921 atatttatta ttttctttga atatagttcc ttaaaataat ccttatatag atgtttatac
    31981 ttttttatta aagatttttg ttcatgattt ccaattatat gatatacttc atatttttca
    32041 accaaattat ctattaaatc taatgcaact tgaaaatttt tattttctcc atctactaaa
    32101 tctcctgtta taaatataac atctgggttt aatgattcaa ttttatctat caatacctta
    32161 ttatttttat caaacacctt attatgtaaa tcactcactt gaactatttt aaagttatca
    32221 aattcctttg gaattttttt gttgaaaata gtatattctg tcgtttctat atgctgagtt
    32281 tcatacttat acagaatata caataatatt aatactaata ctgtcattga aacctttata
    32341 atcatattct cttcctcttt attctatttt gttaatatta ttataatgct tttatgtttt
    32401 ttttaaaaca ataacatcat cacatacata taaatctttt cgacattata aaaataagtt
    32461 atccttttct aaaattttca tttattttag aaaaggataa ctataaaatt atctataaac
    32521 ctctatattg tgaaatatag tttttccttt atttaaattt ttatatatat gctctaaagt
    32581 tccatcaaac ctaatagaat gtccagactc taaaatataa gttttcccat ccacattaag
    32641 gtcaagttta ccttctatta cgagtacata ctcttctaaa acatgactgt gtgtagatga
    32701 aacatgagta cagttttcct caagctcaat tataaaaagt tcaaaccttc tttgagggct
    32761 aaaagggtat attggatata acttcatgtt ccctttttgc tcaacaatag gctctacttc
    32821 ttctttcttt ataacctcat aggtatttgt attttcattt aatagtgtag taaaatttat
    32881 tttaaatcca gatgaaattt tccataaagt agatactgtc ggacttgatt cacctctctc
    32941 aatctgtcca agcattgctt tactgacacc tgtcatcttt gaagctttgt ctaaacttat
    33001 gcccatttct tgtctcaata accttaaatt ttctcccaca ttaggaaatt tattatccat
    33061 gttaaaatcc tcctgatagc tatttgtatt ctatgatttt tactcctgta agttctacta
    33121 tctcttttaa ttttaacata tgattgttac aaatttcatc aaattcatat atataatctt
    33181 caagccctaa tgccttatat ttatcgattc caaatttatg atatggaagt aactccatct
    33241 tgcctcctgg aacattttgt ttgacaaata atgcagtatt tcttatattt tcttcactat
    33301 catttactcc ataaataatt ggcactctta taaccataga tttattcaac tttgaaagcc
    33361 tacatatatt atctaatata attttattac ttactcctgt atattcttta tgaatattat
    33421 catccataga ttttatatct acaaatatgt ggtctatttt ctcaaataca tcatttacct
    33481 tattccaatc aaaatatcca caagtttcaa ttgcaatatt tatgccttta tcataaaaaa
    33541 tatcaactaa ttctcttaaa aaatcaatct gaagtgtagg ttcacctcca gaaaaagtta
    33601 tccctccatt agattcaaag aaaaatataa aatctttttc aacttcctct actatttctt
    33661 caacactcat ttcttcggtc attatacaaa tggcatcttt aagacatacc ttgatacact
    33721 ctccacataa atcacattta ttatttattt gcgctctatc aaatagtgaa cttatatttt
    33781 gaggacaaac atctatacat ctattacagg atacacattt atctttcata acacctaatt
    33841 ttactatgtt actccaggaa tctggattag aacaccactt acattttaat ttacatcctt
    33901 ctaaaaatat agtagtcctt attccatctc catcatttac tgaaaaatgt tgtactttta
    33961 caactctacc ttttttactc ataatctacc tcataattaa aaattgctat gtgaatttct
    34021 agctattata gcatcttgca tttctcttga taaattaaca aattgagtac tatatccagc
    34081 aactctcact aataaatctt tatattcttc tggattttct tgagctttta atagagtatc
    34141 atttgatatc gtattaaatt gtacatggaa tcctcctaaa gcaaaatatg atttaatcat
    34201 agatgttaga tttctaagtc ctctttcagt ttccaccaaa tcttgattca gtcttaaatt
    34261 tagaagtgtt ccaccagtat gaacatcatg atttattttt gatgcagatt tcattgctgc
    34321 tagtggactt gttgtatcag taccaacaaa tggagaaact ccctctgtca aaggttttgt
    34381 cgcttttctt ccacaaggag ttgcgcctac gatttttcca gttggtacat agtttgatat
    34441 acccataaat gcactattaa atttagagcc aaaaatatcc ttgtattttc tagtttcagt
    34501 ataataaaaa tcagaaactt caattgctaa tgaatctaca taatcattat catttccata
    34561 cttaggaaca tccaacgcaa gttttcttaa ttcttcataa ccttcccaat cattatttag
    34621 tgcttttgtt aattcttcta acgtagtcac cttatcctca aatactaatt tttttatagc
    34681 tgccaatgaa tttgaaacta cacctaaccc tataccagtt aatacaggac ctatattata
    34741 ttttgctcct cctttactta aatcaactcc cttatccaaa catccatcta cacaagtaga
    34801 taaaaatggt cttggtacca tttctttatg aatttgttga gcaacagtag ttcctataac
    34861 agaacttttt acaagatatg ctaattgctt taaaaactca tccttaagtt cttcaaaaga
    34921 agtaaattct gtgatatttt ttgtatctaa ccccattttt tctccagtta atctactctt
    34981 accttcattc atagcatatt ctaaagctgc tgcaaaattg atatttacgg ctgaagtcca
    35041 ctgtcctgtt tttctaaaat gaggaactac acaaccacag ttactccaat ctctagcatc
    35101 ttctgcatca tatccatctt gtaataacat ttgtgcccct gcactatcgc tgtgtatagc
    35161 agggaaacca gttccttgtt ttaccaattt agcaactgcc attacgaaat tgtctggtgc
    35221 tccttgactc actctaacac ttaatcctgg ttggtgagtt tttacacttt ctgtagcctt
    35281 taaacacata tatgagatat cattcgttcc atcagtacca tttctctttt taccaccaac
    35341 agtaagattt tggaattgat tatatcctgc aaagtaattt gctgtatttg ctgatatagt
    35401 ccatacccat tctgataact taatccaaag tgcttctatc aattcttgag cttctgattc
    35461 agttatttta ccttctctag catcattttc ataatatgga tacatatatt gatcaaatct
    35521 acctaaattt aatgccaatg gattttctga tagaatacca cctaattgaa caaaccatac
    35581 aaattgtatt gcttcataaa aattagttgg agggtttgct ggtacattca tacatatctc
    35641 tgaaattttt ataagttcta actttctctt ttcatctgtt tcttgcttac tcatctcttt
    35701 tgcaagattt gaatatcttt gtgccaatgt cataataccc tgtgcaacta tagaaacaga
    35761 tttgtaaaaa tctatctttt caatattttc tgaaactgat atatctaatt ctttaatttt
    35821 tttatctgca tcttccttaa tccctttata tccttttaca aacaatatat cttgataatc
    35881 tggtgttact tcaccaactg cctgtctcca cttagaatca ttatctatta ttcctgtatc
    35941 tactaatatc ttagctgtat cttctgggat tcttgctaag aaaacttctt ccaaagattt
    36001 tcccttccaa tatggaaata tttcttttct tataaattct atttgttctt ttgatattac
    36061 atatgggtct tgagttcttt tatcaaaagt atccatttct ttgtctaccc attgccaaga
    36121 aaactctggt gtcaatatcc ctgttcttct aaattttcct gctgtaccta ctattaattc
    36181 attttcaaag atttttatag gaagttcttt acatgtctcc aaaaaagctt tagctcttct
    36241 tatacagata gcttctccct cagttttttt atgtgattct gtaaaaattc tagccctttc
    36301 acaacatatc ataggtttag aattaatata ttcttgtcta aatgcttcta ttctattagt
    36361 ttgactcatt taaaatcccc ctttggataa tatatactac atttttgtat gttataacat
    36421 actttaatta tattatactg tcctttagca taaaatggaa gcaaaataac ttattttttt
    36481 tcatctacct tgaa
//
LOCUS       DAELTA010000021        35747 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.021, whole genome shotgun sequence.
ACCESSION   DAELTA010000021 DAELTA010000000
VERSION     DAELTA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.021"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..895
                     /locus_tag="KPG13_000906"
     CDS             <1..895
                     /locus_tag="KPG13_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0984648.1"
                     /translation="NNVSTISNNIILYTWIITVVVYLIYTIFKYIKLKNLINDLSYDV
                     DDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRH
                     ELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKK
                     DYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSI
                     TFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEFTYAEAPKSMREEHKINCEFLGL
                     IPKDSDKIGVGIEYYDSLLND"
     gene            1811..2833
                     /locus_tag="KPG13_000907"
     CDS             1811..2833
                     /locus_tag="KPG13_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0984649.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3129..4049
                     /gene="pyrB"
                     /locus_tag="KPG13_000908"
     CDS             3129..4049
                     /gene="pyrB"
                     /locus_tag="KPG13_000908"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG0984650.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4088..4780
                     /locus_tag="KPG13_000909"
     CDS             4088..4780
                     /locus_tag="KPG13_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG0984651.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            4773..5675
                     /locus_tag="KPG13_000910"
     CDS             4773..5675
                     /locus_tag="KPG13_000910"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0984652.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            5760..6344
                     /locus_tag="KPG13_000911"
     CDS             5760..6344
                     /locus_tag="KPG13_000911"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG0984653.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6558..7484
                     /locus_tag="KPG13_000912"
     CDS             6558..7484
                     /locus_tag="KPG13_000912"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBG0984654.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7536..8426)
                     /locus_tag="KPG13_000913"
     CDS             complement(7536..8426)
                     /locus_tag="KPG13_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0984655.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8614..9645
                     /locus_tag="KPG13_000914"
     CDS             8614..9645
                     /locus_tag="KPG13_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG0984656.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            9939..10814
                     /locus_tag="KPG13_000915"
     CDS             9939..10814
                     /locus_tag="KPG13_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG0984657.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            10832..12322
                     /gene="cls"
                     /locus_tag="KPG13_000916"
     CDS             10832..12322
                     /gene="cls"
                     /locus_tag="KPG13_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0984658.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12515..12799
                     /locus_tag="KPG13_000917"
     CDS             12515..12799
                     /locus_tag="KPG13_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG0984659.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            12832..14460
                     /gene="groL"
                     /locus_tag="KPG13_000918"
     CDS             12832..14460
                     /gene="groL"
                     /locus_tag="KPG13_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG0984660.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            14722..15462
                     /gene="larB"
                     /locus_tag="KPG13_000919"
     CDS             14722..15462
                     /gene="larB"
                     /locus_tag="KPG13_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG0984661.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15546..16280
                     /gene="larC"
                     /locus_tag="KPG13_000920"
     CDS             15546..16280
                     /gene="larC"
                     /locus_tag="KPG13_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG0984662.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            16310..16729
                     /locus_tag="KPG13_000921"
     CDS             16310..16729
                     /locus_tag="KPG13_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG0984663.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCSES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      16789..16890
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17111..18646
                     /gene="guaA"
                     /locus_tag="KPG13_000922"
     CDS             17111..18646
                     /gene="guaA"
                     /locus_tag="KPG13_000922"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG0984664.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            19185..20615
                     /locus_tag="KPG13_000923"
     CDS             19185..20615
                     /locus_tag="KPG13_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0984665.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFLLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(20763..21113)
                     /locus_tag="KPG13_000924"
                     /pseudo
     CDS             complement(20763..21113)
                     /locus_tag="KPG13_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            21108..21629
                     /locus_tag="KPG13_000925"
     CDS             21108..21629
                     /locus_tag="KPG13_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG0984666.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(21758..21994)
                     /locus_tag="KPG13_000926"
     CDS             complement(21758..21994)
                     /locus_tag="KPG13_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984667.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(22304..25732)
                     /gene="uvrA"
                     /locus_tag="KPG13_000927"
     CDS             complement(22304..25732)
                     /gene="uvrA"
                     /locus_tag="KPG13_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0984668.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            26051..28285
                     /locus_tag="KPG13_000928"
     CDS             26051..28285
                     /locus_tag="KPG13_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0984669.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28541..30571
                     /locus_tag="KPG13_000929"
     CDS             28541..30571
                     /locus_tag="KPG13_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0984670.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30568..31020
                     /locus_tag="KPG13_000930"
     CDS             30568..31020
                     /locus_tag="KPG13_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0984671.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            31022..32077
                     /locus_tag="KPG13_000931"
     CDS             31022..32077
                     /locus_tag="KPG13_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG0984672.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32144..32452
                     /locus_tag="KPG13_000932"
     CDS             32144..32452
                     /locus_tag="KPG13_000932"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG0984673.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32556..33821
                     /locus_tag="KPG13_000933"
     CDS             32556..33821
                     /locus_tag="KPG13_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG0984674.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            33909..34625
                     /locus_tag="KPG13_000934"
     CDS             33909..34625
                     /locus_tag="KPG13_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG0984675.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(34820..35113)
                     /locus_tag="KPG13_000935"
     CDS             complement(34820..35113)
                     /locus_tag="KPG13_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG0984676.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(35119..35334)
                     /locus_tag="KPG13_000936"
     CDS             complement(35119..35334)
                     /locus_tag="KPG13_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984677.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT        13727 a         3874 c         6227 g        11919 t
ORIGIN      
        1 taataatgtt tcaactataa gtaataatat tatattgtac acttggatta taactgtagt
       61 agtttattta atttacacta tatttaaata tataaaactt aaaaatttaa taaatgactt
      121 atcttatgat gttgatgatg gagagattgt aaatctttat aaaaatatat tagaagaatt
      181 taatataaca aaagatataa aactcaaata ctcttatgaa gttgaaacac cagcattttt
      241 taattcatgt gtgcttttgc caccacatga gtataaattg aaagagttag actggatttt
      301 tagacatgag cttatgcatt ttaagagtag ggacctttat ttaaaatatg taatcctgtt
      361 tttaaaaaca gtatattggt ttaatccatt tatatatata atggataaac atatagattt
      421 agattgtgaa ctttattgtg atgaaagagt tttaaaaaat cgaaattcag aggaaaaaaa
      481 ggattatgct ttaacaattc taaatgcaat gagaaaaggt tcaaatactt caaataaatt
      541 tatagctgga ctccataagc aatctgatat taaaaagaga gttatgaata tgtttaatga
      601 aaagtacaag aatggtatat taatggcatt aactttatgt ttattatctt ctattacttt
      661 tttgaaggtt aattcaacga gtattgttaa tcttaatata ttgccaaaag gaatggaaga
      721 aaaagatgtt actaatagtg caattgttac aatgatagaa tttacatatg cagaagctcc
      781 taaaagtatg agagaagaac ataaaataaa ttgtgaattc ttaggtttaa tacctaaaga
      841 ctctgataaa ataggtgttg gtatagaata ttatgacagt ctattgaatg attaaataaa
      901 tattatttta acagatatta attataaagt tttgaatagt aatttgttga gaaataaata
      961 tatccttata aaaatagata cttatatacc aagaataaaa aatagtcaag atagtacaca
     1021 aggatatact tataaagaac tcaaataagt gaaataaatt attaattata aggtttataa
     1081 ttttaaaaaa ttagataaat tcaggtgtaa aatactataa aacacaattc ggaaaagtgt
     1141 atgttgaact tcatttaaaa tggaatttca aagttgctat agtaagtagt ttaagcattc
     1201 ttcttatatt aatctttgag aataatgtat ttaaaaaagt tagtaaaaaa tttaactatt
     1261 gtatattaat gatagttgct attgatatat tatctaaaaa aattaagtat gaaaagatta
     1321 ctgctaggac aatcttttca tacttaattt ttaatattta tattatccaa aattttcatc
     1381 tttttaactt agaatttgag aatcactatt aggaaatagg ctttgattta tgtagggtta
     1441 aaaatttcac agaatatcta actttatact aaatccatca aaaaatatac gccatataaa
     1501 agcgcataaa aaaacacata aaacaatatt tatgcgcact ttaacctcga atggtacaaa
     1561 attctttact tttacagacc catattatat aattaagtaa ataatgtata tttaggtgac
     1621 atattggata agctaattta tttattttat taatataatg atactataaa agttatataa
     1681 agtggtttac aaaaaagtta cagatagagc ttttattaat gtattgtgta ttagtatttt
     1741 atccttaggt acacttcacc tacaaaatag aatcataaga taattatggg aaaattttag
     1801 gaggtttatc gtgaagaaaa agatattaat accagtattt gcatcagtta tggcattatc
     1861 agtaagttca attgtaaatg cagatgaagt aaatgattca tctcaaaata aggatgataa
     1921 aacaaatact gaattaaatt taggtgaata taaagaagta aaatataaag ttgcaaaaat
     1981 aaaagatgga gtagctataa agataagaga agaaggtcaa gttcaaaaca tagcttattc
     2041 tggagatgaa tttacggttt taggaactca aggagaatgg gttaaggtta aagttgaaga
     2101 tggcgaaggt tggttagcta ctagatatgt agatatatct gaaggtgtag gatatactaa
     2161 cgctgataaa gtaaacttaa gaaaagataa gagtgaaagt tcagaagtaa ttgaagagct
     2221 ggaaaaagga agttctttat tggttttgga agataatgga gattggttaa aagttaaaga
     2281 tggggaaaca gaaggatatg taaaatcatc ttatatatct gataaagctc ctgtaataga
     2341 agagccacaa gtaaacccaa gtattgacca aaatgtagat tctacaaata ataatcaaca
     2401 aaacaatgca aatactaatc aacaaaataa taattcaaat gtgccaacag ctaactctaa
     2461 tgcagttcaa gctgtactta acttggcata ttctaagcaa ggttgtccat atgtttgggg
     2521 agcagaagga ccaaacactt ttgactgttc tggatttaca caatatgtgt atagaaatgc
     2581 agtaggaaag aatataccaa gaacatctaa agcacaatct aaatatggac aaactgtaag
     2641 taaagcaaat ttacaaccag gagacttagt attcttcaca actaatggtt ctggttctgt
     2701 tagtcatgtt ggaatatatg taggtggggg taatatgata cattcaccat caacaggaaa
     2761 aacagttagt gtaactagta taaattcatc atactataca gcaagatttg ttacagcaaa
     2821 aagaatatta taatcggttt gtaatatgac aaattgataa atacataaat atatataaac
     2881 gctctatatt atatttaatt taatcatgga aacaaaaaat gattatattt taaatgtact
     2941 atagagcgtt tttgtatcac tttaaatcta atcaagattc aagttaatat gactgttaaa
     3001 taggagcctt ttcatatgat gattatttgc gcaatccctt gacaggatag ggcaaaggtg
     3061 gtaagctact aacagtacaa aaatactttt aattttagtt cagagaggct aaaaaaggag
     3121 gacgaaagat gttaaaatca agaaatttaa tccagccaga agacttttca atagaagaaa
     3181 tagatgaaat attagaatta gctcaaaaaa taattgacaa tccgtcaaaa tattctcgta
     3241 tatgtgaagg aaaactatta gctacattat tttatgaacc atcaacaaga acaagattaa
     3301 gctttgaatc tgctatgaac agattaggag gacgtgtagt aggcttttca gagccaaatt
     3361 catcctctgc atcaaaaggt gaaactctag gagatacaat gaggatagta tctggttatg
     3421 ttgatataat agctatgaga catcctcaat caggtgcagc ttcagaagca gctagatata
     3481 cggaagtgcc atttataaat gcaggagacg gcaaaaacca gcatccaact caaacactta
     3541 cagacttact tactataaaa tcattaaaag gaactttaga aagccataca ataggattat
     3601 gtggtgattt aaaatatgga agaactgtgc attctttggt taaggctatg gcaagatata
     3661 aaaatacaaa gtttgtattt atagcgcctg aagagttaaa aatgccagat tatataaaag
     3721 aagctataaa gggacatgca tattatgaaa ctaataattt ggatgatgtt ataggaagct
     3781 tggatgtttt atatatgact agagttcaac aagaaagatt tgaagataaa tcagagtatg
     3841 aaagacttaa aaactattat attttaaata aagctaaatt agaaaaggca tctaaagata
     3901 tgttagtaat gcatccatta ccaagagtaa atgaaataga catagatgta gatagtgatg
     3961 atagagctgt atattttaaa caagctaaat atggaatgta tgttagaatg gctttaataa
     4021 taaaactttt aggtataaat gaagactaat agttatcata taatagtaaa acaaaggaga
     4081 ttttgggatg tataaaattc tggaaaatat atatataggc gaagatatgt atagaatgaa
     4141 agtaaaaggt aattttgaag gtaaaatggg acaattctat atgttaagag catgggatac
     4201 ttatccagta ctttcaagac ctataagtat acatgatata gatgaagaag ggataacttt
     4261 tctttataaa gtagtaggag aaggaactca aattttgagt aacctaaagg taaatgataa
     4321 tataaaatta gaaggccctt atggtaatgg atatgctaag gttgatggaa aggtagctct
     4381 agtaggagga ggaatcggtg tagctccact atatttggtt gctaaaaata taaaaaattg
     4441 tgatgcatat cttgggttta gagaagatgt tatattagaa gatgaatata aacaagtatg
     4501 taataaagtc tatacaacag taggaaatac ttttgtaaca gatataattg atgttgagaa
     4561 atatgattat atattaacat gtggtccaac tccaatgatg gaaaaattag ttaagatggt
     4621 agaaggtact aaaacgagaa ttatggtatc tcttgaaaac catatggctt gtggagtagg
     4681 tgcctgttta gtatgtacgt gtaaaactaa tggaggaaat aaaaagactt gtaaagatgg
     4741 tccagtgttc tggggagagg acgtgatttt taatgggtaa tttaagtgtg aaatttggaa
     4801 gtgtagaatt taaaaatcca gtaataatgg cgtctggaac ttttggattt ggaaaagaat
     4861 ataatgagat ttatgatata caaaaacttg gtggtataag tagcaagggt cttactttaa
     4921 ataaaaaacc tggtaacaat ggtatgagag ttcatgagac atcatccggt atgatgaata
     4981 gtgttgggct tgaaaaccct ggagttcaag gatttataga ctatgaatta ccttttttta
     5041 gtaaactaga cttagtcaga atagctaatg ttggaggtgg gacattagaa gactacttat
     5101 taggggttca aatgttaaat gacaagccta tagacattat tgaattaaat atatcttgcc
     5161 caaatgtcaa agctggtgga atggcttttg gtataaaaaa tgaagtggca agagaagttg
     5221 ttcgtgaggt aagaaacata acgaaactgc ctttagtaat aaaattatca cccaatgcag
     5281 aagatattgt aggaatggca aaagtttgtg aagaagaagg tgctgatgga gtttcattag
     5341 ttaatacttt taaagcaatg gcaattgata taaaaaatag aagaccagta tttgaaaatg
     5401 tatatgctgg gttatctgga ccagctataa aacctatagc tttgagaatg gtacatgaag
     5461 tgtgtaaaaa tgtaaatata cctgtaatgg gaatgggcgg aataactaaa gccacagatg
     5521 ctatagagtt tataatggca ggagctactt gtatccaagt tggaactgca aattttatga
     5581 atcctagaat aggtattgaa ataatagatg gaattaatga atttatggat agagaaggaa
     5641 taaaaagttt agacgaaata agaggaatta tataaggtag aataaatagt attttaaaga
     5701 taaaaaatat agattaaata aacaataatt ttataacatt aacatggagg aaaaataata
     5761 tgagtaaagt agatgtagtg gatatattaa agaaaagtga tgcattgttg gagggacatt
     5821 ttttattatc ttcaggaaaa cacagtaata gatacgtaca atgtgcaaaa gtattaagat
     5881 ttccagaata tgctgctgag gtattaagta cagttgttga acaaataaaa gacttagata
     5941 tagacttagt agtaggacca gctatgggtg gagtaatagt ttcttatgag ttaggaagac
     6001 aattaggaaa agaagctgta tttactgaga gaaaagacaa tacaatggag ttaagaagag
     6061 gatttgaagt taaaaaagga gcaaagataa taattgctga agacgttgta actactggta
     6121 aatcaactat ggagacaaaa agagtattag aagccttagg tggagaagtt gtaggtgttg
     6181 catgtatagc agatagaact aatcatgata taggtatgcc tatatatagt gctataaaac
     6241 ttgatattca agtttatgaa tctgatgagt gtcctttatg taaggaagga aaattaccag
     6301 ttgttaagcc tggaagtaga gagttcaagg aattagggat gtaataaaaa cttaattatt
     6361 tatagtgtta cttaaaaaat gtaaattttt tagagtaaaa atttgtaata taacatataa
     6421 cttaaaaata aaaagcagtt agccttataa atgaaggtta gctgtttttt tgttaaaaat
     6481 atatttacaa aataagagat agtagcagta caatataagt ataaggtaaa agtattatat
     6541 attagggagg ggtaagtatg aaaaatcaat taaatgaaaa tcataaaaag gttgtatttt
     6601 ttggagtagg tgctgtagga gctacttttg cagaacaatt ttttaactct aaatatgatt
     6661 ttaaaattct ttgtgataat gaaagaaaga aaagatattt agaagaagga tttataataa
     6721 atggaaaaag gtatgatttt gattatgtaa ctaaagatga gtataaacaa gaggctgatt
     6781 ttataattat aggtctaaaa tataataatt taaaagaaaa tataaaagaa ttagatggat
     6841 tagttggaaa aaatacagtt ataatgtctc tgctaaatgg agttgatagc gaagagataa
     6901 taggagaaag atttggaatt gaaaaaatgg tatattcata tgttaccaat atagatgcaa
     6961 agaaaatcaa taataatatt atacacacta ctaatgggat aattgtattt ggtaacaaag
     7021 ataatagtga agatagaaaa actaatataa taaccgaagt ctttgatgat gtaaatatag
     7081 aatatacttt atcaaaagat attcaacgag atatgtggtg gaagtacatg gttaatattg
     7141 gtgtaaatca aacttcagct atacttggtg caccttatgg agtttttcag agttctgagc
     7201 atttaagaga attagcaaaa tctgcaatga gggaagttgt tgctatagca caagcaaaag
     7261 acatatctct tacagaagat gatgtggaac attcattaca tagaatacta gaacattcaa
     7321 aagaaggaag aacatcaatg cttcaagatg tggaagctca tagacttaca gaagtagata
     7381 tgttttctaa gaatatctgc aaacttggaa aaaaatataa catacctact cctataaatc
     7441 agactttctt ttatatgata aaagtaattg aaagtagatt ttaaagggtc tttgtaagaa
     7501 atattgataa ttattgattt taaattttat taatgttata tactatttaa gttactaaat
     7561 atttttaact cttcaatgaa aatagttgct gcttttgata aagttatatc ttttggaaat
     7621 atatatgaga aatgtctgtt gtagctgctt ccaagctcta tgacagacag ctcattgttt
     7681 agaacaggta agcttgtcac tagattagag actattgtaa ttcctaaatt atttctaaca
     7741 gattctttga cagcataatt acttccaaaa accatcatac tttttggaat aatttcttta
     7801 acacttaaaa acatatctaa ataatctctg gttccagaac catcctccct gactatccat
     7861 ttttggtttt gaagtttatc aaaactaaaa tcttttaata aatgactctt gtatggaagt
     7921 gctaaaacca tcttatcttc aaaaaaatat tcttgaataa atgaagggga agagcaagtg
     7981 ccttcaataa gaccaatgtc taaaatataa tccttaacat gagaagatac gattgatgta
     8041 ttttttataa aaacttctac atctatgtca ggatactttt tagaaaataa tgctagaaaa
     8101 tttggtaaaa tatactctcc aatagttaaa cttgcaccaa tctttaagtg accagttata
     8161 gcatctgaaa cttggcttac atcatgataa gtaatattta aaagatttaa tatttcttta
     8221 gctcttttgt acaagatttg tccactttct gttataaaaa tggttttttg tttaatagac
     8281 ctgtttataa gcacaacttt aaaataattt tctagatttt ttatgtgttt acttacactt
     8341 ggttgtgata gatttaaata ttccccagct ttagtaaagt ttttgtattc aacaacagct
     8401 acaaatgttt ttaattcttc aaacaatttt ttgtctcctt tacctaatga aattttacat
     8461 tttttatata ctttctacag ataattatac tatatataat aaccattaaa aacattaatc
     8521 attacgatat tatatattta ttttacttat ggttattaca attatatact attgctatga
     8581 agaaaactat attaattata aggtaggtat attatgttaa agaaaaaaag cagtttgaca
     8641 ttaaaaacta taagggaaat attaccagga ctgtttgtat cagtattagt aggttatatt
     8701 agtatattta tttctatttt aattcctaag gtaggggcag catcaatttc tatattttta
     8761 ggtatgtttg taggaaacct atttttaaat caaaaggtat ttcaaaaagg ttataaattt
     8821 tcagagacag atttgttatc atattcaata gtactattag gagcaacact cagtgtatct
     8881 accttaatag acttaaaagt ttcaggaatt ttatttataa tattgcaaat gacaataaca
     8941 ataatagctg cactttatat aggtaaaaaa ctaggatttg aagaaaactt tagatttcta
     9001 atggcaagtg gaaatgcagt atgtggttct tctgcaatag gagcaacagt tccagtggtt
     9061 aatgcgacag ataaagaaaa gggaattgca gtaactattg taaatgttac tggtatattt
     9121 cttatgtttt tacttccaat aatatctcaa tttttatata gccatgaact tgtaaaaact
     9181 tcagcaatga taggtggaac tttgcaatca attggtcagg ttgtagcaag tggagcaatt
     9241 gttggagaaa atgtaaaaga tttggctact atttttaaga tagtaagagt aatatttcta
     9301 gttgttgttg tattagtatt tggacatatg aaaaatcaat ctaataggga aattcttgaa
     9361 gaagaaaagg atgagataaa aaagaaaaaa gtgactatac catggtatgt aattggattc
     9421 tttataactt gtgcactttt ttcaatgaac attataccaa aagaagtatc tgtgttgtgc
     9481 aaagaaataa gtaacaaact agaaataata gctttagcag caattggatt aaaggttaat
     9541 gtaaaagatt tagtgaaaca agggaaagaa gtttctttat atggtttatt tgtaggtata
     9601 gtacaagttg tatcagctgt agttttaata aaaatattta tatagcataa aaatagtatt
     9661 tgtaatatta atttgattaa ctaaaataat atgatactaa taatttataa tataaataaa
     9721 aaatgaattt cgataagttt aagtgggtat aaacttatag aaattcattt tttatttaag
     9781 gtaaaaattt aaagtaaaat attattttac ataataataa ccttataaat tttgaaatta
     9841 cttatctttg tgttaaaata agtaaaatgc acatattaga gctaagtttt taagtaaata
     9901 aacaaaatga aaatataaat attaaggaga tttggtatat gaatgtttat gaaaaaggta
     9961 atggagttat actagaagga gtaacagatt ttgacccagt acatatattt gagtgtggtc
    10021 aatgttttag atggcacaaa caagaagatg gctcatacac tggagttgca aagggaagaa
    10081 tattaaatgt caaaaaagag aatgataaaa tatatttaaa taatactaat ttaaaggagt
    10141 ttaacagcat ttggtataat tactttgatt taggaactga ttatacagaa ataaagaata
    10201 agctaaaaaa tatggatgaa tatttaaata aggctactga atttggatgg ggaataagaa
    10261 tattaagaca agatggatgg gaaatgctta tatcatttat aatttcctct aataatagga
    10321 ttcctatgat acaaagagcg atagaaaatt tatcaagaaa gtttggtaag tatataggtg
    10381 aatatgaagg taatgaatac tatgcattcc caacaccaga agaactaaat aaggcctcac
    10441 aagaggaaat aagagcatgt caaacaggtt ttagagataa gtacataaaa agtactacac
    10501 aggcagttat agagaataat gatgaggtat ctgaatatac aaatttaagt acagaggatt
    10561 gtagaaaaga gcttttaaaa tttaatggag taggaccaaa agtatgtgat tgtattgcat
    10621 tatttggtat gcaaaaatat gattcttttc cagttgatgt ttgggttaag agagttatgc
    10681 aagaatttta tatagatgaa gacatgagtt tacctaaaat gagaacttat ggaatcgata
    10741 aatttaaaga aatgtctgga tttgctcaac agtacttatt ttactatgca agagaactcg
    10801 gtataggaaa ataaattaag ggggagaaat tatgggagta ataggaacaa tatttctgtt
    10861 ctatttaatt atatcatatc tagctggagc cataatttct gtgattatat tgcttgaaaa
    10921 tagagaccct gcaaaaacaa tgtcttggct attgatgttt attatatttc caggtgtagg
    10981 tttaatgata tatgctattt caggtagaaa tattagaaaa agaaaattat ttaagactca
    11041 aaaactagct aataatataa aagaaaagaa actatttgat acattagaaa agataacaga
    11101 aatagttgaa ttagaaaaag aatctataaa acaaaataag cttttaagag atgaagagga
    11161 tggtagttat agaaaaaggg taataaatat gttgttaaag actggtatgt ttccctttac
    11221 taaaaataat aaagtggatg tatttgtaga tggaaatgaa aagtttaaaa gacttataga
    11281 agatataaga gaagctaaag accatattca tttagagtac tttatcatca aagatagtga
    11341 aataggtagg gtgttaaaag aagaacttat aaaaaaggct aaagaaggca ttaagataag
    11401 aattttatat gatgatgtag gatgctggag attttggttt aatagaaaat tttttcgaga
    11461 aatgagggaa gtaggtatag aaattgcagc atttttacct acaaaatttc ctataatagg
    11521 agggaaatta aactatagaa atcacagaaa aattgttgtt attgatggaa taattggata
    11581 tacaggtgga ataaatatag gagatgaata tttaggcaaa aatgataagt ttggatattg
    11641 gagagatact catattagaa ttaagggtat ttctgtatac atgcttcaaa tgacatttct
    11701 gattgattgg tattatacga caaaagaagt attagtgact aaaaattatt tcccaagtgt
    11761 agggaatgtt ggtgaaagta tgatacaagt tgttgcgagt ggtccagaca gtgactggga
    11821 agatattcat tatgcttatt tttctgcaat atgtcaggct agaaaaaatg tttatataga
    11881 aactccatat tttattccag atgaaagtct actaaaagct ataaagagtg ctgctcttag
    11941 tggagttgat gtgagaataa tatttcctaa aattgcagac cacaagatag taaatatagc
    12001 atcttattca tattttgaag aaatattaag agcaggtggt aaagtttatt tatataataa
    12061 aggatttata cattctaaag tcgtaataat agatgataaa attgcttctg caggtacagc
    12121 taatatggat ttaagaagtt ttatgcttaa ctttgaggta aatgcattta tctatgatga
    12181 agaagttatt agggtaatga cagatgattt ctttgaggat ttaagtcact gtgaagaact
    12241 taatttggag gtatttaaga atagaaatat catacaaaaa atcaaagaat ctgtagcaag
    12301 attattttca ccaatattat aaaaaattat taaaaaaggt attgaaaatt atgtgtgatt
    12361 ggtatattat aataattgtt agcactctat tagatagagt gataataaat aaaaagtata
    12421 tatataaatc tacatatata agtagtatta atttaaaaat aactaaaaac ataggcgaac
    12481 tagtctatac ttaatatttt aggaggcgtg aataatgaaa ataagaccat tagctgacag
    12541 agtagtaatt aaaaaagtag aagcagaaga aaaaactgca agtggaatag ttttaccagg
    12601 agcagctaaa gagcaacctc aaatagctga agttgtagaa gttggaccag gtggaatagt
    12661 tgaagggaaa gaaataaaaa tggaattgac agtaggagat aaggttatat tccaaaaata
    12721 ttctggaaca gaagttaaga tagaaggaca agaatacaca atactaagac agagtgatgt
    12781 attagctgtt attgaataaa tatagaataa atttattagg aggggtttaa aatggctaaa
    12841 gaaattaaat tttcagagga aacaagaaga gctttagaag ctggtgtaaa taaattagca
    12901 gatacagtaa aagtaacatt aggacctaaa ggaagaaatg ttatattaga taaaaaattt
    12961 ggttctccac ttataactaa tgatggagta actatagcaa aagagataga actagaagat
    13021 agatttgaaa atatgggtgc tcaattagtt aaagaagtag caactaaaac taatgatgta
    13081 gctggagatg gtactacaac tgctacagtt ttagcacaag ctataataag agaaggttta
    13141 aagaatgtaa cagcaggggc taacccaata cttttaagaa aaggaataca aaaagcagta
    13201 acagtagcag tagaagaatt aaaaaatcaa tcaagaatag tagaaacaca agaggctata
    13261 tctcaagttg cttctatatc tgctggtgat gaagaagttg gaaaattaat agcagaagct
    13321 atggaaatag taggtaaaga tggagttata actgttgaag agtctcaaac tatgaatact
    13381 gaattagatg ctgttgaagg tatgcagttt gatagaggat ttgtttctgc atatatggtt
    13441 acagatgtag ataaaatgga agcagtttta aacgacccat atatattaat tactgataaa
    13501 aaaatatcta acatacaaga attattacca gttcttgaac aaatagttca acaaggtaaa
    13561 aagttattga taatagctga agatgtagaa ggtgaagcat tatctacatt agtagtaaat
    13621 aaattaagag gaacatttga tgtagtagca gttaaagctc caggctttgg agatagaaga
    13681 aaagaaatgc ttcaagatat agcaatactt acaggtgctc aagtaatatc agaagaatta
    13741 ggttatgatt taaaagaagc tgatttatct atgctaggta gagcttcatc tgttaaggta
    13801 actaaagaaa gtactacaat agtagatggt tctggagata aaaaagctat agaagataga
    13861 gtaactcaaa ttaaacatca agtagagcaa actacttcag attttgatag agagaaatta
    13921 atggaaagat tagctaaact tgctggagga gtagctgttg taaaagttgg agctgctaca
    13981 gaagtagagt taaaagaaag aaagttaaga atagaagatg ctcttaatgc aactagagca
    14041 gctgtagaag aaggaatagt agctggtgga ggaactgctt ttgttagtgt tataccagca
    14101 ataggaacac taattgagag tttagaagga gaagttaaac taggtgctca aatagttaaa
    14161 aaagcgttag aagagccatt aagacaaata gctataaatg ctggtcttga aggtgctgta
    14221 attgtgcaaa atgttgttaa ttctgaagca gaaactggat ttgacgcatt gaatgaaaaa
    14281 tatgtaaata tgatagaagc tggtatagtt gacccaacta aagttagtag aagtgcttta
    14341 caaaatgctg catcaatagc aagtactttc ttaactactg aatctgctgt tgctgacctt
    14401 cctgaaaaag aagatgcagg aatgccagga atgggtggcg gaatgcctgg tatgatgtaa
    14461 tttaaaaaat ttaaaatata aattttaata taaagaacta tttcgactta gattttaaac
    14521 tctaagttga aatagttctt ttgtatatat ctaatttttg tatatctata aattcaatac
    14581 acatatttag ataatcagta tctagtttgt aataatattt tagagtagta ttgttatttt
    14641 ttaggttatg attttagata tatattaatt aaggtataat tgttgataga gaataaatca
    14701 aatttaaaag gatgcgataa tatggactta agaaaattgc ttgaagaagt taaaaatgaa
    14761 aatattgata tagattttgc gttagaaaag ttaaaagatt taccttatga agaccttgga
    14821 tatgcaaata tagaccatca tagagaatta agaaatgggt atccggaagt tatttattgt
    14881 gaaggtaaga gtgatgagca tattttaggg attattgata aaatgaatca aaaaagttct
    14941 aacatattgg ggacaaggtg cagaaaagaa acttttctga aaattaaaga aatctataat
    15001 catgctgagt acgaagaggc gtctaaaatt cttaagattc aaaatcatga tatagagaat
    15061 ataggaaaag gtaaaattgt gattgctaca gggggaactt ctgatatacc agtagcagac
    15121 gaagcttatc atacagctaa gtttttaggt aatgatgttg atagaatata tgatgttgga
    15181 gtggcaggta tacataggct tttaaataaa agacataaaa tagatagtgc tagagtaata
    15241 gtagccgttg caggtatgga aggtgctctt gcaagtgtaa ttggtggact ggttgatgtt
    15301 ccagtaatag ctgttccaac ttctgttgga tatggagcaa attttggagg attagcagca
    15361 ttgctagcta tgttaaatag ctgtgcatct ggaatatctg ttgtaaatat tgataatgga
    15421 tttggagcag gatatttagc atcaatgata aataaactgt aaatacattg atataaatta
    15481 aacaaaacta tataataaaa tttaatttct attatatttt tactattaaa attgaggaga
    15541 catatatgag tgaaaaaata ctttattttg atataataag cggtatatca ggggatatga
    15601 cccttgcaag tttattaaat ttaggtgtgc ctaaggaaat ttttttggag gaaattcata
    15661 aactaagaat gagcgatgaa tttaatataa acatcagctc gaaaactgaa aatggaatag
    15721 taggtacaaa agtagaagta attacaaaag aaaaacatac acatagaaat ttagtggata
    15781 tatttgaggt tatagataaa agcactctaa acgaaaatgt aaaatctaaa gcaaaaaaaa
    15841 tatttatggt aattggagaa gcagaagcaa aagttcatgg aactacaata gacaagatac
    15901 attttcatga ggtaggggct atagattcaa ttgtagatat agtaggagct tctatattag
    15961 ttgacttact ttgtgtagat aaagtttgtg ctagtacagt gtctgttggt tcaggatttg
    16021 taaaatgtga acatggaatt atacctatac ctgcacctgc aactatagaa attttaaaag
    16081 gtgtgccaat taaattaaat aatgtaaatg gagaatgtac aactccaaca ggagctgcaa
    16141 tcataaaagt tttgtgtgat gagtttgtag atgagcttga attttctcca aagcaaattg
    16201 gatatggtat aggtcataag aagtttgaag taccaaatat actcagaact gtattaggtg
    16261 aaaaaaaaaa gaagaattaa tttatgaaat tagtgcaaac attgatgata tgtcttcaga
    16321 aatctattct tatttgtatg aaaaaatatt agatgagaaa gcattggata tttatacaga
    16381 gagcatttat atgaagaaga atagaccagc aacaaaagta tcaattttat gtagtgaatc
    16441 agatttagaa aaatttatta aaatattact tttagaaact agtacttttg gagtgagata
    16501 taatgcatat aatagagaaa ttctagagag aaaatttgta aaattagata caaaatatgg
    16561 agtggttaca gtaaaactag ggtattataa aggaaaacta gtcaaggcta ctccagaata
    16621 taatgaatgt aaaactatag caaaaaatga agatattcct ataaaaaaag tgtttgctga
    16681 tataaatttt agaataaaag aagaatttag tgaaaactta ttgacataag taataaattt
    16741 tgataaatta atattcagcc ttattaaata aaagtaaaca gaaaaaaata tataaaatat
    16801 aaactcactc gtatatgctc agaaatatgg tctgagagtc tctaccaaga taccgtaaat
    16861 atcttgacta tgagtgaaat tattatacca aaagacctag ttaagtaaat gtgtaggctg
    16921 agttaaactc cctatgtgtt ggctataaga taaaggtatt ttggaagcct gtatagtaat
    16981 ttcacttttg ctagaaatta tggaagttac tatacaggtt ttttgtgtaa aaagatttaa
    17041 tataatttag actgtattgt gttaaaataa atatataaaa actatattat acaaggagag
    17101 atgtgaattt atgaaacatg aattagtact tgtaatagat tttggaggac aatataatca
    17161 gttgatagct agaagagtta gagaaaacaa cgtatattgt gaaatacttc catgtactgc
    17221 aagcatagaa agaataaaag aaaaaaatcc taaagggata atatttacag gcggtccaaa
    17281 tagtgcgtat ttagaagatt ctccaacaat atcaaaagaa atatttgaat taggagtacc
    17341 aatacttgga atatgctatg gtattcaaat aatgtctcat gtattgggtg gagttgtaag
    17401 aaaaggtaat aaacaagaaa aagaatatgg aaaaacagct ataacttatg gaaaatcatc
    17461 attatttgaa ggaataacta caaatagtgt atggatgagt catacagatt taatagaaaa
    17521 agttccagaa ggatttacta tagttgcaaa tactaatgat tgtccagtag cagctatgga
    17581 aaatgtagaa cgaaacctat atggagttca attccatcca gaggtggaac actgtttgga
    17641 aggggataag atacttacaa acttcttata taatatttgt aaagtaaaag gtgactggac
    17701 tacagattca ttcatagaag ataaaataaa agaattaaaa gaaaaaatag gcgataaaaa
    17761 agctttatgt gctttaagtg gaggagttga ttcttctgtt gctgcagttc ttattcataa
    17821 agcaattggg gacaacttaa cttgtatatt tgttgaccat ggtttactta gaaaaaatga
    17881 aggaaatgat gtagaaagaa tatttagaga gaaatttgat ataaatttaa taagagttaa
    17941 tgcagaagat agattcttat ctaaattaaa aggagtttct gaaccagaag ctaagagaaa
    18001 aataataggt gaagaattta taagagtatt tgaagaagaa tcaaataaat taggaaaaat
    18061 ggatttctta gtacaaggaa caatctatcc agatgttata gaaagtggac atggaaatgc
    18121 tgctactata aagtctcatc ataatgttgg tggaatccca gaagatgtag acttccaaga
    18181 aattgtagaa ccattaagag agttatttaa ggatgaagtt agaaaaatag gattagagct
    18241 tggtatagaa gaaggattaa tatttagaca tcctttccca ggaccaggtc taggaataag
    18301 agttataggt gatgtaacta aagaaaaatg tgatatatta agagaagctg atgctgttta
    18361 tatggacgag cttagaaaag caggtttata cagagaaata tggcaagctt ttgcaacatt
    18421 accagatgtt aagactgttg gagttatggg tgatgaaaga acatatgctt atttagttgg
    18481 tttaagagca gttacttctt ctgatggaat gacttctgac tggtataaga tgccttatga
    18541 tgtattagaa agaatttcta atagaataat aaatgaagtt gatggagtaa atagagtagt
    18601 ttatgatata acttctaaac ctcctggaac tattgaatgg gaataaaata tagaatgcca
    18661 aaaatccaca taaatacagg atttaaggtc tctcttactt ctatgttata ctggtttgat
    18721 actatcaata tttgccagta tcttatggat taataagtaa aaaacatcct gctgaatgat
    18781 aaattcagcg gggtgttttt attttgtttt agaactcctt tgctctgaaa ctcgaagtat
    18841 ttaacactga tgattttgtt atcttttagt tccttttgtg tcatgctctg aaagacaaat
    18901 tgatacaata aaaaagtaat ttcttaatca ttgatggaga tatgtattta tgttactctt
    18961 gatggtaatt tgagtaatat tgttcatagc tttttatatt caattaatta gaaaaccatt
    19021 tggtataatc ataaataaac ttgatgatat aatagaaaag atttataatt attagaagta
    19081 tataaaagta ttttatgtct atgaaatgta tgtctataaa acatatagat gacttttgta
    19141 tattaatgta tgaatattga tattatagaa gaattgagtt gtcaatgata ctaaatcaaa
    19201 tatttataga agctggaggg attatgagaa ataaaataat tataggaatt acttttttac
    19261 ttgtattact aatgtctgga tgtgcttcaa aacaaaccgc agaaataaac gtattagcaa
    19321 ctactgattt acatggagtt ataccttatg agttaacttc ttatgtaaaa gaagaaagaa
    19381 aaaaagataa aaatctaact ttagtagatg caggagattt ttttgatagt ggagaagtgt
    19441 ttggttctag catggatagt tattttagtg ctagatataa agatggtgat aataacactg
    19501 aaagttatat tgagacgcct atttcaaaag atatgaaaga ggtaggttat gatgttgcag
    19561 ttcttgggaa tcatgaattt atatccaaca ataaatttta tctagataat atgatatctg
    19621 attttgagaa gcaaaaaata aatattttat cggcaaatac gtataaaaaa aatggagaca
    19681 gttatacaaa accatacata ataaaaaata ttaatacacc tgaaggaaac gtaaaactag
    19741 gaattttagg gcttactata aaagaagttg gtgagaggaa acagtggaaa gatggaaaac
    19801 ttgttgactc taagtcttta gaactaaaag accaagaggg ctacaatggt gaattgtata
    19861 tgaatgattt agtagaagat gctaaaaagt gggttagtat aatggaaaaa gagaagcctg
    19921 acattatagt tgctgtggca catacaggtg agaaacctaa gaaacctaaa aacccaggaa
    19981 atagaataca agacttagct caacaagttg atggaattga tgctatagtt gcaggtcata
    20041 atcatgttca aattgagcaa catgattata aaaataaatc aggagaaaat gttatagtta
    20101 ctgagccagg aaagcatggg gaatgtattt ctaagattaa ttttaaatta gaaaaaaaca
    20161 atgataaatg gaatatagtt gacaaatcta gtaaattaat taaatttgaa aaaaatcaag
    20221 aaagtgataa atttggagaa tttatttctt caattaataa agtaagtaaa gataagaaag
    20281 aaataagatt atcagaagta aagactcctg aatgggataa aatatatgtt tttaaatctg
    20341 gaacatctgc agatgatata tataaaacaa taggatataa atggagaaat atatcatctc
    20401 caaagagtga tggcatggtg cagattgttg taatgaataa taataagcct gttagctata
    20461 tatatggtga agctgaagtt atgccaatta atataaaatg tgatgaatca gagtataaag
    20521 ataatgttgt aactatttat cctaataaaa atgataaatt taaaattgaa aaaggtaaac
    20581 aagaatatag tattaattta aaacatatac aatagaaact tttatcttat ttaagtttag
    20641 attgtactta aaataaagtg tgtattattt taaaaattga tgataattaa aatcacaaca
    20701 ttaaggaata gtacacactt tttatgctaa aaaatagaat gttaaaaaat taaaccttta
    20761 aattaaagta cattgcttat attatccaat attatcttca aaggttgtgt aatattttta
    20821 tccttatgta atattgctaa cattttattt tccatatcta tcccatctaa ttcaatcaat
    20881 cgcaattttt tatgtaagag tgaatctaat aataaatttt ctggcaaaat ggttatacct
    20941 aaccctgctt gtgcagcttt aataagtgca aaagaattaa cactttccca aacaggataa
    21001 gctttttggt ttgaaagaga taatacactg tccaaagtat cgcgaatggc acttccttgc
    21061 tcacgtaaca ataacggaaa cgtgcataac tgtcgtagag ataaggcttg tttagtattg
    21121 gtggtggtta tgcaatcata cctttaatcc aagagaaagt agtaaatcaa actggatgga
    21181 taagtgagaa aatatttaca gatattataa ctatttcaca aatgacacca gggccactgg
    21241 ctgttaatac ttctactttt gttggacttc aaattggagg gttttgggga gcagttgcag
    21301 ccactttagg ttgtgtatta tgtggtgtag taatatctct atttttatat aatttttttc
    21361 aaaagcatca gaactctact tatatatttg aaatattaaa tggtctaaaa tcagcttcat
    21421 taggtttaat tatctcagct gcggctacaa ttataattct tgcattattt gggacgaata
    21481 aagtgtactt caattctata tttgagatgc taaactggac tgcactaata atcttttttg
    21541 ttacactact gatagtacgt aagtggaaaa ttaatcctat tgtgattata ataatgagtg
    21601 gtatactggg aatatttttt tatttataag tttttgctaa gtaaaggata tctatttttt
    21661 tatatcttaa atccaaatag ccaataaagt aaggctattt ggatttttaa taaattctga
    21721 aaagataatc agaaacctat ataacattta aattatatca ctggttttct acaaaccaat
    21781 cataagcttc ttggcaaaat ttcttactat ctatatagta actattatga acattattaa
    21841 tttcaacaag tttcttttta cattttagtg aattataata gctatcaaaa tattgggttt
    21901 taaaatatct atcatgaagt gttttaaaga caagagtagg tatgttgact ccatctttta
    21961 tcttactatc ttttgcaata aagcattcta acattgtatt aattatcttt gtactatttt
    22021 tccaaatatg aggatttttc ttttcaaata ttttataaaa ttcattatca tagacttcat
    22081 tataaccttt atatttaggt ggttttactg ttatgtgaaa attaggaaga aacttaaaga
    22141 aaaaatataa taatttttat gttctgtttt atatattatt catattttat cacagataat
    22201 atactaaagt taattttttt attgtacaac tattataata attcttctat aatataagac
    22261 attctataat tttatattta agttcatgtg tatttaattg tttttatata aaatgtccag
    22321 taatactatt tggatttatt tttatttcat caggagttcc agaagcaaca atttcccctc
    22381 catttattcc actgtctgga cctatatcga taatccaatc agaagcacgt ataacctctg
    22441 tattgtgttc aataattaaa agtgaatgct gttcatttac aagttttcta aaagtaatta
    22501 acaaattttc tatatcttta aaatgaagtc cagtagtagg ctcatccaat atgtacagca
    22561 ttcgtttttt agtttttgtc cctaattctt ttgctaattt tatcctttga gattctcctc
    22621 cacttaatcc taatgtattt tgtccaagct ttagatatcc aagtcccact tcatctaata
    22681 aggagagttt tctatatata tcagagcaat cgctaaaaaa tgtttttgct tctgctactt
    22741 ccatgtcaag tatatctgca attgtctttc ccttgtattg tatagacagt atttcttttt
    22801 gatatctctt accttgacat tcactacaag ttacccattg gtctgccata tattgcagaa
    22861 taatttttgt tttacctaat ccttgacagt ttgggcattg cccctttttg ctattaaagc
    22921 taaaatagct ttcatccaat aatttttgtt ttgcttgtgg agtttcagca aatagagaac
    22981 gaatttcatc aaaaatattg atatatgtag caggtgtaga acggctagat tttccaatag
    23041 gtgcttggtc cattaagata aaatcatcaa tagcatcaaa tccagtaaat tcagtataat
    23101 ttttatctgg gatagatttt tgtttcaatt tttcttctag agcaggaagt aaactgtgaa
    23161 atacaagaga gctcttacca gaaccactaa cacctgtaat agagcacata cagtttaagg
    23221 gaatttctac atcaatattt tttaagttat ttgcatggca tccttttaag cttaaccatt
    23281 tacttggtat aattttattt tgtgaactta ttttattgta tgtacttaaa tatttacctg
    23341 ttatagagtt tggatttttt ataatttctt gggttgtacc ttctgcgata atgaaccccc
    23401 cttttgtacc agcacttggg cctacatcaa taatataatc agcagacagt attgtatctt
    23461 tatcgtgttc cacaactata acagtattac ccttatctct tagctcttct atcattttga
    23521 tgattaaatt atggtctctt gggtgtaaac caatagatgg ttcatctaaa atgtaggtta
    23581 agcctactaa ttcacttcct agctgacttg ataatcgtac tctctgcagt tctcctccag
    23641 ataaagttgg agctgttcta tctgttgata tatagctaag acctaccttt aatagataac
    23701 taactcgaat ttttaattct gttaagatat catttaccaa agagagctca tttttttgaa
    23761 ggtgatttgg taattcacac aaccaattcc ataactgttc tatcgtcatt tttgttagtt
    23821 cagggaagcg ataacctttg attgtagtga atctagcctc aatgcacagc aattcgccac
    23881 cacatgtatt gcaaggaatt ttgttcatat attgatgcaa ggtattattt tcagaactag
    23941 attctctaaa aagtctttga atgtggttga ttgctcctga agcaggtctt ctaatctttg
    24001 attctcgtcc ctttgattca aagctaaatt catatatctt atcgtctgta ccatataaga
    24061 ttgcatctat aaaatctctt ggtaattcat tccaaggtat atctaggtct atatttttat
    24121 cttttgctat tgcatataat tcacctacca tccaattacc atttggcttt ttccccctca
    24181 attttccaaa atagtaagta gctccatcta atattgaaat agttgggtct tttacaatta
    24241 attgaggatt aacttgatag gtaaaaccta atccattaca atcgtgacaa gctccagtat
    24301 gggagttagc attgaaggtt gaagtattta tcttagaaaa aatagtttga caatgtggac
    24361 aataattttt tgtattattc tttttttcta atggaatttt gcaatatgga cagtaacttt
    24421 ttccgattga tacaaataac attcttaaat aatcccaaat gcttgtaaga gtaccaacag
    24481 tagagcgagg attttggctt ccttttttct gttcgatggc tatagtaggg gttaatccct
    24541 gaagtaaatc aaattccggt ttttgaagct gttctgaaag ttttgctccg ttacttagat
    24601 tttcaaaata acgtcttttt ccttcttcat aaatcgtatc gaaagctaaa gaagattttc
    24661 cactaccact tacacctgta ataacagtta tttgattttt tggaattgta acttcaacat
    24721 cctttaaatt gttttgcctt cctcctttta aatagataga gcttttatta tgacaaagtg
    24781 aatttttaat tgatttattt attgcttttg taaatttgaa tttcttttct agttcaagag
    24841 gattgcctaa ttcttttatt tccgaaatac ctaatatttt taactgagaa aagattgatt
    24901 tcaaataatc ttcaggcaat ttttctatta acaaattaag ggcttctatt tttatatttc
    24961 cgcttgataa tatctctgcc atttcatcta ataaattttt ttctgctctt gttaattgat
    25021 tgtatttttt ggaacttaat tctgtccata aatcccatgg aagcaattct aaaccaatca
    25081 tattattaaa gtcatgtaga agattaccct ttacagaatg ccaacctttc caatttttat
    25141 tatatccaaa gtcatctgga tgagcatgtc cttgccgaca tcttatccag gcttctgaac
    25201 cagttaaaaa accatctgtt tttccgacat catgagtgtc aaaattaatt tgaaaataat
    25261 ttttttgtat gtcatctatt tgtgcatcta ttcttatcca tctctttgtt agagtatcat
    25321 agtattctac aagccagtgg tcttcataag taagctcact ttcaaaataa ttagcaaaac
    25381 ctgctctcat tctagcctca aaacctcgat atcttaggag cgatacaaga agtaaagaat
    25441 aatctctgca cattccaaca agcctttgtt ttgggtttct tggaatagtt agtgggttag
    25501 aatttatttc aaatattctt tttaacattt gaggaattgt acgaagcaat tcctcatcag
    25561 attgttgctt attaaaggat ataccatata attttccttg gtctccatga attactaggc
    25621 tttgaaccac ttttaataac tcaattggag tctttggtaa atcttgatat ttattttcaa
    25681 attttttagg atttgtgatg atactttgat ttttatagtt atttattttc atttatattt
    25741 cacctctcta aatggatgat ataccctgac atggtgtcat atgcaaggac aatttagatt
    25801 tttggccaaa atttaaaaaa taaataaaaa atgatacagt gataaattgt aatacaaata
    25861 tagtttcaaa ttgcatataa tataattatt taataaaacc attatctatt gaatgttaat
    25921 gaaatgttaa gatggcattc ttgaatatta agaaatagaa tactataata tagtgtgaag
    25981 gatttttagt aaaaaaattt gtaagtgtta atatacatta aaaaataggt ttataatttg
    26041 gaggaaaaaa atgaaattat caaggtataa gaaaaatagt ttagttctgt ctataatttt
    26101 atgttcactt attatttcaa tccttgtaat tactgttttt aataccgtaa aactacacaa
    26161 tatatctaat aaacaatcta aaaactattt aaatgatgta tctacacaga ttgtaatgaa
    26221 tgttgattct aaaatcaaat ttattttatc cgacttaaga ataatggcag attttattaa
    26281 gcaatatgaa ggtgatagtc gatatgagta tttatctaaa agaaagacta gttataagta
    26341 ttatgatgtt ggaataatag atttagaagg taaagctgag tttttaagtg gaaaaaagtt
    26401 tgacttaaag aatactgttt cctataaaaa aggaattgaa ggtaaagaat actcagaagt
    26461 aatagagtca atgggttttg ttctttatag tgtacctata tttaatgata atcatgaagt
    26521 atctagtatt ttaataggtg tatccaataa agaaagtatg aatgagattc ttaatataga
    26581 taactttaat ggaaaaggaa ctattgaaat tattaatagt aagggaaaac ctcttttcat
    26641 aggaaaaaat actgaattaa taaaggacct tagccataaa tataagagtt ctggagatga
    26701 accttgggca caaaagatgc ttgaggattt taaaaatgat aaatcaggga atattacaat
    26761 aacctcatca aaaggtgtac aatgcttatt aacttatcat ccaattacaa gaggtttaaa
    26821 tgattggcat tttcttctca tagtacctga agatgctgta cttggtgaac ttaataaatt
    26881 aaactctttt acaataacaa tgacatttat aattacatgt attattggaa ttataacatt
    26941 aattttatat attataagaa gaaaatatgt aaatcaaata gaaaatattg catatttaga
    27001 tagtataaca aatggtataa attcaactaa gtttagtatg ctagtcaaac ctttaatttc
    27061 agaatcacct gatagtactt atatgatgat agcaatgaat ataaaagatt ttaagctaat
    27121 aaatgattgt ttcggtagtg aaaagggaaa tatgacacta aaacatcttt acaatatttt
    27181 gtataaaaac attgatgaaa atgaagaatt tgtgtgccgt catgatgcag accttttcta
    27241 tttgcttatt aagaatagac cagtgttaga agccctggag ttattatata aaatagaagc
    27301 tgacacaaat tattttaata aaaataggga aaatccatac tttttaagac tatcagtagg
    27361 tatttacaca attgaaaatc ataatgaaga tttaattaca atacaagacc atgcaaatac
    27421 agcaagaaaa agctttaata aaactcatca aagtgatttt tctttttata gtgatataga
    27481 aagaaaacga ttaatatctg aaaaggaaat ttcaaattta atggaaaaag cccttgaaaa
    27541 taaagaattt tttatgtgct tacaacctaa aattgatata aaaagtggaa aaattagtgg
    27601 agcagagtct cttgtgcgtt ggaaaaattc agaaaagggc ataatttatc caagtgattt
    27661 tattcctctt ttcgaaaaaa gtggatttat atgtaagtta gatttatata tactagaaga
    27721 aacatgcaaa ttgatttcta agtggataaa agaaggtaaa gaagtgttga gaatttctgt
    27781 taatgtatca cgtcaacatc tgaatgataa attgttttta gaaaaatata agcatatatg
    27841 caataaatac aatgtaccaa catgtctgat agatttagaa cttaccgaat ctattttctt
    27901 agaaaatcca gaagctatag atataataga ggatattcat tctaatggat ttaaatgttc
    27961 tatagacgat tttggatttg ggtactcatc attaggaata ttaaaggatt ttaaagttga
    28021 cataattaag ttagaccgtt cattctttgt tagcaaaaat aatatagacc gtggtaaggt
    28081 agttattaag tctattattg aacttagcaa aaggctaggt atgaaagtaa ctgctgaagg
    28141 aattgaagaa ttagaacaag ttgagtttct aaaaaatgtg ggatgtgatt atattcaagg
    28201 ttatgttttt tcaaaaccat tattaatttc tgattttgaa aattttgcat atgaaaataa
    28261 taaaattaaa aatatccatt attaaatgta caagataaaa aatttaggca agttatataa
    28321 ttaaaaatat aacttgtctt ttttatttat atatttaata aaaatatatt tgcagatttt
    28381 gtaaaaataa ttttcaaatg ttgtaagttg tggctaaagc tttattgtat tattctgaat
    28441 atagaaagat agttactgtc ttagataaaa actgaaacat acataataat ataaaatttg
    28501 ctatattaat aactctaatt attttggaag gagggaaaat atgataagtg ggagaatgtt
    28561 actcatcatg aattttctaa aaggaaaaaa taagacaagt tacaaggata ttagcaaaca
    28621 acttagtata gaagaaagaa aagttagata tgatataaat aatttaaata ttgttttgcg
    28681 ttcattaaac aagtcagtaa tacaaaagat gaacaaaggt gtgttaatta ttcctgatga
    28741 cttccaagta gtatgtataa ataataacga atatgtattt tccttaactg agcgagtatc
    28801 tattatgaaa attatatcaa tgtttaaaat agataatcta aacttagaaa aattaagtaa
    28861 agaatttcag gtttctagaa gttcaattaa aaatgacctt aatgttttgt caagtgaatt
    28921 agaaagaaac caaataacta ttacttatga taaatctttt aaaataagtg gagaagaaga
    28981 cattatatat aagcaaagat tgaacatatt aaaatcgtat tcttatcttt ttggtaagga
    29041 aaaatctgat ttaagtgtat ttgagaagta tttagtgaat gtaattgaaa aaatattcaa
    29101 agggatttat ttgattgata tatataattg gtctatgaat ttattattgc aaatgggatg
    29161 gactttaaat gatgaatctt ttacatggta tgtgtcaaac attttccttt ttacatggta
    29221 tatacattca gatgaaaaac atcctttaga atctgcttca ttaagtgagc cgtgttttga
    29281 aaataagttt gaccaagaat ttgaaacaat tattcaaaaa aagttgacta aggaacaaat
    29341 ttttctgtta aagagttttg tattttttac aaataaatat gcaagtttaa acgaggatat
    29401 ggatttgatt agtacagaaa ttatagttca agatctaatt tacaatatgt cagaaatatt
    29461 agagattagc tttaaagaag atatgattct ttataaagga ttactcaatc atattgcccc
    29521 attgatagag agaatcaaac agaatataca gatttatgag gaattacttc atgtaatacc
    29581 agataaatat caatatgtac ttgaagctac taaggtttct attcaaaaaa acagagtttt
    29641 aagtcaaata aagaatgaaa atgaaacaat acttttggca atacatttct taggaagtat
    29701 acatagaaat gagaggaata actacaaaaa tatattgtta gtttgtggat ttggatatgg
    29761 tgtaattgct atgttaaagg atagtttagt aaatgattac cagataaata tagtagagag
    29821 tattccatca tataagttac aagattataa agaatgggaa agtattgact tcattataac
    29881 aacatcaaag atagtaatag acttgcctaa aacaattatt gagataaatc catttttaca
    29941 agaagaggat tatttgaagt ttgaagaaaa agggataaaa agaaagaacg tcttaactaa
    30001 ttatatgtca ataaaaaaaa ggttggactt tttagaagaa gatgcccaaa aaagagtatt
    30061 aagtattata tgtcaagaac ttggatacag tgatgagaga atattattta gacaacttaa
    30121 tttaagtgat ttaataggaa ttgataccat aaggataatt gatggagata tgaaatggga
    30181 agatgctgta aaaattagtt caaacatact tgctaattgt gattttgtat cagcacaata
    30241 tgctgagaat attatagata ttttaaaaaa tgtgggtttt tatgctgtaa aagataacga
    30301 atttgcttta ctacatggca atgatagttc attagtcaaa gtaagttcga taagtttatt
    30361 aatatgtaaa gaagcagtta gttttggaga taaaaaggtt aaaatcatat tttgcctagc
    30421 aagcagagat aaaaaagagc aaattcctgc aataattaat ctaactaaaa tggtttataa
    30481 gactaatttt attgctatgt tagaaagtgc aaagacaaaa gaagaagcag aagttttaat
    30541 tcataaatat gagaaggagg tcactgtatg aataaattag ttaatgtaaa atgttcattt
    30601 aaaatacatg ttaattcaaa agaagaagcc attatatcgt tagttgatgt agtgtcaaaa
    30661 gaaggatatt taatagataa aaatcaattt ttaaaagatg ttttaaagag agaagaaaca
    30721 ttgtcgactt atattggaca tggtataggt cttcctcata gtcaaagtgt aggtgttaaa
    30781 aactcttgta ttacaatagg aaagcttgat actcctattg aatggacaga agaaggagaa
    30841 aaagtggatt taatattttt gatatcagta acaaaagaca atgaaaataa tcttcattta
    30901 aagattttat caaaattagc acgtttattg atgcatgaaa gttttagaaa tcaaattaga
    30961 tgttcagatg aacaaactgt ttataatctt ataaaagaaa aaatagagga ggaagattaa
    31021 aatgagaatt ttaaagtttt tgcaaaaaca tcttatgaca gcaacatctt atatgattcc
    31081 atttgtggtt gcaggaggaa tattatttgc tttatcagta actttaagtg gacaagctgc
    31141 tgttcctgaa acaggctggt tggctaaact gaatcaaatt ggagctgctg gcttggcttt
    31201 attcattcct atattaggtg gatatatagc atttagtatg gcagataaac caggattagc
    31261 tccaggtatg ataggtgcat atttagcaaa agaagtaaat gcaggtttta taggtggtat
    31321 catagcagga tttatagcag gatttgttgt tttacaatta aagaaaatta aattagctcc
    31381 tacaatgaga actttaggtt ctatatttat ttatccttta gttggtacat tgattacagg
    31441 tggtataatt gtatttttaa ttggagaacc tatagcttct tttatgacat ggatgacaaa
    31501 ctggttaaat ggaatgtctg gtgtttctaa aattccttta ggtggaatct taggaggaat
    31561 gatagcctca gatatgggtg gccctataaa taaggttgct gctacttttg cccaaactca
    31621 agttgacaca cttccatatt taatgggtgg agttggcgtt gcaatttgta tacctccaat
    31681 tggtttagga ttagctacat tattgtttcc aaaaaaattt agcaaggaag aaagagattc
    31741 aggtaaagct tcactattaa tgggatgtgt tggaattaca gaaggagcta ttccatttgc
    31801 aacatcagat ccagttagag taataccttg tattatggct gggtcgattg taggaaatat
    31861 tatggctttt ttattaggat gtttaaatca tgcaccctgg ggaggtctga tagtgttacc
    31921 tgtagttgac aataggttgg ggtatattgc atcagtatta actggagcag tagttgttgc
    31981 ggtattaatg aaattagtaa aaaaagacgt gaaagaagat gaagaaattg aagaagattt
    32041 agatgaatca attgaattaa tatttgaaga attataaata taaatggaag gaatttgtat
    32101 aaaaaagtaa atataaaact ataaatttaa aggagataag aatatgaaaa ttgtagcagt
    32161 aacatcttgt cctagtggag tagcacatac ttacatgtcg gctgaatcac ttgaattatc
    32221 agccaaaaaa tttggtatag aaataaaggt tgagactcaa ggaagctctg gaatagataa
    32281 tgaattgtca cttaaagata ttgatgaagc aacttgtgta atattgacta atgatgttga
    32341 gattagaaat atggaacgtt ttaaaggaaa gaaggtagta agaatgagtg tatctgagat
    32401 aattaagaaa tctgatgcat taattaagaa aataaaagat acttttcaat aaaaattgat
    32461 ttattttaca tataactcaa agatgttttt aaagaaattt ttattaaaag catctttgaa
    32521 aatatcttaa aaatagaata aaaggagata tagtcatgaa aaagttacca ataaagactg
    32581 tcgtgcaaag attactaaac ttacaagaag aggggaaaag tgcaactctt ttaggaattg
    32641 gtcctatgtc accaaacttg ttacaagcaa gttttgaact agcaaaagat gatgattttc
    32701 ctttaatgtt tatcgcaagt agaaatcaag tagatgcaga tgaactaggt ggcggttatg
    32761 taaatggttg gaatcaggaa acttttacaa aagatattaa gaaggttgcc gacaaagttg
    32821 gatttgatgg tttatattat ttatgtagag accatggtgg gccatggcaa agagataaag
    32881 agagaaatga ccatttgcct gttgatgaag caatggagtt aggaaaaaaa tcttatttag
    32941 cggacattga agcaggtttt gacctattga tgattgaccc tacaaaagac ccatttgaaa
    33001 ttggaaaagt aattccacta gatgtagtac tagaaagaac tgtagaatta attgagtatt
    33061 gtgaaaacga gagaaaaaga cttaatctac cagacatagg ttatgaggtt ggtacagagg
    33121 aaaccaatgg aggtctgact tcaactgaga cgtatgaaac atttataact agactaaagg
    33181 ttgaactcga taatagaggt ttgccaatgc caacatttat agttggtcaa actggtactt
    33241 taactagaaa gacagaacaa gtaggaactt ttaattttag aaatgcttat gatttagctc
    33301 aaatggctaa gaaatatgga gttggtttaa aagagcataa tggcgattac ttagatgatg
    33361 taactttatt agaacatata ccatctcaaa ttattgctac aaatgtagct cctcaatatg
    33421 gtactgaaga aactagggca tatttaaaat tagcagaagt tgagtgtaaa ttagaaaaag
    33481 agggcttagt tgagaaaact tctaatatca gacatgttct tcttgttaat gcaattgaat
    33541 gtggacgttg gagaaaatgg gtagtaggag aacaaaaaaa tctaactact gaagagatat
    33601 ttaaggatga agttttatct aatgaaatac tagatattgc aggacattat acatttaata
    33661 atgatgatgt taaaaaagaa atagaagtgc tttatgataa tttaagtaag aataatattg
    33721 atggtcaaag atttgttgta gaccatatta aacgtccttt gcgtgattat gcagaatgct
    33781 ataacttaaa aggtgttact acgaggattt taaataaata ataggactta tagtggtgag
    33841 tattgttata ctaggtattt atagttataa ttatacttac cactattaaa tttaggagga
    33901 ctaatatttt gattaattta atttttgatg tagatgatac tttatataat caattaacgc
    33961 ctttttacac tgcatataat aaagtgtttt cttcaataaa ggatatttct attgaagatt
    34021 tatatatgag tagtagaaag tatagtgatg aggtatttca tatgactgaa aatggagaaa
    34081 tgccaatcaa agaaatgcat atttatagga taatgaaagc ttttgaggag ttgggaaatt
    34141 ctattacaga aaaagatgca caaagttttc aagatgagta tatatatcaa caatcacaaa
    34201 ttacactaat accagaagtt gagaggattt taaatttttc taaagaaaga aatattaatc
    34261 taggaattat aactaatggc ccatcaaacc atcaaagaat gaagttaaag cagttaaata
    34321 ttgaaaactg ggttgataaa agtaatattt ttatttctag tgaggttgga ttttctaagc
    34381 cagatactaa tatttttaga gtagctgaga atgttatgaa tttagataga gaaaatacat
    34441 attatgtggg tgattcttat agaaatgatg tattaggtgc taaaaaagct ggttggaaga
    34501 gtatatggtt gaaccatagg ggacatgagt tagaagagtt gttttataaa cctgattttg
    34561 taattttgga acataaggat ttgatatctt tatttataaa aatatgtagt tataaaaata
    34621 tgtagtaaaa aaatgttaat aatagaattg tataaaataa ttaataataa ctatataaaa
    34681 ttatatgttt agattgtgag caatctattt tatgttaaaa atagattatt tagaagaagg
    34741 ggaaatctta aaaaaatttg aatatcccct ttttattatt taattaaaat gaaatagtta
    34801 gaaatatatt ttatttagtt tagtcaataa cattttggga ttctttataa ctacatgtta
    34861 actgttgaat aggtttatca tatggtttaa cccatccttt attaatactt aattctgtaa
    34921 gtgctgtgtg accttctacc atttgagtta atgaagctga atatatagct cttagttcag
    34981 gtgtactact tgtcaatgca gaattaagat acatattggc agatgcttta gcagatgaaa
    35041 gcatacttaa cattattact ttatcgtcta tataagtatt gttcttaatt agattttcta
    35101 tcatatttct catgttatct atacctcctc agaaattagt atttcatttt caactataaa
    35161 ttgttgtata gctttaattc ttccttctgt tgctaatata ctagattctg attgtctttt
    35221 tagatcttca tctgatatta acatatttat tgctctttgc ataagtaatc cgtcagactc
    35281 catttttata attgctgcta aagatatttt ttcagtttct gaaagtattc tcatttaatc
    35341 atctcctata ttgtaattta tgctttaata gtatgtcttt cttttcaagg aaataaccat
    35401 tacacttttt tacagtattt tatattctcg ttatatcaaa atttagtcga cttaatatat
    35461 taatataatt ttgtaggata tttatcatta aatatctata aaataaattg tttgaatatt
    35521 cataaagcaa aatttatttg tatgtacttc ttcaagagta aatatgagtt ttttcaaaaa
    35581 attaattttt atgataatta ttaagggcta tatttttctt aaagttgtga ttatcattat
    35641 taatttacaa taaaattgcc gaaaagccct ctcctttagg tgtagggatg aaaggtaagg
    35701 tcttaatata aaaaagttag aataatgctt gtaatctaat accgcta
//
LOCUS       DAELTA010000022        34623 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.022, whole genome shotgun sequence.
ACCESSION   DAELTA010000022 DAELTA010000000
VERSION     DAELTA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.022"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(108..1271)
                     /locus_tag="KPG13_000937"
     CDS             complement(108..1271)
                     /locus_tag="KPG13_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0984678.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(1325..1525)
                     /locus_tag="KPG13_000938"
     CDS             complement(1325..1525)
                     /locus_tag="KPG13_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG0984679.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(1731..3407)
                     /locus_tag="KPG13_000939"
     CDS             complement(1731..3407)
                     /locus_tag="KPG13_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG0984680.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            3624..4361
                     /locus_tag="KPG13_000940"
     CDS             3624..4361
                     /locus_tag="KPG13_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0984681.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(4478..4936)
                     /locus_tag="KPG13_000941"
     CDS             complement(4478..4936)
                     /locus_tag="KPG13_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0984682.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(5218..5505)
                     /locus_tag="KPG13_000942"
     CDS             complement(5218..5505)
                     /locus_tag="KPG13_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG0984683.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(5583..6008)
                     /gene="ruvX"
                     /locus_tag="KPG13_000943"
     CDS             complement(5583..6008)
                     /gene="ruvX"
                     /locus_tag="KPG13_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG0984684.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(6214..8385)
                     /locus_tag="KPG13_000944"
     CDS             complement(6214..8385)
                     /locus_tag="KPG13_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984685.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(8395..8652)
                     /locus_tag="KPG13_000945"
     CDS             complement(8395..8652)
                     /locus_tag="KPG13_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG0984686.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(8677..11316)
                     /gene="alaS"
                     /locus_tag="KPG13_000946"
     CDS             complement(8677..11316)
                     /gene="alaS"
                     /locus_tag="KPG13_000946"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG0984687.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(11384..11622)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11765..12844)
                     /gene="mnmA"
                     /locus_tag="KPG13_000947"
     CDS             complement(11765..12844)
                     /gene="mnmA"
                     /locus_tag="KPG13_000947"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG0984688.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(13133..13573)
                     /gene="nifU"
                     /locus_tag="KPG13_000948"
     CDS             complement(13133..13573)
                     /gene="nifU"
                     /locus_tag="KPG13_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG0984689.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(13627..14820)
                     /gene="nifS"
                     /locus_tag="KPG13_000949"
     CDS             complement(13627..14820)
                     /gene="nifS"
                     /locus_tag="KPG13_000949"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG0984690.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(14838..15284)
                     /locus_tag="KPG13_000950"
     CDS             complement(14838..15284)
                     /locus_tag="KPG13_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0984691.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(15393..16163)
                     /locus_tag="KPG13_000951"
     CDS             complement(15393..16163)
                     /locus_tag="KPG13_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0984692.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(16194..17459)
                     /locus_tag="KPG13_000952"
     CDS             complement(16194..17459)
                     /locus_tag="KPG13_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG0984693.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(17566..18351)
                     /gene="codY"
                     /locus_tag="KPG13_000953"
     CDS             complement(17566..18351)
                     /gene="codY"
                     /locus_tag="KPG13_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG0984694.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(18516..20603)
                     /gene="topA"
                     /locus_tag="KPG13_000954"
     CDS             complement(18516..20603)
                     /gene="topA"
                     /locus_tag="KPG13_000954"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG0984695.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(20636..21736)
                     /gene="dprA"
                     /locus_tag="KPG13_000955"
     CDS             complement(20636..21736)
                     /gene="dprA"
                     /locus_tag="KPG13_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG0984696.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(21755..23356)
                     /locus_tag="KPG13_000956"
     CDS             complement(21755..23356)
                     /locus_tag="KPG13_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG0984697.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(23387..23731)
                     /locus_tag="KPG13_000957"
     CDS             complement(23387..23731)
                     /locus_tag="KPG13_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG0984698.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            23915..25078
                     /locus_tag="KPG13_000958"
     CDS             23915..25078
                     /locus_tag="KPG13_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0984699.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            25071..25718
                     /locus_tag="KPG13_000959"
     CDS             25071..25718
                     /locus_tag="KPG13_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984700.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            25847..26782
                     /locus_tag="KPG13_000960"
     CDS             25847..26782
                     /locus_tag="KPG13_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984701.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            26796..27893
                     /locus_tag="KPG13_000961"
     CDS             26796..27893
                     /locus_tag="KPG13_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984702.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            27896..28960
                     /locus_tag="KPG13_000962"
     CDS             27896..28960
                     /locus_tag="KPG13_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984703.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            29204..29701
                     /locus_tag="KPG13_000963"
     CDS             29204..29701
                     /locus_tag="KPG13_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0984704.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(29877..30125)
                     /locus_tag="KPG13_000964"
     CDS             complement(29877..30125)
                     /locus_tag="KPG13_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984705.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(30191..31207)
                     /locus_tag="KPG13_000965"
     CDS             complement(30191..31207)
                     /locus_tag="KPG13_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0984706.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(31265..32032)
                     /locus_tag="KPG13_000966"
     CDS             complement(31265..32032)
                     /locus_tag="KPG13_000966"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG0984707.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(32100..34289)
                     /locus_tag="KPG13_000967"
     CDS             complement(32100..34289)
                     /locus_tag="KPG13_000967"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG0984708.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        10962 a         6072 c         3682 g        13907 t
ORIGIN      
        1 atggtaaata aaaatacccc tatggcaaat aattacataa gggtattttt tatgtgtatt
       61 ataatcatat ctttgtgtat tataattata tttcatctga taattattta tatcatatta
      121 tcaattattc tttgaagcat ttgtagatag cttgccttat ttatatcctt agtatttatc
      181 aattccactt cgcctattac ttttttatct ttacatatct ttatttttcc taaaacagtt
      241 ccctttttaa ttggcatctt aggatttttt actatttcaa ttttcttctc aaattcttta
      301 gaacttcctt ttttaataag agcatttaaa tcttctttag caaccaattt aactttgttt
      361 tcatcagcct tatccaatgt aagtgttgct atattatcac ctttactaca taacttaaca
      421 ctttcataat tagcaaatcc ataatttaaa aggctagagg catcattaaa tctttctgga
      481 gaagtctctg ctcctaaagt tgcagcaact agatgagtat tacctctctt tgcagatgct
      541 gataaacagt attttgcttc ttgagtaaaa ccagttttaa caccagttgc tccttgataa
      601 tgctttataa gtttattagt atttgcaaga cctactgtaa cttgtttctt ccctactaca
      661 accttatcca tccatgtagt taagtattta cttatcattt cgtgttttaa tagttctcta
      721 gacattaaag ctatatcatg agcagatgta tagtggtttg caactggaag tccatttgta
      781 tttgcaaaat gagtatcatt catatttaac tcttttgctt tagcattcat tctatcaaca
      841 aatccttcaa cacttccagc taagtgttca gccatagcaa cacaagcatc atttgcagaa
      901 gcaacagcta taccttttaa taatgtatca accttttgag tttctcctgg ctctaggaat
      961 atttgactac cacccatact agatgcagtt tcacttactt gaacttcatc atcaagctta
     1021 atcttgcctg aatccaaagc ttctactata agtagcatag tcattacctt agttacacta
     1081 gcaggaggaa gctttttatg agcatccttt tcatataaaa tttgtccact accaacatcc
     1141 atcaaaattg ctgattttga agatatattt agttggttgg catcagcatt ttccttatta
     1201 tttgcaaaag aaaagcttaa aggagtaagc gcaattaata tagccaaagc agtactagct
     1261 aattttttca tcgatgtcac ctcttaaagt ttatttaaac ttattttctc acatttaact
     1321 ttttttattc taaatgccta gatatacctt gacctaaatt ttttttcttt tcaagctttt
     1381 taaagtaacc ttctaaaaca cctttattag acgcattttc tattatttca aaagcatctt
     1441 cataattcat tccatactct cctgctattt ccatttttgt ttgttttaaa gcttttatag
     1501 cattttgtct tgaaacatca tccataagaa tcctcctatc agtaaaattt atatataagt
     1561 agtttttact aataaaagat tgttataatt ggtattttat gtaatatata tttttttaaa
     1621 atgagtatta aaaagacctc ttgtaattct taagataata tcaattggat ttttgatatt
     1681 actttaaaaa ttacaagagg tctaattttc aaatttatag tttttttata ttatacttcc
     1741 attatgatag gaagtatcat tggatttcgt ttagtttttt gatataaata ttcttttagg
     1801 ttttctttaa tattattctt taaataagcc cattctttaa tatttttctc ttcacattca
     1861 tttagaacat tcttaattat atcctttgct ccatccatta aatcttctga ctctcttaca
     1921 tatacgaatc ctctggaaat tatatctgga cctgctaaga ccttaccctc atccttggaa
     1981 atagtaacta caactatcat aagaccatct tctgataaat gctttctatc ccttaaaact
     2041 atattaccta catcgcctac tcctagacca tcaactaata tgtttcctgt ttgtatacgg
     2101 tttgctacct tagcagaatt tttatctaat tctaaaacat ctcctgtttg catgacaaaa
     2161 atattttctt tatccatacc aagctgttca gctatttcag catgtctttt taacattcta
     2221 tattcaccat gagctggcat aaaaaacttt ggtctaacta atgcatgtat aagttttaat
     2281 tcttcttgac aggcatgtcc agaaacgtgt atatcagcta tatcactgta tactacttct
     2341 gctccttttt caaataagaa attgattact cttgatatta atttttcatt tcctggtatt
     2401 ggatgagcag atattataac taaatcgcca ctctttattt ctactttttt gtgctctgaa
     2461 aaagccattc tagcaagagc tgacataggt tcaccttgtg aaccagttgt tattatcact
     2521 aattcactat cttcatactt acatatatca tttaaatcta taagcatgtc atcagaaata
     2581 tctaggtatc ctaattcttt tgcaactcca actacattga ccattgagcg acctgatata
     2641 gcaacttttc tattaaactt ttcagcagta tttattattt gttgtaatct gtgtatgttg
     2701 gatgcaaagg tagcaactat aattcttctg ccatttcctt ttgcaaataa ttcgtcaaga
     2761 cctaccccaa cagtcttttc agacatagta aatcctggtt tttctgcatt agtactatct
     2821 gccagcatta aaagaactcc ttttttgctc aactcacaga ttctatgcat atccataaca
     2881 tctccatcaa taggagtcaa gtcaatctta aaatctcctg tgtggtatat tataccttgg
     2941 tctgtgtgta ttgcaataga gtaagcatct ggtatactat ggttattttt caaaaactct
     3001 acttccatat tatcaagctt tataacttgt ctaggaccta taacatttaa cttaacatta
     3061 ttcattttat gttctttaag ttttacttgt ataagaccta tactaagtct tgccccatat
     3121 acaggtacat ttattttctt taatatatat ggaagagctc ctatatggtc ttcatgtcca
     3181 tgtgtaataa atataccctt tattttatcc ctatttttta ctaaataagt tatatctggt
     3241 ataactatat ctactcctaa catttcatcc tctggaaaac taagtccagc atctataaca
     3301 attatttcat ctttatactc aacaacagtc atattttttc caacttcgtt aagtccaccc
     3361 aaggccatta cctttatctt gttggtgttc tttttgaaca attgcatcat ctccttctgt
     3421 ctatttaatt ttttaacacg ttctacaatc ataaataata tcaaatcacg cactcgctac
     3481 cagttattct tattgtacca taattcgtat catattttaa gctatattta aaatacattt
     3541 aacgtattat tttgacaaac ctattttatt ttgatatcct ctaaatatta tatgatttaa
     3601 tatcggataa tttaggagga tttatgcgtg gtaaaactca ttgtgcaata ggaatcttaa
     3661 cagctatcca aacatcttta atatttaaaa ttcctatatc tttagttgat attttagttt
     3721 cagctacttt tgctgtacta cccgatttgg ataaatctaa ttctatggtc tcaaatttca
     3781 ttctaaaaaa caatgtatcc aaatacatat atagaatttt tatatatgct gttaatatta
     3841 ttattttctt tatttcaata aatataaatg ataattttta tttaagcgca ataattacat
     3901 ttgttgcaat tataattatt gaggcaaaac taacccacac atttctaaga aaagtttttc
     3961 tatcattaat atttatacta ttagctatat gcttatacat catagaagtt gaaatatatt
     4021 ttacaatttt ttgtctaatg ttgtcaattt ttccctggtt aaagcacaga agtttttctc
     4081 atagcatatt tgcaactata atagtatatt ttttactaaa acaaatagaa ttaatcacta
     4141 atattaataa tttatctttt tatggtacga ttggttatgc aagtcatatg tttttaggag
     4201 atttatttac aaagcaagga ataccaattt tttatccgct aagcgaaaag aaaatttctt
     4261 taggtttttt aactgttgga ggaccattta gtaatttcat agaaaaatca ttcatttttg
     4321 tacttatagg attaattata ttttcaatat taaaactata agcatcttga aaactgtata
     4381 tttgcatttt atagatggtt tataataaaa aatggaagaa tagcatagaa tataaaagtt
     4441 ctatactatt cttccatcga cattaagcaa tagaagttta ttcttctgaa tcgcttactc
     4501 cattacactc gtcacatagt ccataaaatt taacatcatg atcaagtatt ttaaatttgt
     4561 acaatgattg tatctctttt tctatattgt ctaaaaggtc ttctttagct tccattatct
     4621 ttccacaatt tttacaaata agatgatgat gattatgaca gtcttcttta tgtagactaa
     4681 tctcatatct tatacatcca tcgtctaagt ttagttttga tattaatcct atttcatcca
     4741 atagctgcat tgttctatat acagtagcta agcctatttc aggacagtca accctaacta
     4801 aatcatagat ttcctcacta cttagatgtg aatggtcatg tttaagcaat atttcaacta
     4861 ttgctcttct ttgtggcgta atcttaaaac cagtttcttt taacttgtct tttaataaat
     4921 ccattgtatt tgccataaca tcacccttac tattttcaat tgataatctt tattaaataa
     4981 tattgtaatt gataattcat tttttgtcaa gacatttaaa taaatttaat ttattctcat
     5041 aatataaata tatacccata aaagatatat tattcaccaa aattcataaa ttttttggta
     5101 tttttaataa aaaacaaaaa aacagatata tactataaat gcttttacaa ttaataatgt
     5161 ttaattaaag caaatataat aaatatctga tttttatata caaacttaat tacttatcta
     5221 gttaagtcca tttgattcat cttcgtcgta atttaaacct tcttcttcca ttatagcagt
     5281 atacacagct acaacagttt catactcttc atcactttcc aaaggtatta agatttcacc
     5341 ttcttcatcc tcatcaattc taaagattaa agcttcttca gcttcttcat catctaaagg
     5401 cattaatatt gcatactctt ttccttctgc ttctaatgtt aatattattt caaattggct
     5461 ttcaacgcca ttttcgtcta ttaaatttat tatattttct tccataatgt cattcacctc
     5521 aaatttattt ttatttttat tatttatatt atatatcata agtattcaat tttatagttc
     5581 ttttatgaat ttattttaaa atctaaatat ccttgcaaaa ttattactgc tgccaacata
     5641 tctataacct tttttctttt ttgtctacta acatctgctt ctaataaaga cctctctgcc
     5701 gctactgtag aaagcctctc atcccaaaac tctacatcaa tacctgtttc tgcttttacc
     5761 aattcacaaa acttgattac tttttctcct tgtggaccta aagttccatt catatttttt
     5821 ggtaatcctg atacaatttt atttacttgt ttttctttta ttattgcttt tagctcttca
     5881 atatcttttt tcttgccaac tctttttatt gtttttacac cttgtgcagt taatcccatc
     5941 aaatcactca cagcaactcc aatagtttta tcaccaacat caagacccat tattcttcca
     6001 tctaacatag ttcctccttt atctatttat attctataga ttaaatacat attctgaacc
     6061 tctcataact aaggtcacag gcttactgct tcatataatt ttacataatc aatcataaaa
     6121 attgcaatat gattttttat gtaagcatcc agtttacatt aataatcata ttgcattttt
     6181 tatttaaatc ctatatgaac agtaaaaatt tttttagtta tttttttcaa atattatcat
     6241 atttatactt attattatta gtggtataag tatatatata gatgctgtag atgcagcaac
     6301 tattccagaa tcattaacta aaagtgtaat tatacaacca accatagatg caataaaccc
     6361 tttaaacaag attggaaaat tattcattat ctttctaaag tgtctagatg gtttaaatat
     6421 aagcactgca attactccaa ttcctacaag taaaatattt acccatacac ttgtctgtgc
     6481 tagttgcata ttcatttgaa tttttcttcc aaatgtttgt aatatctctc ctggtccatt
     6541 taggaaaatt ctctgtacaa acatacctaa atgagattca ctacctgaaa gtatatctaa
     6601 agctgcaaat gcaaaaacga caactacagc tgaaacagct aataatacag ctttcttaaa
     6661 atctatttta atgtcatata taagcattat aaacaacaaa tatgctacac attctgatat
     6721 agctccacca acatttgcac ccattgaagg atatgcacta gttataagta ttattaatga
     6781 aaaaattaca acaagtaatt tagatacctt cttataatgt aataatactg ctaagccaaa
     6841 tactgcactt gctatagtta caccttcata ttcatttcct acaccatagt acctagctcc
     6901 tatgacagca tcataactca ttatgttatt ttgcattaag tatgttccaa atacagaatc
     6961 tataactatg actaaaatag taagtgttgc aaaaaatccc atgtgcttta tatcattgtc
     7021 cttaaataat tttctaccta acaagtataa tattagcgta gttgcaaata ttcccaaaga
     7081 tattgaaatt ggagttttaa aattcatcaa aggagctaac ataaatgata gtggcattat
     7141 tatgcctaat tttataaact ccttaagtac tttgaataca cgctctttat gtggcatata
     7201 atttctaaag aaaatcgcaa acatggcaat tacccaagaa gcagatatta cgcctacaaa
     7261 gccatttata atagttgacc ttattgaaga tatagacact atcctttcat aaagtgattt
     7321 tagatagcta atattgtcct ctttatttac taaagtataa cttctaccta ccatctcttt
     7381 attttcaaga ccaaatgtac ctagtatatc tactccaaca tctaaattgg caacaattcc
     7441 agcctttctt gttgtggcag attccagtag acctttccct tctccactaa attttattat
     7501 tggagaaagt cttctcttat tcttataatc taggtcactt ggaaatgcac ttgctatata
     7561 aacagtgtca ttttctccaa ccatagaaaa tacttgttca agatatttat ttatattttt
     7621 tacaatattt ttcttcatta tatcataagt attatcattt agatttaatc tatacatatc
     7681 taatctatat gtatctccaa gttctacaaa tatagcatca ctatttttat aggatttttt
     7741 agtttcatct agtaatttat cataatctgt agatattcca aatggcattt tagaatcttt
     7801 cttattaata ttatcaatat tacctgaatc aatccttcca tattggtcca tagctactaa
     7861 acataagttt ctgtttttaa ctaattgatt attttcaatt atatcagcat ttccaattac
     7921 agatgtcttt aaaccatttt ctgataaagt ttttcctaat gaccctaaat ttgctccata
     7981 ttctccatac tcaagatttt catttataga tctattaatg cctaaatcat ttatttcttt
     8041 agctttttga cctgttgcga cttcaaaagc tttagcagta tcttggtcta ctttttgaaa
     8101 attgatatag tcttcagtag ttacactagc tcttgttcct gcacccatac ttgcataaga
     8161 ccttctatca tcagtacctt gatcccctct tatattcatg agagctacat agcctctctt
     8221 ttctatctca tttttcaaga ttggaatatt catcatactt ccaagattgg tcctattcat
     8281 atcaataaat atgaccttgc ctttttcttc agcaaaacca tagctagcag ttgatacaaa
     8341 aatcataatg actattgcaa acaacgacat aatctttttt ctcatttatt tgacctactt
     8401 tctattcaga tagtgcgtga ttacttcttc aagtatctca tctctttcaa attttttaat
     8461 tatagctctt gcatttttat gactagttat atagctagaa tcaccagaaa ggagataccc
     8521 tataatttga tttatagggt tatatccttt ttcaactaat gctttatata caaaatcaat
     8581 agtatctcct acactcatcc tatcttctgg tattccttca aatttcatag tgtagtctaa
     8641 atctttttcc aaactaaaaa cctccttgag tgaatgttat ttaacttgtg tcttaataac
     8701 ctcacttgca tagcttaaag cttggtcaac cttagataca tcactagctc ctgcttgtgc
     8761 catattaggt cttcctccac cttttcctcc agctattttt gcaacttctc taacaatatt
     8821 acctgaatgt attcccttat ctaatacatc tttagtggca gtaactacaa agttaacttt
     8881 atcatctact acatttgcaa gtactactac accagaacca agcttatctc ttagattatc
     8941 tgctgtttct cttagtgtat ccatatccat accttcaaat ttattagtta tcaaattaac
     9001 tccatttact tcaactttag aatcaaaaat agaatcaact gattgtaaac tcatcttagc
     9061 tttcatatca tgcaattctt tagataagtt tttattttct tctaataaag aacttattct
     9121 ttgaattaaa ttatcttctt tagattttaa gtttacacaa acttcagaga taattccatc
     9181 tctttctttt aggtattcat aaacagcttt tcctgttatt gcttctattc ttcttacacc
     9241 tgctgctact ccaccttcag ataatatttt gaacattcct acttgagaag tatttgtcaa
     9301 gtgagttcct ccacaaagtt ctgtagaata atctcccata gaaactactc ttacctcatc
     9361 accatatttt tcaccaaata gtgctgtagc tcctttttct ttagcttcct ttatattcat
     9421 aatatcacat tttatgtcta aagatgataa aataacatta tttacttttt cttctattac
     9481 ttttaattct tcatttgata tcgcttcaaa gtgagtaata tcaaatctaa gtctttctgg
     9541 agttactaaa gaaccagctt gattaacatg ttctcctaat acttctctta atgccttatg
     9601 aagtaaatgt gtagcactgt gatttcttgc agctgacatt ctagtttctc tatctacgag
     9661 tgtcttcact ttgtccccat tacttattct tcctgatttt attataccta tatgttttat
     9721 actattatta gcaccttttt tagtatttaa tacttctaaa actaagtttt cattagttat
     9781 caatccacaa tctccaactt gtcctccacc ttcaggatag aaagtagtgt tatctagtac
     9841 tatagatacc ttatcgcctt cttctgcact ttgaactagc tcatcatctt taactatagc
     9901 ttcaatagtt ccttcaccat aaatttcact atacccatta aatgtactgt caactgtaga
     9961 ttctaatttt gatagagggt cttccttcca actttctcca tccatatttc ctctagcatt
    10021 tctagctctt tctttttgct tttccatttc ttcattaaaa gcttcttcat ctacagatag
    10081 atgttcttct tctaatattt ctttagttaa gtctataggg aagccataag tatcatatag
    10141 tttaaaagct tcttgaccac ttaggacagt ctttccttca ttttttaata cttctatata
    10201 agagttcaat atttctgttc cttggtctat agtttcattg aatttttctt cttctattcc
    10261 aactactttt ttaatataac tttctttttc aactaaaact ggatatgctt tatcatttac
    10321 ttttataact tcatctatta gtttctgtaa aaataattct tttactccta ataacttacc
    10381 atgacgagca gcacgtctaa ggagtcttct aagaacatat cctcttcctt catttgatgg
    10441 aagcactcca tcactaacta agaatgttac agctcttata tggtcagtta ttattcttat
    10501 tgatatatca ttttttggat tctctccata ttttactcct gttaacttct caactgcttc
    10561 tagtatagat ttaattgtat caacttcaaa gatagtgtct acgccttgca taatacatcc
    10621 cattctttca agacccatac ctgtatctat attcttgttt tctaatagac tataatttcc
    10681 ttcttcatct ctatcaaatt gagtaaatac atggttccaa aattctaaat acctatcaca
    10741 atcacagcct ggtttacagt ctggattgtc acatccatat tcttctcctc tatcaaaata
    10801 tatttctgaa caaggaccac aaggacctgt acctatttcc cagaaattat catcttttcc
    10861 aagtctaacc attctttctt ctgggaaatt catttctttt gcccatatat cataagaatc
    10921 atcatcttct tcatatacag tcacccatat cttatcttct ggtatattta gccattctgt
    10981 aacaaattcc catccccatt ttattgactc tcttttaaag taatctccaa atgagaaatt
    11041 acccatcatt tcaaagaatg tagcatgtct tgcagttata cctacatttt ctatatctcc
    11101 tgttcttata catttttgag atgtacacat tctcacactt ggaggtactt ctacacctga
    11161 aaagtagttt ttcaaaggtg ccatacctga gtttataagt aacaaactct tatcattgtt
    11221 tggtactaaa gaataactat ttgctacata atgacctttt gactcaaaaa attctaaaaa
    11281 cttacttctt atttcattta atcccatctt ttccatatca tgttcctcct aataaatttt
    11341 attttttcgt ttaaataaaa aacctcgtcc ctataaataa atttataggg gcgaggacaa
    11401 tcgcggtacc accctaatta tcatacaaag tatgatatct tcggaaaacc ttagctataa
    11461 cgtaactacc gtcaatctct acttaaatct tctttataaa agataaattg ttcaagattg
    11521 ctactaaaag gctgcttcaa atatcatatt ctaagggctt ccagcatttc cctctctctg
    11581 taagacatca aatattttac tattccttat cactgtattt gttttttaat taatatacta
    11641 attatattac taaattttac aaaattaatc aatactatat tttcatatac aataaattta
    11701 ttttataaac tttatttact cttaatatac tttattgctt atttttttaa ttttttagat
    11761 gtttttataa acttttctcg attatacctc cacctactac aatatctcca tcatacatta
    11821 ctacagattg acctttagta atagctcttt gagcttcatc aaaaactatt ttaatagtat
    11881 cttctcctac tctatgaata gtagctggtg atggttttgc tgaatatctt attttggctt
    11941 gaactcttaa gggttcttct aacgtatcta ttgatataaa gttaacatct ttagcaataa
    12001 gttccttttt aaataaatct tcattgtctc caagaactat ggtattattt attggattta
    12061 tgtctattac aaacattggt tttccaaaag taattccaag cccttttctt tggcctatag
    12121 tataatataa tataccctta tgttttccaa gtatattgcc tttagtatct acaaaaaaac
    12181 cttcttcaat acgcttttta gaatgttttt ttacataatt tgcatagtca ttatctttaa
    12241 caaaacaaat ttcttgacta tcaggcttat tgtgtactgc catacccatt tccttagcaa
    12301 tttctcttac tctatctttt tcatagtcac ctattggcaa taaagtatgc tctagttgct
    12361 cttgtgtaag attataaagt gcataagttt ggtctttttt atctgtaaca gattttttta
    12421 gcaaatatct tcctgtactt tcatcctttt ctatctttgc atagtgacct gtagctacat
    12481 aatcacaacc tatttgtcta gccttcttat aaaaatcatc aaattttata tgtttattac
    12541 aagctataca gggattagga gtttttcctt ccaaatattc atctataaaa taatctatta
    12601 ctttttcttt aaacacttct ctaaaattta aaacataaaa tggtatacct attttatttg
    12661 ctacccttct agcatcttca acagcagata aagagcaaca accaccttca atttcaacaa
    12721 catcatcatc ttgccacaac ttcatagtaa caccaatgac atcatatcct tgttgtttta
    12781 aaagatatgc agctacagag ctatcaactc ctccactcat acctatcatt acttttttat
    12841 tcataaacaa atttcacctc atcttttgca ttacttatta ttataccata tagagcaagt
    12901 attgtactta tttaaaatat agcttgttct attttctaaa aaaataatca tctttattat
    12961 ttgaatctta tggattattt tgttaactaa ttaacctcta aatcaagtta aaatacttaa
    13021 tttaggcaat aaaaaatagc tattttaaaa acagctattt tttatgtcct aattatatca
    13081 ttagtacttc tttttaatca ttgtaaactt ctcataacac aatcacttat ttttattctt
    13141 cttcttcaac atcatggtca tgagcatcat ctataacata tccatccaat tctggtatat
    13201 ggatattgtt cttttgagca taatctataa gagctgcttt tacagcttgt tcagctaaaa
    13261 ctgaacaatg catctttact ggtggtaaac catccaaagc ttctgcaaca gctttatttg
    13321 taagttctaa tgcatctttt atagtttttc cttttatcat ttcagttgcc atagaagaac
    13381 ttgctatagc tgaaccacag ccaaatgttt taaacttaac atcctttatt acatctccat
    13441 ctatgtctaa aaatatcttc attatatctc cacatgtagg gtttccaact tcacctacac
    13501 cacttgcatt atcaatttct cccatatttc ttggattcat aaaatgttcc ataactttat
    13561 cactatattg cattatcttt tcctcctact caatttatgt ctatttttta tgctttatct
    13621 ttatgtctat tttactaaac cttgtttctt cgcatcatca taaagtggtg acatgcttct
    13681 aagtctttct attacttttg gtaagttctc tagtatataa tctatatcat catctgttgt
    13741 aaaatctcca actgtaagtc ttaatgaacc atgtgctatt tcatgtggta aacctattgc
    13801 aagaagtaca tgtgaagggt ctaatgaacc agaagtacat gcagaaccac ttgaaccagc
    13861 aattcctaac atatctaata ataaaagtat tccttcacct tctataaatt ggaatgcaaa
    13921 gtttgcattt cctggcaatc tattttctaa gctaccattt actcttgtgt aaggtattct
    13981 ttctaacaca ccatctatta acttatttct aagacgagtt aaagtttcta catgattttg
    14041 catattttcc ttagctaatt ctgctgcttt tccatatcca actattgcag gtatattttc
    14101 tgtaccagct cttttgcttt tttcttgtgc tccaccatgg acaaagttat gaagtcttac
    14161 accttttctt atgtataaag ctcctacacc ttttggtcca tatattttat gtgaagacat
    14221 actcatcaaa tctattccta attctttaac atctactgga atattacctg tagcttgtac
    14281 tgcatcagtg tggaataata tcttatgttt tttagctatt tcggatattt ctttaatagg
    14341 ctgtatagtt cctatttcat tatttgcaaa cattatactt ataagtatag tagtatcttt
    14401 tattgaatct tctagttgct ttaagtctac ttttccttcg ctatcaacat ctaaataagt
    14461 tatttcaaat ccatgatgtt ttgctaagta ttcacatgta tgaagtattg catggtgctc
    14521 tatttttgaa gttattatat gattaccttt gtttttatta gcataagcta caccctctag
    14581 tgtccagtta tcactttctg aaccacctgc tgtaaaatat atttcactag gttcagcatt
    14641 tataagggct gctacttgtt ctcttgcttt atccaaagca tcttttgctt ctcttccgaa
    14701 tgtgtgaaaa ctagatgcat taccaaaata atctgttagg tatggcatca ttgcatccaa
    14761 tacttctttt ttaataggtg ttgttgcaga ataatccata tacaatcttc ttttttccat
    14821 tacattcatc tccaaatcta ttttttaata tttgttatat cattttctaa tttatttttg
    14881 ttatagtcat ttatcatatc tttaagagta attgaatcta taacatcttc tatacccttt
    14941 ttcatctttt cccaaactat ttttgttaca cacatgtttg agttttcaca tacatcttca
    15001 tctagtacac agtcagataa tgcaactggt ccttctaata ctacaagtat atctcctact
    15061 gttatgtctt cagcattctt tgaaagcaaa taacctcctt gagcacctct cacacttttt
    15121 actagacccg atttttttaa agatgaaaat atttgttcta agtactgatc tgatattttt
    15181 tgcttcttag caataaattt taatgatact ggtccattgt cttgatttaa tgctaattca
    15241 aacattgctt ttagtccata tctaccttta gtagacaact tcatatatta tcacctctat
    15301 atagaaatct aattcatact gttttagtag gaattacttt caagataatt ttattatacc
    15361 ctactaattt tgtcaagatt aattttaatt ttttattttg aataaattcc ccattttcca
    15421 atgttttcaa atcctagttt tttatatatc tttccagcct cttcattatc ataaaacaaa
    15481 caaggaattt tgttttctct cagtaactct gaacatagtt taattaggca ttttgtagca
    15541 agtcctttag ctctatattt aggatgtgtt cctactccaa ttatcattgc atgagtccta
    15601 ttttctgaag tactctttgc cattgacacc acctgtttgt taatctctat acaatatcct
    15661 ctacctgtcc ttagtccatt ttttaaattc tctaaagttg tactgtgaaa ttcatctatt
    15721 aagtcataaa gttttactgt cttttttaga tttccaagcc taattctctt aacctttgca
    15781 ttacaattat tatctatcaa aaacttttta gtttgaagtt tgcaaaaatc aactaccttt
    15841 aaattattaa gtcctaatct ctttgctaaa gcatctacag cacatatttt cccagatatt
    15901 tcttcgtaat tagtattacg aagtagatta taaaaacctt caacatcata ctctccatca
    15961 gaataaaaca tcataaattc aaaatattta agtaatattg cctttatttc accatgttca
    16021 cccacatctg cccatatatt taaaaaatta tttccatatc catatctttc aatatcccct
    16081 attataaaca aattgtactc aggttctttt tttaagtatt tcataactct actgtgataa
    16141 gttgagttaa gttttataat cataatatct acccccgata cataaagttc agtctaaaaa
    16201 tcactattta gatattctaa atattttttt atatttttct cagttttatt ttcttgtggt
    16261 atgtaataaa tactatcttt tatgctatct ggtaaatact gttgctttac ataatgattt
    16321 ttaaaattat gaggatattt gtatccacaa cccctaccta tttctttaga accagaatag
    16381 tgagcatcct ttatactatt tggtatatcc ttaacaaact ctttatccag ttcatccata
    16441 gctttcatta ttgctataca tgcagaatta gactttggag atgtagccaa aagaatagta
    16501 gcttctgcta atggtatttt cgcttctgga aatccaagtt gcatagctga gtctacacat
    16561 gcctttacaa ttacaatagc atttggataa gctaaaccta tatcttcact agctattact
    16621 aaaagtctcc tacatatact tatcaaatcc ccacctttta taagtcttgc tagatagtgt
    16681 atagatgcct gtgggtcact tcctcttata gatttttgaa atgcacttaa aatatcataa
    16741 tgactatcac catttttgtc atagtttata attttattaa atgtagatgc ccttattaca
    16801 tctgaatcta tataagttat attatcctta tttggtttag ttgaatagac taccacttcc
    16861 aaaatattta aggctcttct catatctcca tcagaaagaa ttgctatatc ttcaatagca
    16921 tcattattac actcaatatc catatagcta tcttcattta atacttcaac agctcttttc
    16981 agtccttttt ctatatcaag tttttctaaa ggtttaaatt caaatacagt agacctactt
    17041 aaaagagctt tatatacata atgatatgga ttttctgtag tgctagcaat caaagttata
    17101 ctcccatttt ccataaattc taatattgat tgttgctgtt ttttattaaa actttgtatt
    17161 tcatctatat ataataggac tccatttaca ttatttatac ttccaagctc tgatatcacc
    17221 ttttttatat cttctaaaga tgaattagta gcatttattt tatagaagct tttattgctt
    17281 ctttctgcta ttatttccgc tactgtagtt ttaccaacac caggaggacc aaaaaatatc
    17341 atatttggta aaaaattaga ttgtaatatc ttagacaata tttttccatt acctattata
    17401 tgactttgac ctattacatc attcattgtt tttggtctta ttttatctgc taaaggcatt
    17461 ttctcactcc tttttaatat acttatcatt accttaataa ttcgtctaaa aaaggtatct
    17521 atttgaaata gatacctttt ctaatctaaa cctataaaat atagattatt gattgttttt
    17581 taattttttt aattcatctg taagtttgtc attaagtatt cttatatgag tacctttcat
    17641 acctaatgat cttgattcta tcacacctgc actctcaaat tttctaagtg catttactat
    17701 tacagaccta gttataccaa ctctatcagc tatcttactt gctacaagta aaccttcttt
    17761 tccatccaat tcagcaaaaa tatgttcaac tgcttcaagc tcggagtagg acagagtgcc
    17821 tattgccatt tgaactacag ctttctttct catttcttct tccaattcct cacctattgc
    17881 tcttaatatt tcaagaccaa caacagttgc actgtattca gctattacta aatcatcatc
    17941 attgaatgaa tttgaatatc ttgaaagtac caatgttcct aatctttgac cgcttcctaa
    18001 tattggaact actgtagtat atttttgtaa tcttccatgt tcttcaggaa agatttctaa
    18061 tatcttctca ccatttaaat tttctaatgt ttcatcaatt tttaacacat tttgagtata
    18121 ttcatctgaa aatttctttt gcttagtata ctcatcttct ataactgaac tgtcttgaac
    18181 atcatttaaa tgaagaccta gtactttacc ttttgcactt actacataaa cattagaact
    18241 taaaacgtcg cccaatgctc cggccagtaa atcaaaagag acactgcttc caccacttgt
    18301 ttgtaatgtt ttatttattt tccttgtttt ttgtaacact tcacttgcca tttaatcatc
    18361 tcctcatgaa atttaggcct tttaataacc ttttatattt tctaatatta ttttcacatt
    18421 taatgaaaat ttatttttct attagttgtt tacacttgaa agattatcat agattggaac
    18481 ctttttcaag agaattatgt gatttttttc aattttcact cttttccgtc attatgctct
    18541 tttgtctctt tattacattc tttattagaa cacactgttt ttgtttcaga tttagtcact
    18601 ttttcaacca tatatgaacc acacttctca caaaactctc ctgttggctt attccaagct
    18661 acaaagtcac agtttggata attagaacac ccataaaaag ctttacctct tttagatttt
    18721 ctaagtataa tatctccttc ttcacatttt ggacatttaa caccaatttt attcataatt
    18781 ggtctagttg ttttacaatc gggatagttt ttacaagcca taaactttcc aaatctacca
    18841 tatttgataa ccatattaga accacagttc tcgcaaatct catcagtttc ttcatccata
    18901 tttacttttt ctatattttc aagagcttct tcaatagcct ctttaagtgg tgcatatgat
    18961 tctgatacaa tctttttcca atctgtattg ccttcttcta taacatcaag tttactttcc
    19021 atatcagcag taaaatctac atctataaat ttatggaaat tattttccaa aatatcagta
    19081 actattattc caagttctgt tggacataga cttgttcctt gcttttcaac atactctcta
    19141 ttcagtatag tagctattgt tggagcatac gtacttggtc tacctatacc tagttcttct
    19201 agtgttttta ctaaactagc ttctgtatat cttgctggtg gttgtgtaaa atgttggtct
    19261 ggcaatatat tttctatatt taatatatct cctttttcta tacttggtaa aattttgtct
    19321 tctctttctg aaaaattata taccttggta taaccatcaa atttcatttt tgaaccagtt
    19381 gccttaaata caatatcgcc tattttacat tctacattta gtatatcaaa cactgaatct
    19441 tccatttgac tagctacaaa tcttctccaa attaaattgt ataacttgta ctggtctttg
    19501 ctcaatgagg cttttatact ctcaggagtt ctatcaactg aggttggtct tattgcctca
    19561 tgggcatctt gaaccttttt agtatccttt ttttcttttt tatcaccaat gtttttatag
    19621 tagttttctc ctaaattgtt tacgatataa cccttagctt tttcttttgc ttcatcagat
    19681 attcttttag agtcagttct tatatatgat ataagaccaa cagtaccttc accttctatg
    19741 tcgatacctt catataattc ttgtgctatc atcattgttt ttttagtagt aaaaaataac
    19801 ttatttgctc cttcttgttg aagcatactt gttgtaaatg gttttggagc tgatttacgt
    19861 cttgatttag attctatctt ctcaaccttt aaatctttac cttccatttc ttttagtatt
    19921 tcatttacag cttcttcatt ttctaattca atcttttcat catttttacc ataaaatttc
    19981 aaagttatat ctttgtcttt tactgtctta gcctcaacat ctattgtcca gtattctttt
    20041 ggaataaatg cctttatttc tttttctcta tcacaaatta actttgttgt aacagattgc
    20101 actctacctg cactaagacc ttttctaacc ttttgccata atataggact tatttgataa
    20161 cctaaaagtc tgtctagcac tcttcttgcc tgttgtgcat caaccaaatt tatatcaata
    20221 tttctaggat gtttaattgc ttttttaatt gcatccttag ttatctcatt aaactctatt
    20281 ctacaatcct cagattcatc taaatttagt atatgagcta aatgccatga tatagcttca
    20341 ccttctctat cagggtcagt tgctagatat acttgtttag attttttagc ttcttttttt
    20401 aattctttta ttacatcgcc ttttcctctt atatttatat attgaggttc aaaattattt
    20461 tctatatcta cacctaattt acttttaggt aaatctctga catgaccaac tgatgcttta
    20521 actgtgtaat gacttttccc taaaaatttt tctatagttt tagcttttgc aggcgattct
    20581 acgatgacta atgttttggc cataaaacca tacaccccca ctacctaatc ttaatttata
    20641 catttaaact gtacgtttta ttattcattt ctactactaa ctcatttagt actaattcat
    20701 tgattattga atttacatac tttatttcta taccagtata atcacaaata ctatctatat
    20761 gcaagtttcc ttctcttttt atagcttcaa ttatttgctt agacttggca tttagactta
    20821 tattatcata tttttgattt atttttttat caataatatt aaatatgtta tattcattta
    20881 aaatatcatc tatattttca attaattttg caccttcttt tataatttta tgacaacctc
    20941 tactcatttg agaatttata ttaccaggta ttgcaaaaac attttttcca tgttcaagag
    21001 caaaatctac tgtgattagt gcaccacttt ttattgctgc ttctacaaca actactccat
    21061 cacttaaacc actgataatt ctatttctat tagaaaaatg gtacggggct actgtcgagt
    21121 taatattata ctctgataat aataatcccc cattttctaa tattttattt gataaatgta
    21181 aattttgttt tggaagagga ttgtctactc ctgaaccaag tactgctata gttttagatt
    21241 ttccactcat acaacctatg tgcgagtatg catctattcc tatagctaat ccacttacta
    21301 tattaactcc atattgggat agttgacaac ttattgtttt tgcacaatta attccatagg
    21361 ctgtaggttt tcttgaacct actatagcta tattaaaatt attattaact aagcctatgt
    21421 cacctttgta gaataataac tttggagcgt tgtatatatt ctttaaattt tcaggatatt
    21481 ctttatcata tttacaaata tatttaatcg cttttttgta aagtagttct tttatatttt
    21541 ctaaataagc gtgacctcta tattttacta tattttttct aatattcaag cttatatttt
    21601 ttaaattata tatttctttt tcagaaaaat caaatatatc ttcaatattt actatttcat
    21661 tttctattat ttcaattgtt tttgttgtta ttccaccaat tgattttaac cataaataag
    21721 tatctctttt ttccataaaa tccacctcaa aaaactattt aaaataacta taataagcct
    21781 ttctaaatga aaaagcttct ataatacact gtgaatttat caaatctctt tcttctaaat
    21841 ctgctattgt tcttgccatt tttaaaagtt ttgtataact tctactgctt aggttgtatt
    21901 tactaaatat catctcagcc gtttttgaag cttctttttc cagtttacaa tatttaaata
    21961 aattgtaagc tttaatttca ctattatttt ttatattatc tttcttaaat ctatttatct
    22021 gtatttttct agcattttct actcttctct ttattttttg agagctttct tcttgtttta
    22081 aagaattaaa atctgaatat tttatagaat taacttctac aaaaatatca aacctatcta
    22141 acaatggacc tgatatttta tttatgtatc tatcaatttc gttacttcta catctacact
    22201 ctgtctctga catataataa ccacatggac atggattcat agctgcaact aacagtactc
    22261 tacaaggata ttttgcactg tactttactc tggatatatt tacatatcca tcttctatag
    22321 gttgtcttaa agtctcaagt atttttctat caaattcggc tatctcatct aaaaataaga
    22381 tacctctatg tgctaaagca atttctccag gtcttgcatc cattcctcct ccaattaaag
    22441 attgctttgt agttgtatga tgaggtgctc taaaaggtct tttatcaatt attcctttag
    22501 tttcatttat catacccaat atactataga ccttacttac ttctatcatt tcttctacac
    22561 ttatatctgg taaaattgtc cttactcttt ttgcaatcat agtttttcct gagcctggag
    22621 gtccaatcat aaacatatta tgatttccag cagcagcaat ttctgcactc ctctttacaa
    22681 aataatttcc ttttacatct ttaaagtctt catcatattt actttcttca ttattaatct
    22741 tagtatattt gcaatctata taactattat ctttttctaa ttcttcgttc tcttttcttg
    22801 acttatcatc caaaaaagac attatcctta ctttatcgac ttttatctct tcgtttaaaa
    22861 aatcgacaca ttcttttaaa cttttaatag gaattatatc aatttcatct acaaatagac
    22921 tttcttttat gttctcaatt ggtataaaaa tacgttttat gttctgagtt tttgcaccca
    22981 taattatggg caatatacct cttacttttc ttattttccc atctaatgat aattctccta
    23041 taaacatact ttctcttatg taattttcat ctttttttat taactctctt aaaaggccta
    23101 tacttataga taaatctaaa aaagctcctt ctttttttat atctgctggt gataaattta
    23161 caactattct cgtgcttgga aatttatatc cactattaag aattgctgac tttactcttt
    23221 ctcttgcttc tttaatttct ttgcctggaa gaccaacaat attaaaacta ggtattccat
    23281 tgcttacatc tacttcaacc ttaactaaaa agctgtcgat tcctactaag ttgcttgaat
    23341 taataatact taacatattt aatcctttcc aaaaacatta tcttttttaa aaagcattca
    23401 ttatgtgatt tattcttttt tctaataaat atacctctat aacatcaaat cttatgggag
    23461 tgttatttaa gttatttttt ataatataat aatttgcaac tccaattatc ttttttcttt
    23521 ttttgaagct tacagattca caagggtagc caaactttat gttagacctt gatttcacct
    23581 ccacaaatat aatttcatct tccatttttg ctattatatc tatttcccct atctttaatc
    23641 tgtaattttt ttctagtatc tttgctcctt ttgatagcaa ataattaact gctacttttt
    23701 ctccaaaatc acctttttct ttattattca ttattcactc caatacatta aattttcact
    23761 tttaaattat ggtcagtata caattttact attcaagttt ttggattgat tttatttcca
    23821 tcaatattaa taaatacttc tattgacttt attcttttat gatattattc tagttttcat
    23881 aggaaaagtt tatttttttg gaggagtgcc tttaatgaac agagaaaaat tagttgcttt
    23941 tatacgatat ttatctttat ttatattagt tgtaagctat ttgaaaaatg ttgaaaatac
    24001 attgtatccg gtcataacta tgataatatt atttctaatt ataataaata atcaagttag
    24061 atttttttct ttatcaaatg agaataaatt tatacttatt tcttactttt tagaacttat
    24121 attaattttt atactttgta attatacaaa aacttttaat tcgatttact ttgttccttt
    24181 aatattagat atatcatttt taataaagga aaaatataag tacatattgt tctcttttgt
    24241 tataatatct tctcttatta ttagtttaga taaaaatata tatttggcac tggagtcaag
    24301 ctctatattg ctaattataa ctgtactttc tatatatata gaaaatgaga atttatcaaa
    24361 attatacagt caaaatcttt atgataagct tagaatttct aaagatgaat taaaaaaagt
    24421 taatgctgat ttggaaactt atgcaagttc tatagaagaa ttagcaattt taaaagaaag
    24481 aaatcgaata tcaagagaaa tacatgatag tgtaggacat tctctttcta caacaataat
    24541 acaattaaat gctatagaaa aactcttaaa agacaaacct ctaatttatg acttagtaca
    24601 tgaacttaga gaatttgtaa ctgaaagttt tcaagatgta agaagggcta tatctgagtt
    24661 aaaacctgtt gaatatgaaa attaccaaag tttatttaaa atcaaagaat tagttaaaag
    24721 ctttattaag cttacaaata ttaatgttaa attaactatt tcaaaaaata cttggaactt
    24781 atccagaaat caatctatag ctttatatag acttatacag gaatcccttt ccaactcatc
    24841 gatgcatgga aaggcaactg aaatcagaat ttttattaca ttcaatactt caaatcttat
    24901 aattactatt agtgataatg gtataggatg tggcaatata aaaaaaggaa atggacttaa
    24961 tagtatcaat gagagaatat atgaattaaa tggtaaagtt gaatttgata attctaataa
    25021 tggtttcaca attagagctt cttttcctaa attttcaggg ggtgattttt ttgaataaaa
    25081 ttaaagttct tatagtagat gacgaaaaat taattagaaa aggtcttaaa attatattat
    25141 catcttataa tgatttagaa attgtaggag atgcttctaa tggatatgaa gctttagaat
    25201 tttgtaaaat gaatgatgta gacatagttc ttatggatat acgaatgaaa gtatgtgatg
    25261 gggttctagg tacaaggctt ataaaggaat ataataattc tataacatta ttaattttaa
    25321 caacttttaa tgatgatgaa tatataaaag atgctatgaa gtttggtgct tcaggatacc
    25381 ttttaaaaga tagctctgat gaagtattgc atgaagggat tcgttcttca ttttttggca
    25441 atattgtgct tgataaaagt gtagctgaaa aaatcatgac ctctgaaaaa acgattaaac
    25501 aagagcatct ttatgacatg tacaatttaa cagaaaaaga aatatctata attagactaa
    25561 ttgcaaatgg acttaataat aaagaaataa gtcaagaact attcttatct gaaggtacaa
    25621 taaagaataa cataactaat attttagcta aactagaact tcgtgacaga actcaactag
    25681 caatctttgc tttcaaaaat aaaattgtaa tagaataaat ctaaaaatat aaagtgactt
    25741 aagtcacttt ttttagtgac ttatagtaat agaattataa ttcacaattc tttattataa
    25801 tattatagct ttttaactta tacaaaatta ttttggaggg attactatgg atatagtcag
    25861 agtaaataat ataacaaaaa gatttaatga taaattagta cttgataaca tcagcttttc
    25921 tgttaaaaaa ggtgagatat ttggacttat tggcccaaat ggtgcaggta aatctacact
    25981 tataaatata attacaaatc ttatgttacc aaatagtggt agtattcaaa tcaatggttt
    26041 agatttatca aaagattata taaaagcaaa gtctataata gggttagttc cacaagaatt
    26101 agcaattata gaaactctta ccccttttga taacttagag tactttggag ctttctacgg
    26161 tttaaaggga aaactactta aagaaagaat cattgaagca ttagaagtta caggtcttac
    26221 tgaagtcaaa aagaaaaagg tcaaaaaatt atctggtggt atgcaaagga gattaaatat
    26281 tggaatagca ctcttaaatc atcctaaaat attaatattg gatgaaccaa ctgtaggtgt
    26341 agacccccaa tcaagaaatc atattttcaa ttttataaaa gatatatcta aaaaacatga
    26401 aactactgta atatatactt cccattatat ggaagaagtt gagcaccttt gttctaaaat
    26461 ttttattatg gatgaaggta aagaaattgc ttttggaaat aatgattatt taaaatcact
    26521 cgtatctaca aatacaaaat taattatgga aataaaaaat ataaatgccc aacttatctt
    26581 tgatttaaaa aatactaaag gtgttatatc tgttttagaa aataattcat tattagaatt
    26641 ggatatagat aaaaaattac aacttacaga tatactctct gtcattgaca aaaacaattc
    26701 aaaaataatg aaaatttctt atgaagaacc tagcctagaa gacgtatttt taaatctaac
    26761 aggaaaaaat ttaagggact aatggaggtg attttatgaa attatacatg agtgtaaaaa
    26821 ctatgctaaa gagtttaaaa agctccttta tacttaattt aatatatttt ttagctttgc
    26881 ctctgatttt atcttggttt cttgggatgg ttacagaaag catgtttcaa aaccctataa
    26941 aaacagaatc tacccccatt gttatatatg acaaagataa cacaagatta tcaaatgatt
    27001 tgacaaagta tttgaaaaat gatttatctt atatattaac agttaaaaaa gatgattcta
    27061 aggcagaatt aaagcttact atacctaaag gctatgagag tagtttatta aatgaaaaat
    27121 caaatactct aaacatagaa aaacttggaa ctcgtgatga tattgcaata ttgcttcaag
    27181 atatattaga tacttaccat gaaaaatttt atttaaataa ttctcagaaa ataagttcag
    27241 aagatttttc taaattattt aataaaaatt ctattgatac ttctataata aataacaatg
    27301 ttaaacagag ctcttatgaa tactttgctt tagtttcact aggatttctt gtaataattt
    27361 ttataatgaa caatattttg tcaaattata taagtgaatc aaagggactt tcaaaaagac
    27421 tttattctat gcctataact agagtacaat tcttaattta tgactttgta ggtttatgga
    27481 tttattcatt tatattctta ttattatatg ttttattctt tagaatactt ggaataacat
    27541 ttaaaggtaa ttttgccata ttgctcctac tttgtgcttt atcatcttat tttatgactt
    27601 caatatctac ctttgtaacc agctttttta gtaaaaaata cggtacagca atagtttatg
    27661 cattactctt tttacagaca atttttggag gtatcttcag tatgataagt gatacattta
    27721 ctaagcttac aagtttatct cctacctatt taataggaga attgtttagt aattatgaaa
    27781 catttaaaac catagattca ataggaaatt taattttcac atgtttaatt atctctacaa
    27841 tattaattgc acttgctatt gtaaaggaaa aatataaatg gagggaggtt taataatgat
    27901 tagattaact attgcaaata taaaaagata tctaaaaaat catactcttt taattaatat
    27961 ggttatgctt cctattatac taattttttc acttaatttt tttatcaaca actcgggaaa
    28021 tcaatcaatg tacttttctc cagtagcaat tgtatctgac tcttcaggaa agtatgaaca
    28081 taagttaata aatagttcta aattaaaaga aaattcattt agattaaatg aacaagataa
    28141 ggctatgaat ctattaaaaa ataataaggt ttctgctgta ttcgttttag ataaaaattt
    28201 ttcaaatagt atagataaat tacaacgacc tgtagttaaa tgctttaaaa ttgaaaatgg
    28261 tggtggtagt ctttgggcag aatcacaaat agaatctttt attactaaat cgttaaaatt
    28321 gaaagtggat aaaaatatag atgataaatt gacaaagaca aatataattg ataacaaacc
    28381 agataagaat aaaggttcat ttttagtagt cttcttgata tgctatttta tgtatattaa
    28441 tgcggcacat cttgcttctg atttatttac attaaaaaaa tcaaatgtgc taaaaagact
    28501 tatctctaca gataataaag atataaaaat aattttcagt atatttttag gactattttt
    28561 tattcaatca attgtataca cttttgctct attatgtttt tcaattatag aagactttaa
    28621 tttgagtctg aatgtattga tgattgtact agcaaatagt tttgtttcca ctggatttgt
    28681 attttgggta gctagagtat ttaaaaatga aagttctata agtcttatat ctacatttta
    28741 ttctttaata ggtctagcta tatctatctc tagtctaata ccatcaatgg ataaattatc
    28801 ctttatgact aatctttcaa agttcacacc attttattgg actatagatg ctattaaaaa
    28861 caatggtagt attttaataa atataatagc tctaatatta attggaatta tatttgtaac
    28921 tgcaggaagt cttaaactaa gagattttgc taaaaactaa taattattaa atataattag
    28981 tatctatttt tatataattg taaaagaata tttcaacata ttaaattatt ttatcttata
    29041 tttttctctt tagtttcaca catcaccaag tctaaactct atattatata ttaatatttt
    29101 tgacttgtca agttatttat gttataatat taatcattag ataattaaag cattgtacct
    29161 aagttgcaat atctaaaatt caatttggag gtataactag catatgtata aaattggtga
    29221 aagtgtaatg tatccaaaag agggagcctg ctctgtaaat gatattgtaa caaagaaaat
    29281 aaatcatgag atgcaaaaat actatgaatt aagtgtcatt tttaatagta accttaaaat
    29341 ttcgattcca gttctaaatg cagatagaat tggcatacgt ccagttatgg atggaaatga
    29401 tgttgataat tttatccaat ctattaataa aacagatggt gtatggattt ttgatagaaa
    29461 agaaagactt aaactgtacc aagataaatt tcattcagga gatgtttttg aaattgtaaa
    29521 attaatcaaa atgcttatga tacaagacag ttcaaaacaa ttgtgtagca cagataagga
    29581 gtttttaaat aaagctcaaa aatttgcttt atcagaatta gctgctgctc aatgtaaatc
    29641 ctatacaatg gtattagagg aaatgaaaaa acatatatta aattcaaaga atacaaatta
    29701 aaccagtttt ttaaactggt tttatttttt atactaatca tccatgctac tttagcttat
    29761 tttaaactgc ctaatctaat attaataaag ttatctcaat tagatagatg tattttattc
    29821 aaataaaaaa aactaagagt aaatcatatt actgatttac tcttagctaa taaaatttaa
    29881 tcttcttcaa gttctttttt ctcttgtaat aatacactta attcatttgc ttctaaaact
    29941 tgtgcctctg caatatcaag taactcttgt tgccattcca cacatttatc tttgtaatca
    30001 aaaggtagcg ttctgtcaac tatgtactta tcaatacagt cctccgcact tttaattatt
    30061 tcttttcttt catcaataaa tttttctaat tctactaagg cttcttcata agcttttcta
    30121 ctcatataaa ttcccccttt gtacaattat tctacattaa aatgattttt cctctttttt
    30181 gtataaaatt ttatttgtat ataacttttc catcaatatc ttttatagtt tgacatccag
    30241 ttaatatcat agaactactt agctctgatt gaagtttatt tacataagtt tctacaccat
    30301 cagttgcacc accaaaagaa gcagtcacaa aaggtcttcc tattaatact gcatctgcac
    30361 ctaaacctat catttttagt acgtctaaac cagttctcac tccaccatct gcaagtattg
    30421 taactttacc tttaacagca tcagcaattt ttggtaaaac ttcacatgct cctggtgtac
    30481 agtcaagcac tcttcctcca tgatttgata cgactattgc atccacacct gcttctactg
    30541 ccattaaagc atcttcaaca gtcattatac ctttcaatat aaatggtagt ttagtagact
    30601 ttactaactc ctttatttgt ttaacatttt taggaagtac tggttttcca tgtaatgata
    30661 aagttactag accacaggca tctatatcaa ctccaactgc aaatgcacca gcttcttcag
    30721 ctaatcttat cttttttata atattttcat tatcccaagg tttaataaat acaattccag
    30781 ctccattata ctttttgact acatctaaat tttccattaa aaaagcatct actgccgtat
    30841 caccaaccat tgcataaata ccactattaa cacatcctgc aactactggt tctatgtaat
    30901 ctctttcatt aatttttcca cccatattta aagtagttcc tgttactgga gctgcaaata
    30961 taggtgcact cattttcttt ccaaacatct ctatagatgt atcaggattt gatacatcat
    31021 gtattactct catatttact tttacttttt ctaaactctt gctattttct ataaaagaag
    31081 aaccacttcc tttacctccc atgccaggca cttcacctgc acaagcaacc ccattacaaa
    31141 ccttacaaac tttacaactt ccatttaaat tttctctagc actttttaat aattcattat
    31201 attccatctt taatccccca ttaactatag taatttatat tatatacatt atatcatatt
    31261 atatttacag tatatttttc aaaaaacttt ttctgtggat aggagttatg ccatattttt
    31321 caatagcttc atagtgttct ttagttccat atcctttgtg agatttaaac ccatattctg
    31381 ggtatactct atcatattgg tacattatac tatccctagt aactttagca agtatacttg
    31441 ctgctgctat tgatatagat tttgaatctc ctttcacaat tggattttga gatatgtcaa
    31501 tacctggtat tgttgctgca tcaactaata aataatccgg tgcttttttt aaacaattta
    31561 tagccttttt cattgccata tatgtagcat ttaatatatt aaattcatct atttcttcat
    31621 tgtttactat acctattcca taatctagtg cttcttcttt tattacttca aaaagttcat
    31681 ctctttttgc ctcactcagt ttcttagagt catttacacc ttctattttg gtatcttttt
    31741 taaatactac aacagaagct acaacaggtc cagcaagtgg tcctcttccc gcctcatcaa
    31801 ttcctccaat atataaataa cctttatcat agccttcgtt ctcaaatata tttatagttt
    31861 ccaatctttc ttcttctttt ctaatattat ccagcttttt tgccagtttt atagctagac
    31921 cttgtacaga ttttctttca tcaactctta acagctctat atattccata tatttttcta
    31981 cttctaaagt ctctattatt tctttaatct ccctcacact tttatcctgc ataccattcc
    32041 ctcatctttc gtttttaaaa atttaatttt taaatatatt aaaatttttt aattagagtt
    32101 taattgcatc cactatagcc acattttaaa catatactac atccacctgt atgtgcaatt
    32161 ccttcacttc cacactcagg gcacacaaat ttttcatctc cttcaaaatt tttatttatt
    32221 atttctttgg ctttttcatc tttattatct ttttcttttt tattattttt ataatattct
    32281 tttttactat tttctttttt atcttcttta ttattttctt cctttttttc atttattaaa
    32341 attccactcc ttttacatcc atctctaaat attgttactc ctttaagacc agcctcccat
    32401 gccttaatat atatattgaa aacatcttca actgtagttt caaaaggtat atttattgta
    32461 gatgatatag aagcatcaat atacttttgc catacttttt gcatgtttat tctttcttca
    32521 tatcctatat tcatagcagt aacaaaaaaa tctggtaaac attcttcatc tgtaatacct
    32581 ttcttgtcca tatactcttt aacaattgga gtatatactt tataatacac atcctcatca
    32641 tgcaagcttt cagtttttct tatatatgaa agattaaata taggttctat tccaccactg
    32701 atacctatca ttgtagatat ggaacctgta ggaggtatgg ttaaaagttg tgaatttcta
    32761 agcccatact ttttaaccat ttccttagtg tcttcattta gattttctat aaaaaactta
    32821 gatttagaaa tattttcata tttatactca ctaaatgttc catattcttt agcaattaat
    32881 gctgattgct tcacagcttc atttaacata gtcttaccaa ttaaatcaca tagttctaaa
    32941 gattcatcag aaccatatct tattcccatt tttataagca tatcagctat acccattaca
    33001 cctaaaccta tctgtctata cttatgtaca ctatctcttt gttcttgtaa tggatgcaag
    33061 ttcaatccct catctaagac atcatttaaa gctactacag cttctctaac gcaagatttt
    33121 aatttattaa tatcaaaagt agcatgttca ccaaatggtt gtattataaa ctctgacaag
    33181 tttattgagc caagtagaca acttcctcca cttggaagtg gttcttctgc acaaggattt
    33241 actcctgaaa agcaaaagtt cttatcttca ctcataaaat tatatgtttt tattttatcc
    33301 caaaacaata tacctggttc tgcataatcc cagttatttt tcacaaattt attaaataaa
    33361 tcataagcat ttactatctt aactattttt tctccagtag tttcaacttc aaaagtacaa
    33421 ttatataact ttttttctct tacagcttgc ataaattcat ctgttactct tacagaaata
    33481 tttgctttag taaccttctc caaatctgtt tttatatcta taaagtcttc tatctctgga
    33541 tgatttacat ccatagaaat cataagagct cctcttctcc ctctttgacc aattaagcca
    33601 gttgtcatac tgtaaagttc cataaaagag actgctccag tcgtatgttt tgcagcattg
    33661 ttaactttcg ctccttttgg tcttagcttt gaaatatcaa ttccaactcc tccaccataa
    33721 ctaaaagttc tagcaagttt ttttgcagta tcaaatatat cttctatgct atcttttggt
    33781 ggttctatta cataacaatt agaatatgtt actttttttc cctctttctg taatcctcta
    33841 tttgctaaaa ttcttccagc aaatagaaat tttttttgtc ttattagttt tgatattctt
    33901 gaactattat ttgaaattct ctttatccat tcatcaaaac tctcaccatt atgtctatac
    33961 ttgttcttcc atattgattt ttgtaattca tcaaactccc aagctttagt ccttatttca
    34021 gagcgtcttt ctctatataa tatgtagtgt tttgctatat cttttctatt tgattccata
    34081 agtagtactt caactatatc ttgtatttct tctacggata agcatttttc catagaatta
    34141 tagtcattag ctatttttct tgatatacca tatgccaact ctctatctac tccaccttca
    34201 ctatttatac tagctttata gacagcatct tcaatcttac ttttactaaa aagttctttt
    34261 gtaccatctc tttttattat gtatttcatt tttaaacctc caataaatta gtattaaatt
    34321 aatacacaca ttaactataa ctctaaacaa actttttata tatttgtata ttttcatatt
    34381 aattatctaa cataatatat attatactat tttatattca atataacact ccttatagtt
    34441 ttcttaactt gtaaaatatc ttcctaaaaa actcaaaaat aatataaaaa agtatataaa
    34501 agtcaaatta tactcttata tactttatct ttttatattt ttttcatttt aactttgatt
    34561 aattaataag tatttagaaa ataaattctt tattttttct ttacaaaaat atgtattgat
    34621 gtt
//
LOCUS       DAELTA010000023        33826 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.023, whole genome shotgun sequence.
ACCESSION   DAELTA010000023 DAELTA010000000
VERSION     DAELTA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.023"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            31..1143
                     /locus_tag="KPG13_000968"
     CDS             31..1143
                     /locus_tag="KPG13_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG0984709.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="KPG13_000969"
     CDS             1397..3421
                     /locus_tag="KPG13_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0984710.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="KPG13_000970"
     CDS             3641..4327
                     /locus_tag="KPG13_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG0984711.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="KPG13_000971"
     CDS             4422..5477
                     /locus_tag="KPG13_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG0984712.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="KPG13_000972"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="KPG13_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0984713.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="KPG13_000973"
     CDS             7194..8900
                     /locus_tag="KPG13_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG0984714.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KPG13_000974"
     CDS             8922..9995
                     /locus_tag="KPG13_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG0984715.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KPG13_000975"
     CDS             10007..10729
                     /locus_tag="KPG13_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0984716.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KPG13_000976"
     CDS             10745..11902
                     /locus_tag="KPG13_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG0984717.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KPG13_000977"
     CDS             11905..12744
                     /locus_tag="KPG13_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0984718.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KPG13_000978"
     CDS             12759..13934
                     /locus_tag="KPG13_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG0984719.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KPG13_000979"
     CDS             13963..14712
                     /locus_tag="KPG13_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0984720.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KPG13_000980"
     CDS             14734..15642
                     /locus_tag="KPG13_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0984721.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KPG13_000981"
     CDS             15690..16442
                     /locus_tag="KPG13_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0984722.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16396..17763
                     /locus_tag="KPG13_000982"
     CDS             16396..17763
                     /locus_tag="KPG13_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG0984723.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17769..18860
                     /locus_tag="KPG13_000983"
     CDS             17769..18860
                     /locus_tag="KPG13_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0984724.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KPG13_000984"
     CDS             18944..20857
                     /locus_tag="KPG13_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG0984725.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KPG13_000985"
     CDS             21056..21763
                     /locus_tag="KPG13_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0984726.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="KPG13_000986"
     CDS             22065..24176
                     /locus_tag="KPG13_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG0984727.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="KPG13_000987"
     CDS             complement(24374..25339)
                     /locus_tag="KPG13_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0984728.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="KPG13_000988"
     CDS             complement(25624..26580)
                     /locus_tag="KPG13_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG0984729.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="KPG13_000989"
     CDS             26948..27736
                     /locus_tag="KPG13_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0984730.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="KPG13_000990"
     CDS             complement(27859..28329)
                     /locus_tag="KPG13_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG0984731.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="KPG13_000991"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="KPG13_000991"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG0984732.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="KPG13_000992"
     CDS             29155..30060
                     /locus_tag="KPG13_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0984733.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30375..31922
                     /locus_tag="KPG13_000993"
     CDS             30375..31922
                     /locus_tag="KPG13_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0984734.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31925..32323
                     /locus_tag="KPG13_000994"
     CDS             31925..32323
                     /locus_tag="KPG13_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG0984735.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32363..>33826
                     /locus_tag="KPG13_000995"
     CDS             32363..>33826
                     /locus_tag="KPG13_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0984736.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKF"
BASE COUNT        13487 a         3591 c         5965 g        10783 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatttt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta tagaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagtaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggttcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaggaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa taggatatgg tggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaag ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaacta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagcgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa tccagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aataatccac aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaattg cttagaaaat ataaaaataa
    19981 attagattta aaaacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatt attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtacttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat ataaatgggg agcaactgga ccaaattctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca cgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggatgattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttga aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatga
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatatata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagcatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggtcagta cttattatgc aaagtttaaa attgtagaag atttgacgaa
    27361 agtagaagga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 tataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgcc ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatagat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaaatcctt tgcatcaaag atttttgatt cctttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggactaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatggttg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agacttgata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actaatcaac aacaaaatgt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttgcaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaattttaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaaatagca tgacaagaat ttacagttta aataaaataa
    30421 tttttattat aattggaatt ttaggaatag ttttactcac aagacatatt gaggtagata
    30481 ttaaagtttt aattttaatt ttattaacct tgtttagtat aaatgtattt atttcatatc
    30541 ttaaaattaa gttgtatgag gataaaatta atttaggatt gataaataat tcagataagt
    30601 ttataaatat tactaaagat gagaacttcc taaaacatgt tcaaaattat attatcagta
    30661 atgaaaaaga aaactgtgta atggcttgtt ttgatttatg tagactcaaa cttataaatg
    30721 atatatatgg ttatgagcta ggggatgaag tgttaaataa tattttaagt aacttaaaaa
    30781 attattttgg aaaagaagct atttatggaa agttaaaaag tgatgttttt gtattaataa
    30841 taaagcttga aaatagggaa cagaagattc cttatttagt aaatcttatt aagaataaaa
    30901 ttgtgatact aagtcagatt gacagtttta aaatggatat aaatattgag gcttctattg
    30961 gaatttataa atttaacaat aatgaaattg atataaaaaa agctatagat aatgctgata
    31021 tggcaagact taaatcaaaa ggactaaagc acatagagta tgtagaattt gataatgcta
    31081 tggaagaaga aatacaatca attatgaaaa tagagagaga tttattcttg gctattaaaa
    31141 ataaagagtt tgtaattcac tatcaaccta aagttgattc aagtacaggt aacattatag
    31201 gttcagaggc attgataaga tggttgcacc catcattagg catggttgga ccaaataagt
    31261 ttatacctat agctgaaaaa aatggtctga taaacaatat tggtagatgg ctaattcaag
    31321 aagtttttat taccattaac aagtggattt cagaaggtat aaatgtctta ccagtctcaa
    31381 taaatctttc tagagtagaa ctatataaaa atgatttagt tgattttttt aaactgatgt
    31441 ttaatacata taatattcca aaagaactta tagaattaga aattacagaa acgacagcac
    31501 taagagatgt aaaatttata agtgaaaggc tttatgagat taaatcttta ggtatgaata
    31561 tatcattaga tgattttgga gtaggtaatt caaattttat caaccttaag gggataccac
    31621 tagatattat aaaaattgat aggtcactta tattggatat agttacaaat acaaaaacaa
    31681 agtttatggt gaaagcaatt gttaaccttt cacatgattt aaacgtaact gttatatgtg
    31741 aaggggtaga ggatatgcat caggtcaaag tcttaagtga actgggatgt aatttcatac
    31801 aaggttatgt attttttaaa cctttggatg agatgaatta taaaaaactt cttactgacg
    31861 gaagttatat aaatctgaaa gatactcttt tagataaatg catgacagtt gaggaggagt
    31921 aaggatgatt tcagaagatg tagattattc aataagaatc ataagagcta tatttaataa
    31981 tggagaagat aaaccaatag atgctaaaat gatattaaaa agtgaagata taccaatact
    32041 aacactatac agactgctta gaaagctttg tgcaaacaat attcttactt ttaacaaaga
    32101 agatggcaag acatacatgt taaatgtgtc attggataca ataagtctaa gaactattgt
    32161 agaagtaatt gatggaaaaa tatatataaa taaatgtacg agatatgtat ctaattgcag
    32221 taattgctta aaaggttgta aagtaaatcg tgaattgcta gaaatagaaa aagaaattta
    32281 taaactttta tcgagaaaat ctataagaca aatactagaa taaaaaattt tcagctatga
    32341 taataattgg aggttttcgg ttatggatga tatatctcaa gataattttc tactgtctaa
    32401 agagtatgag aatagcttag atgtagatac taaaaaagca agtggaatat attatactcc
    32461 taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata taattaaaaa
    32521 tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt tagaagtata
    32581 tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga agtatgatga
    32641 aaattattgg actgttgata acatacaccg tcatatatta aactattgta tatatggtgc
    32701 tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata aaaaggtagt
    32761 aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata gtcttaaaaa
    32821 gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg gtcataaaaa
    32881 acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt ataaggataa
    32941 agctgactta tatttttgtt tttataaaaa gattatagat attttaaagc aaggagggat
    33001 tggcagtgtt ataactccaa gatatttttt agaaagtcta tcaggtaaag atttaagaga
    33061 atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg caaatatatt
    33121 taaaaatata ggggtatcta gttgtattct aacttttgat aagaaaaaaa caaaagagac
    33181 atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat ttgaaacatt
    33241 ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga gattgctatc
    33301 tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga tacaagaaaa
    33361 atgtaaatat agcttggaag atatagcaat aagttttcaa ggaataatta cagggtgtga
    33421 taaagcgttt attttaagta aagatgatgt aaagttgaac cttgtagatg ataaattttt
    33481 aaaatgttgg ataaaaagta agaatataaa taaatatata gtagataaaa gtgaatatag
    33541 gctaatatac tcaaatgata tagataatga gaatacaaat aaaaggatac tagatgaaat
    33601 tatagggcta tataaaacaa aattagaaaa cagaagagaa tgtaaaagtg gaataaggaa
    33661 atggtatgaa cttcaatggg gtagagaaaa gcttttcttt gaaagaaaaa agataatgta
    33721 tccttataaa tcaaatgaaa atagatttgc tatagattat gataataatt tttcaagtgc
    33781 agatgtatat tcttttttta taaaagaaga gtatttagat aaattt
//
LOCUS       DAELTA010000024        33792 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.024, whole genome shotgun sequence.
ACCESSION   DAELTA010000024 DAELTA010000000
VERSION     DAELTA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.024"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            229..1077
                     /locus_tag="KPG13_000996"
     CDS             229..1077
                     /locus_tag="KPG13_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG0984737.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1141..3063
                     /locus_tag="KPG13_000997"
     CDS             1141..3063
                     /locus_tag="KPG13_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0984738.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3145..4008
                     /gene="aroE"
                     /locus_tag="KPG13_000998"
     CDS             3145..4008
                     /gene="aroE"
                     /locus_tag="KPG13_000998"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0984739.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4208..5425
                     /locus_tag="KPG13_000999"
     CDS             4208..5425
                     /locus_tag="KPG13_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0984740.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5661..7526
                     /locus_tag="KPG13_001000"
     CDS             5661..7526
                     /locus_tag="KPG13_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG0984741.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7704..9068)
                     /locus_tag="KPG13_001001"
     CDS             complement(7704..9068)
                     /locus_tag="KPG13_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0984742.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9247..10590)
                     /locus_tag="KPG13_001002"
     CDS             complement(9247..10590)
                     /locus_tag="KPG13_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0984743.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10663..10764)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11093..11746
                     /locus_tag="KPG13_001003"
     CDS             11093..11746
                     /locus_tag="KPG13_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0984744.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11887..13158
                     /gene="brnQ"
                     /locus_tag="KPG13_001004"
     CDS             11887..13158
                     /gene="brnQ"
                     /locus_tag="KPG13_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0984745.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13360..14547
                     /locus_tag="KPG13_001005"
     CDS             13360..14547
                     /locus_tag="KPG13_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG0984746.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(14777..16801)
                     /locus_tag="KPG13_001006"
     CDS             complement(14777..16801)
                     /locus_tag="KPG13_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984747.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            17191..17859
                     /locus_tag="KPG13_001007"
     CDS             17191..17859
                     /locus_tag="KPG13_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984748.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            17861..18346
                     /locus_tag="KPG13_001008"
     CDS             17861..18346
                     /locus_tag="KPG13_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG0984749.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            18407..19528
                     /locus_tag="KPG13_001009"
     CDS             18407..19528
                     /locus_tag="KPG13_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG0984750.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            19809..20999
                     /locus_tag="KPG13_001010"
     CDS             19809..20999
                     /locus_tag="KPG13_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0984751.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    21133..21388
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21447..22448
                     /locus_tag="KPG13_001011"
     CDS             21447..22448
                     /locus_tag="KPG13_001011"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG0984752.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            22552..22941
                     /locus_tag="KPG13_001012"
     CDS             22552..22941
                     /locus_tag="KPG13_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG0984753.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            23102..24310
                     /locus_tag="KPG13_001013"
     CDS             23102..24310
                     /locus_tag="KPG13_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG0984754.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(24396..25472)
                     /locus_tag="KPG13_001014"
     CDS             complement(24396..25472)
                     /locus_tag="KPG13_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0984755.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            25701..26228
                     /gene="hpt"
                     /locus_tag="KPG13_001015"
     CDS             25701..26228
                     /gene="hpt"
                     /locus_tag="KPG13_001015"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0984756.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            26317..27249
                     /locus_tag="KPG13_001016"
     CDS             26317..27249
                     /locus_tag="KPG13_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG0984757.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            27311..28537
                     /locus_tag="KPG13_001017"
     CDS             27311..28537
                     /locus_tag="KPG13_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0984758.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            28621..28842
                     /locus_tag="KPG13_001018"
     CDS             28621..28842
                     /locus_tag="KPG13_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0984759.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            29031..29201
                     /locus_tag="KPG13_001019"
     CDS             29031..29201
                     /locus_tag="KPG13_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984760.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(29294..29755)
                     /locus_tag="KPG13_001020"
     CDS             complement(29294..29755)
                     /locus_tag="KPG13_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984761.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            29939..30406
                     /locus_tag="KPG13_001021"
     CDS             29939..30406
                     /locus_tag="KPG13_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984762.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            30449..32212
                     /locus_tag="KPG13_001022"
     CDS             30449..32212
                     /locus_tag="KPG13_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG0984763.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            32220..32828
                     /locus_tag="KPG13_001023"
     CDS             32220..32828
                     /locus_tag="KPG13_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0984764.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            32900..33469
                     /locus_tag="KPG13_001024"
     CDS             32900..33469
                     /locus_tag="KPG13_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0984765.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT        12784 a         3785 c         5750 g        11473 t
ORIGIN      
        1 attatcttat aaataaaatc tataaacaaa atctatgaat taataggaaa ttatcaattt
       61 gttattagta aagtctcgtg atacaataaa tttataaaat ctagataaat agaaatcaaa
      121 aaatactttt atttttaagg taaaataata ctactagtag gataaataaa aatataaaat
      181 caaaaatact attaattata aagaaaataa agggggcaaa tttaagctat gaataatcaa
      241 aaaaatctta atattccaat tacaattagt tcttatacat tgggaacaga agtttcattc
      301 cgtgatagag ttcgtattgc aaaagaagct gggtttgatg gtattggatt gcgtgctgaa
      361 aattatattg atgcaaaaaa tgcaggtgtt acagatgaag aaatgattgc aattcttaat
      421 gaatatgata tgaaagttac agaagtagaa tatataactc aatggggaag agaagaagat
      481 agaacggaag ctcaaagaga aaaagagcaa aatgtttatc atatggctca cttatttaat
      541 gttaaacata taaattgtgg attattagag atgataccag aagaacaaat tataactgca
      601 ttgggagaat tatgtgacag agcaggtgaa cttattatag gtcttgagtt tatgccatat
      661 agtggtgtac cagatttggc tactgcatgg cgtgttgtga aaggatgtaa tcgtgaaaat
      721 gcaatgctta tattagatac ttggcactgg gctagagcaa agcagacagc agaggacctt
      781 gcacctgttc cagcagataa gatagtatca attcaagtgt gtgatgtttt agaaactcct
      841 tatgaaaaac ttagggatga atcactacat gaccgtttgg ctccaggtga aggatatgga
      901 aacacagagg aatttgtgaa aataataaaa gaacacaata tatcaccaag agtcataggg
      961 gcagaagtaa tatctgattc tctaatatct aaagggttat ccactgctgc tgaaactgta
     1021 tttaatgcaa ctaaaaaagt tttggataaa gcatggtctg aagtatctcc aaaataaact
     1081 tatataatat aaaaatatac agagtattat aatataaaac ttgaaaggaa ggaaaacctt
     1141 atgaagatta gttctatgtt tacacctata agaattggtt caatgacagt tccaaatcgt
     1201 tttgtagttc cccctatggg aaacaatttt gcaaacacag atggtacttt aagtgaaact
     1261 tctaaggctt attatcttga gcgtgcttta ggtggttttg gattaataac tatagaatct
     1321 agtgttgtag acaaaaaagc aaaaggaggt cctcgtaagc cttgtttata tgatgatagc
     1381 acaatagata gttttaaaaa tgtaatagat gcttgtcatg atgcaggttc gaaggtatct
     1441 atacaattac aacatgctgg ttctgaagga aatgaaaaag tagcaggaca tccattaaaa
     1501 gctgcttctg caatacctgc atcaaatggt cgtaatacac cactagcaat aacaacagaa
     1561 gaaatatatg aacttataga atcttatgga gatgctgcat tacgtgcaca aaaagctggt
     1621 gcagatgctg ttgaagttca ctgcgctcat ggatatctgg ttagtagttt tatatcacaa
     1681 agaacaaata agcgtgtaga tgagtttggt ggatgttttg aaaatagaat gagattacct
     1741 cgtcttatca ttgaaaatat acgtaaaaaa gtaggaaatt ctctagcaat attatgtcgt
     1801 ataaacagca ctgatgatgt tccaggagga attgatgtac acgatagttc agtaatagca
     1861 gcatatctag aagattgtgg aatagatgga cttcatgttt cacgtagtat acatatacat
     1921 gatgaatata tgtgggctcc tacgacttta catgctggat tcagtgcaga attagttact
     1981 gaaataaaaa aagctgttag tataccagta ataacagttg gacgttatac agagccacag
     2041 tttgctgagt taatggtcag acaaggaaga tgtgatttag tagcattcgg tagacaaagc
     2101 ttggctgacc cagaaatgcc taataaagct aaaaatggaa aactagacga aatgatacca
     2161 tgtattgcat gcttacaagg atgtgtacca aatatgttcc aaggcaaacc tatagcctgt
     2221 cttgcaaatc ctatattagg acatgaagca gagcttaagc cagctgaaat tagcaaagaa
     2281 gttcttgttg ttggtggagg tgttggaggt atgcttgcag catgggtatg tgctaaaaga
     2341 ggacataatg taactttagt tgaaaaatct gaagttcttg gtgggcaaat gcgtttagct
     2401 gcatatcctc cagggaaagg agatattact aacttagtta gaagttatat ttcaaaatgt
     2461 aatcaatatg gtgtaaaaat atgtactaat acagaagcta cagtagaact tataaaggag
     2521 aaatcacctg atgtagttat tatagctact ggagcaactc cacttgtact tcctatacca
     2581 ggaatcaatg attcaggatt aattcatgct gtagaccttc ttgatggtaa aaaatcttgt
     2641 gggaaaaaag tacttgttgt tggaggtgga atggttggat gtgaagtggc agccttcctt
     2701 ggagaacaag aacatgaggt tactgttatt gagcttagag aagaagttgg tgcagatgta
     2761 atttcagaac accgtaaatt ccttatgaat gattttgaaa attataatat acataccatt
     2821 actggtgcaa aagtttctaa attttatgat gatggtgtcg actatttact tacagatggc
     2881 acagagcatg atttaaaagg ttttgattca gtagtacttg ctatgggttc tcgtaattac
     2941 gacccattaa gtgaagttat aaaagaagtt gtaaaagaaa cttatattgt tggtgatgca
     3001 gtaaaagctc gtagggcatt agatgcaaca aaagaagctt ttgaagtagc attaaatata
     3061 taaataataa ataaattttt ctttgtaaat aactgaatag gttatttgca aagaaaatga
     3121 taatattgag aggagattat agttatggaa atttcaggta gaacaggatt atttgcatta
     3181 ataggtacac cggttggtca ctctaaatca ccagtgatgt ataattatag ttttaagaaa
     3241 ttagatttag attatagata tttagccttt gatatcacag tagataaggt aaaagaggca
     3301 cttttagcaa taaaaacttt taatataaaa ggagccaatg ttactatgcc atgtaaatct
     3361 gctgttacag aatatatgga tgaactttct ccagcagctc gtataattgg agcatgtaat
     3421 acaattgtaa atgacaatgg aaagttagtt ggtcatataa cagatggagt aggctatgtt
     3481 cgtaatttaa aagaaaatgg ggtagaagtt aaaggtaaaa agattacaat tatgggtgct
     3541 ggtggtgcag caactgctat acaagtacaa tgtgctttag atggagcaag agaaatatct
     3601 atctttaatc ctaaggatga tttttataaa agagcagagc aaacagttga gaatataaaa
     3661 aaagatgtac cagaatgtgt tgttaatcta tatgatttag aagatacaaa caaactttat
     3721 gaggagatac aatcaagtga tatattgaca aatgcaactc ttattggtat gaagccttat
     3781 gacaatgaaa caaatataaa agatactagt gttttaagaa aagatttagt agtaacagat
     3841 gtcgtttata atcctaagaa aacaaaaatg attgaagatg ctgaagctaa tggatgtaag
     3901 gctattggtg gattaggaat gttattatac caaggagcag aagcttttaa tctatatact
     3961 ggattagaaa tgcctgtaga agaagttaat gaattatgct ttaaatagat aagagtaata
     4021 tcttaaacaa tgaaaaaaca tacgtatttc aatattttta ataataaatt acaaattgtc
     4081 atagtgggtt ttgcctcata gtccagttaa atttgaagct ttaactgact atactttaaa
     4141 tccattatgg ggatgagttt attgtacaca aaaatgttaa taaattaaca agaatgggag
     4201 aaaaattatg aacaataaaa aatattatcc tactgccata gcgttgtatt tttcatattt
     4261 tttacttggt attggaattt caattttagg acaatacaaa ccagaatttt catcaatgtg
     4321 gggggctaaa acattatcag atggaacatt ggatgtaagt attgttttag ctgttattgc
     4381 tgcactagga ttagggagac ttatatctta tccatttgca ggacctattt cagataagta
     4441 tggaagaaaa gtaagtggtc ttataggaaa ctttctacat gcaattttct ttgttggaat
     4501 tgtattttca ccaaattttt atatagcata tgtatttgca ataattggag gggcagcaaa
     4561 ttcattctta gatacctgtg taactccttc ttgtatggag atatttgcta gtttaggaac
     4621 aatagcaaat atgtttacta aatttacaat agctttagca caatttttgc ttccttttat
     4681 aattggattt gttgcagcaa attcaatatc atttaaagtt atttttatag taacagcgat
     4741 tcttattgta gtagatgcct ttttgatagc tgtgttacca tttccaccag ctaataatgt
     4801 tattgataac aaaggcaaaa cagttaaatc agagaagatg aaatttacac caacaagtat
     4861 agctcttgtt tgtattggat ttacatgtac atctacattt gtattatggt taaattgtaa
     4921 tcaggaattg ggtaaattgt atgggatggc tgaccctaca aaaattcaat ccttttactc
     4981 aatgggagta atatgtgcag tattaataac ttctttactt attaaaaaat atattaaacc
     5041 aataagaata ttagttatat atcctataat agctttactt atgctattag ttgtatattt
     5101 tgttcaaact ccaactattt gtatgttagg gggatttgta ataggttatt cagcagcagg
     5161 aggtgttctt cagttaacaa cgtcaactgc aaatgaaatg tttccaacta ataaaggaaa
     5221 aataacatct atagttatga ttgcatctag tatagcaaac tatgttattt taaatatagc
     5281 aggtattatt acaaaatcag ggggagttaa tggaccaaaa tatgttgtat tgtttaatgt
     5341 ggcaataaca tttgttggaa ttttacttgc attgtttgta aacatgagat atgaaaaaga
     5401 aaaagtgtat gattatgatg tctaatatta gtataatata tgcatagaga agtctaaatc
     5461 taggatagat ttttattaaa agtaattaaa attaataagg atattattta cagttataag
     5521 atagggtatt tacataatta ataataagga tattactaga atttcaattt ctagtaatat
     5581 ttttttgcgt ataaatgtaa acttgatgaa ataagtagta taatcaatat aaattacatc
     5641 atgtaaagga ggataatttg gtggagaatg tttttaagcg attgcaggaa tttaatggtt
     5701 atgatggata caaagaatca tttgaaatga attacctatg tatatatgaa agtataccgt
     5761 taagagaaca agtagagctt gcaaataatc tagtagatga aattttaaat atgtataaaa
     5821 gtgaatctaa tgaaatttat ttattggagg gtagtaattc taagtcttta atttgttatt
     5881 tcgaaatttt tatgaaaaag ataaatactt tggtaaaaga aatgattatt gatgagaaat
     5941 ggttatataa attaactaaa gaattaatat ataagagtaa aaaagttgag tatgtaaaac
     6001 tagggttagt tttaagtgag aaatatctaa atgtagaaaa cttaagagaa gttgtagata
     6061 cattttctaa atctggagaa tacgtatttt atctgagtaa tactattaaa aaacttgaat
     6121 tttacaatac atatttattt aatttatcta aaaaggctac aggttcaata aaagtatttg
     6181 ctattgttaa tatggaaaat ttagattcta aaataaattc ctatctaatt gaagatggtt
     6241 ataaggatac aaagtacgaa cgattattga tgaactatat tatttcaatt gtagatttga
     6301 atgaatatct tgaaaaacga gatttagata aagaaaagat aaataattta gcacgtctta
     6361 tatgcaatta cttgctaagt gtagaattta aatatatagg taataaatta gaattagtga
     6421 atcgattttt acctactgta gtaaattatg ggactaactt tgaaagttta tattcaattt
     6481 tcctaatagc tataaacgtc ttaaaagatg aaaatataga gtgcaataag attgaatttg
     6541 aaaaagaaat aaatggtatc ttgctcagtg aaaaatggaa aaatatatat tttgaagcat
     6601 taagagatgc tagtggaaaa acagaagata ttataaaaat gtcagaaatt tatgatgtaa
     6661 acttatcttt tgatgacttg ttgccatatc taaatagaga tattagagat tttgaggtat
     6721 attggcatat atctaaaaag ggaacgacaa gttctagatt aaaattatta aatttttttg
     6781 aagagacgtt taaaatagat gatttaattg gaaaaatgaa agatatagaa aaagataaat
     6841 tgactcaaga atattatgat gatatgttat tttttattgt gcttaaagga agtaaatcac
     6901 tatatcctga aggaaagaat atatctttaa aaggaatatt tggaaatata aatgaagtaa
     6961 gaaaagaatc cataaatatc ttaaaaagat acagagagaa actatctctt gaagaattaa
     7021 aaattgttaa agaagcgtat gaaaaagaaa aaaatgttat attgaaggat gaattaagaa
     7081 gagtattata tgaaagtaat aatttaaaaa aagaatttgt aaatattgaa aaaataaaag
     7141 tagatgaaca tggtaaggat atctatttga cttcaatagc tgttgctggt tcaaggttta
     7201 gaaatagaga atatcttgaa aaagaattgg agaaaagtaa gatatactat ttaactagag
     7261 agaaagataa tctatatgat gaaaaagcta ttaaaatagt tggtgaaact ggatatgtga
     7321 taggctatgt cccaagaaaa gaaaactata ttttgagcaa tttattagat ggaggaaaac
     7381 tgctgtattg tagagtaact gaatataatt tatatgaaga ttgtatctac gcaaatgtgt
     7441 acttgagtta taaagatgta attgaaacag ttgaaaactc tttaaagatg gttttagata
     7501 agagtagaat aaaacttata aactaagacg tttatatatt gcttagatga tgtaatccta
     7561 actgattggt tattttttct ggaattatgg tgtattttat gagcaatgct aatgaatata
     7621 aaataaaaaa acaattgttt tgtatatatg atgtatataa atatatttat atattcatat
     7681 ttacaaaaca attgaaattt atgttatttt ataattttaa atttagatgg aattttaact
     7741 tctgctttat cttttatttt agaagcttgt tttttaactt tatttaataa ttcagattca
     7801 tctacaacta atacttttct ttccttcatc aaccatttac catcacaaat agtagagtct
     7861 acattttcac tagtcataga ataaactata tttccaattg gattatgcat tggtaaacaa
     7921 tgaattgtat ctggatttag aataatcata tcagcttttt ttcctacttc aagactacca
     7981 atttcttttt ctagtaaagc acattttgct ccatttatag tagccatttc taagacttct
     8041 tcagctggta cgacagttgg gtttaaagtt cttcctttat gtattagtga tgtaagatac
     8101 atgtccctca taaggtccat tctattattt gaaggagctc catcagtacc aattgaaaca
     8161 ggtattcctt tttctaacat ttcaggaatt gatgcaaacc ctagaaccac tttcatagct
     8221 gaacctgggt catgagaaac ttttacatca tgtaatctaa ataaatcaat ttccttatga
     8281 gtaagccaaa ctgtatgagc agctaaaaga tttggtccta atacacctag tttatcaagg
     8341 tattcaactg ttccaactcc attgttttgc tttacaaatg aaatttcatc agctatttca
     8401 gcaatatgca tatgtatacc tgtattgtat ttatcagcta gttctttagt tctaacaagt
     8461 aattcttcag ttgtattaaa aatagttctt aatgcaaacc atattttaat tctctcatta
     8521 gatgtattat taaacttatg gaataagtct aattgagtgt tgatttcctc atcagtagtt
     8581 ttttgccatg cctcaggaag accatctcct gtatccataa ctgatttagc taaacaggct
     8641 ctaagtccag ttttatcaac agcttcaacc atagcatcta catattgacc accagattct
     8701 aaaaatgtag tgacaccaga ttttatcaat tcaacacaac atgcagtaga tgaaatcaag
     8761 gaatcttcat aattgaagct actttcgtat ggccaaattc tttctctaag ccaagttaaa
     8821 agtacaacat catctgcaag accacgtcct aattgttgag ataagtgaac atgagtattg
     8881 actagccctg gcattagtat ttttccttta gcatcatata tctctgcatc tttatctaca
     8941 atattaatat caaacttacc aacctctttt ataatattat tttcaataag tatgcttcca
     9001 ttttcatata cctctctatc tgcattcata gtaattagat atgcattttt tataagtaat
     9061 tgtgacataa acaccactcc ttttctatat atttttatat ctatatatta tgataataaa
     9121 ttttctatac aaagttaata gcaaaatcaa tttacatata aagtgatttt gctatttata
     9181 aatttaaaat aaataagatt ttattctata taatttaaaa tattgaccta gaactattta
     9241 aaaatattaa cttagaacta agtttacaac tgcaagtaaa cctataatcc aaagtgtaat
     9301 actgatttta tcagtttctt tagctactac atgcattatt atatatgaaa taaatccaaa
     9361 gcataaacca atacttatac tataagttaa aggcatcaat aaaactgtaa caaaagcagg
     9421 tacagctatt ttgctatcag caaaatcaat ctgttttatg ctttcaaaca taaacacacc
     9481 aaccattaca agtgcaggag ctgcagcgta agtaggaact ataccaagta atggtgtaaa
     9541 tataagtgta gctaagaata atgctgctgt tactattgaa gcaagtcctg ttcttgcacc
     9601 tgctgcaata ccagaagcag attctccaaa tgttgtaaca gtactagttc ctaagaatga
     9661 gcctattaaa gtagaagaaa catctgcata taacattctt cctaaatctt ttatttcacc
     9721 attctcatct tcaagaccca tttgtttaca acaagtaata agaaatgcta atgaatcaaa
     9781 catgtctata aacatgaaag agaatatagg tccaattaat gagaatttca atgcacctac
     9841 aatatctaat ttaaaagcaa ctggtgctat actaggaggt gttgagatta ttgctgttgg
     9901 caactccaca aatccaaata taacagatat tattgttgca actactatac ttatcaatat
     9961 tccaccttta acttttttat attctagtat tgccattaag ataagagcaa aaagggctat
    10021 taaaactgtt ggtgtaaaat ctgcaagtga aactatagta tctggattag ctattataac
    10081 acccatattt tttaaaccaa caaaagcaag gaaaagacct atacctgctg aagatgctac
    10141 tgccaattct ctaggaattg cgtctgcaat tgtctttcga agacctgtta ctgcgagtgt
    10201 aaagaagaca catccagata gaaaaactac cccaagacct gtttgccatg ggattcctgc
    10261 acctataacc aaagagtatg tgaaaaatgc atttaatccc ataccaggag ctagagcaaa
    10321 tggtgcattt cctaaaatcg caaatataag tgtaccaatg gctgtagcta gtatggtaac
    10381 tgttataagg gcggctttat ccattccagc ttcagataat attgctggat ttacgaaaac
    10441 tatgtatgcc attgttaaga atgtagtaac tccacctatt acttctgttt taaggctaga
    10501 gccacgttcg ctaaatttaa acattttgtc taaaaagctt aatgatgaat tatttccacc
    10561 tggttgtaca ttgttagtgt ttggtaccat ttttacctcc aaaaaacttt aagatataaa
    10621 caaaaaaata tatattcata tagatagaat agaacatata ttcagagttg acattctatt
    10681 catagaaaac ccatttaagg tgagtttgta gaaactatta gaccgtatta ctaatattat
    10741 atgaacattt tctatttgtt tttctgttgt attatatcgt aatattgtat tatttacaat
    10801 aaatataata aaattattcg tttttttgtc gattttataa aaaattaagg ttatttattg
    10861 aaaaaaatta ctttttccaa taattgaata tgtatgagta taatgtcaat tattctaact
    10921 tgttttatac tattaatgga agtttattat aaatgagttt gtgatataat tataaaagta
    10981 ctgaatacat ggatatttcg taaaaacttt ttaatatatc aaatatgtat attaagttaa
    11041 tatattaaaa aaaataatat caaaaataat ataatttata gtggagggga aaatgactaa
    11101 aattattaaa gtgcaaaaac ctaaatttac aggagaatta gaaattattg aaaatcttga
    11161 agatatactt gaagatatat ttaatgatga aaaaatattt aataaaatta tagaaaatga
    11221 agttataagt gatttagatt ctgtaagagt tagctttgaa tcttgtatat ttagaaatgt
    11281 gtcttttgaa aattgtaatt taaaaaagat tgatttactt gatgtggtat ttgaaaactg
    11341 caatttatca aatacatttt ttgatggagg aagtatatac agagttgaat ttaaaaatag
    11401 taaattgata ggagttagat ttgatgattg tatacttaaa aacgttttgt tcaaggatgt
    11461 tttaggtaag tattctaact tttcattctc taaatttaag atagtttcta tcttagattc
    11521 aaattttgaa aattctgtat ttcaagaagt aaaaggtgac aatctagtat ttgagtatac
    11581 aaatttaaaa aaagcaaact ttaatggaac taaactttct ggcatagatt ttactactaa
    11641 taatattgag ggaattgaga ttggtataga tgatattaga ggagcaattt ttgatgtgtc
    11701 acaagcaatt gatttaacga aattgatggg aattatcata aaatgaaaaa aatattcata
    11761 aaataaattg ggaaaacgtt aacaatgtgt gaatttttac cagattgaca tttttctgaa
    11821 aataaagtaa gattatctta atattcaaaa tattttcaaa gggggtaatt tagtgtgagt
    11881 aagcagatga gagatatttt tatagttggt tttgctttat tttctatgtt ctttggtgca
    11941 ggaaatctta tgtttcctcc atttttaggg atggaatccg gcaaagactg gttaattcct
    12001 cttattggat ttgttgttgc agatgctggt atagcaatgc ttgttattat agcaacagca
    12061 agatgtaagg gaagcatgga tgatgtactt aggagagctg gtaaagggtt agcaagagta
    12121 atgagtgtag ctgctctagc atgtttagca cttcttgtaa taccaagaac ttgcgcaaca
    12181 acatatgaaa tgggtattat gcctatattt ggagaccatg gtccagtagc taaagctgta
    12241 ttttcaataa tcttctttgg gctaacttta gcatttacta ttaggtcttc taaggttata
    12301 gacataatag gtaagtattt aactccagcg ttattattag tattatttgt tttaatagta
    12361 aagggtataa tttctccagc aggacctatg agtcctgagc atatgataga taaaaatcta
    12421 tttggtgaag gtatatcaca aggttatcaa acaatggacg ctctaggtgc agcatctatg
    12481 gcaactataa tgttgatgtc tataatagct aaaggatata catcagataa agaccaaata
    12541 ggaatgacta ttaaagctgg ttttgtagcc tgtgtatttc tagcagtagt atatggtggt
    12601 cttacttatt taggtgcaac agtttcaact ttatatgata ctagtatacc tcaagcatct
    12661 ttattagttg aaataactag tatattgtta tatggtcaag taggaaaggt aattttaggt
    12721 gtaattgtgg cacttgcctg tctgactact tctacaggtt taacagcatc aatatcaact
    12781 tattttgaag gaataagtaa ggcgagttat aaacaactgg ttataggtat atgtgtagct
    12841 agtatgctta tatcaaattt aggtgttgat tctataatag ccatatcagt tccaatccta
    12901 caaactatat atcctgtttt attagcaata gttgttatgg agttggctgg taaacatata
    12961 aagaatgata atgcttttaa aggtgcagca tatgttacat tagtgattag tttattatca
    13021 gctataaatg gaatgacagg agctgttcca tttatacaaa gcttgccact tgcaggtttg
    13081 ggctttaact gggtaatacc agctatattg ggtggaatag ttggtaactt tataaaaagc
    13141 aacaaacaag tagcataata atttgtaatt aatttaattt tataatatat taatttttag
    13201 tactatgggt aggtttaatc taaacctaat ctacccatag tactttttag attggattat
    13261 aatagcatgt atataatttt ttataatata atttttcttt gaagtatgat aaaattagta
    13321 atagtgataa gaatgtacaa atttaggagg ttttaattaa tgcttagaaa aataacagca
    13381 tttatgctat ccatatgttt aattattgga cttacaggtt gtagtttact tgtaggcaag
    13441 aaagataaag agaaaattat agatgatttt aaagttgcag tggtaactca accattatct
    13501 gaaaataaag tacaatacaa tatggtggaa gagatggcaa aagaatatga ggaagagaat
    13561 aaaattgata aagacaaaga tggtcaaact aaagttaaac aaactataaa gcatgttgta
    13621 ttgccagaga attttacttc taatatagac agtgctatta ataagatagt aaagttggct
    13681 gacgataaag aagttcaggc tattgtagtt tcaactgacc aagcagggct tttacctgct
    13741 ttacaaaagg taaaggaaaa gcgtccagaa ataataacta tttctgcccc tatgggagat
    13801 gataaaaacc aattaagcca atttgttgat gtgaatctag gggttagtgc agaggaaaga
    13861 ggaaaagtat tagctgaacg ttcaaaagaa atgggagcaa aagcttttat tcattatgcc
    13921 tctacagatg atttgaaaga tgttaatata gccaaaagat tagaaatgat taaagagact
    13981 tgcaaaaata taggattgcc atttgtacaa gttaatactc caaatataaa tactgaagaa
    14041 gacaaaaaca aagttaagca gtttttaaat gaagatattg aaaaacaagt taaaaaatat
    14101 ggaaaagata taaatgtatt tggtgtaaat gagtatatgg atgaagttat attgactaag
    14161 gctttagaat taaagtatat agttgcagag caaagcaatc catcaccaat acaaacatat
    14221 cctagtgtta tgggtctaaa aatatctgaa aaagatgctc aaaattatga taaaataaac
    14281 gacatgataa gtgaaaaggc taaggctttt ggcatgtcaa atagacttgg tggatatcct
    14341 atgccaatgg atgcttttct accatcagta gcaatttatc ttgctactga aatggtgaaa
    14401 caagatttaa cacaagaaga tgtatgtgac ccagattatt tagaggcgtt tacagaatta
    14461 agatttggaa tcggttcaga atttactcct ttaacagagg tactttataa ttatcaatca
    14521 gtaattttaa gccaattaat ttattaatat taaggacgtg tctcaaaatg aactttttag
    14581 tttatgaggc actctttttc tgttaaatca aatatatttt tatcttttta acaattaaaa
    14641 agaagcttat ccatatgttg agatagcttc gtttttaata taaataagtt ttattatttt
    14701 ataaaaaatt tatagaaaaa taaaaaatta taattatata taaattttga taaagtaaag
    14761 atgaattact ataggtttaa attatattat tttctattat ttttatgcct ttctcagtta
    14821 ttgatgagcc ttttcttccc ttactagaaa ttaagtatcc attgcgtgtc aaatattcta
    14881 gcatatttct tacttcgtat tctgtgacat tatattccat aaatttcatt aactttgtcc
    14941 tccccattgg tttcatgtgc aatgatttta ggatagaaat gcatatgttt ttgtcatatt
    15001 ggtttaaatc attgaatatg gacatattac ttgacatatg attattattt aattttgaag
    15061 atatgttttg aggtaaatca tttattgtaa gataattgga acccatgtaa gccatatatt
    15121 caacacaatt tctcagttct ctaatattcc ccatccaagt gtatgaattt aaagcttcta
    15181 aaagactctt atcgagtttt atgtttttat agggaagttc ttttaaaaat gattctaaaa
    15241 gaagaggtat atcttctaat ctctttctaa gaggaggtaa atctaaagtg aacatactta
    15301 tcctataaaa taaatccatc ctaaatttat tttctataat taatttctct aaatctttat
    15361 ttgtggcagc taatattctc acatcaattg gaataatact tgtaccacca atttttctaa
    15421 tttctttttc ttgtaagact cttaaaagtt taacctgtaa atcatgtggc atatctccta
    15481 tctcatctaa aaatatagtg ccagtatgag ctaattcgaa taatcctttt tttccacctt
    15541 tttttgctcc agtaaaagaa ccatcttcat atccaaatag ttcactttca agtaattcac
    15601 ttggtaatga agcacaattt attgctacaa aaggtttatt ttttctattt gaatgattgt
    15661 gtattgactg agtaaaggct tctttacctg ttcctgtttc acctgttatt aatataggat
    15721 tatctgtaag agccattttt tttgcctttt taatacaatc ttttataata gaactatttc
    15781 caataatatt attaaattta tatttagcta cataacctct tttgacagaa tcacttctta
    15841 ttttttgttc taatatttct atattgttta catcttttaa tgtaattaag caaccctcta
    15901 tatttttata aagataaaat ggttttttag aaagtatgaa ttttttatct atttcgtcta
    15961 tgtatataat catattatct atatttaagt tctttgtaat tttatctaag aaactctttg
    16021 gcatatattc cattaaggaa ggtgatttta taagtttttc atctagttcc aacatattaa
    16081 gtaaagactt atttacaaaa gtaaccttat taaaggtatt gaaaaatatt agaccatcac
    16141 ctatttcatc aatggtcatg taaagtatag tttttaattt agatatatta tctaatgatg
    16201 tatttaagaa atcatgactt agtgtctcct tggacaattt gtataatttt tctataaatt
    16261 tctcattttt tagttttaat actactaata atgacatata tgtatctaat gagatttttc
    16321 tccaacctaa atcaacaata ttttttatgt attttggtat aaatctaatc tgacctggtg
    16381 ttatagctgt atctaaatca tatatttcat cacaatctgg atatgtcggt acaaaatcta
    16441 tatgatttat accaatttta tatagataag tagccatctc aaatgctgaa gttttattgg
    16501 ttgtcataag taacgatttt gtacctggag gcagtttcat cattttttca tatccaagtt
    16561 ttgaaagtgc tttcgttcca tgaataattt caatatcttc cttaaaatgt tttattaaaa
    16621 aattactagt taaaggtgat agagaaagca ctacatctcc ctctaaattt tcttttattc
    16681 catcaagtat attttgtgtt tctatgatta tttcgtctcc aaaaaagttt gttaattctt
    16741 ctttgtagtg gtcactattc aatttatcat atgttataag gattaacttc tttttatcca
    16801 ttttatttct cctaacaaaa tagtatttat ttttaaagta tttaaagtat aaggcaagtt
    16861 atattataca acaaatagta gtataaattt atatatttga ttaaataata ggttttttta
    16921 ggattttatc ctataaaaac ctattttgaa attatttttg caagataagg aaaatagctt
    16981 tttatattgt aatgtatatt ttaaataatt aataaaaaaa tattatattt ttaaacttga
    17041 aaagtaagta attataagct atttaatcac tggaataaat ttacaataaa aaaataaata
    17101 caaaggcttt atcattaagg tattggcata agttttgctt attaaacaag tatactaata
    17161 aaattcattt aaacaggagg gagaatttat atgagtaaat tagaatcaac aacagaaaaa
    17221 gttgaggtaa aatggtatgg gtatgtagca ttaatattgg gagcattatt tttttcagga
    17281 atatttaaag atgcaccagg accattaaaa gtattggatt ttaacaatgt attaggttct
    17341 tttggaacat tgggaacaat taatgatggt gtaggtacat tagctgcaaa ctttagaggt
    17401 gatggaggta ctggccctcg agatggatgg ctatatgcat tgacattaat accatcagtt
    17461 atgtttgcac ttggagtagt tagagttata gaccacttag atggaatgaa ggcagctcaa
    17521 aagcttctta gtccactatt aaagccatta ttagggttac ctggatttgc aggactaact
    17581 ttgatagcta gtttacagtc tacagatgca gctgcatcta tgactaaaga acttaaagat
    17641 gatgggtata tagatgaaaa acaaaaagct gtattttgtg catttcaatt ttcaggtgcg
    17701 tctgcaatta cgaatttctt tgcttctgga gcagcactat ttccatttat aggagatgta
    17761 ccaatattta taccacttgc tttaatatta attatgaagt ttgttggggc gaacttactt
    17821 agattatatt taaataaatt tgacaagggg gaagcttaac atgagtgagg tagcaaaaaa
    17881 agaagtaaaa gttaaaaaag gaaatgtgat agatgcgttt attggaggag ctagaaatgg
    17941 attccaaata agtacaaatt caatggctcc aaatgtcatc ttcggttttg ctataatttc
    18001 tgtgttcaac ttaacaggtc ttttggatgt aatagggaca atatttacac ctgtaatgag
    18061 tatctttgga cttccaggtg ttgcagcaac agctataatg acaatattta tatcaatggg
    18121 aggagcatct ggagtaatcg caggtttatt tacagcaggt caaataagtc aagcacatgt
    18181 tgcaattcta ttgccaatgt tattgctttc aggagcgcag atacaattta tggggagact
    18241 tttaggaaca gctgagttaa aaactaaata ttatccacat atgtttgtta tagcaacttt
    18301 aaatggttgt attgcaatgc ttataatgaa ttatttcgta gtataggtaa ttataataaa
    18361 aatttaatat aaatggaaag tttaaatttt aaggaggagc tagattatgg gaactaacaa
    18421 agattatatt gagtatgtac ataaagtgta tgattatcta cacactatac cagaaaaagg
    18481 atttgaagaa tataagacat cagagtttgt tttaaaagag cttgaaaaaa tggggtttga
    18541 aaatattaat aaaggagttg caggaactgg tattatagta actatagatt ctaaaaaaga
    18601 aggaccagta tttgctataa gagcagatat ggatgcattg gaatttatca tagatggaga
    18661 aaagaaaatg atacatgctt gtggtcatga tggtcatact tctatgcttt taacagctgc
    18721 aaagttaata aaagagagaa atttagtaaa aagaggtaag ttaaaaatac tttttcaacc
    18781 aggtgaagaa aaattatatg gagcattaag agtaattgat tctggtttgt tggatgatgt
    18841 agaagaaatg gtagggatgc atttaagacc tggacaagat tgtaggttgg gaaaggcgat
    18901 acctgcaatg taccattgtg ctagctgtat aatggaattt acgattaaag gtatcacttg
    18961 tcatggttct aaaccacatt taggagtaaa tgcagtagat gctgtctgtt tagcagtgaa
    19021 tgcaataaat gcaataagag taaatccagc tataccaaat tctataaaag taacaaatat
    19081 atcagttaag ggagaaactt ataataatat accagatgaa gcatttatgg ctttagatat
    19141 aagggcagaa agtaatgata taatagaaga attaaaagaa aaagcaatac atgctgtaaa
    19201 aactgttgta agttctataa atgcagaagc tgtactaatt tccaaagatg gagtaccagc
    19261 agcagaattt gatgaagaac ttgtaaactt ggctgaagaa actataaaag atgtattggg
    19321 agctgatgca tctataggta tatttaaaaa ttctggtgga gaagacttcc attattatac
    19381 acaaaaacta aaatgtaaaa ctacatatat tggattagga gctgatgcta caccaggatt
    19441 tcataatcca aatgtaacat ttgatacgaa agctttagaa tatggtgtag atatatggtg
    19501 tagattggtt gaaaagagat tgggataaca tccaaactaa taattctaca tttaaaatta
    19561 taattataga agttatagaa aaagattgct tatgttaatt agaaaataat taatatgagc
    19621 aatcttattt tgatataatg aaactgattt tggaatattg aaaaaatatg atggaaaagt
    19681 aattatattt taaatctatt ggacaaaaaa gtgatattat tataatatta agaattgtaa
    19741 aataatacca aagtgcatct agtaatagac ttaacaatta taaaaatttt attcttagga
    19801 gggaaaaaat gagtaagtat aattttgaca gagtaataga tagagtagga acagattgtg
    19861 taaaatggga ttttagaact aattgttcaa caaaagcaca aaaagatggt ttgccttttt
    19921 ggattgctga tatggatttt gaatgtgcag aaccaataat agaagcatta cgtaaaaaag
    19981 ttgaacataa aatatttgga tatagttcaa atgatagtga taaatacttt aatgcagttt
    20041 gtgattggta taaaaggaga tttaactggg aaataagtag aaaagatatt atttttagtc
    20101 ctggtgttgt acctgctgtt gctattttag taagaatatt aactaattct aatgaaggtg
    20161 taatagtcca aaaaccagtg tattacccat ttgaagctaa gataaagagc aataataggg
    20221 acgttgtaaa caatcctctg atatatgaga atggtactta taggatggat tatgatgatt
    20281 tagaagaaaa agctaagtgt agcaacaata aagtactaat actttgtagt cctcacaacc
    20341 ctgttggaag agtttggaga gaagatgaat taaaaaaggt tgtagaaata tgtaaaaaat
    20401 atgacctatg gataatagct gatgagatac attctgattt aattagaaaa ggatttaaac
    20461 atactccttt acagtcatta tgcccagagt acaaggataa aatagttaca tgtatagctc
    20521 caagtaaaac atttaatctg gcaggaatgc agttgtctaa tataatagtg aataatgatg
    20581 aactaaaaaa gaaatatcaa gaagaggtta cagctgttgg agttgcaact tcaccaaatc
    20641 cctttgctat tgtagctaca atagcagcat ataatgaaag tgaagactgg cttaatgaat
    20701 taaatgatta cttagataat aatatacaat ttattgatga atacttaaaa gaaaacctac
    20761 caaaagtaaa acttgtttat ccagaaggaa catatctagc atggttagat tttactgctt
    20821 atggattaaa tgaagtagaa cttgaagatt taatgtttaa aaaagctaat gtattatttg
    20881 atgaaggtta tatatttgga aaagaaggaa taggatttga aagaataaat gtagcttgtc
    20941 cacagtcttt attaaaagaa tgtatggaca gattaaagac tatatttgaa aatttataaa
    21001 gtataggtaa aaatttataa agtgtttaaa aataaaaaaa taataaactt gacaaatttt
    21061 aactaatcac ttatcatact attatgaata aatagaaaac ataagagagc taaattttat
    21121 tgaattaaac tcttaaagac tttgaaagga agagtagtta tagaatgtaa ttaaagcgag
    21181 ttggtgatgg tgtgagacca atatgaagtc tatagtgaag aacatcctgg agttgctaac
    21241 cgaaatttta aaagagtagg cttagtcgga accaaaccgt tatcatattg gggtgtatcg
    21301 agtaatatct gtacattgtg agctgggttt gaatttattt caaactaatc agggtggtac
    21361 cgcggaaaag caacctttcg tcccttttat atagggatga agggtttttt taatggaaaa
    21421 atatataaat atagggagtg aatgatatga gcacaataat accaaaggat tataaaagta
    21481 gtttgaacgt aattgataca caaatagcta taaaaaaatt aaaagatttt tttgaaatga
    21541 gactaagtaa tgaattaaat cttataagag tttcttctcc gttatttgta ttgccagaaa
    21601 caggtgcaaa tgataattta aatggagaaa aggctgtaag ctttgatata ccatttatga
    21661 ataaaaatgc agaaattgtt caatcacttg caaaatggaa aagattagcc cttaaaaaat
    21721 atgggtttga agttggtagt ggtttatata cagatatgaa tgcaataaga aaaaatgaag
    21781 aattagacaa tatacattct ctttatgtag accaatggga ttgggaattg gttatagata
    21841 aaagtagtag aaatgaaaag actctaaaag atgttgtaaa aagattgtat ggagtattta
    21901 aagatactga aatttttgta tgtagtatgt atgaaggtat aaaagaaata ttaccagaag
    21961 aaataacttt tataacatca caagagttag aagatatgta tccagaatta tcaccaaagg
    22021 aaagagaaga taaaattgta aaagagaaga aagcagtatt tataactcaa ataggaaaaa
    22081 cattaatatc aggagaaaaa catgatggaa gaagtcctga ttatgatgat tgggaattga
    22141 atggggatat attattttac aatcctgttt tagattcagc attagaatta tcttcaatgg
    22201 gaattagagt tgatgaagaa tctcttgata gacaattgaa acttgctgga tgtgaagaga
    22261 gaaaagagtt tgactatcat aagatgcttt taaatggcga acttccgtat acaattggtg
    22321 gaggaattgg tcagtcaaga atatgtatgt atttcttaca aaaagctcat ataggagaag
    22381 tacaagttgg agtatggcca caggatatga ttcaaaattg tagtagtgca ggaattgaat
    22441 tattataatt taaatataaa ctaattgata aaatttatat ctaatatgtt catcaaatta
    22501 attatatcgg ttataatgta gaataaccta taattataag aggtgataaa tatggagttt
    22561 ttaaaaataa tcattaatat agttttagat attttaaaaa aaattttggt cagatttaaa
    22621 aatgctaaat ttggtttggt ttttgtattt gatttactta aactacctga ttttatgaca
    22681 gataaaagaa tcaatatagt tgataaaata aaggtaattt ctgtattaat ttttactata
    22741 tcctactttg tatctggggt tgatataatt ccagagatga ttgcaggggc atttggtttt
    22801 atagatgatg ctatagtttt aatttggagt ataggtatag tgaatgaaga aattaataag
    22861 tatagagtta tcgcaaaaaa agataaacat tcaaatataa ttgaaaatgt agaattttct
    22921 ataaaagatg aagaagaata acaatttata tatgttattc ttttttttat taacaaaaaa
    22981 tggttaaatt gttgacggag aggtaaaact ctattatcat aataagtgga tatccaatat
    23041 ttaactaagt attcaatgtg caagaatgct taatttaaaa aagttatgga gggtaaatat
    23101 tatgaagaaa aaaataggtt tagtgcccaa actaatcatc ggtatcatag tcggtatttt
    23161 aatcggaagt tatgcaccag aaataatagt acaaatatta gtaacagtta gtactttatt
    23221 ctcagcattc ctaaaatttg ttataccatt tataattata ggttttgtaa cagcaggtat
    23281 agctgattta gcaacaggtg caggaaagtt attggggata actacgggaa tagcatatgg
    23341 ttctacctta atagcaggat tattatcatt tgcagtatct acattaatct tcccaaattt
    23401 tattgatgca agtgttgcaa gtcaaattgg agacccagag gcaggaatgt tagctccaat
    23461 attcacaatt ccattagagc ctatggtaga tgtaacagct gcaatcgtat ttgcatttgt
    23521 aatgggactt ggaatatcag ctttaagaaa tcatggaaaa ggagagactt tatttaattt
    23581 cttccaagaa tttcaaggaa tcgtaacgaa aacgttgtca acagtgatta tacctttatt
    23641 acctttatac atagcaggaa cttttgcaaa tataactata gcaggggaag tatggacaat
    23701 attaggggta ttctggaaag tatacttagt agtaatacct ttacattttg tatatatggc
    23761 atgtcaattc acagcagcag gtgtattttc aggaaaaaat ccagtaagaa tgttaaaaaa
    23821 tcaagtacct ggatatttaa cagctatagg aactcaatct tcagcagcaa ctataccagt
    23881 taatgttggt tgtgcagaaa aaaatggagt aagtaagcaa ataagagaat ttgttgtacc
    23941 tctttgtgca acaatacatt tatcaggtag tatgatatca ataacaggat ttacaactgc
    24001 agttttaatg atgaatggta tgccacatgg aataggtgta ttattccctt atattgcaat
    24061 gttaggtata gcaatggttg cagcaccagg agcaccaggt ggggctataa tgagtgcact
    24121 tcctttccta ccaatggttg gaataccatc agatggtggg cttgcaagtt taatgatagc
    24181 tttatactta actcaagaca gttttggtac agcatgtaat gtatcaggag ataatgcaat
    24241 agcagtagta gtagataaaa taaatgatgg aatgagtaaa aaaggaaata aagaagagca
    24301 aagtgcttag ttttttaata atcaaaaatg agttatctca aatagagata gctcattttt
    24361 tatggtataa aaaattataa aataaagtga tttttttatt taaaaattgc tttaagagat
    24421 gatattaata atttattttc attaggtttt aatatacata ctctaaagaa atactcatct
    24481 agaccttcaa atgaacaaca atctcttatt attatttttt gtggaagcag ttgctctcga
    24541 agagatttag cagtcaattc ctttgtcttg attttacaca atatgaaatt acctttagta
    24601 ttgtatatat ctaagctttt tatattctta agctctttaa gcaggtaatc tctttcagtg
    24661 ttcattaagg aaatagtatt tgatataaaa tctaaatcac taaacatgat ttcacccatt
    24721 ttagaagcta ttatgtttat attccataaa tctagatttt tatttatttc atttttgaca
    24781 ttagtgttag atataagacc atatccaagc cttattccag gcgttgaaaa aaattttgat
    24841 gtacctctta taacgaataa attagaataa tcatcaacta attgagtaca agagtatgta
    24901 tctgtatcag taaactctac ataagtctca tcaatcatta aaaatgagtc tgtatttttt
    24961 aaaatttctc tcacttctac tttagtaaat gcaaatccag ttggattact aggattacag
    25021 attactacca aatcataatc tttagcattg atagttttta taagattttc taaatttata
    25081 tgaaaattat cttcttcttt tgcaaagtat ttttctatag aacaattaat tttagataac
    25141 tctttttcat actctgaata ggctggagag agtaaaagag cctgtttagg gttaattgta
    25201 tgtataaatg atgaaattag ttctgtagct ccactgccaa gtactatatt atctatagaa
    25261 catttgcagt aattggatat agaagttttt aagtctatgt aatcagggtc aggatacata
    25321 gataccatat ctatattgtt tacaatgtat tgtttggcta aacttgatgt accaaaagga
    25381 tttatatttg agctaaaatc cataaattct tcctttgaaa agccatactt actagataaa
    25441 ctatataaat tagctccatg gtttgtgctc ataaaagtac ctccaaaaat aaaattatat
    25501 aatcgataaa aataactgat taatatatgt acatatagat tataatacaa aggaatcgtt
    25561 attccaaatt tttaatgtaa aaagcaaaaa agtatggata agctgttaat ttaggtttaa
    25621 aaatgatgaa aaagatttta attgatattt tggtatgcta taatatataa gttaatagaa
    25681 ggaaagcggg aggatacaaa atgtacaaag ttacaggaaa aatgttgaca gaagaacaaa
    25741 taaaagaaaa agtttatgaa ttaggaaaaa agatagaaga agactttaaa ggcgaagatt
    25801 tactagtggt aggtatactt aaaggagcta gtgtatttgt ttcagattta ataagatgta
    25861 tagatttgga tgtaaatata gattttatga gtgttacaag ttatggaaac tcaacagagt
    25921 cttctggaac agttaaaata ttaaaagatt tagatgtaga tattgaagga aagaatgtgt
    25981 tgattgtaga ggatataata gattctggtt taacattaag taatctagtt gcagctttaa
    26041 aaactagaaa tccaaaatct ttaaaactat gtactctact tgataaacct cagagaagaa
    26101 aagctaatat acctgtagac tatgttggat ttgttataga agacaaattt attgttggtt
    26161 atggaataga ctatgctgaa aagtatagaa atttacctta tataggtata gttgaggatg
    26221 tagaatagat ttaaatgtga atcaaaagca ctaatttcta agggagttag tgctttttag
    26281 ttaattatat aaaattttag taaaggagac atctatatgg atattcagtt aaatatatta
    26341 ttatttttcc aaaatttaag aaatccaatt ttgaacttta tatttcttgt ctttacaata
    26401 agcacagaag caccgttatt aatattaatt acaacaatta tatactggtg tataaataaa
    26461 aaatgtggac agaaaatact gtttgctata attggaaatt ttgttgtaaa tttaggcatc
    26521 aaagagtttg taaaagctcc cagaccaata ggaataaaag gtcttgaatc attgagagtt
    26581 tctactgctg gtggttattc ttttccaagt gctcatactc aaactgcaac aacattttgg
    26641 gttagtatta tgactatatt taaaaagaga tatcttcatt taataggtgc agttatggtt
    26701 ttaggagtag gattgtctag gctgtattta gctgttcatt ggccaataga tgtgatagcg
    26761 ggttggatat taggaatatt ttttacagtt gtatttgtaa aaatatttga ctatattgat
    26821 gataataaaa attatatttt acttgtagtt ttacttatac catttataat agtggctata
    26881 tttctaaata gccctgaata ttttgaaaaa tttggtataa tagtaggatt tgtatttgga
    26941 tatatggttg aggatagatt tgttaaattt aatacagata ataataacaa aaaaataaat
    27001 tttagcaata aaaataaggg gagtaaaaat ataatttttt caagaatatg taggtttttg
    27061 gttgggatag taacaatagg aatgttatat attggcatta agttgtttat ttcaatgtta
    27121 atagtcactt taaatataag tgactctact atgagtataa tttttatgaa ttttattaag
    27181 aatacagtag ttgtttttta tggaatagca ggagctccag cattatttaa acttttaaaa
    27241 ttaaattgat tttaaggtat agagtcaata gtttaaatta aaaaaattaa gggtagaaaa
    27301 gagtgattat ttgaagaagg tagtaataat aggtggtgga ccagcaggga tgatagccgc
    27361 atcaactgca tgtgagaaag gctatgatgt aacattaatt gagaaaaatc ataaattagg
    27421 aaaaaaactt gcaataacag gaaaaggtag atgtaatatt actaacgctt gtgaaataga
    27481 ggaattaata gaaaatgtac ctacaaatgg aaaatttctt tatagtgctt tttatacttt
    27541 tacaaatgat gatgtaataa gtatgtttaa taatcttgga gtaaagacta aaacagaaag
    27601 aggaaaaagg gtttttccag agagtgataa agcatttgat attgtaaatg ctcttgaaag
    27661 acaacttaaa agtaagaaag taaatatatt attaaattcg aaagtagaaa aaataatatc
    27721 taaaaataat aaaattgaaa aagtaatttt aaacgataaa aaagaaatta agtgtgattc
    27781 agttgtagta gcaactggtg gattaagtta tccacttact ggttctactg gtgatggata
    27841 taaatttgca atttcacagg ggcacacaat tatagataca aaaccatctt taattggtat
    27901 agaagtacaa gaaagtttta caaaagattt agaaaaatta tcattgagaa atgttgaaat
    27961 aagggtgttt aatagcaaac aaaagaaagt ttatagtgat tttggagaac ttgaatttac
    28021 taaatttggt ttagatggac ctataatcaa gtctgcaagt tgtagaatga aagatacaag
    28081 gaaagaaaat tacactatat tattagattt aaaacctgca ttagatgagg aaaaattgga
    28141 taaaagagtt caaaaagatt ttcagaagta tacaaataaa aaatttgaaa aagcattaga
    28201 tgatttactt ccaaaaaaac taatacctat aataataaac ttaagtgaaa ttaatccaaa
    28261 tacggttgtt caccaaattt ctagagagca gagaaaaaac ttagtccatt tattaaaaaa
    28321 tttaaagttt acagtaaaga gatacagacc tattgaagaa gctataataa cttctggtgg
    28381 tgtaaaagta aacgaaataa attcaagtac aatggaatca aaattagtag aaggtttatt
    28441 ttttgctgga gaagtaatag atatagatgc atatacaggt ggattcaatt tgcaaatagc
    28501 tttttctact ggatatttgg caggttttaa ttgctaaaaa ttacctgaat taaaaatgtt
    28561 taaaatcaca taatattatc agcaacacaa aataaattaa acagaaggaa ggtaatcaac
    28621 atggcaagta acaataacaa caacagaaca gtagtaccag gagcaaaaga ggctttaaac
    28681 caaatgaaat tagaaatagc taatgaactt ggtatgagta attatcaaca agttgataaa
    28741 ggaaacctta ctgcaagaga aaatggttat gtaggtggat acatgactaa aaaattagtt
    28801 gaaatggctg aacaacaaat ggctggaaaa agcaacagat agttataaat aattatttat
    28861 aactattggc tattagttta gctagagcta tctcaaatat agaaagtaat ttctaaattg
    28921 agatagcttt tttttattaa attattaaat agatatattt ttatgtaata aattttatgt
    28981 atttggataa aataatacaa atgaatatgt tacataagga gtgatgtaaa atgagtagtg
    29041 taaattttga ggatttaaaa aacaaattta ttaatagtga cttagatgaa aaaataagaa
    29101 tatatacaac aacagaaggt ttatcagtag aacaatttaa agagctacta aaatattatc
    29161 caatacaaca tttatctaaa ctagaaaaag cacttggata gcagtattta atccaaaatt
    29221 aaaaaggcaa tttatttaat attaaaaata ttttttatta aataaattgc ctttttataa
    29281 taggtttaaa ttattatact tttgatatat aattgtagat tctctcattt ccaagttcaa
    29341 tagacccagt attaataaga gtaactattc cttctatatc tctagcagtt aagtatttat
    29401 atggaattaa taaattataa ccagtattac ttagtaagtt ttttaattgg gttatttgag
    29461 agatattgaa acattttgca gttttagtag atttttttct taagttagat aaattttcat
    29521 tttgaatatt tattaaagtt tctatatagt ttctataaga tatcttaaag tttgtctcat
    29581 tgactatttt ttcattaagt atactactga taaaatcttg agacaatata ttatctgcaa
    29641 atttatctgc tgttaatttt ccagatgtta tgcctttttg atattttcta agaatttttt
    29701 tagtatagca gtatatttta tttctcgttt ctaaatcgag ttgttctatt tgcacaaaac
    29761 cacttccttc taaattataa aaatatttat taaaaatcta tacttaacta atcaagcttc
    29821 aataacttat ttacaataaa tactaaataa taaaatattt gttatttttt attattacca
    29881 agtataaaaa atattataat tgtaaaactt acaaatatat gtaaaatatt taggagtgat
    29941 gaaaatgtta aatgggataa taaagctttt ttattctgaa attgtaaaaa caaagattaa
    30001 taacttagat tcaagtggat attctttaag agatgaatgt aaagaaatca gaaatataac
    30061 tctaaagata gatagtgtat tttttaagct tttaaagtta gcttttggtt caatattgct
    30121 tatggtagct tttaatttaa acatggtttt aggtatagga gtgtttttag ttggtgtagt
    30181 ttatatacta tataaaagaa atatggaaga acaagtgaaa gagcatataa ataatgtgaa
    30241 aaataatatc gacatctcta aaataacttt tatggatgaa aaagggagaa caggtctaaa
    30301 tgcattaatt acattacttc ttataggatt attgacagga tttaactgga tattagttgc
    30361 tagttttgtc gttgtttttg tttttactat aaagaatgta tgttaaaata ctaataaata
    30421 gtttaatata tagggggaga aattgagtat gactaatggc agttataaaa tgaagaattt
    30481 agtggaaata tccgctatgg tgactgaatc tacagatttt tttaatataa aagatgacat
    30541 aatagaaaaa atgttagaag ttgttcaccc tacaaaggca tgtgtgaatc tattttataa
    30601 aaatgattct aaatatgcct acttagtatg ttcgcaaacc ttagaatatg tacctcaaat
    30661 ttttccaata aatagtctta aaggtgcaaa aattgatttt gacacatatc cagagtatat
    30721 acatgaggct gtaaatgaaa aaaagataat atatgtggaa aatgtatttg aagattctag
    30781 agcagaaggt gaacgagatt tagcaaaagc tgaaggttat attgggagaa tagtttttcc
    30841 ttttataata aataacgtag ttgttggatt tatgacttgt tttttaaaag aggatgatta
    30901 tataacagaa caagatatag attttatatc atctgttgca tcactcttaa gtttatcaat
    30961 agaaataact aataaaaata ataatgtaca aattttaatt gataaactaa gaggctcaat
    31021 aagtgccata aatgaagcta ctaaaaaact ataccttaat aagagtattg atggcttttt
    31081 ggaaaattta agtaaacaag cgtgtaatat tactaatagt aaagaagctt taataattat
    31141 aaatagtcat cataataagg atgctatagt tagttgttat aatgttgagc aaaaaaagaa
    31201 aacaaattta tatcctatga ttgatatgtt tttaaagaaa gagtcattgg gggggtatct
    31261 aaataattta aaaccatgct caaaaaaggg aataaatttg gaaagttata tatattacaa
    31321 acttcaggat aaaaatgata ctataggatg tatagtttgt gcaaatagca aaaactatac
    31381 tagtgatgat ttaaatattc ttagtatatt gtccaaacaa gtttctgttg gtatgcaatt
    31441 atatgaacat aatgaaaagg aagtaaaaca caaagttctt gaaaacgagt tgaacatatt
    31501 aaataaacaa caacagctta ttatggataa aggtaaaatg gattgtaatg atgagaaaga
    31561 actttatttt taccataaac cagcaagggt agtaggtgga gatttttatt atgctacaaa
    31621 agtggatgac gaacatatag tttatattgt tgctgatgta atgggacatg gaatggtatc
    31681 aaattatgtt gttgcaatga taaaaggtgc ttttaaagta ttatgcaatc aatacaatac
    31741 accaagagaa ataatgacaa atttaaatag gatgttatat gatgaatttg acaagatggg
    31801 agtatttaca acttgtttga taggtattat agatactaaa gaaaatatga ttactgtgtc
    31861 taatgcaggt cactatagtc ctgtagttgt aaagaaagat ggcacaatag aaaataattt
    31921 aaattgtaaa aagggcatac ctattggaat aatggaagat gctacttatg aaaataatac
    31981 tctttcaatg gaagattatg caatggtatg tatgtacact gatggaattt tagagataaa
    32041 aaattcatca aaagaagagt atggtataaa ccgattagaa aactttctaa aagaaaattt
    32101 tagattaaat caacagttaa tagtagaaaa tctaaaattg gatttaaaga acttctcatc
    32161 aaaagataat tatgatgatg atattttaat agttatgtta aaggataggt agattgatta
    32221 tggattcaaa cgataaaaaa ggacttaaaa gtccagagat tataataata aatggaagtc
    32281 caagaattaa taaaaattgc agttttatag caaatgaagt aaaagctatg ctagaagagt
    32341 gtggattaag ttgtaaaata tttaatatct acaatatgga tattgaatat tgcacagcat
    32401 gtggattttg tgaaaaaaca gggtattgca agtttaatga tgatatgaag ccattatata
    32461 aaatgtttga tgattcaaaa ggaactatag taataagtcc tgtacatttt gattcaatat
    32521 cagctaaatt aaagactgtt gttgatagaa ctcaagctat atatgcaagt aaatatgtat
    32581 taaataaacc ttcaattgat agaagtaaga aacggatagg aatgtatatt gcagtggggg
    32641 ggagtaaatc atatgattca cagtttaaag gtggacagat tgtgatggat tttttcttta
    32701 agagtataaa tactaaactt ttacacaata tatatttaaa caatacagat gatgtatctt
    32761 atatagataa tcaagaattt aaagatataa tatataaaaa tttaaagata tatattgatt
    32821 caatataatt tgattttgta ggtaatatta tgtataattg tattgcaata atttaaataa
    32881 ttaaaaggag ggaatttcaa tgaaattacc aatagcatta tctaataaac atgttcattt
    32941 gagtcaagct gatatagacg tattatttgg ggcaggtcat gagttaacta aatttaaacc
    33001 attatctcaa ccagggcaat atgcctgtga agaaaaagtg gatttagtag gaccaaaagg
    33061 aactttaaaa ggagttagag tactaggacc agctagagct catactcaag ttgaaatatc
    33121 tttagcagat ggtttcccat taggagtaaa agcaccaata aaagaatctg gtgatatagc
    33181 tggaactcca ggtgttaaat tagtaggacc tgctggagaa gttgaaatga aagaaggagt
    33241 tatagttgct tctagacata tacatatgag caatgaagat gctgctaaat ttggtgtaac
    33301 tgataagcaa atagtaaaag ttaaaacatc tggaccaaga gcattagtat ttgaaaatgt
    33361 attagtaaga gctagtgaca gctttaatct agaaatgcat gttgatatgg aagagggaaa
    33421 tgcagcaggc gttaaaaatg gagatttagt agaattaata gttgattaat attataaata
    33481 gactttatgc tagtagatat tttatttgct agcatatttt taaatgtaat aaatttctat
    33541 aggataaatt taatcgatta gattaattta taaaataaag gggtattata aaaaatgtaa
    33601 taaaaactta caaaaatatg atgtaatatg gtaaaattat atatataaaa atttacatgt
    33661 aataaggata agcattttac tttttatact aatatgaaca aatagataaa aaagtttgaa
    33721 aaatctaact taatttatta tatattgtat acaaaattat gaaaaaaact taacatattt
    33781 aattttaaaa aa
//
LOCUS       DAELTA010000025        33629 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.025, whole genome shotgun sequence.
ACCESSION   DAELTA010000025 DAELTA010000000
VERSION     DAELTA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.025"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1181)
                     /locus_tag="KPG13_001025"
     CDS             complement(<1..1181)
                     /locus_tag="KPG13_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0984766.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     Y"
     gene            complement(1274..2146)
                     /locus_tag="KPG13_001026"
     CDS             complement(1274..2146)
                     /locus_tag="KPG13_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG0984767.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(2248..2583)
                     /gene="fliT"
                     /locus_tag="KPG13_001027"
     CDS             complement(2248..2583)
                     /gene="fliT"
                     /locus_tag="KPG13_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG0984768.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            complement(2586..4109)
                     /gene="fliD"
                     /locus_tag="KPG13_001028"
     CDS             complement(2586..4109)
                     /gene="fliD"
                     /locus_tag="KPG13_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG0984769.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(4130..4486)
                     /gene="fliS"
                     /locus_tag="KPG13_001029"
     CDS             complement(4130..4486)
                     /gene="fliS"
                     /locus_tag="KPG13_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0984770.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            complement(4486..4881)
                     /gene="fliS"
                     /locus_tag="KPG13_001030"
     CDS             complement(4486..4881)
                     /gene="fliS"
                     /locus_tag="KPG13_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG0984771.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(4903..5115)
                     /gene="csrA"
                     /locus_tag="KPG13_001031"
     CDS             complement(4903..5115)
                     /gene="csrA"
                     /locus_tag="KPG13_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG0984772.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            complement(5109..5501)
                     /locus_tag="KPG13_001032"
     CDS             complement(5109..5501)
                     /locus_tag="KPG13_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG0984773.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            complement(5517..6449)
                     /gene="flgL"
                     /locus_tag="KPG13_001033"
     CDS             complement(5517..6449)
                     /gene="flgL"
                     /locus_tag="KPG13_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG0984774.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            complement(6468..7778)
                     /gene="flgK"
                     /locus_tag="KPG13_001034"
     CDS             complement(6468..7778)
                     /gene="flgK"
                     /locus_tag="KPG13_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG0984775.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(7796..8212)
                     /locus_tag="KPG13_001035"
     CDS             complement(7796..8212)
                     /locus_tag="KPG13_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG0984776.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            complement(8217..8492)
                     /gene="flgM"
                     /locus_tag="KPG13_001036"
     CDS             complement(8217..8492)
                     /gene="flgM"
                     /locus_tag="KPG13_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG0984777.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(8643..8957)
                     /locus_tag="KPG13_001037"
     CDS             complement(8643..8957)
                     /locus_tag="KPG13_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0984778.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            complement(8981..9409)
                     /locus_tag="KPG13_001038"
     CDS             complement(8981..9409)
                     /locus_tag="KPG13_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG0984779.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(9418..10086)
                     /locus_tag="KPG13_001039"
     CDS             complement(9418..10086)
                     /locus_tag="KPG13_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG0984780.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(10116..11099)
                     /gene="rfbB"
                     /locus_tag="KPG13_001040"
     CDS             complement(10116..11099)
                     /gene="rfbB"
                     /locus_tag="KPG13_001040"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG0984781.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(11137..11694)
                     /gene="rfbC"
                     /locus_tag="KPG13_001041"
     CDS             complement(11137..11694)
                     /gene="rfbC"
                     /locus_tag="KPG13_001041"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG0984782.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            complement(11743..12612)
                     /gene="rfbA"
                     /locus_tag="KPG13_001042"
     CDS             complement(11743..12612)
                     /gene="rfbA"
                     /locus_tag="KPG13_001042"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG0984783.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(12721..13593)
                     /gene="rfbD"
                     /locus_tag="KPG13_001043"
     CDS             complement(12721..13593)
                     /gene="rfbD"
                     /locus_tag="KPG13_001043"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG0984784.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(13799..18880)
                     /locus_tag="KPG13_001044"
                     /pseudo
     CDS             complement(13799..18880)
                     /locus_tag="KPG13_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(18891..20423)
                     /gene="purH"
                     /locus_tag="KPG13_001045"
     CDS             complement(18891..20423)
                     /gene="purH"
                     /locus_tag="KPG13_001045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG0984785.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(20416..21009)
                     /locus_tag="KPG13_001046"
     CDS             complement(20416..21009)
                     /locus_tag="KPG13_001046"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG0984786.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(21003..22079)
                     /locus_tag="KPG13_001047"
     CDS             complement(21003..22079)
                     /locus_tag="KPG13_001047"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG0984787.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(22073..23440)
                     /gene="purF"
                     /locus_tag="KPG13_001048"
     CDS             complement(22073..23440)
                     /gene="purF"
                     /locus_tag="KPG13_001048"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG0984788.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(23452..24150)
                     /locus_tag="KPG13_001049"
     CDS             complement(23452..24150)
                     /locus_tag="KPG13_001049"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0984789.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(24156..24632)
                     /gene="purE"
                     /locus_tag="KPG13_001050"
     CDS             complement(24156..24632)
                     /gene="purE"
                     /locus_tag="KPG13_001050"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG0984790.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            25082..25594
                     /locus_tag="KPG13_001051"
     CDS             25082..25594
                     /locus_tag="KPG13_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0984791.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            complement(25978..27141)
                     /locus_tag="KPG13_001052"
     CDS             complement(25978..27141)
                     /locus_tag="KPG13_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0984792.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(27138..27536)
                     /gene="tnpA"
                     /locus_tag="KPG13_001053"
     CDS             complement(27138..27536)
                     /gene="tnpA"
                     /locus_tag="KPG13_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0984793.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            27792..29228
                     /locus_tag="KPG13_001054"
     CDS             27792..29228
                     /locus_tag="KPG13_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG0984794.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(29320..31194)
                     /gene="asnB"
                     /locus_tag="KPG13_001055"
     CDS             complement(29320..31194)
                     /gene="asnB"
                     /locus_tag="KPG13_001055"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0984795.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            31397..32071
                     /locus_tag="KPG13_001056"
     CDS             31397..32071
                     /locus_tag="KPG13_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG0984796.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            32184..32720
                     /locus_tag="KPG13_001057"
     CDS             32184..32720
                     /locus_tag="KPG13_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG0984797.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(32886..33506)
                     /locus_tag="KPG13_001058"
     CDS             complement(32886..33506)
                     /locus_tag="KPG13_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG0984798.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT        10339 a         5991 c         3641 g        13658 t
ORIGIN      
        1 ctataaaaac tatttttttc aattttagaa tttgaaactt ctttataata attttttata
       61 tcttcaaatc tattcaattt atatagtgac ataaataagc taaagtacac attctttttc
      121 ttttccacat cttcaatgtt attatactct gaaattacaa actcatattt ttcaagctta
      181 taataaatct ttatcagtgt aataatagcc tcatctctaa gccctacagt atcagtgtct
      241 gaaaataaac tatttgcttg tgtacttatt tcataattat ctaacaagta aatatatctc
      301 ttatacgaat ctatactatt ttcatacttt cctaaatcta cttgtgcttg tgcaatataa
      361 taatatatat caatattatt catgtctttc ttaatatact ttaagcacaa tctttcacac
      421 ttcttatgtt ttcctaaata taaatatgtt tttacaaggc ttgttatcaa gtaaccaggt
      481 gccaattcta gtttagaata taattcataa gatttctcaa gatagtataa agcctcctga
      541 tattttccta atataataaa atttttccct aattgatagt ttgtatatgg agaattatca
      601 ttctctttta actcttgaaa taatagtttt tcatttcttt tcaccttatt taatcttatt
      661 tcttcatctt caaacaaata tccataatgt tcaaaatcag caacattatt aaatataggg
      721 tttttgtata ttggttgttc atgtacttta ccatggtatc taaaatcaac gtttttaaaa
      781 agtctaagca ctgaacaata tccatataat tttttatcat atgagtaaag attttttaat
      841 tgtattgaag cagaattaaa ttttttatca agaccatctt taaaaaattt tacaatagtt
      901 gaagaatcaa ttagtatttc atctgcatct aatataagta accattcacc tttagcatag
      961 gatattgata tatttctcat actagaaaaa tcattattcc aattatgaaa atacactttt
     1021 tccgtgtact tcttagatat ttttatagta tcatctgtag aacctgtatc gactattata
     1081 agttcacttt ctatagaatt tcttaatgtg gttaatgatt ttaaagtttt ttcaagtatt
     1141 ttttcttcat ttttaacaat cataactata ctaagtaaca tataaatttt cctttcaaaa
     1201 gtatatttgt aaaaaaagaa aggagtaaag caaagcctta ccctttcttc tatatccaat
     1261 atacaaaatt attttatcct aataattgta aaactccttg tggttgttga ttagcttgag
     1321 caagcattga ctgtgatgct tgaactaata tattcatttt agataagtta accatttctg
     1381 aagcaacatc tgtatctctt attcttgatt cagctgctgt aacattttct atagtattat
     1441 ttaagttgtt ttgagttgat tctagtctat tttgttgtgc ccctaatttt gctcttgatg
     1501 tatttaattc tttaagagct acatctaagc tagataccat ttgtttagct gcatttgttc
     1561 cagaagttcc atctgcattt aagcttgcta ttgtagaact tgttatacct gctttactca
     1621 ttatacttga agtatttact agagcaactt ttatttcatt gttgttgtta gatgttcctg
     1681 caacatttgt tccaaagttt gctccaactt gtaatcttat ttctgtagaa cttccatcaa
     1741 gtaatttctt accattaaac tcaattgagc ttgatattct ttcaacttca tcttttaatt
     1801 gtagtaactc atcagctatc ttttgtcttt cttcagcagt attagtttca tttgaagatt
     1861 gtactgataa agttctcatt ctttgtagta tgttaccagt ttcttctaat gcaccttctg
     1921 ctgtttgtac aacagatata ccatcttgaa cgtttcttcc tgcttggtct aaacctttta
     1981 tttgagctct cattttctca gatatagcaa gcccagcagc atcgtcagca gctctcttaa
     2041 ttctaacacc agaagatagc ttctccatag acttactttg tgcatttacg tttcttccca
     2101 tttggttatt tgctatcaaa gcacttacat ttgtattaac tctcattatt ttaccctcct
     2161 tagtatagtt gacatccttg tcatttctac ttttaatctt ttttattaat tcttcattta
     2221 tataatcgga caagatggta cataacttta tactttttta ttaaaaatat ttactttttc
     2281 tctatttaag ttataataca ttgtgttcac ttgtttagta agcttatact catgtaattc
     2341 tttttttact tctaataatt gtccttgtaa aatatctctt atcctgctat caatttcaat
     2401 taattccatt ctattataaa gctgtataaa atcatttcta tcaatttcac ttacactatt
     2461 tataatctct tgtctttcac ctaacttaat acttatcttt atatattctt ccttttcaat
     2521 caaatttata atttgtaaag atatatcttt atataaattt aatttttctt ttaatttatc
     2581 cataattaat taccctgtgc ttgtgagaaa taattcattt gggaagaata ctgattcaac
     2641 aaagattcta aggctgaata tttagcataa agtcttttct ctttatcatt cattttttca
     2701 actaacctgc ttatatttct ttcttgctct gcaatttgtt ttgaatatac attttcacta
     2761 gcagaagcag ttttttctat acctgctttt ttaggaagtt tagaagttga acttccagaa
     2821 tattcatata caatatcttt tagcttttca aacattccct tatcatcata attcgaagaa
     2881 taacctgcta gtgttttgta taccttatca ctatcatcta taagagcctt tgaaaaagta
     2941 tctgcattaa atgatatttg acctttttta ttgtaatttt cactttcatt gatacctagt
     3001 tttcttaatg caataatatt ttttccatct ccaaaaaatg cagattgaat atcttcaaca
     3061 aaacctctta actcatcatc atttctaagt ataccttctt tagctttctt ttcccatttt
     3121 tctatttctt cagttgtcat atcttctttt tgggcatctg taagaggcgg ataatctttt
     3181 ggttttttag tagtaactaa accatagacc ttgtccatca gtttattata atcatccaca
     3241 aatgcttgca tatttttaac agcttcttca gtatcagtga cagaagtcaa ctttgcagtt
     3301 ccttttgaat ttacattata gtcaatatta tctatattaa atgaattttg ttctttactt
     3361 atatgagtta ctcttccatg ttcatcagtt acatcagctt ctagattctt tccatataca
     3421 tttgccactt tgtttccacc agcatcattt ataaatgtga taccattatc attagcaatt
     3481 tgctgagcta aattgtcatt tccaattact cttaaattaa tgtctgatga cgaaccagtc
     3541 tgttttcttg aaataatcaa ttcacctgtc atctcactat aagaagcctt tatatcatta
     3601 tctgcgcaaa gggagtttat cttacttaca atagtacttg tagtatcttg ttcagatatt
     3661 ggtatattta catctcctat agttaaagaa ctcgctccac tggcatttgg agggaactgt
     3721 cgaacaatag gttcacttga atttaattta atagtaaatt ttgctggttc agccatttga
     3781 gaaactgcaa attgataatt tattttttct gcaccagctg aaccttttgc tactataata
     3841 ctttcatcag aactggttat tcttgtagaa gagtatgctt tttcacttac tatactatta
     3901 ggagaattta tgcttagata tttatcataa agatcttttg attcttgtat aacatttcta
     3961 tatatttctt gcttccattt aacgatttgt tgttcttgtt ttgctttatc aactttttcc
     4021 ttgtctctaa tcatactagc ttcgattatg ccttccatat caaaatttcc tgaaagacct
     4081 gtaactctta taggacttat acttgacata cctttatccc tccatctaat tatattttta
     4141 gttgattgta aacttcatgc catgtatctc taatttgttt tactattggc aatatatcat
     4201 caattatttt aatatcttta ttttcatcag ctattgctaa ttcagatttt ataaattcat
     4261 aaactttata catatccttt gcccattgtc ctgcattttg gtctaatgta gtcattagtt
     4321 ctgtaaatat attttgaact cttacaagtt cattatgtgc tttcaaatag tcgttattta
     4381 aaagagcttc tttggatata ttggtatatt ttactgtgcc atctacaagc atcaatagta
     4441 attcctcttt tgataactta tctaattcag ctaaatttaa ttccattatt tgtacctact
     4501 tctttcttct ttactttttt tatcagcttc atgccatgta tctttaattt gttctattac
     4561 aggtaacaca ttatctatta cctggatgtc ttttctaata ttggctcttg aaagttcata
     4621 tcttacaaaa tcataaactt tgtacatatc ctttgcccat tgtcctgcat tttggtctaa
     4681 tgtaaccatt agttctgtaa atatatcctg aactcttaca agttctctat gcgctttctg
     4741 tgtattttta tctaagatag ctgctctggc tattttggta tattttactg ctccatcaac
     4801 aagcataagt aacaattgtt cctttgatgc cataaaaaca gcattttgtt tataagaatt
     4861 ataaggattt tctccataca tatattactt cctccaagct tattatttta atgactttaa
     4921 aatttttata tttttatttg tagcttttat attctcactc tttacttttt cgtatatttc
     4981 ttttcttaat atacttatat tgttaggcgc tgatatagct agttttacat tgtttccatc
     5041 tactcctaca atttttactt ctatgctttc tcctataagt actgcttcat ctttttttct
     5101 tgaaattact agcatccatt atcacctctc aataatggat gccttattga atatttatca
     5161 tcctgtaaaa ttatttgcat tccaaaatta gtttttatat ttatcactat aggagctttc
     5221 atgttcacag ttgattcttc aaatgtctga ccaagagtaa ttatattaaa taatacaaca
     5281 tcctttggag atttcacttc tagtttttct attgtttcct gatttaaaac tatttcatat
     5341 tcattgataa tctcaaatgg agatacaaca acaaatccta taccttcttc ttcagtagaa
     5401 ttaaattctt ttaaataatc attatcttct atatctaaaa gctcatattc ttttaaattt
     5461 tcaaaaccta atataccttt ttttaatgta accttcatca tatctgcgtt attttattat
     5521 atgtaatcta atatagatac attaaataac tttcctcctg tttgtagtga agcttgataa
     5581 gttaactctg ctgcctttaa ttgaacaagt ttttcaccat aatctatttc ttcattttgt
     5641 gacaaaatat cctttaactg tatcaaacta tcatcgtttt tttccttcat agcctctact
     5701 gtatttgctt ttgtcccata tatagaccta acatttactg tattttctat tagttgttcc
     5761 atatcgccca aacaagttgt tgtaagctct tttatagcat cagcatctgg atttgctaat
     5821 ttttttgata tgttatttaa ctcttcaaat gggtcagttt ttgttgggtc aaatccaagg
     5881 ctactcaatt taacgttata atctacactt attccttgag ctatatccat actcaaagag
     5941 tcatttaatt tattaagatt tagactagaa ttaaacttta aactcactga accatcagca
     6001 ttatctatac attcaacagg tggtaccccc gtatttgaac ctgaaaatat gtacttccct
     6061 tcatgtgttg catttaaagc atttgcaatt tctttcattt tttcattagt ttcattacta
     6121 agagtcttta attcatcatc tgagtatgca ccatcaccac ttgccaccaa agttttcttt
     6181 atgtctgata ataattttcc aagctgatct aatgcagtat cagtagtttc taaccaattc
     6241 cctgtatctt tacaattctc aacataagtc tccatagtct ttatttcact ttttgagttt
     6301 aatatcttta ttgctttata tggattatct gatagcttgt ttatttcttt tgttgtatta
     6361 acttgtctat taaccttatc caatctttgt agattatctt gtagtgagtt tagatagctg
     6421 ctagacatca tacctgtact gactctcatt ataaattccc ccttctatta aattattcca
     6481 tttattacaa catccagtag ttgcacagct gttgacataa cttttgcact agcttgatat
     6541 gaacgttgta tttttataag ctctgtcatt tcttcatcca aagatacacc tgaaacactc
     6601 attcttgagt tatcaatatt atttataatt ttacgttgat ttgcttcatc ttgatttact
     6661 ttttgagaat ctaaaccgat tgattctgca aaggctttat agtggtcact tattgtactt
     6721 acttttccac cagccacccc aatatctatt ttttcatcct ttaacttagc aatttttgaa
     6781 gcttcatcag cagtaagctt taattcattt gggttactct ttatagctgg atttatcttt
     6841 ataatatctt caccatctac atttgaagtt ataaaaaaat ctgttactgt tattcctgca
     6901 gacgtattat aaacatcatt tatctgttta gctaatccat cagatacatc ttttaaatcg
     6961 cttttatatg tatcaatctt accatgcatt tcaaacaagc cttgtaaggt ccctgatata
     7021 cctgttttcc aagttccatc tggatttttt aattcattaa gttctaattt accatctgca
     7081 agcatatttt taacatcact attttcaaag tctagtttaa aactcaagtt atctaaaatt
     7141 ctatctcttt catcaagcaa atcatttggt gtagagccag aaccagatat aatatcaata
     7201 gatttattta aatcttcaag actttttaac atgtcattta tttgttttat ctcagtgtct
     7261 atattagaat ctatatcttc ttttaactta tcaagcttct tataactttc acttattaaa
     7321 gttgctaaat tagatgcgtt ttctaccact agactttttt tactgtcact atttatatcc
     7381 tttgataatg actcccatcc tttaaaaaaa tcatctatac ctgcagatat tccatagtct
     7441 gatggttcgt ttagtatagt ttctatagta ttgtaataat catattttga aactgtctta
     7501 ccatacttat gagcttctgc cctaaattgg taatcataaa aactatttct agctcttgtt
     7561 acatccgtta catgtactcc agtccctaat tgtcctcttg caattgaatt actcaaacta
     7621 ggcatataca ttggactatt tgtagcaaac tcggtccttt gcctagaata accgtctgta
     7681 tctagattac ttatattatg agaaattgtc tgtattgctt tttggtgcgc attcattcct
     7741 gaattagctg tatttaaaat ggaaaataat cctgccatat ttctcctcct aatatttatt
     7801 ttctaatatt tccataagaa ttataagttg tagtttgctt atcagctggt tttatgaaat
     7861 taatcatttt tttcgtaaaa ataagttctt tctttattag agaatcattt gattcttgtt
     7921 gatttttagc taaagcaaga atgcgtttca agtctgtata tatttctttt aaatatccat
     7981 cattactatt ttcaacaact tcactcattg aaatatcatt accaaaaagt tgtcttctct
     8041 ttatttctat ggatgcaagc tccctgctaa tatcttctag tttaggattt aacatattta
     8101 aattttctaa gtctttttca actatataat caaattgttc ttttaataat tccatcatct
     8161 tactaattat atttttttct tcatatatta taacttttaa gtctgtaatc atgactttat
     8221 cctcgcatat cctctatcat ttttttcgca atttcttttg gttttatttt gtaagttcca
     8281 ttttctaatg cattttttat ttcatcgact tttttatttc taatattctc atcactcata
     8341 tttgacacac ttgccatttc ctttgcaaat ttagataact ctatcttgtc tgttttatta
     8401 atttttttaa cctcttccac tttttttata ccagatgtat tctttaaata attattcact
     8461 acaatatttg aacttacatt tttaatatcc atagtttcac ctccaaaatt acatacttat
     8521 aatttagtta tcgctcataa aataaaaata tttatataat tcacacatat tttaaatttt
     8581 ttataaaaat atccccttag ctaaactcat acagaataga ctaaagggat atatttaaaa
     8641 tgctaattta catctgaatc ttctatttta gataatctta cagataactt ctcatttgta
     8701 ataatgcttt ctccataagc aaacaatcta tcgtttgcat atatatctaa tttttcatcc
     8761 acattcttac ttaattttat tatagagcct tcttttaaat ctaatatatt ttttattgtt
     8821 tctgttgtat ttcctataga aacagctacc tttacttttg cattaaaaac attttgattt
     8881 tcttctataa caccagattt ttcttctatt aattgcccaa aattcaattc atatatatcc
     8941 tcttttttgc tattcataaa atttttctcc ttgtataacc ttatctatac atatctatca
     9001 ataagccttg tatcatgtca acagtagaaa caatatctat gttttctttt tcttcactaa
     9061 ttaacttact agtcatatcc tcaagtttac tatcaacaat ctttacattc ttataatact
     9121 gatgctccca aaagctacta tttttatcaa gtgaatacat attatctaca acagatttta
     9181 ggtattcttt tactgcattt ttatatatac taatatcgcc atagttttga gtaagtacaa
     9241 gcctttctcc tgcctttttt acactttcca aatttcgttt tatatcttcc aatgaagaag
     9301 attgttttaa agagtttaat ttgtcagaaa atttattact ctcttttagt tttttaacct
     9361 ccatatttat acttggactt atgtttagct tatctatatt tattcccata ataaagccta
     9421 ccctctataa tatttcataa ccttaggaat ataattttgt gtttcttttg gcattttata
     9481 caggtcagag attgattcta ctcctctact tttcattctt ccaggaccac cattatatgc
     9541 catcaatccc atttctatgc tccctccaaa catatcaatg tattccttaa tatgtctagt
     9601 tccagcatct atattctgtt ctatattaaa agggtctgta acccctaagt ctctgcagtt
     9661 ttctggcata agttgcataa gacccttagc accagcagaa gaaacggtat ttggattaaa
     9721 atctgattca actttaatta ttgcttttat aagatttgaa tctacgttat atttttttga
     9781 tgcttgattt acagcatttt caattctcaa ctttacattt ttatcttttg tatcaacatc
     9841 tataaatcta ctagtggtat tatttactac attatcaagt tcattaaata aattctcaga
     9901 attagacaat aaattgtagc tattagaagt atgttgatta ttttgtgata atgcttttaa
     9961 caaggtcatc ataatcatat caaatccaga tgaattatta tatgtacaat tacattgatt
    10021 tgtagtatta ctaccagata attgagtcaa tgctaatata ttaattagtg aacttatctc
    10081 attcaatata aaacgcccct ttctaaattt taaagttata ctgataaaac agctttccac
    10141 caagattcat tttctatata ccacttaaca gtttctttta tacccaaatc aaagtcatat
    10201 tttgccttcc aacctaactc attctctatt ttagttgcat caatagcata tcttaaatca
    10261 tgaccaggtc tatcatttac ataggatatt aactcttcag atttacctaa aagatttaat
    10321 atcattttta ctacttctag attacttctt tcattatgac ctcctatatt gtaaatctcc
    10381 cctatatttc ctttatgtat tattaagtct atagcagtac aatggtcata tacatgtaac
    10441 caatctctta cattttcccc attcccatat actggcaatt gtttgttttc caaagcattt
    10501 aaaatcataa gaggaatcaa tttttcagga aaatgatatg gcccataatt gttggaacac
    10561 ctagatatag ttgttaacaa tccatatgtt ctataataag aacttaccaa taaatctgct
    10621 gatgctttag aagctgaata tggagaactt ggattgatag aagattgttc tgtaaagaat
    10681 aaatcctttc tatcaattgg taaatcacca tacacttcgt ctgtagatat ttgatgatac
    10741 cttttaactc catattttat agatgcatct aaaagtactt gtgttcctat aatgtttgtt
    10801 ttaataaata aatcaggatt ctcaactgac ctatctacat gagattcagc agcaaagtta
    10861 acaactatat caaacctttc ttcttcaaat agcttataca cctgctccct atttgaaata
    10921 tctcctttaa caaatttata attggaattg tcttgtattt ctattaatgt ctctaaatta
    10981 ccagcataag tcaacaagtc taggttaaca ataaaataat ctacatactt gttaagcata
    11041 taatgtataa aattactccc aataaatcct gctcctccag ttatcagtat ttttttcata
    11101 ttttttctcc taaatgttaa ttctaataaa tttttattaa cgtcttgtac aaatatcttt
    11161 aaaacttttt tgttttttgt ccttttctga taaaaatatt tcttcaattc cttttaaagg
    11221 ccactcaata gatatttgct tgtcattcca tataaagcca ttctcaaatt ctgggtaata
    11281 ataatttgta catttgtaac taaatactgc tttttctgat aaaactaaaa atccatgtgc
    11341 aaacccttct ggaatataaa attgtttttt attcttatca gacaatatta caccttccca
    11401 tttcccaaat gtttgtgaat ctttccttaa atcaactgca acatcgaaaa cttctccctc
    11461 tgtaactctg actaattttc cttgaggata tttaacctga aaatgcattc ctcttaaaac
    11521 gccttttctt gagctagact cattatcttg aataaaacac atatcaagac cctgctctat
    11581 aaaatccttt ttattatagc tttccataaa atatcctctt tcatcataaa atacatttgg
    11641 ttcgactata tataatcctt ctattttagt ctttataaca ttgatttttc ccattataat
    11701 aaactccctt aataaaattt ataataaatt ttatctacca tattacatat cttcactagc
    11761 caattttaat agatattgac catattcagt ttttatcagt ggcttggcta gtttcattaa
    11821 ttgttctcta cttatatatc cttttgtata tgcaatctct tctaaacatg ctatatacag
    11881 tccctgtctt gtttgaacca cttcaacata attagaagca tccaatagac ctttatgtgt
    11941 accagtatca agccaagcca tacctcttcc tagaagttcc acttttagtt ttcctcgttt
    12001 tagatattca ctgtttatag aggttatttc taattcacct ctatctgacg gttcaactag
    12061 ctttgctatc tctacaacat cattatcata aaaatataat cctggtatag cataattaga
    12121 tttaggaact gtaggttttt cttctattga caatacatta aaatccttat caaattctac
    12181 aactccaaac gaatttggat tacttacatg atacccaaat atagttgctc cttttctttt
    12241 aactgcattc tccaaccttt cactaaatcc atatccataa aatatattat cccctaatac
    12301 aagagacact ctgtcatttc ctataaattc ttctcctatt ataaacgctt ctgcaattcc
    12361 atttgctttc tcctgaattg catatgttat gttaagccct agtgaacttc catctccaaa
    12421 taattcttca aatgctttta tatctcttgg tgtagatatt attaatatat cttgtatccc
    12481 tgccaacata agaaccgaca tagggtaata tatcattggt ttatcgtata ttggaagtgt
    12541 ttgttttgat atacattttg ttataggata tagtcttgta ccagaccctc ctgctaaaat
    12601 aattcctttc atctttttcc atccttaaat ttgtttttgt catattatga ttatcggata
    12661 aataaaagaa atatttatat tttttataat atcttatttt cttttctcaa aataatacat
    12721 ttacttttca tttatatcat atctttctaa atattcttca atggattctt cccactttct
    12781 aaaagaattt aaaccaatta acttcaacat aaaattatcc aatacagaat atagtggtct
    12841 tggagcctta catttaaatt cgttactctt aattggattt acttttatat ctatattttt
    12901 tagtttaaat atctttttgg caaaatcata ccaactacac tcaccttcac atgtagcatg
    12961 ataaactcca taatgttctg tttccatgat ttttattata atttttgcta agtctactgt
    13021 acttgttgga gttccaaact ggtcattaac cacattaacc tctttatttt ctaaagaaag
    13081 ttttatcatg gtctttacaa aattattccc attaccatat aaccatgctg ttctcaatat
    13141 aaagtactta tgtgagaatt gttcaacaaa tttttctccc ataagtttac tttttccata
    13201 tacagaattg ggttcagttt tattgtcttc cctatatgga tatttagaaa aaccattgaa
    13261 tacatagtca gttgatatgt gaataagctt agcattaact ttttctgatg ctattgctag
    13321 atttctcact cctaaagcat ttattttgta tgcaatttcg atattatctt cacatgcatc
    13381 aacctttgta tatgctgcac agtttacaac tacatcaggt ttattatgca aaataaaatt
    13441 atctacatta gtctgattgg atatgtccaa atcacttctt gttgtagcta atataacata
    13501 ttttggttgg tttatacttt gattgattgc ttctaattgg ttaaccaatt cttttcctaa
    13561 ttgaccatta gaccctgtta ttaatatttt cattaattat gttgtcctcc aataaatatt
    13621 caattttact taataattaa ttataacaca gcttgtattt taacttaggt tgaacttatt
    13681 atttcttaga taaataaaaa agagatgtaa tattaaatga ttatcatcat attattacat
    13741 ctctttaaat ctaaatatct aaattatata ttctagctat caattaaaca tcaaagcttt
    13801 atctaaaata tttaactcca gattcaaata tcttttggtc ttttccacct ataatattct
    13861 tatatacttc ctcaccaatt ctttcagaat gtcccatttt accaaacact ctaccatcag
    13921 cactagttat accttcaaca gcatagaatg aaccatttgg attaaactca atatcataag
    13981 tagcttcatt attaaagtca acatattgag ttgctacttg tccattagca attaattctc
    14041 tcataacatc ttcactggca acaaacttac cttctccatg agaaattgct atattatgaa
    14101 tatctccaac attagtttga gctaaccaag gagatttatt agatgaaatt ctagttctag
    14161 caatcttagc ttgatggcga ccaatattat tgtaagtaag tgttggagca ctttcacttg
    14221 gaactcttat ttctccatat ggaactaacc ctaatttaat taatacttgg aatccattac
    14281 agatacctaa cattagtcca tctttttgag ttaaaaattc atttatagct tcttgcactc
    14341 taggatttct aaatacagta gctataaatt taccagaacc atctggctca tcaccagcac
    14401 taaatccacc tggtaacatt attatttgag aagattttat ttcgtttact attgtatcta
    14461 tagaatcttc tatgtcttta taagttaaat tcttaaatac tcttatactt gcattagctc
    14521 ctgctctttc aaatgctctt gcagagtcat attcacagtt tgttccaggg aatgctggta
    14581 taaatactct tggagtagct atacttaatg atgatttttt agcttctcct tgtgatatat
    14641 aacttatagt ttctatcttt gattttactt cttctgtttt agttgggaat ataggctcta
    14701 aagtactaca atgtgcttta tataattcat ctaatgcaag ttcttcacct ttgataaata
    14761 tactagtttc tttaactgta cttccaagca caacataatt gtatccttct aacatactta
    14821 aatcatcatt tgctaattca agtactatat ttccataact agcctcaaat aatttatcat
    14881 ctgtaaatgc tttttcagcc tcttcactaa acttaaatcc tattttatta ccaaaagcca
    14941 ttttacttat tgcttcacct acacctgcaa atcctaatgc atatgttgat aaaactttat
    15001 ttccatgtat aagttctctt actttatcta agtttctctt taattcctca aaatctacaa
    15061 catcattttc tgccttgttt acacataaca ttactacttt tgagtctgct tttttaaatt
    15121 ctggagatac tactttcttc gcatctactg tgtcaacagc aaatgaaact aatgttggtg
    15181 gaacttcaat atctttaaat gtaccagaca tactgtcttt tccacctata gcaggtatct
    15241 caaatttact ttgtgcataa aatgctccta aaagtgctga gaatggttta ccccattttt
    15301 ctggattgtt accaagtttt tcaaaatact cttggaatgt aagtcttgtt gtactataat
    15361 ttcctccaat agcaaccaat ttacacacag attctactac tgcatacaag gcaccatgga
    15421 atgggctcca ttttcctacc tttggattgt atccataagt cattattgta gaagtattag
    15481 tttctcctcc aagtactggt atcttagcaa ccataccttg tgttggagtc gcttgatact
    15541 ttcctccaaa tggcataagt actgtattac caccaattgt attatcaaac atctctacta
    15601 atcctttttg agaacaaaca ttaaggtcag atagtacttt tgtaaacttg tctttcatac
    15661 ttgcacactt aacatctttt actatttcat tagagctaaa gaatgtattt tcttcatcta
    15721 ctttagtaac atgaacatca gtagtttgtt ttactccgtt tgtatctaaa aactctctat
    15781 ttatatctac aattgctttt ccattccaga acattcttaa atatcctgta tctgttactg
    15841 tagctacatg cgtagcttct aagttttctt ctactgctaa ttgtataaat ttatctttat
    15901 tttctttttt tatggctact gccatacgct cttgtgactc tgaaattgca agctcagttc
    15961 catcaagtcc atcatatttt ttaggaacta agtctaagtt tatatctaag ctatcagcaa
    16021 tctctcctat agcaacacaa actccacctg ctccaaaatc attacatctt tttatcattt
    16081 gagcaacttc tttatttcta aagaatcttt gtatttttct ttcgttagga gcatctcctt
    16141 tttgaacttc tgcactacaa gttaagattg actcttcact atgttcttta gatgaaccag
    16201 ttgctcctcc acagccatct cttcctgttt ttcctccaag aagaacgatt acatctcctg
    16261 cttcaggtct ttctcttact acattctctt ttggtgctgc tgctataact gcacctattt
    16321 ccattctctt tgcaacaaag ttttcatcat atacttctgc aacttgtcca gttgtaagac
    16381 ctatttggtt accatatgaa ctatatccat gagcagcttc tgtagttatt tttctttgca
    16441 taagttttcc aggtaaagta tcttctaaag tcgttcttgg gtcagcactt cctgtaacac
    16501 gcattgcttg ataaacataa cttcttcctg ataatgggtc tcttatagct cctcctaaac
    16561 aagtagctgc tccaccaaat ggttctattt cagttggatg gttgtgagtt tcatttttaa
    16621 acattactag atatttttcc atcttaccat caacttcaac atctacgttt atactacatg
    16681 catttatttc ttcactttca tctaggtcat ttaatttccc atttttcttt aattctttta
    16741 ctgcaacagt ggctatatcc attaaacata tatctttatg tctgttcaca tatacattgt
    16801 ctcttgagtt aagatacatt tgatacgctt cttttactat atcattaaac ttaccatctt
    16861 ctatctttac attttctatt tcagtcataa aagtagtatg tctacaatga tcagaccaat
    16921 aagtatctaa tacttttatt tctgttatag ttggatttct cttttctgta tctttaaagt
    16981 atttttgaac gtgttgtaag tctcctaaag tcattgctaa tcctttttca cttacaaatg
    17041 tttttaatcc attttcatct aaatctataa atccatctaa tacttcaact gttgttggta
    17101 tttcagtttc catttttaac gtttctggtt tttctaaaga tgcttctcta ctatctactg
    17161 gatttataca gtaatctttt attttagaaa actcttcatc agttattttg cctgataata
    17221 tataaacttt tgctgtatta atagcaggtc ttatcccttg atttacaatt tgtacacatt
    17281 gagctgccca atcacctctt tggtcgtatt gaccaggaag aaattctatt gcaaatactc
    17341 tttcatcatt taattttggt atttcttcat aatatactac atctaaattt ggttcagaga
    17401 atattgtctt tgctgcattt tcataaactt cttctgatat tccttctaca tcatatctat
    17461 ttaatattct taaattttct atattatcta tgtgtaaact ttctactaag tcttttttta
    17521 aagctttagc ctcaaggtca aaaccttctc ttttttcaac taatactctt ctaaccattg
    17581 agtctttatt ttcagtattc aacatacctt atcctccatt ttagaaaaat tatcttccta
    17641 tgtctgttct atagtgcatt ccttcaaagt ttatcttctt tatgttttca taaacttttt
    17701 ctgctgcatc cttaactgta gttgactttg cagttatacc aattactctt cctccattgg
    17761 taaccaaatt accatcaaac atcttagtgc cactatggaa tactactata tcatcatcca
    17821 agttttcaag accagttata attttccctt tttcataact gtctggatac cctcctgatg
    17881 taagcataac acatacagct tcatcatcac tatagtttat ttctatatct tttaacttat
    17941 tatctagtat agcttccatt atctcatgta aatctgtttc taatctaaat aaaacagatt
    18001 gtgtctcagg gtctccaaat cttacattat attcaagtac tttttctcca tcttcagtta
    18061 tcataagacc aacaaataaa atacctttaa aattaagtcc atcttttttg aagccttcta
    18121 aagttttttc taatactgtc tcttttactt tatttgctaa ttcttctgta tatatttcac
    18181 taggtgaaaa agttcccatt cccccagtgt ttggaccttg ctcatagttg tttacttttt
    18241 tatggtcttt tgcacttgcc ataggaacta ttgtatcatt atctacaaat gctagaattg
    18301 aagtttctat tccttttaaa aactcttcaa ttactatttt atctccagca gctccaaact
    18361 tcttatctga catcatttct tttaaagttg ctatagcatc ttctctattt tcaggtatta
    18421 caacaccttt tccagcagct aatccgtccg cttttattac tactggatat ccaaaactat
    18481 ctatttctga tatagcttct tctaagtttg tatattcttt atattttgca gtaggtatgt
    18541 tatgttttat cataaattct tttgaaaaag ctttacttcc ttcaagttga gcacattttt
    18601 tattaggtcc aaatactctt aatccttctt tttcaaactc atctactatt cccataacta
    18661 gtggtacttc tggtcctact attgtcagac ctatttcatt ttctttggca aattttaaaa
    18721 gttcttctat attagtatca cctatattta cacattctgc tacgtttgca attcctgcat
    18781 tacctggtgc acaataaatt ttttctacat ttttttcttt gcttagtttc cagcatatag
    18841 catgctctct tccgccaccg cctacaacca atatcttcat aatatcctcc ttagtgtttg
    18901 aagtgtctta ttcctgtaaa taccataacc atatcatact tgtcacaagc ttctattgaa
    18961 tctttatctt ttatagagcc acctggttga actactgctg atattccata ttcatgagct
    19021 aatgttacac aatcatcaaa tgggaaaaat gcgtctgacg ctaaaacagc tcctgtaaaa
    19081 tctttatctt tgttattttc aagagcattt tttaatgccc aaattcttga tgtttgtcca
    19141 caacctaaag ctagagtttg tccatttttt actattgcaa ttgcatttga tttcatattt
    19201 tttactactc tcataccaaa ttccatgtcc ttaagttgtg catctgttgg tttttccttt
    19261 gttacatttt cacttttttc tgctaatata ttgtttctat cttgtatcaa taattttcca
    19321 tctaaatatt tcatttcata tggttgtaaa ctattttcta ttttagctaa ttttaatatt
    19381 cttagatttt tcttttgttt aagtatctct aaagcttctg gttcaaaatc atatgctact
    19441 actatttcta agaatatttc atttaatttc tctgctgtag ctttatctat agtagaagtt
    19501 attcctacta ttcctccaaa tattgaaacc ttatccgctt cataacattt tgtatatgct
    19561 tcaaaagcat cttttcctaa acctacccca catgaattag catgttttat agctactgaa
    19621 actacttctt cactatcttt aaattctctc attacttcta aacatccatg taaatcattt
    19681 aagttgttaa aagaaagttc tttaccgcct aattgctcat aatttaagat tggatttttt
    19741 gcatttgact gtgaatataa gaaaccattt tgatgtggat tttctccata tcttaaagtt
    19801 tgttcttttt ggaaagttaa atttaatatg tcaggatacg tatctccaac ttctccagca
    19861 aaataactag atattaacgc atcatatctt cctgtagttg agaaagcttt atatgataat
    19921 ttttttctgt cttctaatgt cattgtatct gtttttaatt tttctattat agaatcataa
    19981 tcttttatat ctactactac tattacatcc ttatagtttt tggcagcaga acgtatcata
    20041 gatggtccac caatatctat attttcaatt atttcatcat gactttttcc agcttttaaa
    20101 gttccttcaa aatcatatag atttacagct actaaatcta ttgaatgtat tttatgttca
    20161 tttacagttt ctacatgaga ttctttatct cttttataaa gtataccacc atgtatatat
    20221 ggatttaatg tttttactct tccatctaat atttctggaa aatttgtaac atcttcaact
    20281 tgcattacat taacaccatt ttcttttaat gttttaaatg tatttcctgt agatataatt
    20341 tcataatcta atttatttaa ctctttagca aattccacta caccagtctt gtcagttaca
    20401 cttattaaag ctctcttact catttataaa cactctccta ccttgaagtt ttaatttatt
    20461 ttcacaaaaa agacttatac tttcttttaa aatcctatgt tcaacttcta acactctttt
    20521 tgcaagtgtc tttgcatcat catcttgatt taccttaact acatcttgca ttatgattgg
    20581 tccagtatca gcaccttcat caacaaaatg aactgtagca ccagttactt tagctccgta
    20641 atctataaca ccttgatgaa ctttttctcc atagaatcca gctccacaaa atgaaggaat
    20701 taatgatgga tgtatgttta tcattttgtt tctaaactca ttaaccagct ttgggctgat
    20761 tatttttaaa taaccagcca aaactactaa atctatttta ttttccttaa gaatttctat
    20821 tatcttgtct tcatcagttt cacaaattgc ttttatgtta tgatttttag ctctctctag
    20881 cccataagca ccttgcttac ttgagataac tactttaacc tgacctttta tttctccaga
    20941 ctcagtacca tctataactg cttgtaaatt tgttccccca ccagatatta aaactcctat
    21001 atttagcata attctacacc ttcatgacta tctactactt caccaattat ataagcttta
    21061 tcttctaata attcactata ttttttatct acataatcag cattgtcatt ttctctatta
    21121 tttataaagt ttacaacatc atcagcattt tctttatcta ctattaaaac tagcccaata
    21181 cccatgttaa aactcttatg aagttctctc tcatcaacag cattaaaacc ttctatcatt
    21241 ttaaatatag gaggcttctc ccaagatttt ttattgatat ctagtcctaa tcctttaggt
    21301 ataactcttg ttatattttc tataagccct ccaccagtta tgtgagctac agcttttatg
    21361 tcatgtttag ctaatacatc taaagctaat ttaacatata ttttagttgg tgttaaaagt
    21421 gcttctccaa ctgtcattcc tagttcttca acatatcgtt ccattttata attgtaagtt
    21481 tctaaaaata tctttcttat aaaagaaaat ccattgctgt gtactccact tgaagatata
    21541 ccaaccaata catctcctga tttaacattg tttccagaaa caatcttttc tttgtctgct
    21601 attcccacag aaaatcctgc taaatcatag tcatcttctc catacattcc aggcatttca
    21661 gcagtttctc caccaattag tgcacaacca gacattttgc aaccatctgc aattccacca
    21721 actatttttt ctatatgctc aggcactaat ttcccaagag ctatatagtc aaggaagaat
    21781 aatggttttg ctccttgaca aattaaatca ttcacgcaca ttgcaactaa atctattcca
    21841 acagtatcat gcttgtccat catctgagct atttttaact tagtcccaac accatctgta
    21901 gatgcaagca aaactggttc tttcatacct acaaaatctt ttaaactgta taatccactg
    21961 aagttaccta aatctccaac tacatttcca tcataagttc cttttatttt accttttata
    22021 aggtctactg ctctatttcc ttcatctata tctacacctg attctttata tgttaacatg
    22081 aaagtagttc ctccttatca attttcttaa ctgggtattc tccatcaaaa caagctttac
    22141 agaaagtaga cttatgttct gttgcatcta acattccttc tatatctagg aattttaaac
    22201 tatcacatcc aatatattct ctcatttctt ctacgttgtt acttgatgca attaatttag
    22261 agcgatttgg agtatctatt ccatagtaac aagagtaagc tactggagga gaagttattc
    22321 taagatgaat ttcttttgca cctgcttctc ttaaagattt tactaattgt ttagatgtag
    22381 ttcctcttac aattgagtcg tctactaaaa ttatagattt tccttttatt attgttgaaa
    22441 gaggatttaa ttttattttt actgctattt ctctttcttc ttgtgtaggt tttataaatg
    22501 ttcttcctac atatctattt ttaactaagc cttcacttat ttttaatcca ctagcatttg
    22561 cataaccaat agctcctggc caacctgaat ctggaactgg aactaccata tctgccttta
    22621 cagtttcatt ctgagctaat ctctcaccac atttaattct aaattcatat gcatttacat
    22681 tgtctatagt tgcatcattt cttgcaaagt atatgtgttc aaatatacaa cttttcttaa
    22741 ctggtttata attttctgaa taaaaatatg attttagttc tccatctttg actactatta
    22801 tctctcctgg ctctacatct cttataactt ctccgccaag tatatctatt gcacaatttt
    22861 ctgatgcaaa tatatattca tcaccctttt taccaagtac aagaggtctg aatccatgtg
    22921 ggtctctaac tgcaaccaat tcttcttgac tcattattac aagagcatat gcacctttta
    22981 tataatccat tgtcactttt aaactttcta ctatatcccc tttgtaatat ctagcaagta
    23041 tatataaaat cacttctgta tctgaattag tttggaacat atatccatct tcttctagca
    23101 tatcttttaa ataatttgca ttaactagat ttccattatg agctatggct aattgtcttt
    23161 ttctacaact tccaactaaa ggttgacaat tagaaacatg acttcctcca gctgttgaat
    23221 atctaacatg tccaatccct atatttcctt ttaacttaga taattcattt tccttaaaaa
    23281 catctccaac taatcccata tcttttttgt acttaatttc tccatcatct aaaagagcta
    23341 aaccacagct ttcttgcccc ctgtgttgca tagaatataa agaataatat agttctttag
    23401 ttacatcttt attagagtaa atccctaaaa ctccacacat acatttgcct cctatttatt
    23461 attcattctt gaaagtactt ccgtataacc ttctacaagg tctccaaggt cttttctaaa
    23521 tctgtcctta tctaactttt catttgtatt tacatcccaa agtctacaag tgtctggtga
    23581 tacttcatct gccaatatta tatttccttc agaatcctta ccaaattcta ttttaaagtc
    23641 aactaacttt aggtttaatt ttaagaaaaa tgcttttagt aactcattta tttttaaagt
    23701 ttcttctctt aagaatttta actcttctct agttgctaac ttcattgcca ctgcataatc
    23761 atcatttagc attgggtcac cataagcatc atttttgtag cttatttcaa aaattggctc
    23821 atcgaaaact acaccttctt caagaccaac tcttttacag atagaaccag cagcaatatt
    23881 tcttactatt acttctaatg gtaaaatctc tactttttta actaacattt ctctatctga
    23941 taaacttttt ataaagtgag tatttatatt attttctttt aacatttcaa agattattgt
    24001 tgatatctta ttatttagta taccttttga agatatttct gctttttttt ctccattaaa
    24061 tgctgttgca tcatctttat aataaacaac atattcattt tcattatctg tagaatatac
    24121 ttgttttgct tttccttcat ataataacat ttctgctacc cccttaaatt attgtcgtct
    24181 tctaacacct tttgtgccat ctcttctcta tatgatttca aatcctcttt taattttggg
    24241 tattttaaag tcattatttg taatgccaat aaagctgcgt ttaaacctaa gtctattgta
    24301 actgttgcaa ctgggattcc ttttggcatt tgaactattg ataaaagtga gtctagtcca
    24361 tccatagttg atgatttaat aggtaatcct attacaggaa ttaatgtttg tgaagctatt
    24421 acacctggta agtgtgctgc ttttcctgct gctgctatta ttacatcagt atcatcttct
    24481 atttctttta ggaatattga aagttgttct ggtgttctat gagctgataa tgctcttgct
    24541 acaacttcta ttccatattt ttcaagcagt tttatccctt cttctaattt tggataatct
    24601 gattttgaac ccataactac tgctactttc attagacttc ccctctccgt ttataagtta
    24661 ttttacacta caaatcattc gtattattat acgaattata caccttaaat tttatattaa
    24721 tatacatata ttatcaactc ttttttgaaa ttttttaaaa aaaatatttc ttgtaatttt
    24781 ttcattcgct acgtatcgaa ctttatttat gtttttttgt taaaataaaa aacagattat
    24841 ctcaaaattg gttttctaat taaattttaa gcccaatact cttttttaaa tcttgtatat
    24901 tattttttat tacttctttt ttaattaata ctttggattt atagtcttgt tttttagttc
    24961 tgaatgttac ttttacttag ataaaaccaa cacaaagttt attctttata taagtaatag
    25021 ctaaaattta aagtaatgat attatataaa tagaaataat tttatttaaa ggagaatctg
    25081 tatgttagaa gttataaaaa atagacgttc aataagaaca tatatagata aaaatataga
    25141 agaagataaa ataactgaaa tactaaaatc agctatgcaa gcaccttcat caaaaaatgc
    25201 tcaaccttgg gaatttatta tagtagatga taaggaactg ctgaaacagc tctcaaagtc
    25261 acagcataga gcaaagcata tagaatttgc accattatgt atagtagtgc ttggaaatag
    25321 agataaattc ttaaaacctg gtaaatggat acaagattta ggtgcttgca cacaaaattt
    25381 attattagaa gttactaatc aaggtctagc tgcttgttgg gcaggagtat ttcctaaaaa
    25441 taaagtggta aataaagtta gacaaacttt agacttacca ttaaaattag ttccttatgc
    25501 acttatatct attggatata gtgaagaaaa aaatgagttt atagaccgat ttgatgaaaa
    25561 taaaatacat agaaatgtat ataaaaatag ataatgttta gtaatataaa tgaaaataca
    25621 tagtaaaaat gcctttagaa tacatttgta ttctaaaggc attagctttg actaaacacc
    25681 taattagcct ctaaggtaaa atttaactat catttattat ttatatacca gttgaatatt
    25741 atatccttca atatttttat ttatttcaaa tatattaaga atatcctaca attaaagtta
    25801 gctatttttc attaaagtat tttattgaca cactccccat agctaaagca aggggattct
    25861 tggtggctaa cacacttatt tatacttaaa cagtacaaca gaggtggtta caaagccaag
    25921 ctacttcgga cggtgtcttc gccactacta ccttaaagtt attcgcccac acatggctca
    25981 aacggtagat acttttaaat ttattggttg cttatgattt attctaaaat ctactaaacc
    26041 atatttatgc tttttattta aaatattcca agcaccaact atatctctat gagtttcata
    26101 accacaagta caactataag ttctaccagt tggtttgtgt tttttagaac actttgggca
    26161 agtttgagaa gtgtagtttt ctttaactaa cttaacttca ataccttgct cttttgcttt
    26221 atattttata tagtccttta ttcgaccata ctccatttga gatattttct gtctattaac
    26281 tctgtttaat cttttctttt tcttagtatc tttttcaacc ccttttatat ccccaagaac
    26341 tatttcacat accttctgct ctacacaaaa gtctataaac tttctagttg tttgatgata
    26401 taaattcatt aactgattat ctgttttact tcttatctta ttttttaatc tccataactt
    26461 ctttgattgt ctactacctt ttttacattt acttaatctt ttagatatat aagcatacat
    26521 cttatttcta aattgtttaa tacttctgcc ttctctatta gatagaataa gccctacacc
    26581 tttatttgtt acactagcta tcgaatgtat ttcccctaag tctacaccta cggtattagt
    26641 gcctttggct acaactccat ttaattcatt gtcaaaagtg tagtataaat aatatccatt
    26701 tcgccatact atttcagcag tttttatatt ctcaacatta aaatctaatt ctaaatacct
    26761 gccttttgac attgataatt ttaagttgcc tttagcattt ctattaatag cactagcttt
    26821 aaaaggtatt atataaaatt tcttagtctt gtatggatat tttgctttag tattaccttt
    26881 ttttcttaat tctgatatag tctttctatt tgcatcaaat ttatctgata tagcttgaat
    26941 agtttgacta tgaagattgt acttaccttt gattaacttt tgtatatcat tctttttaat
    27001 ccattgttta tgttcaaaat agtattcttt agataagtta actatgtagt tccaacagtt
    27061 agcactttcc atttgctttt cccttactaa ttcaaattgt gttctagtta ggtttaattt
    27121 cactctatga gtttttatca tttctttgtc ctaacttgtt gattttctat atattgttta
    27181 actacttcta aactcacaga accaacagta cctacaaaat agctattagt ccacatgcta
    27241 ggcatactag actttaaatg tggaaagttc tctcttaata ctctcgaact aaagcctttt
    27301 aaacctttta ctactttatg aattccatat tgagggtcac aagatattaa taagtgcaca
    27361 tgttctttat cagtttccat ttctattatt tctgctccaa gttcatctgc tttatatggt
    27421 agtatttctt ttaacatctt ttcaacttcg ccaactaaaa caggtttacg atacttaggg
    27481 caaaatacaa tatgatattt gcaatcataa actatatttt tgttagattt atacataaaa
    27541 actaacacct cctaaatata gggtatcagt agcggtatta gattacaagc attattctaa
    27601 ctttttttat attaagacct tacctttcat ccctacacct aaaggagagg gcttttcggc
    27661 aattttattg taattatata tcatttatat aaacaagtac atattttatt ttgaaactac
    27721 aaaattattg tactatattg ttttaagttg ttttataata gattatatca tcttattata
    27781 ggagaaaaaa tatgatttgt actattaaac cattaaagat ttcaataaaa aagtctactg
    27841 aacctctaat tgaatttata tctgatacta cctataaaaa taagagttct tcatatatta
    27901 aagaagtcta ctttacaaga tatataggtg ttgatgattc aattgataaa tacatagaaa
    27961 aaattaaaaa tatagatact tattttttta ataatcctac aattccctat attagattaa
    28021 acaatctaaa aataaatttt gacaaagaac aaacagataa gatgcttaaa atttttaata
    28081 tttataatag ttctaagttt aacagttggg atttatataa tatcgacttt ccatgtaaaa
    28141 tagaaaatga caccttaaat tggactaaaa agattgcatt taaaaatact ttagatttat
    28201 tttccacaag tatgccaaat tgtacatcct ctatcatcaa aaactttgca gttaaacttt
    28261 tgtgttggat agactatttt ctacctaaat tattttataa caaggtgaag actactattt
    28321 caccaaagat agtatacttt ggaaatatta aaaggcaaga actttttttc ttatattttt
    28381 tatcacaact tgggtgtgat attttatata taaatccaaa taaagatatt cttgatttat
    28441 atcctgaatg ttcaaaattt tctactctat ttgaactttc taggaagact ccaaatatat
    28501 tagaaatacc ttttgaaagg catattacaa atataaatta cagtgaaaat accaataaga
    28561 cttctcctac tacgaataaa cctaactcta aaaatacact taataacaca aatctaacat
    28621 ctaaaaaaat tgtacaagat gaggaagtag aactttctta tgaagacctt gcaaagttat
    28681 cagcttctgt tgttatgatt gttgtgtgca ataacgaaaa taaaccgttt aagagcggtt
    28741 ctggagtagt aataaataat gaaggttata tacttacgaa tcttcatgta gtcaatgatg
    28801 ggtattcatt tttagttaga tttgaaaatg atgataaagt ttatacatct taccaaatta
    28861 taaaatacca ttcagattat gatttagctg tgataaaggt tgatagaaaa tgcaagccaa
    28921 tacctgttaa agtcagtaaa aaacctgtga gaggtcaaaa aatagttgca attggaagcc
    28981 ctctaggtct ttttaataca gtttcagatg gaattatttc agcttttcgt gattttgaaa
    29041 cggttcaaat gatacaattt acagctccaa tctctagtgg tagttcagga ggagctcttt
    29101 tagatatgtt tggaaactta ctaggattga tttccgctgg atatgatgat ggacaaaact
    29161 taaatttagc tgttgaaagt agtcttgtca aaatatttgc caataatttt attgagatag
    29221 taaactaatc aatcaaaatt ctacttatcc tattccataa taatcaagag ggtgaaaaca
    29281 ttgcatgctt ttgccctctg attaatattg aaaaaagtat taatactcga catttttatg
    29341 ctcaataaat aattgatgcc atagacaaaa tgtaaatata gtccatattt ttctcgaatt
    29401 atctctatga cctttaagat gcgtatctaa aagttttatt gcatattttt tatctataaa
    29461 ttcatctaca tttgaattat aaatagtttc ctttacaata tcccctagag aatcttttaa
    29521 ccaaactctt attggagttg gaaatcctaa ttttttcctt tgaacaacat gttctgggac
    29581 tatttctcta aacgcctctc taagtaaaac ttttgtatta ttcttattta ttttttgctc
    29641 cattcttaaa ttttttgcga catcaaatac atctttatat aaaaatggta ctctaagttc
    29701 aacctgctca gctgtagaaa gtttgctaat tttttgaagt atatcgcctt gaagccatgt
    29761 attcacatct atatgttgca ttttacttat ataatcgtaa ttattttttt gtgcatctct
    29821 atacaaatta gacaacaggt attcatatat attcttttcc ttatacttaa aaaagaatct
    29881 ttttacttcg ctgttttcaa aaacttttgc atttcctata tatctttttt ctaatggtgt
    29941 tgtagctcta taaagataat ttcttccttt tatgtttggc ataagttctg aaactcttcc
    30001 taatatactt ttaatataag taggtgaatt gacaactgat ttcatagtac tatactcttt
    30061 atatatatta tatcctccaa ataattcatc tgctccttct ccagacaatg ctactttaac
    30121 atgtttacct gcctctttaa ttaagaaata aagacctact tctgaagggt ctgctatagg
    30181 gtcatccaaa gaataaatta cattcggtaa tgattttata tattcatctt gtgttatatt
    30241 aatctggata ttttctatac ctaacttatc agctgttttc tttgctactt ctaattcact
    30301 atatcctttg acactaaagc ctgcagaaaa agattttata tttggattaa tttgtgaagc
    30361 cacagtagtt ataatagatg aatctattcc accagacaaa aatgttccaa tttctttctc
    30421 ctcttccata tgagttacta ttgaatctac tactatattt cttatatcat cctcattcac
    30481 atctctattg cctaagtgat ttttagtata aaaaaatttt gtactacttc taaactcaag
    30541 tttattatat ctctttaaag atagtatacc attttctacc ctaaaatagt gacctgctgg
    30601 tatcagcctt attccttgta tcatagtatc ttcaggaagt acatattgaa atgaaaaata
    30661 actttgtaag cttctttcat ttatatttaa atttttcata tattcaagta aaaacttata
    30721 ctctgaggaa aaaattattg cattttcctt atttatataa taaagtggtt ttatcccaaa
    30781 taaatctctt gctccaaata tactttcatt ttctctatca tagattataa agttgaacat
    30841 tcctttaatc ttatgtacac aattcttacc aaattctata taagatgcta ataagacttc
    30901 aatatcatca tttgtagtaa aattgtgtcc ttttttaatt aattcttctt ttatttcatg
    30961 atagttatat atatttccat taaaaacaat aacttttcca tctttttcaa atggtttatt
    31021 tccataaata gcatcaatta cattaaatct ttcaaaatca aaagtttttt cctcatcctt
    31081 atactctatt ctactagaaa gtcttgtatt atttacagat tcttttattt taaatttaat
    31141 atcttctttt ttagatatat cacctttata ataaattgaa atattgccgt ccatataatc
    31201 aaaattcctt tctgtccttt agtcttattc tatatttcat agttttgtct ttggctttaa
    31261 aacttattca atttaaaatg aaattgctat taaattctta taaattatct gttaatattt
    31321 catatttttt ttatttctag aaatactata ttaggaggtg atttttatga agaaaaaaat
    31381 attaatacca gttattatgt ctttatttat aatctcacag tgcataactt catttgcttt
    31441 tacacctgaa aataacaaat ttaaggttaa accattacct tatgcatatg atgcacttga
    31501 accttatata gataaagaaa caatgaaact gcatcatgat aagcattatc aagcttatgt
    31561 tgataaatta aatgctgctc ttgaaaaata tcctgagctt tataattatt ctttatgtga
    31621 attattgcaa aatttagatt ctttacctaa agatattgct acaactgtaa gaaataatgc
    31681 aggtggagct tataatcata aattcttttt tgatataatg acgccagaaa aaaccatacc
    31741 ttctgaatct ttaaaagaag ctattgatag agactttggt tcttttgaaa aatttaagca
    31801 agagttccaa aaatctgctt tagatgtctt tggttctggt tgggcttggc ttgtagctac
    31861 taaagatggc aaattatcta ttatgactac tccaaatcag gatagccctg taagtaaaaa
    31921 cctaactcct ataataggac ttgatgtttg ggagcatgct tactatttaa aatatcaaaa
    31981 tagaagaaat gaatacattg acaactggtt taatgtagta aattggaatg gtgctttaga
    32041 aaattacaaa aatttaaaat ctcaagatta attaaatatt aaaatccttt tgaaaaaaac
    32101 ttatgtttgc tatataatat aataaaagat agtctaaatt taccaatata atgtttatag
    32161 tgacgattca ggaggatttt tatatgataa aagaaacaca tgaaagggga ggttatatat
    32221 tcgcactttt gatactcccc tttataaaca atctatacct agttaaatac gatattgcat
    32281 ataaaatagt attaattata atttatattt attttgcata tttaggttct ttatttccag
    32341 atatagatat gagaggttct tacataagta aaaaatttat gttaatatac aaattatttg
    32401 gaagtaggtt tagacataga gggtttactc acagtctaat tgccttatta cttatttata
    32461 gtttttttaa atctctgaca atcttcacta acaataatat agttttctca tgtttgtcca
    32521 gtgggtttat aataggatat ctatctcact tgtgtcttga ccttattact aaagaaggta
    32581 ttgaattgtt ttatcccatt accataaaca tttctttact acctataaaa actaactcaa
    32641 aaacagaaaa attcatatct aagctattaa atttcatagt tatttttttg attggatatc
    32701 aattttatat tttattatag ttgtagaaaa aataaataac aatattttga actaagtata
    32761 aataagtttt atatatttta gtttacaaaa gaactaaaaa ttttgtacct taatacaaaa
    32821 aataaaacgt atcttaaaat aatttagtac taaatcattt taagatacgt tttatttcgt
    32881 acaagctaaa tcattttatc atctttatca ttgtgatgtt ttttatgatg gaaatcataa
    32941 ttatgatttt caagattttt atttactcta tttccaatct tccatgacaa aaacaccaat
    33001 aatataaatg atgctatctt taaaggactt gttataaaac cttctatatc acttgccacg
    33061 taacttataa ctataaacat tacaaatttt cctgccaaca ttgctggcac aaaatctttt
    33121 aaatcttttt taccaaaagc tgatgcgata gtaactaaac aaccgggcat aaatggacag
    33181 gcatacgcta taaataataa cttaaatccc tgtttatcca tccaacttat tgctttttca
    33241 gttttactat ttctcaactt attaaaaagc ttactatcat taaatctgct tattaataag
    33301 aataatgaag ttgtcccaag tcctgagcca atccatgata acaaacatcc aataaataat
    33361 ccttgtattg cagcatttgc tgtaactata gcaacaagtg gtaaggctgg tataaagctt
    33421 tctataaatg cacttaataa gccgattatt gtggctaaca cccaataatt ttgagtcaaa
    33481 tcaagtagtt cttgtatata ctgcatattg cttttcccct tttttaactt taactattat
    33541 tatatactct atatgtttat tatataccaa tctcaaaaaa tgaatctaat attaaaaagt
    33601 aaataatcta tataagttta cagttaagg
//
LOCUS       DAELTA010000026        32572 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.026, whole genome shotgun sequence.
ACCESSION   DAELTA010000026 DAELTA010000000
VERSION     DAELTA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.026"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(53..1162)
                     /locus_tag="KPG13_001059"
     CDS             complement(53..1162)
                     /locus_tag="KPG13_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0984799.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            complement(1354..2706)
                     /locus_tag="KPG13_001060"
     CDS             complement(1354..2706)
                     /locus_tag="KPG13_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0984800.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            complement(2905..4233)
                     /gene="ssnA"
                     /locus_tag="KPG13_001061"
     CDS             complement(2905..4233)
                     /gene="ssnA"
                     /locus_tag="KPG13_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG0984801.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            complement(4261..5844)
                     /locus_tag="KPG13_001062"
     CDS             complement(4261..5844)
                     /locus_tag="KPG13_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0984802.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            complement(5860..7047)
                     /locus_tag="KPG13_001063"
     CDS             complement(5860..7047)
                     /locus_tag="KPG13_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0984803.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            complement(7377..8591)
                     /gene="dpaL"
                     /locus_tag="KPG13_001064"
     CDS             complement(7377..8591)
                     /gene="dpaL"
                     /locus_tag="KPG13_001064"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0984804.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(9214..9753)
                     /locus_tag="KPG13_001065"
                     /pseudo
     CDS             complement(9214..9753)
                     /locus_tag="KPG13_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(9830..11635)
                     /locus_tag="KPG13_001066"
     CDS             complement(9830..11635)
                     /locus_tag="KPG13_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984805.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(12031..12408)
                     /locus_tag="KPG13_001067"
     CDS             complement(12031..12408)
                     /locus_tag="KPG13_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0984806.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            13313..13798
                     /locus_tag="KPG13_001068"
     CDS             13313..13798
                     /locus_tag="KPG13_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984807.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(13998..14537)
                     /locus_tag="KPG13_001069"
     CDS             complement(13998..14537)
                     /locus_tag="KPG13_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0984808.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(14530..14943)
                     /locus_tag="KPG13_001070"
     CDS             complement(14530..14943)
                     /locus_tag="KPG13_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0984809.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(14979..16100)
                     /locus_tag="KPG13_001071"
     CDS             complement(14979..16100)
                     /locus_tag="KPG13_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0984810.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            16688..18340
                     /locus_tag="KPG13_001072"
     CDS             16688..18340
                     /locus_tag="KPG13_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG0984811.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            18639..19388
                     /locus_tag="KPG13_001073"
     CDS             18639..19388
                     /locus_tag="KPG13_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984812.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            19401..21428
                     /locus_tag="KPG13_001074"
     CDS             19401..21428
                     /locus_tag="KPG13_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984813.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            21572..23155
                     /locus_tag="KPG13_001075"
     CDS             21572..23155
                     /locus_tag="KPG13_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0984814.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            23127..23747
                     /locus_tag="KPG13_001076"
     CDS             23127..23747
                     /locus_tag="KPG13_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0984815.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(23936..24862)
                     /locus_tag="KPG13_001077"
     CDS             complement(23936..24862)
                     /locus_tag="KPG13_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0984816.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            25492..26412
                     /locus_tag="KPG13_001078"
     CDS             25492..26412
                     /locus_tag="KPG13_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0984817.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            26498..28918
                     /locus_tag="KPG13_001079"
     CDS             26498..28918
                     /locus_tag="KPG13_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0984818.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            28918..29940
                     /locus_tag="KPG13_001080"
     CDS             28918..29940
                     /locus_tag="KPG13_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG0984819.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            30055..30801
                     /locus_tag="KPG13_001081"
     CDS             30055..30801
                     /locus_tag="KPG13_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0984820.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            30887..32137
                     /gene="cme"
                     /locus_tag="KPG13_001082"
     CDS             30887..32137
                     /gene="cme"
                     /locus_tag="KPG13_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG0984821.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
BASE COUNT        10979 a         5304 c         4198 g        12091 t
ORIGIN      
        1 attatcctaa tacctattac tctaagttaa ttaccaaaat tctatataat tattactcat
       61 tcatagcttc tacttgattg tagcagatgc ttaaagctcc ttttggacat ctagttacac
      121 ataatccaca tccaaaacac ttgtctgcat ctatgttcaa cttatctgta acttctaaag
      181 cttcgtatac acagcttgtt acacattgct tacatttgat acacttgtta tcatccacta
      241 ctggagctac tgaatgagtt ctaaaccctc tagctgccat tttcttatga gttagtcctt
      301 ttatttcatt aacatcagca tatccattct catctaaaaa ttcatttaat tctttagcaa
      361 tttttccata gatagaaggc cctttaagga ttgcttctgt acatacttga acagcacttg
      421 ctccagccat catcatttca gccacgtctt ttccacaagt gattccacca acaccgatta
      481 ttggtatatt tacaacttga gaagcttcat atatacatct tattgctaga ggtcttattg
      541 gtgcacctga taaccatccg tatccagctt tacttcccat tatagggtat cctgtattta
      601 catctattgc catacatggt ccaaatgagt ttatcattac aagtccatct gcacctgctt
      661 cttcaacagc ttttgcaata gtttgaatgt cagtatgagg actcattttc ataaatactg
      721 gtacatctaa aacagctttt gcagctttta aagcatttac tattggtgca acgtctgtac
      781 ctacataatg agtagataat tcaactgcat ctgcataagg ctttactaaa ggagcaacct
      841 cagctatttg ctctgctgaa tatcccatac ttactattac tggaagccct gtttctttta
      901 cctttttgta ttcttcctct atccattgct cttttggaag ctctgaccat aattctgtgt
      961 ttaaaaaacc actattagtt tttgccatac atggtcttgg tacatcagct ggtaatacag
     1021 atatagtttt tgtacatata gctcctgctc caccttttgc tgcctcttga cagagagcac
     1081 catcttttgc tccaggtcct gccgctgtca taattggatt taaaaattcc attccatata
     1141 aattaacttt taaattagcc atattattat ctctccctta aattaaatat ttttaatatt
     1201 tagattgtat tcttttattt gtatttgtta tttatttact tttttatatt atttaaaata
     1261 taagatagtt ttgttgaatt attaatatct ctgaattatt tttattaata cttaatgact
     1321 ttaagtcaat atagattttt acttgtttta tttttatttt gcttcttcaa ctttattatt
     1381 tttttctttt ttagaaccta gttcattgaa gaatatgttt aaaagcactg ctgataaact
     1441 acctgttgtt attccactat ggaatattgt ttgtacccaa gttgggaaat tatgatagaa
     1501 atcaggattt gttagtggta tcatagacaa accaacacta actgctacta taaccccatt
     1561 tttagttcca tcaaatttca ctttgcttaa agtttttact cctccaacca ttaccattcc
     1621 aaacattgca aatcctacgc ctccaagaac aggcttaggt attgctccta ttattgctgc
     1681 aaattttggt aagaatccaa gtactagcaa tataacccct gcgtaaactg ctataaatct
     1741 tgattttata cctgtaagac ctactattcc tgtattttga ccaaatggag ttattggaaa
     1801 tgaattgaat attccagata atgctgtaga tattccatct ccaaatagac ctcttttaat
     1861 atttttacct gttacttctt tgtctaccat ttcatgaata gctatcatat taccaactga
     1921 ttccgtcata agtaccaaca taatcaaaca caatgatagt attgcagatg gttcaaatct
     1981 aggcattcca aattttaaag gtacatttaa gttaagccaa ccaactccat ttactgcact
     2041 aaaatctgcc attccaactg ccatggaaag tagtgtacca agtactatac ctaccaatat
     2101 agctgtgcta ttccaaattc cttttaagaa tttaaatgaa agtaaagtta ttgccaatac
     2161 tgctaaagct aatgctaatg agtttggttc aaccgcttcc aatttgttgt cagttatcca
     2221 ttttatccca actggaaata gtgaaatacc tattaccata acaactgttc cagtaactac
     2281 actagggaaa aatcttatta atttttcaaa tactggagca agtaatatac aaaacaatcc
     2341 agctactatt acagagccaa atactgtctg tattgcaact tgtgaatcac tatatgatga
     2401 ggctattgct gtcatacctg ccacagcggc aaaactagtt ccttctatta ctggtatttt
     2461 agcacctaca atatttccta taccaacact ttgtatgagt gttcctatac ctgccataaa
     2521 caagcaagca tttattaaaa aaacaatact agcatgatct agtcgtgcag cacttcctat
     2581 catcatagga actgctactg ctcctgcaca cattgctaaa acgtgctgta gtgaaaatac
     2641 ccaagatttg gatacaggta acctttcatc tactggagct attttagggc ttttatctcg
     2701 agacattttg ggtctccctt ctatatttaa tttaatattc tattccgtat attttttatt
     2761 tatatacttc atttaaattc ctatttttgt ttaagctctg tttttaacat ctaaattctt
     2821 gtttcttact tgtatattaa tctgaacctc ccatgactaa agtcacaggg ttcctgcttc
     2881 atagaatttt gcataatcaa ttatttaaat tctattccat agattttgac ttaattctct
     2941 actttttgct agaatacttt cttcatctat gtttattaac tttctatctt tcataagtac
     3001 ctttccattt ataatagttg tatctacgct tcttccactc attccaaaaa tagtgtgtcc
     3061 ccctattgta ttttctgtca atggagtatg tggtatataa tcaactacta taacatctgc
     3121 ataagcacct tttttaagta ctcctaggtc atttttataa taagctttag ctatttttct
     3181 attgttatca aataacattg tttgtgattc ttcaaatcca actcttgggt cttttaagtg
     3241 atgtttgtga attatattgg caacttttaa tgattcaaac atatcgctag tatatccatc
     3301 agttcctaat cctactgtaa ctcccctttt taataactct attgcaggag aacatccaac
     3361 tgcatttccc atattagatt ctggattatg aactacaaat gtatttgttt tatttaaaag
     3421 actgatttct tctgcactta tatatataca atgaactgct attgtttttt cttttagtac
     3481 tccaaaatca ttgaatcttt ctaaaaccct ttttccatat ttatctacat tatattgtaa
     3541 gtctgctagt ccttcagcaa catgtacatg gtaacctgta tcaagaccag ccatctctga
     3601 agcacattta tttaatgtat catcagatag tgtaaatgca gcatgaagcc caaacattcc
     3661 ttttatcatg tctgtatcat ctttttgagc gtattttata aagtttacgt tttcattgat
     3721 accttgttgg attgtatttc caccatctct atcagacact tcataacaaa gacacgttct
     3781 aattcccaaa tcctttgcaa catctgatat tgtaaacaaa ctgccttcaa ttgcatttgg
     3841 acttgcatga tggtcataaa ctgttgttac accatttttt atacattcta tatacgttgc
     3901 atatgcactg tattttgtat cttccagtgt aagctgtttg tcaagtctcc accataaatt
     3961 gtttagtatc tctaaaaagt tctcagaagg ttttcctgga actgccattc ctctagcaaa
     4021 tgagctgtat atatgcatat gtgaatttat tagacccggc atgattattt tcttgcctgc
     4081 atctataaat tcatgtactg gatatttagc tttcatttct tctgtagtac ctatatcttc
     4141 aattagatta tcatttatta ctacacatcc atcttccata tagggattta aagaatcttg
     4201 gcttattagc ctcccattcc ccactaaaat cataaattta ttcctcctta aagctaagat
     4261 ttaattttta agtacttttc ctgctttaat tttattgtat tttccttctt caacaacaca
     4321 atttccattt ataattacgt aatctatacc tgtagggtac tgcatagagt tgataaaatc
     4381 atcacaacca ctaactgtat ccttgtcaaa tataactata tctgcaaaat atccttcttt
     4441 taattcgcct ctatctttta tactaaagct tgtagctgcc tttttagtca ttttataaac
     4501 agcttcttcg attgttaaca cattcaactc tctaacatat cttcctagta ttttagggaa
     4561 agaaccataa gctcttggat gtggtttacc tgctaaaaga ccatctgtac acacattttg
     4621 ctcgtctctt ttcatgaatc ctattatatg ttcttctaat ccatagaaat ctaccatccc
     4681 aactgcattt tcttcttctt ttaataaatc aaacgttgca tctaatgggt cttttcctct
     4741 catttttcca atttctaata agttcttacc aattgtatct ttattctttt ctgtttttac
     4801 gcttgttaca aatatttggt ctattccagc aaattctata aaattatccc agccttctat
     4861 tccatttgct atatcttttt tcatttttgc tctttcgttt tcatcactta atctttctat
     4921 taacttatca gttcctccag aatgtgccca aggaggtaaa actactccaa gcattgtact
     4981 tcctgctgca tatggatatt ggtcaaaaga tactcttatt ccttcctttt ctgctttttc
     5041 taaaagctct ataacttgtg gtatatactt ccagtttgct ttaccacaaa ccttaaaatg
     5101 tgaaaaatga acttttactc ctgattgttt tccaatttct atgatttctt ccattgaagt
     5161 taaaattgta tctgcttcac ttctttggtg aactacaaat actccatcat attttgctac
     5221 taccttacac atctctataa tttcttcagt caatgagtat gcacaaggta tatatataag
     5281 tccagtagac aatcctatag ctcctgcttt taattctctc tctgtgattt cacacatttt
     5341 ttgaatttct tctttagtag caggtctatc ttcaagtccc atagcttcca ttctaacgtt
     5401 tccatgaggt actaaataag tttcatttaa acctacccca ttgtaatcca tcatttttaa
     5461 ataattctct gttgtttcat atttccaatc aatttcatca ctttcaccat ctagacctgc
     5521 taaatttttt ctccaagaag atatatgctc ttgaggtaga ggggccatag atataccatc
     5581 ttggcccaac acttctgttg taattccctg tctaatcttt acctcattat atggattaac
     5641 taaaataact aggtctgaat ggctatgagt atctatgaaa cctggacata taactctacc
     5701 ttttgcatct ataactttgt cagcttcttc gtttaaatct tgtgatattt ttaatatttt
     5761 ctcgttttct attaaaatat ctccttcata tgctggtgtt ttatttccat ctactataag
     5821 cccatgtttt attaaaattc tcacaaaacc atctcctaat tattttttat aaactgattt
     5881 taatatacca taatatcctt ctgcaccttt gaataattgg tctaattcta tatactcatc
     5941 tattgtatga gcaagatttt ccttagaagg accaaatcct atagttctta ttccagcctc
     6001 acctgcgtag tgacttccat tcgtacagaa tgagtattga gttatttctg aatctattcc
     6061 agcttctttt aatcccttat acgctgcttg aacaaattca tcttcttcat catatagcca
     6121 acctgggaag aatctttcac tttctatagt atttcctgta tagcaatcag ctttttcaac
     6181 tgcataagaa acttttgcat ttaattctgg gtcttctttc atcatttcat ctaataaagc
     6241 ttgtattgga gctaatacgc cttctcttgt ttctccaact aataatcttc tatcaaaagt
     6301 agctttgcaa taatctggca ctactgatgc tcctggatat ggagaagatt ttatatctgt
     6361 taaaactaat attccatcac ctaaaactgg atgagttgga ggtactaatt gttgtatctt
     6421 ttggattaca ttagccattt tataaactgc atttatacct ttttctggat tagcagagtg
     6481 agctggttta ccaaaagttt ctactactat ttctcctcta cctctttgac ctatctttag
     6541 gtttaattct gaagattcac ctattacaac ataatcaggt tgaacagcct tacttatctc
     6601 tctagctgaa acaccttcaa atatttcttc atgaacaaca cctgctacat acaattcacc
     6661 ttcaaagtct ttgttagtat cttctgcaaa gtatgaaaca gcagccatca tagcacttac
     6721 ttgccccttc atatcagaag ttcctctacc atatattttc ccatctacta ttctcgcttc
     6781 aaaaggagga acactccatt tacttgcatc tggaactgga actgtatcta tatgcccgtc
     6841 aaataatatt tttttacctg gtttatttcc ttttatacat cctactatat ttccatatct
     6901 atctcttgac caactatcaa aacctagtaa agcaaaattt tcttctatag cttttactac
     6961 gttttcttct tgacctgaat aactagcatt tcttattaag tcttggcata cttcagttaa
     7021 ttgttttttt cttgtttcat ttaacatttt gatattcccc catataattt ttaatttgtt
     7081 gacgttttat tttgatattt cattttttta atttattagc atttcactta atctattgat
     7141 tttttatttc tttatatttt atttattaaa tttttattta gactttattt taattttatt
     7201 tggtttttat tttgaatttt tcatttgctt tactttttta tttgctttac tttttcattt
     7261 atttagattt ttcatttgtt ttagttttta ttttatttaa agttttcact tatttaagtt
     7321 tttattttcc ttaaattttt catttatttg ttaagatttt cttttcctta agtcttttat
     7381 ttactttggc attcaccatt ccaaactata tctctatatt tatcagggtc agtatctcct
     7441 tctgtactta ttaataatac tttagagttc tcatctagtt tcaaagcttc tcttaagtct
     7501 tttaaatctt cactttcaag gattgaaact aatgctccag ttgtaactgc tccagattct
     7561 ccagagatta ctttctcatc tcctcttaga gggtttccta atattctcat acctttagca
     7621 gatacccagt ctggtgctga aacgaatgct gatgaataat tttttaacac ttcaaaacct
     7681 atagtgtttg gttctccaca agcaagccct gccattatag ttggcatatc tcctccaaca
     7741 aatcttggtt ttccatctcc agcttctgct gatttatagt aacagtcagc ctcatcagcc
     7801 tctactacta ctgttattgg gcattcatca tatattgaag caacatatcc ttgaactgca
     7861 ccagcaagtg aaccaactcc tgcttgaaca aatacgtgtg ttggtctatc tactccatat
     7921 tcttttaatt gttctatagc ttctttagcc atagttccat atccttgcat tatccatgct
     7981 ggtatttctt catatccgtc ccatgctgta tcttgaacca ttactccatg gtcatcagct
     8041 tctgcataat cattagctaa tcttactgca tcatcatagt ttaagtctgt tatactagca
     8101 tctgcacctt ctcctttgat tttatttaat ctaaattcag aagaaccttt tggcattaat
     8161 acaacagatt tttgacctaa tttattagct gtccaagcaa ctcctctacc atgatttcca
     8221 tctgtagctg taaagaaagt tatttctcct aatttttctt ttatttcttt tgaagttaat
     8281 ttatcatacc ctaactcaga tatatctgta tctaatcttt gagctaagta tctacccata
     8341 gagtaagaac cacctaaaac tttaaatgcg tttaaaccaa atctataaga ttcgtcttta
     8401 acatatattc cgcttacgcc tagtgatttt gctaagttat ctaatttaac taaaggagtt
     8461 ttttcatatt gtggaaagct ttcatgaaaa gctttagcct ttgcaatctc ttcatctttt
     8521 aaaaattcaa ttcctttttc tttttcctct gatttaggta agttatttaa tttccatttt
     8581 atttctctca tttattttat attccccttt caaatttttc ggtgtcattt taatttttta
     8641 tctttgtgcc ttatagcaaa gcaattaccg tgccaatatt atttattcta aaaacaattt
     8701 ttttatgttg gaattacata cttttataaa atttctttaa agtttataaa ttttgagtgt
     8761 gaaaaataag atttataaaa gaaaaaattc tcatattggg aaatttttat caatacgaga
     8821 attatcaatt cgagaaatcg tttatttatc ttaattcttc ctttattatg ctcttatttt
     8881 ttatatgtct attgttttgt cagatatatt actactttta ttttctataa atatatggct
     8941 ataaccttct tacgcataaa ctctataaat ttatatactt tatattcttt attacccaaa
     9001 tattctttat tacccaaagc ctccaaaatg gcttaaatct tttcatagct tcttacagaa
     9061 gtttacatgg gcgtcccccc aataagcaaa agtgcaatat gtgtaccatt gtgccctact
     9121 tgcgactatt gagctgtcga aattcgcaac ttcgcattta tctgatgaaa tgcactatac
     9181 aaaatattca taacatccac aacagagtta aagttaccta ttcatattca ctgtaaaaat
     9241 ttcttccaca ggaaatgtaa ttttaaccaa tcctattgag acaaactcac cacttatttt
     9301 tttgaactca cgcagtaata catcacttgc ccgaaaaaaa gcctcacttt catcatctcc
     9361 agtaagtgct attgtgcaat ggggtaccca acgattgggg tggtaccatt cccaaccatt
     9421 tgaatcaaaa tcttgtagta cctcatataa ttctctctga aactgctaca tactgctatt
     9481 cattataggt gatacaaaca ccgttttagt atcattaaac atgcctactg aaccaatgta
     9541 agcaggtatt accttatggt tttgtgcaaa cccctttagc ttgtctatac aacaagcctc
     9601 atccacatca ttgaaacaag ccaaagtaag atgaggtctt gttttccatt ctaaatactt
     9661 cgtactaatt ttttcatcag cgattttttt agataaatca aaaagttgct tttccgtttt
     9721 tttatcataa tacagctcta tcgcatattg cataataaca cctccaccat aaagtaaaaa
     9781 tacctatcta taaaaacaca caaataccta tcattttaat attatattac tactgttcct
     9841 cttccaattt tctatatagt gtagccaaac ctatgcctaa gtcttttgct atcttcttct
     9901 tcgtctttgt atctcttcca tacttattca acaacttgtt tatgtaagtc aactctaatt
     9961 ctttaattga aagtatcctt tcttgattct ctactatccc tccaactata tcatcttcaa
    10021 atattatatt tacatcctta tttttataaa tatttccatt aatattataa tagtttaaaa
    10081 tatctaatgg aagcatatct ttatatatta ttccatcatc tccaaccaaa ttcatcatat
    10141 attcaactgc attttccagt tcccttatat ttcccggcca attatagtta atcaacatat
    10201 tcattacatt ttcttctatt ttatttaact gaatacctaa ttctaaagaa tatttagaag
    10261 caaacttcat cattattgcc tcaatatcat cctttctttc cctaagaggt ggcaaattta
    10321 taggaattac attcaatcta tagtataaat catccctaaa cttaccttca tttactaatt
    10381 tcaataagtt tttatttgta gcagcaatca ctcttatatc taaatctata agtttatttg
    10441 acccaattct cgcaaatttt ctctcttgta aaactctaag taactttgct tgtaaaggca
    10501 ttgacaaatc tcctatttca tcaagaaata taacaccttc atttgcaagt tcaaatttac
    10561 ctactttacc tccactacta gctccactaa atgcaccttt tatatagcca aatagctcac
    10621 tttcaaggag attttctgga atagcagcac agtttatcgc aataaatggc ttgttccatc
    10681 tgctaccttc agcatgtata gccctagcaa ttagctcttt acccgtacca ctttccccag
    10741 ttataagtac agttgaattt gactttgcta atttcttagt tctctctttt acttttaaca
    10801 ttgcttctga atttccaatt atagactcta tatcactatt tccccagcca ctattcactt
    10861 ttccatgtcc tttatgattg atatatgtct tgtcaaatat tattattttg tcgtattgat
    10921 ttatatatgg atataccgga attattttgg catttatatt ataaattttg ttatcaatat
    10981 caatttctag taattcatgt ccatttgagg aagattcaac agatactata tttactatat
    11041 tttctattat atttttatct atatctaatt tttttagagc tatattgttt atataagata
    11101 ttttgctatt tatgtctagt attataacgc ctttttcaac attttcaatg atttctatca
    11161 tcatatccat ttttctactg ctattttttg acgcttcatg ttcttctatt ttcatagaaa
    11221 ttaaatcgca tatatgattg acaaatttta aataattatc tatttttgca cttatttcta
    11281 cttttttagt tttatctgta ctaatagcac caattactcc tatagttcga ttattatact
    11341 ttattggaac tgctattatt actttattta ggcatttcat gtaatgggaa cattctatac
    11401 aaagctgatt atcacatata tccaatacga ctaattcctg acctgtctgt ataacattgt
    11461 cataaataaa gccctcagta acaacgcttt catttatctt tgatttatac agacctgtac
    11521 cactaattct tattaatcta tcgtcaacta tttcaatatc tacatctaac acactagcta
    11581 ctgtttcagt gtattttatc atttcttgtt gaaattcttt taatttactt aacatagttt
    11641 cccccattag aatttaattt accaaattat cagtttagct atattttaac atatagtcac
    11701 tctttaaaat atatataaaa cattacttat ttatatattt gcgttaaaac agacaatatt
    11761 aatttataaa aagtttgttt tatatagtaa ccttaattat aatatgcaga caataaaata
    11821 aatcacaata caataataaa aaaaagcttt cctcatataa caaatatatt tttgacataa
    11881 aaatatacat tatatgagaa aagctcaata gttaaatatt tagatagatt tttatataga
    11941 cttttttaat ataaactttt ttaattgtag acttttttta ttagttttta atttttatta
    12001 gttttgttat tggttttatt gttataatac ttataatact gccattgctt caatttctaa
    12061 aactgcatct tttggtaatt gagcaacttg tacacatgac cttgctggtg gattttcttt
    12121 aaaatatgta gcatatactt cattaatatc attaaaatcc tttaaatctt ttacgaatac
    12181 tgtagtctta aatattttat ccatacttgt accagcttgt tctaatattg cttttacatt
    12241 atctaaagaa gcttttgttg cttcttttgg gtcagatact aactcgccag tcttcatatc
    12301 tattggaagt tgacctgatg tagaaactat gtttccaact tgaattgctt gagaatatgg
    12361 tccaattgct cctggagctt tctctgtagc tattactact tttgacatga cttcacctcc
    12421 tattattaag ttatatttac aggaaaataa aatttcctaa acaccaatat taatacgaac
    12481 ctttagatta aacttcaaat ctaaaacttc aaataatcaa tacttagatg attatgttta
    12541 tttgtgtctt gttaactttt attaaagcaa ttaacgtgcc aaactttatt tatatataaa
    12601 attttgtgaa gtcattcaaa atagttactt ctgaattttt tcgtaaaaaa acgctctaaa
    12661 attacttttt tatgatgtca atttttttct catatctaga aatttaccaa catgagaatt
    12721 cttgttttga gaatttacag attaaagtta ttttttaacc ttaaattctg aacctcccat
    12781 gactaaagtc acagggttcc tgcttcatag aattttgcat aatcaagtta ttacatatga
    12841 aagaactata gttgacaatc ccactattat aaataataaa acctttaccc ataccttatt
    12901 taccctagtt atcatagagc ctattgaaaa tataacagct aaatataata ataaccaaaa
    12961 aattatcaat atctaccact gttgctgaaa atccaaaaaa ctaccttttg atagtattac
    13021 ttaacaatct aattaatctc ataataaatt tacaacttat aaataaattg ttatattggc
    13081 cgtatctcaa caactttctc ctttcctagt tttaaatctt ttatagaatt gtaccacgtc
    13141 taagtaaatt ttcatactat taagtcttat ttctaataaa tcattagctc tcattctatg
    13201 cccaataatg taagattgca gtaattttat tgattttaat agttttattt aatatactta
    13261 tattaattat tttcatatga aattattatt tatatagggg gattttttta atatgagcaa
    13321 aagtgaatta acagcagaaa caacagaaga aatgttagaa gtactaagtg gtaaagatta
    13381 tgatattgca tgtcatttac atgaacttgg taaatcatta gattgtaaaa ttgaaccaaa
    13441 aacaggtgct cgttcttaca aaatagtata ttcaactaag aaaccaaaac gtagcttatt
    13501 tactattgaa tgtaatgaaa aaaaatggag agttaaagca aatctttttc atctaaatac
    13561 atataaagat gctgtggagg aatgctctaa aactattaaa gatagtataa ctaaaactcg
    13621 tacttgtaag aaatgtaatt caaagtgtat tggaggttct tcgtttgaat tagatggaaa
    13681 gtcttacctg acttgcatag gaagtggtca ttattttgca aatatggaag aaatggattg
    13741 gaaaaaccta gaaaaattaa ttactaaaga aaataatatt atgcaggaat ctgtatagtt
    13801 ttttaagggt aattattaaa atatatgagt aacaacagat acttaaatag gcataaaaaa
    13861 ataaagggta taataaggat atagaataaa aataaatgat atagcacaaa gaggctgtct
    13921 caaaatacag ataagcctct ttttattgtt gaaatgataa gaatatattc aattttatac
    13981 aattttatac aaacatacta cgccaactta cccatttcat atgcctgttt cattgcctta
    14041 cttcccttaa tatcacctac attccaagca cctacaccat atactatacc tttttcctta
    14101 gaaccattta gacatgatgt aaatcctcta aatccttcta ttgttctttc catatcagcc
    14161 acattttcat ctgctgctgt aacgataaaa tagaaatctt tatcacaaat ttcagtatat
    14221 ctagagcaag ttctatcaat catagttttc atttgcgcat tcatagtata aaaataaact
    14281 ggagtcgcta aaacaatcac atctgcctca atcatttttt caagaatttc tcccatgtca
    14341 tctgtctgtg aacaatttgc ttctttttca taacaagcac cacatccagt acaatagtta
    14401 atatctttat cttttaaaaa tatcttctcc acttgatttt tagattctaa taccccttgt
    14461 ataaactggt cacacaataa atcagaattt ccaccttttc taggactagc tgatactatt
    14521 aatacatttt tactcataag atactgcctc cattttctta tttctactgc tctttaatac
    14581 tttttctttc tcaataatac cttcttcata tctatcaatc ttaaaattta atcgttccaa
    14641 agttttttgc atttcttcta ttctttttgc tagttggttt ctctgctcta ttaaaagttc
    14701 ttttctagct ttgatagtac tgctcccttg ttgaaacata gctacatact caattaatgt
    14761 ctcaatagaa agacctgcac ctctcataca ctttatgaaa ttaacccact cacaatcttc
    14821 ttctgtaaaa gacctaattc cacttttatt tctatttaca ggtggtatta aaccaattcg
    14881 ttcataatat cgtaaagtat ccgctgaaag ctcgtatttt ttactaactt ctgaaatcgt
    14941 catacatatc ttctcctctt gatattgtat agattgaatt atttctcaaa ggtttctgct
    15001 acattcttta aagcatctat aattttaatg tgttgaggac agctttcttc acattgttta
    15061 cattcaatac aatctgaggc ctttccataa gttttagtat aattattata gtagaccatc
    15121 tgtgttgaaa atccagtatt aagagcttgt ttttctgcat tgtacaacgc aaaatagttt
    15181 ggaatagcga tattttttgg acagccttct acacaatact gacaggcagt acaaggaact
    15241 aaaattgatt cgttgataat ctctactgct tcatctataa tattatattc ctcttgtacg
    15301 aatggtttga aattttgcat atatcctgta ttgtctaata attgttccat atttgacatc
    15361 ccacttaaga ccatcataac attatcttta cttgctgcaa aacgtattgc ccaagatgaa
    15421 attgacatat ctggattgta gtcattcaat aatttctctg ctttttctgg tatctttgca
    15481 agtgtacctc cttttactgg ctccataaca attacaggct tattatgctt ctctgccacc
    15541 tcgtagcatt ttctagattg tatgctttca ttatcccaat ctaaataatt aatctgtaat
    15601 tgcacgaagt ctacttctgg atgctcactc aagactttat ccaataattc tgcactatca
    15661 tgaaaagaaa aaccaatatt ctttattttt ccttcttttt tcttttcttc aataaactta
    15721 aagctatcaa attttttagc tacttcataa tgtgacacgc ctatattgtg taaaagatag
    15781 tagtcaaaat agtctacacc acatttttct aattgctcat taaaaatggt ttcttgctct
    15841 tcttttttct ttaaagccat taatggaagt tttgtagcta tagtaaaact ctctcttttg
    15901 tgtcttttta ctagagattc tctaagtgct atctcacttt ttcccatgtg atacacatag
    15961 gctgtatcaa aatatgtaaa tcccctttct aaaaaagtgt ctaccatttt atttaaatgt
    16021 tccatatcaa tagttgtaac atcattttca tcttttattg gtaaacgcat taatccaaat
    16081 cctaattttt ttgcctccat tgttttactt ccccctttaa aacttgtttg ttttttctct
    16141 ctactttaac tattcttaca agtatataat tcttgtataa ttatacttta ctacttggag
    16201 ttaacttcaa gtcaatagat ttttttattt ttaagacaaa tattataaaa atacagttct
    16261 ttaatattaa tccattacat gtttctttca ccctccaatt ctcaaacttc acaaactcca
    16321 acatgtaaag aaatatatac tttaagaaat tcagtccatt aatattacta caaatacttt
    16381 ttagatatgt ttaaaaagaa atttcaccta aaagatttag aaataggtct tgtgtctatt
    16441 ttaaaatata taaaacagtg gtaaagactt atttataaga taaaaaatga atagtgtata
    16501 ccctttttaa aaagtttttt cctttttata tttgtgatac aatgttaaat ataaaaagga
    16561 aaattttgta ttaaaaaaat cttatttgtt gattttgtaa cttcaaactc agcatcaatt
    16621 tgattttaag attatatttt attaagtatg ctaaataaca aatttttaaa gattgtgagg
    16681 taaaaatatg aataagaaaa atttagctat attaatatca ggtattatgt ttttttctag
    16741 ttcttcagca atttttgcag acaatactac aaagcaagaa agattaatag gtaaaacaag
    16801 atatgaaaca gcagtagaag taagtaaatt gggatgggtt caatcaaaaa cagccattat
    16861 agttaatggt aattctattc aaagtgcgct ttgtgcaaat ccatttgcta agctaaagaa
    16921 tgcacctata cttttagtaa acaacaacag tattgaaaat tcaactaagg ctgagcttaa
    16981 aaggcttggt gtagataatg tgtacattgt agatagtgga aatagcattt cttctaaagt
    17041 agaaaatgaa ataaaatcac ttaacataaa aataaataaa atagtaggaa acaatatata
    17101 tgaaatgtct acaaatgtac ttaaagaaat agataagata aaaaaggtag aaaatgttgc
    17161 agttgtaaat ggactaaagg gactatctga tgcagttagt atagcttctc cagcagcaat
    17221 acataatatg cctataatat tagtatcaga tgaaaacagc aatgatagta gtataaattt
    17281 cttaaacaat aggtctataa aaaaatctta catagttgga ggaaataaga ttttatcaga
    17341 caatacagta aagaaatatc caaacagaga aagaatcagt ggttcagata gaaacaatac
    17401 taatgcaaaa attattgata aattttataa agataaaaaa ataaataatg tatttgtagc
    17461 taaagatggt attaaccaag aaaatgcatt agtagattca ttatcaatag gagtactagc
    17521 tgctaaagaa aattctccta taatgatatc taatggtagc cttgtaaata gtcaaaaaga
    17581 atttattaaa aataaagata tagatgaaat aacacaagta ggaggaggat ataatcaaaa
    17641 agcatttgaa gatattttaa aactaaaaaa tgtgaacata agaaataaaa ctaaaaaaat
    17701 atctttaata caggctaaag aaatattaaa acaagctctt ccaggaagca atattttagt
    17761 ttgttattat gatgaagatg ataatgaata tgactgcaaa gttataaagg ataatatcct
    17821 atatgagata gaaattgatg cctactctgg taaaataata gaaattgaaa aagatgatga
    17881 cttaaatgat aataatttag gtacacaaaa tatatcattg gataaagcta agtctatagc
    17941 attagatgct gttcctaatg gtaatttaat ctattgtaaa tatgatgctg aagataacga
    18001 atatgaggct aaagtaataa aaaataagaa aacatatgaa ttagagataa gtgcatttga
    18061 tggaagaatt gtagatattg acatggacaa tgattctgat gatgactttg atgataatga
    18121 taagaataac tctaatacta atgacaacag ttctattatt agtgctgaaa aagctaaatc
    18181 aataatgcta gataaagtgc ctggtgggaa aatagtaaaa tttaagtatg atgaagatga
    18241 caaagaatat gaaggtgaaa taataaaagg aaaaactgaa tatgagataa caattaatgc
    18301 aattactggt aaaatattag aatttgaaat agatgattaa taaaaaatat atattctatt
    18361 aattattaca agcaataaag ccataaatat tgaaaatttc aatatttatg gctttatttt
    18421 atttttttta atttaaatcc acattatgcc attatatata cctatgcact cttttaaatt
    18481 aaaatcttgt ccatcatccc aaacctaata aagttagatt ttaaacaata tattgtgata
    18541 taagtaattt gtgtagaatt attgcgtgat ttactcatga agaaacaata aggttaatat
    18601 ataatttgac tatattaaga cgaaaggaga catatacaat gaatacaata ttaaaagtaa
    18661 ataacataac taaaacttat aataaagggc aacagccgag tttagataag gtatccttta
    18721 atgtatcaga aggtgaattt ataggtataa tgggagcttc tggctctggg aaaacaactc
    18781 tacttaatgt attatctaca attgataaac caacaagtgg agatattttc ataaataata
    18841 taaatttgaa atcgcttaat gacacttgtg ctgctgattt tcgtaaggat aatttaggct
    18901 ttatctttca agagtatttt ttactagata gtcttacaat tttcgagaat atttcagtac
    18961 ctcttacact tcagaaactc aatccagata aaatagaaaa catggtaaag agtctagctg
    19021 aacgatttgg catagcttct caattaaata aatacccaaa tgaattatct ggtgggcaaa
    19081 gacagagagc ttcagcagtt agagcaatag taaaacatcc tagtattctc tttgcagatg
    19141 aaccaacagg agctttggat tctaattcag ctactgaact actccactca ctacaggaag
    19201 caaatgagga gttgaaaaca acaattttaa tggtaacaca tgatgcctat gtagcaagtt
    19261 ttgcaaatag aattttgatt tttaaagatg gtcatatcat taaagaatta aataaacaac
    19321 agaataatag acatacattt tatgagttga ttattcaaga aattgcaaaa ttggatacta
    19381 aaagatagga ggataattta atgaacattt tttatacggc aataaagaat cttaaaaaaa
    19441 acttttcatt ctattctcta tatctacttt cagtctcttt tgttattact gtatttttcg
    19501 catttacttc attttctgtt aataagataa tgctagaaaa aatttcaact gatggtagag
    19561 ttgaatctat gtgtaatgtt atttcgatat ttttgatggc atttgtcatc ttctatatgg
    19621 catactcaaa tcgattcttc ttgagacgtc gtacaaaaga attaggtata tatgctttac
    19681 ttggctatcg taagtttaca atattatcgc tacttacctt tgaaaatgta ttaatttgta
    19741 gtggagcttt tattattgga ttggtattag gagcattggc acataagggt attgtctttg
    19801 gaattacagc actacttaat ttatcaattg ataattcaca gataccattt tttaatataa
    19861 atgcaataaa taaaacaatt atctttatag cattagttgt tttagtctta gctgtatcaa
    19921 atggtaaatt tttttataag actccacttg taaaattgat aagatatgaa aaaagtgcag
    19981 aaaaaaaatt aaagttcaga aaaattccag cctttatggg gtttattatg attttaagcg
    20041 ggtattgttt atcactagat attcttcgtg gagctaaatc agtatgggta agtattggat
    20101 tttatcaaat gggcttgata actatgatta tcattgtaat tggtacagtt ctctttatca
    20161 attcctttct tccatatatt atgcaaaata gtaaaaaaaa taaacgtact ttctacacag
    20221 atatgaagat aatagctatt ccaaacttta tctatcgtat acgttcaaac tctaaaactc
    20281 taattatgct tacattactc tctgctacta ctttgacagt ttcaagtgtt atggcactgt
    20341 ccttatacta ccctattgca tctgtttcgc gtattgcacc ttcagagata gaatttagag
    20401 tagaaaaaaa taaacaaata gaagatgtaa aacaaattgt cagtcaatat acgtctaata
    20461 cagatgatat tatttttaca cagacaaata tttataaggt aaaatcttca tcaaaagacc
    20521 ttccatttga atatgaaatt ggtacatcta agaataattt atccaatagt agttcttcta
    20581 aagaagttat ttcaagagaa tcaggatttg aatgtatatc tttttctgat tattatactt
    20641 tattaaaggc acaaaatcga acaaagctac taaaacaact taccccttta agggataatg
    20701 aatgtatttt aatgaaatat caaccaaata atgatggata tagtgaaaat ggaacgattt
    20761 atccattact tgttaatggt aaagaaacac ctgtagctgt aagggaaaca tcattaaaca
    20821 atgctatttc ttttgccaat agcgttggaa cacttatcgt taatgatagt atatatgagc
    20881 aaatagcaca aaataaatta ccaatgacaa gaattttaag tattaatggt aaatcaatta
    20941 aaaataatga aaatttatat agtaatattt cagattattt agatggaagc ccttatttac
    21001 aaggatattc tcacaggata aatgatttat tatcaattaa tagttctaca tttctgttaa
    21061 tcggatttct tgtggtatta ttctttattg ctgttggaag tattttatac tttaacaata
    21121 tatcagcaat ttcagattca acatctgatt ataaaatttt acagaaaatg ggttatacta
    21181 atatacaaat taaaaaaatt attaaaaaac aagtattgcc attttttaat attccgtttt
    21241 tacttggtat agtggactgt atatttgcta ccttggttta taagactgct ttgatgcaga
    21301 atttacttgg aaattcactt tcacaataca tacctgtgat tattgcaatt ttcttaactt
    21361 ctgtagtata tttgatatat tacatactta ctattaaatc ctgttgtaaa attgtattaa
    21421 aaaaataata tttgccaaat cgtcaagctt aaaggtgtga cgatttggta tacttcattt
    21481 ataagtattt gatataattt aaaactataa attcttaagt ttgatttagt cataaaatta
    21541 atataattaa ctcataagga ggtgtataag attgacaaat tttaatttaa aaatactatt
    21601 tttaggaaac tacattctat cacttctctt catgggaata tctacaagca ctgaaactta
    21661 tatgaatatt caaagcatta ttcccttaat aatttcaaca ttattactca tagacatcac
    21721 atatataacc tcttgtaatg tccgagaaaa taacattatt tcattgtttt gtggattact
    21781 agcactagac agttggtatt tactcttatc atttgtaacc acaccaatag gcactattat
    21841 ttttaaaata ttaagcacag ttatagtgta tgtctctatt aaatttatat ttttattttt
    21901 gtttcaaggt agtggataca atttccgtaa gataacagac attttattgc ttatcacctg
    21961 cattggtgca attgtaggag catttatttc taacagaacg tatgcttgta tgtacggaat
    22021 acagtttgtt gttagtataa taagcttttt ctatattatt ttttatcatt tgaaaagggt
    22081 tgtttttgtc ataaaaagcg aaaaaaaacc tatatcgatt tcagtattta taacaattat
    22141 aggattcctt acttattatt tttttacact aagaatatca aatcatattg gtaattttgg
    22201 aatctatcta actgtactgg tattttttat gagtattcat ggtattgcac ttaaagaaaa
    22261 tgatagtttt cctatttcaa cagtttttaa cataaaacaa agggctatat ttctttttat
    22321 aagtatttta gtaattagct tggtcgtact atttgccaat gggaattttt ctataatatt
    22381 aatagctata aatgttttat ttatgctcat tttcctctgt aacattattt tggagtttaa
    22441 ccttagagat gaaaaaaata aagtagttaa aaatagcaag tatacttttg cattagaaaa
    22501 attacaacaa gaagaacaac taaaaacaga gttttctaat tttttacatg atgacatatt
    22561 acagaacctg ctttcaataa aaaacatgat gagtaagtca tatcgcccag atgttcaaga
    22621 tattatatat gaaacactta acaatctaaa tacatgtata aggaagcaaa tgcaagacta
    22681 tcatcctgtt attttaaaaa atttaacagt aaaagaaaat tatcagaatt taattgaaag
    22741 tgtttcttca tcctttccac aaaaaaatat ttgtgtttca tttaagtgtt ctgatacttt
    22801 attcttagtt gaaccatatg atatattgat atatcgactg attaaagaat tattgacaaa
    22861 tgtgtataaa cattcaaatg gtagccatgc atggatagct ttagcactag aaaaaaatat
    22921 aataaaatta aatattagtg ataatggtgt ggcaaataca tcttttaaaa cgcctttaga
    22981 tataactaaa attacttttg atacaataaa acacaaaggg attatatcaa ttaaagaaca
    23041 agtagaaaat ctaggaggaa cgataagtgt ttctgataat atacctcatg gagtttgtgt
    23101 acagattaca atccctatga aaggagatgg ttcttataaa tattttgtta gttgatgacc
    23161 atatgctttt tgctaaaagc ttgtcaattg cactagaaga atacaaagaa atcgaacagt
    23221 tttattccac acaggacatc cataatcttt cagaaatgat tcaagataaa aaaatagaca
    23281 ttgtcttaat ggatataaac cttagaggct taagtgaatg tgatggctta aaagtagctt
    23341 ataatttatt agagactaat tcaactatta aaataataat cttgactgga tacgacctcc
    23401 cagtttataa acatgaggct aaaaaaatag gtatctatgg attcctcaat aagaatatta
    23461 gccctgatag tttaattgat tcattattaa aagtctatca aggggtaact tgttttccat
    23521 ctgaaaatac tgaagtaatt attgaagatt taactactat ggagaaaaga attttacagc
    23581 ttatctgtag tggtaaaaaa agaaaaataa ttgctgatga attgtatata agtgaaagaa
    23641 cattatcaaa tcatttacaa cacatatatg acaagctaga tgtttcatca tcagttgagg
    23701 ctattacaaa agctattaaa atgggttata tttctccagt atattaaaaa aagtgagtgt
    23761 ctcaaaataa atcttctaac tcataaagca ctctcctttg tattaaatca aatatatttt
    23821 catcatttaa acaattaaga agaggcttct ctctattttg agacaactcc attttaatac
    23881 actatttata tttatatata aacatatttt aaacaactaa aaagcaacta atatctcaat
    23941 acatatcttc ataacacata tcaccataat tacaagcaga taaagaactc tcctcattta
    24001 aatacgactc aaattcatac ccaaacagaa ttgttggttc tatatatatt tccatatctg
    24061 tatatctcca tcttcttacc tttgcctcta taaccttgta aaaatcttct aaactatacc
    24121 cttcttttaa tcttttatct ataagtgcct tagttatact caaatcagct ttaaaatttt
    24181 ttcctgattc actatttaat tttcttataa taatttcatg tatgctttct ggatttttgt
    24241 aagtagaatc ttcagtcaaa tcttcatccg aattttcaaa ggtattttta tttaaattct
    24301 ttttatttaa attatttttt aataattctt ttttctttgt ctcattttct gtttcatacc
    24361 cagtatgaat tatcaaatca taatctttgt ctatactctc ttgatttata cggctgtata
    24421 tgttggtatt actgttctta aacaactcat ttctatgttt atatttcatt gttttttggt
    24481 tattgttaat agttgcatca atgtatttct ctatattttt gaaaatatca ccattctttg
    24541 ttacaatttc tgcattacta ctttcagaac aaacataact atatacagaa caacaatttc
    24601 cctttactcc cctacttatt aatctcaata tacctaaatc tataaattca tttacaagtc
    24661 tacttattgt agatatagat acatcaaggt cagatgcagc tttccttact gtcatcataa
    24721 attgtcctct tggcagagtt ccttttaatc tttgtatatt ttcttttctc attacatagt
    24781 gattaaacct tattctattt acatctcttt tttcattaaa atataatact gctgaattta
    24841 attgataaaa actattactc atattcccac tcctccaatt taaatagata atttgttgta
    24901 aagtttttag atttttaaat aacttattaa atgattattt ttattaaaaa gtattctttg
    24961 atattttcat tttaaagttt taagtataat aagttaatag tgtagattta acttttttat
    25021 ttaaacaata aacttaatat tatttttaat ataaaaattt tattaaattt agtatgtaat
    25081 tttttaatct tattttattc catctatact cttatgctaa gtaataactt tgataatatt
    25141 tccctaactt atgtttcaac tggtttatat tgcaatttat ttaattacag cttatttgca
    25201 taataattcg tccatatcat aagttgtgta tttatataaa tacacaatta aaaataaatc
    25261 actatggtat actaattatt acgaagacta tacttatatg caaagacaaa tataaggcat
    25321 cttatatctt ttattaatta taaatatctt ttattaatta taaatatctt ttatcaatta
    25381 taaaaaatta aacgctatct tatttatcat aaattacaaa aacataaaat tttattacta
    25441 tattgctata aattaattga tacacatttt acacaataag gagggctata tatgacaaat
    25501 gctaaaagta aaaaagaata tctaaatcgt atttgcaaag tgcaagacta tatagaagaa
    25561 catttacatg aacctctttc tcttgatgaa ctttctaata tagcaggttt ttcaaaattt
    25621 cattttcata gaatcttcaa agcaatagaa aaagaaactt tggcacaata tgtaaatagg
    25681 ctaaaactag aaaatgcaac tgcttttctt attcatagaa ctgacatgac tgttactgat
    25741 attgcacact actttggctt tactgattca gcagttttct caagagcatt taaaaattac
    25801 tataaaataa gccctataaa atatagaaac aattatagca agaattgcaa agaaccatat
    25861 aaaatttctc agtacaatag aagcatatca aaacctaaat gtaagaatac tagagaggtt
    25921 aaaggtgaaa ttgaaattct agaacttgat aatatgaata ctatttacac taggtatact
    25981 ggttcatatg gtaatctaac cagtactttc ccaaaacttt tagaacggtt attcacatat
    26041 gcaagtgagc agaatctact tggacttgaa aacacacaaa tattagccat ttatcatgat
    26101 aatccacaat ttacacagga gcatcaatta aggactagta tttgcatgac cattccagat
    26161 aacattgttg tcaaagagaa tgacaatctt ggcaatatga taataccttc tggaaaatat
    26221 cttgtgggac attttgatat ttcaaaatct gaatatagtg atgcgtggga ttttatgtat
    26281 aatgaatggc tatcaaatgg taattataag ctacgtgcct ccttcccttt tgaagtatat
    26341 cttaacaacc cacacaatca ccctcaaaat agacacttgg tcgatatata tttacctata
    26401 gaaccttttt aataacctca cctaatctat tcttgtatta aggattaata tatccctatt
    26461 ttacggtttg ataaaattga taaaatgagg tgaaccaatg aaagaaataa aaattaatgg
    26521 agcaaagata cacaatctta aaaatataga tgtttctatc cccaaagata agcttgttgt
    26581 tgcaacaggt gtcagtggct caggtaagtc tagccttatg tttgacattg tatttgagga
    26641 agggcgtaaa caatatctgc aatctttagg tatacttgca ggtattgata gtgaagataa
    26701 gtttgataat attcaaggtt tagctcccac tatagctgtt caacaaaata ttattcgcca
    26761 gagcaatcct cgttcaacag taggcacaag aaccaatatt ctcaacatgc ttacactatt
    26821 gtattcagtg gaaggtcaaa taatgtgtac tatgtgtgat actcctgtaa gctataatct
    26881 aatttgcaat aattgtggat atgaggaaga acgattaaga ccaagttatt tctcatacaa
    26941 ctcttcagat ggaatgtgta tgaagtgctc tggacatggt gcatattttg agattaatat
    27001 tgaaaaatta gtccttgata agcatgatac cctaaaacaa gtcttagata gagcaaagat
    27061 tacacctgga tatttgagag tattttctaa aaaattcaat gattatttag acatacaatt
    27121 cttacaacta cctgaagaag taagaaatga agtactctat ggtcattatg aaaatggtaa
    27181 aaaaagttcc tctttatcaa aggtatttca caatcgttac aaaaggggcg aagatttaaa
    27241 tggagtttat actatgactg cctgttcaga ttgcaatggt tttaaaattg gtgaagaagc
    27301 acgtagagta cttttaaacg gaaaacatat tggagaactt ggaaaaatga cgattttaga
    27361 aattgatgat ttccttaaac tcttaataaa tcaaggtaat ttaaacacat ttggtacaaa
    27421 tcttgtaaat gatattttat ccaaaactca acatttaata gaatcacgat taggtcatct
    27481 atccttatac cgagaaatgt caactttgag tggtggggaa atgcaaagac tatttttaaa
    27541 ttcacatctc gattcagaga tggactcatt aatttatgtg cttgatgaac caacagctgg
    27601 attgcatgaa tcagaaaaaa tagacatctt aaaatcaata aagaaactta aagatttagg
    27661 caatacggtc atagtagttg agcatgataa aaacacaatt aaaatggcag aacatattat
    27721 tgatattggt ccaaaagctg gtgttgaagg tggtcaatta gtatatcaag gagatttaga
    27781 aggtctactt caatccgaca aatcactcac tggacagtat ctatctggaa aatatccaat
    27841 gcctaataga acaagcaata aaaacattac acatactgat aaaatacctt gtataactat
    27901 tcaaaatgca aatacaaata atcttaaaaa tgtaactgta tcattacctt tgggtgctat
    27961 ggttggaata tctggaaaat caggtagtgg caagagttca cttatatcag acaccctact
    28021 tcctcttctt agaagccaat ttaataacca tactatcaat aatcaaataa attcttcaga
    28081 gagtgaaata ggtgaggaag acgattatgc agttgttgaa actattgcag atagactgat
    28141 tggtactaaa catatttctg gatattcaga gatatctcaa tctcctattg gcaaaaatat
    28201 gaactcaact cctgcttctt atattggtat atgggataag atacgtacac tatttgcagg
    28261 tcagccagaa tcgttagaac gaggatttac agctggtcat ttctcattta actccaaagg
    28321 ggcttgtcct aaatgtagtg gtagtggata tgaaaaggtc tggttgggaa ataatctaag
    28381 tatagaccat atatgcagtg aatgtcaggg aaaaagattt aatgatgaat ctttatctat
    28441 taaatataaa aataaaaata tacatgatat tttaaatatg agtgtttctg aatctgtaca
    28501 tttcttcaag gatattccaa atattgtttc acacttaaat gtattagaac aaataggtat
    28561 ggggtatata aaattaggcc aacctacacc aactttaagt ggtggtgaat cccaaagaat
    28621 caaacttgca aaagaaattg gtaaaaaacg aaatggtaat atcctttatg tattggatga
    28681 gccaactact ggtctaagtc tatacgatac ttctaagttg attcaattat tagatgaatt
    28741 ggttgaaaat ggaaattctg taattgttgt tgaacatgat atagatgtat taaatgtttg
    28801 tgactggatt gttgagcttg gtccagaagg tggtgacaaa ggaggttata taattgcaga
    28861 aggttctcca aaaactctaa aagaaaatcc aaaatctata acagggaggt atctttaatg
    28921 aataaaaaca attgcttaga tttaatattt gaaaaagtaa atccttcaag tgttggcatg
    28981 agttcagaaa aactctcaca acttgaacct ttaataatct cacaatatag taatataaat
    29041 ggaataatta ttgcaagaaa aggaaaagtt gtctttgaaa agtactataa tggatataat
    29101 gctgatgata cttttcatgt tgcatcagtt acaaaaagta ttatatcagc cttaataggt
    29161 attgcaatag ataaaggata tataaaagat gttgaccaaa aggttgttga ttttttccct
    29221 gaatatactt atagtccttc tgaaatatgt aaaaaagcag taactatacg tcacctactt
    29281 acaatgactg caccataccc ttttaaaaat atgactgagt ctttagaaag actttgcaaa
    29341 caaaaagatt ggataaaata taccttgaat caacttggtc aaaaaggcga aattggtact
    29401 ttcaaatatt cttcagctgg aacgcaccta ctttcagcta ttataacaaa aactactgga
    29461 aaatctgctc gtgaatttgc taatgaatac ttatttaaac ctattggtat gaaagttata
    29521 ccagattatg atatgaaaga ttttggtttt gaagatttct ttggaaaaaa tcttaagggt
    29581 tgggcaaaag atagacaggg ttattctata ggtggctggg gacttacatt gagtgctaga
    29641 gatatgttgc gatttggttt tctgtattta aatgatggta tttggaataa taaacgtatt
    29701 atatctaaat cgtggattga agaatcagtt aaaatgaata gtaatgaata cggttatctg
    29761 tggtggctat gtaaaaaaga tggtgttttg tggtattcag caatgggagc tggaggaaat
    29821 gctatttgtt gtattccaca atatgactta gtaatagcca taccatctaa aatagtgttt
    29881 aaacctcgtg atagatggga gtttattgaa aaatatatac ttacttctat aatagattaa
    29941 ttactctttc ataaattaat tactcttaga taaccaaacc ctccattata acaccagact
    30001 tttgtttggt gttattatga ctaatactat atatctaaaa ggagttgaat actaatggac
    30061 acttacacga catcagaggt agcagattta attggtgtac atcctaatac agtacgttta
    30121 tatgaagaac taaacttaat acctaaacct aagcgtaata ccaatggtta tcgtatcttt
    30181 aatgattttc atatagaaca atttagattt gctcgtacag ccttgaaaat tgaagtggta
    30241 caaaggggat taagaaaaga tgcccttgag attgtaagaa catcagcgaa tagggacttt
    30301 gataaggcta ttagtcttgt aaatcaatac ttgcaacaat tagaaataga aaaatctaat
    30361 gccgaggaag cacttactat tgttgcacag attttaaatg gaaatactcc agaaacaaat
    30421 aaactctttt taactagaaa agaaactgca gaacacttaa acgttacaat agatacttta
    30481 agaaattggg aaatgaatgg tttgttaaaa gtaaaaagaa aacaaaatgg ttatcgtgta
    30541 tatactgatg aagatataaa acgacttaaa attataagcg cacttcgttg tgcaaactat
    30601 tctctttcag ctattttacg aatgttaaat gctttatctt ctaatcctaa tactaatatt
    30661 aaaactgtcc ttgacactcc aaaagatgat gacgaaatta tctcagtgtg tgacaaactg
    30721 ataacttcat tgaaagaagc tgaacataat gcaaaaacca tgaaatctca tcttcaaatt
    30781 atgaaaaaac aattttccta gccctccact ctaactccag actttgattt gtgtgttatc
    30841 cttaagagca agaaaaattc aacacacaaa tcaaaggagg aataggatgg aaaacaaaaa
    30901 tactaattct acatggaaga aaaaagtcat tcttttttta gcaagtcaat atatttcctt
    30961 atttggctca tcacttgtac aaatggcaat gatatggtat gtcacacttc agacatcatc
    31021 aggtgtatgg gtaacagttt taactgtatg ctcctttata cctcaaatga taatttcttt
    31081 ttttgctggt gtatgggcag actactacaa tcgaaaggta ctcataattg tatcagattc
    31141 aacaattgct atatctacat tagttctaat tctatttatg atgtcatcaa atggcacaac
    31201 aagtgaatta atagccatat cagtaatatc tgttatacgt tcactaggaa gtggtgtaca
    31261 aacacctgct gtaaacgcaa tgattcctca acttgtacca gaagaacatt taatgcgttt
    31321 taatggtata aatagtagcc tacaatctat agtacaattt atagttccta ttgtagctgc
    31381 tgcaatactt ggatttggta ataaaataaa tcaagttctt ttaattgatg tatttactgc
    31441 aattattgct attgtattat tgatattcat caaaattcct aaacatgata taccaaaaca
    31501 aactgaaaaa gtctcatttt ttgcagatat ggtatcaggg ataaagtact gtttttcaga
    31561 taagttcatt agaaaagtgc tttttactta tggagcattc atgcttcttt gtgttccatc
    31621 aggatttctt ataggacttc ttattgaacg aacatttggc aagggttata tatacctgtc
    31681 tattattgaa atgattggat ttttaggtat ggttttaggt ggattgatta taggagtttt
    31741 tggtggtttt aaaaatagaa ataagacctt ttttctagca atattatcct atgctatttt
    31801 ttcaattatt ctaggtcttg taacacaagc ttggcaattt tttatattta tgttttttac
    31861 gaatttttca attccaatca ttcaagcatc agctatgact atgttgcaag agcatgtagc
    31921 accacaaatg ctaggtcgag ttcttagctt acctattatt atatatactg gttttattcc
    31981 tcttggtatg atgattttcg gaccaatggc agatgtaatt tctattaata atttaataat
    32041 catagcaggg attatacttt tattttttgc tttggctatc ccctattcaa aacaatttta
    32101 caagcaagga atatctaagt caaatgaatc cgagtaatat atttaaatag tttaactcga
    32161 tttatctggt taaaaatgag ggggcttaaa atggactttt taatttatga agaaaatttt
    32221 ttatatgaaa tcaaatatat tttatcattt caacattgaa aaataggttt atctttattt
    32281 tgagcccctc tctttatagc aaataatatt ttgatactat ctttattaac tttatataat
    32341 aataccttta cttgtaggtt ataatgatga aatttatcaa atcatataat aatacctcca
    32401 tttatatcat aaattaaatt tatagtacaa atggaggaaa ataaagtatt tatatagttt
    32461 aagtacctat taagttttcc actaagtagc tctaattgta ctgaacttct tactaaatta
    32521 tattgaataa ttataaaata aaactaacta ttacactaca taaattaaag ta
//
LOCUS       DAELTA010000027        32395 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.027, whole genome shotgun sequence.
ACCESSION   DAELTA010000027 DAELTA010000000
VERSION     DAELTA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.027"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            321..1142
                     /gene="yidA"
                     /locus_tag="KPG13_001083"
     CDS             321..1142
                     /gene="yidA"
                     /locus_tag="KPG13_001083"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG0984822.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1278..1427)
                     /locus_tag="KPG13_001084"
     CDS             complement(1278..1427)
                     /locus_tag="KPG13_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984823.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1434..1919)
                     /locus_tag="KPG13_001085"
     CDS             complement(1434..1919)
                     /locus_tag="KPG13_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984824.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1932..2684)
                     /locus_tag="KPG13_001086"
     CDS             complement(1932..2684)
                     /locus_tag="KPG13_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0984825.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2861..3490)
                     /locus_tag="KPG13_001087"
     CDS             complement(2861..3490)
                     /locus_tag="KPG13_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0984826.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3451..4014)
                     /locus_tag="KPG13_001088"
     CDS             complement(3451..4014)
                     /locus_tag="KPG13_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984827.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4024..4248)
                     /locus_tag="KPG13_001089"
     CDS             complement(4024..4248)
                     /locus_tag="KPG13_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984828.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4253..5344)
                     /locus_tag="KPG13_001090"
     CDS             complement(4253..5344)
                     /locus_tag="KPG13_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0984829.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5379..5837)
                     /locus_tag="KPG13_001091"
     CDS             complement(5379..5837)
                     /locus_tag="KPG13_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984830.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5830..6915)
                     /locus_tag="KPG13_001092"
     CDS             complement(5830..6915)
                     /locus_tag="KPG13_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0984831.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6942..7127)
                     /locus_tag="KPG13_001093"
     CDS             complement(6942..7127)
                     /locus_tag="KPG13_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984832.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7289..7621)
                     /locus_tag="KPG13_001094"
     CDS             complement(7289..7621)
                     /locus_tag="KPG13_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG0984833.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7877..8713)
                     /gene="nadC"
                     /locus_tag="KPG13_001095"
     CDS             complement(7877..8713)
                     /gene="nadC"
                     /locus_tag="KPG13_001095"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG0984834.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8694..9995)
                     /locus_tag="KPG13_001096"
     CDS             complement(8694..9995)
                     /locus_tag="KPG13_001096"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG0984835.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(10008..10922)
                     /gene="nadA"
                     /locus_tag="KPG13_001097"
     CDS             complement(10008..10922)
                     /gene="nadA"
                     /locus_tag="KPG13_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG0984836.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11340..12797
                     /locus_tag="KPG13_001098"
     CDS             11340..12797
                     /locus_tag="KPG13_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0984837.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13020..13448
                     /locus_tag="KPG13_001099"
     CDS             13020..13448
                     /locus_tag="KPG13_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984838.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13508..13816
                     /locus_tag="KPG13_001100"
     CDS             13508..13816
                     /locus_tag="KPG13_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG0984839.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13963..14985)
                     /locus_tag="KPG13_001101"
     CDS             complement(13963..14985)
                     /locus_tag="KPG13_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0984840.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15160..15666)
                     /locus_tag="KPG13_001102"
     CDS             complement(15160..15666)
                     /locus_tag="KPG13_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG0984841.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15726..16427)
                     /locus_tag="KPG13_001103"
     CDS             complement(15726..16427)
                     /locus_tag="KPG13_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984842.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16645..17715)
                     /gene="buk"
                     /locus_tag="KPG13_001104"
     CDS             complement(16645..17715)
                     /gene="buk"
                     /locus_tag="KPG13_001104"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0984843.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17768..18343)
                     /locus_tag="KPG13_001105"
     CDS             complement(17768..18343)
                     /locus_tag="KPG13_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG0984844.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18345..20132)
                     /gene="iorA"
                     /locus_tag="KPG13_001106"
     CDS             complement(18345..20132)
                     /gene="iorA"
                     /locus_tag="KPG13_001106"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG0984845.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20193..21452)
                     /locus_tag="KPG13_001107"
     CDS             complement(20193..21452)
                     /locus_tag="KPG13_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0984846.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21714..23714)
                     /locus_tag="KPG13_001108"
     CDS             complement(21714..23714)
                     /locus_tag="KPG13_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984847.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23722..25609)
                     /locus_tag="KPG13_001109"
                     /pseudo
     CDS             complement(23722..25609)
                     /locus_tag="KPG13_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(25636..27477)
                     /locus_tag="KPG13_001110"
     CDS             complement(25636..27477)
                     /locus_tag="KPG13_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0984848.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(27608..28027)
                     /locus_tag="KPG13_001111"
     CDS             complement(27608..28027)
                     /locus_tag="KPG13_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG0984849.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            28340..28522
                     /locus_tag="KPG13_001112"
     CDS             28340..28522
                     /locus_tag="KPG13_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984850.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            28851..29873
                     /locus_tag="KPG13_001113"
     CDS             28851..29873
                     /locus_tag="KPG13_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0984851.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(29966..30568)
                     /locus_tag="KPG13_001114"
     CDS             complement(29966..30568)
                     /locus_tag="KPG13_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0984852.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(30585..31733)
                     /locus_tag="KPG13_001115"
     CDS             complement(30585..31733)
                     /locus_tag="KPG13_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG0984853.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            complement(31726..32103)
                     /locus_tag="KPG13_001116"
     CDS             complement(31726..32103)
                     /locus_tag="KPG13_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0984854.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT        10643 a         5555 c         3466 g        12731 t
ORIGIN      
        1 actaatataa caaattacca tcagtctatt tattcaaact tctaactata taatatacat
       61 ttatttatat taacaatgca aacgatacca ttatttgtaa ttaagaattt tggaattatt
      121 gattacaaag tttttacatt ctcatctcac ataagtaata tttttatcaa aaaattaata
      181 ttattaaact ttctaaatta cttatctctt gatataatag tatacaaaac aaaccatatt
      241 tcccactgaa actttgttca acactagctg tgtcaacgtt ttcacaggaa atttgagtat
      301 ataatttatt ggaggttgat atgtacaaat taatcgcact agacattgat ggaacaatat
      361 taaatacaca aaaaaggatt actcctgaag tctttgagtc cattcaggag gcaaaaaggg
      421 ctggggcaaa agtagttata acaacaggca gacctcttcc tggtgtaaaa gaacttctaa
      481 atcaactaaa tttaacagat gaaggtgatt atgttatatg ctttaatgga gcaattattc
      541 aagaagttaa aagtgagaaa atcatacatg atgtagaaat gagtttagat gattttgatt
      601 ttatatataa taacgtttgt aaaaaatata agactaaaat acacataaat acaatgacaa
      661 atttaattac accaaatgaa acgcctggca aatatacact tcatgaagcg aaattaaata
      721 atatagaagt caaatatata caaaaagata aaattgatga atctattaaa atctgtaaaa
      781 ttatgatagt tgatgaacca gaaagattgg aagaaatcat acagcaacta cctaaaaatt
      841 tattcaataa atatactata gtaaggtctg ctccttttta tctagaattt ttaggtaaaa
      901 ctactaataa aggaactgct cttaaaacat tatgtacgaa tctaaatata cctatagaaa
      961 atgctattgc tgttggcgat gaagaaaatg accaacatat gataaagtat gcaggtcttg
     1021 gtgtggctat ggggaatgca cgtaatagta taaaggaaat tgcagattat gttactgata
     1081 ctaataatga aaatggagtt gcaaaggtta taaataaata tatacttaat aaggctatct
     1141 aatcatctaa aaaaactaaa aatatacctt tagatttaaa tacttttcac atccctactc
     1201 tatactaatt tatatagtat ggatattacc tatcattttt atctaaaggt catttttagt
     1261 ttttcatata tgtatatcta tgatagtacc ttttgtttga gtttaaatat tttatatgat
     1321 tctactattg cctcattaat atctaaacaa tatatatctt catcatcaga taatcccgct
     1381 tcgtttgtcc aagcatcaaa atcactcttc gtccagaaga aattcattat atttcagcaa
     1441 ctagcagccc aatttagatg cttatcaaga tttatatgaa gcactcttaa gtttggatta
     1501 ttcacatact caatcttttc attttttata attacttcta tttccttacc tgtagcacta
     1561 caaatagagt ttatttttat atcttggttg aatgtgcaag aggttcctat agcatctata
     1621 gcacacattg cactgaaact tctattatct ctcaaagtta ctttatgatt tgttggcaat
     1681 gctgatacag gatatataaa atttatacta ttcccatcaa caaccattat atttttataa
     1741 ataaaatatt gtagtgtcct ttctatatat tctttttcta tattcaactt tttacttaca
     1801 tattcagtca cttcttgaag tgtaacacta gaacctttat ctattatcat atccataata
     1861 gagattctta ttttctgttg tgtattatcc aaactattgt atttttctat ttgactcatg
     1921 tctactcctc tttaaacttc ataacacttt gataaagtat cagttaattt gtttttgtac
     1981 tcttcatatt tttggcttcc tctaattcta ggtcttggaa tatctatatt tatatcatca
     2041 ataattcttc ctttatctct cgacattact ataactctat cactcatata tacagcttca
     2101 tcaacatcat gagttaccat taatgcagtt attttcttct ttatccatat tcgttctagc
     2161 tcttcctgta aattttgtct catttgaaaa tctacagctc ctaatggttc atccattagt
     2221 aatacctctg gattacatgc taaagctctt atcatggcca ctctttgttt cattcctcca
     2281 gataattgat gcggaaacag tttttctttt cctttcaagt ctgacataac caaaagttct
     2341 tctagcattt cttctctttt ttcttttggt attttctgct gtttcatagg aaatagaaca
     2401 ttacctctta tagttttcca aggaaataaa gcataatttt gaaatataaa agctctatct
     2461 atgcccggtt ttttaactct tttatcattt ataaaaacat ctccacgttc tgatttttga
     2521 aacccaccta ttatagtaag taaagttgtt tttccacaac ctgatggtcc taaaagacag
     2581 acaaattgtc catcatctat atccatactt atatcttcta aaacctttgt atgtactcta
     2641 ttatttacaa atgtcttatt tatatctctt acacttaact tcattaatct gcaaacctcc
     2701 atctacaaag gattttattt ataaacttta ataccctatc tattataaat cctattaaag
     2761 ctgcaaatat cataagccca attaatacat ctgtatgcat taatgtttga gcttgtacca
     2821 ttgagtaacc gagaccggca ctcgtagcta taaactcggc tcagataaca gacatccatc
     2881 ctgtacttat agcaatcctt gaaccagtta gtatatctgg aagagatgcg ggaacaatta
     2941 catttgtaaa tatcacaatt ggcgatgctc ccatacttct agctgcatta taataatcct
     3001 ttgatattgc tcttactcct tgaatagtat ttaaaattat agggaaaacc cctgaaaaag
     3061 ctattaaaaa tattgttgga ccatctccta taccaaacca tactatagta agaggaaccc
     3121 atgccattat tggaacttgc cttacagaat ctactactgg agataaaacc ctttcaaaag
     3181 ttttagaaaa tcccataata aatcctattg gaagcccaaa caataatgca tacataaaac
     3241 ctttaagtac tctctgcaat gttataaaaa gattagtgac tgtctctgca tctgtcacac
     3301 agaaaaagaa accttttatt acatctactg gcattggaag taacaaagaa ctccctatat
     3361 cttttgctgc aaaatgccag actaaaaata taataactaa tagtattatt ttatataaaa
     3421 tatccatact accatcagtt tttttcttag ttaatctatc gcttttatct ttagaaattt
     3481 taatttccaa ttgcttattt cctccatatc tatatttctt atattcctgt actcttttaa
     3541 tactttctta aatgtagctt tattctttaa agcatcaaca cttttattgt aaagtgttat
     3601 aaaatcacta tatgctttac tttttttaaa ctctttattt acaactaata cataggtatc
     3661 ataatctcct aatttagttg tagattttct tttaccctct aacccaattg actttatgca
     3721 atctattaca acaccatcta ctaaattact ttctaatcca tatgctagtg cagtccctaa
     3781 taagggagct acttccacat tcttgtagta ttttttaacc aattctgact ggtaatctct
     3841 attttgaatg actcctatct tttcaggatt attatcactt aatagaaata tatcagaatt
     3901 ttgaactaca gtaccaacaa tttcaaatcc actatttaca tttacatatt gttttgcagc
     3961 atctttacat atgattgcta tatctatttc ctctgaactc agagcccact gcattgtact
     4021 tgctcagcaa tcattaattg taaaaatatc cattatatca cttactttta tattttcaaa
     4081 gtagtctcta tttaccatat aatttattac aaaaccagag gtatcatcag atacccctat
     4141 tcttaatttt ccttttattt cttctttatc ttcaaaatta aaaaatatta taaatatact
     4201 tcccaaaata atgataaaaa gtattattat atttttcata ttctccattt atttataata
     4261 cctttgcttt ccagtcttca taactcatac cttcaggaaa ctctgggtta actttttctt
     4321 ttataaattt gtcaaaatca tcagctccac aagagtccaa taagcttgta tcaatcgtat
     4381 cttcatattt aggtatatca tctaaagcct ttaaatcttt atacatttgt aaattatttt
     4441 tatattcttc acctgtaact ttccaagtaa gagttctacc ttctccaaca gtcttcttat
     4501 agatagttct aagtgcaact tcttcttcta cattataata ttttgcaaat attttagctg
     4561 cttcagctgg atttgtatat atatattcta ttgcttttgt atgtgctaga accaattttt
     4621 ttgctaaatc tggatgttct tttataaagt ttttgtttaa tgagaatgaa caacagttat
     4681 attctttgcc attagctttt tctttatatt gagtagatgc tattattttt cctgcaccgt
     4741 cattttcagc tacagaaccc caagggtcac aagatgtaaa agcttgaatt ttcccagttt
     4801 taagagctaa atatttatct ttgtctgaat ccatatttac aacttcatat tttgatgggt
     4861 cagttggtag acctgtttct ggaccataat cacttgtcca taagaaatca ctcatatctg
     4921 ttgcaatttt tttacctatt aaatctttag gttcttttat atcttttgaa actattaaat
     4981 attcagaacc accagtatgg ttgttagctg ctattgttat tggcgaaccc ttaggtattg
     5041 ctccaactaa ccccttagtt cctatgtaac ctgcatccat ttttcctgct gccatagctt
     5101 caggaacttt accatttcca acagttttta cattaagacc taaatcttta tatattcctg
     5161 ccgattctgc tactggtccc gcagtcatat ggtcacaatt ataataacct actgttattg
     5221 tataatcatc ttttccagtt tttgcttctt ctttttcttt tggtttacta ctacatcctg
     5281 ttaatataga tgtacccatt acaccaaata agattaatgc ttgaatcttc ttagataatt
     5341 tcatataaat ttcccacctt tatgtattat ttttattttt agcaacattt gtccatatct
     5401 tcgccaaata aatcaacaaa ttttttaccc tctactctgt aaatagcata tcctacatta
     5461 ccatttttag accctgatac aattacttct attggtgctt tatcatcaat atttttaaag
     5521 gttatcgttt gattctctat aggtgctggt attggctttg ttatatagtg gctattttta
     5581 tcagaaacta cgactaccat ttcatttgaa ttattgcttt ttttatatat aactactaaa
     5641 tctttttttc catcattatt taagtcttct tcagcacaag ttattatgtc ttctttataa
     5701 ttttttctaa aatattctaa tgtttcttta ttcacatttg gaatagaagc atcttctttg
     5761 atgttatcat caactatttt tgataaaatg attaacacaa ctactataca ggatattata
     5821 actatctttt taagcatgat ttcctatgac ctctttcatc tttggtatta ttgatttaaa
     5881 tgcaaataaa ataattaaaa tagtacatac atactcaaaa ctatcaggta tatttaacat
     5941 tagtttattt gccaactgta ttgaagaatt tgttgcatct aaatttactg agttattatt
     6001 cactaaaagt aaatttgtta tatatccaat tattattgaa gacagtgtca aagttacaga
     6061 atatataata acagttcttt ttccaataag tttaaacata cttataagtt ctggtagatt
     6121 tgtagctgct ccagctatta gaaatgttat cgctatacca ggagatgctc cacttgcaac
     6181 aagggctgct ataaacggta tatgtcctac agcacataca tacataacac atgcaagtat
     6241 tgctatactt ccaagagata gcaaacctgg atttcctaaa tatttttgaa ttatactatt
     6301 tggaaaaata gttgttataa atcctgcaaa aaccataccc attactacat atttactaat
     6361 agtaagggct aattcaccaa aagaccattt aagaccatca atcatatttc ccataaaact
     6421 tcttttttct tcttctaatt ctattacttc ttcctctgaa tcattatgaa gtgataattc
     6481 ttcttttcca aaacaatttc ctaaaaatcc tactaaaaat ggtactaaaa aaccagctat
     6541 tagataaatg attgtaactt taggacctaa aagacctaga caaagaacta cagctattgg
     6601 atttattatt ggagttgatg tcataaaagc aagtactggt cctaaatatg ctcctgaata
     6661 atacatactt attccaagtg gtattactcc acaactacat attggtaaaa acattcctga
     6721 gatagtactt ttaattaaag aagaaaattt cttatttcca agttgttttt gaaaccttct
     6781 tggagttaaa acattgtgaa gtacacctgc caatataaaa cttatcataa gccatcctga
     6841 tgctccattc agtgtaacta tagaagctac tatagtatct ttaactccat ttaaaacaat
     6901 ttctaatata ttcatttttg atactcctaa tttttaaact tttaattatt gtttatagct
     6961 tctactattg ccctttcaat tgtatcttta tttaatctat catatttctt tttctgattt
     7021 attattaaag ttccttttgt tataatccca tattttttta tatacgaaaa atctttccct
     7081 gcttgatata attttatttc caatttatca gcatattttt ctgacatttt ttttataaac
     7141 actccatgtt catcacaaga tggtcaagtg tttataaatt caactaaaat tttagacatt
     7201 tgttaatccc ctactttaaa caatttatct tttacttaaa ataatccaca gaaaataaat
     7261 ttttctatgg attgtatcct atactaattt ataacgttat aacttggtcc acacctgcac
     7321 tgcctaaaca agcatatagg tttgggaaac atccaattgt agctccatca accaaattat
     7381 tctctatacc tctttctata gcacattggt cacaagccat tattatcata tcagtttttt
     7441 catgtaattt acttagtctc tcgcctacag gattaccttt agataaaaat aaggtattat
     7501 ccataaagaa aaacatccct agaacgtcta ctccatgagt tccttgttca agttgtggta
     7561 ttatcatttt gtctagaatt ttatgtgtat tagtactact aaaaatataa gctactttca
     7621 tttttattcc ccctatactt tttatatttt acaaaatttt acagatacaa ttatattata
     7681 agtttattct tatttatctg taaaatagat attaactata ttattaaaag tgtatatagt
     7741 atttatctat ttttattaaa ttattgattg atttttttct atttttatta attttaatga
     7801 ataaaaaata gactccttaa atttataaaa caaatttaag gagtctattt taattaagtt
     7861 tatttcaata taaaatttat atatttctca aattcttcat acttatatcg agagctttaa
     7921 cagaatgtgt aagcgctcct acagaaacaa catccacacc aatttttcct atatcttcta
     7981 tagtatctaa atttacattt ccggaaaatt ctacaatggc tcttccattt ataatatcta
     8041 ctgcttgttt tgccatttcc aaactcatat tatccaacat tattatatct gcattagctt
     8101 ctattgcttc ttttaccatg tctaaagttt ccacttccac ttctatcttt ctaacaaaag
     8161 aagtattttt tctagctaat tcaactgcct tcttaacact accagcagcc ccaatatggt
     8221 tgtctttaag caatattcca tcagataaat taaatctatg attgcatcct ccacctactt
     8281 taacagaata cttatctaat attctaagat taggtatcgt ttttctacta tctaataatt
     8341 ttgtatttgt accttcaagc ttttcaacat acctacttgt aatagtagct ataccactca
     8401 ttctctgtaa ataattcaat gctacacgct ctcctacaag taagtttcta gtactacctg
     8461 ttaatacagc tatcttttca cgattcttta ctctatcacc atcattttta tataatttta
     8521 catctacatc accaagtatc tcaaaaactc tcttaaatac acctaagcca gctattatac
     8581 catcttgttt acaaattaaa tctacagtag atttagaatt ttcatccaca atagaattag
     8641 ttgttatatc ttcacttgga acatcttcta tcaaagcatc ctgtatcatt ctatcaataa
     8701 tcaaataatt catcgccaaa atcctcactt gttctttcaa tttcttttat gactaatatt
     8761 ttattttctt tttttacctg ttctatatct atatccatat ctggtacatc tataaactta
     8821 acttttacat tatctataac tgagtttata tttttagctg ctctttttga aaatacaagc
     8881 ccttcaagta atgaattact tgccaatcta ttggctccat gaacacctgt acaactaatt
     8941 tcaccaactg catataaatt ttccatagat gtttttgaat ccaaatctac atgtatcccg
     9001 cccataaaat aatgttgtgc aggagatacc tttatacatt ctttcgttat gtctgttcct
     9061 ctagctaaac attcattata aataaaagaa aatctattaa ttaaatattc actatctaaa
     9121 tgtgttgcat ctaaatacac ataaggtaat ttatcttttt ccatttgctc aaaaatagct
     9181 tttgaaacta catctctagg aagaagttca tccacaaatc tttctttatt tttatttaaa
     9241 agtttagctc cctctcctct taatgattca gatataagca ttctcctacc ttcattattt
     9301 tcttcataaa aggctgtagg atgtatttga atatattcta aattttctat ttttatatta
     9361 tttcttaaag ctattgcaat tccatcacct gtaagatgtc tttgattagt agaattttta
     9421 aaaagcccac caattccacc tgtagctaaa actactattt ttgaaaaaac atgaatctct
     9481 tttttagatt taaaaattct agctcctata catttattgc catcttttaa tatatctaaa
     9541 agataggcat cttctataag agttatattt tctctagttt tagcttcttt taataaagtt
     9601 ttaaaaacaa cttctccagt attatcctta ctatgaacta ttcgatttac actatgtgct
     9661 ccttctcttg tgtaatctat ctctccattt tctttgtcta aaggcatacc atattctact
     9721 atctgcccta cattttctat agattcatct gcaagtactt gaactgcttc aactctattt
     9781 ttatactgcc cagccttcat tgtatcttct acaaaggatt ctatatcatc aatatttcta
     9841 gcagttgaaa ttccaccttg tgcaagatat gtattgttgt tttcaattgt tgatttcgat
     9901 actactagca catttaaact tttatctaaa tttaatgcac agtataatcc tgaaactcca
     9961 cttccaacta ttaaaacatc ttgttctaaa ttcataagtc tatccctcta tccttctgct
    10021 aatttgtgca tgttaagcag tgaatttaaa gccttttcca tgatttcatc ttctacaata
    10081 acttcatttt ccatattttt aagtgtacta tataaatttt ctaatgttgt ctttttcata
    10141 tctgtacata gtatttttcc accaggaata taaaagtttt tattaggatt atttttcttt
    10201 aattcatgta atataccttc ttcagttgct attataaagt ctttgttttt actattagta
    10261 gcatatttta ttattcctga tgtactacct acataatctc ctaaatctct tatttctttt
    10321 ttacattctg gatgtactaa tacttctgca tttttatact tatcttttaa ctcaattatt
    10381 tcttcagctc ttatattgtt atggtattta caatatccgt cccaaaatat aaagttttta
    10441 tctggaaatt gttctgcaat atatcctcct aaatttttat ctggaaggaa tataatttct
    10501 ttattatcta tgttttctag tattttaatt gcactagaag atgtaacaga aacatcacaa
    10561 tgtgccttaa ctttagctgt agagtttata taacatacaa ccaaagcatt tggatactgt
    10621 tgttttaatt ttatcacgcc ttcttcatct accatatctg ccatagcaca cccagcgtta
    10681 cttactggca tcaatacagt tttttcaggt gataatatct tagcactttc tcccataaac
    10741 cttactccac aaaatacaac cacttcttct ttacaatctc tagcaatctc acttaaataa
    10801 tatgagtcac caactgcatc tgctaattct tgtatttctg gtggttgata aaaatgggca
    10861 agtattattg catttttttc tttctttagc tctttgattt gatatgttaa atctttatcc
    10921 ataattgcac cgctttctat atgtactagt gtatatacac ctgtaaactc attatatcac
    10981 ttgactattc tttcttcaag aattttaaac ctcttaatat tgttaagata tagacattta
    11041 tattctcaat tgattttcct tttttctcca tctatttaaa ctaattttta tattttttaa
    11101 tttttttgta aaatattatc agaattttgt ctatattctt tattgctata acattgaaaa
    11161 tattaccaaa tacctgtata ctaagtacat ggttatgtat atgtatttat ttctgaaatt
    11221 atattagttg taatacttta tagaatttgt aggttgtcca gaattaaatt caatataaat
    11281 ctacacattt agataatatg tatacaaaga ccaaaaaatt ttataaatgg ggtgatatta
    11341 tgacaacatt cttaatcggc ttggctatat tactaatagg aggtgcttta tacggtgctt
    11401 attgtgaaaa agtatttggc ccagatgaca gaaaaacacc tgcacttgct caaagtgatg
    11461 gagtagacta tgtcccaatg aaaaaatgga aaaatagtct aattgagttg cttaatatag
    11521 ctggtacagg accaattcta ggacctatcc aaggtatatt gtttggtcca atagcattca
    11581 tactaatacc tattggctgt gtatttggag gagctttgca tgactatatg agtggaatga
    11641 ttagtataag agaaaaagga gcacaaatgc cttctcttat cagtagattt ttaggtaata
    11701 aagtatttca agtctataat atattccttt gtcttttaat gcttttagtt ggtgcagttt
    11761 ttatttatac acctggggat ttagttgtta ctcaaatttt aaatatgaaa tcgactataa
    11821 ataatccagt tgtctggata gtttatggac ttatctttct atattacttg tgtgcaacac
    11881 tatttccaat tgataaaatc ataggaaagg tatatccaat atttggagct attctacttt
    11941 tatctgctgc tggtgttgga attggaatct ttacacaagg ttatgattta gctaatttaa
    12001 gtcttgcaaa ttggaaaggt attcatcctg atggtattcc tcttattcct acattctttg
    12061 ttacagttgc ttgtggtatt gtttctggat tccactcaac tcaagctact cttatagcaa
    12121 gatcagtttc aaatgaaaaa gaaggaaaaa ctacttttta taatatgatg attttagaag
    12181 gtttaattgc aatgatttgg gctgcagctg ctatgggtat ttacaataaa ggaataccta
    12241 aggaattagt aggttctcct gatgtaatag gtttggttgc aagagattta cttggttcta
    12301 ttggtggtat catagctata ataggtgtaa ttgttttacc aataacttct ggagatacag
    12361 ctttaagatc tttaagatta atgcttgcag attactttca ttatgaccaa aaggaaaaaa
    12421 aacatcgtgt tatattgtca atctgtatat ttattcctgt catagccatc cttatatttg
    12481 caaagcttag tgcttctgga tttaatatat tgtggagata cttctcttgg agtaatcaaa
    12541 ctattgccat atttgcattt gcaatgatta cagtttacct tataataaaa gaaaagaatt
    12601 atattataag cttaattcct ggtatgtttt attcctttgt tatattcagt tatatattta
    12661 atgctcaaat tggatttaac ttaaacataa atatatcata tgttttagct gccatattta
    12721 ctgtattata tgctatttta actgtacgct ctggtagaaa gttaaaaagc aaagcagata
    12781 ctaagttggc tgactaaatc taaataaaat aatacttaga ataaatttta ttaaaaaatg
    12841 ataaaaaagt aggcaatctc aagttagatt tgcctctttt ttatttaata cagtaataaa
    12901 tacttaagta tttactcctt attggaatat ttatcctaaa tctctaatat aaataaataa
    12961 aagttttaaa ataatctatt cgtttatata cttaaaataa attttttgga ggaatactta
    13021 tgaataataa attacatgaa cttgaaaaaa atctaccaga aacatcttgt tctttttgca
    13081 cacatctttc atttaagggg cctagcctag actatagata tgatattaga tgtgttattt
    13141 cagatacaaa gcccgatttt agaggatgtt gtgaatattt tgaaccagaa tatacagaat
    13201 taaatacagg agatttagac aatctataca taaactttct tgagacatgc ctcaaggtaa
    13261 attataatga ttacatcaat tctatgtatt ggaagttatt taaagaaaga accctactag
    13321 aaaacaattt taaatgttct atatgtggat caactgaaaa tgttgatgtg tatcatgtta
    13381 ataaaaattt aggaagggaa acttccaaag atgtagttgt taaatgtgat aaatgtaatt
    13441 gtaaataagt gtataaaatt tagataactt cacataataa gtttaaataa aacttaggag
    13501 gttatttatg aaaaataata atttaaattg tgctgctact aattgtgctt ataatactag
    13561 tggatattgt tatgctggta gtataaaagt agatggaatg caagcaacta ctacagaaaa
    13621 tacttattgt gcttcttttg aagacaaata tacatctggt ataactagtc gttctaatga
    13681 aactaaccaa gttgatacag ataacattca ttgtgaagct gtaaaatgta aatacaataa
    13741 aaatgaactt tgcaaagctg aaaaagttca tataaatgaa agaaatgcaa gttgtgaaac
    13801 ttttgaaatg aaataaattt ctttttattt agttttatag tatttataca aagcaaaatt
    13861 gaacctatca aatagatgtc tttattaatg atgtctattt ataggttctt ttttaaaaca
    13921 atttataatt tatatataaa gataaattat tattttaata tattacctag aagtaaattt
    13981 aacacccaga atatccataa ggaacataaa tattggaact ccaattagta atccccatac
    14041 acctaaataa tgttctccta caagtaaaat aataaaaaca aaacaaactg gaagtttagt
    14101 tctattcgac atcaacttag ggtttaatat gtatgtttct actgcatgca ctatacaaat
    14161 cataagtaaa actccaaaaa cctttgttat tcctcctgta ttaaatgcta ttacagttaa
    14221 tggaatcaaa gacactataa ctcctgctac tggaataaga cccaacacaa aaatcataaa
    14281 tcctaaagcc catatttgag ggaatcccat gaaacctaag aaaatcatag aaacaacaga
    14341 atttataaag gcaatcataa cttgtacttt cattacttca ccaaaatttt taacaaagct
    14401 ctttccaaaa aatacaagcg aattataaat aaaagaaatc ttactatcag aaagtttatc
    14461 tccaaaattt ttaatttcat ttttttcaag taaaaggagt aaactcagta aaaatgccaa
    14521 taatatattt actccaaaac ttccaacttt tgttgctgtt gatgctataa gtagccctgc
    14581 ttgtgaaata tatttattaa aatccagctt tgagacaata ggatacaatt ttggtcctaa
    14641 agattgagca aattgattta catcaaaatc actaaaaacc ctactcaact ctgtaagttg
    14701 aactataatt attggtacaa ctgcatagct aatcaatact aacaatatag caaatagtgc
    14761 atataacact ataattatta atgaatctgg aatatttaaa gaagaaaact tcttaattcg
    14821 ttttcttgtg gctacaagca agttatagaa aataaaagtc ataataaatg ttaaaagcac
    14881 catatttaac atataccaaa atgtaattag aataattact acgacagata tagaaacaat
    14941 tttatatttt gaactgtcac gtgccaatcc tttgttatct tccatagttt aaatcctccc
    15001 caaattttta tgactttata atgactgccc tttttatttt aatctcaaaa aacaagtaga
    15061 aaaatactaa aaataactta ttaattttca taaaacaata ttaaaattta gagaagatac
    15121 attttaaata atcttactta tactgaaatc tgctctacat catatattta atttattatt
    15181 atctatctca tatagcaatt cgtatatctc tggatattca aaaaacaaac cctcttttag
    15241 taattgtctt atttcatctg tgcttaccca ctttatatca cttacttctt cctcctgtaa
    15301 aatagctttg gatatatcat attctttttt aactaagtat acatcccaaa gagtatcttc
    15361 atgaatcata cttctaaaaa ctttcatctc atctttagtt atatctatcc ctatttcttc
    15421 ctttgcttcg cgaattgctc cttctaaact gtcttcccca gaaacaatac atccagttgt
    15481 cattccccac atatttggca atgtcttttt acttttagac cttttctgaa ttaaaatttg
    15541 agagttacta tttaaaatcc acacttcaac tgccaaatgg taataacctt cttcaataga
    15601 attacctctt tttataacat ttcctgtttt aattccatca gcattatata aatcccataa
    15661 ttccatttca aaatctcctt gactttacta taaattagat aaactacaaa tatattttag
    15721 cataattaat tctgctcatt ttttattaca atacttgatt tacatttatg ttcattgctt
    15781 ttcttttcct tccattctct tatatcatct ataataaatc tatgtttcaa caattttgtg
    15841 tcaaatttaa atattctaaa cactatttgt aatacatatc ctaaacctaa agcacttaca
    15901 agcgtaccaa tacctacaga acctcctaat aaccacccaa caactaatgc tagtatttca
    15961 agaattgttc taacaagacg tacagattta ttagtttttt tctgaagagc aatcataagc
    16021 ccatctctcg gtccacttcc taaacaaaca cctaagtata gaaaacttgc tatagctgcc
    16081 aaaataattc ctataacaac cataaacaca cctatataag tgttgcttgc atgaggtatc
    16141 actccagaaa atattactaa atcaataaaa attcctataa atgtcatgtt gagtaaagta
    16201 ccccatccta tattttcacc aaaaactgca tccaatataa caattacaac tcctaccatt
    16261 atagaagcct gacctatagt tatgcctagc tttatagata aaccttggtg aagtacatcc
    16321 caaggcataa gacctacatg tgcattaatc ataagcacag aagaaattgc aaatataaaa
    16381 aaacctgaaa ataatcttat catacttttt aaacttattt ttcccatcaa aaatcactcc
    16441 tcataataat aataacaata aataccaata aaatatatat ataaatacga aattttacag
    16501 aattatttat taggttaaga taaaatttta attataaaaa gtggcaatcc aaattttata
    16561 cattttcacc tatactctaa gctttcgtgt ataaaaaacg gatataccac ttttttagta
    16621 tataaacatt tttagttttt cttattaatt ttcactataa attctagctt cttcttctcc
    16681 atttagtact cttaaagttc caaaagcaag tgattccaac tcattttctc ctgccataat
    16741 ttctactggt gctataaact caactttctt tttaaccatt gaagtaaaca tttcagaata
    16801 tgcaattcca cctgttataa ttatagcatc tacctttcca tctactacag ttgctaactc
    16861 tccaatacct tttgctaact gataagccat tgcttcataa ataatttttg cttcttcatt
    16921 tccttctgct atcatttttt caacttctct agcatctaca gtatttaggt atgaaactat
    16981 accaccttta cctcttatct ttttagtcat ttctctttct gaatattgac ctgaataaca
    17041 ggcagccact aattttgtag caggtactct tcctgacctt tctggagaaa atggtccttc
    17101 atcatcagat agcatatcaa ccattcttcc tttttcatga agataaatac ttactcctcc
    17161 accaatgtga gctactatta agtttaagct cttataatct tttcctttat ccttagcata
    17221 ttttaaagcc attgctctag tatttaatgc atgccctgca cttgatctat ccattccaca
    17281 aagtccagat attctcgcta catctataag ctcatctact gcaacagaat cataaatata
    17341 tgagttgatt ccaagaggtt ctgatattgc atgtgccaca acagcaccta aattggaagc
    17401 gtgttcaagt actggtctat gtcttagtac atcaatcatt tcttcattta ctaaatatgc
    17461 tcctgatttt actggtggta aaactccacc tcttcctact attgcactaa gagattttaa
    17521 gtctatacta ttttctttaa gaatattcaa aactgcttcc ttacgcattt caaactggtc
    17581 ttgtatagta ttatattttg caatctcttc agctggatgg tctatcgtct tcacaagaat
    17641 ttgttcttct ccatcatata ctgctatttt tgtagaagta gaacctggat ttatggctaa
    17701 tattctgtaa gtcataattt tcttcctttc accttaattt ctatagtcta tttatatcta
    17761 ttataaacta tttgctaacc atttccatac cttcatggaa agcttttatg tttaattcaa
    17821 caaattttgg tttaacattt tcacttataa ttttttccca atcaatagac tctaattgca
    17881 tagatttaac tagacatcct aataaaataa cattcattat ttttgaattt cctagttctt
    17941 cagctttatc agctgcattt ataacttttg catttaatct acttaactct gcatctatat
    18001 cactttcttg atattcagct cctccgatta aaacagtcat agaatctatc ctatgattat
    18061 tcactaaagc ttttccttct ggttttaaat aattgaacca tcttaaagct tccatttttt
    18121 caaaagatac taatatatct gctccaccaa tttctataac tggagaatga acacaatctc
    18181 catatctaac ttgtgaggaa actgaacctc ctctttgact cattccatgt atttcactca
    18241 tctttacatc atatccagct tccatcaatc ccatagttaa taatttacta gcaagtatag
    18301 ttccttgacc tcctacacct accaaaagta tactcttagt catattattt ttcctcctta
    18361 actattgctt gttttggaca aacttgtgca catactccac atcctacaca ctgatttcta
    18421 tctatatttg ataacttatt ctctttatca aatgatagag ctggacatcc agttttcaaa
    18481 cataatttac atcctataca tttatcatgg tcaactttac acttagtctt aaatggatta
    18541 ttaaattctt ctatatcttc tttagagaat tttttaagta cacatggcca tcttgttatt
    18601 attactgatg gttcatcttc tatagctaaa gcccaatcta gtgcttcatt tacttctttt
    18661 aaattattag ggtttataac tcttacatgt tctattccac aagctttaac taatccttct
    18721 atatcaactt ctttagtttt agcaccttgt aaagtatatc ctgaacctgg gttttcttgg
    18781 tgacctgtca ttccagttat tctattatct aatataaccg aaatagaatt acctctattg
    18841 taaacaacat ctaaaagtcc atttattcct gtatggaaga atgttgagtc tccaagtaca
    18901 cctaccaatc ttttttcatt atcatccttc atattaagaa ctttttgagc tccatgtgca
    18961 gttccaaaag cagaacccat acaaacaaca taatctattc cattatatgg aggagcaaat
    19021 ccaagagtat aacaacctat atctccacca actattaaat tttttctctt tccaagttca
    19081 tagaagaatc ctctatgtgg acatcctgca cagaaacttg gtggtcttgg tgttactaat
    19141 tcagatttgt attctatagt atcatttgtt tttccaaata tagcttttct taaaacatct
    19201 ggtgtcattt ctccatatgg tggtattaca tcttttccaa tacaatctat tccatatgct
    19261 tttatttgtt cttctataaa tgggtcattt tcttctataa catatatttt atcaactttt
    19321 tctgcaaatt cttttatttt ttcataaggt aatggatttg taaatcctag tttcatatat
    19381 gatgcatttt ttccaaaaac ttctttggca aaattacaac acataccaga tgctataaca
    19441 cctatttttg tatcatttat ttcatagtag tttaaatcag tttcatttga gaatttttta
    19501 agaatttcca ttctttcttc aacaacgccc cttcttattt gagcatttgc tggaacagtt
    19561 accattttct tagcattttt aacatactct ttaatttcaa cttcttctct atcatagcac
    19621 tccactaatc cttttgaatg acaaagtctt gttgtaactc tatataaaac tggtgtatcg
    19681 tatttttcac taacttcaaa agcttctttt atcatatctt tagcttcttg actagttgat
    19741 ggttcaaata aaggtatctt tgcaaatttt gcatacatcc tattgtcttg ctcattttga
    19801 gatgaatgca ttcccggctc atctgctgta ataagtacca taccaccatt tacccctgta
    19861 tatgcataag taaatattgg atcagctgca acattcaaac caacatgttt catagaagcc
    19921 attgttcttg ctcctgcaat tgaaccccct atagcagcct caagtgctac cttttcgttt
    19981 ggagcccact ctgcaactat tgcatcttta tatgttgcta tattttctaa tatttctgta
    20041 cttggagtcc ctggatatgc agatgcatat ttaacaccag cctcataggc tccacgtgca
    20101 attgcttcat tacctgtcat caattgtttc atataaaatt ccctccatct acaattatta
    20161 aattcatagt ccacccaatt tgttcaatga aattaattat ttatttttac ttctaaactt
    20221 tcaagtgctt cttttataat acttggtgct ttcttacatt tatcttcaga aactgcacaa
    20281 actgcaaatc ttaagcccat ttttaatgga actgcaaata aattatgttt agtcaattct
    20341 tcacatactt cttttggatt atcacatggt atacttacaa agaaacctgc tatatatgga
    20401 agaatttcta atcctactct ttcagcttct tttacaaata catctgctct tctagtaagc
    20461 atgtttttgt atatttcttt ttcatcctca tattctttga attttttagg gtcatttaca
    20521 atgttagata atactgccat agcacttcta ttacaatttg accaatttgc tctacatgaa
    20581 tgaacacatg agtaatggaa ctcttcagct acatcttcac ttgaagatat acaaattata
    20641 gctcccattc tcataccata agctgtaaat ccttttgaca tactgaatcc aaccaatacg
    20701 agaacgtttt caggcaaatt agaaaatttc ttaaaaaact ttctacatac atcatcatca
    20761 cctgcaaagt caatatatgc tgaatctacg aaaaatatta tctttttgtc tttatctttt
    20821 gcgacttctt ttgataagtc taatatttta tcccactcat catctgcaac actatatcca
    20881 gttggattat gcgctggtgt gtttataatt gtaaagactc taccttgttt ttctgctata
    20941 tttacaaatt tttctttaaa cgactcaaaa ttaaaccttc tattttcatc aaaaagttgg
    21001 taattaacta cttttctatt agcctcttca gctatactta catatggact ccagaaccaa
    21061 tcagatgtta atacctcatc tccttcatta gtatagttcc atacagctaa ttttattgca
    21121 ccacttcctc ctggtgaagc taatactctt atatgtcctt ctggtaagta atctctgaaa
    21181 aatacttttt taactgcttc taagtaatct ggttgacctt caagagctgc atatgcacct
    21241 atctcacagt tatctaatgc tttatattct tcatatacag ttttcattgt tatcaatttt
    21301 cctgaatcat ccattagagc gcctattgtt gcatttatta cattatccat accaaaagcc
    21361 ttctctgctg cttgagctct gccagatatg ctaaatataa catcattttc ttttggccac
    21421 attgcatgct taccaaccat actttgactc ataccatcac ccctaaaatt gtatttactc
    21481 ttacattata tattaagcaa ttactatgcc aataaatccc actttatgta aaattgattt
    21541 tgtttagaat tattattttt ttcattatta aattaagtgt tttaactatt tttcttataa
    21601 atatatatgc ctgtctaaaa aaataaaaat ttgcaaaaaa gtaatgttaa tttttcttaa
    21661 cacttgtttt aaaaaattaa cattacttca aaataattta tatgtaatta tatttaaata
    21721 tttttattgt atttcttcca taaagatgtc ctgcttattc caagaacttg tgcaacttca
    21781 tttttagaaa gtccctgagc aaaaagcata cttattattt tttcttcaac atacttattt
    21841 acttctttta tatctatact tatcttacca tcatttatct tacttatatt tatagattca
    21901 tcttggactt ctacacttgc tgtcgtttca gaattaaatt tgtctacttc agagttagga
    21961 agtaattttt tagttttaaa atctctatct tgtctcaatt taattttacc tgtcacaaca
    22021 tatttttgag ctattgtctt taactctctt acatttccag gccagctata attatatagc
    22081 ttatccattt catctttagt caaaacaaaa ttttcatcta tactgtttat tccattgaga
    22141 ccatcatcct ttaatacttc atttacaaaa ttgttaaaca aaggtattat atcttctctt
    22201 ctgtctctta aaggaggtat atttatctct aaactactca atctataaaa taagtctgct
    22261 ctaaatgtac ccataactat tttttcggtt aaagattcat tcgttgctgc tataatcctt
    22321 atatccagag gtattatata atcagaacca attctcatta tttgacgttc ttctattact
    22381 cttaatagtt ttgtttgaat attaaaggat agactattta tttcatctaa aaaaagagtc
    22441 cctccatgag caagttcaaa tagtcctctt tttccaccct ttctagcacc agtaaatgca
    22501 ccttcttcat agccaaaaag ttcactttca agtaaatttt ctgctatagt agcacaattt
    22561 atagcaacaa aaggcctatc ttttctttta cttatgttgt gtatactatg agctataatt
    22621 tctttaccag acccactttc tccataaagt aaagttgtat aatcactttt tccaattttt
    22681 ttagcttctt caataaattc tttagttaat ttatctttaa ataaaaaatc atcaaaagta
    22741 tatctagcat agagaccttt ttgattcaag tcaaatctta tttttctttc taagttttgt
    22801 agcttggtta tatcttgtat tattcctaaa actccataag tactattatc aacttttatc
    22861 aatgttgtcc tagtattaac aattagatta tttatatttc ttatctttct atcttctacg
    22921 tcctcttttt catgtaaaca atctaacatc cactccattt taggaaatac atctaagatg
    22981 tatttattaa gtgcacattt tctctctact ttaagcatgt tttttgcact ttcattgtac
    23041 aaaattatac tgccattact atctatagca attactccat cttttatacc atcaagtata
    23101 tttcgtaaca cttcattatt aaacttttct tcacctaatg tatctaataa ttttttacaa
    23161 tattctacag cttctcttat agattcatca ctagcagttg caaatacact atcaattcca
    23221 tattgccttg caaaagaaca tgccaaacct cctcctacga ttacaacttc atctttttgg
    23281 tctatatatt ttacaacttt acttcttatc tcatacttag attcaaacca ttcttcagtt
    23341 atttcagtac ttatgacatt tctccagcca acatagtcaa aagaaacttc attgtctcca
    23401 agaatcaata ctactttttt agaatatttc tttgctattt ctattgctct taaaaggtca
    23461 gttgacttca tattcattgg tataacaggt acatttactg tatctattac taagctataa
    23521 cctccacctc taccaattat agcttgggca cctttttcaa caagtatacg accttgattt
    23581 tccattaagt ctaggttaag tatgtcgatt attatagttc cattttctac atcttctcta
    23641 tataattctt ctattctttc ttttaattca atatcagagg ctataattcc tatttttttt
    23701 cgcaacaaaa tcacctcaat tttaaattat aatgtattga tttctacctg ctttttttga
    23761 tttatatagg ttcttatctg catcttcaat taatatttca acatcttttc tactacgaca
    23821 cctttttata cttactccag cacttatagt tatatagttt tttattttag aatatatatg
    23881 ttcaataccc tcaaattcaa gaagtttgaa aactctttta atatataatt ctatattatc
    23941 aatctcttca acaccgattg attctataga atctactaat attaaaattt cttctccccc
    24001 atatctgatt atgtgttcgg taatatctat aaatacactt cttataatat cagttacctt
    24061 aactataact ttatcccctg ctagatgacc ataattatca ttataacttt taaaacaatc
    24121 tatatcgacc agtattgcga ccatattttt gtctctggtt ccattaaata aatccaactg
    24181 ctgttccaaa tatcttctat tatatagttt agtaagtcca tcttttaaaa tatcattttc
    24241 taatttctta ttatcatcca ctagtaatct taaactctct tgtttttgtc tgagttcata
    24301 ttgtttgtga attgcttgag atgttttata ggaattattt ttttcccacc tatcagaata
    24361 ccaatccata cttttataat attctattgc cagtttaaaa tcttttttta gctcatatcc
    24421 atttgctatt agcccatata aatatagata tttttctaag attcaatgaa ctctttttaa
    24481 ctaaatttaa accttttata taacaactta taccttcatc tatattacca aattttatat
    24541 ataaatctcc atacaactta tatatgtaat ctagtttaga attatatata aaaacatctc
    24601 caaaactttt atatatattt tttgataaaa ttaaataggt gtttaccttt tcaaaactac
    24661 aattatcttt ctttaactca atttcagctg aatataaaaa tataaatgaa tataaataat
    24721 ccttatatac accttcaaca actttcatta attttatagt ctttaataaa ataacttctg
    24781 cttctttatt catatcttct tttaaatatg cccatataag gtctattttt atagacactc
    24841 tttcagttac tgatattgag ttattactgt gtgcaacatc taatacatta tttatgtatt
    24901 ttatagcttc tacattattt ccactctcta aataacacaa acttattata catttacaaa
    24961 aagcctctat agagtataaa ttattttctt gagccaattt tagacattca attaagtatt
    25021 ccatactttt tataaagtta ttaaataaat atagataaac tctcccaaat atataatttg
    25081 ccctacttac aagagcttta ttttttaaag ttttaaaaaa agacacttct tctactaaag
    25141 ctattacatc cgttttggtc tgatataaag cacttatttc cacacataaa ctcatattta
    25201 acaataccaa caactcattt aaatgatttt gccttataat tttttttgct gagaaataaa
    25261 aattatttac cttatcatat tttcttatag aaatagctga taaagtaaga taaatataaa
    25321 ttttagatat tatatctgaa tcatctttta atgacaattt tttaatatac tttaatgact
    25381 ctatcaaata gttgtatgat gcttctctat ctttttttat atagtttata agactaatta
    25441 aatataaagt atagactcta ctattatctt ctaattttgt atttaaaagg tctttgtgat
    25501 aatccaagat gtagtttaaa tctgtcaaat ttagatagaa tttagtcaac atatcgtatt
    25561 ctatcttgtt tatatttctc tttaaatctt ctattaaact ccttttcaag taaatcacca
    25621 atcctatttc aacagttaaa aattcttaaa atcatgataa tgcaaacatc tatttctacc
    25681 actttccttt gctttatata aagctttatc agcattttca attatttctt ttatatctat
    25741 actactattt ttacatgtat caatgccaat actaactgtc aaattattat ttaaaaattt
    25801 ctttacaatc ctacataatt ttcttccaaa tattttactt tttttataat ctttacatac
    25861 agataatatc agaaattctt ctccaccata ccttatcata agcatgtctt tttttagata
    25921 tttttttatt attatagtta tatttttaag agcaatatct ccattataat gtccataagt
    25981 atcattgtat tctttaaagt aatctacatc tatcataaat gctgtgcaat attcactaac
    26041 ttcttgtttt tctaaaaaat cgttcaaaaa agttctatta tatgcacttg tcaaatggtc
    26101 aacatacttt tttttatcta aatcaagttt tacttttctc atgttattta tttctttttc
    26161 tatatactca tgtctataat ttaaggaatc tatataattt ctatttcgag cttttatata
    26221 catcattttt aactcataat acattttaaa gtatttcata ctcatttcat aattttgaat
    26281 cttttcaaaa cacaaatata tactcttgta tgctaacttc acataattat aatttttagc
    26341 atttttagat ttttcaagga gttttttatt tgtatttaag gcattgtcat aatatccaaa
    26401 gatataatat atctttgcct ggtgataaat tatttctaaa tcatatatta tatattcttc
    26461 tatatttttt atcatattta atatgttttt agctctatct aaatagcata ttgattgttc
    26521 aaaagatttt tcattacttt ttgcataata catggctaaa cttataaata aatcaaactt
    26581 ctctacttct tttaagctat gatttttaat tttttctaga tatacttcag attttgctaa
    26641 attatttatt gcgacaggat aatttctagt ctttattaat attttagtta tgttgtttga
    26701 tgctctatat ttttgaatta tatttacatt tctatacttt ttattgctta ttacttcatt
    26761 gaaatattcc atggaacatt cataattttc aaacgaacat tcacactcac ctatctttat
    26821 attacatagt gaagatactt gcatatcctt tagtaaatct gcatattttc ttgcttttaa
    26881 aaaatttacc ttagcaatta gagcatcatc caaaaacaaa aaatatattt taccaataat
    26941 aaaaaaatat atttttttac aattaaaatc gttaatttct tttatcttta tatacaacgt
    27001 ttctatttct ttaattatta cagaagattc tatacactta cttgccttaa tgtaaataag
    27061 ttgtgattta aaaataattt cttcaattag aaaattattt tcatcacaaa taagattaaa
    27121 tttatccata tataaatttg ccttatttac atcttcattt tttaaactta aaatagctat
    27181 ttgtgaataa aatcgtgcta aaaatttata atctttgggt aagcatttat caatatattt
    27241 acaggaaatc aaaatatact ctttcatttt ttcactaaac ccttgaatac catataataa
    27301 agctagcaaa aaatttgatt ttaataacat taaactattt tgttcagttt tatagcaatc
    27361 aattaaattc tttactacat caattttatt ttcaataaaa tcgttattag tcaataatct
    27421 ttcattcaag gttagtttaa acattgaatc taaaattgta tcatctatct cttccaaata
    27481 tttactatat attgtaactc acctccgact tacattatct cataaattta gtaattttcc
    27541 atactatttt tgaaaaaagt tgatgtctca atttagaaat ttctcttaaa ttaagacata
    27601 ctttacacta aactatacaa ttttctatct gaactatata atcctgacaa ttttcgcaaa
    27661 ttatcacact tacaatataa ctattatcaa ctttatttgc ccttgatagt ttattaaaag
    27721 atagtccttt ataatcaaca gatatattgc ttattacata ttctccatct ttactgaaaa
    27781 tataaaagtg atattctaaa aaaggaaaga attttttctt ttcttttact ccttctaaac
    27841 ataatggaca tgcttcttga taatattcta catcaaaatc aacttcttct gcttcattaa
    27901 gtatatcaat tatttttgaa atacttactc cttgaactgg attttcttca tcatttatta
    27961 aagcactaca gttctcctct tttaataaat attccttatc atcatatata aacttatata
    28021 aactcatatt atatcctcct tataattatt aatctccaag ttattctacc atttaaataa
    28081 agttaataaa agtaattgtt tataatcttt ataattaagt tatgtataca tatattccta
    28141 tgcgctatct taattaatga ttttaaaatc caagtttgca ctttgaacat ccagtttccc
    28201 aaaatctatt gtcaaaactc actttagtta ttaatataat gatataaata tataactgca
    28261 tagatgttac acacttaact atttaatatg atacgatttg gtatctaaat ttatatattg
    28321 tatatagggg gagatatata tgtataaaga taaaagtgat gaatgtatac acttaatgac
    28381 tgcatacatc gatagtataa gtggatatta ttcatttatt gacacacaat tagaggattt
    28441 tatgatgaaa tatggtgaaa acattgttga ttcaaattta cacagtataa tgatgttgct
    28501 ttgtaaatgg ggattatctt agcatttcaa gaaacttttt tgtatattgt attctatatt
    28561 atttattcat taacaaaaaa gattgacata aacttaaaag ctatatatag atttctttaa
    28621 aaatctatat atagcttttt taattttttc aaaatgtata cttaaataag ttagtttttt
    28681 tcaaaatttg actatctaat atgatataat ttaactatct acaacaataa aaaatatcta
    28741 atatgtatta tctagtttag ctcatcataa taacatccct atatttacaa ttaagatatt
    28801 ttcaatatag taatactcaa ttttaatatt ataaaaagga gagattttgt atggattaca
    28861 atgacttact aaaatcagct agagagaact ttaatggaac atgtaaagta tgtaaaattt
    28921 gtaatggatt agcttgtgct ggagatgttc ctggtatggg tggtaaagga agtggttcgt
    28981 ctttcattga aaatagaaaa agtctagaaa aaataaagat aaatatgaga gtcatacata
    29041 atgtttcaaa gccagataca tccatagaat tatttggtag aaaaatgagt tctcctatat
    29101 tcgctgcacc tgtatcaggt actatattga acatgggtgg taaagtttcc gaaaaagaat
    29161 acatagagcc tgttgttaga ggttgttcta attctggaat ttatgctatg gttggcgata
    29221 ctaatgtaga tactttttta ctggataatt tagatgtatt gaaggataat aggggaaatg
    29281 gaattgtatt tataaagcct tggaataatt caaagataat agataaaatt agattgtctg
    29341 aagaagcagg tgcttttgca gttggtgtag accttgatgc ttgtggactt attaataatc
    29401 aatttcaaga aaatcctttt tctcctaaaa caattgatga gattagagaa ttggttgaat
    29461 ccactagatt accttttatt ataaaaggaa ttatgactgt agatgatgct ttgatggctg
    29521 tagagtctgg tgctagtgcc attattgttt caaatcatgg tggaagagta cttgattata
    29581 ctcctggtac ttgtgaggta cttccagata tagctaagtc tgtaaaggga aagataacaa
    29641 tacttgttga tggtggagtt aggactggtg ttgatgtagt taagatgctt ggattgggtg
    29701 ctgatgctgt tttgatggga agaccttttg ttactgcttc ttttggcggt ggtcttgatg
    29761 gtgtggagtt ttttatagag aaggttagga atgagttatg tgagactatg attttgactg
    29821 gttgtcaaaa tgttaaggat atagatggta gagttatatg gaatttattg taaaatagtt
    29881 ttataacatt tatatttaaa tagtgtctca tttaaattga aaaatcatat tgagacacta
    29941 tttaatatat ttttagattt taatcctatg aaagagtagg gtcagttagt ctacccaatt
    30001 tatcccaagt agctggacca attattccat ctgcagacaa accgtatttt ttttggaaac
    30061 ttacaactgc attatatgtc ttacttccaa aagaactatc tatacctaca ttataaccaa
    30121 gtaaagttaa catgatttgt gcagctttta cttcttttcc gctattaccc tttcgtataa
    30181 cttttgttgt acttatagtc aaatactcat tactcataat tgttatagtt ttagcaccta
    30241 tattataaga aacagtataa cttgcataaa cacctttact attgtcctca ttcattagat
    30301 tagctgattc tggaacaaat atctcatcat ctggcttagg gtctatattt atctcttcac
    30361 aattttgttt atgttcatct cttatttctt gtggcgcatt ttcatatgta tatatatacc
    30421 cagaaagagt aaaagaatct aggtttttct ctggaacagt ctctttactt cctcctttct
    30481 ctttacttgt gtcagcaaat gccacacttg taaaagtaag caataagcaa cacataatag
    30541 ctactatttt tcttaatttt gttaacataa aatcctcctt ataattattt tattaattga
    30601 ttataatctt tcatatccat ttctattatg tcatagtctc taggtatttt accttctaat
    30661 tcgcatttat cttcataaaa tttcctaatt tcatttggtg catctttata ataaatattt
    30721 ataattttat cagtcgtctt attcaataaa aattcattaa tattgttatt cattccatta
    30781 ttaccctcat caattctaac ttgagcatca acttcaacaa aagatgttat agaagtcata
    30841 aatactaaaa cagcaattag tattccactt ttgcctttct ttttaacaat actctcaagt
    30901 ctcttttcca aatttgtctt atcaaactcg gttgtcaaaa tcttactatt ataattttta
    30961 ctaaacttaa ttgactttat aagtgctagt gcatattctt ttttatcttt taaagaacat
    31021 tctaccaaaa cactttcatc acaacataat tcacaatcta gatttacttt attgctcatt
    31081 acatatacaa gtggattaaa ccaatatatt attctaacag acaaaactaa aaactttatt
    31141 aaaatatctt tatttttaaa atgcattaat tcatgtttca aaatccaagt tagttcatct
    31201 ttactataag gatagtctgg taagaatatg taagatttaa ataatcccat acctgctgga
    31261 cttatcaatt catcagaacc tcttaattca attttctttt ttatatttaa ttcttttaac
    31321 aagtttttgt agatacagtt tatatcatta tcttcaatat cataagatac atcaacaact
    31381 agatttttaa atcttgtata ctttataaat gtataaactg caatgactat agttacaaat
    31441 aaccataaat aagctacata cagcataaga ttattatctg tagaaatatc aatttgtgta
    31501 atattatttc ctaatgcatt ttctttgggt tcttttactt catacactat tgtataatta
    31561 aatagaaaaa gtaacattct aaagactatt atcaaccaaa tataataatt gaatctctta
    31621 ctaaacttct taaataaata tcttttcaaa aataataata gacaaatacc aagactacat
    31681 acaatagttg tctttataag attttcaaaa acatatagca tataattatt catcctttaa
    31741 attatcaaaa taactttcta gaaaatctag cttatcttta cctatatttt catcatcatg
    31801 taatgctgaa attaagcttc ttaaagagtc atcatgtata ttacttagga aatcctttgt
    31861 ctcaaaactt aaataatctt tgtgttttac tcttattgtg taatgtgtat atctgtctat
    31921 tttttgagca tctaagaatc ctctttttac cagcctagat aaaagtgtca gtgtggtggt
    31981 ttgtttccag ccatattttt gttccatagc ttcaataaca tcttttgatg tcactgtaac
    32041 atctactttc caaatgaatt tcattacttt tagttctgct tgtggtattt ttcttatcat
    32101 tatttacacc tccctcaaaa ctcctgctac cttaattcta caacatgtga attttttagt
    32161 caaacacttt atgaaaaaaa tgtgaatttt tgtacttttt gttaaatttc taattttttt
    32221 acaatattaa aagttagatt ttaaaatata tctatttaaa tattttactt tttacatatt
    32281 taattacaat agtgtagaat gttatttaca cattaaaact atttaataaa aattcctata
    32341 ttttcacaca aggaatatta taatactttt ttttaaaata aactagagta atatc
//
LOCUS       DAELTA010000028        31814 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.028, whole genome shotgun sequence.
ACCESSION   DAELTA010000028 DAELTA010000000
VERSION     DAELTA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.028"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            230..547
                     /gene="flgB"
                     /locus_tag="KPG13_001117"
     CDS             230..547
                     /gene="flgB"
                     /locus_tag="KPG13_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG0984855.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            555..959
                     /gene="flgC"
                     /locus_tag="KPG13_001118"
     CDS             555..959
                     /gene="flgC"
                     /locus_tag="KPG13_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG0984856.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            969..1286
                     /gene="fliE"
                     /locus_tag="KPG13_001119"
     CDS             969..1286
                     /gene="fliE"
                     /locus_tag="KPG13_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG0984857.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1311..2861
                     /gene="fliF"
                     /locus_tag="KPG13_001120"
     CDS             1311..2861
                     /gene="fliF"
                     /locus_tag="KPG13_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG0984858.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2874..3944
                     /gene="fliG"
                     /locus_tag="KPG13_001121"
     CDS             2874..3944
                     /gene="fliG"
                     /locus_tag="KPG13_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG0984859.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3937..4668
                     /locus_tag="KPG13_001122"
     CDS             3937..4668
                     /locus_tag="KPG13_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG0984860.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4672..5991
                     /gene="fliI"
                     /locus_tag="KPG13_001123"
     CDS             4672..5991
                     /gene="fliI"
                     /locus_tag="KPG13_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG0984861.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6023..6469
                     /gene="fliJ"
                     /locus_tag="KPG13_001124"
     CDS             6023..6469
                     /gene="fliJ"
                     /locus_tag="KPG13_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG0984862.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6497..7702
                     /locus_tag="KPG13_001125"
     CDS             6497..7702
                     /locus_tag="KPG13_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG0984863.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7720..8328
                     /locus_tag="KPG13_001126"
     CDS             7720..8328
                     /locus_tag="KPG13_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG0984864.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8355..9338
                     /locus_tag="KPG13_001127"
     CDS             8355..9338
                     /locus_tag="KPG13_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG0984865.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9351..9554
                     /locus_tag="KPG13_001128"
     CDS             9351..9554
                     /locus_tag="KPG13_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG0984866.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9554..10372
                     /locus_tag="KPG13_001129"
     CDS             9554..10372
                     /locus_tag="KPG13_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG0984867.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10362..11057
                     /locus_tag="KPG13_001130"
     CDS             10362..11057
                     /locus_tag="KPG13_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0984868.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11069..11512
                     /locus_tag="KPG13_001131"
     CDS             11069..11512
                     /locus_tag="KPG13_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG0984869.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11527..11901
                     /locus_tag="KPG13_001132"
     CDS             11527..11901
                     /locus_tag="KPG13_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG0984870.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11902..12567
                     /gene="fliP"
                     /locus_tag="KPG13_001133"
     CDS             11902..12567
                     /gene="fliP"
                     /locus_tag="KPG13_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG0984871.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12580..12849
                     /gene="fliQ"
                     /locus_tag="KPG13_001134"
     CDS             12580..12849
                     /gene="fliQ"
                     /locus_tag="KPG13_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG0984872.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12861..14675
                     /locus_tag="KPG13_001135"
     CDS             12861..14675
                     /locus_tag="KPG13_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG0984873.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14684..16759
                     /gene="flhA"
                     /locus_tag="KPG13_001136"
     CDS             14684..16759
                     /gene="flhA"
                     /locus_tag="KPG13_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG0984874.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16734..17750
                     /gene="flhF"
                     /locus_tag="KPG13_001137"
     CDS             16734..17750
                     /gene="flhF"
                     /locus_tag="KPG13_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG0984875.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17747..18625
                     /locus_tag="KPG13_001138"
     CDS             17747..18625
                     /locus_tag="KPG13_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG0984876.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18639..19340
                     /locus_tag="KPG13_001139"
     CDS             18639..19340
                     /locus_tag="KPG13_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG0984877.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19356..19778
                     /locus_tag="KPG13_001140"
     CDS             19356..19778
                     /locus_tag="KPG13_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0984878.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            19763..19948
                     /locus_tag="KPG13_001141"
     CDS             19763..19948
                     /locus_tag="KPG13_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG0984879.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19989..20762
                     /gene="flgG"
                     /locus_tag="KPG13_001142"
     CDS             19989..20762
                     /gene="flgG"
                     /locus_tag="KPG13_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0984880.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20783..21544
                     /gene="flgG"
                     /locus_tag="KPG13_001143"
     CDS             20783..21544
                     /gene="flgG"
                     /locus_tag="KPG13_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG0984881.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21567..22448
                     /locus_tag="KPG13_001144"
     CDS             21567..22448
                     /locus_tag="KPG13_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0984882.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22451..22825
                     /locus_tag="KPG13_001145"
     CDS             22451..22825
                     /locus_tag="KPG13_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG0984883.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22857..24260
                     /locus_tag="KPG13_001146"
     CDS             22857..24260
                     /locus_tag="KPG13_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0984884.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24376..26313
                     /gene="htpG"
                     /locus_tag="KPG13_001147"
     CDS             24376..26313
                     /gene="htpG"
                     /locus_tag="KPG13_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG0984885.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26701..27831
                     /locus_tag="KPG13_001148"
     CDS             26701..27831
                     /locus_tag="KPG13_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0984886.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28058..29080
                     /gene="splB"
                     /locus_tag="KPG13_001149"
     CDS             28058..29080
                     /gene="splB"
                     /locus_tag="KPG13_001149"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG0984887.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29262..30173
                     /locus_tag="KPG13_001150"
     CDS             29262..30173
                     /locus_tag="KPG13_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0984888.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30188..30604
                     /locus_tag="KPG13_001151"
     CDS             30188..30604
                     /locus_tag="KPG13_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0984889.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30704..31096)
                     /locus_tag="KPG13_001152"
     CDS             complement(30704..31096)
                     /locus_tag="KPG13_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984890.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31237..31632
                     /locus_tag="KPG13_001153"
     CDS             31237..31632
                     /locus_tag="KPG13_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0984891.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13051 a         3381 c         5836 g         9546 t
ORIGIN      
        1 gacttcttta tttatttaaa ttaatcgcat ttccattaca ttaatacaat aatgtatcaa
       61 tttagtaaga aaactaatat ttttttaatg aagtcccatg aaaatataaa aaaattttaa
      121 tatgtattta aatatttctt cgaaaagttg ctcttttttg taatatagca acttcatttt
      181 ttttattaat aaacaaaata gataaaatat attttgagga ggggtgagtg tgaatacata
      241 tgacattata aaaatgggat tagatgcaac taatctaaga agtcagacaa tagcaaataa
      301 tatagcaaat ataaatactg aaggatataa aagacattat ataacttttg aagaaacact
      361 aaatgatgct atgcctaaaa tagaagaaaa aacagacaat tctaaaggtt taagagtaga
      421 tggtaataat gtagacatag aagaagaaaa agtaaatcaa gcaatggcaa cattacaata
      481 taatgctttg ataagttttg caaatggaaa aatagcaatg accaaatcaa taataagtgg
      541 gaggtaatta gaagatgagt gtgttcagtg gaatgagaat atctgctagt ggattaacag
      601 cagaaagttt gaaaatggat atcatatcat ccaatgtagc taatataaat gctacaagag
      661 gtccaaatgg agaagcatat aggagaaaag ttgtttcatt tgaagaaaac tatgataata
      721 aattaggtat gctaggagta aaagttacat ctgttgaaga tgataaatca cctcttaaag
      781 aagtatatga cccatctcat ccagatgcaa agccaaatgg atatgtagaa tatccaaatg
      841 taaatatatt ggtagaaatg acagatttaa taacagcttc acgagcatat gattctaacg
      901 tagatacatt gaatgctcag aagtctatga tatcaaaagc tttggaaata ggaaagtagg
      961 gataaattat gccaattaca caaattaaca atttaatgca aggtgcaaag ttagataatt
     1021 tgattggaga agcaaagcca ttaactctta ataataaaga agaaaaaaag aattttgaag
     1081 atgtaataat agatactata gataaagtta attttcaaca agtaacagca gatgatatga
     1141 aagaaaaatt tataaaaggc gaagatgttt ctatgcatga agttatgtta ataggtcaag
     1201 aagcacaact atctatgcaa tatttaatag aagttagaaa taagattcat gatgcatatc
     1261 aagaaatgaa taaggtgcaa atttagcctt ttgaacagga gtaataatca atgataacca
     1321 gttttaaaaa taagattaag gagcaagttg ctaaatctag gacaaagata agttctttaa
     1381 aaaagggaac taaaatagca ttaggtatag gggttattgc tatatgttta gcgctagtat
     1441 ttacagcaat atctgcgagc aaaaataagt atgcagtctt attttcagaa ctagatgctc
     1501 aagatgctgc agatatatca gcagagctag agtctaaaaa gatagatact aaggtaaaag
     1561 gaagtacgat ttatgtacct agaacagaag ttgataagtt aaggctggag ttagcgccaa
     1621 gtcttacagg tggttctaaa ggatttgcac taatggatga tagttcatca atagggctta
     1681 ctgatgaaga atttcagatt aagaaacaaa gaatgataca aggtgaaata gaaaaaacaa
     1741 taaagagttt tccacaagtg gaagacacta gagtacatat aacatttgga gagtcatcag
     1801 tatttgagaa ggaggctgtt ccagggagtg cagcagttta tttgatgcta aaaccaggga
     1861 cgactttaga aaaaaaccaa ataaagtctg ttatggcact tatatcagga agcgttacaa
     1921 atataccaga gaaaaatgtt caggttattg ataacaaaat gaatttatta tctgaaggta
     1981 ttttggagga tgaaaatgtt gatgaaaata atcaaccaat aaatagtggc attagtgttg
     2041 ataaaaataa ggcatctgaa caagcattga ataaacaact agaaaagtct attttagaaa
     2101 tgctagaacc gatatttggt aagggaaagg tgaaagctac agtaaatgct gatttaagtt
     2161 ttgatacagc tgaaaaaact gagataaaaa ttgaccctga taaagttgca ataaaggaga
     2221 caaggtcgaa aaattcatct aaatctgctg caaataatgt tcaaggtgta gatgctaaca
     2281 tgaataacca aggaaataac aatggtacta atacagaaga tagtttagat gagagttttg
     2341 agtatgaaac aggtaagatt gaaactcaca ctatagttgc gcctggagaa cttaatagat
     2401 tgacagcttc tgttgcaata gatggaaaaa tagctaaagg tgttcaagct gatgtagagg
     2461 atattgttaa taatgctata ggtatggatg gagcgagggg agatactctt agcgttgttt
     2521 caatggtatt tgacccagaa ggtaagaaag aggctaaaca agagctggaa gagattaaaa
     2581 aagaaggatt taaaaagaac ataataaaag cagttattgg agtagtagca gtaatagcag
     2641 ttggagctat tggatactta atatataaga aaagaagtga acgagaagat atggattatg
     2701 aagatgaaga ttatatttct aaatctgatt ctgttgatag gaaggataat cctctattag
     2761 gagatgaccc agaactgaca ttagaggaag ctgttaaaat gtatgcagaa gaaaagccag
     2821 accaagtaac agagataata aagacttggt taaatgagta agggggatag aaagtgtcca
     2881 cggaaattac aaatgttaat gcgtttgact taagtgatat aaatgaaagt gataagaatg
     2941 agaaggtaga aggtgagggt ttaaaaaaag ctgctgtatt attaatgaca ttaggcccaa
     3001 atgtgtctag tgatataata aaaagcttgc ctgataaaca agttcaaaaa ataggtttgg
     3061 aaattgcaaa tatttcttca atagcttcaa aagaaagaag agaaatctta aaagagtttg
     3121 tagaaataaa tagggcaaaa gattatgtta tagaaggtgg gcttgaatat gcaagagagt
     3181 tattaagtaa tgctttagga aatgttaaag caaacaaatt acttgaaggt atatcaataa
     3241 atacaagtac aaagcctttt aaacatatta taaaactaga tgtaaagaag atattagaaa
     3301 tacttaggaa tgaaagtgca caaactatag ccgttttact tgcacatatg gaaccagaaa
     3361 atggagcaga agttttatca tacttagatt ttgatttaca aaatgaagta gctatgagaa
     3421 taagctcaat gggttcaata tctccagaag taataaaggt aattgaaagt tcattagaag
     3481 ataaattaca ttcaataagt ggtgatgatt ttaatgatta tggaggggtg tctaccttag
     3541 ttcaaatatt gattaattct gatagaaaaa cagaaaagaa tattttggga actattgaag
     3601 aacaaaaccc ggatttagca gctcaaatta aatctcatat atttgtcttt gaagatattg
     3661 tcaagttaga tgatataagc atacaaaaag tgttaaaaga agtgactatg aaagatttag
     3721 tatatgcctt aaaaggtgta gatgatgaga tttcagatgc tatatataga aatcaatctt
     3781 ctagagcagc agatgcactt aaagaagaaa tggaaatggt tggtgttata aaggttacac
     3841 aagttgaggc ttctcaacaa aagatagcta atatcgtaag aaaacttgaa agagaaggtg
     3901 ccataactat tgtaaggaat tttggtggtg actttgttga ataataagat tattaaaagt
     3961 aaaaaagcat tatataaagg agtgctagat ttaaccataa tagataaagt tagagtggga
     4021 gaatcaaatg taaataaaga aaatgattgt agatttaggg atgaggtaat aaaagaagct
     4081 atagaatctg ctgagataag aaagaatgag atattagaag aagcaaaaat gaatgcacaa
     4141 aatatagaaa agcaagcata tgaaaaaggg tatagtcaag gtcaaaaaaa tggatatgaa
     4201 gatggatata aagagtcata tgatgaatat acccaaaaag ctaaagatga agcagatttt
     4261 ataaaaaatc aggcagactt aatactatta caagctaatg agaagatgac tgagtacata
     4321 aaagaaaaaa ataaagagat tgtaagactt gctattaata tggctagttc tgttctaaaa
     4381 aagcatttcg aagatgaaga atctatgagt gatttgttaa agcaaattat tcaagattat
     4441 gaaggaagta gttcatttat agtaagatgt aattctttct ataaagataa tatagagaaa
     4501 gagtttgaat tattcaaaga ggaaacatca ctaaaatcaa gtgtatttgt tattgcagat
     4561 gatagtatag ataaaggcaa tgctattatc caaaaggaaa atggaagaat aatagttggt
     4621 attgattgtg ctatggaaaa actaaaagaa gaattgcttg gtgaataaag tatgtataaa
     4681 actgatatat ctatagattt tgacaaaata aataagaaaa tagaaaattt agaactcact
     4741 atatcagaag gtagagtaaa gaagataata ggacttactg tagaagttga aggaataaaa
     4801 gcttttgttg gggagttatg cgttatatac aaccaagtta ataaaccagt gaattgtgaa
     4861 gtagtaggtt ttaaagataa agaagtcata ttaatggcgt tgggagaatt aacattgata
     4921 gcacctggtt gtaaagttat ttctaagggg atacctttaa gtgttatgtg tagtgataat
     4981 ttactaggta aagtacttga tggtcttgga aatcctatag acaactcaga tgctgtttta
     5041 ggagatagat acaacctgaa caatgaacct cctgacccaa tgaagagaaa aaagatacga
     5101 aatataatgg aaacaggtgt aagagcaata gatgcattta ctacttgtgg tgaaggacag
     5161 agaatcggca tatttgcagg aagtggagta ggtaagagta cgactctagg tatgattgct
     5221 aggaatgcaa aagcagatgt aaatgtaatt gctcttattg gagaaagagg aagagaagta
     5281 ctagatttta ttgataaaga tttaggtgaa gagggaatga aaaaatctgt tgttgtgtgt
     5341 gcaacatcag ataaagcccc tctagttagg ttaaaaggtg ctcttactgc tactgcaata
     5401 gcagaatatt ttagagacca aggcaaaaaa gtaatactta tgatggattc agtaactaga
     5461 tttgctatgg cacaaagaga agttggtgta gcaataggag agccaccagc tcaaaaagga
     5521 tatacaccat ctgtatttgc aatgttgcca aaacttatgg agagaacagg gacatcagat
     5581 aaaggttcta taactgcatt ttatacagta ttagttgatg gagatgactt taatgagcca
     5641 atagcagata caacaagagg gatacttgat ggtcatatag ttttatctag agacttggca
     5701 aataagaatc actatccttc tatagatgta ttaaacagtt taagtaggct tatgaatgaa
     5761 attgcttcaa aagaagacat aaaaatagcg tcttttgcaa gagacatgct tgctgagtat
     5821 agagaagcag aagatttgat aaatataggt gcatatgctt caggtacaaa caaaaaaata
     5881 gatgaagcaa tctattatca tgagcatatt attaattttt taaaacaagg aataaatgaa
     5941 aagagttctt ttaatgaaac tataagtagt ttaagaagaa tctttgaata aacaatttaa
     6001 aaaaatattt gaataggttg tgatggaatt ggataaaaat tttaagttta gattacagaa
     6061 agttttagac ttaaaaataa aagatgaaga agagataaaa atggaatttg ctaagattca
     6121 acaaaaaaaa atagatatag aaactaattt agaaaactta gaaagtaatt acagcaaata
     6181 ttctataagt aaaaataatg ataatgttca aaatcaaaaa ataactatta actatttgct
     6241 agctttaaac aactctataa tggatttatc agaagaactg gataagtcga caaatgaact
     6301 tgaaaaagct agaaaacagc ttataagcaa acagatagag agaaagtcat tagaaaaatt
     6361 aaaagaaaaa aaatatggac agtattataa agaagaacag ttaaaagaac aaagcactaa
     6421 tgatgagttt gcaagcatga gttatttgag aaatagacaa gttctataaa ataataggaa
     6481 gtgaggatat tattgtatga agatacaagc agattcttat aatattatta aagatattgg
     6541 gaatagaaaa aactcatcta agaataaaaa tataaaaaat aataaatttg aagataatct
     6601 atataagaca acagaccata aaaatgttaa agatagaaga gaaacaagta agcaaaatgt
     6661 tagttcagat agaccgggaa atagtgataa tgagtgtcat ttagatgaag ataaaagtaa
     6721 tcacttagaa gatgaaaata catatgaaaa agattgtagt aagaataatg aagctttaca
     6781 gcaattactg aatcttttaa aagagaatta taaaaaagat aaagatataa acactatatt
     6841 gaataatata aaaaaactaa gtatatcaga tgaattaaaa caagaactta agcttaataa
     6901 aaatatattg aataaaattg caaatcaaaa tttagagtta aatggagcta atacaaataa
     6961 ccctaatgat atagaaaatt tagacataat gaagttaaga gaacttttag aaaataatgc
     7021 tagtgaagat attcaagtaa ttagtaaaac tacagaaaat gatactaaaa gcatagactt
     7081 atacagtttt gatagtaata gaatggatac tcttaactca aataaacaga aagatgatag
     7141 ttctgatatt ttggaaaaac tagcagggtt aaatggaaat aagagtggaa attttggaca
     7201 agtaataaat aaaagcgttg atatgaataa aattaaaaat gataatgtta atttaaaaca
     7261 gatagaatct aatgtaatgg atgattctat aaaagctatt aagcatatga aaacaaatga
     7321 tatacaagaa ttgacaatta aattaagacc aaaagaatta ggagatatga acatacaatt
     7381 attaaaagat ggtgaaagta tgagagctgt tgtgactgtt ttcaataaag atgtctttga
     7441 ctctataaat aaaaacatag cagatttaaa gcaacatctt gaacttacaa atgtaaatat
     7501 aaaagatgta tcagtgcaaa tgcatagcga taatacaaat acatctgata cctttgataa
     7561 agcatttgaa caacagaata gacaaaataa ccaagaaaac atgagtaatc aaaatacaaa
     7621 tgataagcgt aacaatgtag tcatagaaga tgagattaaa gaaaatatta ttgatgatga
     7681 tagagtagat ttactagctt agtaaggggg aagtattgca tgtacggaat agcaacggct
     7741 ttggcaggaa ctcaggtaaa ctataggtca aataacttaa gccaacttga aagaaatatt
     7801 aatgatattg ccaacaaaca agttagaagt aaaggaaatc agacagtaac agacaatggg
     7861 actccaatta taggacaagg tgatgatgct gataaagact tgtttatgaa gattcttgta
     7921 gcacagatgg gtaatcaaga cccaatgaat ccacaagacc caacggagta tgtatcacaa
     7981 ttagcacagt ttgcaacttt agaaaaaatg tcagctatga atgataagct agatatactt
     8041 ttagccttaa gtaactctac tctaataaac tctgctttga gtatggcaac atcattaatt
     8101 ggtaaagaag tagaagtatc tggggaagaa gatgaagatg aaaacattgt aggagtagta
     8161 aagtccacat acataaaaga tggggaagta cacttagaat tagaagtaga tggaagtgac
     8221 aaaactgtag atgttaaata tgaaaaatta cttaaaatag gagaggtaga ggcaaattca
     8281 ggagaagaaa atgactcttc agaacaagaa tctccagaag aagtataata ttaaatggga
     8341 aaagaggtaa atttatgata aaagcaatgt attcaggtgt aagtggaatg aaagcaaatc
     8401 aaacaaaact agatgtaatc ggtaacaatg tagccaacgc tggaactact tcttttaaga
     8461 aaagttcagc tagaataaca gattcatttt atcaaactgt actatatgca agtgctccaa
     8521 cagcggcatt agggggaaca aacttaggac aagtaggtgt aggtagtaag atatcaagta
     8581 taaacaaaga tatgacacaa ggaaatgtac aaccaactaa tagaccatct gatttaatga
     8641 tagatggaga tggatatctt ccagtagtaa gacaaggaac agttatgtat acgagagatg
     8701 gttcttttaa tttagataca ggatttaaag cagctgatgg tgctttgaag aatattaaag
     8761 gtggaagact tgtaaatggt gatggatata tgttacaagg tatgatttat gatggggaat
     8821 ataaagctga tactggagaa tttgttaaaa agggtgatgc taaaggaaaa gagcctattg
     8881 taataccact tcaaagtata agtcctaagg atggaacagt tcaaaatgta ctagagtata
     8941 atatatcaaa ggatggaaca gtagaatttt tattgagtga tggtcaaaga actcaatttg
     9001 tctatgatat acctgatggt gctaaggtag gagatacacc tgctggaata gataaattaa
     9061 aaggtacaat acaaaaagtg caaacatatg cattccaaaa tccagcagga ctagatgcag
     9121 aaggaggaaa cttgttcaag ccatcagcaa actcaggaga cccacaaata gcaggagcta
     9181 caggcacaat aatacaagga gctatagaag cttccaatgt agatatagca gaagaattta
     9241 cagaaatgat aatagcaagt aggtctttcc aagcaaacag taaaataata tcgacatcag
     9301 acgaaatact tcaagaaata ataaatctta agagatagta ggtacatatt atgataagag
     9361 taacggattt aaacggaaga aaatacatat taaattcaga tcttatagtg agaattgact
     9421 ccgtgccaga aagcaggatt ctccttacaa caggagaaaa aaatcttgta aaagagtcag
     9481 tagatgagat agtagataaa gtaataaagt ataaaagaaa aataaatgta ggttatatta
     9541 ggagtgaaaa ataatgaaaa aaacagattc actaacattc attggactaa tattatctac
     9601 agtattagtt ttatttggag cagtaaaagg aagtagttca ggattgaaaa gttttttcga
     9661 ttttgcatct atattaatca cagtatttgg gtcatttgga gctttgatga ttacatttac
     9721 tattgacgat ataaaactta taaaagatgc tctacaatac tcattcaaga caatgaatat
     9781 atcgaaatta gacttattaa atcaatttaa aacactttct aaaaaagcaa gaagagaagg
     9841 acttttatct atagaaagtg atgtaatgaa aatacaagac ccatttatga aaaaaggatt
     9901 agagttatgt attgatggtc ttgaaataaa tgaaataagg tctatactag agctcgaaat
     9961 agattctgtt gaagaaacca ataacagata ttctaaacta tttaaaactt gggggacttt
    10021 cgcacctgca tttggtatgt tgggaactct aataggttta atacaaatgt tatccgacct
    10081 tacatctcca gatttaatag catctggtat gggaaaagca ttaattacga cattttatgg
    10141 gtctttactt gccaatatcg ctttgaaccc aattgcatat aatatagatg agaagactga
    10201 aaaagaaatt tatgtaaagg aaatgatgct ggaggggata ataagcatac aatctggaga
    10261 gagcagcata gtagttgagg aacgtttagc tacatacctt tcaaataatg agaagttaga
    10321 aattatgaaa tccaataaaa atacagaaag agcaatgagt aatggcgcgt aaaaaaaata
    10381 agaaaaagga tgagataaat ccagatgcat ggttggctac ttatgcagat acgataacac
    10441 tgattcttac attttttgtg ttattgtatg ctacatcagt tgtagatatg gaaaaattta
    10501 aaaatatagc agatgcgtta aaaggtcagt tttctgggac aagtatgttt agtacaggtt
    10561 caagtacaag tgagaaatct ccaatagaga gtgttttagt ggataaaaat gataagtcaa
    10621 atttagaaca ggatttaaaa gataaagtta ataattctaa cttacaaaat agtgtaacag
    10681 tcaaagatga tagtagaggg attttactag agctagatga ttcaatttta tttgactctg
    10741 ggatttctga attaaaagaa gatagtaaag ccactcttaa taaagtatac gatatgataa
    10801 agatgatgaa aaataagata gttatagaag gccatactga taatgtacct gtaaaatcaa
    10861 gtacgcatga atccaattgg gatttatctt ctagtagagc agtaagtgtt gtaagatact
    10921 ttgtagaaga aaagggcatg gaccctaaac tattttcggc aacaggatgt ggagagtacc
    10981 aacctttggt atcaaatgaa acagcaaagg gaagagctga gaatagaaga gttaatatac
    11041 ttataactat tgactaggag gaagaggttt gttcaaaaat aaaaaaatta ttataattat
    11101 tttgatactg attattttct cagcaggagc aggttttttt atattaggag aaggtggaaa
    11161 taaacaaatt gacaagattg caggtttgtt tattccaaca gggaaaggca aagatgaagt
    11221 aaagaccgaa aatcttgact tagaagagat ggtattgaag ctagctgatg aaggtgtaag
    11281 atatttaaaa gtatcaataa ctgtatctta tgattcttca tataaagata tagaaaaaaa
    11341 tacgccttta attagggata atattataaa ttgttttatg gcaaaaaggg cagatgattt
    11401 tactgctgat aatttaaaga acataaaaga agaaattaaa aatacattaa atatacaact
    11461 aggtgaaaat gtaattcaaa atatatattt cacaaatata gtagtgcaat aaaaggatgg
    11521 taagacatga tactttattt actaaaaata ttaatatgta tcccaataat tttaatattg
    11581 atagtcttat ctttaaaaat tagccaagga actttattaa aaaaatataa tgataaatat
    11641 ataaaaatac tagatattgt aaatataagt aaaaacaata gcataataat tttaaaaata
    11701 ggtgaagaag gatgcattat ggcatcttct tcttctagca tgagtaaaat aaaagatttg
    11761 accaaagaag aaatattaag aatagaaaac gacattaaag aaagaaattt taaaattcaa
    11821 gattttaaaa tggaaagaat taaaaagaaa tatgatttca gtagatatat tggaaaattt
    11881 acaaaaaagg aagataagta aatggaacta ttatcaaaat tacctttggc tgatgtacca
    11941 tatacaagtt caatgcaatt gtttttattt ctaactgcat taatgttttt accatttgta
    12001 atgttcatga tgacaagctt tgtcagaata gttataagtt tatcattttt gaaatcagca
    12061 ttaggagcac agcaagcaat tccaagtcaa attttggtag ggcttgcaat agtattgaca
    12121 atatttataa tgagaccagt attaaatgag ataaacgaaa aggcattaca accatatatg
    12181 aaagaagaag ttactatgga agaagctatg aaggaagcag aaggacctat taaagaattt
    12241 ttattaaagc aaaccagaca gacagattta gatttatttg tagagcaagc tggtttaaaa
    12301 gaaaagaaat taactcgtga aaatatacca ttatctgttg tagttccagc atttgctata
    12361 agtgaactaa aaactgcttt tcaaataggt tttttaatat atataccatt tttgataata
    12421 gaccttgttg tggcaagtgt tttgatgtct atgggtatgt ttatgttacc tccagttatg
    12481 atatcattac catttaaatt attattattt gtaatggtgg atggatggaa cttgatagta
    12541 aaaacattga tattgggatt tggataggag aaaaatagta tgagtgaatt tatgataatg
    12601 agtgttctta aaaatggcat gtttacagga gctatgattg cagctccagt gctaattgtt
    12661 tctcttgtag tggggctttt aataagtgta tttcaagcca caactcaaat ccaagagcaa
    12721 actttgactt ttgtaccaaa actaatagca attcttttag tagtagctat tggtggaagc
    12781 tggatgttac atactcttat tagctttact tcggagatat tcaatatgat gtctctcata
    12841 actaaataag taggatatat atgatttttg tatttattag aatagtagca ttctttggaa
    12901 cattaaggat actatttcca gctggtacac ctgctttatt taagtcgctc tttgcaatca
    12961 ttatatcaat cttgatttca agtactatgc aaattgaata tgcaacaaat atagacaaca
    13021 ttgtattgtt taccttatat ggtataaatg agaccataac tgggattatg cttggatata
    13081 taaccaatct atgtttttat agtatcagaa tggctggttc tatgatggac caacagatgg
    13141 gattatcaat gattaatatg tttgacccaa attcaatgac tcagacaact ttaatcaata
    13201 atcttatgaa ctggacagca cttatgatat tttttagcat ggatggtcat catgttttaa
    13261 ttagaggcat aaggtatagt tttgagttaa ttccaatagg gaaatcattc atagataata
    13321 atatagatta tataataaac atatttgtgc agtgcttttt aactggattc aaaatagcaa
    13381 tacctattgt actttgtctg cttatggcag attttatatt agggcttata tcaagaagtg
    13441 tacctcaact aaatgtaatg ataattggta tgccattaaa aatactggtt ggtattgcat
    13501 tatttatcat atcaatacct ttaattgtca atcaaataag tcatttatta agtcaaatac
    13561 caaagatgta tgagggaact tttgcactag ctcctatgtt ttttatgggt tcaacagata
    13621 aaacagaaga agctactcca aagaaaaagg gagaacaaag aaaaaaaggt aatatagcaa
    13681 agagtaaaga attacccatt gcaatgactc tacttgcatt cactttatta gtacctacat
    13741 tattttcata tgtagtgaat acacttaaat cttctcttaa ttattttttg agtctagatt
    13801 tttatatgaa cataaattat tcaagtatag agaaattagt tatagcagga ttaatggatt
    13861 tttttaagat atttttacca atagctattc cattcttagt tttaggtata gtcgcaaacc
    13921 tatttcaagt aggaatactg tttactggtg aaactttaaa acccaattta tcaaaattaa
    13981 atccaattag tggattcaaa aacatgtttt ctatgagaag tttgtcaacc ttaataaaag
    14041 atacagcgat tatatctatt ttggcttaca taggatatac attcttccaa gataattatt
    14101 tagatatatt aaagttaggt aacatatacc tgccaacttt aatgtacaca gttaaagatt
    14161 tagtatattc aatattaagt aagatttgtg tagctatgat agttatagca gttgcagatt
    14221 atgtttatca aagatacagt cataaaaagc aacttagaat gacaaaacaa gaagttaaag
    14281 atgaatataa aaactcagaa ggagacccag aaataaaagc taagataaaa caaaaacaaa
    14341 gacaaatttc gtctcaaaga accatgcaag cagtaccaag tgctactgta atagtaacca
    14401 atcccaccca cttatctata gcagtgagat atgaaaaagg gaaagaccaa gctccagtag
    14461 tggtggcaaa aggtgcagat tatttagctt ttaaaataag ggaaattgca aagggaaatg
    14521 acatacctat aatagaaaat aaacctattg ctaggttgtt atataagcaa gtagaaatag
    14581 accaagaaat tccagaggac atgtatcaag cttttgcaga gatattagta gcagtatata
    14641 aaatcaaaaa tagatacaaa gtccccaaaa ggtaggataa gtaatgaata agtttaattt
    14701 aaaggaaaat atggacgtaa tagtggcatt tggtgttgta gggattgttc ttatgatgat
    14761 agtgccgtta cctacattta tattagatat tttactggca attaacataa gtttatctgt
    14821 gctaatttta ctaatgacac tatttacaac aaatgtactt gatttatcta tatttccaac
    14881 agtgttactt gtgacaacac tatttagatt gggattaaat ctttcatcaa ctagattgat
    14941 tttaacacag ggatatgcag gtgaagttat agaggcattt ggaagttttg ttgtaggtgg
    15001 aaattacgta gtaggaataa ttatattctt aattatatta attgttcaat ttgttgttat
    15061 aaccaatggt tctggaagag tatctgaagt aacagctaga tttacgttag atgccatgcc
    15121 aggtaagcag atgagtatag atgcagattt aaatgcagga gttatagatg acaaaactgc
    15181 tagaaaaaga agaaaagagc ttcagcaaga agcagatttt tatggctcta tggatggagc
    15241 aaataagttt gtaaagggag atgccatagc aggtcttata gtaacagcaa taaacataat
    15301 aggtggaatt gtaataggag ctgtaatgct gaatatgact cttatgggtg cagcacagac
    15361 atatgtaaga cttactatag gggatggtct tgtaagccag atacctgctc tattaatatc
    15421 tacatcagca ggtataatcg taacaagagc ttctactgat gaaaactttg gtggattagt
    15481 aggtaaacag ttaacagcaa taccaaaagc agtaatgatg actggctgtg ttctaatcgt
    15541 acttggtatg atgcctggat tacctaaacc agcatttttt tcactaggta taggtgctat
    15601 agcagcaggt tactttttag gaagagaaaa gaaagatact ttagaagact tggagtttga
    15661 aaaacctgat atggtaaatc cagataaaaa tgaagtagaa gatgtaacaa acttaataaa
    15721 tgtagaatca atagaagtag aaattggata tggtttaata tctttagctg atgaatcagc
    15781 aggaggagat ttacttcaga gaatagcatc aataagaaga caatgtgcaa tagacatggg
    15841 tatagtcgta caacctataa gaataagaga taatttacag ttaaatccaa accaatacaa
    15901 tataaaaata aaagggaatg tagttggtgg gtatgagata atgcctaata tggttttatg
    15961 tatggatcct atgaatcaag gatttagtat atttggaata aagacaattg agcctacttt
    16021 tggaatagag gcactttgga tagaaagtga caaggtagaa gatgcagagc taaagggtta
    16081 tacagttgta gattcatcca caatacttat aactcactta ctagatataa taaaatcaaa
    16141 tgcacacgaa ttactgggaa gacaagaagt taagactatt atagatgcag caagagaaaa
    16201 ttatagtgct gtagtagatg aattgatacc agatttaatg acacttggtg aaattcaaaa
    16261 agtacttaaa aatctgttaa gagaaaaagt aaatataaag gatagagtta caatacttga
    16321 gacacttgca gaccagtcta gaaatacaaa agatatagag cttcttactg aatatgtaag
    16381 aatagctttg gctagaagta tttgtaataa tttagttgat gaagataagg caattgtagt
    16441 ggctacattg tctccagaaa ctgaagaatt ggtatctaat aatttacaaa ggtctgtaaa
    16501 tggaacttat ccagcagttg acccagaaaa tacaaataaa atatttgaga gtatacaaga
    16561 agtaatgaac aatgtatatt tcaatcataa tataccagta atagtagtat caccaaagat
    16621 aagagctcca tttagaaaat tagttgaaat tgtatatcct aatttgactg tattatcatt
    16681 aaatgaaata cctaacgata ttaaaatcaa agcagaaggt gtggtgagca tatatgatga
    16741 cgaaaaagta tacagctaac acaattcaag atgctatgaa tctagccaaa atggaattgg
    16801 gtgacaatat tacattaata gataaaaaag aggtaagaaa atctggaata agaggcattt
    16861 tttcaaagaa agatattgaa ttgactattg gatgggaaaa gaaagataat ttagaacaaa
    16921 aagatttaaa acgagagata gaacaactaa agtcaattat aaataatatg gggtttgata
    16981 ataaaaatga taatgatata gataaaatat gcaagaattt actaaactta gatttaaatc
    17041 aagaaattgt agaatttata aaagctgatt tacaagaaat gaagttcaat ggaattgata
    17101 caagtaaaaa tttagtagaa agtctaaaga aaaagattaa aatagaaaat caagcaataa
    17161 atggtaaaat tgctttagtt ggaccaccag gagtaggtaa aacgactaca atagcaaagt
    17221 tggctgccaa attagttttt gaggaaaata aaaaagtagg agtaataact atagatacat
    17281 acaggattgg tgctgtagaa cagttaaaga tatatactga tataatgaat attcctttta
    17341 aaggggtaat aagccctgat gagatggaat tggcacttga tgagatgaaa gattgtgatg
    17401 ttgtattaat agatacaaca ggtagaggct ataaaaactc tatgcaaata ttagaaatca
    17461 aaaatcttat agataaagct gaaactgata atatacattt agttttgaac tgtacaacaa
    17521 gagaaagtga tacaaaagca attatagatt catatagaaa tgtgaatttt aaaagcttaa
    17581 taattacaaa gttagatgaa acaataacat atggctcaat atttaacata atgaattatg
    17641 cacaaaaacc aatatcatat attacaacag gacaaaatgt tcctgatgat attataaaac
    17701 ctaatgaaga taagattata agattactat taggagttga aagtatatga tagaccaagc
    17761 tcaaattttg agaaaaatgg caatggaaaa aagaggttct gatgagatta tagtagagaa
    17821 tgagaatatg cctaagataa ttacaatagc ttctggtaaa ggcggcgttg gaaagagtaa
    17881 tctcgctaca aatttgtcta tatgcttaac caaacttgat aaaaaagttc ttatattaga
    17941 tgcagatata ggaatgtcta atatagatat aataatgggg gtaaatgtaa aagggactat
    18001 aattgatgta attaatggcg aaaaaaatat agaagatata atttcacaga ctaagtatgg
    18061 tgtaaatata atatctggag gttctgccct taatcatata gaagacttta cagaagctca
    18121 aagaaataag tttatacata gcatagaaca gattcacaat gtggatttta taataattga
    18181 tacaggagct ggtatgagca aaagcctatt gtcatttata tactgtagta ctgaattttt
    18241 cttaataaca actccagaac cgacatcatt gactgacgca tatagtttat taaaagctat
    18301 aagtaatttt ggcataaaga aaagtgcaaa tataataatc aatagggtaa ttgatttaga
    18361 agaagcgaaa tctacataca aaagaataaa aatggttgtg gacaaatttc taaaccttaa
    18421 tctggaattg tatggatata taatagatga caaaaaagta ggtgtatgtg taaaaaaaca
    18481 gataccattc attttagaat atcctacaag tattgcatca aaatgtatta tagctatagc
    18541 aaagagaatg gtaagtcagt caagagaaga tattgttgat aataaaagtt ttctaagaaa
    18601 gatgtttagt atatttaaaa gctaaggagg cgtagttaat gaatagagaa gaattaataa
    18661 aagagaatat gcctcttgta aagagtatag caagaaaatt ttttatacca gggaaaggtt
    18721 ttgaatatca agacttagta aatagtggag tcattggatt gattgatgca ataaataaat
    18781 ttgatactga aaaaggagca aagttttcta gttactctta tataaagata aaatctgcta
    18841 tattagatga aattagaaat caaagtccaa tttctaaaca taatctaact aaagtaaata
    18901 agtataatag agtggtagaa aagttgcaat ctaaattatt aagagagcca acttcatatg
    18961 aaattgcaaa agaattaaag gtttcagaaa aagaacttca tgatatcgaa agtaatatag
    19021 atatgttaaa tattgtatca ttaaactatg taatctttga agatacaaat gaaactgtac
    19081 aagatgtaat aagtgataga gaagaggaag ctccagaaaa tataatagaa gaagaagaaa
    19141 agttagagat tctatccaaa gcaattagta atttaaatga aagagaaaaa ttaatacttt
    19201 cactgtacta ttatgaagat ttaaatctaa aggagatagg gaaagtgcta ggtgtttcag
    19261 aatcaagagt ttcacaactc cacagaaaat caataagaaa cttaagaaac aagataaaag
    19321 aacttaaata ttctatatag atggtgattg ataagatgat acaaattata ttaatagtag
    19381 tgtctatttt aataatagta actatagtta taaatttaca gaaggaatca aaaaataaag
    19441 ataacagata ctttgataaa atatttgagc aagaatatga agcaaatgat agattaataa
    19501 ttgcagaaaa aagaagattc tttttagaca aagtacttgc agaaaaaaga attaatagtc
    19561 atataaaagt ttatgaaaat tataacctaa taaaggctaa tgaagaaaga aaggaaaaca
    19621 tatttataga atcaaaaaag gaagtaagac aagaaaatga gttatctctt aaagtgaaac
    19681 atcttataag ttcaggttat gatgatgtgg aaatatgtaa gatgctagat attggaaagg
    19741 gggaactgtt attaataagg agttgttaca aaatataagg atattattat gtgataaaaa
    19801 agtattctta ggaataggta ttggtatatt aatatcaact ttatgtataa tgctttttag
    19861 taataatgat atgagtaagg cagagataga aaaaaaggcc agagcgttag gcatgaaata
    19921 tacaagtgag atgaaagttt tagaatagat gatactgttt agacatttac aaaaaaggag
    19981 gtcataatgt gataagagga ttatatacag cagtttctgc tatgataact aatcaacaaa
    20041 agcaaaatgt tgttgtcaat aacctagcta atatggatac aaatggatat aaaagtaaac
    20101 aacttttgac taaaagtttt gatgaattaa agttggaggg ttacaataac tacgcaaatg
    20161 gaactaaaca aaagcagatt ataggcgata taagtccagg tatttctatg gatgaaacta
    20221 tcacaaattt taatcaagga cctataaaaa ctactgataa taaaatggat gtagctattc
    20281 aaggtaaggg attttttcta gttagtgatg cagctggaaa tcaattttac actagaaatg
    20341 ggaacttcag aacagacaat caaggtgacc ttataacaag tgaagggtat tatgtacttg
    20401 ggacgaatac tgctactggt gctgtaggtc atataaatgt aggaaatcaa aaatttgaag
    20461 tggcaaaaga taatacaata tcactagacg gaaatgctat gtataaattt aatattgtag
    20521 attttaataa ctacaataac ttaaaaaaag aaggagataa cttatattct ggaggaggag
    20581 ctatgaaagt aaataatgca cttacaatac aaagtgcgat agagtcctct aatgtagata
    20641 tggtatctga aattaataat atgatgacaa tatcaagaga atatgaggca aatcaaaaga
    20701 taattcaggc tatggattct aaattagcaa aaatagcaag tgagattggt tcagtaagat
    20761 aggtaatagg aggtgaattt ttatgtacac aatgttttat actagtaaaa cagctttatc
    20821 agcaaatcaa agtaaactag atataatatc taacaatata gcaaattctg attcaacagg
    20881 atataaaaaa ataaatatgg aatattctga tttagtggat gaagtactaa atagaccatc
    20941 ataccctaca aatggtaaag atatatcgac tggaacagga gctaaggcaa cagctcccat
    21001 gagaaattat gaacaaggtg cattggctcc tacagatagt aagagtaatt tggcgataga
    21061 tggagaagga ttctttagag tgataagaaa agatggtaca tatgcatata caagaaatgg
    21121 tggatttaat gtagatgctt taggtaagat tgtagatgat aatggaaata tattagatgt
    21181 ccaatttgat gcaggatata actataataa tacaaatata gatagtacaa acctgacagt
    21241 ttcaagaaat ggagagctat tcgtagataa taaaaaaata ggaaaaatta acttatatca
    21301 acctatagga actcaaaatt ttatatctga aggagatagt ttatttgtcg caactgatgc
    21361 agcacagatt aaacaagttg agaaagtcga tatagttcaa ggctatagag agaggtcaaa
    21421 tgtttcttta caagaagaat ttgttgactt aatagccact caaagagcat ttcaaatgaa
    21481 ctcaaaggga ataaagacag cagatgaaat gtggcaaata gcgaataatt taagagctaa
    21541 ataattatgt aaggagagga atattcatga atgatatgaa agagtacgat tttggcaaac
    21601 ctcagagtta ttcaagagaa aatttaactt gtttaaattt tttattagct gaattttgta
    21661 aaaaatataa aaactatata atttatgaac ttaagtgtaa ttctagtatg gttgttgata
    21721 gtatagacca agtaaattat cagacttttt tagatagagt aacttcttca tgcgtagtag
    21781 tacaaaatgc aatggaacct gttatgaaaa acttttcttt tagaatagat agaagtgtag
    21841 ctgatatgtg gattgatata acttctggag gaactggagt tgtaaaggat aatgatagag
    21901 agcttacaga acttgataaa aaagtgcttt tacaccttat ggatgattta gtaagaaata
    21961 tgcacttatt tgagggtttt gaaaatatac aagtattgga tacacataca catattaatt
    22021 tgccacaatt atgttctcca actgcaccag tatgcgttgt agatttaaaa gtgctaaatg
    22081 ataatgagtg tattggaagt gtttctcttt gttttccata caatggttta gaggctctgt
    22141 tagaaagtat atctgttata gaattttttg ataatgatgt aggagatgat tcagaggaat
    22201 ttacccaaaa aatctataat agtgtatcta atatagaatt atcagttatt gcagagatag
    22261 gaaaagtatc aattaatgtt gaggatttac ttaaactaga agttggagat gtaatagtaa
    22321 caaataaaaa aataaatgat tatatagata tatttgtaga aaattcaaaa tcatacacag
    22381 caacaccagg atttatatca agtaaaaaag gtgtaaaaat aaatgatgct gttggaaaag
    22441 aggtgtagtt atggacaata gtaatcttat ttcagatgaa gaagcaaaga tgttattaaa
    22501 tgataaagtt gaaagtgaag ttgatggaga tgatgagtta gccttagatg tctctgaagt
    22561 taatcgaaat aatatacaaa ggatacttga ccttaagtta gaattatctg tagttatagg
    22621 aagaactaag atgactctaa gagatatttt aaatttacat aaaggttctc ttattgaact
    22681 ggatacttta gcagaccaag atgtggaaat actaatagat aataaggttt tggcttatgg
    22741 aaaagtagtg gtggtagatt taaattttgg tgtaaagata acaagtatag taagtgaaga
    22801 agacatggta aaatctcttg tttagagata acatcaatta atacttaagg catgaggtgg
    22861 tacttatttt aaacttaaat gatataaaaa aagatatact aaaactcaat aaaaaagact
    22921 catgttctct atataatgaa tctattaata atataaataa taattgtata tcaactggat
    22981 taaaattttt aaaacaagca agagatttaa acccagatga tgttgatata ttaaatctca
    23041 caggtcttgt aaacttacta aaatgtaatt ttgatgatgc tgtagaaagt ttctataaga
    23101 gttcactttg tgaaaaaact gctctgtgtg aaaagtatat aaacatgctt acatctgagg
    23161 agtttttaat tttctttgaa aagtacaatc aacttataag gttaataaat aaaggaatgt
    23221 ataaagaagc tatacaaggg tttaaactca taactgacca atactgtgat ttaatagaac
    23281 cttatgagtt gcttgcctta ctgtatgaca aggaagaaga atatataaaa tttgatgaat
    23341 gtttagaagt attgaagatt attgataagg aaaattattt attaaatcaa gaaaataaca
    23401 cattagatat gtataatagt gctaatatac aaactaaaag taatataaaa gtagatgatt
    23461 ataagaatga gttcttacat aaaagtagta gtaaccataa aattaatgat tataatgatg
    23521 taactaaaga aaagagttca agcaaaaacc agaaaagttc agattctaat aagaaaaaaa
    23581 gtattaaaaa aaataagtac ctatatatag ccataatatt aggaatatta ttagtaggac
    23641 aaagtatata taattcttat aagatgaata atttaactga taaaatatct aaaaataatg
    23701 aaactaaaaa tgaagagtca atagattcta acttgactaa gaaagaaaat aatatagaag
    23761 aaaatgaaga tatgagtaag gcttctaata taaataagag taataaaaac attaataaag
    23821 aggatgatgt aaaatctaaa aaaaatactg tagaaaatga agatgataaa gattctgact
    23881 tatttacaga ggatgaattg atgtctaagg cgaatacttt aaaatcagaa aagaaaacta
    23941 aatcttctat acaactatat aagaaagttg cagatatagg tgaaaataaa gggaatacat
    24001 ctgaagcaac ttatcaagtt gctattctca gtgaaaagtt aaaagactat aaaactgctg
    24061 aagaatatta taaaatgtat gtagaaaatt actcagaaaa agatgcttat tttgatgaat
    24121 cttattataa tttaggtatg atgtactata acaatggtga tttaaaaaat tctaaattga
    24181 cattaaagaa gttggttaat aaggtaccaa atagtatgta taataattca aaagtaaaag
    24241 aaattttaaa agaagaatag ttaaattaga taagaaaaat aaattatttt attttaaaat
    24301 aatatgatta taatatattg acataccatt ttaaaaaatt taaaatatta attataaaat
    24361 gaggagggat aaaaaatgga atttgaaaaa gggagtattt caattcatac agaaaatatt
    24421 tttcctataa ttaaaaaatg gttatactct gataaagaca tatttataag agagcttata
    24481 agtaatggat gtgatgcagt aagtaaacat aaaagacttg tatcactagg agaaatatca
    24541 gaaaataagt catcagatta taaaattaca gtatctgtaa ataaaggaga aggtacactt
    24601 aaatttattg acaatggaat tggtatgact gaagaagaga taaaaaaata cataaatcaa
    24661 gttgcttttt caggtgctga agatttcttt aataaatata aggataaaat ggaagagtca
    24721 aatgatataa taggtcattt tggattagga ttttattctg catttatggt gtctaaaaag
    24781 gtacaaatag atacactttc atatacagaa ggagcgactc ctgtaagatg gatatctgaa
    24841 ggtggtacag aatacgaaat atctgaatca gatgctagaa atgatagagg aactactata
    24901 acactattta ttgatgatga tagtaaagaa tttttagatg aatttactgt aagaggaata
    24961 ataaataaat actgttcttt cttaccagta gaaatatatt tagaagatgt tgaaagatta
    25021 gaaagagaag ctaaagaagc agaagaaaaa gctaaaaaac aaaaggaaga tggaaaagaa
    25081 gagattgtag atgcaaaagt aattgagcct ttaaacgaca ctaatcctct atggttaaaa
    25141 tctccaaaag actgtactga tgaggagtat aaagagtttt atagaaaggt attcaatgta
    25201 tttgatgaac ctttattttg gatacattta aatgtagatt atcctttcaa tttaaagggt
    25261 atactttact tccctaaatt aaaaaatgag tttgaactga ctgaaggaaa agtaaaatta
    25321 tacaacaatc aagtgtttgt ggctgacaat ataaaagaag ttataccaga atttttactt
    25381 ttattaaaag gagttataga ctgcccagac ttaccattaa atgtatcaag aagtttctta
    25441 caaaatgaca gggatgtaag caaaatttct aaacatataa ttaaaaaagt agctgataag
    25501 ttaaaatctc tttgtaaaaa tgaaagagaa gaatacaata agttttggga tgatatacaa
    25561 atatttatta aatatggttg cttaaaagat gaaagtttct atgagaaggt aaaagaatgt
    25621 atattattca aaactattga tgatgagtat ataacattac aagattattt agaaaagtgt
    25681 aaggacaaac atgaaaataa ggtattttat gtaagtgata aagaacagca atctcaatac
    25741 attaaactat ttaaagaata cgacttaagt gctgttgttt taaactcatc aatagatact
    25801 cattttattt catttatgga atataaagag aatggagtta agtttaatag aatagatgca
    25861 gacctttctg atgtattaaa agataaaaat gaaaataaag atagtgaaga aaacaaagaa
    25921 gagatagcta agatagaagg tctgtttaaa gaagctgttg gagaaagagt gaagaattat
    25981 tctgtagaag gattgaaaaa tgaagacact ccagctatgg tgttagtatc agaacaatct
    26041 attagaatgg cagaaatgca gtcaagattt gctggaatgg atttaggaat gaattttgaa
    26101 gaagaaaaaa ctcttgtaat aaatgagaac agtccaataa tcaaaaaatt ggtttctctt
    26161 aaagatgatg aggaaaagaa agataagatt acgcttattt gtaaccagat agcagattta
    26221 gctttacttt ctaataaaga attaaagcca gatgagttag attcatttgt acaaagaagt
    26281 aataagctta tgagtctttt gatagaatta taagattgaa tttgaataca ttgaagcaaa
    26341 tttataaaaa gtattcaata tcagtgataa gaaagaagtg tctcaaaatg aacttttcag
    26401 ttcatgaggc gcttttttta tgtgaaatta aatttatttt aatcatttca acaatgaaaa
    26461 atatatttat ctctattttg agacagaccg ttctttattt ttttatcttt ggttaatttt
    26521 taaatattta agtaggttct aataaaaaac tctatttagt taaatatagt taacttgata
    26581 tataataaga taatacatat aattaagtaa aagattgttt tttagtttat gtttaaatac
    26641 ataattacac tttgttaaaa acataaaatt attactaaag tagaaatagg aggttttagc
    26701 atgaatttta attataattt gccagtaaat atcttatttg ggagcggcag aattaatgat
    26761 gtgggaaaag aagtatctaa atatggcaaa aaagtattga tagtaacagg taaaaatagt
    26821 acaaagaaaa ctggattact tgataaaaca atagatttac taaaagactc taaaatagaa
    26881 tatgaagttt ttgatagagt agaacaaaat cctcttataa ctactatata ttcaggagta
    26941 gaaattataa aatcaactgg atgtgattgt gttttaggtc ttggtggtgg aagcattatg
    27001 gatgctgcca agagtatagc tttttcttat aaaaacccag gagatttaaa tgattatata
    27061 tttggaatta gacaaggtga agaagcttta cctataatct tagttccaac aacatcaggt
    27121 acaggtagtg aaggaaattg ttttgcagta cttacaaatt cagaaactaa agataaaaaa
    27181 tctataaaga agaatagtat gtatgcaaaa gcatcaataa ttgacccaga acttatggtt
    27241 acaatgccaa aacatataat tgcatcagtt ggatttgatg cacttgccca taatatggaa
    27301 gcatatcttt ctaatggaag aaaccctctt gcagatgtac aagctattta tgggattgaa
    27361 cttatatcag aaaacctaat aaaggtatat aatgatgtta atgatttaga agcatgggaa
    27421 aaaataacac ttgcaagtac tattggagga atgacgattg gtacagcggg tacagccttg
    27481 ccacatggaa tggagcatcc tgtaagtggt cttagagatg taatgcatgg aaaaggactt
    27541 gcagctttga ctcctactat tatagaaagt tcatgggata gtgatattga aagatttggt
    27601 aatatttcaa aggtattagg aggaacttgt gcagaagatt gttcagatgc aattagaaat
    27661 ttccttaaaa aaatagattt agatataaag ctaagtgatt taggtattga aaaatcagat
    27721 atagattggt taactgataa ttgtatgaaa atatcagttg caaatattaa gagacatcca
    27781 aagtatttta ataaggaaca aataaaggaa atgtaccata agtctttata atttgacttt
    27841 acatatttag ttttatataa aaattataaa ttaataataa aaaattataa gtaattaata
    27901 gtaaaaaaat tgtgtataga agttatatat ataataaatt tgtcttaagc ttttatttta
    27961 ctttctaata aataaaggag tttaaaaaag ggaagattaa ttaagtaata ttttataaaa
    28021 ttcttcgatt taaaaacttt aagtagaggt gaaataaatg tttaatccca aaagaataat
    28081 attcgaaaaa ggaactttag acactgatgt gggtaaaaaa atatacaata aatttagaga
    28141 taataaagat gtagagataa ttaatatatc atcaaatagg attaaagaac atattccagg
    28201 tgaaaatcta tatgaacaat atagaagtgg gaagcagact atggtagttg ggattagaaa
    28261 aagtctgaag tttcaaacat gtaaaccttc tgctaattat caactaccac ttgtcagtgg
    28321 atgtatggga agatgtgagt attgttattt gaacacacaa ttaggtgata aaccatttat
    28381 aagggtgttt gtaaatgtag atgaaatact tgaacagaca aaaaaatata ttgaagatag
    28441 aaagccacag gtaaccatat ttgaaggggc agctacatca gacccagttc cagtagagcc
    28501 atatactaat tctttgaaga aaacaataga gttttttgga agagaggaaa aaggaagatt
    28561 tagatttgtt actaaataca gtgatgtgga tagcttattg gatgctgaac ataataatca
    28621 tacagaaata cgatttagta tcaatactcc tagaattata aatgaacatg agcattatac
    28681 agcctctgca aataaaagaa tagagtcagc tgtaaaactt gctagggctg gttatcagat
    28741 tggtttcata atagcacctg tgtttttata tgaaggttgg aaagaagagt ataaaaatct
    28801 gattataagt ataaaagaag ctttaccaaa ggattttgat aaaaaaatta tatttgaagt
    28861 tatatctcat agatatacaa ctaaagcaaa aaatagaata ctagagattt atccagatac
    28921 actattgcca atggaaaatg aagatagaaa gtttaaatac ggtcaatttg gatatggtaa
    28981 gtacgtgtat gataaagaac aattagatga tatgaaagag ttctttctta aggaaataaa
    29041 tggagtattt aaaaatagtt taataaaata tataatatag caaatcttac ctaaagattt
    29101 gctatatttt atttttatat attttatgtt tataataaag caatgagatt tagcattatc
    29161 atataagaaa attaaaaagg gcactatcta tcaagaacat tttttctagt tttgatacta
    29221 taaaggataa gagtaacaaa aatatcagga ggagttatag tatgcatgca tgggagtcta
    29281 ttcaaattac attggattta atagaagcaa atttatcaga agaaatatca attaatgaat
    29341 tagcaaataa agcaaattta tcaccatttt actatcaaag actatttaaa agattggtaa
    29401 ataagactgt aatggagtat ataaagcttc gtcgtcttgc aagagcttct gaatacttag
    29461 cagaacataa gaatcgtata cttgatgtag ctttaaattt tggatttgct agtcatgaga
    29521 catttacacg ttcctttaaa aaagcatatg gattaactcc agaaaaatat agagcaaatc
    29581 cagtaaaact caatcatttt attaagccag aattaatttt aaattatgct atggttgatg
    29641 aaggtgttcc actgattgca gatgatatag tgattgaagt caatcgtaag acattgagta
    29701 atccaaggac atttgttgga atagaaatag aagtacctat ttgccagttg gtgggcggtg
    29761 aaacaactgg aattgcaata gtagaggaat tatggatgaa acttggaagt caaagacata
    29821 atataccaaa tcaaattcca ggtggaaatg aatttgctgc tctttatatg ggaaatgcaa
    29881 aagaaggaaa ttgtatgtat atggcaggtg tagaggtaga gtcaggtaca tcagtagaag
    29941 gatatagtac ttttgagttg ccagctaaag aatatttagt atgtggattt gaggcagaaa
    30001 actttaatga gttagttaat tcagctgttt ttaaagcaga taaatttatg gaaagatgga
    30061 tgaagaaaca taatttaaca actactgatt ttgcaattga gatgtattac cccactacac
    30121 ctgaagcagc atatctggaa cattggattg ttccggttcc tattgaacaa tagaaaggat
    30181 gatgaatatg aattggcaag atgcgttttt tcaagatact aaacctacat ttgagcaaat
    30241 taatgaattt attaataatc cattatggga caagttaaat aaaactttgg tttctactta
    30301 taatgtagaa cctaagcttg aatacagtaa atgttcaatg caaagaggtt ggaatgtaaa
    30361 atataaaaaa agaggtaaat cactatgtac tttatatcca caagaaggtt attttaaagc
    30421 tttggttatt gtaagtgaaa gcaatagagt agaggttgat ttatttatta atacttgttg
    30481 tgattatatc aaaaaaatat ataatgaggt aaactttttt aatggaagta aatggctaat
    30541 gattcaagta gatagtttat tggtattaaa cgatatgctt gaactaatta aatttagagc
    30601 ttaacatata gaacaggatg aatatataag tcatcctgtt tttatattca atcagataaa
    30661 ttaatatata gtctataata tgaataattt agtaaattta aatttacttt atctttctac
    30721 attctttgca actaagtcca catgtgtttt tttcttgcat attagtagca ccccaattag
    30781 caaattgttg taaaataggc acaatacttt tacctttact tgttaatgta tattcaactt
    30841 ttggaggaat ttcattatat tgtttacgat gaacaatacc agtagactct aaatctttta
    30901 atgattgagc tagcatagta tttgtaatgc catccaattt tctttttatg ctactatatc
    30961 taattggatt ttcaactgct aacatacata taataggtaa tttccactta ccaccgacaa
    31021 tgtctaatgc ataacggatt ggacaggtta attcttcaac gtttttagtt atattttttt
    31081 cagtatttat actcatttta attactcctt agttatagta gtatcttttt tatactttat
    31141 cagaaaaaac tgcatacttg ttttttctgt attttatatt aaaattatat caaaggataa
    31201 tccttaatac aagtatttaa aaggggtgta agaattatga aagcattaga atttttgaac
    31261 aaggcaggag tttattattt agcaacagtt gatgagaaca atcaagcaca tgtgcgtcct
    31321 ttaggatttg ttatggaata caatggtaaa cttacatttt gcacatctaa tcaaaaggat
    31381 atgtttaagc aacttatttt taatccaaag gttgaacttt gttgtataga ccaaaacctt
    31441 aatactctac gtattttagg tgaagcagta tttgttacat caaaagaaac acaagctaag
    31501 gcacttgaaa taatgccaag tttaggtcag atgtactctg taggagatgg taagtttgaa
    31561 atattctcta ttgataaggc aaaagttagt tgttgttcaa tgagtggtaa aaagattgat
    31621 gtagagcttt aattataatt gaatcgacat ccatagcgaa aattaagaaa acgttattac
    31681 tgaaatattg atagatttaa tttaaatgca ataaaaagat ttttaactaa tgaatatgta
    31741 aaaattttta gttattagat gtgtatttca ataatgatat actattttga ataattacat
    31801 actagaaata agat
//
LOCUS       DAELTA010000029        30715 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.029, whole genome shotgun sequence.
ACCESSION   DAELTA010000029 DAELTA010000000
VERSION     DAELTA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.029"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..2001
                     /locus_tag="KPG13_001154"
     CDS             100..2001
                     /locus_tag="KPG13_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0984892.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2004..3047
                     /gene="ypdE"
                     /locus_tag="KPG13_001155"
     CDS             2004..3047
                     /gene="ypdE"
                     /locus_tag="KPG13_001155"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG0984893.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3062..3367
                     /locus_tag="KPG13_001156"
     CDS             3062..3367
                     /locus_tag="KPG13_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0984894.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3464..3799
                     /locus_tag="KPG13_001157"
     CDS             3464..3799
                     /locus_tag="KPG13_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0984895.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3831..5153
                     /locus_tag="KPG13_001158"
     CDS             3831..5153
                     /locus_tag="KPG13_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0984896.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            5163..6233
                     /locus_tag="KPG13_001159"
     CDS             5163..6233
                     /locus_tag="KPG13_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0984897.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6238..7425
                     /locus_tag="KPG13_001160"
     CDS             6238..7425
                     /locus_tag="KPG13_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0984898.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7622..8494)
                     /locus_tag="KPG13_001161"
     CDS             complement(7622..8494)
                     /locus_tag="KPG13_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG0984899.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8888..9079)
                     /locus_tag="KPG13_001162"
     CDS             complement(8888..9079)
                     /locus_tag="KPG13_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984900.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9299..10345
                     /gene="cobT"
                     /locus_tag="KPG13_001163"
     CDS             9299..10345
                     /gene="cobT"
                     /locus_tag="KPG13_001163"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG0984901.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10377..10937
                     /gene="cobU"
                     /locus_tag="KPG13_001164"
     CDS             10377..10937
                     /gene="cobU"
                     /locus_tag="KPG13_001164"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG0984902.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10947..11720
                     /gene="cobS"
                     /locus_tag="KPG13_001165"
     CDS             10947..11720
                     /gene="cobS"
                     /locus_tag="KPG13_001165"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG0984903.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11740..12354
                     /locus_tag="KPG13_001166"
     CDS             11740..12354
                     /locus_tag="KPG13_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0984904.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12421..12600
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12730..14229
                     /locus_tag="KPG13_001167"
     CDS             12730..14229
                     /locus_tag="KPG13_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG0984905.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14268..15644
                     /locus_tag="KPG13_001168"
     CDS             14268..15644
                     /locus_tag="KPG13_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG0984906.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15645..16616
                     /locus_tag="KPG13_001169"
     CDS             15645..16616
                     /locus_tag="KPG13_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0984907.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16648..17718
                     /locus_tag="KPG13_001170"
     CDS             16648..17718
                     /locus_tag="KPG13_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0984908.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17720..18607
                     /locus_tag="KPG13_001171"
     CDS             17720..18607
                     /locus_tag="KPG13_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG0984909.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18595..19227
                     /locus_tag="KPG13_001172"
     CDS             18595..19227
                     /locus_tag="KPG13_001172"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG0984910.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19236..20462
                     /locus_tag="KPG13_001173"
     CDS             19236..20462
                     /locus_tag="KPG13_001173"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG0984911.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20459..21067
                     /locus_tag="KPG13_001174"
     CDS             20459..21067
                     /locus_tag="KPG13_001174"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG0984912.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21057..21632
                     /locus_tag="KPG13_001175"
     CDS             21057..21632
                     /locus_tag="KPG13_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG0984913.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21625..22377
                     /locus_tag="KPG13_001176"
     CDS             21625..22377
                     /locus_tag="KPG13_001176"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG0984914.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22361..23482
                     /gene="cbiG"
                     /locus_tag="KPG13_001177"
     CDS             22361..23482
                     /gene="cbiG"
                     /locus_tag="KPG13_001177"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG0984915.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23479..24204
                     /gene="cobJ"
                     /locus_tag="KPG13_001178"
     CDS             23479..24204
                     /gene="cobJ"
                     /locus_tag="KPG13_001178"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG0984916.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24201..24953
                     /gene="cobK"
                     /locus_tag="KPG13_001179"
     CDS             24201..24953
                     /gene="cobK"
                     /locus_tag="KPG13_001179"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG0984917.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24972..26450
                     /locus_tag="KPG13_001180"
     CDS             24972..26450
                     /locus_tag="KPG13_001180"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG0984918.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26480..27385
                     /gene="hemC"
                     /locus_tag="KPG13_001181"
     CDS             26480..27385
                     /gene="hemC"
                     /locus_tag="KPG13_001181"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG0984919.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27375..28874
                     /gene="cobA"
                     /locus_tag="KPG13_001182"
     CDS             27375..28874
                     /gene="cobA"
                     /locus_tag="KPG13_001182"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG0984920.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28891..29856
                     /gene="hemB"
                     /locus_tag="KPG13_001183"
     CDS             28891..29856
                     /gene="hemB"
                     /locus_tag="KPG13_001183"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG0984921.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30009..30587
                     /locus_tag="KPG13_001184"
     CDS             30009..30587
                     /locus_tag="KPG13_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG0984922.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12439 a         3068 c         5678 g         9530 t
ORIGIN      
        1 atataaaatt cttaaataaa aagaaaaggt tgtttttata aaaaatattt tttataccac
       61 tttaaaaaac acaataataa cactgaggga ggtggggata tgagagaacg cattattctt
      121 gaaacattaa aaaatagtag tggttatcta aacatagatt attttgcaga taagttgggg
      181 gtatctactc gaactatcag aaatgaaata aaaaagatag aaactataaa agaatgtaat
      241 ggttttaaat tagaatataa gtctaaatta ggatatattc taaatattaa agaccaagat
      301 aaattcgagc attatctaag aaacttaccc tcagatttag ttgaaaatcc agaacaacgg
      361 ttggagtcta taatagtaga gcttttagta aatgaaggtt ataaaactat agaacagtta
      421 agtaaaaaat ttctagtaag ttcatcccaa ataaaaaatg atttaaaaaa gatagatgaa
      481 aagataaagg atacagaatt aaaactagag cgaaaggctc attatggaat taaaatagaa
      541 ggttctataa aagaaattca aagtatattg gtggatagtt attttagagg gaatagaaat
      601 ataacagagt acaggaataa gtttatagat aatttaaaat tagctaatat tagaagtaca
      661 ataaaaaatg tacttaatga acatgattta gaagcaaatt taacagaact tgaggaaata
      721 ttagcacaaa ttattatatt gtatataaga gttagtatga gagtagtggg aaatgctagc
      781 gatttaaaat tatatacaga agatttaatt atagataaat tattagataa aatttttaga
      841 gataaaaaat ataaccttaa ttatgatgaa aagtattatt taaaggagct tataaaacta
      901 aagactaaag ataagaaagc cactataaag aatattgata agaataaact tcaagatatt
      961 atgttcgaat tttttagaga gatagataaa aaatataact ctaatttttt agaagataaa
     1021 gagtttttta acttatttta cttacatatt gcatgtttaa tagaacgtat aaagaagaac
     1081 cacaagataa taaatccttt ttcagttaaa ataagtcaac agtatccaac tgtttttaat
     1141 ttatctattc aattttcaaa aatcatagaa aatgagtatc atataaaaat aagtcaagat
     1201 gaaatagggt ttatagctac tcacattgct gttccttttg aaaagagaga agaagctaat
     1261 tttaataaaa aatataaaat agcaataata tgctcttctg gtggaggtag tgcatttttg
     1321 ataaatttaa gacttaaaga aatttttcca aatgctgaga taagaaattt ttcattacta
     1381 gaagaaaaag ctgtaataga atttgcacca gatttgattt tttcaataac aaatctttta
     1441 tttgaaataa atgctccagt tatacttata aatgaaattt tagacgaatt ggattattta
     1501 gatattaaag aaagtgttag atttgcagac catataggaa atatatcaag cccaaaacaa
     1561 tatatattag gtctatttga taagaatcac tttaggtgta taaaagataa agctaagtat
     1621 aaggatatcc ttgatagtat gtcaaaatgt atagttgacg agggagcttg ttctccaacc
     1681 tatcccaaag atgtatggga aagagaaagt tatttgagta ctatttatac aaatggggtg
     1741 gctatacctc atccaatcga gatgacagga aacaaaaata taatatcagt agctttgata
     1801 caaactgata ttatttatga aaatagggtg cctaaaatta ttttcatgat ttcattaata
     1861 aagggaaatt tagaacttca taagcaaata tctaaatatt taagtaagat tatggcaaat
     1921 aaggatatgg tagacatgct aaataagtca caatcttatg aagagtttat gtataagtta
     1981 aaaatctatc tgggaggtta gacatgaata tagagttttt taagaggttg tcagaagcag
     2041 atggtatagc atctaatgaa gaagaggtta gacatgtttt attggaagaa ctaaaagctt
     2101 atagtgataa aattatctgt gatgggctag ggagtatcat cttttctaaa ataaaagatg
     2161 agagtgcacc aaacgtaatg atttgtgctc atatggatga agttggattt atggtgagaa
     2221 gtatagacaa acttggtatg attcacttaa taacaatagg aggagtaaaa cctcttgcac
     2281 agtttgtaca aaaagttaga attacaacaa aagaagggaa gaaaatcccc gctgtcataa
     2341 atgctacata taataatggt aaggctgaaa atatatatgc agatattgga gcatatacag
     2401 aagaagatgt atataaccta gggattaatg ttggggatat ggtaacatat acaacaagct
     2461 ttgaggaatt tactttacca gaccgattag tgggaaaggc ttttgatgat aggataggat
     2521 gttttgtgat gggagaagtt ttaaaagaac ttaggaaaga aaatctaaat tgtaatattc
     2581 actttgcggc tacaagtagt gaagaggttg gaataagggg agcaaagaca tcaacacagt
     2641 taataaatcc agatatagta tttgtaatag atgtagcatg tgctaaaaat gagtttgtga
     2701 gagactatac aaataagaaa caaattgata aaggtgtaat gctaatgcat agagatagga
     2761 ctctagtccc tagtagaaaa atgatagatt atgtaatgga aattgctgat aaaaataata
     2821 taccacttca acatgatatg tttgaatcag gtgggactga tggaggagaa gcacatctcg
     2881 taaatgaagg gaaaccttgt gtagtaactt gtgtaccagt tagatatggt cattgtgcat
     2941 tttcaatagt aagtaataaa gatttggaga acattataag attgtataca cagttaattt
     3001 taaattttga tgaagaacaa tataaacata taagaaattt tatctaaagg agaataagaa
     3061 aatgtcaaca gaagtacaaa taatggaact tatatcaact gcaggagaga gtaaatcgaa
     3121 agcttttgag gctttaaaaa aagtgaagtc aaaagatttt aaaggagcaa aattgttaat
     3181 agaggaggct cgtaaaatag atattgaagc tcacaggata caaacagaat taataactca
     3241 agatttaaat gaagatgttg aaagtccagc aatgagtctt ttaatggtac atgctcaaga
     3301 ccactatatg acttctcagc tagcaaaaga cttgatagag gctttgatat attgtcttga
     3361 taagtaattg taatatagaa atagattatt tatgtaaaaa ttcttttaaa agtttagatg
     3421 cttttaagag ataataaaaa tacaaacttt aggaggaatt attatgaaaa tattattatg
     3481 ttgtgcaggt ggattttcta ctaatatgtt aatgcaaaat atgaaaaagg tggtaaaaga
     3541 gagtgctaaa ctcaatgaaa aagattttga gtttacagct ataccagcag attcaataga
     3601 aagtgaagtt gatgattggg atatagtact tataggtcct caaatagctc acaaaataga
     3661 cttcttaaaa ggaatattag agccaaaaaa taagcctttt gcagtaatag ataaagatgt
     3721 atatggaaaa atggatggag ctacagttat gaagttggca ttaataactt ataagaaaca
     3781 tcaattagct caaggataat aaaaataatg agggaaaagg gagattagat atgttttcaa
     3841 aatttgaagc atttatgaat agagtattta tgcctttagc tcataaggtt gacaatcaac
     3901 gtcaccttgg ttctataaag gctggtatgg ttgctatgac accattcaca atattaggaa
     3961 gtatatttgc aatacttcca gcacttccaa atatgcttgg agaaaataat ccaataagca
     4021 aattcatact tagcaattca gcaatattgg atttaccagt aagtctttcc ataggtttaa
     4081 taggattata tgcttgtatg tgtattgctt ttaatctggg gaactactat aaattatata
     4141 taccaggttg tgttgcacta tcaacttttt cttttttatt cctagtagcc tcatttacac
     4201 cagatggagc tttagaaata ggaaatttag gagctaaagg gctatttaca ggtatgatag
     4261 cagggcttgg ttctgtggag ttatataatt tttgtaataa gaagaatatc acaataaaaa
     4321 tgccagaaaa tgtacctgat tttgtatcta ggtcttttga attaatacct actactataa
     4381 tagtttgtgg aacttttata gttgctcgtt ttatttcatt aaaagtattt ggagaattac
     4441 ctccacagat acttactcaa tttttatctc cattagtagg aagtatggat aacccatggt
     4501 cagttcttgc acttaacttt gctatatgta ctatattctt ttttggaata cattcatcag
     4561 tatttagtcc tataactaga cctataatga ttacatttat agctgaaaac atagcagcta
     4621 tgcaggctgg agaaacaata cctcatttct ttacagcagg agcttcaagt gcattctttg
     4681 gatttacagg atgtggaatt agtataggtt gtgtaattgc atgtatgtta tcaaaaaatg
     4741 cttcctataa gaagattgga cgtgtatcat tattccctgc attatttggg ataaatgaac
     4801 caatattatt tggagcaccg ataatattaa atccaataat gtttatacca catgtatttg
     4861 gtggagctat aataggtaca ctaccaatgt tttttatgca ctggggatgg ttagcaaagc
     4921 cagtatttga ccctccatat gtaggggtat tcttagaagg atttttaact aatggagatt
     4981 ggcattctat attagcaaat gcattgcaat tagtattagc agttttatta tattggccat
     5041 tctttaaaat aatggaacgt caacaaaata ataaaaaaca aaaagaagaa actaagaata
     5101 tattctctaa agaagaagaa ggcttattgg atgatttagg attggatttc taatattttg
     5161 ttatgaataa tacaatggag agattagtat ccttaatcta taaaagaggt gttgacgctc
     5221 ttttgattaa agcaaaaaat actaaaagat atataggagc tttatcaggt agtggtgtat
     5281 atgtgcttgt tactaaggat aaaaattatc aaattctaga tggcagatat attgatgaag
     5341 cagataaaaa aacgactggt ttcataaaaa ggattgtatc tcaaggttct tatataccaa
     5401 ctataattga gttattaaaa gaattaaata taaaaaagtt agcaatagaa aaccaagcta
     5461 tgagtataca agagtatctt tcactaaaaa atgaaggttt tgaaatcaat ttaataacaa
     5521 atgaattatg gaagctaagg gctataaaat ctaaagaaga aattaaactt attaaaaagg
     5581 cttgtgagat tacagatgaa gtatttgatg aggttatttc tgaaattaaa aaagatatga
     5641 ctgagctaga agtatcagct ctaattcagt atcatgcatt aaaaaaaggt gcaagtggaa
     5701 tgtcttttga aacaatagtg gtatcaggag aaagaggtgc aatgccacat ggaagaccta
     5761 caaacaaaaa attgaaagtg aatgaagcta taacaataga ttttggtgtt gtttatcaag
     5821 ggtatcaatc agacatgact cgtacaatat ctataggcaa acctcctaaa attattaaag
     5881 agatatatga tgtagtattg gaagcacaat tatctgcaat agagtcaata aaggaaggaa
     5941 ctagggcaag tgatgttgac aaagtggcta gaaaaattat agataaacat ggatttggcg
     6001 aatattttaa tcatggactg ggtcatggta taggtcttgg agatggtgaa gttcccactt
     6061 taaatccaaa tagtgaagat atattagtgg aaggaatggt aatgtcttgt gagccaggta
     6121 tttatattcc aaatgtaggg ggagttagaa tagaagatga tattgttata atagatggaa
     6181 aaggtgttcc actcaataag acatccaaag agtttataat attaggagag taaaattatg
     6241 aaatataatt ttaataaagt attaaatcgt aaagaaggaa attgtagaaa atggagcaac
     6301 catgtgatta aagaaaaatt tggcttaaat gaagatgcaa ttcctatgga tttagctgat
     6361 atcgattttg agtgtgctcc tgctataaaa gaatctatct taaaaagagc atcagtagga
     6421 gattatagtt atacttttat aggagatgac ttctatgatg ctattataaa ttggaataaa
     6481 agaagatttg atgtatatat agaaaaggat tggataaaat taacttttgg tactgtttca
     6541 acccttcatt atatagtcca agcatatact aaagaaggag agggtgtatt gataaataca
     6601 cctgcttatg acccttttgc agaggctgta gtaaataata atagaaagtt gtgttgtagc
     6661 tcattaaaat tagataacaa tagatattat ttagatttag aggatatgga aaatcaaatg
     6721 aaatacgaga atataagatt gcttatattc tgtagtccac aaaatccatc aggtcgtata
     6781 tggacaaaag aagaactata tcaagtgtca gagttatgtt taaagtataa tgtaattttg
     6841 gtaagtgatg aaattcatag agatataata tttaaagatt atagatttgt atctttgtgg
     6901 aatgctcatc cagaaattta taaaaatagt ataatctgtg tatctcctaa taaaggcttc
     6961 aacttaggtg gattaaaaac atcatatgta ttaataagag atgtaaaaat tagggagact
     7021 ttattagaaa ggctaaaaag taattcaata acttctccaa atgtatttgc aataccagca
     7081 atagttgcag catataatga aagcgaagaa tggctagatg ctatgactag ttatgtagag
     7141 gagaattttg aaatagttta tgatttcttt gaaactaata taccaaaagc taaagtgatg
     7201 aagtcagact cttctttttt agcctggata gatgtaagag aagtatttag aaatgaagaa
     7261 gaatctaaag aattttttag acatgcaaac ttaactatgg tagttggaag ttattttgtg
     7321 aaagatggag atggatttat tcgtattaat gtggggtgtc ctagagaaac attaaatgag
     7381 gctctaaata gaatcaaaaa aacgtatata agtatgtatt gttaaatcat aaaaaaagta
     7441 tctatggttt atataaaaac acattattta gttatcaaat attactggaa ttttgtattc
     7501 cagtaatatt ttttgattaa ggaatattat tgaggaatat taaataatat tatttcatac
     7561 ccttatatta acttatataa aaatatttta ttattttggg aacgggttag ctaaccttta
     7621 tctagtgtat atttatattg taagaagcag taaaaacttt tcctttacta agtttattaa
     7681 taaacttctt atctttataa attttgttag aatttatact atcagcaaga ccataccaag
     7741 gttctataca taaaaaagga gcagtagaat tagtttcact ataataagga gtccaaatac
     7801 caactagagg gaaatcttcc atagatatag ttacatattt actaccatca ttattacata
     7861 tagacacttc atctatgttg gtgtatatca aagcatcatt cttgaatact tctggattta
     7921 actgtaagtt ttttaatttt ccaataggta ttatatcatc tgtaaaagaa ccatttaaat
     7981 tgattttttc tacatcattt ttacttttaa actcaaggta gtgtttagaa aaatcatttt
     8041 gttcgtaaaa aggaaggttg aatgcaggat gtccacctat agaaaagaat atatctttag
     8101 aatcagtatt ttttacaatc cattctacct caatactatt atcatttaat gtatagttta
     8161 tgattagctc aaaagaatat ggatacttct ttaaagtaga ttcattatct gttaatttat
     8221 aagttacaaa tgtatctcct ttatctacaa tctcaaagtc catatctcta gcaaacccat
     8281 gttgagtcat gctatataaa ttttcttcta ttattgtttc attatcaaaa agtttaccta
     8341 ctatagggaa caatataggt gattgtctac cccaatattt tttattacca tcccaaagaa
     8401 tgtttttgtc gtatttttta gaaaatattt tagttagttc agcaccaaaa ctgcttgatt
     8461 cgataattaa attttcattt tgtaatatat tcatttaaaa tctcctttat tatattttat
     8521 gagtatatta tactataaag tataaagatt attaaatttt atttgatatt atatttgaaa
     8581 ggtagatttt agtatatttt gaaaattttg ttttgaaaat ttgggattat ttaagaaatt
     8641 ttgtattata aatagaagga aatagataaa ttgtatagaa taaaatatat taagataagt
     8701 taataatgtt aaatgggaag tgagtttatg gaaaaaccaa atacaaaagt tgttaacttt
     8761 caagagtata aagacagaaa aaaccaaaga gaagtaaata gagacgaact tttaaagctt
     8821 ataaaaaaaa ttgtgacttc aaaataggga gttgttaaac tccctatttt aatgggtttc
     8881 ataataatta agacattcta agtccatcta tataatcttt gaattgtgag aaatctgatt
     8941 cactacttaa ttctatggtt tctgaacctt ccgtataaaa attcttaaga tagatagtat
     9001 aattattatc aacacaatca tgataaatac atctatatcc atcttcatac aaacttttta
     9061 acttagaaaa atcatccaca atatcatctc cttaaagttc ttttaaatat atttttacaa
     9121 aaagcatcta attgtatgca aattgacaat gtaaaataaa ggaaatgaac ataaaatgta
     9181 gaataatttt tatatgtatg taaaaataaa aagaaaaagt tgaaattttt agaaaataag
     9241 agtatttttt atctaaaaag agagtttaaa aaataaaaaa atgttgggag gataaaaaat
     9301 gagtttatta gaaagtatat caaaaaacat atattcccta gataactctt ctattgaaaa
     9361 aacaaagcag agattggata ggcttataca tccaactgga agcttaggaa agatagaaga
     9421 tatttgcatg caattagcag gaatatttgg caatgaaaat tttgacacaa gtaaaaaggt
     9481 tataattgct tttgcgggag accatggtgt atatgaagaa ggcgtagcac ctgaccctca
     9541 aaatataaca aaattacagt ttccaaattt ttcgaaaggt ctatgcggag ttggagtaat
     9601 aagtaaattt gtaggggcag atgttgtagc tgtagatgta ggaataaact gtgatgaaaa
     9661 actggatggg gtattagact acaagataag aaaagggaca tcaaatatgg caaaaggtcc
     9721 agctatgagt aaacaagaag caattagatg tttagagata ggtattgaaa tagcagagca
     9781 gtgtatagaa aaagattata aggttattgg tattggggag atgggtatag caaatactac
     9841 tccaagtaca gcaataatat ctgtaatttc agggtgtgac ccgttagaag ttactggtat
     9901 aggtgcaggt cttaagaaag aaagactaaa gcataaggct gaagtaatta gaaaggctat
     9961 tgaaattaat aatccaaatc caactgatgg agtagatatt ttatctaaag taggagggtt
    10021 tgaaataggc tcaatggcag gtgttatatt aggttgtagt gctaatagga ttccagtagt
    10081 aattgatggg tttatttctt atgcagcagc attaattgca tataagataa atccaaaaac
    10141 tagagaatat atgatagcat ctcatttatc agcagaatca ggaactaaaa gagcattaga
    10201 catattaaaa ttagaccctc tattaaatat ggatatgaga ttaggtgagg gaagtggagc
    10261 tgctttagca tttgatataa tagaagcttc aaactatact tataaaaata tggcaacttt
    10321 tgatgaaatt gacatgggaa gataaatata aaaatttaat atgtaggagt gttttgatga
    10381 gtaaaattat tctagtaaca ggaggagcta ggtcagggaa gagtaacttt gcagaaaaac
    10441 tatgttttga cagaaataat aatacagctt atatagcaac ctctattcca tttgatgatg
    10501 aaatgaaaga tagagtaaaa aaacatcaag agaatagacc tagaaactgg aatacttatg
    10561 aaatatatga agatatatat tccataatta aggagatata taaagaacat gaaactgtta
    10621 tactagattg tgtaacatta ttagtaaata atctaatgtt tgaacacaat ctaaatatag
    10681 aagaatctag tcaagaagaa ataaacaaat tagaacaata cataaaagaa caagttgtca
    10741 aacttattga agaaatagaa aaaacaaatc tatactttgt atttgtaacg aatgagttag
    10801 gtatgggaat tgttcctggg aataaactta gtagaattta tgctgatatt gtaggtcgta
    10861 taaatcaata tatagcttcg aaaagtaatg aagtttattt tgtagttagt ggcatagcta
    10921 tgaagataaa ggagtagtat gcaattatga aaagatttat tttaatacta cagtttttga
    10981 caaggatacc tataaaatta aatgtaggat ttgatgatga attttataag tcaatagtat
    11041 attttccact tgtaggtttt gtcataggta tattatccta tttaatagga tggatttcta
    11101 tgcttttatt tgaaccattt atagcttcta taataataac tctagctggt gtcttaatta
    11161 ctggaggact tcatatagat ggattaggag atacatttga tgcaatatat agttataggg
    11221 ataaagaaaa gatgcttgaa ataatgaaag attcaagatt aggtacaaat tcacttctag
    11281 caattatgtt tgtactttta ttaaaggttg gattcgttta cgatataata agtaataatt
    11341 cattgtgggt tataattttt atgccaatga ttgcaaggtt aggagtaatg ttattgacat
    11401 ataaaactgt gacaccaaga gaaaaaggaa tgggaaattt atttatagga aaattaacta
    11461 caagtatgtt aataactgct ataatataca cattattgat agttgctctt attactaaat
    11521 ttatattttt attacctaat atagtgttga ttaaagtatt aggttcgatt atagtggttt
    11581 ttgtatttat aatattattc aaaaaacata tttataaaaa aattgacgga gtaacaggag
    11641 atatattagg ctgtggaata gaactttcag agcttgtata tttaatttac atatatttgt
    11701 taatttttat gtttttttaa agtaaaaagt ggtgataata tgataagatt aatattaata
    11761 agacatgcac tgacaaatga taataaaaaa ggaagattat caggccatat aaatagttgt
    11821 attagtgaag agggcaaact acaaataaat aaaattacca gatatctgag taatgaaaac
    11881 atagataagg tttatacaac accatctaca cgtactaaag atacagtaga aaaaatatca
    11941 aaattgaaat tattagaaat agaagaaaaa gaagctttaa gagaaattag ttttggagac
    12001 tttgaaggaa gaacttttga agaaattaaa atcaaatatc caaatgagtt tgaaaaaatg
    12061 ataaaagaag gtaacaacta tagatatcca aatggagaaa gtttgataga ctcttataaa
    12121 agagtggcaa aagaaattga taatataatt ttagaaaata atagtaattt agatacaaag
    12181 acaatactta tatgttctca tgcaggaaca ataagaaata taataacaca tttgatttct
    12241 ggtagttata agtatcactg gaactttaaa attgataatg catctattac agttttagaa
    12301 attgatggtg gatttgctgt aatagataaa atgaatttta cagacttcat ataatttaat
    12361 caaaaccaaa cattacaatc tgtgtttgta atatatctgt aaaattagca atgaaaatag
    12421 aatattagat taaaattagg ttcttaatta gatgttaaaa gggaaaaagg ttagatgcct
    12481 ttgcagcccc cgctactgtg aaaccaacga aactataaat accactgtca gtttgatggg
    12541 aaggttatag aagtaggatg aagttaagcc aggatacctg cctaatttaa tttaaaacaa
    12601 gaggtcttcg gggtgaaaga tttttaatta atatatataa aagtcctatc tttagaagta
    12661 ttaggacttt ttttaatttg ttttttagta aattttaaca aggagggatg gaaagtgggg
    12721 aaaaaaataa tgctacaagg aacagcttca aatgtaggta aaagtgtctt agtagcaggt
    12781 ttgtgtagaa tatttaagca agatggttat actgttgctc cttttaagtc acaaaatatg
    12841 gctttaaatt cttttataac taaagaagga ttagaaatgg gaagagcaca agtatttcaa
    12901 gcagaggctg cgaaaataga gccaatagct gatatgaatc cagttttatt aaaaccttct
    12961 ggaaataata aaagtcaagt tattgtaaga ggtaaagttg taggagaaat gccttcttct
    13021 aagtatcatg attataaatt ggagttagta gatgtgttga aggaaacatt taatagctta
    13081 aattcaaggt atgatattgt agttatggaa ggggcaggaa gtaatgcaga gataaactta
    13141 aaagatagag atatctctaa tatgggtatg gcagaaatag ctgatgcacc tgtaataata
    13201 gtcggagata ttgatagagg aggcgtattt gcctcattag caggaacaat gcttttatta
    13261 aatgaagatg agaaaagaag agtaaaaggt gtaataatca ataaattcag aggaaaaaaa
    13321 gagcttttac aagatggtgt aaagatgcta gaagacatca taaaagtacc tgttatagga
    13381 gtagtacctt atacagatat aaaaatagaa gatgaagaca gtgtaactac gagatttaag
    13441 aaaaaaatga ataaaagtga tatacacata gaaataatta gaaccccaca tatgtcaaat
    13501 tttacagatt ttaatatatt tgagacacaa gaagatgtaa gtataagata tgtagactat
    13561 ggagaatctt taggaaatcc agatatatta ataatacctg gaactaaaag tactatagac
    13621 gatttaacat atataagaaa aagtggtctt gaatcgcaaa tcaaaaattt acatagtcaa
    13681 ggtaaattga tatttgggat atgtggtgga taccaaatgt taggtaaaaa attaaaagac
    13741 ccttatcatg ttgaaagtga aattgaagaa gttgatggaa taggtctttt agatacagaa
    13801 acaacgtttg agaaagaaaa gactacaaca caagtagaag ctactattgt tgaatgtatc
    13861 agtgaataca tgaataacct taagggaaaa aaagtaaaag gttatgaaat tcatatggga
    13921 attacaaagc gaggtaatga tgtaagaagt ttagatttaa taactaaaaa acttgataaa
    13981 gaagtgaatt atactgaggg aagtgtaaat aaagagggga atgtaattgg gacttatctt
    14041 catggaatat ttgatgaaat aagctttaca agagcaattt taaacaatat aagaaagaaa
    14101 aaaggtttag gagaattaga aagcaaagta aactctttta atgaatttaa ggataaagag
    14161 tatgataagt tggctgattt tttaagagaa catttagata tagagaaaat atatgagatt
    14221 atgaaataat gatgaccttt atttataatt taggtaggag gtgtattatg aaaaaaattt
    14281 taattgcggg tactaatagt ggagtaggta aaacaacaat atcattagga ataatgcaag
    14341 ctttggttaa gagaaatatg aaagtacagc catataaagt agggccagac tatatagacc
    14401 cttcatatca cacttttata actggaagac attctagaaa tttagattct tatatgttag
    14461 atgatgaaaa aataaagtat atagtaaata agtcctcaaa agatgcagat atttcagtaa
    14521 tagaaggtgt tatgggctta tatgatggct ttggaataga tttagataat tgtacaagtt
    14581 cacatacttc aaaggtatta aagtcacctg ttatattagt tataaatggt aaatctatgg
    14641 cagcatctag tgctgctatg gtacttggat ataaggaact agataaagat gtaaatataa
    14701 agggtgttat agtaaataat gtaaagacta atagccatta tcaaataatt aagagttcta
    14761 tagaaaaata ttgtaatgtt gaagtattgg ggtattttcc tccaaataac aaattctctt
    14821 tggaatctcg acatctagga ttagttccta gtgtagaaat gaaatcacta agggaaaaat
    14881 tttatacctt agcagatgaa attgaacact atatagatat tgacagaata attgaaatat
    14941 cagaaagtga tgaatttgag agtgattttg attttcaaac tttgatagaa aataataaaa
    15001 taaaagaaca tgttaataat aaatcaatag ctatagcata tgataaagca ttcaattttt
    15061 attatagaga aaatatagag cttttagagg aacttggttt agaaataaac tattttagcc
    15121 ctcttgaaga tgtatcagtg ccaaatgcag attatattta tattggagga ggttttccag
    15181 aaatatttgg aaaagaatta gaagcaaatg agtcaatgag aacttccata ttagaagcac
    15241 ataacaataa tatacctatt tatgcagaat gtggaggact gatgtaccta ggtgagaaac
    15301 tattgaatca agaagaacaa gaatttaata tggttggaat atttagtgga attagtaaga
    15361 tgacatcttc tttaaagaga tttgggtact gtattggtgt agccaaggaa aatactcttt
    15421 tatctaaaaa aggggaatcc attaaagggc atgagtttca tcattcaata tttgaaagtg
    15481 atgaaaaatg tgtatattca atgagaaaag aaagagatgg aaatatagta gaagagtggg
    15541 atggtggtta tagtaaagga aacactttgg ctacatatct tcatactcat ttttataata
    15601 acttaaattg tatagaaaat tttttgaaaa aaggaaatga ataaatgagt attttatcta
    15661 tttatatagg atatgtgaca gacttaattg taggagaccc atattctttt cctcatccag
    15721 tgagatttat tggtaaactt ataaatttta ctcaaggtat aataagaaag gtctctaaaa
    15781 atgataggca gttaaaattt gggggattta tattatggtt tataaccgtt ggaataacat
    15841 atttaacaac ttatgctata gtgaagttat ttagttttaa tatattattg agtattgtag
    15901 tgaatagttt tataatttat acaacactag ccacaaaatg tttaaaagac gaagcattaa
    15961 aaatttataa tgtattaaaa actgaagata tagaaaaatc taggattcag ctatcttata
    16021 tagtaggtag ggatactact aatttaagtg aacctgaaat aataagagca actgtagaaa
    16081 ctgttgcaga aaatactgta gatggtataa ttgcgcctat gttttatgca tttattggag
    16141 gagcaccact tgctatggcc tataaagcta tcaatacatt ggactcaaca gttggatata
    16201 aaaataaaaa gtataaagat attggttttg catctgctaa gattgatgat atagccaatt
    16261 atataccagc tagaatatct gtcattctta tgacaattgg aagtttcttt ttaaattata
    16321 actataggaa ctgttttaaa atctctatta gagatagaaa gaatcataaa agtccaaatt
    16381 gtgctttttc agaaggtgca gtatctggtg ctctaggaat acaactagga ggtaccaacg
    16441 tttattttgg ggaaaaagta tataagccaa caatagggga taagcttagg gagatagata
    16501 aagaagatat aattaaaact aataaaatta tgtatgcatc ttcatttgta tctatgttgg
    16561 tatttacact aatatatgtg ttatgtagga tgattttaat taacttaatt atataaatta
    16621 ttgtttgtta acttaagggg gacatagatg aaggatttag ggcatggtgc caatgtagat
    16681 gaaatggcaa gattatatgg gaaaaaccca aaagaaataa ttgattttag ttcaaacata
    16741 aatccaaatg tattaccaaa tttagagaga tacattttaa aaggattaga agagtgtagg
    16801 aattatccag atataaatta tacaaacttg agagaaaata tatcaaaata tatagatata
    16861 aatccagact ttataatacc tggcaatggt gcaacagaag tgatttattt gcttatgaag
    16921 agtattaaaa aaaaattggc tataataaat ccgacttttt cagagtatag aagaagtgct
    16981 gagttaaata atttagatat tatagattta gagttagatt tagaaaataa ctttaaactt
    17041 aatatagata taataaaaga aaatataaaa agatttgata gtttatttat atgtaatcct
    17101 aataatccga gtggtaatgt ccaagattta aaggaacttg ttcatttact agataaacat
    17161 aataaggtac taataattga tgaaactttc atggaatttg ttgaagatga aagtaaatat
    17221 agtttggtta aatatataga atcaaataaa aatatattta taattaaagc tgtgactaag
    17281 ttttttggta tgcctggttt aaggctagga tatggtctta caagcaacac tgaaattatg
    17341 aacaaaattt atgaacacaa agagccatgg actatcaatt cttttgcaga tatactatcc
    17401 aattttattt ttgaagataa agagtatatt aaaaatagta aagaatatta catagaagaa
    17461 agaaaatata tgttacaaga gttgagaaac ataagaaata taaaggtcta tgatacagat
    17521 gctaatttta tcttgataag aatatataaa aaaacaacta aagaattgaa aaaagatttg
    17581 tttaagcaag gaaacatatt agttagagat gcatctaatt ttataggatt agatgatagc
    17641 tttataagag tggctataaa atctcatgaa gataacaaaa ttcttataga aaatatgaaa
    17701 aatttattgg gtgattaaaa tgaaatctta tggaatatgc ccagcatctt gtggagaatt
    17761 tgtacaaggt ataattgatg atgaagaata tttatgttca tatgcaatag atatgtattc
    17821 taaagtgtat attgaagaaa aattagtaga tataaattta gggagatata agtcaaggtt
    17881 agcaattgaa aaggtatttg aaaaatttaa tctacctaaa aaatatacta aaaatatttc
    17941 tttaaatata aatagtaaaa tacctgttgg aaaaggaatg gcaagttcaa ctgctgatat
    18001 aggagctact ataaaggcaa cattatcatt gatagataaa gatttaagta gcgaggagat
    18061 ttctaaatta gcagcagaaa tagaaccaac agattctata tttatagata aaaatagtat
    18121 atttaatcct ctaaatggaa ctgttataaa atacctagga aatctgacca atgcgaaagt
    18181 tgtaatactt gaaccaaaca aagtactaga tactatgaaa attagattga ggcaagatta
    18241 taataaatta aaagtagaga ataaagaagt aataaaaaaa tcatttgctc ttttagaaga
    18301 aggattgaaa aaaaataatt tatctctagt tggagaggct tgtactttga gtagcttagc
    18361 aaatgagaat atagaaaaaa aagagtattt aaatgaaata ataaaaatat caaaaaaata
    18421 tggagcatat ggagtaaata ttgctcacag tggaactgta gtaggtattt taattgataa
    18481 gtttatgaat gataaaaaga tgatagatgc cttatgtgaa tctaacataa attctgtcta
    18541 taataaaatt tatacacaaa acataattaa tggaggaatt aagggggaaa tagaatggaa
    18601 tacataaaga atcctatgaa aatagaagaa aaaagttttg aattgataca agaaataata
    18661 gacgaaatta gaccagatta taaatttaag aataatatcg aagaaaaaat aataaaaaga
    18721 gctatacata ctactgctga ttttgattac ttagatatat taaaaatatc agaagaagca
    18781 gttgatagta taatagatgc attaaaaaat aatgcaagta tttatactga tacaaatatg
    18841 gctcttagtg gtataaataa aagaaagtta gaatcattag gatgtaagta taaatgttta
    18901 gtggcagatg atgaaactgt aaaattagct aaagaaaaag gaattactcg ttctatggca
    18961 gcagtagaaa ttgcagctaa agaagaagga agaaaaatat tcgttttagg taatgctcca
    19021 acagctttat ataaagttat ggagatgaaa tcagaaggta gattagattt agatgcagtt
    19081 ataggagttc ctgtaggttt tgtaggagca caagaatcaa aagatgaagt tgaaaaaaca
    19141 gatataccat atataatttc taaaggtaga aaaggtggca gtaatttagc agcagcaata
    19201 gtaaatgcta tattatatac tatgtaggta cctatatgga agaatatgta tatatagatg
    19261 gaaaaaaata tagaagagga tatactacag gttcatgtgc aacaggtgca tctaaggctg
    19321 ctgtatatat gttgattaca aaaaatagaa tcaatactat aaatatagat actcctaagg
    19381 ggataccatt actactaaaa gttgataata taaatatttc tgatacattt gtggaatgtt
    19441 ccataaaaaa agatggtgga gatgatatag atgcaactca taccatggat atttatgcaa
    19501 gagctgaaat tgtagctaaa aatgataaga acaaagggta tcttactcta aaggatatag
    19561 ataatttaag caccaatagt gagtgtaaaa gtgagctcta taagtttatt agggtttatg
    19621 gcggaactgg tattggtgtg gttacaaaaa aaggtcttag cgtggatgtt ggtaaacctg
    19681 ctataaatcc gactccactt aaaatgataa atcatgaaat aagaaagctt ataggagata
    19741 attttgaatc tatacttgga aatgataaag ttttaaaaat aacaatcttt gctccacaag
    19801 gagaaactgt agcaaaaaaa acatttaatc caagattagg tatagttgga ggaatatcta
    19861 taataggtac aactgggatt gttgaaccta tgagtgatga gggttggaag aagtctctat
    19921 ctatagaact tcagatgaaa aaagaacaag gcttagataa aataatattg gttccaggaa
    19981 atcatggaga acagtttata agagaaaaat taaacttaga tattaagtat gtagttaggg
    20041 taagtaattt tataggctat atgattaaag aagctcaaag aataggatat aagaaaatct
    20101 taatggcagg tcatataggt aaatttataa aagtatctgc tggtattttt aatactcata
    20161 gtaaggtagc agatgctaga agtgagatat tagtggcaaa cttagcttta atgggagcta
    20221 gatacgaatt tttaaataaa ataaatcaat gcgtaactac tgaagaagct gtagagctaa
    20281 taaataatag tgaatataga gaagtatata atatattaag taataaatgt agggagagag
    20341 taaagcaata cttgaatgaa gattcagatg atattgatgt ggaagtaatc atattttcaa
    20401 tggataagtc tttattaggt aaatcggata atacagatga tttagtggag gtctttatat
    20461 gataaacata ataggtctag gacctggtaa cttagactat ataacaaaaa aaggtgaaaa
    20521 tttaatttcc acttctgatg tattaattgg tggtaaaaga aatttagagt ctataaaaaa
    20581 ttttgaagga gaaaaaatag ttctggattc taatttaaga gaaattatag agtacataaa
    20641 taacaataaa gaaaagcaaa tatcaataat agcatctggg gacccactaa tatatgggat
    20701 aggtagatat ctatctaaaa atattgataa taagatgtta aacatggttt ctgggattag
    20761 ttcattacag tacatatttt caaaaatata tgtagatatg aatgatgtat atattacaag
    20821 tagtcatggt aaagtaccag attttgatta catattatca cataaaaaag tatgtatggt
    20881 tacagattca aagataggac caaaacaaat aagtagagaa ataatagata gaaatctaaa
    20941 taaaattatt gttgtaggag aaaatctgtc atatgacaat gaaaaaataa caatagcaaa
    21001 accagaagag ataataagaa tggacaattt tgatatgaat gttgtggtga ttttagatga
    21061 ggaatagtga atttataaca ggtaaagttc caattactaa ggaagaggtt agagcaatat
    21121 caataagtaa gttaaattta gtaaatgcta aaaacctcat agacataggg gctggtacag
    21181 gaagtgtaag tgttgaagct gcatataatt atcctaattt aaaggttata tctatagaga
    21241 aaaatgatga tgctatagag cttatagaga aaaataaaaa aaaattcaac ttgaaaaata
    21301 tagaagtgat aaaaggatat gctccaatta atctagggtt aaatgttaaa gttgagtcta
    21361 tttttcttgg gggaacagga aataatctgg aagaaataat agaatggtct aaaaaaaatc
    21421 tcgttacagg aggaagactt gtagctaatt ttataataat agatacattt aatcaaactt
    21481 taaaactgct taaaaagcat ggatttaaag aaatagatgt atgtgttttg aatgtttcta
    21541 aactagagaa actaggaaaa ggtgaatatt ttaaaccact aaatcctata tatataatat
    21601 cttgtgaaaa aggggaaaat gatgatgaat aaagtacatt ttgtaggagc aggacctgga
    21661 gataaagagc tgataacatt aaaaggatac aaattattaa gtaatgcaga tgtagtgata
    21721 tatgcaggtt cacttgttaa tcctgaactt ttggaatact gtaaagaaga ttgccaaata
    21781 cataatagtg cacatatgga tttacaagag ataatagatg ttatgagaga aggtatagaa
    21841 aataataaat ctgttgtaag actgcaaaca ggtgattttt ctatatatgg ttcaattaga
    21901 gaacaagtgg aagatttaaa taaattgaat atagattatg actgtactcc tggagtaagt
    21961 tcttttttag gggcagcatc atctttaggg gttgaatata cagttccaga aatttctcaa
    22021 agtgtaatta taacaagaat ggaaggcaga actccggttc cagagaaaga atcaatacaa
    22081 tcatatgcta agcatcagac atctatggta atatttttat ctgttcaaga aattgagaaa
    22141 gttgtgtcta aattactgga aggtggatat ccaaaagata ctcctatagc agttatatac
    22201 aaagcaactt gggcagatga aaaaatagtt aaaggaactt taagtgatat agctgtaaaa
    22261 gttaaagaaa acaacataaa taagactgct ttaataatgg ttgggagatt tttaggagaa
    22321 gagtataata attcaaagtt atatgataag gatttcaaac atgaatatag agggtaatat
    22381 tgctataatc tgtattacag aaaatggaaa aaaattagct ctaaagataa attctctagt
    22441 aaataactgt gttatatatc aagtaaaaaa taaaaaaagt aatttagttg ttgaagaaac
    22501 atgtgtgcat acagttcaaa agaagttaag tgattttgta ggagaaatat ttagcaaatt
    22561 tgattacata gtatttataa tggctacagg tatagttgta cgaagtatag caccatacat
    22621 aataagtaag tttagtgacc ctgcaatttt ggtaatggat gaaaaaggca aaaacgtaat
    22681 aagtttattg agtggtcata ttggaggggc aaacagcttg acacagtaca taagtagtct
    22741 tattgatgct aatccagtaa taacaacagc tactgatata aatcaaaaag cttccttaga
    22801 tatgatagca aaaaaactaa atgcacatat tgataatttt agagaaagtg taaaagaagt
    22861 aaattctatg ctagtgaata ataaatgtgt tggattatat gttgatgaag gatatcaagt
    22921 tgatactagg ggatttaaac ttatagaaag taaggatgag ttaagagata gtttttcaaa
    22981 cacatatata gaaaaattag ttgtagtaag caataaaata aattgtattg aagatattga
    23041 gaatttaaac ctagataata aatttgaaca ttgctatagt acccaaaaaa taataaaggt
    23101 aattccaaga gatatagtga taggaattgg ctgtagaaga aacacagata gtgaactgat
    23161 gtataattca cttgaagagt ttcttaataa acaaaatata gatattagag caataaacac
    23221 agttggaagt atagaactga aaaaagacga aaaagcaata attgaattat caaaaatatt
    23281 gaaggtgcca tttaaaacat tttcagtaga tgaaatatct aaggttgata atctttttga
    23341 taaatctgat tttgttaaaa aaaatgtggg tgtgtattca gtagcagaac ctgtagcata
    23401 tcttttgagc aaaggtaatt tgttagttga aaaacataaa tataaaggaa taactttttc
    23461 agtggggaga ataaaaatat gatatacgtt gtaggaattg gaccaggaag taaagataca
    23521 atgactttag aagcaataaa agcaatagaa gattctgagg ctattgttgg atataagact
    23581 tatataaaac taatagaaga atttatacaa ggtaaagaaa taatccaaaa tggtatgagg
    23641 caagaagttg atagatgtaa acaagcagtg gaggaagcaa aaaaaggaaa aaaagtagca
    23701 gttgtaagca gtggtgatgc tggaatatat gggatggcag gtcttatttt agaattaata
    23761 tctaaagaag atgaagatat aaaagtaaag gtaattccag gcgttactgc aagtattggt
    23821 gcagcagcta tacttggagc tcctattatg catgatttct gtcatataag tttgagtgat
    23881 ttaatgactc cgtgggaagt aattgaaaaa agattgagat tggctgctga agccgatttt
    23941 gtaatttgtt tgtataatcc aagaagtaag ggaagaagtg aacatctaag caaagcattc
    24001 aaaataatgg gtgagtttaa aggtggagat acacctgttg gagtggtaaa agatgtaggt
    24061 agagaaaaag aagaaaagtt tgtatgtact tttgaaaata tggactttga aaaagtcgat
    24121 atgactacaa tggttataat aggaaataag tctacctata ttaatggtga attgatgata
    24181 actccaagag gttatacagt atgattttaa ttttaggagg aacttcagat agtttatcta
    24241 tatgtgataa gataaatgaa ctgaaaaatc aaccatatat cctttcagtt actacagatt
    24301 atggaaggga acttgcacaa caacatgcag agaatgtaat tttaggtaag cttggaaaag
    24361 aagatatgct aaaatttata aaagataata atatagtaaa aataatagat gccactcatc
    24421 cttatgcagt tgaagtatcc aaaactgcta ttacttgtgc aaaactattg aatatagatt
    24481 atataaggtt tgagagaaaa tctttaattg aagaaataga ttatgataat aaatttatag
    24541 tagatacaat tgaagaagcc tgtgaaatag caaataaaat agggacaaat atattcatag
    24601 gaacaggaag taaaaatttg aacagattta tagaggaaat aagtgataaa aatttagttg
    24661 ttagagttct tccaacgagt gaagtcatat taagttgtga aaaattaggt ttaagtgcag
    24721 ataatatagt agctatgaaa ggtccattta gccagtatat aaatgaagaa acatataaac
    24781 attacaatat agatttagtc ataacaaaag aaagtggaat agctggtggg ttcttagaga
    24841 aggtaaatgc atgtaaaact ttcaatatac cagtaataat tataaaaaga gaatcgatta
    24901 actacgaaaa tgtatttaat gacataaata aaattaaaga catacttgtg taatttttag
    24961 gaggagaata tatgaagaaa gccttattag tagtgagttt tggtacaagc tatcatgaaa
    25021 cgagagaaaa gacaatagat atatgtgaag ataaaataaa aaattcgtta aaaagccatg
    25081 atttttttag agcttataca tcaaatatga taataaataa aataaaaaaa agggatggaa
    25141 tagaaataga taatccaata caggctttag ataaaatata tgaacaagga tatgatgaag
    25201 taataatcca aacacttcat attatatgtg gcgaagaatt taacaaatta aaagaacaag
    25261 tagatggata ttcttctaaa ttcaaaaaga ttgtattagg aagacctttg cttacacata
    25321 ttgaagatta tcaggaagct gtagaggcaa ttaagcatca aattcctcat atggaaagta
    25381 atgaagcagt agtatttatg ggacatggaa cacttcatga atcacattca gcgtatccag
    25441 cattggaata catgcttagg gataatggaa taaatgctta tgttggtaca gttgaagggt
    25501 atccagaaat agaacatgta ataagaagat taaaagaagg aaatataaaa actgtaaatt
    25561 tgatgccatt tatgttagtt gcaggagacc atgctataaa tgatatggca ggagatgaag
    25621 aagattcttg gaaaacaata ttagaagaaa atggatttaa cgtaagagtt catttaaaag
    25681 gactaggaga aaattcttat atacaagata aatttgttcg tcatgctgta aaatgtgcag
    25741 agaatgcgaa gttctatggt ataggagcag ggccaggaga tggttcttta cttacgataa
    25801 aagcggttaa tactttaaag aaactggata ttctatacac tccagaatca aaaaagggtg
    25861 gagatagtct agctttatca atagttagtg aatatttacc tgaatcagtg gaaataaagt
    25921 ctaggcattt cccaatgagt tttgatggga atgaaaaaag tttagcttgg gacaatgtgg
    25981 cacaagaaat tatatcagat gttaatgatg gtaaaaatgt aggatttgtt acattaggag
    26041 accctatgat ttatagtaca tatgtatata taatgaaaag aatattagga gaggtagaag
    26101 tcgaaactat accaggaatt tcatcatttt ccaatatagc atctaatcag aattttccat
    26161 tagtcatgga taaagaagct ttagtggtaa ttccttgtac tatggaagat gataagatag
    26221 agtatgcttt gcaaaattat aattcaatag tattgatgaa agtatataaa aattttaaag
    26281 aaataataaa aaaattagaa aagtatgatt taatagaaca tgctatttta gtaagtaatt
    26341 cttcccaaga aagcgaaaat gtgtttacag atttaaaaga agcgcacctt gaagataaga
    26401 tatcttattt ttctactata ctagtaaata aagataataa aataaaatag gtgaaagcaa
    26461 tatttaaagg agacttaata tgaatatagt ggttggaact aggggtagta atctagcgct
    26521 tatacaaaca gaatgggtaa taaatgaatt aaaaaagaag tatccagaaa ttagttttga
    26581 gattaaaatc ataaagacaa aaggagattt aattcaaaat gtttcactgg ataaaatagg
    26641 tgataaagga ttgtttgtca aagaaataga gcaacaatta ttagatggaa aaatagacat
    26701 agcagtacat agtatgaaag atatgccttc ttatttagca gatggactaa aatttgctca
    26761 tacgcctaag agagaagacc caagagatgt tctaatactt agagaaggat ataaaaattt
    26821 agatgatttg cctcatggag cagtaatagg aacaggaagt aaaagaagga agtttcaatt
    26881 attgaaacaa agacctgatt taaatatagt acaagttaga ggtaatgtgg aaactaggat
    26941 acgaaaaata aaagatgaaa acatgcatgg tattgtgttg gcagcatcag gaataattag
    27001 agctaattta caagataaaa taagttctta tttacctgtt gatgttgtta ttccagcacc
    27061 tgcccaagga gctttagcta tagaaattag aagcaatgat agtacgatag agggaatagt
    27121 aaattcactg aaggatgaga atacagaaat ccaaatattg gctgaaagag gttttttaga
    27181 tggtgtaaat ggaagttgtc atattccaat ggcagcttat tgtgaaataa tccaagataa
    27241 aatacatctt actggcttat atggagattc agaaggaaaa aaagtagtaa tcaagtctat
    27301 agatggagat atttcttctc ctagagagtt aggtttaaag ttagctaaac ttgtattgaa
    27361 ggagtatgag aattatgaag ggtaaggttt acttagtagg ggcaggacca ggtgattata
    27421 aactcatgac tttaaaaggt ttagagtgta taagaaaatc ggatgtaata gtatatgata
    27481 gattagcaaa tagtagttat ttaagagaag ctaagcctga ttgtgaactt atatatgtag
    27541 gcaaggcatc aagtaatcat attttgccac aagaagatat aaatagaatt atagcagaaa
    27601 aagctaaaga aggaaaaatt gtaactcgtc ttaaaggagg agacccttat gtatttggca
    27661 gagggggaga agaagcagag actttagtag atgaaggtat agaatttgaa gtagttccag
    27721 gtataacttc tgctatagga ggtttatgtt atgcgggtat tcctataaca catagagacc
    27781 atgcttcgtc atttcatgta ataacaggtc atttgaaggg tgatgatagt ggagaattaa
    27841 actggaatgc tcttgcaaat aacaaaggta ctcttgtgtt tttgatggga atttcaaacc
    27901 tagagaaaat aagtgaaaac ttaatgaaag aaggaaaaga taaggataca cctgttgctc
    27961 ttataagttg ggcaactaga tataatcaaa gagttgttac atctacactt gaaaatgttt
    28021 atgaaactgc tattaaagaa gaagtaaaac cgccaactct tatagtagtt gggagtgttg
    28081 taggacttag agaaaagttg aacttctttg aaagtaagcc tttatttgga aaaagtattg
    28141 ctgtaactag gtctagaaat caaaatagtg tattggtaga aaaaataatg gatttaggtg
    28201 gaaatcctat agaaatacct actattaagg tagaaaaaat acaaaataat attaacttag
    28261 aaaatgaaat aaaaaatata aataagtata attatctaat actaactagt aaaaatgccg
    28321 tagaaatctt ctttgaaaag atatatgaaa tgaattttga tttaaggata ttatctaatt
    28381 taaaggtatg tgctataggt tctgctacat caaatgagtt gaaaaaaaga ggaattatag
    28441 cggatatagt accaaaaaaa tttgtagcag agtcactgta tgaagagtta gcgcctattt
    28501 taaatgatga cgataatata cttataccta gagcagaaaa tgctagagat tatctggttg
    28561 acaaactcaa ggctatttgt gaagtaacag aagtacatat atatagaaca gtaatagatg
    28621 aggagaaaaa aggtgagata ttagatattt taaattctaa tgacatagat tatataacat
    28681 ttactagttc ctcaacagta aataatcttg ttgaaattat aggaaaagaa aatattaaaa
    28741 tattagaaaa aataaaaaca ctatctatag gtcctataac tacagagaca ataaatagtt
    28801 tgggaataaa attgtacaaa gaatctgcta catctactat agattcgtta gtagatttaa
    28861 ttataaaaaa ttaattttgg aggcttgaat atgttaagaa gaccaagaag actaagagca
    28921 agtaaagcaa tcagaaattt ggtaagagaa actaaggtga atgtagaaga cttaatatat
    28981 ccactgtttg tagtagaggg ggaaaatata aaagaagaaa ttaaatcttt accagatgtc
    29041 tatcatttat caatagatat gctagaagac gaaataaaag aaataagtga gttgggaatt
    29101 gaacatataa tgttatttgg tgttccagat gaaaaagatg agtgtgcttc acaagctttt
    29161 aatgataatg gaatagttca aaaggcaatt agaaaggtaa aagaaataaa ttcagatata
    29221 aatgtaataa cagatgtctg tatgtgccaa tatacaagtc atggacattg tgggatttta
    29281 actgatactg gttatgttga taatgataaa tcattggagt atttggctaa aatatctgta
    29341 agccatgcta aagcaggagc tgatatggtt gctccatctg atatgatgga tggaagaata
    29401 caagcaataa gagaagcttt agatgaaaat ggattagaaa atgttggaat catggcttat
    29461 agtgttaagt atgcttcaaa ttattatgga ccttttagag aggcagctca ttcagcacca
    29521 tcttttggag atagaaaaac atatcaaatg gacccagcaa atagcaatga agctataata
    29581 gagactgaac ttgatatatt ggaaggtgca gatatactta tggttaaacc tgcattatca
    29641 tatttagata ttataagaag agttaaagat aattttgact tacctctagc agcttataat
    29701 gtaagtggag aatactctat gcttaagttt gctgtgaagg aaggtttatt aaatgaagat
    29761 gtaattcttg aatcaattac atctataaaa agagcaggag cagatataat aataacttat
    29821 tttgctaaag acattgctaa actgttgaaa aaataaattc tagcagtata aagaatatca
    29881 aaaagtatag gaattgtaat taactattat acagtagttt tgtttagaca tttattgaag
    29941 aataatagaa actaatttgc aatttctata ctttatttat gtaactaaat aaaatttgga
    30001 ggtagaagat gctatatcct attaatttaa aattagatga gttagatgta gttataattg
    30061 gtggtggaga agttgcatat aggaaatgta aaaatttttt agagtttaac aaaaatgtta
    30121 ctatagtttc taaacaaatt ttaaataaat tttatgattt aaaaggaaat ataaaaataa
    30181 taaaagatga ttataaagaa atatatataa aagatgcttc tgtaataatt gctgccacaa
    30241 ataatagaga gttaaatatg gaaataggat tatattgtaa agcaaaaaat aagctagtga
    30301 atgtagttga taatatagaa atatcgaact ttactgttcc atcatatata aaaagaggag
    30361 atttacttat aagtgtgtct actgggggca agagtccttc attatcatca aagataaaaa
    30421 aagaaattga agagagatat actgaagatt atgaggagta tttaaatgta ttaggagata
    30481 taagaaaaaa ggttataaaa aaatacgaag ataaaagtaa aagaaaagat gtgcttaata
    30541 tgttggtaac acttgaccta gaagaactta agaagtttga tatataaaaa ataaaataaa
    30601 aaatgttaaa aaggtgttga cggattattt ttaagatgat atagtattac ttgtccttaa
    30661 gaaaagggca aacataaaaa atgaactttg aaaattaaac agtaggttaa tttat
//
LOCUS       DAELTA010000030        30165 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.030, whole genome shotgun sequence.
ACCESSION   DAELTA010000030 DAELTA010000000
VERSION     DAELTA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.030"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            200..1528
                     /locus_tag="KPG13_001185"
     CDS             200..1528
                     /locus_tag="KPG13_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0984923.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
     gene            2281..3114
                     /locus_tag="KPG13_001186"
     CDS             2281..3114
                     /locus_tag="KPG13_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0984924.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            3207..4139
                     /locus_tag="KPG13_001187"
     CDS             3207..4139
                     /locus_tag="KPG13_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0984925.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            4191..4673
                     /locus_tag="KPG13_001188"
     CDS             4191..4673
                     /locus_tag="KPG13_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0984926.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            4685..5554
                     /locus_tag="KPG13_001189"
     CDS             4685..5554
                     /locus_tag="KPG13_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0984927.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            5597..6352
                     /locus_tag="KPG13_001190"
     CDS             5597..6352
                     /locus_tag="KPG13_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0984928.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            6394..7215
                     /locus_tag="KPG13_001191"
     CDS             6394..7215
                     /locus_tag="KPG13_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBG0984929.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            7220..7981
                     /locus_tag="KPG13_001192"
     CDS             7220..7981
                     /locus_tag="KPG13_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG0984930.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    8142..8399
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8512..8751
                     /locus_tag="KPG13_001193"
     CDS             8512..8751
                     /locus_tag="KPG13_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0984931.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            9055..10050
                     /gene="ilvC"
                     /locus_tag="KPG13_001194"
     CDS             9055..10050
                     /gene="ilvC"
                     /locus_tag="KPG13_001194"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG0984932.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            10103..11791
                     /gene="ilvB"
                     /locus_tag="KPG13_001195"
     CDS             10103..11791
                     /gene="ilvB"
                     /locus_tag="KPG13_001195"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG0984933.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(12152..13282)
                     /locus_tag="KPG13_001196"
     CDS             complement(12152..13282)
                     /locus_tag="KPG13_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0984934.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITVEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(13522..14026)
                     /locus_tag="KPG13_001197"
                     /pseudo
     CDS             complement(13522..14026)
                     /locus_tag="KPG13_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(14289..15878)
                     /locus_tag="KPG13_001198"
     CDS             complement(14289..15878)
                     /locus_tag="KPG13_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0984935.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLITFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(15902..16465)
                     /locus_tag="KPG13_001199"
     CDS             complement(15902..16465)
                     /locus_tag="KPG13_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG0984936.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            16618..16839
                     /locus_tag="KPG13_001200"
     CDS             16618..16839
                     /locus_tag="KPG13_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984937.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            16842..17657
                     /locus_tag="KPG13_001201"
     CDS             16842..17657
                     /locus_tag="KPG13_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HBG0984938.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            17704..17913
                     /locus_tag="KPG13_001202"
     CDS             17704..17913
                     /locus_tag="KPG13_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984939.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMSKSEKRKISEITKERIRK"
     gene            complement(17923..18297)
                     /locus_tag="KPG13_001203"
     CDS             complement(17923..18297)
                     /locus_tag="KPG13_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984940.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            18373..18570
                     /locus_tag="KPG13_001204"
     CDS             18373..18570
                     /locus_tag="KPG13_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984941.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            18601..18993
                     /locus_tag="KPG13_001205"
     CDS             18601..18993
                     /locus_tag="KPG13_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0984942.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            18974..19123
                     /locus_tag="KPG13_001206"
     CDS             18974..19123
                     /locus_tag="KPG13_001206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984943.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            19194..19631
                     /locus_tag="KPG13_001207"
     CDS             19194..19631
                     /locus_tag="KPG13_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984944.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            19643..20809
                     /locus_tag="KPG13_001208"
     CDS             19643..20809
                     /locus_tag="KPG13_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBG0984945.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            20824..21159
                     /locus_tag="KPG13_001209"
     CDS             20824..21159
                     /locus_tag="KPG13_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984946.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            21212..21784
                     /locus_tag="KPG13_001210"
     CDS             21212..21784
                     /locus_tag="KPG13_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBG0984947.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            21807..22379
                     /locus_tag="KPG13_001211"
     CDS             21807..22379
                     /locus_tag="KPG13_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBG0984948.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            22379..24337
                     /locus_tag="KPG13_001212"
     CDS             22379..24337
                     /locus_tag="KPG13_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBG0984949.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            24349..26766
                     /locus_tag="KPG13_001213"
     CDS             24349..26766
                     /locus_tag="KPG13_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984950.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            27048..27374
                     /locus_tag="KPG13_001214"
     CDS             27048..27374
                     /locus_tag="KPG13_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBG0984951.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            27323..28693
                     /locus_tag="KPG13_001215"
     CDS             27323..28693
                     /locus_tag="KPG13_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0984952.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            28717..28854
                     /locus_tag="KPG13_001216"
     CDS             28717..28854
                     /locus_tag="KPG13_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984953.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            28847..29326
                     /locus_tag="KPG13_001217"
     CDS             28847..29326
                     /locus_tag="KPG13_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBG0984954.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            29811..30047
                     /locus_tag="KPG13_001218"
     CDS             29811..30047
                     /locus_tag="KPG13_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984955.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
BASE COUNT        12066 a         3177 c         5363 g         9559 t
ORIGIN      
        1 agaagtatta ttatatttgg atttttggtt tttataattt ttttatattt aaacataact
       61 aaaaataaaa gattactaaa gctaagtaga gtttatttta ttttaataaa taataaagct
      121 atttttctat atattggcat aatatatgct tatttaacta tgaaattaaa attaaatagg
      181 attaatgagg aggaaaaaaa tgggaagtaa tgaactgaaa aagactcttg gcgtatcagc
      241 agcattatca actgttgttg gttccgttat aggagcgggc gtatttttta aaccacaagc
      301 agtctataca ttaactggag gagctccagg tcttggtata ttggcatggt taatagctgg
      361 aatcataact ataacagctg gtcttacagc tgcagaggta tcagttgcga taccaaaaac
      421 tggtggaatg atggtatata taaaagaaat ttatggagaa aaattaggtt ttttaactgg
      481 atggatgcaa attgtattat tttatccagg gatgatggca gcattaggtg ttatatttgg
      541 agaacaagca tcggcactaa ttggtagtcc atcactttta cttccaatag caattggaat
      601 tataataata gttgcagggc ttaatatgtt aggttcaaaa actggaggag tcatacaaac
      661 tgtttctaca atttgtaagt taataccact tattttaata atggttgttg gatttattaa
      721 aggtggtgga aataatccaa tacttacacc tatggttggt gaaggattaa gcctgggtag
      781 tgtactaggt caagttttaa ttgctatatt atttgctttt gatggatgga tgaatgttgg
      841 tactttagct ggagaaatga agaatcctgg aaaagacctt ccaaaagcta taataggtgg
      901 attgtcagtt gttatggctg tttattttat aataaattta gcgtatcttt gggtattacc
      961 agctaatgaa ttagcaaatt atgcttctcc tgcatcagca gttgctgaag ttatatttgg
     1021 ttcaatgggt ggaaaaataa tatctgtagg tatattaatt tcagtgtttg gagcattaaa
     1081 tggattttta ctaactggtt caagggtggc atatacttta gctacagata agactttacc
     1141 aaagtatggt atattttcta aattaaattc tgctcaagtt ccagcaaatg caatagcatt
     1201 agtatctgtt attgcatcta tatatgcttt gtcaggtcaa tttaatttat taactgactt
     1261 agctgtattt gctacttgga tattctatgt attaactttt ataggggtta tgaaacttag
     1321 aaagactcat ccaaatatac caagagagta taaagttcca ttatatccta ttgttcctat
     1381 aatagctata gcaagtggta tatttgttgt agttaatcaa ttgtgttttg caggtatgaa
     1441 aactactatg atatctatag gaggtcttgt aataacagct ataggcttac cagtgtatgc
     1501 ttatatgact agaggagtaa aaagataaac caagtagttt acaaatgctt cataattaaa
     1561 ataaaagaga ctgtctcaaa tagagataag tctctttttt attgttaaaa tggtaaagat
     1621 atgtttaatt ttacaaaaaa agagcgttcc atgaactaaa aagtttattt taaggtattc
     1681 attttatttt tagttttttg attataaaat taaacatcta aaaaattatt tattttaaaa
     1741 tactttattt taaagtgttt tttatcaaac taagtggtat ctgtacaaca aatattatac
     1801 atagctttta tgaaaattaa atctttctgt tatatataat agaatattgt attatataga
     1861 aaatattacc tgattaatat agtgatttat aaatgagatt agaaaatttt atttgataac
     1921 aaaaattaga taatatgaat atataatgta tgtattaaaa atgtggcaaa tagttgtttt
     1981 gctatttatt atttgtttaa tatttctaat ttataaaaaa aaaattataa attttaaaaa
     2041 ttataaatga cattttaact agaattaatt gtaaaaaaca ctatataata ctcaattttc
     2101 aacattctat acaattatac agaaattata cagcataata atcaccgaaa taacaaattt
     2161 taaaaattaa gcaaaacttt ttgtcgaagt tcaacgtcta tatatatgta gtgtaaattt
     2221 ttgggataaa atactacaaa aatataaatg taaaaatata gtaatattgg aggtcgaacg
     2281 atgagaaata aaaagaaaaa aataaataga aagaagttgt atttgctttt aagtgctatt
     2341 gtagtgtgtc tggcttttat agtagttggt gttcgcgtca gctctttaaa tatgaaggaa
     2401 aatgaaatta gtagaaatgc aaaactgtca agttctacta ttacagatat agtatctaat
     2461 acatcaatag aagttagtaa agggccaagt aaaagttctg gaaaaatagc atacataact
     2521 atagatgatg gtccatctaa atttactgac cagatgataa aaactttaaa taaatacaat
     2581 gttaaagcaa ctttttttat gatagatggg aatatgaagg aatatccaca gcaagttaaa
     2641 aatataatta aaaatgggaa cacagcaggt tttcatagtg tatcacatga tattcacaag
     2701 ctttatgtta ctagtacatc agcaaaagag gaatttgata caaatgatca aactttttat
     2761 aagataactg gtaaacattc aaaagtaata agaatacctt atggaagcaa accatataca
     2821 ccacaagcct cttatcaggc tttagttgat gctggatata aaatttggga ctgggactta
     2881 gatacagaag actggagatc taattcttct caaattgtac aaaatgtaaa aaatcatatt
     2941 aagaatagaa aaggtgaaga taaagaccaa cttgtagttt taatgcatga gaaaaaacag
     3001 agtgcagaag ctttagattc agttttaaaa ttcttatcag atgaaggtta tgaatttgcg
     3061 ccagtagatc aaaaccaaat accaaaaaat tattggttac gtaatttaga gtagttaaaa
     3121 tataagaata ttgattgata tttttagaaa ttgagaataa aaatgagtga ggtgtaggta
     3181 tattgaataa aaaactttat ataggaatgg tcggaatatt gtctttgatg atggttggtt
     3241 gtaataaaag tttagcaaaa gtaaatgatg tggaaatcac aaaagaacag tacaaaaaaa
     3301 caaaagcagt tttatctgct actaataatt atataaatgg gcagtcttta gatgaattag
     3361 aaaaaacttt ggataaaaaa ggtagaaata aattagaaaa tgttataata tcgtttatgg
     3421 tggataatga acttttatat caagaggcta aggataaagg actaactcca agtaaaagtg
     3481 aagtagactc aaaatatcaa gaactagaag ataagatgaa tttgaataca agctataagg
     3541 aaaaaatgga caaagcaggt gttgataaag attatttaaa acaagaaata tctagagatt
     3601 tagcaataga taaaaataaa aaggcctttg aagatagaat aaacataagt gataatgata
     3661 tggaagccta ctatacaagt cataaaaaag attttaatgt agaagaagta agtgcttctc
     3721 agatacttat atcgacttta gataaaaata aaaaagaagt aagcaaagat aaaaaagaag
     3781 ctttgaagaa gaaggcagat aatatattga ctaaaatcaa aaatggagaa agttttgaaa
     3841 gtttggctaa aaaatattcg gatgataaag caactggaaa aaatggagga cagctaggat
     3901 attttacaaa ggatgataaa aatgctgagt ttacaaagga agtgttcaaa ttaaagaaaa
     3961 atgaagtttc aaatgtgttt gaaactagtt atggatatca tatagtcaaa gttactgata
     4021 aaagagaaag acaaaaaagt tttaatgagt gtcagagttt aataagagaa tctattttaa
     4081 atgaaaagta tattgagcat ataaaaaaac tgaatgaaga tgctaaaata gacagataga
     4141 atttctaaca gctcaatcct agcctgtaat cggagggaga ttttacagga atgtttaaga
     4201 gtttaaagag aacgaaagtt gagaaataca ttatcgataa taaagactct ttttatagaa
     4261 tagcatatag ttatacgaag aatgaagaag atgccttaga tgtggttcaa gaagccatgt
     4321 ataaggcact atactcagtg gaaaatatta aagaagtaaa ttatataaag acatggtttt
     4381 ataaaatttt agttagaacg agtatagatt ttattagaaa aaaccgtaaa tataataata
     4441 tgacagatat tgaccttata gatgagactg gagagtatga taaatatact gatttggatt
     4501 taagaagagc tttggaagaa ttacctattg aatacaaatc tattataata ttaagatttt
     4561 ttgaagattt aaagattgaa gaagtagcaa taatattgga tgaaaatgtg aatactgtta
     4621 aaactaggtt atatactgct ttaaaaaaac taaaattaaa aattgaagag tagggataaa
     4681 aagaatgaca agcaaagaaa ggttaaataa gttaaaagat gagtatcata aaattccaat
     4741 acccaagaaa ttagatacta taataaatca tgaaaaaata gaaaacatat atcgagagaa
     4801 aaataaaaaa gtaaatagat taagatttaa agttgcaata gcctttgcat gtatatttac
     4861 agtattagta aatataagtc ctgtatttgc agacaatttt tctaagatac ctgtaattgg
     4921 agcaatagta gaagtaataa ctataaaaaa ttatagttta aaaagtgaaa attatgaagc
     4981 tgagattgat attcctaaaa tacgaggttt aaaagataaa aacttagaac aaagattaaa
     5041 cagtagtttt atggaagatg gaaaaaggct ttatcatcaa ttccaagaga gaatggagaa
     5101 gatacaatca tcaaagaata aaggatataa atctttaagt ttgagttata gtgtaaaaaa
     5161 taattctaaa aaatttctat caatagagat gacaaaaaat gagatagagg cttcatcata
     5221 tgtgagtaag gtacattata ctattgataa aaaaagacaa atagttttga ctttgccaat
     5281 gctttttaaa gatgataagt atataaaagt tataagtgat aatataaaag aacaaatgag
     5341 agaacaaatg aaaaaggata gtacaaaatc ctattttata gatcaaaaaa aagacttgcc
     5401 tgttgaagat tttaaaacaa ttaataagta tcaggatttt tattttaata aaaatgaaga
     5461 ccttgtaatt tgttttgatg aatatgaagt agcacctggt tatatgggcg cagttgagtt
     5521 tgtaatacca tataaagtaa taaaagattt gtaaattgaa tagtagttta tatataaatc
     5581 ttttaaggag gacaatatga ctacatataa aaaacttaaa aaatctatat ttaaaattgc
     5641 tatattttca atatgcttat tagcttcatt agtagataca aaccctatat ttgcaggtag
     5701 ttcctttaat gaaacttcaa atagtgatac gataaaggtg ttaaatgcta agagtatcaa
     5761 ctttaaacaa gacaattatg aagtgaatgt gagtattcct aaaataaaag gtttaaaaga
     5821 taaaacttta gagaataaat taaacaatga gtttttgcaa gatgggaaaa aaatttacag
     5881 tgaatttcaa aataaaatga aaaaggaaga taataagggt catagatacc taaatgtaat
     5941 ccataatgtc aaaaataatt ctaaggactt tttatcaata gaaatgacaa aagaagagat
     6001 agaagcttcc tcatctataa ctaagaagca ttacactgtt gataaaaata gaggaatact
     6061 attaaattta ccaatatttt ttaaagatag tagatatatt agagctataa gtgataatat
     6121 aaaagagcag atgagaaatc aaattaagaa agatgataca aaatcttatt ttatagacca
     6181 agataaagac tcgccagtga aagattttga atctataaat aagtatcaag atttttattt
     6241 taataaaaat ggaaatcttg tgattagttt tgatgaatat gaagtagctc ctggttatat
     6301 gggagcagta gaatttgtga taccaaataa agtaataaaa agactaagat agatatatag
     6361 aagattttta gatagactgg taaggagaat tatatgaata tatatgaaca agcaataaga
     6421 ttaatagaaa ataatgagga tttcgcattt gctacaataa cgtctcattc aggttcaaca
     6481 ccacgtgaaa ctggagcaat gatgattgtg aaaaatgatt ctactatatt tggctcagta
     6541 ggaggaggta gtgtagaagc agaatgtata aaacatgcaa ttaatgttat aaaaaacaga
     6601 gaatcaatgt tgtataaatt tactctaaac aaatctgatg ttgcaaaact aggaatgata
     6661 tgtggaggta ctggagaaat acaaatagat tttatagata gtaaattaaa aagtaatata
     6721 gaaaaattta ataagagact aaaggaaaat acaagtaaag catatatatt tggtgcaggt
     6781 catatttcaa gagatgttgc tgtgatacta tctcttttag aatttagaac agtagtaata
     6841 gatgatagag aagaatttgc taatcatgaa aggtttccag aatcagaagt agtagtttta
     6901 gattcttttg aaaacatacc agattttcca actgacgaaa atagttatat aattatttta
     6961 acaagaggtc atctatatga ttcaagtgct ttagaatggg ctcttaaaag agaggctggt
     7021 tatataggta tgattggaag tagaacaaaa attggattga cttacgaaaa actcatgaaa
     7081 aaaggtttta agaaggaaga attatcaaaa gtacatgcac caataggcat aaaattgaat
     7141 gctcaaacac cagctgaaat agctgtatgt atagctgctg aacttataaa ttgtagagct
     7201 aataaggaaa aataaactaa tgaacacagg gagtaaagat attgatactt taaaggttaa
     7261 ttttagaggg attgaaataa aagcagtttt agtatataaa aatagaaaaa atatttctat
     7321 aaaaatagat ccatttggca aaataactgt aatgagtcct cctaaaatat ccaaaaaaat
     7381 aataaaagat ataattattg aaaaaggaga ctggatactt aaaaagtcag aaaaatgtaa
     7441 aggaagagag gaagtatata aacaaagaat gtttataact ggcgaaaaat ttctttattt
     7501 aggtgaagag taccatttag ttataaaaga actgttgaga gattctgtca aaaaaagtaa
     7561 ctgtagaata actattaacg aatatcaaat agttgttcag acaaatgata caagtacaga
     7621 ttttattaaa aaatctttaa aatcttggta taagatggaa agtgaaagaa tagtattaga
     7681 gagaatagat tttcttaaat taaattgtga aataatgaag caattagttc cagcgagtgt
     7741 taaggtaaaa gaacaaaata agagatgggg aagttgtaca gctcaaaaaa atatttatat
     7801 aaactcaaaa atatctatgg caagattaga tgtaattgat tatgtaataa tacatgagtt
     7861 tagccattta gtacatatga atcactctaa aaaattctat gatttagtaa aatccattat
     7921 gcccgattat aaagataaag aaaattggct aaaaataaat ggatataaaa taataatctg
     7981 aaaacgatta ttccaaaata taggcgtaaa gtataaaaga agtattattg tgaaaaaaat
     8041 aaataatctg aaaattttaa aaaaagtcat tgacattgta aatgataggg aatataatct
     8101 atattaacaa caaaacagaa ttaaaatata aaattaaaaa attaaaagca atgacagaga
     8161 caacaatact aatagtgtta tccacagaga accaatgttg ctgagaattg gtgttaacag
     8221 tagtataatg atcactctgg agcttttgta ctgaatattt ttagtaagta caaacgtatg
     8281 tcagcgttac atgactaagg ttaaggtaat taattacccg acctgaaaaa agaggggtag
     8341 atgtattttt tatatctaca aataaggtgg taccgcggaa tataactttc gtccttataa
     8401 gtaatattta tattgcttat aaagatgaaa gtttttttat tttaagcgat ataaataaat
     8461 ttaatccata taaaattaat aaaaaaataa taaaaataag gagggatttt tatggaaaat
     8521 actgtatatg ctaaattaaa ccaaggaata gattcttttt taagagtggc tatgactcta
     8581 agaagaagag aggttgatat acaatcaata agtatgactg tagataatat gaataattca
     8641 ggtataaaat taatagtaaa tgaagaaaag acatccttag attctgtctt aaaccatatg
     8701 aaaaaactac atgatataag agagataact gttgaaagaa tgagacatta attaaatata
     8761 ttttataaag ctaaagataa atatgcttat tttaaaataa aaaactaaag ataaatatta
     8821 aataagaaaa atcttagatt aaaatataag tgtcattgaa tttattagtt tatcacagtt
     8881 aataaattta ttagtttgtt gtagctaata aaaactgaaa attctaaaat tataattgta
     8941 agattatata aatttagaat ttaaatctgt tgaataatat taaaacaatg ataatgagta
     9001 tgaatttcta ataaaaataa atatcataaa aataagaaaa agtattggga ggaaatgaaa
     9061 atggcaagaa tgtattatga aaaagatgtg gatttagaag ttttaaaaaa taaaaaggtt
     9121 gcagttttag gttatggaag tcaaggacat gcacatgcac aaaatcttag agataacggt
     9181 gtacatgtag tgataggtct ttatgacgga agtaaatcag cacaaaaagc aaaagaagat
     9241 ggatttgaag ttaagagtgt agcagaagct acaaaagaaa gtgatttgac aatgatgtta
     9301 atgcctgatg aaaaacaaaa gaaagtttat gaagagagtg taaaggataa cttaaaagaa
     9361 ggccagacat tagcttttgc tcatggattc aatatacatt ataatcaagt acaaccacca
     9421 gaatttgttg atgttgtaat ggtagctcct aaaggacctg gtcacttagt tagaaatgta
     9481 tttacaaagg gaagtggagt tcctgcacta tttgcagtat atcaagacta cactaaaaaa
     9541 gctacagaga cagttttagc atatgcaaaa gggataggag caacaagagc aggtgtttta
     9601 gaaactactt ttaaagaaga aacagaaaca gatttatttg gagaacaatc agtactttgt
     9661 ggtggaataa gtgaacttat aaaattggga tataaaacgc ttgtagatgc aggttatcaa
     9721 aaagaagtag catattttga atgtttacat gaaatgaaac taatagtcga ccttatatat
     9781 gaaggtggat ttgaaagaat gagatatagt ataagtgaca cagcagaata tggagattat
     9841 gtatctggta aaagggtaat aactgatgtt gcaaaacaag gtatgcaaaa tgttttagag
     9901 gatatacaaa atggaaaatt tgcaaaagcg tggattaaag aaaatgaaga aggacgtgaa
     9961 aacttcctaa aaactagaga agaagaatat aatacagaaa ttgccgaagt tggtagaaat
    10021 ctaagaagta tgatgtcatt cttaaaataa atatatatta aatggattag tttgagggaa
    10081 agagtggtgt atagtagtgc gtatgaatgg agcaaaggta attttagaat gtttaaagaa
    10141 agagggaatt gacacaatat ttggatatcc tggtggagcc gtgatacctc tttatgatgc
    10201 tctatacgat tattcagatg attttaaaca tataaggaca tcccatgaac aaggtttagt
    10261 tcatgctgca gatgggtacg caagaagtac taatacagta ggagtttgtt ttacaacatc
    10321 tggtccagga gcaacaaatg ccataactgg aatagcaaca gcttttatgg attcttcacc
    10381 tatggttgta atttcagggc aggtgccaac tagtttatta ggaaaagatt cttttcaaga
    10441 aattgacata actggagcaa cattatctat gacaaaacat aattatttag ttagaaacac
    10501 taaagaatta gttcctacaa taaaagaagc ttttagagtt gcaaattcag gtagaaaagg
    10561 acctgtactt gtagatgttc caaaagattt atttttagca gaaatggatt ttagtggtga
    10621 ggactatgat ttatgtcaaa tagatgatta tatggattat aagagtgatt ttgatctaga
    10681 tgatgaaact aatattaagc ttttaaatga agcaatagat atcataaaag aatctaaaaa
    10741 gcctataata tatgcaggtg gaggtgttaa atcttcagac agtgaggaaa ttcttgaaaa
    10801 atttgcaact aagatagata cacccgtact aaatacactt atggggcttg gaaatataga
    10861 tagaaagaat gaattatctc ttggaatggt tggaatgcat ggaagtagag aaagtaattt
    10921 agcactttct aattcagatt tagtaatagc gataggtgcg agatttagtg atagagtgat
    10981 aagtaaaagt tctgaatttg ctaaaaatgc aaaaataata cacatagaca tagacccatc
    11041 tgaaataagt aaaaatatag aatctaatgt atctttggtt ggagatgtaa agctagtcct
    11101 tagtttatta attgaaaggg ttgaaagtaa gaataatagc aattggaaag aagagataaa
    11161 gagatttaga aagagtgagg gtgtacaaac atatgaattt catccacaaa atatacttaa
    11221 aaaaataaat gaaaaatatg aaacattaaa gaaacccact gtggtagtaa ctgatgtcgg
    11281 tcaacatcaa atgtgggctg ctaagtactg gaattttaaa ggtaataaaa gttttataac
    11341 atcagcaggg ttaggaacta tgggatttgg tttaggtgca gctattggaa ctaaagttgg
    11401 taatgtagat aaaaatgtgg ttttagtcac tggtgatgga agttttagaa tgaactgtaa
    11461 tgaactagct acagttgcaa attataatgt acctatgctt attttactac tcaataatag
    11521 aacattagga atggtaaggc aatggcaaaa gttattttcc aatcaaagat actctcaaac
    11581 tgacattaat gaaaatgtgg attatgttaa actcgtaaat gcatataaca ttgatggata
    11641 taaggtatcc agtatggaag aattaggaaa ggcgctagat atgatagatt ttaataaacc
    11701 tgtatttttg caatgtgata tagataagga ttatgatgtt tatcctattg tagcacctaa
    11761 tgatgcattg gaaaatttga tatgtaacta aaacttatac tgaatattga taaaattttt
    11821 tattaaattt ttattagtat tcagtttttg ttgtttagag atagattggc attttttatt
    11881 tttgcaaata aatcaatgac tatataatta agaacttatt aaattttaat tatacaattt
    11941 agaatagtat tacaacttaa gtaaatatta agttttattc attagattta ttattaatac
    12001 cttatattag ttatggttgt tatatactct aaagccttga aattacatgc gtatagctga
    12061 ctatataaat ttgataattt tagcttttaa aatagataaa ttagattttg ccacccattt
    12121 gccaccgtat tatatttttg ggtggcaaac tctaatttgt aattttttca aatatatcta
    12181 cagtttcatt tttcatttta tcagttacat gtgaataggt atccattgta gttgatagtt
    12241 ggctatgacc caaacggttt tgtatgtctt taatgttagc accattttct aataatagag
    12301 tagcatgtgc atgtcttaaa gaatgaaaat ggaagtcatt gtttaaagct actcgaattt
    12361 gtcttactat agtgtctaaa gtgtgagtat tcacttgttg accattttct ttggtacata
    12421 cccagtcact atcaaagtaa aattctccat attttaattt cattttcttt tgatataatt
    12481 tatgttcttt taatgccttt attaaagtgt cacctgtaaa tatagttctg caagagcttt
    12541 ctgtttttgg ttgccctaat tcaaacattc catttggctt tttaatcaga gtatgtctta
    12601 ctgtgatagt tttattatta aggtctatat tatcccattt tagtgcaata atttcacctc
    12661 ttcgcatacc agtatgaaat ccaattagta aaactatacg ttgaaatgaa tcttgaggaa
    12721 atatatttag tatttgatta aattcttcta ctgtaatagt tttaacttta ttagtttctg
    12781 ttttagattt agtttttggt atgcttacat attgcatagg gttttctcgt atgtgtttgt
    12841 aaggatggac tgctgatttt aatgacctat gtaatatggc ttttaatact tgtaatgtat
    12901 tttgagagta atcctctttg tactttttat ttatgaagtt ttgtagtatt gcaggagtta
    12961 aagcttttac tttgtaagct cctagctttg gctttatatg tttttctatg tttattcggt
    13021 agctttcttg agtgttgtat ttacagttaa gtaagacata ttctttgtac caaaagtcta
    13081 agtagtctga taaactgata ttgctttctt caaatactat gccagaattt tcatattcat
    13141 ttagtgcttc tcttaaggct ttttcggctt cttttttagt attgcctcca actctttcta
    13201 ctttttttct ttttccttct actatgccta ggtcaaagta gtaataccat ttgttacttc
    13261 tttttcttac tccacctttc ataatagtat ccctcccttt tagaatgtat gtttgtttgg
    13321 tgtttatata aaagagcaga ttaactgctc tttatatact tttgttatgt acaatattat
    13381 cttaattttg tttatctggt ttttacttaa ttattttctt aatattctta caatttttta
    13441 gcttttatgt gtagtttatt ataataatat tgaaatatat atttattaag taaaaaatct
    13501 ataaaagtag aataaatgag tttaaaaaat atcctcatat atgtagaaat ttggcaaatt
    13561 agttaatact aaaaatctat tatttcctaa gtccaacatg cttttctttt ttgatagaaa
    13621 ttctaatctt tttagtaaga agttgatgct aacttgtaat tcttcagcta tttcgtatac
    13681 acttgtagcg tgtgaattaa taacatgtat tatttcttct tctgttataa gaaattcaca
    13741 tgcccatttt aatgctttgt tttcagtttt gtctatcaag attttatttt tgtaactgtt
    13801 ttttgaagat acatagttcc caacactggt aaaatgatgt cctaattctt ctgctaagat
    13861 ttctattagt ttagcattgt tttgttttaa tgaattaagt aatgatataa tctttagtcc
    13921 ttgtctgttt atacaatcct tttatgtcat ctgctatttt gtcagtgtag taaatttcta
    13981 tctcttcatt atttgctaag tctaaaagtg cgtctagttt gttcattgaa atccccctat
    14041 aaaaagaatg tatttttgat ttttgttata tataaagagc aggtgtgaac tgctctaaat
    14101 attcattttt tatccaaaaa agattggata attttagctg tagacaaaat atggattata
    14161 gtatatgatt ataatataat tacatataat atatgtaagc acttacaaat gtgtgttagt
    14221 atagttagca ctaaatttgt atttgtgctt aaagcgatta aaggagtttt ggagcggtgg
    14281 gtagagtttt actcactata accaagactc ttttgcatat tagagtattt catgtagtca
    14341 tatattctaa taagccttct taaatttgtt ttttcgcttg tttttgcaga ctctaaaata
    14401 ttaggatttt cctcaaatat tttcttaagt agattttcta actgttcagt gtcaatttct
    14461 ttttcttcta aaaaagtaat aagtcttgta gctttttcaa atgattgatg ttcatcattc
    14521 caaataccat aaacacttct attttttatg taagtattat ttctattttt tttaatacta
    14581 ttgctaataa tattttcaaa gtcactttct agtgcaattg tcctataatc tggaaattca
    14641 agtgtgctat taaaaagatg tttatttaat ggtaatttat tatattgctt aattaaagat
    14701 ggataagcat attcaagtaa ttcatctgat gaaaattcta agtcattcat gactatatca
    14761 cgataccatt catcaaaact taatcctttt aatcctttaa gaccaaaatc acttgccttg
    14821 ccaactgcta aaactaaatc ttcatctttt attcttgtat catcaacact accaacccga
    14881 attttagcag taggtttgtt agtaattgtg aaatcgtaaa attcttgttt gaacattctt
    14941 aatattctaa ctggtaattt tgattttttt tcagataatg cctcatagag taaattaaaa
    15001 tcctccagtt taattttagt catagtaatc attttattat caaaagcaat tgtatgactt
    15061 gatatttcta tatcattaaa gtttttttca tactcaatga atatgaaacg ttcctcgagt
    15121 tttttagcat tttcattaga tagacaatta actatagcat taagtatttt ttttatattt
    15181 acatctgtaa cagagtaacc aataaatatg ataggaaact caacaaatat agtcattagc
    15241 ttagctgcca agtaggcact tttgctgtca aagtctatat aatcagcttc atttattaca
    15301 atactatttg gagaagaaac acatccatga attttataaa tttctgcaat accttgtata
    15361 gatgaaaaaa ctaaattttc ttgacctata tatgttgtat aattatcaac gatagtttcc
    15421 aaaaaacaat cataatttgt tgtaataaat cctgataaac tttttttaga gatattacta
    15481 agtttttcaa cttcatcttg atatttatct actattttag aattattttt aatatacata
    15541 gcaatttctg ccttaaatgg agaaactcct gatttaacaa tatctaaata cttagagtct
    15601 aagcttcgta tagaagaatc tgaaaaccat tttttattaa aatcattttc aataagttct
    15661 gcaattctag gatttatacc catttttggg ttctctgatt ttgctttatt tttataactt
    15721 gtatatataa agtcatcatt actaattttt tctgcaaaga cttttagtaa atcctcccaa
    15781 tttggaagat tatagtatct tctagttaaa ccagaaccaa caaataaaaa tggtgtagca
    15841 ttaaatcggt ttatgacatc tgatattata gatttcatat aatttatcac ctacttttaa
    15901 tttatttttt atacttattt attaaaaatt caatataatc attgagttgt tcttgagctt
    15961 cctctggtaa atctttgtga ggattaactc tatgtgcagc tacagtgtct atatcatttt
    16021 ttacaataga cctacctaat aaataatcag tagtcacatt aaatatttca gcaaggctag
    16081 ataacatata agcatcagga actgtctttc cttgttcata ataaccataa gcgcttttgg
    16141 ttaagtttaa tttatttgca acatcttctt gagtcatatt tttttcagct ctaagttctt
    16201 ttagtctttt ttgaaataat gtattattac aagaagtttt gctagaaata ttgcttgctt
    16261 tattatcata cctacctatt aaataatcaa cagtagtgcc aaaatattca gctaaactag
    16321 ataacatttc tggggtaggt gttcttttac cttgttcata aaatccatat gcactagtag
    16381 ttatgttaag atagttggca atttcttttt gcattacacc tttttctttc ctaagaaatt
    16441 tcaatctatc tgataaaatg ctcatataat cactcctctt aataaagttg cctttattaa
    16501 ataatacaac aaatagttgt aaaatgaaat aaaaaaataa aaaaactatt tacaaacaac
    16561 atatagttgt gtattatata agtataaaca acaaaatgtt ggtaaggagg atatagcttg
    16621 aacaatctaa aggaacttag gacagaaagg aatttaaagc aaaaagatgt tgcattaaaa
    16681 gtaggtataa ctactagtta ttatggaatg attgaaagtg gaacaagagt accaagttta
    16741 gcaatagcaa taaaattatc taaatgtttt ggagtatcaa tagaaaaaat ttttttaaat
    16801 acaaatacaa caagatgttg tttaaaagag aggagttaaa tatgaaagat ttaaaaatag
    16861 taaaagttaa taataaacta acaactgaca gtagagatat agctctaatg gttgaaaaag
    16921 aacacaagat tttactaagg gatataagaa attatataaa ccaaatggaa gaagccaata
    16981 aaaacatgag tacagatttg tacccatctg attattttat tgaaaatact tatttagatg
    17041 attataaaag agagaagcca tgctatgcaa taacaaagat aggttgtgac ttcatagcaa
    17101 ataaaatgac aggcataaaa ggtacggcat ttacaggaat atatacaaaa aaatttgaag
    17161 aaatggagca agtattaaaa aatgaacaac ctaaactacc aactacatat aaagaagcat
    17221 tacaacatct tatagagcaa gtagaagtaa atgagaaatt acaactagaa ggaaaaatga
    17281 aagaccaagt aataaaagaa ctaaaaccaa aggcagatta tacagatatg atacttaaaa
    17341 acaaaggtct agtcactata actcaaatag caaaggatta tggaatgagt ggaaaagaaa
    17401 tgaacaaaat acttcatgaa agagggattc aatataaaca aagtggacaa tggcttttat
    17461 ataaacaaca tcaagggaag ggatacactc attcagaaac aatagacata actagaagta
    17521 atggaatgcc tgatgtaaaa atgacaacta agtggactca aaaaggtaga ttgtttttat
    17581 atgatttatt gaaaacaaat aacatattac cagatataga aaaagaatat agttatcaaa
    17641 cttcaatatt aggttagtac tttgaaaact aaatacagaa tattttggaa aggagagatt
    17701 gaattggaaa ataagaaaat agaagaaata gagaaaagaa taaccatgct tgaaaatcaa
    17761 ttgcaagcac agttatttag tcagataaca gctcaatcaa atagtgattt tatagattat
    17821 atgtaccaat gtatagtttt gtatttacat gagatgagta aaagtgaaaa aagaaaaatt
    17881 agtgaaatta ctaaagaaag aatcagaaaa taagaatgat aattacaata taaaacctat
    17941 tcttctagca ggttttgaag ggtcagtttt ctttactgaa gttatcataa agtttatctg
    18001 tgatgtgtgt tgaagaattt cagaataaac tccatttact atgccgtagt agtgaattag
    18061 acaagggtta tgataaccta cttcttcaac aatcattaat acatcttttc caaagttagt
    18121 caattttagt gcaatttcat gttcatcatc aagttctttt tcaaattcct ctatagattt
    18181 aagaataatt ttatattgaa attcagcata gtaaaaatac tctgtgttag aagtatcaga
    18241 gactgtcttt cttatacttt cttgtaattc tttagatata ttgtttgaaa actgcataat
    18301 acaccatcct tttgaaaatt ttggaattta ttccatgttt atattatacc atgtagaact
    18361 gaggtgaata caatgttaat aggcgacaat ataaagcaaa tactaagaaa aagagatata
    18421 aaaccttata aattggcaaa agaattggat atagatgtaa gtggtttata taaattactg
    18481 agaaataaaa gttctaatcc aactatagat actctaataa aattagctga ttatttagat
    18541 attacattag acgaattggt tggaagataa ataattgaaa agaggggaga gcattttcac
    18601 atggatattt ctgaaagcat aacaaaacaa tttagtgata gcttaaaaag cttaatagaa
    18661 atagagataa gtaaacaaga gacagataga gttaagagtc aaactattga acaaaaggta
    18721 aaggtactgg agcctaaaga tatagttgtt ttaataaaaa gaggttatcc aaattatcta
    18781 ataacagtag aagaagcaag gggaatttta aagttagata cagcttttat gcgtaggtta
    18841 gtaagcacag gtttgataaa atcactagtt agaggtgatg gtagaaaaat ttcaagatat
    18901 gaagttgatg attttattga aagaaatcaa ggtaaaaatt tggatgaact tttaaaagca
    18961 gtagagaggg gggatgaaat tgctgagtct tgatactaat aataagaatg tagtaactct
    19021 taaaaaagat ggaaaagtta tagcagatat agtatttaaa gatattaaaa ctggtaagaa
    19081 aatatcggtt ggaatattaa ataaaaaagt gttggtaaaa taaccaacac acaaaaaaat
    19141 aaaaaataaa atataacaca agtaaagtat aacacaaaaa ggagagattt aaaatggttg
    19201 aagtgaatgt aaatgtaaaa gttaaagtag aagcaccaga atttacaaat gcactattag
    19261 tagtggcgaa tgctttagga ggtcttaatc taggaaaagc aatgcagata gaacctataa
    19321 atataacaga tatgaaagaa gagaagaaag ttgaagttaa aaaagctgaa aagattaaaa
    19381 aggtagaagt aaaagaagac gttaaagaag aaatagcaga agcaaaggaa gaaatagaaa
    19441 agaataatga aaatacaact agtgaagtta aatacacaaa agaagaggta agaactaagg
    19501 cagcacaagt aagtaaggca ggtaagaagg ataagcttaa ggagttattt ggtgaatttg
    19561 gagctagtaa gttaagtgaa gtaaaagaag aagattattc agcttttatg aacaaattag
    19621 aaagtttata gggggtacat aaatgccatt acaacatgca agacttagtg cgagtggagc
    19681 ccatagatgg cttcactgca ctcctagtat aaaattagaa gaaaactatc caccatcaac
    19741 tagtatatat gcagaagaag gaacagttgc acatgaatta gcagaagtta aattaatgct
    19801 agagtatgaa aaaataagta aaaaggcata taatgcaaga attaaaaaga tacagaaaag
    19861 tgagtattat aactctgaaa tggaggacta tatacaatct tatgttgaga atgtagttga
    19921 attagtaaat gatagtaaag ctatatgtga tgatgtaata gtgatgttag aagaaagact
    19981 cgattttagt gagtgggttc cagaaggatt tggaacagga gacgttgttg taatatctga
    20041 tggtatactt caagttatag acctaaagta tggaaaaggt ttagaagttt cagctataga
    20101 gaatcctcaa cttagactat atggtttagg ggcatataat cagtttgaaa tgctatacga
    20161 tattgattta attaaaacaa caataattca acctagactt gataacatat caagtgaaga
    20221 aatagaagtt actaaattac ttacatgggc agataatgtt aagaagaagg ctcaaatggc
    20281 ttttaatggt gaaggagagt ttgtaagtgg cagtcattgt ggattttgta gagctaaaaa
    20341 tgattgcaga aaaagagctg aggataacct taaactagct agaaaatatg attttgctga
    20401 cacatttgct cttaataaat atgagatagc agatatttta ggttttgcta agaatataca
    20461 agattggtta aaagatgttc aaagttatgc actagaacaa gctgaaaagc atggtgttaa
    20521 atatccagga tataagcttg tagaaggcag aagtaataga aagtatgtag atgagcaaga
    20581 agttgctaag gttttattaa attcagatta tgatgaagaa aaaatttata agccaaggac
    20641 cttaaaagga attagtgata tggagaaagc tataggtaaa aagagttttg ctaaactact
    20701 aagtgattta attataaaac cagttggaaa agctacttta gtagtagaaa gtgataaaag
    20761 gtcagaaatt aatagtatag attcagcgaa aaaagatttt gaaatataaa tgggggatat
    20821 tacatgccat tagatgcagg tataaaagat aaattagatg aaagcataag tataatttta
    20881 gatttaaagg atagaaaaaa gagtatcgaa aaagatttag attatgaaaa agataattta
    20941 ctagatatgt taaagatact aaatattaca gaatataact caactgaaga aaatgcaaaa
    21001 gttatagtga tggattttaa aagagaaagt ttaataaagg ataaagtgat gtcaactttc
    21061 ctcgaagtaa atgacaatca tcttgataga gtgtatatac cagaacatat aaaagttagt
    21121 ccagtatgct ttgtatcagt aagagcaaag gataattaaa aatatttaat tcctagtagc
    21181 tataaaaaga tttaaaaggg gatataattt tatgagtaat caagaaataa ctaaactagt
    21241 tgaaaataat ttgaatcttg tacattttag tataaataga ttttttaagt cttatgtaga
    21301 gcaacatcca tacttatatg aagagtttta tcaagaagga tgtattgggt tatataaagc
    21361 agcattaaat tatgatagta acagaggcaa gttttctaca gtggctgttt cttacataag
    21421 aatagaaatg tttaaagctt tgcgttatgg cgaaaaacat tatagaaact ctatagaagg
    21481 tttagatgta agtgtatttg aagatagtaa aagaccatta aaagatatac atggatttta
    21541 tgaaattcaa tttaactatg atgttgatta tataagaaaa attgcaagaa agtcatattt
    21601 aaaagatatt gataaaatag tagattggat tttacaagat aaaaagatat ctgatatagg
    21661 agagttatta aatgtttctt caacagttat ttgtggaaga attaaattgt ttagtaagca
    21721 agttagagat ttagataaat ttggagaaat aacttcaaat ataaaatcaa tataccaaat
    21781 ataaaaatat taggaggaag aaaattatga gtaattcagt acaatcaaca aaggtagtaa
    21841 caggaaaggt aagattaagt tattgcaata tctttaaaag cagagcaatg gtagaaggtg
    21901 cagagcccaa gtattcagtt tgtattttaa taccaaaatc agataaggtg actttaggaa
    21961 gaataaagaa ggctattgat gcagctaaag aacaaggtaa aacttctaaa tggggtggaa
    22021 aattaccagg taatttaaaa acacctcttc gtgatggaga tgcagaaaga gctgatgaag
    22081 cagaggaata tgtaggaatg tattttttaa atgcaaatag tactcaaaag ccaggaatag
    22141 ttgataaaga cttaaatgaa atattagata atacagaggt atatagtggt tgttatggaa
    22201 gagttagtat aaactttttc ccatataata gtgcaggaaa taaaggaata ggttgtggct
    22261 tacaaaatgt tcaaaagtta gcagatggag aagtacttgg aggagctaga gctagtgcag
    22321 aagcagattt ctcagatgac tttgaatatg aagatgaaga agaggacttc ttaagttaat
    22381 gaggacctta tcaattgata tagaaacata tagtgattta gatataaaaa aagttggagt
    22441 ctacagatat gtagactctg ctaattttga gatactgtta tttgcctatg cttttgataa
    22501 tgaagaggta aaagttattg atttagtaaa tgatgaagag ttaccaaaag aagtaataga
    22561 agctttaaat gataataaag ttataaaatc agcatttaat gctaattttg aaagaacatc
    22621 aataagtaaa tttttaaata ttaatttaaa accaaatgag tggtcatgta caatgataaa
    22681 ggcgttaaca ctaggacttc caggaagttt agatagtgtg tctaaggctt taaagtttaa
    22741 tgaagataaa cagaaaatga aagaaggtaa agcattaata caatatttct gtaaaccttg
    22801 taaggctaca aaagttaaca agggaagaac tagaaattta ccaatacatg atatggaaaa
    22861 atggaataaa tttaaagaat attgtaaaca agatgttgta gttgaaagag aaataagaaa
    22921 caaacttagt aagtataaga ctaccgaaag agaaattaaa ttatggtatt tagaccagag
    22981 aattaatgat actggtatta aagtggatac agagttaata gagaatgcaa tagaatgtga
    23041 taaaagatat actgaaaaac ttacaaaaga agcaattaaa ataactggtc taaataatcc
    23101 aaatagtcca gctcaattaa aaaaatggtt aagtgataaa gttggctttg agattacaag
    23161 cctaacaaaa gaaagtattc cagaaatatt aaaacaagtt gatgatgaaa atgtagttag
    23221 aattttagaa cttagaaaat taatgtccaa aacttctata aagaaatatg aggctatgaa
    23281 attagctaaa ggtaatgaca atagagtaag aggtctacta cagttttatg gggctaatag
    23341 gactggtaga tgggcaggaa gattagtaca agtacagaat ttaccacaaa atcatataga
    23401 ggatttagac ctagctagaa acctattaaa agaaggagat tttgatttaa tagagctttt
    23461 atatgatagt gtaccagatg tcttaagtca attgataagg acagctttta taccaagtga
    23521 aggtcataga tttatagtgt cagattttag tgcaatagaa gctagagtta tagcttggct
    23581 tgcaggtgag aagtggagac tggatgtgtt taattctcat ggaaaaatat atgaagctag
    23641 tgccagtcag atgtttaaaa ttccaatcga aaatattaaa aaaggttctg aacttagaca
    23701 aaaaggaaag ctagcagaat tatcgaatgg atatggcgga agtgtagggg ccttaatttc
    23761 tatgggggct attaaaatgg ggcttaaaga agaagagctt caacctattg ttactgcatg
    23821 gagaaatgct aatccaaata taactaagtt ttggtgggat gtagataagg cagctaaaaa
    23881 agctataaaa gataggacta tagtagaaat tcaacatggg attaaattta tttataatcc
    23941 aggcgtttta tttatagaac taccaagtag tagaagatta tcgtatctaa ggcctaagat
    24001 agagccacat actacattta gtggggataa gataacatat gaaggtatgg aacagacaag
    24061 taaacaatgg aaaagaatag atacttatgg acctaagtta gttgagaata ttgttcaagc
    24121 tacagctaga gattgcttaa gagaggctat gtttaatgtg acagatgcag gttatagcat
    24181 tgtaatgcat gtacatgatg agcttgtaat tgatgtagat aaaaaaggag gttctttaga
    24241 agaagttaac agtattatgg gaaaagaaat atcttgggct aaaggtcttc ctcttaaagc
    24301 agatggatat gaatgtgatt attataagaa agactaggtg attgatttat ggatataaag
    24361 gccagtgaaa ttgaacacat aaatgtaaga catgatggcc aactcatgct ggccatagga
    24421 aaaaataaat tagaaacaca ttggaaaaat aaaagtattt tatggtccga acttgtaaat
    24481 agattaagta aaacattaag aactcaagaa acatatacag aatataggaa gatgtcaaag
    24541 actgaaaaag atagagttaa agatgttggt ggatttgtag gtggaagctt aaaaaatgga
    24601 agacgaaaag cagagaatat tgcaaataga agtattataa ctttagatat agattatgcg
    24661 aataaagata tatgggagga tataacatta ttaaatgatt atgcttgtct tatgtattct
    24721 acgcactcac atactgagaa taatcccaga tatagacttg taataccttt aactagacca
    24781 gtactaccag aagaatatca agcaatttcc aggatgatag cagatactat aggaatagat
    24841 atgtttgatg atactacata tcaacctcat agacttatgt attttccaag tacttcaatt
    24901 gatggagact atatatttaa atttcaagat ggagagtttt taaacccaaa tgaaatacta
    24961 gatttatatt tagactggac agatgtaagc tattggccag aaagttcgag ggagaggcaa
    25021 aagtttaata cacaattaaa aaaacaacaa gaccctattg aaaaggctgg aattataggt
    25081 gcattttgca ggtcctatag tataaaagaa actatagaaa ctttcttaaa tgaagtatat
    25141 attcctggta ttgatgaaac caggtataca tattcagaag gtagtacaag tggtggagta
    25201 gttatttatg atgacaagtt ttcatatagt catcatggta cagacccagc aagcggaatt
    25261 ttatgcaatg cttttgattt agttaggata cataaatttg gtgaacttga tgaagatgct
    25321 aaacccgaaa cgcctgtaaa tagattacct tcatttactc gaatgagcga atttgcaagc
    25381 agtgacacta aagtacgaaa gactatagga agagaaaacc ttgataaagc taaggatgat
    25441 tttggtgata tagattttga agatgatgaa tggttaacta ggttagatta tgacaataag
    25501 ggaagttata agaaaacaac aaacaatatc ttaatgttta tagaaaatga cccatatttg
    25561 aaaggaaaaa tagcttataa tgaattttca aatagagctg tcgttttagg taagttacct
    25621 tggagaaaag atgataaatt aaatgattgg aatgatagtg atgattctgg gcttagacat
    25681 catatagaaa caatttacaa tatctcatca ccatcaaaag taaatgatgc tctaataatt
    25741 gcttttgaaa ataatacttt tcatcctata aaagattatt taaattcttt aaagtgggat
    25801 ggcattaaga gagtggatac acttttaatt gactatttag gtgcagaaga taaccactat
    25861 acaaggacta taataagaaa agttttagta gcagcagtag caagagtatt taatccagga
    25921 ataaagtttg ataatatgat ggttttatct ggtcctcaag gaatgggaaa aagtactttt
    25981 attaaaaaac ttggtggaga ctggtattct gatagtttaa ctactgtaca aggtaaagaa
    26041 gcgtatgaac aattacaagg agtatggttg ctggaaatgg gtgaaatgat ggctactaaa
    26101 aaagcagata ttgaggcagt taagcatttt ctaagcaaat cagaggatat atatagggtc
    26161 gcatatggga agagaacctc aagattttta cgtcaatgtg tagttatagg aacaactaat
    26221 gataaagaat ttttaagaga taagactggg aatagaaggt tttggccaat agatgcagga
    26281 gtaaagaaga ctaacaagag tatatttaat ggccaacttg ataatgaaag aaatcaaatt
    26341 tgggcagaag cagtagaatt atataaagcc aatgaacagc tatatctatc agatgaggag
    26401 aaaaaagaag ctgaaagaca acaaagaact cactcagaag aaaatgctaa atctggaatt
    26461 atcgaagagt atctaaataa acctattact aagaattggt atgatttaag tatttcagaa
    26521 aagagagaat atattcatgg ttcagatttt ggtgatttaa aagaagggac aatattaaga
    26581 gaaaaaacgt gtgttatgga gatatgggtt gagctattta atggagaacc taaacaactc
    26641 acgcctatct tatcaagaga aattaatgat atattaaaag gattagatga ttggatatca
    26701 tataatggaa atttaaggtt tgggaaaata tatggtaggc agagagctta tgtacgtaaa
    26761 agttagtgtc aacagaatta aaaatagagt aaatttgaaa attttaaaag tgtcaacaat
    26821 gtcaacaaaa aaaatggctt tgttgacgct tatgttgaca cctaagatgc agtaattaca
    26881 atgtttgagg tattaatgtc aacaatgtca acaaaaatta agtataaagt agtataagta
    26941 atattaggca tatacgtata tacatatgta tgcctaatat gtatatatac atatatatag
    27001 aaaatctgtt gacattgttg acacttaaat tttaaggaag tgatattttg ttagaatcaa
    27061 aaatagaaaa aagacttaaa aaagagattg agttgttagg tggaaaggct atgaagttta
    27121 tttcaccagg tgaggcaggt gtgccagata ggattgtctt attaccagaa ggacatgtta
    27181 tatttgtaga acttaaagca ccaggtaaaa aactaagaaa gcttcaacaa tataaaatgc
    27241 gagaattaag agaattagga tttaaagtta agtgtgtaag cacattaaaa gagatagatg
    27301 attttatcaa ggaggttaag ggatggaatt taaaccacat ccataccaag aatacacaat
    27361 tagaaaaact atagataata ataatatagg tttactatta gatatgggat taggtaaaac
    27421 agtctgtgct ctaacagcta taagtgaatt aatgtatgat tactttgata tatcaaaagt
    27481 tttagttata gcacctttaa gagttgcaag agatacctgg agtagtgaag taaaaaaatg
    27541 ggagcatcta aagcatctaa aagtatctaa agttcttggt agtaaattag atagagtgag
    27601 agctttaagt acagactcag atatttatat aataaataga gaaatggtac cctggatagt
    27661 ggatttttat aaaagaaaat ggccatttga tatggttgtg atagatgaac ttagttcttt
    27721 taaatcaaat aaagcacaac gatttaaaag tttaaagaaa gtattgcctt taactaaaag
    27781 agtagttggg cttactggta caccaacacc aaatagttta atagacttat gggcacagat
    27841 gtacttactt gatagaggtg aaagactagg taaaactatt acaggatata aagaacgata
    27901 ctttgagcca ggacaaaaaa attatcaaac aggagctata tataattggc agcctaaaga
    27961 tggagcagaa aatgcaatac ataataaaat aaaagatatt tgtataagct taaaagcaga
    28021 agattatttg aatatgccta aaaaaataga taataaaatt gaaatacacc ttgatagtaa
    28081 aatacttaaa tattacaaag aattagaaaa agagaagata ttagaattag aaaaagatat
    28141 aataacagct tcatcagcag ctgtagcagc aaataaactt ttacaattag ctaatggagc
    28201 aatttatgat aatgataaaa atgtaaagga actccataga gaaaaattag aagctttaaa
    28261 agaaattata gatgtttcaa atggtaagcc tattatagtt ttttataact ataaacatga
    28321 ttataatcgt ttaatgaaag agtttaagag tttgaaacca agaacaatag aaaactcaaa
    28381 agatatatat gattggaata atggaagaat acaattatta ctttgtcatc cagcaagtac
    28441 aggacatgga cttaatttac aatcaggtgg aagtataatt gtttggtttg gattaacttg
    28501 gagcttagaa ctgtatcaac aagccaatgc aaggctttat agacaggggc aaagggaaac
    28561 tgttattatc catcatctaa tctgtaaagg tacaatagat gaacaagtaa tggaggctct
    28621 agagaataaa gataaaggac aaagtgcatt acttgaagca gtcaaagcaa aattaaaaga
    28681 atatagggag tgaaggtatg aaagtaaaaa gaaaagatgg aacttataag cccaggtaaa
    28741 atagtaagat gtgcttggtg tggtagaaga ttttataagt tggataaatc taaagttaag
    28801 tattgcagta gaagttgttc gaagagagca agaaggagtg atttaagtgg ataaagaaag
    28861 tataaaagaa attgtaagag agttaagaaa agaagaaaga gaagatagga aaaaaggcgt
    28921 tttatataat actagattat tgatgaagca ttataatgat tttaagagac atatagactc
    28981 agcggtaagt gaagctaaag atgtagatta cttagaggat gatttaggta agcttgataa
    29041 tgaagagttg tatattttaa gtataaagaa aagtaaagca agaactatta ttatgatagc
    29101 acatatagat tcagcaatga aaactttgaa attaagacaa gagcgtttac attcttatga
    29161 aaagtataga gctttagagc tttactattg tgatgaaaaa acatatgaag aaatagcaga
    29221 gatattaaat tgtggagtag ttacatcaag acgttggatt aatgaaatga taaaggaact
    29281 gggagtgtat ttattcggta tagatggctt gaaattaact gtttgataaa tatatgataa
    29341 aaagatgata ttttaatgat attttaaccc tgttataatg gtagtatgaa acaagtgtat
    29401 aatatattcc cccttgaaaa aggctaagtt accccaactt agtctttttt attttgtaaa
    29461 taaggagaat gataaatgag taattcatga cttagagtga tttctaggtc ttttttatac
    29521 aattttaatc cctcatattg aagggtcgaa aataagggtg ttcaaattga atattcttta
    29581 ttaataacta atttaggagg tagtagtatg ttgaaaattt tacaagagaa aaatgtaagg
    29641 atgatatggt caaaaaatgg agaagaagtt tgatttaatg caaatgacgt aggagaggaa
    29701 ctaggcatag taaatattcg tgatacatta agaaatatag atagagaata taaaaagaaa
    29761 ttaatgagtc tactgtcgga gattcctaca ctagaaactt taaagataaa ttgcctaact
    29821 ttggtactac ttttgttaca gaagaagctg tgtacaatat gtcatttaga agtaataaag
    29881 cagaagcaaa actatttacg aagtgggtta caaaagcact taaacaaatt agaatacatg
    29941 gttactatat tgccacagaa aaagatcaag aatggctgga tataagaaca gaaggtaaaa
    30001 aggtcagaaa agattttaca gatgaaatac aagagtttgt ttattaatac aagcacttga
    30061 aagagttata tctatgaaat cacctaggtt gatagataaa agtatgaatt ataaagaggt
    30121 atataaaaga ttaaagaatt aatagaaatg atttaaatga ctgta
//
LOCUS       DAELTA010000031        29886 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.031, whole genome shotgun sequence.
ACCESSION   DAELTA010000031 DAELTA010000000
VERSION     DAELTA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.031"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            240..1457
                     /locus_tag="KPG13_001219"
     CDS             240..1457
                     /locus_tag="KPG13_001219"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG0984956.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1552..1932
                     /locus_tag="KPG13_001220"
     CDS             1552..1932
                     /locus_tag="KPG13_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0984957.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2216..2698
                     /locus_tag="KPG13_001221"
     CDS             2216..2698
                     /locus_tag="KPG13_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0984958.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2719..3534
                     /locus_tag="KPG13_001222"
     CDS             2719..3534
                     /locus_tag="KPG13_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0984959.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3585..5135
                     /locus_tag="KPG13_001223"
     CDS             3585..5135
                     /locus_tag="KPG13_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0984960.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5232..6584
                     /locus_tag="KPG13_001224"
     CDS             5232..6584
                     /locus_tag="KPG13_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0984961.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6715..7473
                     /locus_tag="KPG13_001225"
     CDS             6715..7473
                     /locus_tag="KPG13_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0984962.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7520..8239
                     /locus_tag="KPG13_001226"
     CDS             7520..8239
                     /locus_tag="KPG13_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0984963.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8363..9763)
                     /locus_tag="KPG13_001227"
     CDS             complement(8363..9763)
                     /locus_tag="KPG13_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG0984964.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9767..10675)
                     /locus_tag="KPG13_001228"
     CDS             complement(9767..10675)
                     /locus_tag="KPG13_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0984965.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10936..11583
                     /locus_tag="KPG13_001229"
     CDS             10936..11583
                     /locus_tag="KPG13_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0984966.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11908..14547
                     /locus_tag="KPG13_001230"
     CDS             11908..14547
                     /locus_tag="KPG13_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0984967.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14605..15708)
                     /locus_tag="KPG13_001231"
     CDS             complement(14605..15708)
                     /locus_tag="KPG13_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG0984968.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16068..16889
                     /locus_tag="KPG13_001232"
     CDS             16068..16889
                     /locus_tag="KPG13_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0984969.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16968..17179
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17266..18582
                     /gene="argH"
                     /locus_tag="KPG13_001233"
     CDS             17266..18582
                     /gene="argH"
                     /locus_tag="KPG13_001233"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG0984970.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18831..19169
                     /locus_tag="KPG13_001234"
     CDS             18831..19169
                     /locus_tag="KPG13_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG0984971.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19296..20585)
                     /locus_tag="KPG13_001235"
     CDS             complement(19296..20585)
                     /locus_tag="KPG13_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0984972.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20665..21369
                     /locus_tag="KPG13_001236"
     CDS             20665..21369
                     /locus_tag="KPG13_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG0984973.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21461..22507
                     /gene="selD"
                     /locus_tag="KPG13_001237"
     CDS             21461..22507
                     /gene="selD"
                     /locus_tag="KPG13_001237"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21509..21511,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG0984974.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22525..23937
                     /locus_tag="KPG13_001238"
     CDS             22525..23937
                     /locus_tag="KPG13_001238"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG0984975.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24051..25934
                     /gene="selB"
                     /locus_tag="KPG13_001239"
     CDS             24051..25934
                     /gene="selB"
                     /locus_tag="KPG13_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG0984976.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26039..28777
                     /locus_tag="KPG13_001240"
     CDS             26039..28777
                     /locus_tag="KPG13_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG0984977.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28896..29840)
                     /gene="manA"
                     /locus_tag="KPG13_001241"
     CDS             complement(28896..29840)
                     /gene="manA"
                     /locus_tag="KPG13_001241"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG0984978.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
BASE COUNT        11630 a         3394 c         5183 g         9679 t
ORIGIN      
        1 taataaagta tatattattg ctttaataag aatgtaaaaa atatttttaa attatcattt
       61 aatgtaattt aaatgaattg aagatatatg tttattgtat gcaattgttg tatttttagg
      121 ttttttgtga ttaattaaaa aattataaaa atcgtttaca tgttgatttt taaaaaaaaa
      181 agatatagaa ttatatagaa agtaataaac aaaaattgga tttacgggag tggaatgaaa
      241 tgatgaatgt taccttaaca gatgtgaaag aggctagaga gaccattaag aacatagtaa
      301 aaagaactga cttacttgaa agtgttaaac ttagcgaaaa aacaggagca aatgtgttct
      361 ataaatgtga gaatcttcaa aaaacaggtt cttttaaatt aagaggagct tgtaacaaaa
      421 tagcgagttt aacagatgaa gaaaaggcaa atggagttat agcttcaagt gcagggaatc
      481 atgctcaagg agttgcattg ggagcaaaaa tgactggtat aaaatctaca atagtaatgc
      541 cagcaactgc gcctttagca aaagttagtg caactaaagg ttatggagca gaagtagttt
      601 taaatggtgc ggtttatgat gatgcatatg caaaagctgt tgaaatacaa aaagaaacag
      661 gagcaacttt cttacatcca ttcaatgata agtatgttat agcaggacaa ggaactatat
      721 ctcttgaaat tttcgaacaa ttagacaata aagttgatac tatactatgc ccagttggtg
      781 gaggaggaat catctctggt gttgctgttg cagcaaaagc attaaatcct aatgtaaaaa
      841 tagtcggtgt tcaaactgca aatatacctt caatgaaaga gtcaataaaa aatggaaaag
      901 taacaacagc atttaatgat actacaatag ctgatggtat agctgttaaa actccaggag
      961 atttaacttt tgaaataata aatgaattgg ttgatgaaat agtagtggtt gaagagacag
     1021 aaattgcaga atcaatatta tttatgatgg aaagtcaaaa aatagtttca gaaggtgctg
     1081 gtgctgtatg tacagctgct atattaagtg gtaagtatgt accagcaaaa gatgaaaatg
     1141 tagtttgtat aatatctggt ggtaatatag acataaatac gttatataga ataataggtg
     1201 ttgctttagc taaagaagga agaagatatt catttagtac aattatggaa gataagccag
     1261 gaaactttgc tgagttaact agaataataa gtgagaatgg tggaaacatt ttaagtgcta
     1321 atcaaggtaa attatcagca ggggaagctc taggaaaaca aagtgcggaa tttatattag
     1381 agacaataga ttatgaccat atagctagaa taaagaaagc tatagaagaa aaaggtttta
     1441 aaattataga attataataa aaagtgtata taatatacaa gaaaaatagt ataatagtgt
     1501 atgaagatac attttatgtg tattagatta acgaattagg aggattttaa aatgaaacat
     1561 gaagtaatac atactaatga tgcaccagca gctttaggac catattctca agctataaaa
     1621 gctggaaact tgttatttgt atctggacaa gttcctcttg taccagagac tatggaagta
     1681 gttgaaggag atgtacaagc tcaaactgct caatctttaa aaaatcttaa agctatatta
     1741 gctgaaagtg gagctgattt ttcaaatgta gttaagacta cagtatttat aaaagatatg
     1801 aatgaatttg gggctataaa cgaagtttat gctgaatatt ttggagaaaa taaaccagca
     1861 agagcttgtg tagaagttgc tagattacca aaagacgtta aagttgaaat agaagtaata
     1921 gctgtattat aaaaatacaa tatttttaat ttgaaaaggc tgaaaaacta tattctagtg
     1981 attcagcctt tttataaaaa aataaatcac aataaagaac atgataaata ttgtcgaatt
     2041 aaaaaataaa attaaaataa atattgcaat atttttgaga aggttttata atgagtatat
     2101 aatttgaaac gtgtaactat taaattaggc atgagttctt ggatattttt atcctgagga
     2161 ttcatgcctt ttttgtattg aaattcaaaa atctatttaa taaggaggaa gtactgtgtt
     2221 aaattttttc aagaaaaata aaagctataa gttacatgca gtagtgagtg gtaacagcat
     2281 taatattgag aaagtaaacg attctgtgtt ttcaaaaaag ttaatgggag atggagttgc
     2341 aattattcct aatagtaatg ttgttgtagc cccttgtaat ggtaaagtaa ctgtgcttac
     2401 agaatcaaag catgcctttg gtatggtctc agatgagggt gttgaaatat tagtccacat
     2461 aggaattgat acagttagtt tacagggtga ggggtttaaa aatgaagtgt ctcaaggaga
     2521 tactgttaaa aaaggtagtc caataataag ttttgaaaga gaaaaaataa attctcaggg
     2581 aattgattgt acaacaataa taattgtatt aaatcatagc gaattttcag aaataaattg
     2641 catggtagaa aatgaagttg ttgcagggca agatactgtt attgaaatta tgaaataata
     2701 ggaagggatt aattcaatat gagagtagta aaaatcttta ataataatgc attatctaca
     2761 gtaactaatg ataggaaaga agctatttta ctaggtcttg gtataggttt taataaacgt
     2821 ccaggagata aagttaatga agataaaata gaaaagattt attatgttca agaccacatg
     2881 caaacaaaat ttttagaatt attaaaaaat gttagtccag aagtgatgga tgcttcacaa
     2941 caaattataa gtcttatacc aggaaatgag ggaaaattta ataataaagg gatattatct
     3001 ttagtggaac atattagttt tgcaatagat agaatgaaaa ataatgtttt tttacctaat
     3061 ttaatgcttt tggatataaa gatgatgtat tcaaaagagt ttgaattagg agtaaaagct
     3121 ttagaaatta tatatagagt atgtcatatc catttgcctg aagatgaagc aggttacata
     3181 gctttacatt ttgtcaattt acaatcaaat gataatttgg catatgatac tttaaaattt
     3241 gtaaaaggta gtattgattt gattaaagaa tgttatggat tagaattaga cgaatcgagt
     3301 ttaagtacat tacgttttag aactcatctt aaatttcttg ctcaaagaat ttttaagaat
     3361 gaaatttgtc aggatgataa aatgattgaa atgtatgatt atttaattaa caatcatcct
     3421 aaaaataaag aatatttaga aaagttaaat gtatatattg aaaaggagtt taggtataaa
     3481 ttagataaac ctgaaaaaat ttatctacta ctacacctaa caaaaatctt ataagaatga
     3541 gatgaattag taatagaaat atataatttt agggggggat aagattgaaa gaaaaactac
     3601 ttggaggact ggaaacattt gcaaaggcaa tggttcagcc attaatgtat ttatcgagtg
     3661 caggaatatt aatggtggta ggtgtactac ttacaaatga tatgttatta ggagtacttc
     3721 cattttttaa atgggagcca attcaaatat ttggaagcct aatttataac tgtataatgg
     3781 ctattattaa taatcttgct gttatatttg ctataggtat tcctgctgca ctagcaaaaa
     3841 aagagaagca caaagcagcg ttaataggat ttatgagtta tataatatac cttacagctt
     3901 ccaacactat gttaacatca ctaggtcaac tagcaaaaga aagcaagatg cttggactga
     3961 tagggactgg acaagctact attttaggta ttcaagttgt agatacaagt gtatttggtg
     4021 gaattatttt agggtgtata gctggatatg tatttaatcg tacaagcaac aaaacattta
     4081 aaggagcttt acagatatac tcgggagcaa attttagttt tatatgcatg ttctttgtat
     4141 ccattttaat tggagttgtg agtaacttta tatggccatt agctcaacaa ggaatagctt
     4201 ctcttgcaaa tgtcatcaaa gatacaggta catttggttt attcttatat ggttttttag
     4261 agagaatatt agtaccaact ggacttcatc atttaatcta tacaccattt cagttttctg
     4321 atttaggagg agtgcttaaa gttggtgcag atacagtttc aggagcctat gctatagtta
     4381 tgacagaaat gaatttacca gttgagcgtt tctcagatag tatatactac atggcaactg
     4441 gatttacaaa gatgtttggt tatataggta taggagctgc ctttatttat actgcatata
     4501 agcaaaataa gaagaaagtt aaggcaacga ttattccact aataattacg gcagtattag
     4561 cctctgtaac agaaccatta gactttatgt ttgtattctg tgcaccattt ttattctttg
     4621 tacatgcagt tatagctggt ctttttatag ctttattaaa aatatttgat gttactgcat
     4681 tatgtggagg gaacttatta gtatcaatta taatgaatac tacagtaggc gtagaaaaaa
     4741 cacactggcc agtaatgatg atattaggac ttgcacaaat tattgtatat tttgtagtat
     4801 ttagtttttt aattaagaaa tttaattata aaactccagg tcgtgaagat gagagtacag
     4861 ctacaggcga agaacaatca aaacaactta atttagatgc agggatagaa aatataattg
     4921 atgggttagg cggaaaagaa aatattaata cagtagaaaa ttgcatcact agattacgtg
     4981 ttaatgtaaa agatgaaagt aagattaatg aagatataat taatttaacg ccaaatagcg
     5041 ggattgtaag gaaaggaaaa gatatacaaa taatttttgg acttcatgtg catgaggtaa
     5101 gacgtgcagt agaagatttt ttagaagctt attaaagata tatatttata tagcaagggt
     5161 aaataacagc tttaagtgct gtttactatt tattaaagat acatataatt tatagaggag
     5221 gaataattat tatgattatt actattgttg gtggaggaag cacatttact ccaggtattg
     5281 ttaagtcaat tgcattaaga gaaaaagagt tagagcttga ggaaattcgc ttatttgaca
     5341 ttgataaaga acgccaagat aaagtagctg tagttgtcaa ctggatacta aaagaagatg
     5401 ttaaaacaga tgttaaacta actgtaacta attgtgaaaa agaagcgtat acagatgcta
     5461 catttatatt tgcacaaatg cgtgttggta aatatgaaat gcgtgagcag gatgaaaaaa
     5521 ttccattgaa atatggttgt gtaggtcaag aaacttgtgg ttgtggaggg atggcatacg
     5581 gactgcgtac aatacaacca atgataaagg ttattgatga tgtagagaaa tatgctaaaa
     5641 aagattattg gatattaaat tactctaatc ctgctgcaat cgtttctgaa gcttgtcgta
     5701 agttaagacc aaatgcaagg attataaata tctgtgatat gccaatagct attatagata
     5761 tgatagcggg gtcattaaat atcaatgacg ttcataatat acgctatgat tattttggtc
     5821 taaatcattt tggctggttt acttcaatag attataaaca tagagactta atggaagaga
     5881 ttaaggaata cataaaagaa aataaaatat tattaccaaa atcttatata gaaaacttaa
     5941 ataaaagtcg tagtacagga aatcgccatg ctgtaagtag ctggatacat gtttgggaat
     6001 ctgtttatac aatgttagaa agcttcccaa aatatctgcc aaacacttat ttaacatact
     6061 atcttttaag taaagaaaca gtggaacatt caaacataaa tcgtactcgt gcaaatgagg
     6121 ttatggaaag ccgtgaaaaa gacttatttg aaggtgtgag acattactta gaaactggag
     6181 aaatttctga aaaagcattt tatgcaggtt cacatggaga ttggattagt gatttggcag
     6241 tttcaattaa aaatgataca cgtagtcgtt ttttagttat aactgaaaat cgtggagcta
     6301 taccaaacat gccatatgat gcaatggtag aaattccagc atatattggt aaagatggtc
     6361 cagaagttat tgctcgaggt ccaatacctc tatttcaaca aggattaatg atgcaacaac
     6421 ttaacagtga aaaattgata gtagaagctt atatagaaaa tagttatgaa aaagctttac
     6481 aagcattcac attaaataaa actattccaa gtatgaatgt tgctaaagaa attttggatg
     6541 aaatgataga agttaataag gaatactggc cagaattaag atagtaggat aaaggaaagg
     6601 gtttttattt aataattaga tatgttttca gagtctttta tcaaaatgta ttcacaaaca
     6661 tatggcaata tattaaaata aagagtgata tttttagaag aatggggtga tgcaatgaac
     6721 attataaata aaattttttg ggaagaagat aggattggca tgattacaaa taatttagag
     6781 gcagatacac atagccactg tatgttacaa ctgttcttag ggatagaaga cagcattgaa
     6841 ataacagtca atgaaaaatt agttaaatgt aattgtatca tagtagataa aaatatctct
     6901 cattcatttt cagcaagaaa aaaagtttat tactctgcta ttattgaacc tacatctata
     6961 tatgcagaac agcttacctc aaaaatgaat gattttggct actggatatg tgacaatgat
     7021 ggattagaga aattacgtca acaaggaact tttttgatag ataactctag taaagaacaa
     7081 tatttaagat ttatggaaat gcttaataac tatttgaata tacctataac tctcaaacat
     7141 tatgatgaca gaattactga attgctcaat ttattacgta cttgtaattg tgataatcat
     7201 actatttcaa gttttgcaga taaagtgtcc ctatcagcaa gtagattatc acatctgttt
     7261 aaagaacaaa tagggatacc tttaaaaagc tatatcttat ttcatcaaat ggagtgtgct
     7321 tttagagaat tactttctgg gaaaaatgtt accgaagctt ctatgactgc tggattcgat
     7381 actccatcac actttgcagg gacagttaag cgaatgatgg gaatgcctgt ttctttatct
     7441 ttgaaagata gcgaattttt gaaagtatat taaatattct cgttatataa tgaagttgta
     7501 ctaagaaagg ggctttgtta tgaataaaga atatttagag gaaacaaaaa tgttaaatta
     7561 taatgaacca caacttaagc ttttggtatc atcaaagaat tggttggaat tagatgattt
     7621 tcataaaata aaatctattt atgagtttgt acagaatgat attttatttg gatataatgc
     7681 ttctgatatg ttaagcgcaa cacaggtatt aaatgatggt atgggacaat gcaatacaaa
     7741 agctactctt ttaatggcat tattacgtgc agtgaatatc ccttgtaggc ttcatgcttt
     7801 tgatgtaaca aaagattttc aacgaggagc tacatccaaa ttgatttcac tattagcacc
     7861 aaaatatatt cttcatactt gggtagaggt attttatcaa gataggtgga ttgctcttga
     7921 gggtgtcata actgataaaa aatatttaga agcaattcag aagaaatttt ttaatcatgg
     7981 tggaacattt aaaaagtatg caatagctac aaatgatttg aaaaatacat ctattgattg
     8041 ggatggtaaa gatactttta tccaaaaaga agctatcgtg tatgattatg gtatttttcc
     8101 atcaccagac gtattttttt ctactcattc acaacatatg tcaaaattaa aaaatttcat
     8161 atatgtacat cttatcagaa aaataatgac aaaaaatgta tgtaaggcta gaaacaatta
     8221 tattgataaa aacgaatgag tgtttaagag gtaactatat aggaaatata tagaataatt
     8281 aaaactgtac tggttaaaat gaataaaaag gagttatcac agagcaaact taaattgttc
     8341 ataggataac tccttttctg tattaacttc taataatttg atgtcttttt ataaaacata
     8401 tagatacaat taaaccagta aatgcaagag tagctgctat tgaaaatgca acatctattc
     8461 catgaatgtt agacagcatg tttgaactca tatgagcttt cttagtagca cttgtcatta
     8521 tagtaataag tatagcagaa cctatagctc cagatatctg acggagtgtg ttattaattg
     8581 ctgtggcatg agatataagt tctctatcta atgttttaat tccccatgta gttaatggca
     8641 ttaaaaccat tgaaatacca atcatacgaa tacaatacat caatgacaca tgaattaaag
     8701 atgtatctct gcctaaaaaa gcgaaggata aagttcctaa aaacaaacat cctgttccta
     8761 atattgatag agcatgaggt ccatatttat ctaaatgacg tcctgcaatt ggattgagta
     8821 caaccatcaa tatagcacca ggtaacatta atgaacctga agataatgca gaaaatcctc
     8881 ttacagattg aacgtacaga ggaatcatta atgtagcaga catcattgag gcatatacaa
     8941 tcataattaa tattgtagaa ataagaaatg gtttattttt aaaaaccctc aattctaaaa
     9001 aaggttcttt agattttaat tgacgcaatg taaataaaat taatgacata attcctatta
     9061 atattggtaa atatgtggca atattagtcc acccataatt accttgatta gtaaatccaa
     9121 ttaataaacc accaaaacct agagatgata gtactattga aggtatatct aattttagtt
     9181 tttgagtttc cccaacattt cttaatagaa taaatgatag tataacatct aatatagcaa
     9241 taggggaaag aaacagaaat aaactatgcc aaccaaatga atctactaac catcctgata
     9301 aagttggacc tattgctgga gcaaatccta ctgtaatacc aactaaactc atagctgcac
     9361 cacgtttttc ctcagggcat aaatagagta taataacttg taaaagtggc attagagaac
     9421 cagaacccat tgcctgcaac acacgagcaa tcaacatagt tgaaaaatta ttagaaaaaa
     9481 gtgcaataat acatcctata gaaaagatag acattgatgt aataaataac tttctagtac
     9541 taaatcgatt tattaaataa gctgttgtgg gtacaataat acctgcaata agtagataaa
     9601 ttgttgttaa ccactgacca atatcagcag taattttaaa atctgacatt atattaggta
     9661 atgctgttga caatagtgtt tgactgagag aacttataaa agcaccagtc attacaattg
     9721 ctattattaa atttcctttt ttattgttac tttgggtttc cataatttat aacgtctcct
     9781 tttttaattg atttttaatg ctgttcaata aaagtacttt tggtcttaaa gtgtcttctt
     9841 tttctaaata tgcaatcatt gcatccataa ttaaagaatc atattgttga ttttttcctt
     9901 cgacgtataa tttttttatt gcttctagta atttaggatt ttcaaggaga gaagtatcag
     9961 ttttgattag ttcttctata tcttgtatcg ttttaaaaaa tagcgattta ttatgaaggg
    10021 aaaagtaaac ttcatcttct tgtttatgta aaaacattga tttttcaatt aaaggattta
    10081 gattatccat attttctaca actaaatcta gaatttgtat agtaaatttg gcaataaagt
    10141 atacatttat ttctcttttt tgtacatgaa taatgtatat aaactgatga attagagcat
    10201 ccaataagaa aactaaatca aatataattt tttctatttt atcaccatag ttatcaaata
    10261 agcttagttt gtattcattc aataagttag ctcttgattt tgtttgcaat ttacttgtag
    10321 ccttacgttg ctcttcagaa aagttttctc ttatataggt atttatggta gttctagaaa
    10381 aaatattttt ccatacagca attatctttc tgtttaactt ttctgatgat ggaatgtcat
    10441 tattttcatt tatggaatgt ataatttcta aaaacttttc atcataataa ttgattattt
    10501 cgcagataaa actttcctta gtttcaaaat gcttgtaaaa agtagcttta gacattttac
    10561 actcttttac aacatccgat attgaagtat tttgaaaacc tttttggtca aaaatttttt
    10621 gtgcaatagt tataatgagc ctttgttttt ctgtcacttt attatctcct ttcacgcaaa
    10681 aacgtttagt actatatagt acctacaagt tttgataaat atattataca tcatttggaa
    10741 aaaaaatcaa tatatagact gtaatatgaa atttttgaaa aatttgttgt tatttttagt
    10801 cgtataaaaa agaaacttaa gagtttctta agaattgttt aaagaagttg caataaatta
    10861 gtggtatgat tatagtatga aagagaatgt cttgaaattt ttctgtatca aattccaatt
    10921 atggaggtgt agtatatgaa aaagaaaatt tgtgtagcaa taatttttgc tctatttata
    10981 gtttttgtag aaatgtttat tgatagctct agcgaaggaa gtaaaattat gacagctgaa
    11041 ttaagtaaat acaggaaagg cgtaaatgta ttatcaataa acaatattat agtagcgaat
    11101 aaaaaatata gtttgcccaa agattattct cctcaggaaa gcagtgaagc gagagatgct
    11161 ttttataaaa tgaataaaga tgctcaaaag tcaggattaa atttaaaagc ttttagtaca
    11221 tatagaagtt atgaatatca agatagatta tttaagtcat atgtaaaaga acatggagaa
    11281 aaagaagcaa atagattttc agctaaacct ggagagagtg agcatcaaac tggacttgct
    11341 tttgatattg gaggagatga ccagtcttgt tgggcaaata aaaagttcaa caatacaaaa
    11401 gaagctaagt ggttgtatga aaatgcatat aaatatggtt ttatacttag atatccagaa
    11461 ggtaaagaga atataacagg atatatgtat gaatcttggc actatagata tgttggaact
    11521 gaacatagca aaaactttgc aatgaataat ttgactttag aagaatattt acatataaat
    11581 taagagatgg tttgttaaaa ttaggttaaa ataaaaaata acctagacta ttagctgtgt
    11641 aaagtgatat aatgtaaata caatttaaga taactactcg aaggggagta gcttacacga
    11701 aagtggtaat taatcgtcaa gacagtagaa atactcggtt aattagcaag ggattccttg
    11761 atagcaagac cttcaaaata agcattaggt atgtttgttt tggggtcttt ttttattcaa
    11821 ggaattttta tttaaaaaaa tttaaatttc aagtatacat aagagccccc aaacataaaa
    11881 taaatttatt gaagaggagg gcaacaaatg tcttacaaaa gtacttatca agaagtaatt
    11941 aaaaatttag actcaagttt atctggtttg agtaaagaaa gagcagaaga gttgttagaa
    12001 aaaaatggag ctaatgaatt aaaagaagca gataaggttc ctacttataa gcttttccta
    12061 gaaagtttta aagacccatt agtaataatt ctattgatag ctgctttagt acaaatattt
    12121 ttaggtgaaa cagtggagtc tataataatt tttgcagtta ttattataaa ctctgttctg
    12181 ggtgttgtac aaacaaaaaa agcagaaagt tcattagaaa gtttgaaaaa tttatctgca
    12241 ccaaacgcaa aggtaataag agacaataaa aaaatgacaa ttccagcaaa aaatctagta
    12301 gttggagata ttgtattctt agaagctggc gattacatac cagcagatgg aaggcttata
    12361 gaagcacaat ctctcaaggt tgtagaaggg atgttaactg gagaatcaga accagtttta
    12421 aagcatatag aaaaaataag tgaagatgta agtttaggtg accagaaaaa tatggtattt
    12481 agtggtgctg tagttgttta tggtagaggt tcttttgtag tgactggaac aggtatgaat
    12541 actgagatgg gtaaaatagc tgggctttta gaaagtgctg aaaataaact tacaccattg
    12601 caacaaaaaa tagaggattt tagtaaaaaa cttggtgtag gtattgtaat acttgctctt
    12661 ttaatattta taattcaagt agcaagaaat tactttatgg ctggaaatgg agaaatgaca
    12721 caaattattt tagattcatt catgttctct gtagcagtag cagtagcagc tataccagaa
    12781 gctctatcat caatagtcac tatagtattg gctgttggaa caaatagtat ggcaaaaaaa
    12841 caagccataa taagaaaatt accagcagta gaaactttag gttcaacaag tgtaatatgt
    12901 actgataaaa caggtactct tacacaaaat aaaatgacag tagttgatta ttatatgtat
    12961 ggaactaata atgatgagtt agataaagaa aaagtaaact attcttatca tgctaaatta
    13021 ttgactatgg tttctacatt gtgtaatgac tctcatataa ctagtgatgg taaagaagtt
    13081 ggtgacccaa cagaagtggc tttaataaat tattcaagta aaaatgattt agattatgat
    13141 aaattaagaa gtaagtatat cagaataaat gaaataccat ttgactcaga cagaaagctt
    13201 atgtctactg taaatagtat atatggagat tatataatgt ttacaaaagg tgctccagat
    13261 attgtgttta gtagatgtaa atatgctttg aaaaatggtt caaaagtaga tataacagat
    13321 gagataatag aagaatataa acataaaaat gaagagttct caaatagagc acttagagta
    13381 ttagcttttg caataaaaga tgtcccagaa gaagatttta taccttcaat tgatgacgaa
    13441 catgacatga cattggttgg aataatggca atgatagacc caccaagaga agaagtaatg
    13501 gatgcagtaa aagaggcaaa gagtgctggt ataaaaactg ttatgataac tggtgaccat
    13561 aaaacaacag cagcagcaat tgcaaaagag attggtataa tggaagaagg agacttggct
    13621 cttactggaa aagagcttga tgctttgagt gaagaacaac tatatgaaaa actagaaaat
    13681 ataagtgtat atgccagagt ctcgccggaa aataaaattc gtatagtaaa agcatggcaa
    13741 cataagaata atataacagc aatgactggt gatggtgtta atgatgcacc tgcattaaaa
    13801 caagcagata taggaatcgg tatgggaagt ggtactgatg tagcaaaaga tgcttctgct
    13861 atggtattgg ttgatgataa ctttgcaagt atagtaaatg ctgttgaagt tggtagaaca
    13921 gtatacagta atataataaa atcaattact tatctatttg caggaaactt aggagctata
    13981 gtatccatag tatttgcagt gtttgcaggt tggtcaaatc catttacagc tttacaactt
    14041 ttgtttataa acttagtaaa tgactcttta ccagcaattg cattagggtt tgaaaagtca
    14101 gaaaaaaata taatgaataa acctcctaga cacccggatg aaagtatact agcaggagga
    14161 acattgaagg cagttgctat aagaggttgt ataataggtg tagttacaat aattgcacaa
    14221 tatataggat tacaagtatc tccagaactt ggagtagcaa tggcattttc aacattgata
    14281 ttagctagaa taatgcaaac attacctgca cgttctaata atcaaacagt aataaaatta
    14341 ggttttttca gcaataaata cgttatagga gcagttttag tctgtcttgg gctttactct
    14401 ttaacactaa taccagtgct aagaggaata ttctctatac caacaacgtt tggattgtac
    14461 cagttgggga tttgcctagg gttagcatta atttcatcaa tattaatgga acttaacaaa
    14521 ttaataaata gaagtgaagt aaaataatca aaaaattaaa gggcatattg attttaacaa
    14581 tatgcctttt ataaatagtc taatttattc tatattatcg attatttgga atgcttttat
    14641 aacttcaggt aattcttttt cttcaggcat tctaaatctt ataaaatttt tatcaagtcc
    14701 agtaaaatca cttccagata taactcttat ttttaattta gcaaactctt gttgtaaatc
    14761 tatgtccttg tttttatgtt caactgtcat aattgataca gtatcactag tttcagctat
    14821 attaaggttt ttccatggaa ttaataattg attttttata tcttttgttt tttcttttaa
    14881 ttcttctata aattgaacat catctaataa atttgctgca atactacgag atatttcaga
    14941 cacttcgtaa ggagtagaaa tctttttaat agggctaaca agttgttctg gaagaactgc
    15001 atatcctgct cttagtcctg ctaaaccaaa tccttttgaa aatgttttta aagcaattac
    15061 attatcataa ttatttagaa gcttgacagc tgaattttct tttggcatgt attcaccata
    15121 agcttcatct accataactg ctatatcata tttagctgct tcccttacta tattttcaat
    15181 tgatgataaa ggtataattt gtcctgtagg attgtttgga ttgtctatgt aaattacttt
    15241 atattctgga tttatttttt caataaattc tttttcatta aacttgaaat tatcctcttt
    15301 ctttaaaaga acataatcat aagttgcacc atgcattttt atatctgtct catattctga
    15361 gaactgaggt gaatatccta aaactttatc tcccttttca ataaatagtc tatttaaaag
    15421 gtaaataaca tgtatagaac catctgccaa acaaattctg tcagtatcta atgctataaa
    15481 atctttccaa taatcgacta tattatcttt taaagcaatt gaatgaggat atcctcttat
    15541 catatcaaat tttaaagcat taaaagcttc tacagctttg tttgagtaag acacggtatt
    15601 tataccttca ccacagtcta tctcgaatac atcttctgaa tgtccagatt ctattgcata
    15661 actttttttc aactgcattt caatagcttt gttaattttt agtttcatac tttatcactc
    15721 cttatttagt caataataaa tgttataaaa gttgctagat ttaaaatatt atgaaaatat
    15781 ataatagtaa aaatgaatat aaatattata atagctcact ttatttaata tgattatata
    15841 tcatatattg taaaatttct agcattataa gactataaat tgaaaatatt cagaaaaata
    15901 ctacaagaaa cgatttattt tgctcttaaa ataagttaaa tgaatgttat taattttgta
    15961 aattgaaata gtttaagtat aaagttatga tataatctaa taagggtaat tctttagatt
    16021 atattaagga aatataggta tacaataaaa agttatggag ttggattatg ggatataaat
    16081 taattgtaac agatatggat ggaactttat taggcaataa tcataaagtt acagatgaaa
    16141 ataaaactgc actacaaaaa gttataaaaa gcggtattaa tgtaacttta gcaacaggaa
    16201 gagccttcga ttcagctaaa tgcaatgtag attttcttaa agaggacatg ccaataatag
    16261 cttgtaatgg ttcattaata agagagcaaa atggaaatat aatatattca aataagattg
    16321 atacaagaac atgtttaaac attttagatg tattagataa atacgatatt tactaccaat
    16381 gtaatagtat agattctatg ctttcgaaaa aaatagaagg acgtgaagat agattaagtg
    16441 tatttttagg ctctgaaaca gaagtaattg taaaagatga tttgagagaa gaaatcttta
    16501 aaaaagatat actaaaattt gtaatcatag aagaaaaaaa tccatctatt ttggatgaaa
    16561 taagaaaaga attgagaaaa gttcaaggaa taaagataac ctcttcatgg ccaaacaata
    16621 ttgaagtcat gaatgaaggt gttgataaag gtaatgcagt taaaatttta gctgaaaaaa
    16681 tgaatataga cagagaagat ataattgcat ttggagacaa ttacaatgac atagaaatga
    16741 taaagtttgc tggtcttggg gtagctatgg gaaatgcaga agaattgatt aaacaagaag
    16801 ctgattatgt tacagataca aatcaagaca gtggagttgc aaaggcaatt tataaattcc
    16861 tagagttaaa agaatcactt agctcttaaa acagtatata ataggaaaaa ataggttgac
    16921 tattttgtag atagtaatta taatacaaat agataaaata taaaaattta gattcagtga
    16981 aaaagagagt agatatagag aaaatctcag cgaattgggg gatggtgtga gcctaatgat
    17041 aacactttat attgaaaaga actttggaga atactacttg aaattcagta tggtagtagg
    17101 taatctcgcc ttaagagaat agagtgggta aacattgttt accaactaga gtggtaacac
    17161 ggaatatttc gtctcttggt ctttttgacc aagagatttt ttagtttcat tgagttacaa
    17221 gacttataat taaaatacaa aaaattaaaa tatgaggtga aagttatgaa attatggggt
    17281 ggacgcttta gaaaagcgga aaatcaactt atggaagaat ttaataaatc ttttggctat
    17341 gattgtgtac tgtacaaaaa agatatcgaa ggaagtgtag cacatgttca tatgcaagta
    17401 aaatgtggac ttttaacaga agaagaagga aagtctataa cagaaggttt aaaagggata
    17461 cttgaagatg tagaaaatgg aaagttagta ttggatggag agtatgaaga tatacatagt
    17521 tttactgaga taaatttaat tcaaagaatt ggagatgttg gcaagaagct tcatacagca
    17581 cgtagtagaa atgaccaagt tgcagttgat atgagattgt atgctaaaga aaaagcaaat
    17641 gatttggtgg atttaatatc tgaatttaaa gctactataa aagatgttgc tgacaaaaac
    17701 ccagtgatga tgccaggcta tactcatctt caaagggcac aagtagtgac atttaagcat
    17761 cacttaatgg catattatag tatgtttgat agagatgaga aaagaataaa aaatgctata
    17821 gaaatattag atgaatcacc tttaggctgt ggagctttag caggaactac gcatgatata
    17881 gatagaagta taacttgtga acaattagga tttaaaaaag tagtagataa ttttatggat
    17941 ggagtaagtg atagagatta tttactagag ctaatgagtg atttttcaat tataatgatg
    18001 cacttaagta gattaagtga agagttgata ctttggagtt ctcaagaatt tggatttgtt
    18061 gagatagatg atttatatac aactggaagt agtataatgc ctcagaagaa aaatccagat
    18121 ggtgcagagc ttataagagg taagactggt agagtatatg gaaacttatt tggtcttttt
    18181 acagtaatga aaggaatacc attagcatat aataaagaca tgcaagaaga taaagaaggt
    18241 tttttcgata gtgtacatac tcttgaaatg tgtatacaaa taatggatag aatgatagcc
    18301 acactaaagg taaatgagga taaaatgaaa caagcagtaa aaaatggttt tttaaatgca
    18361 acagaagttg cggattattt ggttaaaaat aatgttgctt ttagagatgc acatggtata
    18421 gtaggaagta tcgttatata ttgtgaagat aataaaaaag caatagaaga tttaacatta
    18481 gaagaattac aaaagttttc agatgctttt aaggaagata tttatgattt tatcgactat
    18541 gagagtatat taaataaggg aataaaaaag aatttaaaat aatgtattta tttttaaaag
    18601 ctgtaaattt gaatgttatc atttaaattt acagcttttt attgtgtaag attttattat
    18661 ataaaagtaa gaaacttaaa aggagtagta tttaaaataa atacaaataa aaaattatta
    18721 ttaaaatgat aattaatatc aataagtatt gacaaaatta tttctaaata ctattatata
    18781 taatataaat aataatgaaa atcaatatca aataaatggg aggaaatcta atgaacttat
    18841 tagtgattgg tggtcatgaa agaatggaaa aagattatat ggttatggcc aagaaaaaag
    18901 gttataagac aaaggtatat actaccatgt catctaaatt aaataattct ataggaaaac
    18961 cagatgcagt tgtcatactt acatcaactg tttcacataa gatgtctaga acggttgagt
    19021 ctcaagctaa aaaacaagat atattaatcg taaggcataa aaatagtagt aaggtagcat
    19081 ttaatgaatg tctagatatg gttgatgaat gtctgggaaa ttgttcaaaa tgtaagctta
    19141 aagacaaaca aaaacaaaaa aatatatagg tataaaaaat acataaacat aaaattaaaa
    19201 gtactgagat aatagttata attattatct cagtactttt agatttataa ggataagtat
    19261 agtataaatt ttttattata atttccaact acttgttact ttttcatatt ttttttactt
    19321 ctagcttttt ttcttttttt cttctttata tttgaagcta ttataaataa aactaaaagc
    19381 actagaatac ctgcaataat cttaacaaaa gtcattaaaa cattattttc agtcaaaaat
    19441 ggaagactgc tatcaagatt attttttgca attaagtcta tactgttttc tagtttacca
    19501 ttattatata catttaaagt accaacctta gctccttttt ttataggcaa atcaatttta
    19561 tttaactctg ttttggtatc ataattgcct tgagattcat ctttttctaa gaatatccta
    19621 taactatttt ttggttcata aatcaattca tgttgttttg taaacaatac ttttttagta
    19681 tctgtgtatt tttctttatc aacaatagta gcacacttaa agttatcaaa tccataatca
    19741 atcagaatac gtgagtctaa atataaatca gcattagttg aattaaatac agcaacgata
    19801 actcttctgc catcttttac agcactcgat aaaagacatt taccagcagc atcagtatat
    19861 ccagttttta taccatcaac tatatcatat tttatattta taggttgacc tttgtatgta
    19921 atcttatcat gtgatgtcaa aaacttatta gtgtttacaa agtatctctc ataaggatat
    19981 gcttttgtag cttcaaattt aagtgactta gtttttacaa tttgtctaaa agtatcatta
    20041 ttcatagctt ctctagctat gagagccata tcatgagctg ttgtgtagtg atttgggtct
    20101 ggtaatccat gaggattatt aaaatgagta ttagtagctc caattttttt tgcctcagag
    20161 ttcatgaggt caataaattt ttgtacattg ccaccaccaa catatctagc taatacaaaa
    20221 gcagcatcat tagcagaatg aaccaaaaga gcatctagta actgctttac agtaaaagat
    20281 tccccatttt ttagatacat actagagcca tctatttgag gtaaatcttt aatttctatt
    20341 acttgattta aatctttaac ctcctttaga actaaacaag ctgtccatac tttagttgtt
    20401 gaagctggat aaagcttaga atttccatcc ttattatata gtattttgcc agactcatag
    20461 tccattaaaa ctgcatgttt agcaactata gctggttcat tatctgcaaa agagatatta
    20521 gagcttccta aaaatgaagt aaatatcaat aaacagatta ataaaagtga taaatttctt
    20581 ttcattgtga gcctccaaat tttaaattca tcttttagta tagcatatat ttgtcaataa
    20641 ttaaaagaaa ggagtgatta atatatgaaa aaaaaggtaa aatttatttc gttttttgtt
    20701 ttatttattt taataatatc atcaataatc gtttataaat cgcttatttt caaagatgat
    20761 atttatgttg taagtaaaaa aaatttacct aatgaaaaaa cacatgttgt agagaatgaa
    20821 attaaaggtg aagataataa tcacagtaaa gtaactaaag acatagataa aaattttcaa
    20881 aataataaaa taactatata cataagtgga gcagtaaata gacctggaat tgtaactata
    20941 gagtcagaca agagacttta tgatgctgta gaattactag gaggaacgac aaaagaagca
    21001 gatttaaatg gagtcaatct atcagtaaga ttagaagatg aacaacatta cataatacct
    21061 aaaataggtg aagcaacatc tgttacaagt aatgatgatt ccaataaact aaatcagaag
    21121 aatgagtcaa gaggtgagcc taaaaataaa tccaataaaa atatatctaa cgaatcaaaa
    21181 gtaaatataa atgtagcgac gatagaagag ttggattctc tacctggggt tggagaagct
    21241 acagccaata aaattttaca acacagagaa gaaaatggac agtttagttc aattgaggaa
    21301 ataaaaaatg taaatggtat tggagataaa aagtatgaaa atataaaaga tttaatttgt
    21361 gtagactaaa atataataca aaacttacat atagatatgt tattataaat atgtatgtat
    21421 atgtgtgtac atgtggaaat ttagtaaagg aggcactttt atgaaaaaag aacaaaaaaa
    21481 acttacagag atgacaacag caggtggttg agccgctaaa atagggccag aggttctggc
    21541 atcagtttta tctcaattgc ctaaaaatga taatatagaa aatttattag taggactaga
    21601 tacagcagat gatgctgcag tttataaatt aaatgatgat atggcactta tccaaacttt
    21661 agacttcttt acaccaatgg tagatgaccc atacgtattt ggtcaaattg ctgcatcaaa
    21721 ttcactatca gatgtatatg ctatgggagg caaacctttg gttgctatga atattgtttg
    21781 ttttccatca tgccatgata tggatgtact tgcagaaata ttaaaaggtg ggtttgataa
    21841 agtgaaagaa agtggagcac ttttggttgg agggcatact gtagatgaca aagagcctaa
    21901 atatggtctt tcagtttctg ggattgtaag tccaaataaa gttttatcaa atgctactgc
    21961 aaaacctggt gataagttaa ttataacaaa accaattgga gtgggtgtat taaatacagc
    22021 catgaaagaa ggtatggtag aacaacatat agcagataaa gtaatagaaa taatgataca
    22081 tttaaataaa tatgcagcaa tgagttttga gaaatttgat gtaaattcgg ttacagatat
    22141 aactggtttt ggtctacttg gtcatacttt agaaatggcc aaagcttctg aagtaagtat
    22201 tgaaattgaa tcaaaacatg taccaattat tgaaggtgca atagaaatgg cacaaatggg
    22261 aattatacct gctggtatgt ataaaaatat gcattatgta tctaaagatg tagaagttgt
    22321 aggaaatata gaagttgcag ttcaagatat tttatacgac ccacaaacat caggagggtt
    22381 gttaatttca gtaaaagaag aacttgcaga ggagcttgta aaagatatga aattaaatgg
    22441 agcaatagaa gctaaaatta taggttctgt agttccaaaa ggggaaaaat atattacagt
    22501 tttataaaat attatagaga aagagtgatt gttttgagca aaagagaatt gtttgcaatg
    22561 ttgccttcag ttgatgaagt attaagtgat aatagaattg tagaaataat aaatgaatat
    22621 cctagaagtt tagttttaga atctgtgaga gaagtaatag atttaaaaag acaatttata
    22681 ttaagattaa aagaagatgc atcaaactct gtaacaatag agtttggtga aataatagaa
    22741 tctgcaatag agagagttaa gttaaattac tcattatctt taaaaaaagt tataaatgca
    22801 actggtacag ttattcatac aaatttagga aggtctttac ttagtgaaga tataaaagat
    22861 gaattatggt gtgctgcttc aaggtattca aacttagaat acgatttgga taatggagaa
    22921 agaggttcaa gatactctca tttaactagt acaataaaaa gacttactgg agcagaagat
    22981 gtactagttg taaataataa tgcagcggca gtacttcttg tacttagcac aatggctaaa
    23041 ggaggagaag caattgtatc tcgtggagaa ttagttgaag taggtggttc atttaggatt
    23101 ccaagtataa tggctcttag tggtgcagaa ttagttgaag ttggttcaac taataaaact
    23161 catttgaaag attacaagga ggccattaca gaagatacaa atgtattgat gaaggttcat
    23221 acaagtaatt atagaataat gggattcaca gagagtgttt ctatagaaga attggttaat
    23281 ttaggtaaaa aatataaact acctgtaata gaagacttag gaagtggagt ttttatagat
    23341 ttatcaaaat atggtttatc ttatgaacca actgtattgg attctattcg acaaggtgca
    23401 gatgtagtaa cttttagtgg agataagatg cttggaggtc cgcaagcagg tattatagtt
    23461 gggaaaaaag agtatataga aaaaatgaag aaaaatcaac ttactagagc acttagagtt
    23521 gataagctta caatatgtgc tcttgaagca acattgagaa tgtatttaga tgagacaaaa
    23581 gctatagaaa atatacctac tttaaaaatg ttgacatata aaattgaaga attagaagta
    23641 aaagcaaata aactatttga aaaaataact gctttaaact taaatgcaaa cataaatata
    23701 gaagatggat tttcacaggt gggaggaggg tctatgcctc ttgaaactat aagtacaaag
    23761 gtaatatcaa taactcctga acatatgaat gtttcatctt tagaaaaaaa actaagactt
    23821 agtgaagcac atataatagc cagagtgtat gataataaat atgtactaga tgtaagaact
    23881 atatttgatg atgaatttga tgtaatagtt gaggaattga gaaaagcatt taattaaata
    23941 aatttgagtt aataaatatt aaatttagaa gtataagtat gaagttttga ttaaaaaata
    24001 gctacacaca cttgtgactt tagtcatagc aggtttagag ggggcaaaaa atgaaaaatg
    24061 tagtaattgg aacagcaggt catattgacc acggaaaaac tactcttata aaagcattga
    24121 ctggaagaga gactgatacg ttagatgaag aaaaaaagag aggtatttca ataaacttag
    24181 gatttactta ttttgattta ccaaacaata gaagagctgg aattgttgat gtcccaggac
    24241 acgaaaaatt tataaaaaac atgttagctg gagcatcagg aattgatata gttttacttg
    24301 ttgtagcagc agatgaaggg gttatgccac agacagttga gcatttagat attctaagct
    24361 ttttaaatat caaaaatgga atagtggttc taactaagtc tgatactgta gatgatgaat
    24421 ttagagagct agtaaaagaa gatatttgtg aaaaaataaa aggaactttt cttgaagatg
    24481 ctgaaataat tgaagttgat tctatatcta aaagaggtat agacactctt attgaaaaaa
    24541 tagataaaat gagtgatgat atagaagata aaaatgaaaa ctctccagca agacttaata
    24601 tagacagagt attttcaata aaagggtttg gtactgtaat aacaggcact ttaatagaag
    24661 gaaagataag tatagaagat gatttggtta tatatccaca ggtattaaaa acaaaaataa
    24721 gaagtataca agttcatgga gaaaataaag atacagctta tgcaggtcaa agaacagcaa
    24781 tcaatatatc aaatgtaaaa gtggatgata taaaaagagg agatgtttta gctgcaccaa
    24841 actcattgga ggaatcaatg atgttagatg taaaattatc attggtaaat catttagata
    24901 aaggattaaa gcactgggat agattaagat tgtatcatgg aactagagag atactttgta
    24961 gagcagttcc actagataaa gaattaattg aaagtggaga aagtggctat gttcagttga
    25021 gacttgaaga aagcatagta tctaagaaag gtgatacgtt tgtagtgaga cgttattctc
    25081 caatggaaac tataggtgga ggtgtaataa ttgacccatc tcctaaaaaa cataagaaat
    25141 ttgatgaaaa agttatagaa gcattaaaaa taaaagaaaa aggcgaacta aaagatataa
    25201 tagaagagta cttaaaaagg aatttaaaaa attatccaaa tataaaagaa ataatgagct
    25261 atagtggtgc tcatgaagaa gatgtaaaaa gagcgcttga aacacttata tcagaagata
    25321 aagtatttat tataggaaat atgtatatgc atataaatca atacaataaa ttaaaagaaa
    25381 atacaataaa gcttttaagt gagtaccata agaagtatag gcttagaaaa gggatactaa
    25441 aagaagaagt aaggtctaag atagagagta attttaagac tagggagatg gatattttat
    25501 tagaaaaatt gtctacagaa aatgctataa aaatagaaaa taatatagta tctctattag
    25561 attttgaggt tattttaaat gataagcaaa aagaaatagc aaaaaaaata gaaaaaagat
    25621 taaaatcatg tggagtttct tctatactga caatagatga agtttctgaa ggaaatcata
    25681 actatgctga agtattggag tcaatgatag gtaataaagt agagaaatta gatgatttgt
    25741 atataatgga caaagatatt tatgaaaatg caaagaacat tttgataaat tatataaaag
    25801 aaaataaaga aataacatta ggagaataca gagatttgat tgattctagc agaaagaatt
    25861 gcatgattat attggaaaac ttcgatagaa acaaaataac taaaagagta gaaaacaaaa
    25921 gaattttgtt ttagcagaaa atgtctgttt ttgctttaag gtataaagaa ggaattataa
    25981 tgtcgagtat cgtataatta attagtataa aatattaatt aagaggaagt gtaaagtatt
    26041 gaagaatata atacctgatg taaaaccaga tataaaagaa agagaagaca tgagcctttt
    26101 atttataata atgggctcag tacttttatt atttattata aaagatatag agaaatatga
    26161 aactttatta catgccatat atacgatatt tttagtagct acaggagtaa ttatatttaa
    26221 tactacaaaa tttaaagtag attcattttc tacattttta ggattaattt ttatagctac
    26281 tggagtattg gagtgtatat atttatttaa ttccttgggg attaaaacta agtctataat
    26341 ggagattaac atcacaatat cagcaataac agatttattt cctatactgg gtgtatactt
    26401 aagttttaaa tttgttaagg ataacaaaca aatttattca agtgtaattt tatttatagc
    26461 aactgctaca ttaacaataa catctttatt cataatatca ggaatttgtg actatctggg
    26521 aaatggtaaa tcacaatatg ctttaggagt tataatatct atattcataa taattatttc
    26581 tatcattagt ggaattgaat taaattcaag ttcaaataaa ggtaaatggg aatatgatga
    26641 aaaaaagatt tttaacagaa taatagtaat aataatctta tcaagagttc caaatctatt
    26701 gcatatagtt attgataata gaaatgtaga gaggatatta tcacaaataa taataaatgt
    26761 agccttatat tatttatata attacatagt atctaagaat attaagaaaa cagcactgga
    26821 attgcatgat acgaatgaag aattaacaaa aaaaacggaa agtttaaaag aaaagaataa
    26881 aaaattaatc tatgagacag aaaagataga ggaacttaag ggaatactta tgaaaagaga
    26941 atctaggcta caatctacat tagatgtagc agtaaatagc atagtagtat ttagcaaaga
    27001 tggagaaatt acatatgcaa ataagagctt tagaaatact tttggagact ataaagaaca
    27061 agataaattg actgataaaa taaaaaactt caatgatttg atagaaagca ttcattatgt
    27121 gtttgttcat gagaagaatg cagagaaact tattagtaca agcgataata aagtttatca
    27181 agctatattt gcacctttaa ttattgcttc acaaacagaa ggagtattgt gtgtactaga
    27241 agataagact aagaaaaagg agtacgaacg caaacttatt gaagcaaata agagaagtaa
    27301 agatttttta gaaagtattg gagatggcat agtagtgctt gaaggtaata acaaaatata
    27361 tgttaataat gcatgtaggg aaatttttaa aaataattta gagagtattg atttttcatt
    27421 agtatgtaaa agtgaagaaa gtatagaaaa aagatatgta atagatggtg aagtaaagta
    27481 tgtagagatg agtttttctc agtatactaa tgctggtact aataaaacta ttattgtaat
    27541 acgtgataca acctctcgaa aaatatctca aataaaactt gaggaaagtc aatcatcata
    27601 ttcaagattt atagatatat tacctgatgg catatgctta ttaaaagaag atttaagtat
    27661 aaactatgca aataagtcgt ttttagatat gctagctttt acaaacatag atgatataaa
    27721 agattctaat attaaactat taatgaatgc gaatactgaa gaaaaaatga aatttacgga
    27781 taatatggct aaagttttaa atgaaaatag aagtatactt ttactggaac atgaattgat
    27841 aaatagttat gatgatatag tagaagtaga attaagtgca ttaccttttg ctatatacaa
    27901 tactagatac ataatgctta ttataaaaga tttagtacat aagaaatatt ccgagcaagc
    27961 agaaaaagaa ctgcttgaaa gatttaaaac agataaaatc aaaacggagt tttttgcaaa
    28021 tatgtctcat gaactaaaaa ctccactaaa tgttatatct agttcaaatc aactggtaga
    28081 ttctttctat agaaatgaaa agataaaaga ttacaataac aatattaaat ctcatgtgga
    28141 tttagttaga caaagttctt ataggcttca aagattaata aataacatta tagatttaac
    28201 aaagatggag tctgggttct atacactaaa acttgcgaaa tacaatattg tatctgtaat
    28261 agaagactta tttatgaata tagaagagta tgcacttaga aaagacataa aaattctttt
    28321 tgatacagac cttgaagaaa ttaatgttta tatagacaag gttgaaattg agagaattat
    28381 gttgaatcta ttgtcaaact gtattaagtt tactgataat ggaggctgga tatatgtaag
    28441 tattcattat aaaatagata aagtaatcat aagtgtaaaa gacactggag ttggaatacc
    28501 acaagataaa ttagagttga tttttgaaga attttcacaa gttgataaga ctttatccag
    28561 aaatactgaa ggaagtggaa tagggcttgc tatagttaag aatctagtaa gtcttcatgg
    28621 tggagatata gaagtagtaa gtgaagtgaa taaaggcaca gagttcttaa tttcacttcc
    28681 aatgaaggga tttagtagtg aacattacac agaagataag agaatatata atattcaaga
    28741 aaaaataaaa atagaatttt ctgatatata ttattaaaaa tgattatgag caaaaataaa
    28801 ataaaaatac tatttgttta aatatttaag ttgtaagaaa taggctatca taaggtaaat
    28861 tattatgata gcctattttt agctaggttt tatatctata tgtgagaaca tataatctct
    28921 aaattacctt caaaactaaa atctccaaag tcgtttggca gtataaaatg catacccttt
    28981 tttaaattat attctacaga attgtgtata agttttccat ttccatccaa cacactgtat
    29041 aaagaaaaag gtgtattgtg tgtaaaatta cattttccaa aaacatctaa tttataaaca
    29101 ctaaaaaatt cgtttgacac aaatgttgtg catttaaagt ctgatgttga tacgatttta
    29161 tagtcagtat cgaaatttat atgaggaaca ttcgtaacat ctattgattt ttgaacatga
    29221 agttcacgct tattacctga attgtcagtt ctatcataat catagactct ataagtagta
    29281 tctgagtttt gttgagtttc taaaatcaat gttcctttgc atatagcatg tatagtgcca
    29341 cttggaacat agaaaaagtc tccctttttt atattaacct ttctaagtag tttgtcccat
    29401 tcattattat ttaccatatt tacaaattct ttatgagatt ttgcattatg acctattata
    29461 atttctgcat catcagaaca atctattaca taccaacact ctgtcttacc taactctcca
    29521 ttttcatttt ttttagcata ttcatcattt ggatgaactt gaactgataa attatcattt
    29581 gcatccaata tttttgtaag taaaggaaac ttatcaccag gtgtgtttcc aaataattct
    29641 ctatttttat tccacaattc agatagtttt ttacctttat attttccatt ttctattaag
    29701 caatctccat ttttatgaga gcttatagcc caacattcac cagttgttgg cgaatctatt
    29761 tcataattaa atttatcttt aagtgcagta ccaccccata ttctatccat aaaaataggt
    29821 tttaaaaata atggttccat aatcctgttc ctcctatgat attaaattta tcctacaaat
    29881 aattat
//
LOCUS       DAELTA010000032        29650 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.032, whole genome shotgun sequence.
ACCESSION   DAELTA010000032 DAELTA010000000
VERSION     DAELTA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.032"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(90..1766)
                     /gene="bclA2"
                     /locus_tag="KPG13_001242"
     CDS             complement(90..1766)
                     /gene="bclA2"
                     /locus_tag="KPG13_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG0984979.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2144..2683)
                     /gene="hpt"
                     /locus_tag="KPG13_001243"
     CDS             complement(2144..2683)
                     /gene="hpt"
                     /locus_tag="KPG13_001243"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG0984980.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3011..4318)
                     /locus_tag="KPG13_001244"
     CDS             complement(3011..4318)
                     /locus_tag="KPG13_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG0984981.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4743..6518)
                     /locus_tag="KPG13_001245"
     CDS             complement(4743..6518)
                     /locus_tag="KPG13_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0984982.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6515..6652)
                     /locus_tag="KPG13_001246"
     CDS             complement(6515..6652)
                     /locus_tag="KPG13_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984983.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6730..7299)
                     /locus_tag="KPG13_001247"
     CDS             complement(6730..7299)
                     /locus_tag="KPG13_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0984984.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7483..8118)
                     /locus_tag="KPG13_001248"
     CDS             complement(7483..8118)
                     /locus_tag="KPG13_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0984985.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8786..9667)
                     /locus_tag="KPG13_001249"
     CDS             complement(8786..9667)
                     /locus_tag="KPG13_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0984986.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9787..10794)
                     /locus_tag="KPG13_001250"
     CDS             complement(9787..10794)
                     /locus_tag="KPG13_001250"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG0984987.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10814..11296)
                     /locus_tag="KPG13_001251"
     CDS             complement(10814..11296)
                     /locus_tag="KPG13_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0984988.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11314..11781)
                     /locus_tag="KPG13_001252"
     CDS             complement(11314..11781)
                     /locus_tag="KPG13_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG0984989.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11796..12554)
                     /gene="prdD"
                     /locus_tag="KPG13_001253"
     CDS             complement(11796..12554)
                     /gene="prdD"
                     /locus_tag="KPG13_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG0984990.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12634..13359)
                     /gene="prdB"
                     /locus_tag="KPG13_001254"
     CDS             complement(12634..13359)
                     /gene="prdB"
                     /locus_tag="KPG13_001254"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12907..12909),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG0984991.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13397..13681)
                     /locus_tag="KPG13_001255"
     CDS             complement(13397..13681)
                     /locus_tag="KPG13_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0984992.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13762..15642)
                     /gene="prdA"
                     /locus_tag="KPG13_001256"
     CDS             complement(13762..15642)
                     /gene="prdA"
                     /locus_tag="KPG13_001256"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG0984993.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(16071..17831)
                     /gene="prdR"
                     /locus_tag="KPG13_001257"
     CDS             complement(16071..17831)
                     /gene="prdR"
                     /locus_tag="KPG13_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG0984994.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(18238..20412)
                     /locus_tag="KPG13_001258"
     CDS             complement(18238..20412)
                     /locus_tag="KPG13_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG0984995.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     regulatory      complement(20994..21079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(21484..22797)
                     /gene="prdC"
                     /locus_tag="KPG13_001259"
     CDS             complement(21484..22797)
                     /gene="prdC"
                     /locus_tag="KPG13_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG0984996.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(23186..23950)
                     /locus_tag="KPG13_001260"
     CDS             complement(23186..23950)
                     /locus_tag="KPG13_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0984997.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            24241..24444
                     /locus_tag="KPG13_001261"
     CDS             24241..24444
                     /locus_tag="KPG13_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG0984998.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            24635..25171
                     /locus_tag="KPG13_001262"
     CDS             24635..25171
                     /locus_tag="KPG13_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG0984999.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(25175..26041)
                     /locus_tag="KPG13_001263"
     CDS             complement(25175..26041)
                     /locus_tag="KPG13_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG0985000.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            26189..26434
                     /locus_tag="KPG13_001264"
     CDS             26189..26434
                     /locus_tag="KPG13_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0985001.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(26548..27846)
                     /locus_tag="KPG13_001265"
     CDS             complement(26548..27846)
                     /locus_tag="KPG13_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG0985002.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(27979..29010)
                     /locus_tag="KPG13_001266"
     CDS             complement(27979..29010)
                     /locus_tag="KPG13_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0985003.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
BASE COUNT         8845 a         5558 c         3404 g        11843 t
ORIGIN      
        1 ttcatcatat aaacctatta aatattattc tatttattgg tatatgaaat catattgttt
       61 attattttat aatcttttca tcatataaac tattgtattc tataaactga tacatatcca
      121 gctgcagaag tacctgtcgt gcctgaacca aatgtaaaac ttaaagtaac agctgctgct
      181 ctagcatcaa ctaaaaagct taaacttaca cttacatctg atgaagtaag tgtatttact
      241 gcttttgcaa taaagttatt ttgagctact ccattaaagt tagtagtaat tgacataaat
      301 cctgcaagtg aaagtgtggc acgtataccg aaagatacat tgtatatccc tccaactgca
      361 agatttatag tactacttgt tggaaaagtt atattagttc catttataaa tgatgaatta
      421 aatgtcacta atgtattatt cactaaactt gaagaggaca ctgtaaattg tgcattgttt
      481 gctgctaaca ctcctgttgc tccggtcgct ccggttggac ctgttgctcc atttgctcct
      541 gttgctccgg tcgcccctgt tgctcctgtc gctccatttg ctccagttgg gcctgttggt
      601 cctatcgctc ctgctactcc atttgctccc gttgctccta ctgctccggt tgggcctgtt
      661 gctccatttg cccctgttgc tcctgtcgct cctgttgctc ctgctactcc atttgctccg
      721 gttggacctg ttggacctat tggacctgct actccattcg ctccggttgc tcctactgct
      781 cccgttgggc ctgttgctcc atttgcccct gttgctcctg tcgctccggt tggacctact
      841 gctccatctg ctcccgttgg gcctgttgct cctactgctc ctgttggacc tattgcccca
      901 gctactcctg ttgctcctgt cgctcctgtt ggacctacca aaccatttgc tccagttgct
      961 ccggttgggc ctgttgggcc taccaaacca tttgctccag ttgctccggt tgggcctgtt
     1021 gggcctgttg gacctaccaa accatctgga cctgttgctc ctactgctcc tgttggacct
     1081 acctctccat ctgctcctgt tgctccggtt gctcctattg ggcctgttgc cccagttgtt
     1141 cctgttgctc ctgtcgctcc tatacttccg gttggacctg ttgctcctgt atttcctgtt
     1201 gggcctgtag cacctgttac tccatttgct ccggttgctc ctgtatttcc tgttggacct
     1261 gttattccat ttgctccggt tgctccggtt gctccagtta gtccggttgg acctgttgct
     1321 ccagttgctc ctgtattccc tgttggacct gttattccat ttgctccagt cgctcctgta
     1381 tttcctgttg gtcctgtaga gcctgttatt ccatttgctc cagttgctcc tttgtttcct
     1441 gttggacctg ttattccatt tgctccggtt gctcctgtat tccctgtcgg acctgttatt
     1501 ccattcgctc cagtcgctcc tgtatttcct gttggtcctg tagagcctgt tgttccattt
     1561 gctccagtcg ctcccatatt tcctgttggt cccgttattc cattcgcccc ggttgctccc
     1621 gtatttcctg ttggacctgt tattccattt gctccggtcg ctccagttgc acctctaggc
     1681 cccgtcggtc ctgttggacc agtagcacct gttggccctg ttggacctac atcttgatat
     1741 aaacttggac ctgaaatatc actcatataa ttaatcctcc ttttttaaag ttagagtatt
     1801 actctataaa tattgagtat tactctatat aattatatga atcgacaatt tcttatgtga
     1861 taatgtatct atttgcttat atcaacatat atgctttgat tcttgaattt cattactttt
     1921 ttgtaaaatc ttttacaatt attagtactc ttttacaatt attaatacaa ttaaaaaact
     1981 cattttccat caattattta aaattttttt attatgttat aaaaaataga taattaaatt
     2041 gcataaaatt aatttaatac tactttaatt actttgtaaa taaaaaaacc tcaacacgat
     2101 taataaaaat catattaagg catccacaaa attatataat tgcttatcta tcttcatcag
     2161 taacattgaa tatataagga acatttctat aataatcacc aaaatcaaac ccatatccaa
     2221 ccacaaactt atcttctatt tcaaatccac aataatctgg cactaaatcg acttttcttc
     2281 tgcttggttt atccaaaaga acacaacatt ttaaactagc aggattttta gcttttaaat
     2341 gttttaacac aaaatccata gtaagtgcag aatcagtaat atcgtctaca accaaaacat
     2401 catatcctgc tatatcagta gtcacatctg aaactacttt aacccttcca gtactttctt
     2461 cattgtttcc ataactagat gtagtcataa aattaactct aagtggtaaa tttatatttc
     2521 taactaagtc tgcacaaaat ataaagctac cttttaatag agaaactacc ataagatttt
     2581 tatctttata atctttttct ataattgctc ctaattccct taatttatct ttaatttttt
     2641 cttctgaata caatacttcc catttttttg tttctatatc cataaaaaga cctccaattg
     2701 taataatcct tcttaatttt ataagagatt gctcactttt tcaatactct tattcaatat
     2761 tagtcaatat tattgagttt ttatgttcat tcaacaatta ttaacaatgt atcttatatt
     2821 ttaagtagtt catatatcca cactaaatta catactatat attttagaca aaaaaagaaa
     2881 aaacttgcac taacaagttt tttctattat acataaattt atacataatc atattttatt
     2941 agagggtact aattaaattt atttatgcta tttttgttat acctcataat acaaaacata
     3001 ttataaagta ctagtcaact ttattcatat cattcattac atttaagatt atctcatctg
     3061 ttatagacat acctttgtca cttactctac ctaagttttg tatactttgc tcaacacttg
     3121 aaccaactat accatttagt ggtggtacaa aacaatcttg cttagctatt atagcacttt
     3181 gtatagctgc agatgctgca gaagataatt ttaatgcaca accagctttc gcaccgtcac
     3241 atatcattcc acttaagtca gctatcatat tttctatagc tccttctatt tgcttttcag
     3301 taccattcat aagccaagag ataccagcag ttgctccagt agaagcagca acaccacatc
     3361 cacatacagc agataatctt cctgtatagt tcttaatata accagttact agatgagata
     3421 tagctaaagc ttttgctagt ctttcatcag attgtgggaa ttttttatta taagcaacga
     3481 ttggaagaat tgctgttaat ccatgatttc cacttccatt tgaactcata acaggcattt
     3541 ttacccctgc cattcttgca tctgaagctc cagcagttaa catcattgca taatttataa
     3601 catcattacc taaaagacct tcttctatag actttttaat tccatacccg acaccaatac
     3661 cagttttttg ttttaatcca tattcagcca tctcttcatt catttttact ccatctagta
     3721 aaaattctat atctttaaaa tcaatttctt ctacattttt tattaattct tgtatagtta
     3781 ctgtatccat taaactttca gctttttctg ccgcatcatt tgaagctgat acttttggtt
     3841 cattgtctaa taatacttct ccatcttttt ctaagaacgt aaaattatca tgtttagttc
     3901 ttattctcac tttagcaata tgattttttc cttttaacac aacttctata aatactttct
     3961 ctcttgtatc agcaggtgtt attgatagtg gagtattatc catataatcc tcagctattt
     4021 taacttcctc ttttgttaga gtttctaaca cacttaatcc attttcagaa tgtccaccta
     4081 ctatacctag agctgctgca atttttaatc ctaatctttc agtacctggt atacctacac
     4141 acataccatt tttatagata ctaggactaa ctaacatacg attttcaact atttcttctc
     4201 ctaataattc ctttgccttt gcacatgcta gagctaaggc tactggttca gtacacccta
     4261 ctgctggttt aacttctgcc tttaacattt ccactaattc ttttcttata tctctcatta
     4321 tatataaacc tcccaattaa gtttagattt agtactcaaa taattagtta aataatttct
     4381 tatctatatt tatagcaaaa tacatgccat attttttata tctttttata tttttagtct
     4441 tatattctat aaccattggt atgactgcct ctatttatgt atttttttta tttgtataga
     4501 gtatttatat tctttaaaat ggagaaacat atgacaattt cgcaaaaaga caaaaaaaag
     4561 tctcaaaatg agattctata tcattttgag acggctcaaa tatattattt tttaacttat
     4621 aaaaaccatt ttttaattca taaaaaaaga ttctgcaaaa cacttatagt ataagtttaa
     4681 agctttcttt aaagttatga atttagaaaa atttaaattt tatatagctt tttattatat
     4741 tactatgaaa ttatattgta tttttcaatt tttctatata atgtagctct tgatattcca
     4801 agtgctttag ctgttaattc tttatctttt ttataatctt tatacttatt taaagctttt
     4861 attatctcaa tcttctctag ttcatcaagt ggaattatcc tatcgctttt aacttctaac
     4921 tcttctacag aggtattctt aattttttga ggaattacat tcattgtgat tattgaagaa
     4981 gaagacatat tcacactata ttcaataata ttctgcaact ctcttatatt ccctggccac
     5041 ttatattcca tcatagcatc aacaaccctg ctatctatgt caataacatt tttatctaat
     5101 ttttttgaat actctttaac catatgcttg attataagtg ggatatcttc ttttctctct
     5161 cttaaacttg gtaattttat aggtataaca tttaatctat aatataaatc ttctctaaaa
     5221 gtgccatttt gaaccattct ttctaaatct ttatttgtag cagcaataat tcttacatct
     5281 attgaaacat ttgatttact tccaatttta ttaagttcct tttcttgtaa tactctcaat
     5341 aactttgact gtaaatgcaa actcatatct cctatttcat ctaaaaatat acttccctta
     5401 tgagctactt caaacattcc aagctttcca ccttttttag ctccagtaaa agctccctct
     5461 tcatatccaa ataattcact ctctaataaa ttatccggta tagcagcaca gtttacagct
     5521 ataaaaggat tatctactct attgctatgg ttgtgtatgg ctcttgcaaa tagctcctta
     5581 cccgttccac tttccccagt aataagtact gttgatgttg atttagaagc tattagggct
     5641 tcctttttag aatttcttat agctacactt tctccaatta tgttttccaa ttttattcta
     5701 taatctttat tcatcttatt gtagtttttt atagcatctc ttttatctat aaaatcaata
     5761 acatatccct ttaactcatt ttttaatact attttattga tgttgtatat tcccttcgaa
     5821 tcatatccgt atcttttata aaaaaaacta caggatttgt ccttcttaaa gttatttata
     5881 gaaggtttct ttataaaatc taactcgaca aatatgtttt tgccagttat actatctttt
     5941 atattgaaaa gctctttaaa ttttgaatta tatttatcaa tttttccatc cacatcaata
     6001 gacacaacag ctttgtccat attgtttaat agtgtctcta attttttctt ttctaattca
     6061 agctcataag ttttaatttg tgcttttaat ttattagata ttaggtctgc cattttgcct
     6121 aaaaagttta taagaccact tttattattt tctataattt gtgcttgttc ttttgtaaag
     6181 gctataaggc ctataacacc atgagaatcg ccatcacata taataggaca acacacctct
     6241 gcaaactcta cacaagcccc acttttatag cattccctac atatatcact actctttggg
     6301 tcatcaatta gtattgtaat ctgttctgta agtgattttt taaaagccgt atatttatct
     6361 actttctcac ctattttgtc tatatatact cctgtacctg caatcctaaa taactcctta
     6421 tttactatag ttacatctat attgagtact gatactatag cttcagctat attttgaata
     6481 tcagatttta tatattctaa acctatcata atcatcattc cttatttttc tttagcataa
     6541 gtatttgtct tatttcttta agaatcaagt atatcttatt taaagtaaat tggattgaca
     6601 tcaggattac aaagagcata acaattatta ttaactcata atttatttcc atctaaacat
     6661 ctccttataa caaaagacta ggcttaacct agtcttttat attatatcca tatttatatt
     6721 ttaactcatt tatcttcttt ttcccacata aatatatttt aatttatttg actctttaat
     6781 cataccatca aagtttaatc ttgaaaatat attaaatact tcatgtggat agtgaatctt
     6841 tgcaacagaa ccacaaaatt tatgtttaag atagtttaac ttaactttta aattttttaa
     6901 atttaaatcc tctaatataa cctctccatt gccctttaac actctttgta tttcagatga
     6961 agctttttca caatctgtaa taacattaaa ttggtcaact attattatct tgttgaactg
     7021 attatcatca aatggtagaa actctaactt acctttaact acattgcaat tttcattata
     7081 cactagagaa taacagacat cttcataatt actattctcc agtatagtta cagaagttcc
     7141 aagacctctc aatctcttgc ctaggtttcc tatatgacca acaagcaata ttttatcacc
     7201 tacattatac tcaattgtat ccaaaaaaca ttctaattca tcagatgtat atttagcttt
     7261 aactggctgt ttatttatct gacataatat gcttttcaca atgcttcacc tcttcatttc
     7321 ctcaattata aattatgtac agattacatt ataataaatg gcgagcaact attcaagaga
     7381 tatttcatta gtattttttt aaacaatctt tatacatttt aatatgatat tataccaaat
     7441 ttattaggat tttattcata aaaatcagtc tattttttat gtttattcat tgttattttc
     7501 atccaatttt tttattttcg atatggatac ttcatatgcc atttttttga ccacattatt
     7561 ttcatctatt tttttctcat attctctact ttgaacccta ccccaaagct gtattctatc
     7621 accaaccttt agattctttg caaatttagc atttctaccc caaactattg atggtatata
     7681 atcagattta ttataaggtc tgtttatagc aactaataaa tcggttattt cccttcctaa
     7741 aggtgtcttc ctatacacag gttccttaca tacatatcca tctaaaaata tactattagg
     7801 gtctttattt tcctcgtcta tactttttat ttctctgaca aacactgtaa gcactaatct
     7861 atttttatta tttaatgttt tgttatatga tcttaattgg cctacgacat tgactaaatt
     7921 ttgtttatca aggtcaatat cttggagaag tctttctgat acagtcattg gaagtatatc
     7981 aaaagaatcg cttagtctgt ttatttttat tttcatatta taaaattttt ctccaaatat
     8041 ctcatggcta aattctagct tattgtctaa ctcccctctt aagtttacta cgttattctc
     8101 atctttaaca gctatcatat tttatccctc cacaattgtt aattcttata tttctggtat
     8161 cagcttatat aataaatata tttataaggg atgtatttta ttacaaatct ttgattgctg
     8221 tttaaaagtt tttatttagt tataaatttc atttaatatt tcacttttat aattataaaa
     8281 ttattatctt tttgtaccac atatactaat taaactacct atttcagttt tgtattaatt
     8341 ataaaaatcc tattatataa ttaataagtc atatataact taaatttatt cactctatat
     8401 aagcaacttc aacgaattaa atttattgaa ataattcaga tgaatattga gtcattataa
     8461 acttattttt cttaattata agttatatta gacttattac aggttatatt tttattaatc
     8521 tgtattaata tatgaacatt tattgaactt attgattata aaatttaaat atttagcttt
     8581 aaattatgct ttattcttta aaaatacaac gttttttaga ttaaatctac taaatcacta
     8641 tatatattac ataattggca tgttaattgc tttatattaa tttagtaaat aaaaaactgc
     8701 cctaaaatat taattcaata ttttaaagca gtttataatc tttattatta gctgtaatat
     8761 ttaataaaca ctaagtagtc tttaactaag cagtcttttt gtttttattt gcagccatta
     8821 tcattgtagc agaagtataa gctataacta ctattactaa tgcttttaat atatccattg
     8881 gtagagtttt tactatatat gcagctacga aaactcctat taaaccacat atagctatta
     8941 taactgatgc ttttttagta tatgcatctt cttttatgaa tttcatactt gcaactggca
     9001 ttaataatgc acaagaaccc ataactattg ggaatgcaac ttttgcagac attcctaaga
     9061 aataaaccat tgccatacct ggagcatata gacctatacc agctgtcatt aaagctccta
     9121 gtataaagtt acctattaca cctattacta atttagcacc agtaagacct aattcatttc
     9181 ctccacctgg taatacgtta atccaaggat gacctaataa cattaatatt gctgttaatg
     9241 ctaaagttac acccataaca agttgtattt ttctctcgtc catcttagct attattcctg
     9301 cacctatata tgaaccaact gctgctgcca caaccataga tactaaagta acaccatcta
     9361 ctgggataat tgatgtaaat atgaatgctt ctgtaacaac tggaatagta tgtgctacgt
     9421 ttaatgtacc aggcattttt ttatctggta tatttaactt taaagccttt gacattgcta
     9481 ttgttggagc aaatgaccct attcctaatg tatcaaagaa gtctgttata aatcctgtta
     9541 cagcaacttt tggagtactt tgttcttcta gtttcccctc tttgtttgct ttagctacgt
     9601 ctttaccaaa cacaactgca aagattgctg cgaatactac atataatgcc tttaagattg
     9661 ctaccataat ccatcctcct atttttttat tttttcatat attctgaatc ttatagccta
     9721 aaagctatag aatagcaacc gacaagtggc tactattcta ttttaatttc ctaatttatt
     9781 tattacttat ttaagaataa atccatgttt aagtgggtct tcttcatcta ttacaaaatg
     9841 attaaatcca gttatataag cagaaccagt tattttaggt actacagcat tgaaatctgc
     9901 aactttagtt tcttcaacta tttcaccttt gaataaagtt cctaatatac tttcatatac
     9961 gaatttttct cctactttta attcaccttt agcatgtaaa gtagctaatt tagcacttgt
    10021 tccagttcca catggagatc tgtcaacttg accttgacca aatataacta catttttgta
    10081 agtagcttct ggatgagttg gctcatcata tatttcaact aagtctacag tttttatatg
    10141 agctaaagtt ggatgttgta tttctatttt ctcatttatt atatctctaa gtttcatagc
    10201 tagttcagtt aatttaccag cattttgagg ttcgattttt aaacctaatt gacttgcatg
    10261 tattatagca aagaagcttc caccaaatga tatgtcaaat ttaacagttc caacacctgg
    10321 taagtcaact tcaacacctt ctttatataa gaaagctggt acgtttaaga atgatacttc
    10381 tttagctttt ccatctacaa ctgtaacgtc tcctcttatt attcctgctg gagcttccat
    10441 aactacatga gttacaggct ctactgctgg aactacacct gtttctatag ctgctgtcat
    10501 agctcctatt gtaccatggc cacacatgtt aaggtatccg ccaccatcca tgaagattat
    10561 tccaaagtca gcatctgggc aacaaggttg agtcattaca gaaccaaaca tatcattatg
    10621 tcctcttggc tctaacatta tagcagttct taaataatct agattttcct ctaaatattc
    10681 ttttttctca ggcatagaat ttccttttat gttaggtatt ccacctacaa ctattctagt
    10741 cgcttcacct gctgtatgag agtctatagc ttgtatactt ctgctaaatt tcatatcaaa
    10801 ttccccctta ggtttatttt gcgtctttac gcatttttaa ttttaggaaa gccaatgctt
    10861 ttttaacatc aacttcatcc atgtgattgt ctccaactac ttctgtttct atgtagcctg
    10921 ttgtcttttt ataatcaatt atggtagctt tatccaaata tggattggta attactaatc
    10981 catctgctcc cataatagta agacctactg taggtatatc tgattttaca aactctgcta
    11041 tagcatttgt aaaatcaata tgctggtttc ctgccatatc taaagttata atcactccat
    11101 caggtcgcaa tgattcaatc catcttccaa gtctagttgc taaaaatgtt ttccattcat
    11161 tgtgctctga tgtcccaact acaaccactc ctaacaagtc tatgtcagaa tcattatcta
    11221 tcaaatctag taacgggtct ctaaatatat gaagtgatgt gtttttcatt gatgtagaaa
    11281 ttcccacaaa actcattccc ttacttaaat tttttatcca tgttttctca ttttaagttt
    11341 taaaaatgcc aaagcttttt tagcatctat ttcatttgtg ttgttttctc caactacttc
    11401 tgtctcaata ccttcttttg atttgttcat atcaactatg gtatccatat attgattgct
    11461 tacaacaaac ttagcttgag tcccattaaa tgtaactcca actacaggta tatctctttt
    11521 tcctatttcc tcaaatgtgt tagcgtagtc aacatgtgag tttccccaac catctgatga
    11581 aactatagct ccatctactc tcatggcttc cagccaagcg gccgttcttt gtcccacaaa
    11641 ggttttattt tcatttccat ctggagttcc tactactatt actccaagta aatctacatc
    11701 cttatcactt tcaactatgt ctaaaagtgg gtctctaaag tgatgaagag atgtttcttt
    11761 tgttgaaggt cctattccca taatatccac ctctcttaag tcatagctct aagggcacca
    11821 tctctgtatt cattaggaga tataatcata ggtacatttc ccatatctat aattgatgtt
    11881 cctccttcaa atccactagg ttcctttgag aataactgat tgtcatacat agcaccttga
    11941 cctgctattt gttttactat gacaaccttt tttgcatttg gtcgaatttt gtcaaaatat
    12001 tcacgaactt gagtagcatt tcttccgtct tccattttca aagacgctct tatttcttgt
    12061 ataaaatcat cacatgctct gaatgctgca agtggaagtg ttctttcaaa aggtaaacct
    12121 ccctttaaag ttacatctac atggattata taatcttcaa ctgatggagt tccatatctc
    12181 ccaaatacca tttggtcttt caaaattcct tctgaagaac caaattcatg catttgattt
    12241 ccatcttcat ctgcaccagt taacattaca tatacacccg ttaaagtatg tgtaataccc
    12301 tctcccagtc tgcctaaaac ttttgttgat attggtataa tgtccataat agtattaatt
    12361 tctctgtcat aatctccagg ttttataatg tctactttta tgtctgttat taactcttct
    12421 ctttctttta tttcatctat acttgataaa tctattgtaa gtacatcttt ctttatagaa
    12481 gtttttgaac cgaaattaac ttcattcata tgaaacggtt ttataacaag tcttctcatt
    12541 attttttctt ccataatatc cacctttgtt cttaaaaccc ctctcctaac cgtgtaggag
    12601 aagggttcta tattttcaaa tataaattac attttaaacg tgagctttat attcgtatgg
    12661 taatggaact atcttaccag cagatggtat tgcaactaat tgctctaatg tatctcttaa
    12721 tatatgtgtt tgcatttcta tattatgtgg ttcaccagca ttagcaccca ttggaactaa
    12781 tggagcaact gctctaggag ttccgttttg tctaactact ggaggaagag ctgctattat
    12841 tattgtaggt attccagctt cctctattgc tctctgcacg atcacggcag ttctatggca
    12901 agtacctcag ccagctgtca taactacacc gtctactccc tcttccttaa gtttagcagc
    12961 gatagcagga ccagtttctt cagtgaagac atgttggttt cctccaccac ccatgaatgc
    13021 atagtgcata ggagctactc ctttgataaa tccttctgca gctaattcat gtaatctatc
    13081 tatagggaac atacagttta tatctttgtt tacatctcca ttgtcatatc caccatgtga
    13141 taccatcatt tcatcaactg tagctgtatt aggtatagct ctaaatgtag tatctcctgc
    13201 tagattaaat ctcttgtcag attttaaatg aacaccagct gctgtagcta aagctatagt
    13261 catatctttt aattctttag ttacaggagt ccaaactgga ggaggtgtta ttggaacgaa
    13321 tatttcagat tgaagtcctt gtactgttgt aaggctcata tatttcctct cccttctatt
    13381 ttcaaacgat tatttattat ttttcttctt gttttgattg catttctgct tgtttttctt
    13441 tttctctcct aatattttct acatactctt gattaggctc tggacttaat acctcaggat
    13501 agcttagcat gaatccaact tcttgaccta tcttaagttc gtagtcagtt ataagcattc
    13561 tattaggtat tttaaattgt ccaagtctac cttttaggtc tatagtcact ccaccgcctt
    13621 tgatgtcaac tataatacct tcataaaaag tctctgatga gatatatttt aacctatcca
    13681 ttataattct acctctctat actgttttaa acttggatct aaatttatgt tattgtctaa
    13741 atcttagtta gtatctaaat tttagttttc gtctttttcg tatatttctc ttctcttctt
    13801 actcattggt agagattgct cattctcttc taatactatt ttttctcctg taactttttc
    13861 tatagcttct atgttattta atttaacatt tggattccat tttctttcag gagctttaac
    13921 ttctacacct tctatagcat ttttaagcat agccattatt ctaacagcat cttctggagc
    13981 taaagtgttg ttagataata tttcattctc tataccttgc ttagacttat tgttgtctac
    14041 catgtgagtc atgtatttat taccaacaac tagagcacct tgaacagctg agaaacttac
    14101 accaactact ggtatacctc tcatacctat ttgctctatg tgagatgcga aatctatatg
    14161 gttgtttccg aaaccttcag tagttactac agctccatca acttccatag cttcaactaa
    14221 catacctaat ctcttagata catagaattt ctcagcattt acttgtggag aaccaacaaa
    14281 tacaacacct actaaatcta tttcttcgtc ttccatagct cttattacta atggctctct
    14341 ccagtaatgt cttgacattt cttttgatgc aggtcctata caagttaatg catggatacc
    14401 accatctaat acttcaagtg gagataatac aactggcatg tttcctaagt caacgtttgg
    14461 ttgagctcct aatgtaccaa ctggctcaac tggtaatatt aggttatcat gcattgcacc
    14521 ttgtcccatt atttctttta taacaacaac tttcttttta ccttctcttc tgtattgctc
    14581 tattacttct tcatcaacta ctaaagagtt gtcaactttc tttaatgctt ctcttatttc
    14641 ttgagttaca tagtcaaatg cacggtgagc agctaaaggt cctggtcttt ccatgttagt
    14701 tcctgctttt attgttactt gacctttgat gaatatttca cctttgtcag cagcacctgg
    14761 tctaccccac attatagtag tatttaactc accttctgaa gaaccaaatt cacctatttg
    14821 aactccatct tcatctgtac cagttaatac cattacagct ccatctataa ctctagttat
    14881 acctgaacct aattcgcctt cttctttagt agcgattggt tgtatatcta atacagcttc
    14941 actgtaagtt tcatatttat caggagttat tatttctaac ttcatatcta caactaactc
    15001 ttgagtttca ttagcttctt tacatatttc ttctgcgttt ctgatgtata aagtagttcc
    15061 ttctatttta gtttcatcag caaattcaac tttatctatt ttaaagtgtt ttttagttaa
    15121 gcttcttaaa agtttctttt cttcaacttt tggagctact tctgctgctg cacttgccac
    15181 tggagcagca acgtttgcta ctggagcagc aactcctgct tgaccagcta ctgataaagg
    15241 tatctctaag cttatgttct taccttcacc tatgtgtatt ttaactgttt cacctgcaac
    15301 tcctgtgctt actggaacta ctgctgcttc tgatactgga gctgcttctt ctactgtttc
    15361 ttctactact tcttctttag cctctcctgc tttgtatcct tctacgttat ctgtagtcat
    15421 tggagaaagt gcatctaaag tttcttttaa cttagctcct agtacttgac ctatagttaa
    15481 tccattttct ggtattgtta ataatccaga atcctctaag tctgcaaata ttgctggatc
    15541 ttctaagttt tctggcgcta taattgttcc cgcttcaaat ctacaacaac aaactgctgg
    15601 gtcatttgca tgggcttgag ctgtttctaa agttattgac atagtataaa atacctcctt
    15661 acattatttt cttatttatt attttaaatc tcaattcctc aacaggtaac cccttaactg
    15721 agaaattaag taaattaaaa cctacataat ttgttatgat taatattatt tttttctcac
    15781 ttaaagtcac tttatattat aagcaattcc tatgccaact tattaacaaa aagatttttt
    15841 ctataataga ttcttcatct atcgctattt ttagccaaat attatataaa tatttattta
    15901 aaatatgtta ttttttgtat ttgtaaacat tttctcaaac aaaaaaagtg tcaattaatc
    15961 aacacttttt catttttttt aacacatcta tatacacaac acttatataa gcgttggctt
    16021 tactaggttt taaattaaag tttatcattt tgtcaaaatt tagtcatgtg tcatatttta
    16081 gacacatcta ttttgtattg gtctatctta taatacaaag ttgttctggg tatatttaat
    16141 atcttagctg ctttagcttt atttccatta gacattttaa gtgccttggt tatattcttt
    16201 atttcaattt tttgagttgc cttcgttaaa tctaaaggat actcttcttc ttcagtacta
    16261 ttatttgtag aatccatcat ataacttggt acatcatcca catctatttt agaattttgt
    16321 gacaatacaa caatattttc aatcgtattt tttaattctc taatattacc tttccatcta
    16381 taattctgaa atatatctat tacatctttg ctaattgtaa gaacaggctt attattttgt
    16441 ttacatatct cctctaaaaa gctatgaacc aataatccta tgtcttcttt tctctctcta
    16501 agtggaggaa tctttatttc aacaacattt aatctatagt ataaatcttc tctaaatttt
    16561 tctgccttta ccattttact caaatcttta ttggtagctg atattattct aggatttatt
    16621 tttattgtgg tatctccacc aactcttctt atttcttttt cctgtaatac tctaagcaac
    16681 tttgcttgca tactaagagg taaatcggca atttcatcta aaaatacagt tccatctttt
    16741 gcaagttcaa atatacctat tcgacccttt ttacttgctc ctgtaaacgc acctgattca
    16801 tacccaaaaa actcactttc aaataactca tttggtatgg cactacagtt aacaggtata
    16861 aattgacctt ttctctcact ataatcatga atagctcttg caaatacttc tttacctgta
    16921 ccactttcgc cccaaataaa tatgctacta tttgttctag ccacttgttt tgcaatcgct
    16981 ttcgattttt ccaatttata acttttacct aaaatcttat caaaactacc tttagataaa
    17041 tttttgactt cgttctttag atattttaat gtatcattta ctttttcaag ttcaagagct
    17101 aatatcttag cttctgtaac atcaacttct gtacatacaa caccgataaa ttcttcttta
    17161 taaaatatag gatttgcatg aactaatgca tacatatttt cttttttatc tgtaaaatac
    17221 aagtcactca ttccaacttt actatttagt actttttctg aaatagtatt ttccaaaaat
    17281 tcattcatcg gtcgaccaac tatatcttca gactttatat catacctttc ctccataaac
    17341 ttattccaca agataacaac accttcgtta tttatagcac aaattccttc tttaatttga
    17401 cctataatat acttcaaagt taatccataa tcttctaaat gcatatatag atagtcccta
    17461 atgtgctctt gttttagaac tccaataatt ttattatctc taagaacagg aagaatccct
    17521 atatttttta atatcattat atctctacat tcatctaagg ttaaaccttc gttgacatat
    17581 attacatcct tcttcattat aagttttact ggttgacctt catacttttt atgcatctca
    17641 taaagattat gtatatccgt catggatata atgcctttta attggtcaga tgaatctatt
    17701 accattagag tttttgtgtt gctttttatc atttctttta ttgcatcttc aattcttgtg
    17761 tcttcgtcta tagttgtaaa ttttgtatcc attacctctt ctactttttt tacttctggt
    17821 atcgaaaaca taaagtcccc cctttatagt agtacctcat caaaacttat ttatatatat
    17881 ttattctagc atattataat aattatttat taaataatta ttattaaaat ttattaagta
    17941 attagcattt aaatttatat aatcacttta aaatttataa ttaaatttat acaaatgaaa
    18001 tcaaatgaat tataccctta gtatattatt ttttcaatac tattttcacc ttcaaaacat
    18061 ttttctattc atcaaacaat taatgaatgc ctgtatccaa tcataaattt aagaatataa
    18121 ttaaaatata tatttttaaa caaactatat ttgcatatta tatttacata aaaaagcccc
    18181 aaaatgactt ataaatcaat ttgagacttt ttattttaaa cttaataata atctagatta
    18241 atttcttttt attttacatt gatacgctaa tccacaaatt gcaatgacac taataaatac
    18301 atatgacatt agctgagttt catcacctgt ttttggtgat tttattattg tatcatctct
    18361 tgatagttct ggagtttttt ccactgtatc acttactgat ggtactggtg gatttaacac
    18421 tgtatcctct gttggtggca ctggtggatt tagcactgca tcctccgttg gtggcactgg
    18481 tggatttaac actgcatcct ccgttggtgg tactggtgga tttattattg tatcatccat
    18541 tggtggtact ggtggattta ttactgtatc atctattggt ggtgatggtt ttggcttatg
    18601 tccatttgta tcatctgttg gtggcactgg tggatttaca acatcatctg ttggtagacc
    18661 tatattaaag ttaacttctt gaccattttt tttctctatt gtataactta ataaattttg
    18721 cttactagat ggtaatgaag atgaatggtc tttttgtcct aaatcaagca ctgccttata
    18781 atattgaata gcttttggaa aaatataatc ataagataca gcagaaatat tagctttggc
    18841 atctggtttt acaacactct taagtttaaa tttctgttgt gttgaaattt ctacttcacc
    18901 tgtacctatt atattaccat ctccatcata agcagttact ccatctggta gtcttaactt
    18961 tactacacca ctataaccta ctaaggctac agcttctgtt tcataagtac catcttcatt
    19021 tttttcaaag ttaatcttag ttaaaccatt actcaaacta ggttttcctg tatcatttgg
    19081 ctttaactct tcacgacctg cttttaacaa tgcttctaaa taagctttta tatttatagc
    19141 tacttctttc ttcttctgat actcagctct ttcctcttca gtttttgctc tttctataag
    19201 tttatcacaa aggtttatag aaccttgata ctgcttaagt ctttcttgtt catccatttg
    19261 tccaagaata atccaaattg cactttgagt agctgagtaa gtcatcaatt cattttcttc
    19321 tccacctaaa ttaaattgtt gcttatatcc aaatccatca aattcagaac ctgcatatag
    19381 tatagattta actgcttctt cattttggtt ttcttgacca ttaaaatctt ctgctggtaa
    19441 atatggactc tttccattac tacttggtgg tcttaaatga ttattgtaac aaaatgcagt
    19501 ttgctctcca tcttcacctt ctattacata tttgtcgtct tttgagcttg ctcctacaag
    19561 accctttatt acatagtcaa aagttaaagt ctttgtaata tcatcaaatc ttaaatcatg
    19621 ataaggtcta tctgaaccat ctatttcttt tagtaaacaa gaatttttta cttttgcttt
    19681 tttgtcatac cataagctaa taaatgagtc ttctgaagta aaaacttggt taatttctga
    19741 ttgttcatct gcctttacat cttcttcatc ttcccaaaag ttatatacta ttttatttat
    19801 ttgagataaa gtcatatcaa gaattttatc aatatcttgt tttaaactca tttcctctat
    19861 aactatttct tcttccaatg gtttttcttc ttctggagtt tgctcatcta atggtttctc
    19921 cccatctggc atctgaccat ctgatggttt ctctacatct ggcgtctgac tatctgatgt
    19981 cttctctcca tctggcatct gaccatctga tggtttctcc ccatctggta tctgaccatc
    20041 tgatggtttc tctccatctg gtatctgacc atctgatggt ttctctccat ctggtatctg
    20101 accatctgat ggtttctccc catctggtat ctgaccatct gatggtttct ccccatctgg
    20161 tacctgaccg tctgatggtt tctccccatc tggtacttga ctgtctgatg gtttctcccc
    20221 atctggtacc tgaccgtctg atggtttctc tacatctggt gtctgaccat ttgattgttc
    20281 ttctccatta agtattgtat cattgctagt cacttcgtca gcataagata ctattggtgt
    20341 aaatgcagta aacaccatac taaaagcaat tactaaactg attatacttg attttttaat
    20401 ttttgttttc aaaattctcc tgatactaaa aacatatatt gtattcactt aatataatta
    20461 aaattattaa atattatatg aatcttattc aataatacat ctttagtgtg ttcaattagg
    20521 gtcgttactc ctaaccagtt ctcctatgaa cttcttaata tttctattaa gcacagacta
    20581 tatcattacc taaccataaa ttaggtactc cccatttccc cacacttgta ggtacgagat
    20641 ttctctctag tcgttgaagt ttcttctttt cgaagcttac ctgctgatta tccattttca
    20701 aggcacttag gatttaacct tatgctatct aactaatttt ttctactttc gtaacctcaa
    20761 gtgcttatta aatgcacact ctatcacact tatccttatt tcatgattat gttgtggttt
    20821 agttagcttt aggaacttcc agcaattaaa ggagttttgg gcagatatct gccactctac
    20881 actcgttatg aatgcaggga gcatttcaaa aatatattta tatctatctt ctattaatta
    20941 cttatttatg tatttatttt atatattttt gacaattaaa agttactcct atttatattt
    21001 atagcaggtt gcactactaa ctccatatat cttttaagtg cccaggttta agatatactt
    21061 cacagtttct ataaataggt tttttaattt ttctttttta actttttaag tgcacttttt
    21121 taacaactca ataaaatata aattagtatc ctagatactt atctaataaa agacaaaaat
    21181 tagtttttat aataaaaaag atactataga gtatcttcaa atatcacaaa aaccttttac
    21241 tctatcggat agttgactta atataatatg catattaata caaaccacat acttgagtat
    21301 cattattttt gtccaatatt aattgtaata gctattatta tcattataca atatctagca
    21361 tattttgtct attttttcca tttattttac aatatatata ttgccctcaa aatacattaa
    21421 aaaacatcct tagaagtaac ttttaagttt tacttctaaa gatgtctaac ttctatttat
    21481 ctattatttt atttttaatc ttacgtcacc ttttattctt cttattaatg ggtgattaac
    21541 tgttctcatt acatggtcag taatatgata tacacctaat tttaattttg gtgcagaacc
    21601 cattactgtg ttactacagt cagtacagtt tcctattaat ataacttctg ctccagcttt
    21661 cttaaattca atacattttt gagcttgtcc tggacagtgt cctggatttt cacattttaa
    21721 tgcaccttta atttcttgag cttgtttaca tttttgaact gatactatat cagtaactcc
    21781 cattttagtt gctatatctc tcattctttc ttcattaggt ccacatgcac aaactaaaag
    21841 acccattttt ctcttttcat taacaaatgg catagtaact attataccac cactagtttt
    21901 agttattgga gcatctaaag tagtagcttt accagtgaaa ggtcctccta ggattatttc
    21961 accatattca ccttttattc caccagctct ttcaattaat tctccgacag ttgttccaat
    22021 tggcacatct tcaaacacta tagattcttt tccactgttt aattgaccaa ctacagttat
    22081 atttttagat ataactggtc ttttttgctc aacagcctct actattctag ttagtgtttc
    22141 tacgtttatt actactgcat tagcctctga aggtaattga gttggctcaa gtaattttcc
    22201 tagaacgtct cttactattg ctctctcttc tcccattggg tacatatcaa ctagttctgc
    22261 aacttctatg ttgtctcctg gctttattac ttttttgaaa gcttctattg cttttggatg
    22321 cttacttttt attgctagca taccttttcc agcattagta acttccatag catattttat
    22381 accttgataa actatttctg gtctttcttc tatttctttt atattatgag ctaataaagg
    22441 ttcacattca gctgcatttg ctaatattgt tccaccattt aaatctatgt ttaattttac
    22501 atgagtaggg aatcctgctc cacccattcc tactactcca gcttctttaa ttaagtcaag
    22561 tataccttct ccttttaatg gttcaaaagt atcttcttgt acttcatcag cttcaattac
    22621 tatcgcttgg tcatttattt cacttatttt tccactaaca ctggcataaa tatttgcccc
    22681 taatccagtt ggttctgcga ttagagttcc tttttttact acatctccaa cacttactac
    22741 tggcttatct ggaccaccaa cgtgttgttt taataaaata gaatatagtt tgttcaacta
    22801 gtacgccccc ttaagtatat taattttttt ataagctgtt caaataatgt taatgctatt
    22861 tttctattat aaggtcttaa catattgttt atttctatag aacatatcgc ttattaacaa
    22921 agcaatttct atgccaactt ttaaacaaac ttataccctt tggaaatact gtcttacagc
    22981 attcattaat tcttctaaat ttttaaaccc atcaaaaaaa agtcaattat tagacacttt
    23041 tttaatcaat ttaatctata attattgata ttgcatacat gtataaaaag tgtcaaaaaa
    23101 tagacaccgt ctttttatcg acacttttct aacatatact atataattta tcacaacttt
    23161 acttaaaata tacatttatt acttattaat tcttagtttt ttgcactcca ttggaatcaa
    23221 ctacataatt atctttatac aacaaatcag atattttgac tagactgtat ccatcttttt
    23281 ttagtcgact tattatagta tccaaatagt tatctacatc atttatatta gcatggaata
    23341 aaactattga accaggttga gattctttta ttactttttc aattacatga tttggtccaa
    23401 gctccttcca gtctatagaa tctacatccc acttaattac tttataacct aaatcactac
    23461 aaatgtccat agctttattg tttacgtccc caaaaggagg tctaaataaa ttagtccttt
    23521 ttcctgttat attatatatt ttttctgatg tagtttctat ctcttttgtt atgtcttctt
    23581 ttgatatttc cttcaaattg gcatgagtat ttgaatgatt tccaatttca tgtccttttt
    23641 catctatagc ttttacaatt tcttcattat catccaccca actaccaact aaaaagaagg
    23701 tagcttttac attatgttta tctaaaattt ttaaaatatc atccatatta tcagttcccc
    23761 atgctacatc aaaactcaaa gctatttttt tctcatttgt atcaacttta tatataggca
    23821 tttttgaatt gtcagattta gaacatatgt attttattcc ataaacaaaa acaaatatta
    23881 acactaataa taatgaaaaa aataaaacac tatatatcaa agctttagtc cttttaactt
    23941 ctcttttcat actattcccc ctttgaaata atttcttcta tatttaaaat ttattttttt
    24001 tcagctcaaa aaagtactgt aatatttaat ttataagaca ataactaaaa aaataaaacc
    24061 tatattgacc taattaaact ctctaaaata ctcatttatt taattttttt atatttttta
    24121 catttttacc tttatttttc tgtatatatt atttttattt tggagaaatt agtattaaca
    24181 tcacataaat aaaagtgttg ttagacatca taaactaatt atttgaggag gaaatttact
    24241 atgtcaaatt caaacagaac agtagttcca gaagcaaaag ctgctttaaa ccaaatgaaa
    24301 ttagaaatag ctaacgaaat aggtttatct aactatgaaa acatagacaa aggtaactta
    24361 acagctagag aaaatggata tgttggtgga tatatgacta agaaattagt tgaaatggct
    24421 gaaagacaaa tggctggaaa ataaatagta aattttgaca cctgggtatc aacagggctg
    24481 ctaattagca gccctgtttt tctatattta tttataaaat tgtttttata tgaattatta
    24541 aagattaata atacaataca aattgttctt ttaattttgt acaaattagt ttaatatatt
    24601 atatatagaa atagtttgac ggaggatgat atttatgttt gaaaactcgt ctgaagaatt
    24661 agcctatcac aaacttttaa ttttatatat attagaaaaa attaaaatgg atttaactaa
    24721 ttctcaaata actcaagtag tattagagac tgagatgatg aattattttt cattacaaca
    24781 gcttttatct cagcttatgg aatctaagtt tttgactaca tataaagact ctgacagaga
    24841 atattatgct cttactcaaa gaggcgtaga aagtctagaa tacttcttta atcgaatacc
    24901 tactagtgtt actgaaaaaa tagacaagta tatagattct aacaaagaaa atctactagc
    24961 agatactcaa gtaaagtcta gttttgtaaa acaaagtgat aatgaattta ttgtcaacct
    25021 tagagtcata gaaaatcagt ctaacttaat agatttaaat ctaaatgttt catcagagaa
    25081 acaagcaaaa cttatttgca ataactggaa aaataatgca tcttatatgt atgccgaagt
    25141 tatcgattta cttataagag acatacatta aagcctacaa acaaattata gttgtaggct
    25201 cttttcctac cttgatttct tttattaatt tgttatttgc agtttcataa acttttactg
    25261 aattttcttg tgtatctgat acaaatagta acttttcatc tttagatatt tctaatcctt
    25321 ggggaatccc atttatttca atatttccaa tgtatctact cgttgtataa tctaaaattc
    25381 ttacattatt ataacccaaa tcagcaacat aaactttttt gccactataa ttaaatctta
    25441 tttctactgg taataaaaat tcatataata ccttttttat atccatgctt acttcatcta
    25501 ttattataag ctttccatca agtgttgata tatacatttc tttcttttgt ttatcaagtt
    25561 ctatatgcca agctctaaag tccatattta ttttcttttg tatattttta ttaattgtat
    25621 ctatacatac tatcgagttt atacacggta catataactt tttgctttct ctatcaaagt
    25681 caaatccatg tggcatatta tcaactccaa taattcctat tggatttaat gtgtttttat
    25741 ctagtacata tatactatta gaatcttcat ttgccacaaa caattcatta tcgctaagta
    25801 caacttgact aagacttcct cctatagaga cagtatcaat tattttatca ttacttaaat
    25861 ctaagacata gagtatccca ttacttgaac taggtatgta tattaaattt ttttcttttt
    25921 ctatgcaaaa atggtgtgga tatatattat cttctaatac tatttctttt tctagttcaa
    25981 gtgtagaata atcaactatg ctaatactct ttgataagta gttagaaata tagattttca
    26041 tataacccct cctctctaca atacatatac tacaataata tattatttca ttagcttgta
    26101 ttatgcttta tagttgcatt attacgcata tatttggtat tattattaat gttaaaaaaa
    26161 gaaataatat ttaatggagg tctttttaat gaaagtaaca tttaatccaa gtggtgtatg
    26221 ttgtagagaa atgatttttg aagttgatga aaataatgtg attgttgatg ctgagtttat
    26281 aggaggatgt aatggtaatt tatcaggact tagaagctta ataattggcc aaaatgctct
    26341 tgaagttgct gataaattaa atggtattga ctgtggtgga aaaggtactt cttgcccaga
    26401 ccaattatct aaagctatac gtcaatctat ataattactt atataaacat cagtaaaatt
    26461 attaaaaact tattttatat aaaaagagtt gtccattttt tagacaactc ttttttatat
    26521 ttttatttgt atacttattt tataaaatta taatttcttt actaaagttc tcactgtacc
    26581 aatcatatat aatgaacctg cacttattat aatatcatcc tcacttgatg tttctaatgt
    26641 ataatttact gcatcctcta tctcatgctt tgatgttaca tcatctacat attttaatac
    26701 tttttctctt aatatatctg aatttatggc ccttgggtta tttggtgttg ttgttattac
    26761 cttattaaaa tgtgacaaca atatctctaa aacactatca atatctttat cttctaacat
    26821 acctattaat aaggttaatt ttctaccttt aaaattctta tcaagtgctt ttgccaaaga
    26881 ttttgctcca tcttcattat gtgcaccatc tattataaaa attggatttt cctttatctt
    26941 ttctattctt cctggccatt tagtatttat aagtcctttt cttatagatt cttcatttat
    27001 atttgctaat ttatttatgt cttttagata ttttattaca ctaattgcca agatagaatt
    27061 gtttatttga tgttcgccta tcaattttat ttcaaggtct ctataagttt ctttcattac
    27121 agtacaatcg tatatttgag aatttatatc tgattttttt atgtttatat catcaaaatc
    27181 aacttctata tatttagctt ttttctcttt acaaactgac tttataacat cctttgcttc
    27241 atcagtctgg tcatatacta gtactactcc attttccttt attattccag ccttttcgta
    27301 cgctattttt tctattgtat ctccaagtat acctgtatgg tccaaactta tagatactat
    27361 cacacttaca agtgattttg ttattatatt tgtagcatca tatctgccac ctaaaccaac
    27421 ttctaatgcc acaaagtcta ccttttgttc tgaatagtaa taaaatgcca ttgcagtaac
    27481 cacttcaaat tctgtcggat atgcatagcc ttcttttacc ataatctcta ttttttcttt
    27541 tattaactca attattctag caacatcatc ctgtggtatg ttttgtccat ttactcttat
    27601 acgctcagta aaagtttcta aatatggaga tgtatataaa ccaactttat atccacactc
    27661 tcttagtata ttagaaacaa aagaacacac agaacctttt ccattagttc cagctacatg
    27721 tattatattc aaagtttctt gtggatttcc taataattct aataactttc cgatattttc
    27781 taatcctaat cttataccga atttattagt ttgtgatatg tactctaatg cttcttcata
    27841 tttcattatc taactccccc tgtattggca ttttctctta tgctatctaa ttatagtata
    27901 ttttttactt ttaatctacc gacaaagaat acatataatt taataccttc tacattaaat
    27961 ttagctacaa atctttattt ataagttttt atatctttcc actaattcag tcagtgtata
    28021 tattgaaact aagtcatatt ttgagatgtc catataatta aactttatgt caaatctatg
    28081 ctctagcttt ttcaacagat taaaacaatt atcataaacc aagcctatct ccttattaaa
    28141 aaggctaccc ttaaagagtt tttcattatt ctcttcaata tctaagcaat ccctaacaac
    28201 gtcagcaact ttcataaaaa gagcattttc tttgtcatac aaatccaatt taaagtttac
    28261 aattctacta cactcatcaa aatcacaact atcccaatga tttctagttg ctaatgattc
    28321 aagcttatca tttaaatcag atattgttat gcctggtttt tctgataata tcttagctat
    28381 tttaccagta agctttgctg ataaataact attacatttt ggtggtaatc tataggaatt
    28441 atttatatca caacttatat atgaattgct atccataaca cattgaacat catatttttt
    28501 gttgtaccaa aatgaatcat ctccatctaa agtaaatcct cttacaccta taacattgtt
    28561 aaacatagct ggataactta agtcaaaatc attagcgagt gaacatacta ttatttttcc
    28621 ttttctaaac aattcatagc aaacttcaaa taagccctta acactttcta ctcctatgac
    28681 tgataaactt aaattaatta atcttatgtt agtgtctaaa aggtatctta atgcttcttc
    28741 aagaactttt atatttgtaa taccatcttt tctcaaaatt tttattacaa ataactctac
    28801 atcttcatac tctttcttta ttattgatgc acaagcagtt ccatgaccat cttcatcatc
    28861 aaatttatct gatataaaga tataatcatg catacattca aaagcaattc ctccaactaa
    28921 attatctttt aagtaatcat gttctttgtc tattcctgta tctaatactg caaccttaac
    28981 tttattattc atatcacata tattctccat tacaaaagtt tttaatatat ctatgttaat
    29041 agttcatctt aaactttgat ttaagttgca caatttatct tttaaatgct atatttcttg
    29101 ctaaaaacat aaaataagta caccatatat ttaatttata tttattatat caattattat
    29161 cattaaatgg ttaaatgtta agataaaatt tttagattta tatcacaatt tagaattatt
    29221 tgtaaaaaag gagattatct ttttattaga caatcccctt gtatatttta ttaatattta
    29281 tctatgatta agcatcaatt ttattttata ctttttgatt ttaatactat acatctaaat
    29341 cttatttagg gggaacaatt atttatatga tataaaaatg ctaatttgaa tgaacttgtc
    29401 ttataatcag ttttagaaaa tacaccattc tacaaagtat gaatcaaaag gtgaaaaaat
    29461 actaaaaata ataaatcaac atcaatttta atcaatatat gaaaaactta taccaagtat
    29521 gtataaaaac taatataaat attattttaa caactaatac ttacaccttt atatattata
    29581 tcatatttca ataaataaat tgcataaaag gaaatctttt catagaatta aactatttat
    29641 taattcatta
//
LOCUS       DAELTA010000033        29409 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.033, whole genome shotgun sequence.
ACCESSION   DAELTA010000033 DAELTA010000000
VERSION     DAELTA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.033"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(114..983)
                     /locus_tag="KPG13_001267"
     CDS             complement(114..983)
                     /locus_tag="KPG13_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985004.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1080..1937)
                     /locus_tag="KPG13_001268"
     CDS             complement(1080..1937)
                     /locus_tag="KPG13_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0985005.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2152..4170)
                     /locus_tag="KPG13_001269"
     CDS             complement(2152..4170)
                     /locus_tag="KPG13_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0985006.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4441..5217)
                     /locus_tag="KPG13_001270"
     CDS             complement(4441..5217)
                     /locus_tag="KPG13_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0985007.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5274..6245)
                     /gene="asrC"
                     /locus_tag="KPG13_001271"
     CDS             complement(5274..6245)
                     /gene="asrC"
                     /locus_tag="KPG13_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG0985008.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6258..7049)
                     /gene="asrB"
                     /locus_tag="KPG13_001272"
     CDS             complement(6258..7049)
                     /gene="asrB"
                     /locus_tag="KPG13_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG0985009.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7050..8057)
                     /gene="asrA"
                     /locus_tag="KPG13_001273"
     CDS             complement(7050..8057)
                     /gene="asrA"
                     /locus_tag="KPG13_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG0985010.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(8448..9137)
                     /locus_tag="KPG13_001274"
     CDS             complement(8448..9137)
                     /locus_tag="KPG13_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0985011.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9200..10048)
                     /locus_tag="KPG13_001275"
     CDS             complement(9200..10048)
                     /locus_tag="KPG13_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0985012.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(10067..10954)
                     /locus_tag="KPG13_001276"
     CDS             complement(10067..10954)
                     /locus_tag="KPG13_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0985013.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(11012..11815)
                     /locus_tag="KPG13_001277"
     CDS             complement(11012..11815)
                     /locus_tag="KPG13_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985014.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(11975..12427)
                     /locus_tag="KPG13_001278"
     CDS             complement(11975..12427)
                     /locus_tag="KPG13_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG0985015.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(12557..14059)
                     /locus_tag="KPG13_001279"
     CDS             complement(12557..14059)
                     /locus_tag="KPG13_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0985016.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(14092..14958)
                     /locus_tag="KPG13_001280"
     CDS             complement(14092..14958)
                     /locus_tag="KPG13_001280"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG0985017.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            complement(15043..15714)
                     /locus_tag="KPG13_001281"
     CDS             complement(15043..15714)
                     /locus_tag="KPG13_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG0985018.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(15987..16478)
                     /locus_tag="KPG13_001282"
     CDS             complement(15987..16478)
                     /locus_tag="KPG13_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0985019.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(16500..17864)
                     /locus_tag="KPG13_001283"
     CDS             complement(16500..17864)
                     /locus_tag="KPG13_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0985020.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            18283..19458
                     /locus_tag="KPG13_001284"
     CDS             18283..19458
                     /locus_tag="KPG13_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0985021.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(19631..21028)
                     /gene="asnS"
                     /locus_tag="KPG13_001285"
     CDS             complement(19631..21028)
                     /gene="asnS"
                     /locus_tag="KPG13_001285"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG0985022.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(21105..21338)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21507..21701)
                     /locus_tag="KPG13_001286"
     CDS             complement(21507..21701)
                     /locus_tag="KPG13_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG0985023.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(21974..23647)
                     /locus_tag="KPG13_001287"
     CDS             complement(21974..23647)
                     /locus_tag="KPG13_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0985024.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(23657..27055)
                     /locus_tag="KPG13_001288"
     CDS             complement(23657..27055)
                     /locus_tag="KPG13_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0985025.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(27222..28418)
                     /locus_tag="KPG13_001289"
     CDS             complement(27222..28418)
                     /locus_tag="KPG13_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0985026.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(28439..29260)
                     /locus_tag="KPG13_001290"
     CDS             complement(28439..29260)
                     /locus_tag="KPG13_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG0985027.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         9491 a         5275 c         2984 g        11659 t
ORIGIN      
        1 ttttaataaa aaatcatgct acatcaatag taagcaacta ttaatgtagc atgatttttt
       61 gtatctaaat aatcttagta tttacctatc agtatagatt gatatatcaa aaactatagt
      121 cttctatatt cttctctttt tagcttttgg ttacttagat agtaatcaat ttcatcaatt
      181 agacgttgtt tatcatcagg taatacacct tttaaattag cataatttga tgcatgatta
      241 cttctaaata caattttttc attagcatct atattcttta tcatctcttt aatttcttgt
      301 aacacagcct tatcctctag tattgtaaac tctcctgcct taagtaaatc gtaaagttca
      361 gtattaggtt ctaccattaa actaagtaca cccacataat ctggtgaaat tgctcctagc
      421 atttttcctg tatcaactgc atgctgatgt gtttttttag ttcctccaag acctgctatt
      481 acagtggctg ataatttaat cccacttttt ttaacttttc taccaacttt tattaattgt
      541 tcactactaa aacctttctt aatatcttct aatacttcat tgcttccact ttcaagccct
      601 agataaatca ttttaacacc aagttttctt aattctttaa gttcatcatc agattttttc
      661 tctatagatt taggagagcc gtacatagta attctttcac actctggaaa aacttctttt
      721 atatataaaa taatatctct taatttctcc attggtatta tcaatgcatc tccatctgct
      781 aagaatattc tttctacatg cttataatat cttctaaata tatcaatttc attttttatt
      841 acttctagtg gctttatttt aaatttttta gatttgtaca tactacaaaa cgtacatttg
      901 ttatgagaac atcctagtgt gacttgtatt ataagactat aagcctcact tggtggtctg
      961 taaagtggca tatcgtattc catattaaaa tcccccttgt aataaaatat tcattctatt
     1021 atattattaa tatttacttt aaataataac ttataaaatt atatttttta aattttagtt
     1081 taagatatat tctttaaaaa tttaatgata ctattggcag taatattaag ttgtttttct
     1141 tgacttataa tagcatcatt atctcccttt tgttttccat aatctccaaa ttgtgcatga
     1201 tttcctcctt caatctcaac ataagttgta tcatcaggaa gtttttgttt tgattctaca
     1261 agacttttaa aattgactac tccatctttg ctcccccata tagatataac tttttttcct
     1321 aattctttca actcatcacc cattggataa gaagctaaaa atacaacgcc ttcaattaat
     1381 ttattatcac gtgcaaaatt tgatgctgca actcctccta atgaatgacc tcctattacc
     1441 caatgttcta tgttgttgta agaatccata actttctgtg ccttattagt tccaagtata
     1501 gctagattaa gtggcatctt tacgattaca acttcatatc cactttctgc tatttttctt
     1561 gcaagtggag cataagattc tatttctact ttagctcctg gatacaaaat taaaccttta
     1621 gaaacttttc ttccttttgg tgtaaatgaa atgttttcat ttacagttac ttcaacttta
     1681 ctattgctga ctagagcttc ttttgccagc ttttgaggtt catatgtttt atttatccat
     1741 atgaaaaagc caagcaaaat caatacaaat agtataccta ttcccttaaa tatcttattc
     1801 tttatgttaa attttctatt attattttta tcatctttgt tctcaacctc atcataaagt
     1861 aaaggtctct caagttccat agttatacta aatgtatcat atttcttatt acgctttacc
     1921 tttacatctt tactcataaa tctaaccccc ataatatttg aatactatta tagtttatcc
     1981 taagtaaatt gttttttcaa ccaatgaaaa aaatcaatat tttaactatt ttcatataaa
     2041 attcataaat atatgttttt tgttttttat atattatttt cttttagtat tttataaaac
     2101 aaatagccat atactttatt ttaaatatat ggctattcta taatttaata tctaagctgc
     2161 ttcttttatt gaaactttag ttatattact tattctatac atttctttga caccatttag
     2221 agtttgcagt aagtttagac atattatctt gtcacctaga cttttagtat ttataatact
     2281 ctctaaaatt acacttcttc cttcatcata cttatctaca ttgtaatttg aaatataatt
     2341 agtatctctc aatatttttt caacatcttc atcttttatt ttatttttat taacatttat
     2401 atatagatta tacatattac ttattagtaa tttctgattt tctaaaaaat cctcttgatg
     2461 tttatcttta tatatagcat ttatcaacat aagtctatct tcaataagtg atggtatcaa
     2521 taataaattt tttatatagt aaacatcagc tacattttct atagacttat tgacttcatc
     2581 aattatattt ttaactattc ctttatacat ttctataaca tgttgatagc ctgttttagc
     2641 atcatatggt aatataaact tatttactat taatgcaata attgctccta atgctacata
     2701 tgaaatcctg cttatggtta aaacatttgg gtctcccata actactgctg aaccaagagc
     2761 tgaaacagtt acacatacca ttaattttct ataatcaact acataagagt ttatataccc
     2821 tgctaaaaga actattaacg accttaatgt actatctttt attattgaaa ataatacaat
     2881 aaatattaca gctcctataa atgtaccttc tattcttttt ctagaccttt gtatacaatt
     2941 ttctgcgtaa ggttgtgtta atgaaaagac agttaacatt atccatcttc catctcttaa
     3001 atggaaataa tccataataa aacccgctac agctgtagct aaagcaatct ttactgcata
     3061 agaaaatctt atagaattta aattaaagtt tttcttatat attgatagtc gtttaaattc
     3121 atgaggtatc tttgattttc tttcagcttt cttttcgtct cttgatttta cttttttata
     3181 atctactaaa aactcatata aattttctaa aacactataa atttctttta aatttatatt
     3241 tttagtatcc tgagcaataa cataatctct aaatcctaat atctctttat cagttatggt
     3301 atctttattt tctaaacaca tcttaatata gtttatttct ttcgttgcta caactagaac
     3361 atctgattta ctatttatgc tactttcttc taaaatattt tttatatctt catcttcaaa
     3421 ttctttacta tcctttttgt atctttctag gattaaactt attctttcta atgaaaataa
     3481 aatattcatt atgtctatac ctctaatact gatataaaag tcatcttttc tattatcaaa
     3541 aataaccatt ttcaaatttt tagatataga atttattcta taattacagc tatctatttt
     3601 tctcatacta gatttaatta tagaactatc atcattaata gtatttttta aaagactaat
     3661 tttttctaat aattcatcac atatagatat cagttcttta tctccgacat ttttcatcct
     3721 atttctattc ataacaaatt gagcaatcat tattataact gctccaacag ctaaagccga
     3781 caatctcata ggaaattcac tttttcctac aggtatactt accataaata tatacattag
     3841 accaaagggt acatatactg ctctttttaa gtcataacta aatacatacc ctattataaa
     3901 taatgctata aaatttaata aaactcctaa atacatattt tgtactgcaa aaaatgatag
     3961 tattcctaag attatattta ttgctaaata cttcagtaaa tttttaattg gagaaatagt
     4021 taaatctctt tccaagagcg ttagcatagc tgttactata cctacaccta taagtgtatt
     4081 tttacttcca aataaatata taaatataga aataaatgct attataaaaa taaacaactt
     4141 tgtttttgat attactagtt tctttgtcat catatccccc ctaaaaattt caacaagacc
     4201 aattttatca tatttgttat cttattcaaa atgcgctttt ttacggtagg actttaactt
     4261 tttttatatt tagaactata taattcatat ataaacgtat tttttatata tataaccgat
     4321 atattttcgt atgctcccat attttttttg tttcaaatat tttttcaaaa atataaaaat
     4381 aactctctca acaatactaa aaatgcattt atcaagagag ttataattta tacaataaat
     4441 ttaattaagc ttttctttac ctaatatata agttccaaga cccataaata aagctcctcc
     4501 aacaatattt ccaagtgtta cagctactaa attataaata gctcctccaa tagtaactgt
     4561 atttattgtt ggtgaaaata atgaaacact ataaattgtc atatttgcta cactatgctc
     4621 aaagcctgat gttataaaag caaataaaca taagaatatc ataattattt ttgcagtatc
     4681 actattagtt ctaaacgaac acaacactga tacacatact agaatattac ataaaatccc
     4741 tctaaagaaa agagctgtaa atggcatgga tgctttacta gctgctgtat ttgcaaaaaa
     4801 ttcagctaca ggtcctttgt ctattagacc agttcctaca aatataatgc ctaaaaccaa
     4861 agctccgatt aaattaccta cccaactgta tgcccatatt ttagaagtat cttttatact
     4921 tacaccttta tttaacatac cagcagacat aaccatattg tttccagtaa ataactctgt
     4981 tcccgtcatt ataaccaaac ttagtgctat tgcaaatgat aaccccatta ctatcttagt
     5041 cataggtgaa cctgcatcag tcaaaagtcc tccaatagta aatatcaaaa gtatacctat
     5101 acctacataa agtcctgcaa acgcagaaga taccaaatat tttactttac ttgtgtttaa
     5161 taaatttatc ttatttatag ctgcattagt taatttatcc aaagtttctt tatgcataat
     5221 tccctcttcc ttctattaaa ttttaattta ttcaaatata tgttttactc aatttataga
     5281 tatttaattc cactccagta tatgttttcg tacattttag ttatttctgg aagctctaca
     5341 ccttcaagtg cccatttttt aaattccata aatcctgttc tatcaattat atatcctata
     5401 tgttcttttc ctcctggtgc atctttatct atatactttt ctacatatct ataagtatta
     5461 agtattatct ttattatgct gtcctcatct acccataaaa gaaaatcttc tgctagtcta
     5521 gggtttttct ttcctgttct acccattata gctaatctat agtatttttt ctcatctcta
     5581 gtccatgcat ttgttgggca atttagcaca cactctccac agcctataca cttatcttta
     5641 tctcttacca ccttattatt ttctgttttt aatgctccaa cagatatctt agaacatttt
     5701 tttacacatg ctccacaatt tacacatcta tccttttcat atattggtaa agccatacca
     5761 ataattccaa agtcattcat tctagctttt atacaatcat ttggacaacc agttaaagcc
     5821 actttaaaat gtaaatcatt tggaaatata gctttttcta ttcttttagc aaattcagtt
     5881 gtattatatt gagcctttgg acagacttta tttcctatac aagctgctat atttcttgta
     5941 cctgctgctg gataacctgc gtttttctca ctttgattta tattcatttt ttcaataaca
     6001 ggttgtataa ttttatttat ttcttccata tcttccatat ttattcctaa tatttcaaac
     6061 ccctgtcttg tagttatgtg cacctgtcca ttgccgtact cacttgcaat tttagaaact
     6121 acagctaaac tctcagggtc aataagacct ccaggtactc taactcttaa agaagtctca
     6181 tattttgttt ttgtaattct ataagcattt ttcattactt ttttggtatt taaatctctt
     6241 atcacaaact tcacccccta atcaataaga tttttagcat atgaataatc aaatactggt
     6301 ccatctaaac atatgtaagt atcatccatt ttacaatgtc cacatttacc tattccacaa
     6361 cacatttttc tttcataaga aacccaaatg ttcttttcat ctaaatctct ttttaaaaat
     6421 tcttctactg taaatttcat catcattgga ggtccaacta caactataga agtattgttt
     6481 atatctttca tatccaattc tggtatgtat tttgttacta atcctacatt accactatat
     6541 ccttcctctg ctccatctac agtaactaaa atatcaagtt ttttactcca tctctttaaa
     6601 tcatctgcaa ataaaatatc ttttggtgat ttaaatccta ctattaattt aaacttttcg
     6661 catttttctt tattattata aaaatattca actattcctc ttacaggggc taatgcactt
     6721 cctcctgcca ctactacaat ttctcttcct tcataaaggg atacatcaaa tccatttcca
     6781 taaggacctc ttataaaaaa agaatctcct tctttataat taaacaactc gctagttact
     6841 tttcctacat ttcttattgt aaagtcaata taatcctctc catatcctga tactgatatt
     6901 ggactttcac cgtatttagg tatagatatc tcataaaact ttccaggcaa tacacctttt
     6961 gtattgcagt ttactctaaa agtccattct ttatcagtat gtttaactat atctagtatt
     7021 tttgcactta ctggtatata tgggttcatt tatacttcct ccttactaat tactaaatca
     7081 tttatttttt ctatacactt tgaaaatgat atgtattgcg gacaggcatc atcacatctt
     7141 ccacagccaa cacacatatg atatccgaat ctttttttaa aatcatgtat tttatgcata
     7201 gttttaaatc tcattctttc gccttgtttt tttctaaaag aatttccacc tgctatatct
     7261 gtatatccat ctacttgaca agaagcccaa acccttcttc tctcacctac attttcgttt
     7321 tccttataga aaatatcctg cattgtaaaa catgaacatg ttggacatac aaaattacat
     7381 ctaccacatc ctatacatct tgtatcatat tcatcccata ttgaatttcc tatcatttca
     7441 tttgaatcaa tattctctgg cacatctaca gatattaaat tatcagttac ataatcaaca
     7501 ttaaattcag ttgcttctcc attaaatact tcaaattcac tatcctttat atctaagtac
     7561 agtgtatctc cttctatatt taatccaatt gaataattat cagttttgtt agaatccatg
     7621 ctcacacaaa aacagtttct aaaactttcc ttacacccta caagtgcaaa ctttacttta
     7681 tctctgatat ttttataaaa ataatctttt tcttcaccat tatttagata tatttcatca
     7741 agtcttttta taccatttat gtcacaagct cttaagaaaa ttaaaatttt cttgttgtct
     7801 aaatcgcttt ccttaaactc tttttcagta aaataaaaca atacttgatt tatgggaagt
     7861 acaacttctt ttggtgaaaa gtttgctttc ttagcaaatt ccatttcttc aaaagcgttt
     7921 acctccttgt atcttataac atcagtatct gaaaaagtac ctttaaatgg gattaaaact
     7981 ggtgcaaata tcttgtagtc tttttttaga taattcagac cttcattgaa cttagatgaa
     8041 tctaatttta gtttcatttt tatcaccttt cttatatttt agttttactt acttgctatc
     8101 ttgtttaaat cttaacactt tattttctat gcttccgtga taattatcac agtttcaaaa
     8161 ataataaaaa aaatattaat ggagtttatt aagttctgtt aatatttttt tattatctac
     8221 attattcgat ttcaaatcta atcacttaaa tacaatcatt aagtacaata aaactactat
     8281 ctactcaatt ttttgaataa aaaagaatct tatttgtttt tttatacaat tcaatagggt
     8341 aaaataaaaa ttaataaatt agacaataaa aaagaatcta taaatagatt ctcttaataa
     8401 tatttttatt taattaaaat tctaataata ctcttactta atcataatta attattttta
     8461 aaataccttg ataattcagt ttctttaaca taaaaacctt tattgtcaat tttaataagt
     8521 tcttcttttt gcaatacctt tatcattctg gaaacagtct cccttttaca gccaagcata
     8581 tcagctaaat atgtaactgt aatatttaca tttagtaaag tccattctcc acagcaaact
     8641 ccaaattctc tacttaacct atataatttt gctgcaagtt ttttatctac ttttatggat
     8701 atagaatttt tcaactgtct atataaacgt cttattcttc tttccatata agaaataata
     8761 tcctttgtaa gttgaaaatc attttgcata atttctaaaa attcttcaat actataataa
     8821 gctacaattg aattttcaaa tgcttcacag ccaacagtag agtttttaga agcgtcaagt
     8881 gtaatatcat ttatgacttc cccactattt aatatgaaaa ttacttttct ctctccattt
     8941 tcacttatct taaaaataga tacctttcct tctaaaagta tataaacact gtctaagcta
     9001 tctttttcat aaaataaaat ttcttttttt tgtaatcttt ttagtttaac tttttcttta
     9061 agttgattct tcgttttagt acttaaatta ttaaaaatta ttactttatc aatattataa
     9121 tcttttaatt tagacatgta atatctctcc attcctaaat ttttaagaga gctctataaa
     9181 acattacaaa tttcttattt tattcatttt taaacaattt tatagcattt gtagttttaa
     9241 ttttcctttc aatagcttca ttagaaagtt cttttactac ctgtgtatat actaaatcaa
     9301 taatgaaaaa ttgtgctaat ttagaagata ttgaaccagt ttccagtact gtttctcccg
     9361 atacataaga taagtgtaca tctgaaatat cctttaattc agaattttta ttttcagtaa
     9421 ttgatataat tttagcatta ttggctcttg caagctttac agtttttata atctcttctg
     9481 tttcaccaga atgtgaaatt gcaattatta aatctccttc ttccattatg gaagacatca
     9541 ttatcattgt atgactactg tcaaaactta cacaattaag acctattctc ataaatttat
     9601 agtttgaatc ctgtgctatt ataccagaat atccaatacc tatgaagtaa actttctttg
     9661 catttattat aagccttgcg cagtcatgta catccttact attgattatc tctactgtgt
     9721 tctctaaagt ggttacattt gaacttaaaa gtttttttgc tgtatccaaa gctggctcat
     9781 cattttgtat atttttattt ataatatttt ttttattgat agttgatatt tcttgtgcta
     9841 aagtaacttt aaaatcctgt aatccattaa aattcatttt ttttacaaac ctagttatag
     9901 ttgcttcacc aatattgctc tctttagcta tttgtgatat tggtttataa aatacatcat
     9961 ctatattttc ttttatataa gctattaata atttatctga ttttgtgact ttaaaagttg
    10021 gtgattccaa ttgttctaaa atactcatta aaaacccttc tattctttat attttgttta
    10081 aaaaattata taatgctcca agcattcctg catcattttg tctctttgca aattctatat
    10141 tagtgttttt gtaaactctc tctataagaa cctcctttaa tgctttgtct atttttggtt
    10201 ttaggaactt ttcttgtgcc ataatcccac cacctaaaat taccacttct ggatttatta
    10261 cataacatat atttgcgatt ccaactgcta atgattttat catattatcg agttctttta
    10321 aacaatcttc atcattattc tttgctaaat caaaaattat ttttccattt aagtcatttt
    10381 cattcaaatt ttttattttt gctacttttt taattagact gctagtagat gccaattctt
    10441 gaaaagaact tccatttaca ttcatatatc ctacttcacc agcactatta ctaaacccat
    10501 ttatcagttt attatttatt ataatacatc caccaattcc agtacctatt gttaaacata
    10561 tagaagatgt tgcatcttta cctgctccaa gccacatttc accaagtcca gcacaattaa
    10621 catcattttc aacttcacat tttatattaa actctttttc tacttctttt tttaattgca
    10681 tgcccttata gtttggaatt aattcttcta atgcaaatag tattttacct tcaactgggt
    10741 caaccatacc tgcagttgat atacatatac cacttatttc attttcctct atatattttt
    10801 ttactatatc ttttattttg gctaatattc cttctccacc tttttccttt gcttcagtat
    10861 caagacttga tttatcaatt ataaatccat cttctctaat aaaaccatat tttattgaag
    10921 tacccccaac atctatacaa acgtaatttt tcataacata tctccataca aattattttt
    10981 ataaattaac aactctattt aaaattttat attacacaaa tcttttactt atcttttcca
    11041 attcacagat atatttcctt gcttctgata agtctatagt atcacttaat acactttttt
    11101 ctttggttat atgatactta taaatatttt tacataagtc ctctcctaac atattaagac
    11161 cttcttgagt gtaatgaaca tcatcaatag ttaatcctct gtcatgaaac tgcatagcaa
    11221 ttttgcttcc tacacataat atatcacatt cactacaagc cgactcctgt gctgctgcaa
    11281 ttcgtcttgt tctaataaaa tattcatctt ctccaagcca ataaccagtt aaacttacaa
    11341 acattttatc aatcatacat tcttctttaa gcctatacgc aatttttttt agactttctt
    11401 tatagtacaa tacatcacta ccatattttg catcactttc accttgaatc caaataacgt
    11461 atttattttc tatattataa tgtttactta aggtagtaca gcctgtatta aatttttcta
    11521 tagtgtcatt tagaaaccag ttattatcat gattccagtt ttttattctt gaaccatctt
    11581 ttgcattgtg aataaaacaa actttatttc cagtaagctt attccattca tttgaaaatg
    11641 caggtgctat agtaccatca ccttctgaag tctctagctc acatctcata tttataattt
    11701 catcagtact caaatattca aaacaatttt catttggaat gatgctctct tttggatttc
    11761 ccaatcctct agcattgctt tgtcctatta ataagaatac atctatattt tccataagtt
    11821 aactccttta caaaatttta agcttttaat tattttcaat tttctcataa aaaactaagg
    11881 ctgaaaatag taatctaact aaaatttacg aagtctcact aatcgctgaa agttaaatca
    11941 ctattttcag ccctagtggt tatataaatt atcattatct aaccaaaacc ttaaaaatag
    12001 cttttttgat attttctttt tctaaaactt ttattcctgt catatgagca tcttccggat
    12061 aacaaactaa aaaatcacct tttctaagtt ccatgtatcc attattttta ccatcaagag
    12121 gcaagaaatc gcccttttct tcatatattt tttgttctaa attttctata aaatttatat
    12181 ctattcgttc acatccatca agcatcaaat gaacatctat atacttctta tgagcttccc
    12241 agaatctatc ttctttttca caagttttat actctacaat attcacaaaa atatcatcac
    12301 catctattgt ataagtacct ttttcataat ctattaatga attatcttta gcatattcaa
    12361 aacatctaag taaatcttca tgcaatgttg aatatgtctt actatgattt atatttccaa
    12421 atatcataag cactatctcc ctatataatt ataaagtcta tttttatact tttatataca
    12481 aagtatatta tacaatctta tagagtaact aaattattta tctaatcact ctataagaca
    12541 atttatcatt taaatattat ttttcataat aaacagtagt ctcagctttt ggtaaagata
    12601 cttttgcttt aactttagct tctattgcac taactattgt acctaccact acagaagtcg
    12661 tgattgttat tagagaatat gcccaagaag ttacttctgg catacggtat tttaaataaa
    12721 caactaaaat tgctgatact atgaatccaa catatgctcc cattttagtc gcttttttac
    12781 aaaatgcacc aagtacaaat attcctgcta aaacacctag tactagcccc ataaaactat
    12841 taaaccattc atatgctgac tttatttctc catttgctaa tactattgca acagctatag
    12901 aaagtatacc tacacctaat gatatatatt gtgctatctt agtttgcttt tccatactta
    12961 tatcttttgt tatgatagtt tgtatatcta aagtccaact tgtagcaact gaattaagcc
    13021 ctgtcgataa agttgattga gaagctgcat atatcgctgc aagtagtatt cctgttatac
    13081 ccactggtaa ttcataagct atatatgatg caaatacttg gtcttgttga actgtttgag
    13141 ctaaatctgg gttttgagta tagtatataa ataaacaagt tccaattaga taaaatactg
    13201 ttgcaagtcc cattgataaa acaccatttc catatgtcat tttattaagt tgctttatat
    13261 cagttgttgt agtaaatctt tgaactatgt cttgactcga tacatatgat gaaaaagtat
    13321 taagaccagc tccaacaaat attataaatg cacttgtaga tagtatattt ttatcaaaca
    13381 taacttcatt acttgctaag aacttgtgtc cagttgtaag agtatccatt actgttccaa
    13441 aaccaccatt tatacttgct attaaataga acaatgtccc tgtaacacct actattaata
    13501 ccattccttg tataaaatcg gtccacaaga cagcctttaa tccacctgta tatgagtaaa
    13561 ttattgctat tacacccata actattatca gtacatttac aggtattcct gtaagaccag
    13621 ccagaactat tgatggaaga tacattatga tagacatacg tcctatttga tatattataa
    13681 acatcaaagc tccaagtact ctaagacctt tgctttcgta tctcatttct aagtattcat
    13741 atgctgtatc tatttctaac ctactatata gaggtaagaa aaattttatt gtcaaaggta
    13801 ttgctataaa catacctaat tgtgcaaacc acaaaatcca agtaccacca taagaatttc
    13861 ctgctaatga taaaaatgat attggactta ataatgtggc aaatattgat actgatgtaa
    13921 cccaccacgg aatagttcca tcacctttga agaactcttt tcccttcatt tccttttttg
    13981 aaaataacaa tcctgcaaat aaaactgcca ataagtaaac cactagtatt gcaaaatcta
    14041 tccaagtaaa tcctaccatt tgactaatcc tcccatacta aaagtatttt attaaaaata
    14101 ctttctgtat atttcttttg ctcttgattt catttcatca gttgcttctt tcataggttg
    14161 tctacagtat ccagcctcaa caccttgttc ttcaagtagt aatttaattg tttgatataa
    14221 gccatttcct aatatatctg ttattaaatc attagtcaca tgttgtactt caagtgcttc
    14281 tgatattttt tcattcttag ttaattcgaa tatttgtcta gctctgacac cgtttacatt
    14341 gaatgtagaa ccaattgccc catctacacc taaaactgta gctggtaaca tcatttcatc
    14401 aaatcctgca aatattaatt tatttgggaa tgtttttctc attctctcta ataagtagaa
    14461 atctgctgct gtaaacttaa cacctattat tttttcattt tcaaataatt ctccaaactg
    14521 gtctaaactc atatcaacac cagttaaaaa tggtatagag tatattatta atctattatc
    14581 tacagaattt attattgtat tataatagtg ttttatttct tcaaaatcaa atttatagta
    14641 aaaaggtgta acagcactta ttgcatcata tcctaaatca gttgtaaatt tagcaagttc
    14701 tacagcttct tttaaattaa ctgaaccaac ttgagctatt aattttattt cttctttcac
    14761 ttcatcttta gctatttcaa atattctttt cttttcatca gttgaaagca tgaagttttc
    14821 ccctgtactt cctcctacat aaagaccatc tactttacaa acatctatat tgtgtctaat
    14881 tatttgtctt aacccttttt catttatatt tccttcctta tcaaatgaaa ctaataatgc
    14941 tgaataaatt cccttcatat ctgttgttct catatcttta cctccaattt taattttcaa
    15001 atatagttaa atataataat tttttaattt ataaattaat ttttaattaa tttcaattgc
    15061 tttaacaaat ttttctgtta ttaattgtgg tcttgttatt gctgaaccta ctactgatga
    15121 atgaacaccc aactcaaaga ccttttttaa atcttgagga gtatttacct ttccttcagc
    15181 tattacaggt atttctaaat ctttaactaa tctttccatt aatacaaaat ctggaccttc
    15241 taatgtttta gtatatggag tatatccaga tagtgtagtt gaaacgcaat ctacaccgta
    15301 ctcttgtgct ttgatagcct catcataatt tgaaatatct gccattacta gaacaccgtt
    15361 ttcttttata tactttataa gttcttttaa gtcttcatta tttggtctaa ctctattggt
    15421 agcatctaat gctatcattt cacaccctgt tgttaaaagt tcatcaactt cttttttagt
    15481 tggcgttata tatatatctg aatcttcata gtttctcttt ataataccta taacaggaag
    15541 tccagtaact ttttttattt caataatatc ttcaactcct tgagctctaa ttccaacagc
    15601 tccaccttcc atagcagcct tagccattct ccccattata aaaggactat gaagaggttc
    15661 atcttctaaa gcttggcatg atactattaa tcttcccttt accttatcta gcatttttta
    15721 aattacctcg tttctgaaat ttattttctt gatttatgta ttaataataa catgaaaact
    15781 ttttctttgc aatatttttt catatttttt tctcgcatct atatttttac tgatttattt
    15841 tatatattag cttgtttttt acatttaaaa gaaattttat ttcatttttt tggtgtttat
    15901 tttcatttta tataactact gttataagtc catttaaata atttcaataa aaaaacccat
    15961 gtatactata cataggtttc ccaatattat tctaaatcca ttataatttt tcttccacag
    16021 catggacaaa attcagcctc tgtatctatc tcactttcac aataaccaca aattataaaa
    16081 tcatcacatt cattttccaa ttcaaatttt ttaccacatt cagcacaata tttatcattt
    16141 acatttccaa cataaccaca ctcacaagtt acattacttt ctgccatttc catttcatta
    16201 atttccatat ttttttcata aattaactta tcaagttcta gtaactcatc acagaattta
    16261 tcaaaactat tatcattaat agaatcttct ctaatttttt gatatgtcag tattcccatt
    16321 tccaagaata tctttgcttt cttttcttga atatcatcaa tctctctatt taattttgat
    16381 ttttctttat ataattcaac tttaaatttt ccactaccaa actttttctt aaccttagaa
    16441 aatttatcat ctactttatt attagtattt ccttccacag aaattccctc caatcatatt
    16501 taatcagctg aatttagctt atatcctatt aaagatatca caaaactata tacaaatgaa
    16561 attacaatag cttgtaagac tttaaattgc attattaaag ttgttccata attagttgta
    16621 ttaaagaaat taatattact atctagtata aatgtactaa ataaagccaa tattcccatc
    16681 aaaaatgcat aatatatact aaatactatt ataggtctta tactatctgt attatactta
    16741 aatcttagct tgtatccatt taataataat attaacatag acaatgctcc cattgcataa
    16801 aacattaatt tagtatctcc aaataaagat gaagaactta aagtaaacat tgatactgtt
    16861 gagtttatta tggttactga aattccattt gcaaaagccc acatataact agctatttgt
    16921 gggagtatag taaataaatt taatccattt gaataactac tcatatctag ttcatttaag
    16981 taactactat cagatatagt tagtaccaat agtattacta atactaaaac ataacctaat
    17041 atgaatgttc ttatagaatt tgaaaaaaga gataaataca tattttcctt ttcataactt
    17101 tttttataag tagttatgta tgtacatata aaccctaata caaatccatt taaaagaaca
    17161 ctaaaaaatt cataactata ttctaatgca tatccatatt ctaacatact atgtgaacta
    17221 gtttttacat tagtaaaaat agataaaata gctaaaacta atccataaaa aattccaact
    17281 cctagagaat ttgctaatac agtttttgaa tctctacatc tttttctcat aaaaattagg
    17341 tttgatatta ataataccaa aatagggatt ataagtaata tcaacaaacc tatattggca
    17401 tttataaacc cagaacccat catggtagac atagatacac taatttgacc caagttgaga
    17461 gctaggatta tatgtgctgg atttattaaa taactaatat cattaaattg aatagttata
    17521 atagctttaa atatcaatgc aataataaac aatataaata tggttgtaaa tgttgttaaa
    17581 aatacacctc ttttatttgc atgatatgaa agctctttta ttttagattt caagtctagt
    17641 tttgtttcaa gtggtcttaa tgatgctatt tcataaagtt catttccaca aaatttacag
    17701 taattatcct tagtattatt ttctttattg caactagaac aaattttttc ttctttactt
    17761 ttaagagtga tatggctatt ttttattttt ctagttggtt cacctgtctt tttggaataa
    17821 ttattatgtg aataattcat attatttatt ttcttttcac ccattttttc cctccaagtt
    17881 ttactattgt aaattatcaa atataactat atcatataaa acttaattat accttaattg
    17941 gatatattta tctattttat ttgtttattg ctgttgtttt ttaacaggtc ttgatattta
    18001 aatagtttgt taatgcgaac tttattattt atattttatt atgcttaatt taaatttatt
    18061 ctattcttta ttttcagtag taaactatct gtaaatctta attataataa agtattaatc
    18121 aataaagtta ctcgacatta tctaataaat tttatctatc acaattttag attgttacac
    18181 acaatatatt ttgatataat taaatcataa acgaagtatt ccaaatatct aatgatagaa
    18241 aggaatactt atttttatgc tttattttta atggaggctt atatgaactt agaaaaaata
    18301 aaaaaatttt ttggatttcc tctagacaaa aaacacaaag acaatgttga gaaagaactc
    18361 tgcaattatc tttatcatac gggtaaaatc atttgcataa taatagtaat atctcaaata
    18421 attatgatga tatcaatctc agctagagaa ggaggccctt ttgtcacacc aagacgccaa
    18481 tggtattttt atatgtatgt aacactattt tgtgtatctt ctgtctttat gtttgtattt
    18541 tcttaccttt ggaataaaaa aaaggattgc tgtacgtttt atataaattt atggataatt
    18601 tatgtgactt ttttctgcct atggggttgt gtaattactc tacttgacca gttaggtgga
    18661 aatgacctaa gtgtatttag ttatattatg ttagcagctg cagctttctc agtattaaaa
    18721 tttggagaat cagtaatagt ttttggagga tgctttttgt ttcttaattt attcttacct
    18781 gggattcaaa ctataccagg caatgagttt agtaatattg tcaacacatt ttttataaca
    18841 tcccttgcca tttttgtttc tacaatgcta tatagaaatc gtgtatttac ttactatgat
    18901 aaacttttaa ttagtcaaca atatgaagaa atatctcata taaataattt acttaacgac
    18961 aaagttatga cagatgacct tactaaaatg aacaaccgta ggtaccttga agatattcta
    19021 caaaaaaaat taatcagcca tgcaaatcaa gaacctataa ctggaatgat gatagatata
    19081 gattatttta aacaatttaa tgataattat ggacatcaaa agggagacta ttgtctacaa
    19141 aatattgcca aagtaatttt agattttaca agttctgaaa atgaacatgc tatacgatat
    19201 ggtggtgagg aattttttat atgtattttt aactgtaatg aggcagatgc taaacttaaa
    19261 tcagaatgtt tacgaaaaat tatagagtcc tgcaattttg agcgtgatga tgttccttta
    19321 aaatgtgtaa ctgttagtat tggagtttat acaaagaaaa ttggaaagac aataacatta
    19381 aatgagttta taagtaattg tgataaggcc ctatatactg caaaagaaca aggacgtaat
    19441 tgtgttgcat tttattaaaa ggttacatac tattacataa acctataagt attatatcgc
    19501 aaaataaaag taatcaaatt atatatttat agtacaataa aaataacttg catatctcta
    19561 aaatagataa atatgcaagt cattttagtg tttatactta aagttatata agttttatta
    19621 tatataatta ttaaaattca gcatttttag gagttcttgg gaaaggtata acgtctctta
    19681 tgttagacat acctgtcata tacataatta ttctttcaaa tccaagacca aatccagagt
    19741 gagtagcagt accaaacttt ctaagttcta aataccacca ataatcttct tctcttaatc
    19801 ccatttcatt aattctatct aacaatacat cttctctttc ttctctttga gaacctccta
    19861 cgatttctcc aactccagga actagtaaat ccattgctct aacagtttta ccatcttcat
    19921 tcattctcat atagaaagct tttatatctt ttggataatc tgtaacaaac actggacaat
    19981 tatatatttg ctccgttata tatctttcat gctctgtttg aaggtcacat ccccactcaa
    20041 caggatactc aaattcatgc ccagattcaa ttaataattc cactgcttta gtatacgtta
    20101 ttctaataaa ctcagagttt actatcttat ttaatctttc taacaatcct ttatctataa
    20161 aactattaaa aaattccatt tcttcaggag cattttctaa tacatagctt ataacatatt
    20221 ttatcatatc ttctgcaagt tccatgttat cttcaaggtc tgcaaaaact atttctggct
    20281 ctatcatcca aaattctgaa gcatgtcttc ctgtataaga gttttcagct ctaaatgtag
    20341 gtccaaaagt ataaacgttt ctaaaagaaa gtgccattat ctcggcattt aattgaccac
    20401 taactgttaa attagcctct tttccaaaga aatcttgagt atagtctatt ttgccatctt
    20461 cagtttttgg aatatcattt aaatccatag ttgttattct aaacatctct cctgcacctt
    20521 cacagtcact acctgttatg attggagaat gtgcatatac aaagtttctt tcttggaaga
    20581 atttgtgtat tgcataggca gctattgatc ttactctaaa tacagctgaa aaagtattac
    20641 ttcttggtct taaatgtgct atcgttctta aatattcaag cgtatgtctc ttcttttgta
    20701 gtggatacga actatctgat tccccttcta caactatgct ttttgcatgt atttcgactg
    20761 gttgtttagc cccttctgta gatactaact ctccctctac aagtatagat gagcttattg
    20821 ctaattttcc tacctcttta aaattttcta ctttctcatc aaatactatc tgcatatttt
    20881 taaagaagct tccatcattt agctctataa atccaaaatt tttagatact ctagatgttc
    20941 ttatccatcc agctactttt acagtcttcc ctatgtactg ttctttttct ctgtacaatt
    21001 cttttactgt tataaattcc ttttgcattt taaatcctcc tagtataatt gttattaatt
    21061 tcttttaaat ataatttcaa taaaaaaagc ccttcatccc tatataaagg gacgaaagac
    21121 tattactctc gcggtaccac cctagttgtc ataatgacct cttagttaag caatgcttaa
    21181 agcctataac ggtgccttcc gtctaagtct acttactaaa taagctttcg attagaaact
    21241 ccaagatgtt cttcaatata aacctcgtat tgagctttca ccatcctcaa ctcgctagaa
    21301 ctacatttta tatctactct tctcttcaca gtatttatct tttattattt ctgctaatta
    21361 acaaaattat accaatttat aaatttttag tcaataaatt tatataattg atactgattg
    21421 ttatttaaaa tataataatt ctaccttaca taatgataat aaatacttat cttcattact
    21481 tctttattac ttcaatttcc aaaattttat aatttacttc cattgtcaac ccagtctttt
    21541 gcttcttcga cacccttaat tgaaggtatt gaaactttac tatgaggttt taatttttca
    21601 acatcagccc atgctgctgt cgactcattt ccaactaaaa gtgaactttt aagtctttta
    21661 ttatctttac cttttaatgc tcttgccttt ttactttcca aaactatcac ctctattatt
    21721 tattttgtaa tagttttagc aattttacaa ataaaattca taagtctaaa ttataattat
    21781 ttttaaatta acataatatc ttttcaatac ttaatttata cctatttata tacaaaatat
    21841 ttactttgtg tattcaaaat ataaaactgt tttttaattt tcatatttat tttttatgct
    21901 atttttctac tctatctaaa gtatttgata caattaaact aataatcaat tataatttta
    21961 cataggttct tatctataga gtatttgcta tctgttgaat cgtatttttt aaatttaaaa
    22021 tcccatatcc ctgcgataca tttggatatg tatatatctc aagttttgta gcacccaata
    22081 ttaaatatgt ttttaaaact tgtgtaaaca atgataatct cggtacatta tcctgttttt
    22141 ctaagtattc catcaataag gctaaaacac ctgtgactat agaactgctc actcctgttc
    22201 ctgttcctgt attatatgtt ccatttttat atgtggatat tatatctaca cctgaagcta
    22261 ctatatctgg cttaattcct cttcctctaa ttggtccttt cgaagaacca atccacatac
    22321 tatcagtctt attgttaaat gtaccaactg taattacatc atcactagca gcatacatag
    22381 ttatagtagc tactgaatca ggctctaaaa accttgtgtc tggagctatc aaatttttat
    22441 ttggcaagta tatgtcatat tctccattta ttataagttc tgaagtcaat cttaaaatcc
    22501 atactccagg ttttatatct cttaatctta tctccaaatt ttcttttcca gaggtaatat
    22561 atggataaat aaatctcatt gcataagtag tattttctaa gttaaatttt cctctatata
    22621 tataaaagtc tggagaatac cttatatcat gactaacttc acctgaagga gatattatct
    22681 gtgctcccac tttatctgga ccattcgtat ttagtgttat atcaagagca tagtcatctc
    22741 catcttgtat tattacatct tgaacttctc ccacactgct aaatctgcca gaataatgaa
    22801 tatctgtatt cccttgattt cctgcaccac tcactactac cacaccagca ctactcaata
    22861 tattaaaagt atctagtatt gaagtagttg ctacagcact tgacttaact ccaatagtca
    22921 aatttattat cagtggttta ttttctgttc ttgctatgtt agtaacatac gttatagctg
    22981 ctaaaaaatc tgaaacactg taatttatcc ttccagcata ataagtatct gtatatgatt
    23041 ttaatttaac tactattaag tctgattctg tagtaatacc tctgtactga gaatttatcc
    23101 ttccatttcc aactaatata ccagaaacta aagttccagt acctatatta tcttgactca
    23161 aactcccatc atttctattt atggctatat ttaactcact tcttgtaaat tcacttccaa
    23221 aaataaaacc ttctggaggt ggatttgtat ttgcttcttg gtcccataaa tataaaactt
    23281 tactagttcc atcatcattt ataaaatcag gatgcaaata atctatccca gaatctataa
    23341 ctgctaataa aattcctctc cctgttatat cattatatgg attttcataa atataatcag
    23401 tttcagctgc tgttgtaatg gtttctccat tatttacatt attagttatt tcaattagac
    23461 tgctcattgg ttcagattct tcccaccaag caacttgtat tatattattt aaaatagttt
    23521 catcaaaatc taaaggtaca tatattactg caagctgact atttaaaacc atatacctat
    23581 ttattccatt ttcttgtagg gcagattcta tatcaccttg ataaattata caataagatt
    23641 tttccataaa tccctcctat cttaaaacat caaacattcc tctaacatcc aagagcccat
    23701 atccagaatt tgtatttgga tatactgtgt ttgaatcttt tctagcacca gcttgcataa
    23761 aagtttttat tttttgcaca tatgcttggt ttggatatct accgtcaaca aaagtatatt
    23821 gaaaatacat tgcagcagca ccagctgcat gtgcacttgc tgctgctgta ccagttatag
    23881 ttgcatacgt gttcccagga taagcagcta ttatattgac acctggtgct actatatctg
    23941 gttttaacct gtcttcaata gttggccctc ttgatgaact ttgccataaa ctaccattaa
    24001 tggtattgta ggctcctatg gttattaaat catcttgaat cgcaggataa tttatagtgt
    24061 aaaaagggtc tacctctcta aatctagttc cactttttaa aagttctcta tttggaaggt
    24121 ataagttata tcttcctgtt ataatataaa ctcctactaa tcgaatcttc catacacctc
    24181 tttttgcatt ctttaaagta acattagtaa attgctgtcc agaaaaagtt gttgggtata
    24241 tatatgttat agaatattca gtcccctcta aatcaaataa acctgttact ttattataat
    24301 ttgaaattcc aacactttta ctttcttcac cagtgggaga tactattatt acatcagctt
    24361 tatctggtct atttaaccaa agttctaatg ataattcttc ctcgtcttca tttaactcta
    24421 attcaacttc aacagaacct ccaacatatg gtattattcc agatgtatgt gtctgtgtat
    24481 tgccctcatt tcctgctcct gccgttatac ataaccctct agtaaagaac gctttttcac
    24541 tattactcct attggttaaa cctgctaggc tactggtacc taaagacata tttataacta
    24601 atggtcttcc aagttcaaaa gcttttttat atgcatactg agatgcagca aacaacattg
    24661 cactattata aaaatcatct atcttgccga gttttattat tattagctca gaatcttctg
    24721 ctatacctgc atattcacta tttacatttc caagccctgc acatatccca ctcaacatag
    24781 ttccttgacc aacttcatcc tgagatagac tagggtcatt ttctgcgata gctcgattta
    24841 tatcttctcg tgtatattct gtcccaatat aaaaaccatc aggaggagtt ccttcttttg
    24901 tttggtccca taaatatact atttttgaag tgccatctgg ataaataaaa tctgggtgta
    24961 gatagtctat tcctgtatct gcaattgata ttagagtccc tcttcctgtt atattaatat
    25021 taggattatt tttaaagaaa tttacgccca tttcttctgt tgcaactaca cctccaaaag
    25081 tacctcgatt aatttctcca agtaatgtca tctttgtagt cgatacagtt cttatgattg
    25141 aaggaagttg caaaactcgt tgtatgctat tatagtctgt attatttata gatagtacac
    25201 ctaatgaatc acttatctta aaaaaccttt gactcatacc taacccagct agttcttctt
    25261 caaattcagg agtatggact atatctattc ctgatagtat tcctgattgt ctacttgcat
    25321 ttttacttat ataaatttca ttctctaagt cattatgtat ttgcatatta taaccatcta
    25381 taaacttata actattttcc tcatctttaa atgacttatt aatacttcta tatccaatat
    25441 cctctgtttc taaatcttga tttatattag atagagtgta caaatctata tctgataaat
    25501 ttaaaaaacc aaatcctgat gaattatttg gataagattg attacttaat ctccttgctc
    25561 cctttagaag taatgccctt aatttctgtg aatacaaaaa taaatcattt ccatttacaa
    25621 ttccccattc cataaacaat gaacaaaccc ctgtaacatg aggtgtagcc atgctagttc
    25681 cagttaatgc tccacttgta cctcctggta aaaaagatac tatatcttca cctggagcta
    25741 ataaatcagg tttaaaaaca cctaattgtg tatctccttc accagaaaat atagatacta
    25801 tatctgttct tgaattaaaa ctacctactg ttataactct acttgctgtt ccaggaactg
    25861 ttacagtaag ctcttgagta ggaatcagaa atctagtatt tctatttaga ccttcagaag
    25921 ttggcaaata tatatttaca ttaccagtta ctatatctat tggttcaaat acaattttcc
    25981 ataacccagg agttatctgt gtatttgaac ttaattgtag agtaactctt cttgtgagag
    26041 aatacggtgc tatagggtaa aaataaccag ttattctagt ttcacccaaa gtattcctta
    26101 tctcacctga agttagagag attgcttgtg tttgattatt tgatgggttt accaaatgta
    26161 cactaaaatc atcaacaaaa tctggccata tatttatgtt taatatacgc tctccttccc
    26221 caactataaa ctcaacttct tctgtaatat tattttgaag cctaatcctt ttatgacctc
    26281 ctttatctgc attatttcca gctgctacaa ctatattgtt tttccaaaat aaacacatat
    26341 catctatata ttgttcaaat aaagatgttc ctctatgtga accttcatta cttccataac
    26401 ttatatttaa tgtaacaggc attcttaatt ctaatgccct atctaaaata aatttaattg
    26461 ccctcataaa ctctgtactt ctcgaaaaaa tatcggtctg tatatttcca actctaacca
    26521 ctattattct ggcatcactt gcaatagttg cacaaatacc tgcaacatgt gttccatgta
    26581 agctagttgt tgaaataggt atatacatac ttccatctat tgcattattt atatcttcat
    26641 tagtatataa tgttccttct ctaaaacctt ctggtggatt tccttgaata gattggtccc
    26701 aataatataa tatcttagac cttccatcac tatctctaaa cacaggtaga gtataatcta
    26761 ttcccgaatc aattatacca attatagttc cttttccagt taaaccagtt ctatttttaa
    26821 atcctgttat tcctgtactt gaaaaacttt gcacatcctg agtctgtaat ataaaaggct
    26881 tttctataaa ttcaatttct ggataagtta aaagtatatt tacatcttct tcattacttg
    26941 atgttattat tgcatatgat gaattaagta tctctactga tacacctaat tcttcttcta
    27001 acctcaatat atctccattg tattttacaa ttaattcata atttattata atcacctcga
    27061 cttctaaaat tattattaat gtatatgttt tgtgtgagtc taaaatgtat tagactatat
    27121 aattttttaa gtttcagtta tacaatttat aatagatata ttaaaaaaat aaataatgat
    27181 ggtacaattt tgaataaatc ataccatcat ttatatttac tttatcttga aaccattaaa
    27241 tttttcaatg ctttctcaag accttttaca gtcaatggaa acatattaaa ttcactttgt
    27301 atcattcttt gagtttgata ccaataacta attgaatccc atccatctct aagttctgga
    27361 ttcatccata caattttact gtactttctc ttaaaccttt taagccactc tataccaggt
    27421 gtataattat atggaccacg atagttacca ccaatatgaa gcaactcaga aggcgacata
    27481 gaagcatctc caacaattat aactttatag tttttatcta tattttttaa tatccattca
    27541 gtattaatag atttacttaa ccaacattca ggtgttgtaa ataatctatc ataaatacag
    27601 ttatggaaat aatatatctt tacatcttta aaatgatttg atttacttac agactgaaat
    27661 aatgtattac ataaagtgct ataccctctc attgaaccac ctgaatccat aagcaataaa
    27721 agcttcactg tattttttct tggcttctca aaaaataact ttaaatatcc tgcattatta
    27781 caagtctctt caatagtctt atccaaatca agctctgtct ttggagcatc tattctagta
    27841 gaaaattgac gaagtctctt tagtgcaact tgaaattgac gcatatttaa aacttcatca
    27901 tctctaaaat ctctgtattt tctttctcct gcaacttcaa gaacagctct accataagta
    27961 gaatttccac ctatacgtat accagtttgc ccttttcctg aatgtcctaa ctctgaagtg
    28021 cctcctgtgc ctatccaata attaccacca ttatgaggag aatcttgttc ttttattcgt
    28081 tcctccaatt tagctcttaa tttatcaaga tttatatttg gattttgttc taatctttca
    28141 aactcttcta aatctatatc tggtttttta agccaattca taatctcttc tggaatttct
    28201 tcttggtgtt ctataccttt aaaatactcc aaaaacactg catcaaactt atcaaaatca
    28261 ctttcacttt ttattaaaat catcctgcaa agatagtaaa aatttgaaaa gtttgaatag
    28321 cacagtcctt tatccaatgc ttcaattaaa gtcatccatt catttaaaga tacatttaat
    28381 cctcttgacc tcaataaata aaaaaattca ataaacataa ttatccagct cctattaact
    28441 ataagtgtct ttgcatagat tcaatatcct cattattctt aagtagtatt cctgcaaaag
    28501 gaacttcttt ttctattttt tcaataggca ttccactcaa agttaatgct tgtatccagt
    28561 ctattaactc tgatgtacta ggcttttttt gtatgtcctt taaacttcta atccaataaa
    28621 aagtagtcat tacttgttcc aataggtgtt cttctacttt atcaaaatgt actttaacta
    28681 tttcttccat catgtctcta tcaggaaatt ctatataatg aaatatgcat cttcttaaaa
    28741 aagcatctgg aagttctttt tccgcatttg aagttataat aactattggt ctttgtttag
    28801 ctcttacagt ttcttttgtc tcatttatgt aaaactccat tttatccaat tcccaaagca
    28861 agtcatttgg aaattctaaa tccgccttat ctatttcatc tattaaaaga attacttgtt
    28921 gatttgagct aaaggcctct cctagtttac catattttat gtacttgcta atatcatcta
    28981 caccttctcc tccaaattgg ctatcataaa gtctttgaac agtatcatat acataaagcc
    29041 cttcttgtgc ttttgtagtt gattttatat tccaaattac taaatctttt tttaattcat
    29101 ttgaaattgc ttgtgctagc atagttttac ctgttccagg ttcgccctta atcaaaagtg
    29161 gcttttcaag agcaatagca acattaactg atgccatcaa ttctggtgat actacataat
    29221 cactacttcc tctaaagtta gatatctctt tttttatcat aaatcctact ccttttaaac
    29281 agtattttct aaatgtatat agactttatt actatttttg atataaaaat aataatacaa
    29341 tacatttgta tagtattatt ctaaaattct atatctaaat aaaatcacct ctaaaatatt
    29401 taattttta
//
LOCUS       DAELTA010000034        28743 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.034, whole genome shotgun sequence.
ACCESSION   DAELTA010000034 DAELTA010000000
VERSION     DAELTA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.034"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            15..1733
                     /locus_tag="KPG13_001291"
     CDS             15..1733
                     /locus_tag="KPG13_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0985028.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            1764..3764
                     /locus_tag="KPG13_001292"
     CDS             1764..3764
                     /locus_tag="KPG13_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985029.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            4115..4360
                     /locus_tag="KPG13_001293"
     CDS             4115..4360
                     /locus_tag="KPG13_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG0985030.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            4395..5120
                     /locus_tag="KPG13_001294"
     CDS             4395..5120
                     /locus_tag="KPG13_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG0985031.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            5142..6455
                     /locus_tag="KPG13_001295"
     CDS             5142..6455
                     /locus_tag="KPG13_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0985032.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            6661..7200
                     /locus_tag="KPG13_001296"
     CDS             6661..7200
                     /locus_tag="KPG13_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0985033.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            7289..9274
                     /locus_tag="KPG13_001297"
     CDS             7289..9274
                     /locus_tag="KPG13_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG0985034.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            9498..10466
                     /gene="spoIIIAA"
                     /locus_tag="KPG13_001298"
     CDS             9498..10466
                     /gene="spoIIIAA"
                     /locus_tag="KPG13_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG0985035.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            10456..10977
                     /locus_tag="KPG13_001299"
     CDS             10456..10977
                     /locus_tag="KPG13_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG0985036.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            10994..11191
                     /gene="spoIIIAC"
                     /locus_tag="KPG13_001300"
     CDS             10994..11191
                     /gene="spoIIIAC"
                     /locus_tag="KPG13_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG0985037.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            11207..11593
                     /gene="spoIIIAD"
                     /locus_tag="KPG13_001301"
     CDS             11207..11593
                     /gene="spoIIIAD"
                     /locus_tag="KPG13_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG0985038.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            11678..12883
                     /gene="spoIIIAE"
                     /locus_tag="KPG13_001302"
     CDS             11678..12883
                     /gene="spoIIIAE"
                     /locus_tag="KPG13_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG0985039.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            12943..13560
                     /locus_tag="KPG13_001303"
     CDS             12943..13560
                     /locus_tag="KPG13_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG0985040.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            13572..14126
                     /locus_tag="KPG13_001304"
     CDS             13572..14126
                     /locus_tag="KPG13_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG0985041.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            14153..14842
                     /locus_tag="KPG13_001305"
     CDS             14153..14842
                     /locus_tag="KPG13_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG0985042.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            14952..15314
                     /locus_tag="KPG13_001306"
     CDS             14952..15314
                     /locus_tag="KPG13_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG0985043.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            15399..15905
                     /gene="nusB"
                     /locus_tag="KPG13_001307"
     CDS             15399..15905
                     /gene="nusB"
                     /locus_tag="KPG13_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG0985044.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            15889..16959
                     /locus_tag="KPG13_001308"
     CDS             15889..16959
                     /locus_tag="KPG13_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG0985045.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            16956..18161
                     /locus_tag="KPG13_001309"
     CDS             16956..18161
                     /locus_tag="KPG13_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG0985046.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            18163..18378
                     /gene="xseB"
                     /locus_tag="KPG13_001310"
     CDS             18163..18378
                     /gene="xseB"
                     /locus_tag="KPG13_001310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG0985047.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            18381..19268
                     /locus_tag="KPG13_001311"
     CDS             18381..19268
                     /locus_tag="KPG13_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG0985048.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            19298..19735
                     /locus_tag="KPG13_001312"
     CDS             19298..19735
                     /locus_tag="KPG13_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG0985049.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            19769..21634
                     /locus_tag="KPG13_001313"
     CDS             19769..21634
                     /locus_tag="KPG13_001313"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG0985050.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            21767..22579
                     /locus_tag="KPG13_001314"
     CDS             21767..22579
                     /locus_tag="KPG13_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG0985051.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            22599..24278
                     /gene="recN"
                     /locus_tag="KPG13_001315"
     CDS             22599..24278
                     /gene="recN"
                     /locus_tag="KPG13_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG0985052.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            24492..25358
                     /locus_tag="KPG13_001316"
     CDS             24492..25358
                     /locus_tag="KPG13_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985053.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(25451..25942)
                     /locus_tag="KPG13_001317"
     CDS             complement(25451..25942)
                     /locus_tag="KPG13_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0985054.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(26003..26443)
                     /locus_tag="KPG13_001318"
     CDS             complement(26003..26443)
                     /locus_tag="KPG13_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0985055.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            26604..27662
                     /locus_tag="KPG13_001319"
     CDS             26604..27662
                     /locus_tag="KPG13_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0985056.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            27804..28628
                     /gene="spo0A"
                     /locus_tag="KPG13_001320"
     CDS             27804..28628
                     /gene="spo0A"
                     /locus_tag="KPG13_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG0985057.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
BASE COUNT        11785 a         2833 c         5222 g         8903 t
ORIGIN      
        1 ggaggactaa aattttgaat tttagactac caaagaaaag gttacccaaa aagataattt
       61 ttatatgtat aatacttatt agtattattt tgtttataaa gtatgaccaa aatggattga
      121 aagaaagaga aattataaat aagggaaaat atatgtctga aagccttgtg gcagataagg
      181 agtatgatga agcaaaaaaa gtggttaaga cttcttttga atctattcca gaaaaaaaat
      241 acaataaagt atataatgaa gtttgggata tgttaaagac tatttcatat gttccagatg
      301 gaacgaagat agtcattgat tctttagaaa agatgagaga atcagatgtg aaattatctg
      361 atgaatgtag atttaatata gaaaaaagat tagcatcagt gtatataatg gataataatt
      421 attctaaggc agtagattta actgtaaagt ctataaagtt ggctgagaaa ttggggaata
      481 attatgaaaa agcaagacta gatgttgatt tggcgagcat atttttaaag ataggtggct
      541 atgaaacagg agagaaatta ataaaggatt cttttaaaat agatattgat gaaggtgaaa
      601 aaaatggaat ggttagatta tatgctatta taaatctagc agaaatatat gtagaagagg
      661 gcgaatatga taaagctatt gaaataagta gcaggataaa agattataaa aagtttgtaa
      721 atacggataa ttacaatgat tttgatataa tggcctctat aatgcagtca aatgcgtatg
      781 tgggcaaaaa taatatacaa aaggcaaaga cttttttaga aaaagctgat tatcttataa
      841 aatcagatag aacagtttat atattagata aagatatgta ctattatatg gcaatgggaa
      901 acttagaata taaaagcgaa aattataatt ctgcaataga taattataaa aaaagtttag
      961 agatatctac taaaagaaag ttaacccagg agaaaataaa aaatttaaat aagatattaa
     1021 atatatatga aaaacaagga aatatagaat ctttaaatga gtatcaaaag atgttaataa
     1081 atgaatataa agagaacaaa agtataagag attcagagac tagtttctat ataattgata
     1141 aagtatcaaa cgaaagtaag ctatttgaaa aaactaaaaa agaaataaaa tattataaaa
     1201 tattatttac agtaatttta attgctatat tagggtctac ttttttatac aaccgattga
     1261 agtattttaa agcacaaaat ttacatgatg gtcttacaaa tgtttataat agaaaaagtt
     1321 ttgatattat gtatgaaaag tacagagaga aagatgataa ttttgcactt gtgatgatag
     1381 atattgataa tttcaagctc ataaatgata attatggaca taaatttggt gacactgtta
     1441 taaagggaat aacaaatacc atttgtgtta tgttagaaca agatgataaa gtctttaggt
     1501 acggaggaga agaattttct gtattaataa gaaataaatc tggtgatgaa gtagcagata
     1561 taattgacag tataagggtt gcaattgcaa ataaaaagtg gaatgatgat gtgactgtga
     1621 ctataagtgc aggtgtagct catgtttcag atagcaaaga tgtactagaa gaagctgata
     1681 aaaatttata taaagcaaaa caacttggta gaaataaagt agtatataag taattatata
     1741 ctatataaat aagaggtata aatatgaaac aatcaatagc tatagtaact gataaaataa
     1801 ctaaactatc atctttctta gaagaaaata tacgtttggt tttaggtaat tatattgata
     1861 taaaccacta ttatctggaa gttttaaaag agaatgataa aatacaaggt gattttgtac
     1921 tagtaatgaa tgatgataga ttaaacagta tgagaaagca tttagtacaa gatagtaaga
     1981 ttatagtgat aaggagaaca cttaaagaaa atgaaatata tggattattt tcaataccaa
     2041 atggaactga agtattggtt gtaaatgata caaaagaaac taccttagaa acgataagct
     2101 tattttacaa aataggagta aaaaatttaa agtttactcc atatattgaa ggaaaaattt
     2161 ataaaaatat agacatagct ataacacctg gagtaaaggg aaaagtacca agttatataa
     2221 ataaagtaat cgaccttgga aatagatata ttgatatatc tacctttata gaaataataa
     2281 atggtcttag aatagatgtt aaagaaatac gtaaaaattt aatggcatat tctgagagtt
     2341 taataagttt agataatgga gttaaagata attataggca actgttttta aaaatagaag
     2401 aacaagatgt tatattgaat ctatcaaaag atggaataat atttacttca gcagatggta
     2461 taataaacac atttaataaa gaagctttaa aaattttagg tataaatgaa aatccacaag
     2521 gcaaaaacat agaagatatc atttctgaag atttaaatat tttacttgaa gacaaagaaa
     2581 taattgatga agtggtattt acaaataaaa aatatataaa tgtaaataaa aaaagtattt
     2641 tttctatggg gaataaagct gggatatatt ataacttaca agaaataacg tatataaaaa
     2701 aattagaaca aaatcttact aataaattaa gagaacaagg tcagatagca agatatacat
     2761 ttaaagatat aaaaactaaa aataaaaata tggtaaagtg tgtagaattg gctaaaaaaa
     2821 tatctaaatc agacctttct gtattaataa taggagaaag tggtactggt aaagaattat
     2881 tatcacaatc aattcataat gcttccaata gaagtaatca accgtttata gcagttaatt
     2941 gtgcagcagt tccagatagt ctattagaaa gtcaactgtt tggatatgaa aaaggctctt
     3001 ttacaggagc tttaagagaa ggaaaaaagg gtctatttga acttgctaat aatggaacta
     3061 tatttctgga tgaaattgga gatatgcctc cattgctaca gacaaagtta ctaagggtat
     3121 tacaagaaaa gcaggttatg cctataggtt cccataatgt tataaacata gatgttaggg
     3181 tcatagcagc aacaaacaaa aacttactaa agatgattag agaaggaaaa tttagagagg
     3241 acctgtatta tagactaaat gtattgccaa tagatgtacc atcattgagg aatagaaaag
     3301 aagatataat aacacttatg aatttctttt tgaaaaacaa tataagactt tcgccagaag
     3361 ttataactat tttagaaaaa tataattggc caggaaatat aagagagtta caaaatgttg
     3421 cttcttatgt aagtctaatg tgtgatagta tggtttttag agatgactta ccaccatata
     3481 taagggtaga atgtgctaac aattatgaag atggatttgg ttatacaaga aaagaatttg
     3541 aggaaatact gaggattcta tatgaaagaa gagagtcatc ctctggaatt ggaagagggt
     3601 ttataatcga aaagcttata caggaaggta ttgagatttc tgaaagcaaa gttagaaagg
     3661 tgctatctta tttaagtgaa tgtggataca tatcatctaa atctggtaga ggaggaagtc
     3721 aaataacctc agaggggata aaactttata ataatataaa ttaagctaag tatttgatat
     3781 tgaaatcaca tattttgaat ttagatattt aaataataat aaacttttat atttatagag
     3841 attaatttat attatgtcag aaagaatatt gtaatttata attaaatatt aataggtttt
     3901 taaaatagga ttagataggt aataggttaa gttagaataa cccattacct atttttattt
     3961 gtaagaaaag tgtacataga attagaagta aaaaatacaa ctaataaaac tattgaaaat
     4021 actagataaa taataaataa aaataattat agattttggc atacatattg ctaattatag
     4081 agataagacg acaaaataat tgggaggttt aatgatgaaa tacaaaacaa cagggacttg
     4141 tgctacagaa attgaatttg aagtaaaaga aaataaagtt acaaatgtaa actttatagg
     4201 tggatgtgat ggaaatttaa aagggctaaa agtattagtt gaaggcatga atatagaaga
     4261 tgtaatacaa aagttaaaag gaatagagtg taaaacaaag ccaacatctt gcccagacca
     4321 actgtctcta gcattggaaa attatatgaa tataaagtaa tacataaaac aatcgattag
     4381 ttaggagttt aattatgaaa ccaataatag ggattttagg aaatttaata ataatggaaa
     4441 atggtatgtt tccaggtctt gaaaggtcat atgtaaataa tgattatata aatgcagtgc
     4501 taaaaggtgg cggtagtcca gtaataattc ctgtaaatac agataaagaa gttataaaaa
     4561 aacagattga aatggttgat ggagtcttaa tatctggggg atgggatatc aatcctcaac
     4621 tgtatggaga agaaacaaga gaagaaacga catttatata tccagaagtg gatgaatttg
     4681 atttaattgc aataagtata gcattagaac ttaaaaagcc tatattaggt gtatgtaggg
     4741 gactacaaat tttaaatgta agtttaggag gaactttata tcaagataat aatcttatag
     4801 aaggaagtta tataaaacat acacaatctt ctaaaagaca tgtagctact cataaagttg
     4861 atgttaaaga aggaagtatt ttagagggaa ttttaggaaa acaattactt acaaatagtt
     4921 atcatcatca aagtgtcaat cagcttggaa aaggattaaa agccatagca tactcaaaag
     4981 atggtataat agaagctatt gagaaagagg atgaaaattt tgtagtagga gtacagtggc
     5041 atccagaaat gatggtagat tactgtgata aaatggaaaa actatttaaa tattttataa
     5101 atatttgttc gaatttataa tataccttgg ggggattgaa catgaacgaa aagaacaaaa
     5161 tgggattaat aagtattata cttttaggta ttaatgcagt agtaggagct ggagtttttc
     5221 tattaccagg agatgcaatg aaatcatttg gtgtagcaag tatatttgta tatatatttg
     5281 atatgttgtt agttttatct atggcattct gttttgctga ggttgcaggt aaattcaata
     5341 aaaatggtgc tgcctatgta tatacaaaag aagcttttgg agatttttgt ggttttgaag
     5401 taggtcttat gaaatgggta ataggatgta tttcttgggg agctttaata gtaggttttc
     5461 ctacatcatt atcagcagta tgggctccag caggtgaacc acatatacaa aaaattataa
     5521 ttgtagctat gatagttggg cttacaatta taaacttatt aggtgtatct ctatcaaaaa
     5581 ttgtacaaaa tgttataact gtaggtaaac taattccatt aatactattt ataggaatag
     5641 gtatattttt tataaaagga gttaatttta cttcttcaac aatggtacct ccaggagctg
     5701 gagcaacaga gtttggagca gcagcacttt taatgtttta ttcatttaca ggatttgaat
     5761 ctatagcagt tgcagcagaa gatatggaaa atccacaaaa aaatatacca atagcaatta
     5821 taagtgtaat agtaattgca tcaataatat atatattaaa tcaagttgta tgtgttggta
     5881 tactaggaga ttcgttatct agtacatcta caccagtggc agatgctgca agaatatgct
     5941 ttggaaatat gggagctgga ttggttacct ttggaacatt ggtttctgtt ggtggaattt
     6001 gtatgtgtgg tgcttttgta aatccaagaa gttgtgtagc tttagctgat gataaaatgt
     6061 taccgagaat atttgcaaga aaagataaaa aaggtacacc atatgtagcg ataatagcta
     6121 ctatgttaat cactatacca atagctctat ctggaagttt tgctgaatta gcagcaataa
     6181 gtgcagttgc tagattcata caatacatac caacatcttt atccgtatta gtgtttagaa
     6241 agaaaagacc agaattagta ggtactttta aaactccttt tggagcagtg attcctctaa
     6301 ttgctgtatg tgttggaaca tggttgttat tccaagcatc tatgcatcaa ttaattatgg
     6361 gattaggagc tttggcaatt ggagtaccat tatactttat aatgaagtct tataatagaa
     6421 aaatctatgg tgaaaattta aagaaaatag tttaattatg ttataacaat ttattgagtt
     6481 aaaattagac attggcagaa aattaaatac aaataagtat ctacaaggct atattaaaat
     6541 attattaatt ttttatttta atatagcctt atattgttat caataaatga aaaatattgt
     6601 aacaacagaa gaacaatagt attatgttta tagacaaaaa acttattagg aggtaaattt
     6661 atgccaacaa taacattaaa aaatggtgta gacgtactta ttagagaagg tgtaagagaa
     6721 gatgctcaaa gcattataga tttttataat gaagtgggtg gagaaactca ttttctgtca
     6781 tttggaaagg atgaatataa aatatctttg gaggaacaag aaaatgctat agaatcagca
     6841 aaagcatctg ataattctgt taaactgatt gcatttatag atggtgaaat agttgggatt
     6901 gcaacgattg attctaatca aaaagcaaaa ggaaagcatg ttggagtatt aggaattgtt
     6961 gtaaaagaaa aatattgggg aattggattg ggtaaaaggc ttatgttaga ccttatagaa
     7021 tggtgtaaat caaatggaat aaccaagaag ataactcttg tcactaatga agaaaattat
     7081 aatgctatag gattatacaa aaaagttgga tttgaagtag aaagcatact aaagaaagaa
     7141 tgttattata atggtgttta tactgattta ataggaatgt cacttttatt gggaatataa
     7201 ttagtgtaca ttttatctcg aaaaaagtat attaagtatg gtataattaa aaaagaatat
     7261 aacttaatat aattttggag gacgaattat gaaaaatctg tgtaaaatat cagatgatta
     7321 tttaaacagc aagcttaagt atttaaagtt attatcaaaa caatatccaa gtatatcaaa
     7381 agcaagtaca gaaataataa atttagaggc tatattaaat cttccaaagg gaacagagca
     7441 ttttataaca gatgtacatg gtgaatatga accatttgta catgtattaa aaaatggttc
     7501 tggtgtaata aagagaaaaa tagaagaatt gttttcaaat acaataagag acagcgaaaa
     7561 aaaaatgtta gcaactcttg tatattatcc tgaacaaaag ttagacctaa taattaagca
     7621 agaggaaaat atagatgatt tttatagaat aaatatttat agacttatag aactttgtaa
     7681 atatgcttct agcaaatata ctaggtctaa agttagaaaa ctgttaccag aaaattttaa
     7741 atacataata gaagaacttt tgcatgagca tgttaaaagt gaacataaag aagagtatta
     7801 taaaagtata gttgaaacaa tagttgacat aggaatagct aaggagttta taattgctat
     7861 ttctacagtt atacaaaaat tggttgtaga tagacttcat gtaataggag atatttatga
     7921 tagaggtcca agaccagata taattgtcga taagcttatt gaacatcatt gtgtagatat
     7981 tcaatgggga aatcatgata tattgtggat gggtgcagcc tcaggtgaaa aaacttgtat
     8041 agcaaatgca ctgaggatat cagctagata tgcgaattta gacattgtag aagacatata
     8101 tggaataaat ctattgcctt tagccacttt tgctatagaa atgtataaag atgacccttg
     8161 taaagaattt attccaaagg ttaatgacca aagtgtaaca acaacagaaa agtctttaat
     8221 ggcaaaaatg cacaaagcaa taagtataat tcaatttaag cttgaaggtg aggttatcag
     8281 acgaagacct gaatttgaaa tggaacatag actcttgctt aatatgataa actatgatga
     8341 aggtacgatt aatttaaaag gtaaaacata taaattaaaa gatacttatt tacctacaat
     8401 agacaaaaaa gacccatata aattaactat ggaagaaaga aatgttattg ataaattagt
     8461 atcttcattt agaggtagtg aaaaactaca aaaacatgtt tcatttttgt tttctaaagg
     8521 aagtatatat ttaaaggcta actcaaattt actgattcat ggatgtgtac cgttaaatga
     8581 agatggaagc tttatgtcta tgaatataat gggaaaagag tataaaggta aggctcttat
     8641 ggataagatg gagtctctag caagagaagg atttttcttt aaagataagg cagaagaaaa
     8701 actgtatggc atggatatta tgtggtattt atggactggg aaatgttcat cattatttgg
     8761 taaagatgat atgactacat ttgaaagata ctttatagct gaaaaagaga ctcataaaga
     8821 aaataagaac ccatatttta aacttagaga aaatgagatg gcttgtaaaa gattatttga
     8881 agaatttgat ttagaacttg atgaatctca tataataaat ggtcatgttc ctgtggagag
     8941 taaaaatgga gagagcccaa taaaagctaa tggtaaaatt ctcgttatag atggtggttt
     9001 ttctagggct taccaaaaaa ctactggtat agctggatac actttgatat acaattctag
     9061 aactttgcaa ttagtatcgc atgaaccatt taattctgct gaagaagcca ttgcaaatga
     9121 aagtgatatc ttatcgacaa ctgttgtagt agagcataag gcaaagagaa aaatggtgag
     9181 agatactgat gaaggaataa agatacaaga ggaaatagaa gatttaaaac tacttttaat
     9241 ggcttataag aaagggctaa taaaagaaat gtagatgtta tagaggcact tcatgattga
     9301 agtgtctttt tgttaatact tattaaatat attataaaga ttaataatag tagtttatat
     9361 tatacttact aagtgaatcc aagataaatg ggttcacttt ttttatttat aaacattatt
     9421 aaaaaaatat agatgttttg gttatattcc ttgtccttta taaatagaat tatataaaca
     9481 aaggaggagg acaagagatg aataaacttt ctgatgaaat aataaactct ctttctacaa
     9541 ctataagaga aaaggtagaa aaagtctcaa acaataatct gaacatagaa gaaattaggt
     9601 tacgctcaca aaaaccattg atactaaatg caaactctaa agattatttt tacaatcaaa
     9661 aaactatgac acttgattta aatcaacaaa actcatatgt agtaacaaga gaagatgtag
     9721 agcaaacttt tcaaataata tgtaaatatt caatacattc atttatggat gacataaaaa
     9781 aaggattcat aactttgaga ggaggtcata gagtagggtt agttggaaaa gcaatagtag
     9841 aagatggaca agtcaaaaat ataaagcata tatcatcttt aaatataagg gtatcaaggg
     9901 aaattattgg atgctcggat aagattttaa gtcatatcat aaagggaaaa aatcagatta
     9961 ataatacttt gataatatca ccacctcaat gtggaaaaac aactttaata agagatatag
    10021 taagaaattt aagtaatggt aatgaagatt atggatttaa aggtttaaaa gtggctttag
    10081 tagatgagcg taatgagata gcaggagctt atctaggtgt tccacaaatg gatgtgggaa
    10141 taaggactga tataatagaa acttgcccaa aagacttggg tattacaatg cttttaaggt
    10201 ctatgtcacc aaatgttatt gtgacagatg aaattggaag tgaaaaagaa ataaaggcat
    10261 tatacacagc attaaatgga ggtataggat taataactac tgtgcatgga gattcaatag
    10321 aggatataca aaatagaaaa gaattaaata gacttctgga taaagagtta tttaaaaagg
    10381 ttataattct ttctgcaaaa agaggagcag gaacaattga aaaaatttat gatttagaag
    10441 aaaagagatg gtattttgca aattaaaata attattatag ctttcttgat aggaagtagt
    10501 tatttgatag gtgaacaaat atacaaaact tatactcgta gacataaaca attaaacgat
    10561 ttaataagag ttttagaaat tttgaggatg gatttatctt ttggacttta tacattagaa
    10621 gaaatcttta ataggatagg tggaaataaa gagttttgtt tttggaaatt tttctatcaa
    10681 atatcagaag gacttcacaa tgaacaaagt aaaactttgg aaataataat atcagaaaat
    10741 attgatgtct taagtaaaga aacttatcta ggcaataaag aaattgagga actgaaaaat
    10801 ctaatactta ctttagggaa aagcgatata gagtcacaac aaagaatgat agatttatct
    10861 atagaaaatt tgaaaaaaca aacctatgag acaaaggaag atataaacaa aaaaggcgta
    10921 ttgtataaaa aattagtgac attcataggt ataggtatat gtattatatt gatttaatat
    10981 ggggaggttt attatggaaa tatcattgat attaaaggtt gcaggtgtcg gtattctaat
    11041 atcagtattg aatatgatat tggaaaaaac agatagaaaa gattgggcag gtcttactac
    11101 attagcaggt gtaataatcg tattgggtat ggtaataaca gaaataagtg acttgtttaa
    11161 tactgtaaga acaatgtttc aactttacta aagaggaggg agtaatttgg aaataatgca
    11221 attaatagga attgcaatca tttcaactac actctgttta gtaattaaaa aagatagacc
    11281 agagatagca aatttcatag ctataataac tggagtaata atactattgt cagttatgtt
    11341 taagctaaat tttatagtag atagtataca agatttggct aacaaggcta acatcccaac
    11401 tatgtatata tcattaataa taaaattaat tgggatagca tacttaatgg aatttgcgat
    11461 acaactttgt aaagattgtg gggaaggaaa cattgcttca aaactagaat ttggaggtaa
    11521 aataatagtt atgtcaatgt ctttccctat acttttgtcc attgtagaaa tggtggtgaa
    11581 tataattcca tagatgtgta tcttggaatg aatataagta tattagagag ctttcgttgt
    11641 attgaggttt atagagtgaa gagaggcatg gttgcttatg aaaaaaagat ttttaccaat
    11701 gattataggt tttttattta ctttcttttt tgtaaatatc tttgctatta tgatatttgc
    11761 taatgaagta ccaagtagtg aagataaaga atatggtgaa acaaaaaata gcatagataa
    11821 gtatatagat ggtcaattag acaagctaga tataaatgag attcaagact atataaataa
    11881 agaaatagtt attaatgatg ttaatttaaa atcatttgta aaagatttaa ttagtggaga
    11941 aaaaaatatc ttagatttat tcaataaaga tggattgaag atattgatgt ttgatgaatt
    12001 taaagcaagt ttaaaggtag tagcagtaat tttggtactg gctctgttat catctatttt
    12061 aaagagtctg gaaaactctt tttcatcagg tgcagtaagt caaatagcaa catatataat
    12121 atttatcaca atggtatcac ttacacttat tggttttaaa gatgtattac agatttgcta
    12181 tgatgcaata gaccatactg taggtcttat gcaagttata atgccaatat taataacatt
    12241 tttactttta attggattcc caataacgtc gactactttg aatcctatct tcataggagg
    12301 agtaacattt ataaatgtat tttttaaaaa ttttctattt gtatctatta ctgttgcatt
    12361 tggaatttta ataataaaca atttatcaaa aaatataagg ctaaagagat ttttctcctt
    12421 tgtaaaacaa ataaattatg tttctattgg agctatgttt actgtttact taggtcttgt
    12481 atccatacaa ggattgtatg taaccagttt tgataaattc agtgtaaaga ctgctaaatt
    12541 tgctataggt aattttattc ctgttgtagg tggatttgta tctgactctg tggatatttt
    12601 attatcttca tctcagctta taaaaaatat atttggagga ataggactta tcttacttgt
    12661 tggaatttgc ttacttccag taataaaaat tttatcagta atagtagtgt acaaattagc
    12721 agcaataatt gtagagcctg ttggagagga tggaatatct aattttttaa atgaagtggc
    12781 aaatttaatg ataataatgc tagcatcagt aattgctata acagtaatgt tctttgtaac
    12841 tgtagctatc ttgacctcga taagtgtcgt aagtcaaggg taatttatat atcagtgaaa
    12901 aataattaca tttaatctaa tgtttttaga gaggaggatg ctatgttaga gggcataaag
    12961 gcatggatag ttagtgtttt gataggagca tttatagtaa atatagtaga tatgatttta
    13021 ccaagttcta agataaaacc ttatgtcaac ctagttttaa actttatgtt tgtatttatt
    13081 gtaataactc cagtagtagg atttttttca aaagatatga gtttagaaga tagaatatta
    13141 aaatctatgg gaaactacaa caagcagtat gtagatagta caaatgcttt ggcaaaagag
    13201 actggaaata atagtctatc caagggatat gaggatgggt taaaagaagt acttaaatta
    13261 aaacttgatg aatatggtta tgatttagaa gatatagagc ttaatggggc aaatataaat
    13321 aatataaaaa taaaagaaaa gaataatagt gcaaaaagta ataacagttc caatgaagaa
    13381 aataaaaata atagtactaa aagagaggaa gaaaacataa actctaatga caaagaaaat
    13441 tctaaacaag tcttcaaaaa aggtacagag tatgggttga acctaaatga agaaaaatta
    13501 aaaaacgact taataaaggt gctggatgtt tctatagaag atatacaaat tgataaatag
    13561 gagttgggag aatgttgaaa aacttaaatg acaaagataa gagaaaggta tattcactaa
    13621 taactatagc aggaatatgt gtggtttcat tagtattatt atcttgtttt cctagtagca
    13681 aaagcgataa acaagttgga aaaacagaga ctaataaaaa tgctgaaaag caagtcacta
    13741 aagaacaaga aaaagatgat ttagagtcaa aattaacagc aatactatct aaaattgatg
    13801 gtgcaggaga cgttgatgta atggttacat ttgaatcaag tgaagaaata caacctgctt
    13861 ttaattcaaa taatacaaca gaaacaacag aggaaaaaga tgcacaaggt ggagaaagaa
    13921 cagttacgac ttctagtgaa aataaaacaa tgataacttc aaattcaagt gaccctgtag
    13981 ttataaaaac tacagagcca aagataaagg gagtgatagt ggtagcaagt ggagcaagtg
    14041 acccaagtgt taaagagaca ctttatagtg cagtacaaac atcattacaa gtagctggtc
    14101 atcaagtaga aatatattct aaataaatac atataaccgg ggggataaat gaatgaagtt
    14161 taattataag ggaagaggat ttgtaataat aactttaact gcaatgttag tggtagtagg
    14221 aacagtgaat tatcaactaa gtaagaaatc tttattagaa acatcaaaag agtttaaggc
    14281 atatgaagaa gcacagttgc aaaaaaatac tgatgagagt gatagtagta agacagagga
    14341 ttctagtaat gtagataaac aagatggtaa ggaaagtgct gatattgata tagttgatag
    14401 caaagctagt aaagtaaaag aaaaggcaac tgagacaagt aaggaaataa aagctcaatt
    14461 atcatcagag aaaaatatga aaaaggcatc ttatatttta gatatgaaaa tgaatagaga
    14521 aaaacaaaga aatgagttag ttcaagattt aaatgaaatg ataaataatc catctacaac
    14581 agaagaatct agaaaagaag cttctaatat gaaattaaat atagttaaaa ttcaagaaaa
    14641 agaacttcaa atagaaaacc ttttgagtac aaaagggtat gaagaagcgt tagtttatat
    14701 aagcgataac aaagttaatg tagttgtaaa tgaagctaaa ttagaaaaga aagatgcagc
    14761 aaaaatattt gatttagttg cagaacaagc caatgtgaag tatgaaaata ttaaacttac
    14821 aaataataat agtaataagt aaagtataaa cctcagaatt aactgaggtt attttcttgc
    14881 aaagtagact tgtatttggt attattatac ttataaaaat agttccttaa aatcaggagg
    14941 taagttttaa tatggaagat aataaatttg gacaagtaaa aatatctaat gatgtaatag
    15001 ctacaatagc tggtttagca gcattagaag ttgaaggcat agaaactaca gcaacattaa
    15061 cagataagtt gttaaaaaat aatggagtaa aaatacaaat cgaagaagag gatgttaatt
    15121 tagatgtaat ggttacgata aagtatggaa tgtcaatacc agatacagct tttaaagttc
    15181 aagaaaatgt aaaaaatact gttgagacaa tgacaggact gaaggtttct caagtaaata
    15241 tacacattca aggaatcagc tttaagaagg ataaagttga taaagaagaa gcaaaagcaa
    15301 ctaagaaaaa ttaatattag ccctctgaaa agtcagaggg ttttttgctg gtaattgatt
    15361 ttatatggat aattaaactt aaaggagaat tttgattgat gaaaaaagat agggcacaaa
    15421 agagtacaac aagagaatat ataatgaaac ttatatacca aataaatatt aataaagaag
    15481 attttgaaac tttagaagat aaagtagata attttttaaa agataattca gaacatatta
    15541 taaatagata taaagaactt gcacttcaat actcaaaaaa tacaaattta aagttagaag
    15601 atactgaaat tgaagatgtt atagacaaaa aatatataaa tacagtatgc aaagcattga
    15661 aagaaaatca tgacaaaata gatgagttaa taaataagca tgctaaaaac tggacagtag
    15721 atagaatgcc aaaggtagat gtttctatac ttagattgtc tgtgtgtgaa atactttatt
    15781 tagacactcc taataaagta tcaataaatg aagcagtaga acttgcaaaa atatattgtg
    15841 atgataagtc tcctaaattt ataaatggta tattgggaag tgttgttgat gaaattggga
    15901 aataatataa taattggaat tgatactagc tgctatacta cttctatagc agctatctct
    15961 ttagataaga aagttatatt caatgaaaaa ataatgttag aagtaaggga taattcaaaa
    16021 ggtttaaggc aaagtgaagc tgtatttcaa catatcaata atttagggat tcttagtgat
    16081 aggataaaat cttttaaaga taagtttaat gtagaaggag tttgctcatc aaaaaaaccg
    16141 agacctgttg aaaactcata tatgcctgta ttcaatgtag gtcataattt tggaaaatta
    16201 ttgtctagta tatatggttg taggttttat gagactactc atcaagagaa tcatattgaa
    16261 gcaagtttat taaatagtaa attaaaaaat aataataaat ttatttctgt tcatatgtct
    16321 ggtggtacta cagagatact tttaacaagc aaacaagata gtcatcacaa tgtatgtgat
    16381 actaatttag gtaaaattgc caaaataagt attaaaaaag atgataaaag taagttatac
    16441 aataattttg gatataatat agatataatt ggaggaagta aagatattag ctttggtcaa
    16501 cttatagata gagtaggaat taagcttgga tataaatttc cttcaggaaa gtatttggat
    16561 gaaaatgctt taaattgtaa tcttaaaata gaaagtggtc tgaaaacttc tgtaagagat
    16621 gggtatatga atttatcagg attagaaaat caggtaaata agattataaa tgataatgga
    16681 gataatacta atcaaaaaga atatatatct aaattggtat tagattcagt agttagaaat
    16741 atgtttaaat ctttagtgta tttatgtgaa acttataatg taaatgaagt agtatttgct
    16801 ggtggagttt ctgcaagtaa gtatattctt agagaattaa gcatgaagtt aagaaaaaag
    16861 catatagaag cttattttac agaaccacaa tattcaactg ataatgctgt cgggtgtgct
    16921 ataataggat tgaataactt tttaggagaa agagtatgaa actaagagct ttagatataa
    16981 gtgaagcaaa ctcatatatt aaaagaattt taataaacga cccaatactt tctaatctta
    17041 aagttaaagg agaaatttct aattttaagg ttcatagtag tgggaatgta tatttatcac
    17101 ttaaagatga aacatcaaag ttaaactgtg taatttttaa aagtaatttt aatcgcaatt
    17161 taaaattaga taatggtgta aaaattatag ctaatggata tatatcagtc tatgaaagag
    17221 atggtgcgta tcaattatac attaatgaaa ttgaaataga gggaatagga aatttacaca
    17281 tagagtttaa tagattaaag gaaaaactta ataaagaagg attatttgac cctaaataca
    17341 agatacctat acctaaaatg ccaaactcaa ttggcgttat aacatctcca acaggtgctg
    17401 ttataagaga tataataaat gtaataaaaa gaagatatcc gaaagttaat ataaagcttt
    17461 atccagtaac ggttcaagga gataaatcag ctgaagaaat ctgtgaagca attagatttt
    17521 tcaatcatat gaagaatgtt gatacactta tagttggtag aggtgggggt tcgatagaag
    17581 aactttggtc ttttaatgat gaaatggtag caagagaggt ttttaattct caaattccga
    17641 taatatcagc agttgggcat gaaacggatt ttactatttg tgactttgtg tctgatatga
    17701 gagccccaac gccatcagca gcagctgaga ttgctactcc atcattggat gatataaact
    17761 ataaacttgg aaacataaaa agtaggatga gtaagtctct tacaaatcaa atagagctag
    17821 accaatatag gttagaaaca gtatttaata agataaataa ctatttagat tcgtacacaa
    17881 taaaagataa agttatacaa ttagacaaaa tatatgataa aataattttt ggaatagaaa
    17941 ataatttaaa attagaagat gaaaaattag taaaaattgg agcattactc cataatttaa
    18001 gtccattagc tactatggat agaggttata gtataactca aaaaaatgga aaagtaataa
    18061 atagtataaa aggattaaaa ataaaggata gtatagatat agtattaaaa gatggaaatt
    18121 tagaatgtat gatagataaa attgaaaata aagagggata gtatgaattt aacctacgaa
    18181 gaagcttata aaagattaga aagtatttta agtgaattag aatctaaaaa tgctagcttg
    18241 gatgaatctt taagcctata tgaagaaggt ataagccttt ataaacattg taataagctt
    18301 ttagatgatg ctaaattgaa aataagtaaa tttaatcaat tgggaataga agaagacttt
    18361 aaaatagagg aggaataaaa ttggaattta aacaatgtct aaaagaaaaa gcaagttttg
    18421 tagaaaaagt acttaaagaa tatatgccta aagaagaagg gtatcaaaaa actgttattg
    18481 aagctatgaa ctatagctta agtgctggag gtaaaagatt aagaccaata cttacattgg
    18541 aggcttgtaa aatagttgga ggtaatgaag atgaagctat tccttttgct attgcaattg
    18601 aaatgataca tacatattca ctaatacatg atgatttgcc agctcttgac aatgatgatt
    18661 taagaagggg aagaccaaca aatcataaag tatatggaga agctatgggg attttagcag
    18721 gagatgcttt gttaaactat gcttttgaag taatgcttgc aggctcaatc aataaagaaa
    18781 atccagaaaa gtatctaaaa gctataaatg aaattgccaa aggtgctggt atatatggaa
    18841 tgataggtgg tcaagtagta gatgtagaaa gcgaaaataa acaaattgga aaagaaaaat
    18901 tagattatat acatatgaat aaaactgctg caatgatggt aggctgtatg agagcaggag
    18961 ctactatagg tggagcaaat tctgaacaaa tggaagaaat aactaaatat gcaaaaaata
    19021 tcggattatc attccagata gtagatgata tactagacat agtaggagat gaagctaagt
    19081 taggtaaaaa ggttggaagt gatatagaaa atcataagtc aacataccca tctcttttag
    19141 gtttagataa atctaaagaa attgcacata atttgattga tgaagcaaag aaaagtatag
    19201 aaaaattatc tgatgatgta gattttttaa agggattggc tgaatatata atagatagag
    19261 aatactaaaa tatacagcta aaatcgagga gggaaaaatg gatttttttt cggagatttt
    19321 taacaatggg gctttgggaa taagtctgat tgcttgtttc ttagcacaat ttataaaaat
    19381 atttactggt aaggaaaaaa gaattgagtt atcaagaata cttatttcag gaggtatgcc
    19441 tagttcccat agttcttttg ttacaagttt agctacagtg gtaggtattg aaaaagggtt
    19501 taattctact gattttgcta taattaccgt gcttgcctta attataatgt atgatgcagc
    19561 aggtgttaga cgggctgttg gtaaacaagc tactatatta aatcaaatgg tggcagatat
    19621 acagcatgga aagcatatag agcagaagaa attaaaagaa ttaataggtc atacaccact
    19681 agaagtttgg tttggagctt tacttggaat agtaacagct ttaatattga tgtaaattga
    19741 aaatttacag aataaaggtg aggataatat gtataaatat ttagataaag taaattctcc
    19801 aaaggatata aaaaatatga gtatagaaga aatggattta cttgcaaagg atataagaaa
    19861 gttcttagta aaatctgttt caaagacagg aggacactta gcttctaatc taggagtggt
    19921 tgagttaaca ttagcacttc ataaagtatt tgatagccct aaagataaga ttgtatggga
    19981 tgtagggcat caatcttatg ttcataaaat agttactgga agaaaagatt gttttgtatc
    20041 tttgagacag tttaatggac taagtggttt tccaaaggag aatgaaagtc ctcatgatat
    20101 ttttgataca ggacatagca gtacatctat ttctatagct acaggtattg cctgtgctag
    20161 ggatataaaa aaagaaaact atagtgttat atctgtgata ggagatggtt ctataacagg
    20221 aggtatggca cttgaagctt taaatcaatt aggatatata gataccaata tgatagtaat
    20281 ccttaatgat aatgagatgt ccatagataa aaatgttgga ggaatgtcta aatatctgtc
    20341 aagtattata agaaactcaa cagttgagaa aatgacagat gaagttgata aaattttaaa
    20401 tgttacacaa acaggtgaaa ttttatctaa gactgcacat agatttaaag ataaactaat
    20461 gtatagcttt tctcctcagg actgttcatt ttttgattca ttaggtataa gatattatgg
    20521 acctatagat ggccataata ctaaagaact aatagatata ttgagaaaag ctaaacacaa
    20581 aaaagggcct gttcttttac atgtaattac gaaaaaagga aaagggtaca gatttgcaga
    20641 agaacaacct gataaatatc atggagtatc aaagtttgat ataaagacag gtgttacatc
    20701 tgcaaaagtt aagtctatgt caattagtgt tggagaaaaa cttgttgata tggctaatag
    20761 taatgaaaat atagtagcaa taacagctgc aatgccatct ggtacagggt taaacctgtt
    20821 tgaaagtgca tatccaaaaa gatattatga tgttggtata gctgaacaac atgcaacagg
    20881 atttgcagca ggtcttgcaa aaaatggtat gaaaccttac tttgctgtgt actcatcatt
    20941 tttgcaaagg gcatatgacc aagttattca tgatgtatgc ataacaaaaa agccagttac
    21001 ttttcttata gatagagctg gattagttgg aaatgatgga gaaactcatc atggtatgtt
    21061 tgatttaagt tacttaaatt ctattccaaa tattgtagta atggctccaa aagacacaag
    21121 agagatggag cttatgatgg atttgtcatt aaaattagat tgtccactag caattagata
    21181 cccaagagga agtagttatt acctagataa gggagagtat ggagagattg tactgggtaa
    21241 atatgaggta ttagatgacg gtcaagatac agtcatactt tgtattggaa gtatggtaaa
    21301 acatgcttta gaggctaaag aaatattatc aagagaagga ataaatccga caatagttaa
    21361 tgctagattc ttaaagccaa ttgatgaagg tatgttaaaa gcattattaa aaaatcataa
    21421 aaatgtagtt actatagagg ataatattgt aactggagga tttggaagta gaataaataa
    21481 gtttattata gataatgaat ataacgtaaa tatacttaat atagcaatac cagaagaatt
    21541 tgtaaaacat ggcaatatag atgaattata tgattttgta ggattatcgc caaaaagtat
    21601 tgctgataaa ataaggaaat tagttattga gtaaaaatct aatgtagata ttgtaaatta
    21661 aaatttatat aaaatataga atttatcaat tagaataaat atcaagtgtg ttacttcaag
    21721 ctactttagt tatcattaaa gtagctttta acagaaaggt aattgtatga aaaaaagaat
    21781 agatttatta ttagtagaac aagggtactt tgaaagtaga gaaagagcca aaaaagcaat
    21841 aatggctgga ttggtttttg ttgataatca aaggtgtgat aaggctggta cagaagtaaa
    21901 agaagattgt agtatagaag taaaaggaaa tccaatacca tatgtcagta gaggtggttt
    21961 aaagcttgaa aaagcaatga aaaactttga tttgactatt gatggaaagg tatgtatgga
    22021 tataggggca tccacaggtg gttttacaga ttgtatgctc aaaaatggag caataaaagt
    22081 attttctata gatgttggtt atggtcaact tgcatggaaa ttgagacaag atgatagggt
    22141 tgtgtgtatg gaaagaacca atataagaaa tgtgacaata gaagatacaa agcagtttgc
    22201 tgattttgcg tcaattgatg tgtcttttat atcgttaaag ttagtacttc caaaggcaaa
    22261 ggagttagta cgtcatgatg gggaagttgt cgctttaatt aaacctcaat ttgaagcagg
    22321 aagagaaaaa gtaggtaaaa aaggagtagt tagagaaaaa tctacacata tagaagttat
    22381 aaaaatgatt tctgatttct cagtcgaaaa tggatttgag attttagggt tagatttttc
    22441 tcctattaaa ggtcctgaag gtaatataga atatctaatt catttgagaa atggaaatga
    22501 aggatatgaa tttgatggtg agacatacaa taacaaaata gtagaagttg ttgaagcttc
    22561 tcataattta gacaaatagg ttaaaagggg gcttagattt gatcctcgaa ttatatatga
    22621 aaaattgtgc cttagttgaa gaactaaggt tgaatattga taaaaactta aatatactta
    22681 caggtgaaac tggttcagga aaatctataa taatagatgc acttggactt tgtttaggtg
    22741 ataaatatga taggtctttt ttaagaaaag gaacagataa gggacttgtt gaggctgtat
    22801 ttttttcaga caatagatat cttaaaaaaa tattagaaga aaatgatata agtatggaag
    22861 atgataattt attggtaata actaggttga tatactcaga tggtaagagt actgctagag
    22921 taaatggaag gactgttaaa gtgtctcttc ttaaagaaat tgcatctaca cttatagata
    22981 tacatggtca acatcaaaat caagctttgt ttaataaaga tactcattta aaatttctag
    23041 acctttttgg agaaaatgaa ttggaagagt ttaaaattgc ttataaaaat gtgtaccata
    23101 agtatagtga agtaaaaaaa gctttaaatt gcttgacaga aaataaagat gagatgcaaa
    23161 ttcaacgaga aatagattta ttaagatttc aaataaatga aattgaagct gcaaatctaa
    23221 ataagaatga atatgaagat ttattaaaac aaagagaagt ttatagaaat agtgaaaaga
    23281 tatataataa tctaaattcg agttatagca agttacataa tggtgagtat aatgttattg
    23341 acctaatagg tcttgcatct aaggaactta atgacatatc taaatatgac agtgttttaa
    23401 gtgaatatag tgatactgta gaaagaatca tgtatgagtt acaagacata tctggtgaga
    23461 taagaaatta caaagataat atagactttg aaccttatga gctagagcag attgagctta
    23521 gaatcgacga gattaataac ttaagaagaa aatatggaga cagtatagat gatatatttg
    23581 agtattatgg aaaaattaaa gatagattag atgaaatatt aaacagagat gaaagagttg
    23641 aacagttaag aagcaaactg atgaatatag aagaagattt aaaaataaaa gccagtaaat
    23701 tgactaaagc aagaatagaa gtagcaacta ctttagaaaa aatacttctt gatgaattaa
    23761 aaagcttaaa tatgaaaaat gtaatgttca aggttaattt tgaaaaaggt ccatttactt
    23821 tagatggagt agatgatatt gaatttatga tatcttttaa cttaggagaa gatattaagc
    23881 ctatatataa agttgcatca ggtggtgaga tgtccagatt tatgttagcg tttaaaacca
    23941 tattggctga tatagatgat attgatacat tagtgtttga cgagatagat actggaataa
    24001 gtgggatagc agcacagata gttggtgaga aattgagtga tatagcaaag aaaaaacaga
    24061 taatatgtat aactcattta cctcaaattg ctgcaaatgc agacactcat tattgtatag
    24121 agaaagatac ttcaaataat aggacgttta ccaatgttag taagttaaat gaatctcaac
    24181 gaaaaaatga aattgccaga cttatagcag gtaacaatat aacagaaaaa actattgaac
    24241 atgcaagtga aattattgag ttggctaaaa aatgttagtg aaaaacaagc attttaatag
    24301 gtgtaaagaa tttttataga aatgctttac atctattttt tatgatgaaa ttattttaaa
    24361 atatctagtg cctatattga agaaaagtag atttaaaaat agaagaaaat ttgtataaat
    24421 ttgtaacttt gaaatttcta aatgcttaag tataatatta tttattaagg tagtagtgag
    24481 ggggacataa aatgaataaa aaagatacta tgcacttaat ttttacaatt gtaattttta
    24541 ttttattatg gtatattgtg gtagcagtaa gtgagattaa taaaaatatt gaaagttgta
    24601 gtaataaatt agattatttg tcacaagaga ttatgagtac taaatcggat attagtaata
    24661 ctataaatga agaaatgtca aaaagttata taacaaagag tattgactta aaagtaaaaa
    24721 aaatcgagaa aaaagagtgt gttattgatg tagatgttca attaagtaaa cttggaaaaa
    24781 atggaaaagt attttttatg tataaagaag atagtgataa atggaatgaa actgagatga
    24841 caaaacatgg agaattatca tatgcatgtg aaattaagat aaatacagga agtgaatatg
    24901 actataaagt agttacaagt ggagctatat ctgaaagttc tgatgtgcaa aaattgacta
    24961 aatctgatta tatgcctgaa caacccatat ttaattctgg gataagagat aatagagaat
    25021 actttattga aatagtagaa aatagtaatc ttttcaatca tgagagtgaa aagtcaaaaa
    25081 ataaagttta tgataatatt agattagaaa aagtagatat tattgtaaat gaaggaaaaa
    25141 aagacactat atacaaagcc aaattagctg aaggcttaga tgatggcaaa actaataatt
    25201 caaatagaaa tcaagtgaat tatgaagtaa gttttcctaa aagggatatt gatgacataa
    25261 tttatattaa agctaaactt acatacaata atggagtaga atatacaaag gatataactg
    25321 aagatataac tatgggatta caagatttag aaaaataaat acatatttaa ataaatattc
    25381 aaatagattt gttcaataaa atagagtata aataaaactg tagtagtttt acttatactc
    25441 tattaaaaaa ttataatttt aattccatat atctagcccc ttcaattgga ttataagtat
    25501 aaggttctat atcataaaaa ccaaactttt catataatcc ttgtgcactt ttcatgcttg
    25561 gaagagtatc taatctcatg tgtgtatatc caaatttttt agcttcttca atgatttctt
    25621 ccaatagtat ttttcctatt tttaatcctc taaattgatt tcttacatat aatcttttta
    25681 attcacatat tttaccttca agttttttta aagcaacaca acctgccaaa ttttcatcaa
    25741 caaatgccaa tatcaatgag cctgaaggtt ttttatattt tcctggaagt gtttttaatt
    25801 cattattaaa gtcttgaaaa cataagtcta tatttagaga atttgaatat tcagtaaata
    25861 gaagttttac gttttcaagg ttttctaaat ctttttcttc aacaaattgt aaagttatat
    25921 tttgagccat tttatccgcc attatataaa catgtttata taatgacttc acctccttga
    25981 tatatgtata tttttcttaa tatcaattag attttaatac cattactttt tcaccaccat
    26041 agtcaagctc tccattaaac tcaaatccaa aatctttata aagcttaata gccattttat
    26101 tgtcttcgaa tatactaaga tatagttcat cacagttata ttcattttta agatgattta
    26161 caagaaaatc taatgatgct tttccatatc ctttaccttg atattttgaa tcaatcataa
    26221 atctatcaag ccaaactcta tcatcttcta aacgaccata catagcaaat cctattaaag
    26281 aattttcatc atatattcca acaggtaccc aagctgttat aaatttagat tcagcaatag
    26341 aaagtgcatt gctttctata taatctaact gacttttatc tacagacaaa ctaactaaat
    26401 catgccaatt atttatgtct gcaattctta catttaattt caagatatta ccgcccttca
    26461 aactttttgt tacaatctaa tactatcata ttatgtatta aaaatcactt attaagattt
    26521 taaataaact ttttaaatga ataaattctc aggaaaaagg aattataaat acataataat
    26581 acagcatagg agtagatgga tatatgaaca aaataaataa aaatttcaaa aatatttgga
    26641 taatattagg agctgtatcg atttttacca tggttataat ttttaataaa aattgtaatg
    26701 ttaataaaga gactcaaata gaagctttta caattaagaa tcatgataaa aaatttgtat
    26761 atcctatggg aaatataatt ggagtaaaag caaatacaga tggtgtattg gtcttaggat
    26821 atgaggaaga ggatgtagac tatattggag gcatacaaat cggtgataat atagtaaaaa
    26881 tagataataa aaggataaaa aatagtcaag atgtatcaga gattttgaat aagattaaaa
    26941 aaagtaaagt tgaagtgact tttgagagaa aaaataagta taaaacagaa gtaatagaga
    27001 ctaaaaaaga aaatggcaag tataaacttg gattatgggt aagagataaa atatctggaa
    27061 ttggaactat gactttttac gaccctagta tggaaaagtt caaagcaatc ggtcatgcca
    27121 taaaagattc ggatactaat gaactattaa agataaagca aggatatata tataagccag
    27181 aacaattaaa aatagtaaaa gctagtaatg aaaaagtagg taaaataaaa ggagatttta
    27241 atgacagcaa tttaatgggt aatttctcaa ataattcaga gttaggtata agtggtaata
    27301 ttacagaaaa ccataataaa gagtttaatg tagcaaataa agaaaagcaa ttaatagaag
    27361 ttggaagacc gcaagatgta aaaataggag atgcagtaat tctttttgag gataaaaaca
    27421 aaaatataac aagctatgat ataaaaatag aaagtatagt atatgataaa ggaaattata
    27481 gagatatggt aataaaagta gtagatgaca agttattgga atacacagga ggtatcgtac
    27541 aggggatgag tggagctcca ataatacaaa ataataaaat tattggtgca ataactcatg
    27601 tttttagaga taatccgaaa aaaggatatg gtatttttat agatgaaatg ataaaattgt
    27661 aggtgaggca ttaaaaaatt ttattatttt atcaattatc taggaggaat ataattttgg
    27721 agtgtcgaat atgctttaga gtagataatt aggaagcaat tgtgtaaaaa gtttagtttt
    27781 ctgtaataag aagatgtttt ttaatggggg gatttttagt ggaaaaaatc aaaatagttt
    27841 tagcagatga caataaggat ttttgtcagg tattaaaaga gtatttgtct aatgaagatg
    27901 atatcgatat attaggcata gctaaggatg gaattgaagc attagactta gtaaaaaaga
    27961 cacaaccgga cttattaata ctagatgtaa taatgccaca tctagatgga ttaggtgtaa
    28021 ttgaaaaatt gaatactatg gatataccta aaatgccaaa aataatagta ctatcagcag
    28081 taggtcaaga taagataact caaagcgcaa taaatctagg agcagattac tacatagtaa
    28141 agccatttga ttttgttgtg tttataaaca gaattagaga gctagtttct aatagagtta
    28201 cacaagttga gccaaaacct agaccagttc aagagactca aatgactaga agtgattttg
    28261 ttaagaatgt aggaaatata gagacagaaa tcacaaatat aatacatgaa ataggagtac
    28321 cagctcatat aaaaggatat ttatatttaa gagaagcaat aaaaatggtt attgataatg
    28381 ttgagctttt aggtgcagta acaaaagagt tatatccaag tatagctaaa aaattcaata
    28441 caacaccaag tagagttgaa agagcaataa gacatgcaat agaggttgca tggagtagag
    28501 gaaaagttga cacaataaat caattatttg gatatacggt acacaatact aaaggaaaac
    28561 caactaattc agaatttata gcaatgattg ctgataaatt aagattagaa catagtatgg
    28621 ttaaataaac aagacataaa aagtaaggct ttttaattaa ggcattggct ataaatgcgt
    28681 attacaagca gcgaaacggt ataaccacta gggttatacc gtttcgctat tttaataaat
    28741 ata
//
LOCUS       DAELTA010000035        28272 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.035, whole genome shotgun sequence.
ACCESSION   DAELTA010000035 DAELTA010000000
VERSION     DAELTA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.035"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..888
                     /locus_tag="KPG13_001321"
     CDS             <1..888
                     /locus_tag="KPG13_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985058.1"
                     /translation="VLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEE
                     ETILSRINHRLIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLL
                     NQTFKTEIQKDFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATAL
                     SGILFDAEKKNIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATEC
                     WVMYTKIDIIDNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYI
                     KVTSDIGKGSTFSIFLPNK"
     gene            970..1635
                     /locus_tag="KPG13_001322"
     CDS             970..1635
                     /locus_tag="KPG13_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985059.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            1648..4065
                     /locus_tag="KPG13_001323"
     CDS             1648..4065
                     /locus_tag="KPG13_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985060.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(4246..5037)
                     /locus_tag="KPG13_001324"
     CDS             complement(4246..5037)
                     /locus_tag="KPG13_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0985061.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5070..5657)
                     /locus_tag="KPG13_001325"
     CDS             complement(5070..5657)
                     /locus_tag="KPG13_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985062.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(5834..7225)
                     /locus_tag="KPG13_001326"
     CDS             complement(5834..7225)
                     /locus_tag="KPG13_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG0985063.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(7409..8713)
                     /locus_tag="KPG13_001327"
     CDS             complement(7409..8713)
                     /locus_tag="KPG13_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG0985064.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(9072..9947)
                     /locus_tag="KPG13_001328"
     CDS             complement(9072..9947)
                     /locus_tag="KPG13_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG0985065.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(10151..10837)
                     /locus_tag="KPG13_001329"
     CDS             complement(10151..10837)
                     /locus_tag="KPG13_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG0985066.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(10886..11419)
                     /locus_tag="KPG13_001330"
     CDS             complement(10886..11419)
                     /locus_tag="KPG13_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0985067.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(11463..12095)
                     /locus_tag="KPG13_001331"
     CDS             complement(11463..12095)
                     /locus_tag="KPG13_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG0985068.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(12185..13264)
                     /locus_tag="KPG13_001332"
     CDS             complement(12185..13264)
                     /locus_tag="KPG13_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0985069.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(13397..14743)
                     /gene="pepV"
                     /locus_tag="KPG13_001333"
     CDS             complement(13397..14743)
                     /gene="pepV"
                     /locus_tag="KPG13_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG0985070.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(14812..16092)
                     /locus_tag="KPG13_001334"
     CDS             complement(14812..16092)
                     /locus_tag="KPG13_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG0985071.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(16219..16479)
                     /gene="hfq"
                     /locus_tag="KPG13_001335"
     CDS             complement(16219..16479)
                     /gene="hfq"
                     /locus_tag="KPG13_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG0985072.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(16593..17528)
                     /gene="miaA"
                     /locus_tag="KPG13_001336"
     CDS             complement(16593..17528)
                     /gene="miaA"
                     /locus_tag="KPG13_001336"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG0985073.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(17566..19533)
                     /gene="mutL"
                     /locus_tag="KPG13_001337"
     CDS             complement(17566..19533)
                     /gene="mutL"
                     /locus_tag="KPG13_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG0985074.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(19544..22387)
                     /gene="mutS"
                     /locus_tag="KPG13_001338"
     CDS             complement(19544..22387)
                     /gene="mutS"
                     /locus_tag="KPG13_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG0985075.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(22547..23281)
                     /locus_tag="KPG13_001339"
     CDS             complement(22547..23281)
                     /locus_tag="KPG13_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985076.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(23250..24263)
                     /locus_tag="KPG13_001340"
     CDS             complement(23250..24263)
                     /locus_tag="KPG13_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0985077.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(24285..25055)
                     /locus_tag="KPG13_001341"
     CDS             complement(24285..25055)
                     /locus_tag="KPG13_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985078.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(25030..25320)
                     /locus_tag="KPG13_001342"
     CDS             complement(25030..25320)
                     /locus_tag="KPG13_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG0985079.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(25451..25550)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      26170..26259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            26312..26488
                     /locus_tag="KPG13_001343"
     CDS             26312..26488
                     /locus_tag="KPG13_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985080.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(26603..26959)
                     /locus_tag="KPG13_001344"
     CDS             complement(26603..26959)
                     /locus_tag="KPG13_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985081.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            27426..28174
                     /locus_tag="KPG13_001345"
                     /pseudo
     CDS             27426..28174
                     /locus_tag="KPG13_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
BASE COUNT         9498 a         4589 c         3160 g        11025 t
ORIGIN      
        1 gtactttgtg gatttatagc attaataata tttttacttc ataaaaaaat agttatattt
       61 tgttctagaa tatgtcacac tttagatagt atgatgaata gtgaagataa tattgaaatt
      121 gaattagaag aagaaactat tttatcacgt atcaatcaca gattaattag aatgtatgaa
      181 gttattcaag aaaatcgcaa tagtattgct atagagaaag ctgatttaca agaactagtt
      241 tctgatatat ctcatcaaat taaaactcct attgcaaatt taaaaatgat taatactact
      301 ctactaaatc aaacatttaa aacagaaatt caaaaggatt ttttaatgga tatggaaaac
      361 caattagata aattagattt tttaatgcaa tctatggtaa aaacctccag actagagaca
      421 ggtataatca cactatctaa aaagaaaaat tcaatttatg aaacaattgc tacagcactt
      481 agtggaattt tgtttgatgc tgaaaaaaag aatattgaag ttacagttga ttgtgaccca
      541 aatttatata taattcatga caagaaatgg acttctgaag ctatttttaa tatattagat
      601 aatgctgtaa aatacacaag ttctaatggt aagatacgtg tagctacaga atgttgggtt
      661 atgtatacaa aaatagatat tattgataat ggaaaaggaa tatcagaaag tcgccaagca
      721 gaaatcttta aaagatttta tcgtgaagaa gatgtccatg acatagaagg tattggaata
      781 ggtctctatc tttcaagaaa aattatatct cttcaaggtg gctatattaa agttacatca
      841 gatattggaa aagggtcgac tttttctatt ttcttaccta ataaataaat ttttaaatat
      901 ttcatttgta atgattctgc aacattttgg ttttatactg tatttacaag atgttaagga
      961 ggtattatta tgagtatttt acaagtaatt aatttaaaga aatattatgg acaaagttca
     1021 aacattacaa aagctcttga tggtgtaact ctatctattg aaaaaggcga atttatttct
     1081 attgtaggta catctggtag tggcaaatcc acattattaa atatgatggg aggattagat
     1141 aatcctacat ctggaaaggt tattataaag ggtaaggaat tatctactat gtctgatgaa
     1201 cagcttacaa tctttagacg cagaaatata ggttttatat ttcaaaatta taatcttgtt
     1261 cctttactta atgtatatga aaatattatc ttaccaatag gattggatgg agagaccata
     1321 gataaggatt ttataaatga aattataaat atgctttctc ttgaagaaaa attatatgat
     1381 ataccaagta atctttctgg tggtcaacag caacgtgttg ctatagctag agcacttgta
     1441 acaaaaccag ctattatact agctgatgaa ccaacaggta accttgatag tagaactagt
     1501 tcagatgtac tgagtttact taaagtgaca agtaaaaaat tcaaccaaac aattgtaatg
     1561 attacacaca ataatgaaat tgcacaacta actgatagaa ttattcgtat tgaagatgga
     1621 aaaattgtta actaggaggt gtagtatatg tttagaaata ataatcgcac atttataaga
     1681 aaaatagctt taaatgattt aaaaattaat aaacttaaaa catatctttc aggtatcatt
     1741 ataatgattt ctacatgttt attattaaca gtgaccttag tttcatataa tgcctctgta
     1801 gatttagtta atgcatctcc atatcatgct atatataaat ctgtagatga aaaagcaaaa
     1861 aatatactat ataaagataa aaactttagt ggcgtaggaa cttacaaatt aattggaagt
     1921 aataaaaaga cagattgtat tatgtcgatt gtttatgcag atgatacagc aattaaactt
     1981 atgaattttc aaccattgaa aggcaaacta cctacaaata aaaatgaaat tgctatttca
     2041 gaaaaatatt tacaggaatt tggtttagat aaagatattg gagatagcat taaattaaat
     2101 tattatgatg atattacgaa taaagaagtt caatgtgatt ttattattgt tggatttcta
     2161 gaaaattatt atcaagataa tgccaagcaa tactatacag tcgtttcaaa tgattatttt
     2221 aagaatatag cttctacacc tttacaaaat aaaagtaata cttttgacga gtcaagacct
     2281 gatactgtag atgtattagt taaactatca gaagaaaata cagagaaaga ttcagcttca
     2341 attaagacac agttaaataa aattggttta tctttaggta ttaagaaata taatatatac
     2401 ttaaatgata actatattga atcaaattta attgatggtg agcagattat tacagtttta
     2461 gtagttggaa tagttgttct tttttctagc gtttttgtta tatatagtat tttttatatt
     2521 tcagttgtta attcagttca gatgtatgct aagttgaaat cgttaggtat gacttctttt
     2581 cagttaaaaa aaattatttc tctacaagga aatattttgt caattatttt tattccatta
     2641 ggggttatag cttcttgtat aattgcttac ataattcaac cattagcatg gcaaatgata
     2701 gctgatttat ttataatttt aattttatca ctggtaatgt ttttaactgt tagaatttct
     2761 ttatttaaac cagctagaat tatttctaaa atatctgcta ttgaagccat gcaatataca
     2821 gaaactaaat ttagaaaaaa agcaaaaaat tttagttata taaatataaa aaatctagca
     2881 ttaaagaata tcgagtcaaa tagaaagaaa aatttgattg cattaatatc gctaagcatt
     2941 agtggagttt tatttatttc aatcgctaat cttgctaatt caatagattt caaaaaacaa
     3001 ctatctcaac aatttgtcta taacgaagat tatataatta gcattaagag tgataactta
     3061 tatgagcata ttacagaaat ccaacaaaat aatccactaa cagattctct taagaatgaa
     3121 attaaatcta ttcatggagt aaaaaaatta attgaatcca aatcaattaa aagtaatatt
     3181 atagaacctt acatcaaaga tagagacgaa aaacaattta ttactctaat taaaggtatt
     3241 acgcctgaac tttcaaaatc acttgaagaa tatattgtca gtggaaaaat agactataca
     3301 catttagatt cagatagttt aattataaat aaatatagaa caaaatatta tggattaaat
     3361 ttaaaagttg gagatacagt tacatttaat atctttagtg gaaatgatat tattaaaaag
     3421 aaaatgaaaa ttataggaat cattgacaat tccaatacag gaggtatgtt ttttgcttca
     3481 gacaaaactt tagaatctct aacaccatat aatacaaatt tagatttttc tattttaata
     3541 gataaaaagc attctaaaga agttgaagaa aagttgttaa gtataattca gaaaaattca
     3601 aatttaatgc tttattcata tgaagatgat tatagaatga ttactagagc atttcaatat
     3661 ataattatag cttcatacat ttttgttggt ttaatttctt gctttggtat actaaatatg
     3721 accaataccc taataaacag cgttttaatt agaaaaaaag agtttgcatt acttcaagcc
     3781 gttggaatga caagaaagca actgcgaaat atgctttata gagaaggtct aaacataagt
     3841 atcaaagcta tatgtacttc aagtatacta ggttattttg gttcaaatct actttgcact
     3901 tttataaaag atgtaattag attagacttt attaatttta aattttctat atttactatt
     3961 ttaatttttt cattcgtatt aattggtata caagttttag tgactgaatt acttgtaaga
     4021 aatatagaaa aaaaatcact tacagaacgc ttacgttctc agtaagaatg tatataaaaa
     4081 atagccattt tttatacaag ttttaacata attttttaaa agctaaaagg atagaaaatt
     4141 ttgactttac ataattttct atccctatac ttgtttttat tttatacaga tacacttact
     4201 tatatctccg agcaacatca ccagagacat aaacaagcat attccttatt ttctaaatat
     4261 gtcaattgca tgacttgtaa cccctactaa atcttcacgt tcacaagtag caaggtgata
     4321 ttttaaatat aatgcaaatg catcatcatc catagagtct actgcttcac gcatatacaa
     4381 agccaaacca tctgatgcga cataatgcag tctagttaca ttaaatgcag acatcaaatc
     4441 atcaatgttt tctttacgta caagctcaaa caaatccttt ggttcagact tagcagcaaa
     4501 tgtctgtgga tcaatcaaac ctttttcaat atattcagat acattaatat ttcctctatg
     4561 gaacccctcg tcaataagac agccatcaga tataacatat gcagcaaaaa ccacaccacc
     4621 aggttttgtt acacgaattg cctcatgtaa agcttgttgt ttatcttctt tgttataaag
     4681 atgatatagt ggtcctaaca gtaacgtaat atcatattta ttatctggga aaacagataa
     4741 atccatagca ttaccctgag taatgcttat attttcattt gattgagtat ttttatggaa
     4801 aacctctata ttatgttcga ctaattccac tgcgtcaaca tcatatccct gacgtgccag
     4861 tgtgtgagaa taacgaccag ttgcagcgcc aatttcaagc acacaatcac ctgattttat
     4921 gtacttatca atgtaatgca tagtagtgag aaattccacc attccatgtt ttaatgcaag
     4981 tcgactatct tcatcataag tattataaaa gtcaattaag tattgttttg tttccataat
     5041 ttcacctctt ctatattagt taataaacat caaatagaca aattccaatc tgatttttct
     5101 tgtcttataa aagctgttaa tatatttttc aacatttttt cagcactata tctatctaat
     5161 gttaattttt ttgttgctgt taagacagct atgccatgag tataaagaaa taaatctaaa
     5221 aatattactt ttgcacattc caaatctatt ccaatgcttt ccgaaaaaag tcttgccttt
     5281 ttttcattgt caatttcttt ataaaaatca ttcgcctctt tcatcatcaa atctaaatca
     5341 tttataaata acaacttaaa taaatacgtt tcttcttgag caaattcaat gtatgaaagc
     5401 ggaagcagta aataagggct tacactttta gaattacgat aattaacaac atactgatta
     5461 taatattcat atgcaaaagc aagaaattca ttttttaatt cattcatgtt ttcataacaa
     5521 gtaaaaattg gtcgagtgga acactgtaat ttacttgcaa tactcctcgc atttacagct
     5581 tcaaatccca cttctcttgt aatatccaag actgtattca aaatcatttc ttttgttatc
     5641 tttactttag gtggcatata tgttcacctc tccattaaat ttttataaaa cacatgtgtt
     5701 atataactat agtaacatat caagaaatta tgtcaagaaa aagctttgca tttttttgca
     5761 attcctaaaa cttataaata aaacatgagt tgcaattaat ctattaatag ataacagcaa
     5821 ctcagatttt cctttaaata ctcttctttt tgtctaatat ttcatataca caatcttcta
     5881 ttgaattaat atctttttct tttctttttt tagaatcaat taaaatgaaa tacagtataa
     5941 aacctacagc aagtccccat gttccgcctt tagcagcaat tacacctccc ataagtccaa
     6001 tcaatccctt ctcaacacct gtattacata gctccattga tagctttgta cataaatatc
     6061 cttgaactaa aagtgttact gacaatgcta atggtataag aggtgtagca atctgcgcaa
     6121 ttggataaaa acagataaaa attgctgaaa cccatactaa tgtacccatg ccactaaaaa
     6181 cagactgcat ggcttccttt ccaaccttgt acctttgagc aacacttcct gtaagtgtcg
     6241 cagctaatgg accgcacatt ggtatatatg gagaaaagat tgccataaaa atatttctta
     6301 tcccacttat aacattagac ctatttgaat tgaaatctat atattcatct tttctatttt
     6361 cactttcaga aagtactaag ttttcacctg ttataaaatc accaaaggca attatgtata
     6421 tcataaaagc cattggtatc ccttgtataa ataacattac acttggaaaa cctattccaa
     6481 aaggagatac tgtatttatc agatttttaa aatctactat gtaaactaaa ttgtcaaaac
     6541 taaatctagg aatttctatt tctttactaa atatacctac tattaaactc actaaaatag
     6601 ctgatactac accatattct cctaaatgaa ataaaaattt attcttaact tttagagtat
     6661 caaatctttc agaaaacatt acaaaacaag caaccaaaat tcctactgtt atagatatag
     6721 gataagaatc aaatctccct ttaggattaa actctgatat tatagtcgat ataccgacac
     6781 ccattagtat tccaccttta atagaatttg gtatcttact aactaacttt ccagctacac
     6841 cagttatacc cattaaaata aatattattc ctaaatctat ttgtaaagct gttaatgctt
     6901 gtattctttc aatgcccatg ctgaacttta acaaatatgc agttgtaaga gttatagaag
     6961 ctgtaatcca gccaggtaca actgggtctc ccataaaaac atgcaaacag tataatattg
     7021 agttaatgat agccatactc catgccactt catagggtaa tcccaaaatt tgtgtactaa
     7081 ggccaattgt gcctaaactt gtcatacttg atattatacc ttgaatagct tctggtttct
     7141 caaatctgta atgtataaat ggaattctta tcttaaacat tccaaatttt atatgaggtt
     7201 gttcccttct agcgagtctt tccataactt cactccacct ttcaataact cttataagtt
     7261 tacaacttta taattgttta taaattatca atgtcattaa aattagtata taacatgcaa
     7321 attatataaa caataaatta aatctataaa attgatagat tttgtgcata tatagaaaga
     7381 ccgcttcatt tttagttact taaatcactt aatagtagtt atttaaatca cttgataaag
     7441 gttttttata aaattcaata gttctattcc atgattcaaa ctgtcctttt gaatttcctt
     7501 ctacagttcc acctgaagaa aaaatccctc cactcatact catacataaa gtctccttta
     7561 gaggcataat atatggaatt ggtaacggat gacccatatt attgtatgtt aataattcta
     7621 cgttatatgg ataattattt tcttcaaaga tttctaatgc ctttaaacaa gcttcataac
     7681 tattccatat gttatcttct tttccagcaa tcataagtat atctgctttg gaattttcta
     7741 tttttattct agcctctttt ctattctctg ctcttttaag acttttctta taagtactat
     7801 taaatccaaa tggaatacct tttgatatat tttttttctg ttcttcaaag aatatatcat
     7861 tgtctacttt gatatatgga aatggctctc ctttatatga ccatgatgaa gtatcatttc
     7921 cactcatcaa tccattaaga gcttggaaac aataaatgtg aggttgtgat actacaacct
     7981 ttttaatttg ttcatatctt gaagcaagta aaaaggccaa ttctccaccc tttgatgtcc
     8041 catgtacaaa tatttcttta gtactggtaa tttcattttc actaatccac tcaaatactt
     8101 tttcaaaata ttctaatgga acctcttcta atttatctgg taacccttct aaagcaaagt
     8161 atgcaactgt tagtacattg aatcctctag atgcaagtgg agctgctaaa aggtctagag
     8221 cgtctaaatt tccatcagaa ccacctagca ttaaaattgt tttattatta gaattttgtt
     8281 tataaaataa ttttcctata aactcatcag atatatattt tgtggtaata tcttctgatt
     8341 taaatattct ctttaattca acttcttttc tttcaataga agtttcaaat atcatattca
     8401 taattattgg tttatcaata gatatatttt ctgcaaaatt tttaccttca gttttagatt
     8461 tttccagcga ataaattaac cccatacaat tatcagcctt ataagtacct tctataggtt
     8521 caactaaatc taaatctact tctccttttt catttgaaca aaacactcca taagatgaaa
     8581 attcttctcc cttgcaccat ggaaattcca ttttcacact tattttaagt ttagtatttg
     8641 gaattaagtc cgaaaccata atcttaagtc tttcatccac aaatgattct acattgtata
     8701 catttacttt cataatctat ttctccttat aataattatt tatgttttat ataaaacagc
     8761 tttatctaat acaacataaa taaattttta aattaagaca ccatattgac tatttcattt
     8821 tatcaacata ttctattttt gtcaacatat tgtctttttt agcaatttaa aattatgtag
     8881 tctatatgaa atccagttga tttttggcta attaaagata ggaatgctat taggtttaga
     8941 atatatatat tcaaaataaa aaaccactca ttttaagaat ggtttttaat ataaactatt
     9001 tgttattaat aaggatattt ttttatttaa atttatataa tcgaacatct aatatttaat
     9061 gtttaataaa attatgcatt tgcaaaagta tatatggcat taactcttgc tacatataaa
     9121 ttctcatcac tacatccaat atatctagca tatttttcaa tcttttcttt tgcattttca
     9181 acatcatttt catcgctctc cattaactca tttatataaa tatccattaa tgacgcttta
     9241 tattctctta tatcatagcc taaaaattca gtcaatctct caatttcctt actaaaatct
     9301 ccatcttctt ttaccaaaaa ttcgtataca tcttccctat atggatttaa agttattatt
     9361 tgttttacta ctttgcattc gtcagccttc ccaataaaac catctttata attattaaat
     9421 aaggcattag atttttttat agattcttca tcaatataag aatgtaccgc agttatacga
     9481 ttttctataa gggcatctac aacagcataa tgaattttaa aacaatcatt gtataaataa
     9541 ttgctcattt tctttattgt cgattctatt tctaagaagc tcgcgtggcc atttttttta
     9601 tttttatttg gtataataaa agttttattc aagttattaa aaagtctctt aaaatctaaa
     9661 tatttttcac aataaacact tttaaaggta ttgatatcta tagttataat tccaaaactt
     9721 actatagtct caattccttt cttaaaagac tcttctatat atttttctcc taaacttaaa
     9781 cctctatcag atatttgctt tagattttga aaactattta aacaatctct ctcaaagttt
     9841 ttagatattt ccaaagcata taatttaaag attttcctta tcttattata attatttttt
     9901 gtatcatcaa atctgataat agctccattt acatcaaaag taatcattga gttttaacct
     9961 ccccattagt ttcatcaata aaataaattt aatataaact ttataatcat aatattaaac
    10021 ttatttatac tataattatt aatttgatga taaaagctat tttccttcta aataaaaaat
    10081 ttgtctaaag tttctaaaaa atttagacaa atcttaggtt attactcttt aaattatttt
    10141 taaattattt ttaaattatt ccaaatacgc caaataatat tggtataaat atagctggta
    10201 acaaattggc tacttttata ttcgttaatt taagtacatt tagaccaagt cctataatta
    10261 ataaacttcc agctgctgtc atttctgtga ctacaggatc acttaaaaat ccatttaaaa
    10321 atgatgctcc cacacatata ataccttcat acaaaaatac agatattgca gaaaacatta
    10381 cacctatacc taaagaagca gtaaatataa ttgaagctat tccatctata acagatttta
    10441 caaataaagt atcatgactt cctgtaagac cactttctaa tgaaccaact actgccatgg
    10501 ctcctataca gaataataaa ctagatgata taaaaccctc tgctatagaa tcttttttat
    10561 ttcctttaga aaatttactt tgtaaaaaag cacccaactt atttacccat ttgtcaatat
    10621 ctattaattc tcctatcaga gcacctacag ctacggatat tattgtcact aatgtgtttt
    10681 ttccttctag tgctcctgat ataccaatgt acaatacaca taaggcaatt ccattcatta
    10741 tagtatcgct tactctttga ggtaatcctc cttttattat aagacctact atacatccac
    10801 ctgcaattgc tagactattt gctattactc caaacatttt ttctcccctc gattagctaa
    10861 ttgaaattta tcctattatt aaaacctaaa catctatact ttgtattgga tattttaaat
    10921 attccaaaaa ttcttcaaaa ccttttctag atatatttac agttttagta tttatgttag
    10981 gatgaaaatt taagtaatct tctttaatta catctttatc tatgtaaagc tcaacttttc
    11041 tttctgtatc atttattaat gaaaaaggat ttacacttcc aggaagaagt ttaagtacat
    11101 tgtatagtct ttcaggtgaa gcaaacgata atctagaact tcctatctta tctttaagtg
    11161 agtttaaatc aacttgtttt tctcctttaa ctacaataag ataatactta tttcctttgg
    11221 catttcttaa aaataaattt ttacattgac acccctttgt tattccatct aaaatgacta
    11281 attgctctgc tgtatataca gcttcatgct ctactacatc atattcaaca tttaatttat
    11341 ccaaaatatc atatatttta gtttcattca tgctagattc attttcatct tgttcttttt
    11401 tattatttaa atcactcata cttttctcct taaacacttc atcataatat cattgtgagg
    11461 tattattcta ctatagctat tgctcttact ggagctccat cagcatcatt atacttaaat
    11521 ggtgctgcta taaatagaaa tttttctgga acattttcta aatttgtaag attttctatg
    11581 attatttttc ctttttcaaa taaaatatga tgcacttcaa atactgatgt gtcatatcta
    11641 tccactgata gcatatctat tcctatgcct tttatattag tattagctat atagtttgca
    11701 gcttcttttg ttaatgtagg ataacctaca tagtactgct ttttatccca aaattttgac
    11761 caacctgatt taaatattat aaaatcacta ttttttattt catcttcata tttttttaga
    11821 tattctaatt ctatattctc atttcccttt tcaagttcca atacataagc tttcccaaca
    11881 aaattttcta tatccaactt atctagggtc tcacctttag tatacatatg tcttggtgaa
    11941 tccatatgag ttccattgtg agaaaataca ctaattaatg tttcttgata accattttct
    12001 tcaataatag ctactttctt gatatctggt ctattaggct ctgcatatac aggcatgtca
    12061 ttatgtgtca catgagttaa atcaaaaacc ttcatcttta tataccccct atgtttttat
    12121 tttctattat atacctaaat tcattttatt tctatcagat tttattccat tatataccat
    12181 actactatat attttttata aacctcaagg gatttaccga aaaatcttgg aaagaattat
    12241 cagtaaatat aacatcttca cccaaaaaac gtttatacct tccttcttct ttctttaaag
    12301 taattctctg tttttgtgaa tgattattaa aaaccattat cttaagagga ggtttttttc
    12361 tattagtctg cactcttttt aacatgtatt ttatgtgcat atcagcttca ggaaatgaat
    12421 atccacaaaa tattaaaagg tcagtatctc ttaaagtttt ttctgtttca ttccaaacat
    12481 tacttaaaaa tatgtttgac atattcttaa aatatgttgg tggcactata ataggttctg
    12541 ttaactcacc acactctaaa cattttgtct ctgaactatt ttcaataagc ttcatgacac
    12601 caccttcatg tggtgtaaga gttacactat tacaaatggg gcaataaagc caattcaatg
    12661 acccatgtat cttatataat ttgattaatg gttcttttgg ttttctccaa tttaactcct
    12721 ctttaaaatt tgtaaactcc acaccataat caagcataat tggcaaatca ttcttatctc
    12781 ttaatccagt aactgtattg tctatatgta tatcataatt tgcactaata aaagtagtat
    12841 caagaagcaa atcattttcc aaaagatttt caactaaaag tttatgatat ttattatctg
    12901 ttctaggtgt attatgaatt gcttcagcca ttagcagtat taaatattgt cttaaaaaac
    12961 gtatactatc acttctgtta tttagatttt ctagtccaaa atttctaaat gcttcttttc
    13021 tttgttctgc taagtctaaa agacctaaaa cttcttcaaa ggtaggaaaa tttgcattat
    13081 ctatattatc ttttactata tcaatattga acatctgttt aaagaaaata tacaattctc
    13141 tattcatttt ggtattataa tttgtgggat ttagtttttt aaaatattcg ctaaaaagtt
    13201 cattttgaat aggtagattc tctgctgctg aagctcctgc tcctaaaaat attgctattt
    13261 tcatatagtt cctcctaata taaatattac tcctattttt atatccatta aatatccata
    13321 tcatttattt tatgatacaa caataaagct gtcttttact agaaaaaatc ctattacaag
    13381 acagccttat attatattaa atctttaata attcgtaaat agaaagagca tatatttgag
    13441 tagcttttat aatatcctct actattaagt attcattagc ttggtgttct gtttctggtt
    13501 ttcctggaaa aatagctcca aatgcaacac aattatctaa agctcttgcg tatgttgctc
    13561 ctccagatga aagtggtgtt ccatcaagac cagtttcttc ttcatatact tttctaagtg
    13621 tcttaacaag aagagtatct tctggaacat agattgaatc caagaaatca tattcttcat
    13681 aatttaaatt gtatttatct gtcattttct ttaactcttt tacaaaatca tcctttttgt
    13741 atgttactgg aatccttaca tctattcctg caaattcttt ttcgttatca atagttactc
    13801 tacctatatt tactgttagt ttacctgata catcatcctt gcagttagga attatattgt
    13861 ttccattagc atcttcacca ataacttctg ctaaaaactt aattatatta gaatctatac
    13921 ctatattatt aagagctata caaagtctag ccactgcatt tataccagta tcacttgctg
    13981 ctgaatggac actctttcca attatacaga ttttattacc ttcaacagta tactcaaaat
    14041 ttaatttatt aagttctttt tttaatttat cagagtattt tccaatatag attgctttac
    14101 ctggaactgc atttaaagcc tttccaactg acaactctat atcacttttg ctatcacaag
    14161 tcaaatgaac ttgtaaaaga cctttctctg catttgttat tgggaatcta gaatctggag
    14221 taaatccata gtttggtatt tcttcattat tttctttata tttatttata catctccaaa
    14281 gattttcttc atcagcccca aaaatgaatc ttatcctctt attaaagtct acatttaaat
    14341 ccataagagc tttcactgca taaattgctg atattgtagg tcctttatca tcttgtgttc
    14401 ctcttccata caatttacca tcttctaaaa cagcttcaaa tggagggtaa ttccagcttt
    14461 ctaaatcacc ttctggaacc acatcaacgt gaccaagtat acctatcatt ttttcacctt
    14521 gtcctatttc tgcatatcca taatatccat ctttgtatac tgtcttaaat cctaaagatt
    14581 tacataaatc taatatacct cttaaagctt tatctacatt ttcaccaaat gggcaatttt
    14641 cagttgcttc agatataaca ctaggtattt ttacactttc ttgtattgaa gaaatcattt
    14701 catcttgtag agaatttact ttttctttaa tttgctcttt catattatca cccttcaatt
    14761 ttcatcataa tatactaata aatattttga ccttttaaaa aaaaaatatg cttatttttt
    14821 cagttttttt ataaattcaa tcgcttttaa agtccccatt tttgaatggt caaatgtaag
    14881 tccaccttgg aaataaacat tataaggagg tcttattgga gcatctgcac ttaattctat
    14941 agaagaacct tgtataaatg ctcctgctgc cattatgact tcactttcgt atccaggcat
    15001 gtcccaagga acaggtttta cataagaatc gactggtgca gcttcttgta taccttcaca
    15061 aaaacttatt acttcatcag cattattaag tctaatacat tgaataatat cacttcttaa
    15121 gtcatcatat tttggtaata catcatatcc taatttttca aaagctcttg agcaaaagat
    15181 agctcccatt acagcctgag atactatata aggcgccaag aaaaaaccct gaagtacatt
    15241 tctagttgtt ccaaaagtaa gaccacattc ttttcctatt ccaggagaag tcattctata
    15301 agatataagc tcaatcaaat cttttctacc agctatatat ccacctgtca atgcaagccc
    15361 accaccagga ttttttatta aagagccagc cataacatct gctccaacat cagtaggctc
    15421 tttggtatct aaaaactcac cataacaatt atcaaccatt actatagctt ctggtttaac
    15481 agactttatt acttctatag cttcttttat atcactaatt gaaagtgatt ttctccaaga
    15541 atatcctttt gacctttgta tcataacgag tttagttcta tcatttattt tgtttttaat
    15601 tccttctaaa tctaagtttc catcctctaa aaaatctaca tcatcatatg taacaccata
    15661 ctctttcaat gaaccttttt cttctcttat gcctataact ccctctaaag tatcatatgg
    15721 tctaccagta acagataaaa tttcatctcc tggtctaact ataccttgta tacataaagt
    15781 aagtgcatgt gtaccattta caattattgg tcttacaaga gcatcttctg tattaaatac
    15841 tttagaataa atttcctcta ttttttcacg accaatatca ttatatccat atcctgtagt
    15901 ccagttaaag tgcatatcac ttagtttaga ttcttgcata gcttttaaaa ctttatattg
    15961 attatattct cttatttctt ttatttcttc aaacttatct tttatttctt ccattatttc
    16021 ttgcgatagt ttaaaagtat catcatctat tccatagtag tcttttaata attcttttgt
    16081 ctcattaagc attttatcac ctgtctcttt attattttat ttaaacatgt aaaggagact
    16141 attttactaa aacaaataaa ttatagttta taagttagta taagtctcct ctcaatcatc
    16201 ttaaattaaa ttaattatct atctgttgtt attattattg ttgttttggt tttgatttgt
    16261 tagattaata tactttccag gttgtatagt tgatacagca tgtttataaa tcatgttttg
    16321 ttgtctatta tccccttcta ttaatattat ataactatca aaccccttta caagcccttt
    16381 aacttgcact ccattaacta aatatatagt aacaggtatc ctttctttcc ttgcattatt
    16441 taaaaacaaa tcttgtaaat ttaaaactgt atttttcatt agatttgtac cctcccaaga
    16501 ttaaattgaa cttctaaaat attcctacct tatattctat ataagttatg acaaattttc
    16561 aatattaagt tgtatactta aaaatttata tattatttta tagaatcttt aatatataag
    16621 attatttcat tttttaattc gtctatattt tcatattggt ctaaatcaaa ccattttgct
    16681 gttttatatc ttttaaacca tgttatctga cgttttgcat accttctaga atctctttta
    16741 attatttcaa tagcttcttc gtatgtatat tctccatcca gatactttaa tatctcttta
    16801 taacctatac cttgcattga tatcatatct tttttaaatc ccatacttaa aagttttttt
    16861 acctcttcaa ccaatccatt tttaagcata atatcaactc tcatatttat tctttgatac
    16921 aaatgttctc tatctctatt tagaactatt attattggtt gatattcttc attaaatttt
    16981 aaatcacttg aaaaatcatt cattttctta ccacttagac aaacttcaag agctcttatg
    17041 acccttttag tattgttttt atggattcta ttagcagatt cttcatcaag ttcttttaac
    17101 ttattatgca tgtaatctat tcccttttca gcaagttgtt tttgaagttc ttctcttaat
    17161 tcattattgg catcagattg tgcaaaatcc atattatata ttaaagagtt taaataaagt
    17221 cctgtgcctc cagttactaa aacacatttt tcctttttat ttatttcatg tatatatttt
    17281 tttgctcttt gttgaaactc tgaaactgaa aatggatagt caggcttaac ttcatctata
    17341 aggtaatgct tcactccttg catctcttcc tctgtaactt ttgcacttcc aatatccata
    17401 tattcatata tttgcataga atcagctgaa attatctctg cattcatatc ttttgctaat
    17461 tttattgaca aatctgtttt tccaacagca gttggacctg taagtattat aagtggtatt
    17521 tttttcataa cattcctttc catatatttt aaatatccaa atattttaca taattctttt
    17581 aaacattttt tctatttctg tcttagatat ttcgaccatt ataggtcttc catgaggaca
    17641 tgtaaatgga ttctcacact tttctagctg ttccaataaa ctttttattt caatatcgta
    17701 tattttatca tttgctttta tagcagacct acatgccata gatgcaaatc tctctccttt
    17761 taaatcataa ttacttgtaa tttcttctat attatcaatt atttgaagta taaatttctc
    17821 ggtttcaggt actccaaata tagtaggaac acatcttacc attatatgat tattgccaaa
    17881 aatttctagc tcaaaaccaa atttcataaa taactccaag ttattttcaa tttgaagcat
    17941 atctacattt gatacttcaa taaccactgg gtctaaaagt atttgcatat ttatatcttg
    18001 tctatagaac ttctccatat atctttcata tagtattctt tcatgtgcag catgttggtc
    18061 caacaaatac atagaatcgt ctttgctcaa aattatataa gtattaaata caactccaat
    18121 tacagaatat ccgtataaag aaaacttgcg ttttgaacta cacaagtatt cttcttcact
    18181 gtccttaata ctatctccta agtaataatt tccttcattt ttaattccat caacttgaaa
    18241 ctcttcttgt atattatcat ttgcactatc ttcacttaga taattaatgt tttttacatc
    18301 atcttctaca cttgcattta ggacttcact tactgattga aattcattta ttggtttttc
    18361 acttgataaa gtaacaactt caatgacttc atccttacct ttagtaatat tactattttt
    18421 aggagtagat tctaataaat cattatttct taatttataa tctgtacttt tttctctact
    18481 gttaatagcg attctaggtt gtgttttttt atcactataa gtagcatatt ttccaataag
    18541 atttgagtgt attaatttaa cctttaaaaa atctctaagt tctatataca cttcttgttc
    18601 tttttcaaat ttaatttcaa gtttattggg gtgtatattt acatctatac atgatgggtc
    18661 tacttctata tttaagaagc acactgcatg tttacctatt ggaattatag acttataact
    18721 ttctgttatt gcatctatga ttattttact ttttacaaat cttttattta tatagatatg
    18781 ttgtaagttt ttatttgaac ggtatatatt gttattacct atatatccat tcattttaaa
    18841 gtgattgcac ttaaactcta catctattat attttccgtt atttcttttc catatatact
    18901 tctaatagtg tttacaagct ttccatcccc tggagtattt agcatctgtt tattgttatt
    18961 tgtatattta aattgaatgt ttggattacc tattgcaagt ttatttatca aatcacttat
    19021 atttatagtt tctgcatgag tagattttaa aaatttctgt cttgctggtg tattaaagaa
    19081 aatatctttt ataatgatgg tagttccatt agtagaacct attggttctt tcgatattat
    19141 ttttccacct tcaacatata ttttagttcc tattatttca tctttagttt tagttgtcat
    19201 ttctaatttt gatacagctg aaatagatgc caatgcttct cctctaaaac caagagagta
    19261 taaatcatac aaatcatcaa tttttttaat tttactagta gcatgtctca aaaatgattt
    19321 ttcaacttca cttgaaggta ttcctattcc attatctgta atcttaatta gactttttcc
    19381 tccatcaatt atatctatgg aaattttatt tgcacctgca tcaatggagt tttcaataag
    19441 ttcttttact acagaagaag gtctttctac aacttcacct gctgctatct tatttatagt
    19501 caaatcatct aggatgttaa ttatattttt catttttaca cctctaagat tttatttctt
    19561 ttgcttttct ttgaagatta taaagagagt ttatagcatc taatggagtc atatttaaaa
    19621 tatccagatt taaaatttct tctgatagta tatctctatt tacactatca aaagagattt
    19681 gagttaaggc tacttctttt acagaatcca cttgattaat tttctcttta tcatcatttt
    19741 gctttattaa agcaacttca tcatttaatg ctttaaattt agaatttaat ttatcataat
    19801 ccttttttat atgtttgtag tcttttacta taagttcatg ttctattttt agagattcat
    19861 atttattctt aaaattactt tgatttacct taactaattc ttcgtccaaa ttactattaa
    19921 ttatattatt gttttcttta tctccattta ttgcaacact gttgtaaaca tgattttttt
    19981 ccaagtcttt aagtatatat tttgctcttt ctatgacttc gtctggtaat ttagctaatt
    20041 tagcaacata tattccataa ctcttgtctg ctccctgagg aattattttt cttaaaaata
    20101 ttattccttc cccatcttct ttgactgcta ttgaataatt tttaacctct ttaaactctt
    20161 cttctaaatc agtaagttca tgataatgtg ttgcaaataa tgtcttacat ctaatatttt
    20221 tttgaatata ttccactatt gaccatgcta aacttatacc atcataggta ctagttccac
    20281 gacctatttc atctaatata actaaacttc tctctgttgc attttttaat ataagggata
    20341 cttcgttcat ctctaccata aaagtagatt gaccttgaga taaatcgtca ctagctccaa
    20401 ctcttgtaaa tattctatct aatattggaa tatcagcaga ctctgctggt acaaatgagc
    20461 ctatatgtgc cataagagct attatagcag tttgtctcat atatgttgat ttacctgaca
    20521 tattaggacc agttattatg ttaataatgt tttctccacg atttaaataa gtatcatttg
    20581 gaacgaaatt ttcctctcct actatatttt cgacaacagg atgtctacca tttcttatat
    20641 caagtttatt attttcattt attgctggtt ttacatagtt atttatatgt gcaactgttg
    20701 caagggacac aaaaacatct atattagcta tagtcttagc cactttttgt attctatcta
    20761 tgtttttata tattgtatct cttatctcta caaatatctc gtattctaaa gatttaatct
    20821 tttcctctgc atgtagtatc ttttcttcta tttcctttaa ttctggagtt atatatcttt
    20881 ctgcattact aagtgtttgc tttcttatat atgtctcatc tagctttgcc tgtttaaagt
    20941 tagcttttgt tatttctata taatatccaa aaactttatt aaagcctatt tttaatgatt
    21001 taactcctgt tttttctctt tccctgtttt ctatttcctt tacaagaaag gctccattct
    21061 tagaaatctc tcttagttcc ttaagttcat cactaaaatc tgatttaatt atgtttccat
    21121 cttttatggt tatcgtaggt tcttctagaa tagcttcatc tattaaattg tatatatcat
    21181 ctaatttatc catttctgaa acatactctt ttaggatttt tgcatttgat aaatttatag
    21241 tatcttttaa attaggtaac ttttctatcg aattttttaa gtgaattaac tcttttggtg
    21301 ttactctctc aaatgctatc tttccacata ttctttctat atcgtatata tttttaagta
    21361 tatcatttag gtcttctctt aatataaagt catcttttat ttcttctatt acatctaatc
    21421 tattttcaat tttagattta tttatcaaag gttcttctac atattttctt aaaagtctac
    21481 ctcccattgc tgtagaagtt ttatcaagta cgtgtaataa tgaacctttt ttcttattcc
    21541 ctcttatagt ttgagtaagt tctaaattag ttctagtaaa catatctaga accatatatt
    21601 ctgatgaatt gtatatattt atattattaa tgttagatgt tatttgtttt tgagtattgt
    21661 atatataatt cagaagaatt gataagcttg actttattag acctttatca tcaaacttta
    21721 gtttttgtag ataaacatct ggaaaatatt cttttaatat atttatatcc aaatagttat
    21781 cactaaaact ttcatttatg tatatattac ttacagtagc tatatctcta agattttcta
    21841 taaagtctaa atcatttata attatttcag ttggatggat ttttgcaatt tcttcaatta
    21901 ccttatcttc atttaaacat gttgcattag tttctcctgt acttatatca acataagtta
    21961 gacctatatt agtaccatcc ttatacaaag ataataaata attattcttt ttattttcaa
    22021 gtaaatttcc atctaaaact gtaccaggag tgatgactct tataacctct cttctaacaa
    22081 ttccttttgc tgtagatggg tcttccattt gttcacctat tgctacttta tatccatttt
    22141 caactaattt tgatatatat gaattggctg agtggaaagg aacaccacac ataggagcac
    22201 gttcttctaa accacaagct tttcctgtca atgctatttc aagagctttt gaagctacta
    22261 atgcatcttc aaaaaacatt tcataaaaat ctccaagtct aaaaaacagt atacagtctt
    22321 tatatctatt ttttacttca agatactgtt tcatcatagg agttaattta ttcatatcta
    22381 tttgcattat caaacctcct tcttttctaa ttaaatatta tatcatagta gatgattatt
    22441 attaacacaa tatataaaat tttgacatat aactaaaaac aatccaaaat gtatactcac
    22501 attttggatt gctaagttta atgcttatta aataaaaata ttttttttac aataaagata
    22561 ttatttcttt tttaagttta atgatatttt caaggttatc ttcatttaac tttctactat
    22621 ctataacaaa ttctttcttt actattgatg gtttttttga aataacataa attctgtttg
    22681 atagcattat agcttcgtca atatcatgag ttataagaag tattgttgaa tttacttttt
    22741 tcttaacatc taaaagccat ttttgcatgg atgttttagt taatgaatca agtgccccaa
    22801 aaggctcatc taaaagcatt atgtcattag aatttataaa agttctcaag aagttagctc
    22861 tctgtctcat tcctccagaa agttctgaag gatatttatc ttcatatccg cttaatccaa
    22921 acacctcaaa ataaggtttt accattgacc tagctttact ctttttttct ttttttaaaa
    22981 ttagtggtag tgacacatta tctattatag ttttatatgg caagagtaaa tctttttgat
    23041 acatataact atacttacca tttatattca ctttgccact atcataatct gtaagtttag
    23101 ttataatatt aaaaatagta ctttttccac atccacttgg ccctaatata gaaacaaact
    23161 caccttcata aacatcaata cttatatctt ttaaaacttc aatatcttta taagtttttg
    23221 atacattttc tatagatact tttacttttt tattgtggta aaaattcatt tgtgtaagca
    23281 tcacttgcct tcatatcctt atctattaat ttatattctt ttaagaatga tgtatagttg
    23341 ttccatacac tatctttcat ttctccccat cgtggtgcat catctttgta cttgcttgct
    23401 aaatattctt gactctttaa agctagtttt tcatctactt caggtgcatg ttttactaaa
    23461 atttttgctg attcttctgg attttttatt gcatattcat aaccttttgt tgtagctttt
    23521 aagaattttt tagcaagctc tggattatct ttaagcacag tttcattaga aattataagt
    23581 ggtgtataat aatccaatct ctcatctaaa tcttttactg gtataaagtt taagtcatat
    23641 ccaattaaat cagctttaac tgcatcccaa ccttcaaata tccatgcaaa atctacagtt
    23701 ttcatagctg caaagaaatc atcttgacca gtattaacta tctttaactt attaaaatca
    23761 gctccatttt tcttcataac tgctttaaaa acagcttctt ctgatggaga gccccaacca
    23821 ccataagttt ttccttcaaa atctttagct gttgttatat tcttttcttt tggtgatgca
    23881 aacccagaag tattatgttg tataacagtt gctacagctt ttattggaag tggatcttca
    23941 ctagtctttg cataagtaac ttgttcttga tagcttatac caaagtcagc ttttcctgtt
    24001 gctactaaag tttctgctcc actttcaggt ggttgtacta tttcaacatc caaaccttct
    24061 tctttatagt aacccttatc aagtgctaca aataatccag tatgatttgt atttggtgtc
    24121 caatcaagca ccatagttac ttttttagaa gaagtatctt tatcattgtt tttgtcttta
    24181 ttgttatttg aagaacatcc agttattgtt gccaatgtaa aaacacttac taaacctaat
    24241 attcctaact ttttaaaatt cataattgcc tccctcaaat acctttaatc tattaatcta
    24301 tgtcttataa caactttttt tacagcctgt acaattccta caaacattaa actaaatata
    24361 actactaata tagtagatgc aaatacttta tcaagtgcat atgcactttt tgacctaagc
    24421 atataaacac caagtccttt cgttccacct agccattctg caacagttgc tgccattaca
    24481 gcatatgttg cagatatttt aagtccagaa aaaaatttat ccattgccat aggaaattta
    24541 aggtgaatga atgtattaac tttatttgag tccatagtct taaataaatt taaataatct
    24601 ttatcaatat tctcaatgcc atctacaaaa cttattaata taggaaaaaa acaagtaagc
    24661 attaccatca atacttttgg cattgttcca aatccaaacc atatcataaa gagtggtgct
    24721 ataattattg taggtatcat ctgtgtaacc aacataatag gataaagaca tttttttatt
    24781 attgatacaa aatccatcaa tattccaatt accaatgata ttataattgc gactataaaa
    24841 ccaagtattg cttctcctat agtaaccaca gcatgcatgg ataaattttg ataatcttta
    24901 aaaaacacat taattatatc tactggtgtg ggtagtatat actgaggaac ttctaaaact
    24961 cctaccatag attgccaaag aataagtata aacaaaaatg ttgctattgg atatatacta
    25021 tctttgactt tatctatgat ttctaagttt ttcatctata gtcagacctc cagctttttc
    25081 atctactcta gctactgtta tgactctttc acatccaaga ttaaaaggga ttttatgtac
    25141 cttttttaat aattcaaaaa cttcatcaaa atcaccttca acagtagtgc tcattgctcc
    25201 tatttcacat ttaagaccac ttttttcaat ttcagcaata caagcatcta ctactttata
    25261 catctcttct tcacctttgt aaggtcttag tggcattact gctatgtctg cgattaccat
    25321 ttaaatcact cctttaaaaa taattaatac ctactaggta tattttgttt tttattaaaa
    25381 aattttatta ctaaaaagat attatattta aataaaagaa aagtcttatc caaaatagga
    25441 taagacttat ttttatctta actggtctcc ctacactggc attatccaaa caggttcaaa
    25501 gggtcgagat aaaatctcct ctcagcacat tagctccccg aaatatatta tatcttttct
    25561 acttgattat actacactta tcatatttta acaaattttt tatttatatt agatatttta
    25621 tccgttcaaa aatatctaat ataaattgta cattattaca ccaaataaat tatcttaaaa
    25681 tggtatttta tcaacatctt ccaaaactag attaactgaa ttttctagtt tttattttgc
    25741 tatttcaagc atatttggat gtatatcaat tccatacaaa tattgtgcta aatttctgtt
    25801 ttcactcatc taatttgcaa tcgttacaga ttacagatta gaaacagttt ttagactcta
    25861 cttatcttta tctacaaaaa atctttcaat aaattcatta ataatattca gtaatctaaa
    25921 aataaacctc tatctggatt tttaagatac aaacctttta ggaagtatct tttgtgataa
    25981 tataaaaatt actcgtcatt atcatcaaaa tcctcagcaa ccaacatttt atataagaat
    26041 caaactctat aatataatta ttttatccaa tttatttttg agaaataaaa tattttttta
    26101 atttttaaat tttttaatat gcaccttgaa aaaagaaacg ttttcctata ttatggtaat
    26161 agtagaataa aactaaatag gcaaaactag agaaatttag tgacgcaaag ctatagggac
    26221 taagacttat gaaatgagtt atgtcagcca gttgccaaag agatattttc tttttgtttt
    26281 tatgaatgtt ttttaggggg atatatgata tatgtttatg gataaaatgg atagatgtac
    26341 acatatacta actgcatata tatgcagttc acgcgattac tgcaatttta tagatacaca
    26401 attaaatgat tttatactag aatatggaga gaatgtggta gaatcttgtt tgcatcaagt
    26461 gatggtattg gtaagcagat ataattaaag aaattttatt ttctagaaaa ccctttttag
    26521 ttttatctac gttttataat tcattaaaga actaattaaa ataagcttca ttatatattt
    26581 ttcatgaagt aaacagttaa gtttatctca tatactgttt accccaatta cataattcta
    26641 ataatataga ttccaatgat ttaccacgtt tagtagacaa atattcaact tttggaggaa
    26701 tagtcggata agaaatcctc ttaataagtc catctctttc aagatacttt aattcttgtg
    26761 acagtatctt atgacttata tgaacaactt ctttttttaa ttgactgtaa cgtattggct
    26821 cttgttttaa aatatgccat aaaataattg ccctccattt acttcctatc acagtcatgg
    26881 taaaaccaat tgaacaatta aaatcttttt gtctttcttc aatatttgga aattcttcaa
    26941 aattattatc tatatccatc tcaatcctct ctttcaaaat agtaagtatc taactaaaaa
    27001 gtgcattctt gtttagcgta ctaattcgta atataatctt gattaaaaaa tagctatgca
    27061 cactcgtgac tttagtcatg ggaggttcag tgtagcatag agccttacat aatactactt
    27121 ttttagatgt atatgtttaa attttaatat agacatatgt tattttatta attttattta
    27181 aacagataat aataaataat aatttatctc aagttttcta tgtagataaa ctaatctaat
    27241 gtttagaaag gattatataa tatgaataaa aaatttcgtc tttatcaagt tgactcatta
    27301 caaaagaaaa atttacaggt aatccagctg gtgtaattac aaatgctgat ggcttatctg
    27361 aatcacaaat gcaaaaaata gcaagagaat taaataattc agaaactgct tttattttca
    27421 ataacttggc taaaaatcat caagaaaatt atgatgtaca tatcagattt tttacaccaa
    27481 ctaatgaggt tcctatatgt ggtcatgcta ctattgctgc ccattatgtt agagcaatag
    27541 aaaaccaatt caataacaaa acagtatttc aaaaaactgg tgctggaata ttaccagttg
    27601 atattataaa aaaagataat gattatgaaa taattatgac acaaggaaaa attgaagtat
    27661 ctccaccatt acaacaaacc tatcaaaaaa gattacttaa tgcatttggt atttaaagga
    27721 agatattcgt acagattgtc ctattgccat atcatctaca ggacattcaa aagttatgat
    27781 tggaataaag gaattatcaa cattaaatag tttatctcca aatttatatg aattatcgaa
    27841 aataagtcaa gaaataaaat gtaatggata ttatattttt acattacata ataaagaaac
    27901 tctacaagtt catggaagaa tgtttgctcc tgcaattggt atttcagaag accctgttac
    27961 aggaaatgct aatggaccct taggtgcgta ccttgtacat tataaagtaa tacctacacc
    28021 caacacagag tttatatttg aagcagttca aggagaagca ataaaacgca aaggcaagat
    28081 gttagtaaaa gtaattattt tagataataa gccagttgaa gtaaaaataa ttggaaaagc
    28141 tgtaattgca ttctcaacaa acatagaaat ctaattatag attccttcta agtaaatttt
    28201 tgaacttttt aatactaatt taataacaaa ctaatttata tacaatcaaa ttaatttttt
    28261 tattatttgt ta
//
LOCUS       DAELTA010000036        27853 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.036, whole genome shotgun sequence.
ACCESSION   DAELTA010000036 DAELTA010000000
VERSION     DAELTA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.036"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(25..516)
                     /locus_tag="KPG13_001346"
     CDS             complement(25..516)
                     /locus_tag="KPG13_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985082.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(527..970)
                     /locus_tag="KPG13_001347"
     CDS             complement(527..970)
                     /locus_tag="KPG13_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985083.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(960..1799)
                     /locus_tag="KPG13_001348"
     CDS             complement(960..1799)
                     /locus_tag="KPG13_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985084.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(2018..3403)
                     /locus_tag="KPG13_001349"
     CDS             complement(2018..3403)
                     /locus_tag="KPG13_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0985085.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CLVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(3576..4319)
                     /locus_tag="KPG13_001350"
     CDS             complement(3576..4319)
                     /locus_tag="KPG13_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG0985086.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(4339..5601)
                     /locus_tag="KPG13_001351"
     CDS             complement(4339..5601)
                     /locus_tag="KPG13_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0985087.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(5785..6783)
                     /locus_tag="KPG13_001352"
     CDS             complement(5785..6783)
                     /locus_tag="KPG13_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG0985088.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(6767..8278)
                     /locus_tag="KPG13_001353"
     CDS             complement(6767..8278)
                     /locus_tag="KPG13_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG0985089.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(8332..9294)
                     /locus_tag="KPG13_001354"
     CDS             complement(8332..9294)
                     /locus_tag="KPG13_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0985090.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(9342..10232)
                     /gene="rbsK"
                     /locus_tag="KPG13_001355"
     CDS             complement(9342..10232)
                     /gene="rbsK"
                     /locus_tag="KPG13_001355"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG0985091.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(10274..11287)
                     /locus_tag="KPG13_001356"
     CDS             complement(10274..11287)
                     /locus_tag="KPG13_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0985092.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(11516..12493)
                     /gene="bioB"
                     /locus_tag="KPG13_001357"
     CDS             complement(11516..12493)
                     /gene="bioB"
                     /locus_tag="KPG13_001357"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG0985093.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(12699..12962)
                     /locus_tag="KPG13_001358"
     CDS             complement(12699..12962)
                     /locus_tag="KPG13_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985094.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(13032..14105)
                     /locus_tag="KPG13_001359"
     CDS             complement(13032..14105)
                     /locus_tag="KPG13_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0985095.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(14353..15111)
                     /locus_tag="KPG13_001360"
     CDS             complement(14353..15111)
                     /locus_tag="KPG13_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985096.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(15108..16025)
                     /locus_tag="KPG13_001361"
     CDS             complement(15108..16025)
                     /locus_tag="KPG13_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985097.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(16025..16225)
                     /locus_tag="KPG13_001362"
     CDS             complement(16025..16225)
                     /locus_tag="KPG13_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG0985098.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(16230..17324)
                     /locus_tag="KPG13_001363"
     CDS             complement(16230..17324)
                     /locus_tag="KPG13_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985099.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(17834..17974)
                     /locus_tag="KPG13_001364"
     CDS             complement(17834..17974)
                     /locus_tag="KPG13_001364"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG0985100.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(18284..19591)
                     /locus_tag="KPG13_001365"
     CDS             complement(18284..19591)
                     /locus_tag="KPG13_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0985101.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(19656..20135)
                     /locus_tag="KPG13_001366"
     CDS             complement(19656..20135)
                     /locus_tag="KPG13_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG0985102.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(20182..21045)
                     /locus_tag="KPG13_001367"
     CDS             complement(20182..21045)
                     /locus_tag="KPG13_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0985103.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(21045..21800)
                     /locus_tag="KPG13_001368"
     CDS             complement(21045..21800)
                     /locus_tag="KPG13_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0985104.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(21827..22315)
                     /locus_tag="KPG13_001369"
     CDS             complement(21827..22315)
                     /locus_tag="KPG13_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985105.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(22333..22734)
                     /locus_tag="KPG13_001370"
     CDS             complement(22333..22734)
                     /locus_tag="KPG13_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG0985106.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(22737..23234)
                     /locus_tag="KPG13_001371"
     CDS             complement(22737..23234)
                     /locus_tag="KPG13_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985107.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(23258..23662)
                     /locus_tag="KPG13_001372"
     CDS             complement(23258..23662)
                     /locus_tag="KPG13_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0985108.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(23892..26459)
                     /locus_tag="KPG13_001373"
     CDS             complement(23892..26459)
                     /locus_tag="KPG13_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985109.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            26625..27368
                     /locus_tag="KPG13_001374"
     CDS             26625..27368
                     /locus_tag="KPG13_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0985110.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(27680..>27853)
                     /locus_tag="KPG13_001375"
     CDS             complement(27680..>27853)
                     /locus_tag="KPG13_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985111.1"
                     /translation="KKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKELNEYGYQVGKI
                     SLKEKTVIFDKIG"
BASE COUNT         8822 a         5225 c         2818 g        10988 t
ORIGIN      
        1 aaaattaaat ataaaaatca actattattt tttaaatagc atctttctta atctattatt
       61 gtaataacct ataaaagaac tgtaaacata gtcatatact ataaaggcta tctcaaatga
      121 acctataata aatatgttta ttggaatata aactattgtt cttaataaaa aatacaatat
      181 aagtatcatt atatttgcaa atactaattt cataaaatac tcaatatata tagacctatc
      241 tttttctata agatatttta caataccata tattccaaag gtaagtgaat atacaatcca
      301 ttgagactta cttgccatca ctataaatcc taaaactata gaacctatat aaaaagcaat
      361 tccactctta aagccccatt ccattattac aattgatact aaaagtgatg ctaaccccat
      421 aataaacaat gtatttacag gaataatatt tgtaagagtc aacaatatca catttaatgc
      481 taataaaact cctccataag caacttttat actcatattt atcatcctaa caacaagtta
      541 ttaaatctcc accaaaacat tcacaacatg aatctaaaca ccaaagctta gcacaatttt
      601 cacaacaatc gtcacctaga caacaacagc aatcatcaca gcaacagcaa caacatccat
      661 ctaaatcatt acgtcttctt ctattataat tgtcagccct attgctataa tcatttctat
      721 attgtgtata acttctaagt gcatcactat attcactatt ttcaggctcc ataaattttg
      781 ctctttttaa gaagtcttca ccttcttcat aatatcctat gttcattgca gaaaagccag
      841 ttaaataata ccactctgca catttgtcag tcaaagtttt taaaaaatca tatgcttttt
      901 taaattcact attttctatg aatgctcttg ctctcataaa attttcatta ttataagtat
      961 tactgtacat tctttccacc tctactttct aatatattaa tatatcttaa ataaacacca
     1021 gaataaatta tattttctat tattccaaca tttttattta gatttagatt atcaattccc
     1081 ctagctaaaa accctaatga tgttactatt atttttttta ctttttcttt taactcatca
     1141 tttttatcaa tatactctat aaatggatta tatcttcctt tcttaaaatc ttcatctaaa
     1201 tcttcatagg catctagtag atatatatac ttacctatat taaaacctat cattcttaag
     1261 tttctctcat tttcatcttt tttatagaca aatacttcac ccattatatt tccaaatata
     1321 ctagatactt catctatatt tatattttta tctttttcta acttatttaa ttcatttagt
     1381 tgttgtttta tatactctgc tttttcagga tacttttcat atgctaattt taatttgttt
     1441 ttatatatat aatatgccaa tttatctttt atgcgtttat catccattaa attatcttcc
     1501 aatttataat acgttaaaag aatattcata cttgctgcat attctgtaat atcatttgta
     1561 attacctttt tctttttaaa tggatttact atacacactt cctctgtact tatagactct
     1621 gggttatata ctgctgttaa taaaacaatt aaaaatgtta tatcataatt caaagaaaac
     1681 ctagagattt ctccatgatt tctttttaaa tatttacata gtccacaata gtaagctttg
     1741 taataatcgt attctctaaa tgttaaatcc attttgttta ttttaacata tccaaacata
     1801 aatactcctt tttgtactat tttttattta gattatatat agtataacac tttttatcaa
     1861 gattatataa tttacagtat tctaagaaac ttaaaaaatt ccttctaatc attgtatctt
     1921 aaatttaaaa taattagaag gttgggttaa aaataatatt aaattatata cttatattaa
     1981 ctcatacaag ttagttgtgc ttatatcaat ttaagtttta aataattggt atatatttaa
     2041 aactacctac agtatccttc aattcttttt ttgctttatt tataaccaat tcatcaagca
     2101 ttacatcatt tattgaacat gatatagatt tagatgccaa taacattgct ttaaatccca
     2161 tatcagaacc tgcactggca caagattgcc atgtatgggc agaaatacca ataggccaaa
     2221 ctaaacaaga acactgtgct gtaggaatta tgtaactaac atctccaata tccaatgata
     2281 atgatataca gttattatga gtaaatgtat catccgtaac ttcatcatgc agattcatct
     2341 tagtaatatc cttatttcca cttaatacac tagaaacact acttctttta tctatagaca
     2401 ctgtctcagc taatttatct gcaaaatagt agtcatcttc attatagttt tgtacaccta
     2461 ctaatttcat attttgtgat agaatatctg ttaatattgt atttggaata tagtcatata
     2521 ctccacttat aatgtcatat tccataatag taccagtcat taatgctgct ccttttgcta
     2581 tatcaacaat cctaccaaat acatctctta catgctccat tttaactcct cttatgtaat
     2641 accacacttc tgcaaaatca ggaactatat ttggcatttt tccaccatta gtagttacat
     2701 agtgtactct acaattatca aatatatgtt ctctcaaata attacaacct atattcataa
     2761 gttctactgc atcaagtgca cttcttccgt tataaggagc ttgtgctgca tgggcactaa
     2821 tcccattata tctaaatttc acagagtaat tagcaagtgt tgggattctt ataggagtat
     2881 ttatatcaaa tggatgccaa ctaaaagcac aatcaattcc atcaaaaaaa ccttttttta
     2941 tcatcaatac ttttccagat aaatcttcct ctgctggaca tccatagtat tttatagtcc
     3001 ctttcagctt ttttagtttg attaattcct ttactgcaat tacagctcct attgaacctg
     3061 tacccagtaa gttatgtcca catccatgac ctgcaccacc atctacaata ggttctttaa
     3121 tagtggatac tttttgagac aaactatcta aagcatcata ttctcccaaa actgctataa
     3181 ctggtctacc ttctccatag ctagccgcaa atgctgtatc cataccttct aattcatcta
     3241 tactaaaacc atattttttt aagtattctt tttgtaaatt tgaagcataa tattctttat
     3301 actgaagttc tggatttttc catatcttat ctgatatatc tcttaattct ccctgtatct
     3361 catctatata ttccatcaaa aactttttat ttaaattctc catattaatt atataaacct
     3421 ccctgtttct aataacttat cgattatcag ccaaaatgac taattttaac tgatttaaaa
     3481 atattttcta tcatacaacc attttataca ataaaacctc ttgacttgta agtttcattt
     3541 tatcaagtaa gaggttttca agttttataa aatttttagc atgttaacca atcaagcata
     3601 ttaacccata atttattata gtatttccaa tcagtaaatt cttttggacc ccagtgaggt
     3661 gcacagtcag aactaaagat agcagatttt ccttttccaa actcgccaac agctacaaat
     3721 gggtctcctc caatagttgc taaaacagga caattatctc tagctactgt tttgttatat
     3781 cctaaaaact ttggccattc tgttggtata ccttttaata ctggatgttt agagtcaata
     3841 acttctggaa ttattcctgc tggtttttca actctatcat ctttatctat cattgttatt
     3901 ggtaatacat ctttgatagc agtttctccg aatctagctt ttgcatctat tcctgtaaat
     3961 gacatatatc cacctaccat gattaacgcc ccaccattat ttacatactc tttgattagt
     4021 tcaagtctat ctggattact attgcaatct ataaaagtac tattagataa taagaacgta
     4081 ttagaaccta tatcagataa tattatacag tcatactcat ttaactcttc taatttatac
     4141 ggaaaactat ctgctgctac atgtgcaggc atatgaactg tctcatatcc tccagattct
     4201 attgcttctt tcaaccactt tacagcttct tcataatgag ttgtaacaaa cgtatcaaat
     4261 ccttttatat gagttatatt ttttacccaa gattccccta ctattaatac ttttttcatt
     4321 tatatattcc tcctactttt ataagtttaa tattagactt gcatacataa gtatactatc
     4381 taaatactcc ttaatttcaa catattcatc aattgaatga cattgttcta ttgaacctgg
     4441 ccctaatata agtgttggta tttcagctaa atttttcatt aatctcgcat cattgccagc
     4501 tgttgaacct tttatctcta attttttccc tagagtttct tctagtattt tatgtgcatt
     4561 cactacaaaa tcagaatcct tgtccatctc aaaacctaac cctgattgat atatgtttat
     4621 atttggttta ttatctttaa gccataaatc tccattagcc cttgtcatta tgacatttgt
     4681 tacatcattt acaacttgtt cataactcat tatatttggc aagaagtgta cacataaatt
     4741 aaatacacac ttatctggca cagtagaacc tgctgttcca ccatttatta cacctaaatt
     4801 tattgtaggt gaaggtaata gtggatgttt atatatcata agccagttat gttccaactc
     4861 ttttatatct tgaagcaaca acatagcctt ctcaattgca tttacaccct cccacttact
     4921 accacagtga agagacacac ctttaacctc aacttcaaag aatacaaatc ccatatgtgc
     4981 aactattaag ttttgttctg atggttcaca aattatgcaa caatctccat caataccatt
     5041 cataactgca tttatggtac catttccgcc accttcttca tctactactg acattatttt
     5101 tacattacca ggtacattta gaccactatc ttttataagc tttacagcta atattgatgc
     5161 tataagtcca gatttcatat cagcagttcc aagtccataa agttttccat tatcttctgt
     5221 agcacgatat ggattatatt tccacttact tatatcacct ggtggcatag tgtctacatg
     5281 accattaaat acaattgtct taccaggtaa atcatcacta tacttacaaa ttaaattata
     5341 tctatcttga taattgtgac ctagatttcc ttctttatat atattttttg cttttttaat
     5401 taattcttcg ctcatttctt gtctatctac actaaaacct atgtaatttg caagtttttc
     5461 aatatattcc tgtccttctt tttcaaatcc acctagtatt ccatgaccaa catcttcagt
     5521 ctttatactt acaagttctt ttaaataatc tatgtattct tgttttttac tatttaatgt
     5581 atctaagaga atttttttca tatttcctcc taagatttat ttttacattt aaaatttcaa
     5641 gtctaatctc atctatttaa gccatagtaa actttttcac ttagcatatt cccattcaat
     5701 gtataaagat gtatgcaaat taaagtataa aaatatatac aaaaacttaa taatttttat
     5761 agtttataag ctaaaattta attactattt agatttagct gattttttta attttatatt
     5821 atttatccat atagcaaata acattaccaa accttttata atcatttgat tatatgaaga
     5881 tacacttaat aagtttaatc catttcctaa tattcctatt attccgacac ctatacatgt
     5941 acctactata gaaccagaac cacccatcat attagtacca ccaagcacaa cggctgctat
     6001 agcatccaat tctgctccat ctccaacagt tggtgaacct gatgccaaac gtgatgatag
     6061 tattatccca gctaatgctg ttagtagtcc agctagtgcg tatactttta ctataacttt
     6121 atttactttt actccacata atctagcggc ctcttcattt ccacctactg catgtacatg
     6181 acgtccaaaa ttagttcttt ttaatagaaa atgtcctgct attacaacta taatcataat
     6241 tataactggt attggtattg gtccaatata tcctctaccg agcatcttaa atgcatctgt
     6301 atttactgat ataggtcttc ctcctgtaaa tacaaagctt gccccactta ttatcgttaa
     6361 catggctaaa gttacaatga atggaggtag tttttgtgtt gaaataattg caccttttat
     6421 gactcctaaa attgtcccta ttgctaatgc aagtatcata cctacccata taggtgcatt
     6481 ataattcact actgttgtag cccaaaatgt acctactaaa gcaattactg ctcctacact
     6541 taaatctatt cctcctgtaa ttattacaaa tgtcatacct actgcaagta ttgcatttat
     6601 tgatatttgt cttccaacat taattaaatt atctaaagtt aaaaagttag atgaagctac
     6661 actcattatt ataaaaatta ttacaagtcc tacagctgca ctcccttggt ctctaaatac
     6721 acttgttaat tttgtcaata atggagaaga tttttcttct ttatgcttag ttattgaact
     6781 cattaatttt acctccgaaa gccaatttta atacattatt ttcaaccatt tcttcacctt
     6841 ctaaaaatcc acttatatga ccttctctca taaccattat tctgtcactc attgatatta
     6901 attctggtat ttcagaagat atcattatta tacttacacc actctccact aaatctccca
     6961 ttagattgta tatctctgcc ttagcattta catctatacc tctggttggt tcatccataa
     7021 tcaatatatc tggttgtaat aaaagccatc ttgctattat agctttttgt tggtttccac
     7081 cacttaaact ttgtataact tgattcatat gtggtgtttt tatctttaaa gcttctatgt
     7141 atttttctaa aatctctttt tccttttttc tatctataat tgatttaaac ttagatatct
     7201 tttttaaccc agccatagtt gtattttctt tgacactcat ttgtaaaaac aatccagtac
     7261 cttttctatc ttctgttatt aatcctagtc cattttctat tgcatctgat ggagttttaa
     7321 actttacttc ttcattgttt aaatatattt ttccagaatc atatgttcta agtccaaata
     7381 tagcttctac tatttcactt ctcttacttc caactagtcc acctattccc aatatttcac
     7441 cttttcttat attaaagcta atatcattaa aaacattgtt cttagttaga ttttccactc
     7501 gcataatttc ttcttttatt tctacttttc tttttgggaa aaattggtct agctttctat
     7561 ctatcattaa attaactaat tcttcctcag acgatacatc tttggtattt aaagtcgaaa
     7621 tcgttttccc atctcgcatt atagttattc tgtcacaaat ctcaaagagt tcttccatcc
     7681 tatgggatat atatattata ctaactccac tctcttttaa atcatttata agtctaaaaa
     7741 gtatttcagt ctctttttta cttattgaag aagtaggttc atccattatt aatactttag
     7801 tttctgatga taaaactctt gctatttcta ccatttgttt ttgagctata ctaagctctt
     7861 tgactaaact atttggattt atatctattc ccatattatc gaaaatcttt ttagcttctt
     7921 cattcatttt tttgtcattg gttaagactt tgccatttat ttcttttccc ataaaaatat
     7981 tttgtgctac tgttaattcg tcaaaaactg ttagttcttg atatatggca tttataccta
     8041 tctttttaga gatatctggg gatatattct ctatttcttt gccttcaaaa ataaattttc
     8101 cagaagtttt cgaatgtgcc ccagttaaaa tctttatcat tgttgattta ccagcaccat
     8161 tttcacccag tagtgcatga acttcgccat gaaataactc aaaattaaca ttatctaatg
     8221 ctctgacacc aggaaattct ttagcaatat tacttaactt cagaattata ttactcataa
     8281 tgacactcct ttttaaatag gatttctttc aaataatctc attttatttt actaattctt
     8341 aaagtctttt atattttcct tatcataaag taaagctggt actggtatat tcttttctac
     8401 cttttcccct tttatagctt ttactactgc ttctacagct aaacgtccca tctctttagg
     8461 ttgttgttga atcatagcaa gaatagttcc atctgttatt ccacttgatg cttcttcagt
     8521 taagtcaaat cctacaatct taacatcctt attttttgca gattcaagtg cagctttagc
     8581 tcctagtaca ctattttcag ctgttgcata tattatatct acatctggat tagattgaat
     8641 taagttttct actgtattaa gagattcttc tctgtcatat cctggttgtg aatttaatat
     8701 cttaacattt gctgaacctt ttaatgcatc tttaaatcca tctattctct gcatttgtat
     8761 ttgagatttt aaatctgtca ctattgctat ttctgacttt ccacctaact tttcatctat
     8821 gtatttttta gtgtattccc ctaatttttt accagcatca taactaactg ttcctacata
     8881 acttgtaata ttttcagtat ttaatacagc atctactgta actactggca ttttttcaaa
     8941 tgctccttct actgctggta ctatagccga attatcagtt ccaagtacta tcattccatc
     9001 tacattctgt tgtataaaat cttctattgc tgatgtttgc ttagctacat cttggtctgg
     9061 gtcatttact ataactttca cacctaattc tttagctttt tcttttattc cctcatcaat
     9121 atctataaaa aattgatgtt gaagagttat tgtagatact ccaatagtta aatctttctt
     9181 gtctgaacca cctttattat ctgatttact tggtccttcc attgaacacc ctgttaacat
     9241 tcctattgct agtattccag caattcctaa agataaaatt tttctcactc tcataattaa
     9301 cctccatttt taatagtttt aaatttgaac aaattagatt tttatttatt atttaaatac
     9361 ttttcaacat cttctttata gggcatacca gattgtgcac ctagtttagt tacagaaaga
     9421 cctgatacta ctactgcata ttctactgct gtatataaat ttttcccttc tgttatagcc
     9481 actgccaatg ctccattaaa tgtatctcct gctcctgtgg tatcttgaac atcaacactt
     9541 atacaagata catgcttgat ttcctcacca tcataaaatc tagctccatt ttttccttct
     9601 gttattacaa gtttatttgg atattttttt aatacttctt ctatcccttc atttgtgtca
     9661 aaaactatct tatattcatg ttcatttggt gttaagtaag ttactttttc tattatatct
     9721 tcatttagtt ttactgctgg tgctggattt aataaaaccc taatcctatt ttcaaaacag
     9781 aaattcacta cataatttat tgtttttaat ggaatctcta attgtagcaa tactatatct
     9841 gcattcttta tctcttcttc attcttttta attaattcta tatctactag ctcattagtc
     9901 cctggtacaa caacaatact attatcattt tcacataatg ttataacagc aacaccagaa
     9961 cttttgtgat tatttctgga tactaagcta taatcaactt tattcgttct aaaatttctt
    10021 attaactcct ctcctaagcc atcttcccct atgcatgata tcatttttac atttgctcct
    10081 agtttagaag ctgagatagc ttgattagcc ccttttcctc caggtgatgt aaaaaaacta
    10141 ttacctaata ctgtttctcc tttttctggt cttttatcta cagaacatac catgtccatg
    10201 tttacacttc ctatgacaac tatatttccc attacattac tcctttacat atctatttat
    10261 gtctctcgtg ctgttattca ctttcttttg ttgaatctct tatgaccaat tctggtttga
    10321 aaatcaattc atcaaacgat tcttttttat ctcctcttaa acaatttatc aatatttcac
    10381 tggctttata tcctatatca taagacggct gttttactgt ggtaagagaa gggcttatta
    10441 gacttgatgt ttgaatatca tcaaatccca cgacacttat atcatttggt atacttaatc
    10501 ctctttcttt aagacctctt atgacaccta ttgcaatcaa atcatttgca caaaatattg
    10561 catcaaaatc tatcttttct aaatttttta taaattcttg tccccattca atcttatatt
    10621 ttccttcaaa tacataagca ggattgtagc ttaaattaca ctcgattaga gcctttttat
    10681 atccttctaa tctatctact gctatttcat tttgaagtgg accacttagg tatattattt
    10741 tcttatttcc aatatctaat aaatgttttg tagctatata ggccccttca aagttattta
    10801 ttttaacaat tccttttaag gtttctttgt tcatacttat cttcttatca atcaagacag
    10861 ttggtatttt tatatcttta taatttgctt ctttccagtt tgtatttgaa gatgctgcaa
    10921 atataattcc atctatcatc ctctctgata gtgttcttat tccttcatat tcttttttag
    10981 catcttcatc tgtattacat aaaattatat tgtaaccttc ttcattagct ttgtcttcta
    11041 cacctcttga tacatctgta aagaatggat ttcgtatatc tggtataaga agccctatag
    11101 tattagtttt tttagttact aaactttgtg ctaatttatt tggtacataa tttttttcct
    11161 tcattatttt caaaattttt tctcttgttg cctgacttat attttcatct ttattattaa
    11221 tgacttttga cacagttgtt ttagaaacac cagccaattc gccaatttct tttatagtta
    11281 tactcaagaa gttcaccccc aaaatttagg aaaccgtttt acttaaccgg ttacctagat
    11341 actatcacat cttttttcca tttgcaacca ttttatataa attttgatat ttttcccttt
    11401 tttcaaaata ttgcaactta taattcaatt ttaatattta aattaatctg caaaaaaaaa
    11461 ttaatatact tttataatta tctaaaagta tattaattaa tttaagcagt atttattaaa
    11521 gttcaaatcc ttgctctata atcatttttt tatcattttc aatcccactt ccaacagttg
    11581 tcaaataatc accaacaatt gcagaattta ctcccccatt aaatccaact ttgtcatatc
    11641 cctttaatga tagccttcca ccagcatacc ttatgtacac attaggaatt ataaacctaa
    11701 atactgctgt tgtttttagt atttccattg gttctaatac atcataattt tccattggtg
    11761 tattcttaat aggatttaaa atgtttattg gaaatgattt tacatttaat tctcgtattt
    11821 caaaagccat ttttagtctc tgttcttcac tctcattcat tccaattata ccacctacac
    11881 atacatctag cccagctttt ttagcatctt ttatagtttt tatcctatct ttatatgtgt
    11941 gtgttgtaca tatattatga aaattatctc tacacgtctc aacattgtgg tgatatgtag
    12001 tcacacctgc tgacttcaac attttagctt gctcataatc tataatacct aaagatgcac
    12061 ataattttaa attagtattt tccctcaatc cttcatagat atttaaaata ttattaaact
    12121 cctttcctga cataccttta ccacttgtaa ccaatgagaa tctatgcact cctttactct
    12181 ccatctcttt agctctattt aagatttcat tgtaatctaa taatgaatat tcttctacac
    12241 cagttttata atgagatgat tgagcacaaa atttacagtc ttcactacat ttgccagatt
    12301 tagcattcat tatcgtacat aaatctgctt ttcttcccat aaaatattct cttatttcat
    12361 ttgcagattt taatagaata tcaaaatcgt ttttattatt tgtatcaagg cttattaaat
    12421 taagagcttc ttcataactt atttcttttt ctctcaatac tttatttttt aattttatta
    12481 tgtatttttc catatatcct cctatttgtc aacttaatat ttttatgtag tttacttatt
    12541 atatatagta ttgcatatat aacatttatt ggtcaacttt attttagaca caagtttact
    12601 atacacaaaa aaatactgct tcaaaataat attagtaaat ggattataca tattattatt
    12661 aaaacagtat tttttattag aatataattc ttactggttt ataaaactat ccttgtctta
    12721 actccatcca agctttccat tgtaataacc tctaagcctt catattctct aaatagccat
    12781 tttaaatcct tcatggcttg attaacctga ctttttgtta gattatttat aaaatattct
    12841 tttctagttt cctcatactc tttgctagtc aaaaagtttc ttagaaaatt aaacgcaaat
    12901 acacttggaa tattgtttac attagccttc ttgctgtttt ccttctcata gtaaattttc
    12961 ataaatgcat actcctttga tacttttaga tttctcttac aataattata tgcatttaag
    13021 tgctactatt cttataattt tattaattca tattctttac ttcccatacc catttcacat
    13081 gcatagtcta ttattctagt tccatttaca tgaggatgtt catgtttgaa aacatcatga
    13141 cccatttcct tacaaatcaa atcatagcat gcttgttcaa tagctacagg gtctgttgat
    13201 gctaatattc ctatatcatg ggcaattggt ctacctgacc aaggtacaca atcacaaagt
    13261 ggagttacat tcataacaaa agttatatac cctaccttgt cttttttatt tgatactgct
    13321 ccataagcgt attctgccat cttttcgaca aacacatctg agtcactttc ccattgtata
    13381 gttactgctc tagttggaca gacagttgga cattctccac atccataaca tacatcagaa
    13441 tctataactg cttttttatc cactatactt atagcttttg taggacaaga attaacacac
    13501 tttccacaac ctacacactt cttttccttt actactggag taacatcaga atgttgcatt
    13561 tgttttcctg ctgcacttgc acatcccata gctaagtttt ttaatgctcc tccaaaacct
    13621 gctgcttcat gacctttaaa atgactcatt actatcattg cagaagaatt ataaatttct
    13681 ccacctattt ttacactttc aaaatgtttt ttgtctattt ttacattctc ataatttcta
    13741 ctatataatc catctgcaat tattactggt gcatttacaa ctgcataagc aaatccattt
    13801 tctatagcag ttgtaagatg gtctacagaa tttgttctac tccctgtata cagtgtattt
    13861 gtatctgtta aaaatggctt tccttcgcag tctttaacct tatcaaccac ttgtctaact
    13921 gccacaggac tcatatatgt agtatttccc ttttctccaa agtgaagttt aattgcaact
    13981 tggtcattct tttctattaa atctttgaat cctgcttcat caaataatct tcttacattg
    14041 tttggtacgt ttttattttg cgaatttgag tataaatcac aaaaatatac ttttgacttt
    14101 tccatatcta caccccttta aaatatttac ttatactaat tttatcactt ttaaagtaat
    14161 ttttagtata ttttaacata attaaatatt cttatattta tttgatacaa ataatattat
    14221 ttgtattatt ttctacactt aattctatat attaaaaatc aaaaagactt gaaacttaag
    14281 tccaagtctt tttgattttt aggtgtagtt tttaacaaca taagattact ttaccttgtg
    14341 ataaaggtat tattaaactg gttttttatt aaataaaata acagctgcta gtaaacatac
    14401 tacagaaata agaactgtta taataattgg aataataaat tctggcattg ccactttccc
    14461 tgaaatcaag tctatatttt ttgacatcaa gaaattagga gtataaggtg caatctgttt
    14521 aggaatagaa attaaactca ataaaagagt tgccaccaat acaagtaaaa cactggcaaa
    14581 tgctggtcta aatagtacac acccaagaat taatacacta atatacataa ttcctataag
    14641 ccataaatta aatgctgaaa atataatatg attaagattt gcagttggcc aaaaatatat
    14701 tgtatagcca taagcaaata aaaaactcaa ccaaaaacca attgtcataa taatcaccgt
    14761 aacagagaat ttagacaata ctatagatga acgtgataaa cctttagtca gcataataac
    14821 aagtgtccct ttagaatatt catttgataa aatattacaa aaaataatta gagttaaact
    14881 cataccaaga ccagagatat tgctaaaaaa ttgtgtccaa gaatctaaag cagttggtgc
    14941 agcagtaact tttaaagtcg gtgcaaaatg agcaatcaag tctggcataa attttgcaga
    15001 aataggacta cttataccaa atattagaaa taaaataatt aaactaaata atttataatt
    15061 tcttaaattc tctttaaatt ctttttttgt aaatgctatg tattctctca ctttactacc
    15121 tccaaaaata aattttcaag tgataattct attagttcca tctttactgg acaaagattc
    15181 atttctgcga gtgtagatat aactgttttt tgtattgctt ttatatcttt accatgcaaa
    15241 acaatttcca tattagtttc ttctgaatct tttagtaagg atttaatacc atcactagac
    15301 ttaaactttt gaatttcatc attacttgca aactccagta aaagtctatc ctttccgtgc
    15361 atacttttta tttctgaaag tgtaccacta agggcaatgc ttcctttgtt taatatagca
    15421 acatggtcac agattctttc aacatctgat agtatatgtg ttgaaaatat tacagttgtt
    15481 gagtctttga ttttaagcat aatatctaat atttcttttc gaccaacagg gtcaagtgca
    15541 cttgttggtt catcacaaat aagtaatttg gggcgtgaaa gcaaagcttg agctatacca
    15601 agtcgttgct tcattccacg agaaaaccca cctattcgtc tcttttcatt tccaagaccc
    15661 acaagtgata acaactcttc actccttatc tgtatttctt tttttgaaag tcccgtaatt
    15721 tcaccacaaa gagaaagata ttcaagtggt ctcatatagt tgtaaaactc tggaacatca
    15781 ggcagataac ctacatgacg attagaactt gttttcccat aggtcacttt ctcaccacaa
    15841 acatctatag ttccactatc tggtttaagt aacccaagta ccattttcat tgttgtagtc
    15901 ttacctgcac cattcttacc tacaaaccca aagactgacc cttctggtac agtaaaattt
    15961 aaattatcta ttatctttcg attaccaaaa ccttttgata atccatttat tgagacaata
    16021 ttcatcattc ttcacctctt cctattgtaa aatacaaaat tggtcctatt agactaataa
    16081 acagtactac aataatccat attgccttat ttccaatttt ataatttgga tgtctcaata
    16141 catgaacaag tgcagtaatt ataagtatta aatctaaaat aataacagga accaaaagtg
    16201 gtaagtattc catcaaattt tccataatct tattcctcca ataaagataa tttttctata
    16261 agaaatttgt attctttgtt ttcacttaat acagaaaaca ttggattatt tttaatagca
    16321 gcaacaatac ctgcttttac gattgtatca tcacgtgtaa tacctgttcc aatttcagaa
    16381 atccatctgt caagtttatt aaaaaagtca tccccatgta ttataattgg gtatatatca
    16441 cgtgttgcta aatctacata ttttcgtaat gatttaatag ctccttcatt atcaccatgt
    16501 attaaattaa tttgtgcctg tgttaaatat gaagaaagca taatagcagg agataatgta
    16561 tcaagctcaa atatatctga aattgctaga atacgtcttt ctatttcctt catttgtaat
    16621 ttatctgcat tttgcaaaat acccactagg ttttggacta atgatataag gttctgatat
    16681 gcaccaagtt gaaatgtttc tctcgcttca tccatttgac cattcatcat ttgcccctgt
    16741 gcaagaagta tagattcatt tatcatggga aaattgctgt tttgcaataa atcaatcact
    16801 tgtatgggtt gatttaacaa tatataacaa gttgcctcca tactttttgc ttgacggcaa
    16861 atatctatgt cattacaagt ttcctgtata cgaataaata tttctaatgc ttcactaatt
    16921 aatagttcac gctttttttc atctacaata tcatagtgat taactattaa aagacccatc
    16981 cgaaaaatca aaggaaaaca tgaataatat ttttttatga tttctcttat ttcatccatg
    17041 acttcatcaa atggtttaac cgtaaagtct ttgcacagtt tattatacag tttattaata
    17101 tcttccttca tcatttgagg ttcataacat attaactcat caacggtaat gttgaaatat
    17161 gtcgcgagtt gcggtaaaaa agttatgtca ggatagcttt gtccagtttc ccatttagat
    17221 acagaagctt tagaaacgcc tatataattt gcaagttcat cttgtgtaat tcctttttct
    17281 ttacgcttta aaatcagcgt ttttgcgatg tttaattcat tcatgtcaat ccctccttat
    17341 gtataattat atccttaaac atgtaaagct aacaagcgat ttgaagttga cttttgacga
    17401 aaaaatcaac cacagagaaa caaataaaca agtatataaa atatatattt aaatttatct
    17461 ttaatctctt ttgtaaaata tgtaaacttt gtaaataaaa tccatattat ataaaactaa
    17521 tagttaatct atctaaatac agtttttatt tatcacgttt ttaaatattt tgtgtaaaac
    17581 ctcttttaat ataaacacct tattttcatt tctcatttca tattcttttt aaaatactga
    17641 atataactct tcgattttcc atttaattct tttataaagt tattattcgt atttatttct
    17701 ctaattataa caggcaacta tcatgtattg gtatttgtct attatataaa aatctataaa
    17761 agaaaagcag tcaatcattt agattaactg cttgtacata tatcttatta aactgtataa
    17821 actataattt ttattattct ccattttcat cccaagtcat aaatccccat ccactatctg
    17881 gataagtatc aatagacttt tctctaatca tataaccaac tttataacac tcttttgtta
    17941 ttttattaga tagtatacat ccttctccat ttatctaatt ccattcaatt aacttttctt
    18001 ttcaacctta acttttataa aatcatcttt tttaataatt tcatacttat tttctcttca
    18061 acatcatcaa atgagggtgt ctcaaaatta actttttact tcataaggca ctcttttttg
    18121 tatgaagtaa aatatatttt catcatttca acaataagta agaggcttat ctctattttg
    18181 agacagcccc atttgatggt gttgaacatt taaataaggt tgaactcgtt ttatctctct
    18241 ttttaagata tctgtaaatt tgcacataaa gtctcttctt cttttacaat tctaatagtt
    18301 ttattaaagc caaagtatat atctccatat tctttcgtat atgatttatt ggtactcttt
    18361 catctactcc atgaccacca gttttatacg ctaagaactt aggtccaaac gccaccatat
    18421 ctttaaacca acttgcatat gtgccaccac ccatagcaat tgtatatcca ttttcatctg
    18481 ttacttcttc ataggcttct aataattttt taagtactaa attactctca tccacataat
    18541 gtaagtcttt atgagcaata acctctattt tccaaccatt acaaaaaacc tttttgaatc
    18601 tatctattat attgtttaaa actatatttt taccatacct tatatctaac tcacatacga
    18661 ttttttcctc atttactttt aatattccta aatttaaggt agtgtttttg aatacctcat
    18721 catattgatt aatccctaat gcatctccat aataatcact acaaaggtat tttgaaacta
    18781 attcgatatc ttctctatgc tgaaaatcca tattatcctc aactatcttt attaccattt
    18841 tagatatagc attttctccc ttttcaggat tacttgcatg tacagctact cctttagtag
    18901 ttaactctga aataattcca ttaataattt ttgcagtaca atgagatgga acaacatttt
    18961 ctttagttcc tccccttact attatgtaag gtttactttt gtcaatttta atttcttttt
    19021 ctatccttat atgaaccctt cctttttctc cattaaccac tggatacata gcatctggag
    19081 taaatcccat acttggaatt tcttcacctt tatctaagta atatttgata catttcatac
    19141 ctgtctcctc atttgtacca aaaattaatc ttattctttt attaattgta ggatacatat
    19201 cctctatgtt tttcaataca tgtataagca tagatacagc acccttatta tctattacac
    19261 ctcttccata aataatattg tcaattactg ctcccgaaaa tgggtctaca ctccacccct
    19321 ctcctggtgg aactatatct aaatgcattg gaatccctat taattcctct ccctctccaa
    19381 tttctgcata tccaatataa ttatcaatat ttttaacact aaaacctaaa ctagcacagt
    19441 aatctaaagt tttatctaaa gattttttag gtcctatacc aaaaggacat tgtgaaatag
    19501 gttcaccttg ttgtgaatca atttttatca attcttttag aaattctatg gttctattta
    19561 aactattatc tacacttctg cttatatcca taaatcaacc tactttctat ctttttaaag
    19621 cctgtcctta aagaacaggc ttttagcatt aaaaattatt tttttgtaaa tactaattca
    19681 caaacttcat catcatgagc acatttttga gcaaaatcta atttaacacc ttgtgcgcag
    19741 tcaactcttc cataatcccc ataacaagct aatctacaaa tttcttttct ttcttcttta
    19801 gttaatccat attctttcca agcagcatct aatggacagt gatggaattt taaaacgcca
    19861 ttctcatctg tattttctac taattccatt tcaaacactt tttgtccttt actgttatat
    19921 aacatttctg ccatttttcc tggagtatct gctacgttat atttttgacc tctcatttta
    19981 ccaaatccat atattgcttc tttacatata tcatctggat tagctcctaa tttttcagct
    20041 tctttcataa gaagatagaa ccatactgct ctttctcctg ttgctcctct aattccttct
    20101 aaatattcct tttctgtata cattttttct gacatttctt tctcctccta aaattaaatt
    20161 ctatatttta atcactgtga attagaatat tccaatgaat gaccctacca atgctaatac
    20221 aactaaaatt actaataact tagttattgt ccacttcttg tatttcattc cccaatatac
    20281 gaagaataca acaccaagtg gtaataaacc tggagcaatc atatttaatg tatcttgaac
    20341 aacaattgct ttaccaccaa tatcaaatga tagcggagtt gaaagcttaa ctgtagttgc
    20401 agataatgct cccatcataa acattcctag aattgaagca ccatcaatta tcttattgac
    20461 aataccacct gatagaattt tcttaattga atctctacct aaagaatatc ctaaatgaaa
    20521 taagtaataa ccttcgcaaa atagtaatat actaaacatt attgggaaaa atgcacctat
    20581 aacacttcct tctgctccaa atgaagcacc taaagctaag aatattgttt gaaatgtacc
    20641 aaaatctaaa gtatctccaa ttccagccaa aggtcccata agaccattct taacacctga
    20701 aataacttct cctgggattt ttccttcagt agctttctct tcttccatag ctaaaactgt
    20761 accgtgaatt aatcctcccc atatagcttg agtattaaaa aattgtaagt gtctctttaa
    20821 cgcatctact aaatcttctt ttctcttata taacttttct aaagccggag tgaaacttgc
    20881 acaaactgct aatgcttgca ttctttcaaa agagttggat aattctgctg tccaccacca
    20941 tcttagatat gtttttgtta catctttctt tgtaagtact cttgtcttat taacttcagt
    21001 tggaccttgt ccatttttaa cttctttatt ttttatttct tgcattatac ttcacctacc
    21061 cttttgtctt ctttcataaa ggtaattaaa agagctatac aaacaccaaa tattgcagct
    21121 gcagtaattg atatttcaaa atattgaacc ataaaaaatc ctataataaa cattggtaaa
    21181 aatttatttt tacctatagt aaatattgtt gttgcaaaac ctaatgcagg taatataccg
    21241 cccattactg ttaatccatt cattaaccat tgtggtataa catctaataa tgattgaact
    21301 aaatctgctc caaaaaagtt acatacaaaa actattggaa accttaaaac aaacccaaat
    21361 attaaagaat aaagtgttgc acatctccag atgccttttg tattaccttc ttcagcatac
    21421 ttatctgcct tatgaaccaa tacagcatta ccagttcttc ttaagttatt aactaatact
    21481 cctataacac ctaaaggtac cgcaatagat actgcaactt ctgcattaac accagtttga
    21541 agtgctactg gtattgcaat aagagcagct agacatttat ctgatggaat atttccccct
    21601 gctgcgacca ttcctagata aaccatttct atacttgcac caacaatcat accagttgtt
    21661 acatcacccc aaagtaatcc aaggctaaaa cctataaata aaggagactt tagtgttgag
    21721 aaaagggtat accctgcaag acctgctgca aaccaatacc ataatcctgt tacaattgcc
    21781 aacatcaaaa cagattccat ttataattcc tcctaattta tattttttat ttaaatttct
    21841 ttaacgcatt ttcatatgtc atagatgatt cctctggtat aacttgtagg actatttcaa
    21901 ccccattttc attaagttca ttaagttgtt ctacatcctc atttcctaat gaaacagctg
    21961 taaacaccat tttctttcct gcttcatttg gaatacctcc taattgaatc ttttcatatt
    22021 taagtccagc tttataagcc ttatttgcca tatcaacatt tttaaataga ataaatatat
    22081 ttttatctcc taaagtgttg ttttggaatg cttgtcccgc atcttctgct gttaaaataa
    22141 ctacttctac tcctgatggt gctgccattt tatatatgtc agccataaat tcatcttgac
    22201 ctaagaagtc atcaactatt attatttttt gagcttgagc gatttttacc cattttgtta
    22261 taacttgccc atgaattaat ctgtggtcta ctcttactaa tgttattttt gccattatat
    22321 tcacctcata atttatattt ttttaaattc tttaattaaa tccacacaaa ggttattatt
    22381 ttttttgacg attctttctg gtaatgtatg taatttatct tctttgctta attcttcatc
    22441 tacgattaaa attgtctgta agctaagtcc atttattgca tctattcctc ttcttatagc
    22501 aacagctatt gcaacacttg atgtagatcc tccatataaa tctgatatta taattttcac
    22561 atcttcttta tctactactt cttcaacctc tttcataaat acagaaacat ctttttcaga
    22621 acttagtgat aaaacttcat attgatttga caatccaaag tttttatttg tttcttctac
    22681 taagactttt ccccaagttc catggcacag tatgattacc ttatatttat tcattgctaa
    22741 ataaactctc ctttatagcg attaatattt tggtttaatt ctacttcttc ttcatcaggt
    22801 accatttgaa ttgatacatt aatattcttt ttaataaatt catctattaa aagaaactct
    22861 tttttattaa tgtatacatt ttgagatatt ttcttttttc ctattcctgt aggtattctt
    22921 ccaacaataa gttttttgat attaatccct tcttctatca ttctatttgc agtatctaaa
    22981 tttttaatta atacaaaaac gttatcatca gcttgattat tataaaaata tctccttgat
    23041 tcacttacag ttaatattga taaatcaata ttctttggtg ctgccatttt tatcaaacct
    23101 ttagcaataa catcattggt tgtttcatca tctactacta ctatcttttt tacacctaga
    23161 tgcttaaccc atttatatat aacttgacca tgtattaatc tatcatcaca tctgcaatat
    23221 tttactaaag acatttttaa tcaaactcct ttttctatta cattgagcta agaactttgt
    23281 taatattgtt tataccagtt tgtcctgctt caatacatgc ttctgctaac tcttctaaac
    23341 tttttccctc tctattaatt aaagcttcaa gtagcattgg aagatttact ccagtaagtg
    23401 catgaaactt gccacctttt ttaaggttta aacttgcaac attacttggc gaacctccta
    23461 ataggtcagc taatactaaa actccgtcac cttcatctaa ttcatttaac ttttctacaa
    23521 aatttttatt taagtcttca atattatcat tcctatttag tttaattgct gctgttcttt
    23581 ctgctggtcc tgcaataagt tcacagcttt ttataatttc ttcactaaaa ctcccatgag
    23641 tcataactat tattcctatc accaatttca cctcactttt cttgtcactt tttatataag
    23701 caattgccgt gccaacttat gtgtatcaat aacaaatggc tattttacag gatttaatca
    23761 aaacttttta ttcaattttt tttaaaaact tattatttcg atatgtatca aaagattttt
    23821 tcaacctgta tcaaatctgt atcaaaaaaa gtaaaaaaaa gatagccttt tggctatttt
    23881 taaattttgt atcaactttt gaatttatct gtatcatatt gtgattcaaa tatatctaat
    23941 atatttgcat attccatatc tgaaataatt atatcgaatg tctcttctac tagcttaaaa
    24001 ctttctttta taactaaata taattcttta tttctactaa ttctttcttc atatgattta
    24061 taaactaaag actcttttat tattactctt tcaatcatac atgaacaatg aaatataaac
    24121 tttactaaaa gttcatcttg aattactaaa gaatagtgtt cactaattct attaattact
    24181 tcttttaata taaaataagc cttttcccca tttaaaaaag tcaaagtttt actaatatta
    24241 tcaattagta atctatcaaa atatctacca ccattatctt tttcagcata tgtaacttcc
    24301 atatctccat aatgtttctt tatagattct ataatattaa aatataattt ctctaatgtc
    24361 atttctggag ttagagcctt tctagttgct tctattacaa taggtgtact taccatatct
    24421 attgctttta cttcttttcc tgtagcatca cgaataactt tagcaaatgt taaaatagac
    24481 cccatatcag ttaaaattaa aattcccttt cctttatcaa ttttattggc tatttctaca
    24541 gcttgtacta aaatatcctg aactctatct tctaatccca tattaattgc cactgcatgg
    24601 ttacaatcca gtaaagtatt ggctacattc accatgctag atgctgtaga ttcaccatgc
    24661 attatgacta atattgctat actctcctca tttaatccca catacgataa ataaagaaat
    24721 gttgctaaaa aaattgcttc atcttctgga aactctactc catacattaa tgacatgtta
    24781 gataatattt cttttgctat gttatattca tatgctcttt ccttcataat ttcatcttta
    24841 ttaggatgct ttactatatt tccaatcttt attctttcaa ttaaattctg aatatgtaaa
    24901 gctatagctt ttattaattt atctaatcct tctcttttgc tccatttatc atgcttttgt
    24961 aaaatatatt ttacagtagt tgcaattgaa tcattaacaa tgttgttata agctgtttga
    25021 ttactactat tatatataaa agtattcatt aaattatagg aatacttctg tatgtctcca
    25081 tctataacac ttctaatttg tgactcttta ataccttctt gttgaagttt attccaagtt
    25141 tcttttatca ttccataaaa atctaaatca tattttttat tttctattaa taaattttca
    25201 ctttcaaaat ccttttcttc tggtaaaaaa ataatatcat cttttccaat aaaactgaaa
    25261 ttttgtacaa actcctcttt tctattattt ccatataatg attctctaat attatttggt
    25321 agaagagaga gccttacata tacacattcc cttttatagg tcatataatc taaaaaagct
    25381 cttgcacaaa ttaattgtat atcgcttttt aattggccta tgtttccctt acacttatat
    25441 aaaataaatg ccttaataac ttcttttgaa acctttaact taacaccaat cctaacactc
    25501 tcttctttaa aaaaataaca tataagcttc attctctctt gaaagcctct ttcctctaaa
    25561 gacggaatct ttatagttac aggtattctt cttaaaaacg tttccaacat tgctgtttct
    25621 gggtcttctg tagtggctgc aattatcata actctacact cgtgtacctt attagcctct
    25681 cctaaccttc tatatatccc tttatccata agcagaaata gcatttcttg tccctctgca
    25741 gataatctat gaacttcatc taaaaacaat actccattat tagcttcatg tacaatacca
    25801 tacttatctt tatcagctcc agtaaatgct ccttttacat atccaaaaag ctgcgctaat
    25861 aataattgct gattatcaga ataatcagca cagttaaata tgataaatgg tgcattatca
    25921 ctatcttctt ttatagttaa actatactta tacatatatt ctgcaaatat agatttacca
    25981 actcctgtag gtcctattat taatgtactc aaccccttag gtggatatat tactgctgct
    26041 ttagcctgtt ctatttggga tcttaaactt ccatcacatc ctactaaatt aaaaatattg
    26101 ttctcttttt ttatactctc taaaatatac tcatctttga tagaatcatt ttcactatat
    26161 tcgtttatag agttaatatt atcaaatatc cctttattta gtttctttcc taaaatactc
    26221 tcaatacttt ctttatataa gtactgaaca ggcttaccct ttatctttat aatgatactt
    26281 tcttttatta attgatttaa ttccttacta gcattacttc tatttatacc tacttcagta
    26341 gctatatatt ctgcatcaaa tccaaatcta atgccattat taaagttcaa gtgttcttct
    26401 gttaaccttt tcaagcaaat taaaactttt tctttccttg atagcttctt ttgtgtcata
    26461 gttaacctcc cctataagca gtaatactgt taaacattta ttattaagct taattctaca
    26521 atattttaat atttttgtct aattatgtaa ctattgattt tctttcctat tcgttttata
    26581 atataaatta cgttaccctc atcttttata gaggaggaat ttatatgaat tttttatcag
    26641 atttacacac ccactctata gtcagtggtc atggctatag cactttacta gaaaatatta
    26701 attattgtaa agaaaatgga attaagatac taggtacatc tgaacatggt cctaagatgc
    26761 ctggttctcc ccacagatgg tactttcaca acattcaaaa tatcccaaga atcataaata
    26821 acattataat tttaagaggc tgtgaagcaa atatactaga tattaaagga aacatagacc
    26881 tagagccttt tgtaattcct cggttagatt atcttatctt gtcatttcac gaagctgtgt
    26941 tttctccaaa tacattagag aataatacta aagctttaat aaatgcaata aataagcatg
    27001 ataatattga aatattagga caccttggaa acccaaacta cccaatcgac tatgaattaa
    27061 tcattaaatt agctatagaa aagaatatct taatagaaat aaataattgt tctattaaag
    27121 gcgtgtcaag aaatggtagc tcggataact gtaagtatat tgctacactt tgcaaaaaat
    27181 atggtgctaa aatcatacta acttcagatg ctcacatttg ttttgacata ggtaactatg
    27241 agtattctga aaatatctta aaggaaatta attttccaga tgaattaata atgaattatc
    27301 ctaaaaaact tattaatcat tttcatcaaa aaggtaaatt actagatgtt gattatacaa
    27361 atatctaagt aataatacat gcttacttta actcccataa aaattttaat actatttata
    27421 ttatatgaaa taaagaaatt ataataaaga agctatttaa taaagcttaa ttacttttat
    27481 gaacttgatg gattgatatg gtctatgata gcaatagaaa ttataacatt tttaataagc
    27541 ttagttttat ataccttata gtataaagct aaaaaaaaga atcatctaaa ataaagtaaa
    27601 tcttatataa ggtttacttt atttttattt gtaataatca tcaatacaaa ttattttatt
    27661 atatgtaatt atttatgtct taaccaattt tatcgaaaat aacagtcttc tcttttaaag
    27721 agatttttcc tacttgatat ccatattcat ttaattcctt tttatatttc aaaaacgaat
    27781 ggtctatctc tatcccagca atatctttta tttcttcaaa agttaattta aacgattgac
    27841 tattgttttt ctt
//
LOCUS       DAELTA010000037        27838 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.037, whole genome shotgun sequence.
ACCESSION   DAELTA010000037 DAELTA010000000
VERSION     DAELTA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.037"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(139..1284)
                     /locus_tag="KPG13_001376"
     CDS             complement(139..1284)
                     /locus_tag="KPG13_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HBG0985112.1"
                     /translation="MATKLEENAKLREVIDTLETTKDDLQTVKNNIADVLGLPFTGND
                     KLETTKNTLSSIRSTFVSNLSKKGVSTVSNAPFKTLAENVGKIEQGNMIVPPWYVPKN
                     IFIKALSTAVKDKTPCVSAEKKIYFINGYTGTSGLNNLCYDTVLNTTTTKNIPSSFVE
                     SALFFYDGYIYKIGGFSGYSAPTAICQKYNILTSSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSTYKIVVFGL
                     KSSISSHYFSTINYDPVSGTYSYAGEGFDSNTFAVQLGSTLYVSDYGYKAGSGKCGVV
                     IFSSSGIKKLTFDKGAVPNYSYFGASVDGRQIYFILNSEITCFIPEL"
     gene            complement(1301..2377)
                     /locus_tag="KPG13_001377"
     CDS             complement(1301..2377)
                     /locus_tag="KPG13_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG0985113.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRFTSDKLDGVLE
                     EIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(2389..3006)
                     /locus_tag="KPG13_001378"
     CDS             complement(2389..3006)
                     /locus_tag="KPG13_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBG0985114.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(2999..4048)
                     /locus_tag="KPG13_001379"
     CDS             complement(2999..4048)
                     /locus_tag="KPG13_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG0985115.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(4049..4468)
                     /locus_tag="KPG13_001380"
     CDS             complement(4049..4468)
                     /locus_tag="KPG13_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG0985116.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(4473..4733)
                     /locus_tag="KPG13_001381"
     CDS             complement(4473..4733)
                     /locus_tag="KPG13_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBG0985117.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(4747..6639)
                     /locus_tag="KPG13_001382"
     CDS             complement(4747..6639)
                     /locus_tag="KPG13_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG0985118.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(6632..7333)
                     /locus_tag="KPG13_001383"
     CDS             complement(6632..7333)
                     /locus_tag="KPG13_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBG0985119.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            complement(7349..9742)
                     /locus_tag="KPG13_001384"
     CDS             complement(7349..9742)
                     /locus_tag="KPG13_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBG0985120.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(9807..10322)
                     /locus_tag="KPG13_001385"
     CDS             complement(9807..10322)
                     /locus_tag="KPG13_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0985121.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   10453..11274
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10518..10546
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(11350..11499)
                     /locus_tag="KPG13_001386"
     CDS             complement(11350..11499)
                     /locus_tag="KPG13_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985122.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     gene            complement(11551..12366)
                     /locus_tag="KPG13_001387"
     CDS             complement(11551..12366)
                     /locus_tag="KPG13_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HBG0985123.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            complement(12484..12666)
                     /locus_tag="KPG13_001388"
     CDS             complement(12484..12666)
                     /locus_tag="KPG13_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBG0985124.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            complement(12853..13377)
                     /locus_tag="KPG13_001389"
     CDS             complement(12853..13377)
                     /locus_tag="KPG13_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985125.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
     gene            complement(14606..14767)
                     /locus_tag="KPG13_001390"
     CDS             complement(14606..14767)
                     /locus_tag="KPG13_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985126.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(15108..15548)
                     /locus_tag="KPG13_001391"
     CDS             complement(15108..15548)
                     /locus_tag="KPG13_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0985127.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(15620..16090)
                     /locus_tag="KPG13_001392"
     CDS             complement(15620..16090)
                     /locus_tag="KPG13_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG0985128.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(16107..17417)
                     /locus_tag="KPG13_001393"
     CDS             complement(16107..17417)
                     /locus_tag="KPG13_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBG0985129.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(17418..17594)
                     /locus_tag="KPG13_001394"
     CDS             complement(17418..17594)
                     /locus_tag="KPG13_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985130.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            complement(17587..18024)
                     /locus_tag="KPG13_001395"
     CDS             complement(17587..18024)
                     /locus_tag="KPG13_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985131.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(18017..18442)
                     /locus_tag="KPG13_001396"
     CDS             complement(18017..18442)
                     /locus_tag="KPG13_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBG0985132.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(18442..18789)
                     /locus_tag="KPG13_001397"
     CDS             complement(18442..18789)
                     /locus_tag="KPG13_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985133.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSCKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(18783..19184)
                     /locus_tag="KPG13_001398"
     CDS             complement(18783..19184)
                     /locus_tag="KPG13_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985134.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            complement(19194..19427)
                     /locus_tag="KPG13_001399"
     CDS             complement(19194..19427)
                     /locus_tag="KPG13_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985135.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(19427..20326)
                     /locus_tag="KPG13_001400"
     CDS             complement(19427..20326)
                     /locus_tag="KPG13_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBG0985136.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(20338..20901)
                     /locus_tag="KPG13_001401"
     CDS             complement(20338..20901)
                     /locus_tag="KPG13_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBG0985137.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(20971..21219)
                     /locus_tag="KPG13_001402"
     CDS             complement(20971..21219)
                     /locus_tag="KPG13_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985138.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(21221..22756)
                     /locus_tag="KPG13_001403"
     CDS             complement(21221..22756)
                     /locus_tag="KPG13_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBG0985139.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            complement(22746..24176)
                     /locus_tag="KPG13_001404"
     CDS             complement(22746..24176)
                     /locus_tag="KPG13_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG0985140.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(24191..25444)
                     /locus_tag="KPG13_001405"
     CDS             complement(24191..25444)
                     /locus_tag="KPG13_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBG0985141.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYSKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(25422..26114)
                     /locus_tag="KPG13_001406"
     CDS             complement(25422..26114)
                     /locus_tag="KPG13_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985142.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(26184..26693)
                     /locus_tag="KPG13_001407"
     CDS             complement(26184..26693)
                     /locus_tag="KPG13_001407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985143.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(26714..27688)
                     /locus_tag="KPG13_001408"
     CDS             complement(26714..27688)
                     /locus_tag="KPG13_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985144.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
BASE COUNT         8357 a         5249 c         2969 g        11263 t
ORIGIN      
        1 acttttgtaa tctttgaatt tactacattg tatattaaag taaattaatt taaaatataa
       61 agctgttttt agaatagttt aaatttctgt cccttttact tttttaagta accatttcta
      121 aacaaatagt tactaatttt ataattctgg tataaaacaa gtaatctcac tatttaaaat
      181 aaaatatatt tgcctaccat caactgaagc tccaaaataa ctataattag gaacagctcc
      241 tttatcaaaa gttaattttt taattccact actagaaaaa ataacaactc cacatttgcc
      301 acttccagct ttataaccat aatctgatac atataaagtt gaacccaatt gtacagcaaa
      361 agtattacta tcaaagcctt ctcctgcata actataagtt ccacttacag ggtcgtaatt
      421 aattgttgaa aaatagtgag aagaaataga actttttagc ccaaacacaa caattttata
      481 agtgctgact ttagcgcaaa caaaataatg cgattgtttt gaatctatta tagttgattt
      541 tgtagaccaa gtgtttaatg ttgtattata atattcaaca actctgactg cttgagatga
      601 agaagaatct tctccgccat acaaaacatg tatttgattt gaatacattt cacaacaagc
      661 acttcctctt gcggtaggca tatttgctat ttcagtccaa gaactagtta aaatattata
      721 tttttgacat atagctgtgg gagctgaata tccactaaaa ccacctattt tatagatata
      781 tccatcataa aaaaatagcg ctgattctac aaaggaagaa ggaatatttt tagttgttgt
      841 tgtgtttaaa acagtatcat agcaaagatt atttaaacct gatgtacctg tataaccgtt
      901 tataaagtaa atcttttttt ctgccgaaac acatggtgtt ttatctttaa ccgcagtaga
      961 caaagcttta ataaatatat ttttaggaac ataccaaggt ggaacaatca tgttgccttg
     1021 ctctatttta ccaacatttt cagctaacgt tttaaaaggt gcattactca cagttgatac
     1081 acctttttta cttagatttg aaacaaaagt acttcgtatt gaacttaatg tattcttagt
     1141 tgtttctaat ttgtcatttc ctgtgaaagg caaacctaga acatcagcta tattattttt
     1201 gactgtctgc aaatcatctt tcgtggtttc taatgtatct atcacttctc ttagttttgc
     1261 attttcttct aatttcgttg ccatttttca ctctccttta ttaaatcata tctattaaat
     1321 tattcactat agttattcct ttagttcttt gaccacttat ttctaccatt atttcctcta
     1381 atactccatc taacttatca cttgtaaatc tatcattagc atctgtaata cttatactgg
     1441 tatctataag ctgtatactg ctaatagaat cctctatttt cttagacgaa taagtagtca
     1501 tttcagacac tctgttatca tccacagttg cattaataaa atgagtttct gcatttccat
     1561 ttatcacata aacgtttaat tctgaccttg cttcacttct aatttctata gaattatcat
     1621 ctataatttt aaagtttgta actacatttt cttttgtagt agcatctata atatttacaa
     1681 ctattctctg tgttaataaa ctatgtgtta cagttgcttt gaatccactt tctgcatcct
     1741 caacccaatc atcaattgcg accctttgag tgtatgcaac atttgagcca cctgcgatta
     1801 attggtcgat tttagtattt aactctgttt tagcagtttc tatgttgctt gttaattctg
     1861 ttttagttgt atcaatttta gtattaacag tacctatttt agtttctaag tcttgtatat
     1921 ctttgagtgt tgcaaagatt attgttgggt caattttaag ttctatatta tttacattag
     1981 atacaataag cacagtttta accttcatgt ctaccactgc accttgttct atagaaggtt
     2041 tataacactc tttgtattta gaaatggcaa ttaaattatt ttcatcatct aaatatccta
     2101 tttctcttat cataaacccg cctacacttg atggtattaa actctctaat attatacaat
     2161 ttggtgcagt ttcatctgta gttgtatttc caatattgcc ttcccatacc acgtttttga
     2221 gagctgtctg actctcagtt ggagtatatt cactccctcc tccatcacca agttgaattt
     2281 ttacaaatcc cactttatta cctgtgacac ttgcatttgc tatctttgct ttccctacat
     2341 ctgtaattat agtgtaataa cttttatcta tagccaatat atcacctcct aaaatattgt
     2401 tatctcttgg tatccaactc cattgccagt taatacatca atttctccat aagtttctat
     2461 atctggtgga ctccaagggt atatagttat ttcttgacct attagggttg ttataccaaa
     2521 attcatataa ttgtctttac ttacaagcac tctagtgtaa tctaaagtca tgttgcaagg
     2581 tttaatacta cttacaaaag aatgaacctc ctcaaaccaa tcttgatttc tagcatcact
     2641 ttcaagatgt atattataag tagcattatt aatagttaat tcataattgc cttctccaac
     2701 tatactatct agccagttcc ttaaaaatct ctctgagtaa ggtagtttac ttatatattt
     2761 actaaaaatc ctaaaccttc tatcttctaa actctcatta cttttaggag ttatagacat
     2821 tatcttttcc catcttttta taccacttgg agttaaatcc tctaaaaact ggtcacttga
     2881 taggtcattt aatttttcat gtagtgtttt tatttctttg ttttctacat taaatacttt
     2941 tatatattct tctttatctt gtagaatttg tggtaagtaa tttattagat taatctcttt
     3001 atccaactac ctcacctctc actactatac tgttactatc tattgttaga ttagatttaa
     3061 cctcatttat cattgtattt gcaatgtcta atactccatc aatactaagt aatctagttt
     3121 caatttgaga tatacggact attaagtttt cttcatcttc ccaactcatg ttaagttcat
     3181 ttaaatagtc gtctattgct tcttctgcaa ttgattttat attctcccaa gtgtagccat
     3241 ttttgtatgt tatctctgct gatatattta tagttgtact tgtaactcct tcaactgtga
     3301 ctcggtgtcc aattggtgct aatccaagac cttctccttg gttttgtaga ggatcaattt
     3361 cttcttgaac taaattaact aaatcactag atggaacttt gaagttagaa ttaattatta
     3421 ctaacttaac agtacctcca ccgtcccaca caggataaac cttaactcct ccaacatctt
     3481 gtattttgtt aacttcatct ttataatttt gcatatttcc accaaaactc tgtgagttta
     3541 ggctatcata atatctttgt cttaaactat cttcactttc ttcatcttct ccattaatta
     3601 gtatttcagt taactcagca gtttctaatt tgtctacata ttcaattgga atcaactgac
     3661 caagttcaaa tacaggtcca gtagtttcac atttcatttt atatgttttt tcagatattc
     3721 tctcaattgc cacataattg tattctccta agttgaatct tgaatcaata ggaatatcta
     3781 tattaaaaac tccttttgca attgtattgg ttgcaggtaa aggtgtaata cctctttctt
     3841 tacatctctt ctctaaatag taataactag cagtatctac aaaagtttgg tctagtaatt
     3901 catccatggc aatgtatgtt tctgtaagtt ctatagcaac aggagcaaga gcattatata
     3961 ttatagaacc ttccctttta tcaaaagtat ctggtacact atctaacatt cttttaatta
     4021 tattttcaaa tgtcattaac tcaaacaatt atacactcac caccttctct gcttttatat
     4081 ttccatattt actgtaaact gtgaacttac aatgtacttt accctttaca ttttgaaact
     4141 caaaattatc tacattttca accctatcat cttgaattag tgcttctttg attcgtcttt
     4201 caagttcggg gattacaaag gatataggct ctccaataag gtcgttcaac tcgactccat
     4261 aattccaact atatattaga tgttggtatc tctctgtgtt taaaattaaa aagatggttt
     4321 gttttaatgc ttcaacatca tcacaaatac catctatctt agatttttct atatttaatt
     4381 taaaggtctt acttggttct tgccttacat caaaattaat tattgataca tcttcaatgt
     4441 cataatctaa attatcgctt ggtaacactt catcacatcc tatccaatac taaatattgt
     4501 tgtcctcctt gcatacgaat taagactact ttatctccta tttttttatc tgtatatctt
     4561 ttaaatgtat ctgtttgtat tagaaaaatt tcaccaatag atagtttttg ttctatctta
     4621 actcttagag gattaatact ttctattgtt ccaaatacaa ccctcattgg gttgcttgtt
     4681 tctactgcat ccattgcagc tttttttatt atctgtaata aatcttggct catattgtca
     4741 cctcacttat ataaatcttc tcacatgtgt gtatgctttt ccttttctat aagaattaac
     4801 agactctatt tttaccacat ctcctgtttg tggtgaatga attatttgat tgtttccaat
     4861 atacattaca acatgattac tacttcctcc accaattcta cataataagt ctcctgcttt
     4921 ccacttgctt ctatctttta aatctactgc ttttcctgct ttactttgtg cagaagcggt
     4981 acgaggaatt tttataccta tttgtttata acaccattga gtaaacccac tgcaatcaaa
     5041 attattagga ccttctgctc catacacata attacaaccc agtttacttt ttgctatact
     5101 aattaattta tcttctttag agttattatt tgtactactt tggttattac cttcaacttg
     5161 atatgtttgt tcttcatctc cacctataat tatatagcca ttctttctac caaatttttt
     5221 acattcacta gcattagcta atagtatatc tatatgatat gttccgtttg tttcaacata
     5281 tattcttcct ccattatctt taactgtata tactttgttg tcataggcag taccaggaag
     5341 tataattttt actttatctc catattcaaa aactggatgt ttctttagaa aatcatcagt
     5401 ataccaagtt ttcttaactc cttctcgatt cattggacca gcaacagttc ttgattttac
     5461 atcaagtggc tttccattgc aatctgtttt tccgccttcc attgcattgt tccctggata
     5521 atatgcagta aatatagcag gaactttttt acctgtattt tttttcgtaa tactttgtgc
     5581 aggaccattt ttcttttcat ctttattatt agtatttcca ctactatatg agcttgaaga
     5641 atatgaagca aattcgtctc catcaacaag ggtcaaatcc ataaagtgcg aattattttc
     5701 aaatgtatgt tttactttct caactaacat ataattttgt aattcaatat ctcccaaatc
     5761 taaaaaaaca ggtactaaac aacctgctct cactctaata tcaccaagta cattttttaa
     5821 acttaatgac ttagttttct tattatatag ttttagaagt atatcacact tttgttttat
     5881 ctctgcttca ctcatgtttt tgtctactgt atcaaacatt tgaagtattc cccaactcct
     5941 catatgcgtt gagtcttgag caatatacac atctcttttt cctgtttctt cattatctct
     6001 cacaagttta atctttgtgt aagtatcact atctattgat gaattatagt caaaatcttc
     6061 tattacatca ttgttcataa cagtatctaa tttcattgat gcaacattct ttaatgttat
     6121 tcttccaaaa tcatcatata acacatacat ttcttttttc tctcttagag tatcatctag
     6181 tgctgttagt atcatgtcaa agagtgtttt attttcttcg actctagata gtttatactt
     6241 agtatcttct atgacattgt attttaaatt aaaatcttta gccaacattt ttacaagttc
     6301 acttgctgtt ttattgctat acacataagt atctttgttc ttaaaatatc ttaactggtc
     6361 gtaagcaaca attttaatgt gattttcttt atctcttttc ttctgaaata tatatccata
     6421 gaatatacct acacctttgt aatatagtct tacagagtta ccttcacaaa actctaatat
     6481 atcatccatg actattgtaa attcaagctt agatggtgtt cctcgtctct ctatttccca
     6541 tgtgatacca tcaatgacag caggttcata gaaatcttcc caatgtgcta tgattaatct
     6601 tacatctcta tcattcgcca gaactaaatc atcagccaag ttttaacacc tgccctttgt
     6661 aaatagtgta tttacttaag tttttgccct tatttgcctt atccatcata gatttattta
     6721 gttcgtatac tttcttatat aatgaaccat taccaagttg cttcttgcag attaaccaaa
     6781 ggctatcccc tgcttttact gtatatgttt taccatttgg cttattggat gaatctggtc
     6841 taaattcttt tggtttcatg actggagggg gagtcctacc atagtttgtc ttttcaggag
     6901 ttgcaggaac taacttttta gttgagtaat ctctatattg ctttaacttt attgcaactt
     6961 ttgtatctga gccattttct gcatcttcta ctatgttata ttcctcaaga gatactttca
     7021 tattagtgtt aaatagtact ttattaccta attccctcga tacaataaat tgaaatggct
     7081 tacaatcagt ttttagtaat tctagtttac ttaaaaagaa ttgaacatct ctaaattgac
     7141 ctctacaaaa aggtaattta ttatgtgtaa attctgcttc aaaacttatt tcagataatc
     7201 cttctttttt taatatattt acttctccaa catttattaa atcaactgtc ttgtttttat
     7261 ttgtaacttt aatctctaac tttggcggag gtattggtaa ttgtactcca tctaaataaa
     7321 agtcataagc catttatatc cctcctctct aaactattcc ctcagctgat acaaccatag
     7381 cgtcgtttaa tttttctgtt agtacattta ctattccatc tacatctgcc tcactattta
     7441 tgttgtttgt attgttcatg tcaattttaa tgtttactcc tgtgaatcta tttattgttt
     7501 cttgctctgc aatatctcta agatatttta agtcttcttg acttttatcc attgttttag
     7561 ccattttggc agtatttccc gccgtgtcct ttacattttt tcctaaatct ccaccaaatc
     7621 ctccaagatt attaagtgga ttgtctttat cccacaaatc ttttaatcct agcttatctt
     7681 ttgcatcttc tagcatctta ttaatgtcaa aagtatcttt taatttatta gatatagcat
     7741 tttgccattt agttccaagt gcatttcctt tttgaaattc tgctccaata tctttgtatc
     7801 ccattcgctc caattttact ttctctggtg cgtctcctac ccatctgttt aagctatcaa
     7861 tctgttgttt gatgtaacta ttatctgcct taactggcgt aaacgttgcc tctcctactt
     7921 taccaatatt tatccctggt attttattta ttaggtcaat taacttattt acccctctta
     7981 ttgctatatt tgctccatcc acaaaagcct taccaagtgc gtttcctgca ttatttacag
     8041 aatcattcaa tgatgccatt ttctcaatta taaaaattac accttttgca attgcttgtt
     8101 tcatcaaata tacacactga ttccatccat tcgcaattcc ttcatttaca tctacacatc
     8161 tattaaatag ccatatcatt atattttgta ttgttgcaac tgctgcaaat actgcaccta
     8221 caatcacccc tagaacagtc aaagacgtac ctgcaaattt atttattgcc gctactacca
     8281 taaatattac agctattact gctataaatc caagaactat ccacacgagc gggcaagcat
     8341 acaacgctgc atttaggcca tattgtgctg ctgttgcctg ggctgttacc atcgcttcta
     8401 tagtttgtgc agatatttta ccaaataaag ctaattcttg tgctgccaat gctgcacaag
     8461 ctttatatgc ccctaaagca tacagggcat ttacaaaaga tgatactcca gttacaattg
     8521 cacttgcaag taaaattcct ttatatattg caatagctgc gacaactcca taaacagcag
     8581 gtgcaataat actccaattt tgagcaaaga cattagcaac atttagtgca gcattagtaa
     8641 tccatcccaa tccttgtatt atcagtacag ttcctgttgt aattccttgt acaaaatctg
     8701 aaaaaaaatc actatttaat atatttttta taatatttaa tgtatcataa atgtttgaag
     8761 atattgcaat cataacattc cgcatagata caacaatctc actaaaacgt acactttgca
     8821 aagctccgct tatcttactg agagtctcag aaaatatcat ataagcatca tttttcatca
     8881 ttgtgagagc ttgagaaaaa gtgattggca tacttgcaaa tttcttttct atctcatcag
     8941 aagctttaaa cagtgcattt cttataacat ctgctgttat tgctccgcta cttgataatt
     9001 ccttcaattg gtctttagtt tttcccattg catcagctat tttagtggct aataagggtg
     9061 cattttccat tatggaacgg aactcatcac cttgaagttt tcctgcaccc atggcttgtg
     9121 ttaattgata cattgctgca ctagcttcat ttgctgatgt tcctccaatt acaaaagctt
     9181 tattcataag ttctgtaaac tttatcacct ctgctgaact tccaaaagca tctcctgcaa
     9241 gtattccaag tttagcaatc tgtgctgctg tatctgcata actagctctt gcactttggg
     9301 ctgacaaata aatcatttta ttaagctggt ctgttgtctg taagccatca ttcattagat
     9361 ttaaacgtgc ttttgtactt gcaattgtat ctgctgcttt tgtaatactt tctattcctt
     9421 ttattccaat gtatatccca gcaatacttt ttaatttaga cactaataga gaacctgctt
     9481 cactaccttg ccttatttta ttattaaagt tttcctgctc atttgtattt cttccaattt
     9541 tctgttctat ccttgtgaga atactttcta tgttattcaa actttgttga gaagcttgta
     9601 tattacctgc attgagtgga ttattcaatc tgccttgcaa tctctctaaa ctattaattg
     9661 ttgtattaat agaattagtc atattacgaa atgctggtgt cattccgtcg aaaatcttta
     9721 ttgaagtttg tattgtagcc atttcctcac tctcctttca taaatttcca tataaaaaaa
     9781 cacctacatt agtaagtgtt tattatttat gcccaaaact gtttaccaca gctcaaacat
     9841 gtaactctaa ctttctttgc tcctaaattt ccagctacta aaccaatgcc tcctgctata
     9901 gtagcccctg ctacagcttt tcctatgcca aaacctttct tatgagcaga caatgaaaca
     9961 gagccacacc tagggcaacg agcaacatca tcttcaagta cattagtttg tttatactcc
    10021 tcttccctca atatctcttt ttcctcttcg atatttattt tttcacttaa ttctttaaaa
    10081 gctggatata taatattttt acactctttt atgtccagcc ctagtaactt cattgcaata
    10141 tcaattgcta agattttatc tcctttagtt tctttataaa tattagtcag atttaattct
    10201 tttccatgaa catttataaa taaatcatca ttgttaactt tatctgctaa tgtagctgtt
    10261 ccgcaatttg agcaaaattt gcctgcacca gttatctcat aaccacaatt tgaacaaaac
    10321 acaacaatca cctcacataa tacttatttt atagcactat tatactatat acgtaaaatt
    10381 tttacattat tatcacctcc tttcataaaa aaaaacactt actcatttgt aagtgttttt
    10441 gaattatttt taattttaag tccacatagt taatataaaa cgagcatcat ttatcattgc
    10501 taatcgtgca tttgtacatt tacataccac atagttaata taaaaccata cactagattc
    10561 taccccaaaa atgaagctca atatatttac ataccactta gttaatataa aacgcgttaa
    10621 tgcagtacca taacgatatg ggactaacta atttacatac cacatagtta atataaaacc
    10681 gtataggaat gaatgcaggt agtgcatttt ctagtacatt tacataccac atagttaata
    10741 taaaacacag gtgtatttac ctatagaaca acaagagtta catttacata ccacttagtt
    10801 aatataaaac attcaagaag taactgtaat tgttcatctg taaaataatt tacattccat
    10861 atagttaatc taaaacctgg tcttaaaaac atacatgttg caaaagtgaa tgatgaattt
    10921 acattccata tagttaatct aaaacgaaca ctaacaaaaa agctaaaagt ggtcactata
    10981 ttatttacat tccatatagt taatctaaaa cgaatgatac attagaatat atattttgta
    11041 gtgaatgatt tacataccac ttagttaata taaaaccatc ctacttagat actgcacaaa
    11101 tagtatcgcg aatctttaca taccacttag ttaatataaa attgaccagt acgaatgcga
    11161 ggctagcaat gataaacgac tttacatacc acattgttaa tataaaaacc ttgagtgacg
    11221 cagcaaaagg ggcaaaggat acagcattta cataccactt agttaatata actcctacct
    11281 atattatatc attttttact acataaagca cttgaaacag taaaatattc aagtgcttta
    11341 tttgtctatt tatttttctc tttttttcct acattcttct ttcactaaac ttacaaatct
    11401 ataaaattca tctggatttt catttttcat tttttcaaag atatttccaa gttctttgat
    11461 aagttctatt ctatctatat caagtaaatt ctcatgcatt gtattaatcc ccccaaaact
    11521 aaactaaaat atttaataca actgataaat ttactcaatt ttataaacca catgataatt
    11581 cttcttctca cctgcaatct tggtaggtct attattctcc tctatccaat ttcttatttt
    11641 atctattaca ctttctgtat acttatttac agtaccagtc caacttccat tagtctccca
    11701 aactccctta acttcactat cttctaaatc aatcttttta ataatctcac aaacagccat
    11761 ctgagctggt ttattactct tagaatatag ttttaactta gatgctattt gttttgtgtc
    11821 aaaataatgt tcttcttcgt ttatctctat tggcaaatca attcctgcct ttttatataa
    11881 tgttttagct gtaagtaact tagatttatt gtcaaaacct gcaccatcca atagttcttt
    11941 taacatagat gtgctgttat aagccagttg taatttttca atctcacttg ctttttctct
    12001 tagtttttcg gggtcagcat tatttgttat gtatgcacca gtttgtcgaa tggctggaag
    12061 tacttcatca cttatccaat cttgaaatct ctcagcttct tcttttttag atttaaatat
    12121 aagtttataa acaccacttt cagttagaaa attttctcct gtattatgca attttctaaa
    12181 ctgcatatta tgcattttag aattagtcaa cttaactact tgattatcat taaattttct
    12241 aatactactg ttcacatctg aaatatctaa acactctgcc acatgctttg gattaaataa
    12301 aatttgccct tcaaattcga atacctctat atcttttcct tcaaaattca ttaattcatt
    12361 cataataaat tcctccttaa atttgattgt aagaagtacc ttactatgat agaatatatt
    12421 tcatagaagg taacttcttt gggaaacagt cgcaagtgct ttggtcggtg cagcggctgt
    12481 tttttatttg tttttgtcaa tcttctcttt caccaattca attcctctaa ttacaacatc
    12541 tgttttagat attttaagat tttcagcaca ctcatttaat atatctgctt gttcttggtt
    12601 aagtctaact tcaaatctta attttttgga attttctttt ggtggtctgc ctaatttatt
    12661 ggacatctta tcacctctct ttttattgtc cgtacttaaa ttataatata gtacgtacaa
    12721 taattcaaga gttttttact aattttttct aattatttta ctcaaccgac caattttgag
    12781 caaaacaaaa gcacctacca aaaagtaagt gctttctata taacagattt aattttaaaa
    12841 taaagtattc acctaatttc cagacgagaa atccaaatca tcaattaaat ttacattatt
    12901 ttttatatta ttccaatcat ctaaaaatac attataagtg agtgtcacat caacaataaa
    12961 gcttattctc actgaatgga tatcattcat atcttttata ttgtttacaa attctttagt
    13021 aaagtttcta cactcatttt catcattaaa cctagaattt tccacttgaa tactaactat
    13081 atatccctca ccttttgttg ggtttaatat gttaacataa tatgttgttt tatcttttaa
    13141 gtcttctggt atcatagagt ctacttttgc ttgcatctct tgtttataaa tatcttcttt
    13201 ttctctttcg ctaattatat tttcttttgt aatatttcct tcatttactt catctctatt
    13261 ttttgaatct cgtatcgctt gatgaatcat cattgatact gtaaatacaa caaagtatat
    13321 taaaaatatc actaaaattt tcttcaacaa acttaatttt ttaaattttc cccacataat
    13381 gaactctccc ctattaaatt ctttattcaa atataattct atctaaaaat aagaacttag
    13441 tctattgatt taaaatattt tgagcaaaat aaaagcacct accaaaaata agtgcttctt
    13501 tcttctattt agtttttctc cacattgtta atataaaagt ttggagtttt aaaattatca
    13561 ttcctatctt ttgctttatt tacataccac atagttaata taaaattgag gtagaaaatg
    13621 ataaaaagac ttttagagta atctttacat accacatagt taatataaaa cctgttaatg
    13681 taatcatttc accatcataa gttgcgaaat ttacattcca tatagttaat ctaaaacaat
    13741 aattggtgat ggtacaggat atacaaatgc aacatttaca ttccatatag ttaatctaaa
    13801 acagaaaatt cattgtacaa ttcttctttg tattttttaa tttacattcc atatagttaa
    13861 tctaaaaccc caaaataaac ttagtatttc caatatctac acatacacac ctctctcaaa
    13921 tttgcagtga accatgagta gtgcaattga taacatttat cacacaccct caataccttg
    13981 tattccaatt gttaaacttc aatttatcgc aaatattgct cactgcaaaa tctctacatt
    14041 tttattatat cataaaaata ttatttttga atatctgtac caatttgtgg tataataaaa
    14101 gcaaggaaat aatttacttt atacaagagt agctatttcc atcaaaattg atttaaagaa
    14161 ttattttttt aaatcaccct tattggcgtc tgggtgattt tttattttgt cataaatata
    14221 agctgatata acaccagcta gtatgcttaa taaaaaaact atcatataat ttcacctcct
    14281 tccttatttg gaatttggcg tttaatatga aaataatcac ccttcgcact ttcgattatt
    14341 atccttgcta caattattat aacatataat tattacatat ttttccattt tttttatata
    14401 aacaatgaaa ttcaagtaaa taaataccta cttatttata tatattttat aaattaattg
    14461 ctttataatc aagttttcaa ttttttaata aaaattttta ttttttattt tatataaaca
    14521 atattttttc taatttgtgg tataataaaa gcaagaagaa ctacaatcta ttttgcggta
    14581 gagtgaagtt cataattaaa tgaatctatt tgaacttatg gaacttgatt ttaaaatcaa
    14641 attcccagcc acttttactc ttgccacgag ttgagtggct ttttactttt agaaatactt
    14701 tacaaattaa gcaaaatatt aaactagcaa taacgccagc tattacatta agtaaaaagt
    14761 tattcatact tcccacctcc tttcattagg aagtaggttt tatctcagta tgaactccac
    14821 tctataaatt gtagattaca tcttcttgct aaaaatatta taacatataa ttattacata
    14881 ttttacctat tctatattta tttttttatt ttgctatctt cttcgtcccc tctttctctc
    14941 tctttcagct tctttcattg cttcctcttc atcttctatc tttataagta ttgaggcggc
    15001 tgctaacgct ctctcattaa cttctaaatt catatattca cttggcttcc actttaattt
    15061 ttgaatacaa taatgagtga tgctagcatc aaaatcgcca cctctgatta gttttttgct
    15121 tcttctactt tatcctcaaa agatgtatca aatccattga cttcattcac ttttactgta
    15181 taattgacat actcacctgc tgtaagcatt gtctttaata actgagcttc tcccattact
    15241 ccataactat tttggagttc ggcatccttt aaatctggaa atactgtaga tgctacacat
    15301 aattcagcta cataactatt gtagtcaatt tcacttgtat attgtccagt atgcttacca
    15361 ttgttaccaa tcacttttac tcttttagta cactttcttc ttagtgcttc gtcttcttca
    15421 gatgataaaa cttttaattc ccattcaact ggtttccctt ctttatctaa aaatctgtta
    15481 ctcgctacat attttacatt atcaaccttt attgcatttt gacttaaaaa agcacttaaa
    15541 ttactcatat tattctaatc tccttttatt ttaatttttc atataaaaaa tacacatata
    15601 taatttataa atgtgtattt tactccattc ctgccaataa attaaatttt tctactaatt
    15661 cccaatcctc aaaagtgaaa tccatatctt catctaaata ctcaccatca gcatcaaatt
    15721 tagtaattat tccactgtcc atattacaat ctttaagtac tactgtctgt cttcctacag
    15781 cagatgtagg gtcttcattt gtaacttgta tgtcaaaata aatatcctca ccagtttctt
    15841 tatatctgta aagtaattct ctaaaaatag aagtattata atgaaatgtt gcacttccag
    15901 tatttgtact cccagttgtt ttatttccct ttgttgttct tcctagaatt ggaacttcac
    15961 ttttattttt ttccatttta gcctctaaat ctatagcttg cataaaatta tatcttttgc
    16021 cttctatagt tataaaacat tcagctttct ttgcactaac tgtatcttta gcatttattg
    16081 tttgagccat attatcactc ccctctctaa ctaactgaaa ctgtcatata aagcttactc
    16141 atagcattta taaccttaac agcatcagat actatgacag ttttcttatc atttccaagc
    16201 tctacactaa catcatcagt tttaaaatct tctattgccc ttatattctc taattcttta
    16261 tggtgtttaa caacatcatt ccagaaactt attcttcctg ccttatcatt cggaacttta
    16321 cctaaatact tttcattaaa taaagttgca atatcattag caatttggtc aagtactcta
    16381 acactttggt tacttgaaaa atcgtcattt ttatcatctg taaatgatac aaaagtattt
    16441 atatcctcta acacatgaac ttcatctccc accttgtgga atataaattt accagttttt
    16501 aaagcttctt caagttgtat ttgtgtataa ttaacatcaa catcaaactc accatcatac
    16561 tttttattag tattagattt atttatatcg catcctgcta tagctccagc cgcccaataa
    16621 attaaactag attcaactaa atctttatct ttaatcttat tttctacaga cactacacct
    16681 tcataatctg catcactttt cttatatagt actgtttgaa acttagctcc taccttatct
    16741 ctcattctct ttgtaaattc tacaaataaa cttttaattt ctgttgttgt agccaaacat
    16801 cctaaagcat taaagctata gctttctatt ttatccaaga aagcttggta ctctgctcct
    16861 gtcacagctt cgccattagt tccaccagta aatacaagtc ctgcacttgc ttctagtgtt
    16921 gcatccttct tccaagtgat atagtcattg tcttgtaagt ctgtaataac ctttgctatt
    16981 tgagtatcta ccttcttatt atctaaaagt gttacaacat caaatttagt gttatcatct
    17041 atatttgttg taactgttac ttttaagtca tttcctctga taccactata ttttgctgtg
    17101 gctatagtac aactggcttt aacgccttta tttaatttat aaaaatatcc caaccttata
    17161 tttttgaata aatctctcaa acctttcagc ttctcatgag tataatcata tccaaaatac
    17221 ttcactgaat acttctcaaa atcatcactg gttacttgaa atacgtcttt atctatgccc
    17281 caatctaact ctaaaggtat tgcaacaata cctctatccg ataatgaact ggttgccctt
    17341 gtagcactta caaaatttat atatgcacca ggtaggacct tattttgtgt tacaaatgtt
    17401 cctccaccta aagccatcta actcactcct ttcataaatt tatttattct atcctctacc
    17461 tctgagaagg aatataaatc attttctttt aaaattgcat ttaataaatc ttttctattt
    17521 acatacttct tagagttaac tatctgctcc ttagtaaact tgtagttatc ttctttgctt
    17581 aatgttttac tcaaaattat cacctctctt caaaccaccg aataactcta ctgtatccat
    17641 cttatctgtg tcattacttt ttatagtaaa gtagttataa tcaacaaaga aatgaagaac
    17701 attatctata atttcaaagt tcatatttgt acctctgact aagtctccat caatttctat
    17761 atactctaat tcctccagta acatctcagc tatctcattt atttcaaatg atttatcatt
    17821 acttttagga aaataatgta catcaaaaga gttctttttt aatgtcctgc cacttagata
    17881 ggatactttg cttggattta aaggaacaat aaaaaaacaa ggttcattta taccttgctc
    17941 cacatcttca ctataaattg tataactctc tccaaatgtt ttatctaatt taatagatat
    18001 tccatcaatt atattattaa gcatcaaata ctcctttaag caatattaat agttttttct
    18061 ctataatctt atcaacttgg ctttgtagtt ccatctctga aattgttaag aaatgttgtc
    18121 ctttaaccca accttttcca tctttagttc tatggccata ttcaacataa cttgcatatg
    18181 tggtcggatt aacaacctct ataatataat tatttccttg tttatacaca ggaagcgacc
    18241 tagcataagc cactccattc catccttgtc gtaagaatcc tgtatcaact ggtgttcttc
    18301 taattacttt cccaagtaat cgtgctgcta attctcttgc tgcatccttg caaaacttat
    18361 ctaaatcaat ctttgtaagc tcctccatct ttttacaaac tcttttaaac tctctaaaat
    18421 caacactgcc ccatctagcc attatgcttt atccttaaat aactcaagta ttatttcttg
    18481 atgatttgga tatatagctg attctccact tcttacatac tctttatcat ttataataag
    18541 ttttgaacct gctttaattt ctatatctgg agatataaag agtttaatag tttgctctag
    18601 cttagctact ttcccttctg tagcagaaac tatattttta catgaaagtt tacatggttg
    18661 attttctaat acaatcactt ctttattgtt agttcgtttt gttacagggt ctttaattgg
    18721 ctgatactca actatagtac atttatctct atataacatt tctattgctt ttctagtttt
    18781 acttaccatc ttaagcacct aaaggttaat atcttattct taccataagt agtaagataa
    18841 gctactaagc tatcaaagcg ttgttctggt gtttgagagc cacttcctat agcaaaatct
    18901 acctttgtat caccttctga tatagacttt tctacagctt caaagttaat gctttctata
    18961 tctaattgcc ccatattttt cttggtaaat aagaactctc caactatcat atcaacttca
    19021 atttctttca attcaattgg catagttttt atattacaat ctagtttaat aatattttct
    19081 attttttctc ttacaaaatc tattaaccac ttatccccat cttttaatat atatccaaaa
    19141 ctttcaagtc ttttttctat atcatcaatt atattatttc ccataatttt cacctacttt
    19201 ttagtaagtt tatttttctc tttaagctgt ttattttctt cttctaaaga ctcaactttt
    19261 gaccttaaaa tattattttc agctattaaa tcttttacat ttaatgactt gccatacttt
    19321 actgccttac cagtttcatc tatcaaatca tatcccatct ctaagaaatc atctatttta
    19381 cattcttcta tagttaatat tctatttaat ttccttactt gtgccattat gctccagctc
    19441 cttcaacaac aaattgtatt gcatcagctt ttttatttaa tataaataca tcctcaaaac
    19501 tttcttcaaa gtagaagtat tttccctctg taactgctgt tggttcgtct aacttagaga
    19561 actgataaga aacaggtgta attattgcac ttgggtgaac taaggacata aagatttgtt
    19621 tagctcctgc tcctactttc catccagttg taaaatcata tgcagttttc attagattag
    19681 atggtacttt aattatttta actgtgtcaa tatcagttgt ttgacgatta agagaagttc
    19741 ctgcatcctt tatatttact gttctttgta tctcttttgc atttttgata agtgtattta
    19801 ctactggagt aacatacaat attcttccat tttcaggtac tctagcttct gtcatttttt
    19861 ccattaactt atcaaatact tctaatacgt ttgttgttgt aagaacagtt gtatctgctg
    19921 tgttacctaa tgcggtccaa tcagcatata ttttagatat acagtaagca tccatctctg
    19981 gaaacttttg ttcctcatta tatacttttg ttatattgcc tattgaagcc acataattag
    20041 tttggtttat atctgctgga tgaaccaatg ttgaccattt cctttgatta gttaatacct
    20101 taggttccca agcattatca tagtttcttt gagctactgc tattgtatct ctgtttgaat
    20161 ctactcttcc agttgtagat atagttggta tttctattgt tttagaacca gtccatctat
    20221 atcttccatt atttggtgtt gcatacaaat ccccgaagtt taaagtataa ggatatgctt
    20281 gtgctaaaac atttgaatat tcttttgcat aatttagtgc tgccatttta tttcctccta
    20341 tttattatta ttttcatgag gtcttacccc agtaaaatta aaaccaaaat catttatctt
    20401 aggctcttgc cctggtgtta tagtatctat tttaggctct tcaccttcta gtgttgcatt
    20461 aaacaaataa tctttatcct gtttcaaagg gtttatttgc tcttcaaaag ctttttgtct
    20521 atctttacta tttcttagtg cttctatatc taaatgagct tttaacgcta tttcatccct
    20581 acatttaata gacttaaaag catcatttaa ccaataatta aagtcctttt cttcaatttc
    20641 ttttttgtag gtttcttcca aagttttctt atcagtttca taagttgttt ttagattctc
    20701 tacatcttct tttgtcatac ctccttcaaa ctttttaata gcttcattag ctgtattaag
    20761 ttgtgtttca agatttgtat aatcttcttg agtaattgta gtctctttta ttttcttttc
    20821 tatagatttt tgaagagaaa ctacatcaat tttgttattt tctattttta ttccttctag
    20881 caattctttt aaccaatcca tcttttaaat atctcctttc attttttaca taataaaagc
    20941 acctactaat ttatcattta gcaagtgctt ttacatattt actatttgta tatctttcca
    21001 taaatccttt agtaatttac catcaatgtt gtaattatca accatatcct taccattttt
    21061 ataatacttt gtatctccat tagggcaaag agtaataaac cttgtatcat catctccaat
    21121 agatatatta tatggtttat tatataagtc aaattctata tctaatccta aatcaataga
    21181 atcaattaaa tgttgtaaat tcttaaattt attatccatt ttactctcct ttcaaaatat
    21241 ctttgttagc tattttatga gcttttgtaa gctccatatc cttctctctt tttaccttat
    21301 catggttatt ttcatcagct aaccaatcat gtttatgagg tacaatttta tgttgctttg
    21361 ggtttctatg gtcagttaag tctaaatcta atctaggttt tcctgtatta ccatagtatc
    21421 ttctttgaat taatttacca tctttgtaat tatcaaatac actatttggt tttgattcaa
    21481 atggcactga atgaacactt ccactagtta aatttctctg attcttaact tgccaattta
    21541 catccttata aagcttttta gcttcctcat accttatagt atcattatac ttcatatgtt
    21601 gatattcatc aaatttagaa ggtatttcat ttcctaatac ctttttatat tcttcaaatt
    21661 gttttctatc tttactctca tttagttgca tttttctaag ggtatcagct ttttcttttc
    21721 caagtctacc ctctatatgt ttctcatacc actcattata cttcatatta gatggtacat
    21781 aatatgtttt tccatcttct ccttttgctg ctctgtaacc ttcttcatct tcaaaccaag
    21841 gagctgttgt tgtcctacaa cgacaatgaa atggtggagc tgttattcca acttgataat
    21901 ctttcatatc aaatacttta ccatctaatt ctctgcatat atttgaagtt cttaaatcta
    21961 gtgtagcaat tatctcatat ttctctacat ctaaatcatt aaaacaatct tttctacttg
    22021 ctgatgcaaa gaaagctgat tcagtcatta ttaagttctt agcttgagat ttagatacat
    22081 taaatctttt agcaaagtca tttactagat tctttggatt ttcacctcta ataattgatt
    22141 gagttagctt agtatgtagt tcatttacta aagcatgtct atgcttaccc caaattcttt
    22201 cactaaagtt taatccatct gttgcccatg gtttagagat aattttattt attctattag
    22261 tatcaagact cattaaactc caaccaacgt ttactccttg ttgaacatta aaagctgtat
    22321 gatagtatcc acttgtataa atatctctca ttagtttatc aataccatca agttcatttc
    22381 catataaaac ttctacttgt tgctgtattt gtaactttaa agcttcaagc cttgttatat
    22441 gaactcttgc actagcattt tctaactctt tcatccactt ttgatttata gcattttctt
    22501 taccatattt aatatattct tctacactcc atttaaactc ttctagttct cttgtattta
    22561 gtagtttctt agcttctaat aaagatattc cttcattttt ggcaaatctg ttgtaccatg
    22621 ctaatatatc tttttctata ttatttatag ctagtttata ttgcttttct aattcaagat
    22681 aatattttac acttttgtta ttttgagctt cttctaattg ttcaaatctc tttctccaat
    22741 aatctttatg tttcatctat aacaccatct tgattattag gaattaaatc atcatactct
    22801 ttttgagtat cttcctgttt tttaagtctc tcaagttcgt cattaacatc ctcaacccaa
    22861 ggatgattgg aaacaatagt ttcatctgat atgattccaa ttgatttagc tgccatatct
    22921 attttttcag actcatttat tatcattgag tggttaaaag taatttgaac tgatttataa
    22981 tcataacttt tactaccact tatctttaaa tactcacata caaaccacaa aagttctcta
    23041 attgcttttt taaacttctt ttcagttttg gagcatttta aatcaagtag tgaatataaa
    23101 aatttaagtg ctacaccaga tttgtcacct gtgttttgag attctggatt aactccttga
    23161 ccaaagataa ttatattctt ttccaatcta tcaagaagct cctttttagc ttcaactggt
    23221 atatttatct ctagtttatc aactccacct ccaccatcta ctttaattga tttatagtat
    23281 cttatattat ctataaactc ttgtagactt gttcctggat attcttttaa tacataaata
    23341 acctcttgta tttcatctaa gttatctgct agtgtagaaa tattattgtc atatatatct
    23401 attaatgatt tatagaaatt taaatctgat acgcactttt cattattttt aaaaggtata
    23461 aatggaactt taccccatcc ctgttctttg ttatttattc taaaatgacc ttcttgtata
    23521 tcagtcattt ttccatattc atcatataaa aattcttgaa taaaactatt acctctttca
    23581 ataaagtaag ttacgtcatt ttctgtgtag tactcaactc tttttatttt atttccatct
    23641 atatcttcaa tataataaaa cctaataaat gcaactaatt ccctctgtct tttactatcc
    23701 caaataggaa ttgcttcttc agcaggtatt attacatatt taaactcacc ttttctatta
    23761 atatatggat gtaaccattc aatacctttg ttactagcat tgaggtagag ttctgttatt
    23821 gtatcgtcaa attcttctcc tagtaagtca tttaaaagct tagtgagatt atcatcatct
    23881 gcattaaata ctatgggatt tccgacacta tagcctacct tttggtcaac taaaagctta
    23941 tggtagttgt taattgcttt attattaact ttagtaaaat catcaacctt agctccatct
    24001 aagagataat atcttctctt attgtttata tctgcattgc cataatagta ttcttcacct
    24061 tgtttatatt tctctggtct atgttttaaa atataatgct ctataacttt tactaggtta
    24121 aaggtgctct ctttttttaa ctgaactttt attaaatctg tttcacttat ataaatattt
    24181 aacgcctcct ttactttaag aagcttattc cattattttt aagcttatta tctatagaat
    24241 atctaagcgc agccattgca tcatccataa actcaactgg ttcatcaaga tataatccag
    24301 ttctttcatc ttgtttccat ttccattgtt gtatttcttt tatggtatta gtgcaactag
    24361 gatgtacatg tattcttaat tgtttcaaat aatctatttg agctttaaca cttcctggtc
    24421 cttttttaac tcctttagct tcatatcctg cactcttcca catcttaatt ctatctggtt
    24481 cagcactatc acagtacata aatagattct tttctaaacc tttattattt gcaatcttta
    24541 tgatttctga ggtatccatt tcatgtacat atatttcgtt acatatatat aattctccat
    24601 ccttaaagcc aattctaagt actacatttg catggttaaa tccaaagtct tgtgataacc
    24661 tcatattgtc aaaatactca aattctgtag gaaattcatg tataacataa tttttaagta
    24721 ttgctccacc agtttctccc cattctccaa gaccatagac tttgtaccct tctgggtctt
    24781 gctcttttct catttgcatt cttctgtagt aagcctcatc tatgaatcta ttttgtagat
    24841 aagtactatg atgagtaaat atatcatcat ttttatagtc aaaatacttt ctttttatcc
    24901 aatgagtagc tgagactgga ttaaatgtaa atgtcatttg atagtatagg ttaggattag
    24961 ttaaaatacc tcttaaacgg tcatctagta tgtctatgtc actttccata agttctgtag
    25021 cttcttcaca ccaaacccat gttaattttc ctttcgagaa gttaattgat tttaattttt
    25081 ctctttgttt tgcatcatta actcctctga aaattataga gttaccagta actttactct
    25141 taatttctaa aggatttaaa gtagttttcc aatacttatc agcttgttta ctataaatac
    25201 gatttatagc tcctgtaagc tctgcatacg ttgaatactt atgtgtagct tctgactttc
    25261 taactactaa tagattagct ccttgatact tcttatctcc taactttagt atatagtctt
    25321 gtgctacatt aacagatttt ccactccctg ctgaaccttt cattgctctg tatctttttt
    25381 tagtaaaatt agcttccttg aaatctggat taaaatttac tctaactatc atttctatca
    25441 ccataatcta cacttatttt caactcatca tctccaatat catctttact taggttatca
    25501 acttcacatt tcaacttctc aactcttgtt ttctgctcct ctgtagccaa attccaatcc
    25561 ttatgaatca tttcatcata ctgtttaatt aaacttctaa gttcactcat agctctactc
    25621 tgtgcattaa gaaaagatgc ttgcctatcc catgcaaatt gaaattcata ctctatcttt
    25681 tcaccatttt ctgtgctttc atatttcttt aattctttaa ccatttcttc cttgccttta
    25741 acatacatta tcttttgtgc tcttattatt gctgcatatt gaattgttat ctgttcccaa
    25801 agaatatcaa atttatcttt tatagatatt tcttgtatca attctctagt ttctttgggt
    25861 agatactttg agaagaaacc aaacttttca gcattcttat tctcttttgg agcaccatga
    25921 ccaactgaat ttttattaga aaagggtgca cctcttttat ttataggtgc acccttcttt
    25981 ttttcactag cccagttgta tctttttatc catgacttta aagtgtttaa actaatgtca
    26041 tactttgatg atatttcctt ttgtttcatt ccttttaagt aatcttgttt taccttttct
    26101 ttgacatctt gcacatcacc acctctttat ttgtttgttt tgggaataaa aaaacaatcc
    26161 tatttaatag gattgtttca aatctagtct cctctatcct tccaggataa atgaaacata
    26221 cttattatta atgtagttag agataaaatt atatgaactg taaaaaatat taaaacatag
    26281 aaaattatat tagaataata tcgagtaagc attattgtat tgataaaaca tattacaaca
    26341 accaaaatct ccattattac agtataatat gcttttaact gtatgaattg aaagtatgat
    26401 atattaaatc catttacctt tctttctgtt tttatatctt ttgcttctga tattgatttg
    26461 ttatatgtag taaacaataa tgtaataatg caaactccaa aagctactaa tagtcctgac
    26521 aaagttagta catctttgtt taaagaaatt acaacttctt tattaattga cgaattaaaa
    26581 aacatgtatg aaataataca tattaatact ggtgttaaaa ctaactctac tacttcttta
    26641 gaatttatag ccttgtaata attgagtaag atatttaata cctttttata cattttaaca
    26701 cctccttcgc aatctatgct tcactcatag caacttcttg atacattaaa ttaaaaaaaa
    26761 ctgaattatt ctcaattaaa tcatctatca aattattcat cttatcaaaa atatcttcac
    26821 tatctatatg attatctata tcaagagaaa cctctatttc tctacacaac tttataccct
    26881 cagtatccaa ctttaatttt ctcttgtttt tctctccctc aattattatc ctatttattt
    26941 ttttatttct aaattcctct cctttaatat atttttcgca acatctttta acttcactag
    27001 gtaaatatct agttctaaat gtgggcttat atatcaaatc gttatgtcgt cttgaattat
    27061 ttaaattaga aaacaaatta tcttcatcag ttccaaactt ctctctatca acatttaaag
    27121 ttatttttga aacaccatct aaatctaata tttgttctat aaaatcttta cttgctattt
    27181 gtgtaatatt aattgatgtt tttgacatat tattatcaaa tttattattt tcaatcatat
    27241 cctcataata tagatgtaag tttttactta gttcaatcat gggaaccccc acaaaaactc
    27301 tttcaaataa tactactgct atattatttt ccttcataaa tttaataaat aggtgctgct
    27361 tattgctatc tccttgatcc atttttttat taccctctac ttctttagta tgagcattta
    27421 taatatttgt attctgattg tatttagtat attcaagttt caccttgatt attaaatcat
    27481 cctcgctaca ttttacttct gtaatccaaa actttttatc tttagtaata ttaattccat
    27541 ttttgttttc tttacaatat tctttatcct taaagaaggt ctttattacc tcttttaaaa
    27601 tttcttcatc tgaatagaca ctcccattaa ttttatttat aatatctatt ctataaacat
    27661 caatatatct ttttttaaaa ttactcatta taccattatt cctttcacaa atatttataa
    27721 tgatataatt ctatataatc agtcaatatc cttcaataat cattcgacaa ccacaaaata
    27781 agtctaaata atacttttac cttattcctg tctataatgt acaaatatat ttcatttt
//
LOCUS       DAELTA010000038        27500 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.038, whole genome shotgun sequence.
ACCESSION   DAELTA010000038 DAELTA010000000
VERSION     DAELTA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.038"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..1810
                     /locus_tag="KPG13_001409"
     CDS             116..1810
                     /locus_tag="KPG13_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG0985145.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2142..2252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2356..2727
                     /locus_tag="KPG13_001410"
     CDS             2356..2727
                     /locus_tag="KPG13_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG0985146.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2822..3769
                     /gene="trxB"
                     /locus_tag="KPG13_001411"
     CDS             2822..3769
                     /gene="trxB"
                     /locus_tag="KPG13_001411"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0985147.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3873..4190
                     /locus_tag="KPG13_001412"
     CDS             3873..4190
                     /locus_tag="KPG13_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG0985148.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4459..5745
                     /locus_tag="KPG13_001413"
     CDS             4459..5745
                     /locus_tag="KPG13_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0985149.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5857..6330
                     /locus_tag="KPG13_001414"
     CDS             5857..6330
                     /locus_tag="KPG13_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5989..5991,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG0985150.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6362..7672
                     /gene="grdB"
                     /locus_tag="KPG13_001415"
     CDS             6362..7672
                     /gene="grdB"
                     /locus_tag="KPG13_001415"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7409..7411,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0985151.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7928..9460
                     /locus_tag="KPG13_001416"
     CDS             7928..9460
                     /locus_tag="KPG13_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG0985152.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9471..10616
                     /locus_tag="KPG13_001417"
     CDS             9471..10616
                     /locus_tag="KPG13_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG0985153.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10926..12005
                     /locus_tag="KPG13_001418"
     CDS             10926..12005
                     /locus_tag="KPG13_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0985154.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12118..12717
                     /locus_tag="KPG13_001419"
     CDS             12118..12717
                     /locus_tag="KPG13_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985155.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(12903..13775)
                     /locus_tag="KPG13_001420"
     CDS             complement(12903..13775)
                     /locus_tag="KPG13_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0985156.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14192..15595
                     /locus_tag="KPG13_001421"
     CDS             14192..15595
                     /locus_tag="KPG13_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985157.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15622..17166
                     /locus_tag="KPG13_001422"
     CDS             15622..17166
                     /locus_tag="KPG13_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0985158.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17197..18588
                     /locus_tag="KPG13_001423"
     CDS             17197..18588
                     /locus_tag="KPG13_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG0985159.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18781..20277
                     /gene="abfD"
                     /locus_tag="KPG13_001424"
     CDS             18781..20277
                     /gene="abfD"
                     /locus_tag="KPG13_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG0985160.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20412..20687
                     /locus_tag="KPG13_001425"
     CDS             20412..20687
                     /locus_tag="KPG13_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985161.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20711..22018
                     /locus_tag="KPG13_001426"
     CDS             20711..22018
                     /locus_tag="KPG13_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG0985162.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            22038..23156
                     /locus_tag="KPG13_001427"
     CDS             22038..23156
                     /locus_tag="KPG13_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0985163.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23443..24363
                     /locus_tag="KPG13_001428"
     CDS             23443..24363
                     /locus_tag="KPG13_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG0985164.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24478..25635
                     /locus_tag="KPG13_001429"
     CDS             24478..25635
                     /locus_tag="KPG13_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0985165.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25720..27219)
                     /locus_tag="KPG13_001430"
     CDS             complement(25720..27219)
                     /locus_tag="KPG13_001430"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG0985166.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT        10738 a         3130 c         5168 g         8464 t
ORIGIN      
        1 attataatat aaagaatatt tgatatatat aataatttat aaaagagtat tacatagtac
       61 atataaatct atatttataa atacaataaa gtaaagttgt aaaggagtgc tttaaatgaa
      121 gttttcagag tttaagtatg aaagacccaa ttataatagt atgaaaaaag agtttttgag
      181 ttgtgtagaa gatataaata attctcgtaa ttataaagag caacaaaaaa atatacataa
      241 gattaattta ttaagaaata aaatagaaac gttgtcaaat atagcttcga taagatatag
      301 tacagacaca tttaataaat tctacaaaga agaaaaaaat tattgggatg aatatatgcc
      361 tttgtatgaa gaattaaatt catattttta taatgccatt gtaaattcaa aatttaaata
      421 tgatttgata aaagagtttg gagagcaatt tttcacaata gtagagtatt ctttaaaaag
      481 tttttccaaa gaaataataa gtgaattgca ggaagaaaat aaattgtgtt cagagtatac
      541 tagactttta gcttcagctg agataatgtt tgatgggaaa ataagaaatt tatctggtat
      601 ggggaaattc atgtattcta agaatagaaa aacaagagaa ttagcaaaca aagcatatta
      661 taatttcttt gaagaaaatg aaactaaatt tgatgatata tttgataaat tggttaaact
      721 tagagataag atgtctaaaa aattaggttt tgaagatttt gtagagcttg ggtacattag
      781 aatgatgaga agtgactaca gagaatatat gataaaaaat gttagaaaac aagtactaaa
      841 atatgttgtt ccaatggcaa atgagcttta tgaaaaacaa gctaaaagaa ttggtctaga
      901 atatttaagt tacatagatg aaggcgtgga atttttaaca ggaaatgcaa gtttaaaggg
      961 agattcaaga tacataatta aaaatggtaa aaggatgtat tctgaactgt caaaagaaac
     1021 aaatgaattt tttgatttta tgttagaaaa tgaacttatg gatttagaaa cgaagaaagg
     1081 aaaaggagct ggagggtatt gtacttatat accagattat aagtcaccat tcattttttc
     1141 taattttaat caaactgctg atgatataga tgtattaaca catgaggcag gacatgcatt
     1201 tcagttgtat atgtctagat ggattgatat gccagaaatt aattttccaa cgcttgatag
     1261 ttgtgaaatc cattcaatga gtatggaatt tataacatgg ccgtggatgg atttattttt
     1321 taaagaagat acagataagt acaaattcac tcacttatca tcttcaatta aatttatacc
     1381 ttatggagta attgttgatg aatttcaaca ctatatttac aaaaatccta atgtagataa
     1441 gtcaaaacga aaagaaatct ggagattttt agagaaaaaa tacttacctc atagaaaata
     1501 tggtgataat tcttttttag agagaggttg ttggtggttt aaacaaggac atatatttaa
     1561 aaatccgttt tattatatag attatgtatt agctcaaatt tgtgcgttac aattttggaa
     1621 aaaaatgatt caagatagag atgcaggatg gaaagattat ataaatatct gtaaagttgg
     1681 aggaacaaaa tcatttttag atattgtgag tatgggaaat ttgtattctc catttgatga
     1741 tggctgtatt gagtctatta taggtgatgt taaatcttgg tttgatgaaa taaatgacag
     1801 caaattataa aaaattatga gaattttatt gtataaaagt aaaaaattaa aaaatatata
     1861 aaaagtgatt atttgttgtg aaaatagtct tgtttttaac taaattgata aaataaacta
     1921 aaaaagattt gtacttaaaa aaaaaagaaa atacaagaaa tatgcaatat caacatttgg
     1981 aaaaaaaagt aaaaatgtat acaaaataca tataaaaaaa ctattgcaaa ttttatagaa
     2041 tgtttatata cttaagtcaa cagagataga aaaaagctat ttatagaaaa caaaggataa
     2101 attgcttaat tttaaatttt ctgaaaaaaa taataatccg gatgaagagt atgggagact
     2161 gctttataaa aagcgaccga agggacaagt ttaatcttac ttacccaaaa gattagatga
     2221 aattctcagg taaaggtgac tgtacttgga cggggctctg aaaatgattt taacagggaa
     2281 gacaaattgg gagatttgtt tttcctgttt tttatttatc aagtaataaa taaaagtttg
     2341 aaagagagta aaaaaatgat aataattacg aataatccaa aagtaaaaga agaagttcaa
     2401 ggcagagaag ttttgtttaa agacacaact tatattggaa tcttagaagc aagtagagat
     2461 ttgatacatg aaggttatga acttttatct catccactct atgggagtgt aaagccaaat
     2521 gaaacacctt acaggactgt tatactaaag aaaggaaatc gtttggatat aaactcttta
     2581 accttaattg aagaagcaat catcactgca agtaaatttc agaataataa aaagactcca
     2641 aaatggacag aaagtgttca agatgatttt agagtaattg attatgatat attttataat
     2701 acaatacaaa gaatgcagta tgaataaaaa aattgttaaa aagttattaa aacagcctaa
     2761 atattaaatc taatttaata ttaaataaaa tgagaaattt aattggggag gataagtcaa
     2821 aatggaaaat gtgtatgact tagtaataat tggttcagga ccagcagggc ttgcagcagg
     2881 tctttatgga gcaagagcaa agttaaaaac tctaatccta gaaaaagaca agactggtgg
     2941 acaaattgta ataacacatg aaattgcaaa ttatccaggg tcagttccaa atgcaacagg
     3001 accaagttta atagctagaa tggttgaaca gtgtaaagag tttggagcag aaatgctaag
     3061 agacaacatt gttgatactg agctagatgg agatataaaa gttttaaaag gggaaaaggc
     3121 agaatataga gcaaaagcag taataatagg aacaggagca acaccaagaa agattggttg
     3181 tcctggagaa aaagaactta ctggtaaagg ggtttcatat tgtgcaactt gtgatgcaga
     3241 tttctttgaa gattttgaag tatttgttgt aggtggagga gacagtgcat tagaagaagc
     3301 aatgtattta actaaatttg ctagaaaagt aacaattgta catagaagac aaggatttag
     3361 atgtgctaag agtgttgaag aaaaagcaaa agctaatcca aagatagagt ttttattaga
     3421 tacagtaatt gaagaaatca aaggtgatgg aatattagaa tcagttgtat ttaaaaataa
     3481 agttactgga gaaactcatg aatactttgc agatgaagaa gatggaacaa tgggagtatt
     3541 tgtgttcgtt ggattagatg ctcaaactga tttattcaaa ggtaaagttg atatggatga
     3601 aaaaggttac ataataactg atgaagatat gagaactaac ataccaggcg tatttgcagc
     3661 aggagattgt agatctaaga ctctaagaca ggttgtaact gcaactaatg atggagcaat
     3721 tgcatctata gtagctgaga aatacattga tgaaaaattt gggaactaga atctgtaaat
     3781 taagaaatag tgtctgtaaa tttaatgaat aatatttaaa attaaaatta atatttaaaa
     3841 taaaaataaa aatttcaagg gggaataaga aaatgttaga tttagataag gctacatttg
     3901 aagaagaagt tttaaatgca gaaggttttg tatttgttga tttttggagt gaaggttgtg
     3961 agccttgtaa agctttaatg ccagatgttc ataaattagc tgagacttat ggggataaaa
     4021 taaaattctg taagatggat acaacaaaag ctagaagatt agctataaaa caaaaagtat
     4081 taggtcttcc aactatggct atatacaaag atggagaaaa agtagacgaa gttactaagg
     4141 atgatgctac agttcctaat atagaaaaca tgattaaaaa atatctttaa tatgttgata
     4201 aattattaac attaaaagaa aaaatctatt tttatctgaa gataagaaga aaaaggtttt
     4261 tatgtccaac tttgttaaaa aaataatttt aaataaaata atttttttac aataaatatt
     4321 ttttagtaat aaaataaaga tttaaggcat aaaaacacaa taatatatct ttaaaaaaat
     4381 aagataaatt gtaaaatgat ataattttga gaaaaactca aatgcatata cacaaattaa
     4441 ataacaggag gtgcaagtat gcgtcttgaa ttagggaaaa tctttataaa agatattcaa
     4501 tttggtgatg taacagaagt aaaagatgga gtattattca tcaataagca agagatgcta
     4561 caagaaattg gtggagatga gcatattaaa tctatagata tagaattagc tcgtccaggt
     4621 gaaagcgtaa gaataacacc tgtaaaagat gttattgagc caagagtaaa agttgaaggt
     4681 aatgggggaa tcttccctgg tatcatgtct aaagttgaca ctgtaggaga aggaaaaact
     4741 catgctttaa aaggtgtagc tgttgtaact actggaaaaa tagtaggttt ccaagaaggt
     4801 ataatagaca tgactggaga aggagctaag tacactccat tttctaaatt aaataatgta
     4861 gtagtaatag ctgaacctat tgatggttta aaacaatacg ctcatgaaaa agctgttaga
     4921 atgataggat tcaaagcagc aatgtactta ggagaagtag caagaaatct tactccagat
     4981 gaagtttcag tatatgaaac taaacctctt cttgaatcaa taaaagaata tccagagctt
     5041 ccaaaagtag gatatgttta catgcttcag actcaaggtt tattacatga tacttatgta
     5101 tatggagtag atgcaaaaca aatagtacca actttattat atccaacaga attaatggac
     5161 ggagcaattg taagtggtaa ctgtgtttct gcatgtgaca aaaacccaag ttatgttcat
     5221 ataaacaatg gggttgttga agacttatat gcaagacatg gtaaagatat aaactttgta
     5281 ggtgtaatta taactaatga aaatgtttat ctagcagata aagaaagatc ttcaaactgg
     5341 acttctaaat tatgtaaata tttaggatta gatgcagtta tagtttcaca agaaggtttt
     5401 ggaaatcctg atactgacct tataatgaac tgtaagaaga tagaaatgca aggtgttaaa
     5461 acagttatag ttacagatga atatgctgga cgtgatggag gttctcaatc acttgcagat
     5521 gcagatgtaa gagctgatgc tgttgtaaca ggtggaaatg ccaaccaagt tgtagttctt
     5581 cctaagttag ataaagttat tggacattta gaagtagtag atgtaatagc tggtggttct
     5641 gatggaagtt taagagctga tggaactatt gaagttgaaa tccaagctat aacaggtgca
     5701 actaatgaaa caggatttgg tcatttaact gctaaaggat attaagaagt attttattaa
     5761 acgattgagt aatgttaata aaacatatta tagataatta taataagtgt tatagatata
     5821 taaaaatata ttagaaatat aataaggagg attattatga gtttacttag taataaaaag
     5881 gttcttataa taggtgaccg tgatggtata ccaggacctg cgatagaaga atgtgtaaaa
     5941 acagtagaag gagcagaggt tgttttctca tctacagaat gctttgtctg aacagctgct
     6001 ggggctatgg acttagaaaa tcaaaacaga gttaaagatg ctgctgataa attcggagct
     6061 gaaaatgttg tgattttact aggtgctgct gaagccgaag ctgcaggtct tgcagccgaa
     6121 acagtaactg ctggagatcc aactttcgct ggaccacttg ctggagttgc cttaggatta
     6181 agtgtttacc acgttgttga ggaaccaata aaatcattat ttgatgaaag tgtatatgag
     6241 gaccaaataa gtatgatgga aatggtttta gaagttgaag aaatagaaga agaaatgtct
     6301 ggtataagag aagaattttg taaattttaa tgcctaaaag caaaaggggt gaaactaaga
     6361 gatgggaaaa ctaaaagcag tacattatat taatcagttc tttgctggta taggtggaga
     6421 agaaaaagct gatactaagc cacatgtggc agaaacttta cctccaataa gtttacaact
     6481 tgataaatta ttaggtgaag atattgaaat agttggaaca gtagtttgtg gagatagcta
     6541 tttcaatgaa aatatagata gtgcaagtga agaagtttta tcaatggtaa aaggttttga
     6601 accacaactt ttcatagctg gtcctgcatt taatgctggt agatatgggg ttgctgctgg
     6661 aacaataact aaagttgtaa aagatgcttt gaatatacca gctttaacag gtatgtatgt
     6721 tgaaaatcca ggagcagata tgttcaaaaa ggatgtttat gtagtagaaa cttctgattc
     6781 tgcagcaggt atgagaaagg cacttcctaa gatagctaaa cttgcagtaa aacttgctaa
     6841 tggagaagaa attggaactc ctaaagatga aggatacata gctagaggaa taagagttaa
     6901 ctacttccat gaagacagag gttctaagag agctgttgac atgttagtta agaaaataaa
     6961 aggtgaacca tttgaaactg aatacccaat gccaaacttt gatagagttg acccaagtaa
     7021 agctgtaaaa gatttatcaa aatgtaaaat agctttagtt acttctggtg gtatagttcc
     7081 aaaaggaaat ccagatagaa tagagtcttc ttcagcttct aaatatggaa cttattctat
     7141 agcaggagtt atggatttaa cagaagagac ttatgaaact gcacatggtg gatatgaccc
     7201 agtgtatgca aatttagatg cagatagagt attacctgtt gatgttctta gagatttaga
     7261 aaaagaaggt gtaattggaa aattacatga gactttctat acaactgtag gtaatggtac
     7321 atctgttgca aactctaaaa aatatgcttc tgaaattgga gcagcattag tggctgatgg
     7381 tgtagatgca gtaatattaa cttctacatg aggtacttgt actcgttgcg gtgcaacgat
     7441 ggtaaaagaa atagaaaaaa ctggacttcc agtagttcat atgtgtacag tagtacctat
     7501 ttcattaaca gtaggggcta atagaatagt tccaactata gcaatacctc atccacttgg
     7561 aaatccagcg cttgacccta cagaagaaaa agccctaaga agaggccttg tagaaaaagc
     7621 attaaatgct ttaactacag aagtagatgg tcaaacagta tttgaaaaat aaaaataaca
     7681 cacacatata ttatatagat aaaaatataa acacacacta aaaataaaaa ataactatac
     7741 acaaaaaaat aatatagatt tcatgaatgg ataaattgga gttattcatt catgaaatct
     7801 taaaaaatgc caaaatacat aaacaataat agaatcaaat atacaataaa atacaatgat
     7861 tagagctttt aataagttta ataagattaa aaaataagtg ttcataaaat ttatggaggt
     7921 gcacaaaatg acttatccag ttttaaaagg ggcaggctat gtacttattc atactccaga
     7981 tatgatagtg caaaatggaa gtacttgtac agttgaaaga gcaacaaatc cagattcaga
     8041 atttttaaaa gaagtaagta atcatataag aagttatgaa gatgtagtta actacatgcc
     8101 aaatcaagtt tatataggaa atagaagacc agaagaatta agagatttac caatgccatg
     8161 gtgtgaacaa aagattgagg gaactagaaa tggtaagttt ggagaaataa tgcctcaaga
     8221 tgagttcata gctttaatgc aaataagtga tgcatttgat ttagtaaaat tatcacaaga
     8281 atttattgat gaagtaaagc caaaaatcga aaataactac cctgaaattg caccatttgt
     8341 aggtaaatta aagggtgatg atatagaaga aggtaaagaa ttagtagcaa cacacatagc
     8401 tgaaggatta taccatgatg gaaaatttgt tggttatgta aaaagagcac atgatgtaga
     8461 tgttaactta aatgcccata caatgtttga aaatctagta gtaaaagcat ctggagtttt
     8521 atctgctatt caaatgctaa gacatagcaa aatagaccct gctgaaatag attatgttat
     8581 tgagtgttca gaagaagctt gtggagatat aaaccaaaga ggtggaggaa actttgctaa
     8641 atcaatagct gagatagcag gacttcaaaa tgcaactggt tctgacacaa gaggattttg
     8701 tgcagcacca actcatgcat taattcaagc agcagcttta gtaaaagctg gtatacataa
     8761 aaatgtaatg gtagtagcag gtggagcaag tgctaaactt ggaatgaatg ctaaagacca
     8821 tgttaaaaaa ggtcttcctg tacttgaaga tgttgttgga ggatttgcag tacttgtatc
     8881 tgaaaatgat ggtgtaaatc cagttataag aactgattta acaggtaagc atactgttgg
     8941 aactggttct tcaccacaag cagttatgac agcacttata acttcaggtc ttgatagagc
     9001 taatttaaaa ataacagatg ttgatgtgta ttcagttgaa atgcaaaacc cagatataac
     9061 taagccagca ggagcaggag atgttccaga agcaaactac aaaatgatag gggcacttgc
     9121 tgttaaacgt ggagacttag aaaagaaaga attgaaagat tttgtttcta acaaaggatt
     9181 accaggatgg gcaccaactc aaggacatat accatcagga gctccttata taggattttt
     9241 aatagatgat ttaactacag gaaatagaaa tagagctatg atagttggaa aaggtagttt
     9301 attcttagga agaatgacta acttatttga tggagtatct tttatagctg aaagaaatac
     9361 aggtgttact gaagagactt caggaatatc taaggatgaa attaagaaga taatagctga
     9421 atctatgaaa aaactagcat tagatatgtt agaagaatag gggtgaggat atgtcaaaaa
     9481 aagtaatcgc agatgtattc ttagaagtag ctaatgcaat agaaagcggc gaatttggca
     9541 aaaaagtaaa aattggtgtt actacacttg gaagtgaaca tggtgtagaa aacatggtta
     9601 atggtgcaca attagcaaaa tctaatttat ttgatattgt gcttattgga ccaaaagtgg
     9661 aaactgacct tgaagtagtt gaagttaatg atgaaaaaga aatgcatgca aaaatggaag
     9721 aattattaga ttcaggatat attgatgctt gtgttactat gcattataat tttccaatag
     9781 gtgtatctac agtaggtaga gtaataactc cagcaaaagg aaaagaaatg atacttgcaa
     9841 caacaacagg aacaagtgct actaatagaa tagaggcaat ggtaagaaat gccatttatg
     9901 gtattgctac agctaaatca atgggtaaca aatgcccaaa agttggaata cttaatgttg
     9961 atggagcaag acaagttgag aagtgcttaa aagaattaaa agataatgga tatgacatgg
    10021 agtttgcgga ttcaattaga gcagatggtg gatgtgtaat gagaggtaat gatttacttg
    10081 taggtgcacc tgatgttatg gttactgata cattatctgg aaatatattt atgaaagtat
    10141 tttcttcata tactacaggt ggagattatg aagctcaagg ctttggatac ggacctggag
    10201 ttggagagga ctatgataga aaggtactta tagtatcaag agcatcaggt tctccggtag
    10261 tagcaaatgc tttaaaatac gcttatgatg ttgtaaaggg agatataagt aatgttgcaa
    10321 gaaatgaatt tgcaaaggtt aaaaaagcaa aatttgatga cattatatcc tctcttacta
    10381 aaaaagaagt taaagctgaa aaagtagaag ttaaaatgcc agataaagaa attgttactc
    10441 gtcaaatagc tggtgttgat ataatggatt tagaagatgc agtatctgag ttatggaaaa
    10501 acggaatcta tgctgaaagt ggtatgggtt gtacaggtcc aatagtttta gtaaatgaag
    10561 ctaaaggaga cttagcagtt gaaactttag ttaaagctgg atatactgca aaataagtac
    10621 attttttata tgtaaataag aacatatagt tataatttta ttgattataa ttatatgttc
    10681 tttttatttt atatcttgtc aatatttata tattaacaca atccatttat ttgattctct
    10741 agctaattta ttatatttat tcagttaaaa ttaagctttc taactaaata aaaactaatg
    10801 aagtattaaa atgaaattga taaaatatga aaaaatttta ggaataaagt atctgttaga
    10861 taaaaataag attaattagt gatataatat aattaagata aaatttatta tttggaggca
    10921 aaatcatgag cagagtaaaa aatgtagtag aattgttgga aactaaaggt gttgatgcac
    10981 tatatctaac aaagaaaact aatgtaaact atataagtgg ttttcctgat gaagaagcat
    11041 atgcagtgat ttgcaaagat ggaaactttt tagttactga tagtagatat atggagcttg
    11101 cagaaaaggt ttgcaaagat tttgaaatta taaattggca taattttgat agaagtgtgg
    11161 caaaggcagt taaaagtgta tgtgataaag tgggtataaa gaaacttggg tttgaaagaa
    11221 ctaatatagt gtttgataaa tatgaagaac ttaaaaatct gattgagaag gataatggag
    11281 aattaatacc tacagaaaat attgtagaga ctttgagata tgtaaaagac aaagatgaaa
    11341 tcaaaaatac aagaaaagct tgtgaaatag cggataaggc attagaagaa ttgattccac
    11401 atataaaagc tggagttagc gaaattgaac tggctacaaa gttagagtac tttatgaaaa
    11461 tgaacggagc acaaaatata gggtttgaaa ctatactgat atcaggagca aagacttcat
    11521 tgcttcatgg taagcctagt gataagatta tagaaaaagg tgattttgta cttatagact
    11581 atggcgctat gtataatggc tatatatctg atacaactag aacttttata gttggaggag
    11641 cttctgaaaa gcaattagaa atatataatt tagttaagga agctcaaaat gtaggggttg
    11701 agaacatgaa agctggtgta catgcgacaa ttccagatgc tgagataaga aaagttgtca
    11761 aaaaatatga agactattac tatcaaggta taggacatgg agtcggtaga gatgttcatg
    11821 aagaaccttt cattggtaat tatggtgata aaatcataga agagggatgt ataataacaa
    11881 tggaaccagg tatttatttc cctggatggg gtggagttag aatagaagat actgttttga
    11941 ttactaaaaa tggtccagaa agacttacta aattccctaa agatttgatg attttagaca
    12001 aataatttaa tttatatata tttttttaca taaaatataa cataatctca ctattaagct
    12061 ctatttacat attatagtaa agagggctta attttattgt gagaggtgat agtatatatg
    12121 tataaaaaac ataaatttat aaaaaatata aaaaaagcct ggtataaaat caaattaatt
    12181 gatagatgcc ttataattgt aatggcaata ttaatgtttc aatctatata taatttattt
    12241 gttaatgaag taaattcaca agatactaca actattgatg tagtagtaag aaccacttca
    12301 gcagctatat ttgggtattt tttaagtgct aattttataa agagaccttt aaggaagtat
    12361 acaaatactg atgtatcttc aaatcttttt atagataata atttatcccc aaaaggtcaa
    12421 gagtctaatc aaaataatat aatgaatgtc aaaaatacca ttggatttac ttcagaagct
    12481 gataattatg aaaagaaaat acctattaat aataatgaag gttttgaaga aggagaaact
    12541 agtgaattgc aaattattat agctactgta atttgtatag tagcattaac agtacttttt
    12601 gttgtaagaa attttacaac tacaacatca gcttctttag gtactatttc acagatgaga
    12661 gattttgtct caggatgcgt gggttttttg ctaggttgtc caagtaaacc tgattaaaaa
    12721 agtattatca ataaaaggta ttttaaaaag tatcagtata ataatattta gatttaaatg
    12781 tatacaatta taattctaaa cattctatat tgtattttta tactgatact ctaaactata
    12841 ctatttatta aaatatttta acattgtatt taaacaaaat attatatctg tataatataa
    12901 atttagttta tatgaataga tgccatatac tcaataaaaa gtttcatact atgagatatg
    12961 tattgatttc ttctccaaag aaccccaata ttcgcagtaa ttgcacgttc taatggtatt
    13021 gaacagagat tttctgtatt ttgaaatatt tctttataca atattgttga agccaagtca
    13081 ttttctagta aataacgtat ggtagaaagt tgatttgagt gtaaaattat atttggctta
    13141 gctttttcaa gtctttttgt aataatctta tagtgataag aaccttcaga taagattaca
    13201 aaaggtacat tttttaacat ataattcgat ataatgggat gttttgccaa ttcatttttg
    13261 ggatttgtaa ctaagtataa ttctgtttta tagattgtct ttgaatctat atcatcataa
    13321 atatcactgt caattatacc aatacaaaaa tcaagtgtag attcttcaat cattttagct
    13381 ccaacaaatg ttggaacttc aaaaatttta agctctatat ctggataaat tacattaaat
    13441 ctaggaagta tttttgaaag caaaaacgta cctaaaactg taggaatacc tattttgaga
    13501 gaagcttttc tcttttttcc taaatcaagt gcgtcacatt gaaaatcctc aaattgtttt
    13561 ataaaatgtt ttgacttgtc taaaaaaatt tttccttcag gcgttaactc tattttatta
    13621 cctattctgt aaaaaagttc aaagccaagt tctttttcta attctttaat tgctaatgtt
    13681 atggatggtt gagaaatatg aatactttcc gcagccttag tcatgttttt atatttacag
    13741 acagtttgaa aatagtataa atagtttata ttcatttggt attagttcct ttcatttaca
    13801 tattagtgac aagtatataa ttaacaatag cataagtttt gtatattgtt gtaaaaaaat
    13861 agaatagttt taaactatca tagcataagt ttttgatatt ttcaatctat taagaatagt
    13921 gctattatta aattaacaaa agagaatagc tggtatggtt tagagtaaat caatctatat
    13981 tacagttata tcaacgaata tcaaaaaatt atctattaga aaataaaaat tatttttaca
    14041 attaaattgt taattaaatc ataaagaaaa aagtgatatt aacaagataa aattttttta
    14101 atcagaaatg ttaatttaat aacaaaatag agtattgggg agaaaggaga aatatatttt
    14161 ttaataatat ttgttaattt aataacgaaa aatgaaaggg agtgatttag tgaagtttga
    14221 attgatggat tttttaatga atccttttgt ccttatgttt gctgcagtaa ttacaggaat
    14281 tttatttgga aaaattaaat ttgggaagtt taattttggg gtatcaggag cattatttac
    14341 aggattattt attggatggc ttgcatatag cttggggaat ctaataatag caaaagggga
    14401 aacagcagca ggatataagg cagcaactgt aatgatgggt aatggaatta tttcttctga
    14461 cttctttgat ttctttttaa taatatttgt tgcagcagtt ggtctattgg cagcaaaaga
    14521 tatgaaagct gtattaaaga agtatggagc aagatttgta attttaggag ttcttataac
    14581 ttttataggt ggctctatga cttatgcaat gactttacta tcaagtgata aaggaagtag
    14641 tgcttatgaa gtttctggcg tttacacagg agcattaaca agttcgcctg gattagctgc
    14701 tgctttagaa acagctggta aacatgcaga agatgtatca aaagaatttg aaaaagcttc
    14761 aataaaagat aaaaaagaaa ttttaaaagt agtcgaccca gaaggcaaat tggatgtgaa
    14821 tacaactaca tcactgactc aagagcaaat agataagtat attgcatatg ctgaagctgg
    14881 tgttggtata ggtcatgcag tcgcatatcc ttttggagtt ttaatagtaa tcttaggtgt
    14941 aaatttcctt ccaaaattat ttaggatgga cctaaaagaa gaaagaagaa aatatgagaa
    15001 ggaaatgaag gaagcaagag atagtgtttc tggtaaaacg ataccagaag ttccatttaa
    15061 tataataaca tttttcttga catgtttagc tggatactta gttggaggaa tacatgtatt
    15121 tatgggacca ctagggtact tcacactagg cgcaacagga ggttcactaa ttgtatctct
    15181 agttcttgga tatataggta agataggggt agtaaatttc agaatggaag aaaaggtttt
    15241 aaatatttta aaacaaatag ggctagtatt tttcttagca atagttggat taagatatgg
    15301 tggaaaagta gttgattcaa ttatgacatc tggtatgcac ctagccttag tagcaatagc
    15361 agtaggtgtt acagctatga tgataggatt cttagttggc aaatacgtat ttaaattaaa
    15421 ttggatttta ttatctggag ctgtctgtgg tggtatgaca tcaacaccag gtcttggagc
    15481 tgctgttgat gctcttgata gtgatgaccc agcagcaggt tatggtgcta cgtatccatt
    15541 tgcattatta actaaagtta tattggttat agttttacac aaacttccaa tgtaaataag
    15601 attttatatg gggggatatt tatgaataat gaaggagcat ttgaagaatt aagtggaaga
    15661 ttaagaaata aagaattgtg tgatttaata atgaatgcag atgaagctgc taaaattata
    15721 gaggatggca tggttattgg agttagtgga tttacacctt caggctatcc aaaagcagta
    15781 ccattagcag tttcagaaag agctaaaagt ggagaaaaga taaaattaac cgtatattca
    15841 ggagcatctt taggtccaga agttgatgga gcatggtcag aagcaggtat aattgaaaga
    15901 agacttcctt atcaaactaa ttcaatactt agaaataata ttaataaagg tgttgttgat
    15961 tatatagata tgcacctaag tcattctact caatttttaa attatggaac tatacctaaa
    16021 gtagatgtag ctatagtaga agcacttgca ataactgaag aaggaaatat tataccaact
    16081 agtgggatag gtaattctcc atcttttata aaaagtgcag ataaagtaat agtagagata
    16141 aatcttgcaa aaccaatgga aatggaaggt atggcagata tatatattac ggaaaatcca
    16201 ccaaatagaa aaccaataga aataaatcat cctaaggata gaattggaac aacatatata
    16261 ccttgtggtt tggataaaat tgcaggagta gtaattacca atatgcaaga taagacccgt
    16321 ccattgggag ttgtagatga agcttcgaaa aaaatatcaa ataatattat tgctttttta
    16381 agagaagaag taaaggcagg cagattatca aagaatttac ttcctcttca atctggtgta
    16441 ggaagtgtag caaatgcagt attatatgga ttatgtgaat ctgaatttga aaatttaact
    16501 tgttatacag aggttgtaca ggactctatg ttggatttaa taagaatggg taaagttaca
    16561 atggcatcta ctacatctgt aagtccatct ccagaaggat taataaaatt tgaaaaagat
    16621 attgattttt ttaaggataa gattatatta agacctcaag aaattagtaa caatccagaa
    16681 atagccagaa gaattggtgt aattgcaatg aacactgcaa ttgaagttga tatttatgga
    16741 aatgtaaatt ctactcacat aatggggtca aagatgatga atggaattgg tggttctggt
    16801 gattttgcta gaaatggagc aataactata tttagtactg agtcaattgc aaaaaatggt
    16861 gatatatcat caatagtacc tatggtatct cacgtagacc acactgaaca tgatgtaatg
    16921 gtaattgtaa cagaacaagg atatgctgat ttaagaggtc ttgcacctag agaaagagct
    16981 ataaaaataa tagaaaattg tgcccatcca gattataaag aacaacttag ggattattta
    17041 aatagagctt gtaaaagtgg agcaaaacaa acacctcaca tattggacga agctttatca
    17101 tggcatagta aatttatgag tacaggtaca atgaaaaagg ctgaaacttt taaaagtgca
    17161 ttataaaata tttaataaat tggaggtaga ttttaaatgg aaaaagcagt ggaaaacttt
    17221 gaagatttaa gtaaggaata cattaatggt tatattgaga gagcaagaaa agctcaaaga
    17281 gagtttgaat gttatactca agaacaagta gataaaatag taaaaatagt tggaaaagta
    17341 gtgtattata atgctgaata tttagctaaa cttgctgtag aagagactgg aatgggagta
    17401 tatgaagata aggtagctaa aaataaaagc aaagcaaaag ttatatataa caatttaaaa
    17461 gataaaaaat cagttggtat aattgatata gatagagaaa ctggaataac aaaggtagct
    17521 aaaccagttg gagtagtagc ggcaataact ccttgtacaa atccaatcgt tacacctatg
    17581 agcaatgcaa tgtttgcact taagggaaga aatgcaatta ttatcacacc tcatcataaa
    17641 gcaattggat gtagtacaaa aactgtagag atgattaatg aagaattaga aaaaatagga
    17701 gctccagaaa atttaataca aatacttgac caacaatcaa gagaaaatac aagaaattta
    17761 atttcatcag cagatgtagt aattgccact ggaggaatgg gaatggtaaa agctgcgtac
    17821 tcaagtggaa aaccagcact tggtgtaggg gcaggaaatg ttcaatgtat catagataga
    17881 gatgtagaca ttaaagaagc agtacctaaa atcatagcag gaagaatttt tgataatggt
    17941 atcatttgtt caggtgaaca gtcagtaata gtagcagaag aaatgtttga taaaataatg
    18001 gatgaattta aaaataataa aggttttata gttagagata aagtacaaaa agaagctttc
    18061 agaaatgcta tgtttgtaaa taaatctatg aataaagatg cagtaggtca atcagttcat
    18121 acaattgcaa aaattgcagg tgttgagata ccagaagata caaaaataat agtaatagaa
    18181 gctgatggac caggagaaga ggatattatt gcaaaagaaa aaatgtgtcc agttatatct
    18241 gcatacaagt ataaaagttt tgaagaaggt gtagctattg caaaagcaaa tttaaatgta
    18301 gaaggaaaag gacatagtgt atctatacat tcaaatacag ttaaaaatat agaatatgca
    18361 ggagaaaata tagaagtatc aagatttgtt attaatcaat gttgtgctac tagtgctgga
    18421 ggaagtttct ttaatggtct tgcacctact aatactctag gttgtggttc ttggggaaat
    18481 aacagcatct ctgaaaatct tgattataag catctaatta atatttctag aattgcttat
    18541 tatatgccag aaaatgaagt ccctacagat gaagaacttt ggggataaaa tttagacaaa
    18601 aaatattcat tctaaattaa agtaggaata tattttaatt tagaatgaat ttaagacata
    18661 aattaaattt aaattagttt attggactaa atttaaaata ttaagagaca tatagaagaa
    18721 ggaagtatta taatttaaaa taaataaaaa aatctataaa agagaaggag tggttttatt
    18781 atggcattaa tgacaggagc tcaatatatt gaaagtttga gaaaattaaa tactaaggtt
    18841 tatatgtttg gtgaagaggt taagaattgg gttgaccatc ctatgattag accatctata
    18901 aattgtgtag cagcaactta tgacttagct catgacccag aatatgcaga tttaatgaca
    18961 gttacttcaa atatcacagg tgaaaaaata aatagatttg gtcatcttca tcaaagtgtt
    19021 gatgatttga taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac agcttcttgt
    19081 ttccaaagat gtgttggtat ggatgcgttt aatgcagtgt attcaacaac atttgagtgt
    19141 gataaagccc atggaactaa ttatcatgat aattttgtaa aatacttaac ttatatacaa
    19201 gaaaacgatt tagtagttga tggagctatg actgacccta agggagatag aagtctatca
    19261 cctagtgctc aacctgaccc agatatgttc cttcatatag tagaaagaag agaagatggg
    19321 ataattgtaa gaggagcaaa ggcacatcaa actggttcaa taaattcaca tgagcacctt
    19381 ataatgccta ctataagtat gactgaagca gataaagact acgctgtatc atttgcagtt
    19441 ccttcagatg ctgaaggagt gtttatgatt tatggaagac aatcttgtga cactagaaaa
    19501 ttagaagaag gtgcagatgt tgatttagga aataaggagt ttggaggtca agaagcttta
    19561 gtagtatttg ataatgtatt tgttccaaat gatagaattt tcttatgtgg agaatgggat
    19621 ttctctggta tgttagtaga gagatttgca ggataccata gacaaagtta tggtggatgt
    19681 aaagtaggag taggtgatgt aataatagga gcagcagctt tagcagctga ttataatgga
    19741 gcaaacaaag cttcacatat aaaagataaa ctaatagaaa tgactcacct taatgaaagc
    19801 ttatattgct gtggtatagc atgttcatca gaaggtcaca aaacagaagc tggaaactat
    19861 caaatagatt tacttcttgc aaatgtgtgt aaacaaaatg taactagatt tccttatgaa
    19921 atagtaagac ttgcagaaga tatagctgga gggcttatgg ttactatgcc ttcagaaaaa
    19981 gactttaaat cagaccttaa agttggaaca agtggaatga ctattggcga agtatgtaac
    20041 aagtatttta aagcttcttc agtggcttca acagaagaaa gaatgagaat attaagattc
    20101 ttagaaaata tatgccttgg ttcatctgct gttggttata gaactgagtc aatgcatggg
    20161 gcaggttcac cacaagctca aagaataatg atttcaagac aaggaaacat aaatcaaaag
    20221 aaagaattag ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa tttataaaaa
    20281 gttgatatat aaagggaaat tatatattaa tgtgtgttaa ttaatgaaaa aatatgtagt
    20341 ataagagata gttataaaat tatggtggtt tttagctaag taaagaaatt tttaataagg
    20401 agaatttaca catgacctgt ggagttagat tttgtggggg ctgtaaccct agatacgaaa
    20461 gagggaaggc tcttacaata atcaaagagc acttcaatgg tagggtagat tttagtatag
    20521 cacaagagaa cgtcgagtat gatttgttac ttataattgg aggatgcgca actctatgcg
    20581 catcctatca acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat tttagccata
    20641 tagaaagagt tataaatgtg attgaatcag caatttgtga aacttaaaaa agctaaatgg
    20701 ggggatttca ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg ctacagaagc
    20761 tgtaaaacag attaaaaacg gtgatactgt agtatttgca cattgtgtag gtgaacctcc
    20821 tgcactagta gaagctatga ttgaaaatgc tgaacaatat aaagatgttg agattaaaca
    20881 tatggtaagc ttgggtagtg gtggatatac tgctaaggga atggaagctc attttagagt
    20941 aaatccaatg tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag atggtgattt
    21001 tacacctgca ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat taaaatgtga
    21061 tgttgtttta gcacaggtaa ctccaccaga tgaacatggg tattgtagct taggaacatc
    21121 agttgattat acttatgaag ccattaaaac tgctagaaca gtaattgttc aagtgaatga
    21181 ccagtttcct agaacctatg gtgaggtagt ccatgtatca gagtttgact atatagtgga
    21241 aaaatcacaa cctttatttg aactacaacc tgcaaagatt ggagaagtag aagaagcaat
    21301 tggtaaaaat tgtgcttcat taattgaaga tggctctact ttacagctag ggattggtgg
    21361 gataccagat gcagttatgt tatttttaac tgataaaaag gatttaggga ttcattcaga
    21421 aatgatttca gatgggactt tggcccttta tgaaaaaggt gttataaatg gtaaatataa
    21481 aaattttgat aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa aattatatga
    21541 ctttgcaaat aataaccctg cagtagaggt aaaaccagta gactatgtga atcatccagc
    21601 aattataatg aaacaacata agatggtttc tataaattct gctatacaag ttgatttaat
    21661 gggacaggta gttgcagagg ctatgggact tagacaattt agtggtgttg gaggtcaagt
    21721 tgactttata cgtggagtgt ctatgggtga agatggaaag gcaattatag ctatgccttc
    21781 aataactaca aaaaaagatg gtacagtaat ttcaaaaatc gtatctattg tggatgaagg
    21841 tgcacctata acgacatcaa gaaatgatgt tgactatatt gtaacagaat acggtatagc
    21901 agaattaaaa ggaaaatctc ttagagaaag agcaagaaat ttaataaata tagctcatcc
    21961 aagtgtaaga gaacatctag cagtagaatt tgaaaagaga tttaaggaga aatattaata
    22021 atagaggggt gttaataatg gaagcactta gagtagttcc aaagatattt tactttgata
    22081 catttaaaga atttaatgaa gaatttaaaa taggaaaaaa tgatttagta attactaatg
    22141 agtttatata tgagccatat atgaaacctc ttggaataga tacaaattta atttttcaag
    22201 aaaagtttgg aacaggtgaa ccatctgatg aaatgataga ctccatgaca aaggaaatga
    22261 agaaatacaa ttttgataga attattgcat ttggaggagg aacaatagtt gatatatgta
    22321 agatattagc acttgatgtt ccagaaaagt ctattgattt atttgaagga gaagtatcac
    22381 ctaaaaaagt aaaagagtta gtagtagttc ctacaacatg tggtacaggt agtgaagtaa
    22441 caaatgtagc tattgcagag ttaaaatcta aacatactaa aaaggggctt gcagtagaag
    22501 aaacatatgc agactatgca gtgttaattc cagagacagt aaaagggtta ccttacaaat
    22561 tctttgttac aagttcggta gatgctctta tacatgctat tgagtcttat ctttctccaa
    22621 aagcaagtcc atttacagag atgtattctc tacaagcaat aaaaatgatt atggatgggt
    22681 ataagaagat agttgataaa ggagaagaag agagatttaa tcatcttaga gactttgtac
    22741 ttgcatcaaa ttatgcagga attgcttttg gaaatgctgg ttgtgcagca gttcatgcac
    22801 tatcttattc aataggtgga gctttccatg ttgcacatgg tgaagcaaat tatcagttct
    22861 ttactgaagt atttaagatg tattctagaa aaaaacctaa tggaaaaatt ataaaatgta
    22921 caaaaatttt agcagatgcc cttgaatgtg accctaactg tgatgtttat ggagaattag
    22981 aaaagttttt aaataaatta atagctaaaa aagctttgag agaatatgga atggtggaaa
    23041 gtcagataga tgaatttaca gatagtacaa tagcaaatca acaacgacta cttgctaata
    23101 attatgtaga gcttagtaga gaggaaataa gagagatatt tgcaaatctt tattaataca
    23161 attttttatt tatatgtata gtcaacataa acaatgctaa ttatatttat gtacaaataa
    23221 aaataaggga acttaaattt taattaaagt taagttctct tatttttata tggagttaaa
    23281 tataagtata tttttaattt aaaatatgct tatatttaag tctattactt tatttgaaac
    23341 aatttgtaga atttcaaatt ccttgacagt aataagtaag taattgtata atcaaactgc
    23401 aatatattaa taatattagt gaaaatgaaa ggattataaa atatgtataa atcagaaaat
    23461 ttttcaaaaa aatctgtatt aataaattca tttttaagta aatttttaaa aataattgga
    23521 tggggtttgg taagcttatt tacattatca gccttagtac tttcaactat ttccattata
    23581 attggatggg ctgtaatact tcctattcaa gtatgtgcag tatttttact tataggagga
    23641 gtttgtattt ttaaaggtaa gagattagga gaccaaattt ctcgttataa aaaatattgt
    23701 acaattataa acaatagaaa tattattcca gtagaactta ttgcagaaaa cacatctaaa
    23761 tcattaaatt ttataataaa agatgtacaa aagatgatag acaaaaacta ctttataaat
    23821 acttatattg ataaaagaaa taatcagatt gtgttaacaa atgaagattt tgttccacca
    23881 agatatgaag atgtagcata tgaagtcaaa gaagaagaaa agactgatga atccaatgaa
    23941 gttgatacaa ttataaataa aggaatgaat tatctaacac aaataaaaga agctaataaa
    24001 aatattaaat cagaatcaat gggcgataaa attgtacaag tagaagatgt tacaagtaaa
    24061 atatttgatg ttgtaaaaca tgacccatca aaacttactc agatacaaaa atttatggat
    24121 tattatcttc caaccacttt aaaactatta aattcatatc atacattgga agaacaagga
    24181 attgatagag aaaatataac aacaactatg gaaagtattg aaaatacaat gaatacgatt
    24241 gtagtagctt ttgaaaatca attggactat ctgtttgaag atgaagccat tgatatttct
    24301 acagatatta ctgttttaga gaatatgtta gtccaagaag gattaacgag tggtaaattt
    24361 taacttcaat agaaaaaata ttctgttatt tttaaacaat taattagtca taaagtataa
    24421 aatgtgtatt atataagtta atcagtactt tatgaaggaa gaggagagaa agatacaatg
    24481 aataatttag atgatatacc agttatgcca actttgacac ttgacccatt tggagaagaa
    24541 tcaaacacta atgatataga taattctgac ttattaatga aaaaggatga aaaagaccca
    24601 gaagaagaaa aactgtctga atctgaacgc aagatggtaa aggaatttgc ggaaaaaata
    24661 gatattacaa atacaaatat gattttgcaa tatggagctg gagctcaaaa aaaggtagca
    24721 agtttttctg aaacagcttt aaagagtgta aaaacaagag atttaggaga agttggagat
    24781 atgcttacaa atctagttac tgatttaaaa gcattttcag cagatgaaaa agaacaaagt
    24841 ggttttttag gaatttttaa aaaggcaaat aataaaattt caaatttaaa aactaagtat
    24901 gatagtgcag aggtaaatgt ggataaagta agcaaagagc ttcaaaagca tcaggttaaa
    24961 atattgaaag atatagcaat gcttgataag atgtatgaat tgaacttagc ttactcaaaa
    25021 gagcttacta tgtatattat agcaggaaaa caaaaattaa aggatatgaa agaatatgaa
    25081 atgccaaagt tgagagaaaa agctcgttta tctggttcaa cagaagatgc tcaatctgta
    25141 aatgatatgg tttcactttg tgacagattt gaaaagaaga ttcatgactt agaattaact
    25201 agaatggtat cgcttcaaat ggctcctcag ataagattgg ttcaaaatag taataatctg
    25261 atggcagaaa agattcaatc aactatagta aataccattc cattatggaa aaatcaaata
    25321 gtgcttgctc ttggaatttc tcacttaaat caagctatga aagcacagcg tgaagttagt
    25381 aatatgacaa atgagctttt attaaagaat gcagaaactt tgaagatggg aacaatagaa
    25441 acagcaaaag aatcagaaag aggaattgta gatatagaca caattaagaa aactaatcaa
    25501 tctctaatat ctactattga tgaggtagtt aaaatacaac atgatggtag aataaagaga
    25561 caagaagcag aagttgaatt atcaaaaata gaaaatgaac ttaaaagtaa gcttcttgaa
    25621 tttacagtta aataaaaatt atactaatat aaaaagagac tgtctcaaaa tagagataag
    25681 tctctttttc attgtttaaa tgattaaagt atatttatat tataaatttc tattatcttt
    25741 taacataaca tcatgagctc caccttcaac tatactagta gaagatacaa gtgtaagttt
    25801 agcatttttt tgaagttcag gaatgctaag tgctccacag ttacacatag tagaacgaac
    25861 tttacttaaa gaaagagtta cattgtcttt caaagaacca gcataaggca cataagagtc
    25921 aacaccttct tcaaaagaaa gtttcttatc tccaccaagg tcatatcttt gccagtttct
    25981 agcacgagca gaaccttctc cccaatactc tttcatataa ctaccattta tattaacttt
    26041 gttagttgga ctctcatcaa aacgagagaa gtatctacca agcattataa aatctgcacc
    26101 catagctaaa gctaaagtta tatgatggtc ataaacgata ccaccatcag agcaaattgg
    26161 aatataaata cctgtttttt caaagtattc gtctctagct tgagcaactt caattataga
    26221 agtagcttgt ccacgtccta tacctttttg ttcacgagtt atacatatag aaccaccacc
    26281 aataccaact ttaacgaagt cagcaccagc ttcagctagg taaaggaatc cttcgcggtc
    26341 aactacattt ccagctccta ccttaactgt atcaccatat ttttcacgaa tgaaatcaag
    26401 tgttattttt tgccactcag aaaaaccttc tgaagaatct atacataaaa catcagcacc
    26461 agcttcaact aaagcaggaa cacgttcagc aaagtctcta gtattaatac cagctccaac
    26521 tacatatctc ttagaagaat ctaaaagttc aagtggattt tctttatgag aagagtaatc
    26581 tttacggaat accatataca taagattacc attatcatca agtacaggaa gagaattaag
    26641 tttgtggtcc catataatat tgtttgcttc ctttaaagta acatctttgt tagcataaac
    26701 tatagatgac ataggagtca taaattcact aactttagta tctaaatcca tacgacttat
    26761 acgataatca cgacttgcaa caatacccaa taatttacca gttgaagttc catcttcagt
    26821 aacagctact gtagaatgtc cagttttttc ttttaagtca agtatgtctt ttaatgtgtt
    26881 atcaggttta atatttgaat cacttacaac aaaaccagct ttgtgggatt taacacgtgc
    26941 taccatagca gcttcatttt caatagtttg agaaccgtat ataaatgaaa taccaccttc
    27001 tttagcaagt gcaatagcca ttttgtcatc agaaacagat tgcataatag cagaagtcaa
    27061 aggtatattc atataaatat ctgcttcctc gcccttttta aatttagtaa caggtgtttt
    27121 taaacttaca ttagcaggta tacaatcagt agaagagaaa cctggtacaa gaaggtattc
    27181 actgaaagta tgtgatggtg tatcaaaata aaacgccatt ttttaatcac tccttatatt
    27241 tttatacttt ttataattga tagtattaat tataacttat tctttaattt tattaaagta
    27301 ttttgtcaaa tattgagatt tttgaaaaat ttaaactatt ttaattaaat ttgtttaata
    27361 tatattgcct tatagggtaa agttgtatat taaataatgt ttagtgttta atagtagcta
    27421 atttaaaatt aatctttagt gattcatata attacatttg tatagtttac attgataata
    27481 ttactagaga gtgcataaac
//
LOCUS       DAELTA010000039        27323 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.039, whole genome shotgun sequence.
ACCESSION   DAELTA010000039 DAELTA010000000
VERSION     DAELTA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.039"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(405..1691)
                     /locus_tag="KPG13_001431"
     CDS             complement(405..1691)
                     /locus_tag="KPG13_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0985167.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1814..4912)
                     /locus_tag="KPG13_001432"
     CDS             complement(1814..4912)
                     /locus_tag="KPG13_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0985168.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5638..5796
                     /locus_tag="KPG13_001433"
     CDS             5638..5796
                     /locus_tag="KPG13_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985169.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(6083..6787)
                     /locus_tag="KPG13_001434"
     CDS             complement(6083..6787)
                     /locus_tag="KPG13_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985170.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6778..8385)
                     /locus_tag="KPG13_001435"
     CDS             complement(6778..8385)
                     /locus_tag="KPG13_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG0985171.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8639..9817)
                     /locus_tag="KPG13_001436"
     CDS             complement(8639..9817)
                     /locus_tag="KPG13_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985172.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(10043..10537)
                     /locus_tag="KPG13_001437"
     CDS             complement(10043..10537)
                     /locus_tag="KPG13_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0985173.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10538..11299)
                     /locus_tag="KPG13_001438"
     CDS             complement(10538..11299)
                     /locus_tag="KPG13_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0985174.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            12001..14835
                     /locus_tag="KPG13_001439"
     CDS             12001..14835
                     /locus_tag="KPG13_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985175.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14878..16122
                     /locus_tag="KPG13_001440"
     CDS             14878..16122
                     /locus_tag="KPG13_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0985176.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(16537..18279)
                     /locus_tag="KPG13_001441"
     CDS             complement(16537..18279)
                     /locus_tag="KPG13_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985177.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(18263..18937)
                     /locus_tag="KPG13_001442"
     CDS             complement(18263..18937)
                     /locus_tag="KPG13_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985178.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(18974..19963)
                     /locus_tag="KPG13_001443"
     CDS             complement(18974..19963)
                     /locus_tag="KPG13_001443"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG0985179.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(20011..21255)
                     /locus_tag="KPG13_001444"
     CDS             complement(20011..21255)
                     /locus_tag="KPG13_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985180.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(21297..23843)
                     /locus_tag="KPG13_001445"
     CDS             complement(21297..23843)
                     /locus_tag="KPG13_001445"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985181.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(24051..24212)
                     /locus_tag="KPG13_001446"
     CDS             complement(24051..24212)
                     /locus_tag="KPG13_001446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985182.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(24268..25284)
                     /locus_tag="KPG13_001447"
     CDS             complement(24268..25284)
                     /locus_tag="KPG13_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985183.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(25281..26579)
                     /locus_tag="KPG13_001448"
     CDS             complement(25281..26579)
                     /locus_tag="KPG13_001448"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985184.1"
                     /translation="MIDSEEMIFNVEDLIIYRKNQLVIIYNKKFRKYIKLSEEIYKYF
                     LLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSNNLNFDMLSNGVAIYFC
                     LTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFF
                     EIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKG
                     VFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGR
                     GLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDV
                     YPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIF
                     CWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
     gene            complement(27045..>27323)
                     /locus_tag="KPG13_001449"
     CDS             complement(27045..>27323)
                     /locus_tag="KPG13_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0985185.1"
                     /translation="ACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKV
                     ITSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
BASE COUNT         9369 a         4451 c         2761 g        10742 t
ORIGIN      
        1 tagtttatca tatgattaaa ttataaatat cataatagaa ctctttaaat tataaaaata
       61 aaaacctatg tatcctcaac tttataagtt atatattaga gttgttttac ataggtttta
      121 gtatttttat ataataattt gttttaacat ttttcataat aagctttgat attttatata
      181 gtaatttgtt ctaacatttt tcataataag cttcaatatt ttgcataata atttattcta
      241 gcatttttaa taataggctc tagtatttta tattaataat ttattctagt attttacata
      301 attatttgtt ctaatttttt tcataataag ctttaatact ttatatagta atttgttgta
      361 gaattttata caatataatg atttatacag ttttataaaa tatcttaaaa cagtatttca
      421 gtaatatttt ttggtcttcc tccaccaata ctagtctctt ttgcacatat tcgacctaaa
      481 cctgcattta ctattttatt taatatacgt cttgcacttc tttcacttac atttagatac
      541 tctgctaact cttttgaatc ataaatctta ctttttctat tctcatttat agcaataatt
      601 tttgcaactg actcacaact cattccagtc tttttagata tttcgattac cttttcatca
      661 gatagcatca aagaataatt taattcattt tcggaagcta aaggtcctct tatgttttca
      721 tcttcatcta ccaaatatat accaatttct tttgaatcta tggagtgtct taatgcttta
      781 tgtgcattat tttcagcctg atacgcagtt actccagtac caattcctat actaagagaa
      841 aagcccatgt cttttatctc tctctttaga ttgaatagct tatcataatt atactcatta
      901 tcagctaccc ctttatgaac aaatacaaca taatcatttc ttccaagtaa aaataaggaa
      961 ccttgaatgc ttcttacata gtttactaca agcttatcca tatctgattt ttttatcatg
     1021 tcagaataat agttatctat cttctcttta taatctatta aactcaatat ttcaacagca
     1081 atttgggaaa atcttgcctt acttagagca tattcactct ttaccttgtc ataactttct
     1141 ttgattaatt gtatagtagc ttgtaaacga aatacaggat agcctctttc ttttaattca
     1201 ttatatactg caccaaaacc agtcaacatt atatccgtct tattttcttc aaataatttt
     1261 atatgccatt ctacaagttc agactctcct acttccaaag aaaatggatt acaaaataca
     1321 ctcgttgctt taatatcaaa ttctttaagt gcatcttcca caacatatct atcaactaca
     1381 tctatactga atttatctaa ttttatattt gcacttttta ttgcccaaaa tgccttcatt
     1441 atactcgtcc cacttctcgg tacaaatgaa tgtggcttac ttatctcata cttagatttt
     1501 ataaattcat aaactgcaca tcctgtaaat ataatagcat cacattcgtc ttcacattta
     1561 gaaataactt ctattgtatc tactactttt tctcttacat atattttagt ctttaaatca
     1621 ctgtctatct gttttaaatc atcctttatc ttaaacccag agtctgaagg acctataata
     1681 cccactatca taaacttcgc tcctaatcca taaatattat actttttaaa aattatatcc
     1741 tcactatttt ctacttctat ttaattattt tgaactactc catcctacct acatttagaa
     1801 tggaataatt agcttatttt tgataaagta acttctcaaa atcagcactt ggaataggct
     1861 tacaataata ataaccttga attaccacac aatctaaagt cactaaaaat tcagcttgtt
     1921 cttttgtctc tacaccttct gccactacat ccatatctaa atcttttgcc atggctatag
     1981 tgtgctttaa aactattttt tctctgtctg tgattccttc ctcattaaag aaaccacaat
     2041 ctatctttaa tgtatcaata ttaatatttt ttaacatatt taatgaagaa tatgctgaac
     2101 caaagtcatc catatttaaa acaataccat ccattctaag gtcggctatt atcttagtaa
     2161 ataattttat atttttaata aataaacttt ctgtaagctc taattctaaa aaagattttg
     2221 gtatactata tttatttatt aaacttaaaa caatttcttt aaaatgtgga ttatgcaaat
     2281 gtaatcttga tacatttaca gagataggaa ctggctcttc acctaaatct atccattttc
     2341 taatcaaaga acatacttgt tcccatacat acatatctaa tctgataata aacccattct
     2401 tctcaaatag tgaaataaat ttaattggag gtattatacc tttttctgga tgaacccatc
     2461 taaccaatgc ttctgcacca acaattttat ttgcattaag attatactta ggttgtaaat
     2521 atacatggaa ttgcccattt tcaagtgcgt attccatttc cctttccatc tgtttttctt
     2581 caagtgcatc atttcttata gtatcatcat aaaatatata gttattgata ctttgtttct
     2641 ttactttttt gtgagcatat cctgccattt cacacgcatt tgtaattgga atatgattat
     2701 ccaaaattct ataaacccca aaacatggta ctaacatata ttcaaatttt tttctattta
     2761 agctattttt aatcttataa attaccttat gaatatctct atcagagtaa ctctcataaa
     2821 aaatgcaaaa tacatctgca tacatacgac aacatatacc attagaacca atgttttttt
     2881 gaatcaactc tgcaacatat attaaagctt tatctcctcc tctaactcca taaatgtcat
     2941 taattacctt gaacttatct atatccatca ttataattgc ataacgcttg tcaggatatc
     3001 ttttaagcat taaacttatt tgttcacaaa aactttcata attgtatagt cctgttaatg
     3061 tatctttttg gtttttatgt agttgcataa gataatctat attttttata gcaactaaaa
     3121 tatgtgtttg tttctcacct aacttatttt tactactaat ctttaacata taaattaatg
     3181 cccaatagta ctttttatct attcctaaac gtctatactc aaaatttagt tctaaatcgt
     3241 cttgcttgct attattaaga ctatcaagag attttagaag gtggttatca ttaaataata
     3301 ttatatcatc aggatgaatt aaatgttttt ttatgtatga aaaagttttt tcaagatttt
     3361 cctttatagg aaaactaaaa atattgctct ctgattgata aattggagta attaaattcg
     3421 tatcaatgca aatagataaa attatatcat atgtattata agctaatttt tcataagcct
     3481 tagaataccc ttttatcatt aaatcatcaa aataaacctt atcaactaac tctttatgtt
     3541 taaataaaat attattcgtc gaatcagcta agtcactaat tactttcatt tcattatttc
     3601 taagtttgtt cttattataa tacctcttct tatcttggta cattctctca tcagcaactt
     3661 taattagctt atgtatatca atattatcgt cactccaaat actgcccact gctacactat
     3721 atatcatctc atccttaaat tcttcactta accaactcac taattcttca aatagttgtt
     3781 ttgatatatc ttcacataat acaacaaact catctcctcc aatacggtaa acattataag
     3841 aatgaaagta tttttttatt attacacatg ctgacacaat agcagaatcg ccattagcat
     3901 gtccaaagtt atcattaata tcttttaacc catttatatc tacaaataca actcctatgg
     3961 atttaacttt tcctttttga aaattatcta tatacttaat atatttattt ctattatatg
     4021 caccagtcaa gaagtcatag tagctcatat aatttaattt attttctact tttctttttt
     4081 tcatctcagt taataaaaaa taagataggg atgtcaaaag agaggtatct ccaacagctt
     4141 tatgtggatt atcaactcca ataaacccta ctactttatc atcttgtttt agtgaagatg
     4201 tcattaaact ttcaattttt tgaggtttta aaatttcata ttctattggc tgttcttctt
     4261 ttatattttc tatatcatca attattacaa aacctttttg ttcgaataat aaaaaccaat
     4321 tctttaattc attaatatca agatttgtta gtgcctgttt ttgttcatca acaccttcat
     4381 tgcaccattc atatgtatta gttacttttt ttctatcttc tgatacttca aaaacatatg
     4441 ctctatcagc tttatgaaat ttaccaatat tggttaatac aagatttaca gcatcagaaa
     4501 aattagattc tgttataaga tgtttaatac atccaactaa taactccttt atctctaatt
     4561 tttctgctat tgacttactt atatcttcct tttctgtaat atcaagagct atttctaatc
     4621 gtgcatgttt gccctcccat ttaattaact tgtcctttaa tataaaatgt ctatttgttc
     4681 gagtattcgt attttcccat atgtaatatt catcttcttt taaaatagag tttgtacaaa
     4741 attcgcaggg agaatctttc ccctgtaatg ctttataaca ttttaaatgg gatatatcat
     4801 taagtcccat tgcctttttc ccaatttcat taatatataa aagttcatat gtatctaaag
     4861 aagaaacata tacaatttca tccaactcat ctattaaatt tattacatcc atttatatat
     4921 atctcctttt attcattttc cagattgaat ttttaaagct tttaaaattt tgctatatat
     4981 ttggcaatga tattatattt ctttatgttt taaaatagta ccttaatgag gttctatttt
     5041 ttagtaaata gaattattat tgtgtcaatc gtacaataga gttttgtaat acctaattta
     5101 atatatttat aattaattat ctcattatgt gtttataatt aattctcata taaatgacaa
     5161 atctccacct caattaagta ttccaatcat atatgaagat aatacttcat tcatttaaaa
     5221 atataaaact attatatatt aaaatttctt caataataaa ttatctagat tgttactttg
     5281 atatattata taatatcaca aaggctgata taaaaagaca aaaagggcaa tttttttata
     5341 tcaaaaaaaa atgttaaatt gatattatcc atatataaat tgatattatc catatagatg
     5401 ctatatttaa caattctact tacttccctt acacttatta gcatatagtt attaagagtt
     5461 tgttgcaaaa caacacttag cacactttaa aaagtgtggt ctaataagta atcaacgaag
     5521 aagacataat aaagcataaa atatgatata cttttccacg ccatcaaaac tgaggatttt
     5581 attattaata tatgctaaaa tatgcttaat aaattaaatt ttaatgggag aatagttatg
     5641 aaaatattta gtttcttgtt gtttgtgata ggaatatgtt tatgtattgc tggatttata
     5701 ggacattacc cagtatttgt tggtacaaca ttgagttttc ttgcaattgc aatgatagct
     5761 gtaagacaag tcaaaactaa gaaaaaaatg aaataggcaa atcctaactt tacaagttaa
     5821 tattacacaa gtaattagtc taaaattgga tactttttct attacagaaa tacatcatcc
     5881 actttgtatt ttcttaaaaa aataaaatag ctatgtaaat tcaagattag atagattaac
     5941 tctctatcca tttcattgta aaatatacat agctatttgt attcgattta aaatacttag
     6001 attttcttac ataacaaata atttctatct gattattact catattcatt tagtaaccac
     6061 tcagcaacta atcatttagt atctattcat ccgccatacg gtaacctacc ccaacctcag
     6121 tcaaaatata ttctggctga gcaggattct tttcaatttt ccttcttata cttgccataa
     6181 aaactctaag cgactgattt tcactttcat agtaatttcc ccaaacttta tctattataa
     6241 acttatgggt taagactctt cctgaatact tagacatcaa ctcaataatc ttatattcga
     6301 ttggagttaa atgtatttct tcaccattaa ttgacacaat atgattttca taatctatct
     6361 ttaggttttt aacttcaaaa gactttggag catcattatt tatattatcc tgttgagctt
     6421 tatttctaag agccacacgt actctagcca aaagctctgt tacactaaaa ggtttagtta
     6481 aataatcgtc tgctcctcca tctaaagctt ccaccttatc cctctcatgc tctctcgctg
     6541 aaacaacaat tattttatta gtctttgcac atgccctaac tcgtcctatt acctcaatcc
     6601 catcaatatc tggtagtcct aaatccaaaa ttatcaaatc tgggtcaaga gaatgtataa
     6661 gtgatatagc tgttcctcca ttatctgtct ctacacacct ataattttga gtttcaagag
     6721 atacttttat aaatttacaa attggcttat catcttctac aattagaact aatggcttta
     6781 tttccacttg tctttcccct cctcatcata cataggtatg ctaaatctaa atgtagctcc
     6841 tccagaagga ttattaaagg cgacaatttc tccaccatga gcttctacta tagatttaca
     6901 tatctccaat ccaagaccaa tacctcttct ttcaagattt ctagacttat tcttagtata
     6961 aaacctatta aaaatattcc cgatatcttc atttggtatt ccttttccat tatctgcaac
     7021 ttcaaatagt acttgatttg actctttttt tacagttagt tctatcttag aatttttagg
     7081 tgtatgcctt atagcattat caattagatt tactaacact tgttctatta ataatacatc
     7141 cacatgaacg attattatct catctggaat atttgttttt atgtctctac tattggcaaa
     7201 ctttttgact cttaatatag attcagctat tatttcatca acaacttctg gtcgtttttc
     7261 aatatccaat tttccttcat ctattctggt catactaagt atattctcaa cagaatgagt
     7321 tagccaactc gtatcttcat atatattttt caataattct actctagttt ctttatctat
     7381 cacatcatca ttttcaagta ttgttgaaga tgaacctaat atacttgtaa gtggagttct
     7441 taaatcatga gaaattgacc taagcaagtt tcctcttaat ctctctctct ctgcctcaag
     7501 atttgctttt ttatttttct caaataaatt ttctctatca attgccaacg caacctgtgt
     7561 tgaaactgct tctaatagta tcttttcatt ttctgtaata atatctatct taaaacacgc
     7621 aacacctata acaccaagaa catttgattc ttgacctttt atagggaaat aatatgcact
     7681 gctgttttta caatattttg tcccaattcc aactgggtta cctgtcctaa aagcttcttt
     7741 tatagcttct ttttctaata ctgaatggaa tatattctca cttccatcat tgttacaaat
     7801 ataaatactt ggcttaccta gattattatt ctcatctgca atagtcacta ttacagttct
     7861 atttaatata tttactaaat tagacccaca aaattcaata atttgatttt tattttttgc
     7921 tttaagcaac gttttgttat tctcatagag caatcttatc attttttctc tcttatgaga
     7981 aagtctcact tctcttttta tcctagaagt taaagtactt gttataattg ctgccattag
     8041 cataatacaa aaggtcatag gataatcttt tctataagct aaaaaagtat ataatggatg
     8101 cgtaaagaaa aaattaaatg atagcactcc tattactgaa gaaagtattc caaaaaaata
     8161 tccataggtt atacttgtag caaatagtac acctaatatg tatactagta taatattaga
     8221 ttcattaaaa cctacctttt caaaaactac tgatattcca gtagcaattg tcataaccaa
     8281 tatagttttt atgaagtcct taataattat ctttttactg agatagccat cttcatcata
     8341 atcatactct tttaagtctt catgttgtag tcgttccttt ttcatttact acaattcctc
     8401 cttattgtta ctgtttaaat tggctctatt ttatataata caattttatc ataataaatt
     8461 caatcaatac atttaattaa tgtacttaaa ttactaaaag gtgctgtctt aaaatgaaaa
     8521 taaatctcta tttcaaaaca gcacctatat atttaaatta ctaatttagt ttttacatac
     8581 ttaaatcaat agttttattc aatttcataa attataatac ttcaattata aaacatttct
     8641 atccaacaag cataaatata aagtgggcac atatacctgc acataaacca cctatagtat
     8701 gcgaaagtat cgctttacca gccaatggtc ttgcatctaa agcatccatc atacctatgt
     8761 gtgtacttaa atatccactc caacacatac ccattgctgt gaatactgct atatcattag
     8821 gagttatagc gcctgatttt ataaactctg gtactaaaga tattgctgct ccaacagctc
     8881 ctaatgcagt tacagggaat gctattgcct ctggtgatgt gaatccaaat aatggttcta
     8941 ttatgaagct aatcttatca ccaatagcag gtaataattt tataccttca tatgcagctc
     9001 ctgtgtaaac agcttgacct gtagcagggt ctgtagatgg tccaaaagtt aataacataa
     9061 ctaatgtaca aactactaaa actcctggta ttatagccat acccatttca acacctaact
     9121 taccaccttc aagtatagca tccaatgttc tttggaatac attaccatct cttatttctc
     9181 taaactcttc aagttttgca cctttatcat ttatttgctt ttcagtatca tttttagggt
     9241 cataattata atacttttta gtaaaagtca acataattct aacactaata atactaccta
     9301 ttattgccgc aacattacct attatagctg gtagtacata ttctttacct tgagctatca
     9361 taaaagttgt tacaataagt cccataccaa aggctgttcc taaattacat aaagcaggta
     9421 cttgatactt cttaaaatat tgtgtaaatg ttttgtcttt agcaaatggt ataatagctg
     9481 gattatcaga taaatatgtt gcaactactc ctgctatact agcaccaggt aagccccata
     9541 taggtctcat aagaccctta aatatcttat taactaaaga tattactcca aactctgaaa
     9601 gcaatgcact aagtgcacct gcaagaactg ccatagccat tattaaaaat actgtgtcta
     9661 ataaaagcgc atgtgctgta ctcattatta ctttaaacat cattcctgca cccatgatgc
     9721 ttcctacata tccaaatcct accgccaata gtacaataaa tacgaaggtt tccatactta
     9781 ctgccttgac tcttctctta tccgacagct tttccatctt gaaatcttcc cccttttaat
     9841 tttaaattaa ggttcctaac attggtacaa attttttggt acaaactgag ttctaaaact
     9901 tacactcgta atgcaagttg tcagtttttt gaaataaaaa aaaattacgt ttatagaata
     9961 agaatatcct ctttaatttg gatactctta ttctatttta tgttattata ttatcatcct
    10021 tttgtttaaa agtcaatttt ttctattttt gttgtttaat tttatttata ctctttttat
    10081 atttttcatt ttcttttatc tttgtaagta ctccatataa gttgactttt ctaaatacag
    10141 tagttataaa taccaatcct aatgcaattg aacttgcaca cgataatgta ctcaccccat
    10201 attctacagc ttttatataa ttattggtca aaacagaata catagtatta tatattccat
    10261 aacctggtac taatattatt aaacagcaaa cacttaatgt agttgctgga gtcatataaa
    10321 ttcttgcaca taactcacaa tatatcgcaa aacaaactgt agctataaaa aaacataagt
    10381 tttctgttac tcctatattt aaaagaaatt tatatgaaaa ccaagctata cttccaccta
    10441 ttcctgctag tattaagttt tttcctttca tattaaatag cactccaaag cttaaggcag
    10501 taaaaaaagc tgctattact tctataaaca aatgcactta tatcccccct aacttgaagt
    10561 acaatccaag taccaagaac attcctactg ctatagatac acctacaaaa aatacttctg
    10621 ctaattttgc aagaccagaa atcaactgtc catctaacaa atctcttaat gcattagtca
    10681 aagcaagccc aggaactaag agcattattg ctcctgctat taaatggtct aacgtcttta
    10741 tgaatccgag ctttaaaatt aaaattgaac aagaagctat aataactgct ccaattgcat
    10801 ttacaaaaaa gtcattaagg gaagatttac ttaaatacat aataacaaac ttagtcagaa
    10861 tcccaacagt aaatgcagct ataaattctt ccacatttcc tccaaataat atcgaaaaag
    10921 ttgctgctgc aatacctgca aaaaatattg ttattaaatc tgagtaatag ttatcttcac
    10981 ttatttcatc taaaattttc tcacataatt caatatctaa attatttata cttattgctc
    11041 ttgataatga gttaacttca tgaaccaagt ttaggtctac tcttcttgag cttattcttt
    11101 taattgctga atgtaatttc ccatctacaa atacagatgc cattacagct gttggagttg
    11161 caaatacatc tacgtggtct attccaaaag attgacatat tctagctatc gtttcttcaa
    11221 ctctataggt ttctcctcca ctttgcaaca tagctttacc tgcatttgag gaaaagttta
    11281 aaattctatc tatatccaca aaacatatct cccattcttt tatagtatta ttcatatttt
    11341 aatacaagat attatttatt taaactattt taaataattc atatccaata tttacatttt
    11401 atgaaaaaac ttgtttttta tgcttgtcaa aatcaaattt taagcttgtt tacaccataa
    11461 aatggctgaa ttaatatttg acaaacactt tcaaccattt atataacctt ctaaattgta
    11521 taacttttta ctacaaataa aattatatct cttttaattc tttctcttga gtatttagct
    11581 tatagtaact tcatatttat atatgtacaa tataaaacat tatttttatg atacaaaata
    11641 taatttgtaa atgtatatat gtattaactg tgtatatata aattattctt gaccttatca
    11701 gttttttcat atatcttcat ctataccaac catatattaa taaatgatat tacatagact
    11761 taagtatatt aaaaaactta aatttagtta tttaacaaca taatactcta atttaatcta
    11821 taaaaatcca tacttaatat aatgcaactc ttattttaac accatctatt acacaaaaca
    11881 acatattaaa aagtaaaata caaaatatcc actataaacc ttattttaat actgaatata
    11941 tattataata tatttaatgt agcttaaata tagtttaaac ataaagaaag gaaacggtat
    12001 atgctaagaa aagataaagt tctattatct ctacataaat tatgtgaaaa tatgacttta
    12061 aaagaccttc aatcagataa aacaggattc actacaatag aaattgctaa ccattcatct
    12121 cttgatagaa gtaataccag taaagaatta aataacctat acaatgaaaa aaaagttctg
    12181 aaaatatctg gtaagccaat tttatttcta catgctagta ttatagaaaa tttaattggc
    12241 cgtactttga acagtgatga attttctata aataattgta atgagctact taaatcaagt
    12301 aaaaatgact cagaagatat tttttcaaca ttacttggac atgattctag tcttaaacta
    12361 cctatagaaa aagctaaatc tgctatactc tatcctccaa aaggtcttaa tacgttacta
    12421 ataggaccta ctggtgttgg aaaaagtaca tttgctgaaa caatgtacaa atatgctgta
    12481 gaaagtaaaa tcttttcttc tgaaagcaaa ttcgtagtat ttaactgtgc tgaatatgca
    12541 gaaaatgcta acttattatt aagtaattta tttggttatg tcaaaggttc ttttactggt
    12601 gctaataagg ataaattcgg catagttgca caggctgatg gaggcatatt attcttagat
    12661 gaaatacaca gattgccacc agaaggtcaa gagatgcttt ttcttttgat ggacaaaggc
    12721 atatacagac gattaggaga atctgacatt atgcacaaag ctaatgtctt tattgtgtgt
    12781 gccacaacag aagatataca ttcttctctg cttgggactt ttttaagaag aatccctgtt
    12841 acaatcaacc tacctgcttt acaggatagg tctttaaaag aaagattatc tcttattaaa
    12901 tacttcttta ttatagaatc aaaatacacc aatgcaacta taaaagttca caaagatgta
    12961 attaaatcat tactccttta tgattgtttt ggtaatatag gtcagcttaa atctgacata
    13021 caacttatga gtgcaagagc cttcttaaat tacaaaactg gacttaagga taatatagaa
    13081 atagatttat cccttgttcc tgattacatc tatattggtc ttttaaaatt gaatgaaaat
    13141 cgtagtaatc taaataaatt atcagaactt gattatatgc ttttttatga atttaatggt
    13201 aataacttag aagttgaata tacaaaagaa ctatctaaat acaatataga tacatcaaac
    13261 aatggattta aatactctac agagattcct tatctggaaa atgatataag gagtatgttt
    13321 ataaattaca tggaaaagta ctctacaaaa ttactttcga tatcttcagt aactcattct
    13381 gattatacaa gtaatgagat ttttaaagta atcaatccaa agatttacta tgctgttgaa
    13441 cattctttaa aagaggctga aaaaatatcg agaaacaaat acacaaaaca aacttatatt
    13501 gctctttcaa tgcatataag tgcacttata gaaaatataa atcaagacac atataatcct
    13561 aagtacaatt tatataatga taaaattttc ttagataaag atttaaatat agctaaaata
    13621 gttttaagta taattgaaaa tgacttacaa acatctttac ctaaagatga agtaaaattt
    13681 atatccatgt ttctaaatag catgtatctt gaaccaaaca atcaatctga aagtatagga
    13741 gtcataatac ttgctcatgg aaattcaaca gcaagcagta tgtgtgatgt agcaaattca
    13801 ttgcttggta caaaccattg taatgcaatt gacatgcctc ttgatgtaaa agtaagtacc
    13861 atacttgata aaactataga gttggtaaaa gaatgtgacg aaggtaaggg tgtacttctt
    13921 ttaacagata tgggctctct tatagctttt ggagaactta tttcagaaaa aacaaaaatc
    13981 aaaactcgct caattgaaat ggtatctact ccaatagttt tagaagcagt aagaaaaagt
    14041 acactaccag aaatgacttt ggatgaatta gctaattcct taagctcttt aaatccatat
    14101 attggacgag cagcaatcga ttcaacagaa ataaactatc ctgaaaagtc aagagagtat
    14161 gtaataataa ctacttgtat aacaggatat ggttcagcta tgaaagtagc agactttata
    14221 aattcttcac ttaaaaatat tagtaaatac aatattaatt tagttcctca taatatagaa
    14281 agttttaaaa tgtataagaa taaatttagt aatactaaaa ttctggcagt tgttggaagt
    14341 gtagatttta atgtagccaa cacccctttt atacacatag aggaactact aaatggatat
    14401 ggcctagata tgctaaacaa aataatagaa ggtacattca atgccaatag tattacaaat
    14461 ataaaagaaa caaatctatc atacaacagt ttaataataa actttatgac ccaaaactta
    14521 gagcttttgg actctgttaa attattcaac tatgtcaatt cttccttatt tacaataata
    14581 gaattaatag atgaaaactt aaagagcaaa tttaagctag gatatatact acattgctct
    14641 tttatgatag aaagatgttt taaaaataca acttttccat ataataacat tactgaatta
    14701 attagtaaaa atcatgaact atatagttta atacgtgaag cctttaagcc aacagaagct
    14761 tattttaata ttttgatacc agacgaagaa attgcttata taatagacat gattaatgag
    14821 tatctaaata aataaaattt atagcactta ttttgtgcat attaattaat atatgaaatg
    14881 gaggacgaga ttttgaaagg aaaaataaaa cagttaacaa tacttgcatt aatttttata
    14941 tttataactc ctgtatttgc atttgcagat actccaccag taccaaattc ttcaagagca
    15001 gcactactta tagaccagga aactaaaaga atattatttg aaaaaaacat tgatgaaaaa
    15061 atgccattag cgagtttaag taaaatgatg acctttttat tggcaataga agcagttgat
    15121 aaaaatcaag ttaaagagac tgatatggtg aaaatagaca agtctacagc ttcagtagga
    15181 ggttctactt gtaaacttaa agatggtgat gaaatatctc taggagagct tatgcagggt
    15241 ctaatgctag tatctggaaa tgatgctgct atagccatag ctaaacatat tggtaaaact
    15301 gaaaagaatt ttgtaaatat gatgaacaaa aaagcagaag aaataggaat gattgatacg
    15361 tattatttta atccaaatgg gcttcctata tatactgacc cagaacataa agaacctcct
    15421 attgaaaata tgtcaactgc tcacgatata gttacacttg gaaagtatat gtacgaccat
    15481 tatgaaaatc aagttacaag aataaccact atgcaagtat ataatgatac taaaaaggat
    15541 tttacacatt ataacacaaa tcctctgctt gtttcagtac ctggtgttga tggaataaag
    15601 acaggatata cagataatgc tggatattgc cttgcatttt ctatgatggt acctaaagat
    15661 gctaaaaacg aaagaaatca tagattgata ggtgttgtac ttggtgatgg taacaagaaa
    15721 aatagaattt cttcttccgc tactttattg aaatatggta aagataattt ccattctaaa
    15781 aagatagcac ataagggtga tattatagag actccttgtg ttgatggtat tgatgatttt
    15841 aagataacag ttaaggttga taaagatttg tatggtgttg tttcagataa cgaaaatatc
    15901 aatcctaagg tagtgtttaa aaatatgaat tatcctatcc ataagggaga tattgttggt
    15961 gttgcaaagt attataatga ctctggtaaa tttgttggaa gtgttgatgt aaaaagtgaa
    16021 agtaatatag gatgtattcc tttgaaggat aagattaaga ttaaggttgc taagataaat
    16081 aaaaaacttg agattaaaaa ttctgtttgt tttaaagcat agtaaagcat attattaaaa
    16141 gggtatgtta aatttataaa gactccactt tgtggagtct ttttgttttt tacaaaaaat
    16201 taaaatagtg gcaacaattg aggatgtgag ggagagttgc cactattttg tatagggtat
    16261 tatattatgt ttttaagcta gttttactat cttctatgtt tatataataa ctcttgtgtg
    16321 tgaatttaat tgataattaa tatgaaaatt atgtgaagtt ttgatttatt tgtaatatat
    16381 tctactacta ttataatttt tctactggat atactaatca tttgtttaac attatctgag
    16441 attgatttgc aaacttttat ataactaaat tagttatata acttatagat tccattaaat
    16501 accctaataa tcatatatgt acatacaaag tcatgcttat gcaaaatctg cagtttcaac
    16561 taacttatta tataaaccat ctttatgtag aagttcagca tcatttccag attctattac
    16621 tctaccttta tctaatacat atatattatc agcactctta attgttgaaa gtctatgagc
    16681 tattataata actatcttat cttgtatgta ctctttcaag ttgttcaata tctctttttg
    16741 tgaaatatta tctagagctg aagttgcttc gtccagaata agtacctttt tattacttac
    16801 taaagctcta gctattgata tacgttgttt ttgacctcct gatattttga cacctttttc
    16861 tcctactact tcgtccaatc ccttatctag attttttata aaatcatcta aacaagtaac
    16921 ttctaatatc tcatctaatt ctttatcact tattttatta tttaatacta tattattttt
    16981 tattgtgtca tcaaatacaa attgatcttg agttactata tcaatattat ttcttagtga
    17041 ttttaaatta atatccttta tattaacacc atctattaat atatcacctt catttatatc
    17101 ccaaagtcgg tatattagat ttgttattgt tgatttgcca catccacttc caccaacaat
    17161 tcctgttact tcccctttct caaattgtaa atttatatca tttaaaacaa ggtttaaatc
    17221 ctcaccatat ctaaaagata catttttaaa atctattttc ttaatttcac tgttatctat
    17281 tctatatcca ttattatcaa ctttaatatc tatcttttca tctattactg aaaatactct
    17341 attgatagac acttttgatt gtgctattct agtattcgcc cttactatag ctatacatgg
    17401 tccaattaac attcctgtat attgttgaaa tgctattaac tcccccatcg tcatcttatt
    17461 atttattatt ttatatcccc caattccata tattaatatt gttatcaatc ctgttagtat
    17521 ttgagatatt gctatattac tactcataat gatgttcaaa ttcaaatctt tttttatcaa
    17581 atctctctct ctctttaaat acttattaaa gaaattcttt tttgaattta atataacaat
    17641 atccataata ttagatacgt actcttgaat caaatttgat aaactgcctg aatcacttct
    17701 tatttcagaa attttcttag atataagttt tgtcatttta ttttgagaaa atgttaatat
    17761 caactgtaaa aataaaacta ctaaaaataa atcaaactgc aaattaatta agaaaattaa
    17821 cgatattata gctgtaaaca catttacaat aatatcaaat ataatctctg ctccaaagtt
    17881 ttctatcata aatatatcac tttccataat ggataaaata ttacctgttt tcttacttga
    17941 ataatatcta ccagaaagtt ttgatatatg gttaagcaac tttactttta gatttatagt
    18001 aactttattt tttattgaag tatataaata tgataatata acatcaaatg tagctgataa
    18061 tatccttata aaaatatata tcaatattaa atttataagc agaaaaatat ttttatttcc
    18121 tattccatca tctattatac atctaattag ttgaggtatt acaatattta cgccagcaat
    18181 tataaaaacc attataaatg atactatcaa cttagtctta ttcttatata ctattatatt
    18241 tagtattcta aataaattat tattaatagt ttttttcatc tatttttcca tcttttatag
    18301 ttataattct atcagctgtc tttgcaatat tatcattatg agttatcata actagtgttt
    18361 gtttgtactc tttaacacac cattcaagca tattcataac ttcatttgta gtcttactat
    18421 ctaagttgcc agtaggttca tctgcaaata ttactgacgg tctattggct aaagctctag
    18481 ctattgcaac tctttgttgt tgacctcctg atagttcatt cggatatttc tttatttgtt
    18541 tttcaatccc caacttatta attaatctca taacatattc tttatcttct tttttcccat
    18601 caagcaacac aggaagtact atattatcat atacatttat aactggtatt aaattgtaac
    18661 tttgaaatat gaagccaaat tcctgtcttc ttatttcaga cagcttatca tcatttaatg
    18721 tatataaatc tacatcatct aagtacacat tccctaaagt tggcttgtct aagcctgcaa
    18781 cacagtgaag tagtgtactt ttaccagaac cacttgcacc tacaatagct gtaaatttat
    18841 gtggctctat atctaaatca ataccatcaa gagctctaac ttcattctca tattttccat
    18901 atattttttt taaatctttt accttaagta ttttcataac gtatctccat tcccatttaa
    18961 aaaacatata tttttaaatt aaattttcgt aaataagatc tactgccata tctaaattct
    19021 ctaattgata taagtatact tctttgtagt caatactaat attaaactct ttatttaaaa
    19081 tagttaaaaa attatatgca ttataatttc caatacatgt aaaattgtta aataaagaat
    19141 attcacttac aaattcaaag tctattttta ttttagaaaa gtttctattt atcacattta
    19201 ttattttcct tgctatatcc aatcttatca aattagattt ataacttgga gtgttcaagt
    19261 tttctatttt taaatcttct ttacttttgc ttttttgaat aagtatttct tccaattcat
    19321 taaacttaat atatggattg ttttttaata tatttgcaac aatgccagtt gccactgcac
    19381 aagccttact atttttacca aaatgagtta ctttatcttt aaatttaata aaattatctt
    19441 ttgaatttgc atacatttga atcttatttt ctggttcgta tatatattga ttatttttat
    19501 atatattttc attacctact acaccaataa cactttcaaa tatagtagga atactttctt
    19561 tatcttcata tttattatgt tttgaacaaa taataaatct accttgacta tttaaaatat
    19621 tacaaacctt ttctaattct ttttcataca tactattaat agatgcagct gaaatgttta
    19681 ttatctgtat atccgacata gtcaacttgc tcagtgtact aagaagattt aaagaacttg
    19741 tttttccaga atcattaaat atattaatag gataaatttg ggtattttta cttatattta
    19801 atatggtttt cgatacaaaa gttccatgtc catttatgtc atttatttcc ttccccccca
    19861 aatctttcaa ttgaaaattt gaatcataaa cataatttcc attaatatca tcatcattta
    19921 catctaatcc tgtgtctaag actgctattc ttactttagt caattaataa tcacctctaa
    19981 aataaattac cttgaatata aaactaatcc ttaccataat ataggttcca agatgttttt
    20041 cattttttta caatttgcat caaacatttt tttgttattt tttaaagtat atattttcga
    20101 tggacaagtt gtacaaaata aatttacttt acattcttta caactctcaa tatttactgg
    20161 ttttaattta tcaaatatat taaatatatc catttctcta ttaagaatcc tctctaatat
    20221 gttttcatta aattctaata tattgaatat ctttaaatcc ttatactcta agtttggaca
    20281 cggatataca tctcctttat gatctatact tatctgatta acacctgctt tacacatatt
    20341 tgaacataaa ctactttcat ccacataatc atctttgcaa atgtaatcaa tatcaagttt
    20401 atcattaaga tatttgctat agttctcatt acctctacca atattcataa aatttcttat
    20461 tataggcgtt gtacctaaat cttcatttaa tttattgaat ttctctatgt ctttctcatt
    20521 attctttcct accaccatag acaaacctac gttctcgaat ttttttagtt ttataagtct
    20581 aatcacattt aatactttac agaatactcc tttacctctt acttcactac aacttaattc
    20641 atcataacca tctaaactaa tttctattgc atataaacca ttaattatat catcaatctg
    20701 tttttcttta attaaagttg catttgtaga cagaattatt ttacctttaa atttttcttt
    20761 tatataatcc aacagctcta agaaatcatt tctcatcaat ggctctccac ctgtaatggt
    20821 taatatcctt ggatttaatt taactatatt atctattact tttcttactt gagatgtatc
    20881 taaataatcg ttattatcta ttttagaatc tacacagcaa taatcacaac ttagattaca
    20941 tctattagtt ggagaaaatg aaattttgtt tattctctct agtctaaatt cacttagaat
    21001 agaatcactt agaagtccta tatgctctaa cttatctata actcgttcat aatatttttt
    21061 atcttcattt tcttggaata catcatacaa attaccaagt tgtaatgcat tatctactga
    21121 atactttaga acttcataac attctattgg aattttaacc catcttccat ttactttatt
    21181 actaagaata acttgattat tatttattat tttttcacat atacaatcta aaaaatattt
    21241 gtttgagcta ttcatttatt ttatttcctt cctatatatt taaattagaa atttcattaa
    21301 ttttctttta taccacttac tatactaagt ttatttatca ttttattaga aaaataaact
    21361 gcaatgacac aaacaaaaac agatatagat ccaaatatta gaatttcata tattggaatt
    21421 tcaaatttaa tagagttatt tataccatat acctgttgat taagtttgtt agtttcagaa
    21481 actattttat aataattgaa actagatata attccagcta caatcatact taatacccca
    21541 tatatcatat tttcttttat taacattctt tttacatttt ttttggacat ccctattgct
    21601 ctaaatgtac atatctcact caacctcaca attatatttg atctaactat aaaaataatg
    21661 tttatagatg atattaataa aactccaaat acacaaatca ttgctaattt atatgaggat
    21721 ttattataat ggtctccaaa atgattacgt acatatcttc cctctatatc tgaaaatcca
    21781 cttttttcta ttatattatt tatttctttt attacttttt tatctgtttc tttttttatc
    21841 ttcacaaaaa ctttattata atcacttatc cctgttaatt cttttaaatc cttttcatta
    21901 aatataattt gaattccacc tgattgagat tctccatctt gctctgatat atagctattt
    21961 ttcattattc cagcgattct tacttttgta tttatatatt tatactctcc atctttatat
    22021 acaggaattt ttatatctat taagtctcct attttaggtg attttataat ctgagtatta
    22081 tttgacaatt taattctaga ataaaaatta tttaccaata aaacattttt ataatccctg
    22141 tctgtataac tataaacatt ttcgccttta tctatatatt tatctatatt atttaatagc
    22201 tctttattgt aacctcgaat taaaactgta gactcattat tattatttat ttcagttctt
    22261 tttagctcat ctaaataatc ttcttctaag tcagcatttc ttagatatcc ttttaaataa
    22321 atattgggtt caacatctgt tacatcttca atagctttta tttcatttaa taaagaatta
    22381 tcatatttag aaaatgtata atttgcattt attggtgaat atcccaaata aatatcaaaa
    22441 ttgccatatg atttacttgt tatattagct gaatctgact cttttccata tatgaatttt
    22501 gtagaagtta gtctgcctat aaataaaacc ccacatagtg atatcgtaat tattattatt
    22561 attgatgtaa ccttattcct aaatatattt ttaaaagcta tctcatttat aattccaaca
    22621 acttttttat gtgattttaa caacaaaagc ctaggaatat aatttaactt atctgtcttt
    22681 tttatagcat caattataga taaatttcca gctttatata ttggtactac agaggctaat
    22741 ataactgcta taattgacat gatgatagat tcaattatag agtttgtatt tatagttaat
    22801 tctgcacttt tatcatatac tatcatgaga cctaagtaag aaaaaactat acctccaagt
    22861 aaaccaatta ctatacctac agataaaact acaatactct gaataaaaaa tacctgtcta
    22921 atatttctct tgctagctcc aacagctcta agtaaaccaa tttgcttggt catatctagt
    22981 attattatgt taaacatatt gtatattact aatgttgcac ataataaagg gagtaagctt
    23041 tgctttattg aataagttgt tttttttgaa atatttttca tatacataga catacttact
    23101 tctgtatttg gataaaattg attacttgtt aacttatatt ttctccctag attcactact
    23161 ttttgagaat ctaaatcttt tgtattcaat tttaatgtac ccatatgagt tattaattca
    23221 tatggaacaa tatctttgcc attgtttgaa tatgtaaaag ctcttacctt atatttattt
    23281 aaattttcat agtattcatc agatttactt aaaataccaa ctattttaaa ttgcttatct
    23341 ttactataaa tagaattttc attattttta tttatatgct cattaacaat ctgaaaatca
    23401 atagtttgat ttaatttgac atctaattcc attttgtcta aaactttttg ttctattact
    23461 aattcattag aagttctagg ctctctacct ttaactaact tatatctagt agaatctaaa
    23521 aaatctttat tgtaagagca taaatttaga gatgctccat tatctttact tataatttct
    23581 ccaaatttaa gtacatcatt gaattctttt atatcttctt caccattaag ttcttttact
    23641 atatcactat ttatatcttc ataaaaagca tgataatctc ccgagttttt gtatgcatga
    23701 tttatatctg attgtccttg agattcatta ataacactta atgaaaaaac tagcataaca
    23761 gcaatcccta tactaagtat taatgataaa gtttttcctt tttgtctctt taaatatgac
    23821 accgcaagtt taatatttat cataatcaga atcctcctta ttcatattta caagaaaaac
    23881 tctataacct aaaatagtag gttatagagc actattcatt aaatactaac taactcgttg
    23941 tgctagttaa atttaattat taaaaatact agagttgaga aaatcacatt tcatttgtaa
    24001 aaaactacat ctagtacttt caacatattt aatagactga ttagtatttt ttaataagct
    24061 actgttcctt ttggagttga tcctgcacaa tccaccatac agtctatttg tgaaattatt
    24121 ccatgcttat cataatacca tttacatgga tctgcttgca cctcaacatc ttgaagttct
    24181 attttttctt cgattttttt tatatgtatc ataatacata tcccccttta cttttgtttt
    24241 atatttatta taactagtta aactagatta taaactacat agtttttttg ttgtttccac
    24301 ataaactctt tctaatccac aataaattgg tactgcactt tggaatttac ctgttacctt
    24361 tttattgata agtttacatc tggttgacat acaataatca agtctagtac atccatcaca
    24421 ctcttcattc ttagatatat aaatttttat aaggttctta atttttttat catctaatcc
    24481 atcatatata tttcctagta aaatatcttt atctcctaca catactgtac atggatatac
    24541 atctcctcta gtatctattg cataactatt tatccctcca aaacaatctc cttttttctt
    24601 atttataaat tgtttatcta aaatagatat atctatatca ggataatcct ttttaatatt
    24661 cataatttta ctaatttgtt catttaatat tttaatatga gaattatccc aatttttatc
    24721 cataaaatct gaaattggtg cgatattttt aaacccttgt tttgctatat catatacact
    24781 ctcataaaga tacttaacag tatttgagtt atatgtcatt cttgccctaa catatatacc
    24841 tttatctata agtctttttc cattttccat tactttttca taagtaccta ctccgttagc
    24901 atctattcta tttatatcat gtatattctt ttttccatca atacttatcg atatgttttt
    24961 tacattcttt gctataaact ctattatttc atcatttata atagttccat ttgtagttat
    25021 atcaaataat atctttttgt cactcaatct atctactaaa tccctatgta tatatttaat
    25081 caaatcaaaa tttataaatg gttctcctcc atgtaaaaca attgttgtat aatcactatt
    25141 actttctaat tctttattac aaataaaatc tattgtttta tcagcagttt cataactcat
    25201 attttctttt tttttatcat taccttcata acaatatgta catctcaaat tgcagtcttc
    25261 agttacaaat atagtaaatt tcaattaact ttttcatctc cccatacagc tgtatttaaa
    25321 ttgtgtttca tcatactaca ccatctatcc aattccaatt cattatcctt aactgaatct
    25381 aatatcgctg ggcaatgcca acaaaaaata tttacatcac aatctttaca tttttcaaag
    25441 ttatatggat aaatactttc taatttaatc aaagacttta gatctcttgt tttattttct
    25501 tttataactt ttacaaattc attgtcaaat atatttccca ttttatagtc atcctttatc
    25561 agtgaaggac aaggatatac atctccatca taatttataa atatttgaga gtttcctgca
    25621 ttacatgcac aacatcccat ttttttattt tttttatctt tacacatttt taattcatta
    25681 tctaaaatat acggaattgt aattggaaga actgtatctt caattaaata gctttcttta
    25741 ttttctaggc ctcttcctgt tggaataaaa tatctttcaa caaattctgt tccaagcttt
    25801 ttatttaaat ctttaaattg tttatttaaa tgagaatttt tttgtccaaa taccatagac
    25861 agctttatat tactgaatcc attgtctttt agcagattaa cagagcttat tactttatta
    25921 aatacacctt ttcctcgtgt ttcactacaa gtattttcat ctactccatc aatactaata
    25981 tctatttttt caaaattagt tataataaaa tcaacattat gttcatctat taaagttcca
    26041 tttgtggata atattaattt ggtatcaggt aatttttgtt ttatatattt aattatttca
    26101 aaaaaatctt ctcttatcaa tggttctcca ccagttataa ttatatgagg agtatttaaa
    26161 actgatatta tatctacgat attttttaaa tcatatgtac taagtaaatc tttcttatta
    26221 gaagaagatt caacacaaca atgtttacaa aataaattgc atctatttgt caaacaaaaa
    26281 taaattgcaa caccattgct taacatatca aagtttaaat tgttactttt aaaaatttga
    26341 tttttgttca taatatctat aagattaata atatattttt tgtcactttc atcatcaaaa
    26401 taatttacaa aatcataaat ttttatttta ttttcttgtc ctaaaagaaa atacttatat
    26461 atttcttcag acaatttaat atattttcta aatttcttat tataaataat tactagttga
    26521 ttttttctat aaataattaa atcttctaca ttaaaaatca tttcttcaga atctatcaca
    26581 actaagtcct cctcaaatta gtaaaatatt tttctttaat atttattact aatatcaact
    26641 ttactatcta attagttatt taattgttgt ttttatgttc ctagttataa agtttatcaa
    26701 atttacattt tattttttta tactatatag aacttttggt caataatgtt accatactaa
    26761 ggtcgcattt gcagataaaa ataactctat ttttctaaat ttatttaaga tatatcatgc
    26821 aaataatata actataaaac ctatcaacat tttaaattca aacaaatata aatcacattt
    26881 tttgttctat tattttattg agtactaaaa aggatgttat cttattagca tagcattcaa
    26941 ccatttttat cacctccaaa tatcctatgc cttaaagata gcatcttcta ttttatcttt
    27001 caatactact gctccaactg ggcaatttag agccccaact agaattagtt cctagttaat
    27061 ggtatataaa tagtaattgt aaacttgttt ttatctgagt atatttccac atttccatta
    27121 tatttttcta ctgaactttt tacactactt attcctattc catgtaaaaa ggaatctttc
    27181 ttacttgtaa taaccttatt attttttaat acaacttcat ttatctttgt attttcacat
    27241 tttattacaa ataacctatt tataacagtt cctcttaatt taatcttttt ttgtatactt
    27301 gtgtccttaa tcttaataca agc
//
LOCUS       DAELTA010000040        26009 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.040, whole genome shotgun sequence.
ACCESSION   DAELTA010000040 DAELTA010000000
VERSION     DAELTA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.040"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            172..555
                     /locus_tag="KPG13_001450"
     CDS             172..555
                     /locus_tag="KPG13_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0985186.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            557..907
                     /locus_tag="KPG13_001451"
     CDS             557..907
                     /locus_tag="KPG13_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985187.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1008..1466)
                     /locus_tag="KPG13_001452"
     CDS             complement(1008..1466)
                     /locus_tag="KPG13_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985188.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1511..2299)
                     /locus_tag="KPG13_001453"
     CDS             complement(1511..2299)
                     /locus_tag="KPG13_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0985189.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2438..2875)
                     /locus_tag="KPG13_001454"
     CDS             complement(2438..2875)
                     /locus_tag="KPG13_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG0985190.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3697..4206
                     /locus_tag="KPG13_001455"
     CDS             3697..4206
                     /locus_tag="KPG13_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985191.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4612..5148
                     /locus_tag="KPG13_001456"
     CDS             4612..5148
                     /locus_tag="KPG13_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0985192.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(5575..6375)
                     /locus_tag="KPG13_001457"
     CDS             complement(5575..6375)
                     /locus_tag="KPG13_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985193.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6570..8168)
                     /locus_tag="KPG13_001458"
     CDS             complement(6570..8168)
                     /locus_tag="KPG13_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0985194.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8496..8672
                     /locus_tag="KPG13_001459"
     CDS             8496..8672
                     /locus_tag="KPG13_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985195.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8846..9976
                     /locus_tag="KPG13_001460"
     CDS             8846..9976
                     /locus_tag="KPG13_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0985196.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            10217..10567
                     /locus_tag="KPG13_001461"
     CDS             10217..10567
                     /locus_tag="KPG13_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0985197.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10575..11024
                     /locus_tag="KPG13_001462"
     CDS             10575..11024
                     /locus_tag="KPG13_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG0985198.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11126..11701
                     /locus_tag="KPG13_001463"
     CDS             11126..11701
                     /locus_tag="KPG13_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0985199.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11694..13103
                     /locus_tag="KPG13_001464"
     CDS             11694..13103
                     /locus_tag="KPG13_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG0985200.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            13287..14720
                     /gene="eutA"
                     /locus_tag="KPG13_001465"
     CDS             13287..14720
                     /gene="eutA"
                     /locus_tag="KPG13_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG0985201.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14738..16102
                     /locus_tag="KPG13_001466"
     CDS             14738..16102
                     /locus_tag="KPG13_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG0985202.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16115..16996
                     /gene="eutC"
                     /locus_tag="KPG13_001467"
     CDS             16115..16996
                     /gene="eutC"
                     /locus_tag="KPG13_001467"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG0985203.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17016..17669
                     /gene="eutL"
                     /locus_tag="KPG13_001468"
     CDS             17016..17669
                     /gene="eutL"
                     /locus_tag="KPG13_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG0985204.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17680..18381
                     /locus_tag="KPG13_001469"
     CDS             17680..18381
                     /locus_tag="KPG13_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0985205.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18384..19853
                     /locus_tag="KPG13_001470"
     CDS             18384..19853
                     /locus_tag="KPG13_001470"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG0985206.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19949..20236
                     /gene="eutM"
                     /locus_tag="KPG13_001471"
     CDS             19949..20236
                     /gene="eutM"
                     /locus_tag="KPG13_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG0985207.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20363..21124
                     /locus_tag="KPG13_001472"
     CDS             20363..21124
                     /locus_tag="KPG13_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBG0985208.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21137..21766
                     /locus_tag="KPG13_001473"
     CDS             21137..21766
                     /locus_tag="KPG13_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG0985209.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21808..22533
                     /locus_tag="KPG13_001474"
     CDS             21808..22533
                     /locus_tag="KPG13_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG0985210.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            22546..22818
                     /locus_tag="KPG13_001475"
     CDS             22546..22818
                     /locus_tag="KPG13_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG0985211.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22811..23362
                     /locus_tag="KPG13_001476"
     CDS             22811..23362
                     /locus_tag="KPG13_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG0985212.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23374..24465
                     /gene="eutH"
                     /locus_tag="KPG13_001477"
     CDS             23374..24465
                     /gene="eutH"
                     /locus_tag="KPG13_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG0985213.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            24467..24940
                     /locus_tag="KPG13_001478"
     CDS             24467..24940
                     /locus_tag="KPG13_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG0985214.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25060..25161)
                     /locus_tag="KPG13_001479"
                     /pseudo
     CDS             complement(25060..25161)
                     /locus_tag="KPG13_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            25456..25977
                     /locus_tag="KPG13_001480"
     CDS             25456..25977
                     /locus_tag="KPG13_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0985215.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT        10240 a         3240 c         4320 g         8209 t
ORIGIN      
        1 ttaataaaag aaaaatatat agaaaatcct tataaaaatt taaattgtaa tgaataggaa
       61 gtaattaaaa agaattttta ttgattttta aaaatttagt tagaaactat atcattatgg
      121 taaaaaatgt attataatgt ttagaaatat tataataagg agcgattaaa catgaatcta
      181 tcgaagaaaa tagaagataa aagaaaagct aaacagagag ctgaaaaagt taagaaagct
      241 aaaattgcta cagctggtgt ggtattagga gctgttactg gtgcagtgag tggagtactt
      301 ttggctccta aatctggaaa agaaacaaga gaggatataa aagatgcttc acaacaaatt
      361 gctgaaaaga taaatatgaa aactgtagat gtaaaaggca aagtatctga gaagctagag
      421 gataaaaaag gaaactttat agaatcaaag aaaaaaataa aaaaatatat agataacaaa
      481 aagagtgttg aaaattctag tgaggaagat aaaataatag atatagttga accagttagt
      541 gttactgagg aataatatgg acaatacaat aacgattaac ataagtataa tatatgtatt
      601 attagctata cttgcttgta ttgctttagt ttatttaatt attgtacttg ttaaactaca
      661 taagatttta aataatgttg gaactatgtt aaatgaaaat actcaaaata taaatacaac
      721 tttaaataat cttcctaatg ttgtagaaac ttttggagaa gtaggagaaa atatgaaaga
      781 tgtaacagaa gtagttactg aagttacagc agattttctt gttactaaag acagtgtcaa
      841 atcaaatatg gatgttatag ttgatatatt gactataatt aaaaacattt ttctagataa
      901 aaaataataa gtttaattaa aataagccta taagatataa tttaaacgac cttaaaatag
      961 catttataag gtcgtttttt attagcttga tttgacacta tgtgttttta ataattggta
     1021 atattcctct ttatcatttg aacatatata tctatgcttg ttgtttcaat tacatccata
     1081 agaacatctt tacattcaag ctcaaaatct tccattcctt ctaaatattc atcatcttca
     1141 tcaaaaaaga tataagaatt atatagccat tcttcaaatt tttcttcaag tatatcccaa
     1201 tacgaactcc aaaataatcc acaacctgat atataagatg ctctactttg atttatataa
     1261 acctcctcta gaagcttttg taatctactg ctataaagaa aatctgctct tccactgctt
     1321 gatattaatt catttaaatc ttctattgac attaattcaa ctttaccact tttatcataa
     1381 ggagtataat tttcatcaac ccatttatgt ggtttgtttt ctctgcatac tgttgctatc
     1441 aactctacaa cttttgttct tatcattttt ttatcctaac ttatttattt tttataaaat
     1501 aatcatgatt ttataattta tctatttctt gtaatgctaa tttaacagtt tcatatctat
     1561 cagcaccatt tattactaca tcacctttta caagtttatt agcttctcca cctacaacat
     1621 aaaccttttt acctaaacct tgcttataac tagacgcttc aattattaaa gaatctttca
     1681 attgccaatg taaaatctga gcagccactt tatcagcatc acctgtataa acgattacat
     1741 tttcatacac tttattttcc cctccctctt gagaatttga tatagatttg tttagtatac
     1801 cttctgctat caatttagca actttatctt tattattaac ataataatct gtatctgctt
     1861 tactatctac aaaacatact tctattaata ttgctggtgc aatagtatga ttaagccaat
     1921 aaagacctct aacatcagac tttgcgcccc tatctttgaa tactgtagct aacctagtat
     1981 tcactctttc tgcataggtt ttgccattat tagtcttata tattgtttct gtccctactg
     2041 gagttgaagt tgtggcattt gaattaaaat gaatctgaac agccaaatca gtttcctgtt
     2101 tatttgctat agcacattgg tctgctaaat gattacttga ttcatcaact cttcctgtat
     2161 aaacagtagc tccacctatt ttaagccatt taacaattaa gtcattcagt attcttgttt
     2221 ctttgctttc gtttatatac ccaacagctc ctgtaccttt tccaattaaa gtgtgccctg
     2281 gtattattac tactttcaaa ctaacatctc ctttacaatt aaatttaact tctatttaat
     2341 atatgcataa cctagaattt tgtttctttt tatatcttat acttttatta taacccattt
     2401 tgtaaaaata tagaatatta agtaaataat gttaaaatta taattttgta tataagacat
     2461 tacttgattg caataatttt atagcatatt catctattct atatccagtt ctataatata
     2521 tatgtttaat tccagcttga attatagcct tagtacaatt taagcaagga aagtgagtta
     2581 cataaataga gcaattttta actgaaatac cttcttttgc acaataataa agggcatttt
     2641 gttcagcgtg aatggttcta atacaatgtc catctctcat ttcatgacca acttcactac
     2701 aatgcttatc tccagaaata ctaccattat atccagtaga cactattctg ttttctgaat
     2761 tgacaattat agctcctaca taagctctat cacaagttcc tctttcagct actgtttcgc
     2821 acagtctcat aaaatattct tgccatgagc atctattttc aagtttttta ttcaaattat
     2881 atacctccaa aacttatatc taatatataa gatttatacc ttaaaaataa tattaaaaac
     2941 aaatttatac gataaataaa atttattaat actgttttta gtctaaatta tttcatttta
     3001 aattaaagtt acaaggaatc taaaattata tatatctttc ttcgtaatat taatgttaca
     3061 atataaaaaa ctgtctcaat aaaatagaga cagttttaca gtcaaagttt ataatacaag
     3121 ttcatatttt tctttaaatt cttttacagc ttcgggccat gccttttgtt ctgtaatacc
     3181 tttttcttta gctattttaa ttgcaatctc tcttatcatt gcagcgcctt ctaatattcc
     3241 catttttaat ctcccttcaa atatcttttt gtatagaata taaatagtgc taagatttat
     3301 tacatgctgc atatacaata tctatacaac acataacttg taaattcctt ttattttcat
     3361 acgtctttaa ccgacatata attatattca ataagtttta aaattatgaa ctcaattata
     3421 attattttta tttttttaga atctaaacac taaaaattgt aggtaaattt gtcttatgcc
     3481 tttataaata tttctacagt tttaattact ggacatatct atttttatta taaaaagcga
     3541 ttaaaaatcg agaatatccg cataaaaact gtatataagc aaaacggtta aaggaaaaag
     3601 aagaaattta cttgaatgtt aaaatgcgtt gcttgaagca atgggcagtt ttgcctataa
     3661 tatcggtaac aactgaaaga aaggtggtta gccctaatgt taagcgaaaa cattaaaaca
     3721 atcaggaagt caaaaggact ttcacaacaa gaacttgcta taaaactgaa tgtagtgcga
     3781 caaacaatat ctaaatggga acaaggactg tcagttcccg attctgatat gttgatttcc
     3841 atatcagagg tgcttgaaac gcccgtaagt actttacttg gagaaactat tgttgagtcg
     3901 gaagttgata acttaaaagt gatttctgaa aaattagaga ttataaactt gcaacttgca
     3961 caaagaaaaa acatgagaag aaaaatgttt cattggttat ttattttatt atgtgcagtc
     4021 atattaatag tttttgtggt cttaggggga ctaaatagtc cttacttgga ttggaattat
     4081 accgaccctg aaaccgctgt ttttggagta gcttttcatg cttttgagtg gctatttgtc
     4141 agattagtac cgattgtcct tataggagca attattggaa tctttttgac acgtaagaaa
     4201 atatgaagta actctgcttt ttataataca gcaagcgttc cttaaaagcg tcattctaca
     4261 tggcaatcga ggacgactat gtaaggaaga acctttttga tttccaacta agcgaagtta
     4321 ttgaggacga cagtaagacc aaaaccgcat taagtaaagt acaggaagca agactgctgg
     4381 acttatactc tcatgcgtca gatgcaggaa atgcgaggtc tggtagcatg atttacaacg
     4441 atttgtacaa cgactgctag tagaaataat atggagataa gtattatcta caaagtcaga
     4501 aaagaaatga cttgataatt tactcatgtt ataattattg atgtgaaatt aataaatcga
     4561 aattcctttt ttggatttaa taatactgag taaggaaaaa gtgaattact tttgcaagga
     4621 ggtgttaata tcaggcaagt aaagtctttt gtttttccag ttatatcctt aatattctta
     4681 gaccaaatta gcaaagttct tataggatta ttcttaatgg actttgaaat tgatataatt
     4741 gggaaatttt taagattcaa tcctgttcaa aatacaaatc tctcttatgg gggaaacttt
     4801 attggtattc tatctaattt atgggtattg gtattgttta acattttagt tatattagtt
     4861 attatatctg gatatgcttt ttataaatca aaaaatgaac aaacaagcta ttcagtaaaa
     4921 gtaattatgt cttgtggact tgctggtaca atatgtagct tgatagataa attattttgg
     4981 ggaggaagtt tagatttttt gcagatacca agctttttta ttttcgactt aaaagactgc
     5041 taccttactg ttgcagaaat aatatttgtt gtcataggaa ttttgcataa tagagaaata
     5101 tcaatgaaag aatacatata tttttgctat cgtcagttta aaaaataaat ttagaagata
     5161 aatttaagtt tcaaggtaaa cttatttaat cacagcaaat agttggctta cttaagcttt
     5221 tgggctaaac tgtgagcact aattaagtct ctgatatcta acaaacggag cacaaaaata
     5281 aaaattaaat ataaagcaca gttaattttg attaaaaagt tgactgtctt ttcttttgta
     5341 gaaatttaag cataggtaat aaagaaatga ctgcatttaa gcattatgta tataattgga
     5401 ggcaataaaa agccagcaaa gcccataaaa tagagctgtg ctagcaacat gatgaattat
     5461 aagaagataa tcaaaaactt acgctttatt ttaatttaaa aaaatgatta ctgtaaagaa
     5521 atcatttttt attataatca ctgatacatc aaatattcta aataaaattt ttatttatct
     5581 ggaattgtat attttttcta taatattaac tacagcgtac attgcccaag cacagatagc
     5641 caatacaaat actcccatca ttactaaatc taatttaaat acttgagaac catatactat
     5701 caagtagcct attccatatc ttgaaactaa aaattctcca actattacac ctacccatgc
     5761 cataccaata ttaattttag ttaaatttat taaatttcct gtgtttgcag gtaatatgag
     5821 tttaaataat atttgagatt tagtagctcc aaaacttttt aacatcttaa ttttttcttc
     5881 atctatattc ataaaataat tataagctga aagtatagta acaacaacag aaatcgttac
     5941 agctgttacg acgattcctt ctattccagc acctacccaa actataatta taggagctaa
     6001 agcagtctta ggaagagcat ttaaaactac caaaaatggg tctaatattt ttgataattt
     6061 ttctgaccac caaagagcaa ttgctaccaa aatacctaat acagtaccta taacaagacc
     6121 aagaacagtt tcatatacag atattccaat atgtttaaat aattgaccac taaatgcata
     6181 ttgaataaat aaattgtaaa tatcacttgg tttgctaaat agaaatgtat ttataacatt
     6241 taagtcagct aaaagttccc agattacaat aaaaccaatt aaaattaata gctggtagaa
     6301 taatacattt cttttttctc tttttacacc tttaacatag tttatatatc cttcagatct
     6361 tttattaagc ctcatgtgta tctagctcct tccataatat gtcgaaatac ttttgaaaat
     6421 caggaacttt acgtgttttg agaggcgttt ttgttccatc catgttaagt tcaatatcgt
     6481 aaatattttt tatggtagct ggtcttttag atagtattgc aaccttatct gatatgctta
     6541 ttgcttctgc tatagctaaa aaagtgcaac taattgctta aagaaacaga attatcaact
     6601 tcagcaccca tataaacaat attaagattt cctaaactgc tatcccaaac gatactatta
     6661 ataatagtag ataataacaa tctcttatca tctaaactgg cattatcaac ttcatcatta
     6721 aatctcttca aattactaac aataatatca atattaatac tgtcactact aatattgatg
     6781 ttactagatt ccaaagtatt aatcttatcc tttaaaatac taatctcatt atttaggctc
     6841 tcaatctggg aaactatata atcagaagca gtactaccag tattttttgc aagctctata
     6901 actaaatttt taataagtct ttccttatct aaaatttgat ttttaagtaa attagtctca
     6961 gatttaaaaa tattaaaaac attatcagtc ttaatgttat tactattgaa tgcacttata
     7021 atagactctt ctttacaatt cttaatttta tctataacta aactttcaac taatggacca
     7081 ttaagatttt tacagttgca actatcagac ccaagtgttt ttttagtacc acaaatataa
     7141 taatacttta aatccttgtc agtttttttg cctttatagg taattctcat attagaacca
     7201 catttaccac attttataag accagataaa agagcagttt ttccagttga tatccttgga
     7261 gttttatcac gatttttatc taattgattt tgtacttcaa gccatatttc aggttctatg
     7321 actccatggt gttttgcaat tgctgctatt gagttagatg tatttttagc ataggtaagg
     7381 attccacatt ttctatctgg agtacctaca acatccatac cactattttt aaggtaatct
     7441 aaaatttcat cattagcttt tacatatgct ggatttctaa gtattctaga taaagaacta
     7501 gggtcaaatt tgccaccacg aggacccctt attccattat tatagacata tttatataac
     7561 ttagataagg acctaagttc taaatattta ttgtatatta gctttacaat ctccatttgt
     7621 tcatcattta cttgaagctt atacatactt ctttctttta gattttcatc taagaatgtg
     7681 attttttcac tgttgaatcc atgaggtgta ttgcctccaa gccatctacc actacgggct
     7741 aattcataca tattgtcttt tatacgttct gctattgttt ctctttctaa ttgagcaaat
     7801 acacttgata taaacatcat agctcttccc attggagttg aagtatcaaa ttgttctttg
     7861 atagatataa atgatatgct tagttcattt aatttttcta ttaaagtaga gaagtcagat
     7921 acatttctcg atatcctatc taatctataa caaactaggt aactaaattt cttatctttg
     7981 gcatcctgca tcattttctt aaattgtggt ctatctatat tgctaccact aaatccctca
     8041 tcttcatata tcaaaaaatc ttttacacca atagactttg cataatcaat acataattgt
     8101 atttgatttt ctatagaatc accttttcca gtaaatcttg attttcttga ataaattgca
     8161 gctttcaaaa ttaaaacccc ctataagtta ggatatatat ttgaaactat ttatctacaa
     8221 ccttttaagt aaacttatta gaatgaattt attaattagg taaatttata taattctact
     8281 ttttataata tatgactatt tattttattt ctttactatt caagttctaa atcaataata
     8341 aattttgata tttaattgta tcaggacaaa tctctaattt catagattat taatataaaa
     8401 tcataggtta gtgtgaaaaa gttgaaattt aaatatttta aaagataatt tttttgaact
     8461 aataggtata acaaaaattt atagggggat tgcatatgga aatcaatgtt atagaaattt
     8521 tccctaaaga taaagctaaa cttaataaaa tagaaatgga taaagctagt tggtttgtaa
     8581 atataataag taaaaaatat cctaaagaag ctttaaatga agcatttagt actttagaaa
     8641 aagaattaaa tataagtaaa gctaatacat aagctttgat tattgatttt atatactatt
     8701 ggttttatac gctatgactt gacattatat taatcaaata gtatattttt aaatatagca
     8761 caatgaagtg ttaaaagagc aatggagctc catagataaa gtctatggag cttttttgcg
     8821 tatttaactt aaggaggata ggactttgaa taactttgag attaaaacta gaataaaatt
     8881 tggagaaggc tcactaaaag ctttgcaaga attaaaaaac aaaaatgtac taataataac
     8941 agaccctttt atggtgaaat caaaaacaat agataaaata acatctaatt taataaactc
     9001 aacatatcaa gtttttgaag aaatagtacc agacccacca atagaagtag tagtagcagg
     9061 aatagaagca tttaaatcta taaatccaga cgtaataata gcattaggtg gtggttctgc
     9121 aatagatgca gcaaaatcta taatggactt ttctaaaaaa atattaaaaa ttaacgaagt
     9181 agagtttata gcaataccaa caacaagtgg aacaggttca gaagtaacat catttgctgt
     9241 aataacagat agtcaaaaaa aatgtaagta tccattagtg agtgatgatt tacttccaga
     9301 tatggcaata ctagacccag aacttgtaaa aactgtacca aattttataa cagcagatac
     9361 agggatggat gtattgacac atgctataga agcttacgta tctacaaatt ctaatgactt
     9421 ttcagatgca ctagctgaaa aagctataaa gttagtattt aaatacttaa taaaagcata
     9481 taaagatgga aatgacatag aagctagaga aaaaatgcac aatgcatctt gtttagcagg
     9541 tatggcattt aaccaagcat cacttggcat aaatcattct attgcacatg tattaggtgg
     9601 aaaattccat ataccacatg gaagaacaaa ctctatatta cttcctcatg taatagaata
     9661 taattcatca atagtaggat atactaacac aacttactct gctacagcta aaaaatatgc
     9721 acaaatagca gaattagtag gacttgggag tgctaatata agaatatctg ttagaaattt
     9781 agtaaatgaa ataaagaaac tacaaaaaga aatgaatatg ccaactaaat tgacacaatg
     9841 caaattaagt ttagaagata taactaatga agaaagagaa atagcaaaac ttgctataaa
     9901 agatacttgt acattaacta atcctaaggt accaactgaa gatgatataa taaacatatt
     9961 aaagaacatt aaataactac attttattac atgtaaatat aatattatac aaatagggaa
    10021 aatgttttta aaaatataaa ttatatattg acaaatataa aacataaaac tataatgaaa
    10081 gtatcacaaa ggcgtgatac tttagtagaa agcaatggag cttcttagga atattaatat
    10141 tattcttatg gagctccttt tattttaatc aaatagcttt ttaataaact atattcaaag
    10201 aaagcaggga ataaatttga ctgaagaatc taagcaaaga gttattcaag aatatgttcc
    10261 tggaaaacag gttacattag cacatatcat agctaaccct aatgaagata tatataagaa
    10321 gttaggatta gtacttgata aaaaagatgc aataggaata cttacaataa caccaagtga
    10381 agcgtcaata atagcagcag atgttgctac aaaggcttca aatgtatctt taggttttat
    10441 agatagattt agtggttcag tagtaatatc aggtgatgta agttctgtag aatcagcgtt
    10501 aaatgatgtt ttagaagtct taggaaatat gttaaacttc tcatctacaa aaatcacaag
    10561 gacataataa tacaatgaag aatataatat ttatgggaaa gacaggtagt ggtaaaacta
    10621 ccttatgtca aaaactagac cagttagaat taaagtataa aaaaactcaa tcagtggaac
    10681 tttataataa tgcaatagac actcctggtg agtatatgga aaatagaaac ctatataatg
    10741 ccttaataac aacatcagca gatgctaaaa ttatagcaat agtgtatgat gccacacaag
    10801 aagaaaatta tatagcacca ggttttgcca gtatgttttg caaagacgta ataggaatta
    10861 taactaaaat aaataaagct actgaaaacg aagtcaatat agcttatgaa agactatcac
    10921 tagcaggagc aagtcaaatc tttaaaatag acacagttga taatattgga cttgaaaaat
    10981 tatttaaata tctagaaaaa tataaaaata tagatgaagt ttaaggggaa atattttttt
    11041 attcaaaaat attattaaag tatgataaaa taaaaaaatg tataaactat tttaagaaga
    11101 tttaatcttt aagcgaggta ggaacatggg gaaaaaaata ctattggttg atgatgaacc
    11161 actgttaagg atggatacaa aagatatatt agaaagtcat ggctatgaaa taataggcga
    11221 agcaagtgat ggatttgaag ctgttgaaat gtgtaaaaaa cacaaacctg atttagttat
    11281 aatggatata gagatgccaa tacttgatgg tataaaggca ggcaaaataa tatgtaaaga
    11341 aaatatagct ggtggagtac ttttattaac ttcatttgaa gataaagaat acgtagaaaa
    11401 ggctaaaagt ataggtgctt atggatattt agtaaaaaca gcaagtgaaa aatcacttat
    11461 cccaaccata gaaatgtgtt taaataaggt aaatgaattt gaaaaaatga aaaaagattt
    11521 agaaaaagta aattctaaat tgaatgaaag aaaattagta gaaagagcaa aaggtatact
    11581 tataagagag tttaagattt ctgaggatga agcatacact agaataagaa aattaagtat
    11641 ggataaaaga actacaatga tggaaattgc caaaatgatt ataataggat acgatgacta
    11701 atacgataaa aaaaatatca actgcacaaa catacttaaa taataacgaa attgaaaaaa
    11761 ttatagaagt atcaaaatcc ttagatttaa tggcttcttt ttatgaagct gatgttttta
    11821 tagatgtact aagtaaattt gatgatgaag caattgttgt ggcacatggt agacctaaag
    11881 aaaagtcaat gtactgtgaa aatgttatag gtaaaaaggc tttaattgaa aatgaaccag
    11941 gagttataaa gtgcttaaaa acaggagaaa gagtaagaga tattaaagct ttaacacagg
    12001 aatataaact ggtcaaacaa aatattcagc caataacact agatgacaaa gttttagcag
    12061 tactaatagt tgaaaaagat ataagtagtg aagttaagaa tgagtttaat gctagtgaag
    12121 acgaagaaaa taaaaattta catcagttta taaaacttat tggaaataat gactttttag
    12181 ttgataattt aaattcagcc atattaatat ttgataaaaa tggaaaatta aaaattaaaa
    12241 atcgtattgc aactgagata tacaaaaatc ttgggtttgg atatgatata caagatatgc
    12301 attacaatga tttagatata gaaaccaaca cctttgaaaa tatattaatg gatgaaaatt
    12361 atgatgagac aaatgaagtt tcaataggta atatgttttt taaaataaag accatataca
    12421 tcaaagatga tgaatttaag gtttgcaaaa tagtacaaga tataagtgat ttaaagagaa
    12481 aagaagaaga attagttttg aaaacagtgg ctataagaga agctcatcat agagtaaaaa
    12541 ataatcttca tacagtaata tctataataa aaaaacaaag tagattatct caaaatgatg
    12601 aagtaaagca gtgtttagat aacataataa atagggtatt tgcaatactt tcgactcatt
    12661 atttactatc taaagaagtt gataataata tatctatacg agaaggtatc aatttattag
    12721 tgtcaaatat tcaaggtggc tatttattta gtaaggatat agatatacgt ataactggtg
    12781 aagattttaa aattagtgga gaaaaagcaa cagcaatact tttggttata aatgaaatac
    12841 tacaaaattg ttatgaccat gcattttcgg atagagaaca tggtactata caaatattaa
    12901 taaataaaga taacgactat agaaatattg ctattataga tgatggaatt ggatttgata
    12961 gtaataatat aaataaaaat agcttaggga catatataat agatagttat ataacacaga
    13021 tgttaaaagg aaatatagag agaaaaagta gtgagaatgg tacagaagta ttaattacta
    13081 tacctacaca aattaatatt taaataataa ctcatctaca aagtagtagt tttaattgag
    13141 caaagaggct taaggttatt aatagattaa gttaattgaa tgtatttata aaaattataa
    13201 gtatatattc aatttaacag gtatttatta taactttaag cctctttgct tgtttaagac
    13261 atacttaaat tttaggaggt gagaaaatga gagaagagtt attaagtgtt ggtattgaca
    13321 ttggaacaag tactactcag ttaatttttt ctaaattaat agtagaaaat actgaatcaa
    13381 atttttcggt acctagaata agtatagtag acaaagaaat tatttataaa agcaaagttt
    13441 atttcacccc tcttatttca aataatgaaa taaatggtca acaaataaaa gatatagttg
    13501 aaaatgaata taaaaaagca aatatatcta aagaagatat acatacagga gcagtaataa
    13561 taactggtga aacagctaga aaagaaaatg caaatgatgt acttcatact ttgagtggat
    13621 ttgctgggga cttcgttgtt gcaacagcag gacctgattt agaaagtata atatcaggaa
    13681 aaggagctgg agctcatatt tattcaaaag aacattcaac tacaattgta aatatagaca
    13741 taggtggggg aactagtaac atagcctcat ttaaaagggg agaagtaacc gatactgggt
    13801 gtttggatat aggtggaagg cttgtaaaga tagataaaat aagtaaaaaa ataacttata
    13861 tatcacctaa aatagataaa atcataaaag ataataattt aaatcttcaa gtaggcacaa
    13921 tggctactgt agaaaataca aagccaatag tggatataat ggttgactta ttaatggaaa
    13981 gcataggatt aaagaaacag acacctttgt ttgattttat aataaccaat aaatcaataa
    14041 gtatagatga aaatattaaa aatgtttcat tctctggtgg agtagctgat tatatttact
    14101 atgatggaga aataaaagat tattttgaat atggtgactt gggaatttta cttgggcaag
    14161 caataaaaaa ttgtgaatac cttaaaaaag taaatgtagt taaatctatg gaaacaataa
    14221 gagccacagt ggttggagca ggttctcata ctactgaagt aagtggaagt acaataactt
    14281 acactagcga aaagtttcca cttaagaact tgccagtttt aaaattatca attgatgaag
    14341 aaacaggcac ttcaaaacaa atcagtgaag ctataaaaaa taaacttaat tggtttaaac
    14401 tagaaaatga tttacagaaa gttgctatag ccatgatagg aaagaaaaat ccaacctttt
    14461 tagatataca agagtatgct aaagggtttg tagatggaat gcaacaaatc atagaaaatg
    14521 atttaccttt tatagtaatt gttgaaaatg atatggcaaa agtgttaggt caagcaatat
    14581 atgcattatt aaattatgaa aaagaaattg tttgtataga cagtgtcaag gttgaaaatg
    14641 gagactatat cgacattgga aaacctatag ttgaaggtaa agttttacct gtggtaataa
    14701 aaacattagt ttttaattaa tagaaggagg taagtaaatg attttaaata caaaattatt
    14761 tggacgtact tatcagttta aaggcttata tgatgtaatg gctaaagcta atgaagaaaa
    14821 atcaggagat aaattagcag gtcttgcagc agaatcagct gaagaaagaa tagcagcaaa
    14881 agttgtttta tcacacataa aattggagga tataagaaat aatcctgcag tggcatatga
    14941 agaagatgaa gtaacaagaa taatacaaga tgctgtaaat gaaaaagttt atgaagaaat
    15001 aaaaaattgg acagtctctg aacttagaga atggctattg gattcaaata ctacaagtct
    15061 tgatataaga agaatatcaa gaggattaac ttctgaaatg atagcagcag ttgcaaaatt
    15121 aatgtcaaat atggacttag tttatgcagc taaaaagata aaggttactg ctcactgtaa
    15181 tacaactata ggagaacaag gaacattatc agcaagactt caacctaatc atccaacaga
    15241 cgacccagat ggaatacttg catcacttct tgaaggttta acttttggta taggagatgc
    15301 agtacttgga ttaaatccag ttgatgactc tgttgagagt gttactaaag tattaaaaag
    15361 atttgaagaa gtaaaacaaa aatatcaaat accaactcaa acatgtgtat tggcacacgt
    15421 tactacacaa atggaagcaa taagaaatgg tgctcctgca gatttaatat tccaatctat
    15481 agcaggttct gaaaaaggaa atgaagcatt tggatttaat ggcgacacta tagaagaagc
    15541 tagacagtta gccttaaaac acggtacagc tacaggacct aacgttatgt attttgaaac
    15601 tggacaaggt tcagaattat cttcagaagc acatcatgga atagaccaag ttactatgga
    15661 agctagatgc tatggatttg ctaaaaagta tgaaccattc ttagtaaata cagttgttgg
    15721 gtttatagga cctgagtact tatatgatgc tagagaagtt gtaagagcag gacttgaaga
    15781 ccactttatg ggtaaactac atggcttacc aatgggtgtt gatgtttgtt acactaacca
    15841 tatgaaggct gaccaaaatg atatggaaaa cttagccata ctattaacaa cagcaggatg
    15901 tacgtatttt atgggaatac ctcatggaga tgatgttatg cttaactatc aaacaacagg
    15961 ataccatgaa acagcatcac ttagagaaat gtttggacta acagcaataa aagaatttga
    16021 tgcttggatg gaaaagatgg gattctctaa agatggtaag ttaacagagt tggcgggaga
    16081 cgcatctgta ttattaggat aggaggtatt taatatgaat gagaaggatt taaaagcatt
    16141 ggtagaacaa ttagttggac aaatggttgg agaattagat actaatgttg tgtctgagac
    16201 tgttaaaaag gcaactgaag ttgtagtaga caacaatgct tgtatagatg atataacaga
    16261 agttgatata agaaagcaat tactagtaaa aaatcctaag gatgcagaag cttatttaga
    16321 tatgaaagca aaaacacctg caagattagg tataggaaga gctggtacta gatataaaac
    16381 agaaacagtt ttaagattta gagcagacca tgctgctgca caagatgctg tattctctta
    16441 tgtagatgaa gaatttataa aagaaaataa tatgtttgca gtggaaactt tatgtaaaga
    16501 taaagatgaa tacttaacaa gaccagattt gggaagaaaa ttttctccag aaactataaa
    16561 taatataaaa tctaaatttg gtacaaatca aaaggtttta atattagttg gagatgggct
    16621 tagctcagct gcaatagagg caaatttaaa agactgtgtg ccagctataa aacaggggct
    16681 aaaaatgtat ggtatagatt caagtgaaat tttatttgtt aaacattgta gagttggtgc
    16741 tatggaccac ttaggtgaag aattaggttg tgaagttata tgtatgttgg ttggagaaag
    16801 accaggatta gttactgctg aatcaatgtc agcatatata gcttataagc catatatagg
    16861 aatggcagaa gcaaaaagaa cagttatatc aaatattcac aaaggtggaa caactgcagt
    16921 tgaagcaggt gctcatatag ctgaacttat aaaaactatg ttagataaaa aagcttcagg
    16981 aatagacctt aaataaagtc gagggagtgt agaatatgaa aaatgatgta attcgtccta
    17041 atatactagg aattaaaata atttcaaata taagtccaga aatggcacaa aaactagaat
    17101 taaaatcaca tcacaaaagc ttaggtttaa taactgcaga ctgtgatgat gttacgtata
    17161 cagctcttga cgaagcaact aaagcttcag aagttgatgt tgtatatgca aaatcaatgt
    17221 atgcaggtgc tgcaaatgct tcaacaaaac ttgctggtga agtattagga ataatagcag
    17281 gtccaagccc agcagaagta agaagtggtc taaatgcagt agttgatttt ctagagtatg
    17341 gagcaacttt tataagtgca aatgatgatg attcaatagc atactatgct cactgcgtat
    17401 caagaacagg tacatacctt tcagaggtag caggaataag agaaggtgaa gcattagctt
    17461 atttagtagc accaccactt gaagcaatgt atgcactaga tgcagcaatg aaagctgcag
    17521 atgttaagat gtgtgaatta tttgcacctc caactgaaac aaactttggt ggagcattac
    17581 ttacaggttc tcaatcggct tgtaaagcag catgtgatgc atttgcagaa gcagtaaaat
    17641 cagttgcaga caatccaaca ggattttaag gatagttaga tggatgctat aggcttaata
    17701 gaagtaatag gatatgttgc tgcaatagaa gcttctgatg cttgtttaaa atctgcaaat
    17761 gttaatattg taagagttga taaggttgga gcaggtattg ttactttaac aatatgtgga
    17821 gatgtcggtg ctgtaaaagc tgctttagaa gcaggtgaaa tagcagcttc cagagttggt
    17881 acattaagaa catcacatat tataccgaga atccataatg aagtaacaaa tgctttattc
    17941 aaaactaaag aacctaaagt ctgtgaagtt tcagaagata aagaaaaaaa ctttgagatt
    18001 acattggaaa tagctgataa tgtatcgaaa aatttatcag aaaaagaaaa tgtaatttta
    18061 gatgtcaatg aaaacagtgg agatactgaa aatttatata gtgatataga taaaacaaac
    18121 aaaaaaattg aaacagttga tgatagttta acagaagcta atgaggaaaa aatagaaaac
    18181 actaatgata aagaagtatt ggaacaagtt aaaatagaaa attccgattt tgatagagat
    18241 gacctatcaa agaacagtgt aaaaaaactt aaagctatgg ctaagaaatt agattcttca
    18301 ataacttata aagacttaaa tgttcttaaa aaagaagaat taataaactt agttaaaaag
    18361 cttagtagaa gggatagata gttatgacac ttttagataa agatttagta tctatacaag
    18421 aagtaagaac tcttgtaaga agatctaaag tggctcaaga gaaactttct aaaatgagtc
    18481 aagagcaaat agataaaata gtaaaagctg ttgtagatgc agcatttaat aatgctacta
    18541 aattagctaa aatggcaaat gaagaaactg gatttggaaa atgggaagat aaagtaatta
    18601 aaaatacttt tgcatctatg ggagtttatg aaactttaaa gaacacaaaa actattggaa
    18661 taattggtga agataaagaa gccaatacga tagatatagc tacaccagtt ggagttgttg
    18721 caggtcttat accttcaaca aacccaacat caacagtaat atacaaaact ataatatctc
    18781 taaaagcagg aaatactata gttttctctc cacatccaaa tgcaaaaaac tgcatacttg
    18841 aaacagttaa tataataagt gatgctgcta taaaggctgg agctccagag gattcaatat
    18901 tatgtataac taatccaagt atagttggaa ccaatgaatt gatgaaacat aaagatacaa
    18961 acttaatact agcaactgga ggagaagcaa tggttagagc tgcttactct tcaggaacac
    19021 cagctattgg agttggacca ggaaatggac cagcatttat agataaatca gcaaatgtta
    19081 aattagcaat caaaagaata atagattcta aaacttttga taatggtgtt atatgtgcat
    19141 cagaacaatc tatagtaata gaaaaatcta tggaagaaac tgttttaaaa gagcttaaaa
    19201 atcaaggtgc atatgtttta aatgaggagg aaagtgaaag attagcaaaa ttcatattaa
    19261 gagctaatgg tactatgaat ccacaaatag ttggtaaaag tgttgaggta atagcaaatt
    19321 tagcagactt aacaaatgta cctaaaaata caaaagttct agttgcaaga gaaaataaag
    19381 ttggtcacaa tgtagcctat tcaagagaaa aacttacacc aatactagcg ttctatgttg
    19441 aagaaaatgt agaagctatt atgaacagat gcagagaaat attgttaaat gaaggtgcag
    19501 gtcatacatt ctcaatgcat gtaaatgatg aagaattggt aaaaagattt gcccttaata
    19561 tgccagtatc aagaatactt gtgaatactc cttcagcact tggaggaata ggtgcatcaa
    19621 caaaccttgt accagcacta acacttggtt gtggtgcaat aggtggaagt tcaacttcaa
    19681 acaatatagg accacttgat ttaataaatg ttaaaagagt agcttatgga atcaaagaaa
    19741 tagaagaact tggaagagat aaatctcaag ttgaaaaatc aatgtttgac tcaatggata
    19801 aagaagaatt aataaatctt cttgttcaaa aactagtcaa agaactagca taactaaaat
    19861 aaacaatgaa acttatggct atcgctataa atttctaaaa atataaacta taaaaaacta
    19921 taaaattaaa tttttggagg ataagattat ggcaagtgca aacgcattag gaatgataga
    19981 aactaaagga ttagtaggag caatagaagc agcagatgca atggttaaag ctgcaaatgt
    20041 acaattagta ggaaaagagc aagtaggtgg aggattagtt actgtaatgg taagaggaga
    20101 tgttggagct gttaaagctg ctacagatgc aggagctgcc gctgccgaga gagttggaga
    20161 actaatatca gttcatgtta taccaagacc acactttgaa gtagatgcta tattaccaaa
    20221 ggtatcagct gagtaatata agaataatta taaaatttaa attttgattg aaaaagaggc
    20281 tatctcaaaa atgagtctta ctaataaact caatgtattt tgaggtagtc ttttttatac
    20341 atctaatagg agatgtgata aaatgggtgt tttaacagaa aacgaagtaa aaagaaaaat
    20401 tggaaaaaac aaaaataata taactgaatt ttatatagaa aaaggtcaaa taataactcc
    20461 ttctgcaaaa tcttatttag ctgagagaag tattgagctt aaatatgttg ataaactttc
    20521 agatgtaaac aatgaaccta aagaagttgt aaaagaagta gttaaagaga aaaaatatag
    20581 atatactact gtatttggcg ctaaattgga tgaaaaacca gaacatatga ctcaattaaa
    20641 tggaaatctt ttagttttta aagaccataa aagaataata ttgagaggaa aaatagattc
    20701 tcttgaatca gaaatactaa aggtacagat actttgtcag aaactagata tgccaaagct
    20761 tgttaaggaa ttgcaagaag tattagactt tgtaagaaat ataatgagat gtgaagtttt
    20821 agatgaaaag ctaggggact ttaaactttt aggattaact cctcaagaac ttagagaaca
    20881 atctcactat cctaaaaaat actttggtat aggacatgag ttcccaagtt atgaaatggg
    20941 agaagtagta gtagctataa atagtataag aagtaataca cgagaggttg aacttgcagc
    21001 ctatgaagca tttaagggtg agtatggaaa tgttcacaga gaagatttaa taaaggcact
    21061 taatagatta tcgtcagtat tttggataat gatatataag gttagaacta ataagtataa
    21121 gtagttggag gacataatgg agaatctatt aaatgaaata gtggaagaag taataactag
    21181 agttaaaaaa gaagcattta tagaagttga agcatcagga agacacgttc acttaagtag
    21241 agaagatgtc gataaattat ttggagaagg atatactctt acaaaactaa aagacctatc
    21301 tcaaccaggt caatatgcat gtaaggaaag agttacaata actggtccta aaggaagtat
    21361 aaaaaatgta gttgtacttg gaccatgtag aaatgaaact caggtagaaa tatctttaac
    21421 agatggttca actcttggac taaaagctcc tataaaacaa agtggagact tagaaggtac
    21481 tttaagtatt aaaatatcaa cacaatatgg tgaagttgaa cttgataagg ggttaatggt
    21541 tgctaaaaga catatacaca tgactcctaa agatgctgaa aaattcaatg tatgtgataa
    21601 agagatagtt gaagctaagg tgatgggtca aagaccttta acatttgatg atgtagtaat
    21661 aagagtgagt gacagtttta aaacttatat gcatatagac tatgatgaag ccaatgcatg
    21721 tggttattca aaaggaactg ttgctaaaat aataaagaaa gcgtaaattt aacctactat
    21781 ataaattacc tctagggagg atttaatatg aattatgata accttgtaga tttaataacg
    21841 gaagaaatct ataaaaaaat aaatagtaat gaaataaaaa tatctaataa acctaaagct
    21901 gtgattgtat ttgaacaaga taacaataaa tttaacttat taaaagatga atttgaaata
    21961 gtagaatttg ataaaagtat aagggaatgt gaaatagtga tagtttcaag actttgtatg
    22021 agggggctat cgaatatagc acttggaaat tcaacaagtg atgaagaaag atttatacta
    22081 aaaatgatta tgaagggcaa agaagtatat gtcctagatg aaggaattga atacaaaaaa
    22141 tataaggata ctgcaccaaa agcattatat aagaaattta tgtcatttga agatgaaata
    22201 tgtaaatttg gtgtagaaat tataaaagac ttaaatgtta taactaaaaa taagttaaat
    22261 ataaagaaag aactagaaag tactagaggt tctaaaaaag aagatacaaa agttttagat
    22321 aaagatgttt taaatttaac cagtgtagaa agtgtaaaaa tagataatga atttagttta
    22381 gatttaagga ataaaaaact tatatcggaa gctgacttaa gaaaaccaac tataaatgga
    22441 gttaaaaaca tattagttaa taaaaaaagt ataataacac cattagctgt agattttatg
    22501 agaatacacc atctcaaact aaagaaagtg taaggaggta attttatgga tataggaaaa
    22561 gttgtaggta atgtttgggc tacaagaaaa gatgaaaaat tatgtggcca aaaactttta
    22621 gttgtaaaaa ttttaaaaac taaagacaca tacaaggaag gcttcatggt tgcagccgat
    22681 aatgcaggag caggaaatgg agacttagtt cttatatcca aaggttcctc agctagagag
    22741 tcgattgaaa aatctcatgt tccggtagat gcaactatag ttggagtagt cgattcgttt
    22801 gaggtgtttg atgaataaaa gtataggagc tatagagttt aaaagcattg ctaaaggtat
    22861 agaagtatcc aatgaaatga taaagaaatc ttcagttgat gtattgtact taaaaagtat
    22921 ttgcccaggt aaatttctaa taatagtagg tggagaaact tcttatataa atgaatgtgt
    22981 agattatgga ataaaacttg gagaaggata tattgtagat aacttcgtaa taaatgcaat
    23041 ttcacaagaa atattagatg gatttaaaaa taagtatcaa aagctagata gtatagtctc
    23101 tataggcgtt gttgaaagta gtaaagtatg cgctggaata aaaatgcttg ataagacttt
    23161 aaaatctggc gacttagtat tagtaaaact tcaattatct tttgctatag gtggcaaatt
    23221 agtatatata gtagcaggag atttaagtag tttagaatat gcattaaaag aaagtgaaaa
    23281 tgtagtaaga gaaaaagaag taatatataa gactgtaata ccatcagtag acagtcaaat
    23341 aataaaaagt ctaataaaat aatgggggga attatgagta taaacgaaat tataatctat
    23401 gtaatggtag tcttcatggt acttggagct atagataaat gtataggtaa caaatttggt
    23461 cttggagaac aatttgaaga aggtataatg gcaatggggt cacttgcagt tgcaatggtt
    23521 ggtgttatat gtttagcacc agttcttgct gatgtattaa gaccaattgt agtaccagta
    23581 tttaatatac ttggagctga cccagctatg tttgctggaa gtttattagc taatgatatg
    23641 ggtggtgcac cacttgcact tgaacttgcg aaagacccta atgctgggtt atttggtgga
    23701 ttaatagtag gggctatgat gggacctaca atagtattta taataccagt tgcgcttgga
    23761 ataattgaaa aagaagacca aaaattctta gcaacaggaa tacttgcagg tataataaca
    23821 atacctattg gagcattcgt tggtggatta gttgcaggat tcgaggtaat gatggttctt
    23881 agaaacttag tgccaataat aatatttgca gtagttatag cacttggatt attaaaattc
    23941 gaaaatgcta tgataaaagg attcacatac tttggaaaag gtgtagtaat agttataact
    24001 ttaggacttg ctgctgccat agttgaagct ttaacaggaa tagttgtaat acctggaatg
    24061 aaaccaatca gtgaaggtat agaaatagtt ggagatatag caatagtttt agcaggagca
    24121 ttcccattag tgtttgttat aacaaaggta tttaataaac cattaatggc tttaggaaaa
    24181 gttcttggaa tgaatgatgt atcagcagca ggccttgttg caagtttagc taactgtata
    24241 ccaatgtttg gtatgatgaa agatatggac aatagaggaa aaataataaa tgtagcattc
    24301 tctgtatcag cagcattcgt atttggagac cacttaggat ttacggcagg atttaattct
    24361 aatatgataa caccaatgat agttgctaaa ttagttggtg gtataacagc agtattgtta
    24421 gctatggtta tcgcaaataa gacactaaag aaggagaatg tataagatgg atatatcaaa
    24481 tatagataaa aatttaattg aaacattagt aagacaaata atagaagaaa aaataagtgg
    24541 aactaaagac acagtagatt ttgttagaaa taaagacatt agtggaataa cttctataaa
    24601 gttaccaact gtaaaagtta gtgaatctga tagattagat acagggaatc caagtgatgt
    24661 agtttacaca aaagatttat ttacattaga ggaaagccca agattaggtt gtggtatgat
    24721 ggaaatgaaa gaaacaactt ttgattggac tctaaactat gatgaaatag attatgtaat
    24781 agatggaact ttagatataa taatagatgg aagaaaggtt tctgcttctt caggtgaatt
    24841 aatatttata ccaaaaggaa gtaagataca attttctgta cctgattatg ctagatttat
    24901 atatgtaaca tatccggcag attgggcatc tcaaaattaa taaaaatcaa gttaagttaa
    24961 taataatcaa ataaataatt taaaatacaa actcttaaat aagcacacat atttaaaaaa
    25021 gctatgaatt aaaattcata gcttttttta taaagaatgt tatattttag acgtattttt
    25081 tctatattta ctgggagaca tattatattt tttattaaat aatctaataa aatgctctac
    25141 atttggataa ccaatactat tttactgata atcttttcct tatcactaat cttacatttt
    25201 aaaatattat ttttactcgt aatattatca taagaatttg tttcttattt aaaactaatt
    25261 agaattataa agtttattgc catgttatta ataaaataaa tccagttaaa tcgagtgcta
    25321 gttaaagtaa taagaaactt ttacaatggt ttcctttatc gattgaaata aaagttaata
    25381 tggtataata tctaaagaga gaatcttatc aattagaata tgcatagaaa caagtggctt
    25441 aaaaggagac gttatatgga agagatagta aagaaggaac aagcaagtat gaatgatatt
    25501 tttttgtatc ttaaagctaa atcaaactca ttatataaat ttgtaatatt atataatgag
    25561 tttatcaata aacctaaaga ttatggaact ggggaactta taaacatggt tgaagtacat
    25621 atgctaacag cgattgaaga aaatccagga ataagtccaa ctcagttagc taaaatatgg
    25681 aacagaacta aaggtgcaat atctcaaact atatctaaat tggagaaaaa gggttatata
    25741 actaaagaaa agaaagaagg aaatgcaaaa actatattac tatatccaac cacatcagga
    25801 gtagagttaa gtaaggcaca taaactatat gacaccatag atgttacaag aacatttgaa
    25861 tacttaatca aggagtgtac attagaggag atagatagtt tttataaagt aattgatttg
    25921 tttattaatt tattagaaga atctccgcag gaaaataatg attctgaaaa agtgtagctg
    25981 agattgaagt tgaataaaga gaaatagat
//
LOCUS       DAELTA010000041        24902 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.041, whole genome shotgun sequence.
ACCESSION   DAELTA010000041 DAELTA010000000
VERSION     DAELTA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.041"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            22..696
                     /locus_tag="KPG13_001481"
     CDS             22..696
                     /locus_tag="KPG13_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985216.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            684..1844
                     /locus_tag="KPG13_001482"
     CDS             684..1844
                     /locus_tag="KPG13_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0985217.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2060..2587
                     /locus_tag="KPG13_001483"
     CDS             2060..2587
                     /locus_tag="KPG13_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG0985218.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2873..3541
                     /locus_tag="KPG13_001484"
     CDS             2873..3541
                     /locus_tag="KPG13_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985219.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3544..6012
                     /locus_tag="KPG13_001485"
     CDS             3544..6012
                     /locus_tag="KPG13_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985220.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6252..7676)
                     /locus_tag="KPG13_001486"
     CDS             complement(6252..7676)
                     /locus_tag="KPG13_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985221.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7695..8387)
                     /locus_tag="KPG13_001487"
     CDS             complement(7695..8387)
                     /locus_tag="KPG13_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985222.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8439..9218)
                     /locus_tag="KPG13_001488"
     CDS             complement(8439..9218)
                     /locus_tag="KPG13_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG0985223.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9215..9985)
                     /locus_tag="KPG13_001489"
     CDS             complement(9215..9985)
                     /locus_tag="KPG13_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG0985224.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(9982..10686)
                     /locus_tag="KPG13_001490"
     CDS             complement(9982..10686)
                     /locus_tag="KPG13_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0985225.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(10941..11453)
                     /locus_tag="KPG13_001491"
     CDS             complement(10941..11453)
                     /locus_tag="KPG13_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985226.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11779..12036
                     /locus_tag="KPG13_001492"
     CDS             11779..12036
                     /locus_tag="KPG13_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985227.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12179..12805
                     /locus_tag="KPG13_001493"
     CDS             12179..12805
                     /locus_tag="KPG13_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG0985228.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13052..13831)
                     /locus_tag="KPG13_001494"
     CDS             complement(13052..13831)
                     /locus_tag="KPG13_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0985229.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(13899..14360)
                     /locus_tag="KPG13_001495"
     CDS             complement(13899..14360)
                     /locus_tag="KPG13_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0985230.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14336..15013)
                     /locus_tag="KPG13_001496"
     CDS             complement(14336..15013)
                     /locus_tag="KPG13_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0985231.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15246..15632
                     /locus_tag="KPG13_001497"
     CDS             15246..15632
                     /locus_tag="KPG13_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0985232.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15635..17434
                     /locus_tag="KPG13_001498"
     CDS             15635..17434
                     /locus_tag="KPG13_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG0985233.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17789..19663)
                     /locus_tag="KPG13_001499"
     CDS             complement(17789..19663)
                     /locus_tag="KPG13_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0985234.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19668..21332)
                     /locus_tag="KPG13_001500"
     CDS             complement(19668..21332)
                     /locus_tag="KPG13_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG0985235.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21346..22167)
                     /locus_tag="KPG13_001501"
     CDS             complement(21346..22167)
                     /locus_tag="KPG13_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0985236.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22266..23030)
                     /locus_tag="KPG13_001502"
     CDS             complement(22266..23030)
                     /locus_tag="KPG13_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0985237.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23027..23776)
                     /locus_tag="KPG13_001503"
     CDS             complement(23027..23776)
                     /locus_tag="KPG13_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0985238.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24280..24885)
                     /locus_tag="KPG13_001504"
     CDS             complement(24280..24885)
                     /locus_tag="KPG13_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0985239.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
BASE COUNT         8907 a         4024 c         3048 g         8923 t
ORIGIN      
        1 aatttagaag ggagcattct tatgaacaat aaaatactcg tcgtagatga tgaggaaggt
       61 ataataacac tcttaaaaga ttactttgaa atgaatggtt atgaggttta cactgctcaa
      121 agtggaaaag acgcattaaa aaaaatatct aaaaatccag atatcattct acttgatata
      181 aacatgcctg aaatagatgg aattgaagtt tgtaatagaa ttagagatta tgtatcatgc
      241 cctattctat ttcttacagc tagaattgaa aatactgaca agataaatgg ttttcgtgct
      301 ggtgctgatg attatattgt aaaacctttt gatttagacg aacttggagc tagagtatct
      361 gcccatttaa gacgtgaaca aaggaagtca aatcaaacta aaattaaatt ttataatgat
      421 ttaacaatag attacgaaaa aaggttaatt tgtatatata ataatcagat ttcactttca
      481 aaaaaggaat ttgatatagt agagcttctc tctatgaatc ctggacaagt atttgataaa
      541 gaaaaaatat atgatagagt ctggggatac aatagcaatg gaaatagtga tgttataatg
      601 gaacacatta gaaaaatacg aatcaaacta tcaaaacata ctcttgaaaa ttatatagaa
      661 acagtctggg gagttggtta taaatggata aaataaataa tatgtcatta aaaaaagctt
      721 tctttgttct tactttatct tcactaatta tagcatcagt gcttactact ttcgcataca
      781 ttttacttaa taatctatat aattctatac aagataagta tttaaaaaca cctataaata
      841 caggtattat aaccatagtt caaaacaatg tgaaccaatt tagtgattat gataaaacat
      901 tacttaatat aatcaacata cttcagcttg tacttccact tatctttttt ataggacttt
      961 tattattagc tgatataatt ttttacaagg taaaattaaa aacacccata gaaatactca
     1021 ataaaggtgc aatggaaata tccaacaaca atttagactt ttgcttagaa tataataata
     1081 atgatgaatt aggtaatcta tgtaatgctt ttgaaaaaat gaggtctgaa ctaaataaaa
     1141 acaacataaa gatgtggact atgattaatg atagaaaaca acttaatgct gctttttctc
     1201 atgatttaag aaatccccta acagttctaa aaggttactc aaactattta acaaaataca
     1261 taccaacagg taagcttagt gatgagaaga ttttatcaac cacacaactt atgtctgagc
     1321 atattaacag aattgaatac tatgtagaaa atatgagtaa tgctcaacga ttagaagatt
     1381 tagttgtttc aaaatctatg tcgaatataa acaagtttat agaaaactta gatgaaaata
     1441 tatctatcat tgctaaacaa gaaggaaaat cttttacact taaaagtcaa ataaataata
     1501 taaatctatt ttttgatgaa aatatcattc ttagagtcgt cgaaaatata atatcgaatg
     1561 cttttagata tgctagaaat aatgtatcca tacttatata tcttgaacaa gaactcctta
     1621 cctttgtaat tgaagatgat ggtataggat ttagtaagga atctctaaaa ttagctttaa
     1681 gacctttcta tagagataag actttaaatg acagcaatgc acactttggt atgggtcttt
     1741 atatctcaaa aatattatgc gaaaaacatg gtggctctat ttctattgaa aataattcta
     1801 ctaacagtgc taaaattact gctaaattct caacaagaaa ttaaaatgat tttgtaagct
     1861 ttcactgttc ttatataaaa tttgttagtt tagaatttat aaaatgtatt tcaatttaca
     1921 accaatatat atactatttt gtagaagttg taagagttgc ttcaattata atatttggca
     1981 aaaggcattt gaaataataa tagaaatata tatttaaata tgtagaatag tatgaaatta
     2041 gaattttgga ggtgattata ttaaaagttt tgctaataat attacattct ctcgtattgt
     2101 gtttgcattg ctaatgctta gttctacatt ctcttcactg cctttctgga tatgttattt
     2161 ttggtgtgga attagtgata tacttgatgg tcttattgcc cgaaagttga gacaacaaag
     2221 tggaataggt gcaaagttag atagtattgc tgatttcatt tttgcggtta caattttggt
     2281 tactgtcgga aaaaatattg ctcttcccat gtatctatgg tggtgtattg ttgcaatagc
     2341 attgttacgg ttgattagtt acagtattgg tttttttaag tatcgcacat tttcatcgct
     2401 tcatactttg atgaataaaa tcactggaat ttctatcttt gcttttccat tactctatat
     2461 ttgctttggt cttgatgttg caggaattat gatatgtatc gtggcttttc tttcttcttt
     2521 tgaagaaata gcaattatga taaaatcaaa agaattaaat cgtgatataa aaagtatatt
     2581 tatgtgacaa cttagaattt gtgacaaaaa taatttatat aataatgagg aagtaaaaca
     2641 taaaaaatta tccacaagtt tatgtgtaaa atagttttaa agcaatagag ttgtattgta
     2701 aagcctttag tgcataaatt acattttaaa ttaaaaatga tactgaaact gcttatcagc
     2761 actgcgaatt atccgcaaat agtgaagact ttttagattt aagctaaaag ttgggaaaaa
     2821 attgagtttt aaaacttaca ctcttcttat atagaaaagg agagtgtttt ttatgattat
     2881 aaaagcaaaa caattatcaa aaatatatgg ttctaataac aataaagtta tagcacttaa
     2941 taatgtaaat ttagaaataa actcaggtga atttgtttct gtaattggac cttctggaag
     3001 tggaaaaagc acacttttgc atattttaag tggattagat aatcctacct ctggacaagt
     3061 gttacttgat gataaagata tatataaaca cactgaaaaa gaactatctg cacttagaag
     3121 aaaatccttt ggattcgtct ttcaacaatt taatctttta cctgttctta cagcatctga
     3181 aaatatttca atgcctgtac ttctagataa aaaacaacct gataaagact atttaaatga
     3241 aatttcatca ttacttggaa ttgcagatag actcaatcat cttccccatg aactttcagg
     3301 cggtcagcaa caaagagttg caattgctag agcacttatt gcaaaaccag atataatatt
     3361 tgctgatgag cctacaggaa acttggacag taaaagtggc agtgaagtta tgaatcttct
     3421 tataaaaact tcaaaacagt ttggaaagac tttagtggta attactcatg atgatagaat
     3481 tgcaaaactt gctgatagaa aaatttcaat tattgatggt gttcttatgg aggtgaaata
     3541 gaaatgaaga attacctttc actttcaata aaagaattaa aaagccagaa atttatgact
     3601 acattcatca ttatagcaat agtactatca actattatga caactgtggt tggacagtca
     3661 attggtatcc tacaaaattt aagaattgaa caagcccgtt cttttaatgg tgatagacat
     3721 gtatcctttc accaactcac taaaaatcaa gttgatgaca taaagaaaga tgatagagtt
     3781 tatcaagctg gtacttcaat aactattggc agtagtaaaa taaaggattc tggtatctct
     3841 gtacttgtaa aagagtatga taaaacagga ctttctaact acccaaaatt aatgaaatta
     3901 aaatctggac atttgcctaa agataaaaac gaaatagcct tagatgaaaa cacactaaag
     3961 cttatgggta taaaaccaag actaggagtg actataccta tgaatttaga tatttcactt
     4021 ttaaatgata ccataccacc atataattac acagctaact ttaaattgag tggcatttta
     4081 gaggatgatt atacaggata tgtaagtggt atagtaaatg gtattgtagg taaaggtaca
     4141 tcagaaaatc tattacctaa gagatatatt ttatcctctt tggattttaa aataaaacag
     4201 caagaaaagt ttcaagaaat agtaaatcaa ttagctaaaa aaataaatct ttctcacaat
     4261 tcaattcaat ataattggat ttacttaaat gctcttaaga tacagtttga aaaagatgaa
     4321 aatagttcta atagtgatgg tatatctatg attatattgg tatctttatt tgttgcctta
     4381 ttggttttac tggcttctgg acttgtaatt tataatattt taaaaatctc tgttacaaag
     4441 aaaattaaag agtatggatg tttaagagca ataggagctg aaccaaacca gatatataag
     4501 atagttattt tacaaatctt aattttatgt actgttgcca tacctattgg agctgtaatt
     4561 ggaattatat cttctaaagg cataactgga atggtcacta atatcttaaa tccagatata
     4621 cttctagcaa atgataataa agaaattact gagcttatac ataaaaatac aacagcatat
     4681 atattcccat tagtattaag cactaatgta tctttgattt tttcatttat ttcagcatta
     4741 ccatcagcaa tatatgcttc acatgtctct cccaaaattg ctatggttgg tagtactacc
     4801 aaaatcaaaa gaaaaattaa aagagaaaaa acaataaaaa actttgaaag acatctagca
     4861 tggctaaact taaaaagaaa taaaggaaga actataataa ctatactatc attatttatg
     4921 agtataacag tattcgttgc actgagtgag ttctctaatg tattagatgt atctaggtca
     4981 gtaagtaatt taaaagaagg agatttttcc ttaacaaatg aaatatctgg ttttgataaa
     5041 tcaacactag ataaaatagc taaaatgaaa aacgtcaatc gcacttcctt tataaagtac
     5101 tctgaataca aacagggtga aatagataca gatataaatt ttgaaaatag tggtgagatg
     5161 ttaaaaatca ttggaattga tgagcaaact ttaaaagact taatgccttc tattacagat
     5221 tctatactag aagactttaa aaatggttct atctgtttta taaaaaatcc gattgctatt
     5281 tctacaccag gtgtaaaaac tagatataca aatttaaaac ctaaagatag tatcacaatc
     5341 aacaaaaaac aactagatat atatagtaca gtagataaaa tgttcttttt acaaggcaat
     5401 ggctggataa atggtgttga tgttattgta tatgattctg tttataacac tttgacaaat
     5461 aaaaacaagt tcaaccagat aaatatttat gctaaagata aatcaaaatt agaacaaata
     5521 agattgtcta ttgaacagat ttgtgaaaat aatcctggct ctcattggat atcatatatt
     5581 gaatcagata aacaacttaa agagagtttt aaacaaatag aatttctagc atgggcagtt
     5641 atattatttg tgggattgat tggtacttta aacatcatta acacaactca tacaaatata
     5701 aatactagga ctaatgagat tggagttaaa agagctattg gtatgagtaa taggagctta
     5761 tataaaatgt ttttatggga aggtgtttat tatggaattt ttgctgctat atttggttca
     5821 attgctgggt atgccagtgc tattatcata aacatggcaa ctatagaaaa gcttgatttc
     5881 actaatatac caattacttc aattttacaa gctactatta tttctgtact tgcatgtatt
     5941 atagctactc ttattccgtt aagaaaagtt aaaaagatga atataattga ctgtatagat
     6001 atcaatttat aactatttta cagataataa tataaattaa attatcaatg actgtctcta
     6061 ttttctttca aatctaacta ataaatcttt ttaactataa tataagcttt aaaaaaggcc
     6121 tgtctcaaaa tagagataag ccttttttca ttgtcgaaat aatgaaaata tatttaactt
     6181 cacaaaaaaa gagtgcctca cgaattaaaa atttcgcttt gagacactct cctttaattt
     6241 aaaatatata gttaaatgct atgccaaaat ttcacacatg ctccacctga ttttgaattt
     6301 tctattttaa cagaacctcc aagctgttcc actaattgtt tgacaatgta aagcccaaga
     6361 ccagaatgtc caccttttgt ctgcctagct tcatctcctc gataaaattt atcaagagcc
     6421 ttttttaaat ctttagagct aaaccctcta ccagaatcac aaatctcata agaaatacaa
     6481 tttttttcat cctttacagt aatactaata ttaccacctg atggagtgta ttgcagactg
     6541 ttggatatga tattatcaaa aattcgtgtc aacctatcaa cgtcgatttg aattggggat
     6601 tgtatattcc cctgcatttt caatataagt tcaacttcct tttgatgagc ctgtaattca
     6661 tagtcttgca ccttttgcct caaaaattca ggtaaattta taggaacaag atttaattgt
     6721 atatttgact tttctaagtc agacgtatat tgcatctttt ggacaagagc agcacttttt
     6781 tctgtatttt ctcttatcac tgtaagatac ctatgaagtt tttcattgtc atctgtatta
     6841 ttaccaataa gtgcatctgt atatcccaaa ataatagaaa gaggagattt taaatcatgt
     6901 gctagagctt ccaccatttc aacacgttcc tgctccatct tccactgtgc agataaagag
     6961 ccttttaatt cgtctttcat ttcagaaaag gcactacaaa gtttaccgag ttcattatct
     7021 gaataatagt cgatttcaaa atcaaggtcc ttttctttta tcttcttgga cgcatcaatc
     7081 aatagatgta atggttgatt gatattcttt acaaagcgct tagaaagcca tctagtaaaa
     7141 cccacgatat atagaaatgg agaaaacaaa gccattataa taactgcaaa tacaaatctc
     7201 cctctaatat ttgcaaaggt gattttcact tggtagaata gaataacagc accctcaatc
     7261 tttccattat cccctttgat tggaacagta tggatataac cacctttacg aacagtttta
     7321 ttgataaaat tattaaataa ttcttctttt gttttaaata gttttttggg gttagtacca
     7381 tataaaatat tgccattatt atccacaact tgatatagca tatcatcccc acgaatcgtt
     7441 ctttttaaac cttcttcatt cgattgtgat aacaaagcta tgtttttttc attgatgtat
     7501 ttttcaattc taggaacttg tcgctcataa tagtttggtg gatagatatc cttatttaag
     7561 gaatgaatat acaaatagag agcaaataca taagtaatca ctgtggcaac tatactggct
     7621 attataataa aaataaaagt atgtctaaat tgggtaataa gggattgatt tttcatttaa
     7681 taacctctct ctctttacat tttattccac ttatacccaa taccccaaac tgtggaaata
     7741 tattcttttt ctggtgtcac agcagaaaac ttagctcgta ttttttttac tcgttcaaca
     7801 acagttgagg aatcaccttc agaatcacac ccccatactt tttcatagat ttgttctctt
     7861 gagaaaactt gtcctgcatg gagtgctagt aattcaacaa tatcatactc tatttttgtt
     7921 aaatcaatat tcttaccatc aattttaatt gcacgttcct tcatatataa gcataacttc
     7981 ccaaaataca aagtagatct cttgttctct tcattgatat actgtgaacg cttttcccta
     8041 cgcagattag cttcaatcct tgccataagt tcacgcagtc caaatggctt tgttacataa
     8101 tcatcccctc caaaggtcaa ccctttgatt ttgtcggttt ctgattgctt tgcacttaaa
     8161 aatataattg ggcataaaac atcatctcta attgcctgac atacctcaaa tccattcatt
     8221 ttaggcatca taatatccag tataataagg tcaggctcct gttttgataa ctctatacct
     8281 gcctgaccat cataagctat caacacttca tgtccttttg ccttcaattc atcctcaagt
     8341 agcattacta ggtccatttc atcatcaata atcaatattt tactcaatat aacaccacct
     8401 ttctcttatg ggaaggtcag tattaaaaca atattcgtct actcataact tttccttccc
     8461 tcccacattt taaaccaaat aacgcctcct atcaagaata taatcaaacc tattgctact
     8521 actataagct ctatactagc tgtttgcatc actttagagt aaaatgcctc agttgaaata
     8581 gttccagctt ctttaataaa ataaattcct ggaagcatac ctagttttac tggccatgtc
     8641 catggaacaa atgcccatac tttttcacca acgctagtta atccaagtat tgctgccatc
     8701 aaaattccac caatactaat accaatagat gcacccatac cccatgcaaa actcacccaa
     8761 agatgaatag ctaatagtgg aagtgtccca atcaccacaa gtcctgctgc tgacaggaat
     8821 aaccatattg ttgctccaga tggcactgca atactcattc ctatgcaaag tatcaacgta
     8881 gcaataaatg tgcatatagt taggcaaaac agaagaaaaa gaaattttcc cagatataat
     8941 ctgacacgag aaatacctac acataaaaaa ccattaaaat ttccagcaag ctcttcctcc
     9001 tgcactataa atcctgctaa aatacctact ccaataggaa tgatgaatac agtccatact
     9061 gtaaaaaatc cttcaaaagc aaattcttgt gtacttccag atttagttgt gagataaaaa
     9121 accacacata atgaaaaaat aataggcata caaagagtaa gccactggac agctgtcctc
     9181 tttgctcgaa gccattctga tgataatagt tttatcattt gtcatccctc ctattgaacc
     9241 aagctgcagt aagtgctgtt aatactataa acacaaccaa agaaacaaca atcccaatag
     9301 gaatcactga agtattcaaa agtgggtctc cagcttctaa tatagtaccg ttaggatgta
     9361 tgttgatgat tgggcatacc attctaactg cccaactcca aggtactgca atccaaaatg
     9421 gttttgtcgc agcaagaaca ccaaatatca tacctaaagc tccaactccc atacttagga
     9481 acataccttt ccatgtagct agccatagct gaagtggaag tatagcaaga gaaacaaccc
     9541 agcatacaat acttgcccct ataatctgtc caaaaggaac tggaccagct ttcaatatta
     9601 aaccagttat aattgttacg actattaaaa ctagcgttga aataaaactg tagactgcca
     9661 tagctataac tttattaatc caaagtgtca taggagacac attgtgtgta cgtaaagcac
     9721 gataatttcc agcttttttt tcctggaaag ctaccaaagt agcgaacaat gcaaatccaa
     9781 taggcagaaa taaaaatgac caccagttaa aaattaatgc taaaagattt tgccacgccc
     9841 aagaagttgt ttgagataat ggattttgca tgagtgttga ttgcattata aatgcatatg
     9901 ctgcaaaaga cactgggaaa aaaacaataa gtttacccat aaatgttctt ttatacttta
     9961 aaagttctga ttgtaaagca ttcatctgtt ttgtcctcct ttaagaataa catctgtaaa
    10021 aaattcttct agattttcat tagggtcagg cattccttga aataatagtc gtcctccatt
    10081 tataattcct atattatcta caacttgagc aacctctgat agaatatggc tagaaagaat
    10141 tacagttata ccttttttgg gaaaagaagc aatcaattca cgcaattctt gaatgccaaa
    10201 ggggtcaaga ccattggttg gttcatctag tattaacaac tctgggtcac tcaaaagtgc
    10261 aatagctatt cctaaccttt gtttcatacc catagaaaat tgtgaagctc tctttttacc
    10321 tgtatttttc aaatttacta tttccaacac ttcataaatt ttatctttag gtattcccaa
    10381 tatttttgta tgtactaaca aattttcctc tgctgtaagg tttccataca atgcagggga
    10441 ttctattaaa gaacctattt ttgcaagaga ttctcgtttc caaggctttc tatcaaaaat
    10501 aatacgacca tttgttgggc gaagaagacc aacaagcatt ttaagtgttg tggactttcc
    10561 tgctccattt ggtcctaaaa gtccatatat actatttcgt tcaatttgta gagaaacatt
    10621 gttaactgcc atttgttctc catatctttt tgataagttt tttgtttcaa gaattaaatc
    10681 tttcatatta atttctccct tcttgtaata ccttaaatct gttactaaga gaatactaac
    10741 atgaaaaaat aacaattcta taacacctac taattttaat aaaatttaaa ataaaaaata
    10801 gagttaccaa ttttattttg gcaactctat ttaagtttat gttttacagt tatattagat
    10861 ttaaacttag tttcattatc acactaactt caaatttact ctataatctt taaatcaaac
    10921 actaatttat taatcaaaca ctaatctatt aatttatatt tctttagaca tctaatatag
    10981 tttgctacta aataataatt atttatatca tatttatcta atcctttaaa agaagttatt
    11041 tcatcattaa gctcatcact accttctaca aaaacacaac ctgtttcaaa ttccatacct
    11101 acaataatct tacagtcact ataaatttcc tcttgagtat cgtacaattt ttttgcattt
    11161 ccttcatttg gaattgtaaa tacaataggt tttaattctt tatcactgga aatcttttgt
    11221 gaaaaaggct tgtttaactt aatattattg ataactacat gtttaaattc ctctgaagtt
    11281 tcttctacta ttacatattt tcgctttaaa aactctatta tttctaaaac agacttagaa
    11341 tttactttta aaatactttt atatttttca tataaagatt tccactgttc aattttttcc
    11401 tgtggtattt cttggatatg tgtacttatt ttttcttgag atattttttt caattaaact
    11461 ctcctttcaa tacttaaata tccttatgaa tctcacatga ctaaagttac aaggtttcta
    11521 cttaatagaa ttttgcatac ttaaatatgt cttattaatt ctctacagga tatctccgta
    11581 gttcctacga ttcttacata agttacttat tttaaattgt tattagttat attctaaaaa
    11641 ttatttattt tatcactaat ataatactct taaaaacttt acaaatcaat ttaaatttgc
    11701 taccttttat ttataacaac ttatagtaca tttctcctat gcttttcaat atattacttt
    11761 taaaaggagg ttttcattat gattttaaat agagctgagc ttttccgagg atattcttta
    11821 aatagattta acgagatatg caatatatta tcagtaaata aaattaaata taagtataaa
    11881 gtgaaaaata atgcttactc taaaaaacct ttttttaatg aaataacact tggcaattta
    11941 ggccaaaagg aagatttctc ttatgagtac ttaatttatg ttcataaaga cgattatgaa
    12001 caggcagagt ctctaataaa ttctaagttg cgatagatta tctataaaac atgacattct
    12061 gttgtcatgt tttatatgtt attatacttt caaatatgct actatatttt caaaattgtg
    12121 tgctttaaaa ctagcttttt ttagtaattt aaagcttaaa actaaggaga ctcgttatat
    12181 gaaaataaat ataaaatcaa agttaagtga atttattaaa cagaacaatc tttttgatga
    12241 cagcagaata atatcctatc ttcctaatat aacaatagaa acagacaaaa taaaaactat
    12301 aatgattaat gaggttgtcc caactaacac caatgacgat ttttatagtg tagataaaga
    12361 tgctgattat ttaaaaacta ctattccttt atttgacagt gcaggaataa aagtatctaa
    12421 cataaatgat atcctagata tgggtatcta tataactaat gcagtaaaac ttcctaaatc
    12481 agaatataca ataactcgtg atacaattaa attacatatg cctattttag aagaagaaat
    12541 taaattattt aaaaacctag aggtagtaat gttaatggga gatgttgcaa aaaaatcttt
    12601 taatatgatt actaaaaagc atataaaaaa gaatgtcatc ccttccattt caacatataa
    12661 acttcgtaat aatgaacttt attatgataa tttaagagta tttccatcat atattatgac
    12721 tggtggaaac atactgattg aaaaatcaaa atttgaaatg gcatcagaag atataaaaaa
    12781 aatgttcgag attataaacg gttgatatta caaaataata aaaactaata taaaataaaa
    12841 agtgcctcaa aaacttctat ttatgaggca cttttatttt atattagtgt aactatatac
    12901 tattaacata atttattatt tatattgttc tttttaatct ctattccatt tagtctaggt
    12961 aacattactc tttacaagat tactttatat ttcttttata tctctaacgt ttttatttaa
    13021 taaaacaatt attaatacaa gtcaaatata tctatttatc taaaaatata tcttttacag
    13081 taatatctgg atggtgatac ttcttcctat tcgttaaaaa agattttttt acattaatag
    13141 cctcttttgg acaccagtga aaacaagcta ggcatctttc acactcatta ccccactttg
    13201 gctttttatc aactatactt atattctcta caggacaaac ccttttacat gtcccacatc
    13261 cattacattt attttctgca aaaaatttag tagttgcaat ttgcatattc ttacctacta
    13321 aacgaagtac aacactatct atatgtgact ttaaattgtt ttttccttct atatttccaa
    13381 tctccaagtt attgatacat tttgcaatct cagctaccct attagtctga tttttcaatc
    13441 tttcttcttc aacatctgga ggtgttatca aagcatttcc aggcatattg attaaaaaac
    13501 cagcagataa ttttttcccc ctttttttaa gtaacttatc tatagtaaat aaactatgtc
    13561 cagctacccc attacaagtg gcaatggcat atatatgagt attaatatta aaatttaatt
    13621 tctctacaaa agactttact atatcaggaa catcagcaaa ataaacagga aaaacaatta
    13681 ccaatatatc ttcatttatg tctatattat tttcttttct atgtatagct aagggtataa
    13741 cttctttact tccatttaac gtttctttta aaatatttga aactactagc gaatttcctg
    13801 ttccagaaaa ataataaatc ttactattca ttcttatccc tctttctata tttctacatc
    13861 atcaatagtt aaaaagtctc ttaaaatagg atttatattt actttccaat tctatccaaa
    13921 atatcagtaa acaaaggcaa ttcttcatca gagatagatt taaatgtctc tgttgcatat
    13981 atacttgcaa tttcatttaa tggttcttct aattctctac ctaaatctgt taggaatatt
    14041 tgaaacttac gtctatcttt aacatctact ttacggacaa ttaatccctt atttttcata
    14101 acatctaaaa ttcttgttgt attagattta tccttacatg atttttcaca taaatctatc
    14161 tgagaaatcc catcttgctc ccatagaaac cttaataacg cataatggtc agttgttata
    14221 tcatatccat tttgtttaaa catctttttt aaatcatctt ttacttttaa cgaagtactt
    14281 actaacttag ttgtagcttc atatagtttc tcaaaatctt tcattttacc tccatttaaa
    14341 taatcccttg ctcctcccat ctatcaatta ttcccttata ttgctcaata tctttaaaag
    14401 ccattggctt taaaacagca tttatagcaa taaaattata gatttgagaa agcacaggtg
    14461 gatttttctt ttcctctatg caataaataa attttttaga taccttttgt atctttgatt
    14521 ctactttttc agttatagga ttttgattaa atccagtttt tatagataaa ctaccacaaa
    14581 cataacctcc cgaatctaag accatggatt ttataaaaga aattgctttt gaaccacgtg
    14641 cattttcata tgtagtaact gctatacatg gcttacgata caataatctt tcaagcatca
    14701 tatgtgctct atctgtaaac accttgaaaa gtccagttac actacttcca taagttgggc
    14761 taccaaatat aacaccatca ctcttagtaa ttatatcatg aatatactct actttatcat
    14821 tttgattgat acacttgccc attttataac aatttaaaca accaatacaa tatgatggat
    14881 ttacctcact taaatcaaaa taattaattt ttacatctgg tattaatctt tctatatctt
    14941 tatacaagta agttaaaact tttgatgtag ctccattttt tctagggctt ccatttataa
    15001 ctgtaattat catttctata tctccttttt agttgttata tcaacaataa gtatatcaac
    15061 tataatttta gctgtcaata taaaccattt atttaaagct atacttacac agcttaaata
    15121 aaagttttat ttaactacat gtgcttattt aatcaactat aaatgaatta tttttaataa
    15181 ttgacaaact aaattaatga taatatactt tatgtattac aaatgtaagt agaaggaggt
    15241 atagtatgaa aaagttacca catatttcag aagcagaata tcaagttatg aaaattattt
    15301 ggaaatatgc tcctatcagc actactgaag taattgaaaa gttggtagaa acaagtacat
    15361 ggagtcctaa aacaattcaa acaatgttat tgagattagt taaaaaagga gctttaacat
    15421 atgaaaaaaa tagcagagta ttcgtttata ctccacttgt taaagaagaa gaatacgtag
    15481 ctacagaaag tagttctttt ttaaatcgtt tttataatgg tactcttaat tctatggtac
    15541 taaacttttt agaaaatgat aaactatctg aagatgatat tgaggaactg agagaaatct
    15601 taaataaaag gacgactaag ggggataaat aaatatgtat gccccattct ttatacgatt
    15661 tttactcagt acattaacct tatcaatatt aattgctgta attttacttg ccaaaaaagt
    15721 atttaaaaaa catatttctg caaagtacca atacaatatt ggatttatat tgcttataat
    15781 gttaataatt ccatttgtcc ctcttaaata tattaatctt ggaaatgtat ttaactattt
    15841 atatactttt aatcaacttg gaagcagtac taataggact tctatgataa aaaatagtcc
    15901 aaattctata attaataact caaatgcatt aaacgatttt tcagtatccc taaaccaagt
    15961 taattttgat agtttaaata ttttattact aatatcatgg ataattggaa ttttaatcat
    16021 gatagctatt actattcatt gcaacattaa aataaaaaat atgaaaaaat caatacaaat
    16081 tttaagagat gaggagatag tatttatatt tgaaaagtgt aaaaaagata ttggtataaa
    16141 taaggatttt atactatgca aatcacctat tataaataca cctattacat ttggatactt
    16201 taaaccacac attatattac cagataagtc tatatctaaa ttatctttaa aagatataaa
    16261 atatatctta cttcatgaat tacaacactt taaaaataaa gatatactga taaattatat
    16321 aatgtgcttc cttcaaatat tatattggtt taatcctctt gtatggtatg catttaaaga
    16381 aatgcgtata gaccgtgaaa ttgcatgtga tatttcagtt ttaaaaaagc ttgataaaga
    16441 tagtcatata gaatatggac aaactctaat aaactttgtg gataatattt caagaccatc
    16501 aaatcttaca cttacatcag atattggtgg gtcaaaaaaa caaataaaaa agcgactatt
    16561 gaagataatg gattttcata atgaatcaaa tacgttaaaa ttcaaaagca ttttaatttt
    16621 tacattgata gctttaattg tatttggaaa tattcccaaa ctatctatta tggcagatat
    16681 caatgaaaaa tatagaccta caaatgaaca aactgtgtac gaggacttaa gtcagtattt
    16741 taaaggtttt gatggaagtt ttgttatgta tgatttaaaa tccaagcaat atcatattta
    16801 caataaagaa aaaagtaaaa caagaatttc tccaaattct acttataaga tttatagtgc
    16861 cttattggga ttggaaaatg ggataataac aagaaataat tcacatatcc cttgggatgg
    16921 aaagcatcat tctaatagtt cttggaatga agaccaagac ttattttctg ctatggaaaa
    16981 ctccgtaaac tggtactttc agactctaga taaaaaaaca gggctaaaaa acatacaaac
    17041 ttatcttagg caaattggat atggcaatta tgatgtttca ggtggtatct caaacttttg
    17101 gctagaatct tcattaaaaa tatcacctat tgaacaggta gaattacttg aaagttttta
    17161 tacaaataag tttaaatttg aagataaaaa tatagaaaca gtaaaagatt cattgttaat
    17221 atcaaaaaag gatggggtat cactttttgg taaaacaggc actgccaata taaatggaaa
    17281 agatgtaaat ggttggttta taggttatgt ggaaaaaaat gaaaatactt atttctttgc
    17341 tacaaatatt caaagcaaag attattctaa tggtagttca gcatctaaga taacattatc
    17401 catattaaat gacaaaaaaa tctataatcc ttaatatatt tactgtacat tctaaaaaat
    17461 tcacataatt ttcatattaa ttatcaatta aattcatata caaggcttat tatataaaca
    17521 tagaagatag taaaactagc ttaaaaacat aatataatac cctatacaaa tagtggcaac
    17581 tctccctcac atcctcaatt gttgccacta tttaattttt tgtatgaagt taaatatatt
    17641 ttcatcattt caacaatttg ggtgcaatac aatctctatt ttgaaacaac ctctttaatt
    17701 gttttagagt ttatgaatta tttctcatac tttaaaaaga ttgaattact cttgataatc
    17761 ttcttttgta gtcattacat atcacctact actcaacaca tattctttga cctattttta
    17821 catccttgtt tatatccaaa atatttacct tcttaccatc tggaaacaat accattgtta
    17881 aagtagactt tcctaattca tgtattctct ttaaatcaac attaacaaga acatctccct
    17941 gtttaaccat atctccttgt ttaacagcaa tatcaaagcc atctccattt aatgcaactg
    18001 tatcaattcc aacatgaatt aatacttctc cattagttcc tttaatccca aatgcatgtc
    18061 cacttgaaac cactgtttct aatttacctg aaataggaga aacaattttt ccatctataa
    18121 tatcaatagc aaaaccatca cctaacattt ttttagaaaa tgtttcatct ggtacatttt
    18181 ctaatgcaac aattttcccc ttagctggag acacaaagtt ttcattattt tcattaaaca
    18241 ctaattcttc gtcttcatct tcatttatag cttctttagg cactccaaat aaacatgtta
    18301 caataaagca gaatgcaacc acaaatattg atgctaataa gtatgtgatt aaattatatc
    18361 catcatagat atacattaaa taagaaggta aaactgctaa tccatatgaa ttagattgaa
    18421 tatgtaaaat tgataatatt gctgctccta aacctgaaga tgcacacata ataatcaaag
    18481 gctttaaatt atatcgaagc tgaatcccaa ataaaacagg ctcacttata ccaaaaagtt
    18541 gagatagcat tgctccaact gcagtttgtt taacttgatt atttttagat tttttcataa
    18601 atgctagaca tgttccaata ttcgcaaatc catacattgc acaaagagta ataagggcat
    18661 taaatcctgt atttgctaat agtgatgttt caatcattac ataagtatga tgtaagccag
    18721 taataacagc taatggatat gtaaatccaa taataaatcc tccaattcct aaaggcaaat
    18781 caatcaataa gctgatgata tcaaccattt ttaattctat tgtatgcatt atagggccta
    18841 cacctaacaa aataactaac attgttatta gcataacaaa aaatggtgtg aaaataaggt
    18901 ctaatacatt aggcatatgt ttacggaacc atttttcaag attagctcca ataaatgctg
    18961 taacaatagc tgttaaaaca cttccttgac aacctacaat aggaataaat ccaaatgcca
    19021 taattgcttc aatttcattt gatgggtcag caacagaata tgcattagga agtattgggg
    19081 aaactaacat aagtccaata acaagtccaa taacaggtgt tcctccaaat actttaaatg
    19141 ctgaccatac aataattgct ggtaaaaaag caaatgctgt ttctgtaaga accgaaacaa
    19201 gtgtctgaat atacaatgga atatttgcag aagacattcc aaataatcca agtacattat
    19261 cattaaacaa acaacccttt aatcccaaga ataatcctgt tgctgcaata actggaataa
    19321 taggaacaaa tatatctgct agagttctca taactttctt aaatccagtt tcattatttt
    19381 taactaattc atcttgttct ttttttgata aagtttttaa tcccattttt tcaacttctg
    19441 cataaacttt attaactatt cctgttccta aaataatttg atactgtcca gaagtaaaga
    19501 aaaccccttt aacctcatca actttttcaa ctttcttatc attaataatt tctctatctt
    19561 taaccatcac tcttaatctt gtggcacaat gtgtaataga aataatatta tcttcaccaa
    19621 taattttaat aagctcattt gctattttat tatatttatt catttttcta cctcactttc
    19681 aataatactg catgaaatgg tttcaaacgc actgttttat ttaatagatt tatatcatct
    19741 aaattttgga atacaatatc atatttttct tctactctta tttctacagg tttatcagaa
    19801 aaattataat aacaatcaat aactaccaaa tctaattgcc ttctatacgc aataatatca
    19861 tcactttcta tatcaatagg ttctattgaa ccataaatca aacaatcaga atactcacta
    19921 tcttgtctca actttatcat ctttttataa aattctaaaa tactctctga attgttaatc
    19981 tgactctgtg cattaatatc tttataattt tcaataacct ttaaccatgg tgttgtatct
    20041 gaaaaacctg cattttcttt atctgcccat ggaaatggcg ttcttgcatt atctcggctt
    20101 cttaaattca caaattctaa cgcttcttct tctgagtaac cttcatctat tgaacgatag
    20161 tattggtcaa tgctagataa atcattaaaa tcatttatgg tatctctttt aaaatttgtc
    20221 attcctaatt cctgtccctg ataaataaat ggggttcctc ttaaaaagaa atacattgct
    20281 cccaacattt ttactgcatc atcattcctt tcgtattgca aaagatactt tgttgtagct
    20341 cttggttggt catgattttc aataaaatta gctgcccatc catgttcctg aacatacatt
    20401 tgactatcta aaatgcattt tcttaaatct ggaattgtcc atgaaattct tttaaaccac
    20461 tcactaccag aagcaacatc taagtcagca tgtttaaagt caaagaccat tgaaaagaaa
    20521 ccatcttctc caataaaatc acctaactgg tcatagctaa tacctgctgc ttcagcaaca
    20581 gtcatacaat catatttttt aaacgtattt tcttttaatt cataaagcat tttttctatt
    20641 ccaggctgat ttctaactgt ttttgtacat ttaacaagac catcaacacc atcactttct
    20701 aaactttgaa aacttaaatt ctttttgatg aatgtaatag catcaactct aaatcctgcc
    20761 actcccattt gagaccaggt attaatcatt tggtaaagtt cacttcttaa tattggattt
    20821 tcccaattaa ggtcaggttg tttttttcca aaagaatgaa aataatattc gtctctattc
    20881 tctacctttt cccaaacact tccaccaaaa acagacctcc agttatttgg caaatcgttt
    20941 ccttttttaa aaatataata atcatgataa ggactttctg gattttttaa tgcttcttga
    21001 aaccaaggat gttcatcact tgtatgatta agaactaaat ctaatataat tttgattcct
    21061 ctatctttac tttttttaat taactctttt aaatcttcca tacttccaaa ttcacttgca
    21121 atatccaaat aatcactaat atcataccca ttatcatcca taggtgattt aaaaattgga
    21181 cataaccaca atgatgtaat acctaaatct tttaaataat ctaaacgaga aataattccc
    21241 tgtaaatctc caattccatc attattacta tcttgaaagc ttcttggata aatttgataa
    21301 acgatttctt tttgccacca tttcttttcc ataaattact cctttttata tgatatttct
    21361 tctttttaat tctttataaa ttccattatc gaatatgtcc tcacatatag atgtcgctat
    21421 atctttaagt tgttgatgac tatttttcat tgcaattgta acgtctgatg cttgaaacat
    21481 gacaatgtca ttttgaccat caccaaaaca tatggtttct ttttgagtta ctcctaatct
    21541 ttcttgtaat ctcttaattg ctttgccctt atgaaaatct ttttgaataa tttcataata
    21601 ctgactagaa atatctctct gtgctaattc cattttatct tgtagtatat ctttcacttc
    21661 gtcaaatacc ttttcatttg accataaaca aattttatgt atatcttgtg acttatactc
    21721 ttcaatgtta ttttcatatg ttattttttc ttggatatgt tgtttattta tacaagaatt
    21781 agttcctttc aactgattca ttgtttcaaa tatttctttg gctttttgat tcatgaaaac
    21841 tttttcctga ctctcaatcg aaaaagcaac ttctctattt tttaataaac aaacaacttc
    21901 ctttatcaat ctttgattaa aagattgatt ataaagtaat tcaccatgat attgaatgta
    21961 attacctcct cctgcaatat atccatcaac tcctaatgat aaaacatcat cttgtattgt
    22021 acccatactt cttccagtac aaataacaac actgcaatga ttcttttgac ataaacgtat
    22081 agcatgcttt gcagattctg ggattccata tacctcatct ctaagcgtac catctatatc
    22141 aagtattaat aatttgtaca atttcatctt cctttcttac tatcaaaatc ataatagcat
    22201 tcaaaaaaaa agatatcaat attttgatat cttttaaaaa atatgttcat ttttagcaca
    22261 aattttcatt ttcggggaat atgttcactt tctaaataca tcttgtattt aatataaagc
    22321 agttcaatta aaataaaata aggtgttgtt acatcgtagt ccagttgttt atctacagaa
    22381 atagatatag attgaatata caggttttca tcacataatc ttgctaaagg attacttatc
    22441 atttttgtaa tagaaaccat ataaattcct ttcattctca tttctttagc aaaatcaata
    22501 attgtagatg catctccatt caatgaaata aagaaaatca tatcatctgt ttttataaaa
    22561 tgattaagtg cctgaatcat atcataacca taaacatcat atataatttt ccctgttggt
    22621 aagaaaatcc ttttcatctc tttagcaact ctttcctgag aataaccttc tccaaagacg
    22681 attatacgat ttgccttttt cattctttca aacataatcg aacaatcctt cttctctata
    22741 tcatttatca ccttattata gcaatccatt aattggtgtt tactttgatt tttatattct
    22801 acttctgttt catttaagcg tataataact cttaattcac tatatccagg taattgtagt
    22861 ttttgagcaa aacgagaaag tgcacttgta gatatatggt atttgtctgc aaattcatct
    22921 atagaaagtt taatacaatc ttctttatga ttaagaatac actcacagat atacttatca
    22981 tttgctgtaa atgaattata atatttattg aaaagtcctt caattttcat tcttttctcc
    23041 ttctatactc tatgtaattc atatgaagca aatctaatag agcataaaat gctcctacga
    23101 cttcataaga tatagcttgt aatgtatcaa gtttttgagt ttcaacatac agattatact
    23161 cacaaagtgt tgcaattgta ttattatcaa gcctagttaa agaaatcgta tgtatatgat
    23221 tgttgataaa ttttgcaatt tcaattcctg cttttgtttc tccagacaaa gaaatgataa
    23281 taaataaatc acttttttga aatgattctc tcataaattc aatttcacct atatcaaata
    23341 agtcaataac acatttacct actgaaagaa atattctctt tatttcttga gctaaacttt
    23401 tctgttcatt acctgtccca taaatataaa ttgtttctgc atcatatatt attttacaaa
    23461 tatcttgaaa tgatattttt tgtaattcat tcattaatag tcgataattc tcgcaaacat
    23521 catgaaaatt attatcatta ttaacaaatt ctagttgatt atttttaagt aaatacttta
    23581 aatcagagaa tgaatctaat ccaatttttc tacaaagcct taataaagta gctctagaaa
    23641 cattacattc atttgcaatt ttttcacacg aaaaattacg gtattctctt tgacaaatca
    23701 atatttgttt aataataatt ttatcatttt catttaaatt atcataattt tggttaacta
    23761 acgattctaa tctcattttt tccactccct aaatattatt ttaatataca ttagttgctt
    23821 tttctaggga agagttttaa ataataagga taaaataatc atttgatttt atttttatta
    23881 aaataaaaat aacctgctaa aagcaggcac caaaaagtac ttatcctttc gaacatgtac
    23941 tttagatatt gctttcttcg gaagcttcat ctcttcatac tctaaaatag agttttttct
    24001 aactcaaaag tattttccat ggtagaaatt actttaattc atctgccatt tattttttat
    24061 cttatttaat aattactact acttgaatat ttacttcttt aagtaaattt catagttaac
    24121 aaagctaaaa ttattattat agatgttaaa atacaaaatt gtaagtattg ttataaatat
    24181 gcttacagct acaaatatac atgatatatt aaaaagaagc tgtaaattat tatcatacat
    24241 acaagcaact acgcctctat taatccatgt taatttttat tatttaatac aaggagttcc
    24301 atgagacata catatccatt catattttaa attatccaat tctcttatag atttcatcat
    24361 ctttttataa tcccaattcc atggattagg ataaggaact atatttccat tcttattttt
    24421 gactaaatca cctgcaaata atacatcttc aaaaatcatg cagacatgcc ctggagtatg
    24481 accaggagta tggataattt ttatattccc aatctgcata ttctctccat aaggttcaac
    24541 atccttaatg agccttctgg aaattgcctt tccaataaac ttcttaaacc caggtctatc
    24601 cttctctcct ataataaaag gtatatcctc tacatgagcc catacttttg ctcctgtttt
    24661 ttctttcaat aatttaatat tagatatatg gtcaacatca tgatgagtta ataaaatatg
    24721 cttaatatct gtaagtttaa tgccaagtgt ttttaattct ttaattatat ttttccccat
    24781 aaaaggaaga cctgtgtcta taagaataat ttctttatca aacacaataa aacaatttga
    24841 gccacgagta ctatctaaca ttaatacatt ttcttttatc ttcataaata aattcccctt
    24901 at
//
LOCUS       DAELTA010000042        24815 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.042, whole genome shotgun sequence.
ACCESSION   DAELTA010000042 DAELTA010000000
VERSION     DAELTA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.042"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            587..1243
                     /locus_tag="KPG13_001505"
     CDS             587..1243
                     /locus_tag="KPG13_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985240.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1669..2940)
                     /gene="sleC"
                     /locus_tag="KPG13_001506"
     CDS             complement(1669..2940)
                     /gene="sleC"
                     /locus_tag="KPG13_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG0985241.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            3185..3901
                     /locus_tag="KPG13_001507"
     CDS             3185..3901
                     /locus_tag="KPG13_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG0985242.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3980..5290
                     /locus_tag="KPG13_001508"
     CDS             3980..5290
                     /locus_tag="KPG13_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG0985243.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5390..5926
                     /gene="lepB"
                     /locus_tag="KPG13_001509"
     CDS             5390..5926
                     /gene="lepB"
                     /locus_tag="KPG13_001509"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0985244.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            6092..6628
                     /gene="lepB"
                     /locus_tag="KPG13_001510"
     CDS             6092..6628
                     /gene="lepB"
                     /locus_tag="KPG13_001510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG0985245.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6638..7393
                     /locus_tag="KPG13_001511"
     CDS             6638..7393
                     /locus_tag="KPG13_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG0985246.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7467..9134
                     /locus_tag="KPG13_001512"
     CDS             7467..9134
                     /locus_tag="KPG13_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG0985247.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            10101..11030
                     /gene="glsA"
                     /locus_tag="KPG13_001513"
     CDS             10101..11030
                     /gene="glsA"
                     /locus_tag="KPG13_001513"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG0985248.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            11128..11691
                     /locus_tag="KPG13_001514"
     CDS             11128..11691
                     /locus_tag="KPG13_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985249.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            12179..13015
                     /locus_tag="KPG13_001515"
     CDS             12179..13015
                     /locus_tag="KPG13_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG0985250.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            13299..14294
                     /locus_tag="KPG13_001516"
     CDS             13299..14294
                     /locus_tag="KPG13_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0985251.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14551..14955)
                     /locus_tag="KPG13_001517"
     CDS             complement(14551..14955)
                     /locus_tag="KPG13_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG0985252.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15977..16891
                     /gene="queG"
                     /locus_tag="KPG13_001518"
     CDS             15977..16891
                     /gene="queG"
                     /locus_tag="KPG13_001518"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG0985253.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16997..19234
                     /locus_tag="KPG13_001519"
     CDS             16997..19234
                     /locus_tag="KPG13_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0985254.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            19432..20601
                     /gene="nth"
                     /locus_tag="KPG13_001520"
     CDS             19432..20601
                     /gene="nth"
                     /locus_tag="KPG13_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG0985255.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            20795..21265
                     /gene="trmL"
                     /locus_tag="KPG13_001521"
     CDS             20795..21265
                     /gene="trmL"
                     /locus_tag="KPG13_001521"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG0985256.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            21301..22149
                     /locus_tag="KPG13_001522"
     CDS             21301..22149
                     /locus_tag="KPG13_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0985257.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            22437..23441
                     /locus_tag="KPG13_001523"
     CDS             22437..23441
                     /locus_tag="KPG13_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0985258.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            23567..24679
                     /locus_tag="KPG13_001524"
     CDS             23567..24679
                     /locus_tag="KPG13_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0985259.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
BASE COUNT         9858 a         2327 c         4651 g         7979 t
ORIGIN      
        1 atagagtaat aatatgatta taaatttatt gattaatttt attattattt ttaagggggg
       61 agagtatttg tagttataaa gcatattggg aataatgaaa aaggttaaaa acatattact
      121 ataaagtata gggggtaggt taaaagagta atcataaaat aaatatacag ataatagtag
      181 ataagaatac cagaaaaaaa tagatagtta aagggttagg gtgaaagagt aatcgtaaaa
      241 taaatatatg gatgatggtg gataagaata tcagaaaaaa tagatagtta aagggttaga
      301 gtgaaagagt aatcgtaaaa taaatatatg gatgatgtgg ataagaatag taagaaaaaa
      361 tagatagtta aagggttagg gtgaaagagt aatcgtaaaa taaatatata gataatggtg
      421 gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat atggaaaatg
      481 aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt aaataaatct
      541 ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga aaaaaggcga
      601 ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac caagtagtag
      661 agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt taaagtttgc
      721 tgtattagct acaatgggag atttacttgg tgttagaatt ttgtataagg attggaaaat
      781 accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag ttaccacata
      841 tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta tgcttccatt
      901 tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata tactaatgtc
      961 accaactgta tttttattcc ataaggttat ggactgtttt atagataaaa aatatgaggt
     1021 tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact ggaatgctta
     1081 ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc atacaattgt
     1141 atttttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta tagctttagg
     1201 tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact gatgaagata
     1261 gtagtattat ttaaattagg aataactttt taaattaata ttatttgtat ttataataaa
     1321 taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa taataagtga
     1381 gattttaaat aaaaagtagg tttttcgtta gaatagcatt ttattaaact taaaaataaa
     1441 tataatattt taaagaaatc taagttgcta agcactgttt ttaagaagct taaaatcccc
     1501 catttaaagt tttaagattc taaaaaaaca agttataact aaaaaatggc tgttaagact
     1561 acaatactta taagtaatac ttatggtata tattagttca taacagccac ttttatttta
     1621 aattaataat tttattcaat aatttgagtt tatgttctaa atcaagcttt aaattaaagg
     1681 atttaaagaa gctattctag ttgtagctac ataaactctg gatatttcat accatgtttt
     1741 aaaatctact actccgcttt gaggtaaacc aaagattcct tggaatatct taacagcgtt
     1801 ttctgtatct gttccataga taccatcttc tataactttt ggtacagctg gataagaatt
     1861 agatatagca tttaactgat tttgtatggt tctaacatac tgcccagaag aaccaacttg
     1921 tagtgtagta ccagggaatg acactggtac accacttact ataggtgctc tttcaaatac
     1981 tatctcatct ccatagaaat atcttagtat actctcatag tcatatcctt ggtcacctaa
     2041 gtctttactg ccccattgag tcatttggtc aggacattgg gatttttgtc catcacaata
     2101 ttgtgctaat agtggctgtc ttgatgtagg aggtcttttt ataaaagtat taaaaatagc
     2161 atctacaact acgttaattg gttcaaataa gtttctatta tttataaact tatggtcgta
     2221 tgctgttgtt gaagttattg taaagttata tcctttattt ctataccatt cagtaaagac
     2281 tctatttaaa gtaaatgata ttatagctat tacatttgca tagattgtct gttcaggcca
     2341 agttgaatat atttcagatg acgctatgtt tttaatgtac tctttaaatg gaatccaata
     2401 gtttggagct gatgaatctt ctggcaaacc atcatgaact actataaatt caggcactac
     2461 ggggttatct aatacaacga aaccagtagg aggtggtaat ggttttaagt tactttctgg
     2521 aatcttagga ggataagtac catatagagt atgttctcct atatcaaaaa tcaactcact
     2581 ttgtcttgag aaagaacgct tacttcttgt tctaggagac atgggaactc cttgtcttgc
     2641 ttcaattgta gctaacaact gtgttccttc tatgacaact gtctcatatc catctgctat
     2701 ggcttccact atatactggc tataaggtct aacatcagat ggttgtagag agtaatctac
     2761 atctggtgct ggtaatacta aaccagtcac ttgaccagat tcattacttc ttaggttttg
     2821 gtataaagtt gatgagcttt ggctaccatt agacattgaa tatatgttta ctactgcatt
     2881 ttgaataggt ctattgttag tagcatcaat tatgcttact gttaagaaac catcttgcat
     2941 caaatcaccc tttctttaaa tgaatttagt tcccttatat ttcaagatat gaatatagct
     3001 tgaaatttgt taaataatat agatataaat ttaaattttt ttgagtattt gagtatattt
     3061 gtatttaaat aatttaaaat aatttaatgt atattttagt atgtatttaa aacacgaata
     3121 agttaaaaat aagtagatga tataaatcat aaataaatca aaaattttga aaggaaaata
     3181 atctatgaag ctagatttat ttttattagc aataattcct atattgatag ggatgttttg
     3241 gataaggtct aaagatagat attgtagaga acccctaatc catctaatta aatttttttt
     3301 aataggtgct tttctaagtg ttataattat acttttagaa aatttactga tgaagtttaa
     3361 tgtatttgaa gggtattcag aactaatata tgtatcattt gtagttgcag gacttgtaga
     3421 agaaggtgta aaagctctga tactgattcc tgccctaatt aaagaaaaac actttactga
     3481 aaagttagat ggtattattt actctgtatt tttagcttta ggatttgcaa caattgaaaa
     3541 tatggtttat attttttctg aaagtagaaa tctagcactg caagttggta ttaatagggc
     3601 agttatctct ataccagctc atgtaatgtt tgcaattact atgggctact atatttctaa
     3661 gtataaattt gagggaaata aaaataaaag acgtgagtat ttgtttatgg cggtgttaat
     3721 tccaatttta ttacatggag tttttgattt catattgatg atagaatata gatgggctat
     3781 tatattactt atagtttatg taataatttt gtggaagata aatcttgata aacttgagaa
     3841 atatatgaat cactctaaaa aagtgttttt cggtaatctt aggaaaaaga agaagaaata
     3901 aaatttaaag gaaagacata aataattaaa gtataataaa tgacacacaa gtaaagcaga
     3961 ttgaaggggg attatagatt tgacagaatt accaaaggaa tttttagaag atattgaaga
     4021 aatactaaaa gatgaatatg atgattttat aaaaagttat gaggaaagta agactactgg
     4081 gcttagaata aatactttaa aaattgacaa gaaaaaatta tttgatttgg agttgtttga
     4141 tttgaatcaa ataccatggg cagaagaagg tttttattat gatgataaaa ttgacagacc
     4201 tggtagaaat ccactgcatg aggctggagc atactatttg caagagcctt ctgcaatgag
     4261 tgtaatacca aaggcagata ttaaagaggg tgaaaaagtt ctcgatatgt gtgctgctcc
     4321 tggtggaaaa tctacatata tactatctaa gctaaacgat acaggtttat tggtttctaa
     4381 tgaaattaat gctagtagaa ttaaggctct aggggaaaat ttagagcgat ttggagcgag
     4441 aaattgtatt ataacaaaca ccgattctaa tagcttaaga aagacgttta atgggtattt
     4501 tgataaggtt attatagatg caccttgttc tgggcaaggt atgtttagga aggatgaggt
     4561 tgccatatca gattggagtt atgcaaaggt agtagaatgt caatccatcc aaaaagagat
     4621 tataagagat ggatataaaa tgttaaagaa ggatggcatt ttaatttact caacttgtac
     4681 tttttcaagg gaggaaaatg agcatgttat aaatgagttt atcagtgaat atccacatgc
     4741 agaacttatt gaaatggaaa gattatggcc tcataagata aagggagaag gtcattttgt
     4801 tgccaaaata aaaaaattag aagaagaaga atgtaatgtc aaagaattaa aaataaaaaa
     4861 tttagataaa gaaataaagg attataggga gtttgaaaag aaatttttaa aggttgattt
     4921 ttatagtaat aaaaaaaata aattttattt aagaggagaa aatttgtacc tacttcctga
     4981 aatatatcca gatacgaaaa aactaaaagt attgagatat ggtttgcatt taggggtttt
     5041 aaagaagaat agatttgagc cttctcatgc actatctcat tacttaaaag ttgaagatgt
     5101 aaaaaatgtt gaaaacttca gtgtacagag tgaatcaatt cttaaatatt taaaagggga
     5161 tgttgtaaat tctaatgata gtagaggatg ggttttggtt tcggttgaag gaataccttt
     5221 aggttgggga aaagaatctt ctggtgtaat taagaatcat tatcctaaag gtcttagaaa
     5281 agtattttaa actattagaa aaaagaagta taatatttat attgtaggtt tatggtagat
     5341 tattttgctg tagattgact taatatttaa cacagaggag acgattttag tgggtgaagc
     5401 agttaaaaaa gaagttgtag aatggataaa agtgattgtc atagctcttg ttttggcatt
     5461 tgcaataact cgttttatag tgccaacaat agtcaaagga gaatcaatgt atcctacatt
     5521 agttgaacgt gattatttga tagttaacag aattgcgtac aaggtaggag agccaaaata
     5581 caaagatata atagtattca aaaccgactt aacagaggaa aatggaaaga aaaaagattt
     5641 agtaaaaaga gttatcgggg ttcctggtga ccatgtaaaa atacaagact ccaaggtata
     5701 tgtaaatgat aagttgttag atgagacttc ctatatacat aataatcgta ctgatggaga
     5761 tattgatatc gtagttccag aaggaaaatt atttgcaatg ggagataata gagaaaaaag
     5821 tttagatagt agatacgatg aggttggatt ggtcgacgag cataccattt taggaaaggt
     5881 tctagtcaga ttgtatccat tttctaagat aggaactatt gactaaacat ataaaatttg
     5941 gagcaatcgc tgattagctg aaatcaccag accatacaca atagttgtct atataaaaaa
     6001 gacaacacct tatttagtag acttatctaa aagatagtgt ttaagagaca ttatcaataa
     6061 agataatata actataaagg agaatttgtg aatgaatgaa actattaaag aagagattgt
     6121 agagtggata aaaataatta ttactgcact tttttttgca tttattataa ctcgttttat
     6181 aaaaccaaca ttagtaaatg gagaatcaat gtacccaaca cttaaatcac atgattattt
     6241 ggtagcaaac aggatgacat ataagttatc agaaccaaaa tgtggagata taatgatatt
     6301 taagactgat ttattacaag agaatggaag gaaaaaagag cttgtaaaaa gggttatagg
     6361 tgttcctggt gaccatctaa aaattaagga ttctaaggtt tatataaatg gtaagttatt
     6421 aaatgaagtt tcatatatac atgataatta tactgaaggc gatattgata tggtgattcc
     6481 taagggaaaa gtatttgcga tgggagacaa tagagaagtt agtttagaca gtagatataa
     6541 agaagtggga ttagtagatg aagaaaatat taaaggaaaa gttattttaa gagtatttcc
     6601 ttttacagat ataggtattt ttgagtaggt gatttgtatg gcagttggta tagcgtcctt
     6661 attgtttaat attgaggagg ctttaaacat ttgtgagagt ataaagcaaa taactcattt
     6721 agagattgga attgacaata tatctgaatg tagtgagtta tgtaaatata aagagagaat
     6781 ttctaagtta ggattatcta tagggataca tttgcctatg gaattaaata cttgcgaaaa
     6841 catagagtat atacgaaatt catggataaa ttttattgag aagatagaat ttgaactaaa
     6901 aggatttgat ttaaggtatt ttaaccttca tttgggatat gttatgacaa atagagttag
     6961 aaagaataga gacaaatatt taggaaatag tgtggatttt ttagataaat taaatacaaa
     7021 ttcatatgta tgtatagaga atacttattc aaaaggtggg gatttttcaa atattggaaa
     7081 tatatcttat gattttgaat atatatttaa gaggattaaa aattctaaga tttgtttttg
     7141 ttacgatact ggacactgtt taattgatga ggatgcttat gtaaagaact taaaggataa
     7201 aataagactg atacacttaa gtgataatga tggtatcaat gatacacatg ttggtatagg
     7261 aagaggaatt ttatctgaag aggggattaa agagatctta acattagatg cagaatattt
     7321 agtattagaa ataaattacg aagacattga agatactata agtaaattga ataatattgt
     7381 aggagagggt taaatacctc tctttatctt gttgatatgc ctcattaatt aaaatttata
     7441 ttcatcaaaa ggaaggagat gttgaagtga aaaaaataaa cttaaaaata gaaggaaaag
     7501 ataaggaata ttcattagaa gaaaattccc ctggaatcag attgggagat atagcaaaag
     7561 aattttgtga tgagcataag ggttatataa cacttgcagt tgtagacaat aaactaaaag
     7621 agctaaattg tagagttaag aaggattgtg agataaattt tttagatact actaatgaag
     7681 atggagagag agtttacttt agagtcatgt catttatatt tgtaatggca tgtagagaaa
     7741 tcttttggga tagcagagtt acaatagaac attcactatc agatgggcta tattgtgaag
     7801 ttcatataga tagaaagctt aaagaagcag atgttgaaaa tataaaaaac aagatgaaag
     7861 agatagtaaa caacgattat gttatagaaa aaattgaagt tacaagaaat gaagctattt
     7921 ctatctttga aaataatgaa atgtatgaaa aggcagagct tttgaaatac aaagagtatg
     7981 atagtgctaa agtatataag tgtagaaact atatagacca tttttatggt tatatgttgc
     8041 catcaactgg ttatataaaa tcatttgata ttaagcttta taatggaggc gttataattt
     8101 taggacctag tgaagaagat aagactcttc ctatgaaatt tgtacctcaa cctaagctat
     8161 caagtgtata ttatgaagca gaagaatggt caagacttat gggaattgac aaggtagtat
     8221 ctcttaataa aataatagaa aacaatgaat atggagatat aataaggaca tttgaagcat
     8281 tgcatgaaaa gaagttatcc caaatagcgg atatgataaa ggatggcaat aagagagtag
     8341 ttttaatagc agctccatct tcatctggaa aaacaagttt tgctcacaga ttatcaatac
     8401 atcttagagt aaataattta aatcctattt caatttcttt agatgattat tttatagata
     8461 gaaaacatac tcctttagat gaatatggaa attatgattt tgaatcaatt tatgcaatag
     8521 atttagagaa gtttaactta gacttaaaga aacttttagc tggtgaagaa attgatgata
     8581 taagattcaa ttttaaagaa ggtaagagag aatacactgg taaaaagata aagatagatt
     8641 caaatcagcc aattatatta gaaggtatac atgggctaaa tcctatttta acatcatcta
     8701 taccagatga agataaattt aagatataca taagtgccct tactcaaata aatttggatg
     8761 accataatcg aatccctact gctgatttga gaatgataag aagaattgtt agagactata
     8821 attttagagg atatagtgct gataatacta ttttacaatg ggcatcagtt agaagaggag
     8881 aaaagaagaa tatattcccg taccaagagg aggcagatgc tatatttaac tctgcttgtg
     8941 tatatgaatt ggcagtttta aaacgatttg cgaaaccttt gttggaagag ataaaggaag
     9001 ataatcctgc acatattgaa gctactagat tactaaagtt tttgcagtat tttgttgaat
     9061 tggacgatac ttctgacata ccaggtattt caattcttag ggaatttatt ggaggaagta
     9121 aaatagttga ttaatgtgtg tattatagat tgttttatga agattaaatg gcatagcaag
     9181 tagtgttatt ggtttatgtt aagtatatta agtgtaaagt ggagaattaa attgatacag
     9241 attttaaagt ttgagttgat atataaaaag gcttgtttta tacttttgta tgaagcaagc
     9301 tttttatttt aaattttact tttttgttta atattttgtt aatttatgtg attatttttt
     9361 aaaagagtaa aagagtaaaa gagtaaaaga gtaaaagagt aaaagagtaa aagagtaaaa
     9421 gagtaaaaga gtaaaaggtg ttgggttaga aatttttggt gttataaaaa tttaaattac
     9481 tggattatta gattgctgga ttgctaaatt actaggttgt taaattattg aattattagg
     9541 ttgttgaatt actaaattac taggttgtta agttactgga ttatttagat agttagatta
     9601 ctaggttgtt aaattattga attattaggt tgttgaatta ctaaattact aggttgttaa
     9661 gttactggat tatttagata gttagattac taggttgtta aattattgaa ttattagatt
     9721 gttgaattac taaattacta ggttgttaag ttactggatt attagatagt tagattacta
     9781 ggttgttaaa ttgttaaatt attgaattgt tagattgttg aattactaaa ttactaggtt
     9841 gttaagttac tggattatta gatagttaga ttattagatt gctaaattat taggttgtta
     9901 aattactgga ttattagatt actaaattgc taggttgtta aattattaaa ttattaagct
     9961 atttaaacta tttaaattgt ttgaaaaatg aattgattct tgtagaaaat aattttaaag
    10021 tataattttt tttaacattg ttttattata gtataaactt gtgctaaaat agaatatata
    10081 acataatata agaggtgcat atgaatacgt tagatgagac attattaaaa gaaattataa
    10141 gttctaataa gaagtatact aattatggac aagtcgctag ttacatacca gaattaaaaa
    10201 acgctagaag aaatgattta ggaatttgta tcatagatag cgaaaataat ctttatagtg
    10261 ctggaaactg tagcacaaag tttactatac aaagtatttc aaaaccaatt gtgcttgcta
    10321 tggcacttat ggataatgat tgggaagacg ttttttcaaa cgttggaatg gagccaagtg
    10381 gggacccatt taattcaata atgaaacttg aaattaatga cacaaaaaaa ccctgtaatc
    10441 caatgataaa tgcaggggct atagtaacca cttcccttat aaatgggagt tgtttagaag
    10501 aaaaagaaga aagaatgctt agtttcttta gaaagttagc aaaaaatgat aatataggaa
    10561 taaactatga tgtctataag tctgaaaaaa tgactggaga tagaaacaga gctatggctt
    10621 acttattgaa aagtgatgga tttatcagag ggaatgtaga agatgttcta gacctttatt
    10681 ttaaacaatg ctcaattgag atagattctg tagatttagc gagaattgga ataaatcttg
    10741 caaattatgg agtagatata gaaaatggtg agcatttaat gagtgaaatg gtttctagga
    10801 ttgtcaagac atttatgatg acttgtggaa tgtatgatgc ttctggggaa tttgctatta
    10861 aagttggcat tcctgcaaaa tctggagttg ggggtggtat aatggcttct gtgccgggac
    10921 gcatggggat tggtgtttat ggacctgcgc ttgacaaaaa aggtaacagc gtcgcaggag
    10981 ttaaagtttt agaggagttg tcaaataaac tcaaattaaa tattttttaa aatattaaga
    11041 aaggattttt ttgacaacaa catataattt aatatataat taaattgtaa agaaatatga
    11101 attaaataaa ttttttagga ggggtcaatg aaagaggtat tggtattacg agatttggag
    11161 tgcataaagg ctattgcgca tccacgtaga atagatattc taaaagcatt tgacaaatcg
    11221 ccattgtcag caaaacaatt atctcaatta ttggaagaac ctcatgcaaa aataaactac
    11281 catataaaaa tgttatacaa agttggtatt ttagaattag ttgaggaaaa aataaagtcg
    11341 gggattgtgg aaaagtacta ttatccaagt gcaaaaaatg tggttatagg gaataggata
    11401 cttaattttt ctttagacaa tggggaagaa aaagaagaat tgtacatatc aaaatttgaa
    11461 aatatgagtg aagtttttta taaggcagca gaggaagatg ttttagaaaa tgaaaatata
    11521 gttgattatc atgatatttc tcttactcat gatgaattag ttgaacttag tgacactatg
    11581 aagtcaaaga tagatgaaat tttaaataaa agacaacaca atgttgaagg ttctaagtat
    11641 gatatagcta tggttatagt tcctactctt gaggaggaat gtcctagtta gagaattaat
    11701 tatggattaa ttaaagatat aatattagag attgaacatc aaaatttact taataatcaa
    11761 aaaatagttt ttggtatggg taaaacaatg atttttaatc tctatttttt tgttttttta
    11821 tacctattgt atttgtaaat aatatgtata gtgtaagatg tatacactat ctaagaagtg
    11881 cttagataat atacaaagta tgtaagatta taatatttga aattgattaa aatgggtata
    11941 attaaagtgt aaatattaaa attaaaaatt aatataaatt aaatcaatgc tagaaatatg
    12001 ttaaaagagg aatgagatta gttgggatag aacatccctt agagcttacg aatttttagt
    12061 gaaaaagtac tatcaaatag gaatctccat cttcatatgt tggctaaatg tagttaaggt
    12121 ggagtagttc aaaattaaaa ttgagtacaa gtagtagtac aaataaaagg agattaatat
    12181 gattaaatta ggaacacatc tttcaatagc aaaaggattt gctaatgcgg ctgagacagc
    12241 tgtatatatt ggtgcaaata catttcaatt ttttagtaga aatccaagag gaggaaatgc
    12301 taaagaattt gatgaaaaag atataattaa atttcaagaa attagaaaag aaaataattt
    12361 tgcaccactg cttgcacatg caccatatac tatgaatctg ggtggtacaa aggatgatgt
    12421 atacgaattt gcaacaaggg tgatagatga ggatataaga aggatggact cattaggaat
    12481 tgaatacatg tgttttcatc ctggaagtca tgtaggtggt ggtgtagatt ttggcataga
    12541 tagaatagta aaaggattga acaatgccat aaagggtgat gaaaatataa ctatacttct
    12601 tgaaactatg tcgggaaagg gaactgaaat aggttttaaa tttgagcaat taaaaagtat
    12661 aatagacaaa attgagcata aagaaaaaat aggtgtgtgt cttgatacat gtcatatttt
    12721 ttcagctggt tatgatatag tgaatgattt agatggggtt ttggaagaat ttgataaggt
    12781 tataggcata gaaagattga aaactgtcca tctaaatgat agtatgatgc catttggaga
    12841 caaaaaagac agacatgctc ctatagggga aggaaagata ggattaaaag cattaattga
    12901 ttttatggaa catcctagtc taaaacattt accatttttc ttagaaactc cttttgatga
    12961 tgaaggtcac aagagagagc ttaaaatgat aaaagaaata ctatctagta aataaacctt
    13021 tattaaaaat atgtgaaata ctccaccctc aacataatta gggtggagat acaatcatga
    13081 aatgtgataa tttaaattaa tggataggaa taacgaaaat tgtatacaaa ttaactgggt
    13141 ataaagtatg attatattat gaatagactg atataaaata taattatatt atgaatagta
    13201 tgtaaataca ttgcaaagat tctcataatt tgttgtataa ttaaaggtga catatttata
    13261 ttatataaaa aattattaaa agttaggtgg ggaaagatat gagaaattta ggcaatacta
    13321 acatgaaaat aaagagagtt ggctttgggg gcattccaat acaaaggatt actcaagatg
    13381 atactaactt agttataaat gagttagaaa aacaaggaat aaactttatt gacagtgcaa
    13441 gagggtatac aataagtgag gaagcaatcg gaattgccat tgaaggaaaa agagataaat
    13501 ttttcttagc tactaaaagt atgtccagag attatgattc aatgaagaga gatgttgaaa
    13561 taagtttaaa taattttaaa actgatttta tagatttata tcaattccat aatgttaaag
    13621 aagaggagta tgataatctt tttaaagata aaatggctta tagtgccctt ttagaggcaa
    13681 aggagcaagg taagataaaa catattggta taactagtca caatttaaat actatagaaa
    13741 aagctataga agatggaaaa tttgacacta tacaattccc ttataatata gttgaaggac
    13801 aagcagatga agtgtttaaa aaagctcatg aaaaaggaat tgggataata gtaatgaaac
    13861 ctttagctgg aggtgcttta gacaatgcaa cactggctat taaatatata ctatctaaag
    13921 agtacataga tgtagttata ccaggtatgg agagtgttga acaggttaga caaaatgtcg
    13981 ctgtacttga aaatcttgtg ctagatgaaa aagataataa ggaaattgaa gaaataagaa
    14041 attctttagg taaaaaattc tgtagaagat gcgaatattg tatgccttgt gctgtaggaa
    14101 taaatatacc attgagtttt ttatgtgaag gttattatac tagatatgga ttaaaagagt
    14161 gggctaaaga aaaatatgag gttatggatg ttaaacctac tgagtgtatt gactgtgggc
    14221 tttgcgagag tagatgtcca tatgaacttc ctataagaga aatgcttaag actgttgttg
    14281 aaaagctagg atagatttta tttattgaaa tcggtgtatt aaatttactc cgatttcttt
    14341 ttattagatg tggtcttata tacaaatatt aaatttacat agttatagct attatataaa
    14401 tttaatattt gtttaatact tattaaatat ttacttaatg ctttttaaaa acatttgttt
    14461 gagtatagac ataacacaat tattattgtt gtattagtat agtttcaaat actggagtat
    14521 caatataatg tcgaataaca cataaaatct ttatttttca aaaatattat aggatatatc
    14581 gtatttagat actggataaa gttcaatttc aaccctaggg ttatctttat ctataaaatc
    14641 aaataaaagt actggcttaa gttgtttatc attttttatt attccacttt tttcaatacc
    14701 atcacaaata ctttttggat aattatgtaa atcccccatt ctcttatttg ggaagaataa
    14761 tttcaagact gttattagat tttcttcgac agtttcccct tgatactgtt ggtttataaa
    14821 acctgcaatc atgttttcgt atgcaagata cttagaatat tttccctttg aaggcatcca
    14881 tgcttgacca tttatgttat gaagcttaaa attagattta ctaataggtc tacctggaat
    14941 tgttatttta atcaaatttt tcaccatcct tatacagtat ttattataat ctgtatatga
    15001 tttaaaagca aattgttatt taaagtgttt ttacagagct tttttgtaaa attttccact
    15061 ttagatgtag taatataaga taagtatttt gcttacaaat tttaaaattt gatatatgat
    15121 ggacatattc atattagtct attataagac cttcataata ttaataagta tagtttagta
    15181 aagagagtaa aaagaaatcc atataagtca agtaaagatt taataaatgg caatgtaaat
    15241 gggatattaa ttataagaaa aaatcaatct ttttgcttaa ctatagaaag aatgtcaaaa
    15301 tataactaat gatatttgaa gttttagagt ttatattaaa aggtaagaag gttaagtggg
    15361 taaaaaaggt tgaatagatt aaaaaggcta agtatctaaa agagttaagt aggtaaaaaa
    15421 gttaaataag taaatgaaaa aggctaagta tctaaaagag ttagtagtta aataagtaaa
    15481 tgaaaaaggc taagtatata aaggagttag tagataaaaa aattaaatag ataaaaagat
    15541 taagtatcta aaagcgttag tagataaaaa aattaaataa ataaaaaaga ctaagtattc
    15601 aaaagagtta gtaggtaaaa taattaaata gataaaaaga ctaattattt aaaggagtta
    15661 agtagataaa aaagttaaat aagtaaatga aaaagacgaa gtatctaaaa gagttagtaa
    15721 ataaaaaaga tgaagtatct aaaagagtta gtaggtaaaa aactaaatag ataaaaagat
    15781 taagtatcta aaagcgttag taggtaaaaa attaaatagg taaaaagacg aagtatctaa
    15841 aagatttaag taggtaagaa aattgaatag ctaaaaagat taagtattta aaagagttag
    15901 tagctagaaa aggttaaaaa gactaaaaaa attaaataaa tttaaaaagt caattagatt
    15961 aaaaaggaat aattttatgg ataataagga attaaaagca ttttgtaatt taataggatt
    16021 aaactgtgta ggagtagcag gggtagataa gtatgataat ttagaaaaga tacttaagga
    16081 cagacaacaa aaaggttatt taactggtat ggaagaacca ataattgaga acagaattaa
    16141 cccaagacat ataatgaagg atgctaactc tataatagtc tgtgcatttc catatcatat
    16201 aaatagacaa gaaaagaata aaaattcaaa tttatcaaaa tattgtcatg gaaaggatta
    16261 tcacattgta gtaaaggact ttttacaaca aatttgtgat tatatttcta aaacagtaac
    16321 agattttaaa tacaagttat ttgctgataa tggaccatta gttgacaggt atttagctta
    16381 tttatcagga ataggatatt ttggaataaa taataatatc ataactgatg aatatggttc
    16441 ttacgtattt atagggtata tagtaaataa ttataaattt gaagaagata aaccactaga
    16501 aaaaagttgt tttaaatgtg gaaagtgtgt aaaatattgt ccgggaaatg ctatattggg
    16561 aaattatgat atgaacccca aaaaatgttt atcatatata actcaaaaaa aaggagattt
    16621 gtcagagaaa gaaaaagttg ttttaaagaa tggcaaaaag gtatttggat gcgatatttg
    16681 tcaagaagta tgtccacata atgttgaaat accaacaact catattttgg aatttaaaga
    16741 aaatataata gattatttga attatgatga gatacaaaat atttcaaata aagaatttaa
    16801 aaggaaatat ggagatagag cttttagctg gagaggaaga aatataataa agagaaatat
    16861 ggaaataatt ttagataagt atgatgacta ataataagag tctgtttcat atactttaat
    16921 gtgtatttac tttaacactt gttgtgtaaa agtcatatta tatcattaaa gttaaatatg
    16981 gggaggtggg ggaatattga aatataataa tacatctgaa ataaatccac tagattatat
    17041 attgggtcaa gaaagagctg tagaagctat ggaattaggg ttgaaaataa ataatccagc
    17101 gtacaacata tatatagctg gtgaacctgg aactggtaaa agtacttatg ccttaaaggt
    17161 tttaaatgag tatgcatcca aaaaaaatac gcataaagat tggtgttata tatataattt
    17221 tgaaaaccct agagagccta ttatagttga attagaaaag gggtttggaa gagaacttaa
    17281 gaaagatatg gagaaattaa ttgaaagtct tttagaagat tttaaaaatg cgtttgaaag
    17341 tgaaaatttt gaaatagaaa aaaataaact tctggatgaa tatgaaatag aaaaagatat
    17401 gcttttgaag caaattaaaa aatatggaga agaaaaagga tttaaattaa aacagagtaa
    17461 aatgggaata gtttttatcc ctttgaaaga agaggaagat gaaagtgatg agtctgatga
    17521 agagttttac aaagctaaaa gagaactaga aaatatggcg atacaggtcg tatataaaat
    17581 tagaaactta gaagaaatag ctgaaaaagc agtgttagag cttgaagaag agatagctaa
    17641 acttgtagta gaacctcata tcaagagatt gtgtgaaaaa tatgaaaatt atgataagat
    17701 tcaaacttat ctggaaaata taaagaaaga tataatagaa tatatgtatt tattctattt
    17761 agatgaagag gagttaaaag ataagtatga taaagagcat tttattaaat ataaaattaa
    17821 cctatttgta gacaatggaa gctcaaagaa taaagaatca gcacctgttg tagtagagat
    17881 aaacccaagt ccagcaaatc tatttggaaa ggcagagtat gattatgcaa atggaaatat
    17941 aaagactgat ttcacaaaac tattatcagg agcatttcat agggctaatg gtggttattt
    18001 agtactttat gcagaccaat tactaaaata tacaatgtct tgggaaattc ttaaaaagac
    18061 tttgcagact aaaaaagtaa tattggaaac acaaacagca attaaaccag aaaatatgcc
    18121 attggatgtt aaattagtac tgattggaag ccactatata tatgatattt tatatagata
    18181 tgatgaagat tttgaaaaat attttaaagt atttgtagac tttgatagtg aaatggataa
    18241 aaatgaagat aatgaagaag gaatagcaag atttatagca taccaatgtg ataagaataa
    18301 tttaaggcat tttactaaat cagcagttga agaggttatt aaatttagca ctagattatc
    18361 tggtgattta gaaaaattat caactaagtt taataaaata atggaagttg taatagaagg
    18421 aagtgcttat gcagagttta gaaattcgga gtatacagag cagtgtgatg ttaaaaaagc
    18481 aataagtgaa aagagaaaaa gaatcaatag agtggaaaca catatggatg aatcaattga
    18541 aaatggattt actctaatag aaacagaggg aagaaaagta ggagtgataa atggtttgtc
    18601 agtcttaagt acaggagaat actcttttgg tagagcttca aggattacag cgaccactag
    18661 cccaggaagt aaaggcatag taaatataga aagagaagta aatatgagtg gctctataca
    18721 taacaaagga gtacttatat taggtggcta tctttctgag aattttgcac aggatttact
    18781 attgtcacta aatgcatatg tttgttttga acaaaattat ggtggaatag atggagatag
    18841 tgcatcttct gcagaattat atgtactatt atcatctctt agtggagtat caattaaaca
    18901 aaatattgct gttacaggtt ctataaatca aaagggtgat atacaagtag ttggtggtat
    18961 aagtgaaaag atagaaggat tttattctat atgtaaaact aaagggttaa atggtgagca
    19021 aggcgtcata attcctagaa aaaatcaaag aaatctcgtt ttatcagatg aagttaatga
    19081 tgcagtgcga gatggaaaat ttaagatata tactgttgaa agagtagaag aggctataga
    19141 gatattgaca gatgttaaat ttgaagaaat aaagaaattg gtaaaagaaa aattaatatc
    19201 tttcagcaag atacaaactg tgtcaaaaga ataagtttat tatagaattt aatgggtatt
    19261 tatgtataat aaaaagcaag aactttaata ggtttttgct ttttttgtaa atatatataa
    19321 attaaattct tattttgtat gttttatata ttgatgttta tttttttgtt gatatgatta
    19381 aactatttat aaatagcgtt ttataaattg gtattttgaa gggggataat tatgaaaaat
    19441 gtaaatctaa aagagataaa tgaaaaagat gtaattttgg aagaagagat aaataaaaaa
    19501 ggaatggata agaaaaatat aattgagaag gaagagataa ataaaaaagg aatagataag
    19561 aaagatataa ttgagaagga agagataaat aaaaaaggaa tagataagaa agatataatt
    19621 gagaaggaag agataaataa aaaaggaatg aataagaaag atataattga gaaggaagag
    19681 ataaataaaa aaggaatgaa taagaaagat ataattgaga aggaagagat aaatgaaaaa
    19741 ggaatagata aaatgattgg taaaaaagat gagataattg atgagaaaga tatagagaat
    19801 tttatggata ttgaagaaga taaaaaaagt atcaaagtaa gtaaaaaaag tactaaaaaa
    19861 ggtgaaacgg agaaaaaaac aaagaagagt agttctaata aggctacaca aaaaacaagt
    19921 aaagaaacta aaagtaagtc taaaattaaa aatgagtcag caaatgaaaa agacgtaaat
    19981 aagattttag acgagttaga aaaattgtat ccagatgcta aatgtgagtt aaattatggg
    20041 acagcctttg aacttttaat agcaactatt ttatcagcac aatgtactga tgtaagagtg
    20101 aataaagtta cttcagagtt gtttaaaaag tacaacacag ctagagactt tgctaatttg
    20161 agcattgaag aaattagtaa agagataaag agttgtggtc tttacaaaag caagtctcaa
    20221 aaaataaagg atacatctga acagctatgt gaattatatg atggtgaagt acctgatagc
    20281 ttagaaaagc ttataaaatt acctggtgtt ggaagaaaaa cggcaggggt tgtacttagt
    20341 aacgctttta atcatccagc aatagctgta gatactcatg tatttagagt tagcaataga
    20401 ataggaatag tggacgagcc aaatcctcaa aaaacagaat ttgcacttat ggaagctata
    20461 ccaaaagaaa gatggtcaca ctcacatcat gtattaattt ttcatggaag aagaatgtgt
    20521 aaagctagaa atccagaatg tgcaagttgc ccaataaaag aagattgtaa ttattataaa
    20581 gaacttaatg aaacgaaata acttttagaa agtaagttgt acaagaggga ttttctgata
    20641 taataatact tgtgaaaatg ttagataaaa aactattata tacatgaaat aaaaattatt
    20701 attaacatta ttttatataa tagtataata ttattgttat tctgtttata aaataaaatt
    20761 tgataaataa aagcgatact tagggaggat taaaatgtca ttaaatatag tattagtaga
    20821 accagaaata cctcaaaata caggaaatat aataagaacc tgtgcagcaa caggttcaac
    20881 tttacattta gtaaaaccac tgggtttttc attggatgat aaacacctaa aaagatgtgg
    20941 gcttgactat tgggatatag ctgatataca atattacgat agctttgaag aattacaaga
    21001 aaagtatcca aatagtaaat actttttttc tacaacaaag gctaaacagt cacattcaga
    21061 tgtgaaatat gaagaaaatt gttttatagt atttgggaaa gaaacaaagg gattaccaga
    21121 acctttgttg aaagcaaata tggatacttg tattagagtg cctatgttgg atgttgaaaa
    21181 agctaggtca ttaaatttat caaattcagt tgctattgta gtgtacgagg ctttaagaca
    21241 aataggatac ccaaatatga ggtagtttca acaagcctaa atgagaaagg agcatactta
    21301 atgaaaaatc taaagataat ctgtgacagt ttatcggacg tgccaaaaga tttgttagat
    21361 aaatatgata tagatatagt ttcattaaca gttatactgg aggaaagaga atacaaggat
    21421 agaattgata tatctggaga tgaattttat aaaaggctta gagaagaaaa tgtatatcca
    21481 aaaacttctc aagctactta tgcccaattt aaggaaattt ttgagaaata tacaaatgag
    21541 gatagagaga tactttatat agcagggtct gccacagcta caggtacgta tcaaagtgct
    21601 gtaatggcaa agaatgatgt agatggtaaa atatatacat atgactctaa cttgctttgt
    21661 atgggaactg gtttacttgt aataaaagca ggagaacttg caagtgaagg aaagtctgtt
    21721 gctgaaatat tgcctgtttt ggatgaatta aaagagaagg cttttgttat attttcagta
    21781 gatacattgg aatatttaca aaagggaggt agaatatcat ctactaaggc agcaatagga
    21841 agtatattaa atataaaacc gattcttgaa ataagagatg gattagtttc gcaaatatct
    21901 caagtaagag gaaagaagaa tgtaatttct aagatgatgg aattgataaa atcaaattgt
    21961 ggtgatgatt tatcagacca aattgtgtat ataggatata gtgatgattt taaggaaaaa
    22021 gaacaattat ctaaggttat gcaagatgaa ttaagaccta aagaaattag atttttccaa
    22081 attggtgtgt gtataggtgc acatgctggt cctggtgtta ctggtatcat atgcttaaaa
    22141 aataaataat atatttttgg atgacattat ttattttttt attaaattag gatagaatat
    22201 taattgttga gatgttaaat tggatttatt ttggatgaaa ttaagtgttg atatattaga
    22261 tttagtttgt agggaagtgt gtaaatagat aagaatctat tgcacacttt tttatagtaa
    22321 gatttattaa aaaaatttca aaatgtactt gtaaattgta tataaaaaaa ttataaatag
    22381 gatgtaaaag taaataggat ataaaaatat cgtaaatgta tgaggagtga aatttaatgc
    22441 gtttcaaaag aatattgata ttgatgttaa ctgtagcaat gatagctgga atgttggttg
    22501 ggtgtgctaa taacccaagt aataatccta gctctagcaa agataataaa agtgatataa
    22561 gcgataaaag taattcaaaa aaatctgtat caatgatact agatattgaa ggaacaaata
    22621 atgaagctat gaataattca gctttattag cattaaacaa tgctcaaaag aaacttaata
    22681 tagatacaaa caaagtggaa tcagatgatt cctcaacttt ttctaatagt atagatattt
    22741 tatgtaatga caattacgat ttaattatag ctgtaggagc tagatttgca aaaccattgg
    22801 agatggtagc caagaaatat ccaaagcaac aatttgcaat tatagactat gagtatgaca
    22861 agcaaccttc taatataact tcaatttctt atgaagataa taaatctgga tacctagctg
    22921 gattaattgc tggaaaaatg actgaaagcg ataaggttgg ttttatagga ggaacaaaga
    22981 gttctagtag agataaattt gaatcaggtt ttagagaggg tgttaaattt tctaatagta
    23041 gtataaagga tatttcagtt gagtatgcag atgtttttaa agatagcaaa tcagtagaat
    23101 ctatagctaa aaaaatgatg gataatggag tggatattat tttttctact actgaagatg
    23161 attcaaaagc tgtaatagat gcagtaaggg caaaaaataa aaaagttata gcaacgaata
    23221 aagaccaaca tgagttagct ccagaaaatg ttataagttc tattgtaaaa gattttgaga
    23281 atcctactta taatctgata caaagttttg taaaaggaaa ttataaaggc ggaaaggtta
    23341 tagaaaacac agttaagtct ggaagtactg gacttgccca aaatagttct caaaatattc
    23401 ctccagatgt gttagaatat gttaataaga acaataaata atctactata atatccaaga
    23461 atacttatga gtttagttat gaggtgaatt ggatgttata atagacatat atagaaagac
    23521 atatgcagag gtaggtcaga cctatctaaa tcagggagtg aattttgtga aaataaaaag
    23581 gatattgatg gcttgctttg tagtagtttt tacaattttt atattagcta tatgtaattt
    23641 tgattttggg tttgataaag taaataagaa taattctaat atagatagtg ctaaatcgaa
    23701 acagagtgag tctaaaagtt tgctaacttc agcagaaaag gataaatcaa atagtaaaag
    23761 taagaataaa gaagatgaca cagaagatga ttcatatgta agaaaaacta ttggagtatt
    23821 gttagatacc gaaaacttaa ataacgaatc atataataac aatgtactat cagccttaaa
    23881 aagggctgac gaagattttg atgttaggat aaaacttgaa aaaccaaaga cttctaaaag
    23941 ttttaaagag agtatagaaa aactgcataa gtatgaacca gattttataa tagcagttgg
    24001 agccagattt gcagagccaa ttaaagaagc tgctactaaa tatcccaaac agcaatttgc
    24061 aatagttgac tatacttata aagttcagcc agctaatgta atttctgtat catttcaaga
    24121 taatgttact ggatatttag caggacttat tgcaggtaaa atgactgata ctgggaaagt
    24181 cggttttata ggaggagttg agggttctag tagagataag tatgaatctg gatttagaaa
    24241 ggggcttaaa acttcaaata aagatgctga gttactagtt aagtattcaa atatatttga
    24301 aaaccctgaa tcagctagaa ctattgcaaa ggaaatgaga gattctgata gagatgttgt
    24361 attttcagct acagaagatg atagtaaaag agctattcag gtagcaagag aaaatggtgg
    24421 taaagttata gctattaata aagaccaata cgatatggct ccagataatg taataagttc
    24481 aattataaaa gatgtagaac aacctgtata tgaactgata aagaacgttg caaaagatgg
    24541 ttttaaggga agtaaagtaa tggaatttaa aataaaagat ggggaacttg gcttgacttc
    24601 aaagagcagt agaaatatac caccagatgt tttggaatac ataaaaaaag agtataacaa
    24661 agtgaaagat acatattaat tattgatgta gaagattata aaaatatatt gcttaagttt
    24721 agattagcaa taaaaagata tatttattaa atttgtgcta actgtacaaa atatattgat
    24781 taagtttaga ttagtgataa aaagatatat ttatt
//
LOCUS       DAELTA010000043        24608 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.043, whole genome shotgun sequence.
ACCESSION   DAELTA010000043 DAELTA010000000
VERSION     DAELTA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.043"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            102..1475
                     /locus_tag="KPG13_001525"
     CDS             102..1475
                     /locus_tag="KPG13_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985260.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1731..2798
                     /locus_tag="KPG13_001526"
     CDS             1731..2798
                     /locus_tag="KPG13_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG0985261.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2942..3250
                     /locus_tag="KPG13_001527"
     CDS             2942..3250
                     /locus_tag="KPG13_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG0985262.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3243..4673
                     /locus_tag="KPG13_001528"
     CDS             3243..4673
                     /locus_tag="KPG13_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG0985263.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4751..5125
                     /locus_tag="KPG13_001529"
     CDS             4751..5125
                     /locus_tag="KPG13_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG0985264.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5119..7323
                     /locus_tag="KPG13_001530"
     CDS             5119..7323
                     /locus_tag="KPG13_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0985265.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7323..8732
                     /locus_tag="KPG13_001531"
     CDS             7323..8732
                     /locus_tag="KPG13_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG0985266.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8717..9781
                     /locus_tag="KPG13_001532"
     CDS             8717..9781
                     /locus_tag="KPG13_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG0985267.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9811..11232
                     /gene="nhaC"
                     /locus_tag="KPG13_001533"
     CDS             9811..11232
                     /gene="nhaC"
                     /locus_tag="KPG13_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0985268.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11661..12125
                     /locus_tag="KPG13_001534"
     CDS             11661..12125
                     /locus_tag="KPG13_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0985269.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12359..12721
                     /locus_tag="KPG13_001535"
     CDS             12359..12721
                     /locus_tag="KPG13_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0985270.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13316..14176
                     /locus_tag="KPG13_001536"
     CDS             13316..14176
                     /locus_tag="KPG13_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985271.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            14434..16548
                     /locus_tag="KPG13_001537"
     CDS             14434..16548
                     /locus_tag="KPG13_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG0985272.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            17089..18042
                     /gene="blaCDD"
                     /locus_tag="KPG13_001538"
     CDS             17089..18042
                     /gene="blaCDD"
                     /locus_tag="KPG13_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0985273.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            18386..19069
                     /locus_tag="KPG13_001539"
     CDS             18386..19069
                     /locus_tag="KPG13_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985274.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            19066..21726
                     /locus_tag="KPG13_001540"
     CDS             19066..21726
                     /locus_tag="KPG13_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985275.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(21907..23088)
                     /locus_tag="KPG13_001541"
     CDS             complement(21907..23088)
                     /locus_tag="KPG13_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985276.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(23088..23750)
                     /locus_tag="KPG13_001542"
     CDS             complement(23088..23750)
                     /locus_tag="KPG13_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985277.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(23882..24520)
                     /locus_tag="KPG13_001543"
     CDS             complement(23882..24520)
                     /locus_tag="KPG13_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985278.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
BASE COUNT         9542 a         2847 c         4349 g         7870 t
ORIGIN      
        1 gataagataa atacaatagc aacattttat taattttttg ggtttttata tatttaggta
       61 ctggggtgat atagttgaac caagagatat tttttaaaat aatgaatgaa atattggaaa
      121 atattgatga aggggtacat tttgttaata aagacgggaa aactataatc tacaataatt
      181 ctgtacaaaa aatggaaaaa atgaacagac acgatatttt aaccaattca ttttttgata
      241 tagtcaacaa aatgaaaata aacaggagta cactattaga agttttggaa aaaagggaag
      301 ttatcaaaga caatattcaa aaatatctgg ataaaaatgg aagagagata acagcaatta
      361 atactacaat accaattgat attaaaggcg agtttgtagg ggcacttgag atatcaaaga
      421 atatgacaac gatagaaaat ttactaaaca gaaattataa aaaaaattta gtcaataaag
      481 ttagtattag acaaaaaaag ggttatgaat ttgatgatat tttaggggaa agtaatttga
      541 ttaaagaagc aatagctaaa tctaagaaag cttgcaagtc tgatgcttca gtatttatat
      601 acggtgaaac tggaacgggt aaagagttat ttagccagtc cattcactat ggaagtaata
      661 ggaaaaacta tccatttata gcacaaaatt gtgcagcttt gccggaatca ctattcgaag
      721 gtattctttt tggaactagc aagggaggat ttacaggagc aatagatagg ccaggcttat
      781 ttgaacaagc taacaaaggg acattgttat tggatgagat aaattccatg cctattatgt
      841 tacaggctaa actattaagg gttttacaag aaggttatgt aagaagggtt ggaggaacta
      901 aggatatacc tatagatgta agaattattg caacgacaaa tgaaagtcca aaggtaattt
      961 taaacgagca taaaatgcga aaagatttat attataggct taacataatt catataaaaa
     1021 ttcctcctct tagagataga aaagaggaca tacctctatt gtgcaaaaaa tttatttcaa
     1081 agtataataa aaagttaaat aaaaaagtag taggaatttc agatgaagct attaaaattt
     1141 taaaaaatta cgattggcaa ggtaacataa gagagctaga aaacataatt tattgtacta
     1201 tgagtatgat ggaagatgag gtacaaatag aaacaagtat gattaatcta aacgactatt
     1261 acaatcttaa agagaataaa gagagtaaca attattcttt aaacgatttg gaggacaaaa
     1321 cacttagtaa gattatcagt gagatagaag aaaagtatat atatgaatca ttagaaaagg
     1381 catcttacaa tataactaaa gctgcatcaa ttttaggtat gaataggcaa aatttacagt
     1441 ataaaataaa aaaatataat atcaaaatat tataaataag caagataaaa aaacaaaatc
     1501 aaagtaaaat aaaaaagcaa aatagaggag caaaattatt ataaaagagc aaaatatttt
     1561 gctctttttt ttatttctca taagaaatat tattttttat ttatgaaaat acataataaa
     1621 ataattatag tttcaacgtt tcaataaata tttactttaa ttttaaagtt ggcacgattt
     1681 atgctttaac taattattga gaatatataa atttttagga gggtgtatat atgagaaaag
     1741 taagagtagg aatatgggga tttggagcta tgggaatagg tatggctaat atgattctaa
     1801 agaaagaagg tatagaaata gtatctgttt gtagtagaag tacttcagga aaaagtatgt
     1861 ataatgtttt aggaattgaa agaggagaaa gaccagaagt tattatcaat aaaaattatg
     1921 aagaagtttt tagagaaaaa agtgtagatg ttgttttgtt agcaactgat tcatttacta
     1981 aaaaggcttt tgataaaata attttcttat taaatagaaa aataaatgtt atatcaacag
     2041 cagaacaaat ggcatatcca caagctgatg atgcagattt agctaaaaaa atggatgaag
     2101 ttgcaaagga aaatggagta agtatacttg gaacaggaat aaatccagga tttgtattag
     2161 atttactagt attggcgctt tcaggcactt gtgaagaagt tactagtata aaagctaaga
     2221 gagtaaatga tttatctcca tttggaaagt ctgttatgga ggaacaaggt gttggagtaa
     2281 caagggaaga atttataaaa ggtgtagaag ataagactat agcaggtcat gtaggttttg
     2341 tagaatcaat aaatatgata gctgatggat taggttggaa attagataag atagaacaaa
     2401 caaaagagcc aataatgact acagtagata gaaaatctaa gtacggagag gcattggctg
     2461 gaaatgttgc tggttgtaga caatgtggat atggatatgt aaatggagaa gttttaattg
     2521 aaatggagca tccacaacag ataataccag aagcagaagg tattaaaaca ggagactatg
     2581 tatcaataaa aggtatacca aacattgatt tacaaataaa tcctgaaata cctggaggag
     2641 taggtactta tgcaatgata gtaaactcta ttccattaat aataaatgct agaccaggtc
     2701 ttaaaactat gttggatata ccagtaccta gagcaataat gggagatata agaaatcaga
     2761 tagaagttga attggaagaa gaatcaaagg ctaattagta agataacagc taactaatta
     2821 gtaagatagc aactagctaa ttagtaagat aacagttagc taattggtaa aataatgttt
     2881 aatttaaaat aacagtaaat taattagtaa ggcaatagtc aattaataag gggatgaaaa
     2941 aatggatgct aaaattaatg attgggttat tatacacaat atagtattga ctccagaaga
     3001 aagagctcca caagttccag aggatactaa gaaagtatcc ttagaaatgt gggttaaagg
     3061 ttttatacaa agtgatgctt ctataggcga cttagtagaa gttaaaacaa ttacaggcag
     3121 acttgtaaaa ggtaatttac ttaaaataaa tccatattat actcatgatt atggtaaatg
     3181 tataccagag ttactccaaa taggaatcca agctaaagaa atattatttg gaggtgtata
     3241 taatgaataa ttcaacatca atgaaagata tgagttatca agctgttatg ggaagaaata
     3301 acgaaataat gaaaaatgca atcggattgg attattctta ttttgaacaa gagggaatag
     3361 ggtttgatta tgagaaaatg atgagtgaaa caggatatac attacaagat attgaagcaa
     3421 ttcaatcaca gtatgctgtt ggaaatactc ctttaataga gcttaaaaat ttgactaaat
     3481 tagctagaaa atgtgctaaa gaaggtaaag gagctagaat atttgtaaaa gatgaagcaa
     3541 tgaatgcatc tggtagtttt aaagcaagaa gagcagctac tgcagtatat catgccaaac
     3601 aaatgggata taaaggtgta atagcagcta ctagtggtaa ctatggtgca gctgtagcat
     3661 cacaagcggc aatgcaggga ttaaaatgta taatagttca agaatgttat gattctaatg
     3721 gtgtaggtca acctgaaatt atagaaaaag cacgaaaatg tgaggcatat ggagcagaag
     3781 ttgttcagtt aactgttgga ccagagttat tttatacgtt cttagtttta ttagaagaaa
     3841 caggctattt taacgcatca ttatattcac catttggaat tgcaggtgta gaaactttag
     3901 gttatgagtt agctatccaa tttagagaaa agtataaaaa agaccctgat atagttgttt
     3961 gtacaaatgc aggtggtggt aatcttactg gaactgcaag aggattgata aaagctggtt
     4021 ctattaatac taaagtagta ggagcaagtg tagatttaaa aggattacac atggctagtg
     4081 ataatcaatt taataaaaaa tcatttacaa caggacatac aggttttggg ataccatact
     4141 gtacatggcc tgataggtca gatgtaccta ggtctgcagc tagaccactt agatatatgg
     4201 atagatatgt attagtaaaa caaggagaag ttttttatac aactgaatta ttggcacagc
     4261 tagaaggtat agaaagaggt ccagcaggaa atacatcttt agcagcagca ttctctttag
     4321 ctcaagagtt agatgaggat aaagttatag tggtacaaga aacagaatat acaggcgcag
     4381 gtaaacatat atgtccacag cttacttttg caagagaaaa tggaatagat ataaaatttg
     4441 gaaatccaag agaagaaatt gcaggagtaa acttaatatt accagaaaga ccagaattat
     4501 tgaaatgtgt tgatattgat atgaataaaa taagaaagtc atttataaaa aattgtatat
     4561 ctaataatca cattgataat attgataagc taagtaatcg agatatagaa tttttaatgg
     4621 aagaagtaaa aagttcaaga gattttgttg ttgatgtatt aaataattta tagtaattat
     4681 tgttttttaa tttaatataa aataaaaatt cagaaaattc gattaaatca atacgaatgg
     4741 aggaatgttc atgaaaaaaa gagaggatga ttttgaagtt agaagaaaac atcttcaaga
     4801 actgagtgat gatgagttaa aagaaaggtt ttggagttta gctacccaga tagtagaacc
     4861 aatgatagaa cttggtaaaa agaatacaac accatcaata gaaaggtctg tcttgttgag
     4921 aatgggattt tcttctcttg aagtaaaacc tattttagaa ggggttatgg aaagaggtct
     4981 tataggtaaa ggagcagggc atgtagttta taaattggct aaatctaaaa atataacggt
     5041 tagagaagca ggtttgttat tagttaaggg tgaatattgg gatgaagtta catgtttatt
     5101 tagagaaggg gtagaatcat gttaaaggaa aataaaaaat tggatataga ctatatatta
     5161 aaagatttgg ataaatataa accaaaacga agaggttggg tttggagaga acacttggaa
     5221 aatcttgaaa tgggaccttt taaatataaa gactgtacaa aacctttaaa gaagagtgta
     5281 gggctaccat catctaagta tttcaataat atagaccctc aaccaagtcc agttattact
     5341 actgaaatag cttctggtag atttgaggat gatataagaa gaatgagaat ggcagctcat
     5401 catggagctg accatctaat ggttatcaga actgctggac aatcacattt tgatgggctt
     5461 atagaaggaa ctccacaagg tataggtgga gttccaataa caagaaaaca agtaagagct
     5521 caaaggaagg ctcttgattt tatagaagaa gaagttggta gacctattaa ttaccattca
     5581 tatgtaagtg gtgttgctgg tcctgaagta gcagtaatgt ttgctgagga aggagtcaat
     5641 ggagcacatc aagacccaca atataatgtt ctgtatagga atataaacat ggtaaggtct
     5701 tttgtagatg cttgtgaggc aaagaaaata atggcttttg ccaatatagc tcaaattgat
     5761 ggagcacata atgctaatgc tactgctaga gaagcatgga aggtaatgcc tgaacttatg
     5821 gttcaacacg ctttaaactc tatattttca gaaaaaatag gtatagataa aagtaatata
     5881 tgcttatcaa cagttccacc aacagcacct ccagcaccat gtttaaaaat agacttacct
     5941 tacgctgttg cacttagaga gttatttagt gattataaga tgagagctca aatgaatact
     6001 aaatatatgg aatcatcaac tagagaagca acagttactc atgtattaaa cttattaaca
     6061 tcagtattaa ctagagctga catacaatca actataacac ctgatgaagg tagaaatgta
     6121 ccttggcata tatacaatat agaagcatgt gatacagcaa agcaagcttt agttggtatg
     6181 gatgggctta tggatatgat agaacttaaa gatgtgggtg aattaagaga caaagcaaga
     6241 gaacttaaag aaagagctgt attatatatg gaagaaatat ctgaagttgg tggatatttt
     6301 gaatctgtgg aacaaggatt ttttgttgat tctggaaatt atccagaaag aaatggtgat
     6361 ggaatatcta gaaaaataaa aggtggagtt ggtgaaggta ctgtttatga aagagaagaa
     6421 gattatttag cacctgtaac tgctcacttt ggatacaata atgtagctca atatgatgaa
     6481 aatgctatag ataatccatc aatgttaata gatggatgta catttgaaaa tccagacaaa
     6541 ataatatata ttgatgagct tgacgatttt gataatgtag aaaatagact aaaagaatca
     6601 tatgagtaca gaaatggaac taaaattaag cctgaaatgg aatggtgtgc agatggaatt
     6661 gtaatgataa caatgatgct tcctactgat aaaagaacag cagagtttgc agctttggaa
     6721 tttgtcaaaa aaatgaactt acaagaaatt gaggtcataa gtagagaagt tatgcatgaa
     6781 tctgaaggga ctagaataga agtgaaagga agagttccgt ttgatataga tttaaacaat
     6841 ttagtaatac cagaagaacc taaggtacta actgatgaag aaataagagc agatatagaa
     6901 gaaaaaccta tgaaaatagt ttcagctacg gttggagaag atgaacattc agttggttta
     6961 agagaaataa tagatataaa acatggaggt attgaaaaat atggcataga atgccactat
     7021 ctagggactt ctgtacctgt agaaaaattg gtagatgcag cgattgaact gaatgcagat
     7081 gcaatacttg catctacaat aataagtcat gatgatattc actataaaaa catgaaaaaa
     7141 ttacacgatt attgtgtaga aaaaggtata agagataaag taatgattgc gtgtggagga
     7201 acacaagtaa cacctgaaat agctgttgaa caaggcatag atgctggatt tggaagaaac
     7261 agtaaaggca tacatgtagc cactttctta gttgaaaaaa gaagagaaat gagtaataaa
     7321 taatggaaaa tagcaaggtt tctttaaaaa tagatgtgtt ggtagctgaa attggaagta
     7381 ctactacagt ggtaaatgct ttccacgata ttaatactaa caatcctata tttttgggtc
     7441 aaggtcaagc tccaacaaca gtgtttgaag gtggcgatgt aagaaatggc ttatcaggtg
     7501 ctattaaaga tttagctaat aaattatctg tagatgatat agagtataat gatatgtttg
     7561 caacttctag tgcagctggt ggattaaaaa tgactgttca tggattagtc tatgatatga
     7621 cagtaaaagc agctaaagaa gcagctttag gggctggagc tgttattcgt caaataacat
     7681 caggtagaat taaaagaact gatttaaata aaataaaaga aataaaccca aacataattt
     7741 taatagctgg tggagtcgat tatggagaaa gagatacagc tatttataat gcagaaatga
     7801 tagcttctat gaatctaggc ataccagtta tatatgctgg taatgttgaa aatcaagagg
     7861 aaataaggtt aatatttgaa gatactaatt ataagcttta tataactgaa aatgtatacc
     7921 ctaaaataga tttattgaat atagaaccta caagaagaat cattcaaagt gtatttgaag
     7981 aacacataac tacagcacca ggaatgaaat acattaaaga aatggttaat gaaaatatta
     8041 ctccaacccc aggagctgta atggaagctt ctaaactttt atataaaaat attggagatt
     8101 tattaactct ggatgtaggt ggagcaacta ctgatgtaca ttctgttaca gatggaagtg
     8161 actatattaa taaaattttg gttaatcctg aacctactgc aaagagaagt gttgaggggg
     8221 atttaggtgt atatgtaaat atgaagaata tagtagaagt tataggtaaa gaaaacttac
     8281 aaagtgaact atctatagat attgatgcag taatagagaa ttatcctcca ataccaaaat
     8341 caaaagaaga aatattattt gttgagaggt tgacaaaaga agctgttgtt aaagctatgc
     8401 taagacattc aggaaaaata agaaatatct atggaacaac tggaaaagtc aaaattgcag
     8461 aagggaaaga tttaacagaa gtaagatata tagttggtac agggggagca cttactaggc
     8521 tccctagtag aattgaaata ttagataata tgctcaaata caataaaaat aatgaattac
     8581 tatttccaaa ggaaaaaaca aagatattaa tagataatga ctatattatg gcatctatgg
     8641 gtgttttatc taaaaaatat gaagaagcat ctcttaagct cctgttaaaa agtttgaatt
     8701 ttgaggagga aagattatgt atcctaggtt agaaatagat atagaaaagt taaagcataa
     8761 tgcaaaatta atttcgcaga tgtgtcatga aagaaatatt aagatatctt ttgttactaa
     8821 atcattttgt gcacaaaaag aaatagtaga agaaatatgt agtgaaggaa tagaccatat
     8881 agcagattca agaatacaaa acttaaaaaa tttacaagat ataaatctac ctaagatatt
     8941 gattagaatc ccaatgttaa gtgaattaga agaagttatc aattattgcg atattagttt
     9001 taactcagaa ttaggaacaa taaagaaatt aaatgaatta tgtgaaagta aaaatattat
     9061 tcataagata gttcttatgt ttgacttagg cgatttacga gaaggatact tttatgaaga
     9121 agactttttt agtaatgtaa gagagatagt aagattaaaa aatattgaaa taattggtat
     9181 agctacaaac ttaacctgtt atggagctat tataccatct agggaaaata taggcagact
     9241 ggtaagtata gcaaaaagaa tggaagaaaa atttggaata aatctagaaa tagtatctgg
     9301 aggtaattca agttctattc atctgttgat aaataataat atgccagaag gtattactaa
     9361 tttgagaata ggcgagtcca ttctacttgg tagagaaacg gcatacggtg aaaatataaa
     9421 tggaacatat caagatgcat ttaaattaat atgccaagta gttgaatgta aagaaaaacc
     9481 atcagttcct attggagaaa taggagttga tgcctttaga aataaacctg tttatgaaga
     9541 taagggaatc ttaaaaaggg caatcatagc tataggtaaa caagatataa atattgactc
     9601 tttaattcca atagacactg atataaaaat attaggggca agttcagacc acatgatact
     9661 agatgtaagt aacacaaaat atgattataa attaggtgac aatttagaat ttttattaac
     9721 ttatgggggt atcatgtcat ctagtacgag caaatatgtt tcaaaaaaaa ttatagtgta
     9781 gtgtttaaat aattaggagg aaactatttt atgaaagagc gagaatgtag aaaagcaagt
     9841 ctgtttgatg caatgatacc aattacttgt ttgatattat ttttaagtct aggagtatta
     9901 gtgtatggtt catcacctca tgtaccacta ataggagcag cagcagtagc aggacttgtg
     9961 gctgtatata gacttggatt taaatggaaa gagttagaat taagtatgtt taatagtata
    10021 aagatggcaa tgcaagcaat cttaatcata attataatag gcgtacttat aggaacttgg
    10081 atagtaagtg gagtagttcc atgtatgatt tactggggac taaaaatttt atcaccaaat
    10141 atttttttag tagcatcaac attagtctgt gcaatagtat ctcttgcgac aggttcatct
    10201 tggagcacaa tgggtacagt aggagtagct cttttaggca ttggtcaaag tttaggtatg
    10261 ccaattggat taatcgtagg ttctataata tctggagctt actttggaga taaactatca
    10321 cctttatctg aaacgactaa cttagctcca gcaatggcag gaacagattt gtttacacat
    10381 ataaaatata tgctatattc gactatacca tcattattaa tttgtttagt aatttatggt
    10441 gtaattggaa tgaagtattc aggtcaagca ctagatataa aacaaataga attaattagg
    10501 tctacattag attctacatt taatacttta tcaccagtat tattattagc tccagcaata
    10561 gtaattggat tggttgtatt taaagttcct gctataccag ggcttatagt aggtgctgta
    10621 ttgggagtat tgtttgcagt agtttttcaa ggagaatcta tgaatacgat attagatgca
    10681 gcacaaaatg gatatgtttc gtcaacacaa atacaggagg tagatgcttt attatctaaa
    10741 ggtgggatta tgaatatgat gtccactgta tccttaacaa tatgcgcttt atctcttgga
    10801 ggtattttag aaaaaacagg tatgcttgaa gtagtagcat cttctttact aagattagca
    10861 aagggtgtat ttggaacagt attatgtact atggtaactt gtacaattac caatataata
    10921 gcaggtgaac aatatctatc tatagttata ccaggaagaa tgtataataa ggaatataaa
    10981 aagagaggta tacatcctaa gatgttgtcg agagctttag aagatagtgg aactttaacc
    11041 tctccattag taccatggaa tacatgcggg gcgtatataa cagcaacatt aggtgtatct
    11101 gcactaacat atggtccata cgctttatta aatattataa atccaatagt atctttagta
    11161 ttaattgcat gtaagtttaa aatagcaaga atagaagatg aacctgaaac ttgtttaaat
    11221 tttgatgttt gataatagat aacttatgta agaacgtagg aactaggggg gtagcctgtg
    11281 gggaattagt aagacatatt ttagtgtgcg aaattctata aatcagtaat cttgtgactt
    11341 tagccatggg aggttcagat tagtaaattt atagttttat attagtctat attttgatct
    11401 tttaattgtt tgtataaatt ttttatagaa aaagatttct tttagacttg cctaaaaatt
    11461 aggcaagtct tttaagtatg ttctgattat aagagcactg tgtgacaaga atgatgagat
    11521 tatttttgct atactttcga tataagaaat ggaaggaggt tatgttttat attcatatga
    11581 aataattagt tacacagtaa attttcgtat aatttggtat aaaaagatta gaaatgaaaa
    11641 taaagtaatg gaggaataaa atgactagaa tatccaatat aacaatagct gaacaatctg
    11701 agtactgttt tttggcaata cgaaaaacaa ttgactttat ggtagagttt tcagagtttt
    11761 caaaacaaag ttttgaaaaa atttctaaat atctggagga gagaggaatt ttatcttcag
    11821 gagcaccgat tgtatgtttt cataacatgg atttagcaaa attggatgtt gaagtagggt
    11881 ttcctgtagc cacttatata gatgggaaaa atgagattct acaaaggatt gttcctgcac
    11941 agaaaataat aactgcaatt gatttaggac cttatgaatt acaagaccct actttagaag
    12001 acttattttc atgggcaaat agcaatggat ataaacttca tggcgatata tattaccagt
    12061 atttaaatga tatcaatcgt ccagctagtg aatatcttac gaaaatgatg ttacccatta
    12121 tatagaaagt taaagattat cgtttgttag ttgataatta gaagaacatt tactgctact
    12181 acataaattt tatcctaaat aatatcaagg tgatttaatt tatgtattct aatgtaaccc
    12241 caatatattg ttaaggtcta cttaagatgc tcaaaaggct atgcttaatt tattgttata
    12301 taatttgtag agtggtataa tttagaggta atattatata gatatatagg gggaaaatat
    12361 gaaaatatat gatatattaa cacgttttta tattcaagat atagaaaaag ccataccttt
    12421 ttatgaaaat cttttaaaag aaaaatgttc tttgcgtttt tcctataaag aagttgggtt
    12481 ggaattagcg caaataggaa atgttttact attatctggt tctgatgatg ctcttaaacc
    12541 atttatagaa acaaaatcta cttttatggt tgattctgtt gatgaatgga gaagttatct
    12601 attggataat ggagcagttg tagtaagaga taagaaaaaa gttcctacag gatataatat
    12661 gacattgaga catccagatg gtacaattat agaatatgtt caacatacaa agcaggatta
    12721 gttaaaaaca gattttacta ctgagcaact aattagaatc tattcttaga cgtacaattt
    12781 attatttttg gtattaacag attataagtc aaattataat atttcaaaat agaatagaaa
    12841 tttatttaca taaaagagag tgtttaaaaa tgaacttttt agttcatgaa gcactctttt
    12901 ttgtatgaaa tcaaatacat caatatataa aataaactga tgaagagctt aaattaaaat
    12961 aaaaagattc ataacaggac cccattaaac tttaagatag ggtaagtttg taatcatatt
    13021 aaataattgt aaaaagctgt ttttagtcgt ataatataaa tatttatcat ttaattaagg
    13081 aatataaaag gaatctaata agaagaaaat aataaataac tttttagatt tactaaatga
    13141 aaagttattg aaaattaaag gattagagtc tttaatcaga aagtaaaaag taatatatta
    13201 aatttatgaa ttgaaaactt aaacaacaaa tttgtttata aaatagcaag attcattaaa
    13261 tataactaag taataattga tataatgttg catggataaa aggagggaag ctgcaatgtc
    13321 ttacaaagta attaatgtca taactgctat tgactatata gaggagcatt tatcagaaaa
    13381 gctggattta gatatagttg ctaatgctgt tcattactca aaatatcacc tgcatagaac
    13441 tttcacaact tctgttggtc taacaatgca tgattacata aagcggagaa agttgacaga
    13501 agcagcaaaa ttattggtat tttcaaagaa accaattatt gaaattgcat tgatagctgg
    13561 atatgaaagt caacaggcat tcacttctat atttaaagct atgtataaaa aatctccaaa
    13621 taaatatagg aaagaacaag aattttatcc tttgcagcta agatttgttt tgaaaaaaga
    13681 taacctattt tcaaagaatg tactagagtt ggaaaaagaa attaaacttg caagtatgtc
    13741 tgacatacca ttatggatga atttagtgag attagtcata gatggatttc caaatttgca
    13801 agaggaagaa tatatatatc aattagaaca gtatatttta gaagaaagag ctttaatatt
    13861 aaaacttaat aatgtcgcta ttgcaaatat ggtgtttaat agagaaacta gaagtattga
    13921 tttttttgga atacatccac agtatcgtaa ttcagacatt gcacaagtgt ttttgaagaa
    13981 agttattgaa gatttcttga tagatacaga tatttcaatt actacctttc gagagggtga
    14041 caaagctgat acaggacata gagatatgat aaagaagttg ggctttgcag aagcagaatt
    14101 gcttgtggaa tttggatatc ctactcaaaa atttattctt ccttgtgtag aagaaagaga
    14161 taatagacaa aattaattgt agattaaatg aaaagtttac cacatatttt ttgtttgaat
    14221 tattttatat ataagagcat ttctgctttt aatgtatttt gcttgtaagt gtacttatgg
    14281 taataaatat agaaagattt agttttttaa aaaaggaact ttaaagctaa aaagctaaca
    14341 tttaagttaa atagcaagaa tgattaaaaa ctccaaaaca caattaagta taatgattca
    14401 gtgtaagtac ttaattaaca aaggaggatt taaatgtctg aaaataaaca agtagtaaat
    14461 tgcgatatta ttgcatctaa ttctaaacat aatatgtgtg aatatgatgc agagttatta
    14521 actttttttc cagatgagat tgcacatcta atagagataa ataacaagct agacaatgct
    14581 tttaagaaag ctgaaaatct tgttgacaag cttgataagg attatatgga cgctaaaatg
    14641 tatatggtaa aaaatcgtgg tgaaatagac ccacatgaaa tgtttcaaaa tgaacaagga
    14701 ctaaaacaaa tagataacta tggggctttt atggtaaaag ttcgagataa gattaataaa
    14761 atcaaagatt ctccatactt tgctagaata gattttaggc tgaaagatat ggatgatgaa
    14821 tctaagtatt atattggtag atttgcattt gattatgaag atgagcttat aatacttgac
    14881 tggcgttctc caattgccag tatgttttat gattatgaaa tcggaaaagc tggatatgat
    14941 gctccaatag gatgggtgga tggagaaata acaagaaaac gacaatttaa aattaaaaat
    15001 ggaaaattag aatatgcact agaaagttcc attaatatac aagatgatat tcttcaaaaa
    15061 gaacttagtc acacttcaga tgaaaaaatg aagtcaatca tttcaacaat acaaaaggaa
    15121 caaaatcaaa ttattagaaa tgataaagct gatacactga ttattcaagg ggtagctggt
    15181 tctgggaaaa catctatagc attacacaga attgcttttc ttctttatcg atttaaagat
    15241 aaaatatcag caaataatgt aattatccta tcacctaata aagtctttgg agactatatt
    15301 tccaatgtcc ttcctgagct tggagaagag ccattatgtg aattaagttt tgaaaatata
    15361 gcagaggtac aattagatag agttattaat tttgaaagtg agaaagaccc attagaaata
    15421 aatgatgcta aatggtctga aagagtgcgt tttaaatcta cacttgattt tgtaaaactt
    15481 attgatgatt atataaaaca aatgccaaat aagattttta ttccaaaaga ctacactttt
    15541 ggaagtttta cagctaaaag tgactggata cagagtagat ttgaggcata taacagatat
    15601 ccagttaaga agagattaga gaaggtagct gaagacattc attataaatt tgaatcagat
    15661 aatattatgg aagaagattt accaaaggta aaaagtatac ttaaaagttt gaatggaatg
    15721 ttgacaataa aaaatactct tacactatat aaggatttct ttaaacaaat gaatatatca
    15781 aacatgtttg tgatggcaac aaaaaaaact cttgaatggt cagatgtata cccatttata
    15841 tatattcatg ctgcatatga aggtatacaa gaagataaga ttataagaca tgttgttatt
    15901 gatgaaatgc aggattacac acctattcaa tatgctgtaa taaacttact ttttaagtgt
    15961 aaaaagacta ttcttggaga ttttggacaa ttggttaatc ctaatcacac tcatacacta
    16021 gatgatatga aacagctata taatgatggg gaactagtta tgttgaataa gagttaccgt
    16081 tctacttttg agattattaa tttcgccaaa aagattcaag acatatcttc attagagcct
    16141 attgaacgac atggtgaaga accagcttta ttaaaatgta ataacgagca agatgaaata
    16201 aataagataa aaatagaaat tgaagaattt aaaaagagtg ataatgcgac tcttggtatt
    16261 atattaaaga cagataatga cgctaaagct gtatatgatg cgttaagaga aaaatatagt
    16321 gttcacttaa tatcttctga aagttctagc tttacaaaag gagtatccat aacttctatt
    16381 aaaatgtcaa aaggtttaga gtttgatgaa gttattgtac cttcagtaaa taataaaaca
    16441 tattatagtg attatgaccg cagtttgtta tatattgctt gtacacgagc tatgcataaa
    16501 cttaagttga catatactgg tgaattgact caacttatag atatgtagat tatatactta
    16561 ttatgtaagt gtttacttat ataaatataa cctgtcaata aaattcatta ataaatcaat
    16621 aaaaacaata tttgtaaaag taaaagctat aaattatata ttaaatttta taatttatag
    16681 cttttataat actgcttatc ttaatcctcc caatagcaaa ataattttaa acatgccata
    16741 ttttataaat gtaataacaa ttatacaatt aaaataactt tcccaaacta acttgacatt
    16801 taaaacttac tattgtaata tttaaatatt acaatagtaa gttttaaatg tggaatcaat
    16861 tattatatca caaattaaaa ggaggacagg atgttattaa tagttactta attatcttcg
    16921 caataggttt aatcaacata attgtactag gtgaagaaaa tataagagat acagctattc
    16981 atttagtctt gtttcttata agtagaacct agattggagt atattacaaa taatgtctac
    17041 tattttaata gtaggttata aatttaaata tgggagatga tataaattat gaaaagaaaa
    17101 aagaatttta tttggatagc tatcttattg gttggtgtag ttatggtaat gtattatgta
    17161 gggaaaaaac ataacgatat aaatcaaaaa actgataaga attatataaa atctgaatta
    17221 aataagtctg aattaaataa atccaaaatt gaaaacaata ataaaaacaa aaaagaaaaa
    17281 attaatatgg ttgactatag tgattgtttt gaaggtatta gtggaggagc tattttttat
    17341 aatactaaaa ataaagaata taatatatat aacaaagaat taattgaaac aagaaggtca
    17401 ccatgttcta cttttaaaat agtttcaact ttaattggtt tagaaaaagg ggtaataaac
    17461 tcaaaagaat cagttatggg atatgatggt acagagtatc caaataaaaa ttggaataaa
    17521 aatcttagtt tagaagaggc atttaaggag tcttgtgttt ggtactacaa aaaattgata
    17581 gataaagttg atgctaaatc tgtccaaaac attttagatg atttaaagta tgggaattgc
    17641 gatattagcg aatgggaagg agatttaaaa aatggaaagg gacatttaaa tggattttgg
    17701 ttagaatctt cattacaaat atctccaaaa gaacaagtgc aaacaatggc aaaaatattt
    17761 gagggagata caaactttaa aaaagagcat attaatattt taagagatat tatgaaaata
    17821 gatgtaaatg ataaaaacat aaatgtatat ggaaaaactg gaacaggatt tgatgaaaaa
    17881 aataaacgtg tggatgcatg gtttgttggt atgctagagc gtgaaggtga cacctattat
    17941 tttgctatta aatcagatga ttccaataaa gaaattacag ggccaaaggt aaaggaaatt
    18001 gcaattaata taattaagaa atattatagt gtaagggaat aaacttaatt ttatttgtaa
    18061 atattattta cctatagtct gtaatattta ttaaataatt ttactaaatt gagtctatat
    18121 agagcaaaac tcctttagta cataaaatac ttgctattga ttattaaatt gaatacctat
    18181 agatttaaag ttaatacctt ttaaagacta tgtaatatat taaaagtata aataaaatat
    18241 attgcatagt ttttgtttta tattataatt ttaaataatg tgcaaaaact aaacaatatt
    18301 tttaatagta atttatattt gtattcatgt cactataatg tcattttagg ttgctaaaat
    18361 ttaaccataa aggagatgat tgtatatgga aatattaaaa tgtgaaaatc tcacaaaaat
    18421 atatggttca aatcaaacca gagtgacagc ccttaataat gtaaatttat cagttcaaaa
    18481 aggtgatttt gtatccattg taggagcatc tggttctggc aaatctacat tgttacatct
    18541 gcttggggga gtagatagac ctacatctgg aaagatttat gttgaagata cagaaatatc
    18601 aagtttaaag gaagaagctc ttgcagtttt tagacgtaga aaagtaggtc ttatttatca
    18661 attttataat cttataccta cccttgatgt tagaaaaaat atattattac ccatgttact
    18721 tgataaaaga aaggtggatg aggatagatt ctctgagatt gtatctatat tgggattatc
    18781 tgatagatta aaccatcttc caagtcaact ttcaggtgga caacaacaaa gggtatctat
    18841 agctagaagt ttaatctata gaccagctat tttattagca gatgagccaa ctggtaatct
    18901 tgatagaaaa aattcagaag aaattgtaga cttgttaaac ttatcaaata aacgatttaa
    18961 tcagacaata cttcttatta ctcatgatga gaaaatagca ctagaggcaa atcgtattgt
    19021 aactatggaa gatggagtga ttgtctctga aaaggtggtg aagaaatgaa tattcttcaa
    19081 gaatacacct tagactttgt acgacgcaat aaacgtagta gtatagctat aatgttagct
    19141 atattactta ctacaaccat gatgtcttct ttgtgtggac tgttgtatac catgtggaca
    19201 gattttataa aactttcaat tgagaaagaa ggtaactggc atggtgaatt gtttgactat
    19261 acttatggaa gtgacttacc attaattgaa aatttttctt cagttgatga tgtaatggta
    19321 aaaggacaat ggtatgtagg caaaattaat gatgagaaaa gagattatat aatttataga
    19381 aatgctacat cagagtattg gaattctatg ccagaaaagg atactataat agagggtcgt
    19441 cgaccaaata atgctaatga aattgcatta tcaaaacaat attttgagaa taatccaagt
    19501 gtaaaaattg gtgataaaat tactctccca ataggagata gaatcctaaa tggaaagcct
    19561 cttgaaccca tagccccaaa atcgaaaggg gaaagtttta aaaaaatttc agaggctaca
    19621 ttgacaatag taggaaaggt agatgtttct acttcatcag taatacctgc atatacagct
    19681 cttggatatc ttgataaaac aagtataaaa cctaatgata gtataactgt gtacttgcga
    19741 tttaaaaata taagagacac gtataaggaa cttccaaaac ttgctaaatc attgggatgg
    19801 aagactaatg aatatggaaa atacaatctt aaatacaata gtaattatct tttaaagatg
    19861 ttagtctttt caccagaaca gaaagcatct atgagtagta tatcaaaatt ttcaactcct
    19921 attatgtatc ttacaatagc aatttttaca gtagccgtgt ttgtaatggt gatttacaac
    19981 gcgttttcac tgtctgcaaa tgctagactt actcaattag ggatattatc tagtgttggt
    20041 gcatcaccaa aacaaattaa aaggtctgta gtgtttgaag gctttttact gacaattata
    20101 ccattaccaa taggtttatt tttaggatgg ttattgtgta ataggcttat agtttatgtt
    20161 aattcagtta attatcatac tgatgttcca gaagtggtat ttacttatgg tattcctgct
    20221 tttctgccag cagttttact tacaatagta acagtctgga tttctgcact tataccagca
    20281 cgaaaagtat caaagatatc tcctatagaa gcaatacgac aaggagatag tgttaaaatc
    20341 aaaaagtcgc ataaatattc attaggaaag atatttggaa tagaaggaga acttgcatca
    20401 aacgcactac gtgctcgtaa aaaatcttat cgtactgcaa caatatcttt aacattatct
    20461 tttttattac ttacatgttt tttgcatata attgcaaatc aagaaggagc aaaagctgtt
    20521 tatggaagtg ataattataa ggaccaaaga gatatagcag tttatttgga aaatagtgaa
    20581 cctgtagagt ctgctttcat tgaaaaatta gataatacag aaggtgtaaa aagttcacta
    20641 tattatcaaa aaatgtctgc ttccacttgg ttgactgaaa aagatgcttc aaaagaactt
    20701 gcgaaaaatg gaggatttaa aaatatagct tcatctaaaa agtactctcc tatagaacgt
    20761 gatggcaagt ttcgtataat ttcaaatata attgggcttg atgataagag ttttacagaa
    20821 tattgtaggc aaatttctgt agacccaaag ttattttatg atacaaaaaa tccaacctca
    20881 attgtatata ataaggagga agatattaat cgtagtacac gtagaaataa agtatatata
    20941 gattatttaa atctttcaat aggagataac attaaattaa atgaaaaagt atatgacgaa
    21001 gataaagggg attatactta caacatgaaa gtgggagcta ttacaagcac acttcctaaa
    21061 atagtagata ctagttcaaa gtatacatta atgcaaatag ttccaatgtc tatggtgcag
    21121 gaaatatcta aaaattatga tgaagtaagt aggttacgtt caaataggct gataggagta
    21181 tttaaagtaa ataatcgtga ggacattcct agagtaagag aaaaattaga gtccatttat
    21241 aataaagact atggttcagg agattatagt attgaggatg ttttagaaag tgaaaaacaa
    21301 aaggctagtg gaataaagac catgaattta atagtaggct ttattacagg tttattggct
    21361 atgattggat tatcaaatgt acttgctaca gtgtcaggaa atattagaag tagacgacaa
    21421 gagtttgcta tgttgcgttc tgttggatta tctccagaag gcataaaaaa gatgcttatt
    21481 ttggaaggtt tattcttagg aattacacca ttactactta gtattcctgt gcaaatagga
    21541 atagtgtatg cttttctacg tataaatgaa atatatttta tagagtattt accatttgca
    21601 cctatatcta caataatagg ttttactata ttgatacttt ttattgtaat agcatcatat
    21661 atgacaggat acaagcagtt gaagaatgaa aatatagtag aatctataaa aaatgaaaca
    21721 atataaacaa aatctatttg aacttattta aaggtttcga tttaagacta cttacaagta
    21781 tagggatagt taagttgttt gtttaataaa accttaaatg taaatagaag tatattacta
    21841 aaacaataga taacatagaa tatagatata gctggattat aggtatttta cctataatcc
    21901 agcattttat gtgtaaaatt taattatgaa tcttgcacca ccattatggc aattttctgc
    21961 atgtagagta ccattttgac gttctataat agactttgat agagcaagcc ctataccaat
    22021 actgtcctta ctagcatttt taccacgata aaatctatta aaaatatatg gaagttcttc
    22081 ctgagcaaac cccttaccat tatcttctat gataatctca acatatagag gattttgact
    22141 ataataagca actatatatc caccatcttc tatatgctca ctacaatttt taagaatatt
    22201 taaaagagct tctaaagtcc aattaatatc gatttcaaat tctaccgaag gagaagcctc
    22261 gatagtaaat gtctgatgtt tttctcttaa agaaccttct ataggctcaa ttgcataagt
    22321 aagaagagaa tgtacatcta taagtttgct ttcaaatatt aaagtacttg catccaattt
    22381 tgataatgta agtaaagatg aaactagtct ctcaagtctt gagatttgat ttgtaagtct
    22441 gttaatataa tctatttctt catctggaca attttctgaa agtaattgtg ccataagtga
    22501 catagaggta attggagtct taagttgatg agcaatatct gcaaggttat ctgaaagagt
    22561 ttgtctttct tttaaagcaa attcttttga acaacgaagt tccccaactg ttttatatat
    22621 ctcatcttca aggtgagaaa attcatcttc acatcgtaaa agtacagact cttttcctaa
    22681 attcacttct tctaagtatt gagtaagatt gtttattcta ctcagttttt gcttatttgt
    22741 tctatgtttg attatatata caactgtagc cattgttgaa attataccaa acgatatcac
    22801 tagaaaataa aaaaagtttt tactccaaaa tgtactttta gaatatccat gttttgataa
    22861 aatttcattt cctgttttaa aatccttatc actagaattt ttcagtgatt ttagtatact
    22921 ttctgtagac ttaggattgt actcatatga tgcaccaaga gccttagaca gtacattgta
    22981 ttcattaaaa gaagttatag ttgttagaat tacactcaat aaaaaagtta caattaagca
    23041 gaatattata aaaacaactg tactgttgat atttcttgga gttttcacta gttgtcctcc
    23101 attctatatc ctatgcctct taatgttttt atacaaggtg gattttttaa tttttctcta
    23161 agacgtttta ttgtaacagt aagagtgtta tcatttacga aattaccatc tacatcccag
    23221 agcatttcta gtaactttgt acgagtcata gctctatttt tattttccat aagacttaga
    23281 agaagtttat attctcctgc tgtcaaaggt acttcaacat tatctaaata tgctttgact
    23341 tcaaccttat tcaagcttat attttcacat gtaatagtat tgttagtatt tgaatgagtt
    23401 cttctaagaa ctgcattaat tctggattga agtactgaga gcaaaaacgg tttaaccaca
    23461 tagtcatctg cacccatatc taatccacgt ataatgtctt tttcatcatc acggactgtc
    23521 aaaaatatca ctggagtatc ttctatactt ttaacatatt ggcaaaactc gtatccaata
    23581 ccatcaggta gatttaaatc aagcagaatt aaatcaatag ttttatcaaa atttttttta
    23641 gcttcagcaa tcgtattata acaacataca gtgtgatttt tcttttcaag ggaagttttt
    23701 attccaaatg taattgtatc attatcttct aaaagaagta ttttagccat ttacttttaa
    23761 tcctttcagt aatttagtta ttccattaac taagttatta gttttatgtt taatattttt
    23821 catattagag atagaaaaaa ttatcattta agtatataaa ctttaattta taaacttagg
    23881 tttatgcttt aattgaatct attaaaagag caagagttgt ttcaataaat ttttcctcat
    23941 ctatattaaa gttgttcagg tatgattttc ctgttacaga tagtaaatta aaaaatcctg
    24001 taagtgtaaa tattgatgaa tacataagag taggtatttc aatatcatgc ctaatacttc
    24061 tatcattttt tccaatttca aacattcctt gtatttcatc aaacataaat ttattaaaag
    24121 aaaaaaactt ttctttaaac agcatatcag tatccgattt ttcagagttt acaataccaa
    24181 tcattcccat aaggcataat agagaaggtt ctacagagta aaatttatga aaagcaaagt
    24241 atgaaagttt tattttttca aaccctgttt tgccttttgc actttctatt ttaacattat
    24301 cccaaagtct tttatatcct cttaagacaa cagcaaagaa taaatcttct ttacatgaaa
    24361 agtacttgta tatggtgcgt ttagtatatt caacttcttt tgctaattca ttcatagttg
    24421 tattatcaaa tccattaaga caaaataatt tttctgcctt atcaataatc tcattttctc
    24481 tttgtatttt ttctttatct ctttttgata attctgacat ataaattatt cctttctatt
    24541 gataaaaaag tatactgatg gtatatttat gtaaatataa tatttgtata cctatagtat
    24601 actttaat
//
LOCUS       DAELTA010000044        24461 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.044, whole genome shotgun sequence.
ACCESSION   DAELTA010000044 DAELTA010000000
VERSION     DAELTA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.044"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            150..1067
                     /gene="pfkB"
                     /locus_tag="KPG13_001544"
     CDS             150..1067
                     /gene="pfkB"
                     /locus_tag="KPG13_001544"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0985279.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1104..3023
                     /locus_tag="KPG13_001545"
     CDS             1104..3023
                     /locus_tag="KPG13_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0985280.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            3619..5145
                     /locus_tag="KPG13_001546"
     CDS             3619..5145
                     /locus_tag="KPG13_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0985281.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            5173..5730
                     /locus_tag="KPG13_001547"
     CDS             5173..5730
                     /locus_tag="KPG13_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985282.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            6252..7289
                     /locus_tag="KPG13_001548"
     CDS             6252..7289
                     /locus_tag="KPG13_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985283.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            7400..8542
                     /locus_tag="KPG13_001549"
     CDS             7400..8542
                     /locus_tag="KPG13_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0985284.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            8995..10386
                     /locus_tag="KPG13_001550"
     CDS             8995..10386
                     /locus_tag="KPG13_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0985285.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            10639..12432
                     /locus_tag="KPG13_001551"
     CDS             10639..12432
                     /locus_tag="KPG13_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0985286.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(12513..13895)
                     /locus_tag="KPG13_001552"
     CDS             complement(12513..13895)
                     /locus_tag="KPG13_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0985287.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            14153..14500
                     /locus_tag="KPG13_001553"
     CDS             14153..14500
                     /locus_tag="KPG13_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0985288.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            14518..15552
                     /locus_tag="KPG13_001554"
     CDS             14518..15552
                     /locus_tag="KPG13_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985289.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(15601..16650)
                     /locus_tag="KPG13_001555"
     CDS             complement(15601..16650)
                     /locus_tag="KPG13_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0985290.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            16925..18460
                     /locus_tag="KPG13_001556"
     CDS             16925..18460
                     /locus_tag="KPG13_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985291.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(18645..19331)
                     /locus_tag="KPG13_001557"
     CDS             complement(18645..19331)
                     /locus_tag="KPG13_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG0985292.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            19456..20838
                     /locus_tag="KPG13_001558"
     CDS             19456..20838
                     /locus_tag="KPG13_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0985293.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            21240..22508
                     /gene="eam"
                     /locus_tag="KPG13_001559"
     CDS             21240..22508
                     /gene="eam"
                     /locus_tag="KPG13_001559"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG0985294.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(22601..22957)
                     /locus_tag="KPG13_001560"
     CDS             complement(22601..22957)
                     /locus_tag="KPG13_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985295.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(23108..24202)
                     /locus_tag="KPG13_001561"
     CDS             complement(23108..24202)
                     /locus_tag="KPG13_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0985296.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         9678 a         2730 c         4113 g         7940 t
ORIGIN      
        1 tacacctgta aatttggata atataaaact aaataagata aaaacgataa atttctcttt
       61 taattaagag aaattttttg actttcaagg atattaatat aaagttaaga tataaacata
      121 aagcaaataa ataaaaaaga ggtggcaata tgatttatac agtgacatta aatccatcaa
      181 tagattatat agttaagcta gatgagctaa agacaggaag tacaaatcgt gtaaaagaag
      241 aatacgttta tccaggagga aaaggtataa atgtatctcg catatttaag gaacttggaa
      301 atgataatac atctttaggt tttataagtg gttttactgg agaatatata ataagaacat
      361 tggaagaaaa agaattgaag acagatttta taaagataaa aagtggtttt tcaagaataa
      421 atgtaaaaat aaaggcgtta aaggaaactg agattaatgg acaaggtcca aatatagatg
      481 atgaagacat tgatacttta tataaaaaac ttgataagct aaatcaagat gatattttaa
      541 tattggcagg aagtatacca tctactttag atgaaaaact atacgaaaat ataatggcta
      601 gattagaaaa aaagaatata aaagttgtag tagatgcaac taaaaatctt cttcttaatg
      661 ttttaaaata taaacctttt ctaattaagc ctaataacga cgaattagaa gaactatttg
      721 gtgttaaatt aaattcaatt gaagacatgg taaaatatgc tagaaggtta aaggaaatgg
      781 gagctataaa tgtcttggta tcaatgggaa aagatggagc attgttgatt acagaggaag
      841 atgaagtgct tataagtgat gttccaaaag gaaaagtaaa aaattctgta ggagcaggtg
      901 attcaatggt agcaggattt atatctgggt atttgaatac tgggaaatat gattatgctc
      961 ttaaattggg agcagcttca ggtagtgcaa ctgctttttc atatgactta gccaaaagag
     1021 aatacattga taaattggta aatgagatat ctgtaaagca gttttaaaat attaaaaaat
     1081 atatagaaag gtaattaata tttatgaaaa ttatagattt attagatgaa aagtcaatta
     1141 agttaaattt aaattcaaaa accaaatcag aagcaataga agaattagta gatttggtcg
     1201 caaatagtgg aaatttaaat gataaagaaa actacaaaaa agcaattttg gctcgtgaag
     1261 aaatgagtac aacaggaatt ggagaaggtg tagctatacc tcatgctaaa aattcttcag
     1321 ttactaaagc ttgtatagca gcagcagtgt caaaagaagg tatagattat gaatcgtttg
     1381 acggaagttt atcaaatcta ttttttatga tagctgcacc agacggagct aacaatactc
     1441 atcttgaagt attatcaaga ttatctacaa tacttatgga tgaagacttt agaaataaac
     1501 ttataaattc aagctctgaa aaagaattta aggaaataat agataaaaag gaaagagaaa
     1561 aattcagtga agaatatcaa gatgagaaag ttactgaaaa aaatataata gatactaaag
     1621 agaatgatgc taataagtat cctaaggttt tagcagtaac agcatgtcca actggaatag
     1681 cacatacatt tatggcagct gaaagtttaa ataaaatggc agaaaacaaa ggtgtatcaa
     1741 taaaagtaga aactaatggt tcagcaggtg ttaaaaataa gcttacaaaa gatgaaatag
     1801 aaaatgcaac atgtataata gtggcggcag ataaaaatgt tgagatggct agatttaatg
     1861 gtaaaaaagt cataaaaact aaggtggctg atggtataca caaagcggaa gaacttatag
     1921 acaaggctgt aaatggagat gctccaattt accatggagg agatggaagt cacaacgaat
     1981 caaatgaaga aagtgaaagt ggatttagaa aagtatacaa acacttaatg aatggtgtat
     2041 ctaatatgtt accatttgtt attggtggag ggatacttat agcgatagcc tttttacttg
     2101 atgattatac tattaaccct tcaaactttg gttcaaatac acctatagca gcatttttta
     2161 aaggaatagg agataaagct tttggattta tgttacctgt acttgctgga tatatagcat
     2221 actctatatc tgatagacca gcgtttgttg taggatttgt aggaggtgct ctagcaggtg
     2281 atggtggttc aggtttttta ggtgcactac ttgcaggttt tatagcaggt tatttagtag
     2341 aagggcttaa gaaaatattt agtgtactac cagcttcatt ggaaggaata aaaccagtat
     2401 tgttataccc tttacttgga acacttttga tgggaattat aatgacattt ttagtaatac
     2461 ctccagttac agcaattaat aatgcaatgg ttggattttt aaatggttta ggtggaactt
     2521 caaaaatagt tttaggatta gtattaggtg gaatgatggc tgtggatatg ggtggacctg
     2581 taaataaagc agcatatgta tttggagttg catcacttga atcagggcag tttgaaataa
     2641 tggcagcagt aatggctggt ggtatggttc cgccacttgc tatagctctt gcaactacat
     2701 tttttaagaa tagatttact aaagaagaaa gagattctgg taaagttaat tatgtaatgg
     2761 gattatcatt tgttactgaa ggagctatac cttttgcagc tggagaccca cttcatgtta
     2821 taccagcttg tgttggaggt tcagcagtgg caggagcact tagtatgttg ttcaacgcag
     2881 ctcttcgtgc acctcatgga ggagtatttg ttataccagt ggttacccat ccatttgcat
     2941 atatattagc tatagctgta ggtgctttgg ttggaatgat gcttttagca ttacttaaaa
     3001 aaccattaaa tcaagaagta tagcaattaa aggatattca agaacttaaa atatgatgtt
     3061 ttataaaaat taatatattg ctgaattatt ttaaaataag gattttatga ggctgttata
     3121 taacgatttg taattgttat cttgacagtc ttttattttt atataagtaa aataagtgtg
     3181 ttttgcttta tttgtattta aattgttttt aagttagaat ataaagaaaa ttatatatta
     3241 ttttaatatt cactcgaact agaaaataat atttgttgta tttttttgta tataaaatat
     3301 attgtaatat taaaaaaata atagtaaact gattgcataa aaatgatttt tatataaaat
     3361 ttaagcagaa gaataaattt ctaacgaaat tggatatttc ttctgtaaat acatattcaa
     3421 aaaaagaatt ataagagttt agtcagcagt cgtgtaagta ataaggactt tataaatata
     3481 tagaaatata atgatattaa tataaccaag cgatttttaa tttagctttt taataagctt
     3541 attttttata ctatttataa taaataaact ccatgatggg aaaactatta ctatttaata
     3601 tgtaaaggag tattttaaat gaatgaatca tattggtttt taaactcaag tccaaaagaa
     3661 tataacaaat taggtgaaaa tataaaaaca gattgtctta tagtaggtgg tggaataacg
     3721 ggattgacaa ctgcatattt attagcaaaa gaaggtaaaa aagtagtgct tgttgaggct
     3781 gataaaatag gatatggaac ttctggaagg aatactggta aggtaacttg tcagcatgat
     3841 atattttact ctaagataga gaaaaaatat ggattagata aagcaaaatc atattataat
     3901 gcaaataatg aagctttaaa tttagttgaa caaataatag aagaaaacaa tataaaatgt
     3961 gattttaaaa gggaaacttc ttttgtattt acagaaaaag aggatactat aaaggatatt
     4021 aaagatgaat atcgtacatg taaaaaaata ggaataaatt gtgagtatca tgaaactata
     4081 gaaaatattc cattagatat aaagggggct attagtttta ctaatcaagg tcaatttaac
     4141 ccaaagaagt atattgatgg attggcaaaa gcagcagtaa atttagggct aaaaatatat
     4201 gaaaatacac cagtagtaga ccttgaaaaa ggaaaaatat gtagggttaa aacaagagag
     4261 gataatataa tagaagctga gaatgttata atttcatctc atagtccatg gtatgatgga
     4321 ttgaattttt attttgcaaa agaatatgca gagcgtgctt atttaatggt tgcagttttg
     4381 gaaaataaat tagcagatgg tatgtttata agtatagatg acccatcaat aacttttaga
     4441 caatataatg atggaagtga aaatttactg atttttggag gaggagacca taaagttggt
     4501 cagggaggaa ctgagaaaga aatatttgat gatttagagc attatggtaa agaagtcttt
     4561 aaggtaaaag attttaaggg gaaatggtct gctcaagata atatgagttt tgacaatgtt
     4621 ccttatattg gttatatcaa caaaagagaa gacaatattt atgtagctac agggttctct
     4681 aaatggggaa ttactaatgg aactgctgct ggaataataa taaaagattt aataataaat
     4741 aataattctg attataaaga tacttttaat ccatccagac taggtagcta cttttcaaaa
     4801 gattttataa aggaaaatgc aaacgtagca ataaattatg taagtggaaa attaaaaata
     4861 ggtagtggag atatgcctaa aaataatgga gaaggaaaga ttgttaatat tgatggaaaa
     4921 agatatggtg tatataagga tgataatgga gatttttata ttgtagacac tacatgtact
     4981 catttaggat gtgaacttaa ttttaacagt gaagaaaaaa catgggactg tccatgtcat
     5041 ggttctagat ttgactataa gggaaatata cttgaaggac cagctcttaa gccattaaaa
     5101 ctttatggac atggtgataa tgatgttaat cctaagttat tataaaatta tttttaggaa
     5161 ggtaaagtgt atatgaataa acttttaaag ccaataacta tactattgta tatagtttat
     5221 attttatctg ccgtattttt tgtagcaaac aaagaatatg gaagtctagg tctagtagca
     5281 attagtttag ttggtacttt cattttgagt tttataaata agcaaactga aagattattg
     5341 agtaaagaat tatatataat gcttgcttta tttataatgt tttcatcatt gttaggtact
     5401 tgttttaatt tttatgatat aaattattat gatgattttt tgcatttatg gtcaggtata
     5461 atatcttgta ttgtggcttt ttctataatt aaatactttt atacacaaag agagataagt
     5521 aaaatgagta aagtgttttt agttatattt ttatttatgt ttagtatggg agtagcgagt
     5581 ttatgggaaa taggtgaatt tttaatggat acttttgttg ggactactac acaggccgga
     5641 gggttagaag atactatgat tgatatggta gatgctttaa taggcacaat tataactatt
     5701 ccttttatag taaaaaaatc taatatatag ttttaaattt tttatattaa ttataatgga
     5761 tttaattaat catgttgagt ttcaaggtct atatgattaa ttaaatccat ttttgttatt
     5821 tataattatt attgaatata tttttacata taagatataa ggtgatagat agaaattgtt
     5881 ggattatgta aataattaat atataatacc attatgggca ataaatacaa atcaaatgat
     5941 aatatagtct atttatgtaa atgttatatt gtatcctaag tataatagta agttttttga
     6001 tagtggtgta gaaagtaaaa atctactagc ctaaattgat gtatggatta gtttaggaac
     6061 agtaattgca tatcttaagt ttgatgtcat gttgggctac tagaaatagg tcatcttttt
     6121 cacttgaata tcttttcttt aaacatgggg agtatcatat caaaaacata tgaaaattaa
     6181 gtgatttatg agaacagtaa tattttatat gatagtatag aatagtaata aagtattagg
     6241 aggaacattg agtgttaaaa aagtggtttg gtattgtgaa aaaaagacaa aaaagtgagt
     6301 cagtaaaaga agaaggtgaa gtaatattaa aaaacgaaaa aatattatct gaagaaaagt
     6361 tgatagatga agaaggagtt gtagttaata ttgataatca aatattaact aaagtagtaa
     6421 atgatgacaa tgaaatagaa gaaaaaataa tagaagaaga tttgttaatt agtgaaaata
     6481 ccataaaatt agatgataaa gaagcaatta ttaatgataa aaacatagaa ttatgtaaaa
     6541 aagaagttca agttgaaagt gaaaaaatag gtttaaataa gtttgaagga ctggaccaaa
     6601 atgaaaatta taatctagaa aaaaatgtta ttgaagaaaa aaaagtaagt gaatgtttga
     6661 cagaagaaga tttagagtac ataaaagaaa ttaaaataaa aagaggaaaa agtataaaag
     6721 ctataaactt gtatactaaa gaagagtggg tttttgacac tcatatacag tgtagtaaaa
     6781 aactcaaagt tccattaggg tacataagag aaaatttaaa atatggatat atggattact
     6841 ttggagatgc aataaattat ttaagtgaag tattaaatat agatgaatac tgcaaaagtg
     6901 aatggagcta tctcgataat agcaaatctc catctgagat atttaatatt ctaaacaata
     6961 aaatatttag tataaggctt tctaatgaaa aaagaaatga aattttgaca aatgataaaa
     7021 ttgaagcatt aaaaatgaat tatagatttg aatgtattga tgaagaatat gatgaatatt
     7081 ttaaaaaata taagtctata atcaaaagag gtggaaagaa aaaagttgaa ttagtaaata
     7141 aaaaaggtga cattttagaa atatttaagt ctttagaaga atgtgccatt tatttgcaaa
     7201 aggaaaagaa tgaagttata caaatgttaa aatatggaga tacaaaagta ggaagaaact
     7261 ttataaggta tagtttgaga agtatttaat ttcattttaa attcataaga gtttcatttc
     7321 tgattcacac atttaatatt attttaatgt aaaataataa aggtgaagta ttgcacaatt
     7381 aaaattaagg agggtattaa tgtctgaaga aaaaaatcca aaagaaaata cattagatga
     7441 atctaaaaat tcagataatg taaattctaa aaaagatgat gtgtcagatg aaaaaaatac
     7501 tgataacaaa gttaaaaaaa gggaaataaa gctaaacaac aaaacactta aagctattgt
     7561 agcaattgtt gcagtaggag ctatatgttt aggtggaggc tatgtttttg gcaaagaagc
     7621 tggaagaaaa ttgcctgcta cttcaaaaca ctataatagc agtgaggtag tagctactat
     7681 tggggataaa aaaataacag gtgaaatgtt aagaaagaga atggagccat tattttattt
     7741 aaatgcaaaa actactatga ctgatgacga aatacgttca tatgaatcaa atatgattga
     7801 aactatgatt acatctgata tattatcctc tgaagcagca aaagagaaaa taagtataac
     7861 taatgaccaa ctagatgctc agtataataa tttaatgagt agtatagaat cagcaatggg
     7921 aatgactaaa gaagagtttt taaaacagtt tgatttgact gaaaaatata taaaagatga
     7981 tatgaaaaaa gagctagtag catcaaaata tttaaatgaa aaatcaaaag ttactgataa
     8041 agaagcagag aactattaca ataaaaacaa gagcaattat ttacaagtta gagcttcaca
     8101 tattttaata aaaactgttg atgataaagg taagcaagtt agtagtagca aaaaagctga
     8161 acttaagaaa gaagctgaag aaatacttaa aaaagcccaa gctggggaag attttgctac
     8221 tctagctaaa aaatactctg aagatagtag tgcagaatca ggtggagatt taggtttctt
     8281 tggtaaaggt cagatggtgg aatcttttga aaaagcagcc tttgcattga agaagggaga
     8341 ggtatctaac aaacttgtag aaagtgatta tggatatcat ataattaaga aaactgatga
     8401 aaaatatcag ccatttgaag aggtaaaatc agatttagta agcagtttaa catcagaaaa
     8461 acaaaaccta cttatacaaa atctaaaaga aaaatataat gttaaaatta cagataaata
     8521 tcaaatgaaa gctaaagaat agataaagtc aatttattaa aaaaaagaaa aattaactgt
     8581 atggataaaa gctattttta ctctcaatat agagagtata aaatagcttt tattattttg
     8641 caataaaatc tatttatata tatgaagtga taaagaaata acatgcaaaa aggtattaaa
     8701 aagaaatatt tttaatttga attttttcaa taaaaattta caaaaatgaa aaaataaaca
     8761 aaaaagaatt gaagatttaa gttgttattt aaatatatat atattaagat tttctgctga
     8821 agatactaaa aaaataatat ttttcataat tttaaaaata ataataaaaa atgaaaatac
     8881 aggctataat ctattgagta aacatagaaa agatgatatt ataagtatgt gctaaaatgg
     8941 ttaaagcaat attaatatta aatttaacta taaaacaaaa gggggcgttt ttttatgtca
     9001 agtacagaaa gcgtatctaa aaaaagtggt catccgaaga gtttttggct tatttgcatg
     9061 acaattttgt gggaaagatt tagttattat ggtttaacag ctttattggt attatttttt
     9121 acagcaagtg tagctcaagg tggtttaggt ctttctactc aagaagcaac atctatgttt
     9181 ggcttattta caggtcttat atatcttaca ccattagtcg gtggctggtt agcagataaa
     9241 gttttaggtc aacaaaaatg tatttctata ggtgcacttt taatagcaat aggagatttc
     9301 gtactatttg cttcatctac atctattgca atggtttggg taggtctatc aataattatt
     9361 ttaggaaatg gtttttttaa atcaagttgc tcaaatatag ttggggatat atatcctaaa
     9421 actgatgtgt caagaaaaga tgcagcatac agtttattct atatggcagt aaatgtagga
     9481 gcatttatag ctccaataat atgtggattg ctttctgata atgtatttgc agtaagagga
     9541 gcaagtggag atatagtatc ttatggatat aagatggcat ttttaatttg tggtataggc
     9601 atgatgttag gtatgattat atttacactt cttgcaccaa aattattaaa tgaagtcgga
     9661 aaatatcctg tgactaaagg taataaaggt gaaaaaattg agtatggacc acttactaag
     9721 ttggagaaaa ataggataat agcaatggtt attttatttt tatttgtggt cttattctgg
     9781 acagcatact atcaaacaca aagttctttt atgatttatg taagggataa tgtaaataga
     9841 actctatttg gatttgaaat gccagtagca tggcttacat cactaaatgc tatattatgt
     9901 gtggctttat gcccagtatt agctacccta tgggttaaat tatctcatac caaaagaggt
     9961 gatttctcta ttccagttaa aatgggatta ggaatgataa taatgggtgt tgggtttata
    10021 gttatggtat ttgctacatt agctaatggt ccagatggaa tggtaaaggc tagtatattg
    10081 tttatagtat tgacttatat atttacaaca gtaggtgaat tattcttatc tcctatagga
    10141 cttgcaatgt tcaataaatt agctccagct aagtatgcta cgttgtctat gggagcctgg
    10201 tatctaacaa gtttttttgc aagtttatta tcaggaaaga ttgctggttt tacagccaca
    10261 cttggttttt tacagatatt tggaggaata gcaacagttt taataatatt tggtttaata
    10321 ttattgggaa taagaaatgt attaacaaga ttaatggcta tggatttact aacaaaagaa
    10381 aaataattca tgacgaatta ttgtaaataa atgaattact ttaaataaag gaagttacat
    10441 ttaaatataa aattaaatgt aatttcctct ttgattatga aaaaaagctt tacacaaata
    10501 gatattataa ttaaaattat aattgtgata attagagtgt ataagattta tataaattat
    10561 acaaaaaata tttatgtaat aaactttaca tacttatata tttagttatt caaactatta
    10621 tttcgaggag gtcttgttgt gaatataaaa gatagattaa gtggattaag aaaatttatg
    10681 gaagaaaaaa atatagatgc ctatatgatt ccatcttctg ataatcacca aagtgaatat
    10741 gtaggagatt acttcaaatc aagagaattt atatcagggt ttaatggttc agcagggacg
    10801 gttattgtga ctaaagacga agctgggtta tggacagatg gaagatattt tatacaggca
    10861 gaatctcaat tagaaggaag tacaataaaa ttattcaaaa tgggacaaga aggttgccca
    10921 actacagatg aatatttata taaaaatatt cctgaaggag gaactttagg atttgatggt
    10981 agagttatat ctgctagaga aggagctaca ttagctgaaa aattatctaa aaaaggtata
    11041 aagatagaat atcagtatga tttaatagat agtatatggc cagatagacc tgctttatca
    11101 gatagtaaag catttttatt agatgttaaa tattgtggag aaagttttag ctcaaaatta
    11161 gctagattaa gagaaaaaat gagtgaaaaa ggtacttcta ctcatgtaat aactactctt
    11221 gatgatatag catggttatt caatataaga ggtggagatg ttaaatataa tccagttgta
    11281 ctttcttatg cagtaataac tttaaaagaa gtttatctat ttgttgatga aagtaagtta
    11341 aatgaagaaa tactaaatga actagctaag gaaaatgttc aaataaagcc atataatgat
    11401 gtgtatgaat ttgtaaaaaa tatagacaaa actgaaaaag ttttattaga tggaactaag
    11461 cttagttata caatttacaa taatatacct tgtgaagttg agaaagttga tgagttcaat
    11521 ccagtaatgt tttttaaagc tcaaaaaaat gaggtagaat tagaaaatat aagaaattca
    11581 catgtaaaag atggtgtagc tttcactaaa tttatgtact ggttaaagaa aaatgtaggt
    11641 aagatggaaa tcactgaaat aagtgctact caaaaattag aggacttaag aagagagcaa
    11701 gaaggtttct ttgagccaag tttcaataca attgctgcat ataaagagca tgctgcaatg
    11761 atgcactata gtgcaactcc agagtcaaat tataaattag aggcagaagg attattttta
    11821 gtagattcag gtggacaata ttatgatgga actactgata taactaggac tacagtttta
    11881 ggacctataa gtgatgaatt aaaactacac tttacttctg tagcaagagg aatgataaat
    11941 ctttctaagg ctaagttctt gcatggttgt agaggatata acttagacat actatctaga
    12001 agttgcatgt ggaatatggg aattgattat caatgtggaa ctggacatgg tataggattt
    12061 gtgctaaatg ttcacgaagc tccaaatgga tttagatgga gagtagtacc tgaaagattt
    12121 gatagtgcag ttcttgaaga aggaatggta actactaatg aaccaggtat atatatagaa
    12181 ggttctcatg gtataagaac agagaatgaa attgtagtta gaaaagctga aaagaatttc
    12241 tatggtcaat ttatggaatt tgaagttgtt acgttagctc ctattgactt agatggtata
    12301 gcaccagaat taatgaataa ggatgaaaaa gattatttaa attggtatca taagttagtt
    12361 tatgataaaa tatcaccttt cttaactgat gaagaaagag aatggttaaa agtttacact
    12421 agagctatat agtttgtact agatacaata aaaatcgagg tattataatg cctcgatttt
    12481 ttattgtata ttttattaac tttaaaaaat ttttaatttg ataattctac taatttttta
    12541 ttcattataa gtagtataat tccaaatatc aataaaaatg atggtatcac tatgaagact
    12601 tgtaacatac caagttggtc tataatagct tgcgtatagc cagataattt atttgctgca
    12661 aatttgccta aaaaccaaac acccattagg aatgaaatat acttttcagg cgaaagttta
    12721 tttaccatac catacccgac tggagaaaag caaatttctc ctatactttg gaatgttaaa
    12781 aatccaacta cccaaaatac acttgctttg tgaattgaat tagggtctgt tcctgtttgt
    12841 ataactgcta ctatcataaa caaaaatgct atggctaata gtataaaacc taagctcatt
    12901 ttttttggca cacttaaatc gcctttttta gtttgagata attttaccca tactaaagca
    12961 gagataggtc caagaataac acaaaggaga ccattgtatg aatctatcca tgaagtagga
    13021 atttgaaaat tgccaacagt aagatttatg tgttcctttg tatagagtgc aatagacata
    13081 gaggcttggt tatatgctat ccaaaaaaat actgtgaaaa aagctaacac caatattact
    13141 attattcttt ttttctcaat caaagttaaa ggtcttgaag cgagcatttt cttatattct
    13201 agtgattttc ttatagactc tctcccgtta atagggtcat tattcaaagg tttataaaac
    13261 tttctgtttc caaatataaa gaagaataaa cctattagaa ccgtaattga tgaaaataaa
    13321 aatacatatc tatatccata catacttata gataatccgc ttttcatagc aaaccattca
    13381 ttagcaacta aaccgcatat taaagggcca aataaagtac ctagattagt agccatatac
    13441 gtaatagaga aagctccatc ttttcttggg tcatcattgg tatataagtt tccaaccata
    13501 gtatgtaagt ttcctttata aaaacctatt cctaatccag ataaaaatat catgatgttt
    13561 atatccatct tactatttgc tatgtatcct agaaaataac caattgaaac cattagcatt
    13621 ccaagtataa tacagttttt gggacctaaa aatctatcac tgacccatcc accaataact
    13681 ggagagaaat aagtccatgc taaaaaagat gcaagcattg atgcggattc tgctggtgtt
    13741 aaccctaaac cacctttgga gacttgttca gctaaaaaca ataataatac caatttcata
    13801 ccatagtatg caagtcctac aaaggtaaat attgaacagc agaaatataa atctctaggt
    13861 tgtttaagtt tttcttgcgt ttttaaaact gacatatgtc gagaacctcc agataaatag
    13921 tttaacaaaa ttttaccata ctatatgagt atatgttttt ttcatataga cgtcaacttg
    13981 taagataatt taattaagtc gaaatatatg taaaaatagt atcatttttg tgtattcagt
    14041 aatatttttt tatgataata tatgttatta ttatattaaa atacaaacat atttatggaa
    14101 aggatatgcc aatttagtag ttagattgat tgtttggcag gtgggatgag ctatgataag
    14161 aaagaaatct gaattacaag tagaagtaaa agaaaattta aatggtggta taggaaaggt
    14221 taagcttgaa aacataatac aaaatgaaga gcttaaagga aaaggaagac tatttaaaag
    14281 agtagcttta ccagtaggaa gttcaattgg tgtgcatgac catacaacag attttgaagt
    14341 atattatata ctaaagggaa aaggtaaagt atttgataat ggggaatttg tagaagtaaa
    14401 tgagggtgat gtagtgtata cagctgatgg cgaaaaacat tctatagaaa atataggaga
    14461 agaagatctt gagtttgtag cactagtttt atatatgtaa aatatggagg aggagtcatg
    14521 acaggcaaga tagaagaaat taaggacaag ataaatagat tttgcaactc caatttaaat
    14581 caagaataca aagatataag ctttaaaatt ttgcaaaacc ttttagataa taataaagag
    14641 ataattcatt cttctagagc tgatatatgg tcttcagcta tactaaatat agttttagaa
    14701 caaaatcttt tatacaataa gaagcatcca ctacatataa caaaaaaaga gttttcaaaa
    14761 caaataggtg tttctttaaa tactataaat aataaatcgt ctttaataaa agatgtttgt
    14821 agtattgact ttttaaatca agatactata gctatttcta atgtagaatt ttgggtaaaa
    14881 gagactaatt ccaaatcaaa gtctgtagat ttagatttag aaaagaagaa aatactatat
    14941 aaaagatata ttaagctatc acaagatgcg aatagtgata ttgaatcaat tagatatatg
    15001 gaggaagctg taaatatagg taaaagtatg attactaaag aagataaaga acttggattt
    15061 tggaaaggtt tatctaccag agcctatatg atagcacttg aatcccttgc tagaaagtta
    15121 gaaagtatta ataatctaaa ggagtccaaa aaagtattag aatatttaat agaaattaat
    15181 cctagtgatg agcagggaat aagatacaaa ttattaaatg tattaattag attaaatgat
    15241 agaacttcaa taaataaatt atttgaactt tataaagaag aaaaatctgc tacatggatg
    15301 tacagtaaag ctctttatta ctttaaaaat aaaagtatgt ttttggcaaa tgattctatt
    15361 aaggttgcta gaagtaaaaa taagtacgtt ggattatatt tgttagattg gcgaaatgct
    15421 tttggtagag aatttgttac agaggaagaa aaggcagaag ctgtctatta ctatgatgaa
    15481 aacatagtga tttggaatga agttaaaggt tctatggatt ggctattaaa aaaaatgttg
    15541 gaattttcat aaaaaaaact actccttact tttatataag tcaaaggagt agtttttaat
    15601 ttatacatct agttttaaat cttcaggttt tattagacct ttttttctca taaattctga
    15661 aatcaacaaa gttacaatag caggaagtat aaaattaagt aaaagtatac caacccacat
    15721 agatatacct ccttttccag cactttgcat agcagttact gttccaaatt gtccaaccaa
    15781 tccacaagta cccataccag aaccaatagg aatattctcc attttgaata ttgttgtaga
    15841 taatggtcca agtaatattg aagctacagt aggtggtatc caaattttcc aattttttat
    15901 tatattaggt acttgcaaca tagatgtccc aagaccttgt gccacaaggc caccaactcc
    15961 attttctcta aagctcataa cagcaaagcc taccatttgg caacaacaac ctacagttgc
    16021 tgcaccacca gcaagaccac ctaaacttaa catcatacaa atagctgcac tacttatagg
    16081 taatgtaaga gctattccaa caagtgcaga gactaaagct cccattatta taggttgcat
    16141 agttgtagct tgcattatta tatttccaaa tgaagtcata aaagcagaaa cagctggacc
    16201 aataagttca gctacaagta ctccaattag tattgttaca gcaggtgtaa ccaatatatc
    16261 tatttttgtt tcttttgaaa ctaattttcc aacctctaca gcaaaaagtg tagcgacaaa
    16321 tgctccaaca ggtcctccta attcattacc acaagctcca acaagtgcac aagaaaatag
    16381 tacaagtgga ggtgcttgaa gtccatacgc aatagccact gctatagctg gaccagtaac
    16441 ctgtttagca agagggctta taacttcagt aaaaagagaa atattgaaat tatctcctaa
    16501 tgtattgaaa attgttccaa ttaaaaggga cgaaaaaaga ccgagtgcca tataacttag
    16561 tgcgtcaata agataccttt gaacagaaat ttcaatattt tttcttttag caaatgattt
    16621 gaaatttctt tttttataat tgatttccaa tttaaaatcc tcctcatata atataagtat
    16681 aatttctttt taattaatta tataactttt cagctgacaa gacaaatgtt atttcactaa
    16741 aattagacta ttgtaatagt aagaaattaa cattaatttt agttttaaat tttaaattta
    16801 tggtttataa ttagtatagg gatattctga aatataataa ttttccagtt tatcccaata
    16861 tttaacttag gtagattgct tacaaaacaa atgattatat attaggctaa aaggaagtgg
    16921 atagatgata tttgtaaaaa ataagaaata cagaaaaatt gccataatta tatctattcc
    16981 tatattgctt gttgcgcagt actacctttt taaatttgga atacttagac caatggtaaa
    17041 gggagtagaa attgaaattg tagatgggga atatgttaaa gatatagata aatatgtagt
    17101 aaaattaaat gaaacagtta ctttgtctac aggtgaatat attaaggttc cttcgtatgc
    17161 taaaaatcca aatatttggt ttaatgtatt ggataactca gggactttaa aaatagatgg
    17221 aaataaaatg acagcattaa aggttgggta ttcttctgtg ggtatcatga aaaattcaag
    17281 agtattaaaa aaggctacta taaaagtagt agacccagag gtagaatcac tagatatgga
    17341 gattaatggg gatttaaaac atgttggtga tagtgctact atagaaagta caatagaagt
    17401 tgattatgat aagtttaaaa aatcatataa gccagtttat aagagtagca atgaaaatat
    17461 tttaaaaatc aaaggaaata aagtaagtgc agttggagtt ggtaaagcta caatatatgc
    17521 taagtgtgga aataaagaaa ttgagagaac atttaggatt caagctagag ttgctgaaat
    17581 aaatatggat gatataaaaa tagaagttga ccagaatgaa aaattgaaac cagatataat
    17641 aacttctccg cctggtttag aaccaccaaa aatatattat gagttagtag attcaaaatt
    17701 acctgtagaa agagcaataa gtttaagtag taatggaaat gttgtagggc ttagagaagg
    17761 taaagatagg gtgagaataa cttgtggaga taaatctaaa gtaataacag taacagtagt
    17821 caaagaaact ataacgaatg actatataca aaacttggtt ggttcacata aaatagtaaa
    17881 caataaagtt attattaaac ttgaatggga ttatatgaaa gatgtctttg attatgatat
    17941 atatcttaaa gataagttgt caggagaatc atttaagaaa attaaatcta taactataaa
    18001 tgaaagtgat ttaggtaagt ctaacaaggt atatgcaact atagaagtag atatgaaggg
    18061 taatcaaaat atagattttg atatttatgt catagggaaa aaggattcag atacaactaa
    18121 gccaagtaat gtaataaata ttaccaatgg aaatggaaat tcaaataatg ataataatat
    18181 taaagatatg agggtagaaa atatatcagc taatgttgat agagataata atgtcattag
    18241 agttaactgg agtagtatat ctcataagga ctgtacttac agtatatatg taaaaaataa
    18301 tactaatgga gatggagaat ttactctata tcaaagtgat atacagggaa atgagcatac
    18361 tataagtatt cctgaaggag atttaaatct agatatttat gttgttgcaa attataatgg
    18421 aaatagctct caaaatagtg atgttataaa tgtaagatag atttgtatac aaattgaaag
    18481 tgttgagttt atttaaatat taaattaaga aaaaggatag tatagggtaa ttatactatc
    18541 cttttttgtt acaatatata ggtttaaaat ctctagtaat ataaattata aaaagtctta
    18601 tttacacaaa tctatagata ttttttttat taatcaatat tgtcttaaat tgaacttagt
    18661 gcggcttcta ttgcatgtat tactgtcgta tcaaatagtg gaatagaagt gtcattttgt
    18721 ttaattaaaa gacctatttc tgtacatcct aatataacag cctctgcacc ttgtttgcca
    18781 agcttatcta taatagataa gaaagctttt tttgaagatt cagatataat tcctaagcaa
    18841 agttcattaa atattgtgtc atttactatt atacggtctt cttcatttgg aattaagact
    18901 tcaattccat tattaataat tacattttta taaaaatctt gttccattgt atatttagta
    18961 cctaataagg ccactttttt tatttctttt tcccttaata ccgtagcagt tacatctgct
    19021 atgtgaagta atggaatatg tatactttct tgtattttat ctgatacctt atgcatagta
    19081 ttagtacaga taacaataaa atctgcccct gcctcttgta atttaattgc agcatcagct
    19141 aaaattttgg cgcttttttc ccaatttcca ctagattggc attcttcaat ttcttgaaaa
    19201 tctacactgt aaagtataca tttagaagag tgtagtccac ctaatctttc ttttattact
    19261 gtattaataa cttgataata tgtaatggta ctttcccaac tcataccacc aattaagcct
    19321 atagtcttca tattatacct ctcttattta tttattttga aatagctatt tacaaataca
    19381 agtatagcat ctaaaataga agcatacaat gataaaattg tatggagtac aattataatt
    19441 aagaggagta attatatgcc aataaattct tttgaaaact atcctatgac atggaaacca
    19501 aaattagata accgaaaacc accaatatat aaaactttag caatgttatt agaagaagat
    19561 attaaaagag gaaatttaaa tcctggagat aaactgcctc cacaacggga attagcagat
    19621 tttctagatt taaacttaag tacaataaca cgagcattta aactttgcga agaaaaggga
    19681 ctaatatgtg caaaagtggg aaagggaaca ttcatttctt ctgatgtaaa tgtaagtaat
    19741 acattattat atcaaacgga atcaaaagac ataattgaat tagggacagt tcatcctccg
    19801 tatgagcaaa atacatatat tattgacttt attaaaaatg tactaaaaca accagaaatg
    19861 gataggtttt tacaatacat gtctccatct ggcacatata tgcaaaaaaa gtcaattgca
    19921 aaatggatag aaagaaataa tgtatacacc agtgaagaaa atatattatt atctacagga
    19981 gggcaaaatg ctatatgtgc aactttacta ggattattta aagctggtga tagaattgct
    20041 acagattctt taagtttttc tggaattaag tcaatagcta aaatgatagg tattcaatta
    20101 gtcccaattc ctcaagaaaa taatgaaata tcgattgaat atcttgaaag ttattgtaaa
    20161 aatgaaaata taaaaggcat atatcttata ccagattatc ataatccaac gacacatact
    20221 atgtctgatt tatctcgtaa aaagatagcc aagatagcaa agcaatataa tataataatt
    20281 attgaagatg ctataaatag catttttaga gatggtatac aaactcccat attttcttta
    20341 gctagtgata atactatata tatttttagt acatctaaat ttttatgtgc tggtcttaga
    20401 gtagcatttg tagttgctcc aaaacgatat atagaaaatt tagaaaatgc actatataat
    20461 atgaatctaa tggtttctcc atttacagca gaaatagtac accgtttatt atattctcct
    20521 attatagata aaattataga agagaaaaga gatgcaatta tagaaagaaa tgaagttgct
    20581 gataaagttt tgtcagatta taatttgatt ggtgataaga attgcagttt tcgttggtta
    20641 cttcttcctg atgaattgga tggaaaatca tttgaaattt ctgcaaaaaa tctaggagtt
    20701 caagtatatt gtgctgaacg attttcagta ggaaactcta cagttccaaa agcagttcgt
    20761 atttgtgtaa cagctccaaa agatgtagaa gaattagaaa aagggcttaa tataataaag
    20821 tcacttctta agagttaaat ttattttact attatttaat taaataaata aaaaggcgga
    20881 gaaatttatt ataataaacc tccgctttta ttatttaatg aaatacttta ctttttatta
    20941 aaaaatttaa ttttaataat cttgaaaata taatcgtttt atggtaaaat aattcttaaa
    21001 aatagaaaaa attaatcatt tgttaaaaaa aatgcataat ttattgtaat ttattgacga
    21061 aggttgaaaa atattcataa atgtgcgaaa taacagaaaa aatgcaaaaa ttaattcaat
    21121 ttcatttgtt ataaaatcta tatggcaaaa ttaaatctgc ctatcagaat gatataaaca
    21181 caccagaaag gaggacgcta tcaaataata caagtagtta actttgatag caaaaaatta
    21241 tgaatgaaca aactagaata tccttagaga gagctgctga attaaaatca aaaattgatg
    21301 attatattca ggctagaaaa acgattaaca gaggtcttga aaaagaagaa gagataaata
    21361 aacgaaaaca gaaaatatta agtatcttaa atggaactga agaggattgg aataactaca
    21421 aatggcaatt atcaaataga ataacagatg tagatacttt atcaaaaatt ataactctaa
    21481 ctaaaaaaga aaaagaatat ataaaagagg ttggtactca atttagatgg gcaatatctc
    21541 catattattt gagtcttata gacccagaag atacatgtga cccaataaaa ttactgtcta
    21601 taccaacaca tatagagttg gaagatgaac aagaagattt ggacccaatg ggagaagagt
    21661 atacaaaccc agcaggatgt ataactagaa gatacccgga tagattaata ataaatgtaa
    21721 caaatgagtg tgctatgtat tgtagacact gtcagagaag aagaaatatt ggacaacaag
    21781 attctcataa gtcaaaagct attatccaag aatctataga ctatatcaga gaaaatgaag
    21841 aaataagaga tgtactagta actggtggag atgctcttac attaaaagat gattatttag
    21901 agtggattct tagccaactt aaagagatac cacatgttga ttatgttaga ttaggtacta
    21961 ggactcttgt tacaatgcca caaagaatca cagatgaatt ttgcaatatg ctaaaaaaat
    22021 atcaccctgt atatataaat actcatttta atcatccaat ggaaataact aaggaatcta
    22081 aagaagcttg tgaaaagtta gcaaatgcag gagttccatt aggaaatcag gcagtattat
    22141 taaatggaat aaataatgat aaatttgtaa tgagatgttt aaatcaagaa ttactgaaaa
    22201 taagagtaaa accttattat atattccaaa gtaaacatgt aaagggaaca aaacatttca
    22261 atacatcagt agatgatggt cttgaaatca tggagtattt aagaggatat acatcaggaa
    22321 tggctatacc aacatatata gtaaatgctc caaaaggagg cggaaagact cctttgcttc
    22381 ctcaatacct tgtatcaaaa ggaacagatt acgttatgct tagaacatgg gaaggaaaag
    22441 ttataaaaat ggaagatgaa cctgctgtag atataaagaa acttataaaa gaacaagcgc
    22501 aggattaaaa aagtatttaa agtttataaa aagaaacaac tttaaaataa aaagaaaatg
    22561 gatgtcaaat ttatatcaat ttgcatccat tttttattag ttatttatta ttttgttgtt
    22621 gattaactaa ttcaagatat ccacttgggt ggaagtaata tatctcagaa gtattttcat
    22681 ttacaaagta accaatatca ccatctgcat ctggtaaaaa tacataacca ttaagatttt
    22741 tagcttttaa tgcttcaaaa tcactagcat ttccttgata tatgtaatca acttcattgt
    22801 tgtattttac taaaagttct ttagcttgtt cttgagtcaa tttagtattt tggcttacag
    22861 gaacattctt ttgagaatct gcataagaag attcatttac tggtgcagtt aataacaaac
    22921 aagataaaac taataaagat ataagtgctt ttttcatact aaaaccctct ttcatgttta
    22981 attatataag attcattact taataatatt gtatgtttcc tataattaaa tattaccatt
    23041 aaagagaata atattacagt tacacagctg taacttttga ttacaaaatc gtaattaaat
    23101 tgtattttta ttccatttgt attttatcat gtctacgatt tacaaactca cagaagtata
    23161 tctttataac gcccatcaca ggagcagata gaatcatacc aggtatacca aagaatcctc
    23221 caccaacagt aactgctagg attgtaaaga atgggctaag acctagttgt ccacctacga
    23281 tttttggctc tataaatgta acttcaactt gttgaattat tattaagtaa attaaagaag
    23341 ttaaagcaat tgttggattg gagaataaat taattattac aactggtgcc atacctatta
    23401 ctggaccaaa atatggaacc atattcatga cacccataag tgtaccaaat aatagtgcat
    23461 attcagattt taaaaagtaa agaccaattc cagaaagtac tccaactata aaagaatcta
    23521 aaatcttacc tgtaaagtat ttaccaatat ttaaatttaa gctttgacat agttctatta
    23581 taaagtctcc atatttttta cctaaggaaa tataaactac tttttttgta aacagtaaga
    23641 atttttcttt ttctaataat atatagaaac aaattataaa acctaaaaga aattgtacta
    23701 taaattttcc aactgaaaat gtagtgctaa atatcccacc aagtgaacca ataagcaagt
    23761 tactaaattt aggcattaca ctcataattt tgtcaccata ctcttttaat gtattagggt
    23821 caactgattg taaactcttg cctaagtcga ataaaaactg ctctgtttta tctatgtatg
    23881 taggaatttg agcaactaaa tcagctaaac tatttactat ggatggaact gtaaataata
    23941 taaacgaaat tagtataact atgagtacac cataagtaag aagtaacgaa tatattctct
    24001 taaaatttaa tttactttct aagaacttta ctagtgggtt gaatatatat gctaatacaa
    24061 atgctattat aaatggtgtt agtagtgaca ttaataaact tatcactcca aaaaaatatt
    24121 tgtaattatc aataacttta atcaagatat atgaaacaac tacagtgagt acaatattta
    24181 gataaatttt tttatcttgc aaaataacac aacctttctt atatttatta aatttagata
    24241 tacttaaatc taagttaaag ttaatattta tagcttatta tacaataaaa agcagtatta
    24301 atatagtatg attaatatga aattataaat acgttaaaac tttatgttta tagctaaaat
    24361 aataaaaatt atttatattg tgtttatata taatctaaaa tgaaaaaaag tggtgccttt
    24421 gattaaacta agtttttata atttaaattt tgtacaataa g
//
LOCUS       DAELTA010000045        23769 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.045, whole genome shotgun sequence.
ACCESSION   DAELTA010000045 DAELTA010000000
VERSION     DAELTA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.045"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(259..1689)
                     /gene="dcm"
                     /locus_tag="KPG13_001562"
     CDS             complement(259..1689)
                     /gene="dcm"
                     /locus_tag="KPG13_001562"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBG0985297.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            1902..2618
                     /locus_tag="KPG13_001563"
     CDS             1902..2618
                     /locus_tag="KPG13_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBG0985298.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(2863..3141)
                     /locus_tag="KPG13_001564"
     CDS             complement(2863..3141)
                     /locus_tag="KPG13_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985299.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(3389..4723)
                     /locus_tag="KPG13_001565"
     CDS             complement(3389..4723)
                     /locus_tag="KPG13_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0985300.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(4736..5479)
                     /locus_tag="KPG13_001566"
     CDS             complement(4736..5479)
                     /locus_tag="KPG13_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985301.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(5500..5814)
                     /locus_tag="KPG13_001567"
     CDS             complement(5500..5814)
                     /locus_tag="KPG13_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985302.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(5818..6012)
                     /locus_tag="KPG13_001568"
     CDS             complement(5818..6012)
                     /locus_tag="KPG13_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985303.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            6296..7012
                     /locus_tag="KPG13_001569"
     CDS             6296..7012
                     /locus_tag="KPG13_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0985304.1"
                     /translation="MIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFD
                     NYSAKLIGNNISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALAT
                     YTKIEDLFGFPLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDK
                     SEFDLVIHILKNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGL
                     VENFIQENSNGLIEDEDEEY"
     gene            7109..7861
                     /locus_tag="KPG13_001570"
     CDS             7109..7861
                     /locus_tag="KPG13_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0985305.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            7851..9017
                     /locus_tag="KPG13_001571"
     CDS             7851..9017
                     /locus_tag="KPG13_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG0985306.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(9134..9364)
                     /locus_tag="KPG13_001572"
     CDS             complement(9134..9364)
                     /locus_tag="KPG13_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985307.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(9613..14184)
                     /locus_tag="KPG13_001573"
     CDS             complement(9613..14184)
                     /locus_tag="KPG13_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG0985308.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
     gene            complement(14589..15503)
                     /locus_tag="KPG13_001574"
     CDS             complement(14589..15503)
                     /locus_tag="KPG13_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HBG0985309.1"
                     /translation="MELVKNIHKNKSKDEIFKELLENLKGTIASWDYYVNWNKALENT
                     RKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVIPIVIASRDNEFKILDPSDEDM
                     FKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQKNIKNLVDYVFGVEVGLDTNA
                     RKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKISEKWSIEVPTDKSKRKYDFAI
                     NNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKDGGFTFCWITDGLGWLESSIPL
                     RDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(15522..16436)
                     /locus_tag="KPG13_001575"
                     /pseudo
     CDS             complement(15522..16436)
                     /locus_tag="KPG13_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(16864..17213)
                     /gene="ssrA"
                     /locus_tag="KPG13_001576"
     tmRNA           complement(16864..17213)
                     /gene="ssrA"
                     /locus_tag="KPG13_001576"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(17371..17826)
                     /gene="smpB"
                     /locus_tag="KPG13_001577"
     CDS             complement(17371..17826)
                     /gene="smpB"
                     /locus_tag="KPG13_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG0985310.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            18432..19259
                     /locus_tag="KPG13_001578"
     CDS             18432..19259
                     /locus_tag="KPG13_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0985311.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            20284..20748
                     /locus_tag="KPG13_001579"
     CDS             20284..20748
                     /locus_tag="KPG13_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0985312.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(21015..21659)
                     /locus_tag="KPG13_001580"
     CDS             complement(21015..21659)
                     /locus_tag="KPG13_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985313.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(21589..22878)
                     /locus_tag="KPG13_001581"
     CDS             complement(21589..22878)
                     /locus_tag="KPG13_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985314.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(22875..23747)
                     /locus_tag="KPG13_001582"
     CDS             complement(22875..23747)
                     /locus_tag="KPG13_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985315.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         8162 a         3807 c         2531 g         9269 t
ORIGIN      
        1 acatttctaa agttagacat acatctttaa aaacctttaa tttgtgctac tttttttcta
       61 aaaataataa atatataaaa attatatata caaaaaatta agttatgact agtttataaa
      121 taagtacata ataaaattaa agagagcaat tttttttgct ctctttcaaa atttatatta
      181 aattgtaata ataaaaataa aaacgctagt tatctaaggc taatattttc aacgattata
      241 taaatatcta cataacagtt aaattacata ctcttcttca gttattgcaa cttcctcagt
      301 gacatatatt ttttctattt tattaagata atctactata cttaatccaa tagcctttcc
      361 tagcttaaca ggaactgcat ttcctatctg cttgtacacc tgactaactg accctgtaaa
      421 ctcccactca tcaggaaaac tttgaattct agcatactct ctagttgtga atggtctagt
      481 ctcatctgga tgacaccttt cagtttgctt ttgtgcagga ctacatgtta aagttaggca
      541 tggttcatcc caacttattc gccttgccat acctcttttt ccgccaccgc tataataact
      601 ttttcccata taatctcttg ccacctcatc tggcaaatct atccaacacc cacctggtgg
      661 taccatatct aacacttttt tcttcctctc aggatatcta gctccttccg attttggaac
      721 atttttaagt gcatctctta acgtagcaac atattcaaac gacttaggga acttaaaatc
      781 cacatgtgac aaatcttttc ttgttcctat tataataact ctttccctct tttgtgcaac
      841 tccatagttc acagccttta gtatctcata ttttacattg tatccaatct cgctaaatac
      901 atctatcata gtctttaatg tttttccacc atcatgagat acaagacctc taacattctc
      961 tgctaaaaac accttaggct ttaactcttc tagaatttta gcatatgaat aaaacaatgt
     1021 ccctcttaca tcctctaacc ctagcttctt acccgcatag ctaaatgctt ggcatggata
     1081 accacctgat agcaaatcta tctctatatt attatcaata taattcttta ttccattttc
     1141 cgctatttta acaatgtcac cctgtataac attccagtct ggtctattta attttaatgt
     1201 atcacaagca tatttatccc attctactaa tccaacctct ttaaaaccag ctttttcaag
     1261 gcctaatgct aatcctcctg ctcctgcaaa taattcaaca catgtatatg ctcttttagc
     1321 tagtacatta cttatatcta tatgtttatt atttttattt ctctctctag gatttaattc
     1381 atattcactg agttctttat atgtacagtc tctatgctct atataatctt tattactgct
     1441 acaaatattt gtaaatttca gttgcttttc atttacatct tccaaacaca taatttcatc
     1501 gaatccaata tctaacactt cacataattt ttctacatta ctttttatag gtgtaaattt
     1561 ttctgataat attgcagata actgattctt agttatgccc atatttaatg ccagttgatt
     1621 ctgacttctt atatttttct ctttcataat ttttctcact ctatttttat caatagttat
     1681 ttttttcatt tagttcacct cttaataaat aatataccct taaatagtta ataaaataca
     1741 ttaactattt aaggatatat tatcataatt ctaattttta tgtaagtagt tataaaaact
     1801 taaaataaaa tatctgtaaa caacctaata ttacctcata attttacaca ttatggaata
     1861 tattgtataa ttacattaat actttttagg gggattattt tatgactgaa ttagaaaaac
     1921 aagaaatact agataaagcc aaagattttt ttacaaatat tttggttgct aatcataaaa
     1981 agaatactat taagttaaat ttaaaggcat ttaatataaa tcctttttta gttaaatact
     2041 tagctagttt cttaacagga tcaaataaac ctgaagatat tgctaaagca ttgatttatc
     2101 caagagttct aggcacttcg attaatacgt ccttcggaac taatttacaa aaattctgta
     2161 ttaccacttt agatggtttt gcctctacaa cttctggcat tgatatagag tttattgatt
     2221 gtattgatgg tagaaggaaa ttctgtcaaa ttaaagctgg accaaacact attaataaag
     2281 acgatgttaa aactataatt gaccacttta gctcagttaa aagacttgct agaacaaatg
     2341 gactttcatt atcttataat gacttaattg ttggtgtttt ttatggaaca gaaaatgagc
     2401 ttaatgctca ctataaaaga attaatgaag agtatccagt agttgttggt caagattttt
     2461 ggtataggct tactggtgat gaaaattttt attttgattt aatagataca attgctcaat
     2521 ctgctttaga agaggactgt actgttttac ttaaaaaaac aatatccaat ctagctgatg
     2581 aaatttctaa agataaatct ttattcaatc aaaagtaata taaaaatgat taaatagcat
     2641 aatttacatt tttagatatt taaaaataat attcaccaaa caaaattatt aagtaaaatg
     2701 aaatgtatct tataaaagct gagctataag taaatctttg taaaaacaaa tttacttata
     2761 gcttttttat gttcaaaaaa cacaaattat atactattta atatattata aacttttatt
     2821 tgttaatact ctctactaaa tttctaacat ctcttatata aattaactat ttaataattc
     2881 aatcataaat ctagctcctg ccttaaaacc aagcttataa ccaagctctc tttcaatctc
     2941 tataactgaa ttaaataaat catttaactc tatcaataca tccattttct tttcttctga
     3001 taaatttttt tctagtaatt tattaacttt cttaatttct ttacatctat cattataata
     3061 ctcatctttt tctagtactt tcctatacat gtgttcattt atccaataat caaaatatat
     3121 ttctataatt tcatcataca tgttagtacc tccttgactt tcatctggta cttaatatat
     3181 acttaacttg tgtagattag tgtacattaa gtacctaatt tataaaggta gttgttatat
     3241 ttatatggca actacctttt tctaataaat ttttcaatct aaatactata tattttaaat
     3301 cttcattgct tactattgag gattatatac ttttcttcta acactcctta aacaatagtt
     3361 tatatatcac ttaataaatt atattttttc atacctcctt taccttctta cattccttaa
     3421 aagaaaacaa acttcctata taccaataag gtctaaagcc tgattcccca tctctatttt
     3481 tagcaactat aatttcactt aaattagctc caaaaccttt ttctctacgt cttaattctt
     3541 ttatatcctc tattcccatt gtcttacaat aatcaagagc ctctttctct gtaggtctat
     3601 gaatatatat gactgaatta gaatccatat atatctgttt tgaacttctc attactctat
     3661 ctccttttgg cctttcatcc ttcaattcat catttaattg agccaactgt ataattggaa
     3721 tattaaagtc taaagtaatc ttttttattt gccttgataa atcagcaaac ttagcttctc
     3781 caacttcttt accttcgtaa tccaaaatct gaagataatc aattataaca acatctggtt
     3841 taatactttt tatttctctc ttaattcctg atattgtatt gactgaatca ttaatataga
     3901 gattattttc ctgatttaaa gtacttaaag catttatgta tttaattatt tcttcttcat
     3961 ttagatttct attcttcatt tttacaactg atattccagt taaagaagct atgtttctaa
     4021 acaaaatttg tatatcactc atttccccac ttataaaaag tactttttta ccttgaaata
     4081 ccacatttct cattatctgt aaggctagtg ctgtttttcc tcttccactt ttggctgcta
     4141 ttgtaattaa ttcaccacta tatagacctc caatagtttc atctaaaaat tttattccta
     4201 tatcgatacc ttcttcaatt tcatcattta tgtattcaaa ggctctttct gatatagctg
     4261 ttatactaga atccttctta accaatttct gattaacttc attaacgtat ttttctaatt
     4321 gatatataag tgtattatca tctatttcac ctaatttgaa tttattaaat ttatcaatta
     4381 tatttctatt tttatatttt tcaataacta tctcaatata atgggataca ttacctatag
     4441 ttggaactat tgttgatagt tttgtcaaat aacttatacc tccaacataa tctataagcc
     4501 cctttttctt taaatcttct gtaagagtta ttatatctat aggctcatcc cttttatgta
     4561 tatctttcac tgaattgaat atatgtttgt gctcttgatg ataaaacata tcttcactta
     4621 ttaatatctc aatctcttct attttcctag aatctagtaa aatactacct aatatagatt
     4681 gttctgcttc tgtgttctta actaaattac tcatattatc cataatataa actccctacc
     4741 actttaattg tgaatcatct ggactatatg cttctgcatc tactttgaca ttaattgatt
     4801 tatcttgata attgccttct aatatcttaa taaaattatt tgggctcatt atccaatcaa
     4861 aatttgcctt ccaacctttt tcattgcttc catttaaaaa gctagtttta ctaactatat
     4921 gaataatttc taatacagtc tctttatcat attcttttat tctagcatta attacttttt
     4981 ttctagcttc tgtaagtttc tttacttttg atagttctgt acaagtaata ttgaattttt
     5041 ctataatttc actgtatatt ttactttttt cattatttac attcttgtta gttgttagtt
     5101 gcttgttaat tgcatgttga ttagtctgtt gattttcttc attagattga tatagctccc
     5161 agttttcaat agttataagc ctatttttgt ttgttgattt atttgttaga aatccatatc
     5221 tttcaaattt ctttaaagca gttcttacat tctgaatact tacatctttt gaacacatat
     5281 ttgctatact ttccaaagat gttattaact gtcctgattt aacttcatat tctttacctc
     5341 cataaaccca tttattaggc ttatgattaa ctgacagtaa taggtttatg agtatatctc
     5401 tttgtttgga attacaatct ttatatagtg ggctttcttt tatctttcta tgaagcttta
     5461 tccaacctag attactcaaa tactatcacc cacttaagtc tataatttta taccatttgc
     5521 attttcaata aaccaattat ctagtttatt ttttattata tacactctat tatctatcca
     5581 tatacaagga aatcctttat ttttagaatg acataactgt ctaaattttg attcccctat
     5641 gggataaatt ttagaagctt ctttacaagt gataagttga gctttatctc ttctatatgt
     5701 ctgtatctcc ttgtaccctt tttccttttc ttgtgcttct tgttcttgct tgacaagtat
     5761 attcaatgta gcttgtagtg ttttaagaac ttctagatta tttgtatctg ccatacatca
     5821 ttcctcctta tcaaaaaata attcttctac acttgtatct agttcttgag ccaattttaa
     5881 catcaacata ttacttggat tttttgattt acctctttct aaagaagata aataatatct
     5941 tgagatgcca actttttcag ctaattcata ttgctttata cctttttcaa ttctttttaa
     6001 tttgagtttc attgatttcc tcctttcttt catacccatt ttgtgtatcc tcatttctat
     6061 aaaatattct aacacccatt tggtgtattg tcaatatatt taagtgtatc aatagaccca
     6121 tttagtgtta taatgtattt gaggtgaaat tatgatagat aatacaattg gaaaaaagat
     6181 taaacaatat agaaaatcta taaatatgtc aagggatgag ttagctaata agattggttg
     6241 ctcagtttat gctgtagcta aatatgagca aggccaaaga aagcctagtt tagatatgat
     6301 aaaaaaaata tctactgcat tatcaattcc tataaatagg cttattattg atgaaagtga
     6361 tttagatact tcaacaaata attatactaa tttaaaagaa ttggatttag ttgtaaaaac
     6421 atttgataat tactcagcaa aactcatcgg aaataatata agcaaattta gaaatataaa
     6481 taatatatct ctcaaagaac tctcagaaaa aataaattgt tctgagaatg aaattataaa
     6541 ttatgagaat ggaacaaaaa tgcctagaat tgaaatatta aaaaaaatag cacttgctac
     6601 ttatactaag attgaagact tatttggatt tccattagaa gagataaaaa tagataacca
     6661 aacaatcctc tatagaaatc cccagactgg atatattata gataactttc ttatcgattt
     6721 tataaataaa gatattaaca tagataatac tccagtttct tattatatag ataagtcaga
     6781 atttgattta gtaattcata tattaaaaaa ttatggttat gatgtaaaaa cagattataa
     6841 gacaaagttt gaaattgcag ataatgcaga tacaataaaa ccaattagct taagcttctc
     6901 tgacttcaaa gacttctcca aaaatctaaa ttgggtgata aatggtcttg ttgaaaattt
     6961 tatacaagaa aactctaatg gtcttattga agatgaggat gaagaatatt agtattgcac
     7021 attttcaaaa tatgagttta cctattaact tttatacatt catagttaat aaataaaaat
     7081 tagatatgat atgaaaggag ctaaaatcat gaataaatca tatataccaa aatacacaca
     7141 taaattccct actaatctac ctcatggaga taaaaaatat actttaacat acattagaag
     7201 aatgataagt gaatttgtat atgatatggg taacctagaa aataacccct ttacattccc
     7261 agaagtacaa acattacttg atggtataac cgtaggtgga cataaattaa gtgaccaaaa
     7321 tcaaatacta aatataaaat catcatggga ttatgtaata aaattaagta acaaagaaaa
     7381 cttatcttta aataaagata caatttgctc tatacataaa atagttgcaa aagatgaagc
     7441 tctaatagtt ggagatttta gaaatggtaa cataggtata gctggtacta ctcaatatga
     7501 atgcatagaa gccactctat taaatgattt atttgtaagc gacataaata tcataaataa
     7561 aataactaac cccttagaaa aggctataat aataaattta tggttgtcct attgccaatt
     7621 cttctatgat ggcaacaaaa gaactgctag acttacctca aacctaatac ttataagtaa
     7681 tgatatagga gttctatcaa taccagctaa acataaacaa gaatacaaca cattaatgct
     7741 aaacttttat gaaacactag aagctgatga agttataaaa ttcttattag agaaatgtat
     7801 tacattcttt gatgaatata actataaatc atataaggag ctgtttaaaa atggcaacta
     7861 aaagagctaa tggagaaggc tctatcggaa aatataaaga tggttggcgt tcccgtataa
     7921 tgattggcta taatgaagat ggtaaaccaa taagaaaaga attttatggt aagactcaaa
     7981 aggaagttaa agataaactt gatgcttata aaaaacaata ttatttaagc tctgatattt
     8041 cagatgataa aataacttta gagcattggt tttacaattg gttatttgaa tatagaataa
     8101 aagatttaaa acctaaatca tttgaaagat atgaaagcat ctatcgtaat tatattaaag
     8161 atactgattt aggcaatgtt aaattaaagg acttaagagt atctcacata caaaaatatt
     8221 acaatagatt gcttgattcc aataaaagta tacctactat aaggcaaata aacactaaac
     8281 tgaaaacctg cttatcagaa gctgaaaaac aaggtcttat acaacgtaat tattgtacta
     8341 tggtaaatat accagttgag aaaaaggaga ataagcttga aatattgact cttgaacaac
     8401 aaaagagttt tataaaagct atagatggac ataaattaga ggtattattt ttacttgctc
     8461 ttggtactgg acttagatta ggagaattat taggacttaa atggtttgat attgatttta
     8521 aaaagtctaa tttaacagtt aaaagaaccc ttcaaagaac atattttata gataaaaccg
     8581 gaaatagaga gcttaaagta ttagagcaag agccaaagac atcaaattct tacagaactg
     8641 tacctattcc aaaagatgtt ttaaataaat tgaaagagca taaagtaaat caaaattcgg
     8701 atatattaaa ggctggagaa ttatatagaa atgataatta tgtattttgc aatgaattag
     8761 gtgcccctat tgatgataaa agacctttaa ggaatctaaa atctatatta aacagcttag
     8821 atatagagcc tataaaattt catggtctta ggaaaactta tgcgactaga ctttttgaga
     8881 atgatgtccc tcctaaaact gtacaagttt taatgggtca ttatgatata tcaataactt
     8941 taaatatata tactcaagtt atggaagata agaaaattga agctgtagaa aagttggata
     9001 aaatattttc attgtaaaaa tcaaatatat aaaaatactc tgatttcata tagttaatta
     9061 caaaaccaaa ataaggatac aaattaattt tatattatct tgtatcctta ttcttgtcta
     9121 gttttttaaa tatttatctt ggatagcctt tatgatacat taatccattt tctaacatta
     9181 gttcttgggc taaatatata tgacccttac agtgcatatc tgatataccc tcacttataa
     9241 gtttatatgt ttcagaagta tataaataat ccatactttc ttccaagctc ttacctgttt
     9301 gtttagaaaa ttctaatatg atttttttat atagcgattg taacaatgtc ttattagctt
     9361 ccataaaagc tatacctcct ctttcaaata tttgtttatc ctattttgac taactatata
     9421 tttattagtt atttaattag ccataataat tcggctataa gatatttata atctaatctc
     9481 aattatataa atctttttgc caatatttaa taatcaaaat attgcactta attaactatc
     9541 ttataagtat tgataataaa atctgtcttc ttaaatatac ttaaataaaa cttctataat
     9601 tatatcaaag tattaatatt cactctctat tgtctctcct acaagtctat tttctccatt
     9661 tagggtatat actcctgata tagttggttg tatgtatttt ctatccatat atggttcttc
     9721 taaaactctt ttttctgcta gcatcgtcca aaatacacta tatccttgtt cattaagata
     9781 ttttaagaaa atttccttat taataaagaa tcctatctct tcattaagta attcactact
     9841 ttcaaaacaa acaacttcac cataatcatt atatagaatc gaatctccct gaccatatct
     9901 caatttcaac ccataaaata gagtactaca aggtttatgc catgtgccag aaaagtcttc
     9961 ttgaaatatg tcaccttctc gttcagtagt ataactataa gttggcaata atactttata
    10021 atccttttct aagatattct tatgttcatc tatttgtacc cactcttctc cacaataata
    10081 tgaattttga aaaaagtaat atccatctgc ccaataatat tccttattat aaagagtata
    10141 attactatta ggttcagtaa gccatctacc cataaagtct tttcccttta gagcattcac
    10201 tattggttct agttggtcat ttttaattat atagctattg attaataccc acatgtcttt
    10261 ttcaggattt ttatagttct tatttttaaa atcattttcc tccttccaag tataccatcc
    10321 attaagtaat aaaaagtctt gatttttgta aacactattt accatatcat ttatgcttgg
    10381 taaatcacta aaatctccta accactcatt attaggtatt ggagaaaatt gaaataattg
    10441 attatgtact cttttattgt tattattatt tattactgtc atgacagttg gatcaatatt
    10501 cctaatatct ggctcaaatg tacccttaca ataaatttct tttttagtac cataagcatc
    10561 tgtatacact tctatcttat aattatcagc agcttgcgct gcaagttcat ataatgcaat
    10621 ccattgatat ttctttccta ttctttcata tttactatct cttaatctac cattttgtac
    10681 tgttctatca aatttaccat gtttatttac atcatatccc atatcaaaaa ctttcttaat
    10741 agctatattc tttaaatcgt tatagtctag acctttccat ttatggaaat agtgttggaa
    10801 catatatctt ccaaaatctc cataccatcc tggttgtcca tctctagtat attctacttc
    10861 catagaattt aagatagtgt tttgactcca ataataatct ttaaaatctg atgccttata
    10921 gtcatactta tatccctcaa tttcttcatc agttggtact ttaggaaaaa ttgatttata
    10981 tggtggttca acattcatat cttttaatac tgaactatca attttatatt ttgcataatc
    11041 tacaatattt tttgcataat ctcttaataa aatattggga taaacataat ctacatcaaa
    11101 tattttatta taaacataaa ctgccaatgc ctctatcatc tcttctgatt gctctgatac
    11161 aacacatcca aatgcaactg catataatcg ttcaataata tatgggtcat ctacatcttc
    11221 aaatgtttta agtaaatcta ttacaatatc cattttccct gttaatagat ttacaagagc
    11281 ttttgtactt ttatctctta agtctctatt tgaagaaatt aaaaacgttg caatcatcat
    11341 agctgacaat ctaattgttt catttgaagt attatcaaaa ccttcaaaat caaagcacca
    11401 tcctaataat cgcttaatag aagactcttc ttcttcataa acttcattaa atataggtat
    11461 aaagatagca tctctatctg ccatagtatt ttgtaaaata tattttacta tataatctgc
    11521 atttaatgta tgattaacct ttgtagaaat taagataagt acattataaa aatctatttt
    11581 ataataagat ttacctaaaa ctattttatt aatataatct agtgtttttt cagtaattgt
    11641 agatttctta cgccatttta gtgcattgat aaatgcacta ataacattat aatttgtttc
    11701 tttattttta aaaacttcaa aaatttcatt aggtatatct gagcctgtct cttcagataa
    11761 aacaattgcc aatacttcta atatatcctc atattctact aaatatttat acttatgtga
    11821 aaaatctact accccaattt cttttaactc tttaaccaac aaatttgcat ataaataatc
    11881 ttctaacttc tcatatgtca catgcatata ttcttcatca tttaaattga ctgttttatt
    11941 aactattcca tttgctatga gttcatctat tatcacattg ttaatcttat acttacttgc
    12001 aatatttatt ataatctcta cactcttgtt tgcgggtatt aaattattcc caagaccatc
    12061 tccatattta tatttaatca tttcattaag tacttctgta actacattgg catgtctact
    12121 ataattgcat gattcagata ttctaagatt aagtatattc aaataatttt tataaatatc
    12181 tgtaaaacta atagtatcta agttaatctc ttctccttta aaagtttcac acataattct
    12241 caaaaaaaga gggtttctaa attcttgatt tggcattgga atattagaat aaggtattcc
    12301 ataataatca aaatactttt tcatagcttt atgttctata ctagaaaatc cttgatgctc
    12361 tataaaaacc aaattctctt ttatcttgtt attattatct attaaagatg gtatatattc
    12421 acttcttatt gataatatta gtccaatcca tggatatttc tttatctgtt ctacaatacc
    12481 atttaaataa tatttccaaa gttcttttcc ttgaccttca ttcaatgcat ctataaataa
    12541 tatcacacgc tgttgctctt tttctcctat ttcattaagt atttctaata attcttcaaa
    12601 ctcacactca atttctaata tttctaacat atgtttaatt ggtgttttat ccaacttaaa
    12661 atgttgccct aaaaataata tagacttatg cccatccata tttcttttct caatagtatg
    12721 tgcaattata tgagatttcc ccataccacc tgcactatgt aataaaatat aagggctatt
    12781 aactactttt atttccttag aatccaaata ttttatatat acatagatat ctttttgagt
    12841 ctgcttcaat ttgtatattt catcttctct ttttttactt ttttcacagt cttcatttat
    12901 aattatattt ttaaaattat ttattctatc atcaagtgct gcacttattt tttcaaagtg
    12961 cttttttaac aacttgatat ctaatctttc attagctttg tcataaaatt gttgtattat
    13021 ttcttctata actagtacat ctacttccac ttcatttatt ttctgtttct ttatacttga
    13081 tagaacttta tctgtttttt catcaaaacg ttgtttaaat tcttcatttt ttcctaatgc
    13141 attaaacgta ttttctaaac tcaattctac attcaattct ggtaaatatc tatctccaag
    13201 gttttttatc tgtatattat tctgattttg aaaccatttc tctgttaatc tacaagataa
    13261 attagttaac ctactttcct ttaaagacct atcctttaat cttctatatt catctggcaa
    13321 aactatttcc aaaattcgtt caagtattat tcttacacac tcatgcttac cttcaggata
    13381 cataatggtt tgtatataat actcattttc taactctatt cgtctatgtt tctttttctc
    13441 actactaact ggaataatag cataatgacc cgttcctgcc tctgatactt gctgtaataa
    13501 ttccaacggt ctatcctttt ccaaactaca tccaagaaat aacaactctt tacttgtata
    13561 tacttgtgta agcatctttc tcaattcatc attactatat gctttttcat attgttcttt
    13621 tgttaacaca atagatgttg taggctctgt aatatcccca tgaattttat acaatactaa
    13681 ttcattattt ctaagtgcat tatttaatgc ttcaaagtga cctggatgtg acactaaaac
    13741 tgttttattg tgtaatccat aatttttttc tagtacttta tcataattag tagttataat
    13801 taatcctgag ccaaaaatta taggaattaa atagacacat ttatcaataa aatcgtcttt
    13861 atcttttgca tccagataac ctgccccaaa aacttttttt aaatgtttag aaaatctaac
    13921 tttcgccaat tcttcatata aaaattctgc ttttttctca taatcctcta ttttttcaaa
    13981 taaaatttta tcctcatctt ctaaatattc ttcacttgtg ttatttaaaa atgtactcca
    14041 tgaaggatat atgtctttaa ataacataga catgcctgct ccaatatatg ggactacata
    14101 accatcttta attgcattta ccagttcatc aaaatttttt tgattgtaag gcttattaaa
    14161 ttccataatt tcttcaaatg tcattattcc ctcctaaact atctcttaat aatttcaacc
    14221 ataattccta ttttataatt ttattatatc attccattcc atttttttac tacaattcta
    14281 tgttatagaa tgaattcact tacttacatt tgttaataat ataaaacttt cttttaagat
    14341 aattaaaatt tatactttta aattatggat aaaaatggaa tgttataact tttaatgtat
    14401 tactaatatt aacaatttta aaaaataata ctattcttat tttatgctat aattatattt
    14461 tgcataaata ttattgatat tttataaata taattatact tatatctcat actcaaatat
    14521 tttatataat atataagtct aaattaactt gcaatataaa agcgcctctt aatataggcg
    14581 ctataatgtt ataaattttt aattatatat tccaatgaac ctttatcaac catatctaaa
    14641 ttaaatatat aatccatctt atcaaatgca tctcttaatg gaatacttga ttcaagccat
    14701 cctaacccat ctgtaatcca acaaaatgta aaacctccat cttttatata cttatctaac
    14761 ttagtaaact catttgcaac agattttaac ttagaacctc caccattata aaaattaact
    14821 tctactaaaa caatttttcc attattattt atagcaaagt catactttct tttactctta
    14881 tctgttggaa cttctataga ccatttctca cttatttttt tcactgtagc ttgtgttaaa
    14941 taactccaat tattcttttc acacatattc tttacatatc tttctacaag agtttccata
    15001 ctacttccag ttctattttt tctagcattc gtatcaagcc caacctctac tccaaacaca
    15061 taatctacta aattttttat atttttttgt tttaataaat ctaaaaactt tattttttta
    15121 agaaattctg tagctaattc gctttcttca tcacttatac tctttctctt cttaaaatta
    15181 aacagtttat atttaaacat atcctcatct gatggatcta gtattttgaa ttcattatct
    15241 ctactcgcta ttactattgg tatcacacta agaacttctg gatactcttt taataaattt
    15301 ttagtttcgt tttctatatc ttcttttcct ataagatagt ttaagatatt taactgaacc
    15361 tctatctttc tagtattttc caaagcttta ttccagttaa cataataatc ccagctagct
    15421 atagtacctt ttaaattttc aagtaattcc ttaaatattt catccttact cttattttta
    15481 tgaatattct taaccaattc catttgattc cccacctctt cataattagt aattagcaat
    15541 tcagttataa aacctcttcc attcccctta gaatttatga atcttttagc atgtactctt
    15601 tttatattaa aatctgaata caaatcatca aagaaagtat cttcttcatc taaaactttt
    15661 ggatcagaat tacttaacat caattttgta tctattttat ctagaacttt ataaaattta
    15721 cttaactcaa tttgactatc atcattaaaa ttacctttgt tataggaatt aaatcctcct
    15781 atagttactg gtctatatgg tggatctaga tacacaaaac aatttttatc tattatattt
    15841 tctagattta aataattctt atttaatatt tttactttta gctctcctcg agaatctctt
    15901 ttatttaaaa gcatggataa tttttcaagt tcatttttat caaaaaaact aggattttta
    15961 tatcttccaa aaggtacatt aaattcacct cgagaatttt ctctatataa tccattaaaa
    16021 catgttttat ttaaaaatat aaaatatgaa gcttgcttta tacaatcttc attttctata
    16081 tttttttcat ttgtaagtat attaaatcta tctctaatat catagtacat cttttctcta
    16141 tctgaagaac cttcttcgta agatatgtat tgttctttta aattagttag ttctgttatt
    16201 aactcttcca aatcatctct gactttaata tataaattaa ttaacttact atttatatca
    16261 ttaattacat acttgttgaa aatgtcctct tctaacaaat atagaaatag tgcacctgca
    16321 cctacaaaag gttctatata agtcatggaa ctaaaatcac aacctgtagg catttgtttt
    16381 cttagaatat caagtagctg tgttttacct ccagcccatt ttaaaattgg tttcatgcaa
    16441 cacctctctc agttatattg ttatataatt atataaaata agattctaaa atacaagtac
    16501 tcatcagaac actatattta tatcaaatct ttccaaaatc taagtattat tttatcctaa
    16561 cacttttcaa aacctctaaa taatatacaa tattctctaa tatttactta ctaaaaattt
    16621 aaatctatct ttaagtataa cctaaaacag attataagtt acgtagtttt taatataaaa
    16681 tatacttcta caagtaaagc tttaaatatc aattacttat ttcataatta atgactatgt
    16741 accctcaaaa aaacctcatt ttctatggtt ggggtacggt tggtggattt catcaccaaa
    16801 ataaaaaata ctagtcaata aagactagta tttccaagta tttcatcatt cttttttcaa
    16861 aattggtgga gacgagggga gtcgaacccc tgtccgcaaa tcttttcctt gctgcatcta
    16921 catgcttagc caacttcact aatatctcac ctcacatagc gccaagtggc aggctctatg
    16981 cttagccagt cccaattaat ctacttcagg tcgagacacc cctaaagtag ttccctgcat
    17041 aatgacgtcc agtcttagtc ggcaggagga ctaagaggga cgagcagcaa ctaggctgct
    17101 atcgcgaaat tatcgtctgc gtttatttta aagttcccca gtttttacgt gacccagagt
    17161 aacacgacat gcagcctcaa gttccaaacc cacgtcgaaa cctttacgcc cccttgagta
    17221 gtttatagtc cagtagactt ttcgcaaact ttatcaccat ttttattaaa tttttaatgt
    17281 atatataata taatactttt aagaataaaa atcaatgtta tgttctagat aataaagaat
    17341 tttattctaa aaaatacagt tttatctagt ttaatactta cctgtcattt ctctttctat
    17401 acgtctttgt gcatctttct tagccatatc ttgacgttta tcatatagtt ttttaccttt
    17461 tgctaaggca atctctactt taactttacc gttttttaga tacaaactaa gtggtataag
    17521 tgaatacccc tttatagtag ttgctccaat aagctttctt atttcatgct tatgaagtaa
    17581 tagttttcta actcttaaag aatcaacatt gaatatattt ccttgctcat atggacttat
    17641 atgaacttgt tttaaaaata cttcgctatt atctacagat gcaaatccat cacttaggtt
    17701 aactttgcct tgtcttattg attttacttc tgttcctttt aattcaattc cacattcata
    17761 agtttcttct ataaaatatt catgtcttgc tcttttgttt gttgctaata tttttttacc
    17821 agccatataa atctcacctt ctttgctaga aatttattcc ataaatatta gaaatttact
    17881 aaatatttaa tgttgccaaa ttatttgact aacttgtatg ataacatatt taggcatttt
    17941 agtcaatatt tcactgatat attaagttag ttaggctata tataattaag taatataaat
    18001 gtatgaaaat agctaaaaaa ctacttcgaa tatacttaat actaaactaa ctctaaccag
    18061 taatactaat ttataaattt caaaacaaaa atattacata ttataaatct tctatcttct
    18121 atcttctatc ttctatcttc tatcttctat cttctatctt ctatcttcta tcttctatct
    18181 tctatcttct aaataatttt aacatgccca taaaatataa tacccacaaa tgataaataa
    18241 tactcaaaat gtctattgaa ataactatac atccttaaat aacttaactt aaatctaaac
    18301 atttcaaaaa cttcttaaaa atatgtaaaa atttgatgat aaagtagcga ttttttcaaa
    18361 aaaaatgtaa tatagagtta tatcttataa tattttagag ataaatttta tcatagtggg
    18421 gagaaatttg tatggattta agcaaaaaag taaaaatttt agaaaatgca tcagatggca
    18481 aaagcgaatt tgagatatta gaatttgaaa aacttgaggg agcttctagc ccattaatgg
    18541 caatgggatt atattttgct agagaagctg gtttgaaagc tagacaaatt aagataaaat
    18601 taaataattc atcaataaaa acagaagctg gtgcattata ctattataaa ggaaatatat
    18661 cttctgaaac taagattggt ggtgttggag gtctttttaa aaaaggaata tcaggagcac
    18721 ttactaatga atcaatgatg aaacctgtat acaaaggtac tggagaagtt tatttggaac
    18781 cttcttttag acattattta tttatgagtc ttgataatga ttctataatt gttgataaag
    18841 gattattcta ttgttgctct gatacaatag aagtaaaagc atttacacaa aagaacttct
    18901 catctgcttt acttggtggt gaaggcttct tccaaataga attaagtggt actggtgtaa
    18961 tcgttctgga atgtgttgta cctcaaagtg aaattgtaga gtatgaattg aaaaatggtg
    19021 aagaattaaa ggtagatggt aactttgcaa tagcaagaac atctggtgtt aatttttctg
    19081 ttacaaaatc agataaatct ttaattggtt ctgctttaaa tggtgaaggt ttcttaaata
    19141 catttactgg taatggtaca gtttggctag ctccaactca agtaatgtat gaaaaactaa
    19201 gttttggagc aatgcctact aatgataata tgaataataa agtttctaag caaaaatagt
    19261 tgttttataa atatttatta aatcattatt gattttataa agtcactata aattttatta
    19321 gattattatt ataaaatcag agtgtggaaa tgtttatgtg tattctttaa ataaaaattt
    19381 ccacattctg taaggttatt atacttgata tataaaattc aatttaggaa catagtctaa
    19441 catttataaa catattaagg taatgatttc tcttggtaat tattctcata tcttattctt
    19501 tgattatatt tatcttattc ctagccctta tgtcatatta tatttcttat cttattactt
    19561 ataattagca ttaacttatc taaaatcttg tattttttta atagtaaagt ttacatctat
    19621 attaaaaagt ttacatctat attaatttca taaaacaatc aaggtagtca agagattcaa
    19681 ctttacttga ttttaaaaat ttaagtgaat ttgttcttat acacatcttt ttaaaattat
    19741 atctgtagaa aatatcattt agagctataa ttaaattaac ttattaaatt ttagaaaatt
    19801 tattaaaaaa tataagttaa attaatattt ttaaagattc ttttttgtaa ctactagaat
    19861 agtaatttag tagttattgt atctctaaag tttaagcatg gatagatata catttgataa
    19921 tttgcctata atttacctac tacttggatt attggagcaa atacaatata ttaataataa
    19981 gttcctaaaa ccaataaatt aactaataca cataaagata ttgatgttga ttatacaact
    20041 ataagaaacg gatttaacag aatttaatca agctaagaaa ttatatatat atcgtgatgt
    20101 ttttgaatta gaaggtaatt caaaattagt ttatagtctt ctgtcaatcc aataaaaagt
    20161 acagggtaca agaattgtta gtaaaatttt taaagatttt atggataaat ataaatagtt
    20221 ttacttattt tctaaaactt taaaatacca ttaattacct taaatatttt aataggagga
    20281 catatgaatt taaataaagt taaatttact gaagaacatg ctattgaagt tactaattgg
    20341 aaatatgaag gtaaatattc aatatacaat ctacctccat gggaggaaat taaaaagaag
    20401 aatttttcac tagcaaaaga agataaaaga aaaaatttta taagttttat aaatgacaat
    20461 aaagaattga taggttttat aaacttatta gatgaaggaa gctctgtatt ctttggtatt
    20521 ggtatgaaac cgaattattg tggaatggga attggtaaag aaataattgg cctaggatta
    20581 caagaatgta gaaataaata tagtactaaa ccaattgtat taaatgtaag aacctggaac
    20641 atgagagctg taaaatgtta tgagtctcaa ggatttaaaa tagttgagac taaggtacaa
    20701 aaaacgcatt taggagatgg agaatttttt gttatgaggt atcaatagtt tataacaata
    20761 gtcttattgt taatacaaca attaaaatga aaaaatacat atgtgtaggt catagcaata
    20821 agtgtctttg aaatataaat ttgagagctt ttgataaaat tttgtataca tatatatttt
    20881 atactacgga tatataaatc tatctacata tataaagaga ttgtaagtag gtttgtgtat
    20941 ataaaaatag atttatattt cataaatgta ctttttattt gtattacctt tattttatct
    21001 aagctctcat tttattatct aatcttcctt tttactggtt ttattaagag agatgcaatt
    21061 atcaacacaa cacttccaag tatcagccaa tatttctcat caaaactacc ttgactcata
    21121 gacattataa aaaattttcc aacatacttg cctttagtca tcatatttaa aattaaatat
    21181 cctatagata tcatataacc tatgattgta ttattaaata aatttgagaa aaacattcct
    21241 aaacttccta taaacatagc tgatgcgaaa cttccaataa tatactcaac tactggaaaa
    21301 ttacatccaa gatatattaa aacatttaca aaacctatga ttaataccac tatacctata
    21361 attgacatca gtatcctaat taattgtaca ataacttgag ataatgcttt tgaagatact
    21421 atctcatcaa tttcttttga ttgctctagc gaaaatacag gtaccaacat tactatccct
    21481 attattgcaa cataattttc taaacatata gctgttgaaa ttctgtctaa attagctact
    21541 ccttgtatta gaggaaccat acatagcaat ataattgaac atattaaatt agttaaagtg
    21601 cttaatttga agttttcttt tattagaact aaacagtttt gaaaactttg cccaaacatc
    21661 aagattcccc cttctttttt gctcatataa gaatatagct aacaaaacaa gcaccaatgc
    21721 tcccaagctt atcacaattc tattaatcat aatttgattg aagttttgta taataatatc
    21781 cccatttaat acactattat gtcttatcat tagccccatt gttgaataat cacctttgag
    21841 tcctgtagaa tttatactgt aaagccacca aattaattgc acaataattg ctactggtgt
    21901 atctgttaat atagtaatac tcattcctac agctgtaact atcataagag ttggtagtat
    21961 ccaccataat gtatatttta taaatgctaa gttatcaata gatatacctt tatctttagc
    22021 aaagaaaatc aaaggaagta aatctttcat acctaaaatt aatacaggca atatcatcat
    22081 aaagacaagt gcaaaatatc ttgataaaac tattttgcat gaagatactt gttttgaata
    22141 aataatctct ctcattcttg aacgcttatc tttaagtgac ataaaaactg ccacaaatac
    22201 tggaaatata cttaaaacta tacctatata atcgcaaaaa attcttgcat acccattact
    22261 aactttatct ttttgtattg tatagttgta attcttcata gcttcatcat aggtaactgg
    22321 aactactcca aatctttgta acttgtcaaa agcatatgca gaccctcctc ctaaaatgtc
    22381 gtctactctt tgcattattt cttcaaatct ttcaagagat actttagaat aatctatttt
    22441 tatactttca tcagttttcc aaagtgaatt tatttcgcta atacttttac cagttatttc
    22501 ttctatatat ttttctatgg tcttcgtttt attttggtct aattttatat acttaactac
    22561 ccctgttgga tacgttgtat atgtgttttt ttgatattct ctaattaggt ttatagttgc
    22621 atttggaatg accttttctg ttttcataga ttctttttga ccatatggat attgattgtg
    22681 tacagaataa tattcctcaa tattctctac tttttgtgga gcaactaatg gatttccatc
    22741 attaggaata tttttactta catcatttga aactgaactt ccaagttgag aaatatagaa
    22801 aaaaactaaa actacaataa aaatataata taccatactc tttaatatat ttttacattc
    22861 ttttataaat agattcatat attatctcct cccattataa gcatatagcc atcttcaata
    22921 gttggattac aatttactgc tgtctcaaaa ggtttatcat ctgatataat cctaagtata
    22981 gcctttccac catgagtcaa cattcctata actataaatc tactttttat attttcaatg
    23041 tcttttttat ctacttcaat actatatact ttcccttcag ctagttttgc taatgcttct
    23101 gttgagccat tgtatatgac ttttccacta ttcaatatag ctacgttttc acatgtagct
    23161 tctatatctc caacaatatg tgtagataaa ataacgattc tatcatttgc aatttcgcat
    23221 agtaaatttc taaatcttat acgttcttct gggtcaagac ctgctgtcgg ctcatctact
    23281 attaaaactc tgggattatg aattaatgct tgtgctatcc caagccttct attcatacct
    23341 ccagataaag attttacttt cacattttga taattagtaa ggtttacttt gcttaataac
    23401 tttgctataa tttttcttct ttttcttagt ggtatatccg ataaaactgc taaataatcc
    23461 attgctttat atactgtcat gttaggatac attgaaaaat cttgtggcaa ataaccaacc
    23521 atatttctaa tttccttagt attctcaata ggaactccac acatatgtat tgaaccttct
    23581 gtttttgata ataaagtggc taaaattctc ataaatgtgg tttttcctgc tccatttctt
    23641 cctaaaagac caaacatacc actgtcgatt tcaagattta tattatcaag agcttgtttt
    23701 ttcccaaatc ttttactaag attggatact gctattttca tttccatata taaatacacc
    23761 cccagaaac
//
LOCUS       DAELTA010000046        23357 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.046, whole genome shotgun sequence.
ACCESSION   DAELTA010000046 DAELTA010000000
VERSION     DAELTA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.046"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            183..1415
                     /locus_tag="KPG13_001583"
     CDS             183..1415
                     /locus_tag="KPG13_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0985316.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1429..2235
                     /locus_tag="KPG13_001584"
     CDS             1429..2235
                     /locus_tag="KPG13_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG0985317.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2347..3216)
                     /locus_tag="KPG13_001585"
     CDS             complement(2347..3216)
                     /locus_tag="KPG13_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0985318.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3588..5015
                     /locus_tag="KPG13_001586"
     CDS             3588..5015
                     /locus_tag="KPG13_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG0985319.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5092..5361)
                     /locus_tag="KPG13_001587"
     CDS             complement(5092..5361)
                     /locus_tag="KPG13_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985320.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5364..6806)
                     /gene="nhaC"
                     /locus_tag="KPG13_001588"
     CDS             complement(5364..6806)
                     /gene="nhaC"
                     /locus_tag="KPG13_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG0985321.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6831..7997)
                     /locus_tag="KPG13_001589"
     CDS             complement(6831..7997)
                     /locus_tag="KPG13_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0985322.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8222..10342)
                     /locus_tag="KPG13_001590"
     CDS             complement(8222..10342)
                     /locus_tag="KPG13_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985323.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10575..10976
                     /locus_tag="KPG13_001591"
     CDS             10575..10976
                     /locus_tag="KPG13_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985324.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11019..11312
                     /locus_tag="KPG13_001592"
     CDS             11019..11312
                     /locus_tag="KPG13_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG0985325.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11327..11767
                     /locus_tag="KPG13_001593"
     CDS             11327..11767
                     /locus_tag="KPG13_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG0985326.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11859..12332)
                     /locus_tag="KPG13_001594"
     CDS             complement(11859..12332)
                     /locus_tag="KPG13_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0985327.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12332..13120)
                     /locus_tag="KPG13_001595"
     CDS             complement(12332..13120)
                     /locus_tag="KPG13_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG0985328.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13448..14692
                     /locus_tag="KPG13_001596"
     CDS             13448..14692
                     /locus_tag="KPG13_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0985329.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15015..16112
                     /locus_tag="KPG13_001597"
     CDS             15015..16112
                     /locus_tag="KPG13_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG0985330.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16103..16801
                     /locus_tag="KPG13_001598"
     CDS             16103..16801
                     /locus_tag="KPG13_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0985331.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16804..17868
                     /locus_tag="KPG13_001599"
     CDS             16804..17868
                     /locus_tag="KPG13_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0985332.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18051..18755
                     /locus_tag="KPG13_001600"
     CDS             18051..18755
                     /locus_tag="KPG13_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985333.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18748..20070
                     /locus_tag="KPG13_001601"
     CDS             18748..20070
                     /locus_tag="KPG13_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985334.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20257..21630
                     /locus_tag="KPG13_001602"
     CDS             20257..21630
                     /locus_tag="KPG13_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985335.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22002..22940)
                     /gene="pdaA"
                     /locus_tag="KPG13_001603"
     CDS             complement(22002..22940)
                     /gene="pdaA"
                     /locus_tag="KPG13_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG0985336.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8606 a         2909 c         3699 g         8143 t
ORIGIN      
        1 aataataaat ttggactatt tatctattta taacttaaat tatatcaaat tgataacaaa
       61 ctagatatta tacttttttg tagaatagaa ttacaattat ataaagaaaa attctattaa
      121 tagtgaaaaa aaagtagaaa aaagtagaaa ttattctttt ataaagaaag ggagggataa
      181 tattgcaaga gagagaagaa ttagttttaa atccagttcc aaaagaggaa agagttggat
      241 ggttagctcc attgtttaat atgcttggtt ctaacatagc tatatctgaa ctaatggttg
      301 ggggaacttt aattttagga atgactcttt ctaatatgat tattacatca attataggga
      361 acttaatatt agttgcaatt attatgatac aaggttatat aggttacaaa gaagggttaa
      421 atacgtatgt attagctaag ggggcatttg gagagatagg aggaaagtat cttatatctc
      481 tattacttgg tataactagt tttggatggt ttggtgtaca agcaggggtt gcaggactat
      541 cagtacaaaa gatattccca agtgtaaacc taacattagt tacagttata cttggattat
      601 ttatggttgt ttttgcactg tatggtttta aagctatggc taaatttaat tacttagtta
      661 taccaccact tatgatatta atggtgtggg gagtttttaa agctttttct acttatggag
      721 ttgaagctat ttataattat actcctcaaa caactatgag tatggttgaa ggtcttaata
      781 ttgtagtagg tcttgttata gttggtgcaa ttatatcacc tgaccagtta agatatacga
      841 gacgagtaaa agacatttgg attatcagtt ttttaggtct agggattatt tcattattcc
      901 aacaagttgc agctggtgta atgtctatgg gagcacctac ttgggatata acagaagtat
      961 tggctaattt aggttttgga tgggtagcgt ttgttatttt aatacttgca tcttggtcta
     1021 caaacgtatc aaatgcttat tctggtggat tagcattaaa aactattttt ccaaatgtaa
     1081 aaagaaatgt tttgacttta gttgcaggtc taataggtac aataatagca gctactggta
     1141 taatatttaa atttcagtca tttttatctt tcttgggaat cgcagttcct gcaatagcag
     1201 gaattatgtg gtgtgagtat tattttatac aaggaagaac ttataagcat agagaaggaa
     1261 taaattggat tgcagtaata agttggttaa taggttttgc agcgtcgtat tatagttcaa
     1321 aaataaattt cttaatacct ccgataaatg gaattgtagt gagtatggtt atttattata
     1381 tattaatgaa ttgttttgga atcaaagata aataaagggg gcaattacat gggtcgtaag
     1441 gtgaaaatag gtataattca acaacacagt gtacttggaa atgttaaaaa aaatatagaa
     1501 aaagcagtag agatgataga tgacttagga aaacaagggg cagatataat ttgtttacca
     1561 gaactatttg ctacaggata taatttagag tctttaggtg gagttaaaac attagaatta
     1621 ataagagaac ataataagta catagaggaa tcaatgtcag aagctgctaa aagaaataat
     1681 gtatacctga tatctccata tggaacctta gaaaaagggt ctacacatgt ttataattca
     1741 gcagttatat ttgatagaaa aggtaagatt atgggagaat actgtaaaaa tcatctgtgg
     1801 tcattagaag cagtatactt taaaggaggg gaaaaagtag aggtatatga tgctgatttt
     1861 ggtagatttg gtgtaatgat atgttatgat gcaggttttc cagaagtatc cagagaattg
     1921 acattaaaag gttctgaaat aatatttata ccatctgcat ggagaataca agatgaagat
     1981 atgtgggatt taaatgtatc tcaaagagct ttagagaaca ctgtatatac agtaggtgta
     2041 aatttagtaa gtaatgactc taatttaata ctatttggaa agagtaaaat atgcaatcct
     2101 agaggtactg taataactca attagataca tacagggaaa aatatgtatt atcagagatt
     2161 gatttagatg aaataaaaaa gtatagggaa gatatcccct atttgaaaga cagaaaaagt
     2221 agctataaca tataattcta tactataata caagttcaat ataaaatgat aaaaagagga
     2281 tgtatcaata ttaagattaa atttattata ctttgatata tcctctttaa tattatcaac
     2341 tttgatttaa atatttttaa aatagttgat aagaaagttt ttgaacaaca acacagcttt
     2401 agattcatag ttttcgctaa accatgaaag atttaaatat cttttaaatt caatatcttt
     2461 gatatgtaac aacttaatct cagggtcttg agataatccc catgattttc cacatataaa
     2521 tccaactcct tgaagtgatt ttataagagc ataaattgta tatggtgaat cagactcaaa
     2581 tgctattttg ggtttgaaat tagctgattc gcacaggata tcaattacct ctctataatt
     2641 ctcacccttc gttataacta taaagttttc atctgaaact tcgcttagat atacttcatc
     2701 ctttaatgat aaaggatggt ttattgaaac tcctaatagt atttcttcgc atgttaaagt
     2761 tatgctattt tctgagttta gctttgtaaa tgaagaagat atgtacaaat caaaatcact
     2821 ttttttataa gatgatggca aagtatgtga cacattaaaa gtaatattgg gatatagttt
     2881 tctgaaatta ctcaaaagtg atggtaacac attagccgct gcaggagata atatctttac
     2941 ttctcctgaa ttttccaaag aagtgtcttg taactccttt tttgaacttt ctactaaatc
     3001 taaaatgcta tctactcttt ttagaaacaa tcttccatac tcatttaaaa caatatatct
     3061 tccttttctg tcaaataact gaactccaag ttctttttct aaggaagata tagttttgct
     3121 taaagctggc tgtgctatat ttaacttgtg tgatgctttt gttatgtgct ccattctagc
     3181 aacagtttga aaatacttaa gatgcaataa atccatagta taaaatacac ctcttttaat
     3241 atgacttgag ttatatcaat tttataatca actccacatt atacattata atataaagtt
     3301 tatataataa actacaatat attaaaaagt tatttaaatt gttatttata ttttgtacat
     3361 aatctaatta aaaaaagtaa ctttaaatgg ttaactaagg aaatattgtt acaaacttta
     3421 gttatataaa aaattcgttt gatgagcaaa gtattaatgt caagtaagaa taaaagctta
     3481 ttagataaga aaaataatat tttataataa tgatgttact taaattatta tgttatacta
     3541 ttttaaatag gaaacattta gatactataa ttttaggggg agtcaagatg aaattaacta
     3601 aatcaatatc atcaatgctg tgcttatctc tagttttaag cttatcacca gtagcatatg
     3661 caaatgaaat aagtagaaca acagttgaga atactttagt aggtcaggat agatatcaaa
     3721 ctgctgttga agtaagtaaa aaaggatgga gtagtgcaaa tgaggctgta gttgtaaata
     3781 gtgaatctat tgtagatgca ttagctgtaa caccatttgc taaattaaaa aatgctccta
     3841 tattattgac agaaaaaaat aatctaaatt cacaaactga gaaagaatta aaaagattag
     3901 gagttaaaaa agtttatata attggtctat ctagttctgt atcaaatgat gtgcaaaaac
     3961 agttagaatc taataattta actactgata gagttggtgg tagtgataga taccaaacag
     4021 ctttgagtat agctaaaaaa atagaaggat taaaagatat atcagaaata gctgtagtaa
     4081 atgggtacac aggtcttgca gatgcagtta gtatagcttc tgtagctgct actaatggaa
     4141 tggttatact tccagtatct gatgcatctg gtatatctag ctttaaagat tttatagaat
     4201 ctaagaatat aaataaatca tacataatag gctctactaa tgctgtatca gataaaataa
     4261 aacaaagttt acctaattca gaaagaatag gtggagcaga tagaaatgaa actaatggta
     4321 aagtaataga aaagttttat acatctaata aattaaacaa tgtatttgtg gctaaggatg
     4381 gaatgaaaaa tcaaaatcaa cttatagacg ctttagcagt aggagtacta gcatcaaaag
     4441 aaaattctcc tgtactaatc gtaggagaac aattaagtgc aacacaaaag agtgtatttt
     4501 cgaataaaaa tgctacctct ataactcaag ttggtagtgg aggaaatgaa aatgcattta
     4561 aagaattaaa agatttacag gcaaatagta acaataagag agtaatgtat gtaactaata
     4621 cagataaggt aaatataaga aatgatgcta caatagaagc ttctgttata ggatatctaa
     4681 ataatggtga tgaagtagaa gttttagatg tgttaaaaac tggatgggta gaaataaaat
     4741 acaatgaagg tattggatat gttagtgggt cttatttgac aaataataag cctgataata
     4801 gtaatgaaaa tataaaaata aagtatgtaa aagaaaaaga tggtctaaat gttaggaaag
     4861 gaccaagtac agaagatgaa aaaataggtc acctttcata tggaagcaaa gtagaaacaa
     4921 tagaaatgtt tgctactgga tgggtaaaaa taaagtataa tggtggatat ggttatgtaa
     4981 gtaatgatta cttatctgat acaccagtta tttaattata agtaacaata aaaaaactgc
     5041 tttaatttgt aaagcagttt ttttataaaa atttaagtct aataactatt tctattttga
     5101 taccttaatc ctgggattta ttattatgga tattatcaac atacaaacac tccaaatcca
     5161 gtgtctaatt ccaaacttag atattaataa attcagtcct gctatggtaa ataagcagta
     5221 tttaattaca attaaaagtg tatttataga aagccccttc atagaaagta tcctaaatcc
     5281 aataatagac attctaagtc ctataaatgc caataatata cctgcaatat cctgaattaa
     5341 gtaatagtat tcaaatttca tattcaatca actcttctct gagcagtatc aaatatttct
     5401 aactcttcaa tttcatcatc tgtgtatgta agcatagtaa aaccagttac accataaata
     5461 aatgaaatta taggtgtaat ccaacttaag aaacaatatg gaataaattc tagtgggctt
     5521 acaccaagag tctgagcaca gaaaacagca tttgaattcc atggtattaa tggaccacca
     5581 agtgtagctg tatcttctat aattctagat agattttgtg gttttaattt aaattctttg
     5641 tatattggag acattagagt tccgacaaat acagaagtga acatcattga tgaactaaat
     5701 gcatttgcaa agtaacttac tataaaagta gacccaacca atttagttct tgattttcct
     5761 attttagtta taagaggttg aagcagtact tctaagaatc cagttttttg gagaatacca
     5821 gcaataacga aaactacaaa tactagtaaa acggtttcag ccatactcat tattccacca
     5881 cgatttaata gtttgtcggc atagacaaat ccagtatcta tagtaaatcc tgatagcatg
     5941 taatttaaaa catcacctat tttttctcct tcttgaaata ctgctataat aagtcccatg
     6001 atagctgaac ttagtataga tataatgggt ggcttttgta gcaataaaag taataataaa
     6061 aatattattg gaatcattgc tactaaacca atatgaaagt ttgaatttaa tacatccttg
     6121 atttgattta tttgaacata gtctatagta ttatttgaat acttaaatcc aattactgta
     6181 tacagaacta tacatattac gtatgcaggt cctgtggtat aaaacatatg cttcatatgt
     6241 gtaattacat ctgtcttaca aactgctgca gctaagtttg tagaatctga tagaggtgac
     6301 atcttatctc caaagaaagc accagaaatg actgcaccag ctgttaaacc aagaggaata
     6361 cccatactag taccaatact catcattgct ataccagaag ttccaagagt cccccaagat
     6421 gtaccagtag caacagaagt taatgaacat acgattaatg ttgtaagcag aaaatattta
     6481 ggggttatta ttgttagtcc actatatact actgttggta ttgtaccagc agctatccaa
     6541 gctgctatta gtacaccaac agaaagtata attaagtttg attgaaaggc atctttacca
     6601 acttcaaatc caaaagcttc tatttcatca ttagtataac caagtttcat agctgcagga
     6661 actactataa gccatgaaag taaaaacatt gtagttatag aagcattaaa tacaactata
     6721 ccaactgatg ttagagctat tattgcaagc attactaaca aggaatatct aaatgaaggt
     6781 atttttcttt ctgtattcat tttcatagtg caaattctcc ttaaatattt ttagttaatt
     6841 gcatttttta ttcttataag agcttcttca agcatacttc ttggacaagc taagtttatt
     6901 ctttgatacc cacttcctcc aatgccaaag ctgtttcctt gatttagagc aactttacct
     6961 ttttgtacaa gtatactttc tagttcctca tcacttaatc caagtgcact aaagtctacc
     7021 cataataaat aagtaccttc tggttttcta actttaagtt ttggcatatt ttcatttata
     7081 tattttatag caaaatctat attagattct aaatattcta aaaatgattc tagccaagat
     7141 tcaccattat tatatgaagc ttcagttgca actaagctaa agcaattatt tctctttata
     7201 tcaattctgg taaaggcatc atctaacaat ttatagtctt tttcatcagg tagtattaca
     7261 tatgatgatt gaagaccagc tatattaaaa gttttagttg gagccataca agtaattgta
     7321 tttttttcaa attctttaga aatagatgcc ataggaatat gcttatgttt ttttaatata
     7381 atgtcactat gtatttcatc agatatgatt tttacattat gcttaagaca aatatctcct
     7441 agttttttta attcatcttt tgtccatact ctaccaactg gattatgagg attacaaagt
     7501 atgaataatt ttacatcttt aatcttgttt tcaatgtctt cataatccat tatgtagttt
     7561 ccattttcta gtttttgtaa tgggcttata attagttctc tattgttatt ttttacaaca
     7621 ctattaaaag ggctgtaaac tggttcttgt atcattattt tatcatttgc ttttgtgagt
     7681 tcatttatta aaagacttat agctgggata acacctggac tataaattaa ccactcactt
     7741 tttattttcc agttatgtct cctatctagc cagtttacta tagattcatt gtaagaatct
     7801 ggtcttgttg tgtagccata aatctcttgt tctagtctat tttttaagga atcaattata
     7861 caaggtgcag ccttaaaatc catgtcagcc acccacatag gaagtaaatc attagttcca
     7921 tacttttttt ccatttctga ccatttagat gaaaaattat tgcttctatc aacaatctca
     7981 ttaaaattat aattcattgt aaatacctcc agtaatttta attttatgtt aataatatta
     8041 agcaaacaac gtgccaatat tgataataaa aaatatttat tcacaagtta atatttaata
     8101 ttaagtaatt tcagagcata gataagataa aaatattaat tatacttaaa actttgtgga
     8161 aaaggttatt tctaataaat ttttaaatat atgaaaatgt aattaaatag gttaaatagg
     8221 tttaactgtt taacttgtta gacctattta tattttttag aaattctact ccttttgatg
     8281 ttataattga acctccacga ccaacaagta tgtctacaag cccaaagttt tgtagttcta
     8341 aaagcatatt tctaatctgt tgttctgtca aaaatctatt ttcctctaaa gctattttac
     8401 ataagctttt tctacctatt ctttctttta aatcataagc cttttttaaa ttatccaata
     8461 taaatatgta ttcatctagt gaccttttaa aattatagtc gtatttcatg atgtttttgt
     8521 ctttgccaat gttgaattga tactttttta tattatcact tactttatta caaacggttc
     8581 tactataatt tgaatctata gtatctaata tatattcagg taaatcacat atttctataa
     8641 tatctttttc caagtaacag aagtattctc ctaagtttct taattctctt acatttcctt
     8701 cccatctata cattttaaag atttctttta cttcctcact tagaataaaa ttacatggaa
     8761 tatcatattt taatgaacca aaaatttcaa atatatcagc aggtctttcc ctaagtggat
     8821 gtattttaag gggaagaaca ttcaacctaa aatataaatc tcttctaaac atatttttag
     8881 atacaagttc ttttaaattt ctattagtag ctgcaattat tcttacatca atgtcaatga
     8941 cattattaga accaatacgt cgtacttgtt tttcttgtat gactctaagt aatttagatt
     9001 gagaatttaa atccatttct ccaatttcgt ctaaaaatat agttccattg ttggctaatt
     9061 caaataaacc aattttccca ccttttttag caccagtaaa agccccttca tcatagccaa
     9121 ataattcact ttgcagtaga ttttcttgaa aagtactaca atttactgcg acaaaaggac
     9181 catcttttct cctagaagca ctatgaatgg attgagcaaa taattcttta ccagtaccac
     9241 tttcaccaat tataaggata gatgaatttg attgagccat tttattagca attttttttg
     9301 tattaatagt ttgtatacta gaacctaata tgtcatcaaa tgtgtattta ctgatattac
     9361 cagagtttac tagttgtgct cttaatttag cttgcttttt ttctaattga gaaaactttg
     9421 tcatctttag tataaatcca tcaaatacat ttaattgtat atgtttaatc tctaaattta
     9481 tatcaatatg atttatttta atcaatttct caaaaaatgg agttttattg gtcaatatgc
     9541 tttgaaaatt gatgtctttt atgcaatcac caacaaatga gtcaatcatt tcatttgcat
     9601 ttatagacaa aatttttcta gccatatgat tatagaattg aattattcca tcgttatttg
     9661 tgcatataat tccatcatca attatagata gcaacaaatc aaattgtctt tcaaacttat
     9721 ttgcatctaa caataactca cctgtactat aacttgtagg aattattttt tctacgtatt
     9781 tttttattag gtcatctttt attagatgtt ttaatttggt ttttgtagca atttcaacaa
     9841 tacaacttaa atctattatt ctgtatttta aatctataac ttcacaattt ttaaacttag
     9901 gcaagatttt ttcgccagga gttaggatta cagcatctgt gggagtaaac tcaatctctg
     9961 gatagcaggg caggatattt atattgtcta ttcctaatct atgtattaaa gaaattgttt
    10021 ctattgccat atctttactt aagttatata cataaataat tttatctttt tctaatttgg
    10081 atatcatatc aatactgttc ttttcaaatg tcaacttagg aacaatgatt tgagcatcat
    10141 agtgagataa ttttactatt tcatcatatt ttaaataagc agataggagt ataagttttt
    10201 ctttaaaaaa ctttaaattt ccctcttcaa atgagtataa atttatcttt agattatcac
    10261 ctaagaattg ccttatttga ttttcataaa tttctccagc gtcttttttt aaacttacaa
    10321 tagctaattc catttgtttc attttaacct ctcatacaaa tttgacttat tttagattac
    10381 ataataatta aattagatta aataatatta tttaattatt attataaatt tattatagac
    10441 tacacattga agtaaaagaa gtagctaaaa ttattccttt tatttttgtt ttttgtatct
    10501 atttggtata atagtattag agcgtataat aatttaggca aaatttttta aggaagacta
    10561 gaggagaata taaaatgaaa aaatgtcttt actgtaatag ggatataaag tcaccaaata
    10621 ataaattagc tataaaatat gatgacaata tgaatatata tccatgtcgt gcagaatgta
    10681 aggaaaaaat tgaagaatat atagcaaaaa aacctacata taagtttata aatatattag
    10741 aggttctttt aggtgtgggc ggaatattct gctttttgag taaatggact attgcagccc
    10801 aagttgtact tggaattgat gcactggtta tatttttatt cccattggca ggatttaaaa
    10861 tgaatggaaa gttaagtatg gaacaaacta agaatcaaag taggtctatt gcaatatcaa
    10921 ttttggcatt tatagtaata ataactgtat tatattttaa acaagttggt aagtagagca
    10981 taattgtata agacatcatt agagtatgag gtgagtttat ggaagaaaga aaattagcac
    11041 caaacaatga gagggaagct ctcataaaaa gattaaaaag aatagaaggt caagttaagg
    11101 gtatacaaaa gatggtggaa caagaacgtt attgtgttga tatattggtt caaatatctg
    11161 ctataaggtc tgctataaat agagtaggca caattatctt agagaatcat ataaaaggat
    11221 gtgtagcaga aagtattaaa catgatgatg ctgataaaac tgaagagatg ataaaagagc
    11281 ttatggatac tataaataaa tttacaaaat aaggaggcgg ttagttatga atcaacaagg
    11341 atttattatg gaaattgttg atgatagaac tgcaaagtta aaaatgcaaa gacattcagc
    11401 ttgtgctgct tgtggaaaat gtgctactac aaattcagca gagtcaaaag aaatattagt
    11461 agaagtagac aacacaattg gtgcaaaagt tggtgaccat gtagaagtga gtatggataa
    11521 tatgaatgta ttaaaagcag ctgtaatggc ttatataata cctcttatag cacttcttgt
    11581 aggaacaata gggacttatt atatattggg ggcaattgga atgacaactg gagttgaagc
    11641 cataagtgga ttagttggaa tttgtcttac aataatctgt tatttatact taaagaagaa
    11701 tgacaaaaaa tttagagaca gtagagaatt tataccagta ataacaagaa tattaattga
    11761 cctttaagat aattataatt aaaaaatagc aatgtacttt ttaaataaat actcaatacg
    11821 tatttgtaga gtttaagtac attgctattt ttatattttt attttgattt tctgtaagga
    11881 ataaagtttt ttctgatttt taaaagattc aatgttctta caaaagaagt tacaactaat
    11941 acaccaagtg ctattgcagc acttagaaat agcgcatctg taccttttga catagccaaa
    12001 gaataatttt tctgaatcaa agacaacatt gtattatata gcccaattcc tggtattaga
    12061 ggaagaatac ctggaattac gaacacaata gctggttgct ttagttttct tgctaaagtc
    12121 tcactgcacg ctgagaccaa agcagcagcg ataaaacctg caaaaactgc atttgtagta
    12181 aaattaaata aataaacata tacaatccaa ccaattccgc cagttactcc agctggaaga
    12241 agtagatata gtggagcatt aaagaaaaca gcaaatccag cagttgcaac acctgaaaat
    12301 aaaaaatgca aaatcataga catatcagtc attagaaagg acctcctatt ctataccatg
    12361 tatcaaggac aaaaccaact ccagaagcaa ttgcaataat aatcatagca gcatcaaaag
    12421 cacgagaaat tcctgatatt aaattccctg atattaaatc tctaatagct tttataaaag
    12481 caactcctgg gagtagtggc attatagcgc cgactataag cattttaggg tcatctaaaa
    12541 tgcctatttc aactagtaga actcctgaaa gtgcaattaa aaaagaagat actaaacagg
    12601 aaaatgcaga tattgcactt atcttcatcg ttttatcata agtaattgca gcaaagattg
    12661 ctattataaa cgtaagtata aaatttaata cataattccc tccaagcaaa caagcaaaac
    12721 aagcagatgc aattcctgta caagagtaaa ataaccataa aggataaggt tttacatctt
    12781 ggatttcata aagacgtgca atggcattat caatatcata ctctttgtca ctaacaaatt
    12841 ctcgagaaaa actgtttaat aaagaaatct tatttaagtt tatacctctt gattgaatcg
    12901 ttttcataaa cgaaaagcca tcaaatttct catctgaaat aattattact gtaggagttg
    12961 taaaaatatt tacatgataa aaccctcttg acctgcatat tctaaggaca ctgtcttcaa
    13021 ccctagatgt ttcagctcca ttttttatca tcagttcccc tacaaataga gctagtttta
    13081 gaatattttt cttatattca agttcctcat tttgattcat aaatacacct ccaaattaaa
    13141 aaacgtaata ttgaaattat atcaaatttt taagtaatat tccttaatat ctatacaaat
    13201 atattatctt ttttttgttt caaaatagtc acatttaaat tatatccaat taaaagaatt
    13261 agacaatatt atttattata ttttaactta aaaataaaga aagtaactat gttaaatatg
    13321 gaaaaatggc aaaacacttg actattaatt ggtaaaggac tataataata aattatcata
    13381 ttgataaaaa cgaaaattat gaaatgtctg taaaaataaa atttaaataa taactaggag
    13441 gagtaatatg tttgagaact tgaaaaaagc ggaccctgag atttatgaaa gtatgaaaag
    13501 agagttaaaa agacaacaaa gaaatattga attaatagca tcagaaaaca tagtatcagt
    13561 tccagtaatg gaaactatgg gaagtcatct tacaaataag tatgcagaag gttactcagg
    13621 caaaagatat tatggtggat gtgaatacat agatgaagtt gaaactcttg caatagatag
    13681 aatcaaaaaa atatttggag cagaacatgc taatgttcaa cctcactctg gagcaaatgc
    13741 aaatattggt gtatattttg ctatgttaaa accaggtgat gttgttatgg gaatgaattt
    13801 atctcaaggt ggacatttaa ctcatggtgc tccagttaat atttctggtc aatattataa
    13861 attttatgaa tatggtatag ataaagacag tggacttata gattttgatg aagttagaaa
    13921 gatagcacat gaagtaaaac ctaaaatgat agttgcagga gcaagtgcat atcctagaga
    13981 aatagatttt aaaaagttta gagagatagc tgatgaggtt ggagcattgc ttatggtaga
    14041 tatggctcat atagcaggtc ttgtggctgc tggtcttcac caaaatccat gtgaagtggc
    14101 tgattttgtt acaacaacaa ctcacaaaac tttaagagga cctcgagggg gtgttattct
    14161 ttgtaaagaa aaatatgcta aagacataga caaagcaata ttccctggta ttcaaggtgg
    14221 acctcttgaa catataattg cttctaaggc agtttgcttt aaagaagctt taagtgatga
    14281 gtttaaagaa tatcaggtac aagttgctaa aaatgcaaaa gccttagctg aagaacttat
    14341 taaaagagat tttaaattga tttctggagg tacagataat catttaatac tattagattt
    14401 gactaataaa aatataactg gtaaagcagc agaaaagaga ttggatgatg cttatataac
    14461 agctaataaa aatacaattc catttgaccc aaatggtgct cttattacaa gtggaataag
    14521 acttggtact ccagctgtaa ctactagagg tatgaaagaa gaagatatgg caataatcgc
    14581 tgaggctata gacctatgtt taacttatga tgaagaatct aaggctagaa cattggttgt
    14641 tggtttaact gaaaaatatc ctttatatga agaatataat attatggatt aattataaag
    14701 aacaactaaa taaaatacat ttaaataatt acaatgattg tgtatgtatt tgaatatagt
    14761 tagatataaa aaaggtgtct caaaatgaac tttttagttt atgaggcact tttttgtatg
    14821 gaatcgaaca tatttgtgtt atttaaataa taaaaaatat ggttatctct atttttagat
    14881 ggtctctttg tattttttaa ttaaaacttt aagatttctt aagaattata ctaggttttt
    14941 ctaaagattt aaagtctata cttataagta tagtaatcat atgaaaagaa aagctttaac
    15001 gaggagggaa tcttatgaag gtattaattc ttacaggaaa atttggtatg ggtcattact
    15061 cagcatcaaa ttctttaagt gaagatataa aagcaaaatt tgataattca gaaataataa
    15121 ttaaagatat atttgagtac attatgccta actattcaga taaaatgtac aagacttttt
    15181 ctattcttgt aaatcgggga agtagtcttt ataatttatt ttataaatgt gctgaaaatg
    15241 gaaagaaaga tataaaattt actttttcag attatttttt aaataaatta gatacattac
    15301 ttcatgaagt acaaccaaca gtagttattt caacttttcc attttgttct caattagtat
    15361 caagatataa ggaaaaatat aactcaaatc ttcctttaat aacatgtata actgatataa
    15421 gtagtcactc agaatggatt agtaaaaata cagactgtta tttagtagct agtaaatcta
    15481 ctaaggaaga acttgttttt aaaggaatag atgaatctaa gattaaagtt aatggtattc
    15541 cagtaaaaaa agaatttaaa agaatagaac atgtaaatca ttcaactaaa aaaaatattc
    15601 ttataatggg tggaggatta ggattactgc ctaaatcaga acaattttac aaagaattaa
    15661 atagtttaga aggagtaaaa actactgtaa taactggtaa taataaaaaa atgtactata
    15721 aactatatgg tagatatgaa aatatagagg tagtaggtta caccaatgaa gtatataaat
    15781 atatgaaaga ttctgacctt attatttcta aaccaggtgg aataacacta tttgaaacaa
    15841 tttattcaga acttccaata ttggcattta atccattttt acaacaagag atagataatg
    15901 ctagttttat attaaataat gaaattggga gaatacttgg taaaaataaa aaatactatg
    15961 tagatgagat aaaagattta atttatgatg atgctactct taaagaaatg agcagtaaca
    16021 tgaaagaatt aaaaaaacaa tttgataata ataccttaga gaatatttta ttttcacttg
    16081 atgaacaagg agcgtgtaga gaatgtatgt agtaggttta attatcgttg ttgcattgat
    16141 ttttttagtc cattcaatta tacctacata ctataataaa ctattaaata aagaggtatt
    16201 aaaaaatatg gctggagaaa atgaaattgc acttacattt gatgatggtc cagataagag
    16261 atatacagaa aaattactag atgtgttaaa agaaaatgat atacaagcta tgttttttgt
    16321 agtagcaaag aatgctgaaa aagagcctga aattataaaa agaatgttga gagaaaatca
    16381 tatagttggt ttacattcat tggaacacag aaatgcatgg ctttatagtt atagttatgt
    16441 taaaaaagat tttattgaaa gtacaaatat aatgaaaaat ttaggtgtag atgtaaacta
    16501 ctatcgtcca ccatggggtc atactaatat attttcaaat tcatttgtta aaaaatataa
    16561 tctaaaaatg actttgtggg atgtaatggc agaagattgg gagaaagata gtacagtaga
    16621 cattattata aacaagttaa tgagtagaac aaaagaaaat tctataattt gtttacatga
    16681 tgcaggtgaa aattcaggtg gtgcagtagg tgcacctgag cgtactattg aagctttaaa
    16741 gatagctatt ccaaaactta aagccagtgg tttaaagttc gtaacaccag aaaggatgta
    16801 gttatgataa gtaaaactga aaagaaaaat aaatgcctag gtagtgtagc ttttttagtt
    16861 ttactaatgg gcataacagg atattttgtt tttagagggc agtcagttga atcattgata
    16921 aaatcattaa aaggtgcaag ccctatgttt atactaattg gatttgcaat gatgtttata
    16981 tatgttgcct gtgaaggtat aaatatatac ttaggtatga aagctttaaa ccaaaagact
    17041 actcttttaa aatgtatggg atatgctttt attggatttt attttagttc tattacacct
    17101 tcagcatcag gtggtcaacc agcacaagtt tattatatga aaaaagacga tataaatata
    17161 tcatattctt cacttatact attagtcatt gttgttattc accaagtagt tatactggca
    17221 tatagtggaa taatgttcat tatggaacgt gagtttatat taaataatgt aagtggtatg
    17281 aacattttac taatatatgg tgtaataaca aatgttgctc ttgtcattgg agtaattgca
    17341 attatatttt caaaaaaact tgtaaacaat tttataatat caataacaaa tttattaggt
    17401 aagttaagaa taataaaaga tgttgaaagt tcaagaaaag ttataatatc tcaaattgaa
    17461 gagtatgtta aaggtgctca atatataaag cagaatccaa aacttgtagt ccaaatttta
    17521 gtcataacta ttgtacaaat aacagctatg tttttagttc ctttctttgt atacaaagct
    17581 tttcatctat caacatatac tgtatttgag atattagcga tacaatcctt gcttaatatt
    17641 gctgtttctt cattaccatt gccaggggct gttggagctt ctgaaaatag ttttatgaca
    17701 ttatttaaga tatttttccc tggtcactta ttagttccgg caatgttact tagtagggga
    17761 ataagttttt atgcatttgt agctataagt ggattgatat gtatagtagt acatgtaaaa
    17821 tcttctgaaa aagtaaaagc agtgaaaaaa gttgcttatg taagataata ttatatagat
    17881 tatttttatt aatatgtcaa tttgtgtgta tattttaatt aagctttaaa catatttaca
    17941 ggagtagaaa ctattaaaac gtgtgacatt gctaagcttt tgctaataaa gtataatatt
    18001 ttatatagtt tgaaatattt aagaaatata gcaaaaaaag gagcttggtg atgaataaag
    18061 agattccaaa aaaaatttta gtagctgatg ataatcctga gattagggaa atcgtagaaa
    18121 tacttttaac tggtgaaggt tatgaagtta ttatggcaac gaatggagaa gaagctgtaa
    18181 acttagtaga tagtacgatt gatttgatta ttttggatgt tgtaatgcct gtaaaaacag
    18241 gatttgttgc atgtaatgaa attagagaaa aaacgacagc accaatatta tttttaactg
    18301 caaaaactca agactctgat aaagttattg gattttcagc aggaggagat gactatttat
    18361 caaagccttt ttcatattct gaactaattt caagagttaa gtctctactt agacgttatt
    18421 atatatacca gggaaaggaa aaggaaagag agccaaatat attaaaaaca aaagagttat
    18481 gtgtcaattt agatactcaa gatgttacac taaatggaga acctgtgttt ttgacaacta
    18541 tagaatatag tatattaatt cttatgttga aaaatagaaa aaaagttttt tcgtctgaaa
    18601 atttatatga aactatttgg gatgagcagt atttttatac agcaaataat acaataatgg
    18661 ttcatattag aaatttaaga aaaaagttag aaaaagacca aaaaaatcca caatatataa
    18721 aaaccgcatg gggaaagggg tattatattg attaacaaaa ttaagccatc taagttggga
    18781 gttatactat tgatatatgt agtaatctca gctgcagtgg ctttcatttt aaatatacca
    18841 ttttactctg ctgttgaatc tatagtaaaa gatgtatcag aagggaaagt atattttaaa
    18901 agagaagtaa atcaacttat ggataatttt caaacttatg tgaataacaa tggtataaga
    18961 gcagatgatg gagatgatat agcaaaatgg aatagtgata attggtttgt ttctatgaag
    19021 atatataaag gaaataaggt tttttatgat acattatact atcctaatga agtacctgat
    19081 aagagtgagg aaaatgagtt ttatagcagt cccatggcta gaaactatga gatacaattt
    19141 tctgatatta aagctaatat agttgtagta gctttattta gatttagatt tcaaaatttt
    19201 atagaagcaa tttctattac atttatggca cttacattta ttataacatt tttactgttg
    19261 tttagtagaa agataaagta cctaaggaaa atagaaatgg gtataaagat agtagagagt
    19321 ggcagtttaa agtatagaat accagtaaaa ggtaatgatg aattatcatc acttgcatct
    19381 agtattaatg agatgagtca tacattggag aatcaactta taaaagaaag ggagatgaag
    19441 aataaaaata aagatatagt aacttcaata tcacatgatg ttagaactcc attaacgtca
    19501 gtaatttgtt atttagattt gattaaagac cataaataca atagtcctga gcaattggaa
    19561 caatacataa aaaatgtaag gtctaaagca tatcaaataa aagatttaac agatgacctt
    19621 tttacgcatt ttgttaattc tggagaggaa gtttcaaaag ggtatgaaaa aataaatgct
    19681 aatgaattta tttttcaatt acttttagat agttcatata cattggagga gaaaggattt
    19741 catataacaa tagaaaatga actttataac caatatcata tttatgtaga tgttacacaa
    19801 tttagaagga tatttgataa tttatgttct aatataataa aatatgctag gaaagataag
    19861 cctatagtat ttttaattaa agtagaggat tctaaattac taattataca aaaaaataga
    19921 gtaagtaatt tttctaagaa tgtagaaagt tttggaattg gtataaagaa ttgtgagaaa
    19981 atagttgcat atcataatgg aagcataaat gtaaattcag atgataaaga atttgctatt
    20041 gaaatatcta ttccagcaaa agaatcataa aaatttaaat tatatattgt gtaattgcta
    20101 ttatatattg tgtaattgct tatgtaaaat tctatgaagc aggaagcatg tgactttagt
    20161 tatgggatgt tcaatattgt gtacctctag gcaagtaaca agtttactta caagaccata
    20221 taatattatt atcttaaaaa aaggggacat aataatatgg ttttgacagg agcacattat
    20281 atttacattc tatttatggt aattatatta attactatga ttatgaaaaa ggatacaata
    20341 gtaccatgta tattaggtgt tttttttatg gggttgttct ttgaaaaaaa catttttggt
    20401 gctataacag cagtgtttaa ctcttttatt atatctttaa atgagttggg tccaattatc
    20461 ttgattatag ctattatggt tgcattatct aaagccctag aagccaataa tgctatacag
    20521 tatatggtga gaccattttc gagagttata aaaaattcta atacagcatt ttttgtaact
    20581 ggatttgtta tgttagttct ttcttggttt ttctggccaa ctccagctgt tgccttagtt
    20641 ggagctgtat ttttacctgt tgctatgaga gctgggctac cagcaattgg tgttgcagta
    20701 gcattaaatt tatttggtca tggtcttgcc ttgtctacag actttgttat tcaaggagct
    20761 ccaagtataa cagctggtgc agcaggtgtt gcagtatcat atgttataaa tgatggtatg
    20821 attttgtttt gggtgatggg aatcgtaacc atatctatgg cattttatac attaaaaaga
    20881 gatattaaca aaggaatgtt tagagaagaa ctaaaaggtt ttgaaagtga agaagtagaa
    20941 gagttcaatg gaaaatctaa aatagcaact attttagtag ctctgggatt tcttgctgat
    21001 ataattgcta tgtatatatt tgacctaaaa ggaggagatg catcagctct tttgggtgga
    21061 acagcagtat ttttaataat aattataaat gttataaatt ttggcaaaga tagtcttgaa
    21121 aatgtatgtg agaatataat tgatggtttt gtgtttggga taaaaatatt tggagcaatt
    21181 ataccaatag cagcattttt ctatatgggt gaagttgcac cattgacagg tgtatttgga
    21241 aaagtattag caccaggttc acaaggttta ttatcagata taggcatagc tctttctcaa
    21301 acaataccac ttaataaatt tgccgtatca ggtatagaaa ctgtagtggg agcaataact
    21361 gggcttgatg gttctggatt ttctgggatt tcattagttg gttcacttgc ctctgtattt
    21421 ggaactgcta taaatgcaag tgttggtgca ctagctgcat taggacaaat cagtggtata
    21481 tgggtaggtg gaggctgtct agttccttgg ggtcttattt cagcagcggc aatttgtgga
    21541 gttagtccaa tagaattggc aaagcgaaac tttgtcccag ttataaccgg attggttgta
    21601 acaaccatag tagcaatgtt tattatttaa taaatcgaat aaatctttaa ttgtataaat
    21661 taaaaataga tgtgtagcta taatttatta taattgtttt actaattcta agccatacat
    21721 ctatttttat tgatattttt attagtattc tttaataaaa taatattttt cattatacta
    21781 tacataacat ctaaatataa catctaaaat actgttatct atatttaata tcttgccaaa
    21841 ttttttgatg ttttattgcc ctgtcccacc aacatatata agctatcaca agtttttacc
    21901 tatactatca ccaaacaaaa attctaattt taatttatta ttttataacc tatattatat
    21961 taactatatt agtttgtaga ctttagatgt aattataaat attaatactc caagagttca
    22021 aattcatatc ctagttttct tgcatttgat ataaaatcat ttaaaatttc tgtactagtt
    22081 ttggaaactg catgtagtaa caagatagaa ccatcatgta gattatccat tattttttgc
    22141 gttgcttctt catgtgatgg ttgtttatca gtatcccagt ctctgtatgc aaaactccaa
    22201 aatacagtct tgtatcctaa atttttagtc attgccaagc ttttttctga gtattttccc
    22261 ataggaggtc taaaaaattt aaccatatct ttcccagtta catctttata taacttttca
    22321 acatcatata attcgtcatt aaagtttttt agattgcttg tttttgttgg catagatgga
    22381 tgggttttac tatgatttcc aactatatgt ccctcagaaa ccattctttt tactaaatct
    22441 ttattttctt ttatatatgg agctgtaaca aaaaaaactg ctttaacctg attttgtttt
    22501 agagtgtcga gtatttttgt agtgtatcca ttttcataac cttcatcaaa tgtaagatat
    22561 agtgtttttt gaccatcttt tgtaggtcca ttgtataggg catcatagtc agaaaattta
    22621 aattcaatgt ccgtatttac ttctggtgtt ttatgcttat tgtttggtat ataaaaccaa
    22681 tcaagagtag tgttatctaa agaagataca tctaatttat tattatcatt attagtagtg
    22741 gcttttgatg gtttatctgt tccgtctgaa actattacat tggaagtatc tttaccacta
    22801 tctatttttt ccttatcttc ttgtaactgt gtttccttat tttgattttc gttttgattg
    22861 ttttgtgaat ttgaacatcc aacaataaaa aaaatagaaa tagaaagtag tgttaaataa
    22921 tgcaatactt ttctaaacat tttgaagctc ctttctaaat taataataat tgcatataat
    22981 aataattata tgattaatta caattgcata tagtattatt ataattggaa attactatta
    23041 ataatagtta tatctttaaa aataaggtca caatagtatg acaaagggtg aataatcaat
    23101 aaatttaaaa aaaaatgaaa aaaatagttg accatattat gagaagatgt tattataata
    23161 aatgtgctca acaagaagta acaaaataac aagcacaaag aagttaattg aatgaaatga
    23221 actttgaaaa ttaaacagta ggttaattta taaaacaaga aacaaaccat aaagccagat
    23281 attttgataa caatagtatc tgagcctgat aaacttttat ttgagagttt gatcctggct
    23341 caggatgaac gctggcg
//
LOCUS       DAELTA010000047        22908 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.047, whole genome shotgun sequence.
ACCESSION   DAELTA010000047 DAELTA010000000
VERSION     DAELTA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.047"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(215..1264)
                     /locus_tag="KPG13_001604"
     CDS             complement(215..1264)
                     /locus_tag="KPG13_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985337.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEI
                     NVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITV
                     MKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMK
                     EIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            complement(1494..2579)
                     /locus_tag="KPG13_001605"
     CDS             complement(1494..2579)
                     /locus_tag="KPG13_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG0985338.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2615..3928)
                     /locus_tag="KPG13_001606"
     CDS             complement(2615..3928)
                     /locus_tag="KPG13_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0985339.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3929..6220)
                     /locus_tag="KPG13_001607"
     CDS             complement(3929..6220)
                     /locus_tag="KPG13_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0985340.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6210..7004)
                     /locus_tag="KPG13_001608"
     CDS             complement(6210..7004)
                     /locus_tag="KPG13_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0985341.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            complement(7043..7489)
                     /locus_tag="KPG13_001609"
     CDS             complement(7043..7489)
                     /locus_tag="KPG13_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0985342.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7561..8967)
                     /locus_tag="KPG13_001610"
     CDS             complement(7561..8967)
                     /locus_tag="KPG13_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0985343.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            complement(8985..10355)
                     /gene="hydA"
                     /locus_tag="KPG13_001611"
     CDS             complement(8985..10355)
                     /gene="hydA"
                     /locus_tag="KPG13_001611"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0985344.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10488..11675)
                     /locus_tag="KPG13_001612"
     CDS             complement(10488..11675)
                     /locus_tag="KPG13_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBG0985345.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11802..13016)
                     /gene="dpaL"
                     /locus_tag="KPG13_001613"
     CDS             complement(11802..13016)
                     /gene="dpaL"
                     /locus_tag="KPG13_001613"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG0985346.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13379..14050)
                     /locus_tag="KPG13_001614"
     CDS             complement(13379..14050)
                     /locus_tag="KPG13_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0985347.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14133..16349)
                     /locus_tag="KPG13_001615"
     CDS             complement(14133..16349)
                     /locus_tag="KPG13_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0985348.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16352..16822)
                     /locus_tag="KPG13_001616"
     CDS             complement(16352..16822)
                     /locus_tag="KPG13_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG0985349.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(16884..18278)
                     /locus_tag="KPG13_001617"
     CDS             complement(16884..18278)
                     /locus_tag="KPG13_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0985350.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18351..19712)
                     /locus_tag="KPG13_001618"
     CDS             complement(18351..19712)
                     /locus_tag="KPG13_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0985351.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20009..21331)
                     /locus_tag="KPG13_001619"
     CDS             complement(20009..21331)
                     /locus_tag="KPG13_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG0985352.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21687..22727)
                     /locus_tag="KPG13_001620"
     CDS             complement(21687..22727)
                     /locus_tag="KPG13_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985353.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6812 a         4636 c         2525 g         8935 t
ORIGIN      
        1 agcgttctat tagattttat catctaaatt ttaggtttta tttttataaa ttaattgtat
       61 ttacataaaa aaaaagacct aaaaacaaat ttttaacggt cttttctcaa ttttttattt
      121 tcatttctaa aatttataat aaatagtaag aacactataa attcttttat ataaaattct
      181 tagtttgtgg atgataaatt agtgaattaa aatactactt tactgatttt agtttgacaa
      241 tatcactcca atttttacta gtggctaact ctactaaatt taagtctctt cttatttcag
      301 ctatatcctc ttttatagaa ttagtgtcac cttttatata tgctatgtta tgttctatat
      361 tatcatgagt agctttattt atttcagaat tatgtaataa acttcctaaa atattattct
      421 gttctatctg tatctctttc attgatttat cttcacattc tagactatca atcttaattt
      481 caattccata taatttttca tttgctatgg atacctcgtc cttaattcca ttcatatctt
      541 cattaatgtt atttatttta ttttgcataa agtttacttc tgatttaact tcggaaacat
      601 cttctttcat tacagttatt tcttgtctca cttctgacat gtcttttttc attacattta
      661 tctcttgttt tacctctgat gtatcttctt tcattacggt catttcttgt cttacttcgg
      721 acatatcttc tttcattata tttatctctt gtcttacttc ggacatatct tcttttaata
      781 catttatctc ctgttttact tccgacatat cttctttcat tacagtcatt tcttgtctta
      841 cttccgacat atcctctttc attgtattta tctcttgtct tacctcggac atatcctctt
      901 tcattatatt tatctcttgt cttacctcgg acatatcttc tttcattaca atcatttcct
      961 gttttacttc tgacatatct ccttttaata catttatttc ctgttttact tccgacatat
     1021 cttctttcat tacagtcatt tcttgtttca tttccgacat atcttctttt aatacattta
     1081 tttcctgttt tacttccgac atgtcttctt tcattacagt catttcttgt tttacttccg
     1141 acatatcttc ttttaacaca tttatttctt gttttacttc cgacatatca ttttttatgc
     1201 taactacatc ttcatttata gtatataata actctaaaat cttctcttgc acttgcatca
     1261 ccatccctat taattattat aacattttac acaaatgcta tacaatgtta acatatatat
     1321 cagaattatt aaaattttaa ttaaacaaaa ttgattcact ttattacttc aaaactaatt
     1381 aatatctaaa ataactacaa ataaacaaaa ataaggtcaa caacaaaaaa ggcttatctc
     1441 tttgagatag cctttcttat ttttatttaa aactttgttt tttataaaat aatctataca
     1501 ttcagctctg aacatgaaat ttttcttcca agtgcttttg gtttacaaac tgatatacaa
     1561 agtccacaat atctacactc ttcttcattc aattccatct tatcattcaa ttttctcgct
     1621 tgatatgcac aaactctctc gcacatctta catttagtac atttttcttc atcataagta
     1681 aactcaaagc taagtctttc attaacatct cccatatgat tatgtactat accacaagca
     1741 ttttctatag tattatatcc taatctatta agattagatt ttaaatcatc atgtatttta
     1801 gatattactt gtgcaccttt taaaattact actgaacata taccaacaaa gtcagcacct
     1861 gccataatca tttctaaggc atcatctcca cttacacatc cacctaagcc aattattggt
     1921 atattaactt gtttttttat ttcatatatt ctctgtaaag ttattggctt gatagtttct
     1981 ccacttaaat atcctattcc atttccacct aataaaggtt gccctgtttc tatgtctatt
     2041 ctaaatgctg gtcctacaga gtcacaagca gttattgcat ctgctcctgc ttcttctaat
     2101 tttttagcaa aatcgcctat ctcatctatc ataggaggaa gtttaactat tacaggtata
     2161 tttactcttt cctttgcatc tttaagcatt ggtatcaaat ctctatagtc ataagatact
     2221 aattcaataa agtcagctcc tgcatttgca actttctcta ctaattcgct actttcttca
     2281 attgtatgac ctattgaagc tatacagaca gtcccatctc ttttcatttg tggtatttca
     2341 aaatctatcc attgctctgg ctcaaaatct gtccattttt cattatttaa aagagcatta
     2401 gctgtatttc taaccatatg tggtgctgga tttatagctc tttcttttct tatagtctta
     2461 gtcacaactg cccctgctcc tgcatcactt aatatcttgc atccagctgc actatatgtt
     2521 aatggtcctg aacctattat aagtggtgat tttaattctt tacctaaaaa attatacatt
     2581 atctgcttcc ccttttcata tatatttaca ttcgttaaaa tttccaaaat tcacaagcaa
     2641 cttctttcat ttctttaaca atagcttttt tatctaaatc aactaactgt ttttctttta
     2701 aaatatgtct accatctatg tatacatttg atataaattc ttttttaccc tgaactacta
     2761 tctggtcaaa tatattatca agagtcacag gagttttaaa ttcatcttcc ataactatta
     2821 tatcagcctt attaccaact gctattttac cagaattttg aagtcctaat acataagcac
     2881 catgctctgt tgccattcta aatactaaat ttgctggcat tactgatgca tcttcttcaa
     2941 ctgatttatg cattaaaaat gcacctctca taacttcaaa aaaatcattt atatatccat
     3001 cagtacccag tgccactttt atgccttttt taatcatttt tggtactgga gaaaaacctc
     3061 cacctacttc acagttacta agaggcatat gaaccacttt tacgcctttt tcttttaata
     3121 tatctatttc ttcatcattt acttttacac actgagatgc taatacagtt tcatccaata
     3181 tatctaaatc attatagtag tcaactgctt ttttgccaaa gtgtttttca gcatagtttg
     3241 gctcatatat actctcacat aaatgaaatt gcattggtgc atttaatttt tttgcatctt
     3301 ctttagcttt cttaataaat ctatcagaac atgtgaagct tgtatgtgta cacattattc
     3361 catttattaa tttactattt tctctactcc actttattaa attagaattt tcatctaaac
     3421 atctaagtcc attttcaaag cttattcttt cacaactttc taaggataaa atcgctttca
     3481 tgcctatttc ttctagtatt tttccttgct caattaaagt tccttcttct gtatttggag
     3541 cttctagggt atcacaaaaa gctaccacac cagattctat aagttctatt gctgatgctt
     3601 tagttgttgc cttcacttct tttaatccaa ttctattttc tataaatggc caccaatagt
     3661 catttaaaaa accttcaaag tcagtaaaat gtacatttgc cggtattccc ctagaaagaa
     3721 ctccatattg atgcatatga ccattgacga atcctggcat tattatagca tctgatttat
     3781 ctattatttc ttcaatattt ttatatttta tttcaacttc ttcatttggc aatatatctt
     3841 ttataatgtc attttctact acaacagcag aatttgtata tatttcatca gcagaagata
     3901 ttaagtattt acttttaata gcaatcattt atttacctcc ctgatttaat tttttcatgg
     3961 ccatataaac tttctcagaa gttataggaa gttctcttat acgaactcct gtggcattgt
     4021 atattgcatt tgctattgct ggtgcaggtg tatgaataac tatttctcct aaagatttag
     4081 ctccatatgg acctgttggc tcataattat ctacaaaatc aacaattatg tttcctatat
     4141 ctttttttgt tggaactttg tattgtaata gattagaagt ttgaagcttt ccatcagaac
     4201 catgtctagg gtcttcatac attgcaagcc ctataccttg agttacacca ccttcaactt
     4261 gtattctagc aagtgcagga ttcataactg taccacaatc tgtagaacaa gcataattta
     4321 ttactttaaa ctcccctgtt gttttatcta actctatttc agcaaatcct gctaagaatg
     4381 gtttagcact ctcttgtata ccataagatt cagatgaata taaaacttct tgatttccac
     4441 caccaacaga ttcttttcct aattcactta acaaaacaaa cttctctaca tcttcactat
     4501 aacaaactct gtcttcatat agtattaaat catctattgg taaatcgagt ttcttctgtg
     4561 ctacttttaa tatcttttgt tttaaacttt cagcagattt tattgtagca tttccagtta
     4621 cataagtagt acaagaagcg tatgcaccag tatcatatgg acacatatct gtatctcctg
     4681 tataaacgga aattctatca atagatgtat tcaatgcttt tgcagctatt tgtgcaagta
     4741 ttgtgtcaga accagttcct aagtctgaag acccagaaaa caacttataa gttccatctt
     4801 catccatcct catatttact attgccatgt ctatgttggc tataccagaa ccatgagttc
     4861 ccacagacat tccaacagcc ctcaccttat tgtttccaat atccctaaca gggtactttt
     4921 catcccatcc aataagtttt ttacctctaa ttatacattc atcaagtgca caacttctta
     4981 ttggaaaatt cattattccg ccttcagttt gtttttttat aatattcttt aactttattt
     5041 ctgttgggtc catattaagt ttatgcgcta attcatcaat tgcacaatca agtgcaaatg
     5101 taccttgtgt tgctccatat cctcttaatg ctcctcctgg aactaaatta gtatatacag
     5161 ttttcccatc aaattttatt gcaggtacat tattatatgt tggaagtaca ttatgacctg
     5221 attcagagca aactgaaggt ccattatcgc cataagcgcc tgtattgttc aatgctttca
     5281 tatctatagc ttttatgttt ccttctttat ctgccccaat ttttatatca aaaaacatct
     5341 gatgtctagt attagtcatt gcaaaagttt cttctctagt ataaattagt tttgctggtc
     5401 tttttgtcat ccaagttaca aatgatgcat atatctctgt tacagctatg ttttttccac
     5461 caaaacctcc acctattctt ggttttatta ctctcacttt tgaaagaggt attcccagtg
     5521 ctttagaaac ctgtcttctc atatgataag ctgattggtt tgcagaagtt actactaatc
     5581 tgccattcgt atctatatac gtataagctc tatgagtttc catcatacaa tgggcttgtg
     5641 attgagtcat ataagaatgt tctactatta catcactttc tttaaactct ttttctacat
     5701 cccctttacc cattttttct ctcgatacaa tattttttgt agcatccaat ccaaaatcaa
     5761 atggacaaaa caaatcatct tcttcatgga ttaaaatttt atttccttcc gattctcttg
     5821 catccaatat aggagtcatt acttcatatt caacttttat aagtttcata gctttaatag
     5881 cagccctttc atcttcagcg gctattatag caacttcatc tcctacatat ctaactacgt
     5941 cttcaagtat taattggtca tgaggtgatg cttctggata tgactctcca accattgaat
     6001 atctataatt tggaacatct ttgtaagtga atatgtttac tacacctttt acctttaatg
     6061 cattttttat atctatgttt tttatcttcg caaaagcatg tggacttcta agaagcttaa
     6121 ttgttaaaac atctttatga aaaattaaat cttctgtata tattggtttt ccagtaacaa
     6181 tggcttcact atctattttt cttacctctt tatttaccat cacactcacc tccaatacta
     6241 ttatacattc tcactactaa ggcttttgtc atatcttttc tgtattcttt acttcctctt
     6301 atattacttc caaatgaaag ttcttcactt gatacttcac cagctttttc tatatcattt
     6361 tctaaactta atatttcact tgctttatag gcaatttttg ctcttcttgg tctagcgcct
     6421 atcgcaattt tataaatact tccttctttt aacattgctc catttaatac tgcaaaatca
     6481 cctgtacatt ttcttataga atcaaataca cctatagcat cttttttagg taatataact
     6541 tctattaata tatctttttc aagctcattt tctaaaaaat cagctaagtt cataacccct
     6601 tttttatata acttaacctt tgcatctacc actagtaatg aaggtattaa atctgaaaat
     6661 ccatacttag caaatacgct agctcctact ctagctccac ttctaaattg aactcctact
     6721 atattagaaa cagcgtttga aattacccca ttacagtaat ttttaataac cttatttaat
     6781 tctaaagttc ttaaacttgt atctgctcca attaatatgc tatcatcagt ttcatttata
     6841 taatctaaag ctaagtcttt taaatctatt gctgaaccaa tatttttact acctagtttt
     6901 aaaaatccac atcctccaag tattatgttg ttttttcttg atattagcag ttcataagct
     6961 tcatctaaac tttttggaac tacatattcc tttatagtta tcatactctc tccccatatc
     7021 aacttaattt tcacttaaaa gtctaagtgt ttaaatattt atgtattgct cttaattgac
     7081 ctttatatcc tgtacatcta caaaggttac catttacata atctataact tcttcttctg
     7141 ttggattttg aagctctctt ttcattgcca agaccatcat tataaatcca ggagcacaaa
     7201 atccacactg gtcagcccct tcgtctgcaa gaaaatctcc aaacttcttt gcttcatcca
     7261 agacaccctc aagtgttgta atatgctgac cttctactct agcagtaaga taattacatg
     7321 aaagcacggc cttgtcattt accatcactg tacataaccc acatgagcct gtatcacacc
     7381 cttttttaac acttacatat ccattttctc ttaatgtatc tgctaaaaaa tcatctggtc
     7441 ttatacttaa ttctttttct ttattattta tggtacattt gataatcaaa taaatcacct
     7501 caaaactcat atttttaagc taaagtttta ctatactttt ttatgaataa attacattta
     7561 ttaataaagg ctattccatt tatgaccttc cattctattt tttatagttc ctcttataca
     7621 aagtctctca gctgtatctt gagcatcttt taatgctttc tcttcattag ctgtcaatat
     7681 ttttgaatct tccattatta tatttccatc tactataaca ctctctatat ttttcatact
     7741 agaagaatat actaatgtag ataccggatt atgcataggt atcgcttttg gtgaaagcat
     7801 tggattaaat attagtaagt ctgctttttt acctatctcc aatgaaccta tctcatcttc
     7861 cattccaata gctcttgctc catctatagt tgccaactca agaacttttt ctgccgacat
     7921 tgctaatggg tcacacttat taactttatg ttgaagtgct gttaatttca taagttcaag
     7981 catatcttga gagttattac tagcggctcc atctactcct aaactaactg ttattccttt
     8041 tttaagcatt tctggcactg gtgcaacacc tgaagataag tacatattac ttgctgtatt
     8101 atgagacact ttcatatcgt attttttagt aagttccata tctttttcag ttagatacac
     8161 acaatgtacc ataagcacat ttggtccaag tattcctaat ttttctaaaa catctatgtc
     8221 atattgccca tgcaattctt ttgctgcttc tctatcaaaa ggagtctctg atatatgtac
     8281 tgtaaataaa gcatcatcat attcttttac tactctccat agcatttcaa gcatttcttg
     8341 agaatttgac catattgctg caggagctac tcctattttg attcttccat tttctgtatt
     8401 atggtgtttt tcaaacaatc ttcttacatc tttttcaacc gtttctacat cttgcattat
     8461 tccaggatga actccaaatt gagctcctgt attcatacat cctcttccaa gtattcctct
     8521 tattcctaat tctttataag catctattat tccatcacaa agacctggct tactatgagg
     8581 atacatgtag tcaaccattg ttgttatacc acttctaagt ccttcaatac atccaagcat
     8641 tgcagcatca taagtatctt tgggctctaa atagttagct gctggaaatg tcatagtttc
     8701 taaccagtct ttaagtacca tatcatctcc caatcctttt aataaagttt ggaataaatg
     8761 attatgtgta tttataaaac ctgggaataa aactttacct ttagcatcta taattttttt
     8821 aacatcagta tatttacttt ctatctcttt agaatttcca atatcagcaa tcttgttatc
     8881 ttttactact aatgccccat caaaaataac ttctctctct ttattcacag ttactattat
     8941 ggcattctta ataagtatat ccatcattct tatttccccc cgcattattt agtaattttt
     9001 cttttaatat attgacctgt tggatgttct ttaacattac aactttgtac tacatgattt
     9061 cctctcaaaa atacatcttc aactttacat ttaaccttaa atccttcata tggagtataa
     9121 tctacatttt gtttttgagt tttataacta attgtttctt ctttattgat atttaaaatg
     9181 actatatctg catcacttcc tacagctatc tcaccttttt taggatacat tccaaatatt
     9241 tttgcagcat tcgtagaagt aacttccaca aacttattta cacttattct attttctaag
     9301 accccatatg tatataaaag tgctaaccta tgttcaacac caggtgctcc atttggtatc
     9361 ttactgaaat catcaattcc caagtctttt tgccccttaa aattaaatga acagtggtct
     9421 gtgccaactg tttgaatatc tccactagac aatccttccc aaaggtaatt tatgtcttct
     9481 ttttttctta aaggaggaga cattacatac ttagcaccct caaatttatc cttattatat
     9541 aaattgtctt ctaataatag atactgtggg catgtttcta ctatcatatc tatatttttt
     9601 tctctagcta tttttacagt ttcaagtgac tccctacaac ttaagtggac tacataggat
     9661 tttgaattag ataatttagt tatatcagca agtcttgaaa cagactcttt ctcaactatg
     9721 ttaggtcttg taagtggatg atattttggc tccaaattac cattttgtat attttcttct
     9781 atcaatacat ctagtaaatc cccattttca caatgaaatc caactataca acctaaatcc
     9841 cttgctttct ttaaaaccct gtatattgtt ccatcatcta ctttcatgcc atcatatgcc
     9901 atatacatct taaaagatac tatcccatct gaaataagtt tttccatatg attaaatgta
     9961 tctttattta aacatgtaat tgtcatatga aaaccataat cacaataaca atttccactt
    10021 gcttttttat gatatgcttc tacaccttgc agtagatttt ccccctcatt ggcttctgca
    10081 aaatctagta ttgtagtagt tcctccagct atagctgctc ttgttcctgt ttcaaaatta
    10141 tctgcagttg taatagaacc tgcattcata tcaaaatgag tatgtgtgtc tattccacca
    10201 ggaattacaa ttttacctgt agcatcaata accttatcac catttatttc taagttattt
    10261 cctatttcaa ctatcttttc atcttctatc cttaaatcac ttatataagt cttatcagag
    10321 gtaattatag ttccacctct tagtattgtt cccatatata cctcctaact tttaagcttt
    10381 aaagtatttt ctaagtattg taaaacttta tttaaaattg tattttctaa aggtgctata
    10441 ttcagcacct ctagactata tttaaataat ttattgtgaa ctaaatatta ttttccgtat
    10501 acagatttta atattccata gtaaccttct gttcctataa ataactgttc ttgctctata
    10561 tactcatcta ttgtgtgagc aagattttcc ttagaaggac caaatcctat agtttttatt
    10621 ccagcttcac ctgcatagtg acttccattc gtacagaatg agtattgagt tatttctgaa
    10681 tctattccag cttcttttaa tcccttatac gctgcttgaa caaattcatc ttcttcgtca
    10741 tataaccaac ctgggaagaa tctttcactt tctatagtat ttcctgtata gcaatcagct
    10801 ttttcaaccg cataagaaac ttttgcattt aattctgggt cttctttcat catttcatct
    10861 aataaagctt gtattggagc taatacgcct tctcttgttt ctccaactaa taatcttcta
    10921 tcaaaagtag ctttgcaata atctggcact actgatgctc ctggatatgg agaagatttt
    10981 atatctgtta aaactaatat tccatcacct aaaactggat gagttggagg tactaattgt
    11041 tgtatctttt ggattacatt agccatttta taaactgcat ttacaccttt ttctggatta
    11101 gcagagtgag ctggtttacc aaaagtttct actactattt ctcctctacc tctctgacca
    11161 atctttaagt ttaactctga agactcacct attacaacat agtcaggttg aacagcctta
    11221 cttatctctc tagccgaaac accttcaaat atttcttcat gaacaacacc tgctacatat
    11281 agttctccag caaaatctct attagtgtct ttagcaaaat acgctacagc agacatcatt
    11341 gcagtatatt gacctttcat gtcagaagtt cctctaccat atatctttcc atcaactatt
    11401 tttccttcaa aaggaggaac actccattta cttgcatctg gaactggaac tgtatctata
    11461 tgtccatcaa ataatatttt cttacctggc ttgttacctt ttatacatcc tactatattt
    11521 ccatatctat ctcttgacca actatcaaaa cctaattttt caaaagactc ttctattgct
    11581 tttacaacac cttcttcatt tccagaagaa ctaggatttt gaattaattt ttggcatatt
    11641 tcagttactt cttgttttct ttgttcgttt aacatttcat tatcccccta gattttttat
    11701 aatgtatgta ttttatatac ttatatattt tctaaatttt agaacttata tatcttctaa
    11761 atatgtaaac caatatctaa taattgcatt ttagtaattc cttatatact tggatattct
    11821 ccatcccaaa ctatacttct atatttgtca gggtcagtat ctccttctgt gcttattaaa
    11881 agtattcttg aattttcatc taattttaaa tcttttctta aatctgctaa atcttctctt
    11941 tccatagctg ctactaaaag accagttgta actgctccag attctccaga aattaccttt
    12001 tcatcaccat ttaaaggatt acctaatgtt ctcatacctt tagcagaaac ccagtctggt
    12061 gctgaaacaa atgctgctgc atgatttttt aatacttcaa atcctatagt atttggctct
    12121 ccacaagcaa gccctgccat tatagttggc atatctcctc caacaaatct tggttttcca
    12181 tctccagctt ctgctgattt atagtaacaa tcagcttcat cagcctctac tactactgtt
    12241 attgggcatt catcatatat tgaagcaaca tacccttgaa ctgcaccagc aagtgaacca
    12301 actcctgctt gaacaaatac gtgtgttggt ctatctactc catattcttt taattgctct
    12361 atagcttctt gagccatagt tccatatcct tgcattatcc atgctggtat ttcttcatat
    12421 ccgtcccatg ctgtatcttg aaccattact ccatggtcat cagcttctgc ataatcatta
    12481 gctaatctta ctgcatcatc ataatttaag tctgttatac tagcatctgc accttctcct
    12541 ttaattttat ttaatctaaa ttcagaagaa ccttttggca ttaatacaac agatttttga
    12601 cctaatttat tagctgtcca agcaactcct ctaccgtgat ttccatctgt agctgtaaag
    12661 aaagttattt ctcctaattt ttcttttatt tctttcgaag tcaatttgtc ataccctaac
    12721 tcagatatat ctgtatctaa tctttgagct aagtatctac ccatagaata agaaccacct
    12781 aatactttaa atgcatttaa tccaaatcta taagattcat cttttacata tactcctgca
    12841 acacctaatt tttttgctaa accttctagg tttgctaatg gagtttcttt atattgagga
    12901 aagcttttgt gaaaatttct aaccttagtt atttcttctt catttaaaaa tttgatacaa
    12961 ttttccttat cgccctttgg caaattattt actttccatt taatttcttt taacatctaa
    13021 aatacctcca aattattgtt aaatttttat actttaatat ataagcaatt aacatgccaa
    13081 tactaaatat atactagata tttttccatg actctttagt atagaaattg caatgctttt
    13141 attatttttt cttttctatc tttagttcat acaatgtatg tagaatttaa taactaaaaa
    13201 accttttcta tcttctcact ttttaattct catttttaaa atttaaaaat aaatctcaaa
    13261 acactttgtt tctatatttt ttaatataaa atttataaaa tctaaattaa aacataaaat
    13321 agtgatgcaa taatctatta catcactatt tgtatatatt caataatata ataaatcttt
    13381 agtggtctct aagaggtgat ttttcatctt ttttaacatc taattcttct tttacgacat
    13441 tccatttttc aagaccaaac ttagaaacca taactgcctc tgctgcacct tttccaagtc
    13501 tttttactgt gtccatacct gtctcatcct cttgggcacc ttttgctttt ccatctttac
    13561 tccatattgt accacctata tagcatccac ctaaacctcc agacactaac atttcatgca
    13621 ttaggaagaa atttattata ggaagttttg tcaattcttg gccaccatat ctagttccac
    13681 cagtagttat agctgcacca accttatttt gaagttttcc tggatgaact aaatacattg
    13741 gacgaagtct attaaatact gctgtaagtt gagcagttat attcatatca taaactggag
    13801 aacctactat aattccatct gcctccaata ttttttgctc taattcttgg atatcatctt
    13861 ttataataca catagtttct tttcttatac atgcatcaca atgtacacaa ggacttattt
    13921 ttttccctct aactgaccaa tactcagttt ctataccagg tatagtttct gcttctttca
    13981 aagcttctaa cactgcatat tctgttgctc ctttacgtgg acttccacaa atacctaata
    14041 tctttgccat aataatatct cctcaccaaa taaatctatt tttattatct atttacaaat
    14101 acaataaatt gttatttctg ctttaatttc tactatttta attttattgg taatgaatgt
    14161 atctctacat cagttgcatt atatactgca tttgcaacag ctggcgcaac tggtacaacg
    14221 gcacattctc caatactctt accaccaaaa ggcccagctt tttcataacc atctacaaat
    14281 gcaacttgac attttggcat atctacagtt ctaaataatt tatatgtttt aaaatcagta
    14341 gctttaggtc taccttgttc atcaaatgcc aattcttcac tcaatgcgta tcctagaccc
    14401 atatgaatcc caccttctaa ttgtccttct aaatttagag gatttataac tctaccaaca
    14461 tcatgtacag ctacaaaatc aactactttt atatccttag tttctttatc tactaaaact
    14521 tcagcaaaat gtacaccata agaagtacgt ccagctggtg aagaataatc ttcaacaaca
    14581 gttaactctc ttaatgattt actttgacta tatactgcga catcagaaag tgatgctttt
    14641 acattttcat caagtttact aattacacat ccattgtcta gatataaatc ttcttttgat
    14701 gtttcaagta atttttctgc ttcttctaat ataagatttt tcaatttctc tgctgtcttt
    14761 ttagctcctc caccttctac aaatacccct ctacttgcat agtcaccaag gttgaatgta
    14821 catgcatccg tatcagtttc aaatgtatca actttatcag gtgttattcc taaaacttca
    14881 gctaccatca ttgtttgcat agttacaaca ccattaccca tatcatgagc tcctgttagt
    14941 agtgtcaacg taccatcttc atttaattta agagtcaatg taacaacatc tctatgtgca
    15001 ccaaaaactc cattaccatg tgcacctatt gccataccta tacctcttaa atattttcct
    15061 tcttcttttg gaagtttttt cttttcatcc cacttaaaca tcttcttacc ttgctctata
    15121 caatccaata ctctaggatt tccaagagaa ccgtggaaga ttatatcgac atcatctggc
    15181 tctactgcat ttttattttg aaaatcgact aaatccatac ctatttcctt tgctattttc
    15241 gcaaattgag cttgttgagc cataaatatt tgaggtgacc catagcctct catagctcct
    15301 gctattggtg tatttgtata tacaggcatc ccagtaaact ttatatttgg tattttataa
    15361 actttaaata ctttatgact aagtgctcct ataacattta aagcacttga tgcatatgct
    15421 cctgtatttg tatatatttt tatgtcttgt gctattactt caccatcttt atttacacca
    15481 gtttttaatt ttacaaaaga cccatgacga gttcttgttg atatcataga ttcctttctt
    15541 gtaagaacca ttttaactgg tcttcttgtc atttttgata atgcggctgc tactggttca
    15601 attgtaagtt ctaattttcc accaaatgag cctccaatag caggtcttat aactcttact
    15661 ttatttaatg acatcccaaa aatttgagat agtattattc taaatccaaa tgtattttgg
    15721 caaggtgtat gtacagttaa tttatcatta taatcatagt gtgcaattac actatgagtc
    15781 tctattgcac tatgatgtat tgctggagta gagtattcat cttcaaatat ataatcacat
    15841 tccttgaagc ctttttctac atctccccca tttatgttgt tttgagaaac tatatttgaa
    15901 tctccatgta tgcagatagc atcttctttt attgcatctt gtatagtaaa tacagctggc
    15961 aattcttcat attcaacttt tataagtttt acagctttct ctgctatttc tggagtctct
    16021 gcagctactg ctgcaacacg gtcaccaaca aatctaactg tatctgaaaa tataacttca
    16081 ttttcaggta ttttgtgctc ataaaatctt aatgaaccat tgtattttct ttgtgatgta
    16141 ttgagataag tagctatagc tctaacacct actactttct cagcttcact tgtatctata
    16201 ctttttattt ttgcatgtgc tacagggctt aaaagcatct tagcgtaaag tacattttgt
    16261 ggtttcatat cagccacgta tttcatttga ccagtaactt ttaaagctgc atcttttaga
    16321 gggtatgatt ttcctattac cttttccata attatctacc ccctaatcta gctgcagata
    16381 attttacagc ttctataatc tttacatatc ctgtgcatct acagagattt tctttaaaag
    16441 cttctctaat ttcatcttca ttaggagatg catttctatt taaaagagct tgtgttgtta
    16501 taatcatacc aggagtacaa tatccacatt gtacagctcc gacttctata aaagcttctt
    16561 gtattggatg aagattttct ccattagcaa aactttctat agtgtatata actttacctt
    16621 cgcagttttt tgcaagaagt ttacaagatt tcatgtcaac tccatctatc aaaactttac
    16681 atgctccaca actgccacta ttacaacctt gtttcgctcc agttagattt aatttttcac
    16741 gaagtacatg caaaagagtt tcattctttt caacttctat ttcatacatg taatcattaa
    16801 tatttaattt tattttttcc attttctcct cctacagata aactaatgtt gtaatagaat
    16861 attattacaa tcttatttta atattagtat aagctattcc acttatgtcc ttccattcta
    16921 ttagttacat tagctctctt acataattct tctgctgcat gttgtgcatt aagcaacact
    16981 tgttcttcac tttcaattgt agttactttt ccattttcaa gtattacatt tccatcaact
    17041 aaaacactct ctacattgtg catactagct gagtatacca aagttgatac aggattgtga
    17101 agaggtattg attttggaga aagcattgag ttaaatatta ctaggtcagc ttttttccca
    17161 atctccaaag aacctatttc atcttccatt ctaagagttc ttgctccctc aattgtagcc
    17221 atttcaagaa ctttctctgc agaaattgcc agagggtcac aattatttac tttgtgttgt
    17281 aaagctgtta atttcataag ctctaccata tcttgtgcgt tattacttgc tgcaccgtca
    17341 agacctaaac tcacatttat accttttttt aacatttctg gcacaggtgc aacaccagaa
    17401 gcaagataca tgttacttgc tacattatga gaaactttta tgtcatactt tttaagcatt
    17461 tccatgtctt catcagttat acatacacaa tgtactgcta caacttctgg acctactata
    17521 ccccattttt caagtaattt tatatctatt tctccatgta tatcctttgt tgctgttctt
    17581 gcaaattctg tttcagatat atgaactgta aaatatgaat catattcttt aactatattc
    17641 caaagcatct tacccatttc ttcagatata gcccacattg aagaaggagc tacacagatt
    17701 tttattcttc cattttctga attatgatat ttttcgaata cccttctcaa atctttttca
    17761 accgtttcca catcttctat aagctctttg tgaataccta aatctataca acctctacct
    17821 acaacccctc ttatacctaa atctttatat gcctttacta ttccatctgt aagtcctggt
    17881 ctattgtgag tatgcatata atcaaccatt gtagttatac cactttttaa accttccata
    17941 catccaagca ttgcagcatc ataagtatct tgttctgtta agaattttgc tgatggaaac
    18001 atcattgtat ttaaccactc atgaagtgcc atatcatcac ctaaaccttt taataacact
    18061 tggaacaggt gattatgagt atttataaag ccagggaata ttacttttcc attagcatct
    18121 ataacttttt tagcatcaag atacttaggc tcaatatcaa ctgtatttcc tatgtccaag
    18181 attctgtcac cttgaatagc tattgctcca tcaaatataa catctctgtc tttattaact
    18241 gtaacaatta ttgcattttt tattagtata tcaatcatct taaatccccc tttgaatact
    18301 ttcattacaa cattttacca tatggttttc tcatattata tctaatacat ttatccaata
    18361 ttttcctcaa gatttgaagt aacgttttca gcacttttac gtgactttaa tttaaataat
    18421 gtttgaagta atatagctat tattgctcca gaaactatac ttgaccctcc aaggacagtt
    18481 ccaattgttt ctgggaattg gtctaaaact tgtggagcag ttgtaagacc catacttgtt
    18541 gctatagata cacctgctat aagattgttt tcttcagtga atccatccat actcataata
    18601 gagaaaccag acattgctat tgatgagaaa actactaaag ttgctccacc aacaacacaa
    18661 gatggtatag ttgtcattat tgcacccaat gctggagata tccctgctag taataataca
    18721 agtgccccta gtgctattac aaatctactt gtaactttgt tcatagatac aattactgta
    18781 ttttggctaa atactccagt tggaagagct gcaaatagtg atgaaattat agaagttata
    18841 gaatttccta aaacaactga tgctaattct tcgtcagtta cctctctatc cattgctcct
    18901 actgtagcta ttgtacaggc acccatcata tcagctatac ctattatgta aataatagta
    18961 aacattacta ttaactctgg tctaaattct aatccaaaag ctgctggtgt tggtaaagat
    19021 actatagctg ctccttgaac ggctgagaaa tcaaccaatc ctaaaactaa agatatagca
    19081 taacctacta atattcctat taatattgaa gaatttttta ctaaaccttt accgtattta
    19141 tttatcatta ttattacaaa agcaactata actcctactg tgaagtttat tgggcttcca
    19201 aatgtttgtg tgccttctcc tccagctaaa ttctttatag ctgttgcgaa tagtccaagt
    19261 cccatacatg ctattatagt tccagaaact attggagtga atatatgtct aattttctta
    19321 actccaaatc ctacaaagaa tacgattacc ccagcagcaa tctgtgctcc aaatagagct
    19381 gctaaaccat ctttagctgc tactgaaaga caagctccta aaaatacata actcattccc
    19441 atcattacag gtaatcttga acctaactta aatcctttaa ataaaggtat tggatataac
    19501 tgtaaaaacg ttgctaaacc tgctgctaac atagaaccct gaatcaacat tgttgcttgc
    19561 ccctggttaa gtcctaccac gtttgctatt aatatcgcag gtaccatatt tcctactatc
    19621 attgctaaca gatgttgtaa ggccaaaggt attgattcct ttagtggtgg tatcccatcg
    19681 acatcatatt ttgaagtatt acgtgttttc ataaaataaa tcctcccata cataaatatc
    19741 ttgactaaat atttttataa ttattttcta taaatatttt tatgaccatg aatttattgc
    19801 tcaaccgata aacacagcta aactgagcta tttttataaa aaattaacaa atttctaaat
    19861 attccctccc cgattttcaa cttaaatgaa cattaattgc ctggtatata tttatttgtt
    19921 aaatttttta ttaaaatgaa tagctatcaa aaataatcta acaatctcat actaaataat
    19981 ttaaacaatt cttttaaatt attatatctt aattagtctc ttccataaca gctctttctt
    20041 tagcctcatc ctctagactt ttatttggta ttattagatt taaaacgaat gcaaccaatg
    20101 ttgcaattac tattggagat tctccaaata ctaactttac aaatgaagga aatgcctctt
    20161 gggctgctgg tactgatgtt attcccatgc ctaaggcaat tgccaaacca acaatggtaa
    20221 tatttcttga tgacaactct tctgtaataa ttaactttat acctgtcatt gttatcatac
    20281 taaatataga tattgtagct cctcctaaaa cagaatatgg tatagtagtc attagtgcac
    20341 caaatttagg cacaaacccc gacaataaca taaatatacc agctattcca agcacaaatc
    20401 tacttattac tttgttcata gctaccatcc ccacgttttg gctatatgat gatggtggta
    20461 aacctccaaa caaagctcct atcatagtac aaattccact tcctattact ccacttgata
    20521 attccttatc tgtgatattc ctatccatgc ctcccattgt agtagctgat aaatctccaa
    20581 ttgcttgaac agcattgatt atacttacaa ttactactga gattattaca ggcatatcaa
    20641 attccatccc aaattcaagc ggtttaggta tcgcaaacca agttgcctct ctaatagggt
    20701 caaaatttat tatacccaga actaaagata cacaatatcc aactataatt cccaaaaata
    20761 ttgctgccaa ttttgcatat ccttttgcaa attgactaag acctataact accactaatg
    20821 ttatagccgc tactgcccag ttttgcatag aggcataagt aggactattt acaccacctg
    20881 ccatataatt tattgctata ggatacaaag ataatccaat tgtaaaaacc actgttcctg
    20941 caactatatt cggaaaaaat tttcttatct tttttatgaa tatccctatt aatactgtag
    21001 ccactccccc tattaattgt gcacccaaaa tgcttgctaa tccataattt cctccgactg
    21061 ctacaagtgt tggtacataa gtaaaaccaa ctccaaaaat tataggcaat cttgaaccaa
    21121 tatttcctat tggatatagt tgtataagtg ttgatatacc agctattatt aatgcatatt
    21181 gcactaaaag tgttatttgt gtagggctaa cacccacagc tcctcctacg actataggta
    21241 ctgtaacagt tccaactatc attgctagta tatgttgcat tgatagtggc aaacacttta
    21301 aaattggtgg ccttccatca atatcaaaca tagaaacatt cttttgatat ttactcaagg
    21361 ttatcactcc caactatttt ttaatgacag ttattattat ctcacacaac atcctttctt
    21421 tttttatttt gtcatctcaa cttaatatga agcaattata atgccaattt tttaacataa
    21481 ttgttttaca tatataattt ttattatatt tgttagtatt tgataattta ttaaagcatt
    21541 tattttttta aattctttta aatttttttt atttttggat aataaaatgc tattacacaa
    21601 aaaaacactt agaaaatagt tttccaagtg ttttttatca attttataat ttcaaatttt
    21661 agaattgaga ataaaaagcc taattattat acatctattt ttctgtacaa tgtagctagt
    21721 gatattccaa gttttttggc agctagtttt ttagacttag tatcatttcc gtaaaacttt
    21781 aacactttct ttatataggc tctttcaaac tcttcaagtg taagtatgtc ttgttccata
    21841 cttatctcat agtcactttc ctcaagtaac tttatattta tattatttaa cttaaaatat
    21901 tcaagtatct ttctatttat tattccttta tgtattattc catcttcctc aagaaggttt
    21961 aatgccagtt ctatagtatt ttccaactct ctaatatttc ctggccaagg atacttaagc
    22021 aacatttgtt ccacatctgg ctctatatgg tacacgtttt tcttcatctt tctcgaatat
    22081 ttgtctataa aattgtttat taatatttgt atatcgtctc ctctttctct aagtggaggc
    22141 acttctaaag gtattacatt caatctatag tataagtctt ctctaaattt attttcctta
    22201 accaactcaa ataaatctct attagtagct gatatgattc taacatctat atcaattagt
    22261 tcatttgaac ctattctcgt aaacttcttt tcctgtaata ccctcaataa ctttgattgt
    22321 agttgaattg gcatatctcc tatctcatct aaaaatacaa cccctccatt ggccaattca
    22381 aacttaccaa ttttaccttt tgaatctgct ccactaaaag ctccttttgt atatccaaaa
    22441 agttcacttt ctagtaaagg ttctggtata gcagcacagt ttatagcaac gaatgtttta
    22501 ttccctctgt ttcctgcgag atgaatggct cttgctatta attcttttcc agtgccactt
    22561 tctccagtta tagctacagt agaatcacta tttccaattt tacaaattct attcttaaga
    22621 gcacgcatta catcagactc tccaattatg gagtctactg ttgttgttcc ccaaatatta
    22681 tatacttcca ttccaccata cattactgaa tcactgtgtt tgtccataaa atatcaactc
    22741 caaataagta ttctatataa taatcattca cattatctat ttaattatat cataacagaa
    22801 aagcttagtt ataatttaat aatattattt taaaacaaaa cttaataaaa agtatatcta
    22861 cttagtttta atttgataga tatactttct tcattttaca aaaatcct
//
LOCUS       DAELTA010000048        22664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.048, whole genome shotgun sequence.
ACCESSION   DAELTA010000048 DAELTA010000000
VERSION     DAELTA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.048"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            31..495
                     /gene="bcp"
                     /locus_tag="KPG13_001621"
     CDS             31..495
                     /gene="bcp"
                     /locus_tag="KPG13_001621"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG0985354.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            505..1248
                     /gene="yaaA"
                     /locus_tag="KPG13_001622"
     CDS             505..1248
                     /gene="yaaA"
                     /locus_tag="KPG13_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG0985355.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1376..4033
                     /locus_tag="KPG13_001623"
     CDS             1376..4033
                     /locus_tag="KPG13_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG0985356.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4087..4189
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4299..5564
                     /locus_tag="KPG13_001624"
     CDS             4299..5564
                     /locus_tag="KPG13_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG0985357.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5594..6484
                     /gene="metA"
                     /locus_tag="KPG13_001625"
     CDS             5594..6484
                     /gene="metA"
                     /locus_tag="KPG13_001625"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG0985358.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6975..7418
                     /locus_tag="KPG13_001626"
     CDS             6975..7418
                     /locus_tag="KPG13_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0985359.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7449..9266
                     /gene="ftsH"
                     /locus_tag="KPG13_001627"
     CDS             7449..9266
                     /gene="ftsH"
                     /locus_tag="KPG13_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0985360.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9444..12146
                     /locus_tag="KPG13_001628"
     CDS             9444..12146
                     /locus_tag="KPG13_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG0985361.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12180..12878
                     /locus_tag="KPG13_001629"
     CDS             12180..12878
                     /locus_tag="KPG13_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985362.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13235..14191
                     /locus_tag="KPG13_001630"
     CDS             13235..14191
                     /locus_tag="KPG13_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG0985363.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            14368..14523
                     /locus_tag="KPG13_001631"
     CDS             14368..14523
                     /locus_tag="KPG13_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985364.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            14653..15666
                     /gene="aroF"
                     /locus_tag="KPG13_001632"
     CDS             14653..15666
                     /gene="aroF"
                     /locus_tag="KPG13_001632"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG0985365.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            15688..16752
                     /locus_tag="KPG13_001633"
     CDS             15688..16752
                     /locus_tag="KPG13_001633"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG0985366.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            16752..18065
                     /gene="aroA"
                     /locus_tag="KPG13_001634"
     CDS             16752..18065
                     /gene="aroA"
                     /locus_tag="KPG13_001634"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG0985367.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            18043..19122
                     /gene="aroC"
                     /locus_tag="KPG13_001635"
     CDS             18043..19122
                     /gene="aroC"
                     /locus_tag="KPG13_001635"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG0985368.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            19125..20321
                     /gene="pheA"
                     /locus_tag="KPG13_001636"
     CDS             19125..20321
                     /gene="pheA"
                     /locus_tag="KPG13_001636"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG0985369.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            20368..21177
                     /gene="aroE"
                     /locus_tag="KPG13_001637"
     CDS             20368..21177
                     /gene="aroE"
                     /locus_tag="KPG13_001637"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG0985370.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            21189..21722
                     /locus_tag="KPG13_001638"
     CDS             21189..21722
                     /locus_tag="KPG13_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG0985371.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            21743..22582
                     /locus_tag="KPG13_001639"
     CDS             21743..22582
                     /locus_tag="KPG13_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG0985372.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         9080 a         2426 c         4077 g         7081 t
ORIGIN      
        1 ttattaataa aaacaaaagg agagatattt atgttgagta taggaacaaa agcaccagaa
       61 tttacactag aagataaaga tggaaataag gtaagtatgt cagattttaa aggcaaaaaa
      121 gtagtagtat atttttatcc taaagataat acaccaggat gtacacgtca agcttgtgct
      181 tttagaaatg catatgatgg atttaaaaag gaagatatac aagttattgg gataagtaaa
      241 gatagtataa aatcacatca aaaatttgca gaaaagcatg aacttccttt tattttactt
      301 tctgaccctg acttagttgc tataaaagct tttgatgtat ggaaagagaa aaaaatgtat
      361 ggaaaaactg cattgggagt tgtacgtgcg acatatatta tagatgaaaa tggaattatt
      421 gaaaaagtct ttgaaaaagc aaaaccagac acaaatgcac aagagattct tgaatatttg
      481 gaaaaacagg agtaataaga atttatgatt ataatgttat caccagctaa aaatatgaag
      541 aatatagaag tatttgatag agatttgagt ttgccttgct ttatagataa cacaaaagaa
      601 atagtggaaa atataaagac atttggtata gaagatttta aaaataaaat gaaaattaat
      661 gaaaaattag ctgtattaaa taaaaataga tttgaaagca ttaaatttga tagattagga
      721 aaccctgcaa tcttaactta tgatggaata caatataaaa atatagaagc agaaaatttt
      781 acaaggaaag atgaagagtt tgcaaatagc tgtattcgaa tcataagtgg attgtatggt
      841 gttgtaaaac catatgatag tatatatgaa tatagattag aaatgcagac aaaattacaa
      901 gtaggtgagt ttaagaattt atacgaatat tggggaaatc gtatatataa agaactaatc
      961 aaagaaaaaa cagctatcgt taatctttca tcaaatgaat acagcaaatc catagaaaaa
     1021 tttattaaag atagtgatac atatattaca tgtactttta aagtaaataa aaatggcata
     1081 ttgaaagtgg aatctacaca agcaaagaaa gctagaggta tgatgacaaa atacattgta
     1141 aaaaatagga taagagatat tgaagaactg aaaaagtttg atttagaagg ttataagtat
     1201 aaagaaaact tatctaacaa tagtgaatac atatttgtaa aagagtagaa gttttgttaa
     1261 atattaaatg gcattgttaa atttaggtaa aatatactta gtgtgtattg aattgaactt
     1321 gattttaaaa tataattatt cttaaattaa aaaaagaaag gagtggtctt ccaaaatgga
     1381 aaatcacaaa cagatgcctt ggcattcaag agcaacgcat gaaattttag aaagtctaga
     1441 ttcatcagag gaggggctca ccgataccga ggcggaaaaa cggcttaaag agaatggctt
     1501 aaatgaactt agaaaaaaac cacctagaac aactttacaa atgctttgga gtcaaattac
     1561 agatgctatg gttatgatac taataggagc agcaatatta tctttgatat ttggcgaatt
     1621 tacagaagct tttgtaatac taataattgt aacagtaaat gcagttattg gaattgttca
     1681 agagaaaaaa gcagaatcat cattagaagc attaaaaaat atgaattcac ctacagctcg
     1741 tgttatgcgt gatggtgaag aaaatgttat tccagcaagt aatcttgtag taggagatat
     1801 tgttttttta gaagatggag ctatggttcc agcagacctt cgtttaatag aaacatcaag
     1861 tttaaaaatt caagaggctt ctttaacagg agaatctgtt ccatcagaaa aagatgcaga
     1921 tacaatctta cctaaagaat gtgtattagg agaccgtagc aatatggcgt atacatcttc
     1981 aatagtcaca tatggtagag gtgtaggtgt tgtagtagca actggtatgg atacagaagt
     2041 gggtaacata gcacatcttt tagattcaca agatgatttt gatactccta taaaacgtaa
     2101 acttaatact gttggaaaga cattaagtgt cataggtatt attgtatgcg ttgttatatt
     2161 tgcaataggt gcattttatc agagacctct cttgccaatg tttatgattg caatttccct
     2221 tgccatatca attattccag agggattacc tgcaacagct actattgtaa tggctttagg
     2281 cgtacagcgt atggctaaaa gaaatgcttt aattagaaaa ttacctgctg ttgaaacatt
     2341 aggaggagct acagtaatat gttgtgataa aactggtaca ttaaccttaa ataaaatgac
     2401 tgttacacat attgctgtca atggagattt tgaatctgga aaagtgacat caatagatac
     2461 tgcatctgaa aaacatccat tagtgtataa agagattgtt tatgctggtg cactttgtaa
     2521 tgatgcatgt attaatccag ataaaacaga tgaaatatta ggtgacccaa cagagggcgc
     2581 attaattttt atggccaaaa aatttggaat agatcaagaa gctcttgaag aagaatatcc
     2641 acgtgtattt gaacagcctt ttgattcaga tagaaaatgt atgacaactg ttcataatat
     2701 agatgaaaag attattgctt atactaaagg agcagtagac gaaattcttg acctatgtac
     2761 taaaatatta acttcaaaag gtgaaagaaa cattactgaa gtggataaaa agaatataca
     2821 cgaattatgt cttaacatgt ctaaagatgc attgcgtgta cttggttttg ctaagcgtga
     2881 gatttcatct ataccaaaag aagatagtga aaatatagaa tatgatttaa catttatagg
     2941 tatggttggt atgattgacc caccaagaac tgaggtaatt gatgcagtaa gtacttgtaa
     3001 agaagcaggt ataagaacca taatgataac aggtgaccat aaagttactg ccacaacaat
     3061 cgctcatgag ttaggaatat ggagtgaaga aaatactgtg atatctggtg atgaacttga
     3121 taatttgagt gatgatgaac tagatgaagc tgttaaaaat actacagtat ttgctcgtgt
     3181 atccccattt gataaactta gaattataca atctctaaaa agaattggag aagtaccagc
     3241 aatgactggg gatggtgtaa atgactctcc tgccttaaag tctgctgata ttggtatagc
     3301 catgggtata agtggtacag atgttgcaaa agattcttct gacatgattt taatggacga
     3361 tagttttaca acaattgctt atgcaataaa agaaggtaga cgtgtatata gaaacataca
     3421 aaaagttata caatttttgc ttgttggtaa tatagcagag atacttacgt tgtttgtagc
     3481 aatattatta aattgggata ctccgcttct tgctgttcat attctatggg taaaccttgc
     3541 aactgctacg cttccagccc ttgctcttgg agttgaccca ccaagcagaa atataatgaa
     3601 gcaccagcca gtgaaatcaa ccactttgtt tgaaaaaaat cttatacaaa gagttgttac
     3661 ccaaggtata tttgtggcaa taatgactat tttagcatat aaaattggtc ttaatataga
     3721 aaatcattct gtaggtcaaa caatggcatt ttgtgtatta gcattttcac agatgttacg
     3781 agcatttagt cagcgttcaa atacagattc aatgtttaat agaaataatg gtaaaaatcc
     3841 atttctttta atctcatttt taacttctgc atttttaata gcagtaatac tatttgtacc
     3901 tgtttttaga aatgctttta acttaacagt tttaaataac ttagagtggc ttgtaacgat
     3961 tgttttagcc ataatgtctg taattcaagt agaggttggg aaaattatta aaagaaaaaa
     4021 attaaaaaaa tagttgacaa aattaggaaa aatatataag atataaaaca tagaaaaatt
     4081 aaataaatct tatccagaga ggtggaggga ctggccctat gaaacccggc aaccagtata
     4141 tttatataca ttggtgctaa atcctgcggc gaaagctgag agatgagtat aattaagctt
     4201 atacagagcc tgagttatat actcaggctt tatttatttt aactctcatc tggtaaatag
     4261 tttaattttt tttactaaaa tctaaggggg atacaagaat gtataataaa gaaacaatat
     4321 gtgtgcaagg aaattataaa ccaggtaatg gagaaccaag agtactacct ttatatcaaa
     4381 gtacaacttt taaatatagc agtatagacc aacttgctga attatttgat ttaaaagttg
     4441 atggacatat atattcaaga ataagcaatc ctactattca agcttttgaa gaaaaaataa
     4501 gtttactaga gggtggagta tcttctgtag ctgtatcatc agggcaatct gcaaatatgt
     4561 tggcagtttt aaatatatgt aaatcaggag atagtatact ttgttcttca aaagtatatg
     4621 gaggaacatt caatttacta ggacctagtc ttaaaaaatt tggtatagat ttaatatcgt
     4681 ttgacttaga ttcaagtgaa gatgagatag tagaacttgc aaaggaaaat actaaggttg
     4741 tgtttgcaga aacacttgca aatccaactc ttgaagtcat agattttgaa aaaatagcaa
     4801 atgtagctaa gagaattaat gttccattta ttgttgataa ttcattagca tctccagtgc
     4861 tttgtaaccc tttaaagtat ggagcaaata tagttactca ttctaccaca aaatatttag
     4921 atgggcatgc ttcaagtgtt ggaggaatta tagtggatgg tggaaacttt aactgggata
     4981 atggaaaatt tccagaatta gttgagccag acccaacata tcatggtata agctatactc
     5041 aaaaatttgg aaatgccgca tatgcaacta aagcaagagt tcagttgctt agagactatg
     5101 gaaattgttt aagcccattc aatgcgtatc ttactaattt aaatgttgaa acactacatc
     5161 ttagaatgga gagacatagt gaaaatgcac ttaaaatagc tagattttta gaaaaacatg
     5221 aaaatgtaga ttggattaat tacccaggac ttgaagataa caagtattat gagaatgcca
     5281 aaaagtattt atcaagagga tgtagtggtg ttttatcatt tggagtaaga ggtgggttag
     5341 aaaatgccaa aaaatttgtg gaaaaattac agatagcatc tttggttaca catgtttcag
     5401 atgtaagaac ttgtgttata catccagctt caactactca tagacaatta acagaagaac
     5461 aattaattgc atctggagta ttgccttcac taataagatt atctgttgga atagaaaatg
     5521 tagaggattt aatagctgat ttaaatcaag ctttaaattt ctaatattct aagagaaaga
     5581 gaagtgaaaa gatatggctt taatactacc aaagggatta cctgttatta ataaattatt
     5641 agatgaagga atagatgtaa tttataaaga agattttaaa aaggaattaa agtatgaaga
     5701 aaacatagac actaagatag ctatactaaa tctaatgcct ataaagatag atacagaatt
     5761 agatttacta agaaggatag ataaaacagg attcaacgtg tctgtggaat ttattaaaat
     5821 ttcaacaaga gaaagtaaaa gaagttgtaa tgaatatgtt aaagaatttt ataaaacatt
     5881 tgatgaagcc aaaggagagt attttgatgg ctttataata acaggagctc cagttgaaca
     5941 aatggagttt gaagaagttg attattggaa tgaactagaa gaaattatgg attattctaa
     6001 gagaaaaact aaatctacat tatatatatg ttgggcagct caagcaagtc tttacaaata
     6061 ctacaatgta aaaaaacttc cactaagtca aaaatgtttt ggagtattta agcataaggt
     6121 agataaagat agtaaaatag tagatggatt tgaaaatgaa ttttttgctc cacattcaag
     6181 acatactact gtaaatattg aagctttgaa agagaataaa gagttgagca ttgtgagtca
     6241 ttctaaagaa gctggtccct atataattac aaattcaaga gatgtgttcg taatgggtca
     6301 tagcgagtat gacaaataca ctcttgataa agaatataag agggatataa ataaaggaga
     6361 taaaatatct atacctcaaa attactatat taatgatgac ccttcagaag agccaactgt
     6421 aaaatggaaa aaacattcag aattgttgtt tagaaattgg ataaaaaact atttgataca
     6481 ataaatttta agagtatata aatgttaact aaaaaaatat agaaatttat aggtataaag
     6541 ttaatcttaa tgatacttta taccttattt ttttaaaatt tgaccttata atagtagttt
     6601 tatattttaa gaaaatcact ttaggtgtat gtagatagag gtggctttaa ttttatatat
     6661 ggtgcgataa tgggagacta aacctttata gtatcaataa atttgtattt taacttttaa
     6721 aagagattaa aaagggagaa atcagtgtaa aatatatata tagatttttt attaaatttt
     6781 attaaaaata tcctgtataa aattaaatat caataagata acaaaatttc aattgaagtt
     6841 acaatattgt aattgacaat aaatacttaa tactataata tttaatacat taaacattat
     6901 agtgttaagt atttatttac agtatgcact tcagcctaaa tttgaaataa agtattttcg
     6961 tagggagcgt ttgtatggat gagatgagtt ttaagaaaga gctactagag ttgactagag
     7021 atataaatat gaaatttaca actttattaa gtgattttta tcagccactt ggtattacag
     7081 cagtgcaagc tttgatttta tctgagttat gtgaacatgg agagaaaaaa ataagcgatt
     7141 tagggaagaa cttaaacatg acaaatagta atgtatcagt aatctgtcaa aggcttgaaa
     7201 aaaatggctt tttaaatcgc ataagagata tagaagacca acgaattgta aaagtaaaag
     7261 ttacaaataa atcattggat atacaagaac atatttcaag tagtattttt gatagttatt
     7321 ttgaaaatat gaccagtgaa aaattgcaag atatggaaga tataatagaa ggtttagaga
     7381 aattaaataa acttttaact tacattgatt gcaaataaca taaaaataat ttaaaggaga
     7441 ataaaattat ggatgaacat aaaccccaaa aaaggtcagc actctattat tatacagtta
     7501 taataatagt agtaatgttt ttaaatgctt taatatttcc aagtttaatg gataaaaaaa
     7561 tggttgagat tgactatgga aagtttctca caatgttgga tacaggaaag gttaaatctg
     7621 ttgagataga aaataataga atagcaataa atccatctag tagcaaggat aagaatatat
     7681 acataactgg tagaatggat gacccagaat tggtagatag actaaaaaat gcaaaagtag
     7741 aatttactaa ggttattcct aaggaaaatt cacctctttt aagtatttta cttacatgga
     7801 taatacctat aggtattctt atgctatttg gtaacttaat gatgaagtct atgcaaaagc
     7861 gaatgggtgg taatgctatg caatttggaa aaagcaacgc caaaatgtat gtatctgctc
     7921 aaagtggaaa aagatttaat gatgtagctg gtcaagatga ggcaaaagaa gctttaactg
     7981 aaatagtaga tttcttacac aatccagaaa agtataaaaa gataggagca caaatgccta
     8041 aaggagcact tttagtagga cctccaggta caggaaaaac actccttgca aaagctgtag
     8101 caggtgaagc aaatgtacca tttttctcaa tatctggttc tgagtttgtt gagatgtttg
     8161 taggaatggg agcttctagg attcgtgatt tatttaaaca agcaaaagaa aaagctccat
     8221 gtatagtgtt tatagatgag attgatacta ttggtaaaaa gcgtgataat ggaagtggat
     8281 taggtggaaa tgatgaacgt gaacaaactt taaatcagtt gttaagtgag atggatggtt
     8341 ttgatggtgg aattggagta gtaatacttg ctgctacaaa tagacctgac agtcttgaca
     8401 aagcattact tcgtccagga agatttgata gacgtatacc agttgaactt ccagaccttg
     8461 gaggacgtga agctatatta aaggtacatg ctcaaaatgt aaatgtaagc tcagatatag
     8521 attataatgc aattgcaagg tctacatcag gagcatcagg tgcagaactt gcaaatattg
     8581 taaacgaagc agcactttta gcagttaagt ctggcagaga tatagtagaa caacaggatt
     8641 ttgatgaatc tgttgaaact gtaattgctg ggtatcaacg taaaggagct gtaatttcag
     8701 ataaggaaaa gaaaattata gcatatcatg aaataggtca tgcacttgtt gctgctatgc
     8761 aaaaacattc tgcacctgtc cataagatta ctataatacc tagaacttca ggagcattgg
     8821 gatatactat gcaagttgct gaagatgaga gtgtacttat gtctaaagaa gaagcaattg
     8881 ataaaataac tacttttaca ggtggtcgtg ctgctgaaga ggttatcttt aacacttgta
     8941 catcaggagc atcaaatgat atagaacaag ctactaaaat tgcaagagct atggttactc
     9001 gccttggtat gagtgaagag tttgatatgg tcgctcttga aactgtaagc aatcaatatt
     9061 taggaggaga tacttctctt gcttgttctt ccgacactgc tgctaaaata gatgaagagg
     9121 tatttaaaat agtaaaggct tgtcacaaaa atgcaattga aatcttagaa aataatattg
     9181 ataaattgca tgaacttgct aattatttat tagaaaaaga aactattaca ggtgatgagt
     9241 ttatggaaat acttaactta aactaaaatg attatacaca agataaaaat gcctattgtt
     9301 aggcattttt attttacaac taaatttttt aaatttatat ttaaatcttt acataatctt
     9361 aatcattaaa aatacatctt aactacaatt ttatatcgga attgatatta taatagtaat
     9421 gaatggtgta aggaggtttt ttaatggata gacctaatcc tgatattcta ttagaaaaaa
     9481 ttaaaaatga agaagaaaaa ctaagtagag gtcaattaaa aatattcttt ggttatgcag
     9541 ctggagtagg aaaaacctac tctatgttgg aatctgcaca aaatttaaaa aaagtgggtg
     9601 tagatgtagt agttggatat attgaaccac acactagacc agaaacattg gctttacttg
     9661 atggcttgga aattcttcct gtaaaggaga tagaatataa atctataaaa ctaaaagaat
     9721 ttgatttaga tgctgcttta gcaagaaaac cagaagttat attagtagat gaatttgccc
     9781 attctaatgt tgctgggtta aggcatacaa aaagatggca agatatagaa gaattgctgt
     9841 tagcaggcat aaatgtctac actactgtaa atgtacaaca tttagaaagt ctaaatgaca
     9901 ttgttgagat aattacaaac gtatcagtta aagagactat accagataag tttttggata
     9961 caaatacaca gttagaactt atagatgttg aacctgatgt acttcttgaa aggttcaatg
    10021 aaggtaagat atataagaaa gaacaagcta ctagagcaaa aaataatttt tttataaaag
    10081 ataacctggt tgcacttaga gaaattgcac ttagaaaaac agcagagaga gttaataaag
    10141 aagttcaaat gaccagacta tcaaagggtg atgtaactgt aatacctaca tcagatacac
    10201 ttcttgcatg tataagtcca tctccatctt cagcaaaagt tattagaact gcatccagaa
    10261 tatcagacag tacatttgca aaatggatag ctttatatgt tgaaactcca aacacagcaa
    10321 gacttccatt tgatgaacaa aaacaactac aaaataacct aaaattagct aagaaattag
    10381 gtggagaaat aattgtttta catggtgaaa atataataga gcaaatactt agaattgcaa
    10441 agctgagaaa tgtaacaaaa attgtaatag gtagaaatca ttcaagtaat aaaagattta
    10501 gtaaaagatt taaaaaagat atagttgaca aattaattga tgaagttgat tatatagata
    10561 tacatataat tccatataaa tttgcctcag atgttaaata caaacctaaa aaggataaat
    10621 catcaataaa atctaaattt aaaattagca aagttgattt tataaaactt atatttatta
    10681 ctttgatgat aagtatttta gcatatgcag tacaaagcat gggatttata agagaaaata
    10741 tattgttaat atatatgttg ggtgtagttt tagtttctat gtggactaaa gggtatagta
    10801 caggtataat aagttctgta tttaatatag ttcttttaaa ttattttttt acagcaccat
    10861 tatatacatt atctatagca gattcaaact atatagtaac actggttgta ttctcaatag
    10921 taggtataat aactagcact cttacgtcta agatacagca tgaagcagaa actgctgcta
    10981 aaagagaaga aaatacaaaa atgatatatc aaataattaa gggattttta agattatcaa
    11041 ataaagatga tatagtaaac aaaggtatag aacttttgtc tcttagctta agtagagatg
    11101 taatttgtta tttaaatgta gataagcata ataaatcaaa gctttacaaa aaaaatacta
    11161 ataataagga taaaaatgat ttggatagtg aagatgaaaa agctgttgca agttgggttt
    11221 tatccaattc aacagtagca ggaaatgaca cggatacact tcctggttca aagggatact
    11281 atataccaat tgtaggaatg aatttaactt taggagtagt gggaatatca tgtattgatt
    11341 caaaattgga tacagaagat ataagcttaa ttgaaacaat aattgctcaa atggcaatag
    11401 cgcttgatag agaaatcttg tcagaagcta aagaaaatac caatcttgag attgaaaggg
    11461 aaagattaag aagtaatctt ttaagagcag tttcgcatga ccttagaact ccactagcag
    11521 gaatatctgg tgcagtaagt acaattataa aaaacaaggg tactataggt caagatataa
    11581 tagatgaatt acttagtggt gtatatgaag atactcagtg gttaataaga cttgttgaaa
    11641 atttacttag tatgaccaag atagatgaag ggaaattgga agttaaaaaa cattctgaac
    11701 tggttgagga aattatatca gaagcgttac aaaaaataaa aaaaagaatt gaaaatgcac
    11761 ttatagatat taatatacca gaacagatat tatttgttcc tatggatgca aaattaatag
    11821 aacaagtttt aattaattta atagataatt ctttgaaata ttcaaaggaa gattgtaaga
    11881 tagagataaa tgtatatgag aaagatgatt atgtttggtt tgaagtatct gataatggtc
    11941 ctggtatatc aaaggaactt aaaaagcata tctttgacag attttttact ggagaagaag
    12001 gagctaaaga ttccagaaag ggtgttggac ttggattatc aatttgtaaa tctataattc
    12061 aagctcataa aggtgaaatt atggtagaaa acaataaaga taagggttca acttttaagt
    12121 ttagtttgcc taaggaaaat gaatagtttt aaattttaaa attagttttg gaggaagaaa
    12181 tgagtaaaaa acttatattg ttagtagagg atgataagac aataagaaaa tttatatcta
    12241 ctgcactttt aacacaggat tatgatgtca aggaggctat aactggaaaa gaaggtatat
    12301 ctatagctgt ctcatattct ccagatgttg ttcttttaga tttaggctta gaagatatgg
    12361 atggaataga ggtaataaaa gctatcagac aattttcaaa tataccaata attgtagttt
    12421 cagctagaga gcaagataga gacaaggtag aagtatttga tgcaggggca gatgattact
    12481 taacaaaacc attctccata gtagagttat tagcaagagt aagagtagca tttagacatt
    12541 cacaagtaga agctcaacaa aaagatgatg ttaaatctac gtttgaagta gataaattat
    12601 tgattgattt tgataaaaga aaagttattg ttgatgatgt tgaagtccat ttgacaccta
    12661 ttgaatataa tattctttca ttacttgcaa agtatcatgg aaaagtgctt actcataatt
    12721 ttataataaa agaaatatgg ggtagtgtta tagggaatga gactaagtct ttaagggtct
    12781 ttatggcaac gttaagacga aaaatagaaa aacaacctgc aaacccaaga tatataatta
    12841 cagaagttgg tgtaggatac agattaaatg atgaatagtt gtaatatatt aattattaga
    12901 ttatgtgctt attaatatat attaaaatta tatagtttag ttaaaataag aatgaagtgt
    12961 atgtgcttgt tgtcagttta gcacatgcat tttatttttt agctttttta gtatcaataa
    13021 ttttttagta tcaataatat gaaatatgtt ttaaaataag tatatagtgt aatatgtaat
    13081 aaaaactttg gttaaataat aaatgtattt aacacttacc ttataataaa tttaatataa
    13141 atattaaata ataattatta aaatagtgta aaataattac agtaaggatt ataaaaaatt
    13201 tttagaatgt aaattgaaat ttaggagtag atgtatgtat atactgaatt taaatacaag
    13261 agagactatt gaagatttta gagataaatt ttatgtagca gaaaattctt atcttatatt
    13321 aagtgctcca aaaaatttaa aattgcttaa ggaaactttg gatatagatg aaataacttt
    13381 taatgattgt cttaaatttg atgaaataac caaacttgat ttatttgata attacgattt
    13441 tttaagccta aatacatttg aactaagaga tggagaagct gttatagaag aggtaaatat
    13501 gtatttatca gataatttta tattggttgt agtgaatgaa gaacactttt tatttgaatt
    13561 tgtaaaaaat attattttga agaactctca acttgaaaaa aatccagtta ttaatctatt
    13621 taagataaac tacttgattc ttagagaagt tataaaaaat ggttttgagt ctttggaaaa
    13681 agttgaagag ttaattcttc aaatagaaga tgaaatgatg gacaacatta ataagaatca
    13741 tgtatctaga ataagtgatg taagaggatt aacaagaatt atagttaaga atactagacc
    13801 tcttttgtat attggggata gaatagttaa ggaaaatata agatatttaa aatattcaaa
    13861 tgtaaaaaaa tataatttag aaaattttca aggtatagat tttggaattg acaaattata
    13921 caatttcgca ttgtcaacac gagagttagc agataaatta ttagatattt attcttccag
    13981 agtaggtgag aaaacaaata acttaattac aaaattaacc ttgttaacag ccatatcagc
    14041 accacttaca attataactg gtatatatgg aatgaacttt agatatatgc cagaattaaa
    14101 ctggatttat ggatatccag caactttatt ttttatgcta tgtataattt ttgttggaat
    14161 tataatattt aaaataaaaa aattattgta aattacagat gatgtttaat tgattttata
    14221 aaaatacatc aattgagaag ctgatttaag taaataaaaa aatattttaa attgaaaata
    14281 tttatagaaa atattgacaa ttatattttc tttgttataa ttattaaaca agaagaataa
    14341 taaattataa aaatggagga taatactatg aaaggaatat taaaagaact aacagcaaaa
    14401 ctttatagct ttttttactt tatgttctgg ggcttttatt ttagcgctgg ctatttcttt
    14461 tacaaaaata tatgtacttc ctttctggtg aggccttatt ttagtgattt tgagcataag
    14521 taaattttat aaaaatgatt attttatata tttatataag atttagtgct tgatagatta
    14581 ttttagagaa ctcattagtg agttctcttt tttattttaa atataaaatt ataatattgg
    14641 gaggtagtaa aaatgatagt agtattaaaa atgggagcag ataaaaatga agttaaaaaa
    14701 ttaattgagg ctattgggag agaaggagta gaagttaatc ccatagatgg aacagaatta
    14761 actgttttag gtttagttgg agatactagt aaaatagatg ccaaaagaat agaagcaaat
    14821 aaggtagttg aaaaagttat gcatgtggta gagcctttta aaaaggccaa tagaaaattt
    14881 catccagaac cttcaatcat aaatgttaat ggaatggaaa ttggttcaaa gaaaattgct
    14941 atgatagctg ggccttgttc tgttgaaaca gaagaccaaa tagtatctat agctaaagat
    15001 gtgaaaaaat caggtgcagg atttttgaga ggtggagcat tcaaaccaag aacatcacca
    15061 tacgctttcc aaggattgaa atatgatgga ttggatttat taaaaaaagc aaaagaaaaa
    15121 acagggcttc ctatagttac agaaataatg tcaacacaag atatagatat atttgaagaa
    15181 aatgtagatg taattcaagt tggagctaga aacatgcaaa actttgattt attaaaagaa
    15241 ttaggtaaaa caaataaaac aatactttta aaaagaggat tatctgctac aattgaagag
    15301 tggctaatgt cagcagagta tataatggct ggaggaaatg aaaatgtagt tctttgtgaa
    15361 agaggtataa gaacttttga aacatatact agaaatactt tggatttaag tgcaatttta
    15421 gctgtgaaga aacttagtca tttaccagtt atagttgacc caagtcatgc agcaggtaaa
    15481 tcatggatgg ttgactcttt atctaaggca gcaattgcag ttggagcaga tggtctaata
    15541 atagaagttc ataatgaccc agcacatgca ctttgtgatg gaaaacaatc gattaagcca
    15601 aatgaatatg atgaattgat aagtgaatta aagactatcg catcagcagt tggtagagaa
    15661 atataatcta taaaaggatg gttaaacgtg gaagaaaaac tattagtaaa tttaaaagaa
    15721 aatagctaca atatatttat taaaaaaaat atactaaaga atataggaga agaaataaaa
    15781 aaaatatatt ttggaaataa gatttttatt gtaactgatg aaaatatagc aaaattatac
    15841 ttggataaag taaaaaataa tttaagtgaa tctggatttg aaattaaatc tttaatatta
    15901 aaatctggtg aagaaacaaa atcatttaat actcttccta aaatatacga tagtttactt
    15961 gactttaagt taactagaaa agatttgatt attactttag gaggaggggt aataggtgat
    16021 ttaggtggat ttgcagcagc aactttttta agaggtataa gttttattca aattccaaca
    16081 tctttattag cacaagttga tagttctgta ggtggaaaag ttggagttga ccttgagaga
    16141 ggcaaaaact tagtgggaag cttttaccaa ccaaaggctg tgtttatcga cccaaatgta
    16201 ttattatcac ttccagaaaa attctataga gatggaatgg cagaagttat aaaatatgga
    16261 tgtataaaag acaaaacttt tttcaataaa cttttaagtt taaaaaatag agaagaagta
    16321 atgcaacata tagaatacat tatacataaa tgttgcttta ttaaaaaaac tgtagttgaa
    16381 gctgatgaaa aagactttgg agatagaatg cttctaaact ttggccatac attaggtcat
    16441 gctattgaga aatactacaa ttttacaggt tatacccatg gagaagctgt tgccatagga
    16501 atgtataaga taactttaga aagtgaaaag ataggaatta caaaaaaagg tacatcagaa
    16561 cttataaaat caattttaat gaattataat ttgccttatg agattgaatt aagagataat
    16621 aaatctatag tagatgctat atctcttgat aaaaaaaatc taggtgatac attaaaaata
    16681 atacttttaa aagatattgg agagagtatt atatataata ctacttctaa attttttcag
    16741 gaggtaagat aatgggaagt ctaaaaatat atccaagtaa attatctgga gatgtaaaaa
    16801 taccaccatc aaaaagtatg gcacataggg cagttatttg ttcatctcta agcaatggaa
    16861 aaagtagaat ttctaatata gatttttctg atgatataat agctactata agagcaatga
    16921 catcattagg agctattata gaaaagaaag aagatatttt agaaatttcg ggaatttttt
    16981 ctaaagaagg tattttaaat agagagaatc agttgaatca acctaaattg acaatagatt
    17041 gtaatgaatc aggctctaca ttgagatttt tagtacctat atctttaaca tttgatggtg
    17101 taaaaaggtt tataggtaga ggtaatcttg gaaaaagacc acttgatacc tattatgaga
    17161 tatttgacag acagaatata aaatatagtt acaaagaaaa tcagttagat ttgattatat
    17221 caggtaagct taaaccagat gaatttagag taaaaggaaa tataagttct cagtttatca
    17281 caggtttact gtttatattg cctacgctag aaagtgattc aaagataata attactactg
    17341 agctagaatc taaaggatat ttagatttaa ctctttcaac gataaaagat tttggggtag
    17401 aaattataaa caataactat aaagaattta ttataaaagg aaatcaaacg tataaagcga
    17461 gagattataa agtagaagga gattattctc aaggagcatt ttatttatca gcagatgcta
    17521 taggagaaga tattagcata ttagacctta aagaagattc tcttcaaggg gatagtgaag
    17581 tagttgaaat tttatctagg atgggaatgg aaatattaag agaaggcaac aaaataaaag
    17641 gaataacaaa tggtttaaat ggtactttga tagatgcatc tcaatgccca gatataatac
    17701 ctgtattatc tgtagtagca tccttaagta tagggaggac tacaataatt aatgctggta
    17761 ggcttagaat taaagaatgt gatagacttc atgcaataaa tgttgagtta agtaaacttg
    17821 gagcaaatat agaggagaaa gaggatagtt tgataatcga aggagtaagc aagttaaatg
    17881 gtggagtaga ggtgtggagt cacaaagacc atagaattgc tatgacactt gcaattgctt
    17941 cttgtagatg tgataaacca ataatattaa aagattttga gtgtgtatca aaatcttatc
    18001 cacatttttt taaagatttt aagatgttag gaggtcgaat tgatgagtgg aatatgggga
    18061 aataatttaa aagtatcaat ttttggagaa tctcatggaa atgctatagg tataaatata
    18121 gatggtcttc cttcggggat tgaacttgac cttgataata ttgataaaga aatgaagaga
    18181 agagcacctg gaaaaaattc tatatctact tcaagaaatg aaagtgatat accagaaatt
    18241 ctaagtggat attttaatgg aagaactaca ggaacgccat tatgtgcaat cataagaaat
    18301 agtgatactc gttcaaaaga ttatggagag cttaaaaatt taatgagacc aggacatgca
    18361 gatttcacag gtaatgtgag atattctgga tttaatgact atagaggggg aggtcatttt
    18421 tcaggtagga taacagctcc attagtattt tgtggggcta tatgtaaaca aattttatca
    18481 caaaaaggaa tagagatagg agcacacata aaaaaaatta aaaatataga agatatgagt
    18541 tttgattatg taaatatttc aaaacaacag ttatcaaatt tgcaaacttt ggaactacct
    18601 ttacttgatt tatcaaagga agaagccatg aaaaatacga ttatagatgc aaaaaatcaa
    18661 ggagattcag ttggtggtat aattgagtgt gcagtagtag gtattaatgt agggcttgga
    18721 aatccatttt ttgactctgt tgaatctact ttatcacatt tattgttttc tgttcctgct
    18781 gtaaaaggtg tagagtttgg tttaggtttt gaacttgcag atatgtatgg gtctcaaagt
    18841 aatgatgaaa tgtattatga aggaaatcaa gttaagagca aaacaaataa caatggagga
    18901 attataggag ggattactac tggaatgcca ataattttca aagtggcaat taagccaact
    18961 ccatcaatta gcagacaaca aaacacagtt aatataaaag ataaaaagga cgatatttta
    19021 tatattaaag gtagacatga cccttgtata gttcaaaggg ctatacctgt aatagaagca
    19081 gtaacagcca ttggaatttt tgatttgatg aaaggacgat aataatggag gatttaacga
    19141 gatatagaaa tgagattgac aatatagata aagagcttat tcaattattt gaaaagagaa
    19201 tgaatacagt tcttgaaatt gccagacata aaatgaaaaa taacactact atacttcaaa
    19261 aagatagaga agaaaaagta ctaagtagag ctgtagataa tttagataat aaagcatatt
    19321 cacaagaaac ggtacagttt tttaattcta taatggaaat tagtagagga ttacaaaaaa
    19381 gattgataga taatgatggt gagcagaaaa tcgagtttag tttagattca tcaaataaga
    19441 aatttgattt gtcaaatgta aatagatata agtctttaaa agatgaatta aataaaaaaa
    19501 atatcctagt aggatttcca ggaaaatcag gttcatttac agaagaagca ctaaataaat
    19561 tcttcaacaa aaaaatatca aaaaaacaat ttaaagaatt tgaagatgtt ttcatagcac
    19621 ttaaaaataa agaaataaac tatggaataa tccccataga aaattcatct acaggagcta
    19681 tctctgaaac ttatgacctt ttgagaaagt acggttttta tatagttgga gaagaatgta
    19741 taaaaataga ccaaaattta atagggataa aagacacaaa gcttgaagat ataaaagaaa
    19801 tatattctca tccacaaggt ataggacaat gtagtaaatt tttaaaacaa aatagtacat
    19861 ggaaattgat accatttaat aacacagcaa caagtgcaga acttgtaaga aaacttcaag
    19921 ataaaacaaa agcagcaatt gcaagcaaaa aagctgcaag tatttatgga ttagaaataa
    19981 taagtccttg tataaatgat attgcaaaca actatacaag atttgtagta atcagtaatc
    20041 aaatacacat tgaagaagaa tcaaacaaaa tgagtgtagt attttctgtt gaacatgaag
    20101 caggaaaact ttataaagta cttggatact ttgctaaaaa taatataaat atgacaaaaa
    20161 tagaatcaag acctatgaaa aatgcctcat ggagatattt cttttatata gattttgagt
    20221 gttctatata caatgctaaa gtgtacgatt tattagaatt gatagaacat aatacagcat
    20281 attttaaatt tatgggagta tataaaaata aaactgaata gataagataa atttttattt
    20341 aatttataat tattaggagg tgtaagtatg aattttttcg gtctagtagg tgaaaaactc
    20401 tctcacagtg tttcacctca aatccacaag agagtattcg agatattgaa tattgaaagt
    20461 gcttataaaa actttgaaat ttcaaaagaa gatatatcta agctagacgg tgctataaaa
    20521 ttattaggaa tacaaggggt aaatgttact gtaccatata aggagcgtat tatgaaatat
    20581 cttgatttta tatcaccaga ggcaaaacgt ataggagcag ttaatacaat tttacttaga
    20641 gaaaatatgt tatatggtta caatacagat tactttggtc ttgatagtat gtttaaaatg
    20701 gctaatattg atgttcaagg aaaagtagca gttatattag gaactggagg tgcttctaaa
    20761 gctgctctta catattttat tgattctggt atagaaaagc tttatgtttc aactagaaaa
    20821 aaggatgata aaaaattatt aaatagtaaa gctatattaa tagattatga agaacttaaa
    20881 catataaaag gagatataat attaaatgct acaccagtag gtatgtaccc taatgtagga
    20941 atttcaccag tgtcaaaatc tattatacaa aattttgata tattaataga tttaatttac
    21001 aatcctggag aaacagagtt tttaagaatt ggtaatagta tgggtaaaaa aacttgtgat
    21061 ggattatata tgctggtggg gcaagcaata aaatctcagg aaatttggca agatactaaa
    21121 atagataata gtattttaga tgttatatat aatgaactaa aattagaatt tttgtaaggg
    21181 agattgttat gataaataaa acaaaagaaa aattgatatt aataggtatg ccaggtagtg
    21241 gaaaaactac aattggtaaa cttttggcca aagaatataa ttgtagtttc tgtgatatgg
    21301 atgattatat aattcaaata tctcaaaaaa gtatagcaga gttgttttca gaaggagaag
    21361 atatctttag gaactatgag actcaagcat gtagagaact ttctataagt gataaaacag
    21421 tgatatctac aggtggaggt gtaataaaaa aggatgttaa tatggaaata ctaaaagaaa
    21481 caggtataat aatttttatt gatagaccta tacaaaagat tttggaagat ataaatatta
    21541 attcaagacc attattaaaa aacggaaaag atagattgta taatctttat aatgagagaa
    21601 taaatcttta taaaaaattt tcagatatag agattttaaa tgataaatca ttaaataatg
    21661 ctgtatataa tattacaaat gcagtcagtg aaaattttaa atttgacttt aaagaaaaat
    21721 aatctagggg gaaataaaaa taatgaatat tgttattgta ggactaggag ttataggagg
    21781 ctcttttgct aaagcattaa aaaaagctgg atatgaaaat gtatttggag tagatgtaga
    21841 tttagaaact ttaaaaaaag cagaaaaagc aaaaatcata aaaaaaggat gtactacagg
    21901 taaagagtta tttaaaaaag ctgatttaat tatattatct atatatccta gattagttgt
    21961 agattttcta aataataata aaaacttttt taaaaaaggg actataatta ctgatactac
    22021 aggaataaaa gaaacactta tcaatgatgt gttgcaaatt attccagatg atatagattt
    22081 tatatttgga catccaatgg caggtaggga aaaaaagggt attgattttg caagtgaaca
    22141 agtttttaat ggcgcaaatt atataattac tccaactggc agaaacaaca taaaaaattt
    22201 agagttagtt gaaaatttaa ttttagagat tggatttaaa agagtaaaaa agttaacttc
    22261 acaaaaacat gatgagatta tagcttttac ttctcagtta cctcatgtta tggcagttgc
    22321 tcttataaat agtgatgaag aaggtagaga tacgggtaaa tttattggag atagttatag
    22381 agatttaact agaattgcaa atatgaatga agacttatgg agtgaacttt ttttaggaaa
    22441 tagagataat ttactaaaag ttatagaaaa ctttgaatca gaagtaaatt tagtaaaaga
    22501 agctatattt aacaatgata aaaataaatt aatagagtat tttaaaaaat catcaataag
    22561 aagagaagct ttagaaaaat aaatattaat atttaagcta tatatagatg ggtataaata
    22621 taagcgatag tgtatttaat tgtattaata aatatggatt caat
//
LOCUS       DAELTA010000049        22357 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.049, whole genome shotgun sequence.
ACCESSION   DAELTA010000049 DAELTA010000000
VERSION     DAELTA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.049"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(138..1328)
                     /locus_tag="KPG13_001640"
     CDS             complement(138..1328)
                     /locus_tag="KPG13_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0985373.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1588..2019)
                     /gene="dut"
                     /locus_tag="KPG13_001641"
     CDS             complement(1588..2019)
                     /gene="dut"
                     /locus_tag="KPG13_001641"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG0985374.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2132..4360
                     /locus_tag="KPG13_001642"
     CDS             2132..4360
                     /locus_tag="KPG13_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG0985375.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4471..4956)
                     /locus_tag="KPG13_001643"
     CDS             complement(4471..4956)
                     /locus_tag="KPG13_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0985376.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5208..6431
                     /locus_tag="KPG13_001644"
     CDS             5208..6431
                     /locus_tag="KPG13_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0985377.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6435..9569
                     /locus_tag="KPG13_001645"
     CDS             6435..9569
                     /locus_tag="KPG13_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG0985378.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9603..10901
                     /locus_tag="KPG13_001646"
     CDS             9603..10901
                     /locus_tag="KPG13_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG0985379.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11190..11435)
                     /locus_tag="KPG13_001647"
     CDS             complement(11190..11435)
                     /locus_tag="KPG13_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0985380.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11649..14276)
                     /locus_tag="KPG13_001648"
     CDS             complement(11649..14276)
                     /locus_tag="KPG13_001648"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0985381.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14337..15179)
                     /locus_tag="KPG13_001649"
     CDS             complement(14337..15179)
                     /locus_tag="KPG13_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG0985382.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15186..15824)
                     /locus_tag="KPG13_001650"
     CDS             complement(15186..15824)
                     /locus_tag="KPG13_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985383.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(16260..17552)
                     /locus_tag="KPG13_001651"
     CDS             complement(16260..17552)
                     /locus_tag="KPG13_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0985384.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(17589..17945)
                     /locus_tag="KPG13_001652"
     CDS             complement(17589..17945)
                     /locus_tag="KPG13_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0985385.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(17980..18339)
                     /locus_tag="KPG13_001653"
     CDS             complement(17980..18339)
                     /locus_tag="KPG13_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985386.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(18408..20381)
                     /locus_tag="KPG13_001654"
     CDS             complement(18408..20381)
                     /locus_tag="KPG13_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0985387.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(20603..21616)
                     /locus_tag="KPG13_001655"
     CDS             complement(20603..21616)
                     /locus_tag="KPG13_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0985388.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(21629..22174)
                     /locus_tag="KPG13_001656"
     CDS             complement(21629..22174)
                     /locus_tag="KPG13_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0985389.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         7667 a         3731 c         2890 g         8069 t
ORIGIN      
        1 agagaaaata aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt
       61 actatcaata ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg
      121 ttttataata aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag
      181 ctactattta tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata
      241 tctccatttc ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta
      301 tcaaagaata ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta
      361 ctttggtctc tagatactct tggtatacta actcctacag attttttcat aacgtactgt
      421 gtgaatccag aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca
      481 agtagtgtct ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa
      541 taagatggaa ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat
      601 ccagtttgat tgccataact tacttttgtc catcctccac tttcagaaat acttccaacc
      661 tcagtaccat aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg
      721 ttcaatgact ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca
      781 gcattttcat ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct
      841 actctaccat tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca
      901 gtcccatatc ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt
      961 aatccttttg ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta
     1021 cttccaaggc tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat
     1081 ttaactttta cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt
     1141 ttccccattg attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt
     1201 attgttctgc tttctatact cacactctct acttcagact cattagcata tgctaaattc
     1261 cccatagtag gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta
     1321 aaattcataa ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat
     1381 aaaagaaata attgacacta ttaaaatatc aattatttct tttatattaa tattattaca
     1441 aatttgtaac tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt
     1501 taatctttaa tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc
     1561 tatatttttt aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc
     1621 tctttcggag tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac
     1681 tatcatttga gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat
     1741 taatataatt ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac
     1801 agtaacagaa tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt
     1861 aggaagctct atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac
     1921 ctcttctata gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat
     1981 agcatcatca tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt
     2041 ctataaatat tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata
     2101 taattattag tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc
     2161 tgtggcaaaa actatagcat catttttagg tgcaaagacc agacaagatg gttactttga
     2221 aggaaatgat tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa
     2281 ggattatgat aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc
     2341 tgaggataag tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa
     2401 aaagcttttg aatagaaaag atgtagaata tgtaataaat gccactgaca atgaccgaga
     2461 aggagaactt atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag
     2521 aatcttggta aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga
     2581 tgaagatgaa atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt
     2641 gattggaata aatttcactt ctgttgctac attgaaatat ggaaatggga aactattaaa
     2701 cataggtagg gtaattcttc caacagttaa attagtctat gatagagata tggaaatact
     2761 aaactttgtt cctaaaacct attatgaaat agaagggcat tttaaagctg aagctggtga
     2821 atataaaggt aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa
     2881 taaaattata gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat
     2941 gtcaaaggaa tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc
     3001 taagtattcc aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg
     3061 taatggtaag ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag
     3121 tttggcttca aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa
     3181 taaaattaaa ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag
     3241 tgctataatt ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta
     3301 tgatgccata aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac
     3361 agaaattaag actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc
     3421 aaaaggatac cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa
     3481 taaagatgat gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc
     3541 taaaccatat actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc
     3601 tgaagaagat acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga
     3661 tgtgttaaag aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat
     3721 aacagacctt gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga
     3781 ttatactgga aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa
     3841 agaatatctt actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga
     3901 tgcttcaaaa aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac
     3961 taaaacaaaa gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat
     4021 atcaaaggat tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg
     4081 caaatgcccc atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa
     4141 tcataaagaa tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa
     4201 gaaagttaac aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc
     4261 tttaactgct aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga
     4321 tactggctat ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat
     4381 agcagtagta taataatcta tattatatta ctgctatttc ttttcttatg aagttttaac
     4441 ttttttaata gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa
     4501 ctttaatgta tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt
     4561 tactagagat tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt
     4621 taattcaacc ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg
     4681 ttccactaat ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc
     4741 tatttgagaa attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga
     4801 tggagccatt ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt
     4861 ttttaacaaa tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa
     4921 agagtccgct attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat
     4981 aatataataa aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa
     5041 acaagaaaaa gttattgact cataataatt ataaatttat aatcaaatta gagaattata
     5101 aattgccaat aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac
     5161 tttaaattct acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt
     5221 taatagtttt cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat
     5281 ctgaacctga gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa
     5341 acacaaattc tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga
     5401 ctgttggcac agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg
     5461 aattattgag tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac
     5521 tagatttagc aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa
     5581 atcaggcaaa aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt
     5641 atgacaataa taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa
     5701 tagaattttc tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata
     5761 cagcaacttc aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag
     5821 ctcaagtatc atataattta gcaatgagta atttagacaa attaacatta acatctccag
     5881 tagatggtac aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac
     5941 cagcttttat tatttctaat cctagtatac tagaagttga tttaaatgta gcagaatcag
     6001 atataggtaa atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt
     6061 taggaaaaat agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta
     6121 aagtacttgt aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact
     6181 taagtgttga aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa
     6241 atggtaaaaa atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc
     6301 aaactggaat tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata
     6361 cagtgattat tggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa
     6421 aggagaagta gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa
     6481 tggtcttttt agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc
     6541 taatgcctga tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc
     6601 ctcaagacgt tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa
     6661 atgtaaaatc tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg
     6721 aatttggaac taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac
     6781 agaccagcct cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac
     6841 aggccatagg tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa
     6901 aatacgctga agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag
     6961 accttaaagg tggaaaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa
     7021 attatggggt tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc
     7081 cttatggttc aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag
     7141 aatccctaga tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat
     7201 tagataatct ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca
     7261 atggaaaaga cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag
     7321 ttatgcagag tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa
     7381 ttaaaattgc taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca
     7441 acttgcttat aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa
     7501 gagcttcact agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc
     7561 tctactttac tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg
     7621 gtatggttgt tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg
     7681 gcagattgag tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac
     7741 ttgcatcaac attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag
     7801 ctaaaataac ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta
     7861 taggtgcaat tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc
     7921 aaaagatgca agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat
     7981 tattaagtgt atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg
     8041 tagcttcctt atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg
     8101 gtgaattatc tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact
     8161 atgtatctat gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa
     8221 ctttgacatc agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa
     8281 aaaatagaaa aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg
     8341 taccagattg taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag
     8401 cagacttcac attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag
     8461 aagctgaaaa agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag
     8521 atacaacaca agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa
     8581 ataccgctga aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg
     8641 ctactattga tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata
     8701 gcttagaaga tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg
     8761 gtgctttttc agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag
     8821 atttttctgt aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac
     8881 tagcactaga aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg
     8941 gtgaaacaga aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta
     9001 tagcattagt ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa
     9061 tcatgttctg tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc
     9121 aaagtttaag tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata
     9181 atggtatagt tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt
     9241 taattggttt ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag
     9301 caacaacaat tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta
     9361 tgcaaccact agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg
     9421 tgcttatacc tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa
     9481 tgcctaaaat atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa
     9541 agaaaccaaa attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa
     9601 aaatgagcaa aatagttgca attggctgtg gtgttggtat aataactgcc aatttaatcc
     9661 cagtttacgc agataataat acttcttcta taaacaaagt tgataattct attttaagtg
     9721 actcaaataa aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg
     9781 cacttaaaaa tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa
     9841 agttagaagt acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg
     9901 accaaattgg acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa
     9961 tagcaaatga tattactaat aaatttaacg atttagtttc aagagaaaat gagctagaca
    10021 agataaaaaa caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag
    10081 atttagggtt agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac
    10141 aaaatactca aaaagctaaa cttcaagaac taaaaaacaa tcaagactac tttaagttgt
    10201 tgactaatat agatttaaat aattatcaat tggataaaga atatagattt gaatctttta
    10261 gagtaggtgg ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg
    10321 accaattgat tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag
    10381 atttaccaga tagacctaat cttacaagac cagttgctcc tacaaaacca gagcaaggta
    10441 acctaactga tgatgaatat aaggttctta cggataagta tgaaaaagac tacaagagat
    10501 atgaaaaaga agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta
    10561 cttatgcaaa ttatcttcaa caaaaattta ataccgaaaa tggtctagta acattagaag
    10621 acagtaaaaa agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag
    10681 aagatacaat tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata
    10741 ctaaattaag atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa
    10801 gtaatgaaga tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa
    10861 atggtataga aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat
    10921 tactttaaca tacgtttgat taaaaaatag ttatgcacac tcgtggcttt agtcatggaa
    10981 gattcagaag gcaaataata gaaatagatt agtaaaaata tatttgctta taaaactgta
    11041 taattttata gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata
    11101 aaaatatatt taattccata caaaaagagt gccttatgaa ctaaaaagtt cattttgaga
    11161 caccctcaaa agcttaactc taaatttaat tattttttat caaatgtctc atctatttta
    11221 catctcacca tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta
    11281 ggacaataat ccaatttcct agataccaaa gccctacaac taggttctgg acatcttatt
    11341 tttagactct catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat
    11401 gatacttcct ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat
    11461 ttaattgata gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc
    11521 acttacaact atataagtta aatttttata ataaaaatcc cctatattca atttacaaat
    11581 acttaatttg atacattgaa caatagggga ttttattttt atttagttac atattttgca
    11641 gtctattatt atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc
    11701 actctaaatg gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa
    11761 gatggttctc ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta
    11821 cctaatttag ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat
    11881 gggtcttttt ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct
    11941 cttgaatatc caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct
    12001 tcttgtgcta tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca
    12061 gtataaggaa cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt
    12121 tttcttatta tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa
    12181 actttaacac ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt
    12241 acacatattt caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta
    12301 aactcatcta aatcaactat tctcttctta atatcactta cttttattcc catagatttt
    12361 gaaagttctt ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt
    12421 aatcttatat taactggttt tccatccata actttaaata actctacaaa atcttctctt
    12481 tgcattggta atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc
    12541 atttctctaa ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga
    12601 cctatacctt ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta
    12661 gttctaacca tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca
    12721 gttaaagata cttctgtttt cttaacatct cctaggtata catttcctgt agaaccatct
    12781 atagaaatgt attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta
    12841 aactcatcaa cttttatttc tccacatcca gcaacacaac atttacccat acctctagca
    12901 acaactgctg catgtgatgt cattccacct ctagctgtaa ggattccttc tgctttaacc
    12961 attccttcta tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt
    13021 tcatttgctt ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt
    13081 gatgctggta aaccttttgc tataacctta gcttctttta atgccttatc ttcaaatttt
    13141 ggatgtaata attggtctaa ttgattaggc tcaattctca tgatagcttc tttttcatca
    13201 attattccag cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta
    13261 ccatttctag tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc
    13321 atatctttat aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct
    13381 ggcattacat ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct
    13441 tcaccttgtg cattcattaa aaattcacca aataatttat tttctcctgt agctggattt
    13501 ctagtaaatg caacacctgt accactagtt tctcccatat taccaaatac cattgattga
    13561 atatttactg ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca
    13621 cgaggattat tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg
    13681 tcttgtggga aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt
    13741 tcttttaaat catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact
    13801 ctgtctaata cattttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt
    13861 tgtatgaatc ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag
    13921 ccaacaacac tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata
    13981 gagaatactg caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt
    14041 ttatttaagt ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct
    14101 cttatacttt cattgttaac ataataatca ttacatgctt ctgttgttat agtgaatcca
    14161 ggagggacag gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt
    14221 aaagatttca tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt
    14281 aaatccccca ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa
    14341 atttctattt ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct
    14401 gctgtttctt ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt
    14461 atctcttctg aataatctaa ctcctgaagt attctttcta aattagcata gtttgcttta
    14521 ctggacaatc ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa
    14581 ttagtaagac ctattatctt tttagtatct atctcaaaca cttctttagg aattggtatt
    14641 tctggaacta atggaacatt agctactttt atatttttat tagctaaata catactcaaa
    14701 ggagttttag atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta
    14761 gggtcttttc catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa
    14821 gattcatcta attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg
    14881 cttatttctc ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa
    14941 tatctttcaa caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt
    15001 tcagcttttg cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat
    15061 ctttttattt cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt
    15121 tgtgcagtct cccctactga atctgatact gcatatataa ttaggttttt caccagcata
    15181 cctccttaat tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc
    15241 ccaacaactc tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct
    15301 acttcatgtt ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg
    15361 actacatttg gagttcttgt cattatcata cctattggca ttttatttat atcagaacct
    15421 cctatagttg cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca
    15481 tctataataa ctatactccc tacatcagat aaaaacatag tgactattac atcatatata
    15541 ctttcatcct tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt
    15601 acacttttac caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt
    15661 cttgcgtcta atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga
    15721 gtcacattca agctggaggc aatactttca ctcgttattg gttcattttc cttaactata
    15781 tctataattt ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct
    15841 atacttttta tcttttcgca tcttatatag tatattatat aactatatat accattctgt
    15901 aaacagattt tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt
    15961 tattatatat taatggggga tatttcttta attaatagaa ctatattagt attacagtta
    16021 atattataat acataattgg tcattttaat tcattttata ttaaaatttt taataatatt
    16081 atttcaatgt gaatataaaa ttgcagaact aattcaaaat aatttttacg actacaaata
    16141 acctataata tacttttaaa gaaagtatcc taacaagaat atataaaaat ttaaaagggt
    16201 tgatttaaaa ataaccatca acccttttta ctaatatcta tttatattac catcaaatct
    16261 tacccaaata ttttttcttg caattgtctt aaatgatata aagtagtatc tttttctatc
    16321 tctaccatat cgtaagttat aacttcacct tttttaatat cttttacaac tttagtcttt
    16381 ttacttatca aaccaatagg tacagcattc atagcctttg ctacatcgta tttttctata
    16441 gtaccatagc atgtatatgc tccatagcca tccataaact cacctttttt caaatctttc
    16501 ttagctatag tcataacttc tgctactagt ccagctttag gtactattgt agccttatgg
    16561 tctaaaactg ccatagctgc tgataaagga gtctctaaac tacataaatg atatggtcta
    16621 tataatatgt aattaggtcc atcacccata cttaaatatt ttagttcatc attaactgct
    16681 tttaatttat gggcaactac tacgaaaact ccaggtgcta taccatttac aaaatcaact
    16741 acaccataat tatctaatac tccaccttcg ctttttaatg catatttctt aggtacttca
    16801 ttaactgtag cttcaatacc atgaccacct cttacatcag gcacaaatcc agttgcatta
    16861 cacattaaag ccatttctac cattgtttta gttccttctt taaaagaagc tatcatatga
    16921 ggtgaagctc cttttctttc tgcttcttct ctaactgtat caggattaca atctaaatct
    16981 aatttattat ttttaccttt acctatcact ctaacttcaa accccattgc atctgcaaaa
    17041 tcaaataatt ccattactgc acctggctca tctcctgcag aacctgtaaa tattactcca
    17101 gcatcatctg ctaatttttt aagtattggt ccaacaacac aatcagtttc agcatttaac
    17161 ataactatat gttttttatt taaaatagag tttaatgcta tctctgcacc aattgcaata
    17221 cccccagttg catctataac tacatctatt gacttagttt tacaagccac aagaaagtca
    17281 ttacatgtca atacttttcc ttcttgtaat tttatttctg catccttttc atttgttaca
    17341 ttatctataa tttcttcatc tattcctgca tcagtaaaag ctttatgagc caactccaaa
    17401 tctatatcta caacaataga agcttcaata cctttcatat ttgccatttg gcttaccatt
    17461 ccatttccca tatgcccagc tcctataaga gctactttta tttttttacc ttcctcttcc
    17521 aattttgcaa gttttctgtt taaatctaac ataccaattc ctcctgttat aataatttta
    17581 ttttcaaatt aagaaatagt tattaaatct ccaatagtta tatctggatt tcctatttcc
    17641 acatgtattt ctcctggtaa atttacttca cattttccat caaatttgat tgtacaatgc
    17701 ccaatttctc taagagtctt ctgagcaata tcaccaactg aaactataca ataatctcta
    17761 ttccctatag taagtgtatc tcctatttca acatcacttt ttaatgttgc tattgaatga
    17821 attacagata tatcctttaa gtcgtcatca ttaatggttt catcatatat aataagacaa
    17881 ttgctttcat ccattaactc tttaactaaa ctgcctatcc cacttatctt aacttcatat
    17941 ttcatgacat tcctcctgat aatttatatt tatattgagt taattaaaag aaacatttat
    18001 tttttctttt ataaatttta tagcttgtac aatactctct tcatctttaa atttttgctc
    18061 tgcactatat atatctaatc cttctatttc ttcaatctct ttaaatttag ccattactgt
    18121 acgccctttt aaaatttctc caaactttat aaacccatct gaatctgaag atataacggc
    18181 tatacttcct ttagaaaaat agcttttctt tttgcctgta gacactatat cacccttttt
    18241 tttagctcta tttaaagttt taaaatacaa tttattctgc ttcattataa atatagtatt
    18301 taaaatataa gcaacaaata aaattactaa taatttcata tttatacacc ttccaaaaat
    18361 cacattattt ttaaatctgt ataacaacta taaatgctgt acaatagtta tataaggttt
    18421 ttaagtaaca cctttttaaa ttctaaaaaa gttctacatt tacttatctt attcgttaat
    18481 tttacatctt ggcctatctt taaaacttct tcataaattt ttactaataa gtctgagtct
    18541 atcttattct cacatggaaa cattgtcata agaattattt ttatacttct atttgcatat
    18601 tctattggtt cttctaaaat tccaactgcc attaaaaact tatctgattt ttcatttata
    18661 gaatgaggaa ataataaacc tttatcaaat actgtgggag atttttcttc tctattgact
    18721 atattttttc taaacttctc atcaatacat cccaaatcca taagatggtc catcattttt
    18781 tctaatgagg caagtatagt cttttcattt aatatcataa atttatcttc atctaacaaa
    18841 ttatttatta gtaaatttgt agaattagta gtgtcaatat tcccttctct taggcacaat
    18901 actgttttta attgttctct tagtttgttt tcatcaaata ttgtatttat ttttaatact
    18961 attgtattta tatttgatgt gtctaaatca atagttgtaa aaactatatc gtattcatct
    19021 aagttaatat ttctcaaatt aaaacttgaa agagtatcaa ttttaggatt atttcctatc
    19081 agtttttcta ctcttatttt caatagtttt gctgttccta gacctgtact acatataatt
    19141 gctatttttt ttattgaata gaatctactg ctatttcttt ctaaatatcc actgaaatgg
    19201 attgctatat atccaatctc atcttctgaa acttctaagt tatacttttt ctctattact
    19261 tttcctgcta tttgtgctaa tttaaatgga agtatataat tttgcttaat ctcttctaac
    19321 aaaacatttt ttaactttat attaaataat agcctgttta aagtaaaact aagatgatat
    19381 cctagaccta ctcttaattc atcctcattt ataaaaatac ccatatcgac atatatttga
    19441 ttaaatatat tttccatcaa ctcatcaatg tttttactaa ttctactact atttaaagca
    19501 cattctgatg ctggtgcatt tccactaact aacggtatag ttaaataaat aatttcttca
    19561 tcatttaaag tacattcaaa cattcctgat attatatgtt ttatttccct agcaataaag
    19621 tattcatcat atgatttaag taaatcaaac tttacatcat aatcttttac tttcttttct
    19681 tcttttactc tatagactaa aactactatg tagttaataa cttctcttaa catttcatct
    19741 gtaacataaa aattattttt tttaaataaa gtcaatagtt tttcttctaa atttatgatt
    19801 tcttcttttt tgattccttt aaaatacttt gaattactga aatctttcct tgagttttta
    19861 tacaactgat ttaatataaa cagtctaata tcaagttcat ttccatttaa tttcatacct
    19921 gtattctgtt ttttaatcaa gtttaaatta tatgaagcca aaactttttc tacatactgg
    19981 aaatcattta ctaaagtagt tcttcctaca cacatctcat cagctaaact atcaagtgta
    20041 ataaatccat ctagttctat caagtaattt agtataaact ctactctatc ttcttttgaa
    20101 tttaatgaaa atacattttt tctattcata tcaataatct tattgagtat ctgctcatct
    20161 tttatttcta gtttatagcc ttgacccttt atatatttga ttactgctat atcattacct
    20221 aaatctttat tcaattgttt tatatacgtc cttacacttc tagttgtgac attcaattca
    20281 tttgctattg agttaatact cacaaactct tttttactac acataatcaa tatcttaaat
    20341 atcttgcggt caatagaagg aattttctca gttacactca ttttgtatct cctccttgat
    20401 ttcattctag tacaaatttg aaaccaaatt aatacataaa atttcacttt aataaaggaa
    20461 aatttagtat taatctaact cgataattac agctaattaa tagtataaaa gctagtcttt
    20521 cattaacaaa aaatcttgac tagcttaaat ttatatttac aattttatta attaattcca
    20581 agccttatat tgacattaaa tattatctca atgcaaatac tgcaactcca tatgcaataa
    20641 taacagctat tggaccagtt attaaacgag agtaaaataa agctggaaca ccatactcta
    20701 cagtttctgg ttctgcttct cccaaactta atcctactgg tacaaaatca catccagctt
    20761 gcccatttat tgcaaataaa gctggaagtg ctaaatatgc tggtattgca ccaagaccaa
    20821 tttgtgtacc tacaagagtc cctactactt gagctataac tgcaccaggt cctagtattg
    20881 gtgataagaa tggaagtgtg caaattactg atataatcaa taatccacca atattacctg
    20941 caagtggaga tataactctt gcaatccagt ctcccagtcc agatgctaaa ataattccca
    21001 taagcatact tacaaatgcc ataaatggta ttacgttatt cattaccatt tggatagtat
    21061 ctcgacctgc atcataaaat tttgatacta cttgccctgc cccttgacca atagaaagta
    21121 ttatgttctt tttagcatat tcaccttgtg cttttgactt aacctcatca taattatcag
    21181 gctttctaat agaactctta ttttcgcttt tattttcttg agatttttgt ggcatatctt
    21241 ccccatctac aacacttata cagttaggat taacatctga tacataatat tcttctgtta
    21301 taaattttgc taaaggacct gtttttccaa ctggatttac atttattgta ggtatttttt
    21361 tctttggata aactccacat ctagcagttc caccacaatc aataataact actgctactt
    21421 cttcatcaga aactccagtt ttaaatccat ctacaatttc acaacccagt agttctttta
    21481 tttttaatgt tacttcatgg ataccgccag cagtcattga tagtatctta tttctattcc
    21541 cttctttttt tatatacaaa ggacctcccc agcctgaagt tcccttttct atttttaata
    21601 ttttattctc ctccatttta tctacctcct aagccttatt tgttgctcta ttttttcttg
    21661 ttagaaaagc atacattttt tctgttacta tacctcttat aaaaattaca attagaccaa
    21721 ctataaaata ccacaatgcc aatgtagtag tttctttacc taaagtagtc aaacctgcag
    21781 atattcccaa ccatacaaaa agttctcctg catttgcatg tggaaaaaga cctgtaattg
    21841 gatgtacaaa tgaaactgct gcatcataaa atcctgcttt gtgctcttct tttaaaaatc
    21901 taccaaatgt atagcacata ggatttccca agaaaaatac tgatactaat ggaagtagaa
    21961 tatatcttgt aaaagagttc tttcctagct tagaagccca attattaact ctttcctcac
    22021 caattatctt aatcaaagag ttaatagcta ccattaatac aattagcgtt ggaagagtac
    22081 ctgaaacaag acctataaat tgttctcccc ctttctgaaa caaaccgata aaccactctg
    22141 cacctttact tagacctgta ataacgtttt ccataaaatt ataacctccc tataatatct
    22201 ttaaattttt aattttactg cttaccataa attttacaaa ttacatcttt tttctgtact
    22261 atatgctaat attgtaaatg tttttttatt aatatcattt taatataata tttttgataa
    22321 ataaatacat cttttttcaa ttttataaag gaaaaaa
//
LOCUS       DAELTA010000050        22203 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.050, whole genome shotgun sequence.
ACCESSION   DAELTA010000050 DAELTA010000000
VERSION     DAELTA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.050"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            70..660
                     /locus_tag="KPG13_001657"
     CDS             70..660
                     /locus_tag="KPG13_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985390.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            774..1619
                     /locus_tag="KPG13_001658"
     CDS             774..1619
                     /locus_tag="KPG13_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985391.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            1616..2299
                     /locus_tag="KPG13_001659"
     CDS             1616..2299
                     /locus_tag="KPG13_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985392.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            2296..3012
                     /locus_tag="KPG13_001660"
     CDS             2296..3012
                     /locus_tag="KPG13_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985393.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            3109..3372
                     /locus_tag="KPG13_001661"
     CDS             3109..3372
                     /locus_tag="KPG13_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985394.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            3344..3799
                     /gene="def"
                     /locus_tag="KPG13_001662"
     CDS             3344..3799
                     /gene="def"
                     /locus_tag="KPG13_001662"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG0985395.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            3851..4312
                     /locus_tag="KPG13_001663"
     CDS             3851..4312
                     /locus_tag="KPG13_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG0985396.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            4362..5264
                     /locus_tag="KPG13_001664"
     CDS             4362..5264
                     /locus_tag="KPG13_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985397.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            5634..6446
                     /locus_tag="KPG13_001665"
     CDS             5634..6446
                     /locus_tag="KPG13_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0985398.1"
                     /translation="MYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            7495..8256
                     /locus_tag="KPG13_001666"
     CDS             7495..8256
                     /locus_tag="KPG13_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0985399.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            8321..9103
                     /locus_tag="KPG13_001667"
     CDS             8321..9103
                     /locus_tag="KPG13_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0985400.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            9370..9789
                     /locus_tag="KPG13_001668"
     CDS             9370..9789
                     /locus_tag="KPG13_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG0985401.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(9892..10158)
                     /locus_tag="KPG13_001669"
     CDS             complement(9892..10158)
                     /locus_tag="KPG13_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG0985402.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            10385..11086
                     /locus_tag="KPG13_001670"
     CDS             10385..11086
                     /locus_tag="KPG13_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985403.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            11168..11776
                     /locus_tag="KPG13_001671"
     CDS             11168..11776
                     /locus_tag="KPG13_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0985404.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            12100..12564
                     /locus_tag="KPG13_001672"
     CDS             12100..12564
                     /locus_tag="KPG13_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBG0985405.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            13201..14217
                     /gene="gap"
                     /locus_tag="KPG13_001673"
     CDS             13201..14217
                     /gene="gap"
                     /locus_tag="KPG13_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0985406.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            14612..15781
                     /locus_tag="KPG13_001674"
     CDS             14612..15781
                     /locus_tag="KPG13_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985407.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            16161..20414
                     /locus_tag="KPG13_001675"
     CDS             16161..20414
                     /locus_tag="KPG13_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0985408.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRRRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            20563..21057
                     /locus_tag="KPG13_001676"
     CDS             20563..21057
                     /locus_tag="KPG13_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0985409.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            21173..21550
                     /locus_tag="KPG13_001677"
     CDS             21173..21550
                     /locus_tag="KPG13_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG0985410.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            21756..22196
                     /locus_tag="KPG13_001678"
     CDS             21756..22196
                     /locus_tag="KPG13_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG0985411.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT         8966 a         2147 c         3788 g         7302 t
ORIGIN      
        1 tattgacatt agatagaaaa agtattatat tgactatata aattatttta gtcaaactgg
       61 agggaaaaga tgcctaaatc gtattctgat aaagaaagag agtatataat aaaaagactt
      121 aaagaagaag caagattgtg tatggagcaa tatggtatta gaaaaactac tgttgatgag
      181 cttgtaaaac gggtaaaaat accaaaagga actttttatc tattttttca atcaaaagaa
      241 ttgctctttt ttgaggtatt aagagatata catgactcta tacaaaaaga aatcttatat
      301 gagataaata gagtagatga aagtattaca tgtgaacaat tgacagatat atttatgaaa
      361 ttctatagga tggttgatag tacttcaatt ttaaatctta tgataaatgg agaatttgag
      421 atattggtga gaaagttacc agattctata attgaagaac actttagaca tgatgacttt
      481 gaaattggag agattatatc ttgcatacca aatgcaaaaa ataaagacat agaaagcttt
      541 agtggagcat ttagagcagt ttttcttacc atgctatata agcgcgaggt tggaagtaat
      601 tgttttgaag atgctttaag attaatgata aacggattag ttatacaact tatggaatag
      661 aatttaaatt ttgtttgcta ataactttta ttaaaaatta ttaacaaaca aaaaaattat
      721 attttattga ccatatagtc aaaatagtca aaaatttgaa agggtgatat tgaatgatta
      781 aagttgatga tttatcattt agttatacag agagagattt tttacaaaat attaactttg
      841 gagtaggaaa aggggaaata ctaggatttt taggaccatc aggtgcagga aaatctacat
      901 tacaaaagat tctaattgga atgataacta attatggagg aagtgtcatt gtaaatggag
      961 tagagagtaa aagacactca aataaatttt atgaaaatat aggagttgat tttgaatttc
     1021 ctagtttata tgaaaagttg actgcaatag aaaatttaaa atattttggg tctctatatt
     1081 ctaaaaaact actatctatt gatgagttat taaaatcagt aggactagaa aatgaagcaa
     1141 ataaaagggt atctgagtat tcaaaaggta tgaaatctcg attaaatttt ataaaagctt
     1201 tattacataa tccagatatt ttatttttag atgaaccaac aagtggtctt gacccatcaa
     1261 atagcaaggt tatgaaagat ataattttat cagaaaaatc taaaggtaaa actattattt
     1321 taacaactca taacatgtta gatgcaacag agctttgtga cagagtagcg ttcattgtaa
     1381 atggtaaaat atctgcttta gatacaccac ataatcttat tatgtcaaaa ggagctatta
     1441 aagttagata tacttacttt gataatggag aaaaaacttc tgaatgtttt ctgaacaata
     1501 ctgctaatga taaaaatttg aatatgctta ttgagaaaaa taaactttta tcaatacata
     1561 gtagtgaacc aacattaaat gatattttta ttgagataac agggaggaat ttacaatgag
     1621 gctaaaaaat cttattttag gagatataaa gtttcaattt aagtatggtt tttatttttt
     1681 atatcttttt ttaagtatta tatatatttg tattatcaat gtatttccaa cttttatgag
     1741 agaaaaaata gctataatta tgatttattc tgacccagca gcaatgggat tgttttttat
     1801 gggagctatt gtacttcttg agaaaagtca aagggtttta aattccttag ctgtatctcc
     1861 agtaaaagta tctgagtata ttttatcaaa ggtaatttca ttaggagtaa tatctagtat
     1921 tgtagctatg tttattgcta ttacattaaa tttagataat attataatat caactatagg
     1981 tacctttttt tcttctatta tattttcttt gcttggattg atacttgcaa gtaaagcatc
     2041 tagtttgaat cagtttattg ttttaagtat tcctattgag attatatgct ttattccacc
     2101 aatactcaat gtacttcttg acacaaaatc atatgcaaat ttgtaccctt ttaatatatg
     2161 tatttctctt attagtggag ataaaaattt tataatgatt aatattttaa ttttaatatc
     2221 aattataata ataatttatt ttataactta ttattttatt tgtagcagtt ggaaaaaggt
     2281 aggtggagta aaactatgat aaagattata aatgcgttta aacaagagtt tgaacaaatt
     2341 aagagagatg ctatgctttt tatagtatgt gtttctccaa tattatgtgg agtatttata
     2401 aaatttggaa taccattaat acagaatata tcacttaaca agttttatta tcaattaaat
     2461 ttagagccgt atttcttgat gtttgattta cttttagctt ttattacacc ttttatgttc
     2521 ttttttgctt ctacaatggt catattggga gaaattgatg acagcatttc aagatattta
     2581 atagtaactc cgcttggtaa aactggttat cttatttcac gatttggaat accaggtatc
     2641 ttagcattta ttattactat gattctactt atcttttttt ctctaactaa aatttctttc
     2701 ttgttaaact tatcaatttc tttattagtt ttattacaag gaattattat atcaatatta
     2761 gtgatttctt tatcatcaaa taaactagaa ggaatggtaa ttacgaaatt ctcaggtgtt
     2821 tttatgatgg gtattttagc accatttttt attttaaata aagcacaata tattctgttc
     2881 ttcttaccaa ctttttggat atcgaaggca tttaaggaag ataattatgt ctatatgttt
     2941 atttcaattg ttatatcatt aatatggatt ttattattat ttaaaaagtt tagtaaaaaa
     3001 atagttaaat aagaaaattt aatatataaa tgtaaggttg acttgacagt aatgttgaaa
     3061 aatgttacat ttaagatgta aagttaacct tacatgaatg gagcaaatat gaaaaataaa
     3121 ataaagatat taagagaaaa attaggattg acacaagaac aactagggag attagttgga
     3181 acatctagac aggctataaa tgctatagaa actggtaaaa atgaaccttc aatatggttg
     3241 gcatatgata tttcaagaat ttttaatgaa ccaattgaaa gtgtattttt atttgaagaa
     3301 agtgaaagaa aatcaagagc acagataagt aggggagaat attatggcta taagaaagat
     3361 aagaacattt gatgatgaaa ttttaagaaa aaagagtaag tatgtagaaa acgtagataa
     3421 taagataaga gaaatattaa atgacatggc agagaccatg tacaatactc caaatggagg
     3481 aggattagct gcatgccaag taggagtatt aaagcgatta gttgttatag atttaggaga
     3541 aggacttatt aagcttgtta atcctgaaat tattaaacaa gaaggggaac aaattgttgt
     3601 tgaaggatgt ttaagttttc ctgaagtatg gggtaaatta aaaagaccaa aaaaagttac
     3661 agtgcaagct ctaaacgaat acggtgagaa aatagaaatt aaaggttcag gttttatggc
     3721 aaaatgtctt tgtcatgaga tagaccattt aaatggtata gtttttacag ataaaattat
     3781 tgaacatgta aaattgtaag atagattggt tattgaattt ataaaatatt ttatagtaag
     3841 gagttaaatt atgagattat ggcataagga tttaattgat gtattaccta aaaatcaatt
     3901 agtaagtcaa tggagagagc tacttgctat aaaaggttct atagataaaa aaggtactcc
     3961 aaaccatcta ttagtgaata aagtattgaa ttatagtata gatgaattta aattttatac
     4021 aaaaattgta catgatgaaa tgttaaaaag aaattataaa cctaatgaac ttaaatatac
     4081 aagtatatta aaatggaaaa atagaaattt tgcaaatgat atatctaatg aacatagttt
     4141 aaatttagaa aatttatatg atgattggca taataaaatg tatttaaagc aatgcttata
     4201 taatttagaa gaaaaagcaa catgtggagg aataccaata aatgaatgga atatattact
     4261 ttgtaaatat agtaaagatt atgaattgtg gtctggaaat atcatgtttt aattactata
     4321 gattaacatt atagtattat tgtatataaa gaggtgatta tttgtataat aaaataaaat
     4381 atattccaat aaattgtgaa atagcttgta ataaattaaa aaggagtatg ccatataaat
     4441 gggatttaaa tatttatcgt ggatgtgagc atggatgtaa atattgttat gctatttact
     4501 ctcataagta catcaactcc aataattatt ttgaagagat ttatgtaaaa acaaatatag
     4561 tagaaaaact tgagagacaa cttaaaagca gtaaatggaa gaaagaagta atcaatatag
     4621 gaggagttac agatagttat caaccaatag aagctgacta taaaattatg ccagaaatat
     4681 taaacctgct aataaaatac aagacaccag ctattatttc tacaaagtca gatttaattt
     4741 taagagatta cgatttaatt gataagctat ctaggattac atatatcaat gtagcatcta
     4801 caattaccac agtaaatgaa gatacacaaa ggcttattga gccaaatggt gtggattcaa
     4861 tgagacgatt taaaatgtta aaagaattta gaaaaactaa cgcatcagtt ggtctacata
     4921 ttatgccaat tataccatat attacagatg attttgataa tataaattcg ctatttagac
     4981 atgcaaaaga atgtaatgta cattatgttt tacctgggac tttatactta aggggtataa
     5041 ctagaggtgt tttttttgac tttgttaaaa aagagtttcc tgaattattt gataaattat
     5101 caatactata tagtgaaggt tcacttaata gggagtataa aaatcaattg tataaaatgg
     5161 ttaatgaact tagatataaa tattctctat caagcagtta ctctaaaata atgaaggaaa
     5221 agttaaaaaa ctcaggagat gtacagatat ctttttttga ttagttttat ataaaaaaat
     5281 tttttttact atgatgatta aaatttatta aaattaaaat ttattgacaa atataaaatt
     5341 taaatatata attaattcat aaaataaaaa tatgaaagga tgatgttgtt cggtgatatt
     5401 tgatatcata aaatcgttga tataagtatt gctggaaaag agtgaataaa atcaaattta
     5461 ataagcaaac aaagtaaagc tatagtttta ttgttgtgct taaaaatatt tggttctatt
     5521 ttactcttat ttaaactcat ctttttgaag ttaaaccaca gagtaaactc tgtggttttt
     5581 ttattttaaa aataaatttt aaggagtggt atgttttgtt aaacaaatta tgtatgtatt
     5641 tagaaagacc agagctttat aaacaaagtg aaattaattt ttgggatgat gagtatattt
     5701 cagagcaatt attaaaagca cacttagaca ctaattttga aggagcaagt agaaatttta
     5761 actttatcga ggattctgta aattggatag ttacagttgc aaatccagtt aattatccta
     5821 aattacttga tttaggttgt ggtccaggtt tatatgcaga gaaatttgca caaaaaggtt
     5881 ataaggtaac tggaattgat ttctcaaaac gttctattaa ctatgcacaa aatagaaata
     5941 aggagactaa tttaaatata aattacttgt ttcaaagtta tttaaatatg aactataatg
     6001 aggaatttga tttggctacc ttaatttatt gtgattatgg agctttgtca actgaaaata
     6061 gaaggttatt aatggaaaaa atttatgata gtttaaaacc tggaggaaaa ttaatattag
     6121 atgtatttac aataaataaa tacaataatt ttgaggaaat taaatattgg gaaataaatg
     6181 aagatggagg attttggagt aatgaaaaat atatgtgtct acaagataat tgtaaataca
     6241 atgattataa tacattagaa caaactcttg taataaccga aaaagatgaa aatgtatttt
     6301 atgtttggaa tcactatttt tcaaaagaaa gtttcttatt agaagttgaa aatattggat
     6361 ttaagtctgt tgagtttttt agtaatgtta agggtgaagc atattctgac gatagtatga
     6421 ctatgggatt agttttacaa aaataataat ttttttaaaa tgaataaata aaaacttgaa
     6481 acctttaaca ataaaataac ataagaaaag cttacatatt gaaaaatgga aacttttatg
     6541 ttatttttag tacattacaa atttgtttat ataaaaatca atacttgaat tattattttt
     6601 atgtgaaatt tatctatagt atatttttat aaacaagtaa tgttattaaa attaagtata
     6661 tagttaaaag tgtatagcaa aaaggctgtt atattaatgt tagaggttca gggaaaatat
     6721 ataaagcaaa gatatttaca gataatgtag attgcacagc tataagttag ataatagaat
     6781 tatgtaatca agaatttaca acaaagtata agataaggat aatgctaaat tgccatacag
     6841 gtgctagatg tgtaaatgga tttaattaat actattgata aagtgcaaca agcacataaa
     6901 ccaattgatg agattattga aactataaga gatatagttg acataattaa aatattggaa
     6961 cctgtttata attttaaatc aaatcaataa tgattaagat taaaataata ttaaggcatc
     7021 aatgttataa gagtacaata taaggagtac tatatagtac tccttactta tacatattag
     7081 gcaataggga atttttttat agttaaattt tcaaatattt tttagggaac tttataattg
     7141 agaattaagg gtatagtatg tgatgaactt actaaattta taattaggtt atataatgga
     7201 ataatcatca atagttttac tttagggaaa atatatgctt aaggaagtat agcttactaa
     7261 tattattaaa atgtatattt aaaatgataa aaaaagaatt agacaagtat ttagtaaatt
     7321 gactatgcaa aattctatca agcaggaaca ctatgacttt agtcatggga ggttcagtag
     7381 gtaacttgtt gaattaagtt tatattattt aaataaacta cttaatttag ataatataaa
     7441 aagtcagaat tttaagaata gagctatata atcttagcta ttaggagaag tactatgata
     7501 ggaatatatt ttagtggaac aggaaattca agatactgtg tagaaaaatt tttacaagaa
     7561 tatgatataa cagcaaattc ttattctatt gaagataatg aacttttaca gcacatcaat
     7621 aatcatgaga atattatttt tagttatcct gtgcaatata gtaatgtgcc aaaaatattg
     7681 aaagatttta taataaatca ttcaactttg tggaatggta aacgtgtttt tattattgca
     7741 actatggctc tatttagtgg agatggctct ggtgtgttag ctagaatatt aaaaaaatac
     7801 ggtgctataa ctgttggtgg attacatgtg aagatgccag atagtatagg agatgaaaag
     7861 gtattaaaac attctttaga gtataataga aagcttgtaa taaaagcaga aaagaaagta
     7921 actaaagctg ctaaaaaact gaaacatggg tcaccaccaa aagaagggtt gggttttttg
     7981 agtcatattg tagggttatt tggacaacga ttgtatttta tcaataaaac aaatatgtat
     8041 acagataaac taaaaattaa tactgaggaa tgtattgtat gtggtaaatg cgttaattta
     8101 tgtccaatga agaatctagt aattaaaaat tgtatgattg ttgcaaatgg gcaatgtaca
     8161 atgtgttatc gttgtgtaaa taattgtgcc aaaaaagcaa ttacattgtt aggtaaaaaa
     8221 gttgttgaac agagtaatat taaaaaatat atgtaagaaa tttaaagttt tattgaatgt
     8281 aaattgatgt atattttatg ttagaaagag aggttaaata atggaatact acatagttga
     8341 ttcatttgca actaaactat ttaaaggaaa tcctgctgga gtttgtgttc ttgacagaag
     8401 aataccttta gagttaatgc agaaaatagc agaagaaaac aatttaccag aaacagcatt
     8461 tgtagttaaa tgtaaaggaa attatgaatt acgatggttt actcctaaag cagaaattga
     8521 tttatgtggt catgcaactt tagcagcagc ttatgtaata tcaaatttta ttgatgttaa
     8581 tgtaaaaagg atagatttct ttactcaaag tggaaagctt gaagttacac gaaatggaaa
     8641 tctatatgaa atgatatttc cagaaataat gcctattgaa atagagttat caccacaaca
     8701 agctaatttg ataggctgtg taccaagtgc tgtttattct tcaagagatt taatcttatt
     8761 gctaaacagt gaacaagaag ttataaatta taaacctaat tacgcacaat tgcgtaaatt
     8821 gacagactgg ttaggtataa taatcacagc acaaggaagt aatacagatt ttgtatcaag
     8881 atatttttgt ccagaattag attcagaaga ccctgttact ggttcatcac attgtaatct
     8941 tattccatac tggtctgaaa agttgggaaa acacaaaatg gtagctgctc aactttctaa
     9001 tcgtggtggt ataattcagt gtgaagtgtt gaaggataat actgtaaaaa tttctggtga
     9061 agcagtcttg tttatgcagg gaacaataaa gattgatatt taaataagta taataactaa
     9121 tttattatga aaaataatta attttcttag tgttaacaat aataaagtag agttaaaaag
     9181 cttgatttca cttaaatcaa tatgatagat gttgatacta agattgacaa gcatgattaa
     9241 aaaagataat atttaatcta taaagataaa aacacagtct ggttggtagt ccagacgcag
     9301 caatagctgt cagtaacctg cctccttggt tgtccgttct ttagaaagta atttaaggag
     9361 gaactagcaa tggacaaaat tagtggaaaa ttaacagttt tatttgaaga accattttgg
     9421 gtaggtatat ttgaaagaca agatggtaaa aaatatgaag catgtagggt agtttttgga
     9481 gcagaaccta aagaagtaga agtgtatgaa tttatacttg aaagattttt ctcattagat
     9541 tttggtagca ttaaattaga aaaaaatgtg accaaagaca acattggata caaaagaatg
     9601 cagagaaaag taaaaaagga acaagaaaaa gaaactatag ggactaaggc tcaaaatgct
     9661 cttaagcttc aatatgaaga aagaaaacag gatagaaaaa acctagctaa aaatagaaaa
     9721 gaagaagaaa aagaaagact atttaatttg aaacaagaaa agagaaaagc taaacataag
     9781 ggtcattaat tttaattaaa tatcttttta ataattgcta gaaaaaggtg tataaaattt
     9841 ttaattttat acaccttaat ttattgaaaa gaataaggtt ttatatttca attactttaa
     9901 aatataaaaa tatactaata aaaattgtaa aaatagtata aatggtatac caaatgtaaa
     9961 tttaatgtgt ttagttttat gtctaaaaga atacattcca attatagaac ctatactacc
    10021 tccaattact gctatagaca taagagtagc ttcttttatt ctccattcgt ttctaattgc
    10081 tcttttctta tctatgtaca ttgaaaagaa agcaatagaa ttaatcacta taaaatatat
    10141 tagaataaaa tttttcacag tcttgcctcc ataaaaatat attatacatt ataaaacaag
    10201 atatttgttt tggaaagtta aatttgtaat tagtacatat aaaatatttg ataagaacaa
    10261 atataatatt aattggaaaa tttaaaaaaa ttaaacaatt agcaaatagg gggtatattt
    10321 ataagacaca aagtgataaa atctatttag aaaataatgt aaaataacag gaggataaag
    10381 caaaatgaaa tttatagtag aaaaagaagt ttttgataaa ttagaaaatg tatgctttgg
    10441 tgtagtagtt gcaaaaggaa tagataatac taaagaaata gaaagaataa gcaacctttt
    10501 agatataagt atagatagag tagaagatta cttcaaagat aaaaaagtaa aggaatcaga
    10561 agaaattata ccatatagag aggcatttag aagtctaggc atgaacccaa ataaatttat
    10621 gagttcgata gaagcaatga caacaagggt tgctaagaat aagaaacttc ctcatataaa
    10681 tcctattgta gatttaggaa actctatttc tttaaaatat ttacttccaa tgggtgctca
    10741 tgatatggat tttagaaatg atgatgtata tgttagattt tctaaaaaag gggataaatt
    10801 tgtaccattt ggagaaactg atgttgaatt aatggaagaa ggagaattaa tttattcagt
    10861 tggagacatg gtaaaaacta gaagatggat atggagacaa ggagaggaag gaaaaattac
    10921 taatagctca aaaaatatat tttttcctat agatggtttt actgatgcta atttagataa
    10981 ggtaatgagt gcaagagaag aattagcaaa attgttaaag gaaatattta attgtgaaat
    11041 taaagtaggt tttgtagata aagataatcc tgaaatggaa atataataag ttgttgaata
    11101 cttgttaaga tattatattt acttaatgat gttaaaaagg tattaaaaaa gaaaggtcgt
    11161 agtaaatttg aaaaaaacta taataaagaa tgtaaatcca atgattgaaa ataagtttat
    11221 aggacttttt gaaatagagt ataaaaacaa attagatgaa gataaggttt ggatggtagc
    11281 atcaagaaaa agtagtgagc aattaaaaaa tatttatttg aagaatgaag aagatagtgt
    11341 agatgcagtt gtaatagttg ggcttcataa aagtagtaaa aaattaattt taataagaca
    11401 atttagagta cctattaatg gatatatata cgagttacca gcaggtcttg ttgatgaagg
    11461 agagtctata gatatttccg ttgaaagaga acttagagaa gaaacaggac ttactctttt
    11521 agaaattaaa aaaaataaaa gtattgataa agtttattta tctcctggaa tgagtgatga
    11581 atctgttgcc tttgtttatt gtatatgtga tggtgatata acagatgaat ttttagaacc
    11641 agatgaagag attgaagcgc ttttaatttc gcaagaagat gcaaaagaga ttttacaaag
    11701 taatcataag atagacacaa aagcattttt gattttacaa atgtttgtga gtttaggagc
    11761 taaattattt gaatagatat atagaaaaga ggttatatag aagagtaaaa taataaagtt
    11821 tattttactt gtataaatta taatctcttt ttttatacaa taaagtataa ttttgtgtat
    11881 ttttaaataa gtgacttgac tcttaaattt tcataatatg ttataaacca atttaagata
    11941 ctacaataat taaaattcca aaattattta acatatttgc aattaaaatg agaaaaggta
    12001 aaggttgaat gattgaaaaa tttgatgtaa aatagtagct tagtttaata ttatagtttg
    12061 tagggttaaa aattagaaat tgtaagggag ataaattcta tgaaaaaatt agttattatt
    12121 ttaattagta tcattagtat ggttagcttg acagcttgta aaaaaagcaa tgaaagtaaa
    12181 tataataatt taaacaacaa agaatccgga caaacaacca ttgaaatgga cttagataaa
    12241 aattatgata cttcagaccc atttgttaat gcacgtttgt tttgtgtgtc taatgatata
    12301 gatgttttag acacagagat ttcttttaaa atggatggag atagtggtat tgtggaaata
    12361 aaggacaata aaacatatga aactttatgg agtaatacat ggcatggaag cgttgataat
    12421 gatacattca caatttctct ttctaatata caaaaagaga aagagtatgc tatatgcttt
    12481 actggaatta aaataaatca tgcagttgta aaggttactt ttgaaagcaa tttagttaag
    12541 gagaaagaaa aaccctcaaa ataaattcca gattgtctat aacttgatat tcagtacata
    12601 aaagcagttg atatttatga ttgactgctt tttcttatca aaaaggagag tttgtgcatt
    12661 tttacttgaa atagtttatg cacatcaaaa gttggtgagc atgatattgg aaatggtaag
    12721 catgaaatct tgataagaca aggaaataaa tttaagtttt caattaagta tatttccata
    12781 atttagttaa caataagcta attattattt atattttgat atataaataa taattgtagc
    12841 aaatttcaaa aaatcatata ttttttgaac taaataaaaa tttattttta ttattttaaa
    12901 ttagaggaga ttcttgaaga tatattattt ttaaaaataa gtatagttta aaaattcaaa
    12961 aaaaagatgg caaaatatgt cccaagggac attgagtaaa ataaatgatt tcagcttatt
    13021 tacactaaaa agtacatact aaatgagggg taaaataaag aaaaatgata tactaattgt
    13081 cttttgataa taaaaaaaca tggaatttga ttatttaatc aagaaaaagt attgaacaaa
    13141 tgatttattt agtgtacaat aatcatgtaa aaacatagta atggagggag acatttcata
    13201 atgaaaatta aggtaggaat gaacggattt ggaagaatag gaagagctgt attaagaata
    13261 gctcaagaag aattaggaga taatatagaa attgtagcca taaatgcaag agcaacaaca
    13321 gaatctttgg cacatttatt tacatatgat tcatgctatg gaacttttag aggtgaagta
    13381 gaggcaaagg atgaagacac tctagtagta aactctaaag agataaaaat attaagatat
    13441 aatgacccag aagaattacc atggaaagaa ctgggggttg atatagtaat agaatcaaca
    13501 ggattattta cacaaagaga aaaggcagaa aagcatatta aagcaggagc aaaaaaagta
    13561 attataacag ctccaggaaa aaatgaagat ataactatag ttatcggagt taatgagaaa
    13621 gaatatgata atgaaaagca caatataatt tcaaatgcat cttgtactac aaattgtcta
    13681 gctccttttg ctaaagtatt ggacgaaaaa tttggtatag taaaagggtt aatgactaca
    13741 gtacattcat atactaacga tcaaagaata ttagataaaa gccataaaga tttgagaaga
    13801 gcaagagcag cggctgaatc tataatacct accactacag gagcagcaaa agctgtgtca
    13861 agagtactac cacaattaga aggaaaatta aatggttttt cacttagagt accaacacca
    13921 acagtttcac ttgttgattt agtgtgcgaa cttaaagaaa acgttacatc tgagcaagtt
    13981 aactctgtgt taaaagatgc tgctgaagga gaattaaaag gtgttttagg atattgtgac
    14041 aagccacttg tttctataga ttataaagga gattcaagat catctattat agacgctcta
    14101 tccacaatgg ttatagaaga caatatggtt aaggtagttt cttggtatga taatgaatgg
    14161 ggatattctt caagaactgt tgacttagta aaatatgtag ctgaaaaatt aaaataatat
    14221 atatttctta aagcaaattt taaaaaaatt ttttaaaaaa attatataaa actaaaaaaa
    14281 aagattatga aaaatattga aggaaaaatt ttttcataat cttttttctt tcatgcaaaa
    14341 agatacaagt tacaacttat atttgcatat taggtcgtta gagaagtata tatatattta
    14401 gtaaggaatc atacttttta taaaatgaaa gataagatgt cgaaaacttg cataaatctg
    14461 aaaaattttt attattaaaa tactgatttc cattttaaaa atgataaaaa aatgacaaat
    14521 aaagttgcaa atagtaatac ttataagtat aattaaaaaa gagaaaaggt tttgttaaac
    14581 tttataacaa aaaaagaagg gatgattcaa aatggagaag ttaacagaca ggagacaggt
    14641 taaggcagta agcatggaaa ccttcatttt cctaatatta ctagtagttg gttttgggta
    14701 tgttggtagt attatgggag caggaatgat gttcaaagta ataatgagta cagcacatgc
    14761 tttactattg gagacagttt tcttaattat ggctatggca gttcttgcag gcgcattaag
    14821 cgcactactt tcagagtttg gagtaatagc gttaattaat aaaattttcg ctgtatttat
    14881 gaagccttta tatggactac ctggtgctag tatagcagga gctataacaa cttatttatc
    14941 agataatcca gctattatac cttttgctaa agataaaaca ttcacacagt attttaaaca
    15001 ataccaagtt ccagcattat gtaacttagg aacagccttt ggtatgggac ttatcttaac
    15061 aacatttatg atttctcaag gtacagaata tgtacttcca gcattaattg gtaatttagg
    15121 tgcaattata ggtagtgtca taagtgttag aataatgttg acatttacta agaagtttta
    15181 taaatacagt ccggaagaag ataaagcaac aggaacatta gagaaaaagg atgaatttag
    15241 agaaatcaga gaaggaaatg tatttcaaag agctttagat gcaattcttg aaggtggtaa
    15301 aatgggggta gatatgggaa tggctataat tcctggagtc ctagtagtat gtacattggt
    15361 gatgttatta acttttggac catctacaga ccctgttaca ggtcaagaag tttatacagg
    15421 agctgcatat gaaggaataa aattacttcc agttattgga gataaacttg gatttatatt
    15481 agaaccatta tttggattta catcaccaga ggcaatagca ttccctataa cttcattggg
    15541 agctgttggc gcagcaatgt ctctagttcc agagtttata aagtcaggag ctataactcc
    15601 taatgatata gcagtattta cagctatggg tatgtgttgg agtggatatt taagtacaca
    15661 tattggtatg atggatgcat taaatgctag acagttggca ggtaaagcta ttctttctca
    15721 tacaataggt ggtttatgtg caggagcagc tgctcacttt atatttacac tcgtaggata
    15781 aatgtaatca gctaagataa aaaataatat acaaaaaatg gttgttgata tgtaataaga
    15841 atttacatat caacaaccat ttttttctga ctaaagataa tttataatac ataaacatta
    15901 agtggatatt agagttaagt tatatttatg taaaaagtaa caaaaatctg tagaaattta
    15961 tgacaaaatt tagtacacta actatatgtt cttatttaaa atttaaggtt agaattagga
    16021 attacctagt agagtaagct ttgattaata aaagaatcat aaaataaaaa tattagtcat
    16081 aaagattata aataacttac ttaaaataaa gccatagaac ttacaaaaat taaaaaagca
    16141 tataaatatg gaggggtaca atggatgaat tatttcatat aggaattgat gtaggttcta
    16201 caacggtgaa ggttgtggta cttaataata gtaataatat agttcataaa gaatatagaa
    16261 gacattattc tgatgtaaaa aaatcagtta aagaagtact aaatggtata tatgaaaaat
    16321 tgggagatat aaatactacc attataataa ctggttcagg tggaattgga atatcaaaaa
    16381 aattaggtgt aaaatttgtt caggaagtta tatctagtac aaaatcaata gagtattttc
    16441 atcctgagac tgatgttgtc atagaacttg ggggtgaaga tgcaaagata acatatctaa
    16501 gtggaggaat agaccaaaga atgaatggaa tatgtgcagg tggcactgga gcattcatag
    16561 accagatggc atcactctta aagacagatg ccagtggatt aaatgaactt gctaaagggt
    16621 ataatgttat atatcccata gcttcaagat gtggtgtttt tgctaaaaca gatattcaac
    16681 ctcttataaa tgatggagcc aatcagactg atatagcaat gagtattttc aatgcagttg
    16741 tcgtacaaac tgtgagtgtc ctttcttgtg gaagaaaaat agaaggaaat gttgcgtttt
    16801 tgggtggtcc attgtacttt ttatctgaac ttagagaagc atttaaaaga gtattagatt
    16861 taaaagatga aaacataatc ttcccagaaa atgcacaact ttatattgct attggagcag
    16921 gattattatc agtagatgaa gataatatac aactaaaatc tctaatagaa aaattggata
    16981 gtataaagaa tattgaagat ggagaagtaa atttattaga acctttattt aaagataaaa
    17041 atgagtatga agaattttca agaagacatg aaaaagaaaa aataaattat gtggacataa
    17101 attcaataaa aagcaattgt tatttaggaa ttgatgcagg ctcaactaca actaaagcag
    17161 cacttattga tgaggatgga agacttgtat attcttatta taatagcaat gaaggaaatc
    17221 cattaaaaac aaccattaaa gttataaatg aagtatatga tatattacca aaaaatatta
    17281 aaatattgag ttcaactgta actggatatg gagaaggtct tattaaaaaa gctcttaaaa
    17341 tcgataatgg agagatagag actatagcac attacaaagc tgctaaattt ttcaacaaag
    17401 atgtagattt tatacttgat attggtggtc aagatatgaa gtgcctaaaa ataaaagatg
    17461 gagtaattga taatataata ttaaatgagg cttgttcatc aggttgtggc tcatttttag
    17521 agacatttgc aagttcttta tctatgacaa tagaagaatt tgcgcatgaa ggtatttatt
    17581 ctcaaaatcc tgtagattta ggttcaagat gtactgtatt tatgaattct agagtaaaac
    17641 aagctcaaaa agaaggtgca agtgttggag atatatcagc agggctatct tattcagtaa
    17701 taaaaaatgc tttatttaaa gtaataaaga ttagagattt aaatgagatt ggtaataata
    17761 ttgttgtaca aggtggaacc ttttataatg atttggtgct tagaagcttt gaaaaactaa
    17821 ttggaaaaaa tgtaattaga cctaatattt caggaattat gggagctttt ggttcagcac
    17881 ttatagctaa agaaaaatat gaaagtggat acaaaacatc cttgttatct aaggaagagt
    17941 tgaataatat caagctagaa gcaagtgtaa ctagatgtaa aggttgttca aatcattgtc
    18001 ttcttacaat aaataaattt tctgataatg aaatttttat ttcaggtaat agatgtgaga
    18061 agggtgaagc tatttatggt gataaaaaaa taggattaga aaagaaaaaa ccaataaatt
    18121 tatttaagta caaatacaat agaattttta gatacaaacc attggaaaaa gaagaagcaa
    18181 aaaatgggga aatagggata ccaagagtat taaatatgta tgaagattat ccattttggt
    18241 ttactttttt caatgctatg ggctttagag ttgtactttc agataggtca tcaaaacagt
    18301 tatatgaaac tggtataact agtattgcat ctgaaacagt ttgttatcca ggaaaattag
    18361 ttcatggaca tatagaaaat ttaattgcaa aaggtataaa aaatatattt tatccaagtg
    18421 taacaaatga aaataaagag gattcaaatg cagataatta ttataattgt cctgttgtaa
    18481 tatcatattc tgaagtaata aaaaacaatg tagagaatat aagaaataag aacataaatt
    18541 atataaatcc atttataagt ctaaatgata aggaaaaatt gaagaaaaga ctctatgatg
    18601 aattatcaag acatttttct gatataaaaa taactaaaga agaagttaat catgcagttg
    18661 ataaagctac tgatgaacag aatttattta aaatggagat acaagcagca ggtgaaagag
    18721 cacttaaaga aataaaagaa agaaatatga aggggattgt gttatgtgga agaccatatc
    18781 atatagaccc agaaataaat catggaatgc cagagcttat aaattcatta gatatggcag
    18841 tgcttactga agactctata tgtcatttag ccaatgtaca aagaccactt agagttgtag
    18901 accaatgggt atatcattca agattgtata aagcagctag ttttgtaaga gataaaaact
    18961 atttggaact tgttcaactt aattcatttg gctgtggatt agatgcagta actacagacc
    19021 aggttcaaga aatacttaat gaaaaatcga agatatatac tattataaag attgacgaag
    19081 gcaacaattt aggagcagct aagataagga ggaggtcctt aaaagcagct atgtttgaga
    19141 gagaaagcaa aaatatagat gtaaaaaata taaaggtaaa caggatacag tatgctaaaa
    19201 ataatcagat aacgaacaaa catactatat tagcaccaca aatgtctcca atacatttcc
    19261 aattccttga aaaagctgtt aacttaagtg gttataatat agaaattcta aaagatactg
    19321 atagtagtgt aattgaagaa gggttaagat atgtaaataa tgatgcatgt tatccagcta
    19381 taatagttgt aggtcagcta ataacagcat taaaatctgg caaatatgat ttaaataaca
    19441 cttctgttac tataactcag actggtggag gttgtagagc tacaaattat attggattct
    19501 taagaaaggc tatatacgat gctggattta aagatgttcc tgtcattgca cttagtgtaa
    19561 atggaataga agatagtggt ataatggata atatatcact aaaattaatc aatagactct
    19621 ttatgtctgt tgtttatggt gatttgctga tgaaagtact atatagagtt agaccatatg
    19681 aaaaagttac tggaagtgca aatgctctat atgaaaaatg ggttgatata tgcaaatctt
    19741 ctcttgttaa agcaaaaatt tctgtattta ctagaaatat aaaagatata gtaaaagaat
    19801 ttgataattt agagatacta gatatcaaaa aaccaaaagt aggtttggtt ggagaaatat
    19861 tggtgaaatt ccatccaatt gcaaataata atttagtaga tatattagag cgagaaggtg
    19921 ctgaagcagt cgtgccagac cttacaaatt tcttcttaag ttgtgccttt aatactatat
    19981 acaaacacac tcatttagaa ggaagtagaa agagtagaat gataggagag gcatttatat
    20041 acataactgg aatatatcaa agagtatata aaaaagcact tgataagagt gagagatttt
    20101 atgctccagc caatataaaa aatgttgcta aaagtacaga accagtagtg tcattaggta
    20161 atcaaactgg agaaggttgg cttttaacag gtgaaatggt tgaattatta aatgaaggtg
    20221 tagaaaacat tatttgtatg caaccttttg gatgtttacc taatcacata attggtaagg
    20281 gttctataaa agaattaaag aggttgtata aaaatgcaaa tataatacct attgattatg
    20341 acccttctgc aagtgaggta aatcaactta acaggataaa acttatgctt tctaaggctt
    20401 ttaaaaatat ataagattta tattttgata tgttaaaagt taatgaattt aaaaatatac
    20461 tctatattaa gtatctataa aaaagagtta tcctattttt tagggtaact ctttattagt
    20521 taaaaaatta tatgtatact gatattaaag gggggaagta atatgagttt agtatatgat
    20581 gtttataaat caaaattagg aaatttgtac atattaagtg aagatggttt tatagttagt
    20641 atatacatag gagatgaaaa atttaataat ttaaaaaatg aatgtattaa aaaaggaatt
    20701 gatataaaga gaagtaagga agatttaaag gaagcaataa gccagataga tgattacttt
    20761 aatggaaaaa gaaaagtctt taatttaaat attcgtattg aagggactga atttcaaaaa
    20821 tcagtttata gggaaatgtt aaaaatacct tatggagaaa cattgtgtta ttcagatata
    20881 gcaagaaata taaacaatcc aaaatcagta agagcaatag gacaagcaag taaaagtaat
    20941 aaaattccaa taataatacc ttgtcataga gtagttggca aaaaagatat tggtggatat
    21001 atggggaatc attctgattt gaaagagata ctattaaatt tagaaagaca gagttaactt
    21061 agaaagaaat gtttttattg ttaatatttg atataataat agtaaaaaga agtatttttt
    21121 acaaaaacaa tgaagaattt attttaattt acaaaaatga gggggaattt taatgtgtga
    21181 taaagttata gcatcaaaat atcatgagaa gggatttaat tgtgcagaat cagttataaa
    21241 agcatataat gaggaattta atacagatat accagtttgt ttaggaagtg gactgggttc
    21301 agggtgtggg gttgctagtt tatgtggtgc agtaaatgca tcaaatataa taattggata
    21361 tgtaaaaggc agaaaccacg aagatgaaag tactaaagct aaagtatatg ctaaagattt
    21421 aactacaaca gtaagaaaag agtatggttc agagttatgt atagacctaa aaaaagattt
    21481 agttgcttgt agagaaataa tggatttcgc atatgatagt ctaaaggaaa ctttaaaaaa
    21541 agaattataa gaaaataagc tatcaaatat aaattatttg atagcttatt tttatagccg
    21601 atactaaata aatcaaatat attttagaat tattatttgt aaataaacag caaataattt
    21661 taggcttttt atttaatata gtttttattt atatttattt ttgaatatgt tatactttgt
    21721 ttaaataaat atttgtaatg agggagatga gaagattgaa gaatctcaat ttacttatta
    21781 ataaattata ttcaaaaaat cataatgaag catataaaac ttttttattc ttagaaaatg
    21841 aaagtttaaa atcaaatatt acatattgtt tttttgactc attcctagaa atgattgata
    21901 acgaaaactc atatattcga acaaggggat tgttattaat ctctgcaaat gctcagtggg
    21961 atattgataa taaaattgaa ataaatatag atagtatact aagtcatatc gtagataaaa
    22021 aaccttctgt atcaagaatg tttattaaat ccatacctaa tattacaaag tataaagaaa
    22081 atcttattaa tcgcataaaa atggaattaa gtaatgcaga tataagtatt tataataata
    22141 atatgaaacc attagtagaa aaggatataa acgatacatt atctaatatt agttaaatat
    22201 taa
//
LOCUS       DAELTA010000051        22141 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.051, whole genome shotgun sequence.
ACCESSION   DAELTA010000051 DAELTA010000000
VERSION     DAELTA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.051"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            491..3133
                     /gene="adhE"
                     /locus_tag="KPG13_001679"
                     /gene_synonym="adhC"
     CDS             491..3133
                     /gene="adhE"
                     /locus_tag="KPG13_001679"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG0985412.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3493..3834)
                     /locus_tag="KPG13_001680"
     CDS             complement(3493..3834)
                     /locus_tag="KPG13_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985413.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3861..4532)
                     /locus_tag="KPG13_001681"
     CDS             complement(3861..4532)
                     /locus_tag="KPG13_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985414.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4544..7120)
                     /locus_tag="KPG13_001682"
     CDS             complement(4544..7120)
                     /locus_tag="KPG13_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0985415.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7328..8263
                     /locus_tag="KPG13_001683"
     CDS             7328..8263
                     /locus_tag="KPG13_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0985416.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8282..9019
                     /locus_tag="KPG13_001684"
     CDS             8282..9019
                     /locus_tag="KPG13_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985417.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(9204..14261)
                     /locus_tag="KPG13_001685"
     CDS             complement(9204..14261)
                     /locus_tag="KPG13_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG0985418.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14286..14705)
                     /locus_tag="KPG13_001686"
     CDS             complement(14286..14705)
                     /locus_tag="KPG13_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985419.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14939..15190)
                     /locus_tag="KPG13_001687"
     CDS             complement(14939..15190)
                     /locus_tag="KPG13_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985420.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15279..16010
                     /locus_tag="KPG13_001688"
     CDS             15279..16010
                     /locus_tag="KPG13_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG0985421.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            16069..16980
                     /locus_tag="KPG13_001689"
     CDS             16069..16980
                     /locus_tag="KPG13_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0985422.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            17031..17843
                     /locus_tag="KPG13_001690"
     CDS             17031..17843
                     /locus_tag="KPG13_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG0985423.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17952..18644
                     /locus_tag="KPG13_001691"
     CDS             17952..18644
                     /locus_tag="KPG13_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0985424.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18731..19672
                     /locus_tag="KPG13_001692"
     CDS             18731..19672
                     /locus_tag="KPG13_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985425.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19709..20866
                     /locus_tag="KPG13_001693"
     CDS             19709..20866
                     /locus_tag="KPG13_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0985426.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            21018..21578
                     /locus_tag="KPG13_001694"
     CDS             21018..21578
                     /locus_tag="KPG13_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0985427.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21606..22097
                     /locus_tag="KPG13_001695"
     CDS             21606..22097
                     /locus_tag="KPG13_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985428.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
BASE COUNT         8241 a         2744 c         3359 g         7797 t
ORIGIN      
        1 taaaaataac ttatagagat tagaataatt ttagtttata tattttttaa aactatacag
       61 ttgttataaa aagaaaaatt gcatacaagt caaaaataag tgttatttta taaaaatacg
      121 acaaaaattt tatttactac ttatacaaat aacatgcaaa taacatataa aatttaataa
      181 gtaaaaaaaa tgaaagatat aaattatcat tttagtattt tatttagtcg aagtaaatta
      241 ttcaaatgaa atatatctca tttagataaa aaattttagt aaaagtaaaa aaaataatat
      301 atttgttaaa aaataaattg acaaaatatt cagaaaaaaa cttcttgacc taagagagta
      361 aaataaataa aatctaaggt atagagatga aatctataaa attattttaa gtataagtta
      421 aaaatataga tgatgcattt aagatttata aaaattagag atgcataatt attttaggga
      481 ggtttttagt atggagaaaa aagaaaaggt agtagaaaaa tcaaatgttg aagtatgtag
      541 tcctgaaatt gtcaacagtg ttgaaacatt aagaatgaga ttagaagaaa ttagattggc
      601 acaaaaagaa tttgcaacat ttacgcaaga acaggtagat aagattttct tagctgcttc
      661 aactgcagct aatcaacaaa gaatacctct agcaaaaatg gcagtggaag agactggtat
      721 gggtattgtt gaagataagg ttataaaaaa ccattttgcc tcagaatata tctataatgc
      781 ttataaagat actaaaactt gtggtgtcat agaaaaagat gaagcatttg gttttacaag
      841 aattgctgag ccagtaggtg ttttatcagc agttatacca acaactaatc ctacatcaac
      901 tgcaatattc aaatccctaa tagcactaaa aactagaaat ggtataattt tctctcctca
      961 tccaagagct aaaaactgta ctatagaagc agctagagtt gtacatgatg cagcagttaa
     1021 ggcaggagca ccaaaaggac ttataggttg ggtagatgtt ccttcgattg aattaactaa
     1081 tgttgtcatg gcagaggcag accttatatt agctactggt ggacctggaa tggttaaatc
     1141 tgcatactct tcaggtaaac cagctgtcgg agttgggcct ggaaatgtac ctgcaataat
     1201 agatgagagt gcagatataa aaatggctgt aagttcaatc ttagtatcaa aatcattcga
     1261 taatggtatg atatgtgctt ccgagcaagc tgtaatagta ccagaaaaaa tatatgaaga
     1321 agttaaaaaa gaatttaaat atcgtggagc acatttccta aataaagaag aaactgaaaa
     1381 agtaggaaaa gtagtaataa ttgatggttc attaaatgcc agaatagttg gacaaccagc
     1441 acatgtaata gccaaaatgg cggatgtaga agttccaaaa acagctagaa taataattgg
     1501 tgaagtagaa tctgttgaat taaatgaacc ttttgcacat gaaaaacttt ctcctgtact
     1561 tgcaatgtac aagtctaaaa gttttgaaga tgcagttgct aaagcagaaa aattagttgc
     1621 agatggaggc tatggtcata catcttcatt atatgcagac tctataaatc atccagagag
     1681 agtagaaaag tttgtaaatg ctatgaagac ttgtagagta ttagttaata ctccatcatc
     1741 acaaggtggt ataggtgatt tatataactt taaattggca ccgtctctta ctcttggttg
     1801 tggttcttgg ggtggaaact cagtatctga aaacgttgga gtaaaacatt tgcttaatat
     1861 caagacagta gctgaaagga gagagaatat gctttggttc agagcaccag aaaaggttta
     1921 tttcaaaaaa ggatgtttag gtgtagcagc aagagaattt aaggatgtta tggataaaaa
     1981 gaaagcattt atagtaacag attctttcct atataataat ggatatacta agaaattaac
     2041 tgatttatta gatgagatgg gaattaagca tactacattc tttgatgtag ctccagaccc
     2101 aacattagct tgtgcaagag aaggagctaa agctatggct gatttccaac cagatttaat
     2161 aatagctgta ggtggaggaa gtgcaatgga tgctggtaag ataatgtggg taatgtatga
     2221 gcatccagaa gtagatttcc aagacttagc tatgagattt atggatataa gaaaaagagt
     2281 atatgtattt cctaagatgg gtgaaaaagc gtattttgct gctatcccaa cttcagcagg
     2341 aactggttca gaagtaactc catttgcagt tataactgac caagattcag gtgtaaagta
     2401 cccattagca gattatgaat taatgccaaa catggctata attgatgctg atatgatgat
     2461 ggagatgcca ccaagattaa cagcagcatc aggtgttgat gctttaacac atgcattaga
     2521 agcatatgta tctatgttaa gaactgaacc agctgacggt ctagcactac aagcaggtaa
     2581 aataatattt gaatatttac ctcgtgcata taaaaatgga aagaatgata aagaagcaag
     2641 agaaaaaatg gcaatggcat ctactatggc aggaatgtct tttgcaaatg ccttcttagg
     2701 aatatgtcac tctttagcac ataaattagg tgcattccac catgttcaac acggtgttgc
     2761 aaatgcatta ttaataaatg aagtaatcaa gttcaattgt gcagaagctc caaataagat
     2821 gggtgcattc tcacaataca gatatcctga ttgcatacaa agatatgctg aatttgcttc
     2881 ttttgcaggt attaaaggaa gtacagacca agaaaaagta gataatctta taaaagcaat
     2941 agatgaacta aaggcaaaag taggtcttcc taagactata aaagaggctg gagtagaaga
     3001 atctaaattc ttagaaagat tggatgctat ggttgaacaa gcttttgatg accaatgtac
     3061 aggtgctaat cctagatacc cattgatgag tgaattaaaa gagatatatc taaaagttta
     3121 ttatggtaaa taatatctat tgtgcaacat aggttctatt gggtttgtat ataaattatt
     3181 agatttgaat ataaatagag ttttattaga gacacgtaga agtaactata aataatagat
     3241 tataatttaa attctaaatg gaaatgataa aaagtcagta atttaatggt aatttcgaat
     3301 atgtgtattt aatcaattaa ctttaaaatt agtcggaaaa tttttttatt acatataatt
     3361 ttaaagcata atatataaaa aactttatga aaaaattata aaaatattca gaaaattcta
     3421 ttgactaatt tatttatcat gatataataa gtttagaaaa gaaaaaaatt ggggcctatt
     3481 aaggccccct tactattttt tgatagattt atttaaaatt actttctcat tttgattttt
     3541 tatatttatt atagagtcag tataatccac ttcaaagttg gcaaaagcag agaataaatc
     3601 tatatctctt aacaaatgaa ttgtcataca ttgattgttc ttatttagcc ttagaatcaa
     3661 tgctcttaca aatggaaatt gtattctaga attaaataag ttagaaccat cagtatctat
     3721 atctaagcta cgataattaa tatctataaa tatatctaca tcttcatttt cttctcttat
     3781 tattctgtag tcaaaatcca agtttaaatt taaattacct aaaatcaata acatttaaat
     3841 cctcttttct ataaatagtt ttagagtgtt aatttaagct ctccatctat tatagatata
     3901 attctatctg cttttctagc aatattttca ttatgtgtta ttattataag tgtttggtta
     3961 tattttttta tagaactttt tagtaaatcc atgacttctt ctgtagtttt actgtccaaa
     4021 tttcctgttg gttcatctgc taggattata cttggttttg cagataaagc acgtgcaata
     4081 gcaactcttt gctgttgacc accagataat tggttgggaa gatgattttt cctttcatta
     4141 agacccaata aatttataag gtcatcaata tattctttat ctattttttt attgtctaac
     4201 tttagtggta attctatatt ttcctttaca tttaatacag gaattaagtt atactgttga
     4261 aagacaaatc ctaccttttg tcttctatat cttgctaatt ctttatctga aagacaacat
     4321 atatctttac catctacctt tatagtacca ttacttggtc tttcaagacc acctactaaa
     4381 tgtagaaatg tacttttccc acttccactt ggtccaatta tagcaacaaa ttcgccttta
     4441 tttattttta aatttgcatt ctttagtgca tgtacagatg tttccttttt tccataaatc
     4501 ttacctaagt taatagtttc caatataggc ataattattc ctcctattct gtaatgttta
     4561 tagcttcaac tatactaatt ttatttatct ttcttgatgg aatatatgta gctagaatac
     4621 ctacaattat atttgctaca acaactagta tatatatttt gtaatctatt gaaaatccca
     4681 atccatatga tacaaagccc tgcatataat acattttaaa ttgtattata attcctgaca
     4741 cgatagtaat tatacttgag agtacaccat ataatattcc ttcataaagt atcatatttt
     4801 taaagcctct ctcagagata ccaatagctc ttaacattcc aaattcacta gttcttgaag
     4861 ttaagttata acttatgttg tttattatgt taagtacact gattatgaac attattgcta
     4921 ctataccata actataaatc atagttctag atgtcatctt atcagaattt tctttttcta
     4981 ccatcatatc tactgttgta gttccaggaa cttcacttcc aattttacct aaaaggttat
     5041 ttattttttt cttgtttgca ttttcattaa ggtctgcata aactaagtta tacttatcta
     5101 caccagttaa tttttttagg tattcgctac tcgttattac atctacacca tcatctccag
     5161 agtacatacc attatctgca aaaggataac ttataatagc tccaacttta aattcatatt
     5221 ctaagtattc ataattatct tttagacttt cataaagacc tgtatccatc ccaccctttg
     5281 gatatttgac ttttatagta tcacctactt ttatatctac agctggtgtt ccttcaccag
     5341 gagtaaaagt ctttgtgtat ttctgtgttt tagaaatttc tggtacatat acaatagcta
     5401 aattttcttt tttcattttt ttcaagttta tatttccact tactaagtat ttttctaatg
     5461 actttatcat ctcatcatta taacctttta gcttttgctt tagtgtatat ttgccagtag
     5521 tcttattttt tatgaacaga ggatttagct tataataagg agttgaattt atagcttcgt
     5581 agtaatctaa tctatttatt ttatttacct ctattcttgt gtttaagata ctagcagttt
     5641 tgactttgtc aattccatct atatttttta ttttatctat ctgattatca ttaaaccctt
     5701 ttatatcttt aaaagaagta ttgttaccaa tttcattgag ataaacattt gatagaataa
     5761 aatctgcatt catatatgtt tgttcttgag tagatttgtt ttgcacatct aaaaaagaat
     5821 atttataatt gtcttttatg acgattatac ctcctatact catagataat acaattataa
     5881 tgaacccctt tttatttcta aagatgtttt tcgcagatat agattttgtt actgacatat
     5941 ttaaagttaa ttttgatatt aaataattaa ctttggttac atttttatca gctccaaaag
     6001 tttttctgat agcatcaata ggagaaattc ttcttatttt taagtatgta aagaaactta
     6061 taattagtat tattagtatt gtacatgcaa ttgataagag aattatttta tctggaataa
     6121 caaaaggtgt aacatttata tttccttcaa attgaatatt tcctacagac ctattaaata
     6181 tttgtgcacc tcccatacct atacatatac ctataggcat tgctattaga gcaagtatga
     6241 aaagttcata aagcataagt ttgaaaatcc taaagttggt ggaaccaata gctcttaaga
     6301 cgccatattc ctgtactctt tggtatattg atataacata gatactatat attacaatac
     6361 cagcaaatag actcatagca attgcaacgt atttacttga attatctaaa gtaccgtttt
     6421 tcatagtaga ttctataagc attgagtttc ttcctatact tttctcatct aacatagcat
     6481 tttttattat attgtctatg tttgtattta tatcactgcc ttcattaaat tcaatataag
     6541 ttgtaaaatc attcagttct gattcattga gattagaaaa aatttcacat tgtccctttt
     6601 ttttctctat aaatctatct tcaaggattc caacaacttt aaaagtttct ggattttctt
     6661 tttgatataa tttaaaagta aggttttgat ttatctctgg ttttaagccc atactattta
     6721 aaatccattt ctcgactacc acctcattgc tagcttttgg aaatcgacct tttataagtt
     6781 tagaatgact agtcaaataa tttttatttg tatatagtat gtttataggt aatttttcat
     6841 taacatctgt agacgcatag taagatgtta tacccagatt tttaatatct ttgctagatt
     6901 ttatggactt taattgattt ttctttatat ttttaaagta tacatggtca gttccaagct
     6961 ctcttttcaa tctatctaca tctgcttgct gagcacttct tgagagagta ccaataccaa
     7021 caacgagtga tgtagcaaga attatactca caacaattgc taaagaccta gctttataac
     7081 tttttatata ccttagtact attttcaaat tatctttcat atttaccact tcctttctta
     7141 actaaatcaa ttatatattt atctaaaata tatagcaaag taagtatttt caaattttaa
     7201 gtagttaatt gtacaaattc atctgtattt ttacaagttt aattaatata tatgtaaatt
     7261 ttaacttttt acataatttt tttaaaatga tataatccct atataaattg tagaggggag
     7321 gggaatcatg gataacaatt ttgtatttcc tatagtaata ctaactttgt ttatatttag
     7381 caatcttata atgaataagt ataaatttta caaagaatta gacatattaa aaagaggcat
     7441 attaacattt tttatatcgt gttttgcatt gattttattt atggagggca ctaaatatat
     7501 acaattaagg ttaggatata gttttttact tgaaggagta ttaaatatta tacttttttt
     7561 ggttttaatt attttaagtt tttatacagg ctttaaagtt ataagattga taaataataa
     7621 taatgaacag gaatataaat tacaaaaact agatagtaaa ataaatcata aggataatat
     7681 aattatcaaa ttaagaagtc aaaaacatga ctatttgaaa catttgcaag tagtgtattc
     7741 tctattaaat actgggctag atgaagatgc caaagaatat ataatgaata tatcaaatag
     7801 ctttaaatca aaaaattcaa ggtatggaaa agtatcttat ttagatgcta tagcttcatt
     7861 aaaatacgag gaatgtcttg aaaaggatat atcttttgat gtctacattc aagattttat
     7921 ggataattta gagattgaac ctaatgatat gagttcagta ctattaaaca taatagataa
     7981 tgcaatagag tctttgtcta ctataaaaaa agaaaagaag tatataagat tacatgcata
     8041 tgaagatgaa tttcagtata ttatagctat aaaaaataat ggtcctaaaa tacataatgt
     8101 tgaaagtata ttttggaatg gatttagtac taaagaagga caagataggg gatatgggct
     8161 acatttagtt aaaaatatat tagataagta tgattatagt atacttgtaa atagtgatga
     8221 ttttttaact gaatttgtaa tcttaatccc aaaatattat tagttaggag gagaaaagtc
     8281 tatgataaaa gttttgattt gtgatgatga taagattgta agagatgata tagcaaatat
     8341 agctaaagag tctggatatg tagattatgt taaaaaagtt aaggatggta tagaggcaat
     8401 tgaatttata aaaaatgaga aaatagattt attgattatt gacattgata tgccatataa
     8461 aaatgggatt gattctgctg aagaaattct ttctatagat aaggatatac atatagtatt
     8521 tgtgacgggt ttcgctgatt atagtcttga aagctttaaa gtgcatccta tagattttgt
     8581 agttaaacca tttaaaaaag aaaaaatact tgattcaatt aacatagcaa tagaccatat
     8641 taactcccat aagattgcta ataataattt tatagaagac accctttttg tatataaaat
     8701 aaggaagcag atacatatga ttaattttga tgatatagtt atgtttgaga aaaattcaag
     8761 agcaattaat ctacacacta aagatgaaga tataataaag ttttatgaga actttgatga
     8821 tttaaaaaaa cgcctacctc ctaatttctt tatgacccat agacaataca tagtaaatct
     8881 aagatttata cataaggtta tacctataaa taaatcttca ttagagataa ggtttataaa
     8941 ttttcaagaa aatgccatat taaacaaatc tctagaaaaa gattttttat atagatttta
     9001 tagggttaaa agattctagt gaagatagaa gaaaaatact ttttgtgtga tataataagt
     9061 accgtaaaga taatgtataa gagaagttta tataaaaatt atttgtaatt atgatttaat
     9121 ctatataaat tacagtatag tatatattgg actttctaat ttttttaaga aagtccaata
     9181 tattttagag atagttttta tacttataaa ttacttaaga tactagggtc tttaatctta
     9241 ctatcttcag caagtaatag tatcttagac ataatctcag cagttttagg gtcttcatca
     9301 gcgaatggaa ggaatatctt acctctactt tgagaatgta taggaagtat attcaaagca
     9361 cccatccctt ttttatgtac aataccagaa cccatatgca cagaatactc acctaaatta
     9421 cctgctataa gagcatgaga gccttcaaaa ttgacattgt taatttttaa taataataaa
     9481 agttctttag caattgctat acgcatctct acagtagaat ggctagtttc agggtcaaca
     9541 ccaccagcat gagcgacact tacaacaagg tctatatcac gcataacttc tgagaataga
     9601 attggaggta tagaagctaa ttctaaactt ttataagttt ttctatcaaa aaattctact
     9661 gtttcaatag taggaggctc tatatctgct ggtgaaaacc aatctgcaag tgcaaacatc
     9721 tttgccacta tattttcttt ataaaatact ttctgtaatc cctcttcata atcgacagtc
     9781 caaagctgat ttctaagaag tgctacagtt ttctttggct gaatttggtg accagcatat
     9841 ctgcgagata tagttttttc agataattca tcactgttta ttctataata ctcacggaat
     9901 acttgcttaa atggttgaac aatttgctta ctaaataaat catgttggaa ctcactccac
     9961 ttaccagatt caaacaaatg aactggatgt gcaagtttta atttattttt tgccgtcaac
    10021 ttataagttt caccttctat attgcaaata aagaacttag acttatcatt ataaacatat
    10081 ccaatattat catcacatac ccatactaga tttttaatca aagggcagat aactggattt
    10141 ttatagatat tggcaatttc tttaactgta aatactattt catttttcat agcttgttct
    10201 aaactctgtt ttgcacgaga atactgactc tttaaatctt tttggacaga tttaagttca
    10261 agtacatatg gatgtttagc aatagctttt ggtatactct ttaaagactt gttgcctttt
    10321 tgaactttag tagatgcttt tccgttttca tcaacatcta tccaaattga agtaccatca
    10381 atatcttttg gtttaaatag atgagcaact tcatttagtt tttcagtctc aagagcccac
    10441 atcatacgat ttacatctcc aaatccagca cttctagcta gattttctag cgctatagta
    10501 caagcttttg attcactttc acgacgttgt gcaccaaatt gcttgctttc tttagcaaac
    10561 ttttgaataa attcataacg gaaagttgct tcttttaggt ctcctttttt cataggaagt
    10621 aaagagaaac ttctcaattt atcttgattt ctatttgttt caatttcttt catcaacatg
    10681 tctttatcaa gcttaccaag tgtagcatca gcatagagtt gtgcacgtct atgagagtta
    10741 ccaccactag ttatatattt agctgacttg taaagtatat tgaatctttc ttccccaata
    10801 gttttatatg aatctaaaaa ccaatctaca tcaaatgcac catcaatgaa actacgtggt
    10861 gaaatagggg agaatagagc aacctctgat tctttttgtg cagagaagcc ttcatttata
    10921 tgtgcatgga agtaccaaat tgtacttttc agacccttcc agtttaaata tttttcaatt
    10981 atgtttaccc attgaggggc atacatagca gattctatca atcttaactc agtaatgtcg
    11041 gtatttttaa gtaaactttt taattttttt gaatcatctt ctttagtagg atagcaattt
    11101 ttaagtagag aacttaaaac atcttttttt gaataattgc taccatatga atatcctctc
    11161 tggaaagttt ctttaccaag agcgaccaaa atatttacaa aataatttat accttcaaag
    11221 tattgtatat tattagctag attagacact tctgttggca tatcacctct acgtgattca
    11281 atttctataa ttcggtttgt aacagttttt aaattatctt taagccaagg gtacttttct
    11341 aatgtgtcgc cagcccatct aggatttgtc aatgttctta ttatatttga tgcgttgtat
    11401 ccacttatca tttctctatt aacttgctct tcagtcatta atcctaattc atgtgcacga
    11461 gcaaacacat ttatgctagt atagaaagga aacttatatt ctagcaactg ataatttgaa
    11521 aacgtagtgt agaaaaactt agtaaaagaa atatcatcat aaataaacga atgtgcttta
    11581 tcaagccaat agtttaaaat aggggcattt aaagcagggc agtattcatt ttgtctgtaa
    11641 tagtttttag taaattcaac tctttgtttt ttacattgtt ctatagtcaa taagtttcta
    11701 agtttatagt atatttctaa acaatagtta aatatttctt tatggtcaaa ctcatttagt
    11761 aaaactctta aaagttttct tgtaagtcca ttaaatttaa gtttttcaag ttttgtatat
    11821 attttatttt gttttttaga gtcaaggaca tttgaatata tagatgaaaa ccaacttttt
    11881 tctgtttcat tatatccaca tgtacttata ctataaaata agatatcaag tattaatttg
    11941 taatctaagt caagtttttt actagtttct ctccaaagct ctgctaatgg ataattatca
    12001 agagtatcat tagaaacttt atatttaata ggagtcaagt tataagtaga ataacctaaa
    12061 agtactttgt ctacattacc tgaccatctt tcaacttcat attcataatc tgcattttca
    12121 gtaataagtt catcaaattt ttcataaact tttattattt tatttatatc cgtatcgaga
    12181 attttttttg cttctttttt tgttgctaca gaaacagaat cctcacgagt agataaactt
    12241 atatttgaga taattttatt aagctttgat gagctattag aatcatcttt aaaatcaaat
    12301 tgttctggaa tttgaacttt gttttcaggg ttatatattc caaaaccatt ttctttgcag
    12361 taatcatttt ttgctttacc taacaaatta tcaattagta tttgaacttg agatgtattc
    12421 tttttagatt tatcaatatc ctctataagt aaaataagct tattatatag ttcttgtcta
    12481 gcagtattgt cttttaataa gtttacaatt tcaagacctc caaggcattt ttgagaattt
    12541 gaagatttta tcagtgaaga agcaatattt tccaaatttg aatcatcttg ttcaagtaaa
    12601 atttttatta ctgtttgtct aatagagctt gtctttaaag acaatagatt ggttatctta
    12661 tctaattcta catctgttag ctcaagcttt ctaagtagac tcaataccgt ttctctattg
    12721 gaaacactct tatcacttaa acattccaat aaaaattcac gttgtctatc tgtctttggt
    12781 tcatttacaa aattgactat aagtgaagct cttaaatctg aactcataag atgacaattt
    12841 tccataagca catctataaa ttgagcattc atatcgtatg ctgttaaagt catcatacat
    12901 ttaagtattt catgagaaga aatttcaatc caagtccatg gaaatacact tcctgaaaat
    12961 gattttgtct ttgaatttaa aatttctaaa gtatgtttta aactttcaaa ttgttctttt
    13021 ctagcatcaa agtctttgtc aataggagtg tttgacaagt caaaattagt ttctctgttt
    13081 tgccaactat agtagtagcc aacagaacct tctacataga aatttgtaat tacccatgct
    13141 agtatctcta aatcatcacc cttaatgttt ttagatgcta tagaatgttt aaaatatgta
    13201 ctttctgtat gcgataaaaa gtaaagagct acaagttttt tatatttttc ctgacaagaa
    13261 agtaagtttt caataagaga gtaagagtca tatatgttgt aagttgcagt agcccataag
    13321 gcaaaataaa tttcaagatt atcattgcta ttaattgatt ctatacggaa cttttcatca
    13381 gtcaatgatt tatatgaaag ttctaaacat ttacgtatta ttccaggttt ttggtctgaa
    13441 aaaccaagtc ctgtccatgt atccaaggct cttataacag aactaaatcg ctctaaattg
    13501 ttgtccaaaa tagtttttaa catgtatatt gatgcagaaa ttgtaccctc atctaaactc
    13561 tctacaattt gctgtctaag tccttcttgt tgtttggctg ccaaaagaag tttagaaagc
    13621 atttcatagc atttatcact atgactcttc atgataccaa ttatcatttg tcttgtaagt
    13681 agagcagttt gattttctcc aaatattact tcttctaaag cacttattac tttttgattt
    13741 cctttgtcta tttctagtgc tattttatca ctcattatag agtcatagta caagacctta
    13801 tttggaagag taagataatc tataactgaa aaatcataat aaattaaatc aacatagttt
    13861 gaaataaacc ttataatacc tctaaaataa tccacaagtt tatttgagcg atacccttta
    13921 cgataatatc ctcctgaata tgtataatcc atctttcttt taattaattg gcaaagttct
    13981 tctacttgtt cagaagtaaa tattaattct aatgcatttt ttaaaggtcc agaaaggagc
    14041 tcgtccatat ttttaatttc gtagtagtgg caattgtagt atttctttat catatttaag
    14101 aatttgtctt caggttctct ataatagtat gaactatttt ctatgcagtc ccaaagagac
    14161 tttgctaagt cgtagtactg ttttgaagaa gatttagtaa aattattttt aaaattttca
    14221 attattttag gatttttttc tactctagta cgttctaaca tttatgtacc tccaaattat
    14281 ttattttaaa acattcttcc agcaagcaag gtaagtctta taaaagtaaa tatatctcca
    14341 cttttcagat ttatagagga atcgagtttt tcaataacat cctcatttat aagcactttg
    14401 tagattccat cttcaaatgc ctgtaacgct acctctattg ccttttctac agattgttta
    14461 ttctcattgt aaatagagcc aaatccaact tttccaatat cagcttgaga ttcaatttct
    14521 tcattagtta agaagtttat tattgatttt tctgggattt tctcattata ttcaagaaca
    14581 tttttagata caatttcaga gatagcttct tttaggcaat tgatgttgtc agatacatca
    14641 aaaggggtaa gttctagcat aggtttgcgt ttgccaacag ccttaactct aaaataaatt
    14701 tgcataaaat cacctcgcaa aatattaatt atgtatttag aaatttaaaa atttatctat
    14761 ttgttatatt atatcatttt gtgaattatt aatgtgaatt atggaattat tttacaaaaa
    14821 atagaaaaaa agctatctta aagtctaaaa aagcaataaa agataacttt taactataat
    14881 tatatagaat tataactgta catttattaa attttaattt tagaattatt tttgataact
    14941 atcttttttt atttataaat tttgctccta aatatttttt gatatcttct tcaggagaaa
    15001 agtcatcaat aagtatttct ttcccagtgt cttcaaatac tacaagaact tcacagtcac
    15061 cttgtaagaa aaattttcta actgtatctt ctttaacttc tctaactttt attacattct
    15121 ttttttcttt ttcaaagtct tttgtgtaat aacttcccat catattaatt atttttgtat
    15181 atctattcat aaaatacctc catatgcgat taaatttaaa aaatatgtta taataattgt
    15241 ttgtattaac ttaataatta taacaaggag taataataat gactagtata aaaaataaag
    15301 atattttaaa ttgtatagat tattccatta aaaataagct ctttgacaaa ctaaatgatg
    15361 tatataagtc cttgccagta ggaaattgtt ctggatgtgg aaattgttgt atggagtcag
    15421 taggtataaa tttaattgag ttcttgaata tattttgtta tcttgaagat agagttgatt
    15481 taaaaaaaag ctgtataagt aaaatattag attattattt tgaggagtat acaagaaaga
    15541 acccatgtcc atttaaagat actaataata gatgtttaat atatgaagtt agaccattaa
    15601 attgtaggat gtttggacat tggaaaaaag aggattataa taaaaattta gataatgtta
    15661 ttgagaaaaa taataactat agagagttaa tgaaaaagca atatggattt gaaataaatg
    15721 atgaagtagt aaattacagg atagattatt gtgatagatt tattccaaat aaagattatt
    15781 taagtaaatc taagagatta agcttttttg atgagcttat gatattagac tcaaatattt
    15841 attcaagtgg aaatatagat atagacttta gggatagagg tatagttgaa tattttatag
    15901 agtcattact ttatagaaat ttagcatata atgtgaaaat aaggatatct aaagaataca
    15961 aaataagaaa aagaactata gatagaataa aaaggattgt tcttgtatag ttttatatta
    16021 gtaagtaata aattgtatca taaatattgt caaaatgagg tgaagaaggt gaatcttaca
    16081 aaagtaaaag gaaatacctt ttttataaaa ggtggcacga atacaggaat atatgtattt
    16141 gaagataaca cagcacttat gatagaccca gggttatgtg gttcaagacc taggagagta
    16201 aataaagtat tagatgaaaa taatatacag gttagatata ttataaatac tcatggtcat
    16261 gatgaccact atggagcttg taatcaattt aaagaatatt ataaagatgt gtgtataatg
    16321 tcatctgaat acgataaatt atatatagaa aatccagaat tgttttcaaa gtatattgtt
    16381 gggggaaaga gcaatgtatt tatggataat atcttaaaaa ataaaatgtt ggatgatata
    16441 aaaatagata aatctataga agctggaaaa attgaattaa acaatgaaat gtttgatata
    16501 attgaatttc agggtcatac acctggtagt atagctgttt tgacgaagga taatgtgatt
    16561 tttgttggcg atttattgat aggagatgaa attctatcaa aatacgattt cttgtttcta
    16621 tatgatatac aagaacaaat aaattcatta gaaaaattaa aaaatataga ttttgagtac
    16681 ttagttttag ggcatggcaa gcaagttata tcgaaagaag attcttataa attaatagaa
    16741 aaacatttga atgctttaaa aaaatattta tatcaaataa gagagttgct tgtagaaccg
    16801 aaatctttag aaattttatt aaaaaacatt ataaataaca ataatttaag caataattat
    16861 aaagagtatc atttttttaa atcttcgtta gtatctgtca taagttactt agttgattta
    16921 gaagaaatag ggtatatatt ggaaaatgga gaattgcttt actatacaaa aacaaaataa
    16981 attatgataa aataaagaag gtcataaaaa acaaataaag ggtgagcaat atggaatacg
    17041 aaaaatggaa tgagatacta gcaccttatg aacatgcagt agaggaattg aaaataaaat
    17101 ttaaaaatat aagaaaagaa tatttagcaa aaggtgaata ttctcccata gagtttgtca
    17161 caggtagaac aaaaaaaata tcttccataa tatctaagtt aaaaaggata aatgcaaaag
    17221 atatagaaac tgaaattgat gatattgctg gtataagaat aatgtgccag tttgttgagg
    17281 atatatatgc tatagttgat ttgataaaag taagaaatga tatgactata ataggagaaa
    17341 aagattatat tacaaattac aaagatagtg gttatagaag ctatcatgtt ataataaaat
    17401 accctataaa ctccattgcg ggttcaaagg aaattatatg tgaaatacaa ataagaactc
    17461 ttgcaatgaa tttctgggct acaatagaac attcattaaa atataaatat gaccattaca
    17521 tacctgagac tcttgctgaa aggcttagaa gagcatctga tgctgccttt cttttagacc
    17581 aagaaatgag tgaaataaga gaggacataa tgaaagctca ggctatgtac caaatgaagt
    17641 ctatagctat tagagatact ttaaatagaa tacaagagtt atatgacttg ggtgatacac
    17701 ataaagctat gcagtaccaa agaaaacttg atagaactga aacagataaa aatataactg
    17761 aaattttgga attaaaacaa gaaatagata tattattaga gcaatataag gatactaagg
    17821 tagatgataa agtagttcag taaaaactac ttgtagaaaa tttgctaaat attaatgggg
    17881 ctgcctctta atggaaaata atttctaaaa agagacagcc tatttagtgt aattaagaaa
    17941 ggaatgatat tatgagtttg tatgctatag gtgatttaca tttttcaaca tcagtcaata
    18001 agccaatgaa tatctttgga agtaattggg atggtcatga aaagaaaatt atagataatt
    18061 ggaaagaagt tgttaaggaa gaagatatgg tcttagtact aggagataca tcttggggaa
    18121 ttaatttatc cgaagctaaa aaagatttag atataataag caaacttcca ggtcagaaaa
    18181 tacttataaa gggaaatcat gattactggt ggacaaccgt tacaagttta aataagctat
    18241 atgaagacat gcgttttatt cagacaaatt tttatgaata taaagattat gcaatttgtg
    18301 gaggtagggg ctggatatgt cctaatgatg ttaaatttga tgagactgat gaaaaagttt
    18361 ataaaagaga agaacataga cttagactat cattagaatc tgctcgtaaa agtggtcact
    18421 caaaaataat tgtgattact cattatcctc ctacgaatga taaattggaa gaatctttat
    18481 ttacaaagtt atttgaagaa tataatgttg aaaaggtgat ttatggtcac ttgcatggta
    18541 aagaatcttt taaaatggga ttgaagggga ttcgaaatgg agtagaatat acattagcat
    18601 cttgtgacta tacagaattt aatttaataa aagttcacga ttaaaaaaca taaattggta
    18661 aatactataa gatacatgat taaaaataaa tatttgaggg atttcggttt tgagaaagga
    18721 gacacgactt atgaggtgga tattagtttt tatattttta tatcttattc ctttagtagt
    18781 gatattcaga aattacaaga attttaagag gtcttgtatc tacagtagta tttatgtggt
    18841 attagctaca acaataatga taagtaatat ttatatgagt ggacttaata aaataaaaga
    18901 gtctatgtat tatcacaatt atatttctga tgagacatat gatgaaaagt atgtatcaaa
    18961 ttttaaggaa aaagaaagta ctgatttaaa acaggataat aaaaagaatg aagaaatatc
    19021 aaataataaa aatttaaaca acaaacaaga tagaaatata caaaataaag aaagtaatga
    19081 agaaggaagt aatgatataa aagaaaagaa taattctgat agtaatgttt cagtgaataa
    19141 taaatcagaa aaacaagaag ataatacaaa agaaaataat aaatatacaa gtaaagaaaa
    19201 aaatagtgaa gatataaaaa aaatagatat ggaaagtata ggaattttta agaaagaaat
    19261 ttataatata gaaagagcag ctttagttcc aatgagagaa tgtatacctt acactaagga
    19321 tatagcaaaa aatataacta aattatctag tataaaaaaa gatgttacaa atgccagaaa
    19381 caagtgtaaa gaagttgtaa aagaatatga aaatatgcag attccagaac tttctaagga
    19441 gaagtataca gaaacattga gtaatgctag agatgacgta aagaaagcat atgagcttag
    19501 agaaaaatct atggaaagtg ctttgaaatt aataaatact aaaagcccta aatatatagg
    19561 gaagataaca gaatatcttg atttatcaga taagcagata gaaagtttta aaaatagatt
    19621 gaacgattta aaagaagaaa taaataattc agaaacaaat aatattaatt aataagattt
    19681 aaagatataa gttaaaaggg agattatgat gaataaacag agtataggaa aactaaatat
    19741 gttttttatg gggattactc aaagaaacct tgataatagg gattttttta ttgagttaga
    19801 ggtgattttt aaatctggaa gaaaagagtt taaaggaatt gcaatagagg aagataataa
    19861 atataaattt aattttaaag gaaaatcaga tttatatacg tttaatgagt tgcttaagaa
    19921 tatttctaaa gaggcagaaa attatgatgg attagtattt aaatatgttg agcgaggaac
    19981 tattgttgta attgaaggag acaataagaa ggtaaatgta aaatatttag ataataagga
    20041 agaagtacct aaaatagatg aatttacggc atctcaaata aaaaacagag attattatgt
    20101 aaaagtagga caagcaaatg cacttttaaa agaaattgga gtacttacaa aagatggtaa
    20161 gataaaaaat gataaaataa gaaaatacaa tcagatagac cattttgttg aattgataga
    20221 tagtatttta aaagaaataa aggataaaga ctgtataact atcttggatt gtgcttgtgg
    20281 aaaatcttac ttaagctttg tcttaaattt ttatataaaa gaagtattga agaaaaactg
    20341 ttattttata ggaatagatt attcagatgt agttatagaa gcatcaaaaa atatggctaa
    20401 aaacttgggt tataaaaata tgtcatttat aaaagaggat ttgactaatt atacacctaa
    20461 tagggatgtt gaccttgtaa taagtctaca tgcttgtgat acagctacag atatggcaat
    20521 aggtctagga attagagcaa aatctgaagc tatagtagta gtaccatgtt gtcataagga
    20581 gttattaggg caatatagat atgaagctat ggaaccaata ttaaaacatg gtgtatttaa
    20641 agctagattt gcagatttaa taactgatgg tttaagaaca ttgttattgg agggcaatgg
    20701 atatgatact tctgtagtag aatacatatc tccacttgat acaccaaaaa atttaatgat
    20761 aagagcgata aaaactaaaa cgaataatga caaagcatta aaagaatata aagaactgaa
    20821 atcacaattt ggagtggaac caaccttaga aaaacttata tattagttaa aaatgtaaaa
    20881 tttacgaaaa gcttgcttaa atatgcaagc tttttggtat tatataggta tagtcatatc
    20941 cagagttttg atttataatt aaagctgtgc tataatattt gattaatcaa atcaataaat
    21001 gttacatgga ggtaaaaatg aacaaattga atatatgtat tgatatagat ggaacaataa
    21061 caagccctta tcattttctt ccatacctaa atgatatctt taataaaaat ataactgaag
    21121 cagagtgtat aacacataat tgggaagaat tgtatggttc gggaataaga gatgtgtatg
    21181 cggagtttaa taataagtat atacactcat atgatgaagc aaagattgta gagggtgcta
    21241 cagaggtaat tagcacatta tctaaaggac ataatttatc ttttgtaacg gctagacatg
    21301 agtgtttaac tgatgtaact aaaaattggc tatcgagaca aggattttca gatatagatg
    21361 tttacttatt aggaagtgat tataaagttg aaaaggcaag agaactgagt tgtgacatat
    21421 ttatagaaga taatccttta aactcagtgc aactagctga tgatggtgtt aaagtaatac
    21481 ttctggatac taactataat aaagatgtta aacatcaaaa tatagttaga gttagtaatt
    21541 ggatagatat tgaaaagatt ataaaacaag gtatttaaag atttgtagat tgaaaggggg
    21601 agcatatgat agatttaaaa attgaaaata gaaaaggaaa aatccatgta aagagcagtg
    21661 aaataaaaga cattttagaa ttacgtccag acttcgaata tgtacaagat atatcaaata
    21721 ctataaatca agaaaatata ttggtatttg attgccagtt gacaggaaat tcttttgata
    21781 tggatgattt agatatagaa gaaatattgg aagagttagg tgaagaaata gatgagtctt
    21841 atttcagtgt tttatttgaa gatgtaagag cgtatttaaa agatgctact gatgagatag
    21901 aagcagactt acaggataat tacttatttg ataatttgag atgttatttt gatatataca
    21961 atataaatca agaatttaca gattttaagt ttgtattttt agtttctttt aaagatataa
    22021 aaatttcttc attagcaaac ctagctaaga ttgtttctaa gagacagctt ataggtgcat
    22081 ctaagtttta tagttaaaat gaaatattat tataataaaa gctagtaacc ttgtaaaaat
    22141 a
//
LOCUS       DAELTA010000052        22132 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.052, whole genome shotgun sequence.
ACCESSION   DAELTA010000052 DAELTA010000000
VERSION     DAELTA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.052"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            774..2444
                     /locus_tag="KPG13_001696"
     CDS             774..2444
                     /locus_tag="KPG13_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG0985429.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2566..3300)
                     /gene="pflA"
                     /locus_tag="KPG13_001697"
     CDS             complement(2566..3300)
                     /gene="pflA"
                     /locus_tag="KPG13_001697"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG0985430.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3460..5691)
                     /gene="pflB"
                     /locus_tag="KPG13_001698"
     CDS             complement(3460..5691)
                     /gene="pflB"
                     /locus_tag="KPG13_001698"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG0985431.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5984..7045
                     /locus_tag="KPG13_001699"
     CDS             5984..7045
                     /locus_tag="KPG13_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG0985432.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7136..8749
                     /locus_tag="KPG13_001700"
     CDS             7136..8749
                     /locus_tag="KPG13_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0985433.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9024..11009
                     /locus_tag="KPG13_001701"
     CDS             9024..11009
                     /locus_tag="KPG13_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0985434.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11014..11448
                     /locus_tag="KPG13_001702"
     CDS             11014..11448
                     /locus_tag="KPG13_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG0985435.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11477..12037
                     /gene="srlA"
                     /locus_tag="KPG13_001703"
     CDS             11477..12037
                     /gene="srlA"
                     /locus_tag="KPG13_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG0985436.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12101..12469
                     /locus_tag="KPG13_001704"
     CDS             12101..12469
                     /locus_tag="KPG13_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG0985437.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12494..13060
                     /locus_tag="KPG13_001705"
     CDS             12494..13060
                     /locus_tag="KPG13_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG0985438.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13101..13466
                     /locus_tag="KPG13_001706"
     CDS             13101..13466
                     /locus_tag="KPG13_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG0985439.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13567..14340
                     /gene="srlD"
                     /locus_tag="KPG13_001707"
     CDS             13567..14340
                     /gene="srlD"
                     /locus_tag="KPG13_001707"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG0985440.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14689..16833
                     /locus_tag="KPG13_001708"
     CDS             14689..16833
                     /locus_tag="KPG13_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0985441.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16921..17256
                     /gene="spoIIAA"
                     /locus_tag="KPG13_001709"
     CDS             16921..17256
                     /gene="spoIIAA"
                     /locus_tag="KPG13_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG0985442.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17270..17698
                     /locus_tag="KPG13_001710"
     CDS             17270..17698
                     /locus_tag="KPG13_001710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG0985443.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17721..18485
                     /gene="sigF"
                     /locus_tag="KPG13_001711"
     CDS             17721..18485
                     /gene="sigF"
                     /locus_tag="KPG13_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG0985444.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18765..19202
                     /gene="spoVAC"
                     /locus_tag="KPG13_001712"
     CDS             18765..19202
                     /gene="spoVAC"
                     /locus_tag="KPG13_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG0985445.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19369..20382
                     /gene="spoVAD"
                     /locus_tag="KPG13_001713"
     CDS             19369..20382
                     /gene="spoVAD"
                     /locus_tag="KPG13_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG0985446.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20396..20758
                     /gene="spoVAE"
                     /locus_tag="KPG13_001714"
     CDS             20396..20758
                     /gene="spoVAE"
                     /locus_tag="KPG13_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG0985447.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20941..21957
                     /locus_tag="KPG13_001715"
     CDS             20941..21957
                     /locus_tag="KPG13_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0985448.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8626 a         2484 c         3841 g         7181 t
ORIGIN      
        1 tttgtagtgt aatcgtattt tttattaatt cttacaaaat caatgaaaat aagtatatcc
       61 attttaaagt acataaattt aaaaatccag aaaaatatac ttaaattatg taagtaaaag
      121 aggggttgaa tcagttgaaa atgtaatatt agtaaatagt acatcaatcc tatataccct
      181 gtaagcaact ctattttcat gtactaaaaa tgtacctatt ttattaactg aaaagaataa
      241 attaaatgta agtacataag aagatagaaa aaattaaaaa caaaaaatat ttatataaat
      301 aaatataaaa aatgatataa tccctattaa aaaatttata gatgatgaag aaatatcaaa
      361 atcttatatt ataggaagag acacttcttt gccaaaatct atagaaaagc aataccaaat
      421 ccaaagagaa taggcgaaga aaaaaataaa ataaattcaa gagtaataga aaagttttat
      481 agaataaaga actgataact ctttatataa cgaaaaatag aattttaaga gaaaatgatt
      541 taataaatga tttaacatgg tgttttagca ggtaaaaata aatcaccagt aatgattata
      601 gaaaaattac tttatgaatc tcaaaaaact tagttaagag taagagattt gataagataa
      661 ctaaagttgg ggaaatgtgt ttgatgagac aaaaaattag taaaatagta taatctaaat
      721 aaggatattt tgttgattaa actacactaa tagtaatatt gtagggggaa aagatgagag
      781 aagaatcgaa ctctttaagt ttagaccata tagaaatttt ctttgaactt ttatctcaaa
      841 gtacagaaga ttatatattt ttttgggata taaataggaa taaatttaaa atttcttcag
      901 caatttttga tgaatttaac ttaagtaaag agatagaatc tgatttggtc aattgttgga
      961 gtaaaattgt ttatccggat gatgttcaaa tatggaaaga tgatatacaa gaattactac
     1021 atggaaaaaa aggtgagcac aatttagaat ataggttgat taataaatat aaagaaatag
     1081 tatggatttc ttgtagaggt aaagtttatg tgtcggatga cccaaaaact atatttctag
     1141 tcgggagaat aaagaatatt ggtgaaaaaa ataaatttga ttctatcaca ggaacgtgga
     1201 atagagaaca atttgaacat agaatgaatt atcttataaa agagaaaatt tataaaaatg
     1261 gtgctatgtt tattatggat attgataatt ttaaaaatat aaatgaaaaa tatggacatt
     1321 cttatggtga caaagtattg cgtgcaattg ctactgaagt attagaatat cttccaaaag
     1381 atgtaagatt gtataggtta gatggagatg aatttgcatt tttttaccct atgtgtacta
     1441 aagaaactat agagaaaata tatgaaaaga tacagatgta tacaaatact cagcatgaaa
     1501 ttgaatcaaa taagtactat tgcacagtga ctgcaggcgt tgctatgtat ccagaagatg
     1561 gagataatta tttagattta tttaaacatg ctgatattgc attggatata gcaaaaatta
     1621 gtggtaaaaa tagaattaag tttttttcac aagagctcta tgaaaataaa ctaaaggtaa
     1681 tttcaatgca acaaaaacta agagagtgcg ttgaaaataa ttttaatgat tttgagttgt
     1741 tttttcaacc ccaggtaaat gctgttacaa aagaggtaat aggggcagag gtacttttaa
     1801 gatggcattc gagtacttat ggagaagtat ctcctgttga atttatacca atactagaac
     1861 agagtaattt aattatacca gttgggaagt ggattataaa agaagctgta aaacagtgta
     1921 aagaatggca taaaataaat ccagacttta aaatatctgt aaatgtatcg tatattcagt
     1981 taaaggaaga tttttttaga gattttatag tagaatgttt agtagagtat caattaagac
     2041 ctgaattttt aattttggaa ttaacagaga attgttggat accagatata aatttactaa
     2101 atgataaatt tattagttta aagggtatag gagtatatat tgcaatagat gactttggta
     2161 caggatattc atctttaaat taccttaaag aactgtctgt taatataata aaaattgaaa
     2221 gaagttttgt taagaatatt acatataata gttatgaata tacttttctt gaatatatta
     2281 ttaaattagc tcatattata aatttaaaag tttgtgttga gggaatagag tcttatgaag
     2341 agtatgatat agtaaaaagc ttaggtgtag atattattca gggtttttta tttggaagac
     2401 ctgtaagtgc ctctgaattt tataaatttc agctatcaaa gtgatgtata taaaatgaga
     2461 tatatagtat ataaaaatag ggcaaagtgg aagttttgaa gtttattaaa aatttacact
     2521 ttaccctatt ttgttatgtc ttctataaag gattaatata ctacactaac tattatatac
     2581 tttcactcca aatttagaga ttatttcact agctttttgg acatcttcct tagtggcatc
     2641 aggaacatct tttagttcat aatcgatacc taaactttcc catttgtgta ttcccataga
     2701 atggtatggt agcaattcaa atctttctac atttttcaat gtagctacaa attgacctag
     2761 tttttctaaa ttatctatat catcagtaat gccaggaact aaaacatgtc ttatccatac
     2821 aggaatattt cttgattcta agtgtctagc aagttttaat gccttatcca taccaacacc
     2881 agtcaaatca atagattttt cctcaaccat atgttttaaa tcaagtaaaa ccaaatcaat
     2941 attatctaaa actgggtcta tagtttctat atctacaaat ccagatgtat ccaagcaagt
     3001 gtgtatatca ttttcttttg cttttttaaa taattcactt aaaaattctg gttgaagagt
     3061 ggattcacca ccagaggcag ttatacctcc accagaaaac ttcataaaag aagtatactt
     3121 aagagcatcc gttattattt catcaactgt gtattctttt cctgatttaa tatcccatgt
     3181 atccctgtta tgacagtatt tgcatctaag aggacacccc tgaaaaaata atatatatct
     3241 tattcctggt ccatctacag taccaaacgt ttctatagaa tgtatctttc ccttaatcat
     3301 agagttcacc tcacaatata ttttaataac cttatcaata gttataatat ttctctaaaa
     3361 aaggccatct tataccagaa ataattcaaa tataagatag ccttttattt ttatttttta
     3421 cacagtagta atcttagtta aagtataaaa atttaaagac taagccattt taccgtggaa
     3481 tgttctgttt ataacatcta attgttgctc tttagttaat tttatgaagt taactgcata
     3541 tccagatact cttatagtta attgtggata ttcttctgga tgttccatag catcttctaa
     3601 agtagctcta tcaaatacat ttacatttaa gtgatgagca ttttgagcga agtatccatc
     3661 catcattgca gaaagattgt ttattctttc agtcatatct tttcctaaag caccaggtac
     3721 tatagagaaa gtattagata taccatcttg agcatgttca tatggtaatt tagcaactga
     3781 agataatgaa gctaaagcac cattagtatc tctaccatgc attgggttag caccaggagc
     3841 aaatggagca ccagctcttc taccatctgg tgtattacca gttttcttac cataaacaac
     3901 atttgaagtt attgtaagta tagattgagt tggataagaa tttctataag ttttattctt
     3961 tcttatttta ttcataaagc tttcaactaa ctctacagct atactatcaa ctctatcatc
     4021 attatttccg tattttggat aatctccttc tatttcaaaa tcaacagcta ttccttgttc
     4081 atttctaata gttttaacct tagcatgttt tatagcagat aatgagtctg cacaaactga
     4141 taacccagcc ataccacaag ccatagttct aaatatatct ctatcatgta atgccatttc
     4201 taatgcttca taagaatatt tatcatgcat ataatgtatt acatttaaag tatttacata
     4261 taatgttgct aaccaatcag tgaatggttc aaatttactc ataacttcat cataatctaa
     4321 gtactcagaa gttataggtt caaatcttgg tccaacttga acaccagatt tctcatctac
     4381 accaccattt atagcgtata ataaagtttt agctaagtta actctagctc caaagaactg
     4441 catttgttta ccaattctca ttgcagatac acaacaagct ataccatagt catctcccca
     4501 gtatgctctc attaaatcat cattttcata ctgtactgaa cttgtatcta tagatacttt
     4561 agaacagaaa tctttgaatc cttgaggtaa tttagttgac catagaactg ttaagtttgg
     4621 ttctggagaa ggtcctaatg tatatagagt atttaacatt ctaaatgagt ttttagtaac
     4681 taatgttcta ccatctattc ccatacctcc tatacactca gttacccaag ttgggtctcc
     4741 agagaatagg ttattgtaat caggagttct taggaactta accattctta atttcataac
     4801 aaaatggtcc atatactctt gtaattcttc ttctgttacg attccagctt ttaaatctct
     4861 ttcaaagtat atatctaaga aagtagatgt tctacctaaa gacattgcag caccattttg
     4921 gtctttaaca gcacctaaat aaccaaagta taaccattgt acagcttctt ttgagttagt
     4981 tgctggtttt gatatatcaa atccataaga ttcagccatt tttttaagtt catttaaagc
     5041 tgatatttgc tcagttattt cttctcttaa tctgataact tcctcatcca tgcaagaaac
     5101 ttctaatgat ttcttttgct catttttatc ttctattaaa gcatcaacac cgtatagagc
     5161 aactcttctg tagtcaccaa ttattctacc tctaccataa gcatctggaa gacctgttat
     5221 tataccagat tttctagcag ctctcatttc tggagtataa acatcaaaaa ctccttggtt
     5281 gtgtgttttt ctgtatttag taaatatatc ttttatttct gggtctaatt catatccaaa
     5341 agcttcacat gaattttcaa ccatctttat accaccatat ggcatgacag ctctttttaa
     5401 aggggcatcc gtttgtaacc caactattgt ttctaattct ttatctaaat atcctggagc
     5461 atatgcagct attccagaaa cagtttttgt atcaacatct aaagtaccac cattttctct
     5521 ttctttttta aataggacca ttgcttcgtc ccataatttt ttagtatttt cagtggcacc
     5581 agctaagaaa gagtcatttc cctcatatgg agaatagttt aattgtataa actctctaac
     5641 atttatttct ttagtccatc ttcctgtttt aaatccttgc catgcattca ttaagtattt
     5701 cctccttaga tttaaatgat aaataatact attaaaatta atttttatat acttaattta
     5761 ttttatatat ttttttaaaa atatattttc taatataata atagcatatt attcgtatcc
     5821 tgtatacata aaaaataaaa aattgtcgct ttaaagtata aaacagatta tatgttaata
     5881 atgtggtgaa attataccca tatataaata aaataaaaca aataaatagc taattaagga
     5941 gaaaatattt tttatttttt gtttaggata aataaatttt attatggaaa aaagaaaaag
     6001 catacatctt cacaaactga atatttattt aataggggga ggtgatagat tgtttatcac
     6061 agtggtatta tttttagtag gtttcctatt aattactaaa ggcgcagata tctttataaa
     6121 ttgtactgta gagataggta aaaaaacgaa tatatcagaa attatacttg gagctactat
     6181 agttagtttt gcaacaacat tacctgagtt tacagtgtct ctacttgctt ctattgatgg
     6241 tcatactact atgagtttag gaaatgcagt tggttctata atatgtaata cagggttagc
     6301 attagggttg gttgttttta taagtccatt taatgtagat aaaaaaatgt ttttttctaa
     6361 gtctttacta ttaatagtat ccgttatagt tttaatttta ttgagtttag atggagttat
     6421 aacaagaggt gattcattat tacttataat aatattaatc ttttatatgg ttaataatta
     6481 tagaagtgtc gttggaaaat ctgatactaa aaatagaaat attaaaaata acactattaa
     6541 agacatatct actaaaaaag ggaaaaatta tagaggtttt tccgtattag aaattgtaaa
     6601 aatactatta ctttttgcta caggattgat aatgatgata ataggttcac aaatccttat
     6661 agagagtgga gtaataatag ctagtttttt aaatatacct caaggtatag taagtttaac
     6721 tataatagca cttggaactt ctctgccaga gatagtttcg tcgattactg ctataagaaa
     6781 aaatcatcat gaaatatcag taggaaatat tttgggtgca aacatattga atatagtatc
     6841 agtaatagct gtatcagcaa taccaaataa cataccaata ctatctcaaa atagacaatt
     6901 ggatatacca ttcatgatat tattattatt aatagtaata ataccaacac ttaaatctaa
     6961 taaacttagt agaatacaag gtatattgat gttattcaca tattttctgt acatatcaat
     7021 tttgtatttc atgtatatta tttaactata ctgtttaaaa tattaattgc agaatatcct
     7081 aaaaaagtat ataatggata ggttaagatt aattgataac ggagaggaat aatatatgaa
     7141 tataacaaag tttgaagatt taccaataag tgagggaata aaaaaagcta tagctgaaat
     7201 ggggtttgaa gaaccatctc ctatacaagc acagtctata cctgcgattt tgtcaggtaa
     7261 agacgttata ggacaagctc aaactggtac gggaaaaaca gctgctttta gtatccctat
     7321 actagaaaca atagacccaa ataatagaag tttacaagct gtagtgcttt gtccaacaag
     7381 agaactggca atacaagtat caacagaaat aagaaagtta gctaagtatt cgcacggaat
     7441 aaagacatta cctatatatg gtggtcagcc aatagataga caaataaaat ctctaaagag
     7501 tggtgtgcaa gttgtaatag gtacaccagg acgtacaata gaccatatca atcgtaaaac
     7561 attaaagatg gataatgtaa aaatgattat attagatgaa gcagatgaaa tgctagatat
     7621 gggatttaga gaagatatag aaatgatttt aagtaagatt ccagaagaaa gacaaacaac
     7681 tttcttttca gctactatgc caagaggaat tttggaatta actaagagat atcaaaaaga
     7741 cccagagcat ataaaagttg ttagaaaaga acttacagtt tcaaatacaa agcaatacta
     7801 tatagaaact aggtcatcaa ataaattaga agtattatgt agattagtag atgtatatga
     7861 ccctaaacta tctgttgttt tctgtaatac taagagaaaa gcagacgaat tggtaggaga
     7921 tttacaagca agaggatatt ttgcagatgc attacatggt gacttaaaac agactcaaag
     7981 agatatagta atggataagt ttagaaatgg tacaatagat atacttgttg ctactgatgt
     8041 tgcagctaga ggtatagatg ttgatgatgt tgaatgtgta ttcaactatg acttaccaca
     8101 agatgaagaa tattacgttc atagaattgg tagaactggt cgtgctggta gagaaggtat
     8161 gtcatttact tttgtatttg gaaaagaaat gagaaagatg aaagatatag aaagatatac
     8221 taaatctaaa ctaataaagc ataatatacc aacaataact gatgttgaag agaaaaaagt
     8281 aggtactttc tttgcacaag ttaaacaaac tatagaagaa ggtcacttaa ctaaacaatt
     8341 acaatggtta gaaggtttct gtaatgatga agattatgca atggttgata tagctgctgc
     8401 acttgtaaaa ctatctctag gtgaagaaat gaaagaagaa ataatagaag aaaaacctag
     8461 aagagagcgt ggagatagaa aaggtggtac tggagctaaa gatggtatga ttagattatt
     8521 tatcaatatt ggtagaaatc aaagagtaca agcaaaagat atagttggtg ccatagctgg
     8581 agaagttgga ataccaggta aagtagtagg tactatagat atatatgata agtatacttt
     8641 tgttgaaata cctaaaaaag atgcaaaaac agtaattgaa aagatgaaag atattaagat
     8701 aaaaggtaat aagattaata tagaaaaagc aaataagaag aaaaaataac gtatagtata
     8761 caaaactata attagaaaat atataaaata atatattgga tatttaaaaa gcctgatgtg
     8821 ttttcaaaac agatagtaga taatctgtaa gagttaacag cataaggctt ttcttgtttt
     8881 atatgatgtt ataattttcc agattgttgt gaaaaatgta tattagttcc tttttttcaa
     8941 atctgatttg aaaaagagaa tattaattat ctatatagtt tagattataa taaaaaatgt
     9001 aagataacaa aggggagaaa aagatgtcaa tgacttatat ggaccctagg gtttttttga
     9061 ttttagaaat ggtgttagaa gaagacacaa atactctaga acaattttca aatgttttgg
     9121 gtgttagtac taggactatc agaaactata taaaccagat taatcgagaa ttaaaaggtg
     9181 gtattgctaa aatagttaaa aatgaaaaag gtacatatag tataagaatt gaggatgaaa
     9241 gtaaattaat tgagattgta aattttaata gaggaaagct atcaaatttt ataaatttaa
     9301 attcacctga tgaaagaata aattatgttc ttgatatttt aactatgacc aacaatccaa
     9361 ttaccataga tgatttggct gaagagatat gtataggaag aacaacatta ataaaggatt
     9421 taaaaaaagc agacagaatt ttagctgaat atgatttaga attaaaaagg aagccaaata
     9481 taggtatgat tttagttggt gaagaaatgg atataagatt attaatcctt gatagattgt
     9541 atgaaaatta tattgatgtc ttggaaaacg ttaatcagat aaatttagtt aaagattatg
     9601 atattgaatg tcttagagat gaattaaaaa agttatttaa aaaagaagaa ctctacataa
     9661 cagaacaggt cttgagagat gttgaaagat atataatcat gtctgtctta agaaatctaa
     9721 atgggtacaa atttgaaaag atagataaga gatttgaagt gataagatgt tcagatgaat
     9781 atactttagg attaaagctc aaagctttat tggaaaagat atttaatata gttttaaatg
     9841 aaaaagaaac catatttttg acaatgccat taataggtag aaaagctcct agtacaaaat
     9901 tagcacttag cagtataaag ataaatgata gcgtaaagga agttgtagat gaagtaacta
     9961 gttttttatt agagacctca gggattgatt ttaaagaaga taaaaagctt atagaaaatt
    10021 tggaatatca cttgtatttt gcacttaata gaatgagatt caacataagg gtaaaaaatc
    10081 ctcttttaca agaaataaag gaaagatata catttccata tagtgttgca aagatagctg
    10141 caatgataat tgaggaaaag tttgacttga aaatatgtga ccacgaaata ggatatatag
    10201 cattacattt tggaagttat tttgaaaaaa attcaagaaa agttctaaat gtaaataggg
    10261 tagctgttgt ttgtggaaca ggtcttggga ctgcacaact tattagaata aagataggaa
    10321 aaatacttgg agaaagtgta gaagtaaata ctttttctga tatagaagct aaagctaatt
    10381 tagatttatt aaagtattat gatttagtat ttacaacagt agatttcaaa acagacttag
    10441 atgttccaat ttttagagta aatgcgttat ttaatgaaga aagtataaag aaagagatag
    10501 aaaatgcaat acttttgaaa aatagtataa atcttaagtc tataaaaaag attgaaaaaa
    10561 atatcccttt tataggtaaa ttaattgaag aggataagtt ctttatattg aataagagta
    10621 cgtttatgga aaatttagaa gatatgttag aaaaattagt tcataaagga attattgata
    10681 tagagttcaa aaaaaaggta ttaaaaagag aaaagaaatc ttcaacagca ttagataatt
    10741 atatagcatt gcctcactca gttaatacta atggggaaaa tctttatctg gcatttggaa
    10801 tattagaaaa accagtgatt tgggatacaa aggaaattag aataataata ttgatgataa
    10861 ttccagataa gaatgtagat agtacagacc tgataataaa aagttatgaa gaagtacttg
    10921 aagtagggag agataaaaag atggtagaag aacttgctaa ggcaaaatca tttgatgaat
    10981 tttataaaat aattaccaag aggaggtaat aatatggacc cagtaatttt tattatagca
    11041 tcacttatta cagtgtattt attaaatatt tttcttggat acttacagct taaagatttt
    11101 aataaaaatt atattgaact aaaaagaaaa ggaagagtgg caattggcag aaaaaaagga
    11161 agaatttcat cgggtacaat tgtgttgata cttataaatg atgatggtgt gattgtcgag
    11221 actagaaaaa tgcaaggtgt tacagtattg gcaagagtaa agtcatttga aggtttggta
    11281 ggaaagaact tagagtatat agaaaaatca gatttaactg tttacaataa actacttaaa
    11341 aaagcaatac tagatgcagt caaacagtac ataacatttg agaaaaataa atgtgaagat
    11401 acaaataatc tgagtactga tgaagagtgt aggatggaag tagtttaaaa aaatatattt
    11461 ataaggagga gttaatatga cgcaattttt tcaagtatta gcaagtggtg cagatagttt
    11521 tatgaattta tttagggcag gtgggcaaca atttgtaggt tttgtaacag atatagtacc
    11581 acttttagta gcattattag ttacaatgaa tgcaattatc aactttattg gtacagatag
    11641 agtcgagaga cttgcaaaaa aatgttctgc aaatatattt actagatatt tgatattacc
    11701 agtccttggt acatttgtat ttgcaaaccc aatgacacta tcattaggaa gattcttgcc
    11761 agaaaagttt aaacctagtt actttgcagc agcaagcttt tcatgccata caatgaatgg
    11821 attattccca catatcaatc caggtgaact atttatatat ctaggaattg caaatggtat
    11881 aacaactttg ggattctcaa cagcagattt agcagttaga tatttgttag tgggtatagt
    11941 tgcaaacttt attaaaggtg tagttactga ctatacaaca aaatttgtag agaaacaaca
    12001 aggaatagca ttaaattcag atataaagat tggataatta ctttaaggta taaataaatt
    12061 tttcaaataa aaagatagtg tatagaggag gataaattat atggcattta acaaagttaa
    12121 aatagtaaaa ggttctggag gatggggtgg accattgata atagaaccaa cagaaaagaa
    12181 gaataaggta gtatatgtga ctgggggagc aaagccagaa actgcagtaa aaatagctga
    12241 acttacagga tgtgagttaa tagatggatt ttcacagggt gttaaagatg atgaaatcgc
    12301 atgtgtaata ataaattgtg gtggaacatt gagatgtggt atatatcctc aaaagaaaat
    12361 accgactata aatataatga aaacaggaaa aagtggacca ttagcaatgt ttataaaaga
    12421 agatatatat gtttcagggg taaaaccaac aaatgtagag cagttataat tgaggtagta
    12481 ggagggaaga aatatggctt atgatactaa aaagaagata agtgagcaga gtcaaggaat
    12541 tgttgcaaaa ataggtatag cactaggaaa tgtgacaagt actctttttc aagctggtag
    12601 ggatactata gatacattaa tacatactat actgcctttt atggctttcg ttgcagtcct
    12661 aataggtata ataaatcaat caggtatagg aaattgggta gccaatttac ttactccatt
    12721 aatcggaaat gttggaggtt tagttgtctt atcaataata tgttcatttc cgttgttgtc
    12781 tccgtttctt ggaccaggag ctgttatagc tcagatagtg ggagtattgg ttggtacaga
    12841 aataggaaaa ggaaatattc caccaagttt agcattacca gcattgtttg caataaactc
    12901 acaagcagct tgtgacttta taccagtagg tcttggtctt gcagaagctg aacctgatac
    12961 agttcaagta ggagttccgt cagttttata ttctagattt ttaacaggtg caccaactgt
    13021 tctattagca tggttagcaa gctttgggtt atataaatag aagaatactt tataaataat
    13081 tagagagagg ttcagttgat atggaattaa tatacagtac tcaaataaat aaaataggag
    13141 ataatgcaaa tgaattttta caacataata tgtttgtaac tttcaaagat aatgctcctc
    13201 aagagttaga aaattactgt tatatacatt ctgaaaataa tttagtaaaa gaaatactta
    13261 cggatgatgt gctatcaata aataatgttg attacaaaat tacttctgtt ggtgagttag
    13321 taaaccaaaa tctttcagaa ttaggtcata taacatttaa gtttacaggt gaaaaagatg
    13381 gagcaattgg aggaacctta tatcttgaag aaaaagaaat agcacctatt ggcgtaggta
    13441 ctatactaaa aataattaga aaataaaatt taaaacgaat ctaatcatat tataagtaaa
    13501 ttatagataa aagtcaattt aactaatcaa gttaactttt aatgtgataa aaaaggggaa
    13561 atgaaaatga gaaaagtggc tttagtcgtt ggaggaggaa gaagtttagg tgaatattta
    13621 tctaaacatt tagctaaagt aggttatgat gttgctattg cagatttaaa tgaagacaac
    13681 gcaaaaaaag tagctgaaga aataaaaact gaatttggtt gtaaatctgt aggatatagt
    13741 tgtaatgcca tagatgaaag acaggttata agtactgtca aagctattga aaatgatttt
    13801 gatagaatag atttattagt gtacaatgca ggagtggctg taagtaaaaa aataactgat
    13861 tttggataca aagattttaa atttattgta gacgtaaatt taaatgggta ctttctattt
    13921 gcaagagaag tatctaaagt catgatagca aaaggtattg agggtagaat aatacagata
    13981 aattcaaaat caggtagagt gggaagcaag cataattcag gatattcagc aggtaaattt
    14041 gctggagtag gacttactca aagtttagcg ctagatttgg cagaacataa gataactgta
    14101 aatgcactga tgctcggtaa tttacttgat tctgatatgt ttgaaagttt aattcctcaa
    14161 tatgcagtaa aattgggaat acctgaatca gaagtcaagc aagtttatat agataaagta
    14221 cctttaaaaa gagggtgtag atttatagat gttgcaaata tgcttacatt ttatgcttca
    14281 aaggaagctg aatactgcac tggtcaatct ataaatatta ctggtggaca agttatgtaa
    14341 ggtttttatg gagtgtctca agatgaactc tttagttcat gaggcacttt tttgtataaa
    14401 attaaatata ttttcagcat ttaaacaatg aaaaagaggt ttatctttat tttgagatag
    14461 cctcattttt gtaattaaaa aacatagtgt ttaagtaaac atataagatt gttagttttt
    14521 taaaagagaa ttttttaata atattatatt tatgaagtat ttttaaactt attttgataa
    14581 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
    14641 tatatgttaa tttttaataa ggtaagaaag tagaaagaag gatattttat ggatataata
    14701 aaaaaattaa gtcatgggtg tacattagat tgtcatgact gttgtaaatt caatgtttat
    14761 actaaaggaa ataatgtagt aaaaattgaa ggagataaaa atcatcctta tacaaaaggt
    14821 tttatatgca aaaaagggat ggcccattta gatagattaa atcataaaga tagaataaag
    14881 actcctatgc ttaaagttga tggagtatgg aaagagattt cttttgataa agctatagaa
    14941 ataatggcag aaaagcttac atactataaa gaaaagtata catcaaagtc tgtcatgcat
    15001 tatgaccaat atggaagtgg gtctgtgcta aaatacatag gagatatatt ttttaatttt
    15061 tatggaggtg ttagcagaca taaaggtggt ccctgttgga gtgcagggat gcatgctcaa
    15121 aaatatgatt ttggagtagc taagagtcat gctatagaag atatgttaaa tagtaaaagt
    15181 atatttgtgt ggggaaaaaa tccagcatat actacaattc atactatgca aataattaaa
    15241 aaagctaaag aaaaagggat aaagatagta gtgattgacc ccatatatac aaaaacagct
    15301 caaatagcag ataagtatgt acaagtaaat cctggaacag atggagcttt agctatagct
    15361 atggcaaaaa ttatagtaga agataaatta tatgatgaag aatatataaa ctcgtatgtt
    15421 attggttttg aagaatataa aaaatattta tcttcattgg aattaagttt tttaattgat
    15481 gaatgtggtg ttaaagagaa tgatataaga gagttagtgg atttatatac taacaaatat
    15541 tcaagtataa atgttggata tgggcttcaa aaatataaaa atggtggaaa cacaataaga
    15601 gcaatagatg ctttaggtgc tataactggt caaataggat ttagtggtgg aggggtaaac
    15661 tatgctaata aagtttatcc gagtgttata aattctgacc cttacaatag ccaatcatat
    15721 ggtgaagata gggagtttta tgtaagtaat atcagtaaat ttatagaaga atctttaaaa
    15781 aatacatcaa ataaagtaaa ttatgcatca gatgaacttg atatgacatc aaataaagta
    15841 aattatgtat cagatgaact tgatataaca tcaaataaaa cagattatat atcaaatgaa
    15901 ttgtataatt tatctaataa gtcaatcaaa ggtaatattc ctataaagat ggctgtaata
    15961 actaaaagta atatgctgaa tcaactgcca gatttagtag aattggagag ggtgttttca
    16021 aaaattgaat ttaaagtatg ttttgatatg tttataactg atacagctac tttatgtgac
    16081 atgtttatac cgtgtacaaa tacactagag agtgaagata taatttatag ctcaatgacc
    16141 aatccatata taacctataa tgaaagagct gtaaagccag cacataaatt gatggatgaa
    16201 tattacttct ttatggagct agctaaaaag atgggattaa atgattatcc ttttgtagaa
    16261 aagagaactt atttagaaaa agtgattgaa ccacttaagc gttttgacaa aaatctggat
    16321 atagaaaaac ttaaaaacaa ttactttact atacataacc ctgttgcttg ggaagataag
    16381 aagtttgaga ctccatctgg aaaatacgag ctttattcag aaagtataaa aaacttggga
    16441 ataagtccaa cacctgtata tataagcaat aaatataaag aaatagaaga taaaaatatt
    16501 tcttttagat tattaactaa tcatcatgca gatactttat ttagtcagca ttttatggac
    16561 aaaaaatcta tagctcaagc atatataaat cggagaatgg ctaaaaaagt aggtattgag
    16621 gacaaagata ttgtgatatt gaggtcaaaa aaagcaaaaa taaatgtaca aataaatata
    16681 gatgatggag taggtaatta tatagtaaaa atgtatgttg gttggtggaa gaaacatgga
    16741 aatcctaata gtttaacaga cactggtata tcagattttg ggggtcaggt aacatataat
    16801 gaaagtatgg tagaaataat acgacaaaac tagttaaatt tcattgaagg aataaaaata
    16861 taattataga attgattaaa agttactatt tttacaaaac tattgaagga gggatttttg
    16921 atggtaaatt attccttaga acataagaat ctatacatag aatttatgtg ctcagaacta
    16981 gaccaccatg tagccaatga aataagggaa gaaatagata acttattaag tgtaaatcaa
    17041 gtaaaaaatg ttgtatttaa ttttgaaaat attaatttta tggattcttc aggtataggc
    17101 gtcatcatag gtagatataa aaagatttct aatgaagggg gacgagtttc agtaattaat
    17161 ataagttcac gagttaagaa gatttttgat ttatctgggt taaataaaat tattggcatt
    17221 tatgatacgt atgaagaagc tctaagttct ttgtagggag gagtatgtga tgaataatat
    17281 tatggaagtt aaattttcgg caatatctga aaatgaaagt ttagcaagag ttatagtagc
    17341 atcctttgca gctaagttag acccaacagt agatgagtta gtagatataa aaactgctgt
    17401 ctctgaggct gttacgaatg ctataataca tggatatgaa gaagacagtt ctaagtttgt
    17461 tttcttaaga tgtgagatag aaggaaatac tataaagatt atagttgaag atgaagggta
    17521 tggaatagaa aatgtggaga aggcaatgga accactttat acatcaaaac cagaacttga
    17581 tagatcagga atgggattca cagtaatgaa aagctttatg gacgatgtag aggtaagttc
    17641 tgtaaaagat aatgggacta gaattgaaat gactaaaaaa ataaatgtgc ctaaataaag
    17701 tccaaaagtg aggttttatt atggaagtaa ctgttgccag agaagaaaaa aaacctcttc
    17761 ttagtcatga agagactcta gaacttattg aaaaagttca aaatggagat gaagaggcaa
    17821 aagaaatatt aatatcaagc aatttaggtc tagttaggag cgttgtatct agatttttaa
    17881 atataggata tgatagagaa gatttatttc agttaggttc tataggctta ataaaatcaa
    17941 tatataaatt tgacccaaaa tttaatgtaa aattctctac ttatgcagta ccaatgattt
    18001 taggtgaaat aaagagatac ttaagagatg atggtatgat aaaagtatca aggtcgttaa
    18061 agcaaatagc tataaaggca aagatggaaa gtgaagcttt aactaaaaaa ttaggtagag
    18121 agccgagtat agaagaatta gctaaagcaa tagatgtaga aaaagaagat ttagtaatgg
    18181 ctatggaagc taatttcaat gttgagtatt tgcaaggagt tattcatgaa gaggaaggtt
    18241 ctccaatatg ccttattgat aaaataagca tgaaaggaga aagtgaagaa gaaaaagtag
    18301 tggataacat attgttaaaa gacatacttg gaagactgga taagagagag cgtcaaataa
    18361 tagttctcag atactttgaa gacatgactc aaagtgaaat aggagagatg ctcaatatat
    18421 cacaagttca agtatctagg atagagaaga aagtattgtc taagttaaaa gaatatatat
    18481 cgtgataata cttatgatat gtagaataat aaaatataat aaatatatta ctaaaataaa
    18541 atgtaataac tttttatgtt aagtttttaa attattaaaa gatattaaaa taataaaaag
    18601 cattaaaaaa gtaaaaatat aaaaacaagg tgtctttatg gaaaaataga ttttttcttt
    18661 attaggcatc ttttttattt tttgaccaaa ctttagatag tcaaaataaa taataggttt
    18721 atttttttaa aattaacaaa taatacttta gagaggtgtt tagtatggat aaaaattata
    18781 aaaaatatgt agaccaaata agcccaaaac ctacatattt aaaaaactat acacttgcat
    18841 ttattgtagg tggaattatt tgtatgatag ggcaagcaat aaatgatttg tatatgaaag
    18901 ttggaggtct tgataaacta ggtgctagtt ctgcaacatc aataacatta atatttatag
    18961 gggcattttt aactggatta ggagtttacg acctaatagg aaaaagagct ggagctggtt
    19021 ctataattcc aataacaggt tttgctaact caatagtatc tccagcaatg gagtataaaa
    19081 gagaaggcta tgtattaggt gtaggtgcaa acttatttaa aattgcagga ccagttttag
    19141 tttatgggat aggttcttct attttatgtg gaattattta ttatattttt aagatgtttt
    19201 agatgatttt tatttaattt aaattttatt aattatgaag aaggtataaa atctgaaaat
    19261 agctactaaa gtaactaatt gaaaaataat tctgcagatt taattaatat ttttaattaa
    19321 tattaaaaat ttgaatagta aaatgcaaaa ttaaaaaggt gtgaaaatat gaaaaataaa
    19381 agaattggaa aaagaacagt caagttagaa aataaaccaa ccataatatc aactggaact
    19441 atagtaggtc ctaaggaagg tgaaggacca ctaaaggact attttgatat gattatgact
    19501 gacgatttat atggtgaaaa gacttgggaa ttagctgaaa gtaaaatggt agagactgca
    19561 tctcaacaag ctatccaaaa ggcaggtaaa aaattgtcag atgtaaatta tatgctaggt
    19621 ggcgatttaa taaatcaaat agttccagca tcctttgcag caagagaact ggctattcca
    19681 tttttaggta tatatggagc ttgttctaca atggcagaag gtctttgtat aggttctatg
    19741 cttatagatg gtggttttgc tgatttagta ctgtcaggta catcaagtca ctattgtaca
    19801 gcagaaagac aatttagatt cccacttgaa cttggaaatc aaaaaccaat gacagcccag
    19861 tggacagtta caggagcagg aagtgtatta ttagctccta atggagacgg tccaaaagta
    19921 aagtatgtta ctgttggtaa agtaatagat gagggtattg atgatggtaa taatatggga
    19981 ccagctatgg ctccagcagc aatagatact atctactctt actttgaaga tactaatgat
    20041 gacccaaata gctttgatat aattgctact ggagatttag gtactcttgg aaaacagata
    20101 gcagaagact ttctaaaaga aaaaggtgta gatatatcaa aagtttatac tgactgtggt
    20161 atagagatgt ttaaccttaa agaacaagat gtccactgtg gtggaagtgg atgtgggtgt
    20221 tcagctacag tgtttgcagg atatatatat gataagttga gaaaaaaaga atttaacaaa
    20281 gtaatgcttg tatcaacagg agcactactt tcaccgacaa gtacactaca aaagcagaca
    20341 ataccatgtg ttgcacatgc agtagtaata gtaaatgagt aggaagtgat ataatatgtt
    20401 aatggactat gtaagagtat ttattgtagg tggaattata tgtttaatag cccaagtaat
    20461 gatggattat ttcaaaatgc aaactccata tgttatggta acgtatgtaa caactggtgt
    20521 agttttaact tttctaggac tttatgagcc attagttaag tttgcagcag caggtgcaac
    20581 agttcctata ataggatttg gatattcact tgctacagga gtaataaaat caattcaaag
    20641 tgatggtttt ttaggtattt ttttaggagg tacaactgct acagctggtg gaatagcagc
    20701 agctatattc tttggatata ttatgtcagt agtatttact ccaaaagcaa aaccataaat
    20761 ctttattaaa agaataataa gtttttgaaa taataacttt ttataaagca ggtggatatc
    20821 ttttagattc cacctgtttt ttatataaag aagcgtttac ataattgaag ttgacaatta
    20881 tttacaacat agtgtagtat tatattatca aaataaaatg aagtgaagta ggggataaag
    20941 atggtaacta ttaaagatat tgcaaggaat ctaggtatca gttattcaac tgtttctagg
    21001 tgcttaaaca ataatcctaa tgtatctgaa aaaacaaaaa agaaagtggt agaagaggca
    21061 aatagactag ggttccattt taatgtaaat gcaagaaatt tagcaaaaaa agaaactaac
    21121 aggatagggg taatcttttc aaataatttt aatcatcaag atactagaaa gttttttagt
    21181 gatataatgg atagttctat aaattctatt gagactaata aatacgattt tataatacag
    21241 cctaataata acattagtgg tgatagtaat gtatataaaa tggtaaatgg acaaatggtt
    21301 gacggattag taattgtgtc tcaatcaata aaaaaagaag agtatgattt tttaaaagac
    21361 aataattttc cacatgtatt tatatatttt aaaccatctt ttataggaga agtagataat
    21421 ttcttttggg acgataacgt gtatggaggt tatatagcta ccaagcattt aattgaccat
    21481 ggtcataaag atataataac agtaacttca gatgataaga gttcaaaaat gcatgaagat
    21541 agaaccaagg gatatttgaa tgctatggaa gaagcaaatt taaagacaga agtcattaaa
    21601 tctaaaatgg attttgaatc tcaagttgaa tttttagaaa aatacattga taaaataaaa
    21661 aaagcttcag ctatatttgt acaacaagat gtaccagcga tttctataat acaagagtta
    21721 aaaactactt atggtataaa tgtaccagaa gatatttcca taatagggta taataatatt
    21781 gaattaatat catattttag gtcgcattta tcaacaatag atgacccaag agagcaagtt
    21841 ataaaaaatg gagtagatag tttagtaaat ataataaata aaaagagtac agaacacatt
    21901 ccaagaaaat tatatccaag gttaataata cgaaatagcg ttaaaagaat aaagtagtaa
    21961 gcttttaagt atcaaacgtt tattaaaaat attatagatt atgtgaagtt ttttaacaaa
    22021 taatataaat aaaaattaaa aggagtaggt gtatataagt acattgtact cctttattta
    22081 ttgagatagt ttttataata tgaatgaata attttattca attatttgaa aa
//
LOCUS       DAELTA010000053        22131 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.053, whole genome shotgun sequence.
ACCESSION   DAELTA010000053 DAELTA010000000
VERSION     DAELTA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.053"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            444..1514
                     /locus_tag="KPG13_001716"
     CDS             444..1514
                     /locus_tag="KPG13_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985449.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KPG13_001717"
     CDS             1498..2367
                     /locus_tag="KPG13_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG0985450.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KPG13_001718"
     CDS             2411..3271
                     /locus_tag="KPG13_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG0985451.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KPG13_001719"
     CDS             3302..4606
                     /locus_tag="KPG13_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0985452.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KPG13_001720"
     CDS             4669..5931
                     /locus_tag="KPG13_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG0985453.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KPG13_001721"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KPG13_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG0985454.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KPG13_001722"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KPG13_001722"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0985455.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KPG13_001723"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KPG13_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG0985456.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KPG13_001724"
     CDS             13008..13937
                     /locus_tag="KPG13_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG0985457.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14149..15945
                     /locus_tag="KPG13_001725"
     CDS             14149..15945
                     /locus_tag="KPG13_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG0985458.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16085..17866)
                     /locus_tag="KPG13_001726"
     CDS             complement(16085..17866)
                     /locus_tag="KPG13_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG0985459.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17889..19775)
                     /locus_tag="KPG13_001727"
     CDS             complement(17889..19775)
                     /locus_tag="KPG13_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG0985460.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19792..20289)
                     /locus_tag="KPG13_001728"
     CDS             complement(19792..20289)
                     /locus_tag="KPG13_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG0985461.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20607..22088)
                     /gene="cls"
                     /locus_tag="KPG13_001729"
     CDS             complement(20607..22088)
                     /gene="cls"
                     /locus_tag="KPG13_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG0985462.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8510 a         2967 c         3695 g         6959 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aatcaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat tatgttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gagttaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaacat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacatac aacaattcac ggtgtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tggactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatataa tgtttattaa
    14041 taagatatat tatataagga taatttaaag tacagtgtta gattataata atactagatt
    14101 aattttatat ttgagtttca ttatataaca agaaagttgg aattttttat gggaatagaa
    14161 tattttagta aacttttaga aagtagtact gaaaaagaat tgctcttaag taaaaagtca
    14221 aataacataa gtacagcaag attaatatct tttttgatag tgatagcagg gcttgctatt
    14281 ggattttata ataaaaatat agtaggtatt tttgttggag ttttatcaat aataatattt
    14341 atagcactct tagttattca tagtaaagta aaagaagaag aaacatattt taaaagtaag
    14401 agcgaagtat taaataagta tattaagaga tttggagatg gttggaagga atttaaaata
    14461 gatggtaaag aatacttaaa agatgaaaat tcgcaagcaa aagatttaga cttatttgga
    14521 agagcatcat tgtatcaata tatctgtgta gctaatacat cttatgggaa aaatttttta
    14581 gccaaatatt tgtggaatga aaatccagat gagaatataa ttttagaaag acagagagct
    14641 ataaaagaac ttttatcaaa agatgatttt tctattcata ttcaaacact cagtaatata
    14701 attggtaaag agcaaaaaaa caattcagat agtagtatag aatcgtttat agaatatgga
    14761 gaaaataaga atgtgtatat accaaaatgg atgcatattt ttacttgggg tttaccaact
    14821 gcaacaatct tatcttttat attttgtatg ttaggatttt tacctgtttt acctgttttt
    14881 ttacttttta taatccagtt agggtttagt ggttttggat atccaagact tatgcaaact
    14941 cttgctccat tattttcatt tagtagaagt atacaagtat atgaaaaaat gtttgaggtt
    15001 ttagaaaaag agacgtttga gagtgcatat ttgaaagaat tacaagataa actttctaag
    15061 ggaagtggag tttcaagagg tgttaaacaa ttaaattcaa taggaaatgc tgtaaaccta
    15121 aggtataatc agatactata tataattgcc tgtggtgttt tgatgtggaa ctatcattgt
    15181 gcagaagctt tggagcgatg gaaaggtata tatggaaatc aaattagaga ttggtttgaa
    15241 tctattggag aatttgaagc tctaataagt ttaacagttg tttctcatgt aaaggaaaat
    15301 acatgttttc cagtaataaa atatgaagat actccaagat tgaaagttga agaggtatac
    15361 cacccattaa tagcagaaaa aagtgtcata gcaaattcga tacaattaaa ttctcagact
    15421 tgtattatca caggttcaaa tatgtctgga aagacaacat ttttaagaag tatcggtgta
    15481 aacttagtgc tggcatatgc aggtgctcca gtatgcgcta agagttttga tgcgacctgt
    15541 atggctatat ttacttctat gagaatacaa gatgatgtaa gtcaaggaat atcaactttc
    15601 tatgctgaga ttcttagaat aaaatcaatg attcaatata gtttaaaaga actgccaatg
    15661 ttggttctag tagatgaaat ttttaaggga actaactctg ctgatagaat tattggagca
    15721 agtgaggcag taaagaaatt atcaaaaccc tgggttataa gtatggtgac aacacatgac
    15781 tttgagctat gtgacctttc aggtagcgga gatgttgaag ttgtaaacta tcatttctca
    15841 gagtactatg tagacgataa aattcatttt gactataaga taaaagatgg aagatgtaaa
    15901 actacaaatg caaaacaact tatgagaatg gcaggtattc tttaattaat agataggtta
    15961 attaataaat aagtctattt agaaatataa tttaatttgt gaaattaata aggattgtct
    16021 taaattacgt aaaagtattt taagacaatc cttttttaag aataaatttt atactttgtt
    16081 atgactactt agcttcttca caagcacatt cttgtggaca ctctccatag acacaactct
    16141 tatcaaagta atgagtatga aggtatttat gagctacatc actatttggt tctccaaaga
    16201 agtctttata aatagcttgt aaatcaggat tctcatgaga tttacgaata gccttatttc
    16261 tatctatttc atataacgca tttgcacgtc tatcaactat ttcagtatta ccatggtgat
    16321 aaggttgacc agcacctcca acacatccac caggacatgc cataacttct atgatatggt
    16381 atttacattc tccacttctg accttatcca taatctttct agcgttttta agtgtattta
    16441 caatacatac attaactgta gttccatcaa catctatact tgcttcccta attcctttaa
    16501 gacctctaac attagtgaag tttacattat ctaaagtctt attagttatc ttctcatatg
    16561 aagtacgtag ggcagcttct aaaactccac cacttgctcc aaatatagat gcagcacctg
    16621 tagattttcc tagaggatta tcaaaatctt ggtcttgaag attaggtaaa tcgattgcag
    16681 cttcttttat catcttagct aactctctag tagttataga aaggtcaaca tctagtattc
    16741 cagattcact taattcttct cttgaagctt catatttttt agctacacaa ggcattacag
    16801 atacaacata aagttcttca ggattgattc ctaatatttt aggtgcatag tagtttttag
    16861 ctatagaacc aaacatacct tgaggagact tacagctaga tgctaagttt aacttgtctg
    16921 gataattgtg ctctaagaag tttacccatg caggacaaca acttgttaat ataggaagat
    16981 tttcaccttt ttggattctt tctataaatt cagtagcttc ttccattata gtaaaatctg
    17041 caccaaagtt tgtatcaaat acatgttcaa atccaagtgc ttttaaagca gctaccattt
    17101 tccctgtaga gattgaccct ggttctaatc caaactcttc accaatagca actcttacag
    17161 caggagcaac ttgcactact atagtctttt cttttttatt taaaacatcc caaagtttag
    17221 gtacattatc tacttctgtt aatgctccag ttggacagac agatatacat tgaccacaga
    17281 atgtacactc tgtttcaacc atatcagagt taaagaatgt actaactaaa gtattaaatc
    17341 cacgattaac tccagataat gctcctactg tttgtatgtc attacataca gtctcacatc
    17401 ttctacataa tatacactta gaatggtctt taactaaaga tttagtagaa gtatctttac
    17461 ctgcaaaaga tttagaacct tgatatctta tttttcttac tcctaaatca gcagctatag
    17521 tctgtaattc acaatctcca tttttttcac agacaaaaca atcttgtgga tggtctgata
    17581 acaataattc aacaatagtt ctacgagcat ttaatgcttt agcactatta gtctgaactt
    17641 tcataccttc ctttataact gttccacaag ctggaactaa tcctctctct gtctcaacca
    17701 tacaaacacg acaagaagca caagtgtcaa gcttatctat ttcattcata tgtaaatgac
    17761 aaaggttgtg tatttttata tttattaatt tcgcagcatc taagatagaa gttccactag
    17821 gtgctgaaac gtgtttgcca ttaatagtta aattaactaa actcattgtt attcctcctc
    17881 caaataagtt atttcttgat tatagcgtta aatgtacaat tatctataca agcaccacat
    17941 tttagacact tagaagtatc tattgtatgt tgttccttaa cagaacctgt tatacaacca
    18001 gcaggacaca ctctagaaca ttttgtacaa cccttacagt catctgttat aaagtaagaa
    18061 agtaagttct gacatttacc agcaggacat ttcttatcta caacatgagc tatatactca
    18121 tctttgaaat attgaagagt acttaaaaca gggtttggag cagctttacc aagaccacat
    18181 aatgaagcag tttgtatagt ttcagcaagg ttttctaggt catctaaatc ttgtagagta
    18241 ccattacctt ctgtaatttt agttaatatt tctaataatc ttgtagtacc tatacgacaa
    18301 ggtgtacatt taccacaaga ttcttcaaca gtaaactcaa ggaagaactt ggctatatct
    18361 accatacagt cagattcatc aagaacaagc ataccaccag agcccatcat tgaaccgata
    18421 gaacttaatg aaccaaagtc tataggagtg tctaaatgtt cagttggtat acatccacca
    18481 gatggtccac ctgtttgaac agccttaaat ttcttacctt cagggattcc cccaccaatt
    18541 tcaaatacaa tatctcttaa agtagtaccc attggaactt caacaagtcc tacattttct
    18601 acctttccac caagagcaaa gactttagta ccactagaat cttcagtacc taagttggca
    18661 aaccaatctc cacccttaag tataattgca ggtatgttag caaaagtctc aacgttattt
    18721 aagcaagtag gagatttcca tagaccactc ttagaagaac tataagtttt cattctaggt
    18781 tcaccacgtc taccttcaat tgaatgcatt agcgctgtac cttcaccaca aacgaaagca
    18841 cctgcaccat attttaattc taatttaaag ttaaatccag tgcctaagat attttcgcca
    18901 agtaaaccgt atttttcagc ttgagctatg gctactttta atctttctat agattttgga
    18961 tattctgctc ttatgtatat gtatcctgta tcagaaccta tagcgtatcc acatattgcc
    19021 atagcttcta gtacactatg agggtcacct tcaagtacag atctatccat aaatgcacct
    19081 gggtcacctt catcagcatt acatacaaca aactttttat ctgttggacc agctggattt
    19141 tttgaagcag cttcccattt agagccagta gggaaacctg caccacctct accacgaagg
    19201 ccagaagttt ttacctctgc aataacttct tgaggagtaa gagaagttaa acatttgcct
    19261 agagctaaat aaccatcatt ggcaatatat tcttctatac tatctgggtc tataagacca
    19321 caatttttta aagctatacg taactgtttt ttgtagaaag acatttcttc ttgttcttca
    19381 acttttttac ttaaagattc ttcctcatat aataattcct cgacaactgt atttctaatt
    19441 aagtggcttt gaactatctt ttcagcatca gatggctcaa cttttacgta gaaaacgtta
    19501 tcaggataaa cttttacaat aggaccttga gcacagaaac caaaacaacc tgtcaaacga
    19561 acactaactt tatcttggat tcctgatttc tttatttcat tttctaattc actaactatt
    19621 tctagactgt ttgaagatgt acatccagta tcacaacaaa caagtaattc tcttcttaat
    19681 acattttcct tagaataatt ttttcttaag ctaagattag gtttcagctc atcagctaaa
    19741 acttttaatt catcaaatga atttacttta cgcattattt attcacccct cttaacaatt
    19801 caattctaat tctctataag tatctaatat ttcgcttact tggtcaggtt tcactttacc
    19861 ataaacttta ccatttacag taacaactgg agctaaacca catgccccca aacatcttaa
    19921 accttctaaa gaaaatttaa aatcactagt agtttctcca acttttatgc caagctgttt
    19981 ttcaaaagca cttaagattt tgtcagcacc ttttacaaaa catacagttc ctagacagac
    20041 actgatttta tacttaccta caggttctgt agtaaaataa gaatagaatg taacaacccc
    20101 atataccttt gcaggagcta ctccaagttt tctagcaaca tgaagttgaa cttcttttgg
    20161 aagatatcca aatatacctt gggcttcatg tagaacttgt attaacgcac cttcctttgt
    20221 tgcaagagag tcaataaata tatctagttc gtcaaataat tgcttgttgt gtgaaataaa
    20281 atcgcacata acaaatcccc cttttctttt taaagttatt atttttcata aaataattaa
    20341 ccatgagttg accccaacat tatgtgggtg aacaaaacca aaacaataaa gaaagaataa
    20401 tatacaacaa tttctttatt tataaaaaca atgcaaaaaa gttacctatt aaaaataaaa
    20461 actatactag tctattataa tataaaataa ttcagaatag ttaaaaaaaa taaaaaaaat
    20521 gagctaaatt caataaaaaa aaaataatac atataaaaaa ctttaaaaaa taaaccaaaa
    20581 tgtaaaattt ttataaaata aaagatttat aaaactgctg ataatagtct tgaaattgat
    20641 tccttgattt ttatataagt agacctagaa ttatatatat ctaatgttat ttccttagaa
    20701 tttaaaatat cattttcaaa tataattctt tgttccaatg ctttttttga agagtacata
    20761 aatgcattaa cttcgaaatt aagctcaaaa cttctgatat ccatattagc tgttcctata
    20821 gagcatatag agtcatctat aactattgtc ttagcatgta aaaatgcatt ttgaccataa
    20881 gtatagattt tagctccaaa ctttaaaagt tctccagcat atgaatatga tgcccagtat
    20941 acaaagggat ggtcaggttt agaaggaatc atcactctca catcaacacc agataaacag
    21001 gctattttta aagtatctat aaaagttgag tcaagtatta aataaggact ctgtatgtat
    21061 atatactttc tagctttctg aatcatttta aggtaaccat atttaatctc atcaagttct
    21121 gatatatctg gaccacttga tactatctga atcccaatat tttcagaagg atgattagaa
    21181 tttgtatctt cttctataaa gtatttttct aaatctaagt cttctttagt tgtatatctc
    21241 caatcaagga tgaatctcat atttaggtca attacgcaat caccagtaag cttagtatgt
    21301 gtatctctcc atagaccaaa ttttggattt ctacctaaat attcatcacc aacattatat
    21361 ccacctacaa atcctacctt accatcaatg acaacaattt ttcggtgatt tctatagttt
    21421 aagttaaagt ttacaatttt caagaatgat gggaagaagc ttccaacttt aacaccactt
    21481 tctttaagtc tggaaagtgt actatttttt agtgttctac caccaactgc atcaaataaa
    21541 agtcttactt caacaccttc tttagctttg tctataagaa tgtctattat ttcagttccg
    21601 attttgtcat ctttgaaaat gtaaaattgt atgtttatat attttttagc ttttttaagt
    21661 tcttcaagaa gactgttaaa aaattgacta gattcagcat aaatccacac atcgttatta
    21721 ttggtataat gagcattatt tgaattagcc aatgcataaa tcatatcctt atgttgatgt
    21781 atatctgaat caatttcaga tgtagattgg agtttaacct gagtatctag tatattactt
    21841 ttgattatct tatcatcctt ttctttcaat ctaaacatat tgttttttga aatatttctg
    21901 ccaaaagcta tataaagtat gaatccaaga gcaggaatca atactaatat aagaagccat
    21961 gttaaagttg tatttatgtt tcttttttct ctaaagataa ggtttagtat aaccatgaaa
    22021 ttaattatat atattgtagt aactgctatt tcatatattg acatttcgaa aaatgatata
    22081 tcaaacataa aattccctcc ttcaaatatt tattaaaata agtaattata t
//
LOCUS       DAELTA010000054        22131 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.054, whole genome shotgun sequence.
ACCESSION   DAELTA010000054 DAELTA010000000
VERSION     DAELTA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.054"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            51..386
                     /locus_tag="KPG13_001730"
     CDS             51..386
                     /locus_tag="KPG13_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985463.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            523..1800
                     /locus_tag="KPG13_001731"
     CDS             523..1800
                     /locus_tag="KPG13_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG0985464.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1921..3309
                     /locus_tag="KPG13_001732"
     CDS             1921..3309
                     /locus_tag="KPG13_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0985465.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3680..4393
                     /locus_tag="KPG13_001733"
     CDS             3680..4393
                     /locus_tag="KPG13_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG0985466.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKSKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4511..5380
                     /locus_tag="KPG13_001734"
     CDS             4511..5380
                     /locus_tag="KPG13_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0985467.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5535..6680)
                     /locus_tag="KPG13_001735"
     CDS             complement(5535..6680)
                     /locus_tag="KPG13_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG0985468.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7013..7789)
                     /locus_tag="KPG13_001736"
     CDS             complement(7013..7789)
                     /locus_tag="KPG13_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0985469.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8371..9345
                     /locus_tag="KPG13_001737"
     CDS             8371..9345
                     /locus_tag="KPG13_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG0985470.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9443..9748
                     /locus_tag="KPG13_001738"
     CDS             9443..9748
                     /locus_tag="KPG13_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0985471.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9904..11064
                     /locus_tag="KPG13_001739"
     CDS             9904..11064
                     /locus_tag="KPG13_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG0985472.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11114..12016
                     /locus_tag="KPG13_001740"
     CDS             11114..12016
                     /locus_tag="KPG13_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG0985473.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12096..12989
                     /locus_tag="KPG13_001741"
     CDS             12096..12989
                     /locus_tag="KPG13_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG0985474.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13042..13223
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13451..14581
                     /locus_tag="KPG13_001742"
     CDS             13451..14581
                     /locus_tag="KPG13_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0985475.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14653..15678
                     /locus_tag="KPG13_001743"
     CDS             14653..15678
                     /locus_tag="KPG13_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG0985476.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15681..16877
                     /locus_tag="KPG13_001744"
     CDS             15681..16877
                     /locus_tag="KPG13_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985477.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17005..17730)
                     /locus_tag="KPG13_001745"
     CDS             complement(17005..17730)
                     /locus_tag="KPG13_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG0985478.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            18208..20307
                     /gene="nrdE"
                     /locus_tag="KPG13_001746"
     CDS             18208..20307
                     /gene="nrdE"
                     /locus_tag="KPG13_001746"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG0985479.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            20320..21291
                     /gene="nrdF"
                     /locus_tag="KPG13_001747"
     CDS             20320..21291
                     /gene="nrdF"
                     /locus_tag="KPG13_001747"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG0985480.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            21367..21993
                     /locus_tag="KPG13_001748"
     CDS             21367..21993
                     /locus_tag="KPG13_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG0985481.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         8572 a         2449 c         3882 g         7228 t
ORIGIN      
        1 tgctataaaa gtttgtttaa taaattaaat aaagaaaaga ggagaataaa atgtacaaca
       61 tagatgatat gatatataat tgtccagtag aagctttatc aaatatttta ggtaaaaaat
      121 gggtggctat aattatatgg aaaatacaag aagataaaaa acgatttgga gagctacaaa
      181 gagctatacc agactgtagt aagaaaatgt tagttcaaca actagatttt ttaattgaac
      241 aggggattat agtcaaccaa aaaaagttgg ttaataatgt tgtagagtca acatattttt
      301 taagtgaatc aggattacga ttgcttcctg tcatggaaaa aatgattatg tggagcaata
      361 aaaatttaaa ttgtgataaa tactaaaatt tactctgtaa gtaatgaaaa tttaattaag
      421 ctatgagtag aaaaatgtat taaaattaca taatttttta gttagatttt aatttagaaa
      481 tgggattgta atattggaaa taaaataagg aggaatttta atttgcaaaa tgtaaattta
      541 tttttagtat ttatagaagg tatagtatca ttcttttcac catgtatact gccaattttg
      601 cctatttatt tgagtatatt gtcaaatagt agtgtagaaa atttaaaaga aggaaagacg
      661 agttttatag gaagttcttt atttaaaaat actatatttt ttgctcttgg aatctcaaca
      721 acattcttta tattgggttc atcagtaaaa gtattgagta tgttttttaa tgaaaataaa
      781 gatttaataa tgtttattgg tgggattata attattatca tgggattatt ttacatggga
      841 ataattaaat cttcaatatt gaatagagaa aaaagattta atgttaagtt taaagaaatg
      901 aaagcaataa cagcttttat attaggtttt acatttagtt ttggatggac accttgtatt
      961 ggtccaatat tggcatctgt attagtcatg gtttcaagtt caagtaatca tcttagtgcg
     1021 aatcttttaa tagcagtata tactattggt tttattttgc cattcataat tacagctatg
     1081 ttttacagta aactatttaa aactatagat aagataaaat ctaacatgga aattattaaa
     1141 aaaataggtg ggattatact tatagtatca ggaatattaa tgatggtaaa tggatttgga
     1201 agtataagca aacactttaa tacgtcacaa aatagtaaaa ttgaaagcaa gcaagaagaa
     1261 aataagcgag aaaattctac tgataaagaa gaaaattctg atggtaatga tagtcaaaaa
     1321 gactctaata atgacaataa tgataaaggg tctaatgatg aagatagaat aaagtctatt
     1381 gattttactt taacagacca gtatggtaaa acacataaat taagtgatta tgaaggcaaa
     1441 gttgtattct taaatttttg ggctacatgg tgtcctccat gtaaagaaga aatgccttat
     1501 atagaacaac tgtataaaga ctataataaa aataatgatg atgtagtaat tttaggagta
     1561 gcttcaccta atttaggtag agaaggttca agggaacatg tagttaactt cttaaaagac
     1621 caaggttata cattccctgt tgtacttgat gaagatggag cattagcata tcaatatgga
     1681 attaatgctt ttccaactac atttataatt gacaaggaag gatatgtaac tcagtatatt
     1741 ccaggagcta tggataaggc aactatggca tcatttattg aaaatcagag aaataaataa
     1801 aagtatttaa gtataagttt ataaatcaat ttaaggttac aaaaaagtgc ctaattgtac
     1861 cacctaaata atcttgttat agtaaaagta taaacaatac aagaaagggt tgaattttat
     1921 atgaaaaaaa catttgatgc tataataata ggatttggaa aaggtggaaa aacattagca
     1981 ggggatttgg caaacagggg attaaaagta gctttaatag aaaagtcaaa caagatgtat
     2041 ggtggaacat gtgtaaatgt ggcatgtata ccaacaaaaa gcttagaaaa ttcagcaaac
     2101 agcgtaaaaa caaagaatat aaattcttgg gatgaagtac aagctgaata tgagaaagct
     2161 atagataaaa aagaaacttt aattacaaaa ctcagagaag ctaactataa taaattaaat
     2221 tcaaatgaga atgtaactat attcacagga atgggaactt tcattgatga aaaaacagtt
     2281 caagtcaaaa ctgaaaatga aatttatgag ttagtagcag ataatatatt tattaatact
     2341 ggttcaagac catttatacc taatattaag ggaattgaaa ataagaatat agtatatgat
     2401 agtgagtctc ttatgaatct aagaacttta cctaaaaaaa tgacaattat aggagctgga
     2461 tttataggtt tagaatttgc tggtatttat agttcatttg gagcagaagt tacaatatta
     2521 aactctaata atggcatttt accaaatgaa gatgttgagg attcagaaga aataataaaa
     2581 ttacttgcaa agagaaatgt aaaaattgtt aataatgcaa atataaaaga gattaaagaa
     2641 gtttctgaat tagcaatagt agaatatgaa gtagatggta aatctaaaga attaacaagt
     2701 aatatgatac ttgtagctac aggaagaaaa gcaaatactg aggagctagg gctagaaaat
     2761 gcaggtatag aattaaatga aagaggattt attaaagtct cagaaactct taagactaat
     2821 aaagagcata tatgggctat aggagatata aatggaggtc cacaatttac ttatatctca
     2881 ttggatgatt atcgcatagt tattaatcag ttgtttggag ataagactag aacaactaat
     2941 gatagaaaga atatcccaaa ttctatattt ataagtccag cattttcaag agttggtctt
     3001 aatgttaaac aagctaaaga aaagggatat gaagtattag tggcaaagat gccagtagaa
     3061 gcaattccaa gagctaaaca gattggcaag gcagatgggt ttataaaaat agtgatagat
     3121 aaaaaatcca ataagatact tggagctagt atgatatgtg aaaattcaag tgagattatc
     3181 catcttattc aattagctgt agatttagaa gtagagtata catatttaag agaccgtgtt
     3241 tatgcacatc caactatgac agaagctctt aatgatattc tatctcctaa tatgataaaa
     3301 gaagtataac ataagtttaa tatataaacc taaaattatc attataagta gattttagaa
     3361 agaggctatc tcaaaaaata gagataagtc tcttttttac tgtttaaatg ataaaaatat
     3421 atttgatttt ataaaagaag attatcttat gaactaaaaa gtttatttta aaacactacc
     3481 tatttatgtt ataaacttat aaatataata tagaatctat tattctgtat tattaagtgg
     3541 ggttaagcgt tataattaat tataaatatt gaataatcta aatggataag ataatggtat
     3601 taaaaattat cttaaaaggt gaaataatct gtatttaatg tagtacagtt atatctttaa
     3661 agcaaggtgg tgaaatgata taaataagat atctaaaaga gggtttgatg atagtgataa
     3721 aagatggttt tcaagtaaag aattaacaag acttataaag tctaaagaag aaatagaatg
     3781 gttattaaat agggattaca aaatagaccc agtcgttact tttgttggtg atagatatca
     3841 gttttctata agacaaagag atgcattaaa aagggcagtc tgtactgatg aaaaaaatat
     3901 tataagacaa tcaaaaaggc tttctctaga taaaatcaaa gaagataccg taaatataga
     3961 tggctttaat ttgataatat ccatagaggt agcattatct ggtggtactc tagtaatagg
     4021 aagtgatggc aatataagag atttagcagg tcttagggga acctataaga ttatagataa
     4081 aacagaagaa gctttgaatc tgataggtaa attttttaat aaatacaatg cacagaatct
     4141 taaattttat ttagatgctc cagtttctaa ttcaggaaat ttaaagtata ggatattaga
     4201 gcatgccaaa acttggggaa ttgaaactga agtagaatta gtaaaaaatg cagatgttgt
     4261 attggaaaaa ttagatagag tagttagtag tgatgcagtc atagttgaca agtgtataag
     4321 ttattttaat gtggctagag gtataattga agagtatatt aaagaatgta atatcataaa
     4381 tttaaataaa tagataaata taggtgagaa tatataagta tatgtagcaa tattcttagt
     4441 aatacaagta aagtttgaaa ataggagaac gaatacatat cgaatttcat aaaatgttgt
     4501 aaaaagacat atgagaggat ggatatgtgt gaaattaact aaactaaata ttaaaaaata
     4561 tagagcacct atatataaat atgctttagc aaatgtaaat ttgaggtctg ctaaatctac
     4621 taattcaagt atcataacag taatacctca gggagccaaa atggaagtat tggatgaaga
     4681 agatgactgg attaaggtga tgtacaattc tcaagaagga tatgtgtata aggatttggt
     4741 atcagtaagc gaatatgctt ggagtaactt aaacttaaga gaagataaat ctacaacttc
     4801 caatataatt acagtaattc ctgaaaaatc tagggttgaa gtccttcaag tagatggaga
     4861 ttggagtaag gttgtatatg atgataaaat aggatatgtt tttaattatt ttttatcaat
     4921 tgatgggaat aaaccaaatg aattagatta taagtatttt tatactgaca tgattaaatt
     4981 tgttaacgaa aataacatta aaagtactag cgattatcta attgtaacag accttagaaa
     5041 taaatatact tatattttta agaaagataa tggaggatgg ggacaacttt acaagtggca
     5101 atgtactata ggcaagcctg agactccaac tataacaggt atattttata taagtggaag
     5161 aaaaccttct tttggaacgg atgagtattc agttaagtat gctactagaa ttaaaggtgg
     5221 atattactat cattctgtac tatatgattc aacaggaagt tatataatag atggcagact
     5281 tggtgaagct cttagtcatg gatgtataag attaagtacg gaaaatgcaa agtggatata
     5341 tgataatata ccagatacaa caacagttat aattcattaa gaaaaatttt gagtttttta
     5401 ttgggataat aattcttaaa ttaatagaat tatatattaa cttctcataa agagtactat
     5461 ttaaaaagtg taataagaga gtgctattaa caaaacaaag atattttgag gcactctctt
     5521 ataaatatac ataattaaga tattttcata aacaattcta taatcttatc tttatcaaag
     5581 aaaacaggat tagcaccagc acaagcatct ttcatagagt tttctgcaag caattcaaaa
     5641 ttaggattat caacacctac atcttttaaa gattttggga ttccaacctc ttcagaaaga
     5701 gcttttatct tatctattac aaagtcaaca cactctttat ctgatttatt atttacatcc
     5761 attccaataa cttcagctat tggtcggaat ttggcagggg ctgatttagc attttcctcc
     5821 tcaactattg gaagtagcat ggcattacat actccatgag gtaaatcata taaacctcct
     5881 aattgatgag ccatagcatg tacatttcca agacctgcat tactaaaggc aattccattt
     5941 aaaaaacaag cataacacat ttgttctcta gcttctatgt cattaccatt tttaacagct
     6001 cttggaaggt atttaaatat ctgctttata gcatataagg cagttgaatc tgtaacatca
     6061 taagcaccat tagctactac agcttctata gcatgagtta gtgcatccat accagttgct
     6121 gctgttaaat tagatggttt gctaatcatt aattctgggt catttactgt cattgtggct
     6181 agtgaattgg tatcaaccat tatcatcttt acatgtctgt cttcatcagt tataacatag
     6241 tttattgtaa cttcagcaga tgtaccagca gtagtagtta tagctatgat aggaagacat
     6301 tttttagaag ttttgttgat accttcatag tctctaatat ctcctccatt agtggctaat
     6361 atagagatag ctttaccaca gtcttgtgga gaaccacctc ctatagttat aacgaagtcg
     6421 caattttctg attttaatat tttaagtcct ttattcacat tagttacagt tggattttgt
     6481 ttaacttcat cataaacaac aaagtctaca tctatctgag ataaaatatc tgttactttt
     6541 tttacagtac catttgaaac tagaaattta tcactaacaa taaatgcttt tttagttcct
     6601 aatgatttaa ttgaatcttt agcttcatta agacatcctt tcccaagtaa atttattggt
     6661 ggtacataat aaatgctcat aactaaaatc tccttttatt ttcattgatt tttatgtaaa
     6721 aatatatatc taacaatttt cactaactta ctcataatat atcgtatctt agattgagat
     6781 aggattgttg gttcttatat aagaacttaa ttcgtaatta cgattatgat tagaatttaa
     6841 catagtattt ttatcatgtc aataaaaata taaaaaatat tataaaaaaa acaaagttac
     6901 tgtagttaaa tcagcaactt tgtttttata gtataaatca aataaataca ttagtttaaa
     6961 acaaaatttt taattattat gttttgatat cttccatata cagaatatta atctaattta
     7021 ttacaatgtc ttcattacgt tcttccatca ttgattgaat atttttctga gcatttaaga
     7081 gtaatttttt tatcagttct attttttcgt cattagctct aaataaaggc agtgttacac
     7141 taacagcagc tcttatacat ccattttttc ttattggtac ggcaagacat tttaggtgtt
     7201 ccattgattc ctcacactct gaagctattt gtgttctttg aacttcaaga agctggtcgt
     7261 ataaaacatc aaaatctgta attgattttt ctgtaaaacc ttttagacca tctggatata
     7321 agcttattag tttttccttg tcaaaatcac ataaaagagc ttttccaaga cctgtacaat
     7381 tagcaggtat tttttttcca acataagaaa taagtctaat tggttctgga gaatctactt
     7441 ttccaatata aagaacttca ttattgtcaa gtataccaag ctgacaagtt tcagaacagg
     7501 aattaactat attagtcatt tcgtcaatta tatagttaaa aatagttctt ttttcaaaaa
     7561 atgaagaccc taatgtgtaa gaacgtatcc ctatagaata tttggatgtg tttttatcaa
     7621 tagtaatgta ttttctagca ttaagtgtat gaactatagg aaagatgcta ctttttggag
     7681 cattgataac atctgctatt tctgtaagag tatatccatt ttcggattct gctaatgctt
     7741 ctaaaatatc taatatccta gctgttgggc gatgattatc tgaagtcata aatttccccc
     7801 tctttttatt aatttaaagt attagaatat gttgatttta gcacactata cgatatttta
     7861 caatgtagaa aataaatatg taaatttata tataaataag ctcttgattt agatacagat
     7921 agatattttt ttagactgta cattaaggaa taactaattt ccaaaatgta cctaaagaaa
     7981 gtaagtgata tttagttata taatttaagt aaatatatgc aatagttatt tttatatgta
     8041 aatatcaaaa tattatgaac ttatatttta tctatttttt acactgtata aaaactttat
     8101 agttttgaat actatataat tttaaatacg ctgtattttt gcatataagg aagtcatttg
     8161 ataatataca aggtattatc agaaagtttg aaaaaatttt gacaaaaatt tatttttttt
     8221 attgactcat aaataggaaa atgctatcat taattcaaag attcgtaatt aaaaacttag
     8281 ttcttaaata cgaaccgaaa cggccgttag aattaaagct aaaaacttaa taagagtaat
     8341 taaatatcat gttttaggag gattaacttt atgataatgg attttattaa aaaaattcct
     8401 gcaggtatga tgatagtgcc aatgtttata gcagctttca ttaatacctt ttgcccagat
     8461 attgtacaaa taggttcgtt tacgacagca gtattttcaa gtgttgggtc tgcaactata
     8521 attggggttc aactggtatg cttgggaact cagcttcaat ttagagaaat gccaaaagtt
     8581 ttaaagagag gtggtatact tcttggttct aaatttgcta taggtgcttt gataggtata
     8641 cttataggaa agctatttgg aatggatggt gtccttggtc ttacaacatt ggctgttata
     8701 agtgcagtta caaatagtaa tggtagtata tatctttcat tgatgacttc atatggagat
     8761 gaaacagatt gtgcaactat gagtttactt actttaaatg atggaccatt ttttacatta
     8821 atagctcttg ggacttcagg tcttgcaaat gtaccattgt tgtcattatt ggctgcaata
     8881 gtgccaatac ttgttggtat gataatagga aattttgaca aaaaaatgaa agagttttta
     8941 gagccagggt gtaatttact cgtacctttt gtagggtttg cacttggtag tagcataaat
     9001 cttgcaagta tactaaaagg aggtttatca ggagtattac ttggattaat atctgtattt
     9061 gtaggaggta tatttatagt attctgtgat aaatttatag gaaaacgtcc aggatatgca
     9121 ggatgggctg tagctacaac tgctggaaat gcaattgctg tacctgctgc tgttgcatta
     9181 gttgacccat catggcaacc atatgttgca acagctacaa cacaagtggc agccgcagta
     9241 gttgtcactg caatattagt acctcttata actagttggt gggctaagaa gtatggatgt
     9301 cctaagttcc caaaacaaaa tgaggatggt acaataacag cttaatttat attacagtga
     9361 gttgttggta aaataataaa ttctttaatt agattttagg atattaataa aattatatta
     9421 tttataaagg agagcaagaa taatgataaa tgcagtgata attgatgaaa aagataatgt
     9481 tgcagtagct attgaagcaa tagctaaagg tagtgaaatt agttttaaat taaaagacaa
     9541 aacagtaaaa acgattacag cactagatga tataactatt tatcataaat tggctataaa
     9601 agatatggcc caaggtgata aagttactaa atatggtgaa catataggag aagcaaatca
     9661 ggagataaaa agtggtcaac atgtacatat tcataatgtt agaagtgtta gagaggattt
     9721 ggataaacaa gttatgacta atatataaaa taatatttat agagttataa aagttgtatt
     9781 cattgatttt agtgtcaaat agtttttaaa taggttttta gctgtttgat tagtgagtgt
     9841 aataaaaatt ataatatata aaaattatag aaagtatata gactatttta ggaggaaatc
     9901 agtatgaaat tttatggata taaaagacca gatggaagag ttggtataag aaaccacata
     9961 ttaatattgc ctacgagtgt gtgtgcatca gatacaacta gaataatagc atcacaagtt
    10021 caaggggcag ttacctttaa taatcagaat ggatgttcac aagtacattc agaccaacaa
    10081 ttaactatgg atgttatggc tggttatgca gcaaatccaa atgtatatgg tgtaatagtt
    10141 gtatctttgg gatgcgaaaa ttgccaaaat gatttggtag ttgatgcaat aaaagagaga
    10201 acaaataagc caataaaaac tcttgtaata caagaagaac atggaacttt aaaaactata
    10261 gaaaaagctg ttcgttatgc tagagaaatg gcacaagaag cttctttgct tagaaaagaa
    10321 gaatttccaa tatctgaact tatactagga actgaatgtg gtgggtctga cccaacaagt
    10381 ggattagcag caaatccatt aataggtgaa ttaagtgata aacttgtcga tttaggagca
    10441 acatcaattt taagtgaaac tactgaattt ataggtgcag agcatatact agctaggaga
    10501 gcagtaaatg aagaagtaaa agaaaaaata ttacatatag tacatagata tgaaaattca
    10561 ttaaaattgg ttggagaaga agttagagaa ggtaatccat ctcctggaaa tatagctggt
    10621 ggtttaacaa gtttagaaga aaaatcactt ggttgtatac ataaaggtgg tcatagacaa
    10681 ataagtgaag tatatgatta tgccaaacaa attgataaga aaggtcttgt tataatggat
    10741 acccctggaa atgatgcttc atctgttgct ggtatggtag caggaggagc acaaatagtg
    10801 gtatttagta caggaagagg aacaccatca ggaaatccaa tagcaccagt aataaaaata
    10861 actggaaata aaataacttt tgcaaatatg gaagataatc tggattttga tgctagtcca
    10921 gttatatatg gtccacaaac aatggaagaa ttaacagatg atttgttaaa tatggttgta
    10981 gatgtagcaa atggaaaaca atcaaaagca gagtcccttg gatatactga aatggcaata
    11041 gctagagtat gcaactatgt ataatcttta agtaagaaaa taaatacata gaattttaat
    11101 aaagggtgag attatgaagt ttgtaagttt tactgaagga gaaaatgaat gtaaaagaac
    11161 tggagttttt tctaaagatg aattatttat tatagatgtt aactctttaa atctcagtag
    11221 aaattttaag gatttaaatg aattaattca aaatgtatct agtgaagata ttattaagtt
    11281 gaaaactatt ataaatgaaa gtacattcta taattatgat gtttttaaaa gagagagatg
    11341 tactgtacat gttcctatag aaaaaccaat acatgatatc ttatgtgtgg gagtgaacta
    11401 taaagaccat cttgaagaaa ctcaaactca ctttgatgat agttttgaag aaccacaaaa
    11461 aacggtttat tttacaaaac gtgtgacaaa ggcaataggt ccagaagatg agatagatgg
    11521 tcattttgag ataaataatc aactagacta tgaagttgaa cttggagtag taataggtaa
    11581 aacaggtgtt aatattagaa aagaagaggt agaagattat atctttggtt acactgttat
    11641 aaatgatatt tctgctagaa agttgcaaaa agaacatgtt caatggttta gaggaaaagg
    11701 attagataca tttacctctt taggtccatg tattttacat aaaagtgcag ttccttttcc
    11761 cataaaactt aaagtatgta gtagggtaaa tggtgaagaa agacaaagtt ctaatacagg
    11821 gttattcata tcagacgttt cagacatagt ttcagaactt tcaaaaggta tgactttaga
    11881 ggctggagac attatagcta ctggaacacc ttctggagta gggatgggat tttcacctcc
    11941 tcgatatatg agtagtggag atgtagttga atgtgagatt gagagtattg gaattttaaa
    12001 aaattacatt aagtaactgt tttaatataa ttaatattaa agctaaatct gatgtattaa
    12061 tgaacatatt aaaataaatt ttaggagttt aaattatgaa agcagaattt ttgacaccag
    12121 ttgttacaat atttgatgaa gaaggaaatt tagataaaga aggtaataaa aaagtgtatg
    12181 accatcttat agatggaggt gtagatggac tagtcataat gggaagtact ggagagttct
    12241 ttaatatgtc tatggatatg caaaaagaac ttatagattt agtaactagt catgtaaata
    12301 agagagtaaa agtgtttata ggtacaagtc gtatgtgtat aaatgaatct atagaacttt
    12361 caaactatgc acataatagt ggagcagatg gtgttatgat aataagccct tattatttct
    12421 ctcttgatga tgatagtatt gagttatttt atagtgaaat agctaagaat acagaggctg
    12481 aaatatattt gtacaattat ccagatagaa ctggttatga tttaagtcca gaacttacat
    12541 taagacttgc aagaaaacat aaaaacatag ttggatataa ggatacagta actttgatgg
    12601 gacataccag agatttaata aatactatga aaatagagtt cccagaattt aaggtttata
    12661 gtggttatga tgaaaacttt atacataatc taatgtctgg gggagcaggt tgtatagggg
    12721 ggttatcaaa tttaattcca gaagtttgct ccaaatgggc tgaagcttac aataatagag
    12781 atatggagaa ggttatagaa attcaaaaat atattaataa agcaatggat ttttacttaa
    12841 taaccaatcc atttatacca gcaatgaaaa aagctatgaa tatcagaggg ttacaaataa
    12901 gtgattatat gactgctcca tttgtaaaac caaatgaaaa acaagtatgt gaaattaaaa
    12961 aattgatgaa tgaattaaat atatgttaaa tcaaccttgc aaagaggtca acaataaatt
    13021 acaattatct taataaaaat taaattaaaa taatactagg gtactatatt agtttaatag
    13081 ggaaagtgat gaaaattcac tacagccccc gctactgtat acggatacga aatcaaaatc
    13141 cactgaaatt tataaaaatt ttgggaaggg tgatggagga aaagccgtga gtcaggagac
    13201 ctgcccagta ttatataagt tcaatttagg gtggagattg taatttataa taaaatgaag
    13261 tatcaattcg tttttgtaaa gttgatatat gaacttctcc ttattaaatt aggagaagtt
    13321 ttttatttta ttaaaaggtt atacttatta aagatgactt taggataact ttaataagta
    13381 tactttaaaa agcatatatt attaaagttt tttagccact cataaaaaat aagatattgg
    13441 agtgaaagag atgaaattta aatcaaatgt tttaaagcag ttttttttaa ttgctatggt
    13501 aatttttatg gtaggaggtc ttatagggtg tagtaaaagt gacaaagaga ataaacaagc
    13561 tagtactggt aagaaattag aattaaaata tgctagtgga tttagtgttg aagatttaga
    13621 agatggaatt aaaaaagtaa ctgatggaga taatcgagaa ctgattttaa tcccaaagaa
    13681 gattaaaatt cctgaaaaat ataaaaatgc taatatagtt agaacgcctg tggacaatgt
    13741 attaatagca tctacaacac aggcttgttc tctaagaaca atagatgaac tagatagtat
    13801 aaaagccgta acaacacaag agcagtactg gactattaaa gaaattaaag atttaatgaa
    13861 agatggaaaa atacattacg ttggttcaaa tttagcacca gattatgaaa aaatagtaga
    13921 cttaaatcct gacttaacta tagtgagtag tggtttatca gaggctgata caaaatttat
    13981 tgacaaatta aaagaattag ggcttaatta tgttgtagac aatgagtata aagagcaaaa
    14041 tccatttgga agaatggagt ggacaagttt aatagctgca ttttatgata aagaagatat
    14101 ggctactagt gaactaaata aaactgtaca aaaggtagaa gaagtaagta aaaaaataaa
    14161 aaacaaagct aaacctaaag ttgtatgggc atctgtatat gaaggaagtg cttatatagc
    14221 tcctaaagat tcatatgtgg ataatatgat aaaaatggct ggtggaataa atgcatgtgc
    14281 ttcaatagat tcaaatgaag ggcgagtaag tattgaacaa ttgcatgaag ttgcaaaaga
    14341 tgcagatata tttgtatact catcaagttc agattatgca ccaaatttag attctataaa
    14401 atctagtgca cctgtattgg ctgatttgga tgttgtaaaa aacgggaacc tatgggtgtt
    14461 tgctccagat tattatcaat cagtagataa gacagacgaa ttaatagtag atttagttga
    14521 gatgttccac ccaggtacta caggtgaaaa aatcaaacat tatataaatt atgataaata
    14581 gtacagagag gacacaaaaa gataggtttt atagatttta gttaatatag ctttgctaaa
    14641 aggagtatac aaatggattt tatcaataaa atgagaataa ggaatacgac aatatttata
    14701 gtattaatta taagtatgat acttactttt ttagtgtgtg tagcatttgg ctctgtaaaa
    14761 attgaattta aagaagtaat agatgttgta ttaagaggta tggataatac agaacattat
    14821 aaaataatat acaatataag aatccctaga gtacttgcaa ccatggtagg tggagcatgc
    14881 ctagcagtat caggtatttt attacagata ttttttaaaa acccaatagt tgaaccgtat
    14941 atattaggag tatcttcagg ttctacttta tcagtagctc tcatgatttt gggaggatac
    15001 acattgggat ttgagagtgt aaattcaatg agtatatttg tagcagcttt tataggagct
    15061 ttaattgtaa tgtctatagt cattttattt gcaaataagg taaagaatat aacaactctt
    15121 ttaataatag gaattatgac cggatatgta tgtagtggta taacaaatgt gttacaatca
    15181 tttgcaaata gtgataaatt aaaagaattt actatgtggt ctatgggaag tttttctgga
    15241 tttacatggg aaaaggtaga aatacttata atagttggta tattaggtct tttgatgact
    15301 gtattcataa taaaaccttt aaatgctttt ttactaggag aagagtatgc aaaaagtatt
    15361 ggcataaata taagattttt tagagttttg atagtattta tatctagtat attagcagca
    15421 atagtatcag catttgcagg acctataggg tttattggac ttgcagtgcc acaaatttca
    15481 aaaatgatat ttaaaacatc agataataga gttttgatac cagtagtaat atttttaggc
    15541 agtatagtta cagctctttg tgatttaata tcaagagtaa ttttatcacc agtagaatta
    15601 ccaataagta caataacatc tttattagga gctccaattg tagtaatgtt attggtgaaa
    15661 aggaaggata gggcttagac atgttgaaaa ctaataattt aagtgtagga tacaataaca
    15721 aagttgttat aagtaatata aatgtagaag taaaaaatgg agaaatacta tgtcttttgg
    15781 gaagtaatgg agctggaaaa actaccctat taaggtcctt atctaaactt atatcaccaa
    15841 taaaaggtga aatatatttg aatggtgtaa acataaattg tatatcaaga aaagcattat
    15901 caaaaaaaat ggctttagta cttacaaata gattattagg tgatttaatg actgtacaag
    15961 atatagtcaa tataggaagg tatccatata caggattttt tgggagtcta tctaagaaag
    16021 atttaataat ggtagatgaa gcattggagt ctgtagatgc cttacattta aagaaaagat
    16081 attttgatga actaagtgat ggagaaaaac aaaaggtgtt ggttgcaagg gctcttgttc
    16141 aagaaccaga aataattata ttagatgagc ctacaactca cttggatata aaacatagat
    16201 tggaactcat aaacatactg aaaaaattaa gtaaagaaaa gtcaatatca gtaatacttt
    16261 cattgcatga aatagacata gcattaaaaa gttgtgataa agttgctctt ataaaaaata
    16321 ataaagtcat agcttatggc cagcctgaag atgtagtaga tgaggatatt ataaattcgc
    16381 tttatgaatt ggatgataaa aactttaata gtcttttagg ttcagtagaa attagtaata
    16441 aatctaaaaa tgaagttttt ataattggtg gtgggggtaa agcaacacca atttatagag
    16501 cttttactaa aaaaggtata ggtctgtatt ctggaataat acatgaaaat gatattgatt
    16561 atgaaattgg tagaactatg gggataaaga tgtttacaga aaatccattt gagcctataa
    16621 gtgatgagag tttcgatttg gctattagaa atttaaatga ttccaagatt ataatagata
    16681 caggatttag tgtaggagag acaaataaga ggaatataga tattataaaa gaagctttaa
    16741 aattggataa aaaagtttat tcttttagaa atagagatga gagtaagaag tattatgaca
    16801 gtttggatag taagatagaa catatagata aagtttctca aataatcaat agtgcagata
    16861 taaataattt attataattt atgaaataaa atgtacttat gtgttataga aaaaagagta
    16921 tagattataa tttttttgaa attacaatct atactctttt gttattttgt ttatgtattt
    16981 atatatttat aaaactttag cattttatag ataaccaata gcttcctctc tagttaaaaa
    17041 aaagtgaatc cctgtagtcg attctttcca tctattttca ttaaaatctt cagctacaat
    17101 cgtttctcct actctatata taaaattttc atcagcatat gcacgagcac ttttaaaaga
    17161 ttctttacca tctacactag taattgacag tacttttgcc ttatcacatc tacaagcatt
    17221 tgatgtagca gacactcttt ttgcgtcgct gggtattaat aattgtacca ttctaaaatt
    17281 aaaacatttt ttatagccta taaatgctcc tgtttcagga caatacaatt taaaaaaacg
    17341 agtactttca tctgatatta ctccatttaa agtagcatat tctaaattgg aattttctaa
    17401 attagctcct ctaatatctg agttagataa gtcacaaaat ctaaaacttg caccacttaa
    17461 gtctgcattt tttaatatag aattttttaa tgaacaatcc gaaagataag aattattaaa
    17521 aatagagttt tctaaatctg ctttttctaa atttaaattg ataaggtcag accacgaaaa
    17581 gtcaatatta cttaagcagt atcctcttaa gtccatattt tctaatctta attcttttaa
    17641 atctgccttt tctccagcat tattgttttt aagccataaa taatgtttct caagtgtttt
    17701 ttctaattct tgtttagaaa tagttttcat taaaatcacc ttcataaatt tatttttata
    17761 tatgtagtag caaaatagtc atatatttaa ttatataaca ataaataaaa aacttctaaa
    17821 taatatataa aatatcatct tgaaagtact tttttcaata tatcagaata tatttttaag
    17881 ctatttagag ataatttaaa tttttaaaca ggacataata agcttgtatt gttttatatt
    17941 tgagttgata aaaatttctt taaataaaaa taatgtcaat tataaagtaa taaaagttta
    18001 gatataaaaa aatttgtgta tatactatat gtagtatata atagataaag cactgactat
    18061 atgtagtgct tttgtttttg aaaaatactt attgcatttc tataattata gattaatttt
    18121 gggatttcaa ctgataagta ccaaaagaaa taataagcaa attaagtgaa attatggcac
    18181 aagattatgt taaaaggacg gtaaataatg aattggatag agcttaacaa tcaagtgata
    18241 attaaaaatg aacaaggcaa gtaccaattg gaaaaggata aggaagcctt aagtagttac
    18301 atagaagaat ttatacagcc aaagttgaga aaatttaata acctagaaga aagattgaag
    18361 tatttaatag atgaaggtta ttattcaaaa gaagtaataa atcagtatag catagatgtt
    18421 attaaaaata tatatgatat gattgataaa cataagtttg aatttcaatc atatatgagt
    18481 gcaaataagt tttatcaaaa ttatgcatta aagagcaatg atggaaagga aatattagaa
    18541 acatataatg ataaagtgtt aattgtagct ttgacactag gaaatggcga taaagattta
    18601 gctttaaact tggcagataa aatagtaaaa caagaatttc aaccagctac acctactttt
    18661 ttaaatgctg gaagaaaaag ggctggagaa atggtatcat gttttttact atctgtagaa
    18721 gatagtacag aaggtatatc gtatgctata tcttcatcaa atcacttatc aaagattggt
    18781 ggaggagttg cactaaattt atctaaactt agagcatctg gcgaatctat caaagatata
    18841 gaaggtgcag ctggaggtgt tgttggagta gctaaaatgt tagaacaatc atttagttac
    18901 tttaatcaaa tgggagctag acaaggttct ggtgcggtgt accttactgt attccatcct
    18961 gattttgaat tgttgatgga tactaaaaag ataaatgcag atgaaaaaat aaggcttgct
    19021 acattatcat taggagctat aataccagat aaatttatgg agttggcaga aaaaaatgaa
    19081 gtagcatatg cattttatcc tcatactgtg tataagaaat atggagtttc tcttgatgag
    19141 atagaaatgg ataagtggta tgataaactg gttaatgata gtgatataag aaaaaaagaa
    19201 ataaatccta ggcagatgtt gacaaaaata gcacagatgc aacaagaaag tggatatcca
    19261 tatgtagtgt acatagatac agctaataga gaacatacat taaaagatgt tggaattatt
    19321 aaaatgtcta acttatgttg tgagatattt caatatcaaa caccttcaga aatagagggg
    19381 tatggaggaa aaaatgaatg gggacaagat ataagttgta acctaggttc tttaaacata
    19441 gccaatgtaa tggataataa aactatagaa agtacagttg aaacagcaat aagagcactg
    19501 tcatttgtag cagatagcac agatataaaa ccagtgccaa cggtaagcaa tagtaacagc
    19561 aagtctcatt ctataggatt aggagctatg aatttacatg gatacttagt aagagaaaat
    19621 attttatata cttcagatga tgctatagat ttttctaatg tattttttgc aatggttaga
    19681 tactattcaa ttaaagcttc tatgaaaata gctatagaaa gaaatcaaac ttttgaagga
    19741 tttgataaat cagaatatgt taagggaaga aatagcaagg ttttatcaaa atactatgaa
    19801 caatcttatc tacctaagtc agaaaaggta agagcattat ttgaagggat ttatatacca
    19861 acaaaagaag actggacaaa attattggat gaggtaaaag aaaaagggat atataatgca
    19921 tatttaatgg cagtagcacc aactcaaagc attagttatg ttcaaaatgc tacatcaagt
    19981 ataatgccaa ttactgagcc agtagaagta agaacatatg gagactctac tacaatatat
    20041 ccaatgccat ttttaacaaa tgataatatg ttgtattatc aatcagcata caggatggat
    20101 atgagaaagg tcatagattt agtggcaaca gttcaaaacc atgttgacca aggtatatca
    20161 acaacactgt ttgttacaga tgaaaagaca actagagata tagctagaca ttacatatat
    20221 gcctataaaa aaggtttgaa aagtttatat tacacaagaa caaagatgac aagagatact
    20281 catgagtgtc tagtgtgttc agtataggga gagaaatata tgacatttaa tttacataaa
    20341 atacataatg cagttaactg gaatagagaa gaagatggat ttacacaagc tttttgggaa
    20401 caaaatgtaa aacaattttg gcttcctgaa gaaatatctg tttctaaaga cataaaggta
    20461 tggaatgaac taagtaataa agaaaaggaa ttatataaaa aagtattggg tggtttaaca
    20521 ttactggata caaaacaggg gaataatgga ataccatcta tgatgagttt aacagaaaac
    20581 ctacaaagaa aagctgtctt gtcttttatg gggactatgg aagaaataca tgcaaaaagt
    20641 tattcttcta tatttatgac tctactttct aatttagaaa tagatgaatt atttgaatgg
    20701 atagaaacag aaccaacatt acaaagaaag gctgatttag ttttagctca atacgaaaat
    20761 actacaaatc aagagggatt atacctatca atggttacaa gtgtattttt agagagtttc
    20821 ttgttttatt caggattctt ttatccattg tatctatctg gtcaaggaaa gatggtagct
    20881 agtggagaaa taatatcgct tattcttaga gatgagtctt tacatggtaa gtatatagga
    20941 ttgctagcac aagaaatata cgattcattt gataaaatgg ataaggagat gttagaagag
    21001 aagatgtatt ctatacttta tagccttatg gagaatgaga tagagtatac aaatgttata
    21061 tacagagaaa gtgggcttga aaaagaggtt gtgaactttt taaaatacaa tgcgaataga
    21121 gctttagaaa atctagggtt tgagaagcta tatactgtag atgctataaa tccaattgta
    21181 ttaaatggtc ttagcactga aaccaaaaca catgattttt tttcaactaa gggtaatgga
    21241 taccaaaaag gagtgtatga ggaattagag gatgaagatt ttataatata atttataatt
    21301 atgacacact gttgtcactt ttagcgtgat aatatcaatg tataaaatat atatggaggt
    21361 atttttatga aatatgaatg gagaaaaaga gataaagagt tatatttacc taaaaagaat
    21421 cctgtaatag ttgaaatacc agaacagaaa tttatcatgt taaaagggag tggagaccca
    21481 aattctaaag aatttactga agcaatagga gtactttatt ctttggcata tgcaataaaa
    21541 accatgccta aaaaaggaat tgtaccagaa gggtattttg actatacggt ttttccttta
    21601 gaaggtatat gggataaggg agaaaaaagt aaaggcttag atgtacttat taaagaagat
    21661 ttaatttaca ctataatgat acgtcagcca gattttgtta cacaagacct gttggataaa
    21721 gctactgata tagtaaagaa gaaaaaacct cataatcttc ttgaaaaagt tagatttggg
    21781 tctattaatg aagggctatg tgttcaaatg cttcatatag gttcatatga taatgaacca
    21841 gaaagctttg acattatgaa cgaattttgc aaaaaaaata aacttataag aaaatcttac
    21901 acacatcgtg aaatttatat aagtgatgct agaaaagtat cgcctgaatc attgaagacg
    21961 gttttacgtt ttgaagtaga aagggaagta taaatatttt gaataattta taagagttat
    22021 aaaaagtgaa gtctgttgag acttcatttt ttgatgcaat tttaaaatta aatttgatgt
    22081 ttcataatat tttaaaaact ttctactttt attagtgtga ataactgttg c
//
LOCUS       DAELTA010000055        21780 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.055, whole genome shotgun sequence.
ACCESSION   DAELTA010000055 DAELTA010000000
VERSION     DAELTA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.055"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            70..306
                     /locus_tag="KPG13_001749"
     CDS             70..306
                     /locus_tag="KPG13_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG0985482.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            319..1662
                     /gene="yqfD"
                     /locus_tag="KPG13_001750"
     CDS             319..1662
                     /gene="yqfD"
                     /locus_tag="KPG13_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG0985483.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1601..2716
                     /locus_tag="KPG13_001751"
     CDS             1601..2716
                     /locus_tag="KPG13_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG0985484.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            2773..3234
                     /gene="ybeY"
                     /locus_tag="KPG13_001752"
     CDS             2773..3234
                     /gene="ybeY"
                     /locus_tag="KPG13_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG0985485.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3240..3959
                     /locus_tag="KPG13_001753"
     CDS             3240..3959
                     /locus_tag="KPG13_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG0985486.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4078..4479
                     /locus_tag="KPG13_001754"
     CDS             4078..4479
                     /locus_tag="KPG13_001754"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG0985487.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4543..5436
                     /gene="era"
                     /locus_tag="KPG13_001755"
     CDS             4543..5436
                     /gene="era"
                     /locus_tag="KPG13_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG0985488.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5448..6827
                     /gene="mgtE"
                     /locus_tag="KPG13_001756"
     CDS             5448..6827
                     /gene="mgtE"
                     /locus_tag="KPG13_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG0985489.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6900..7055
                     /locus_tag="KPG13_001757"
     CDS             6900..7055
                     /locus_tag="KPG13_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG0985490.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7021..7794
                     /gene="recO"
                     /locus_tag="KPG13_001758"
     CDS             7021..7794
                     /gene="recO"
                     /locus_tag="KPG13_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG0985491.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7787..8386
                     /locus_tag="KPG13_001759"
     CDS             7787..8386
                     /locus_tag="KPG13_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG0985492.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8546..9424
                     /gene="glyQ"
                     /locus_tag="KPG13_001760"
     CDS             8546..9424
                     /gene="glyQ"
                     /locus_tag="KPG13_001760"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG0985493.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9424..11490
                     /locus_tag="KPG13_001761"
     CDS             9424..11490
                     /locus_tag="KPG13_001761"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG0985494.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(11656..13203)
                     /locus_tag="KPG13_001762"
     CDS             complement(11656..13203)
                     /locus_tag="KPG13_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG0985495.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            13483..14250
                     /locus_tag="KPG13_001763"
     CDS             13483..14250
                     /locus_tag="KPG13_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG0985496.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            14533..14730
                     /locus_tag="KPG13_001764"
     CDS             14533..14730
                     /locus_tag="KPG13_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0985497.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            14759..15835
                     /gene="vorB"
                     /locus_tag="KPG13_001765"
     CDS             14759..15835
                     /gene="vorB"
                     /locus_tag="KPG13_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0985498.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            15838..16557
                     /locus_tag="KPG13_001766"
     CDS             15838..16557
                     /locus_tag="KPG13_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0985499.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            16570..17109
                     /locus_tag="KPG13_001767"
     CDS             16570..17109
                     /locus_tag="KPG13_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0985500.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            17266..18324
                     /gene="buk"
                     /locus_tag="KPG13_001768"
     CDS             17266..18324
                     /gene="buk"
                     /locus_tag="KPG13_001768"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0985501.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            18342..19235
                     /locus_tag="KPG13_001769"
     CDS             18342..19235
                     /locus_tag="KPG13_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG0985502.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            19265..20473
                     /locus_tag="KPG13_001770"
     CDS             19265..20473
                     /locus_tag="KPG13_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0985503.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            20497..21756
                     /locus_tag="KPG13_001771"
     CDS             20497..21756
                     /locus_tag="KPG13_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0985504.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         8531 a         2386 c         4091 g         6772 t
ORIGIN      
        1 ctgttacaac atcaataaag tgcataaata cctatttcaa gcataaaatt aattaagggg
       61 gtaatgctta tgttagaaat atccactgat ttacaagtaa atcaaccagt tattactgtt
      121 acaagtaata cgtttattag tatagaaaat tatctatcaa tattagaata tgaagttgat
      181 ttaataagaa taaagactaa agttaaaaca attaaaatat ctggggataa actttcactt
      241 aagtatatta ctgatagtga gataggtata aaagggatta tctacaatgt tgagtatgtt
      301 gattaggagg agatacactt gataagtttt ataaggggat actatgttat agttgtggaa
      361 ggagtaggac tcgaacaatt tttaaatcat cttataagaa atgggataag tgtttacaat
      421 gtaactagaa taaaaaatac taaaatggag tttcatatag atagacaaga tataaaagac
      481 tttaagaatg tttatagagg aagtaagttt gatataaagg taaagcaaaa aactggggtc
      541 ccttttatta tcaaaagagt ttataagcac aaaggtatgt ggatttgtgc tcttgtttct
      601 ttatttttat tgatgtctac atcacagttt gtgacagatg tatatataca atctcctgaa
      661 ggtataaaaa aagaagctct tagaaatgag ctttataagg ttggagtaag gccaggagtc
      721 tataagaaga gtattgatag aaaagaagtt agagaccata tgatgtcaaa atttaatgat
      781 gtggcatatt tatctatcaa tgtaaaagga acaaatatat ttgttactgt tacaaaaaag
      841 gcagaatccc taaaatctac agaccaatca aattattgta atgtaattgc tttaaaaaat
      901 ggtataatag aaaaggtaat ccctagaagt ggtaaatcag ttattaaatc tggagacata
      961 gtccaaaagg gagatgtctt attaaatgga gctaatacca agtcaatacc agaagtatgg
     1021 gcaagtactt tttatgagtc cacaaaaaag gcaagttatg tagatactgt aaataaaaaa
     1081 actggagaaa agaaaaatat atatacacta agtttttatg ataaagagtt tacaatgaga
     1141 aaaaatataa aatataaaga ttatgttgtt gaaaataaag aaaaaaaact atcaatagga
     1201 aactatactt ttccaataaa gataaaaacg agtacttttt atgaaacaaa aaaggttgaa
     1261 gtaaaaagga ataaagaaga gttaaaaaaa gaattatctg aaaaagcttt aaaagagtta
     1321 gaatacataa tcccagcttc ggctagaata atagatgtaa aacataactt taaggttaat
     1381 aaaaatatgt tagaatattt aataacagtt caaactagtg aaaatattgc taaaatttat
     1441 cctcttagta agtcagaagc agaaaggttt ataaaggaag aaagtaaacc agatgaaggt
     1501 gaagaggaag ttccttccaa tccagaaaaa agaccattaa atgatataag aaatgagttt
     1561 gatgaggata ataaggataa taaagaccaa aacaattctg atgaaaataa tagtaatcaa
     1621 aatagtaata atagtcaaaa taataatagc aataacaact aataaaatta aacttaaaat
     1681 cttaggagga ctaattcaat tgataataca aaagaaattt acaatagcag atggtaactt
     1741 tgaaagagag ttatttggca actttgatga aaatgtaaaa ttaatagaga agactttaaa
     1801 tatagatgta atacttagag aaggcaatat aattttaatt ggagaagaaa aaaatgtaga
     1861 ttctgcatta aaacttatga atgaattaca ccaaactgta tcaaatggta agcatttaga
     1921 taagcagagt atttcttatt cattatctct tttgcttgaa ggaagtgaac aaaaaataaa
     1981 agaacttgag gggacaatcg tcataactca aaggggaaaa gctgttcaac ctaaaacttt
     2041 aggtcaaaaa gaatatataa aacttataga aaataatgat ataacttttg gtgttggacc
     2101 tgctggaaca ggaaagactt atcttgcggt tgctatggca gtaaaagctt ttaaaagaga
     2161 tgaagttagt agaattatac ttacaagacc agctgtggaa gcaggagaga gtctaggttt
     2221 tttaccagga gatttaaagg acaaagttga cccatattta agaccactat atgatgcctt
     2281 atttgaaatg ttgggtgctg ataaatttaa taagtattta gaaagaggta ctattgaggt
     2341 tgcaccactt gcatttatga gaggtagaac acttgataat tcatttataa tattggatga
     2401 agctcaaaat actacttctg aacaaatgaa aatgttttta actagacttg gatttggctc
     2461 taaagctgta gttacaggag atgtaactca aacagattta ccacaaaata agaagagtgg
     2521 tctaatacaa gctacagaga ttcttaaagg tgttccaggt attgggtcta taatgctaac
     2581 agatagagat gttgtaagac atgagttggt acagagaata ataagagctt atgagaagca
     2641 cgacaaaaga gaagaattta aaaaagaaga acgtaaaaaa gcgaaaatgc aggaaaagac
     2701 tttcaaaaga aagtaaaagt gtagtaatat agatatatag cattattaat gaataaaaag
     2761 taggtgtagt gaatggattt gatactagat gatagacaag ataaattaga agtaagtgag
     2821 gagttaattg aaaagataaa ggatataatc atagaatgtt tagattatga aggatatgat
     2881 gataattatg aagtgagtct ttcttttgta gataataaag agattcatga attaaataga
     2941 gaatatagag gagttgatag agtgactgat gttttatcat ttcctctttt aagtgatgat
     3001 tttgaagatg ttgagttaga agaagaatcc ctaggtgaca tagtagtatc tcttgaaaga
     3061 gccttagaac agagtataga gtataaccat agttttgaaa gagaagtatg ttttttaata
     3121 tgtcatagta tgtttcatct tttaggttat gaccacgata cagatgaaaa tacaaaagaa
     3181 atgagagaaa aagaagaaca catattaaat aagttaaaca taacaaggga gtagttttta
     3241 tgaaaccaga aaaaactcgt caaggtataa ttaaagcatt taatgcagct atagaaggga
     3301 tattgtatac atttaaattt gaaagaaata tgaaaataca ttatttaggt tcagttgctg
     3361 ttcttataat aagtttgttt tttaactttt caaaattaga aatgataatg ttacttatgt
     3421 ccatatgttt agttgtagtt gctgaaatgt ttaatacagc tatagaaaaa gctgttgatt
     3481 tagtaactga tgaatatcat gttcttgcta agatagcaaa agatgttgca gctggaggcg
     3541 ttctagtggc agcactaaat tcagtagtag ttggatatat tttgttttat gataaattga
     3601 cagatatatc tggaatatta atttataaaa taagagaatc agaattacat ataaccttga
     3661 tatgtatatt attggttctt atagctgttg tagttgtaaa agcattgact tctacaggta
     3721 ctcctctaaa aggaggaatg ccaagtggtc atgctgcctt agcatttgct atagcaactg
     3781 caattacact tatgactgaa agagttgttg cctctactct tgcatatata atggctgtat
     3841 tagttgccca aagtagaatt gaaggaaaaa tacatacatt ttgggagact attgctggag
     3901 ctttgttagg tgtgttgata gctatattag tatttcagct tggaatgttc tataattaaa
     3961 ataaatgtac ataaaatatt taaaataaaa caaatctgtg ctaattaaat ttttatgata
     4021 taatgtagag tattaaataa aaataacttt taattattgt atacaggagg agttgttgtg
     4081 gataacatag aattgttaag gttggcagaa gatgccagac aacattctta tgctccttac
     4141 tcaggtttta gagtaggtgc agctctttta actaagagtg gtaaagttta tactggatgt
     4201 aatatagaat gtgcatcatt aggtggaaca aattgtgcag aaagaacagc tatttttaaa
     4261 gcaatatcag agggtgataa ggatatctat aaaatagcta tagcaagtga taactcagaa
     4321 aataatgagc aaacatatcc atgtggaata tgcaggcagg taattataga gtttggttct
     4381 gatataaaaa taataacggg atatactaaa ggagaggtgt ttgagcatag tataaaggat
     4441 ttgttgccaa actatttctc tggaaatgat ttaaaataaa tttaaaagta taaataaatt
     4501 taagtaatta aaacaagaag ataaaaagtt aggagacagt taatgttcaa atcaggtttt
     4561 gtcagtatag tgggtagacc aaatgtagga aaatctactt taatgaataa tgtagttgga
     4621 gaaaaaatag ctataatgag tgataagcct caaactacaa gaaatacaat acaagcagta
     4681 tatacagatg aagaaatgca gatagtattc ttagatacac caggtattca caaaccaaaa
     4741 aataaattag gtgaatttat ggtaaaggca gcaacagaag cttttaaaaa tgtagattta
     4801 atattatttg tagttgatga ttctaaaaaa ataggtcctg gagatagaaa gataatagag
     4861 gatttaagaa gtgttaagac accaataatt ttagttgtaa acaaaataga ccaactagac
     4921 caaaaagatg agctatttga tataatcaaa atgtatgata gagaaggtat ttttaaagaa
     4981 atagtaccaa tatcagcatt aaaaggaaag aatacggata ccttaataaa agttatacaa
     5041 aattatttag aagaagggcc aaagtatttt ccagattata tgattacaga ccagccagaa
     5101 agagtattaa tagcagaact tataagagaa aaagttcttc actatcttaa tgatgaaata
     5161 cctcatggtg tagctgttga aattgaaaaa atgaaagcga gaaatgataa ggaaatagta
     5221 gatgtatctg ctgtaatata ctgtgaaaga gattcacata agggaataat aataggaaaa
     5281 aatggtagga aactaaaagg aataggtaag tcagcaagac aagatataga attgctttta
     5341 ggttcacaaa taaatcttca attatgggtt aaagtcaagg aaaattggag aaatttacaa
     5401 aattatataa ataattttgg atataatgat aaataaaggt gggttatatg gataggaaac
     5461 tggatgcttc ccaagattta ttatatgaag taaaatcatt aattgataat aataaagtat
     5521 tggagttaag agaattaata gaggaatatc atataattga tatattcgat ataatggaaa
     5581 atttagagga agacatgaaa attcaattat ttgaagtact tcctttagat atggcatctt
     5641 ctatattaga agaaggtagt gtagagtttt ttattagtat tttatctaaa ctggatgtag
     5701 aacactcgaa aaatatactt gaacttatgt ctcttggaga tatggcagac aagttgagtg
     5761 aacttgaaga agaagaaaga gaacacataa taaatctttt gaatcaagag aatgccgatt
     5821 atgtaaaaga gttactattc tatgatgaag attctgctgg tggaacaatg accactggat
     5881 atatttctat aaataaagat atgacagctc ttgaggctat agaccatatg agagaagaag
     5941 ctgaagaagc tgaaactatt tattatatat atgtggttga tgatgaagaa aaactagttg
     6001 gagtattgtc attaagagaa ttaataatag ctagagacgc aaatatagta gaagatttga
     6061 tgagtgaaaa tataatctct gtttatgtag atgaagatag agaagaagca gtaagattag
     6121 tatctaaata caatttaata gcaattccag tagtagacag acaagaaaaa ttaaaaggaa
     6181 taataacagt agatgatata attgatgtaa tggaagaaga agcaactgaa gatatgtata
     6241 aatttgcagg aagttcagaa catgaaagag aagttgcgga aaaagaaaac cctacattga
     6301 gagagcaaat aatatcagca cttagaggac gattaccttg gttaattatt accttagttg
     6361 gagggttgtt ggcttcgtta atactttcta acttagacta tataatgaat cctgtatatg
     6421 ctccattagt attttttata cctgtagtaa ttggtatggg tggaaatatt ggaactcaat
     6481 catcatctgt tacagtaata acattgtcta ataaggattt aaattttagc aatgtggtta
     6541 gagaaggtat tgtaggtatt attacaggtc ttttgtgtag tataataaca ggtattgtaa
     6601 tttactttgt tatgagaaag ttagacattg tattaattgt ttcaatatca ttatttataa
     6661 atatggtttt aggtgcaaca ataggtgcgt ttatgcctgt tttattaaaa aaaatggatg
     6721 cagacccatc tacagtttca tcaccaatta tatctacagc tcttgatata actggtatag
     6781 cagtttactt tataataaca acggcattat tgtcaaaaat tgtttaataa ctttatgtat
     6841 gatttatcct cttcttacta aaaatataag tataaatgta agaagggggt tttttaaaaa
     6901 tgaacaatat cagttgggaa gtttttaaaa aaacaggtag tatagatgca tatttatatt
     6961 tctgtgattg caaaaacata catgaagaaa ttgaagaagt aagggagaaa aaagaagacg
     7021 atgataatcc tgaacaccca ggggatagta cttaaagcta taaggtataa ggaaagtgat
     7081 ataatactta cactatttac aagaaagctt ggaaaagtat ctgcaatagc aaaaggagct
     7141 aaaaaaaata agagctcttt gctttcatca tcacagttat tttcttatag caattttacc
     7201 ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg aaataataaa aagtttttat
     7261 aatatatcct atgatataga ggcattttct tatgctacat atattacaaa attagttgaa
     7321 aattctatac ttgaaaatca gactaataat aggctattta tattacttgc gcagactctg
     7381 tatttgtata cacaggataa tacagacaat aggtttataa cagcagcatt cgagttaaaa
     7441 tttttagact atataggatt taaacctata gtaaataaat gcattaattg tgaatgtaaa
     7501 attttacaaa attctgtgtt taatatctac gaaggtggta tactatgtag taagtgtagt
     7561 aaactttttg ataataatat aaaattagac ttaacaacta taagtcttat ggaatatgta
     7621 ttaagaaatg atattttaac atgtagtaaa gctaaagtat caaaatatat aacacatgaa
     7681 cttgagaata ttctagacaa gtatttgaaa gtttatgttg ataatattaa ttttaaatca
     7741 ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg ataatgatga gtagtaatat
     7801 aactattgaa atggttgatt cagtctttga aagagttcca ggagcaactt atgcagaagc
     7861 aaaagaagca ttagtaatgt gcaatggaga tgtaattgag gcagtaatat atttagaaaa
     7921 taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa gaaactatgg aagaagtatt
     7981 tggcaaagat ggagaaaata taaagtttca attaaaagag cttcttagaa aaagtagtgt
     8041 agtgcgaata atagttgaaa aagatggaaa aactatgatg aacattcctt taacagttgg
     8101 agttgttgga ttagcttttg taccattagc tactttagtt ggattatcag ctgctgttat
     8161 aagtaaatat cgtataaaag ttcaaaatga agaagatgga gaaattgtag atttaggtga
     8221 attgaatgaa gaaaaacttc acgttttgaa gaatatgata actaatgcag caaaagatgt
     8281 gaaggatgta gtagttgaca aaaaagaaga tgacaaagat gtaactgctg accttatgaa
     8341 agaagaatct gaaaagaata aagaagacga agacgaaaat aaatagatat aaagtgactt
     8401 ttaaaaaatg aagagaagat aattataaga attatcacta acattgacaa attttataga
     8461 atttgatatt ctaaaagttg tagaagaact attaaaatag cctttcgtgt agacgagagg
     8521 ctttattgtt ataggaggga tttttatgaa ttttcaagag atgatactgg cgttgcaaaa
     8581 atactggtca aagcaaggtt gtatcatgat gcagccttat gatattgaaa aaggtgcagg
     8641 aactatgaat cctaatactt ttttaagatc attaggacct gagccttggc aagtatgcta
     8701 tgtagagcct tcaagaagac cagcagatgg aagatatggt gaaaacccaa acagactata
     8761 tcaacatcat caattccaag ttatactaaa accttctcca gataatatac aagaattata
     8821 tttggaaagt ttaaaagaga ttggaattga cccaagtgag catgacataa gatttgttga
     8881 agataactgg gaagctgcta cagttggtgc ttggggactt ggatgggaag tttggctaga
     8941 tggtatggaa ataactcaat ttacgtattt tcaacaagta ggtaatattg aatgtgagct
     9001 tgagacagga gaaattacat atggattaga aagattagct atgtacattc aagaagttga
     9061 tagtgtttat gatttaaaat ggaatgataa aataacttat ggtgaagtat ttaataaagc
     9121 tgagtatgaa aactcaatgt acgcatttga attatgtgat gcagatatgt tgtttaatct
     9181 gtttgatata tatgaaaaag aagcattaag acttatggaa aatggccttg taataccttc
     9241 atatgactat gtgttaaaat gctctcatgc atttaataca ttggatgcaa gaggtgcaat
     9301 aggagttagt caaagagctt catttatagg tagagttaga aatatggcaa agactgttgc
     9361 agaaactttt gtaaaacaaa gagaagaaat gggattccct cttttaaagg acggtgacaa
     9421 ataatgaaaa attatctttt atttgaaatc ggtgttgaag aaatgccatc aagatttgta
     9481 ggcagtacac tagaacaatt aaaaaacaat cttagtaaat tatttgatga aaatagaata
     9541 gcttttgata aaataaatgc gtatgggaca ccaagaagac ttactttagt agtcgaagga
     9601 ttgagtgata gacaaaatga tttagaagag gaaataaagg gtcctgctaa gaaaattgct
     9661 gttgatgcag aaaataattt aacaaaacca gcattaggat ttataaaaag caaaggatta
     9721 agcgaatctg acatatattt taaacaagtt ggaaaagaag aatatatatt tgcgactata
     9781 aagcaagatg gaaaagaaac ttcagaagta ctaaaagaca tattaccaga gactataaaa
     9841 tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa atcttaaatt tgcaagacct
     9901 ataagatgga tagttgcatt gttaaatgat aaagtattag agtttgattt agaaggaata
     9961 atatcttcta atgtaacaaa aggtcataga tttttaggtg aaagtacaat agaagttgat
    10021 tctatagaag attattttga aaaattagaa aaaaattata tagttttaga ccaaaacaag
    10081 agaaaagaaa tgataagaaa acaatgtata gaagtggcaa attctttagg tggagaagtt
    10141 gaatttgatg aagatttact tgaagaggta acttacttag ttgaatatcc aactgctttt
    10201 tatggagaat ttgatgtgga ttatataaaa ctaccaaaag aagttgttat aactcctatg
    10261 aaacaacatc aaagatattt tccagtactt aaagaaggaa aactgttacc taactttata
    10321 gctgttagaa atggtgattc ttacagaata gacaatgtta aagctggaaa tgaaaaagtt
    10381 ttagaagcaa gacttgcaga tgctttattt ttctatagag aagatactaa gagaaactta
    10441 gaaagctaca tagaaaaatt aaaaactgtt gtattccaag taaaactagg aactatttac
    10501 gacaaaactt taagacttga aaaattaagt gctgatatac ttgatagttt aggtttagta
    10561 gatgaaaagg cagatactat aagggctgct aaattatcta aggcagattt agttactgga
    10621 atggtaggag aatttgatga attacaaggt tttatgggaa aagaatacgc aaaagttgga
    10681 ggagaaaatg atgttgtaag tgaagctatt tttgaacatt atcttccaag atttgcagga
    10741 gatatacttc ctaagactaa tgcaggagtt gccttatcaa tagctgataa gttggattca
    10801 atagcaggtt tctttgctat aggaatacaa ccaacaggtt ctcaagaccc atatgcttta
    10861 agaagacaag ctttaggtat acttagtata cttatggaca gaaaagttgc tgtggatata
    10921 aaagtactta ttgaacatgc tttagacaat tattctgaat tagattttaa taaagaagaa
    10981 gttgttgagc aaataatgaa tttcttcaat gagagagtaa aaaatctatt taaagattta
    11041 ggaataagat atgatgttgt tgatgctgtt ttaagttctg atataaatga tgtttctgat
    11101 atgcatatga gagctttaga gcttaataat tggctagaaa aagatgaatt agtggagatg
    11161 cttactgcat ttaatagagt ttctactcta gctcaaaaag cagaaagtga taaaattgat
    11221 gaatctttat taaaagaaga tgctgaaatt aaactttaca atgaatttaa acaagtaaag
    11281 gtaaaagtta atgaattatt aaaggataag aaatatggta ttgctttaga tgaatttgca
    11341 tctttaagac cagtagttga taatttattt gatacagtta tggttatgga taatgatgaa
    11401 gctattaaga acaatagact tgctttactt aaacaagtat atgatactat gctaggaatt
    11461 tgtgatttat ctaaaattgt ttataaataa aatttaaact atttataaga aataaggctg
    11521 tctcaaaata gagatagtct tatttttcat tgttgagatg atgaaaatat atttgatttc
    11581 atagaaaaag agtgtctcat aaactaaaaa gttcattttg agacacccat gctttttgta
    11641 agtaaatata ataaattata ttttatactt atcagttatt ttgtttaaaa tattatcatt
    11701 tgaatttacc cattcataaa aattgcctga tgatgcgtcc gatattttat aaagcatttc
    11761 aggaatatca ctagctttaa aatctccaag tgattttccc aatctagtct tacctatttc
    11821 aaaactgcct ccaacaaata attcaaatgc atctgtagat ataccatcaa cattcttctt
    11881 tttaccacat aaacctatgc taccaacttg atgaacacca catgaattta aacatcctga
    11941 tatatataac ttaggtgctt tatttaaaat ttctttatta ttttgtaatc taaaataatc
    12001 tatgatttca tgtaacattt tctgactatt ttgtatgccc atttgacaaa ttggaacacc
    12061 tatgcaagat atactttgtt ctaattgaga attacaacta atagttttac ttatttctaa
    12121 tattcttttt gcttcatttc catttaaatt taaaatatat aatccctctg tcattcctag
    12181 tctaatcata ggattttcaa tattatctaa ttctttaagt aaagtactta aatcctttgt
    12241 taacaaaata cctccaactg gatgtatata tacagtgtat aaaccatcct gtttttgagg
    12301 tattagtcta gaatcctgta tatctatttt aaccccaggt ttagagtaat ctatttcttc
    12361 aatattgagt tcaagactac cattttcttt ttctaattta tagtattctt taaatttttc
    12421 taagaataat ttttcaccaa gtgattcgac catatatcta accctagctc tgtttttatt
    12481 ttcataattt ccataatcca taaagaattt aatcatacct tcaacacaat ataaggcatc
    12541 tttcggtttt ataagttcat caagttctaa agcaactttg ggttgtttac ctagacctcc
    12601 acctaagaaa actctgaaat atggttcatc atttttcaaa gtagcaacaa atcctaagtc
    12661 ttgtatacta caatgagcag tatctgtgta acaattagaa taagaaactt ttaattttct
    12721 aggtaaatgg tatgtagtag catttttgat aaaatattta tcagtagcaa ctgcatatgg
    12781 agtaacatca aagacttcac tagggtcaac tccagataat ggagataatc cgacatttct
    12841 aggataatta ccaccaccac ctctagtgaa tatatcattt tttattcctt cttccatgat
    12901 atcaacaatt gaatttatag ataaatcgtg taattgtata gcttgtctag ttgttaagtg
    12961 tatcttatct aagttgtgtt taatagctat ttcatagatt ttattaagtt gatactgaga
    13021 taaaactcca gcagatactc taagtcttat cataaaagat tttttgtctc tttgagcata
    13081 aacaccatat ccaccagaaa atcccttata ttgcatttta tttatttctc catttaaaaa
    13141 ttttgaagaa tattctttaa aattaggtag ttcatttaac aatatctttt taaaattatc
    13201 cattatttca cctcattgtt agtcttgtat aaaaatatat ctattgtata atagatatat
    13261 atattattat ctagtcagga taaaaatata acagaataat tctattaaaa gtaagaataa
    13321 taaggacata gtgagtattt tgtttttatt ttacaaatta agtataatat ttttgtttta
    13381 taaaatataa ttaagataaa tagataattt tgtaatttta atatataata tttagtagag
    13441 tgtgggagta gcacttaata tttgaaggag gagtttttgt atatgggaga aataataaat
    13501 gcattagata gagccctaga cataattttg ctgttatatc atgaaaagag agaaatggga
    13561 ataactgaaa taagcaaggc tatgggagta tataaaagta ctgtacatag aactttggtt
    13621 actcttgaaa acaaaggttt tgttatacaa aatgcagaga atggtaagta ttggctagga
    13681 ataaatttat atgcaatagg tatggttgtg ggagaaaaaa tgtcattgac tgaaatagta
    13741 aaaccatata cgaaaaaatt aaatcaagaa tttaacgaag tagttaatgt atctatatta
    13801 gaagaaagag ctcaagattc tccaagaagt atcataatac ataaagagta tggttcaaat
    13861 caattacttt cagtaaatcc atccgttggt tcaagtagtg aatgttattg ttcagcagtt
    13921 ggtaaatgtc ttatggcatt taatgattct atagattttg aaaaatatag aaaaacacca
    13981 atacacaagt atacagaaca tacaattgat aattgggatg atatgatgct ttttttagca
    14041 aaaataaaag aacaaggata tgctatagat gatgaagaac tagaacatgg actaacttgt
    14101 attggagcac ctatcttaga taaaaataat aaagctattg cagccataag tttgtcaggt
    14161 ccaactataa gaatgagaga aggggacttt gagtataaga ttaaaagagt tatagaaact
    14221 gcaaaaagta tatctgaact ttttagatga tttttaatct cgataataaa aataggctat
    14281 atttaaattg ttatttgtaa atatcagttt aaatgtagcc tatttttctt taatctaaaa
    14341 ttaaatattc ttaaaattta aaatgttatt gaaatatata agcgaatgtg ttatctttta
    14401 attaagcaat aaaaatagaa caaggttcta tactatagaa caaaattgat gcatatatag
    14461 tatttataca aaatataaat attatatatt taaaattaag taaaaatatt acataaaggg
    14521 gagatttgag aaatggaaaa agtcaagttg agtgttgaaa gatgtaaggg atgctatctt
    14581 tgtattgaag cttgtaaaaa agatgcaata agtctttctg actacacaaa taaaaaaggc
    14641 tttgtaacta ttaaagtcga tgaagaaaaa tgtattcaat gtggcgcttg ttatacaatg
    14701 tgccctgatt tagtatttga gatattataa attattttta ttagagggga gaaaaggtat
    14761 gcaaagttta ttaattaaag gaaatgaagc tttagcagaa ggtgctatac gtggtggctg
    14821 cagattttat gcaggttatc caataacgcc acaatcagaa atattagaaa tgatggcatg
    14881 gagacaaaaa gaagttggag gagtatttat ccaaggtgaa tcagaaatag ccagtgtaaa
    14941 tatggttatg ggagcaagtg cacttggagc aagagctatg acttcttcat ctggtccagg
    15001 attttcatta aaacaagaag gtattgcata ttgggtatca gctggtttgc cagcagttgt
    15061 agcatgtgtg caaagatatg gtataggtga tggatttata ggagctggtc aagataatta
    15121 ttggcaagca gtaaaaggcg gtggaaatgg agattatcat ttaatcgtgt tagcacctaa
    15181 tagtgttcaa gaaaatatgg atatggcata tgaatctttt gaattggctg aaaaatatat
    15241 gcatccagta cttatacttt ctgatgctac aataggtcaa atggtagaac cttgtatagc
    15301 accaccaatg aaagaatacg atatggataa agctccatgg gctgcaaaag gaacacctgt
    15361 aggagaaaaa aataaagtaa taactgatat aacatatttt gaagaccaag atacatggca
    15421 aaaagggtat atagaaaaaa tgagaaaagt tcaagaagat gaacaaagat gggaagagat
    15481 agaaatagaa gatgctgaag tggtatttgt ggcatacggt atttcatcaa gaatagcaga
    15541 aggtgcagtt agagatgcta gagcaaaagg aatgaaatta ggtctaataa gaccaaaaac
    15601 attgtggcct tacccaagaa aagcatttaa gaaattaagt gattcattga agggacttgt
    15661 aacagttgaa atgaacatga tggcacagat gaaggaagat gtaattatag catgtgataa
    15721 tagatttcca gtatattcag tagcaacagc tcaatatatg gcagaagtaa atcaattaat
    15781 agattttgct caacagatta tagatggaaa agctaagcaa gaggaggtat tttaattatg
    15841 gctaaaaaag caccagcaat aatgatgtgt gaaaatatgt tctgtggagg ctgtggacac
    15901 tctatagtcg atagaattat tggagaagta ttaacagaaa tggatatatg tcaagataca
    15961 attatatgtt cagatatagg ttgtaatcat atgtttcaat ttagtatgaa tgtagatgtt
    16021 gtcgttccac ctcatggtaa aatgggagca gcaatggcag ctatgaaaag agttagacca
    16081 gataaatata tatttacaat agctggagat ggtggagctt atgcaatagg gcttggagaa
    16141 actatgtcag ctgctactag aaatgataat gtaatatttc ttgttatgaa caataatatg
    16201 tttgcaatga caggagggca aatggctcca actactatag aaggtcaata tacagcttct
    16261 actccagcgg gtagaaatta cgcagatcat ggttcaacat ttgatgttgt aaaagttatg
    16321 ggaaatttag atatagcata tctagcaaga ggaacaataa ctaaccctaa agaaataaat
    16381 acaacaaaaa gatacatcaa aaaagctcta gaaaaacaaa aagaaaagaa aggtttttgc
    16441 ttaatagaag tgcttgtacc atgtccaaca aactggggat taaagccagt agatgctatg
    16501 aagagaattg aaaatgaact tgttccaaag tatccacttg gagaatttat tgattaggga
    16561 ggaaataata tgttgaatga aattatatgt gctggttttg gaggacaggg agttcttaca
    16621 gcaggaaaaa ttataatgta tgttgcatat aaggcagaaa agaaaattac atggttccca
    16681 tcttatggaa atgagatgag gggaggaagt gcaaattgca atgttgttgt aagtgatgaa
    16741 aaaattgcaa atccttgtgt aaaaaatcca gatatactat tgggaatgaa cagcgtatca
    16801 gtagataagt atgaaagttt aatgaaaaaa aatggttata tgtttgtcaa tgaatcacta
    16861 gtaagtaagg atagaaagta cagagaagac attaatgtta taaaagtacc tgttactgaa
    16921 atagcacaaa atttaggcaa tgaaaaagct gctaacattt gcatgttagg tgctatggta
    16981 aagagtttgg atttatttac aaaagaagaa tttgaaagtg gtatgtgtga atactttgaa
    17041 aatcaaggca aaggtaaatt caattctaaa aatgtagaag catttgaagc tggatacgac
    17101 tacgaatgaa tattttaaat atttaaaata attattgaaa tacttatttt tatatgttag
    17161 tatttaatta ataaaatata gaacgtcgtt ctggacaatg ggacgaagtc tttaggtcgg
    17221 aatacaagaa cggcgttctg tataatagaa ctagggggtg ttaaattgaa agttttagta
    17281 ataaatcctg gtggtacatc cacaaaaatt tctgttttcc aagatgaaaa tgaaattttg
    17341 aaaaaaaata taacccatac aagagaagat ttaaaaaatt tttctaaagt atttgatgaa
    17401 tatgattata gaaaacaact gatagtagat attttaagta gtgagaatca tagtataaat
    17461 tcttttgacg cagtagttgg tcgtggtgga cttatgaagg ctataaaagg tggtacctat
    17521 actgtgagtg aagaaatgat tgaggatatg agaaatgaaa taaatgggga acatgcatca
    17581 aatttaggtg cattattagc taaaactata gctgatgaaa taggagtaca atcatttgta
    17641 gtagacccag tttctgtaga tgaatttgat gatgtatcga ggattacagg aatatcagac
    17701 atagaaaaag caagttggtt acatgcatta aaccataaag ctgtttcaag aaaaattgca
    17761 caaaaactag ggggaaaata cgaggattat aactttattg tagctcattt aggttcagga
    17821 atatcgattg tagcacataa acaaggcaaa atgattgatg gaagtggtgg taggtctgat
    17881 ggtccatttt ctccagaaag gagtggtgga ttacttgcat atcctcttat agaattgtgt
    17941 tactccggaa aatatactca tgatgaaatg gtcgcaaaag taagttcgat tgggggaatg
    18001 tacgattatt taggaacaaa ggacatgata gaaattgaaa acagaatcaa ggagggtgat
    18061 gaaaaggcaa aacttataat ggatgcattt atttatcaag tagcaaaaga aatttctatg
    18121 tatggagctt cactttcagg gaatgtagat agagttatct tgacaggagg gatatctcac
    18181 tcagaaatgg tagtcaaagg agtagcagaa aaagtttcat atctagctcc aatagagata
    18241 gtaccaggag agatggaaat ggaggcactt gctctagggg cacttagagt acttaatgga
    18301 gaagaaatag caaaaactta ttaatattta ggagaaagag tatgttaaaa gattttaata
    18361 atttattaga agaggctaaa aagcttgaac ctgttattgt aagtgttgct gttgcagaag
    18421 acaaagaagt attaaaggct gtgaaatcag caacagattt aaacattata aagccaattc
    18481 ttgtaggaga caaggataaa atagaaagta tatcaagaga aattggatta aaaggtcatg
    18541 aaataatagg ttgtcaaaat cccgatgaat cagttaaaaa agctgtagag atagttaaaa
    18601 ataaggaagc taaagttctt atgaagggat tagtaaatac aagtgtctat atgagagcta
    18661 tattaaataa agaaacgggt cttagaacag gaagattatt aagtttgctt gcagtatatg
    18721 aattacctca atatcataaa cttctttatt gtactgatag tggaataaat gtatcgccaa
    18781 acttaaatca aaaaaaagat ataatgacaa atgcacttct tactatgaaa agtataggca
    18841 tggaaaatcc taaaactgcg atacttactg caaatgaaat ggtagaccct aagatacaat
    18901 ctacagtgga tgccaaccta cttgtagaga tggaaaaatc gggagaaata ccaaagtgta
    18961 tagtggaagg tccaatagca tttgatgtag catttgatgc acatgcagca gaacataaag
    19021 gtattaaaag taatattgct ggtgatgttg atttattagt atttccaaat atagaagcag
    19081 ggaatatatt aggaaagtca tggcttagat ttaataaagc taagtgggca ggtatagttt
    19141 taggagcaac aaatccgata attttaggtt cacgttctga cacagctgaa ataaaaataa
    19201 attcaatagc attagcgtgc ctagcatcac aataagaaat tattattaag gaggaaaaag
    19261 tattatgaaa tatgcaaaaa gaatggatat ggtaaaagct tcagctataa gagattctca
    19321 aaagaaaata gctcagaagg tagcaagtgg tggaactgtt atatcttttg ctgcagggct
    19381 tcctgaccca aatttatttc caatagaaga gataagtgaa ataactcaaa aagtacttaa
    19441 tgaaagggga aaatttgccc tagcatatgg acctactaaa ggtgaagatg agttgttaga
    19501 tctattagta aaaagaatga aagaagaaga aaatattgat acaaaagcag aaaatattat
    19561 aataactact gggtcacaac aagggatagc attaagtgct atgattttat tagaaaaggg
    19621 agatgtagta gttacagaaa atccaagtta cttaggtgca ataaatgcat ttagacctta
    19681 tgaatgtgat tttttaggag tagatacaga tgaagaagga atggtaacag atgatttaga
    19741 caaattatta tctgaaaatt ctaacataaa ggtgatatat gtaataccaa catttcaaaa
    19801 ccctacagga aaaacatggt ctttacaaag aagaaaagat tttatggaag tcgtgaataa
    19861 atatgatgtc atagtaatag aggataatcc ttatggagaa ataagattta caccagaacc
    19921 tctacctaca cttaaatctc ttgatactaa agataaagta ttgtaccttg ggtcattttc
    19981 aaaggtatta tgtccaggat ttagagttgc ttggatgtgt ggtaatgtag acatcataga
    20041 taaggcagaa cttttaaaac aaggtataga tttacagtca aatcaatttt cgcaagtaca
    20101 agtaatagaa tttttaaaga aatacaactt aaatgaacat atagaaaaaa taagagcaga
    20161 gtataagaaa agatgtctgc taatgttaga tgctatgaag aaacatttcc cagaggaagc
    20221 taaatataca gaaccagatg gaggaatgtt tatatgggtt gaacttcctg aaagtataaa
    20281 tgtagatgat ttattagata aagccattga tgctggagtt gcttatgttt ctggagagtc
    20341 cttctttgca aataatggtc caaaaaatac tatgagactt aactatacaa caatgtcaga
    20401 agaacaaatc gttaaaggtg ttgagatact tgcagaagtt ataaaaaaag aactcaatta
    20461 tgcttgtgaa taaatataaa aagtgagggt ttaaatatgg gagaaaaaga aaacggtata
    20521 aagtttggaa agttaatgtt tcaacttgtt attatagcaa tatcttggga attagtttat
    20581 attataccat ttatccaata tacattatat gatccaatcc taaaagcatt agaatgtaca
    20641 aacactcaac taggttttct acttacaatt tatgggttag gtaatatatt tggagctcct
    20701 ataggaggct ggttagcaga tagatttgat tatagaaaga taattttagg ttctgtattt
    20761 cttaatggag tagtatcatt tttatttgca tttaatatga actatccatt tgcagttgct
    20821 acttgggtag gttgtgcaat cacatcatta gttatgaact atccttctat ggtaaaaata
    20881 cttagagtta taggaaaaga taatcaaggt aaggtttatg gatttaatga agctatggtt
    20941 ggagtttctg gagtagtaat gggagcaata ttcttatata tttatacatg ctttgcaact
    21001 cctacattgg gtatgagatg ggtaatgata tctttaggaa tattatcaat agcaatgtgt
    21061 ccagtattat ggtttgtaat taaagatgta gatattaaag aagaaagaga agaagcaggt
    21121 gaaaaaatgt ctgctggtga ttttataaca gtactaaaat caccaaatac ttggctagta
    21181 ggaataagta tattctgtgt atattcattc acagttacaa tgtcttattt tacaccatat
    21241 ataacatctg tacttggagg ttctgtagca ttatctggtg cattagcaat aataagacag
    21301 catggtctaa aattatttgg agcaccattt ggaggatatt gtgcagacaa attaaaatct
    21361 cctacaaaag tacttttacc aatatatgta tttggtatag cagtaatagt tttattccta
    21421 gtattacctg catcaactcc aatgactata ttcatagctt taacatttgt agtaggtata
    21481 ttaggttaca tgggaaaagg tatatattat gcagtacaag atgaagtaaa agttccagtt
    21541 aaatattcag caactactat aggtatagct gcagctttag gattttcacc agatgtattc
    21601 caatttgcat taataggaca ctggatagat acttatggca ataaaggtta cacatataca
    21661 tttatattcc aaatagcaat tctagttatt ggaatactat cttgtttata tatattaaag
    21721 gttaaaaaga gaagagcaag taaattagaa gcttaaatat tcaatttggt aattaagtat
//
LOCUS       DAELTA010000056        21574 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.056, whole genome shotgun sequence.
ACCESSION   DAELTA010000056 DAELTA010000000
VERSION     DAELTA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.056"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            329..718
                     /locus_tag="KPG13_001772"
     CDS             329..718
                     /locus_tag="KPG13_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0985505.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            1038..1547
                     /gene="vanZ1"
                     /locus_tag="KPG13_001773"
     CDS             1038..1547
                     /gene="vanZ1"
                     /locus_tag="KPG13_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG0985506.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1649..1843)
                     /locus_tag="KPG13_001774"
     CDS             complement(1649..1843)
                     /locus_tag="KPG13_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG0985507.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(2359..2751)
                     /locus_tag="KPG13_001775"
     CDS             complement(2359..2751)
                     /locus_tag="KPG13_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0985508.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            3318..3680
                     /locus_tag="KPG13_001776"
     CDS             3318..3680
                     /locus_tag="KPG13_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985509.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            3664..4389
                     /locus_tag="KPG13_001777"
     CDS             3664..4389
                     /locus_tag="KPG13_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985510.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            4409..4828
                     /locus_tag="KPG13_001778"
     CDS             4409..4828
                     /locus_tag="KPG13_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985511.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            4818..5342
                     /locus_tag="KPG13_001779"
     CDS             4818..5342
                     /locus_tag="KPG13_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985512.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            5654..6211
                     /gene="efp"
                     /locus_tag="KPG13_001780"
     CDS             5654..6211
                     /gene="efp"
                     /locus_tag="KPG13_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG0985513.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            6303..6758
                     /locus_tag="KPG13_001781"
     CDS             6303..6758
                     /locus_tag="KPG13_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG0985514.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            6921..7631
                     /locus_tag="KPG13_001782"
     CDS             6921..7631
                     /locus_tag="KPG13_001782"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG0985515.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            7685..8755
                     /locus_tag="KPG13_001783"
     CDS             7685..8755
                     /locus_tag="KPG13_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985516.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            8783..12337
                     /gene="smc"
                     /locus_tag="KPG13_001784"
     CDS             8783..12337
                     /gene="smc"
                     /locus_tag="KPG13_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG0985517.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            12364..13644
                     /gene="ftsY"
                     /locus_tag="KPG13_001785"
     CDS             12364..13644
                     /gene="ftsY"
                     /locus_tag="KPG13_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG0985518.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            13747..14115
                     /locus_tag="KPG13_001786"
     CDS             13747..14115
                     /locus_tag="KPG13_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG0985519.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            14112..15464
                     /gene="ffh"
                     /locus_tag="KPG13_001787"
     CDS             14112..15464
                     /gene="ffh"
                     /locus_tag="KPG13_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG0985520.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            15502..15774
                     /gene="rpsP"
                     /locus_tag="KPG13_001788"
     CDS             15502..15774
                     /gene="rpsP"
                     /locus_tag="KPG13_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG0985521.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            15839..16066
                     /locus_tag="KPG13_001789"
     CDS             15839..16066
                     /locus_tag="KPG13_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG0985522.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            16174..16689
                     /gene="rimM"
                     /locus_tag="KPG13_001790"
     CDS             16174..16689
                     /gene="rimM"
                     /locus_tag="KPG13_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG0985523.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            16698..17393
                     /gene="trmD"
                     /locus_tag="KPG13_001791"
     CDS             16698..17393
                     /gene="trmD"
                     /locus_tag="KPG13_001791"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG0985524.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            17578..17928
                     /gene="rplS"
                     /locus_tag="KPG13_001792"
     CDS             17578..17928
                     /gene="rplS"
                     /locus_tag="KPG13_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG0985525.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            18249..19145
                     /gene="ylqF"
                     /locus_tag="KPG13_001793"
     CDS             18249..19145
                     /gene="ylqF"
                     /locus_tag="KPG13_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG0985526.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            19534..20826
                     /gene="brnQ"
                     /locus_tag="KPG13_001794"
     CDS             19534..20826
                     /gene="brnQ"
                     /locus_tag="KPG13_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0985527.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         9109 a         1993 c         3834 g         6638 t
ORIGIN      
        1 taatacatat aataaaactt aatataaatt aagtatatga aataaatatc ctgtatttaa
       61 gcttatagat tataatgaaa tataatacat gttgcaataa taaaacgaac taaaattatc
      121 acactaaagc ataaaacata ctctaatcat gttagataaa tttttgaata tataatccaa
      181 aagtactctc taaagtagag tacttctttt ttattaatta tttttatata gaataaattg
      241 gaatttatat ttattccaat ctaattgatt acatacactt attgttataa agtttagtta
      301 tacataaatt ggtaagaggt gtaaactaat gaagattaat aaattaccac atgcagaact
      361 aaaactaatg aagtatattt ggggagtaga tgatgtatta gcctcaagag atatcataga
      421 agacatgaag ttgaaatacg attggaaaaa gagcacaaca ttgacatttt taaaaaattt
      481 agtagataag ggctttttaa ctactgataa ggtagataga tgtacgcatt atactatagc
      541 tataaaagag aaagattact taaaagttga gacaaagagt ttttttagtt ttatgcataa
      601 caattcattt aagagtttta tttcagcact acatgatgat gaagttttag atagtaagag
      661 cttggacaaa ttagaagaat actttaaaaa tttgaaagaa gaagatattg atgactaata
      721 aagctatcag tatcttttta gtttgagaca attgagagta gcgtttggat ttaatgttag
      781 ttttatgtga tggtagaaca ttatataaag aaaagtgatt atttccttag tgtttgatag
      841 taaaaaatat agagaataat accaattaaa atttaaagat atatgaaata ctacaatagc
      901 ttaaatcatg taaatatgat aaagatattg aaattataaa aggaatataa ttttagaaga
      961 aatagttgac agataaatta ccttactata ttattgtatt aataaaataa tacaataata
     1021 tagtaaggag gtaataaatg aaatctagaa aacataatat tacaaaagga ttatttatag
     1081 tttacataat tattttaaca tggataatac tatttaagtt gcaatttgat atctcatctc
     1141 ttgaaaccat gaatcttaga agcataaact taataccttt tgctgggtca cttataatta
     1201 ataatagagt agatatatca gagattatat taaatgtggc tatatttgta ccatttggga
     1261 tatatgtgtg tatgctaaaa gaagaatgga gctttataaa aaaagttata cctattttta
     1321 taactagttt ggtatttgaa acacttcaat atatatttgc actaggtgca agtgatataa
     1381 cggatttgat aggaaacaca ctaggaggaa tcataggaat agctgttttt atgctattat
     1441 cgaaaatatt taagaataat accataaaaa ttatcaatgt attggcattg attgtgacaa
     1501 ttattgttgt attattttta ggattagtta tttttgcaaa cttatagaaa taagtatata
     1561 tgtttagctt aaaatagaac gtaagataca gttacatata aaattattta cacataaaaa
     1621 gcacctatac aggtgctttc ctaaattatt aaagaattat tgtatcttga aactcttgta
     1681 tgttcaattc ttgattttct cttgtttttt ctaataaatc taacgcacaa tttatacaaa
     1741 ctttaatttc tgcattggta tttttctcat aataagttat atatttattt ctatatttat
     1801 ctatttcttt tccacaacga ctacacatta caattacgct catatcttag ttcctccatc
     1861 tctaattatt cttaatatat tcaatagtat cacttttctg aaaataatga ttgttaaaac
     1921 tgtatcaaat ttagatttac tttttattat aatgcatttt aataaaaaaa taaaggttat
     1981 gggtaaaata tttatagaaa aaagtatatt attgaataga ttataaaatt ttattatttt
     2041 ttagcagtgt taaatacaca ttaatatcta taaagtgaca aaataaaaaa tactatttaa
     2101 atagatatta aaaaattgat accaagtatt tatttgctat atgaataaca atttatttag
     2161 gaactttact ttaacactgt ataataataa agtaattgct aaaaacgttt gatgatttgg
     2221 atgtattttt agaaattaga aaaatatata cttactgact aatgataatc ttatatgtat
     2281 tttagtttga gtgatttaag aaaagtgttt tgaatgggga ttattttata gagtaaataa
     2341 atatataata gtagaaaatt acatataaag agtagtgttt atttcattac cttctttatc
     2401 tgttccgata gggtcttgta gagatacttg agttttattt tttttgttag atctaatatt
     2461 cattaaaatc tcattttcaa tacatctaga agcatatgtt gctaatcgag ttcctttatc
     2521 tatgttataa gtttctatag ctttaattaa tcctattgta cctatggata ttaaatcatc
     2581 ctgatcttcc gttgaattat tatatttctt tgctatataa gctactaatc tcatgtttct
     2641 ttcaattaat gtatcttttg cacttttatc gttttctatt ttaaatttag ttaaatactc
     2701 aatctcttct tcaggggtta agggtttttc aaaagattta agagctgcca ataggtcacc
     2761 cccttttcaa cggtcacttt aaattatatg aaggagactt atccaagttg actgttatct
     2821 aataaaataa tatgcatatt tttagaattt aaaacatata tttgcaaact tttgaatgga
     2881 atatgacata aagagtggga atttaaattt aagtttttgt tgtatatata tgaaataagg
     2941 cgaagaaatt attgatttat aaagtataaa ttggaattga ggtgtcaata aatataaaag
     3001 gaaatagtat ttatatatgt tttggtgttg aagtaaaaga ttaatgcaaa gcattagagt
     3061 ctataaaaat atgaatatag gaggtgtaat tttacaatac ttaaaaaagt aaatgtaatt
     3121 aattgtttgt ataaagttga ttacaagcaa aagtatcagt catagttaat actgttaagg
     3181 gtgaaggtgg tgtgttaatt tatatatgta tgggaagaaa ttttgattac aatgaaaaaa
     3241 cttacaaaag atatttatga aaaaacttat agttaaatgt ttatatataa aatattattt
     3301 aggggttgtt tttattattg aaaataagaa agagtggttt tatatcaatt gaatgtataa
     3361 taagtattgc tatattatat gtggctgttt atttagtttc tacatcattg tataattgtt
     3421 atagttttat cagtagaaat atatctgaca gagaaatgtt aagtacagca aaaaaatata
     3481 tagaagatga gaagtataga atacaaaata gtaagtatga gttaattgaa gataagatag
     3541 aaaaaaatta cataaatgga tatgaaatta acagtaaaat agagcaaatt ttagattatt
     3601 atcaatgcta tgaaataaat atagagataa aaaatgaatt taaaaaactg aggtttaata
     3661 gctatgttac tagaaaataa aggctctata ttaataataa cattgataat attttctatt
     3721 ataagtactg tgtgcattat gtgtataggt ttaatttata cacataataa tatgtttgag
     3781 ttaggataca aggacattca actaaaagaa atttctttaa gtggaataga aatatcagta
     3841 agtaatgtac tttcttgtgt tgaggaagct attgatgtat ctaataatga agacgaattt
     3901 agagaatatt tcttaggaaa taatatgatt gaatgtataa aaaaaataaa agatacatct
     3961 tatagtagtt tagaagaact ttctttaaaa gtagaaaata atactattta tgacagagga
     4021 gatttttttg aatttgatat agaaagtaat gttagagaaa aagaattttt aaaagcaata
     4081 aaagtaagag tgaaaatcaa aaatccatgg ataggaattg acattgaaga ttatatggat
     4141 aaatctaata acaataaaat tgatgacaaa acattcaata attataaagc atctgaaaat
     4201 aatgaaattc taaatcaatt taaaaatgaa agtgaaaata taaggaatca agaggttggt
     4261 ggatttacag aagaagatga agaagatgaa gaatatgaag aagaacttaa acaaaacata
     4321 attgaaaata taaagaagaa ttttgatgaa aaaaacctag tacaggtata taagtatagg
     4381 ggtgtataac tttgaaaaaa gaaggaatgt gactgtaata gaattaatag tatctatcag
     4441 tataatactc ttgatagtta cattatcttt tccaaaagat aatcttgaaa atcataaaat
     4501 aaatttattt gcacgtcaac tttgttcaga tataaggtat gttaggcgaa ttaatatgtt
     4561 tagcaactac aatacatata tatattatat aaatcaaggt aaacacgaag gttatgtatt
     4621 gagaaaagat ggtaaaaaca ttaaatcaat aatgctacct aaaaatgcaa aaatagctag
     4681 gagtattgaa ataataaagt ttaaatctga tgggtcgcct caaaaggcgt ctacaataga
     4741 gatatataat aatagattaa gaaaaactat aacaataaca cctgtaagtg gaagagtact
     4801 tctgaaagag ggaaaatatg aaacataaat atggttactt gcttttggaa agtgtcgtat
     4861 cattgtcttc aatggtgata attatactag ttttatattc tatatttttg tctacaataa
     4921 acttaaagtt aaaagtagaa gataagatag aactacaaca acaaagtctt gaaatcataa
     4981 aatctatgga aggaattatt agcaattcta tgggaataat gaatgtatct aattatgaag
     5041 aaacatttaa aaagacaaca tctataaaat gtagatattt agatgaaaat aataatgaag
     5101 aaggcataag taataaagaa attatattga atgaaagacg aaataagtta tttgtaaatt
     5161 ctttaaatgg cgaaagtagt caagcaggtg gttatgaaat tggagattat gtagatgaga
     5221 tgtatgtatc aattactaat aatggtcaat atgttaatat taaattgaaa ttatcaaaac
     5281 gtagtcaaaa atatgagact gatttcaaaa taaaagtatg gaattttagt gaaagtatat
     5341 agaaatacat aaatttaaga gaaagaagtg acatcaataa gtcatataaa atataattat
     5401 tgagaactat ataactaaat aatatcaata tgtaaagtgt atagattaag aatatagagg
     5461 ataataataa aaatatagaa taagtaattt gccaaatact tgtttatatt gtataactta
     5521 tgatattatg atattagtaa aaatttgtaa tcctgacttg acagaattca agtaagagat
     5581 ataattttaa aggcatagta atgtaaaatt tactaaaaaa taagaaattt attttaatat
     5641 ggaggaacaa aaaatggtat cagcaggtga ttttagaaaa ggtgttacat ttgaaaaaga
     5701 tggacaacca tgtttagtag ttgattttca acacgttaag ccaggtaaag gagctgcttt
     5761 cgttagaact aaatacaaaa acttaaaaac aggagctata agagaagaaa gtttcaatcc
     5821 aagtgaaaaa ttccctaaag ctgttataga tacaagacaa atgcaatatc tatacaatga
     5881 tggtgagtta tattatttta tggatcaaga aaattttgaa caaataccat taaactatga
     5941 gcaagttgaa gatgcaatta agttcttaaa agaaaatgaa gttgctacaa taagatttta
     6001 ccaaggacaa ccattccaag tggaagcgcc aaactttgca gagctagaag ttacagatac
     6061 agagccaggt ataaaaggtg atacagcaag taatgtaact aaagcagcta cagttgaaac
     6121 tggagcagtt gttcaagttc cattatttat aaatactgga gataaagtaa agatagatac
     6181 tagaacaggt gaatatttat caagagtata aaaatttatg tatataaaag cttatatgtc
     6241 ttaataatat aagatatatg agcttttagt ttatatataa aaaagaaagc gaggggtgtt
     6301 acatgaaaca tttatatgaa gaagttgcat acttaaaagg cctagcagaa ggactagaaa
     6361 taagtgctga aagtaaagaa gggaaaatga tacataaaat agttgatgca ttagaagtat
     6421 ttgcagatgc tattgtaaca ttggatgaag aacaagaaga acttcaagat tttgttgaat
     6481 ctatagatga agatttagca gatttagaag aagatattgc tgacatggaa gaagatcttt
     6541 atgaagaaga tgacgatgag gatgatgagg acttcagcta tatagaaatg gaatgcccaa
     6601 attgtggaga gttagtagaa atagacgaag atttgttata tgatgatgaa gtagatgttg
     6661 tttgtcctga ttgtaaggct gtgatattat catcagaaga tgattgtgat gatgattgta
     6721 catgtggtgg ttgttcaagt tgtgatgata gagagtaaaa attcataaga attaaaactg
     6781 cctatgatgc ctatgagata ttttgcttca taggtagttt tatttttatg aggaagggta
     6841 ttatgtattt tggttttgca atacttggac aaaaatagta cactagaatt tatagaatag
     6901 gattttggag ggatgtaaat atgaaaataa gtaaaaaatt actagataat atacaaagat
     6961 ttgaaaatgt tataaattat aaatttaaaa ataaagaata tatactagaa gctcttactc
     7021 atagttcata ttctaatgaa aataaaaaat ataattttaa cgaaagattg gaatttttag
     7081 gggattcggt tttaggcata gtgataagtg attatttatt taatgaagaa gctaatctgc
     7141 ccgaagggga actaactaaa cttagagcaa atatagtttg tgaagattca ttaagtgaag
     7201 tagctaatga tataaattta ggtattcaca tgttattagg tagaggagaa gaagcaacag
     7261 gaggaagaca taggacttca atattagcag acgcatttga agctgtaata gctgctatat
     7321 atcttgatgg aggttttgaa agtgcaagac agtttatact tcatcatatg gaaaatataa
     7381 tttatgattc aagaaaagga aatatattta gagactacaa aacgcatctt caagaggtgt
     7441 tacaagggaa tggagaaaat aatatctggt acaggcttat agaagaaaaa ggaccagacc
     7501 ataataaaag atttgtaatg gaagttggaa ttaatgacga tgtacttggt ataggagaag
     7561 gcaagagtaa aaaagaagct gaacagttag ctgctaaaat agctttaaag aaaaaattat
     7621 gggaaaaata aatataatat gtaaaaatat aaaaagaaat atttatatta gaaggatgtt
     7681 gtagatgaaa aagagaatta tacctatatt tgttcctcac aaaggatgtc ctcatgattg
     7741 catattttgt aatcaaaaaa aaataacagg tgtgagtact gatgttacta gtgaagatgc
     7801 aagaaatata attgaggaat gtttagagac tatagataaa gatgcagatg tagaaatagc
     7861 attttttggt ggtagcttta ctgctataga tattgatata caaaaaagtt tattatcagt
     7921 tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt agaatgtcta caagaccaga
     7981 ttgtattgat gaagatatat taagtatgtt aaaagaatat aagacaagta taattgaact
     8041 aggagtacaa tccttagatg aaaaagtatt attagatagt ataagaggtc atcaatctga
     8101 aattgtatac aagagttcaa agatgataaa aaatagtgga atcaaacttg gattacagat
     8161 gatgattggt cttccagctg acacagaaga aaagtgtata tttacagcaa aaaaatttgt
     8221 agagctaaaa ccagattgtg ttagagtcta tccaactttg gtagtaaaag atacaggtct
     8281 tgaaaagtta atggaacaaa ataaatataa tccatttact cttgaagaga gtgtaaatat
     8341 agtaaaaaaa gttcttgtac tgttttatgt taataatatc aatgtaataa gagttgggct
     8401 tcaagctact gatgatatac aaattggaaa agctgtttta gcaggaccat atcatcctgc
     8461 atttagagag ttggtagaag cagatatgat aaaagattat ttagaatttg ttattttgca
     8521 aaataaaaat ataaagcaaa tgttggtcaa atctaataaa aagaatattt ctaaaatcat
     8581 aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa tttggtgtgc ttttaaaaac
     8641 acaagaatca gatttagata taaatcaatt agaaattgtg ttagatggga aaagtttaat
     8701 tagtgctaat atgagagaca ttcatagaaa attgtatgac atttatgacc tttagtttta
     8761 ataatgaagg gagagaaaag atttgtattt aaaaagacta gagttaaagg gatttaaatc
     8821 ctttccagta aaaacagata ttatttttaa agaaggaata acagccatag taggaccaaa
     8881 tgggagtgga aaaagtaata tatctgatgc tgtaagatgg gtattaggag aacagagtat
     8941 taagagtctt agaggtgata aactcgaaga tgtaatattt gcaggtacag atacaaaaaa
     9001 acctatgaat tattgtgaag tggctcttac tattgataat tctgaaaatc aattggaatt
     9061 agattttaca gaagtaacta taagaagaag ggcttataga aatggtgaaa gtgaattttt
     9121 tctaaataat aaaagttgta gattaaaaga tataaaagaa gtctttttag atactggtat
     9181 tggaaaagat ggttattcaa tcatagaaca aggaaaagta gatgaaatat taagtaataa
     9241 tcctttaagc agaagaaaag tttttgatga agcttgtggc atatcaaaat atagatataa
     9301 aaaacaggaa gcagaaagga atttaagtaa tacaaaggaa aatttagaaa gaatagatga
     9361 tgtatatata gaaattgaaa atcaattaaa gcctcttttt aatcaacaga caaaggcaaa
     9421 aaagtacttg gaaataagtg aaaaattaaa gacacttgaa gtaaatagct ttataagaga
     9481 aattgaagga atagaaaaag aattaagtga ggttaatgaa catcgtaagg ttattgaaaa
     9541 ggagctcaat gaaaaagaag aacaaaaaaa tgttgtagag aaaaagcaag aagatattaa
     9601 taaagaggta gaggtattac aagatgtaat tgaaaaatct gtagattata taaattcaat
     9661 taaaggagtt atatcaaaaa aagagtccca aataaattta attaaggaaa gaataagaaa
     9721 ttttacgaat gaaattagta gaaaaaattt agaaataaag gatataaaag agaagctaaa
     9781 tgaaaataag caatacataa aagaattaga aagcaataaa ttatcaggta gtgaagaact
     9841 atctaccttg caggaaaata taaaagtatt ggaaggaagt aaagataaac aaaaaattaa
     9901 gttagaatcc ctaaataatg agatagaatt attgaaagaa agtataattg atatattaaa
     9961 taagaaacaa gagtttagta ataagctgtc tactttaaac gcaaataaag aaaatatgaa
    10021 tatcagagat gaaaatataa attcagagat aacagagtta aacaaaaata ttgaaataaa
    10081 atcatctgaa ttagatacta taaataaaga atttaatatg caaaatgaaa atttaaagaa
    10141 tgtaaataat agacataaag aattatcaat taatttgcag gattcaatta gtgaacacaa
    10201 taaactagaa gatgaaatac agaaaagtaa atataattta aatggatata attcaaagtt
    10261 aaatgtctat atagatatgg aaaatcatta tgaaggattt aatagaggag taaaagaagt
    10321 actaaaaaat aaaaatttaa aaggagttca tggagcatta ggacagatta taaatgtacc
    10381 tgaaaagtat gagaaatcta tagaagcagc tcttggtgct tatatgcaaa atataataac
    10441 tgataatgag tttagtgcta agtttgcaat aaactatcta aaacagaata atttaggtag
    10501 agtaacattt cttccattaa atataattaa atcaaataaa ataagtctag gaaatttaaa
    10561 agcaaatact aagttcatag gaatagcaag tgatttaatt acatttgatg aaaagtatag
    10621 aaatataatt gaaaatatac ttggaagaac aatacttata aataatatag atgagggcat
    10681 aaaatttgca aaagaaactg gtcataggtt taaaatagta actttagatg gtgaaatctt
    10741 aaatccagga ggttctttga ctgggggaag tctaaaaact aatgggaata tactatcgag
    10801 aaaaagatac attaatgaat atacagaaaa gataagtaat atcaagaatg aaatttcaca
    10861 tctagaatta aaaagagaat ctttagacaa agatgtgaaa aatatcaaaa atgaaattga
    10921 ttcacatgaa agtaaaataa aagatttaga aaaaagtata ataataaaaa gtacaagtat
    10981 aaagaatgtt gagtctgaaa ttgaatcgtt aaaaggtagt attactaaat tagaaaatga
    11041 gaagaatgat ttaaattcta acctaaatta tacacttgaa aaaagtgatg atgttagaaa
    11101 agatatggaa gagttagatg atttatacaa taaaaataaa gaaaaaattg atgcattgaa
    11161 tgaggaaata aaaagataca atgatttata tgataaagaa aaatctgaat ttgatgaatt
    11221 gaacttaagt ttagtgaaga aaacagaagt ttacaatagt atagttagag atataaaaag
    11281 aatatctggt gaaaattgtg agctagaaga aaaaaataaa caattagaag agtcattaaa
    11341 ttatgaagaa catgaaataa ttaaattaca agattctata ctcactgaag aaaaagaaaa
    11401 agaaaattta acaaaacaac taggggatag taatagaaat cttgaaacta gaaaaatagc
    11461 aaaagatgat ttaaaaaata gttttgatga aattaataaa gaattaaaaa ctatagatag
    11521 acagcatata gaacttaaag aaagtttatt taaagttggt ggaagattag aaagattaaa
    11581 aactagtcaa gacacatata taaataagtt gtttgagcaa tatgatatga ccttagtaca
    11641 agcattggaa attaaagatg aagatttgga tatagataga aaattcttag aaagtctaaa
    11701 gagagaaata agaagtcttg gaaatataaa tatagactct ataaaggaat atgaggaaat
    11761 aaaagaaaga tacgattttt atagcgaaca aaagcaagat ttagaagagt ctatggaaga
    11821 gatagaaaaa cttatacata ctttagaaga aaatatgaaa tctgaatttg aaattaagtt
    11881 tgaagaaata agtaaaaatt ttaaatatgt atataagaga ttatttggtg gaggttgtgg
    11941 agaattaact attttagata aagaaaattt attagaaagt gatatactaa taacagctca
    12001 acccccaggt aaaaaaatga aaaatttaaa cctattatct ggtggagaga aagctttaac
    12061 ggcaataagt atattatttg ctatattgat tacaaagcca acaccatttt gtatattaga
    12121 tgagattgaa gcgccacttg atgacgcaaa tatatttaga tttggagaat ttttgaagga
    12181 tttatctaaa gagactcaat ttatatcagt aactcatagg agaggaacaa tggaagctgc
    12241 tgattatatc tacggagtta caatgcaaga aaaagccatt tcaaaagtta ttagtttaaa
    12301 attaaaagaa gctcaggaaa taacagatat tatataactt agattgacat aggaggaata
    12361 acagtgttaa aaaaattatt tggttttgga aaagacaagg aaaaggaaat agaaaaaaaa
    12421 gatgctgaag aagaaataga agttgaagac agtgttgata atttagaaaa cttggaagaa
    12481 actatttttt caggacttga agaagaagtg atagataaag tggaagatat tgaagaaaaa
    12541 aatgaggaaa gtgtcaataa tgaaggcact gaagaaatcg aaaactttga aaaaatagaa
    12601 gttgagatgg atagtaatgg aaaagaagta gaaaatattt caaataatga ttctcaagaa
    12661 gagataatcg aagaattaga aaattatgat gaaattgagt ctaaagagat agaagataaa
    12721 gaagataaga aagttaactt gtttgaaaga ctaaaacaag gattgacaaa agcaaagcaa
    12781 ggaataactg atagaataga tgaagtttta aaatcatata caaaaataga tgaagaatta
    12841 cttgaagatt tagaggaaat tttaataaca gcagatgttg gtgtaaatac aactatggat
    12901 ataattgaaa gactaagaga taaaataaag caaaaaggta ttactgaacc aataaaggta
    12961 agagaagaac ttaaatctat agttgaagat attcttacaa atgaaaactc tacactagat
    13021 atagaaccag caccatgtat aatattaatg gtgggtgtaa atggggtcgg taagactact
    13081 acaataggta aactagcaaa tagatacaaa aaagatggta aaaaagtttt attagcagca
    13141 gcagatacat ttagagcagc agcgacagag caattagaaa tttgggcaaa tagaaccaat
    13201 gtagacataa taaaacatca agagggagca gaccctggag ctgtagtatt tgatgctata
    13261 aaagctgcaa aagctagaaa aacagatgta ttaatatgtg atacagcagg aagattacac
    13321 aataaggcta atcttatgaa tgaacttgga aaagtattta aaatagtaga tagagaattt
    13381 ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta caacaggtca aaatgcagtt
    13441 gttcaagcaa agacatttaa agaagttgca gatataacag gtatagtact tacaaaactt
    13501 gatggaactg caaaaggtgg agtagtcctt gcagtaaaat cagaagtaga tgtacctgta
    13561 aaacttattg gagttggaga aagtgtagaa gacctacaag atttcaatgc aaaatcattt
    13621 tcagatgcat tatttggtaa ctaatgttct tttaaaaata atggttgaca agagtgttgt
    13681 tatgatataa aatacaatag tgtaaagtaa ataaccttta cagtatactg ctaggaagtg
    13741 taagatatga atatagaaaa aatggtcgaa attggattgt tatttgaaca gtataaagaa
    13801 ttattaactg acaagcagaa agaaatagtt gctctatact atgaagaaga ttattcttta
    13861 ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg tttatgatac cttaaagcgt
    13921 tcagaaaaaa tactaagaga ttatgaagaa aagttacact tagtatcaaa gattcaagag
    13981 caggaaaaaa atattaagat tataaaagat aaaattattg acattaaaga agatttattg
    14041 cacaatagag attgtgctaa tttaatccct aagctagaaa atatagaaga tgtatgtagg
    14101 gagatgataa aatgatattt gaaggattgt ctgataaact acaaggggca tttagtaagt
    14161 taaaatctaa aggaaaactt acagaagtag atgtaaaaaa tgctatgaga gaagttaaac
    14221 ttgctcttct tgaggcagat gttaacttta aagtagttaa agatttcata aaaaaagttc
    14281 aagagagatg tgttgggcaa gaagttatgc aaagcttaac tccagctcag catgtaatca
    14341 aaattgtaaa tgaagagctt acaagtttaa tgggagatgt acaaagtaaa gtaatgattt
    14401 cctcaaagcc tcctacaatt ttaatgatgg ttggattgca aggtgcaggt aagactacta
    14461 cagcaggaaa acttggagga tattttaaaa agcaaggtaa aaaacctcta ttagtagctt
    14521 gtgatattta tagaccagca gctataaagc aacttcaagt tgttggagaa aaattggata
    14581 tacctgtatt caatatggga gataaagaaa atccaataaa tattgcgaaa gcaggtttaa
    14641 gccatgcgat taaaaatgct aatgatttag ttattataga tacagctggt agacttcata
    14701 tagatgaagt acttatggat gagttaaagt ctattaaatc agaagttaag ccacatgaga
    14761 tacttttggt tgtagattct atgacaggtc aagatgctgt aaatgttgca gaaagcttca
    14821 atgaggcact tggtgttgat ggagtagtat taactaaatt ggatggagat actagaggtg
    14881 gtgctgcgct ttcaataaga gcagttacac aaaagcctat aaaatttatg gggatgggag
    14941 aaaagctaga tgatatagaa cctttccacc cagatagaat ggcatcaaga atattaggta
    15001 tgggagatgt cttaagttta atagaaaaag cacaagaaaa tatagactta gagaaagcta
    15061 aagaattaga gcaaaaaatt aaaaaacaag agcttgattt tgaagatttc ttacaacaaa
    15121 tggaacaaat acaaaatatg ggacctcttg acaagatact tggtatgata cctggtatgg
    15181 gaaatgtaaa agaccaactt ggagatgtag atttgaatgg caaagaaatg aagagaacaa
    15241 aagccataat tcagtcaatg actacatatg agagaagaaa cccaggagta ttaaatgctt
    15301 caagaaagaa aagaatagct aaaggcagtg gaactagtgt acaagatgta aatagactta
    15361 taaaacaact taatgaaatg aaaaaaatga tgaagatgtt tgctggttca caaaagagta
    15421 tgaagaaaag gggcggctta ggcggtttac ctttttttaa ataaaatata aaaaagtaaa
    15481 aattattcgg aggtgactgt aatggcagtt aaaataagat taaaaagaat gggagctaac
    15541 aaaaaacctt tctacagaat agtagtagct gattcaagag ctccaagaga tggaaaattt
    15601 atagaagaaa taggatacta taatccaatt tctgaaccta aacaagtaag aataaatgat
    15661 gaaaaagcta taaaatggtt agctactggt gctcagccaa cagaagtagt taaaaaatta
    15721 cttgtaaaaa acggagtaat agaaaaattc gaagcttcta aacaagcaaa ataatttttt
    15781 cttggcaatt agtttaagta agggctcatt gctaaggtta ctttaggaga tgaaatatat
    15841 gaaagagcta gttgtagata tagctaaggc tctagttgat aatcctgact cagttgttgt
    15901 tgaggaattt gaagataatg atggtatcgt cttaaagctt acagttgctc aagaggatat
    15961 gggtaaggtt attgggaagc agggaagaat agctaaagct ataagaactg tcgtaagatc
    16021 tgttgcaaat agagaaaata taaaagtttc tcttgagata gtataaaata ggaggattag
    16081 gtataatgcc tagtcctttt tatataaatg cgtagtttaa gattttatga tgatatgacc
    16141 acataagatt aaatttttta agaaggtgat aacatgaaag aaaaattaac tcattttaag
    16201 gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag taagagttta tccgcttact
    16261 gatgatatag agaggtttga tgaattaaaa gatttctatc tgggaaaaga tttaaataat
    16321 aaattggctg ttgaaaaagt aagatacaaa ggttctatgg ttataatgaa gataaaaggc
    16381 atagacagca tagagaaagc agaaaaacta aaaaataaat ttatgtatgt agctagagaa
    16441 gatagcagag agcttgaaga aggcgaattt tttatagcag atatgattgg aatggaagtt
    16501 ttaactgtag atggaaaata tgtagggata ctagaagatg ttcttcaata ctcagcaaat
    16561 gatgtctatg tagtaaaagg tgaggaagat aaagaattta tgataccagc tataaaaaaa
    16621 tttgtaccta caatagatat tgatgaaaga aaaatgatta tagaccctat taagggaatg
    16681 atagattagg tggtaatatg agatttcata taatgacact ttttccagaa atatttaatt
    16741 cgtacatgga tgaaagtata atgaagagag cagttgaaaa aggtataatt gaggttcata
    16801 tatataatat aagggatttt tctaataata aacataaaaa agtagatgat tatccatttg
    16861 gaggaggagc ggggatggta atgactcctc aacctatata tgacacatat aagcatataa
    16921 taacaactca taatataaat aacccaagtg taatttatct tactccaaaa ggtaaggtat
    16981 acaatcaatc tatggcaaag cagatgtctt taaaagaaga tataatacta ctatgtggtc
    17041 attatgaagg aatagatgag agaatcattg atttaatagt tactgatgaa atttcaattg
    17101 gagattatgt attaactggg ggagagctcc cagcacttat aatgatagat tctatatcga
    17161 gattaatacc aggtgtatta aaccaagaag aatcatttga agaagaatca tttaaagaca
    17221 acttattgga atatcctcat tacactagac ctagagattt tgaaggatta aaagtaccag
    17281 aagtgctttt atcagggaat cataagaaga tagatgagtg gagaagagaa gaatctataa
    17341 ggataaccaa agaaagacga tttgatttat ataaaaaatc taatgaaaaa taaaaatata
    17401 atgaaaaatt aatatatatg tatacaataa agtacctata aatggaatta aaaacataaa
    17461 agtgcttgta ataactattg aagtatagta taatataaaa tgttgaaaat acgggcgttc
    17521 ctctgtttac actggtcggg tagttaagaa cgtctatgat atctaggagg aagaaagatg
    17581 aatgaaatgc taagagcaat tgagcaagaa caattaaaaa atgaagttcc taattttgga
    17641 cctggggaca cagttaaagt acacgttaga attatagaag gaaaaagaga aagaatacaa
    17701 gtattcgaag gtgtagtatt aaagagacaa ggtggaggag caagagaaac tttcacagtt
    17761 agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc cagttcactc tccaaaaata
    17821 gaaaaaatag aagttactag aaaaggtaaa gtaagaagag ctaaactaaa ctacttaaga
    17881 ggtagagtag gtaaagctgc taagataaaa gaagctagaa ataaataatt aaaaacttta
    17941 cttataaatg gggggctgat ttatcagtcc tctttctatt ttaatgaaaa ttacagcaaa
    18001 taattatact agaaataaat agaaatattt gttgaaaata tacattattt atctatgaat
    18061 ttatagcttt taaaatgaaa aataaaattg gtaaatattg cattatattt tgtaacaata
    18121 ttaaaaataa gagaacagta ttatgtaaag tatgacgtta aaaagatgac aatattatag
    18181 tttgttaaga tagatttttt aagtggaatt aggatataat aatataatat taataaggag
    18241 tgatacatgt gtcaaatgaa tacgaagagt atcttatgaa tgacaatcta catataaact
    18301 ggtatccagg tcatatgaaa aaaactaagg agttagtaaa aaataactta aaattgattg
    18361 atgtagtaat tgaacttttg gatgctagaa tcccgtttag tagtaaaaat ccagacatag
    18421 acaggcttgt aggagataag ccaagagttg tcgtacttaa taagagtgat atggcagata
    18481 gggataaatt gaatcaatgg attgaatact ataagaaaat taatatcaaa gctataccag
    18541 tagatacaat aaaaggcgtt ggtataaata agataatcga agaatgtaaa aatgtaacta
    18601 gagaaaaaat gagttcttta aaagacaaag ggagaaaaga aagagctata cgaataatga
    18661 tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa caaattaaca ggtagaaaaa
    18721 gcactcaaac tggagataag ccaggagtaa cgaaagggaa acaatgggtt agattaaagg
    18781 gaaatcttga acttttagat acacctggaa tactttggcc taagtttgaa gaccaagaag
    18841 ttgcattaaa tttagcattt agtagagcta taaaggatga gattttagat actgaaactt
    18901 tggctttaag acttatagag aaacttatga aaatagaacc tgagaagttg aaagccagat
    18961 ataagttaga ttgtctagga gaaactccaa ttgaaactat ggatatgatt ggccataaaa
    19021 gagggtttat aacaggtaaa aaagaactag attatacacg catagccaca actgtattaa
    19081 atgaatttag agatggaaaa ataggaaata taacattgga agttccagaa aacgttaaaa
    19141 gatagatttt aaaacaaaat caaaaattta atgttaaaaa tttaaaaaag ccttttagca
    19201 aaggcttttt tatttttttg aaatatagac aaatatctcc atataaaagt ataaattagg
    19261 ttagaaaacc tttaaaaata ttaatttaag acaaatatac ctaatatttt gaaaattaaa
    19321 atgagtgtga ttatttaaaa ttttagcata ttgacttgct tgtttcgtat aatatataat
    19381 aagcacaaat aaatacaata tattaaaaat ttaatttatt taattttatt aatttaataa
    19441 taagttctta ttgtttaatt caacttactt atgcaagcgt aaacaatcga gacattttat
    19501 taatttaata aggttaaaaa gggggatttt ataatgggta agaccaagga tgttatagta
    19561 tttggatttg cgttatttgc aatgttcttt ggagcaggta acttaatatt tccaccttac
    19621 ttgggaataa ttacaggtcc agaatggtta attgccttct taggatttac gtttgctgat
    19681 gctggattgg cacttctagc agtaatggct acagctaaat tcgatggaaa cgtagttgaa
    19741 atgtttaaaa gatgtggtat gaagttagga attcttatag gttgtgcaga tattttatgt
    19801 ataggaccat tcttagctat accaagaaca ggtgcaacaa cttatgaaat gggtataatg
    19861 cctttgtttg gaacttcaat tccagtgtta ttattctgta tacttttttt tgcgatttca
    19921 tatgtactaa caataagacc ttctaaggtc gtagatatag ttggacagtt tttaactcca
    19981 gctcttctaa tagctttagc atttataatt ataaaaggta ttatatcacc tcttggagat
    20041 atagtagata aacctatgat acctaatgta tttgcagaag gtataggtca aggatatcag
    20101 acaatggatg cttttgcagc aatagcactt gcatcagtgc taatagtatc tttaaatgat
    20161 aaaggatatt ctactattag tgacaagttg aaaatgattg gtaaggcggg tgttcttgct
    20221 tgtggaggat tagctctagt ttatggagga ttatgtttcc ttggggcaac tgtatcaaca
    20281 atgtatggaa cagatgcagt acaatcacaa gtaatagtaa atataactga aggattgctt
    20341 ggaaatgttg ggaaagcgat tttagcagta gtagtatcat tagcatgttt aactacatct
    20401 ataggtctta catctgccac aggtcaatat ttctctagac ttacaaaagg taaattgagt
    20461 tatgaaaaaa tagtattagc tgtatctgta ttcagtgcag tagtagcatc ttttggtgtt
    20521 ggaactataa taaagatagc atctcctata ctaagtatag tttatccacc atcaattgtt
    20581 ttaataattt tagctttctt caatgaaaaa attaagaatg ataatgtata caaaggagca
    20641 gtatatatgt cattgtttgt aagtatacta acagttatat ctagttatgg agtagctgtt
    20701 ccagtagtaa attctttacc tcttaattca ttaggattta actgggtggt gccagttata
    20761 atagctggaa taattggtaa ttttatacca tcaaagagtc aatctaatac acttggaaca
    20821 aactaagttt tgtttaatta aaatataatc aattgtaaat aaatgagtta actaaaagta
    20881 aaaggagtta tttgtacaaa taactccttt tatttttaga ttctatattt aattatttgt
    20941 atttaatatg gaaaaagtat aagaaaatat caaaaaaata tatggtttat tttttaacaa
    21001 taatagagat taaaaaagaa ttttagagta attttataaa tttaagtata catattaaat
    21061 gatttttaca aaaatataat ataaataaaa ttgtgacaat aataaaataa taaaacaaaa
    21121 tttaagacat atgattatat aaatgtatta aataaatgag taaattgttt tcattgagag
    21181 atatattaag aatttttagt taattatgac atataagtat gtaaacgtga atactagcaa
    21241 ctgcaaagtt taagtaagtt ggaagttaga gagtaattgt tatgaagaaa aataagttga
    21301 taaatcataa taaggaattt tcagaaaaaa tggttttaag agtaaaaaac aagaggcaaa
    21361 tctacgcaag tagaataaaa attgaataaa gttaactgtc aatttagcaa aataaataca
    21421 aaatactaca agaattaacg taaataggtg ttatgataaa aggaaaacga ttttttaaga
    21481 atcataattt tgaaaattaa aaaaattaaa aatgctattg actgcttttt tttcagatta
    21541 tataataaac acaaaatatt taaaatttaa attg
//
LOCUS       DAELTA010000057        21409 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.057, whole genome shotgun sequence.
ACCESSION   DAELTA010000057 DAELTA010000000
VERSION     DAELTA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.057"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(228..488)
                     /locus_tag="KPG13_001795"
     CDS             complement(228..488)
                     /locus_tag="KPG13_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG0985528.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            705..1988
                     /gene="hflX"
                     /locus_tag="KPG13_001796"
     CDS             705..1988
                     /gene="hflX"
                     /locus_tag="KPG13_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0985529.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            1996..2673
                     /locus_tag="KPG13_001797"
     CDS             1996..2673
                     /locus_tag="KPG13_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG0985530.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2759..3202
                     /locus_tag="KPG13_001798"
     CDS             2759..3202
                     /locus_tag="KPG13_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0985531.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3507..4151
                     /locus_tag="KPG13_001799"
     CDS             3507..4151
                     /locus_tag="KPG13_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG0985532.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(4237..4614)
                     /locus_tag="KPG13_001800"
     CDS             complement(4237..4614)
                     /locus_tag="KPG13_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0985533.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4614..5000)
                     /locus_tag="KPG13_001801"
     CDS             complement(4614..5000)
                     /locus_tag="KPG13_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG0985534.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5179..5934
                     /gene="nadE"
                     /locus_tag="KPG13_001802"
     CDS             5179..5934
                     /gene="nadE"
                     /locus_tag="KPG13_001802"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG0985535.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            6017..6736
                     /locus_tag="KPG13_001803"
     CDS             6017..6736
                     /locus_tag="KPG13_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0985536.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(6903..7907)
                     /locus_tag="KPG13_001804"
     CDS             complement(6903..7907)
                     /locus_tag="KPG13_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG0985537.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8173..9069
                     /locus_tag="KPG13_001805"
     CDS             8173..9069
                     /locus_tag="KPG13_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG0985538.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9190..10452
                     /locus_tag="KPG13_001806"
     CDS             9190..10452
                     /locus_tag="KPG13_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985539.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10528..11721
                     /locus_tag="KPG13_001807"
     CDS             10528..11721
                     /locus_tag="KPG13_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0985540.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12350..13144
                     /locus_tag="KPG13_001808"
     CDS             12350..13144
                     /locus_tag="KPG13_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG0985541.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13239..14579
                     /locus_tag="KPG13_001809"
     CDS             13239..14579
                     /locus_tag="KPG13_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0985542.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14716..15948
                     /locus_tag="KPG13_001810"
     CDS             14716..15948
                     /locus_tag="KPG13_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG0985543.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            15988..17130
                     /locus_tag="KPG13_001811"
     CDS             15988..17130
                     /locus_tag="KPG13_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0985544.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17141..17935
                     /locus_tag="KPG13_001812"
     CDS             17141..17935
                     /locus_tag="KPG13_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0985545.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            17946..18968
                     /locus_tag="KPG13_001813"
     CDS             17946..18968
                     /locus_tag="KPG13_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0985546.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19067..20443
                     /locus_tag="KPG13_001814"
     CDS             19067..20443
                     /locus_tag="KPG13_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0985547.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20775..21119)
                     /locus_tag="KPG13_001815"
     CDS             complement(20775..21119)
                     /locus_tag="KPG13_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985548.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         8045 a         2527 c         4108 g         6729 t
ORIGIN      
        1 taatattaat tttgttagta agtatagtac tgtttgtaat gtataaatta tgtaaatctt
       61 tatcaataaa aatatttcaa tgttaaatag agggatattt ttatttccaa aataagataa
      121 taaaaaagga ttagattaaa agttatacaa taaaaatagc tgtgcaataa tatttagact
      181 atgaaagtag ttgaaaaata tttatgcaca gctatttata atagaattta ctttttacta
      241 gtgattttaa ctaaatcttg aaatttactc ataatgtaat tgtaattatc ttttttatga
      301 ggtaatatac aactactgca atctttgata cctttatctg tatacctaaa gtttccacca
      361 cagttttctc ctaaagcata caaaggacaa tagcaaaata gacagttaaa ctcttctggt
      421 ttattagttt tatgacaagg gaaaaattcg caatctttgt gattaaaaaa cttataattt
      481 tcgcccatat tttcctccta attaaataaa atttaaattt tttatactat ataagatttt
      541 ggtgataata taattatact aaataatttt atgaatagtc gataatatag tattattaat
      601 catagtatta atggtataat tagtcaacag aaaaataatg taaaaataat gtaagtgtat
      661 tttgtaatta gaatatgttt tataacaaaa aatgaggtga ttaagtgaaa gaaattcaag
      721 aaaaagcact tcttgtaggt ctgaatttga ctacaatagt gaaaaaaaat gatgatatag
      781 acactaatga atctatggaa gaattaaaag aactaacaaa agctgctggg gcagaagttg
      841 taggtagctt aattcaaaat aaacactctg tagatgcagc ttattatata ggaaaaggta
      901 aagtagaaga aataagagcc tatagtgact ctttagatgc tactttagta atttttaatg
      961 atgaattatc tggtgctcaa atcagaaata tagagaatgt tgttggaaga aaagtaatag
     1021 atagaactac tttaatttta gatatatttg ctcaaagggc acttagcaaa gaaggtaaat
     1081 tacaagttga attagcacaa ttaaaatata gattgccacg actttatgga atgggtggtg
     1141 agatgagtag aacaggtgca gggattggta ctagaggtcc tggagaacaa aaactagaaa
     1201 tagataagag acatatactt aataaagcag cagatataag acgtgaactt aaagaagtaa
     1261 aaaagaatag agagacacag agagttaagc gtttaaagtc aaatataccg attgtagcac
     1321 ttgttgggta tacaaatgca ggaaaatcta ccctgttgaa cgaattaata aagacacaca
     1381 aggattatga acaagaaaaa gaagtttttg taaaagatat gctttttgca actttagatg
     1441 taactttgag aaaggctctt ttgccgaata aaaaagaatt tttagtagta gatactgttg
     1501 gatttgtaag taaacttccc catgatttag tagaagcgtt taaggcaaca ttggaagaag
     1561 tacagtatgc aaatttaatc ttacatgtaa ttgatgcaac aaatactagt tatgaattgc
     1621 aaaaaagtac gactgaaggt gtattaaaag aacttggtgt aaatgataaa aaacatatac
     1681 ttgtatataa taaagtggac aagctagaat tggatatata ccctaaaagt caagaagata
     1741 ttgtgtatat ttctgcaaaa caaggcataa atatggataa gttattaaat atgatagaaa
     1801 ttgctttaat ggaaaatact tattcagtaa gtttgatgtt accttatgag agaggtgata
     1861 tatttagtag aataaaagat aaatataatg tggagaattt tgagtatggg gaaagtggta
     1921 taacgctaga tgcagattta gatgaagaag attttaatat ctatagagag tacatactag
     1981 aaaaataggt tgaatatgga tataaactgg gaaaatatag ataatcttga agattatttt
     2041 ataacctatc ttctttataa agaatctaaa acagtatctc aaattagtaa aattagaaat
     2101 atttcaagta ctgaagcaag tgagcaactt atacaagcaa agttaaaaat aaaggaaatg
     2161 caaaaagatg attttgaggc atctaaggat attttggata aatttctaga actagataaa
     2221 attaagagac ttgattttat ggatagctta gatgacgaaa aaatggtata ctttaagaga
     2281 aaggttttca aaagaatcct tgtagaaaaa aatgctgaag atttaattgt tttaatttgg
     2341 gcgacaggtg agctaaaaga tgataggttt ttgaaattgc ttcatcagct cacaaaccac
     2401 agacactcag atattagaag aataacttat tcagcaatca gaaagattga atcaccaagt
     2461 agtagagaag tactgcaaaa aggtctctac gacaaaaatg cacagactag gcagtactgt
     2521 gcaaaagctc tttcaaaatt gggagatgaa aacagtttaa agattttaca gcagttgaaa
     2581 gacaagaata agaattttga aaaagaatat gtgcttagag cttatgacga agccattaag
     2641 tcattggaaa atgcaaaaaa tttgaaaaag taatttttta ttaaagcaat ttgtttactt
     2701 aatgatgtat ttctaaagtt taacaaggta ataaataaat taatgggagg ggtttcaaat
     2761 gatagtaaga cgttgtgcag gtggagtagt gttttatgct aataaagtac ttattgtaaa
     2821 aaatgatagg ggtgaatgga cacttccaaa aggaaagata ctaggagggg gacttccata
     2881 tgagagtgct gttcaaagag ttaaagtaga aactggcata gatgctaaaa tgatagatgt
     2941 ggctggcgat actatgtatg aatttttctc aagaagtaga caacaagaag tttgtaatgc
     3001 aataatgtgg tatgttatgg aagcttgtaa cactgattgt gtacttgctc ctgaatttca
     3061 agaaggtgga ttttataaag taaaggatgc tttagaaatg ttatcacatc ataaagagca
     3121 agctttagta gaagtatctt ataaaaagtt taaggaatta aagaaattag tttcagaggg
     3181 cgatgatgtt ccagcaaatt aattgtgaat ttagaggatt ctgaggaagg gatagcccct
     3241 tcctttttta attgaagcag ttttaagcgt aaattatata gtctctttaa actatgattt
     3301 atcaatttag agatttagta gtttatattt aaaatcagta agatatattt aaaataaaaa
     3361 ttaaaaagta tacaatttta ttatataatt gtcttatgat gttgattaaa gaagtattaa
     3421 aagtccattt atttttaaat agaatgattt tataaatata tatttgatgt gtatattaga
     3481 aaatgattaa aataggagga aatacaatga gcaattacag gacattacat gaatttggaa
     3541 cggatgaaat aacgattgaa aagtctgttt ttattggata tgctaagcct ataaagagtg
     3601 aagaagaagc attagagttt ataaatgaaa taaagaaaaa gcataaagat gcaactcata
     3661 atgtgtgggc ttatactgta ggtaaaaata tgaatataca aagatatagt gatgatggag
     3721 aaccacaagg aacagctggg atacctacat tagaggttat aaaaaaagaa gatttgagag
     3781 atgtagcggt tgttgttact aggtattttg gaggaacaaa attaggtgca ggtggtctag
     3841 ttagagctta tacaaaaggt gctaaactgg gacttgaagc aggtaagata atttataagg
     3901 taatgtatca agaagttaag gttaagatag attatactca acttggaaaa gtgcaaaatg
     3961 agttaatgaa tttggggtat tttataaaag atactgtata tgaggataat gttgaaatag
     4021 tagtatattc aagattagaa gatgtagaga aattatcaga aaaaatgatt gatataacaa
     4081 gtggaacagg aaaaatagtt ttaggagagg aattttattt gtctgaacaa aatggagaaa
     4141 ttttaatata atctaaaagg ttattagaat agttaatacc tcaaatagcc aattagtatt
     4201 tttaagtgaa aagccattaa attgatttaa tctaaatcaa tttaatggct ttttatcgga
     4261 ttttgcacag aatccttttt ttacttcaaa taaaccgact aaaagtaaaa atatcccaaa
     4321 tatttttttt aatatatctg aagacagata attagctaat agtgaaccaa atacagctcc
     4381 taaaacccca aacacagcta taggtataac caatttaaaa attacatttt tattttttat
     4441 atgtatagca agtgctataa gtgtcatagg gatagatgaa agtaaattta cgctttgtgc
     4501 tattttagtg tctatgccag caagtaaaac gagtgcaggt ataaggattg ttcctccacc
     4561 cattcccatt cctccaataa ttcctgcaaa aaatccaatt agagcaaata acattaaaac
     4621 accatcctta atgctgcaat tatcataatt attccaaaag aaattctaag aaactttcca
     4681 gtaaccttat ttagtaattt agctcctata ataccaccaa gaatacttcc aactgcaact
     4741 ttaattgtta agttaacatc ataagtacct ttagatacat ataaaacaga acttgctgtc
     4801 gttaagaata ttattataga aagagcagtt gcatgagatt tatgttcttc taccttaacg
     4861 atgtcattta atattggaac taaaagagta cctcctccag aaccaaaaac tccatttata
     4921 aatcctgtaa aaactccaat tatcgtattt tttaaattaa aatcaaagta ttttttcttt
     4981 aaatcatcag tttttttcat aaaaatcacc ttctgaaata gttttaccaa aataggcaaa
     5041 ttatatttat ataaaataag attaaatata atctttcatg tttgtttaat tttgcataaa
     5101 aaaaaacata ttaaaacaac atattaatat atggtataat atattaaaac aaagtagcaa
     5161 atttgagagg agataacaat gtcaaatata aaaatacaaa tagacaaaac tgtagaatgg
     5221 cttataaata aggttaatga ggcaaatgca aaaggattaa ttgttggtgt atctggagga
     5281 attgattcgg cagtagttgc taatttaatt aaaaaggcat ttccagaaaa ctctatggga
     5341 gttataatga gcataaaaag taatcctcaa gatagagaag atgcacttaa ggttatagag
     5401 ggatgtgata tagaatacct tgatttagac cttatagaac cacaaagtgc tatattagac
     5461 atggtagtag gaaatttaaa agataaacat ttatatagag aagaatattt aaagatgaca
     5521 gatgcaaact taagagcaag agtaagaatg agtacaattt atacaattgc taacaatcta
     5581 ggatatctgg ttgttggtac agacaatgct gcagaaatac acacaggata ttttactaaa
     5641 tttggtgatg gtggagttga tattttacct attgcaaatt taactaaagg agaggtgtat
     5701 gaatgggcaa aagagcttgg tgttcatgaa gatttaataa acaaagctcc ttcagcaggt
     5761 ctttgggaag gtcaaacaga tgaagatgaa atgggaacta cttacaatat gatagatgct
     5821 gtattagaag gtagattgga tgaagttcct aaaagagacc aagagataat tgaaagactt
     5881 catagattaa gtgaacacaa gagaaaaacg cctgcacaac ctcctaagtt ctaatttact
     5941 atttataaga tttgtgataa aatttacaag tgatgaaaat tagaataatt aattgtacgt
     6001 attaaggagt ggaaatatgg gacgtatagg taatataatt aatagaaaag gtaaacaaga
     6061 tgctcaaaga gcaaagatat ttactaaaca tgctagagca atagcagttg cagctaagga
     6121 aggtggagca gacccagagt acaatgcagc gttaaaaaca gctatagaaa aagctaaggc
     6181 agataatatg ccaaatgata atatagatag agctatcgct aaaggtgctg gagcaggtgc
     6241 tggagaagac tatgaaacta tagtttatga aggatatgga cctggtggag ttgctgttat
     6301 agtagaaact ttaactgaca acaaaaacag aactgctggt aacgtaagat actattttga
     6361 caaaaacgga ggaaacttag gaactagtgg atgtgtgtct ttcatgtttg ataaaaaagg
     6421 acaaatatta gttggtttag gagatggagt ttcagaagaa gaattgatgg atgttgcatt
     6481 agaagcagga gctgaagatt tcataacaga agaagatggt tatgaaataa taactacacc
     6541 agaagatttt tctagtgttc gtgatgagtt aaaagctaaa ggatatgagt ttatatctgc
     6601 tgatgtgaag atgatacctc aaactacaac tgtattgact gaagaatctc atttaaaaat
     6661 gatgaataaa ttagttgata tgcttgaaga agatgatgat gttcaagata tatatcataa
     6721 ctgggaagta gaataaatag tataagcata ttgaaattta taagtattga atcaatttta
     6781 acatgtgata aattttatag aataagaaac actaaaatag acatacgcac ctctaaccta
     6841 tatttgcata ataatatgta agcataggta aggggtgttt ttatatttat aggttaagaa
     6901 atttaaagta aattttgagc tggactcgat tgtctataaa taattgatgg gtatataact
     6961 tgttgctgaa taccatttat acgcccagtt actgtatcaa taatcatcct aagactacgt
     7021 tctgctgtct cttccatttt taaatctata actgtcaaag gaggaatact tcttgccatt
     7081 agtcgtgaag gtccattact tgctgcaaat atttgaatat cctctccaat tacaattccc
     7141 gattcatgaa atgcattcat cataccaaga gctaccacat catgattaca aaaaattact
     7201 ttttttagtg aattttttgc aagcattttt tgagcaatat catatccact atctatttta
     7261 ttagaacaat aatatataga atctgataga tgtattccat gttctttaca tgtttttaaa
     7321 aaagatttac cccttaaagt catacatgta aaggaagttg ggttgaatac aacagcaata
     7381 tcatttttac cattttgaat agcatgtaaa gctgctatac gaccagtttc ctcattatca
     7441 atagatacac ttgaaaaacc aggtatttca cgatttacaa caactacagg aattttaggt
     7501 ttatttttta ctagatattc tatatcatca gcattgatac ctgttaaaac aattccatca
     7561 taataattat ttgcaaaaaa gtattcatca tcacatagat gaccaacttt gtaaggtcgt
     7621 atgatgatat ttatgtttaa atgttctaaa gaaattatgt ggtttattgc ttgaagtata
     7681 ttggttgatg ccaactcaaa tccctcatcc tgccaataaa aagctatttt aatattatta
     7741 tgtttaatag gagtagagcg tttatatcca agcttatcag ctgaatttaa tataagctct
     7801 tgtgtatgta gcggaatcct gcgttcattt cctttaccac ttaagacgta cgagacagtt
     7861 gctgaagaaa gaccacagtc ttttgcaata tctttgattg ttgccatgat aaaatcctcc
     7921 ttaaatagtt gcttaaattt gcttatacca ataatcttaa catatattca actaaagaac
     7981 aatattctac aaggtgcata ttttacagga ttagaataaa taaattaaaa aaaacttctt
     8041 acttatgttg acataattta gtttatagga ttataatatg cttaaataag cttatgatta
     8101 acttgaatta agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgcaaa
     8161 gggggaaaag gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa
     8221 aacctgaaag agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct
     8281 tctgggatta aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg
     8341 aaagatgcaa aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat
     8401 gcattagttc caaatttata tggtgctaaa gttgcatggg agtgtggtat aagagatata
     8461 tcctatgtta tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa
     8521 gagtctattg atgagcttaa aaaaataatg aatacttatc cagacatgaa tatatgtatt
     8581 ggaatggcta ctgtatttgg atgtcctttt gaaggtttac caaaaattga agatgccata
     8641 agatttgcta aacgcctttg ggaaaacggt atcagggaga taaatctagc agatacaata
     8701 gggattgctg accctaagca agtaagagaa actattagtg cattagttga agctttacca
     8761 gaatgcagat ggcaagtaca tattcatgat actagaaata tgggaatggt taatactctt
     8821 gtggctattg aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc
     8881 ccatttgctc ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa
     8941 cgtatggggt atgatacagg agttgatttt gttaaacttc ttgaattatc aaaatatcag
     9001 aagagttttg taaatggagt atatagtggt catcatgtaa atataaagtc tgatagttgt
     9061 aattgttagt tctattgatt aatcgattaa aaaaatatgc tgattggtaa aatagattta
     9121 ttgataaaaa atataaatta taaaatacat tatctaataa aacaaataaa aaaattgagg
     9181 gggattagta tggatattat agctttactt ttatttttag gagttattat tattgcattt
     9241 gtacgtaaga ataatgcagg tattttggct ctagctattg gtgtcatagc tgttagaatt
     9301 tttggaatgg atgataaaga actttatgga gctataagca actcaatgtt tactacactt
     9361 gttggtataa cactactctt tgcaatagtg aactcaacag gagcattaaa gcttgttgca
     9421 gaaaaaattg tagctgcttc tggtaaacgt gtttggctta tacctatagc agtttttata
     9481 gcaggatttg ttgttgctgg tgttggacct ggggcaattc cagcacttgc aattatccca
     9541 gcaatggctg ttcctgtagc cataacagtt ggttataatc ctgtaatgct tgcacttata
     9601 ggtgaatgtg gattaatggc aggtcgtatg actcctatta ctccagaagg tcaaattata
     9661 acaaccgttg cagaaggggt tggaattgaa aatgttatgc ctactatctt agcctgtcaa
     9721 actctttcta caattgtatt ttcaatagta ttgtttataa ttttcaaagg atataaactt
     9781 aaaaaaccta taaatgttat agatagtaag caaatagaaa aattcaatat aaagcagata
     9841 atttctctaa taagcatagt agttatgatt gcttttatca taatttttga tatgaatgtt
     9901 gggcttgcag cttttgcagt ttcagctgta cttatttttt tcaatattgc tgatgaagga
     9961 gaatgtatca aatcaattcc atggagcacg atagtaatgg ttcttggagt aggtgcaatg
    10021 atgacaatcg tagatgcagc aggaggtatt gacttgatga gtaatgcatt atcatcactg
    10081 atgaatagta aaacagctac tccaattatg agtatttcag caggacttat gtctatggtt
    10141 agttctgctt tagcagtagt ttatccaact atgatgccta tgtgtgttga catagctaag
    10201 gcagttggag gagtagaccc actagcactt attgctgcag ttggtgttgg aggttctctt
    10261 gctggtgtaa gcccactttc tactggtggg gcattgattt tagcagctat gggttcaagt
    10321 aagaaagact ttaataagga agaacaaaat aaggtttttg tacagctttt cattatgagt
    10381 gcagttggct tattggttat tgcagtagta tcaatattat catttaatgc aataacacat
    10441 ttaatgaact aattagaata actgaaaata aatcattaaa tttaactttt aatattaatc
    10501 aatataatat aatttggagg ggaagttatg aaaaaaggag cattaagcaa tataaaggta
    10561 cttgacctta caagagttct tgcaggtcca tattgtacaa tgatgttggc ggattttgga
    10621 gctgatgtta taaaaataga gatgccaaga aaaggtgatg atacaagagg ttttggacct
    10681 tttgtaaatg gagagagttt atactatgca aatataaata gaaataaaaa aggtatcact
    10741 ctaaacctta aatcagaaga aggaaaaaat atatttttag aaatggtaaa acatgctgat
    10801 gttgtagtag aaaattacag accaggtgta atggaaaagt taggtctggg ttatgatgag
    10861 ttggctaaag ttaacaatca aataatttat ggagctgtat caggatttgg atgttatgga
    10921 ccatattcag aacgtccagg ttatgatatt attgcacagg caatgggtgg tcttatgagt
    10981 ataacagggc aagaaggagg agaaccaacg agagttggta atgctatggg tgatgtacta
    11041 ggtggaatga atttaactat tggtatactc acagcactca acgctagaaa aataatagga
    11101 aaaggacaaa gagtagatgt ttccctcgtt gattctgttg tatcgtctct cgaaactggt
    11161 atacagagat actttgattc aggacaacct gaacttatgg gtaatagata tgcttcagcc
    11221 tatccttatg actcttttaa ggcaagtgat ggaagttttg taataggctg tggtaatgac
    11281 aaactttttg aaaagttatg taaaaaagtt ctttttagag aagacctttt aatagatgag
    11341 agatttaaaa caaatcttaa tcgttgtgaa cattacaagg ctcttaaacc agaaatagaa
    11401 aaatggtctg ttaagcatac aataaaagaa gcagttgact taatatcaga ggctggagtt
    11461 cctgctgctc ctatctataa tcttgattgt gttacaacag atgaacatat agcaaaagca
    11521 agagaaatga ttgtagatat tagtcatcct attataggaa atatgaaagt caatggaaat
    11581 ccagttaagt tgatgacaac aaaagctaaa attgatatgc cagcaccatc tctaggggaa
    11641 agtaacgagg agatttataa tggttggctt ggttttgata gtgataaaat aaaagattta
    11701 aaagaaaatg gagttatttg attaaaaaat agctaaacac acttgtgact ttagtcatgg
    11761 gaggttcagt tagaataata tcaaaaaata ttaaatcaat actgtaaata aaatatagaa
    11821 ttgtcagaag catttaagat gggaaaagct acttagacat tgagtctttg tggcttttcc
    11881 tattttcatt aagaatttta tattatcaga atagatatgt actaaactaa agtattaaca
    11941 atttaaatta atagaaaata aactacctaa ttatgttgaa taaagactac tttagtgaaa
    12001 ataaacttat agagatgcta tttaaattat tttaatggaa atatcaaaaa tgagactgca
    12061 ttctcaaaaa tgagactgcg ttttttaagt attttctcga aaatgagaca aaaatattgt
    12121 tttatgggtt ttattgaaga aaaattcttc tgtgaattac aatttaatca attttaaagt
    12181 tgaaaattat taaaaattta gtgaaattta tgtatatttc gtttatttta ttaaaaataa
    12241 caatttttta ctaaaaataa agaacttcaa tggtgattgg catagtactt gctattttat
    12301 attaacaaga aatgtagaaa aaataacaat gtataatggg gggaatatga tggaatctgt
    12361 aatggaaaat ttgaacaatc ttaaggtgga ggttaaggac aaagtctgtg ttattactat
    12421 aaatagacct aaagctttaa atgctcttaa tagtgacact cttagggaac tatcacaagt
    12481 tattgatgta gtatcagaaa atgaggcaat attaggagtt attattactg gagaaggtaa
    12541 agtttttgta gccggtgctg atatacgcca gatgcaaaac tataaaagtg aggaaggcag
    12601 aaaatatgca ggttatgctc agggaatttt tgataaaatt gaagctcttg agaaaacagt
    12661 aatagcagca gttaatggat atgcattagg tggtggatgt gaacttgcta tgagttgtga
    12721 tattagaatt gcatcagaaa aggctatatt tggacaacct gaagttaatt taggagttat
    12781 tccatgtttt ggtggaactc aaagattatc aagacttgtt ggaactggaa tagctaaaga
    12841 gttaattttt acggggagac aagtaaatgc agaagaagct aaatctattg gacttattaa
    12901 taaagtggtt ccaagtgatt tacttttaga agagtctatg aagatgatga accaaatagt
    12961 tgaaaaagcg ccaatagcaa taagatatgc aaaagttgtt attaacaaag ggatagatat
    13021 ggatttaaag aatgctcttg aactagagaa agatgttgca ggtttaactt ttgctacaag
    13081 agataagcaa gaaggaatga atgcatttat agaaaagaga aagccagttt ttgaaaataa
    13141 atagctatta taactaaaat tggaacaatg tagaatatat tcaaaatcta aatttcgtga
    13201 aaaaatacat atagtttaat tttaaaagga gttaatttat gaatacggga gtaattataa
    13261 gtttagtagg gattatcata gctctatctc ttctagttgt aatggttatg aagggaataa
    13321 atatttttat tatagcaatt gtatgttcat cattagttgc tataacagga agcctaaatg
    13381 tgtatgatgc acttaaagtg gattatatga cagggtttgt tggatttctt caggcaaatt
    13441 tctttatttt cctaacaggg acattaatgg gaaaaataat ggagattact ggtggagcaa
    13501 aatctattgc taagatgatt gtaagatgga ttgggaagga taaagcacta ctttctattc
    13561 caatagcttg tggaatatta gcctatggtg gagtaagtgt tttcgttgta agttttgcag
    13621 tgtttccgat agctttagaa atttttaaag aagctgattt gccacgaaga tttataccag
    13681 cagctttgac ttttggatgc tcaacttttg ctatggttgc accaggggca ccacaaattc
    13741 agaatattgt tccagcatca acattaggga cagatattat ggctggagca gtaaatggtt
    13801 ttatatcatg tgcagtaatg tttatagttg gaagtacaat tttgtacaaa atggtttcaa
    13861 aagaaaaagc caatggtggt cattttatag cacatgaatc agatatattt gatgaagcag
    13921 aagcaactaa ttctaagaga gaaaatggtc ccaatggtgt aattgcctta attccattga
    13981 tagtgtcgat tttaataatt aatgtaaaag ttaatggaaa agcgatagta ccaattgagg
    14041 tgggagtatt tattggagct ttattagtat atttattgtt aaataaatat caagataata
    14101 ctaaaatagt tggacatgtt ggtgatgcat gtaaaactac agtagtagca atatgtaata
    14161 cttgtgctgt agttgcattt ggttcagtag taaaatcagc agttggtttt gacttcatag
    14221 ttaatgctat gactaatatt cctggtcctc ctattgcagg ggctgcagtt ggaactacag
    14281 ttattgcagg tatttgtggg tcagcttcag gtggactagg aattgcagct ccacttcttg
    14341 gaccagtatt tttagccaag ggtgtttccg ctgcagcatt aactaggact atggcaattt
    14401 catcagcagc attagattca cttccacaca atggatatat tgttactgta acaaatggtc
    14461 tttgtaaaga aacacataag gatgcctata tgccaatatt ctgggtaact gttttaaccc
    14521 catttattgg aaccatagta gcagtcctac tgtttacaat gttcccggga ttgccatagg
    14581 agaaaagata aaaggtataa attatatttt gatattataa aatcaatttt caaaatatat
    14641 acaataaatt tcatgtttta atactgtaaa tatgtattta aaataacaaa ttgaccatat
    14701 aaggaggaat tgaaagtgtt aaataaacca ttagagggag ttaaggttat tgacctaacg
    14761 tattttgtag caggtccagg gacttctaaa atattagctg actggggtgc tgacgttatt
    14821 aaggtagagc caagctttgg agacccagga agaaagacag gggcaacaat gacaatgcca
    14881 atagatgatt ataataatcc tttttacagc acatataatt caaataaaag aggtttgtct
    14941 ataaatctta agagtgaaac tggaattgaa attatggata agcttttaag cgaagcaaac
    15001 gtatttgttt caagttatag aacaggagca ttaaaaagac taggtcttga ttatgaatca
    15061 ttaagtaaaa agcatccaca ccttatctgg ggtcagataa atggttttgg cgattttgga
    15121 cctgctaagg ataatgcagg atttgataca gttgcctttt gggcaagaag tggggctatg
    15181 cttgatatag cagaaaaaga tacaagtcca ataaatccac ctatagcctt tggagatgca
    15241 acaacttctt gtagtttatc tggaggaata tgtgcagctt tgtaccagca agctaagact
    15301 ggaaaaggac aaaaagtaat ggcatcttta tttgggcaag caatatggaa tgcatcatct
    15361 ttattagcat ctacacagtt tagtgatgag tatccaaaaa ctagaaaaaa tgctatttca
    15421 ccagtaataa attcttatcg ttgtaaggat ggaaagtgga tattcttaag tattttagag
    15481 catgaaagat attttgaggc actttgtaga ttatttggaa gagaagactt aataaaagaa
    15541 gagaaatttg catcttcagt agaaagtaag aaaaatgcta cagaacttat taatatttta
    15601 gatggtgaat ttgcaaagtt tactcaaaat gagatggttg aaaaacttac tgctgctgat
    15661 atagctcatg aaaaaattca acatgttaat gatgtagtga ctgatgaaca ggctattgct
    15721 aataactata tatatgaaca tacaagtaaa gatggaaata agacaatgct tgcatctact
    15781 ccagttaaat ttggaaatat tgaagttaac atgacttgtg atgctccact tataggagaa
    15841 cattcagatg agatattgag agaacttgga tacagtgatt ctgatataga gactctaatt
    15901 gaatctggaa ttgtaacaat gaataaagaa gcatgtgaaa tatgttaata aacatgtgaa
    15961 gtatgttaat aaataggagg gaattttatg gattttagat taacagaagc acaattaatg
    16021 cttcaaagag tagcaaaaga atttgcagag aatgaaatag caccaatagc tgctgaaaca
    16081 gataaaacag gtatttttcc aagggagctt tttagcaaaa tggctaaaat aggatttaat
    16141 ggaattggaa cacctgtaga gtatagtggt tctggtgggg cagatataga aaaggtaatt
    16201 gttgttactg agattgccaa aaaatgtgca gcatctgcag caatattatc aatacacaca
    16261 atatatgctc aagctatttt gaaatttggt actgaggaac aaaagaaaaa ataccttcca
    16321 atgatggctg aaggtgggtg tgtaggtgct tttgccttaa ccgaacctaa tgctggttca
    16381 gatgctgcga gagctgcaac aactgcaatt attgatgaag aaactgatga atatgtttta
    16441 aatggaacta agtgttttat ttctggtgga ggtcaagcag aatcattaat aatatttgca
    16501 ttgacagacc catcaaaggg aataaaggga atgtctgcta tcattgtcga caaaggtact
    16561 ccaggttttt caataggaaa aattgaagaa aaaatgggta ttcatggttc agaaactgca
    16621 gagttaatat ttgataactg tagagttcca aagtctaact tattaggaaa agaaggtaaa
    16681 ggatttaata tagctatgac ttgtttagat ggagcaagaa ttggagttgg agctcaagct
    16741 gtaggaattg ctgaaggggc tttagaagaa agtatcaaat attctaaaga aagagtccag
    16801 tttggcaagc caatttcagc tttgcaaggt attcaatggt atatagcaga tatggcgaca
    16861 atggtggaat cagcaaaatt attagtatat tatgctgcag atttgaaagc tagaggagaa
    16921 aaacacacta aagaagctgc tatggcaaaa tacaatgcgt cacgtactgc aagagaggtt
    16981 acaaatttag cccttcaaat ccatggtgga tatggatata tgaaggatta tccattagag
    17041 agaatgtaca gagatgccaa aataacagaa atttacgaag gtacttcaga aatacacaaa
    17101 gttgttattt caagagcagt actaggatag gaggaaaaga atgaatatag ttgtttgctt
    17161 aaagcaagtt ccagatacta atgaagttaa aataaataaa gagacaggta ctctaataag
    17221 agatggtgta ccaagtataa tcaatccaga tgatagaaat gctttagaag aagctctaaa
    17281 aatgaaagat gaactaggag cagtgattaa ggttataagc atgggacctc ctcaagctaa
    17341 aagtgcttta aaagaagctt tggcaatggg agctgatgaa gcttatttaa tctcagatag
    17401 agcttttggt ggttcagata cttgggctac atctacaatt attgctgctg ctatagagaa
    17461 ggttggaaag tatgatgtaa tattctgcgg aagacaagct atagatggag atacagctca
    17521 ggttggacca gaggttgcag aatttttagg gataccacaa gttacttatg cgaaagaagt
    17581 aaaggttcaa gatgacaaat tactagtaac tcgttataca gagacagggg attatttaat
    17641 agaagcaaag atgccagttt tattaactgc tataaaagag ttaaacaatc caagatatcc
    17701 aagtgtcaag ggaatcttag aagcttataa taatggagat gcaaagatta ctgtattaac
    17761 acttgcagat ttagatgttg atacaactca aattggatta aagggttcac ctacaaacgt
    17821 ttataagtct tttgtaccag ttaaagacaa gcataatgaa ataatagaag gtataaataa
    17881 aaaagaaaaa gctgagaaac taatagaaat actattcgat ttgaaactag tataggagtg
    17941 ttaagatgat gagagcaaaa gttaatcaag gtataaattt aaatgattat aatggtgttt
    18001 gggtaatagg agagcaaagg gaaggtaaaa ttaatcctgt tacgatagaa cttattggtg
    18061 aaggaagaaa gcttgctgac caattaggaa aagagcttgc agttgtgata gctggatatg
    18121 aagtagagaa agaagtgaag gaattacttc actatagtgt tgataaagta tattacatca
    18181 atgaccctct tcttaaagat ttcactactg atggatatgc aatttcaatt gcaaatttaa
    18241 tcgaaagaaa aaagccagag gttgtattgg ttggtgctac ttctatagga agagatatag
    18301 cgcctagaat tgcaggtaag gttggaacag gtctaactgc tgactgtaca aaacttgaaa
    18361 tagattcaac agataataag ttattacaaa caagaccagc atttggtgga aatttaatgg
    18421 ccactattgt ttgtccaaaa aatagaccac agatgtcaac agttcgtcca ggagttatgg
    18481 caaaagctgt aagaaacgaa tcagagactg gaattttaga agttgttact ccagaactta
    18541 ctgaaaaaat gattagaact agattagtag agatacttcc acaagaaaag aaatctgtaa
    18601 atctaactga tgctagaata atagtatcag ggggaagagg attaaaaaga gcagaaggat
    18661 ttgagcttat aaaagaattg gctgataaat tgggggcaga gattggtgct tcaagagcag
    18721 cagtagattc agggtggata gaacattctc atcaagtggg acaaacagga actactgtta
    18781 gaccagaact atacattgca tgtggaatat ctggagcaat acaacattta gcaggtatga
    18841 gtgattctaa atatatagtt gctataaata aagatgcaaa agcccctatt tttagtatat
    18901 gtgactatgg aatagtagga gatttatatg aaattatacc tgaaatgata gaatcattaa
    18961 ataggtaact tttagaaaac attgaatatg tagaatgata agtcttataa tattgtattg
    19021 taagacttat tatttttgct gaaggttata gtggggagat gaaacaatga aagagcagtg
    19081 gtataaggat atatttgcaa gagtattatc tatgactgat gatggtttta ttgttgtaaa
    19141 tacaagtgga gtaataatag atattaatga taaatattgt gactttttag gaaaagaaag
    19201 aaaagatata ataggacaaa atatacaaag tattatacca aatacaaaaa tgctagatgt
    19261 tatgaaaaat aagtattgtg aagaaggagc tatacatcat tattcaggtg gaaatacaaa
    19321 agaaaaaagt gtaatagtaa gtaggtctta tgtggaaaat gacaatggag aagtagttgc
    19381 aggagtagct caagttaaat ttagattgca atcatttgat gtagctaaaa agttaatgtc
    19441 agaatatatg gagctccaat attataaaga gcagtttaag gataactgtg gttttgacaa
    19501 gctcataggt gaaaatagag acttcataga acttaagaaa acaggtgtga aagcttctaa
    19561 aacaaacttt ccagtacttt taacgggtga aacaggcaca ggtaaagaag tttttgctag
    19621 agcaattcac aataatagca gtaggtcaga taaacctatg gttagtataa attgcgctgc
    19681 aataccagaa gaattattag agtcagagct atttggctat gacgaaggag cttttacagg
    19741 agcaaaaaag ggtggtaaaa aaggtaaatt tttagttgcg aataatggta caatcttttt
    19801 agacgagata ggtgacatgc ctttgaccat gcaagcaaaa cttttaagag ttcttcagga
    19861 aagtgagata gaaccagtgg gaggtcttaa aactataaag atagatgtaa gagtaatctc
    19921 tgccacaaga aagaatttaa gtaaaatggt tgaagaggga ttatttagag aagacctata
    19981 ctatagatta aatgtaataa acattcatat gatggaattg aaggacaggc aagatgatat
    20041 tttattgcta gcaaattata tattaaacaa attaaatgtt gaatacaagg aattgaaggt
    20101 tttaagtgat aaggttaaaa attgttttat taactatact tggccgggaa atataagaga
    20161 actacaaaat gtaataaaaa gtgcctatgc tgtaagtgat gatatggtca ttatgatgtg
    20221 tgaccttcca tctaagatgg ataatatcag cagagtagct caatgtaatg tggatagcaa
    20281 ttgttcaatt catgaaatgg ttgagaatta tgaaaagagc ttaattattg atgtgttaag
    20341 gaagtataat tggaaatgtt ctaaggcagc agaggttatg ggaatacaca agagtctttt
    20401 atataagaaa ataaaaaagt atgaaattga attaaataat taagataact tgaattaagg
    20461 caatgctaaa aaagcgccct tattcaaagc gaactgtata aactgtttta tgcagttaaa
    20521 acgtgtaaaa gtagatgtga agtactaatg tcaatcatta gtactttttt attatattta
    20581 taattactat ctatatttca gtatcttttg atatctaaga ttattatgtt taatttagta
    20641 tataacaaaa ccctcttagt tatggacagt tttttatatt ttaactgtct actaagaggg
    20701 gatagtttag tttaaattta gagtaaattt tatctagcat ttataaaaaa tattttgaat
    20761 agcttttttg ttatttagtc ataattttct acttctttat caagttcttt gccacaagaa
    20821 tcacacttat atatagcatt gtatccgtaa tcatattcat caactatttt cttttgtcta
    20881 aatacaccac cacactcaca taggaaacca atttttataa gtgtaacgat aaattgtgat
    20941 tttataaatt ttatatcatt gcatttatcg ttagtgtaaa atgtataaat ataagagtta
    21001 tcgcactcat ctcttaattc aaatctttca atatatgtgt tattttttaa ttcctcaaat
    21061 tcttctttag ttaaatatga tttttgttgc aattcatgta aacgcttaat ttggtccatt
    21121 atttatatcc tcccaataat tttagtattt taagcttaga taaggaatct aagttttatt
    21181 aaatttatcc caaggaatta tttaaatcat atttagtttt attaattaca acatatataa
    21241 attacacgaa aataaaacta attgtaaaaa taatatcctg tgaaaaccta aactactaac
    21301 cccttactaa cccgaaaact aaaaaaagaa aatatatcca aatataagtg atatataaat
    21361 aaagatgttt catataaatt taactttaaa tattctgaca ataatatat
//
LOCUS       DAELTA010000058        21299 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.058, whole genome shotgun sequence.
ACCESSION   DAELTA010000058 DAELTA010000000
VERSION     DAELTA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.058"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(165..845)
                     /locus_tag="KPG13_001816"
     CDS             complement(165..845)
                     /locus_tag="KPG13_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985549.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(997..2904)
                     /locus_tag="KPG13_001817"
     CDS             complement(997..2904)
                     /locus_tag="KPG13_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0985550.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2905..3657)
                     /locus_tag="KPG13_001818"
     CDS             complement(2905..3657)
                     /locus_tag="KPG13_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985551.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3973..5127)
                     /locus_tag="KPG13_001819"
     CDS             complement(3973..5127)
                     /locus_tag="KPG13_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0985552.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(5130..5597)
                     /locus_tag="KPG13_001820"
     CDS             complement(5130..5597)
                     /locus_tag="KPG13_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0985553.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5611..6099)
                     /locus_tag="KPG13_001821"
     CDS             complement(5611..6099)
                     /locus_tag="KPG13_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985554.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6113..7444)
                     /locus_tag="KPG13_001822"
     CDS             complement(6113..7444)
                     /locus_tag="KPG13_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG0985555.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7458..9023)
                     /locus_tag="KPG13_001823"
     CDS             complement(7458..9023)
                     /locus_tag="KPG13_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0985556.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9193..9978)
                     /locus_tag="KPG13_001824"
     CDS             complement(9193..9978)
                     /locus_tag="KPG13_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0985557.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10145..11344)
                     /locus_tag="KPG13_001825"
     CDS             complement(10145..11344)
                     /locus_tag="KPG13_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG0985558.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(11866..13203)
                     /gene="xylA"
                     /locus_tag="KPG13_001826"
     CDS             complement(11866..13203)
                     /gene="xylA"
                     /locus_tag="KPG13_001826"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG0985559.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13299..14849)
                     /gene="xylB"
                     /locus_tag="KPG13_001827"
     CDS             complement(13299..14849)
                     /gene="xylB"
                     /locus_tag="KPG13_001827"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG0985560.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            15057..16217
                     /locus_tag="KPG13_001828"
     CDS             15057..16217
                     /locus_tag="KPG13_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0985561.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16373..16777)
                     /locus_tag="KPG13_001829"
     CDS             complement(16373..16777)
                     /locus_tag="KPG13_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0985562.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(16798..17280)
                     /locus_tag="KPG13_001830"
     CDS             complement(16798..17280)
                     /locus_tag="KPG13_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985563.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17300..18109)
                     /locus_tag="KPG13_001831"
     CDS             complement(17300..18109)
                     /locus_tag="KPG13_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0985564.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18102..18881)
                     /locus_tag="KPG13_001832"
     CDS             complement(18102..18881)
                     /locus_tag="KPG13_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0985565.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18881..20959)
                     /locus_tag="KPG13_001833"
     CDS             complement(18881..20959)
                     /locus_tag="KPG13_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG0985566.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT         7115 a         3763 c         2244 g         8177 t
ORIGIN      
        1 tataacaaaa aataccatta ttataaacag tactaaaaaa tctaaatata ttaactctga
       61 atatttattt ttaaaaatat ctatagcaaa taagtagata tttattatac taactacaac
      121 taaaggtaga gcaatatact tagacctacc tttcaaatat tttactagca ttgtattata
      181 tatcctaccc ccttcttagt ttttataaaa tcatcaaaac ctagttcttt aactttactt
      241 ctcaatctat tcatatttac agtaagtgca ttgtctgtca caaactcatc attatcccat
      301 agagtcatca taagactttc ccttgataca accctattct tattttttaa taataatgtt
      361 aaaattttta tttcgttttt agtcaactct atacttttat ccttatactt atgttttata
      421 atccctttcc ccacatcaaa catcaaatct tcacaataca aaatactatt agaatcacta
      481 taattataga ctcttcttat tataccattt attttggtaa ccaatatttc tatagaaaat
      541 ggtttggtaa tgtaatcatc tgctccatta tttataccca ttattaaatc catatcagaa
      601 tctcttgatg ataaaaatat taatggtaca tttgaaatag ctcttatttt ttcgcaccag
      661 taaaatccat tatataatgg taaatttaca tctaaaagta ctacatctgg ttttttatct
      721 ataaattcat ctaatactac ctcaaaatca tcaattaatt ctactttaaa tccccatttt
      781 tcaagtgcta atgcaatctc ttttgataaa tcaatatcgt cctctactaa taaaattcta
      841 tacatttgta tattctccat tatcctaata tttcactatg ctatgaatta ttaatatcat
      901 aacatatttt atatgaattt attacccaaa ttatttagtt aatcaacaag gagactctta
      961 ttacaagaat ctcctttctc tttattaata tatcaactaa tttttataca ctacactctt
     1021 aaacttatta acagatactg ctccatatat tatgaatatt atcaaacata tcactaaact
     1081 tagtatatat ataccaaata aatcggtcat caataaatct cctgctaatc tcaatgcaaa
     1141 ataactagat atagttgcac ttaacattgg caaagtataa aacatagcta cttctttaag
     1201 tatagctcta tttatatctt tttctgtagt acctattttt aacaaaatct tatatttctc
     1261 tttatcttca tatgcatcac tatatatttt catatatatt atacttccag ctacaattgc
     1321 aaagactaag aataaaaaca caagcactac atatgcaaac ttcatccatt gactctcacc
     1381 ttgagcttta tatccatttt gaatataccc attttttaaa cttaaattct tttcaatatt
     1441 gttgaacatt acttgactat tttcttcttg aaataccttt gcaccataaa aatacacttg
     1501 ttgaccctta tctcttagtt taaaatatgt atcatcattt accactataa tactttgatt
     1561 aagtcctgtc cctagtactg gaacttttac ttctcccctt gaaatattat ataactgacc
     1621 atcaagtttt atcttggctt cttttgtagc tattgatgct atcgtttgag ggctttttaa
     1681 ctctattaca ttatttccac taactaatct ttcaccatac tcttttaatt ctttatcatt
     1741 gccattcact ttaagagtat tacaaaaatc actatatttc attatagtat ctgttgtatc
     1801 ataagcttct tttatatctg ttttctttaa aattgttagt tctggattta ctgagtactt
     1861 tatcttatta gtatctttta ttatattctc tatttgtttt ttattcactt catctttatc
     1921 cattgaaact atgctaatag tgtacacatt tctttgttct tgggcattct tatgtatacg
     1981 cttcatcgca actgaagcac caagagttga tattgttgta gttataatta tagctattat
     2041 agcataagtt ttatagtttt tatttaatcg gtagtttata ttgtttattg caattatatt
     2101 atttccatta aataatattt ttttattttt gattataata tcaaatataa caggcataac
     2161 tgatttgaat aatccataag tacctactat tattatacct ataacaggta gtacactagt
     2221 tatatctcca gttcttatat tggtagaaag atagtagcca tatcctatta ctataattga
     2281 tactatagct aatatatata tacctattcc tacctttggt attttttctt gttttttaga
     2341 tatattgatt agatttatta cactacttct acatattgtt ataaaacctt ttacagccat
     2401 tattataaat atagaaataa agattaaagt agcatatagt atagcttcta tactaagtcc
     2461 attagatacc tttatattaa atccagataa ctttattatc actacttgaa aaaatctaga
     2521 tgttattatc cctataatta ttcctgttat acaagaactt attcctataa gcatattttc
     2581 tacaaaatat atttttccta tagtatataa atctaatccc ataaaagaca atattccaat
     2641 ttctttagac ctattcttaa aaaatatatt tgttgcatac catataaaga aaaataaaaa
     2701 tactgatagt atcattatta cagctactaa cactgatagt gtaaattttt tattttcatc
     2761 ttgtaacaca tttatagcat caccatatct taataattca aaattagtta gcacaactac
     2821 tgaaaaaacc atagatataa aaaatgctag ataagttctt atgttactct taaaattatt
     2881 ataagcaatt ccaagtatac tcacttacat ctcacctccc atagaggcta gcatatccat
     2941 aatttttcca taaaattctt ttctactatt tcctttgttt aatttacact ttatatttcc
     3001 atcatttatc atatatactt catttgaata acttgctgtt agagggtcat gtgtaatcat
     3061 tataatagta gcgttatttt ctctgtttat tttttctaat gattctaaaa gttcatatgc
     3121 tgatttagag tctaaagcac cagttggctc atcagcaaat atgactgttg gatttgtaat
     3181 taatgaccta gctgctgcgc ctctttgttt ttgtcctcct gataactgat atggatattt
     3241 atctaaatga ttttctaatc caaatttttt agctatagaa aataccttag cttctatctc
     3301 cttacttttc ttttttccta atgctagagg aagtgctata ttgtccatta gtttcatatt
     3361 gtctaataga ttatagtctt gaaatataaa ccctattttt tctcttctta attttgatag
     3421 ttcattattt tttacttttg atatattaat tccatctagt attatttctc ctgaagtttg
     3481 tttgtcaagt gtagacatta aatttagcaa tgttgtcttt cctgcaccag atggacccat
     3541 tattgatata aaatcacctt cattgactgt aagagatata ttttttaaca catttgtttt
     3601 aaatcctttt attccataat cttttgatat attttttatt tttaatattt ctttcataat
     3661 aatagcctcc agttgctaat tttttaatat atatttattt taaaggtttt atataaataa
     3721 atccttaagt aaatattaca ttttaactgt tagcattaca cttttgtaac atatggaaga
     3781 ctattataat tatagtagaa atatcttaaa ataaaagcat gatttacttc tttgtataaa
     3841 caaagaaaag aagctcctaa gaagcctcta atctatacaa ttattcttct ctataaaaaa
     3901 ccaaaataca cattgattgt ggcaaataca gatactataa acaagtgata ttactactat
     3961 atattaaaac aattattcct gaagataatt caataagact atcaagcaat aagctaagca
     4021 tccaaaaaga cttaatacca ttaatgtata attataccca atattaatgc tcaaaaaacc
     4081 agcaacagtt ggaataatca gagatgaacc tcccataaat atacttaaag ctattaattg
     4141 gctactactt ctaagttttt taggagaaac ttcaaatatt aatctttttg aagtaattaa
     4201 aattaaagga gtagaaaaca cttgaaaaac acacagtata ataatgatat ttgaatcatt
     4261 tgttaaagca aataatagaa actgaactgt tatcataata gcacttaatt ttaataagtt
     4321 taaactgcta tattttttta ataaataaga accagccaga tatgttggta tctctaacaa
     4381 tgactgtaaa gaccacttta aagatatctg agaatttgta gcatttaaag ctatcatttt
     4441 atctattaca gtacagttat ttgctaccac cattgagtat aataagaata aaatacatac
     4501 tagtaagata tattttttat cttttaataa ttcaataata tctttattag ttacatttat
     4561 tttcttatta atcttttcaa tatcaggtaa taatagtata tttataataa gtaatactac
     4621 aattaaacca atcgctacag ataatccaaa atatgagaat ctaagaatca agtaagatgc
     4681 taccatactt ccaatagccc atccaatcga cccaaaagtc ttaataaatg acaaataatt
     4741 attaatatta tctccacaac ctattatcca tgtatcatat aaactacagc aagtatttaa
     4801 taatcctcct gataaagcaa caagtaataa gtatattata aaagttgcag attcagtaga
     4861 aaacataata actgatgcaa ttccataaac tgataaacta attaaaacaa tttttttcat
     4921 tgtttgatac ttatcagcta atactccaaa taataattga aacaatatat tggttattgc
     4981 atagctcgat aatataattc ctctttcaaa cgctgagtaa cctaattttg ataagaaagg
     5041 tgtgaatttt gttgaagcaa aggcaaaaat aatatatgag aaaaaataga atatgataaa
     5101 tttaaattta atcgctttat taatcataat caatcaaaat tgataacttt aataccacta
     5161 tttccttcct gatttaagtt tgaaagatga tattttaccg tattaggatt ctctacaata
     5221 actaaaggaa tatataaagt attttcaagt aacttattat caatttctac taacttttcc
     5281 cccatattaa cttcttgacc ttgtttaact tctaatttaa agttttcttt ttttgttgtt
     5341 gcactatcca atccaatatg aataagaatt tgaacatcat tttgcaattt aattcctata
     5401 gcatggttag tttgagcaat aaaacttatt actcctttag tcggtgattt aagaatattg
     5461 tcctttgcta tcatagcaaa tccatctccc attaccttag ttgaaaatac tttatcagga
     5521 atttctccaa gattaattat ttttccatca atgtatgaat taattgtaat tgcttctttt
     5581 tttcttttaa agaacattat agacactcct ctacataatt aactttattc taattatcat
     5641 tacaaataac gatattaagc atactgcaat tgaggaaaaa agagccttat agctctttcc
     5701 ccattgtagt tctttaatac tgcttatgat ttgtttaatt ccaacaaatt tataaatata
     5761 ggtaaacatg ataacaatag ttattgtgta aatttggaat atctgcccta ataagactaa
     5821 atagaaagca atcatgtttt cttttatctc tttattcttt tttatattgt aaataacata
     5881 tgcactgtat actgcactaa agttagagat taaatttaaa cttgcattaa catcattgtt
     5941 taaaagttgt gaaaatgaaa tatttgtaaa taatgatgtc aattctaaat acactatata
     6001 taaaacaggt aatattagaa aacaaaccat aaccactgaa agaaatatat tttcatcttt
     6061 taatactgct gtgatatttt ttattttatt tgttatcatg tttttgttct ccttatttaa
     6121 aagttggcca gaaatcctta ttagccacaa tcatttcatc taagatttct tttgctactt
     6181 ttgcacttgg aacagtttta ctcatagtaa acgactgcca taatttttga taactttttt
     6241 ctatccatgc ttctacagct aatttttcag aagcaacttg ttgttccatt aaaccttttt
     6301 ggaaagttgg aattttacct atagacatag gttcaaccat atcttttcca acaaggcaag
     6361 gaatttcaac cattgcatca tctgcaaaat tttcaatagc tccattatta ggaacaatca
     6421 gtaaaaatct ttcctgagta ttaaatgcta atgcacaagc taattcaaca ataaattgag
     6481 aatgttcact tgcatcaaac catgtatctc ttgctgtgcc attttttata attctttcac
     6541 attcttcgaa tgtatccttt tctcttctat ccataatttc atttgctctt gtataatttg
     6601 gattagcatg ttcaacagtt tcatcagcca ttaaataata ttttaaataa gtattaggta
     6661 atgtagtcgg ttctatttcc aataaatcct ttgctgctct catagtttct aaccaactag
     6721 attctttgtg ttgaccttga ggtgtaaatt cctcatatcc ttttttagca caatattcct
     6781 gtaatttagg cattaaatca tttccttctt tgtccttaat acttgtccac cacccgaaat
     6841 gattcaatcc ataatatcta acatcaaagt ctttacgaga atttaaacct aaaactctag
     6901 caaatatttc ctctaaacat actggcatat cacaaatatt aataattctt gaatttggtc
     6961 ttaatacacg acatgcttct gcaactatag ctgctggatt tgaataatta agcatccaag
     7021 catttggaga atatttttcc atataatcaa tcatttcgat tattggacct attgttctta
     7081 atccataggc aattccacct gccccacaag tttcttgacc aacaacccca tattttagtg
     7141 gaattttttc atcttgttca cgtaaagcat ataaaccaac acgaatttgt gccattacaa
     7201 aatcaatatc agtaaatgct tcttctggat ttgttgtata actaaattta atttctggat
     7261 ttttctctct aacaattatt gcacatgctt cagcaacttt tctttgtctc tcatcattat
     7321 tatcatataa tttaattgat tcaataggaa atttagcaac actttcaatt aacattgtta
     7381 caattccagc agtataagta ctacccccac cagcaattag tatcctctgt ctttttcttt
     7441 ccatctttcc ttctccttta taataaattt tcaaattctt ctcttacact tggtactgaa
     7501 agcccaataa taatctgaat agcttttcca ttatgcacta aaccatgtgc acctactgcc
     7561 ttaaaatctt cttctttaga aataattgag tcatctttca aggttaatct caacctcgta
     7621 gcacagtttg taacatctac aacattttct gctccaccaa ctaattttat gtattcagat
     7681 gcttgttgag aggctttact caatttattt cctttttcat ctttagtttc taagtattca
     7741 tttttagtat atagttttgt ttcttcacta tcttcctctc taccaggagt tttcatatca
     7801 aacttcttaa ttaagaaagt aaatacaaag aaccagataa atgaaaatat aatccctaca
     7861 acaacttgaa caacatatac cttccagtga tacattccaa gaggaagcca atttaataca
     7921 acccagttaa taattcctcc accaaaatca ccaacaactc ctagggcaaa cgaaatcgtt
     7981 tgcaagcatg catctaaaaa tgcatgtact aggaataaaa gtggtgctac aaataaaaat
     8041 gtaaattcta acggctcagt aattccagtt aatactgcag taagagtagc aggaatcatt
     8101 aaacctaata cttttttctt tttttctggc tttgcagtta catagaaagc accaaataca
     8161 cctaatgaac caaacacctt cgataatccg gataatgaaa atccgattgg atacatttcc
     8221 tttagagtct tagctgaagt ttgaaaatcc tgaatatgag tagcccaata tactgatgta
     8281 ccacctggaa caacaacact atcataacaa attggtgcat atacaaaatg atgtaatcca
     8341 gttggtatta aaatcttttg taagaatgca taacaccaaa ctcctatatt tccactattt
     8401 ataaaaaacg tttgcaaatg tctaataaca ttttgaatat gaggccaaat aaaacaagtt
     8461 aataatgcaa ctggtatcat aacaaagaaa caaattgcaa ctacaagtga tgagcctcta
     8521 aataatccaa taaaatctgg cagttcttta tcataaaatc tattatgaat ataaatgaca
     8581 atacctgata ttaacaaagc accaaccata ccagtatcta atgttttaat accagcaatg
     8641 cttacaagtc cacttccagc acttatttca gctgcgaaat caactccaaa gctttttccc
     8701 caaaaactta acatagaacc tacaaaataa ttaaaagtca aatacgttgc taaagcctcc
     8761 atacaagctc ttgcttgttg cttttttgca agtgctattg gtaaagaaat agcaaataat
     8821 aatggcaatt gattaaatac catccaagct ccactagata ttacacccca aaattggtac
     8881 caaatatttt ctggtgtagc aagtgaaccc ataattgact gattttgaaa tacaattgag
     8941 aatccaacca ttatcccagc aaaagcaaat aatagtgttg gtgtaaacat tgcccctcca
     9001 aaacgctgta ttttcttcat catataaatt actcccctcc tcatattttt agtttatctt
     9061 tggccttaga tatctattat tttaacaggt taataaatat atacaatctt tagggtacat
     9121 tcggagctta ttcacagtca aataagtttt tgcacatttt tatgttcaaa ttctcataaa
     9181 aaaaaagaat gactacaatt tgcaatcaat cccttctgta atcattctat gccttttata
     9241 aatactatat ttaataaata ataactcaat caatataaaa tataacattg tcaatttata
     9301 actagggtgc atttgtaaaa atgagatatt agtcgctgaa atataaattg attcatcact
     9361 taaatcagtc aatgtattat ttttaaactc tgtaattgaa attattttag ttcctattaa
     9421 ttttaatctt ctagcaatat taagtgtatt ctcattctca ccacttaggg atataataat
     9481 cataacatca tttttactga caatattgta aaaagaatca tcaaatgttc ttccctcaaa
     9541 atcatatatt atttcttgac atgataagaa cattctcttt atctgttttg caaccacatt
     9601 ttgaatatca cctgaaccat aagcgtatat tgttcttgat tcataaagaa gtttacacaa
     9661 tgcatcataa tctttatttt tttgatcttc aacatactta attattgaat tacatgcaat
     9721 atcaaatgac tgattttcta tgacatgaac cttttctgat tcccagctta ataatacttt
     9781 aaactcactg aaacctttta aatctaattt tttaacaaat cttgtaatag ttgcacgaga
     9841 cacattacat ttcatagcaa gttcatttat tgacatctta ctacaatgtt ttttatttgc
     9901 agttatatat ttccatatta ataaatctgt atcattcaat ttattataat ttaaaaaaat
     9961 catttcttct aatctcataa actattaccc cctttaatta aaaatcgtca taaatattat
    10021 accaatattc aacattaaat tacagcgtat tcttatattt actatttagc ccataaaatt
    10081 gactataaaa aatactgcta taattgatgt ttaaaaaaat gtagttttaa ttttattctt
    10141 ttatctaata attaatattt tcaagttcta gtgcttttct tatacaatta tcatctttaa
    10201 aatcagcagt ccttttacaa tcttttactt taaattctgg aatagtttta aattcctcat
    10261 cacatattcc attcttattt aaatacatac aacttgccat tctaactatt agaccactat
    10321 tttttaaatt aaacaataca gggtctatac accctccatc tccgccagta gtttcaccaa
    10381 ctaaagtcgc aaatttattt tctttacaaa acattgcaaa tgactctgat gatgaatata
    10441 tttttctatc aacaagtaag tatatattac ccttaaattt tgaatcatct gtagatttta
    10501 tagtatagga agtatcttca aaatcattaa aatcttttat aatttcttta ggtgcatttt
    10561 cctttacact tgctggtaaa ttttctacag gatttaattt tatatttctt gcatttgtat
    10621 atttttttac tatttcatta ttatttctat aaattctata accatttatt ttgacatcat
    10681 tttttatgag tttagatacg attccctgcc aatacctatc acttccacct aaattacctc
    10741 ttatatcaat aattaaagct ttatgttttt ctaaagtgtt aatataatct ccaattattt
    10801 ttaaatcatt atttatagag ccattctttg atgacattga tggtaaatac atatatccaa
    10861 tctgaccctt aataacatcc tttaaaatta attctttttt tacaaaaata tcatctggca
    10921 tttttatttt aagattcata gagtcatatc tatccacaac ttttttatca ttaagaaaat
    10981 cataccaatc attattagag tatgatttct taaaaagttc atatcttttc ttattatcta
    11041 ttacttctgt gtgtttatta tttaaatcag ataatataga tgacagttct tctataaatt
    11101 cttcatcatt ttcagtgttt tttattcgtt ttgtatattc atccttattt gctaaccaat
    11161 caatattatt aagcctctta tttacaccta gatatggata tcctgtcttt acaacattat
    11221 acacataatt aaagtcatcc accttttcct cttcagacaa ctgattcttt tgagaataag
    11281 tagaattacc taataatatt ataaatgcta aaataattac taaaaataat actttctttt
    11341 tcatgtaatt ctcatccact ttcagatttt atattttaat acattgtatc attattttat
    11401 tatcacctaa acagttaaaa gaaattcttg aagatatatt ccactattat tctctttaaa
    11461 aatttttatt tgtattttca aaattatttc atgaaagtta taaaataaca attatattca
    11521 aatatctatt ttactgctat atcctgaaag taaattctta tcagtgatat actaaaaaag
    11581 atatgctaaa aaatttatcc attataatga cttaaattta gatactttac tgatttttat
    11641 ttattagttg agaaagatat tgataattaa aaatattgat aactatagaa atgattattt
    11701 tttatagaaa ttttaaggat ataaactaat tatacttaag tctatatcct taatttatca
    11761 tgattatgag aatttttaca aatacttatc tttgtccgtt ttagaataaa aacaagaaca
    11821 tatcctgtta ttttattcaa acatccaagt ttaattacta cacaattact tattagaaat
    11881 tgtatctact tcatatatat atctgttaag tatagcttct agcatttctt gtcttccaga
    11941 cttattcttt ataactggca tttgcagtgc ataagattct aactctttaa atccaacctt
    12001 accttctact atatccttac ctataccttc actaaaactt gcatatcttt ctttagtaaa
    12061 gttttcaaat acctcatctt ctaataattt atgagctatt aataatccct ttgcaaatgt
    12121 atccattcct gcaatataag ctaggaataa gtcttctacc tcaaaagatg ctcttcttac
    12181 tttagagtca aaattaaacc ctcctggtgc tattccacct tgctttaaca cttcatacat
    12241 agcaagtgtt gcatcatata tatttgttgg aaattggtct gtatcccaac ctaataatag
    12301 gtctccttga tttgcatcta tactacctaa aacattgttt attcttgcta tatttaattc
    12361 atgttggaaa gtatgtcctg caagtgttgc atggtttgct tctatattca ttttgaagta
    12421 tttatccaga ttatactttc ttaaaaatcc taaaacagta gcagtatcaa aatcgtattg
    12481 atgtttagta ggctcttttg gcttaggctc tattaagaat tgtccagtga atcctatttc
    12541 cttagcatag tctacagcca tttgtaagaa tcttgcaaag ttatccattt caagttctgt
    12601 attagtgtta agtaaagttt cataaccttc tcttccaccc cagaatacaa aattttctcc
    12661 tcctaagtac ttagttattt ccatagcttt tttaacctga gctgctgcat atgcatatac
    12721 atctgcatta catgttgtag atgcaccatg aacaaatctt ggatttccaa acatattggc
    12781 agttccccat aataacttta tacctgtctt ttccatatta acttttatat actcaactat
    12841 ttcatctaat atcttatttg tctcttctaa atctcttcct tctggtgcta tatctctgtc
    12901 atgaaaacag aaatactcaa ttcccatctt agacataaat tcaaatcctg catcaacttt
    12961 aatctttgct atctcaattg ggtctgttat attattccaa ggtctctcta ctgtgcctac
    13021 tccaaatggg tcattaccct ctccacataa agtatgccaa tagctcattg caaatcttaa
    13081 gtgttctttc atagtcttat tacctaccac ttgttcagga ttgtagtatc taaatgctaa
    13141 ttcattgtca gattttactc cttcaaattt tatctgtcct attcctttaa atatttcact
    13201 catttcatgt cctcctaata ttttacacat ataatatatg tagtatgttt tcttaattta
    13261 aaactatttt ctatctttat ttttcttttt aagctaattt aattatttat ttttgcatta
    13321 actacttcat ctaataactt aaagtcatct ttcaaagatt tgtatattct tttatacaat
    13381 ctatgatata tattgtatgt atctacattt tcacttattg gttctaattt agttgaagtt
    13441 tttattatag catcacaagc tttttgtata ttttcatata ttccagctcc tactaatgca
    13501 agaattgcaa ctccaagtgc tggtccttca cttgaattta tagtatatac atcttgatta
    13561 aacatatctg cttgcatctg tctccatact ttactttttc cacctccacc tgaggctctt
    13621 acttcattaa cgtctatatt aagattctct attatatcca tacagtcttt aagactatat
    13681 gaaacacctt ccattattga ccttgcaatt tcttttttag aatggattgg agataatcca
    13741 aagaacacac ctcttgcata agggtcaaga tgaggagttc tttctcccat catatatggt
    13801 aagtaaagca atccattaca tcctgttggt actttactta cttgattatt tataatctca
    13861 tatacatctt cacctaaaga atttgaaact tccacttcac tttgacagaa attatcttta
    13921 aaccatttta atgacaagcc agctccttga gtaactccca taacatgcca tgtatttggt
    13981 attgcatgac agaaagtatg aactctacct tctttatcaa tttttggctc ttttgtataa
    14041 gcaaatacta cacctgatgt acctatagta gatgaaacta ctccatcttt aactattcca
    14101 tttcctatag ctcctgctgc ttggtctcca gctcctccta ccactattgt tccttcttta
    14161 agccctgtca aacttgatac atctctagtc acataacctg taatctcttg tgattcataa
    14221 actttaccta aaagattttt atctatattt aacttttcta gtacttcatc gctccaatct
    14281 ctttttctta tatcgagcaa ctgcatacca cttgcatctg atacctctgt agcatattca
    14341 cctgtcaatt tgtatcttat ataatctttt ggaagaagta ttttattagt atttttatat
    14401 atatctggtt cattgtttct aacccataat atctttgatg ctgtaaatcc tgtaagtgct
    14461 ggattagcag ttatttcaat cagtctttct tcacctacta aatcagttat ctcctcacac
    14521 tcttttactg ttcgttggtc acaccatatt atagagtttc tcaatgtttt accttcattg
    14581 tccagcataa ctagaccatg catctgccct gataatccaa ttccttttat acattcactg
    14641 cttatatttg aactttgaac tacttctctt atagattcga ctacagcttt ccaccaatca
    14701 tttgcatcct gctctgccca acctgatttc tcttgtataa gaggatattc atatgtacaa
    14761 ctttttatag tatttcctag tttatcaaat aaaactgttt tcgtacctga tgtaccaata
    14821 tccacaccta atacatactc caacttcatc cctccttgaa tgccacttca taactatcgt
    14881 tttcctatcc taattttttt atattttagg tatattttac ttgctttatt tttgttagta
    14941 tattaaacta acttatgaac taataataaa atatttaatt atctttgtca atgcaaaata
    15001 accttttatt tttgtgtaaa aagtattaca atattatttt aaggaggaat atcaaattgg
    15061 ttactgataa atacactatt cgagaaatga atgaaagatt ggtacttgaa caaattataa
    15121 aaaatggtcc tatttctagg gcaagtatag cttctactat aggattgaat aaagcaacta
    15181 tatccgctat taccaaaaaa cttattgatg aatctctagt tcatgaaata ggaattggaa
    15241 atagtactca tagtggcgga agaaaaccca tattattagt atttaataaa tgtgcaggga
    15301 tttctttaag tatggacatt ggatacgact atatattttc ttctctatct tacttagatg
    15361 gaactattat aaattctaag aaattaacag atatacaagt aagtaaagac aatgttatac
    15421 aattgataga tgaaattata aatagttata acatatccaa aatagacact ccttataagg
    15481 taattggtct tactttggct atacatggga ttacttgtga aaataaagta ttgtttactc
    15541 catattacaa tcttaatgag attgacttat atagcatcct tagcaaaaag tatgactttc
    15601 ctattcatat agaaaatgaa gcaaacttaa cagctttagc tgagaatact ttttcaactg
    15661 tacataactc actacttagt ctcagtattc acagtggatt tggttcaggc attataataa
    15721 ataataaatt atatagtggc agaaatggta tgagtggaga aattggtcac actatcatca
    15781 tgcctaacgg taaactatgt ccttgtggaa atcgtggctg tttagaacag tattgttcag
    15841 aaaaaaaggt atttgaacag ctttcttctt tagaaaatat tcctaaaata gattctgata
    15901 tagtaaagca actatattat gaagataacc ataatgccaa aaaagttatt catgagtttt
    15961 gtagttactt aacaatagct ataaataatg ctattacaac ttatgctcct gaaataatat
    16021 atttaaatag ccaaataatt agtgatatcc ctgagatatt acagattaca aaagatatgt
    16081 tagttagctc ttttaacaaa ggaataaata tagaaatttc atcattaggg tctgaggcat
    16141 ctctatatgg tggctctgct gtaaatatca agagcttttt gaatatacaa aatttaactt
    16201 taattaatga aatataaggg ttatgactat atacagccat aaccctttaa attatacatt
    16261 ctaaaattag atttattata tagtttatgc aagattagct atatttttta aaaattgtat
    16321 atcaattaag tattaacata agtatttcgt tttaaatatt aatataatct tcctactcat
    16381 catcagataa taaatttgca ttttgttcaa ccatagaaat attatcttta cctattttct
    16441 ctaattttgc tgccaattcc tttgtatcta aaaaagctga ttcaataacc atacctaaat
    16501 ttagccctga aataatttta attcgactgt cattattggc tactaataaa gcagcattgc
    16561 atggagttcc acctagtaaa tctgccatta taattatttc tgaactttcc ttgtacactt
    16621 ctaaagcttt ttttaatttt tctattgttc cttctattcc atcatcttgt ttcatattta
    16681 cagtaggata atctatagaa tcccctatta tcatttttgc agaatttaag acttcttcag
    16741 ccatattgcc atgactcatc aaaataatac tattcaattt aatacacctc tttaaatcta
    16801 taagatgtct acaagataag ttttcttatc atcaggcgtc attatagttg taatttcaat
    16861 tccttcatct aaaagtcttt taaatgcttc tttctcttct tcagtaacac tgatattagg
    16921 tcttagaact gttgtatctt ttctacttga catatttcct acatttatat tatgtatttt
    16981 aaatcctgcc tctacaaatg gaataaggtc cacaggagac ttaacaatta ataatacatt
    17041 ttgtccttca tactttcctg cagtaatatt ttttatagca gtctcttctg taattataga
    17101 tgacgctata cctgcaggtg tagccattct taaaacttgt ttttgaacag aattgttagc
    17161 tactgcatca tttactacca ttaatcttgt aacacccaat ttattactcc acatagttgc
    17221 aacttgacca tggattaaac ggtcatctat acgaatattt ttaatacctt tcatttccat
    17281 atctaatatc atcctttcct tattttaaaa tacctaatgc atataaaatt attgaaacaa
    17341 ccattactcc taaaattaag tatataggtt ttatattctt tttaagagcc caataaacaa
    17401 tcacaactaa aattgcagga actaacccag gcataatttg atctaatatt tcttgacctt
    17461 tcattgtaaa atcaccttgt tgaaagttca aagcaacatt agcttttaca actgaaggta
    17521 ttaatgcacc tacaaccatt aaacctaaaa tagatgtact ctcagttaaa atctttaaac
    17581 ttttttcaat tgaagatatt agcttagtac ctgatgtata accagcatta aatagtggta
    17641 tacttaataa tttcaatgct acactcacta caatccacag taagattcca aatggacttc
    17701 catctatcgc catattagct gctatagctc caaaaattgt ccacggaata acgacaaata
    17761 aactatctcc taaacctgct actggtccca tcaagcctgt tttaattgaa gcaacagcct
    17821 cttcagtctc ttcacttgat aactcctcca ttgctacatt aatacccaaa atataagggg
    17881 caacccaagg attagtgtta aaaaattgta aatgatttaa tacagcactt tgtaactttt
    17941 ctggattatc tttgtatact acttttaaaa aaggtagtac tgcataacag tatgccaagg
    18001 cctgcatctt ctcataattt gaagaaacag aactagtaaa aaaccagcgc cataagacac
    18061 tttttttcac cttttcattt gatttattta atgatacctt atcactcatc ttcgtccata
    18121 cctcctacta cagtatattg ttgttcttga ttttccaaat tctttttgta aattataagt
    18181 gcaattgctc caccaattaa agctacacct aataatggta ctttcatata tactgctaaa
    18241 acaaaaccaa ttactaagta actaaaatac tccttagcag gaagatatct aagtaataat
    18301 cctatcccta ctgctggaag tagtcctcca gctactgtta aaccacctgt taaccattca
    18361 ggtgtatatt tcaaaatggt attaattaaa gaaggtccaa atattacaga tagagcaact
    18421 ggaatacctg ttgtagctgc tgttaatagt actcctaaca tttgcatata ggttatattt
    18481 ttataatttc cttttttagc tccatcttct gctttatgaa ctagccatat accaatagta
    18541 tttctaagaa catctaattg taccatcaat aaagcaactg gaatacctat agagattcct
    18601 acagatgctt cttgcttagt cgtaatagtt ataaatgtgg ctactatact tgctgtttga
    18661 taatcaggta ttgatgctcc accaaagtta ctaataccta atgataatag ctgtaatgta
    18721 cctccaatat ataaccctgt atttatatct cctaagataa gaccagtaat aaaacctgct
    18781 gttactggtt gatatgttcc aaacattgtt gaattttttt catagtttat aaaaaatcca
    18841 tatagtacga ttaataataa ttgccataat tgtggctcca ttatgatttc acctctccca
    18901 aaatattttt tagttcttta aaattataac tggaatcttg ctttaggaaa ataggaattt
    18961 cttccagtgg gcattcaact tcataagttt tcccaccttc ataactaact ccactaaata
    19021 tattttccca tgtatgtcct tttggtaaat atacttttct acttctatct ttataattca
    19081 ttattggagc tactaaaatt tcatctccaa gcatatatgt attatccacc tcccaagttt
    19141 tatcatcttc aggaaattca taaaataaag ttctcataac tggtgttcca tcttcatgcg
    19201 cttctttcat cagttttttt aaataagttt ttaagctctc tctaattttt atgaattttg
    19261 taagaattac ttcaacttct tcaccataag accatatttc attaggagct ccagttacca
    19321 ttgagcctcc acctttattt gataatggtt tactatgagg caatctatct ccatgcattc
    19381 tcaaaattgg tgaaaaagta gcatattgga accatcttac cataagttct ctaaattctg
    19441 ggtctttagg atttcctcca tggaaacctc caatatcagt agtccaccat ggtatacctg
    19501 ccagtcccat atttaaccct gtatttactt gattattaaa agcttcaaaa ctagaatcaa
    19561 tgtcaccaga ccatactaaa gctccatact tttgagctcc tgcccaagct cctcttacaa
    19621 gagttacaac agactctatg tcattatgta aaccatcata aatcattttt aaataatcaa
    19681 taggataaat attaccacaa cttaaaacat ttcctttttt atatctatag ttatcaaaat
    19741 catacacaga atagccaggt tcagctacat ctaaccaata atagtcaatc ccaagttgtt
    19801 tataattttt atctattcta tcccaaacat attctcttgc attttcattt gtcatatcta
    19861 caaagactgt atttccttga atttgcattg tcattctaac accacgatta acattgacta
    19921 aatagccatt ttctaaataa tcattgtagt tttctgcatc actttgaact gtaggccata
    19981 cagatactat tggttctact gaatactctt ctttcaattt tttaactaat tctttaggat
    20041 ttttccaata atctaagtca aatttatatt caccttgctt aggccaatga aaatagtcaa
    20101 ttgcaattga tgaaagctta attcctcttt tagaatattc cttcacaaca tctaaaactt
    20161 cttctgaagt tctatatctt agtttacttt gccatagtcc taaaaggttt tcaggcatca
    20221 taggaacagt tccagtaact tgagagtaat ttttatttaa ctcttttggg ctttctccac
    20281 aagtaatcca ataatctata aaatttgtag aaaacatctt ccattctgtc atatttttag
    20341 caaaagatac ttttccaatt cccggattat tccataaaag tccataacct agactagaaa
    20401 tataaaaagg aactgatatt tgagaatttc tttgggctaa ctccaaaaca gtattcttta
    20461 aatctaaaaa tttatgttga tattgtccca ttccaaaaat cttttcagaa ggctcagatt
    20521 caaatctagt agttacttca aattctccac tatagcttgg tcgatattct cttgatttta
    20581 actttagagt agagttaaag tctttggtaa tctcaattgt ccctacatct tctccaccat
    20641 catcatgttt tacagctctt aatcgaatat attctttcaa taatatttca tttttatcat
    20701 tataaaaagt aattctatct ctatggtcta agacagcttt tatctttcca tttttaatga
    20761 ttgcacttcc atcttcattt ttattaattg taatatcatc tttatttaac ttaggttttt
    20821 cgttaagagc atagttttca tcaacaaaat tttggtctac aaacgaccta atacgtaaag
    20881 agttttttcc ccatgcttct attcgcaata gttcattgtc aaatcttttt tcaatatagt
    20941 cttcagatat ttttatcacg gctacctcct aaatcaatgg tataatattc tatctttgtg
    21001 taacttaagt taaataatgt cgtttagtat cgttatttat aaattaggtt ttatatataa
    21061 ttcatttgta cattaaacta acttaccaat aatatacttt atttgacttt gtttgtcaat
    21121 cgctttccgg attttagtaa aattttctaa tatattgctg gaaagttgtt cttaatatct
    21181 tttatttata cttttaatat ctgtgatgat acctatatac atctagtttt tttaaaacac
    21241 atacaggaat aacatttgcc tattatgtag ataatatcgg acttgaaaat atgtatagt
//
LOCUS       DAELTA010000059        21191 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.059, whole genome shotgun sequence.
ACCESSION   DAELTA010000059 DAELTA010000000
VERSION     DAELTA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.059"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            167..1177
                     /locus_tag="KPG13_001834"
     CDS             167..1177
                     /locus_tag="KPG13_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0985567.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1313..1801
                     /locus_tag="KPG13_001835"
     CDS             1313..1801
                     /locus_tag="KPG13_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0985568.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1977..3905
                     /locus_tag="KPG13_001836"
     CDS             1977..3905
                     /locus_tag="KPG13_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0985569.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4082..4492
                     /locus_tag="KPG13_001837"
     CDS             4082..4492
                     /locus_tag="KPG13_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG0985570.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4514..4816
                     /locus_tag="KPG13_001838"
     CDS             4514..4816
                     /locus_tag="KPG13_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG0985571.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5033..5725)
                     /locus_tag="KPG13_001839"
     CDS             complement(5033..5725)
                     /locus_tag="KPG13_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0985572.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5751..6938)
                     /locus_tag="KPG13_001840"
     CDS             complement(5751..6938)
                     /locus_tag="KPG13_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0985573.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7274..9151
                     /locus_tag="KPG13_001841"
     CDS             7274..9151
                     /locus_tag="KPG13_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0985574.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9508..10914
                     /locus_tag="KPG13_001842"
     CDS             9508..10914
                     /locus_tag="KPG13_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG0985575.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10942..11790
                     /locus_tag="KPG13_001843"
     CDS             10942..11790
                     /locus_tag="KPG13_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0985576.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12451..13143
                     /locus_tag="KPG13_001844"
     CDS             12451..13143
                     /locus_tag="KPG13_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985577.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13140..14075
                     /locus_tag="KPG13_001845"
     CDS             13140..14075
                     /locus_tag="KPG13_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985578.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            14223..15146
                     /locus_tag="KPG13_001846"
     CDS             14223..15146
                     /locus_tag="KPG13_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985579.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15130..15900
                     /locus_tag="KPG13_001847"
     CDS             15130..15900
                     /locus_tag="KPG13_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985580.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15910..16692
                     /locus_tag="KPG13_001848"
     CDS             15910..16692
                     /locus_tag="KPG13_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985581.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            17069..17605
                     /locus_tag="KPG13_001849"
     CDS             17069..17605
                     /locus_tag="KPG13_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0985582.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17637..18026
                     /locus_tag="KPG13_001850"
     CDS             17637..18026
                     /locus_tag="KPG13_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG0985583.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            18063..18449
                     /locus_tag="KPG13_001851"
     CDS             18063..18449
                     /locus_tag="KPG13_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG0985584.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18576..20018
                     /locus_tag="KPG13_001852"
     CDS             18576..20018
                     /locus_tag="KPG13_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG0985585.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20315..21130
                     /locus_tag="KPG13_001853"
     CDS             20315..21130
                     /locus_tag="KPG13_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0985586.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7819 a         2474 c         3900 g         6998 t
ORIGIN      
        1 attttgggta ttgtatactg gaattagagt aattaattta ttttttaaca tacaaatgat
       61 tttatgaaaa aaaacaagac tcaaaaattt ttatattctt tgatagaata aaattgagag
      121 taaatttatt gaattttata aaatttataa ggggtgagtc taagatatgc atgaagataa
      181 aaatgatata tttgaatcaa tagatactga ggattttgat aataaaacag aagaagaatg
      241 tttagagcta gtaagaaaaa atggattgaa tttaatatac ataaaaaatc aaacagaaga
      301 gatatgtcta gaggctgtta gacaaaattg taatgcaata aaatgtgttc agaatcagac
      361 agaaaaaata tgtatagaag cagttaaaca agattggaga atgttagaat ttgtggaaga
      421 gccaacagaa gaaatttgca tgttggctat taaccaagat ggaacagcat taaaatatat
      481 agaaaatcag acagaggagt tatgcttaag agccgttgaa aaaaatggtg cagctttgga
      541 gtatgtaaaa gaacaaacag aagaaatatg tatagaagca gttagaaaca gcgaatttgg
      601 attggcaaga gtgaaaaatc aaacagaaaa aatatgtatg gaagcagtta agcattgtag
      661 ctataatcta aaatgggtag aaaatcaaac agaagaaata tgtatggaag cagtgagaca
      721 aaatgggctt gatttgaaat ttgtaaaaaa tcaaacagaa acaatctgtt taagagcagt
      781 aagacaaaat ggaatggcat tagaatttgt aaaggaacag actgttggta tatgcttaaa
      841 ggcagtaaga caatatggaa tggcattaaa atttgtaaag gaacaaacag aagaaatttg
      901 tacagaagct ataaaacaag ataaaagagc acttagtttt gtaaaaggag ataaggaaaa
      961 atataaggct ctatatgata ataatgaacc atttgcaaag agatatgtta gaaatgtaat
     1021 agaaaaagaa aacagagcat tgattaaaaa aggagaagaa aatgcaaaaa tcaaaattgc
     1081 aggaaaactt tatgatagag gcatgtcttt tgaagatata tcagatatag ttgagattga
     1141 aataagcaag ttaaaagtga gtttaggtgt tttttaaagt aataaatgta ggtgtttttt
     1201 atgggaaata gttaataaaa aaataatcga ctagctcttt gtaggacagc aaaattagtg
     1261 atataatttg atgtataata aattattttt attagataag ggggaattaa agttgaagat
     1321 tacagtatta taccaaacaa gaagtggaaa cacagaaagg gtcgctaagc tgatagaaga
     1381 gggcgctaaa aaggtggatg gagtagatgt aaagcttatg agacttgata atattgattt
     1441 agattattta aatgaaagta aagcagtaat atttggaaca cctacttatt tagcaaatac
     1501 aacttgggaa gtaaaaaaat ggtttgatga agattcaaaa aaagttaatt tagctggaaa
     1561 attaggagct gtttttgcta catgtgatta tatatgtgga ggtccagatg ttgcgatact
     1621 tactatagta ggacatttga tggttaaagg gatgcttgtg tattctggag gtggttcttt
     1681 aggaaagcca tttatacatt taggacatgt acattcaatt gaaggtccag agttacaaga
     1741 tgagaaggct ataatatttg gtgaaagaat tgctactaag gcaaaagaac tatttgctta
     1801 atttctatat gcatagtaat gattagagta aatttagggt tttaatggag atttgaagta
     1861 aaagttataa aataaaaaat tttccataaa attactcaag atgaattata atataattat
     1921 aaataaatta tattattaaa atatttaaaa tcaaacgtca atagcgaggg aagtttatgg
     1981 tatggcatat agaatatgag attttttctg caattatagt tattttttta atggtatact
     2041 tttttagaag taaatttata cctactttac aaaacaaaat atattgtgca cttttaatat
     2101 tctcattttt atttataatt tcaaatattt taggttcatt ttgtctaaat aatatagaca
     2161 aaattccaat ttttattact tttttattga atcagatata cttattgtta ttgccaatac
     2221 cagcagcact tgtatcattt tatgttatgg ctattattta tcaagatata aggtatatga
     2281 aaaaaagatt attattttta tctataccac ttataatttg tataatctta tctattacaa
     2341 atccttttac gcatattcta tttggattgt caaaagaaac aggttatata cgaggttgtg
     2401 gatatgttgc aacttttgca tcttttttct tttatgcaat atattcagca tttttatctt
     2461 ttaaatatag aaaagcaatg catgaatcaa aaatatgggc tattagaggt tttcttatag
     2521 tttcaattgt ggcaattatc ttacaggcaa tttttttaca gtatcttctt acaggaactg
     2581 catgtgcatg tagtttactt ttgacatatc tttctatgca gaatcgaggt cttattattg
     2641 atgaccttac tggtgtactc aatagacaat cttttattca agagctagat ttgaatatca
     2701 atactgaaca atcaggattt attattacaa ttgcgcttga tgattttaag tttatgaatg
     2761 aaacttttgg aacaaaaaat ggagatattg cactaaaaga agttggaaaa tatttgattc
     2821 agatacatga tgtaaaccat gtatatagaa tagggggaga tatattttct attgttgtca
     2881 atagtcaatt aggtatagaa ccagatgata ttatcagaaa gatagaagaa agatttaaaa
     2941 agccttggct agtagaagag ataagtttta atttagcaac tagtattgct gttgtttatt
     3001 acccagaaaa tgcagaaacg acagaagatg taattgttgc tatagatttc tctatacatg
     3061 aagcaaaacg ctctgagtca aggcgagttg tttatgcaga tgcgtctata agtgaaaaga
     3121 taaagaagaa acatgttatt aaaaattgtt taaaagaagc attaaaaaat gatgggtttg
     3181 aagtgtacta ccaacctata ttttctaata gcaggggcag gtttacttca gcagaggcat
     3241 tacttaggct agaacacaaa gagttaggtt ttatacctcc tgatgaattt attccaattg
     3301 cagaacaaac aggattgatt aactcaattg gattagtagt ctttgagaag gtatgtagat
     3361 ttatagctag tgatgagttt gaagatttaa aattggataa tatagctgtg aatctttctg
     3421 tagtacaatg tatgcaaaaa aacttagctg atgatttact ttatcttatg aataaatata
     3481 atgtatcacc aactaaattt aaacttgaaa ttactgagac tgtggctatg ggctctttta
     3541 atataattaa agaaacaatg gaaagattaa ttgatttagg tgttaagttt gcacttgatg
     3601 attttggaat tgcttattct ggagttacta atatgctttc tttaccattt tcagtaatta
     3661 agttagataa gagtttgatt tggtctatga atgaagatag taggcataaa cttacagttg
     3721 agacgattat agcacttata aataaattga atatgaaggc tgtagcagaa ggtgttgaaa
     3781 ccattaagtg tgctgaagat ttaatagcta tggagtgtga atatttgcaa gggtattatt
     3841 tttctaaacc agttccagaa gatgttttta aaaatttgtt gaaagaaaat acttttacaa
     3901 gttaacaccc tttatttttt taattacaac tttgttcatt gacactgcca gcgatatatt
     3961 ataaaattaa cacaataata aatattaaat ttattataca tattattaat attaattaat
     4021 ggagtaaaga tttattatat attttattta attatcctaa agtgagaaag gaagatgtga
     4081 catgaataaa aagaaaatat tagtgccaat tgatgggaca gaaagaagta tgcattcttt
     4141 ggaatttata aaaggaatat ttaaaaaaga tgaagttgaa gtagaaataa tgaatgtaaa
     4201 agaactagta tttattgatg gcatatccct ggctgaagag ataaaaaatt ctgaaaattt
     4261 aggaagaaga atcttggata aagcagcaga gataatggga gagtatgatg ttaaagtaca
     4321 ttttactttt ggatatccag gagatgagat aattagaaaa gcaaaagaag ataatgtaga
     4381 ttttatagtt atgacaaaat ctactaaaaa aggtcttaca agaatgatag gttcagtaac
     4441 agcaagtgta gttaaacaag caaaatgtat agtgatgatt gtaccggagt agaattagaa
     4501 aggtatttga aatatgaata aaaagatagt agcagtttgt gaaagtgaaa gaagtttaaa
     4561 ttctcttaag acagctgcaa atgatttagg atttgattta gtttatgaaa ttcaagaaaa
     4621 tgataatata aaaaatgaac tttctattta tgatatagaa gatgcatcca tagtgttatt
     4681 tgttacaagt gattctatag aaagcataga gaagattgat agatttatag atagagaatt
     4741 ttatgaagtt gatccaaagt atataataga ggatgcaaaa tctatagtaa gtgaaatttt
     4801 aatagactta aattaatcgt gggtgtcgca ctaagaatag agcgacgcct ttttatatat
     4861 tttacacaag aaataaagag gttgtatcaa aatatagata agagtctttt tcattgttga
     4921 aatgatgaaa atatatttga tttcatacaa aaaagagtac ctcataacta aaaagttcac
     4981 tttgagacac tccattttta tatgttagtt gcatttaagg taaaatagaa gtttaatcat
     5041 gttgaaataa ctgaattaca tcttctaaat gattgtacat tgatgtataa cctaagtaag
     5101 agtctttact atcaaatgca atcattatct tgttatggtg gctaatcata tccttgagag
     5161 aatttctata tttaaaacca aagtattggt tagaagaaac tgttttaggt attaactcat
     5221 aatattttat aaaaattgga ttatgagaag ctaaaattat ctctttgtga aattgcaagt
     5281 cagcatcata acattcttct atcaaaccat gagaaacctt atccaattcc tctattgact
     5341 ttaggtaagt atattttatt ttttgaagtt ctttagatgt agcatttaaa attgctagtt
     5401 tagtagcttc gctttcgata agttgtcttg ctcttaaaga ttctacataa aattcttgta
     5461 tgttatgatt tttatagatt tcaccatcat caaataggtc aactttttta aatccttcaa
     5521 ctataggact tgaataaggc acagctctaa gtattccttc cttacagagt atatttatgg
     5581 catttcttac aggtattcta cttatgttaa ataggtcagc aatctttctc tcacttggta
     5641 atttactatt aattttaaaa gttccattta gaatgtttaa tttaatataa tcaataattt
     5701 tttcagtggc atttaagctt ttcataaaaa ctcctaaaca tataattttt ttatgattgt
     5761 attatatcat agttaagagt taaatctact tttttataat atgtataaga caggatagag
     5821 gccaaaatag ctataatagc caagataaag tatccatatt gtattgaagt atattccgat
     5881 attttaccaa ctatcattgg aaatacagac attcctatag cataaattgc ttgaaaaaaa
     5941 cccattgcag tagatttctg atgttttggt acatttttca tagactcgct agtaaggtaa
     6001 gaaaggagta tacctgtaga tatacctggt agtacttgta gtacaaatat tatattaata
     6061 ttttttattg ttggaactaa aaagcaatag gctgaaacta aaataaaaac taaaggaatc
     6121 caaaattttg gtccatactt agtacaaaat ttagtagaag caaagccaga agaagcaaca
     6181 gcacacacca tgtaaaatat agaagataga cctatgaggg tagaactaga acctaagttt
     6241 ttaagaattt gagtagtaaa tgacattgtt gtagacattt gaataccctg ttgaattaat
     6301 gccataaagg aaaaaaatac aagtctttta tccttgcaga tagataaata ttttttaatt
     6361 gatatttttt caggtatttt tttagaatta tgatttttta tatttaaggc tagaatcgtt
     6421 ccaataactg aagacaatac actcatgata cataaggctg tcattcctaa tttttcataa
     6481 aataaagtac tcataacaaa tccaatcaac attcctagat tgtttaccaa tattatcgta
     6541 cttgtagctt tttgttgatg ttctgtagaa aaaaagttgg tatagtgaac catgtaagaa
     6601 atccaagttg atgatgatat tccagataaa atgttagcta taagaaaacc attgcctcca
     6661 ggagaaaaga ctcttataag agatgctaaa ccagcaaaga aaattccaga cagtataaat
     6721 attttatgtt tatttatgga atcagcaaat actccaacag gaagccttaa taaacattgt
     6781 gatattccat atgcacctat tattatacca atcatatttg tagatacatt acatgcactt
     6841 aaatatggat tttggaaagg tatatataca tattgtgaaa accagaaaaa tgtaacaata
     6901 gaaagtaaaa gtaatttttg attagatatt tttttcatat tatcctccat gtggtatttt
     6961 tataatgcaa ataatatacc atttagattt tttaatcaag tactttctct attaaaaaaa
     7021 atttaaatat ataattatta tttacaaaaa aattatactt tatagttgaa ttaagaaaac
     7081 gcttatgata ctataaagtt aacaaatttg aatatataac agtagacgtg ttactggtaa
     7141 tgcaggcaag atcgaatcaa tttttataga ttatctatag agtactatgg gtataatcta
     7201 tttatttggt tcgatttttt tgtttaattg gccataaaac aaaaagccta tagttgaaaa
     7261 tggaggattt tttatggatt ataaaaatat agctcaagaa attctactaa atgtaggagg
     7321 aaaagagaat gtaaatgaag tgactcattg tatgacaaga cttagattta aagtcaaaag
     7381 cgcttctaaa gtgaataagg ataaattatc taaaactgaa ggagtaataa ctgttgttga
     7441 gagtatgggt caaatacaag ttgtaattgg aaataaagta aaaaaagtat atgatgaggt
     7501 cataaaaatt gtacctcaat caaataacat aggtaaaaag aatgaaagtc aagaaaagca
     7561 aggtataatt aattctatat tatcagcagt agctggtata tttacaccta caatacctgc
     7621 tatagcaggg gttggtatga ttaaaggtat tttatcagtt cttgctatgt attatatgaa
     7681 taaaaatgga gtagatataa aagaaacgca aagctatatt atcttaaatg ctatggctga
     7741 ttctatattc tattttatgc ctattatact aggatatact gcggctaaag tatttaatgc
     7801 aaataaaata atatctatgg tattaggagc tacactttgt tatccaactt ttactgcttt
     7861 gatggcaggt gaagaatctg ttagattttt aggattggca gtaacaaaag ctacgtatac
     7921 atcatctgtt attccaataa ttattgctat atgggctctt tcatacgttg aaaaagtatt
     7981 agaaaagtat atacctgagg taattaaaat aataatggtt ccaactttat cattggtagt
     8041 aatgttacct gcaacattat ttttatttgg accaatcgga atttatatag gaaatgtcat
     8101 aaacttttct tataagtata tttatgaatt aagcccagca ttgtgtggag catttgtagg
     8161 tgggttatgg tgtgtgttag ttatatttgg tgctcataga gcattattac caataggtat
     8221 aagtgatgtt gctcaaacag gaagacaaaa tttattggcg tttgcaggag ctgcaaactt
     8281 ttcacaagca ggggctgctc taggagtatt ctttaaaact aaaaatcaag gactaaaaac
     8341 aatttctatg tctgcaacta taacagcttt atttggtata actgaacctg caatatacgg
     8401 tgccaatctt agattaaaaa aacctatggt atgtgccgta atatgtggtg catttggagg
     8461 tgcgataatg ggaatgggtg gagcttatgg taatgcattt gcaaaccaag gtgttttaac
     8521 tatacctgta tatgctgaag caggtgcatt aggtttctta agttatctag gtggttgtgc
     8581 tatagcattt tttggttcag ctatttcaac ttatctagtt ggatttgaag atttggaaga
     8641 gtcttctaat aaggaaaatt catctataaa agtagaaacg aaggatggtg ttattgatat
     8701 aacttcacct gtagagggcg aatgtataga attgtcagaa gtaaaggatg atgtatttgc
     8761 ttcaaaagct atgggagaag gtatagctgt gttacctaca aaaggagtaa taacagcccc
     8821 tactgattgt gaagtagctt ctttatttcc aacattacat gcaataggat taaaattaga
     8881 taatggagca gaaatgttaa tacatgttgg aatcaataca gtagagttaa atggtaaata
     8941 ctttacaaaa catgttaatc aaggagattt agtgaaaaaa ggtgataaac ttatatcgtt
     9001 tgatatagat aaaataaaaa aggctggtta tgatgtaact acgccagtta tagtaaacaa
     9061 tacatttgat tttgggcaag tagtttcttg taaatctagt tatgtttcaa ccaatgataa
     9121 tataatttca ttagtattag ctggtaatta agttaatttg actagagata agtaaattga
     9181 tttaaaagga aataagtaaa tcaacaatta aatacatcta aaagtaagtt tattgcaatg
     9241 aattgttaaa gaaaataatt aatatggagt aaatctattg caatgaagta acaaagaaaa
     9301 taattaatat agagtaagtt tattgtaatg aattgttaaa gaaaagagtt aatatagagt
     9361 aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc tatctcaatg
     9421 aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca acaaagaaaa
     9481 taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga ctttttatgg
     9541 ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa taaaggaaaa
     9601 accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac taaagtcgca
     9661 tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt agggatgaaa
     9721 acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg agagataaac
     9781 cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg tgatattgag
     9841 ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa atataatggt
     9901 tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg ctttaagcat
     9961 tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat tgttagagtg
    10021 gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa aatgcgtgct
    10081 cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg tcatgagatg
    10141 ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc tcttacttct
    10201 aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta ctatatgctt
    10261 gatatgcatc attatggaga atatccaggg tattatatga agtatcttga aaaaagaaat
    10321 atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa aatggatttt
    10381 atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga ttcacaacat
    10441 ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt taaaatgtca
    10501 atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc aggtcttaga
    10561 gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac agaaaatggt
    10621 ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg tatagactat
    10681 ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga gatgattgga
    10741 tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa taagcgttat
    10801 ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag aatcaagaaa
    10861 gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa ataaacataa
    10921 atatatattg agggttttag tatgtacatt acaaaggtat taaataattc gttgctactt
    10981 gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg ccataattat
    11041 aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct tcatgatgaa
    11101 aatataaaaa agagtattat acaattggca acagaaatag atgcagaata ttttggtata
    11161 gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat gaaccatata
    11221 tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga aggtataatg
    11281 attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta tgatatagga
    11341 aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga agaagagatt
    11401 ggtaatatag caggccattt tataaatgca caacaagata atccttatag tgatagaaat
    11461 aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca cttttctatt
    11521 gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa ggccttttca
    11581 caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata tgagcaagtt
    11641 caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta tatgtataag
    11701 gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca tattcataag
    11761 attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta atattataaa
    11821 aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg gggttgtctt
    11881 aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct ttatatcatt
    11941 acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta catttctcaa
    12001 catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga gttaacccta
    12061 aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt tctggaataa
    12121 ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct tcaactctgt
    12181 atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata cgcttgattt
    12241 tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca ttacacttga
    12301 agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt tcattgagga
    12361 acatattcta agtgatgtca agatactaca atatgactaa attcctagtg ataaaaaagt
    12421 ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat agatgatgat
    12481 aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga agcaaactat
    12541 tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca gttagtagtt
    12601 ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat acgaaaatat
    12661 agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa agtgcatgga
    12721 ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga atttactgct
    12781 cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga ggagccacag
    12841 caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac taccaatggc
    12901 gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc aaggcatcag
    12961 ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc atatgtatat
    13021 gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga actagaccca
    13081 tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa cacggaggta
    13141 tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt ggtctacttt
    13201 ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta aaagatatag
    13261 aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta attaagccat
    13321 gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat tatgaaaata
    13381 agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca agtttatctc
    13441 atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta cacagtggta
    13501 tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga gcctacgacc
    13561 tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat gaatttacgc
    13621 tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa gactggattc
    13681 ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat cgactaatgg
    13741 taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa aatgttcttg
    13801 ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga atggtattac
    13861 tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat atttttgagc
    13921 gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt ctatctattg
    13981 tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag attggaaagt
    14041 ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc atgtgacatg
    14101 tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa ggttgttgca
    14161 aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg cttttattat
    14221 ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt gctcaaaaga
    14281 gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg ctaggaagaa
    14341 acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa ccaactactg
    14401 gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt cttcctcgaa
    14461 ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca gaaaatctca
    14521 aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca gcactggatg
    14581 tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt ggtatgaaac
    14641 agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt ttggacgagc
    14701 ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt cgtgatctct
    14761 gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa attgccttgt
    14821 tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa agtcttgccc
    14881 aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca gcacaggcag
    14941 caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat aattacaata
    15001 ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc attctaaaca
    15061 acattgaggt atcagaatct catttatgcg aagatactct tgaagattac ttcaagaaag
    15121 taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa actcaagcgg
    15181 ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc cgcaacaatt
    15241 ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt caattttgga
    15301 tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt ttttatagaa
    15361 agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg aaaactggct
    15421 ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc agggttagga
    15481 gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt aaaattagga
    15541 atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt ggtactcatt
    15601 gtatatttca atcgcagcta tattttttct attatacttt cttttgtata tgctattttc
    15661 aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat attaagtgta
    15721 ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc cactggcgaa
    15781 tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg tattttatgt
    15841 ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca ggagaattag
    15901 gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag atataatatt
    15961 ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt tcaggttgta
    16021 agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt tatctggaat
    16081 aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct tattaaccgt
    16141 gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt acgcaaattg
    16201 ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct tttcagcttc
    16261 ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt tacactcgtt
    16321 gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc tgttttgccg
    16381 attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg gattgcattt
    16441 gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt ttatccaatc
    16501 acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta tcagccactt
    16561 atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact tgtatttaca
    16621 cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca taaaaagtat
    16681 tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt gaaaagaaaa
    16741 aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt tgatttttca
    16801 taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt gcaataggta
    16861 tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt atattctaaa
    16921 atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa aataatatta
    16981 tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat agtaatcatt
    17041 actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa cagaaaagaa
    17101 tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat actacgcttc
    17161 aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa ctgctaacaa
    17221 tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc cttcaacaga
    17281 ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata tgtatgcaaa
    17341 atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat ttgaagctgt
    17401 tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa acttaaatga
    17461 aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact gtggtcatgt
    17521 gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa aagcgtattt
    17581 caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg ttaactatga
    17641 aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg gttcatccta
    17701 ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc ttaggaactg
    17761 tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt agtattccag
    17821 ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata tgtgaagtat
    17881 gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa ttaatatctg
    17941 aggagacaga cataaaaata acatcttata acataagttt taaagggatt tgtaataatt
    18001 gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa tgaggtgagt
    18061 ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt gtaggaatga
    18121 tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa cttgttccta
    18181 atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat ggaaataatg
    18241 ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat atagcatggg
    18301 tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat ggagaacctg
    18361 tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct tactgtaatt
    18421 tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa aaaggaggct
    18481 ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa gttttaattt
    18541 tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa tgtagtgttt
    18601 gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta agtgaagatt
    18661 gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt actgtttact
    18721 atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa ttaaatactg
    18781 aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat gaagttgaga
    18841 aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata gaacctatga
    18901 gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct caattaaatc
    18961 cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga aaaaaagcaa
    19021 agaaacctat gattatagaa aatccagttt atatttccca catgtcattt ggagctttgt
    19081 ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca gctatgtgta
    19141 gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat atttttgagt
    19201 atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat gctatagaga
    19261 taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc gaaaaagtaa
    19321 ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata agtccatctt
    19381 gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa cttagagaag
    19441 tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa aaagatatgg
    19501 aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt ggagctactg
    19561 gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt gctctttatc
    19621 gtgctagaaa atacatagat acacatggat tagatataga tttggttata acaggtgggc
    19681 ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca gttgctattg
    19741 caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt tctggtaagt
    19801 gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat atagaaaaca
    19861 gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag acttttgcta
    19921 gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat actgttaact
    19981 ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga aactattaga
    20041 ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt gtcttaaaat
    20101 gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt tatcatttta
    20161 gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt tatgaaaatt
    20221 aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa gattctgtac
    20281 tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga tacaagtttt
    20341 agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag taagctatta
    20401 tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg tattcatatc
    20461 aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt tttctcatgg
    20521 gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt tatctacagt
    20581 tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga aaagcatagg
    20641 agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta aggataagcc
    20701 atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa atgattttga
    20761 aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg attatgtttt
    20821 agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta caggatgttc
    20881 tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag atgatagagt
    20941 tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag atagtacaat
    21001 acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg tttcttttaa
    21061 ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag ggctgaccat
    21121 aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag atattttcta
    21181 tagaaaatat c
//
LOCUS       DAELTA010000060        20770 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.060, whole genome shotgun sequence.
ACCESSION   DAELTA010000060 DAELTA010000000
VERSION     DAELTA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.060"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            3..2015
                     /locus_tag="KPG13_001854"
     CDS             3..2015
                     /locus_tag="KPG13_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0985587.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            2257..4170
                     /locus_tag="KPG13_001855"
     CDS             2257..4170
                     /locus_tag="KPG13_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985588.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4163..6382
                     /locus_tag="KPG13_001856"
     CDS             4163..6382
                     /locus_tag="KPG13_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0985589.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6491..7321
                     /locus_tag="KPG13_001857"
     CDS             6491..7321
                     /locus_tag="KPG13_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG0985590.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7311..10778
                     /gene="addB"
                     /locus_tag="KPG13_001858"
     CDS             7311..10778
                     /gene="addB"
                     /locus_tag="KPG13_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG0985591.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10778..14605
                     /gene="addA"
                     /locus_tag="KPG13_001859"
     CDS             10778..14605
                     /gene="addA"
                     /locus_tag="KPG13_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG0985592.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14651..15862
                     /locus_tag="KPG13_001860"
     CDS             14651..15862
                     /locus_tag="KPG13_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG0985593.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15849..19376
                     /locus_tag="KPG13_001861"
     CDS             15849..19376
                     /locus_tag="KPG13_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0985594.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19507..20760
                     /locus_tag="KPG13_001862"
     CDS             19507..20760
                     /locus_tag="KPG13_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0985595.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         9012 a         1968 c         3691 g         6099 t
ORIGIN      
        1 atatgagaag aaatacaaaa ttattaacaa cagggattct ttcaatggca atcgtcgcac
       61 ctacaatggc atttgctact gaatctaatg ctatggaaaa taacgctgat ttaaatataa
      121 acttagagaa aaaaagtatc gttttaggta gcaaatcaaa agttagtgtc aaatttaaag
      181 aaaaaccaga tgcagatagc attacattaa agtataaatg ctatgacatg ccattgaata
      241 caactctaaa ttacaatcaa tcaactgggg catatgaagg aactatcaat tataaccaag
      301 acccagaata tctaaatgtt tgggaactac aagggataac aataaacagc aaaaataatc
      361 ctaaaacttt aaacagacaa gacctagaaa agctgggatt aaatttaaaa gactataatg
      421 taacacagga atgtataatt gaagatataa cttctagaaa agatgtaaat aaatatttga
      481 gaaaaacttc ttcacctatt acagaactta caggaagtga tagatatgaa acagcagtta
      541 aaataagtaa agagggctgg aaaaatggtt cagataaggt agttataata aatggggatg
      601 taagtataga tggcattata tcaactccac tggcaaccac atataatgca ccaatacttt
      661 tggttgaaaa aaacaatgta cctaatagtg taaaatcaga attaaagcgc ctaaacccta
      721 aagatataat tataattgga gatgagaatg ctatttctaa aactactgct aatcaaatta
      781 aatcaactgt aaatgctagt caaacacgtt taaatggttc taatagatat gagacatctt
      841 tattgatagc aaaggaaata gataaaaatc atgatgtgga aaaagtatac ataacaaatg
      901 ctaatggcgg agaagtggat gcacttacta tagcagcaaa agcaggtcaa gacaagcaac
      961 caattatatt aactgataaa gatagtatta cagacaatac atataaatgg ttaaagagtg
     1021 aggatttaca aaatgcttat tttataggtg gtcctcaaat gatatcaaca aatgttataa
     1081 ataaggtaaa tggaataact aaagatagtg ttactaataa tagagtatac ggagcagata
     1141 gacacgaaac aaatgcaaac gtaataaaaa aattctatac agatgatgag ttagaggctg
     1201 ttttagtagc taaatcagat gtacttgttg atgctttagc agcaggtcca ttggctgcga
     1261 acctaaagtc tccaatactt ataacaccaa agacgtatgt atctgcatac cataaagata
     1321 atttagaagc taaatcagct aataaggtat acaaaatagg aggaggattg acttctaagg
     1381 taatgagctc tatagcatca tcattatcta aacacaatac gactccaaca gaaccaggaa
     1441 atagtggggg caagacagtt atgattgacc cagggcatgg tggttcagca cctggaaatt
     1501 catctggagg aatgattgaa aaagattaca atttaaatac ttcacttgca acaactgaat
     1561 atttacgttc aaagggattc aatgtaataa tgacaagaga cacagataag actttatctc
     1621 ttggaaatag aactgctcta tctaattcat tgaaaccaga tttatttaca agtatacatt
     1681 ataatggctc aactaataaa caaggtcatg gtgtagaagt attttataag cttaaagata
     1741 aaaatggagg gactactaaa actgtagcta ccaatatatt aaatagaatt ttagagaaat
     1801 ttaaacttac aaatagaggt ataaaaacaa gagtacttcc tagtgattct acaaaagatt
     1861 atttatacgt tttaagaagt aatgatatgc cagctgtact tgtagaatgt gcatttttgg
     1921 ataatgaaaa tgatatgagt ttaataaact catctgcaaa agtaaaagaa atgggtacac
     1981 aaataggtaa aggaatagaa gattcattaa aataaatgga aataaaaata tttaaaaact
     2041 taatgtaaat ttatagttgt aaatttatac taaagtcaaa aaatgaaata taagtgtatc
     2101 aatagtaaga ctgtatgtac atttgcacgt gtgaatgcaa gtatatatac agtctttttt
     2161 tgtagttaaa tgcaataatt tgaatgtgtt ttgtaaaata cacaatatga tataattaga
     2221 taatatacac ttaatttaat tgaggaaggt gattatatgg aatatttatt tgaaaaaagt
     2281 agtttaaaag aatatggaaa tgaagcttta gataaatata gagaattatt agataaaact
     2341 ggtaataatt caaggtgtat tcttttgtta gtaccaaaca ataacacaaa gatacgatat
     2401 gaaaggtctc taagattaaa ttatagtgaa gaactgaaaa taacaacata tataagtttt
     2461 gtgaagaaag aattagttaa atactggcca ctaataatag aaaaatgtga tgaaataagt
     2521 acaaaggttg tatctccaac atttatatca aatagtttaa gtgaatattt aatagtgcaa
     2581 aaagttaagg aaaaaaggat acaagaagga tattttgaag atattacagg aaccaataaa
     2641 tccatagcaa atagtataat gactaacata aataagtctg catttaattt aattgatttt
     2701 gattttattg gagaaaaaat atattcatca aagaaaaata aagatagtat atatagattt
     2761 tcatacactc aaatggatga aattatatca tattatataa attccttact atcaaattca
     2821 attttagata atgcattaag catatattta tacaatcaat acctacttaa tgatgatttt
     2881 tatttaaaaa acttatttac agaagtgaga tatataattg tggatagcct tgaaagctca
     2941 agtaatgcag aagttgattt tataacagaa gcattggata atacattaca aagttatata
     3001 tattttgatt atagtaggga ttattctgta tttaataatg tggatataga ctatataaat
     3061 gagaaaataa tatcaaaaat taagcctaaa gaagaaaata ttaaaaagaa ggagattaga
     3121 atagaagatt tatacttatt acctgcaaat atagaattaa atcaatcaag tcaattatat
     3181 aatgaaatgt tagatttaat aagtgaaaaa gttatatctc ttatagaaga agatgtatct
     3241 ccaagagata tagcaattat atctcctata aacaatagta tattggagca tcaaattaga
     3301 gattctttga tagaaaaaaa tatagatgtt tttagtacta agaaggataa aaaagctgtg
     3361 gattatccat atgggaatgc attggtagtg gctacatgta tattttatgg atatttagat
     3421 tttataaaag atgatgaata cttaagcttt ttagaaactc ttttagaaat aaataggata
     3481 aaagcattta agatttttaa tgaaacaaga catttaagta ttgataatat agatgaacat
     3541 agtcagtatc gtgagatttt acagtatatt gaagaaaaaa agaagagtga tataaaaata
     3601 catgaatttt taactcaatt ttacatagat aaaatgttaa atttaaaaga aggtaaaaag
     3661 aatgttgggt tatgtaaaaa gataatagct gaaagtgagt cttttagtga aagtttaagt
     3721 ctacttggga tgaaaaaaga aaagatattt atagaagctt taaaaagtac aataaacgat
     3781 tattactctg ttgtcgatat agaagagttg aaatccaaag acaaaatagt aataactact
     3841 ccttattcat atatatcatc aaatatagat agaagtatac aaatatgggt agatatagga
     3901 agtaatgctt ggaatatgaa aatagaaaaa gatataagta atttaattgt acttagaaaa
     3961 tcttttgaag aaaaaaagat ttatacaaat gaaatggaag aatattataa aaaatactat
     4021 ctatacaata cagtatataa tttgttatta aatgcaaaga aaatctatgc atttaaaagt
     4081 gaatatgcga ttaatggtta tatccaagag agtatattat atagtatact tttaaaaatc
     4141 tcacataaag gagataaaaa ttatgattaa tataaattat agagaagacc agataccaat
     4201 aataaattac gataatggta ctatggcagt accagcagta ccaggtgctg gaaaaacatt
     4261 tataataaca aatttagtag ctaaactatt acttgagcag aaacataagg gtggtaaaat
     4321 attaattctt acatatatga atagtgctgt aaataatttt aaaggtagaa taagaaaaat
     4381 tcttgaagag aataaaatag atgatactaa ttcgtatgaa gtaatgacta tacacagttt
     4441 ggctgtaaaa ataataaagg aaaagccaga agttgtaatg ttaagtgagg attttaacat
     4501 agctgacgat ttgcaaaaaa gtattatatt aaatgaatgt ataagtagat ttagatttga
     4561 aggtggagaa agagctttta gatggttttt aaaggagcaa aaagatgaac gctggaaaga
     4621 aataactctt gaagcctggg aaagaggttt ttttgaattt gcaggaaatg caataagtga
     4681 acttaagtat aagggaattt ctccagataa ccttgaagaa atattgtcaa gaggacacaa
     4741 aggaatgctt aaaattattt taccaatata taaattgtat gatagaaaat taaagcaaaa
     4801 tggattgcta gattatgatg atatattaat tttagcagaa aaaactttaa gcttagacga
     4861 agggcttaga aagaagtttc aaactagata caaatatata tttgaagatg agtgtcagga
     4921 ctcaaatgaa attcaaggta atataataaa aattatatct agcgaaaata aaaacctagt
     4981 tagagttgga gatataaatc agagtataac aggaacattt tcatcatcag accctaaatt
     5041 ttttaaagaa tttataaata gtgcagattt ctgttatagg atggatatgt caaatcgaag
     5101 ttcaaaagat atacttgatt tagcaaacac tcttgtcaag tatgttacaa aggaatttag
     5161 acagaaagaa tgtagacatg ctcttgaaga tatgcaaata aaaactgtgc caaatggaat
     5221 gggatataaa gaaaatccta atccagaaca ttataatata aatgtaaagt ggtatgagag
     5281 ttggaaaatt gaaatagagc aaactgctag atatgttaaa ggtataaaaa ataaatatcc
     5341 agataaaagt ataggtatat tggttccatt taatgaacaa gtgacacaag ttgctaaaga
     5401 acttagagag aataatctaa cttttgaaga attaggacca aattctttga ataaaaggaa
     5461 ggttataaac aacatagcat atattattga ttttatgtta cattgtgatg atgtagaaaa
     5521 gttaatagtt gtacttaata aagtttttat agatactgat aatgaagaag aaataaagta
     5581 ttttttagaa gaattaaaaa actattctac tgaagaaatt atttataatt ttgaatttag
     5641 taagttagat aatgatttaa tagaatttaa agaagaaagt gacatatatt taagttttaa
     5701 aagaggaata gatattataa aacagatatt agaatatcca ataataaggt tagacctctt
     5761 agttttgttt attgggaaaa aacttaattt agaaaaagaa gataaagctg tactggatta
     5821 tatatctttt tatataaagt atctagtatc agaaaatata aatatggatt tagaagatgt
     5881 atataattta ttgtttgatg taaagaacag ggtgtttaat catataatag atgtggtata
     5941 tgagataaat ggatatgaac cagagcccgg tagtataaca ctatgtaact atcataaatc
     6001 aaaggggatg gaatgggatt gtgtgttttt actaggtctt gttgaatata atttccctga
     6061 caatattaat caaaagtttc agagtgataa atggtatcta aaagaaaaat ataaaaaccc
     6121 tatggcaatt attaaatcag aagttgaagc tatattaaaa ggaagtattt caacagatta
     6181 tgctcataaa acgaagattg attctataaa tgaaaagatt agacttttat atgtagggat
     6241 tactagagca aaggaaatgt tagtactttc aggaagtgct tatagagatg aaagtgatat
     6301 agggaataag agaaaagagc aaaaaccatg tatatattta agcagactta atcaacatat
     6361 tattgaaaaa aggtcaaatt aatatataat aagttattaa aagattgctt gaaatatatc
     6421 taaaagggga taaaacaata tgtacagtaa gtaactacta aaaaataaag ttaagagaga
     6481 agtgatatga gtgaacgata aattaaaata tttttcttat agtcaaaatt caattaacac
     6541 atataaatca tgtccattga aatttaaata taaatatatt gatagaataa attggaaaaa
     6601 tgatgatgtt ggtagtagag agtattatga aactttaaaa acaggaagag attttcattt
     6661 gatttgtgaa agatatttta gcaatatacc tcttggaatc tattttaatg aggatgataa
     6721 aaatagtaaa aagtttttaa agtggatgga aaatataaaa aaagttgttc ctattgaaaa
     6781 aggaaaaacg tatttgccag aatatgaagt aaggatgaca ttaaatgggg atataataca
     6841 ggcaaagtat gatttagttg taattggaaa taacaatata gaaatatggg attggaaaac
     6901 cgagtctaaa aagattgact ataaacacgt agaaaataga atacaaacta ttgtttatat
     6961 gtttttagca aaagaagtaa tacctaaagt tttaaaaatg gacataaatg ttaaagatat
     7021 aaatatgaga tattatcaac ctgaatttga tgacttacca attaccattt tatatgatga
     7081 agaaaaacat gaagcaaata gaaataaaat tcaaaattat ataagtatga taaaaaacac
     7141 taattatgaa gaacatacat acgaagacaa tctatacaat gatatagaaa aggtatataa
     7201 taaagaagaa cgtatgtgct atagaaataa aaagcattgc aagtattgtg aatttaataa
     7261 gttatgtaat ggcaaagaaa tagattatag tattttggag gcggaaattt atgggactta
     7321 ggtttgtatt aggtagaagt ggaagtggaa aatccaccta tatactagat gaaattaaaa
     7381 aagaagctca aaaaaatgaa acgacatcaa ttatactttt agttccagaa cagtacacat
     7441 ttgaggctga aaatagagta agtaaactat ttttaggaaa agaaaaagat aagtatctaa
     7501 gagttagggt acttagcttt aagacactaa gtaatatagt tttttctcaa gtaggaggat
     7561 tgacagatgt aaatataaat tctagtggaa aagcaatgat ggtatataga gctattgaag
     7621 atgtaagtga agaattaaat gtgttttcaa aatcaaaatc gcagtctggt tttgtaagtt
     7681 caattacaga tatgatatca gaaatgaaac aatacaatat aagccctgaa atgttagaaa
     7741 atatatctgg agaattggat aatgaaactt taagtttaaa attaaaagat ataagtaaaa
     7801 tatataattc ttttgaaggg aaattgcatg aaaattatgt agatgctcaa gatatgttaa
     7861 cttcattggc aagtaaaatt gaattaagca gttatttaga tggagcttgt gtttatatag
     7921 atgaatttac aggttttaca ccaaatcagt acaatgtcat aaagagtata ttaaataagt
     7981 ctaaaagtgt taatatctct ttgactgtag atgatatcaa ctatattggg tacagtaaat
     8041 ctgatatgtt ttctagaacg aaatttactt attcaaaatt gactcaattg tgcaacgaag
     8101 aaggcataaa aatattgcca caagtaaatt taaacacagg tgttataaaa agatttgaaa
     8161 aagttaagga attacagcac ttagaaagat tttataatgc ttatccatat aaaatatatt
     8221 caaatccaac tgaaaatata aagataaaag agtttaacaa tttatattca gaggtggaag
     8281 agatagccag agaaattgta catttagtta gagataagaa tgtcagatat agagatataa
     8341 ccattgctac aagagattta aatagatatg attttttagt acactctata tttaatgaat
     8401 ataatatacc aaactttata gataagaaga gagaggcaaa aagtaatcca atagttattt
     8461 taattatttc agctttagaa atgaagaata gacgatatgg gtatgaaact atgtttaggt
     8521 atttaaaaag tggacttata ggaattgata atgatgatat aaatttacta gaaaattatg
     8581 tgttggctaa tgggattaaa ggtaaaaaat ggtttgatga aaaatgggat tatagaataa
     8641 cccaaagtct ttcaggtcaa gaaagtgaat ttgaactgga attaaaagag aaaataaatg
     8701 aaataaaaaa tagagtatta gagcctatag taattttaca agaaaaatta agaggaaaaa
     8761 atagagtaaa agaaatctgt agatatatat atgaattttt attagatata aatatgccag
     8821 aaactataga aagtttaata gtaaatttta aggataaagg ggaacttgat gttgcaaatc
     8881 aatactcaca agtttgggac atagtagtgg acatattaga ccaaatggtg gaacttatgg
     8941 gtgatgaaat aatttcttta gaaaaattta taaaattaat aaccttaggt tttgatgaat
     9001 atgaattagg actggttcct cctagtatag accaggtact tgtaagtagt gtggatagaa
     9061 tgaaaaatcc agacacgaaa tatttatacc tagttggaac tactgatggt gtgtttccgc
     9121 tgattacaaa ggatagtgga atattgagtg ataatgatag ggaaagtctt ggaaacaaag
     9181 gaatcgaagt tgatattgat agtaagacta ggagttttga agaacagttt cttgtatata
     9241 aagctcttac ttcgacatct aagaatttaa ccataacata tccaatatca gaccatgaag
     9301 gtaaaacgct tagaccatca ataattatat caagacttaa gaagatattt ccaaatatag
     9361 aaaataaaag ttatttagtg gaagaaaata aaagcactga taaagatata cttaaaaaaa
     9421 taacggttaa atcccctaca tttaatgaac ttataaacgt aataaaaaat tatgacagtg
     9481 atggttacaa tacagaagag attaatagta tttggttaga tatatatagg tactatttaa
     9541 aagatgaaat ctatagttct ataacaaaaa aagttataaa aggtcttagt tatactaatc
     9601 aagttcataa gattgaagag aaaaaaataa ggtcattgta taaaagtaat tctcttagca
     9661 tatctaggct tgaaaaatat gctgaatgtc catttgctta ctttatacaa tatggtctaa
     9721 aagcaaaaaa gcgtaaagag tattcattta caccaccaga tttaggaact tttattcata
     9781 atattttaga taggttttca aaagaacttt tacaagataa tttaacttgg agagatattg
     9841 atgaaaaata tatagaatta aagataggta taatagttga tgaaataatt ttaaagatac
     9901 caggatatat attaaatagt tctgagaggt ataaatacct tgcatataga ttaaagaata
     9961 tgttgacaac tgctataact ataattagtc agcaaataaa acaaggttca tttgaaccaa
    10021 tagattatga agttaaattt ggcaacaatg gaaaatatcc acctataaaa atggtgttag
    10081 aaaatggaca agaagtaagt ttaattggac aaatagatag agttgatgaa tttgaagaag
    10141 gagaaaataa atatataaga ataatagatt ataagtcagg caataagtcc ataagtctaa
    10201 cagaaatata ttatggtcta caattacaat tacttgtgta tttagatgca atccttgaaa
    10261 gtgcaaagga tgaagatata aatataaatc cagctgcaat actatattgt agaataaata
    10321 atccaatagc aaaatttaat gaagataaag atgatgaaga aatacaagaa gcaatattaa
    10381 aagaacttag aatgaagggg cttgtagtaa aagactctca catagttaaa gagatggata
    10441 aatcattaat agatggagaa aggaaaaact ctttagtaat accagtaggt cttactaagg
    10501 atggaaatgt aggtaaaagt acttcagcaa taagctatga ggatttcaaa ctgcttagaa
    10561 aatatgtaag gcatgctata aaggatttat gtgaagaaat gttaagtgga gaaatacgta
    10621 tagctccata taaacataaa gatggaacat catgtgactt ttgtgattac tctgctatat
    10681 gtcagtttga ttcaactata aaagataaca agtataaaaa tttaaataat aagagtaatg
    10741 aagagataat aaaaatgatg aaaggagatg ttaattagtg agttctccta aatggacaaa
    10801 agagcaatta gaagttatag aaagtagaga atgcaattta ttggttgctg ctgctgctgg
    10861 ttcaggaaaa acagccgttc tagttgagcg tataattcaa atgataacaa gtagagaaaa
    10921 tccaattgat atagataagt tattagttgt tacatttact aatgctgctg cgtcagaaat
    10981 gagagagagg ataggggatg ctataggaaa agccttagat gaaaacccag aaaataaaca
    11041 tttacaaaac caacttgttt tattaaataa atctagtatt acaacaatac actcattctg
    11101 tttggatgtt ataaaatcaa actttcatag aataaactta gaccctaatt tcagaatagg
    11161 agaccaaact gaatgtgcta ttttgaaaca agaagcaata gaggaagtat ttgaagattt
    11221 atatgaagaa agagatgagg ggtttttaaa tttagttgaa agttatgcag aaagaggagg
    11281 agataaagaa gtacaagata ttatcttggg aatatattca tttgcaatgg cttctcctga
    11341 acctaaaaaa tggttgattg actcagcaga acgatttaat attgatgaaa attttgattt
    11401 ttcacagtct atttgggcta gagctatatt agacactgta aaaatagaga ttaatggtct
    11461 ttgtctaaat atggaaaggg cattaaaaga agtggaatca atagaagaat tagaaacttt
    11521 tgcagaaaag ttatccgttg aatataaaaa aattgctgac atttcacaag catgtaataa
    11581 atcttgggat gaggcatata aaaagatggc aagtatgtcc tttgaaaatt atgtaaaagg
    11641 agtaaaaaga atttcaaaag atgctccttc gtatataaaa gaatcaaaag aaaaggcaaa
    11701 aactataaga gataagacta agaagtcttt agaaagtata gtaagtgcta cattcaataa
    11761 agataatgat tctattagag aagaaataaa atatctttat aacatagtta aaccaatatc
    11821 tagtgttgta ctgagatttg aagaggaata tagcaataag aaaagagaaa aaggaataat
    11881 agattttaac gatatagagc attttgcatt aaatatactt acagatgtag atgaaaaagg
    11941 taatattgta ccttctgata tagcagttgg atgtagaaac aagttttatg aaatatttat
    12001 agatgaatat caggatagta atctagtgca ggaagtactg cttaaagctg ttgcaaatac
    12061 agaaactcca aatagattta tggttggtga tgtaaaacag agtatatata gatttagaca
    12121 agcaaagcca gaattatttt tgcaaaaata taataactat aatgataaaa agggaagctc
    12181 ccatagaaaa ataatgcttt acaaaaactt tagaagtaga gaagaagttg tagatgctgt
    12241 aaattatata tttgaaaata taatgaatga gaatatagga gaaattgaat atacagaaaa
    12301 agaaagacta aatttaggtg caaattttaa tgtggataca gacgagaaat ctattattgg
    12361 tggagcaacg gagatacacc taatacaaaa agataataaa cttgatgatg atattataaa
    12421 tgataaagac gatagaatta ataacaaaga aaatgaaata gaggaagaag aaaaattaga
    12481 taatatacaa cttgaggcta gaatggttgg aaacattatc aaagatttaa tgaaagtcaa
    12541 tgaagatgga aaaattcaaa aggtctatga taagggaata gatggatata gacctgtgga
    12601 atttagagat atagttattc ttttaagagc tacatctgca tgggctccag tgtttgcaga
    12661 tgaattgatg aatatggata taccaacgta tgctgatgtt ggagtggggt attttgatac
    12721 gattgaaata aaaacaatat tgtcattact tcaaataata gataatccaa tgcaagatat
    12781 acctcttata tctgtattaa aatcacctat atttggattt acaccagagg atttgattga
    12841 tattagagtt cagagtaaag ataaaatttt ttatgaggtt ttaaaaagta cagcagagta
    12901 tgatggattt acagattctc aaaatgaaaa tgagtctgag tttataccaa gtgaagagtg
    12961 cataaacaaa agtaaagatt ttttaattaa gctaaaggaa tttaaagaaa agtcaatgta
    13021 catgagtaca gatgaattta tatggtactt gtacacaagg actggatact atgcctatgt
    13081 tggagcattg ccaggtggaa gtcaaagaca agctaattta aaagtattgt ttgagagagc
    13141 aaaacaattt gaagaaacca gtcttaaagg aatattcaac tttgtaaatt ttatagagaa
    13201 attaaaaaaa tctagttctg atatgggaag tgcaaaaaca cttggtgaga atgcaaatgt
    13261 tgttagaata atgagtattc ataaaagtaa aggtctagaa tttccagtag tcatatgctc
    13321 tgcaatgggt aaaaatttca atactcaaga ttttaaaaag agtattttat atcatcataa
    13381 tttagggtat ggacctcaat ttgtagacta tgagagaagg atttcttttc caagtatagc
    13441 caaagaagca ttaaaaagca aaataaatat agaaaattta tcagaagaga tgagagtttt
    13501 atatgttgca tttacaagag ctaaagaaaa attgattata actggttcta ctagaaatat
    13561 acaagatagt ataaagagat ggtcaaatgg aattgaaagt ttagatacaa tatcacaata
    13621 cgagatatta aaaggtaaaa attttttaga ttggataatg ccttgtgtat taagacatag
    13681 ggatttatct aatctactag aagaagtagg attagacgca gtttttaatg ttgaacataa
    13741 ttcaaaatgg tatggaaagt tgtggaataa gaatgatatt ttagtggaaa agaagagtga
    13801 tgaagaaaaa gaaagtattg aagaaattct tgaaaaaata gatgtaaata atcctgacag
    13861 tgattattac ggtgaaatag aagaaaaatt aaactatata tatccatatg aatttagtac
    13921 aagaaaacct gctactatat ctgttacaga aataaaaaag atacaaaata attatgaaga
    13981 agagttaata aatactatat ttgaacaaaa agtaatatta aaaaagcctc tatttattca
    14041 aaatgaagaa gaaagagaaa aaataagtgg aacagaaaga ggtacaatag tccatttagt
    14101 tatggaggtt ttagacttaa aaaatgtaag tagtgtaaat gatataaaat cacaaataag
    14161 aggttttgtt tcaaaaggta taataacaga aaaacaagct agtatagtaa atccatataa
    14221 aatatataaa ttttttgcct ctaacatagg aaaaagaatg ttgaatgctg aaattataaa
    14281 tagagaaaaa tcaatttatg cacaagtaaa tatgaaagac atatatattt atgaaaagct
    14341 tataaacaat gacgataaaa aactttatga taatgaaagt gtcatgttaa gaggtatagt
    14401 tgatgcttat tttgaagaag ataatcaaat agttttggtg gattataaaa cagattttgt
    14461 aaatgaggaa aatataaatc aaataataga gaaatataaa aaacagttgg atttatatgc
    14521 agatattatt gaaactttaa ctggtaagtc agtaaaagag aaatgtatat atttatttgg
    14581 agttgatgaa gctgtttgtt attagttttt atatataaat taaacctaaa agatagagga
    14641 aggggaaaaa atgagattta ttcatacatc agattggcat ctagggaagt ccctagaagg
    14701 ccattctaga attgaggaac aagcaaagtt ttgtgaggaa tttataaaaa tagtagaatc
    14761 aaatgaaata gatatggtta taattgctgg agatgtatat gatacttcta atcccccagc
    14821 acaagcagaa aaactatttt atcagactgt atcaagactt gccaataatg gtcagaggtg
    14881 tgtattaata atttcaggaa atcatgataa tccagagaga ctctctgcaa taacacctct
    14941 agcacatgaa caggggatac ttatatatgg ttatccatta agtgctacga tagaagctaa
    15001 gtataaaggg tttgaaataa catatgcaac tcagggatgt acaaaactga atataaatgg
    15061 agaaaatatt gtggttgcta cattacctta tccaagtgaa aaaagattaa atgaagtttt
    15121 ttcaagtgaa gatgagtttg agaaacaaaa aaattattct gaaaaggtag gagatatatt
    15181 tagaagttta gaagaaaatt ttagaagtga tactataaat atagccgtat cacatatatt
    15241 tgtaataggt ggagagagta ctgaatcaga aagacctata cagttaggtg gaagtttttt
    15301 agttgaaaga aaagatttgc cagaaaaggc tcaatatact gcactaggtc atttacataa
    15361 acaacaaaaa gcttccgaac gtttaaatgc atactattct ggttctccac ttcaatatag
    15421 caaggatgag agagcgtata ccaaaggggc atacatagtg gatataaaag ctggtgaaaa
    15481 accaataatt gaagatgtat attttaataa ttacaaacct atagaagtat ttaaatgtaa
    15541 tggaatagaa gaagcactag atatatgtga gcaaaatcaa gatagagaga tttggtcata
    15601 ttttgaaata aatactgatg agataatatc acagaatgaa attaaaaaaa tgaaggaact
    15661 attaaaagac ataatagaaa taaaaccaat aataacatct tgttatgaac aagaaagtgt
    15721 agatataaaa gaaaagtcta tggcagagtt gtttagagag ttctattctt ttagtaaagg
    15781 tgtagagcca aaaggagaac ttatggattt atttctagat ataataagtg aagaaggtga
    15841 atcagcagat gagaccaatt agattagaac ttacagggct taatagctat attgataaac
    15901 aagttataga ctttgaaaaa ttaattgaaa gaggtttgtt tgggatattt ggaactactg
    15961 gaagtggaaa atctacaatc ttagatgcaa taactattgc tatgtatgga aatatatcca
    16021 gaaatacaaa agaatatata aacagtgtgt gcgataaggc aattatatca tacgagtttg
    16081 agataggaag caaaaacact agaagaagat acattgtaga tagaaccatt gctagaagca
    16141 aaacaggaac aaagacctct aatgctagat taatagaagt attgaatgac aatacccaaa
    16201 atgttttagc tgataaagtt gtggaagtaa atgaaaaagt agcacaggtt gtaggattaa
    16261 cagcaaatga ttttactagg tcagtagttt taccacaaga caaatttaat gagtttctaa
    16321 gattatcacg tgcagataga agagacatgt tagagagaat ttttaatctt gagaaatatg
    16381 gaagaagtct tggagaaaaa gtgaaaaaga gaaaaaatat acagttacaa aatttaaaag
    16441 atttgaaatc aaaattaagt caatatgatg gaattacaga ggaagtatac aacaatataa
    16501 accaagaatt aatagagtta aaaaatttgg aaaaagataa ggataatgct ttagacctag
    16561 ctcaaaaatc ttatgaagaa agtaaaacag tatatgaaga acaattaaag ttagaaaaaa
    16621 atgaacttag aaaaaatgag cttgatttaa aaaatagtga gataaaagaa aagattgctc
    16681 ttgtagaaaa tgatagtaac gctaaaaagg tagacccata cataagttca gttcaaaatc
    16741 tagaaaaaaa gatagatgaa gatagcttcg cagtaagtaa tttagaaaag aaattagcaa
    16801 tattaaatca ggagcttgaa gttacaaaaa atagatatga aaaaataaac aaaattaaga
    16861 atgaagaagt ccctaaattg agtgaagaaa aaataagatt gcaacaagca ataaaattag
    16921 aagaagagtt agttttatta gatagagagt taaaagagct aaaagaaagt ggaattaatt
    16981 taaataagac taaagttgaa ttagaaaaag taaagcaggt gtcagagtcc agaaaagatg
    17041 cagttactaa atctataaaa gaagttgaag gaaagattga taaagtaaat ataagtgcag
    17101 aattaaaaca aaaaatattt ctagcatatg aatacgagaa agattacaac aacgtattgg
    17161 aagaaaaaaa tcaaaagcta aataaactag aagaaatact caaagagaca gaaaatataa
    17221 atttaaaagt tagatatatt gataaagata agaatgatgt aaatcgtaat ttagaaaatt
    17281 tatcacttca cttggatgtt cttcttaaaa aatgtcctgg taagagtgct gatttacttt
    17341 taaaatctga atatgttact gaattaagaa ataaagcaaa taatactaaa gaaaatgaaa
    17401 ttaagaaaag cagtatacaa gatgaattaa agataatact tgaaagtaag tttaatactg
    17461 aaagggaagt aaatttacta aatgaaaaac ttgaaaataa tagaaagaac agagatgact
    17521 tagaaaaaga attagaagaa ctaaagtatt taaatttagc ctctgaactt agacgtgaat
    17581 taaaagaaaa tatgccttgt ccagtttgtg ggtcaaaaca tcatgaaaat catgatataa
    17641 ctaagtatga tgaaaatata tcttttgtca aagaaaaact ggagaaatta gaaaaagaaa
    17701 aaataagtat aagaaacaat atagaggaat taaatgctaa agtaagtggg tatttatcta
    17761 tagaaaaaat gaagactaaa gaacttgaag atgtaaaagg aaaactaggg gggattccat
    17821 ctagtcaatt attaaaaaaa cttgatgaag aacaaagaaa acttgcattg ttaaaatcta
    17881 atattcagga gtgggagaaa gaaaaagaga gtactgaaaa taaagtaaca ttagctaaag
    17941 aagaaaaaaa taaaattgaa aaagaagaat taaaaataag agagagtctg aataattata
    18001 agaaattaac aagagattta aacatagaaa tagaaggttt agaaaataaa tgtaaaaaac
    18061 ttaaacagga atacctagga ttaaagacga ttactaaagt ctctaatcta ttgtcaaaag
    18121 taaaagaaat aagagagaat gaaaaagaat tagaattact aagtactggg cattcaaatt
    18181 tattaaaaaa tagagactca ctagatatta aaataagaga aaatgagagt aaactacatg
    18241 aaatagagat agaattaata aaggctagag aattatatgt agaaaagaaa ttgagtagag
    18301 ataataagta taaagaagtt attagcataa cgaaaggtga tttagctaaa aatcttttac
    18361 ataatgttga agaaaatata tgtaagatat tagaacaaga agaatcaagt aagaaaaaat
    18421 tggaagagca aagattagaa tatgaaaaaa acattgctga aaagcacaat atagatggta
    18481 gacttaaaac tgctaaggaa caatataaag accaaaaatc aacactaaat aaactattag
    18541 cagaaaataa atttgaaagt atatatgcag taaaaagagc tttattagat actgatgcag
    18601 taaaaaaatt gattgaagaa atactagagc atgaagaaga acaaaagtta ttatcattta
    18661 aaataaaatc atcaaaagaa aaattaaatg gaagaagcat aaagaaggag tactttgaac
    18721 agttaaaaga tgaaatttat aatttgaaag tagaaatagg aaatatatcc aaagatattg
    18781 gagctaatca aaatcagtta ataacattaa aagactcttt agataagata aatgatttta
    18841 ataaacaact taaggtagtt gaacacaatg tggacttatt ggaagaatta gataaatgtg
    18901 tacaaggaaa taaatttgta gaatatgttt ctacaaacca attaaaatat atagcgttgg
    18961 aagcatcaaa acgattggat ggaataacaa aaggaagata tgcacttgaa attgactcaa
    19021 cactaaactt tgtaatgaga gataacttta atggaggaga aagaagaagc attgatactc
    19081 tatctggtgg agagacattc ttgacatctc tatcattagc acttgctctt tcatctcaaa
    19141 tacaattaaa gggaagtgca ccattagaat ttttcttttt agatgaaggt tttggttcat
    19201 tagatagtga acttttagaa attgttatag aatcattgga aagattgcat agtaataatc
    19261 taagtgtggg tataataagt catgtcgagg aacttaaaaa tagagttcct gttaagctat
    19321 tagtttcatc tagtgaggct ggaataggtt caaaagtaaa aattgaatat agttagaaaa
    19381 tatatgttaa ttgaatatat tatctaatat ttccaaataa tatgtaattg tgatataata
    19441 aaaactaata ttaaagttat ttataattaa tattaatata aaaacatgta agtaggagtg
    19501 tggtaagtgt tggaatcgcc caataatttg attcagatta tttttttaat agtattactt
    19561 atagggtctg catttttctc agcatctgaa acggctttaa tgtcgttgag taaaatcaga
    19621 atcagatata tgcaagatga aggagttaaa ggagctaagt tagtaagttc attaatagaa
    19681 aatccaaata agcttttaag ttctatattg gttggaaata acgtcgtaaa tatagcagca
    19741 acatccatat caacatcttt gtttatagga ttaatgggag aaaaaggagt ggcacttgct
    19801 acagctgtta tgacagtatt agtattaata tttggtgaga ttactccaaa aacaatagca
    19861 gcaaataatt cagaaaaggt ttctctatta gtatctaaac ctataaaagc aattatattt
    19921 atattaagac caatagtatg gatatttaat ataattacaa atattatatt taaattattt
    19981 ggtataacaa ataaaggagc taaatcattc ataactgaag aagagctaaa aactatggta
    20041 aatgtaagtc atgaagaagg cgtccttgaa atggaagaga gagaaatcat aaataatgtt
    20101 tttgaatttg gagatatgca agctaaaaat gcaatggtac aaagaataga tatggtagct
    20161 atagatatgg aagatagtta tgatgagata atacaggtat ttaaaactga aaaattaagc
    20221 agaatgcctg tgtatgaaga aactatagat gatattgtag gaatacttaa tataaaagat
    20281 attatatttt tatctgatga agaaatagag tcatttgata ttaaaaatta tatgagagaa
    20341 ccttttttca cgtatgagtt caaaaagata acacaacttc ttgaagagat gaaacttgaa
    20401 aaaagtcaga tggctatagt tgtagatgag tatggtggaa catctggact tttaactata
    20461 gaagatttag tagaagtcat tgttggagat atagaggatg aatatgatga agaagaggat
    20521 gaaatacaag ttataaaaga agatgagtat attgtagatg gaagtactaa aattggtgat
    20581 gttaatgaac ttattggtgt aaacttggaa tctgaggagt ttgattccat aggaggtttt
    20641 ataattggac acctaagcag attgccagaa gaaaatgaag taattgaggt tgataatata
    20701 agattttgta tagaaagtat agaaaaaaat agaattaaaa aaataagaat atatacataa
    20761 tgtaataatg
//
LOCUS       DAELTA010000061        20762 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.061, whole genome shotgun sequence.
ACCESSION   DAELTA010000061 DAELTA010000000
VERSION     DAELTA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.061"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="KPG13_001863"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="KPG13_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG0985596.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="KPG13_001864"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="KPG13_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG0985597.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="KPG13_001865"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="KPG13_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG0985598.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="KPG13_001866"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="KPG13_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG0985599.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="KPG13_001867"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="KPG13_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG0985600.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="KPG13_001868"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="KPG13_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG0985601.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="KPG13_001869"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="KPG13_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG0985602.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="KPG13_001870"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="KPG13_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG0985603.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="KPG13_001871"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="KPG13_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG0985604.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="KPG13_001872"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="KPG13_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG0985605.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="KPG13_001873"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="KPG13_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG0985606.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="KPG13_001874"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="KPG13_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG0985607.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="KPG13_001875"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="KPG13_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG0985608.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="KPG13_001876"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="KPG13_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG0985609.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="KPG13_001877"
     CDS             6989..7174
                     /locus_tag="KPG13_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG0985610.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="KPG13_001878"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="KPG13_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG0985611.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="KPG13_001879"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="KPG13_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG0985612.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="KPG13_001880"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="KPG13_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG0985613.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="KPG13_001881"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="KPG13_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG0985614.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="KPG13_001882"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="KPG13_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG0985615.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="KPG13_001883"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="KPG13_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG0985616.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="KPG13_001884"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="KPG13_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG0985617.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="KPG13_001885"
     CDS             11117..11767
                     /locus_tag="KPG13_001885"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG0985618.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="KPG13_001886"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="KPG13_001886"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG0985619.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="KPG13_001887"
     CDS             12536..12805
                     /locus_tag="KPG13_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG0985620.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="KPG13_001888"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="KPG13_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG0985621.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="KPG13_001889"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="KPG13_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG0985622.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="KPG13_001890"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="KPG13_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG0985623.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="KPG13_001891"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="KPG13_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG0985624.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="KPG13_001892"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="KPG13_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG0985625.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="KPG13_001893"
     CDS             14901..15848
                     /locus_tag="KPG13_001893"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG0985626.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="KPG13_001894"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="KPG13_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG0985627.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="KPG13_001895"
     CDS             16330..17163
                     /locus_tag="KPG13_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0985628.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="KPG13_001896"
     CDS             17151..18017
                     /locus_tag="KPG13_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG0985629.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="KPG13_001897"
     CDS             18011..18814
                     /locus_tag="KPG13_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG0985630.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="KPG13_001898"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="KPG13_001898"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0985631.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="KPG13_001899"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="KPG13_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG0985632.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="KPG13_001900"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="KPG13_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG0985633.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8293 a         2449 c         4322 g         5698 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 ta
//
LOCUS       DAELTA010000062        20677 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.062, whole genome shotgun sequence.
ACCESSION   DAELTA010000062 DAELTA010000000
VERSION     DAELTA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.062"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            696..2930
                     /locus_tag="KPG13_001901"
     CDS             696..2930
                     /locus_tag="KPG13_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0985634.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2961..3806
                     /locus_tag="KPG13_001902"
     CDS             2961..3806
                     /locus_tag="KPG13_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0985635.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            4046..5779
                     /locus_tag="KPG13_001903"
     CDS             4046..5779
                     /locus_tag="KPG13_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0985636.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   6104..6525
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6237..6265
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7055..7159)
                     /locus_tag="KPG13_001904"
     CDS             complement(7055..7159)
                     /locus_tag="KPG13_001904"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG0985637.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7784..8029
                     /locus_tag="KPG13_001905"
     CDS             7784..8029
                     /locus_tag="KPG13_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985638.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8782..8928)
                     /locus_tag="KPG13_001906"
     CDS             complement(8782..8928)
                     /locus_tag="KPG13_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985639.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8961..9347
                     /locus_tag="KPG13_001907"
     CDS             8961..9347
                     /locus_tag="KPG13_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985640.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            10214..11569
                     /locus_tag="KPG13_001908"
     CDS             10214..11569
                     /locus_tag="KPG13_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0985641.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11687..12355)
                     /locus_tag="KPG13_001909"
     CDS             complement(11687..12355)
                     /locus_tag="KPG13_001909"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG0985642.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12422..12850)
                     /locus_tag="KPG13_001910"
     CDS             complement(12422..12850)
                     /locus_tag="KPG13_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0985643.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12957..13550
                     /locus_tag="KPG13_001911"
     CDS             12957..13550
                     /locus_tag="KPG13_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG0985644.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13669..14604
                     /locus_tag="KPG13_001912"
     CDS             13669..14604
                     /locus_tag="KPG13_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG0985645.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14875..15819)
                     /locus_tag="KPG13_001913"
     CDS             complement(14875..15819)
                     /locus_tag="KPG13_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0985646.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            16137..17567
                     /locus_tag="KPG13_001914"
     CDS             16137..17567
                     /locus_tag="KPG13_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985647.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17767..18351
                     /locus_tag="KPG13_001915"
     CDS             17767..18351
                     /locus_tag="KPG13_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG0985648.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18455..19234)
                     /locus_tag="KPG13_001916"
     CDS             complement(18455..19234)
                     /locus_tag="KPG13_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG0985649.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19292..20548)
                     /locus_tag="KPG13_001917"
     CDS             complement(19292..20548)
                     /locus_tag="KPG13_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG0985650.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
BASE COUNT         7812 a         2800 c         2533 g         7532 t
ORIGIN      
        1 aatctttaaa acaaggatag gaaaataaac atcctaataa ttaattatta aaatattttt
       61 atattgtaat attaggtata gtatttataa cttcaacttt tttataaata tattttagtt
      121 ttatatagtt ttatgtttac agctattata tccatctcta tgtaatattt ttcatattaa
      181 aactgatttc tatagattaa tagtttataa atttagtaaa tgaatattta ttatagtctt
      241 taacaaaaaa tacaagaata aacttaattt taataactaa aattaattat gcaaaattat
      301 taatatggta ttaaattatg tataaaaagt taattatgaa taaaataatt atgcctttga
      361 aaaatcatta aacttaaaat taattataaa aaaactctac cttactaact atacagctca
      421 caatctatct atttatgaaa aaatatgata taattgcatt caaaaaggaa atctttcaat
      481 atactagaat ttaagcatta actaatttta tctaatatta ttactaaact aaaaaatatt
      541 tgtatttgta gcattggagc aaataactta atgtatattc attttaaata cttatttaaa
      601 atttagcatt taaattattt acaaattaac atttaaatac ttctagcata aatacagttg
      661 tcatcaaaat ttaaagtatc ttatggagga ataccatgca aaatcataat tatataaaaa
      721 tgttaaataa aaaggtagaa gtattatcta aggaattaga tagtagaaat tttaaattaa
      781 aaaccttaaa tgaaatagat tttttttcta aaataccaaa cagagaattt atgtaccaac
      841 atattgatga ctgtatttcg aaaaatatac atataggaat catgttatta aatatcgata
      901 actttaaatt atttaatgat atatatggat acctagtcgg agaaaattta ttaattactc
      961 taactcaaaa cctattgcac tttattccag aatcaaaggg aatagtcgct tatttaaaag
     1021 gagaaggttt tttaattgta cttccaaatg tatcaaataa agagttaaat aatatgggtg
     1081 caactattat agagagcgcc cgagaattaa aaataagact tcctaatgaa aaagaaatat
     1141 actccaatgt tacatttagt atcggcagca ctatctggaa tggtgaacca aacatgacta
     1201 ctttgatgct tttcaaccaa gtgtatagtg ctttaaaaaa ggctaaaagt gaaggtaaaa
     1261 atagatacat tctatttgat tttaaagata atagttttta caataaatta gaaaatgata
     1321 aggagttctt atcagaaata tcacatgttc tacaaactaa acaaattgaa gtattttacc
     1381 aaccacttta taatataaaa aataatacta tagttggatg tgaggcactt gcaagatgga
     1441 gacaccctat taaaggactt cttactccag ataaatttat tcctttattt gaacagtttg
     1501 gtctgataac ggaattagat ttgcatttat ttgaagagaa ctgtaaaaat ataagaagat
     1561 ggattgataa caatgatgtt tttgttccaa tttgttgtaa cttttctcgt cttcattttt
     1621 ttaaccctaa ttttccaagt atgctaaaag gtattactga aaagtacagc gtctctacat
     1681 caaattttag cattgaaata acagaaaata tattaataga aaatacaaat acaattattc
     1741 aacaactagc tgaacttcgt tcttatggtt tttctgtatc aatggacgat tttggttctg
     1801 gatattcatc ttttggtatg ttgcaaaatc taccttttga tataatcaaa attgatagga
     1861 tatttttatc tcataattta aatgatttta aaaatacaac cattttgtac tctataataa
     1921 acattgcaag agctcttggg atgcttacag tatgtgaagg tgttgaaaca gaacaacatg
     1981 tagaatttat taaaagtata aattgtgata tcgcccaagg ttattattat gcaaaaccca
     2041 tggagtgcaa agaatttgaa aaattactat ctaaagagaa taattcagag aaaatgttta
     2101 taaaatattc tgatatagga aaatctttaa tagagaagat atttgatgga ttttttatta
     2161 tgcaagatat gaaaaaacta agagctaatg tcactgaaga cattcaatgg tttgattttt
     2221 acaacaaaac tgaaattaca tcttttccta aagtaataga gcattttgaa aaacatatta
     2281 aaggtaaaaa attttctatt ctttacaaaa gtataacccc acataatact gaatcagatt
     2341 cattattaat tagtggagaa gccgttttat cagatgaagc ttctgaacat caaaaatata
     2401 gcaatttcta ctttagtgca aattgtataa tttcagaaaa taaattactc ttagcaaaat
     2461 tacatttgga tttaattaaa actgctggct atataaatga atttgaatca tattcaatac
     2521 ctaaaatgac aaatacacat cttgatgaaa gtaatatttt atcacaattt tattcaatgt
     2581 tgcctgtagg tataattcgc tacgatttat ttggtgatat gattattact tatatgaatg
     2641 aagaaatgtt tgatattata ggttatacaa aagaacagtt ttttggtgag atgggcggaa
     2701 atttaagagc aattgttcat ccagatgatt tagactttgt ttataaaaaa agtttggaca
     2761 tgattgacac aaataaaatt gaaccatttt tatatagatt cataaaacga gatggtcaaa
     2821 ttgtcacagt tatgtataca caatgtaacc tttctgcaat tgatggaagg tcgattgccc
     2881 agggtatgta tgtagatgta aataaaataa aaaatttaac aaaaatctag tacaaagtat
     2941 tttattttgg agaaattaat atgtttaaag aaattttttt aagaactttt ccaggatttc
     3001 taattataag agattctaac tacagaatta tatttataaa tgataattta aaaaatttaa
     3061 ttaaaagtta tatgcaagat aatccacttg gaatgactaa tatagaaata gctaagaaac
     3121 tccctgataa tattgctaaa ttttttacgg attctcacaa tattcaatta gattgggaaa
     3181 aaaattatcc ttatgataaa atttcaaatt ggattttaga gtttaaaaaa gatactacaa
     3241 gctattggaa tgttttagaa tacaaagtag atgttgatga aaaaacctac ataattacta
     3301 tggccaatga tataacaaaa ctctatgaag aaaataagag aaatcttcac tactcaatta
     3361 ctgacccatt aactggagca tataatagaa aatatttaaa tgatagattt gacatattta
     3421 taggagatta tattgtacta attgacttag ataatttcaa aatgataaat gactatgaag
     3481 gtcataatgt aggagacaag attttatgtg attttgtttc acttttaaat aaagaattaa
     3541 taaattctac ttctattata cgattaggag gagatgaatt tattgttata ttctctagtg
     3601 atgttgacaa aaattatgtt tattcacagc tagaagcctt aagagaaaat tttttaaaag
     3661 tattctctaa gtataagtat ctttcattta gttatggtgt tgatacagta aaaagaaatt
     3721 taaaattgac aatagttgaa ttggataaaa aaatgtataa aaacaaggag aaaaacaaga
     3781 aaaaatttga taaaaatgat tattaacatt gttattttaa aataaattaa aattatcttt
     3841 ctttttttaa acttgtacat tagtttataa atatgtatct atacaagata ttgacattaa
     3901 gtactgaagc atatattgtt tcagttcttt tttgttaaaa ttaacttatt taacaaagat
     3961 atcatagttt aacaaagctt ttaatttaaa tatattgatg tataatataa ttaaaatata
     4021 atagcaaaaa gaaaggagga atatcatgag catactccta aaaaaagcac ccaaattagc
     4081 aaaacatatt ataacaagtt tttatataaa tcgagatatc aatgaagtat taaaatacct
     4141 ttgtgaaaat gttacatgga ttggaccagg agaacaggaa tttctcactt cttttaatga
     4201 aataaaaaat tatttttatg caggtcaaga tgaaattcct tcttgtgata taaataatga
     4261 tatatttgaa actgtaagtg aagatgagaa tagatgtatg gtattaggaa gatatactgt
     4321 tcgaactaaa gaaaatgctc aaatgatttt agaggtaaat caacgttgta catttgagat
     4381 aattgaggat agaggaaaat tattaataaa gcacatgcac atatcaaacc cttatggtga
     4441 aatgcaactt gatgaatact ttcccactaa aattggaact caaagttatg attatttgca
     4501 aagattacta aaagaaaaaa cagaagttat tgaaatgatt acaaataata tcaatggtgg
     4561 cttaaaaggt agcaatgatg atagtacata ctcatttttc tatgtcaatg agggattgcc
     4621 caaaatatta ggatacacat atgatgagtt catggaaatg agtggtggtt cagcagtggg
     4681 tgctgtatat ccaccagact tacctaaagc actagaagat tgtgaacgat gttttgcaaa
     4741 aggacctaca tattcatctg aatatagaat ccgaaaaaaa gatggaacat taatgtgggt
     4801 attagattct ggaatgaaat cgttaaatag tgatggcata gtaaaaatta atagtattat
     4861 tactgacata acccaactaa aaaacataga atctgaacta aaattagaaa gagagcgata
     4921 tcgtattgct ttgcaaaata ttacagatat catgtttgag tatgatattg aaaacgatac
     4981 ccttataaaa tatcaacgtg tagaaattga caaaaaaatt gaattagaaa attttgaaac
     5041 aaaaaattac tccaaactat tagaatctgg taaaattatt caccttgatg atattggaaa
     5101 actactagaa gtacttcatg gaaatcttcg tgaaacaatt gagataagac aagtaaattc
     5161 attaactaaa aatgaatgga gatggattcg tgttcaatgt tcagttatat atgatagtga
     5221 ccataaccct atcaaaacaa taggcgtttt aaaagacata actgaagata aatctaaatt
     5281 agaaagttta attaatcagg ctcaacgtga cccactaaca caattatata atcaaagagt
     5341 atcacaaaat cttattgaag agtatctatg tagttctgat tccaaaaata atgatgctct
     5401 attagttatt gatattgatg attttaaaac tgtaaatgat acttttgggc atctagaggg
     5461 aaatgaagtt ttagttgctg tatcaaaaat actattacat aacacacatg ataaagacat
     5521 agttgctcga attggtggag atgaatttac aatatttata aaatcattaa caaaggattt
     5581 aataataaaa attactaatg atattttaaa tgatgcgagt aaaataaaag taaaggacaa
     5641 tcataaaata acattaagta ttggtattgc cttcactgat gattcaacta aattatataa
     5701 agatttattt tctaaagctg ataaagctct ttatctatca aaagcagatg gcaaaaattg
     5761 ttatagtaca tacgaataat aaagctattt atacgaccaa gaatgaacct ttaaatttaa
     5821 tttcatagtc taacaatcaa agagttgaag ctaatcttat agctagtcaa ctagttccta
     5881 gaaaaaattt aaacaatata tttattgata ctgctcattt ttctaaagag caaatttata
     5941 taaaaaatta aacttttata tacatacaga tactaatgat aaacagttat cattagtatc
     6001 ttctatctta aaacaaaatg ataatttatc tatataaaaa agtgctttgt tgacagagtt
     6061 cactttttta tataggtaca gaatttaagt ataaatacat gttttattct atacatttta
     6121 gttaatgtaa aatttcttga ttgtactctc taacttttca acattatact atttatatac
     6181 cacttagtta atataaaact ataaaagtgt tgcaattact gggttttata aagttaattt
     6241 acataccact tagttaatat aaaactttag caccttattt atctatagag ctacagaaag
     6301 tatttacata ccacttagtt aatataaaac atgtatttat ttgttcttgt actattgttt
     6361 tactctattt acataccact tagttaatat aaaacaatat caaaacaaat aaaaattttt
     6421 aataaatcat tatttacata ccacttagtt aatataaaac tttggagttg ttacagatta
     6481 tttgccttat ctctctattt acataccact tagttaatat aaaaccccca aataaactgg
     6541 gcattttcaa tacttacaca tatgcaactc tcttaaattt gcagtgaacc atgagtagtg
     6601 caaatgataa agcttatcat ataccctcaa caccttatat ttcaagcgtt aagctctact
     6661 ttgtaacaaa tatcgctcac tgcaaaacta tttacatttt tattatatca taaatatatt
     6721 atttttgaat atctatacca atttgtggta taataaaagc aagaagaact acaatctatt
     6781 tagacgtaga gtgaagttca aatactaatg tttattcttt ttgaacttga tttttagttc
     6841 aaaactaaag tcactcctgc ctgtgtgaac tatacccgct gttttataca gttaaaacat
     6901 gtaaaaatag ttataaagta ctagtgtaac tcattagtac tctttttatt atattcatat
     6961 aattaatatc tatattttaa agcttactat ctaaaaaaga gcacgtcaaa taaaacccta
     7021 gatatttaat ttttttagag atacttaaaa taaattatgc tctctctgta cttaaatatt
     7081 tcctaaattc aaatggagac atagaattta atctattttg atatctaaaa ttattatagt
     7141 agtatatata atcatctatt gctttaacta attattcata tgtatcaaat ttccttaaat
     7201 agtacatttc tacctttaaa attccccaaa atccttccat aggagcattg tcaaggcaac
     7261 gaccaactct tgtataatat atgtatatct aacaccatta agaaatatat attttataat
     7321 atatatttct tcatttaatg cacttagaaa agcaaagaaa atacagatta gtacactttt
     7381 tagagaaaaa taaaattaat acatctaaaa tattaaaaaa tactttagat aatctaaaga
     7441 cacatctcaa agtttttaac taaattataa aactaattac aaatgctatg taacgataat
     7501 aaataaaaaa tttacttcgc ttaagcgacg tgtcgttgaa atatttcatg ttgccaactg
     7561 attcataagt cgataagaaa agaacactaa ttatttagag ttcttttctt agctacaagt
     7621 ggattataat acttttaaat ctgtgagaaa atcatatata ataatgatat gaacaagtaa
     7681 agaaaataca aaaatgacaa aattataaat aaaatattat aaaatcataa ggtcataatt
     7741 gttatattgt agaaacaaag taaagagaga gggtggagaa taaatgaatc aaaaaaaaat
     7801 aaagattagt attaaaatgg taattatagg tatattttct gtaattattg cttttgtatt
     7861 atccagatta ttcgttgagt atattgatga attattaagg tctaattggc aatatcagct
     7921 atttgaaagt gagagttcat atataatatt tgccaattgt attatgttct cgaatatatt
     7981 attagaaata tttttaatat atatttgtag aaaatttaga aaaatctaat atggtcaacg
     8041 tatttcgaat cgaatacttc atgattattt agtctattgt gctagttaaa gtttaattcc
     8101 catataaagt aaattgattt tagtaaaaca aatcacccct aagggttagt ttatggataa
     8161 gtcatccaaa tattaactct ttaatttttg ctatagattc atctttgctc ataactttac
     8221 ttataaaaaa tgaaatatta taagctagaa cttttattct aattctagtc ataaatccta
     8281 gcatagagtt acttttttta tattataaat aaaactatac aacaaagata atatttagta
     8341 aaaataactt tcattttaac taaacttaaa attcaattac tctctaaatt gacgtttagg
     8401 aattagttca aaataaaaaa gagtgtatat attgaaatta ctacatttca atatatatac
     8461 tctttttaaa tttctcagtt ttccatggaa ctttacttta tttatttctg tattagttgt
     8521 attttttatg catttttctt taaaatcata ttttctgatt caactcttat attataaaat
     8581 ttctttacta aattattctc tttttctata tcttcatata tattttttaa tcgcctttta
     8641 cctaatcttt tatctaacat tactaatgat ttaactaata tattatatga ttttatcgca
     8701 tcttctattg agatatttaa acatatattt actgcttcat aaaaatcaaa ttcattaaaa
     8761 aatccatttt tatttacaat cttatatccc tttgcatctc tttctaaata ctcttcctaa
     8821 atactcttca aaactttcat catctaatct aaatcctata tcactatgaa aaataggttt
     8881 tgcatttgga tatagttcta ttgttttatc aaaagtttcg aataccaagt tattattatt
     8941 cgattttcca atagtccact ataaacaata ataagtttgt tgctccaaat aatgtaacac
     9001 ttgtagaaag atgttacaga tatataaaag atagagaatg tagtttttct cctaatacta
     9061 ttagaaatag aaaaaatatg attaaaaatc aaattgaagt tttctttaaa gatatgaaat
     9121 taatagatat aacaccttct attttacagt cttatataaa taacatatac aatgagcaca
     9181 tgttaaattc aataaaaaac caagtcgatt ttataaaatc agtattgaaa gaatcttata
     9241 gattaaaaga aatatcagaa aatatttgtg attttgttac aaccccaatc aaaaaagtag
     9301 ttcaacttct aagctttata caaaacaaaa agctcaatta ttattagaaa aatcaaaaaa
     9361 tattcctata ggtataccaa tttttttgat gctaactctt ggattacgtt ttggtgaagc
     9421 tgtatcactt atatggtcag atgtagacct tgataaaaaa tctcacatgt aaatcaaaca
     9481 ttagtttatg tatacaataa aataatcttc aaagacccta aaacccctat atcaaaaaga
     9541 aaattatttg ctcctgatga acttataagt ttgcttaaag gggaaaaatt aaaacaaaat
     9601 aagttaaaat tacagggtat attgaaaaat gaatttgatt tgatatgctt aaataaaaga
     9661 ttcaacccgt gagttcaacc aacttttgtt gttcatttta aaaaactatt aagagataat
     9721 aatcctagag atataaaagt acatgaatta atacatgcaa atgcaacttt gatattactt
     9781 tcaggtacta acataaaagc tatttataaa atattagtac atgcagatat aaaaatagct
     9841 atgaatatat attccatatt ctcacttatt agatgaaatg aataaagaaa catctgaaaa
     9901 tataagcaaa atactattca aataatgttc agttttatgt caattaaatg tcactcaaag
     9961 tgcttttttt tcatttgtca gttaaatgtc acttaaagta cctttttcct atttgtcagt
    10021 taaatgtcag taatatataa ataagatata tttataagac ccttaaaatc actattattt
    10081 aataaaattt ataattatat actatttgct gttgacattg gtattatacc atagtttatc
    10141 atttatatta gaaataaaat tattaattaa tagtttcgta attttataat agatttacag
    10201 ggagtgatag taaatggata caaaaatatc tataaacgaa aacatgagtc taggtaaaag
    10261 attttttaaa tacctagcac catctgtagt tgcaatgtgg gttttctcac tatatacaat
    10321 ggttgatgga atatttgtaa gtaaaggagt tggagaatta gctttagctg ctgtcaatat
    10381 atcaatgcct tttattaatt ttatatttgc agtttctcta ttattttcaa ctggtgcatc
    10441 taccataatt gcaatctatc tggggaaaaa agatattaaa tctgctaacg aagtcttttc
    10501 atttaactta gtatcaataa ttatattatc aataattata ttagctataa ctttttttaa
    10561 tttagataga ttagcactat ttttaggagc tacagaatct acaattggta tggttaaaga
    10621 ttatttagga ataataatat tttttaacgg attctttata gtgtcttatt ctttagaagt
    10681 tataataaaa actgatggct ttccaatatt agctactgtt ggtgtaataa tatctgctct
    10741 tacaaacatc atacttgatt acttgtttgt aattgaattt ggatggggtg taaaaggtgc
    10801 tggtattgca actggtttgt ctcaagtctt ttctactatt ttcttcttaa tacatttttt
    10861 aagaaaaaac tctactttga atttttcaaa atttagaatt gattttaaga cacttagaaa
    10921 aattgtattt ataggttttc ctgattcaac tactgaatta agttgtggta tagttgtttt
    10981 actttttaac ttaagtctta ctaaatatat tggagaaaat gctcttattt attatagtgt
    11041 cataaattat ataaatacat tagtacttat gactatgatg ggtataactc aaggtatgca
    11101 accacttact agcttttatt atggtgcagg aaatatagat aatgtaaaaa aattattaaa
    11161 aatggggata aaagccacaa taatagcatc agttgctgta tttgcaattt gtatggcttt
    11221 ttcaggacca atagtctctt tatttataca tccagaagaa acaatgctat tcaatgaagg
    11281 agttcgtgta tttaaaatat tctctatatc tttcttattg gtaggaatta atgtaataat
    11341 ttctggattc tttgtatcag tagaaaaacc atcaatttct actgttatat ctcttggtag
    11401 agggcttgta atagttgttt taagtctaat ttcaatgata ctaatatttg gtggacaagg
    11461 aatatggatg actactattg tttctgagtt tatatgtctt attttatcat tagtttttct
    11521 aaaaaagaat ttttctacat tagactctaa tctaaataag gttgcttaga tgataagagt
    11581 gacaatatat gaaaatatct aaataaaaaa tacctattat acaatcttgc aaatattaaa
    11641 cctttgcaaa aattaggatt atataatagg tattttattt atgtaattat tttatttcta
    11701 aatatccata gccatctttt attaaagttc cttttacttc atattctaca ggttttttaa
    11761 atattggacc atctacgaca aatgtatgat actctccatg ttctccacat gggtctgcac
    11821 cagcattttt tatcttctcc acaagttctc tagttaattt ttttcctaaa aattcctctc
    11881 ctaagtgctt taagtttact tttgttacaa cactacaaaa tccactgtct ataaattcat
    11941 atactaactc ctctctactc tcttgccata atggtaaatc tactttcata ccaactgctt
    12001 cacatctatc agtatcccat tttctatgac tttctatatc tatatctcca tatgcacata
    12061 tagttgctcc catgttttta gcttttatta aagccctctc aaagctctct tcatatcctt
    12121 ctacaccaca ctcagcaact aataaaggta atcctatatt gctacttact tgcttaagca
    12181 tattatattc taaattatgt gtccatgact ttccatgttc ttcacttata gttgtcaaga
    12241 gtgccacagg cttatatcct tttttcaaca ttcgatttaa tgctaatata ctatccttac
    12301 caccactaaa tgacattaca aatttttcat cagccaattt taacacctct ttcatctaac
    12361 tttgttttat taacataatt ataccaaatt tttgagttgc atagcaaatt atattaaatt
    12421 gttaattcac tataaagaat gtccctaata ttaaaagtat aacccctatg tatttgtata
    12481 aatgcattgt ttcttgtccc aaaattacgc tatcaataaa aaatccaact aaaacttggg
    12541 atacaagagt taatataagt gtatttgata ctcctaaatt tggaattgcc tttactgtaa
    12601 aatatattat tagacctcca aatactcctc ctaataacaa cttataatta aaaccattta
    12661 tagatactag acactttaag tcacctgtaa ctattatact tgccaaaaaa aatattgctc
    12721 ctacagttaa gcttataaag gtggccacta atgctgttgt actttttcct agctctccat
    12781 ttataaaagc ttcaagagct gtagaaccac ctgctaataa tgcaaatatt attgcccata
    12841 attttgtcat ggacaaacct ccttctatat aattctatta atagaagttt atgatacaac
    12901 ctattaatta attactaagt atagtttaat ttttatacac taggctttat ttggatatga
    12961 atttctgtta taattgttct atcgattttg gaggtgattt tatgtacaca aattttaata
    13021 tcgattttaa taactttaat aaaaaagaaa atgccattaa atttatgtta atgggagtat
    13081 tactcattat attaggatta ttatgcctta catttaagac attaggtata aaattaattt
    13141 cttggacttt tggtattgca cttttattct ttgcatattt aaatttaaaa aatattaatg
    13201 aactaaaaag atatgctact aaagaagaaa ttaaaccctc tattaatatc caatgggttc
    13261 taataattgc atgtatatta ctttttgtat ttcctcaaaa aatacaaagc atattttcat
    13321 tatttttagg attttattta atctttaatc aactagtagc acttgtaaat agtaagaata
    13381 atccttattc taaatttaca acttggaata tagttaagat tctatttggt atatgcctta
    13441 tattatctcc acttttttta tctagattta tagtatcgat aatgtctttt ttcataatat
    13501 tatttggact agttctattt ttctctggaa atacagcgag aaaatattaa ttattttctc
    13561 gctgtatttt tatattactt aaaatagaat ttgtttaagt tatctatttg tggtaatatg
    13621 ttttttgtac aagctcagcg tacaatttta ttagaaatag gtgatttttt ggataaattt
    13681 aaatatgctt ttgataataa aagatatcat acttggaatt actatcttag aaatactttt
    13741 ggagaaaaag tttttaaggt atctataaat gcaggattta cttgcccaaa cattgatgga
    13801 agtcttggtt atggcggatg tacctattgt agcaaagaag gctcaggtga ttttgctgga
    13861 aaccctaaag ataatcttat cagtcaattt tataatataa aagaaatgat gttaaaaaaa
    13921 tggcctcatg caaagtatat tggctacttt caagcctata caaatacgta tgctcctctt
    13981 gaagttttaa aagaaaaata tgagactata ttagaacttg aagatgtcat aggtctttca
    14041 atatccacaa gacctgactg tttgccagat gatgttgtag aatacttgtc tgaactaaat
    14101 aaaagaacca atttatgggt tgaacttgga cttcaaacaa ttcatgattc tacatctaaa
    14161 attataaata gaggtcatga ttataaaaca tttgttgaag gtgttgaaaa attaaagtct
    14221 aaaaatataa aggttgtcgt acacattata aatggtcttc ctggtgaaga ttataatatg
    14281 atgatggaaa cagcaaaggc agttggtaaa atgggtgtag acggtataaa aatccatcta
    14341 ttgcatgtta taaaagatac tcctatggaa aagatgttac aaaatggtat gttaactctt
    14401 atggaacaag atgaatatat caatcttgtc tgtgaccaat tagaaatact tcctgaaact
    14461 atgataatcc atagactcac tggtgatggt aagagggatg aactagtagg tcctatatgg
    14521 agcctaaaaa aatgggaaat tttaaataaa attgatgata ctttaaaggc tagaaattct
    14581 tatcagggct gtaagtttgt ttaatacaaa ataataacta tagaaatggt tatacaaata
    14641 tagtcctttc tatagttatt tagtatttac aaaacaaaat attaatttgt acatttatac
    14701 aatatataat aaaaaataga tagtctaaac tgtatctact tatattttat ttgtactgtc
    14761 cactttattc tataaaattt ataaatagtt attttttaat accaaatata atttggtata
    14821 atttttgttg gaatctacaa tgtataccaa taaatccttt ttaagtatat aattctaaat
    14881 actttcagtt ttttcaatct gctttaactt gttggactta taaaatattc ttatttcatc
    14941 ataagaaaga ttaagtgcta ctactgcaga tattaacatc aacatcactc caaataactt
    15001 ccctaaggaa aaactttctc ctaatatagt gcaaccaatt attactgaac caactaattc
    15061 tactgatgcc acaacacctg caactgacaa ctctacacct ttagatatac ctgctgcata
    15121 aaaaatatat gcaagtgcag ctggaacaat gcctaacatt agcatacaag ataaaacatc
    15181 taaatcctta gcataattta agatttccca aggtcttgaa gaaggaatca taaatatagc
    15241 tccaaataag aaactgtata ctaatattgt tgaacttgaa aattccttta aagcattttt
    15301 actaatagtt ggcataagtg catatgctat tgctgataat actccaagta aaagcccaaa
    15361 agcgtttaaa ccttgaaaat ccaaacgtcc accagtcact gccataattg caccaataaa
    15421 acaaagaata agtgaaaata atttgcttct agtaatattt tctttataac ataattttga
    15481 aaatattgct aaaaacagtg gtgatgtata tagtaaaact gctgcaattg atacacccgc
    15541 aatgtcaatt gctttaaaat aacataaatt aaacattgcc tgacatataa ttcctattat
    15601 tactgaatat ataattcctt ttttgtttat ctttaagatt tgtggtgtcc ttatagatga
    15661 atatacgcct aaaactataa atccaaaaaa caatcttgta aatgctactt gttctgatgt
    15721 cagtccattt cccattaaaa actttccaaa taatccaagt gtagcccata ataaccctgc
    15781 tattgctata aatacatacc ctttactttt ttctcccatt ttaaattccc ttctattttt
    15841 atttagtcaa tttacactct tctttttaag caaaaactat gccatcttaa atcaatatat
    15901 atcgactcta aaaccctagt ctttccaata atactaataa attttcaatt tttttatatt
    15961 tatttttcgc ttactttaag ttgatttaga cttattttaa ttgtaaaatg agttatatat
    16021 aacttattta aagttaaaat agactgaaac ttatatataa atatcaaatt ttaagtatat
    16081 taatcaatta aacctatttt aatatatcat taaaaattta ggaggaaaat ataaaaatga
    16141 atttagattt ggaattttat caaaaaatat tagaagcttc ccatgatgaa atatgtgttt
    16201 ctgatgataa aggaataata atatattgta ataaagcatt tgaagaaaat tatggtctaa
    16261 aaaaagaaga tattttggga aaaaatgttt cttttttaga agactctgga tattcaacaa
    16321 aaagccccat accaattgta ttaaaaacaa agactaaatt ttcactggaa caagatactc
    16381 aaacagggaa aaagcttatc ataactgcta cccctatatt tgatgaaaat ggacacttag
    16441 aatttactgt tgaaaattgt agagatataa ctgaacttaa taatataaaa aataaacttg
    16501 aagacacaaa aaagcaagta aaaaaatata aatctgaagt agaaacacta tacagaactg
    16561 cacttagaat tgaagatact gtaattatgg acggaatagt tatgagacca atcataaaca
    16621 cagtaaatca tgtttctaaa acggatgtaa gtgtactttt attgggtgaa tctggaacag
    16681 gaaaaagctc acttgctaga tatatacacc acaacagcaa tagatctaat ggcccattta
    16741 taacaataaa ttgtgcaact atatctccac aacttcttga atctgaactt tttggatata
    16801 cttcaggtgc ttttacaggt gcaagtacta aaggtaaagt tgggctcgta gaattagcta
    16861 atggaggaac tcttttttta gatgaaatag gagatatacc acaaaatcta caggcaaaat
    16921 ttttgcaatt aatacaggat agaactttta ctccagttgg gagcttaaaa aataaaaagg
    16981 tcgatataag aattatatct gctacaaatg ttgatttagt ttcaaaagta aaagaaaaaa
    17041 agtttagaga agatttatac tatagattaa atgttataga aataaaattg cctcctctta
    17101 gagaaagaag ggataatcta gtggaaatta taaaatatta ttttaatagg tactcaagtg
    17161 acttcaacct aaataaaact atttctaagg aagctatgga tgctattgca aattatagat
    17221 tcccaggcaa tataagagaa ttacaaaata taattcaaaa aatcttgctt acttgtacag
    17281 ataatcatat aacaatagat gacttaccaa acattcttac aaaaaatata catattacca
    17341 ataatggaaa taaaacacat atatcccaaa ttaataaggt tattatatct gactcaaaat
    17401 ctactaatta taaaaataaa aattttgaca ctttaattaa agagtatgaa aaaaatataa
    17461 ttttggatgc ttatgaaaaa tttggtagtt cttataaagt agccaaacac cttgaaatct
    17521 cacagtctaa agccaatagg ttaattagaa agtatactaa tacttaattt taaatttgat
    17581 aaataaaaac ttaacactat ttacatattg taaatttggc catttactgt tattatgaat
    17641 atggttgaaa ttatttaaat aaatactgtt gacttagttt catatattta ccattaaact
    17701 tttatcataa atatcatatc ataataatat atttaataag tctaataaac tatacgaggt
    17761 gtaaatatgt taatgaatac ccattttatt atggcaaaat caatacttga taatatagat
    17821 gaaaataaaa cattctttat aagcgaaaaa aattttatat atggcaatat aaagcctgat
    17881 gctttttcta aatataaatt aaaaaaacat tatttagatg agtcatttaa tatgattgtt
    17941 tctaaaataa actatctatg cagtcttaat atagatagtc tttctaaata cttttcagta
    18001 agtagactta gtcaggaact aggagtcata tgtcattttt tatgtgattt tttctgtgta
    18061 gctcacagtg aacgttggga atttaagcat agcttaaata aacatgttac ttatgaaaga
    18121 gaacttggag catttgcaaa agatgttgat ttatctaaaa ttaaatgtgg tactataaat
    18181 agtagttttg agaagttttt tactaaactt tatagtgatt acaaaaagaa aactgattat
    18241 atgaatgatt taaaattttc agcttatact tgtaatagtg tcataaatta tatacttgac
    18301 tctattttat ctaatacaat aaaatcatat catatagcaa attgcggtta acttaagaat
    18361 ttttaaactt atataaactt ataattgaat ataattaaaa tgactgctct atagaatata
    18421 attttatact tctctaggac agtcattttt tatactaact atttatctcc attataacat
    18481 ctatggcact ttttacacta tctactacat aatttgcact ttgctttaca tcttcccttg
    18541 ccttagacat tgcaaaacta tatttaaatc cttctaacat gcaaatatca tttggagaat
    18601 ccccaatagt agcaacttca tctggactta catttaacgt ttttattaca tgctctatac
    18661 ttcctctttt tgacacactt ttaggaacta tatttaaagt atatgttcca gaaaaatata
    18721 tttctaagac ttcactaaac tcacttttta gataaagttc tattctggat aactcttcca
    18781 aattttcaga acatacgcct atatttccta taagattatc aaaactactt cttttttcaa
    18841 aatctgggtc aactataaag tcactagaat aatgtctaaa agcagtggta tcactaccca
    18901 caattacatc acccttatat ttaaaataaa gttgtgcatc acttgactca aatctatcaa
    18961 tgactttttt tactaaatta tcatctatcg ttcttttaaa aattacatta taatctttat
    19021 catatataaa tgaaccattt tcacatacat agtatccatt catatctaaa ctatctttga
    19081 taatattatg tgcttgttta aaaattctac ctgtagcaac attaaactgt actccctttt
    19141 tttctatttc ttcaattgct actatatctt cttttgttat ggttccattt tcatatagag
    19201 ttccatctaa atcacaaaat atatgtttta tcataaatta aaagcctcca aaaacaattt
    19261 tatattttac taatacttta atcaaaataa cttaaagctt ttctaattgt attctcaatt
    19321 cattaagcaa atctctagta gctcttaatc ttatctttct tctatcccca ttaaatatat
    19381 ttttctttac tattgttttt ccatttatat atattgcagt ataaaccagt cccacaggct
    19441 tctctttggt tcctccacca ggtccagcta ctccagttgt agatatacct atattagctt
    19501 taagtccttt agcaatgccc tcagccattt ctatagctgt ttgctcactt actgctccaa
    19561 actcttccaa agtttctctt tttactccta atcttttcat tttagaatca ttagaatatg
    19621 ttacacaacc ttcaagaaat acagaggata tacctggata gtttataaga gatgaagaaa
    19681 ccaaacctcc agtacaagat tcagccactg ctattgttag atttttttct acaagtagct
    19741 tagaaacagc atcttctaaa gcagtctccc cttcagatat ggaaatatct ccttcagtat
    19801 atacatattt accaactcta ctttttattt tttcttctac tggttttatt aatttaaaag
    19861 cttctctttc attttcagcc tttgcagtta ttcttattgt aacttctaat tcttttgcat
    19921 ataaagcaac tgttggatta gtctgctcat taattatatc gagaatttct aattctagat
    19981 ttgactcacc tattccatat aatcttaaag tcttagatac tagcatttca tttgtgaatt
    20041 gttgtaagta aggtttgaca ctctcattaa acatagcttt catttctctt ggaggtccag
    20101 gcaatactat tataaattta ccatcttttt ttaaaattgc ccctggagct gtaccattat
    20161 tattttttag tactatagca tcttttggaa agtacacttg tttcatatta ttttcggcca
    20221 tttttttccc tgttttaaca aagaaagatt ccaaaacctc tagtgaaggt ttatgtagtt
    20281 ctaatttctg accaaagtat tctgcagcag tttcttttgt catatcatca ccagttggcc
    20341 caagaccacc tgtagtaatt acaaaatcac ttctctttaa gctttcatca aaacattcta
    20401 aaagtctttg tttgttatct ccaacagtac tctgatggta tacttctatt cccaatgatg
    20461 caagttcttt agctaaaaat tgagaatttg tatttactat atctcccaat aatatttctg
    20521 ttcctacact tattatttca gctttcaaaa tttatcactc cttgatttat agccattaag
    20581 actagtctta agttcataac aataatttta gcaaaaataa taacataaat aaagttaaaa
    20641 aatcatttaa acatatcttt aaactagatt attattc
//
LOCUS       DAELTA010000063        20618 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.063, whole genome shotgun sequence.
ACCESSION   DAELTA010000063 DAELTA010000000
VERSION     DAELTA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.063"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(37..711)
                     /locus_tag="KPG13_001918"
     CDS             complement(37..711)
                     /locus_tag="KPG13_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG0985651.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(714..1766)
                     /locus_tag="KPG13_001919"
     CDS             complement(714..1766)
                     /locus_tag="KPG13_001919"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG0985652.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(1815..2774)
                     /locus_tag="KPG13_001920"
     CDS             complement(1815..2774)
                     /locus_tag="KPG13_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG0985653.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(2867..3388)
                     /locus_tag="KPG13_001921"
     CDS             complement(2867..3388)
                     /locus_tag="KPG13_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG0985654.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(3399..4733)
                     /gene="celB"
                     /locus_tag="KPG13_001922"
     CDS             complement(3399..4733)
                     /gene="celB"
                     /locus_tag="KPG13_001922"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG0985655.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(4772..5098)
                     /locus_tag="KPG13_001923"
     CDS             complement(4772..5098)
                     /locus_tag="KPG13_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985656.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(5122..5430)
                     /locus_tag="KPG13_001924"
     CDS             complement(5122..5430)
                     /locus_tag="KPG13_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG0985657.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(5571..7484)
                     /locus_tag="KPG13_001925"
     CDS             complement(5571..7484)
                     /locus_tag="KPG13_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0985658.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(7816..8598)
                     /locus_tag="KPG13_001926"
     CDS             complement(7816..8598)
                     /locus_tag="KPG13_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG0985659.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(8601..10820)
                     /locus_tag="KPG13_001927"
     CDS             complement(8601..10820)
                     /locus_tag="KPG13_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG0985660.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(11084..12181)
                     /locus_tag="KPG13_001928"
     CDS             complement(11084..12181)
                     /locus_tag="KPG13_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985661.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(12496..13689)
                     /locus_tag="KPG13_001929"
     CDS             complement(12496..13689)
                     /locus_tag="KPG13_001929"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG0985662.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(13826..13929)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            14118..14627
                     /gene="yyaC"
                     /locus_tag="KPG13_001930"
     CDS             14118..14627
                     /gene="yyaC"
                     /locus_tag="KPG13_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG0985663.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(14738..15166)
                     /gene="fabZ"
                     /locus_tag="KPG13_001931"
     CDS             complement(14738..15166)
                     /gene="fabZ"
                     /locus_tag="KPG13_001931"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG0985664.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(15182..16201)
                     /locus_tag="KPG13_001932"
     CDS             complement(15182..16201)
                     /locus_tag="KPG13_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG0985665.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(16398..16658)
                     /gene="spoIIID"
                     /locus_tag="KPG13_001933"
     CDS             complement(16398..16658)
                     /gene="spoIIID"
                     /locus_tag="KPG13_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG0985666.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(16756..17424)
                     /locus_tag="KPG13_001934"
     CDS             complement(16756..17424)
                     /locus_tag="KPG13_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG0985667.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(17490..18554)
                     /gene="spoIID"
                     /locus_tag="KPG13_001935"
     CDS             complement(17490..18554)
                     /gene="spoIID"
                     /locus_tag="KPG13_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG0985668.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(18641..19894)
                     /gene="murA"
                     /locus_tag="KPG13_001936"
     CDS             complement(18641..19894)
                     /gene="murA"
                     /locus_tag="KPG13_001936"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG0985669.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(19915..20601)
                     /locus_tag="KPG13_001937"
     CDS             complement(19915..20601)
                     /locus_tag="KPG13_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG0985670.1"
                     /translation="MTISYADTSINTRYNRLSKALEISEANFKFYNVKINSVINHNLS
                     NKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKGSDKEISIKGINKNNKES
                     YIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVGEYTKRLQNNKYDDILQK
                     ILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKINLNIGIKYNEDDGKTLIY
                     IATPIIKLDY"
BASE COUNT         6255 a         3850 c         2158 g         8355 t
ORIGIN      
        1 tctctttatt taaactaagt attttatatt catatactat cttgaaatta tatttacgag
       61 ttgtttaagc ttttgtctat ctatatcttc aaaaggactg ttatttattc taatcgctgt
      121 accaaaatga aaattacttg ccttagttaa ttcctttatc ttttctatat tattaaaatt
      181 aagacctcca cctaaaagaa tttttatgtg attagaacta agcatcatat tctttatcat
      241 ttgtatgtta tcaactatac ttcctttccc tcctgatgtt aatatattcg taattttatc
      301 ataatccttc aaaattttaa cactatctat tgtatttgat tcatctattg ctctatgaaa
      361 tgtaacatca agattatcac aacatttcaa taaaacattt agatttttct catctatact
      421 attgttttta tccaatacac caaatacaac tccattagca ccaatctctt taaccacact
      481 tatatctttt tgcattatac ttatatcttc tttactgtat acaaaacttt tcgcatgatg
      541 tcttatcatt acgttcacag gtatctttac actgttaaca acactttcta taagaccaaa
      601 acttggtgtc aatccacctt cagttaaagc actaactaac tctattctat ctgcaccaca
      661 atcttctata attttagcgt cttctactga cattcctatt atttctaaca taactactcc
      721 tttgctaatt catatattgc atttgcaaat atctttgcat ttaatattat atcttctata
      781 tccatgtatt catcaggatt atgagctatt cctttttgtc ctggaaatga aggtccaaac
      841 ggaactatat ttggcattac ttttgcatac gtacctccat tagttgttac gggagttcca
      901 tctaatgatg taaccctttc ataagctagc tgaagctttt taacaagatt actctcttta
      961 tcaaaatata caggattaaa attagaaatt acctctatat cgatactatt tggaaaacta
     1021 gcttttattt catccaatac atctttcaat ttataactaa aatcgtattt tacctttata
     1081 tctaatatag tatcactatt ttctatattc agctctttat atgtaacaag cacattttca
     1141 gacatatttt ctataaaacc taggtataca tcttcttcat aattgatttt actttttatt
     1201 ctaatctttg ccatgccttt ttcaccatat acaacaggat atttacaatc tggtgtaaat
     1261 cccataatag gtgctttttc tttttctaaa taatatggta tatcttcaaa tccactttct
     1321 tcatttgtac caaatattat tctaactctc ttatctaatt ttaagtttaa ttctttaata
     1381 gcatataatc catataaggc agacattata ggtcctttat tgtctagtac tcctcgtcca
     1441 taaattcttc cattagtttc ttctccctta taaggctgat gcttccaacc atctccttca
     1501 tgtacaacat ctacatgtcc aattacacaa acataatcat cactatctcc atgctctgca
     1561 taaccaatat aattatccaa gtttctaacc ttaaatccca atttttctga tatttctaat
     1621 gctttattaa gtgcctcccc tactttttcg ccaaaaggga agccattttc tgactcccct
     1681 ttgacactag gtatttttac aatatctata atatcactta ataaatcttc ttttaactca
     1741 tctattttat tacttatgat attcataaaa atctccttat tataaaactc tattttaatt
     1801 atgtaaccac tactttactc acttacttct attggtgctt ttattctagc ctcatatgct
     1861 tccatccact catttgatat aaccttatgt atagtctttc catctactct atttgaaata
     1921 tatacagtag gagattcttc tttagttact actgaaaaag taaaatcagc tatatttgta
     1981 gctactcccc attcaccttt attattcatg gcaactaatg ataaatcacc agcttttcct
     2041 cttcttttga taagctgttt atcaagttca tttactgcaa tatcacatgc ttcttgtgga
     2101 tgcttccctt ctttcataag tctaactatc tcataagata tacatccctt cattaaatct
     2161 tctcccaaac cagtagctgt agctcctcct atactactat caacatagaa tcctgaacca
     2221 gatagtgcag aatctcctat tctacctctt ttcttcataa acaatccact tgttgatgta
     2281 gctgcacaca tttttccagt tttgtctaaa gatatcatac caacagtatc atgaccagca
     2341 tatgggctta atcctttgtc taaagtctct ttttttctct ttttataatg taacttcgct
     2401 ctatcagtca acatattttt tctttcaaat ccatttttat gagcataatc ttcagcacct
     2461 attccaacta aaaaattatt taccttctca taacttaact tcctagctat gctgacagga
     2521 ttagaataat ctcttatacc agcaactgcg ccaatatcta atgtgttacc atccataaat
     2581 gcagcgtcaa gttctacctc acaattttca tttggaagtc caccatatcc aaccgattta
     2641 tagaatggat agtcttcaac tcttcttaca gcaagctcta cagcatcacc tgcttccatg
     2701 cctttttcta aatgttctga tgcctcagtt attccttcta gggccattct ccatgtcgct
     2761 atcattcccc acatgttaaa attcccctcc ttaaattata tagattttac taatgtatgc
     2821 ttattataaa tagtataata tttaaacttg ctaataatta atactcttat tgataacaaa
     2881 actcttcttt catcaaagtt atatcttctt tatttgctat tgcttttgcc aatttacata
     2941 tgttatttaa tgaatcatta agaccaattc cacctttttt tggagtctta actatacata
     3001 tgtcattttt atattcgttt atcacatcta tattttcttc tgacatagct gcaaaagctg
     3061 gtatatctgt tttatcattt atatttttag acagtattgc tgacatctta gaaaagtttt
     3121 cgtaattaaa gcttggtcca cagattacta catcagggtt taattttttt accatagcta
     3181 tcatcttact cgttacttct tcttgattat ttacaaaaaa ttcatcccca caaaataagg
     3241 tagctattac cttagattca tttaaaaatt gctccatcat tacacctggt cctaatgggc
     3301 ttgattttcc agcaggaggt atattacttt tctcttttcc tccagctcca gcttgtattt
     3361 ggtccaatat cataactatt ttcttcataa tcttcctcct ataatactaa atcatcaaaa
     3421 gatatatcat catcttcctc ttcaacagtt tcattttctt ctctcaagta ttgcttatct
     3481 agcattttta taaatggtat atacataaat attcctagta taagaagtac taattgtaag
     3541 actgcacctt gccatcctgt tgttaaaaaa ccagagaata taattggagt tgtccacggt
     3601 aactgaactc catttgtaag aggcactaat cctgctgaca tacagaagta agctattatt
     3661 atatttattg ttggaactat tgcaaatggt atcaatagta ttgggttaag cattattggt
     3721 agaccaaata tcaatggctc atttatacca aatattcctg gtaaaattga tagttttccc
     3781 attgacttta tacgtttact tttacatatt aataacatag ctatgactaa tgatagtgta
     3841 ctacctgctc caccaaatgt cgcaaacata tcttggaatt gacctgttat tatgtttggt
     3901 atttttgaac cagcttgaaa agcagctaag ttttcagctg atagaatttt taatattgga
     3961 ttatacacag ccccaacaac actacttcca tttattccaa agaaccagaa taaatgtaag
     4021 aatatataag cgattgccat agcacctaaa ctatttccta attttaataa tggcatttgt
     4081 aaaaacttaa atataaattc atgtatactt ccatatggtg tagcttcaaa agctatctta
     4141 actaaaaaga aaactaccat aactattgca cttggtatta atgctgcaaa tgattttgat
     4201 acagttggag gaactccatc tggcattttc aatgtccatc ctttttttac aactgcagca
     4261 aataatttta cagaggttat tgctgttatc atacctacaa acattccctt agaacccatc
     4321 caacctagtg gtatacctgt taccatgtaa actgattctg ttccttctgg tgtatatggt
     4381 attacaaatg gtgttattat aaaaaaggca actattgcaa ctgctgctga tgctattggg
     4441 tctacatcta tttgctttgc ataagaatat ccaatcccta aaactgcaag tagagtcatt
     4501 acttcaaatg ttgctacagt tggttgttgc aattttacag cccaatctgg accaaaaatt
     4561 tgacctacaa attcattcca tccttttata ggaaaatttg ctactaataa aaataatgaa
     4621 cctattatta ataatggtga tgatactaaa aaaccatctc taattgcaat taataattta
     4681 tttttaccta ttttttcagc caatggcata agtattcttt ccaatctatt catctatcca
     4741 ccctcatttc ctattatttt ttactataat tctacgcttg tttagatttt attagcttta
     4801 tagcaagttt taatactttt tccccattca tagttccata atcaacttca ttgattatat
     4861 ctataagttt tccttgagct tcaaactttg gttttaactc ctttagcatg tatttaactt
     4921 gtggtcctaa taatatacaa tctggatttt tttcttcaac tattttgtca atttctgcta
     4981 tcggaaatgc ctctacttct attggtaaat tgtgttcatc tgctaccttc tgcattttat
     5041 ctgcaagtaa acttgttgac ataccaaagc tacaaaataa atatactttt cttttcattt
     5101 tttaaatccc ccttaatttt tttattttaa tttttgaatt tcttttctta acataatcat
     5161 ttcttttata aggtttctct cactcataca agtcattaaa tggtcttgag agtgaatcaa
     5221 taaaatagat atatctgatt tatctccccc agcttccttt tgtataagtt cagtttgtac
     5281 tttatgtgct tttaaaattt cttcatttgc attttcaatt aattcatctg cttttttaaa
     5341 atcgttttcc tttgcatagt caagtgcttc ataaagcata gattttgcat ttccagcatg
     5401 aattataatc tttaaaacta tttcttccat tttttcctcc taagttgctt ctctactttt
     5461 agattattta taccttacaa acatataata tcttataaaa acaaaaaaac atgcacaact
     5521 ttttccatca catggaaaat tttgtgctta tccatagttc actatgaatt ttatataaga
     5581 atatctttta ccttttcaat gtcacattta cttagaatat ctagcatatt ttcatcctct
     5641 gttaaattag atatttcttt aaataattct ctaaatactt ttctatcatc atctttttta
     5701 tacgctaaca aaagtataat ctttatatcc tttccatttc taatttttat tggtgttttt
     5761 aaccttgcaa aacataaaac agattgtttt atatatttca tgtctccatg agggaataat
     5821 attccttttc caacattggt tggatatgac ttttctcttg ttataataga ttgtaaatac
     5881 tctttttctg cataatgttt ttcaattaat aaattagtta aatatgttat agcttcataa
     5941 atatcatcat gttctaactc tcttattaaa taatcagaca tcattaatct gttcataagt
     6001 ttatatgaag tgtctttttc ttttattttg tttgtgaact ctttaagttt attctcatcc
     6061 tcatcagtaa ataatggtgt tgttataaaa attggttttt ggatattttc atactcttga
     6121 aatgcaatta taaaatctat agtcttatta aagttatctc tttttaaatc ttcctctctt
     6181 aatgtttcaa taatttttat gttatcaaat ctcttttcta tctttacttt taatagcata
     6241 cttactccaa aactaaatgg acatacaata cttgcctttt tatttgattc ccttttgctg
     6301 tattttcttt ctagagaggt ttgaaagtga agtgtaagat aaccaatttc atcttccgtt
     6361 atttgttttc cttttaattc aatttctatt gaacttgata ttactgtaaa taaaaaacaa
     6421 aatttagttt ttatgttatc taaaagtgga ttttctaagt aaacattgct tttcatttga
     6481 tgcattgtaa cattcaaatg acatattaat tgttcataaa gtaatatatc attattaaaa
     6541 tttattcctg attcttctga tactaaattt ataatttttt ttgtataact tgctaattct
     6601 tcatttagat ataaagattt cttaatatta ttactattta tttgtttgtt catacccaat
     6661 attaaagatt ctataaacaa tttttcttca taatttaata ctatggataa attagatttc
     6721 aattcatctt ctatatccaa tataagcatt tttagcttaa gctctttctt agtatttgat
     6781 acttttaaag aatttcccag ccttattcgt attatattta taagcaaaaa tagcatcaat
     6841 tgcttgaatg atatatctgt aagtcttata tccatatcct cttctatagt aagcattatg
     6901 ttttttacaa ttattatatc tactatatgg aagtactcta tttcatctat gtttaatctg
     6961 tatttatcta tatatttaaa taagaatgat actaacatta ttcttatgtc tttctcatcc
     7021 ccttcaatat atataccact gcctttttta gtcgagatat ttaaattata attatttaat
     7081 gctactgata atttacttat ttcttctctg acaatatttt tatttatgta caacttatta
     7141 gataaatcat ttaaagtaac ttttttatta ttacaaagta gtaaatttag tatctcaact
     7201 tgcttatgaa gctcactatt tactctttta acttcacctc gcaacttctg atttaccagt
     7261 attttgtctg ttccatctcc actaaattta atcccaatat taggttttct tttgatataa
     7321 gcattacaaa aagtagataa ccattcatca atatatttgc aatcatttct tattgtcctt
     7381 tctgaacact caaaatcatt ggcaatctta ttaacattta taaactcttc ttttaatagc
     7441 aatgttgtta ataaattttc ttgtctttta ttcaaacata tcacctcttt atgactcaac
     7501 ctaaatttat tatatataaa aaagagctat acataaaggt atgctagctc tcttttaata
     7561 tgatatttag ctgacctggt ttttgacccc acactcgcct actggagggt tcgcttgggt
     7621 tcgccgcact actactctct ctcgatttta ttatcggtag gtcttacttc taagccgcac
     7681 catgatcatt aacccaattt ttagtgttaa cttacgtgga tccctttgcc tgcgactggc
     7741 ttggactttc aaccacagac ctaaatatca cttattcatt ttaatatttt aaactaaaat
     7801 tgtcaatata taagattatt ccataatata attatcattt gcttttgtaa gtagagtcaa
     7861 tacaaaaacc ttattagcac ctgataactt taaaagttta gaaatttcgt ttgttgttag
     7921 accagtagta aatatatcat ctattaaaag tatattttta ttatttatta atttaacatt
     7981 ttctttaaca acaaatacat tttttaattc ttcttttcgt tctttcttat tcaatttata
     8041 tagcattctt gtatattttt ttctacttat gcaatctaat aatggaatat ttactatttt
     8101 acttaaatta aatgctattt tttgtgcctg attaaaacct cttttattta gccttttttt
     8161 atgaagtgga acaaataata tataatcaaa ttttatgttt tctaaatata atttttcttt
     8221 cattatttga gctatatact tagccataaa ggttttttgg ttatacttca gccctaacac
     8281 catctttttt gtcacatcat tgtattcaat acaagatatt gatttatcaa aataaaagtc
     8341 tttattaaaa caataactac actcttcaat aaattctttt tcaattgaat gacgtattat
     8401 tggttttcca cacttcatgc aaccatcttg tataaaattc atttctttaa aacaactttt
     8461 acatattgaa taagtatttg ttttctttat ggatttatca caaattatgc aacttatatt
     8521 ctcaggatat ataaaatcca aacatttatt aatcatattt ttaaaattct taataaaatt
     8581 atcattgcat attttcatat ttattctatc aacaaccctt cttgtttaaa tttatttagc
     8641 ttatatccta aatttgaata cctttgatta actctattat tttttatcat atattcaaga
     8701 tatttcacat ctccaactaa tacaactaat ttttttgctc ttgttactgc tgtatataat
     8761 aaattacggc taagtaacat aggaggagcc cacgctattg gcaatactac aactggaaat
     8821 tcacttccct ggcttttatg tattgttgta caaaaactat gatcaatttc atctaattca
     8881 tcatataggt atgatacaat tttagtctgg tcaaataata tataaatagt tttcttatct
     8941 ttatctatgt gataaacata tcctatatct ccattataga taccttctcc actttctttt
     9001 tggtcttctg tttcccattt ttttgtataa ttatttttta cttgcattac cttatcgcca
     9061 actctaaata atcttttaga aaaattttct tcaactttaa atttttcttt tttgttaaga
     9121 tacttctgta actctatatt tagattagta actcccaatt caccttttct cattgaagat
     9181 aaaacttgta tatcttttaa tttatcaact ttataaaatt taggtaatct ttcattaacc
     9241 aatcctatta tttcatttaa aatttcttca ttagttgatt ttcttataaa aaagaaatct
     9301 ttaccttttg tatttagata aagtggttct ccattgttta tcttatgggc atttacaatt
     9361 atcatacttt cttgcgcttg tctaaatatt tcatttaatt taactacatt tatgatatta
     9421 gaattaatca tatccttaag tacatttcca gcacctacag aaggtaattg gtcactatct
     9481 ccaactaaaa ttactcttgt tcctaacttt atagctctaa gtaaattgta cattaagata
     9541 atgtctacca ttgatacctc atcaacaatt attacatctg aatttattgg gtcttcttca
     9601 tccttaaaaa aagttaaatc atcatctgtt gaaaatccca tttctagcaa cctatgtata
     9661 gtttttgctt ctttattaga agtttcactc attctctttg ctgctcttcc cgttggagct
     9721 gccagagtca cacttttacc attattttca aatatatcta tgatagcatt aatggtagtt
     9781 gttttaccag ttcctggtcc accagttata attaatactc cactattaac agattctttg
     9841 actgctaaaa tttgtttttc tgctaactta attttcttat cttcttctaa aacattaatc
     9901 tcgttatcta tatctatttt taagtcttca aattcatact gtgataattt tactatttga
     9961 ctacatactc cattttcagc taaatagtat ggcataaggt atataagatt ttcattgttt
    10021 actttctcta tatgtatttt ttgattataa actagcatca ttacacactc ttttattatt
    10081 tctccattta gctccaataa tttttccgaa tcttgtatta gaacatgctc tggtaaatat
    10141 gtatgcccat tacttaaaga tttatttaaa gtgtataaga ttccctgaca aattctatct
    10201 ttagagtttt tatctatacc aattttactt gctatactat cagctacttt aaatccaatt
    10261 cctcttatat cttcagcaag ttggtatggg tttttattta taacttctat tgcactgctt
    10321 ttatattttt tatatatttt taggcagtaa tttggtgtta ttccaaaagg agatagctgt
    10381 attattatat ttctcaattc tctgtcttct tcataacttt ttactatttg ttttactttt
    10441 ttactcccta taccttctac ttcttgtagt ttttctggtg aattttgtat cacctctaac
    10501 gtatctacac caaacttatc aattattctt tttgccattt tttcaccaat accatgtatc
    10561 attccagaag ataagtaaac ataaattcct tctaaggatg atggtgttac tggcataaaa
    10621 ctattcactt caaattgagt cccataaatc ttatgattta cccactttcc ttcaacttct
    10681 atgctttctc caattgctaa agtaggcata catcctacaa ccacaatttc atcattttca
    10741 tttaccaggt gagctattgt ataaccatta tcttcgtttt taaagactat ttcacttatc
    10801 attccttcta atttttccat ttttatctcc taaaaatact aataatcgct ccatttaaat
    10861 gttagtataa ttttatatat taaaactatg ataatcgtag aaaagtttta tatttttata
    10921 cttaacgtaa tttccaacta ttatacattt tatcagaaac aaaaaaaatc tgcatccata
    10981 aaatatggta ccatgaaata caggtaccat atttaaatag ctattttatt ttaattactc
    11041 tatatttaga gtataaatta aatttcataa gtatattaca gtactaactt atttcagatt
    11101 tattctcctt gtacccctca gttttagtaa ttcttctata aagcatacat agtaaaatta
    11161 aaattcccat gtaacctgta tatggatata gtgtacctac taacttatca aatggcagta
    11221 aacctcctat acaagctaat atacttacca ctattgttat aattttaaac ttagggtggt
    11281 catcttctac aaatcggttt gttactgacc ataacaatgg tacagctgta gtgtatattc
    11341 ctagtaaaag tactactgag aataaaatac ctattattgg agatatctta tcagctaagt
    11401 aaagtgctgg tatctctttt gtgtatatat ttcctatgtc tgaaagtaat gccaaattca
    11461 tcattatagc tgctgccata agtgctactc cacctactat accaccccat acagcatctt
    11521 tcttatttac tgcagaagct cccattcctg tcaagaatgt tattactatt attacattgt
    11581 aacaagtata aagtactccc gagaaatatc catttgatgt agcagtttta atatttaaac
    11641 tatcaaccat atttccagca tttgataaag catcaatatt gctaaataaa ctaattgctc
    11701 ctactaaaag tgtaaatatt attattatag gtcctatatt acctagtatt ttagaaagcc
    11761 ttgtcaaacc taatgaaaca gttattaggg aaacgattgc cataccaatc ctacctacat
    11821 atggatttaa tccatagtac tctgataatg tagctcctgc tcctgcaatc attattacta
    11881 atgttccaaa taaaaacaat ggaccaaacc attcaaaaaa tgttccaaga tattttccac
    11941 aatatacttg ataaattttt attggctcct taagtttcaa ttctcttccc ttatctataa
    12001 cttctgctcc aaaccatgaa aatagtacca tagagattat tccacctata atacccgcat
    12061 atccataaaa cgtaaaaaat tgcagtattt cttgaccagt tgcaaatcca gaacctatta
    12121 cagtagcaac ataagctcca gcaaaactta taaccgtttt aatatttact ttgcctgaca
    12181 tacactactc cccccaatta aaattatttt taaagtgtaa gtttattaag attatattag
    12241 actcattatt gtgatatttc aatatatttt taattattta acattagtta acacattctt
    12301 aaccatcctt taacatatat cccataatat gtataaattt agtagagtat tatataaaat
    12361 gtattgacca gattaaatac tgtttattca ttatatgatg ataaattaac atttattaaa
    12421 aatatttaac tcataataaa aaagctgtta gaaagtaatc gtaaaattac attctaacag
    12481 ctttttagtt aaaaattatt cacctaaagc atcttttctt aactgctcaa ccttgtcagt
    12541 tctttcccaa ggtaaatcta tatcagttct accaaagtga ccataagcag caaccttttt
    12601 ataaagaggc tttcttaaat ctaagtctct tattatcgct cccggtctta aatcaaagtg
    12661 tttatttact aattcaacaa gtttttcttc tgaaaccttg cctgtcccaa aagtatctat
    12721 aaatatagat aatggtctag ctatacctat agcatatgca agttctatct cacatttgtc
    12781 tgcaagacca gccgcaacaa tattttttgc aacatatcta gctgcgtaag cagcagatct
    12841 atcaactttt gtagggtctt ttcctgagaa agctcctcca ccatgtctag aatatccacc
    12901 ataagtatct attattattt ttcttcctgt aagacctgta tctccttgtg gtcctcctat
    12961 aacgaatctt cctgttggat ttatataaat tttagtctct tcatcaagta attctgctgg
    13021 tataacttcc ttaataacat gattaattaa atcttctctt attttatcat ttgatactgt
    13081 ttcacaatgt tgagctgata taagaactgt atgtactctt acagctttac ttccttcata
    13141 ttcaacagta acttgagttt ttccatctgg tcttaaataa tcaactaaac ctgtttttct
    13201 aacttctgta agtcttcttg ataatttatg tgccaaagat atcggaagtg gcattaattc
    13261 tggagtctca ttacatgcaa atccaaacat tataccttgg tctccagctc caactttttc
    13321 tatttcttct tctatctctt ctccagtctt actttctaga ccctcatcaa ctcccatagc
    13381 tatatctcca gattgttcat caatagaagt tataactgcg caagtatcac aatcaaatcc
    13441 atattttgct cttgtatatc caatttctct tactgtttct cttactaatt taggaatgtc
    13501 tacataagca gatgtactta tttctcctgc tactaaaact aaaccagtag taactgtagt
    13561 ttcacaagct actctagatt gtgggtcttt ttctaataat gcatctaata ttgaatctga
    13621 tatttggtca catatcttat caggatgtcc ttctgtaact gattctgacg taaataaatg
    13681 tcttgccatt tttattcccc cttatttatt cttaattttt attataggtt ttaccttgtt
    13741 tttaatttta gaaatttact caaaataaaa aaacctctcc tttataagaa gaggttaata
    13801 atcgtaatta catactaatt aactgacctc atctttcaag acttttgtct tgtaggaatt
    13861 agcacctttt cccatttggg tggttgctgg gcttcacagg gcctatccct cagcctctct
    13921 tgataaggta atcaagattt aagttttgtt ttactttact agcttactat ttttttaaaa
    13981 ttttgtcaat atttttagtc atattttttc tattcctttt tttgtaagac aaataaaatt
    14041 ttctatctat ctgaaattta aagttaggtc tttttaatgc tttttattca tatatttttt
    14101 tgaagggggt gttcctattg tatttagcta aagtaaacta cagcgatgat gatgttatac
    14161 agatgctaag ttcagttcta aaaactataa ttaatgaaaa tacgattgtt gtttgcatag
    14221 gaacagacag ggctataggt gatactttag gccctttagt tggaacaata cttaagaaca
    14281 gtaactttaa ataccccgtt tacggtacac tagataaccc tattcacgcc ttaaatatat
    14341 atgagtcttt agatactata aaaaatacac atatacaagg taacttcttg gctatagatg
    14401 cttgcttggg gtcgcagagt aacataggta atatacagat tagagaagga cctatcctgc
    14461 ccggaaaagg tgttggtaaa aaactacctc aaataggaaa ttattctata gtaggaattg
    14521 tagataaaat tgatgaaaat aataaacttt catttaataa tatacgcctt agctttatat
    14581 tagatttagc tgaaacaata gccttggctt tattagtatc cacctaatct aaatataaac
    14641 taaatgataa aattctttat ataaaaaggt agatatttta gagtatctga ttaaatactc
    14701 caaattatct acctttttac ttttatattt caagttttta tttttctact aaagcaaaca
    14761 ttaattcacc tttacagaca agttcttcac ctacatatgc cttagcatct gccttagcta
    14821 tattttttct aagagttgtc atttcaactt ccatagttaa tgtatctcct ggtcttactt
    14881 ctctcttgat tctaacttta tctatacctg caaatacacc tagttttcct ttgttttcct
    14941 ctacagacat cattgccact ccacatactt gtgctagagc ttctactatt agtactcctg
    15001 gcatcaaagg atattctgga aagtgacctt ggaaaaatgg ttcattaact gttatattct
    15061 ttataccaac agctcttttc ccaacttcta gctctgtaat cttgtctact aataaaaatg
    15121 gatatctatg tggtattaat ttttttattt catctatatt taacattcta aactctccta
    15181 attattttct tttgaaagac ccaccagttt caagtaagtc tattgaacct aaagctattc
    15241 ctgtaccttt tgcaacacaa gataatggtt catctgctat tgtaacagtt attcctgtcc
    15301 tctgttctat tattttatct aatccatata ataatgaacc tcctccagtc attattatac
    15361 cgacatctcc tatatctgct gctaactcag gaggcgtttt ttccaaaaca gcatgagtag
    15421 caactactat ttgctcaaca cattctctaa gtgcttccaa catttctgtt gaatttatag
    15481 ttatagaact aggaagtcct gtaattaaat ttcttcctgt tatcttcata tatttttctt
    15541 cttctctctt aaatgcagaa cctatttcaa acttgatttt ctctgctgtt ctttctccta
    15601 taagaagatt gtgttgtttt ttcatatatt taactatagc atcatcaaat ctatctccac
    15661 caacctttat agattcactt acaacagctc ctcctagaga tattacagct atatctacag
    15721 tacctccacc aatatctata atcatattac cattaggctg tgatatgtct acaccagctc
    15781 ctatggcagc tgctattggt tcttctatga tatatacttc tctagctcca gcttgtctcg
    15841 tagcatcttc tactgctctt ttttccactt ctgtgacacc tgttggtacg cataccataa
    15901 ttcttggcat aaaaaatctt ccaaatccct ttttatcaac tatcttatct ataaaagatt
    15961 ttaacatctt ctcagttaca tcataatctg aaattacacc atctcttaaa ggtcttatag
    16021 cgactatatt acctggtgtt ctccctatca tctttttagc ttcttctccc acagctaaaa
    16081 cagaatctgt tcttttgtct atcgccacaa cagatggttc ttctaaaacg attcccttac
    16141 cactaacata aacaagcaca tttgcagtac ctaaatctat gcctatatca gctccagcca
    16201 ttttttcaac tcctttattt ataatattag attatattat aagcctattt taatactcct
    16261 ataataaagc atatacaaaa atctcgcttt aatcaagaga aaataaatct tttgagagta
    16321 ttattatttt gctatttata attacttaat atatttcttt ttataattta attcaataaa
    16381 aaaagtaggt ttttaaccta cattttttta tgttccttct catattttaa tttcgttgct
    16441 aatccccctc taatatgtct ctctgattta tttttgtcca gcacattttt aacttctttg
    16501 gcaagggatg gatttatttc cttcaatctt tctgtaacat ccttatgaat tgtactttta
    16561 cttactccaa atgtcttggc ggtttgtctt actgtagtgt ttttttcaag tatatatttt
    16621 gctaccacta tggccctttc ctctatatga gatctcaaag attacccccc tcacataata
    16681 gctttaatga tatatatgta aaaacaccaa aaaatataac aagcttaacc aaatatgatt
    16741 aaacttgtta tactattact taattagact cattgggtct atagattttt ctccactata
    16801 cgcctctaaa tgcacatgta taccttcttc actttctatt tgagaagtac tacctacagt
    16861 acctagagat tgtccttctg ttaccttctg ttctttctta acactaacat ttttatctag
    16921 atttgaatat actactacaa tgttattgtc actttttatt ttgactgatt gtccatattc
    16981 ttcatcatca aatacatcta ctactattcc gtttaataga gattttactt ctttgccttt
    17041 agtacaacta atatctacac ctttatgagt ttcccaaaca tcaagtgttt tagaataact
    17101 aggctctttt tctgaatatc ctcttattac tttatctcca atgtaattta attttgttgt
    17161 actcgattta ttttcctctt ttgacttagc tttttctaag ttttgttttg aatccgttgc
    17221 agtagggata gcatctttct tgtctttttc aattaaatgt atttcctctt cactatcttt
    17281 attagcattt tccataattc ctttattaga agccaattta tctacattat tatttgtaaa
    17341 ccaaactcca cctactgcta ataaacaaac acatacaaac aaagataaat aaaaaccatc
    17401 tttttctaac agctttttct tcattttgca cctccaaata tatttctaca tttggattat
    17461 tgacgtgttt ttggcatttt atacaattat tagtatatat cttttatttt tgtatctgtg
    17521 tagtaatgtg ataatatatc ataatattta taaccctctt ctgccattcc ttctgctccc
    17581 cactggctca taccaacacc atgaccatat ccttttacca cgaaatctat gtatccttct
    17641 ccaaatttta tgtcaaagtt tgcagagttc aacttaaaaa cagttctaat gtcttttcca
    17701 gtcaattctt tatttcctaa ttttattttt tctacagttc cggcatcact tcttttagtt
    17761 attgaaactt gtttacttaa attattcaca tctatcacaa tagtgctata tgcccttcta
    17821 agacttttaa caaaatctgt gttacttatt ttaagtgttg acgcaaattt aggggaatac
    17881 ttatcataag gactttccac agactttaaa taaggatatt ttgcagaaaa tacttcttca
    17941 ctattttctg tttttcctga agatgtagaa aaataaagag gaagtatcgc cttatcatta
    18001 taagttatta tttgaccttt agttcctcta actgcttctt ggattttgga atatttattc
    18061 ttaatccatt cttcaccatt taatttttta agagtatcat aactcttata ttcttgacaa
    18121 tgtttataat cagtacatac tactgcattt ttatgtttac tagatttacc atgttcctgt
    18181 ttatatacta catatgttct agctgctaca gattgagctt ttaaagcttc tatatcaaac
    18241 tctgaagaca tctcaccagc tagtactcca cataaatagt tctccatgtc catcttttcg
    18301 gttttccctg ttatatgatt atatacattg ataattggtg cttttttatt tacagtttca
    18361 tagttcactg tatttttttc ttctttatta cctttgtctt caatatcact ttttttaata
    18421 gttttgttta tagatactgg gctctctggt ttttcagtta attctacatt tttataagaa
    18481 actaaagtta taagtgatgg aactaacaca gagcaagtca caaatcctaa caaaacaacc
    18541 aatgggttct tcatatactc tcctccttag cttttaaaac cttgtattta agtatatgaa
    18601 caaccctatt aatttataac tttcttctat aaaccaatta ttaatcttct actatttcga
    18661 tttgaccacc tagtgctgtt atcttcttat ctatatcaac ataacctctt tggatatggt
    18721 atatttctcc tatttgagtt tctccttctg ctataagacc gcataaaata agggctgccc
    18781 ctgctcttaa atcagtagcg tttactgctg caccgtgtag ctcatctaca ccattaacaa
    18841 ccgcacttct tccatctatt tttatattag cacccattct attaaattca gcaacatgca
    18901 taaatctgtt ttcaaaaaca gtttctataa ctacaccagt tccattggct acagtaagca
    18961 tagccataaa ttgtgcttga acatctgtag gaaatcctgg atgtggtaaa gtttttatat
    19021 ctattggttt taatactttt ggacctacaa ctcttacaga attgtccatt tctgttattt
    19081 cacaaccagc ttctcttaat ttagctacaa ctggttttaa atgctccatt aaaacatttt
    19141 caacagttat atcacctttt gtcatagctg ctgcaaccat ataagttgct gcttctattc
    19201 tgtctggaat tacattatgt tctgctcctt ttaactcttt aacacctttt atttttatag
    19261 tattagtacc tgcaccttta acatctgctc ccatttcgtt caagaagttt gctaaatcta
    19321 ctatttctgg ctcttctgct gcattctcta ttattgtagt cccttctgct aaagaagctg
    19381 ccatcattat gttttcagtt gctccaacag atgggaaatc taaatataat ttatttccta
    19441 ctaacttttc tgttgctgct tcaacaaaac catggtccat ttctatcttt gcacctaaag
    19501 ctttaaatcc ttttaaatgt aaatctatag gtcttgttcc tatagcacat cctcctggca
    19561 tagaaatttt cgtactatta aatctagcaa gcaatggtcc cataaccaaa aaagaagctc
    19621 tcatctttct tacaagctca tatggagctt cacaagtttt tatattgctt gcatcaacag
    19681 taagtgtatt ttctttatat tcaacttctg ctcctacatg tcttaataaa tctgatataa
    19741 catgaacatc tcttaaattt ggtactccat ttaatgttga ttttccgttt gctaataaag
    19801 tcgctgctat tattggtaaa actgcatttt ttgcaccatc tattttaaca ctacctttaa
    19861 gtggattact cttttttact attatcttag ccatattttt tgatctcctc ataattaata
    19921 atctaatttt ataattggtg tggcaatata tatgagtgtt tttccgtcat cctcattata
    19981 tttaattcct atatttaaat taattttttc ccctaaatat tctaattcat aatcatttaa
    20041 tactttacta tatgctgtta tagataaaaa gtttacgtct ttaacttcat ctatttgctt
    20101 agcactcata ttatataata ttttttgtaa aatatcatca tatttgttgt tttgtaacct
    20161 ttttgtgtat tctccaacaa tacaagcaga cacatctact tgagatgaat atttatttaa
    20221 cacattttct acgttactat aaataacctc tttatgttta tatactttgt tatccaatat
    20281 gtcaactact atgtaacttt ctttgttatt tttatttatt ccttttatag aaatttcttt
    20341 atcgctacct tttatagtag cacataattg tattttatcg ccagaccctt tttgattcca
    20401 atttattcct tttttatcca actttagatt gttaattatt tctataaaaa tattatgaaa
    20461 ttctttattt gataaattat gatttataac agaattaatt ttaacattat aaaatttaaa
    20521 atttgcttct gatatttcaa gagctttact taatctattg tatctagtat ttatacttgt
    20581 atctgcataa gatattgtca ttcctataat cattaata
//
LOCUS       DAELTA010000064        20570 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.064, whole genome shotgun sequence.
ACCESSION   DAELTA010000064 DAELTA010000000
VERSION     DAELTA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.064"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            88..3036
                     /locus_tag="KPG13_001938"
     CDS             88..3036
                     /locus_tag="KPG13_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985671.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3014..3478
                     /locus_tag="KPG13_001939"
     CDS             3014..3478
                     /locus_tag="KPG13_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0985672.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3591..3893
                     /locus_tag="KPG13_001940"
     CDS             3591..3893
                     /locus_tag="KPG13_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985673.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3955..5319
                     /locus_tag="KPG13_001941"
     CDS             3955..5319
                     /locus_tag="KPG13_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0985674.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            5354..5827
                     /locus_tag="KPG13_001942"
     CDS             5354..5827
                     /locus_tag="KPG13_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985675.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5999..6649
                     /locus_tag="KPG13_001943"
     CDS             5999..6649
                     /locus_tag="KPG13_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0985676.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6823..7755
                     /locus_tag="KPG13_001944"
     CDS             6823..7755
                     /locus_tag="KPG13_001944"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0985677.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8029..8742
                     /gene="ispD"
                     /locus_tag="KPG13_001945"
     CDS             8029..8742
                     /gene="ispD"
                     /locus_tag="KPG13_001945"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG0985678.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8739..9224
                     /locus_tag="KPG13_001946"
     CDS             8739..9224
                     /locus_tag="KPG13_001946"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG0985679.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9319..9527
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9591..11306
                     /locus_tag="KPG13_001947"
     CDS             9591..11306
                     /locus_tag="KPG13_001947"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0985680.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11428..12873
                     /locus_tag="KPG13_001948"
     CDS             11428..12873
                     /locus_tag="KPG13_001948"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0985681.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13311..14792
                     /locus_tag="KPG13_001949"
     CDS             13311..14792
                     /locus_tag="KPG13_001949"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG0985682.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            15013..15168
                     /locus_tag="KPG13_001950"
     CDS             15013..15168
                     /locus_tag="KPG13_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985683.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15313..16710
                     /locus_tag="KPG13_001951"
     CDS             15313..16710
                     /locus_tag="KPG13_001951"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG0985684.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16695..17108
                     /locus_tag="KPG13_001952"
     CDS             16695..17108
                     /locus_tag="KPG13_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG0985685.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17214..17972
                     /locus_tag="KPG13_001953"
     CDS             17214..17972
                     /locus_tag="KPG13_001953"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG0985686.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17985..18722
                     /gene="rlmB"
                     /locus_tag="KPG13_001954"
     CDS             17985..18722
                     /gene="rlmB"
                     /locus_tag="KPG13_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG0985687.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18725..19252
                     /locus_tag="KPG13_001955"
     CDS             18725..19252
                     /locus_tag="KPG13_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG0985688.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19317..19964
                     /gene="sigH"
                     /locus_tag="KPG13_001956"
     CDS             19317..19964
                     /gene="sigH"
                     /locus_tag="KPG13_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG0985689.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20038..>20570
                     /locus_tag="KPG13_001957"
     CDS             20038..>20570
                     /locus_tag="KPG13_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBG0985690.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         8510 a         2151 c         3850 g         6059 t
ORIGIN      
        1 ttactttata aaaaaataat aagtacaaaa aaaacaatat atggcatata agttgctata
       61 taaataataa ggagggaaca attataaatg aggcaaagaa taaaaaactt aattgaaaat
      121 gaagataaga aaaatccttt aacagatgaa gcgatttcaa gtaggttaaa tattcgaaga
      181 gaagatgtta cctttttgag aaatgagtta aaaatagaag attcaagaca aagaagaaaa
      241 gttgttttaa ttaaagctat aaaagatatt ttaaaagaag aaaaaactat aaataaaaat
      301 gatataacaa gaagattaaa cagcattggg tttacagtat caagatttac agtcatacaa
      361 tacttaaaag agattcaaga ttctggtgac ctaaaacttg ataaagagag tgatgataaa
      421 aaagaagaaa taatcaataa tagagaaaat aaagatatag catttgataa actcataggt
      481 agtagtggta gcttaagtac aattataaaa ctagccaaag cagcaattct ttatccacct
      541 catggtcttc atactattat tatggggcca actggtgttg gaaagagtga gttagcagag
      601 tgtatgtata aatttgcagt agaatcatcc agattcccta agaatgcacc atttatagtt
      661 tttaatgcag cagattattc agagaatcca cagcttttgc tatcacagtt atttggacac
      721 gttaaaggtg cttttacagg agctgatgag aacaaagaag gattagtatc aaaggcagat
      781 ggaggaatat tgtttataga tgaaatacat agattacctc atgaaggtca agaaatacta
      841 tttcaactga ttgataaagg tatggtcaga aagttaggag aaacaagact aacacataaa
      901 atagatgtaa tgattatttc agcaacttct gagccaatag attctcattt acttaataca
      961 tttaaaagac gtatacctgt aacaattgaa ataccagaat tagttgcaag accattaaat
     1021 gagaggtttg acattataaa taatttcttc ttaatagaag ctcaaagaat gggagcaaac
     1081 atacatataa agtcagatgt attaaaagct cttatgcttt acgattgtat aggtaatgta
     1141 ggtcaactta gaagtgatat tcaagtagct tgtgctaggg gactattaaa ccaacttaca
     1201 aacaaactaa aagaagtaaa tataagcata tcagatgtac ctggatatgt aaaacaaggt
     1261 cttataaaaa tcagaaattg tagaggaaaa atagaaaatt atgtagatgg ggatttaatt
     1321 atagactcaa atcttcaagg tgagatagaa aagaaaaatg aagatatata tacttttcca
     1381 gatgaaatat acaaacttac tgaaagaaga catattgatt tattggaaca aggtttagat
     1441 tttgatgtta taaatagaat cattggagga gaattagaaa gttgcattca aaaatacata
     1501 aaacaagtta aaaaagtttc tattagtaac aatcttccag atataagtcc aattgttgga
     1561 aaatatatcg tagagctaac ttatgaagta atcaaaatag catcacaata cctaagttca
     1621 ctagaaccga atctacattt atgtttagca gttcacctaa aagcagcttt tgagagatta
     1681 aaagaaggaa aagtaatatc aaatgctcat ttgaaagaaa taataggaaa ttatccacta
     1741 gaatattctg tagcagaaaa ggtagctcaa tatttaaagg aaagatataa cataaactta
     1801 ccaaaagaag aaataggtat aatagcaata tattttaaaa tgagtagtag aagagatatg
     1861 tctgaacaaa aaaagatagg tgtacttctt atggctcacg gaagagtagc atcttctgta
     1921 tgtgaggtta caaataaact attaggaatt aatcacgcaa ggtatttaga tatgccattt
     1981 gagaagaaac cggaagaaat gattgaagaa gctactacaa taataaaaga tattgatgaa
     2041 ggtagaggtg tgattatatt agtagatatg ggttcacttt catattttgg agatattatt
     2101 tctggaaaga ctggaattaa tataaggaca atatctagat ttgatacatt gctttctata
     2161 gaggttatac gaagagcagt tttaccagat ttaacaatag acgagatagt tgatgaagtt
     2221 aaaggtatag agccacaagt aaaagaaaag gttagtaaat gtaataatac aaaagtagat
     2281 tttagaaaac ctataatttt aacattatgt attactggat tagggggagc ttgtaagata
     2341 aaaaaaatcc tagaggaaaa tattaaaagt ataactgaaa aggttgatat agaacctata
     2401 ggtatgattg aatatccaag tcttgattta gagataaaac aaataagcaa tgataaaaat
     2461 gtaatcgcca tagttggaac aattgaccct cagatagcaa atatcccttt ttttagtcta
     2521 gtagacataa atgataaagc aaagttaaat caaataaata acctaattga tacaaatcaa
     2581 attggtgaaa caaataaaaa atatgaatta tcagatttaa tacataaaga agatatattt
     2641 gtggatttac aggttaaatc aaaagaagaa gttctaaaat atatgagtaa aaaacttaga
     2701 gagaaaggtt atgtgacaaa aaatttctat aaaaaagtag tggaaagaga taacttattt
     2761 ccaactgaat tatcagatga aatagcaata cctcacacag atggaataga tgtaattagg
     2821 ccagctataa gtatagccat cttaaagaag cctattgtgt ggagtaaaag taaagtaaaa
     2881 ataatacttt tattagctgt agaccaaaag tgttttaatc cattaagcac tctactctca
     2941 tttatagaaa cagatgaatt taagaagata aaaaacatta aagatagaga ttttattagg
     3001 gagatgatat tagatggagt caatgaaaat aattagtagc aatttaattt ttaaaaatat
     3061 tgaagtgtct aacaatgaag atgcattaaa gtttttggga caacgtttgt ttgacgaaca
     3121 gtatgtaaaa gaaagctaca tacaagctgt tgtagcaaga gaaaaaaagt atgctacagg
     3181 gttaccaaca gaaatatacg gagtggcaat accacataca gatatagtac atgtaaatga
     3241 gccaggaata gcaattggaa tactgaataa acctgtaaaa tttataatga tgggtacaga
     3301 cgatactgaa attgatgtaa aagttgtatt tatgttagca gtgaaagagc cacaagaaca
     3361 attgcaacta ttagaaaaac ttatgactat atttcaagat aagaatatac taaataatat
     3421 tgttgactta agtgaagaaa gtgtgagtga tttattaaac tctaaattga aaaattaaac
     3481 atgtaaagta tattaaaaat gtaaaattaa acatgcaaag tatgttaaaa aatataaaaa
     3541 gattattaaa gatttctaac tatagatatc tttaaaataa aataatttca atgggggtga
     3601 ctaatgtggc taaaaagata ttagttgcat gtggaacagg agtatgtact tcaacaattg
     3661 ctataaacaa attaaagaaa gcacttcaag acattggtaa attggatatg gttaatatta
     3721 cacaatgtaa agtagtagaa gttgcatcaa aagcacctga ttatgattta ataatttgta
     3781 ctacacaagt atctagtagt ataaaaacac cagtgataaa tggattacct tttttaactg
     3841 gtgtcggaat ggataaatta atcaatgatg tattagaaga gttagaatta taattaaaaa
     3901 aattaaggaa catggtctct aaaatcataa gttttagaga cttaaaatta aataatggag
     3961 ggtctttata tggagatatt aaatttcatt gttggtctag gtgcacaagt aatgatgcca
     4021 ataataatat gtatttttgg tttgatatta ggtacaaaac taggaaaatc tttaagagct
     4081 ggtttaactg tgggagttgg gtttataggt cttaacacta ttatagcttt gctaacagat
     4141 aatttaggtc cagctacaca acaaatggtt aagaatttag ggctaagttt atcaataata
     4201 gatgtaggat ggccagcagc atcagctata gcttttgcat caacagtagg ggctttaatt
     4261 attccaatag gtcttgtggt aaatatagtt atgctaatta ccaatactac tcaaactgtt
     4321 gatgtagaca tatgggatta ctggcatttt gcttttacag gagctttagt ggcaggggct
     4381 actcaaagtg taatgtgggg agtatttgca gcagtagcaa atatggtaat tgtattggta
     4441 atggcagatt taactgctcc aggtatagag gaatatttag gtatgccagg tatatctctt
     4501 cctcatggat ttacacaggc atttgttcca attgctatag tagtaaataa attattagac
     4561 ttaataccag gcataaataa gattgagata aatgctgata cattgcagaa aaaatttgga
     4621 ttatttggag aacctttaat aatggggtct gtaataggtg taataattgg tattgctgcg
     4681 aaatatgata taaaaggaat attacaatta ggtgtaacta tgggagctgt attaatatta
     4741 attccaaaga tggcagcatt attaatggaa ggattattac cagtatcaga agctgcacag
     4801 gaatttattg agaagagatt taaaaacaga ggcaagattt atataggctt agattcagca
     4861 gtaggaatag gtcatcctgt tacattatca gttgcactag tgttggttcc tttgactata
     4921 cttatagcag ctatattgcc aggaaataaa gttttaccat ttgcagattt agcagtaatt
     4981 ccatttgcac ttgtattaat cgtaccaatt acaaagggaa atgtgtttag aacattaata
     5041 ataggaataa ttataattac ttctggatta ttaatcgcaa ctaacttagc tccattattt
     5101 acccaaatgg ctttaaatgc tagttttaaa atgcctgaag gagcaactat gatttcaagt
     5161 atttgtgatg gtgcgaatcc attaagttgg gtatttgtaa aagttatgaa ctataaagtt
     5221 attggaggag tagtttttgg agttatagct ttaggaatgg ctatttacaa taggaataga
     5281 ataataaatg aaaataaaaa gctatctata gaagaataag gttaaaacaa ttttattaag
     5341 gagtgtatag gttatgaaaa aatataaata tgattctaaa agatacttac tcagtgttac
     5401 aataccagga ttttttatga ttttaatatt gatttatgca ctgtttaata actatacaaa
     5461 ttttaatttg aatatatata gtttgttaat aattgtctgt acttatggta tattcaatac
     5521 ttttatatca tcaagtaatc ctaaaaaaat tattattgat agcaaatgta tacattttac
     5581 ctcatttatg actacacata agtatgaaat taaagatata gaaaagataa gaataagaga
     5641 attttataat aaaaaaattt atctaaaaat taatgatgga aaacttttca aaggtaggta
     5701 ttggataaga acaaatatgt ttaataatag tatagcatta tatagctatt tcattgagtt
     5761 ggaagagtgc ttatatccag attcactaaa atttagaaat aaaaaatttg agaataagaa
     5821 catataaaaa taaaaaattg aattgaagcc tagcttaatc ttttttgata agatataagt
     5881 ttgaacttta tactaaattt ttagaatttt caatcaagac aataacataa tagctaagca
     5941 attagaacac aactaagatg tataaggcat gtatatgatg aatgggagga tttaattaat
     6001 gttattagag aaattaagaa aagaagtatt acaagcatct ttagaccttt taaactataa
     6061 tttagtgaca ttaacaggag gaaatgtaag tgggagagat gaacaaactg gctatatagc
     6121 aatcacccca agtggaatgg attatagaaa tcttacacca agtgatattg ttatagtaga
     6181 tgtagatgga aatattatag atggaaaatg gaaagcatca gtggatttat cagaccactt
     6241 gtatatatat aagcatagag aagacataaa tagtataatt catacacatt ctacatactc
     6301 aagttgtttt gctattttaa atgaacctat agaatgtgca agtacaactc ttgcaaatga
     6361 agtagggggt tcagtaccag ttgcaaaatt ttcgccacca acatctaaaa agatgggaaa
     6421 atgtgttatt gaagcaattg gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat
     6481 tgcagttggt ccatctgtag gacatgctct tacagccgct gtaatgttag aagattcagc
     6541 aaaagtatat tacttagcaa aatcaattgg cacaccagtg ctactgcctg atgaagaaat
     6601 tcaaagagcg agagatgtat tttttaatgt ctatggtcag gataaataaa ttagataaaa
     6661 ttttaaaagt aatatgaaaa tatagatatt caatgatatt aaggagattt ataatgatat
     6721 aataagttta atagttaaga agtaaaatat gaaatgtaat agtatataaa ttcatttaag
     6781 actaaaaaga ataagataaa ataaaaagga gtctgtattt ctatgtatta cttattaaac
     6841 aaaagcacaa atccttactt taatttggca ttagaagaat atctattttt aaatgataaa
     6901 tataatgatg atattataat catatggaga aatgaagaat ccatttttat aggtaaaaat
     6961 caaaatccat atcaggaagt atatcatgat gttattgaaa agggagaaat tcctatttta
     7021 agaagaatat ctggaggagg aactgtttat catgacttag gtaatatcaa tatgtcattc
     7081 atacaaaaag ataggcaact tcatgagata gattttttgg agcatactaa gtttatgcaa
     7141 aagatgcttt caaccttagg actagatgta tctataactg aaagaaaaga tttattttta
     7201 aatgggaaaa aaatttcagg ctctgcacag tctataaaaa gaaaaaattc tctttatcat
     7261 ggaactcttc tttacgattc agatttaaat aaattaacta aatatttaaa ttcaaataaa
     7321 gcaactgaat caaatgcaac taaatctgtt tcaagtaaag ttacaaatat taagttttta
     7381 ctcgaaaaag atataaatta ctttttagat tattgtgttg aatatttaaa atctaatata
     7441 aagaatataa aagaacttga attggatgaa gaagatatta aaagtgtata caatatagga
     7501 gaagataaat ataaaaagat agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg
     7561 ctaccattta ataatgaaag taaaataaat atacatgtta acagatggaa agtatcaaaa
     7621 ttttttattt cttatgatga taatgtaatt aatatggaca agttgttaga tttgaatttc
     7681 tttaaagaag aaataaaaga gagtatttta gaaaattatc cacattacat agatttaatc
     7741 aatttaatat tttaatataa aacctctaat ttgataaaat tgtcttatat caaactagag
     7801 gtttttaata ttcttgcata ttaacaacca ttacaaaatt gtaaaattaa aaacaacact
     7861 attgaaaaat caatatatat tgtaataaat atatgtgttt ttcattagca aatagacatt
     7921 aactatttat acaactgaaa atttaatata taattaatca gatggttaaa ataaaaatat
     7981 taaaagtttt aataaatgat ggttaaaaat aaaatacgag gtgataccat gtatagtgtt
     8041 ataattgttg cagcgggaag cggaaggaga atgaacttag atataaacaa acagttcata
     8101 aaattaagag aaaaagaaat tattgctcat acaattcaag ttttttatga aaatataaat
     8161 atagatgaaa tagtggtatg tataaaaaaa gaagaagaag atttttttaa agaaaatata
     8221 ataaataagt ataactttaa aaatataaaa atagcatatg gcggaaaaga aagacaggac
     8281 tcaatatata atggtttaaa aaaattagat aaaaattgtg atatagtact tatccatgat
     8341 ggagcaagac catttgtcga ccatagaata atcaatgaat ctataaaggt agctaaagaa
     8401 aaaaaggctg tggttgtagg tgttccagta agtgatacta taaagatagt gtcagatgga
     8461 acagtacaag aaactccaga aagaaatttg ctgtgggcag cacaaacccc tcaaaccttt
     8521 gaatataatc tcataataga tgcttatgaa caagcatata agaacaatta ttatggtact
     8581 gatgattcaa tgttggtaga aaatataggt caaagtgtca ctatggtaat gggttcttat
     8641 gaaaacataa aaataactag tccagaagat ttaaatatag cagaacaaat attaaatatg
     8701 gaaaaaagag atgaagttaa cagtagaagg agaattatat gagaatagga ttaggatatg
     8761 atgtacacaa attaacagag gatagaaaac ttataatcgg tggtgtagag ataccacacg
     8821 ataaaggttt gttgggtcat tctgatgctg atgtattgat acatgcaata atggattcta
     8881 ttttaggtgc tctagcatta ggagatatag ggaagcattt cccagataca gatgaagaat
     8941 ataagggagc agatagtatg aaattattag aacatgtgta taatctaata acaagtaaag
     9001 gatataaaat tggtaatata gacagtacta taattgctca aagccctaag atggctccat
     9061 atattgagag tatgagaagt aatatatcaa aagttttaaa cacagacatt gataatataa
     9121 acataaaggc aacaacagaa gaaggtcttg gttttacagg agcaaaacag ggaatagctt
     9181 cacaaagtat ttgtttatta ttgttgacaa gtcagaataa ttaatatatt attagtaata
     9241 agtttaatgg atatcaagta ataggcctat atagaatatc tataatgtat gaatttaagt
     9301 gaaattaata taaatataaa attagctatg ataaagaaat agtaaaatga tgaaatcttc
     9361 acagagagaa aacggtggtg agagttttca agaggaagtc tttgaacctg tcttttagct
     9421 taaaggtata aaaatcttta cggtaggaac cgttatattc ccaaaagaga gctattcata
     9481 tagctaacta gagtggtacc gcggaaatta aacctttcgt ctctatactt gtatagagat
     9541 gagaggtttt ttatattttc aggaagttaa tattcaggag gaatctatta atgaaaatgt
     9601 caaaaatgtt tatgccgaca ttaaaagaaa tcccagcaga tgctgagatt acaagccatc
     9661 aattaatggt taggtcaggc atgataaaaa aaatgacttc aggtgtttat aaccaattac
     9721 caatggggtt aagagtgttc aaaaaaattg aacaaataat tagagaagaa ttaaataaaa
     9781 aagattgtca agaaatactt tgtgcagcct tacttccttc tgaattatgg aaagagtctg
     9841 gaaggtggac tgctatgggt gaagaaatgt ttagactaaa agacagaaca gaaagagagt
     9901 actgtcttgg gccaacacat gaagaagcat tcactgatat aataagacaa gaaattactt
     9961 catataaaca attgccactg aacttgtacc aaatacaagt aaaatataga gacgagagaa
    10021 gaccaagatt tggtgttatg agaactaaga cttttacaat gaaggatgcc tatagttttg
    10081 atgtagatga taaaggttta gataagtcat atcaagatat gtttgatgca tatgtaagta
    10141 tatttaatag atgtggattg gagaatagcc ctgtacaggc agattcagga gctataggag
    10201 gttctacatc agccgaattt atggttaagt cagaagttgg tgaagatgaa gttgtatttt
    10261 gtagtggatg tgattatgca gccaatgtag aaagagcaga atcttgtaat ctagcatcac
    10321 aaaaagaaga aatgaaagaa ttagaagagg ttcatacacc aggagcagct acaataaaag
    10381 agttagaaga atttttaaaa acttctccag ataagtttgc taaaacttta gtatatgagg
    10441 cagatggaaa aacagttgta gtcgttgtaa gaggagatag agaagttaat gaaatcaaag
    10501 tatctaatgc cataggttct gttatagaat ttgctcttgc tacagatgat gtagtgagaa
    10561 aagtaactaa tgcagaagta ggatttgcag gtccaatagg tataaatgct gactatgtat
    10621 ttatagataa agaaattgtt gaacaaagaa acatagttgt tggggctaat aaaactgagt
    10681 accatataaa aaatgctaac tatggaagag attttgaagg aatagttgga gactttagaa
    10741 atgttcaaga aggcgacaag tgtatagtat gtggtaagcc acttgaaata gctagaggtg
    10801 ttgaagtagg acatatattt aaactaggaa ctaagtactc agaatctatg aatgcaaact
    10861 tcatagacaa agatggaaaa tcaaagccta tagtaatggg atgttatgga ataggtgttg
    10921 aaagaacagc agcagctata attgaacaac ataatgatga gaaaggtata atatggcctt
    10981 tgtcagtagc tccataccat gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa
    11041 tatcaatagc tgaaaatata tataatgatt tgcaagctat gggtgtagaa gttttattag
    11101 atgacagaga tgaaagaata ggtgtcaaat tcaatgattc agaattaata ggaataccta
    11161 tgaggattac agtaggtaaa aatattaatg aaggtaaagt agaatttaaa cttagacata
    11221 aagaagataa agagataatt gacatagaag aaataaatga aaaagtaaag gcagagttta
    11281 tcaggaataa tgttaggtta ggataataac ttaaataaga tattgtatag ataatcaaag
    11341 ttgtacttta tagacaccat tttatctata tataaggata taaaatggtg tttttttata
    11401 aaacaaataa atgaggaggt aattaaaatg gcaaaaaatg aaaaacaatt tgttgaagaa
    11461 ataacaaaga tggaagatga ttttccacaa tggtacacag atgtaataac taagactgac
    11521 ttagttgatt atgccccagt aaaaggtttt atggttgtaa agccatatgg atatgcatta
    11581 tgggaaaaga tgcaagaatt tatggataaa aaatttaaag aaactggaca taaaaactgt
    11641 tattttccat tattaatacc agagagttta ttaaacaaag aagcagaaca cgttgaaggt
    11701 tttgctccag aagttgcatg ggtaacacat ggaggaaata aaaaattaga agaaagatta
    11761 tgtgtaagac ctacttcaga gactataata tgtacaatgt atgcgaaatg gttaaaatca
    11821 tatagagaat taccatatct ttataatcaa tggtgctcag ttgttagatg ggaaaaatca
    11881 actagacctt tcttaagaac atctgagttt ttatggcaag aaggtcatac gttacatgaa
    11941 acagcagaag aagcacaaga agaaacaata caacaattag aagtatataa agcgctatgt
    12001 gaagaattac ttgcaatgcc agttgtagct ggtcaaaaaa gtgaaagtga gaaatttgca
    12061 ggtggtgaaa gaacatatac tatagaagct atgatgcatg atggtaaagc attacaatca
    12121 ggtacaagtc acttcctagg tcaacacttt actaaggctt ttgatattac ttttgcagat
    12181 agggaaggaa atttagctaa tccataccat acatcttggg gagcatctac aagacttata
    12241 ggtggactta taatgactca tagtgataac agaggtttag tattaccacc aagagtagct
    12301 ccaattcaag ttgttatagt tcctatagct gctaaaaaag gaaatgtaat ggagactgtt
    12361 gataaaatat atgctgattt aaaagctaag ggagtagctg tagaagttga tgatagagat
    12421 aactatacaa ctggatggaa atttaatgag tgggaaatga aaggtgttcc agtaagagtt
    12481 gaaattggac caaaagatat agaaaataat caagctatgg tatttagaag agatacttta
    12541 gaaaaggatt ctatgccttt agaaggatta gctgatgcta tatgtgattt atttgatgtt
    12601 atacacaatg atatgtttga aaaagcaaga aaacatagag aagacaatac atctatagtt
    12661 gaaaatatgg atgagtttag aaaagcttta gaagaaaagc caggattcat aaagactatg
    12721 tggtgtggag atgcagaatg tgaagctaag attaaggaag aaacaggagc aactataaga
    12781 tgcttacctt ttgaacaaga aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca
    12841 gatagtatgg tagtaatggc aaaagcttac taaaatacat ttaaataaaa actggggtag
    12901 aaagtattta atactttatc ccagtttttt gttttttatg agtaataaaa tgtatttgta
    12961 actatgtcat aagataagtc atagatactt atattaaaaa gaatataata gagaactatt
    13021 tttaataatt acatattaat gaaagcaaaa tattttgtta tatagatttt caataaagca
    13081 aacaataaat ataaatgaat attaataagg agtgctttta atttttagaa aataaatgac
    13141 ttaaaagaaa gatttatgaa attaaaacta ggattttctg atataataaa catgtgtatt
    13201 tttaaataaa aaggatatta ttattagatt tgattaaaaa taaacttgca aaacaatgta
    13261 ctagtttaat attatagtta tattgaataa aatatcagga ggaagtaaaa atgaatgtaa
    13321 gagtaaggtt tgcaccaagt ccaacaggat ttgtacatat aggtagtttg agaactgctt
    13381 tatataacta tttatttgca aaaaaaatgg gcggagaata tatattaaga gtggaagata
    13441 cagaccaaag tagattagta gaaggtgcta tagagaatat gcttaatgct atgaaatggg
    13501 ctggggtcaa ccatagtgaa ggtgttatat tagatgatag tggaaaagta gtacaaaaag
    13561 gagaatatgg tccatatatt caatctcaaa gattagatat ataccaagaa tatataaagc
    13621 aacttttaga tagtggaaaa gcttattact gtttctgtac aaaagaaaga ctggatgaag
    13681 taagagatgc tcaaagagca gcaggagaaa cagctaaata tgatggtcat tgtaagaatc
    13741 tttctaaaga agaagtagaa gcgaatataa aagcaggaat accatatgtt ataagattaa
    13801 gattgccaga gaatcataca attaagttta cagacttagt tagaggagat atggaattta
    13861 atactaatga cttagatgac caagtattaa tgaaaacaga tggatttcca acttatcatt
    13921 ttgcagtagt tgttgatgac tatttgatga aaattacaca tgttataaga ggagaagaat
    13981 gggtatcatc tactccaaaa catgtttatt tatatgaagc ttttggatgg gaagcacctg
    14041 tatttgtgca tttaccaaat atacttaata aagaaaagaa aaaattaagt aaaagacaag
    14101 gtgatgtagc tgttgaagac tttaagaaaa aaggatattt accagaggga ttagtaaact
    14161 atgtagcatt agttggttgg tctcctgaag ataatcaaga attgtttaca atggaggaat
    14221 tagaaaaagc cttctctgta gaaagagtat ctaaaagtgg tggagtattt gatactgaaa
    14281 aattaaattg ggttaatcag cactatataa aagatggtga tgatgcttat ctaacagact
    14341 tagcaatacc atttttaata gaagatggat ttataacaga agaagaagct actaataaat
    14401 atgatttctt aaagagtatg atatcagtac ttaaagagaa attacaatat gtaaaagaag
    14461 ttactgaaca tgcaagtata ttttttggag ataaaataga ggtagaaact gaagagggaa
    14521 gagagttctt aagattagaa catataccta ctttaataga tgctttgaga gaaaaaatag
    14581 aaaaaacaga ggtattaaat gctgagtttg ttcaagctat gcttaaagaa atacaaaaag
    14641 agtacaaaat aaaagggaaa aatttattta tgggctcaag aataattttg acagggcaaa
    14701 tgcatggtcc agatttacca aaagtaatgg aagttttagg aaaagaaact tgcttaaata
    14761 gaatagctta tgtaaaaaat aatatattat agtcataaaa gtccgctaat ttagaagcgg
    14821 actttttttg tgttagtatt tggtgaaaat atctgcttaa gaaaatgaag gtatttaagg
    14881 taataaattt aatatataaa aagcaaagca aaaatataaa taaaatatga ataataaact
    14941 aataaaatat taagcataga agaagggaat ctattatttt agtttgattt aattattaag
    15001 ggggtaatta ctatgtatag taatattgat gatgttaaaa aggaattaaa ggagctatgc
    15061 ttagaatatg ttacaatttt agaaaaatta aaagatgaaa aaatgattac agaagaaact
    15121 tttgaaaagt gctcttctca aaaaaaaata tttttagaag aacaatagag gatggtaata
    15181 gtattattat tatgtagttt gaaagatagt acgaaaaatg atagtaatac tattatttat
    15241 tgcaattttc aactataata taatatataa tgttaagaag ataatttata aaaatacagt
    15301 gaggtgatga gtgtgaaagt ttataataca ttgactagaa caaaagaaga atttgttcca
    15361 ttagaagaag gaaaggtaaa aatgtatgtg tgtggtccaa cagtttataa ctatatacac
    15421 ataggtaatg caagaccatt tataatattt gatactctaa gaaggtattt agagtataga
    15481 ggatatgatg taacttatgt acaaaacttt acagatgttg atgataagat tataaataga
    15541 agtcatgagg agggaatatc tcctgaagaa gttgcggcta agtatataaa agaatatttt
    15601 gtagactgtg atgggctagg aataaaaaga gctactgttc atcctcaggt tacagataat
    15661 atacaacaga ttatagagtt tataaaagag ttggaagata agggatatgc atatgcagta
    15721 aatggtgatg tatattttga tacaaataag tttgaaggtt atggaaaact ttcaggtcaa
    15781 aaacaagaag atttagaggc aggggctaga attgaggtta atgaccaaaa aagacatcct
    15841 atggactttg ttctttggaa ggcaaaaaaa gaaggtgagc caggatggga tagcccttgg
    15901 ggagaaggta gaccaggatg gcatatagaa tgttctgtta tgtctaagag atatttaggt
    15961 gaaacaattg atatacatgc aggtgggcaa gatttaacat tcccacatca tgaaaatgaa
    16021 atagctcaaa gtgaagctag aagtggaaaa acattctcaa aatactggat gcataatgga
    16081 tatataaata taaatgatga aaagatgagt aaatcaaaag gtaatttctt tacagtaaga
    16141 gatatatcaa aattatatga tttagagata gtaagatttt tcatgttatc agcacattat
    16201 agaaatccag taaactttag tgatgagatg ttaaatcaag ctaaagcagg tcttgaaaga
    16261 ctatataata ctaaagaaaa attagaattt acgctaagta atttagtaga atctccttta
    16321 acagaaaaag aagtagagct tgttaaagaa ttagatgact ttagacaaaa attcatagat
    16381 gctatggatg atgatgttaa tacagcagac gcagtaagtg taatatttga attagctaag
    16441 ttaataaatt ctaatgtaga tgaaaattct tctttggagt ttgctaaaaa atgcttagat
    16501 gaatttaatg aacttacagg agtattaaat attgtaaata agaaaaagga tactgtatta
    16561 gataaagaca ttgaagaatt gatacaaaaa agaactgatg caaagaaaaa taaagagttt
    16621 caattagctg atgatattag acaacaatta ctagatatgg gaattgtatt agaagacaca
    16681 agacaaggtg ttaagtggaa aaggatataa aaatggaaaa aactgaatta gttactatgt
    16741 caccattagt tttagcatac ttaggtgata cagtgtatga gacatatata agagaatatt
    16801 taattagaca gaatacacaa agaaaagtta atgatttgca taaactagct ataaagtatg
    16861 taaaagctaa agctcaggca actataattc atgaagttga aattgagtta actgaagaag
    16921 agagtaaaat ttataaaagg ggaagaaatc aaaagtcaaa tacttctcct aagaatgcag
    16981 atattataga ctacaaacat gcaactgggt ttgaggcatt agttggatat ttgtatttaa
    17041 ataatgaaat agagagactt caatatatta tcaataaagg aatcaaaatt attgaaagag
    17101 atatgtaaat attaaagagt gttgtcttaa taataagtaa taaggcaaca ctttttttat
    17161 aactaatgta taataaatat atagaaatta gtaatgagaa aggataaaaa gttatgaaag
    17221 taaaattaat atcacataca cctgaaccag aaaaagtaat agctatggca gctaagctat
    17281 gttattcacc agttgggaca gacgaaatag aaaaagactt aacagatgaa agtatagaga
    17341 aatttttaaa tatgttatta agtataggtc atgggtcaat attagaacat gcttcattta
    17401 cattttcaat agaaggtatt tcaagggcat gttcacatca aattgttaga catcgtatag
    17461 ctagtttttc acaacaaagt caaagatatg ttaaattaga acaatttgag tacataattc
    17521 ctccagaaat agaaaaaata gaaaaggcaa aagagttatt tatagactcc atgaaaaaag
    17581 accaagagaa ttatgataag ttagttgaaa tattgtttga aaatcattat aatgacctaa
    17641 taaaaaacgg taaaaatgag aaaacagcta aaagacaagc tgagaagaaa gcaatagaag
    17701 atgctagata cgtatttcca aatgcatgtg aaacaaagat ggtatttact ataaatgcta
    17761 gaagtttgtt taatttcttt gaacatagat gctgtgaaag agcacagtgg gaaataagaa
    17821 atttagctgt ggagatgtta agagaggtta aaaaggttgc tcctatatta tttaaaaaga
    17881 ctggtccaag ttgtgtaaat ggaagttgtc cagaaggaac tatgacttgt ggagatatag
    17941 ttcaagttag agaaaaattt aaggccttgt aggaggtgaa taatttggcg agtatagaag
    18001 gaagaaatcc ggtaatagaa gcaataaaaa gcgacagaga aatagataaa atattaattg
    18061 caaattcagc taaagaaggt tcaattaaga aaataatagg aatggctaaa gataagaata
    18121 taattattca gtatgttgat aaacataagt tagatgaagt aagtacaagt cattcacatc
    18181 aaggtgtaat agcttatgca agtgagtata agtattatga attagatgag ctaatagatt
    18241 tagcaaagaa taaagatgaa gacccatttt tcattatatt agatgagata acagacccac
    18301 ataatttggg ttcaataata aggacagcag atgcagtagg agcacatgga gttatcattc
    18361 caaaaagaag gtctgtacac ataactcctg tagttgccaa agcatctgct ggagcagtag
    18421 aatatatgcc agtttgcaag gtaaccaata tagttaatac aataaaaaga ctcaaagaag
    18481 aagggctgtg gattgctgca gctgatatgg atggagaaac tttttataaa caaaatctta
    18541 ctggtcctct tggtgtagta attggaagtg aaggttttgg aatatctaga ttggttaaac
    18601 aaaactgtga ctttatagta aaaatgccaa tgataggtaa tgttacatct ttaaatgcat
    18661 ctgtagcagg tggaatactt ttatatgaga tattcagaca aagactggat aaaagtaaat
    18721 aggcatgaaa aaaaatataa atcattactt aataatagat ggctacaata ttataaatgc
    18781 gtgggacaat ttgaaagaac tggctaaaga ggacttagaa gattctcgag aaaagcttat
    18841 tgatgacatt atagaatttt ctgagtttat gggttataag actataatag tttttgatgc
    18901 ttataatgtt aaaaattctc gagaaaaagt tgaaaaaaga aaacacataa caatagttta
    18961 tacaagagaa caccaaacag cagatagtta tatagagaaa tttataactt ctttatcaaa
    19021 gtatgatgat gtaaaagtag ccactaatga ctatgcagaa cagcaaatga ttttaggaaa
    19081 aggtgctact agaatgtctg caagagaatt aaagttagag ttagatcgtt ccaagaataa
    19141 aatgaaggaa aaaaatatcg gtttgaggaa aaaaattcag cgaaattggt tggaggaaag
    19201 gttagataaa gaaacattgt cgaaacttga gaacattcgt agaaagcgtt gaaattacta
    19261 ggttctttag actataatat acctataaat ttgttttata tatgggggag atttacatgt
    19321 tggtagcaaa agaaaaaagt tatgagttag tggacaattg tcagcaggat gagtataaca
    19381 ttgtattaag agcaagtgaa ggggataaaa tagcactaga gtacattatt actaaataca
    19441 gaaattttgt aaaggcaaaa gcaaagtcat attttttaat tggagcagat aaagaagata
    19501 taatacaaga aggaatgata ggcctttata aggcagttag agattttgat ggaagtaaga
    19561 ccaattcatt taaatgtttt gcggagatat gtataacaag acaaataata actgctataa
    19621 aaactgcaac aagacaaaaa catatacctt taaattcata tgtttctttg aataaaccaa
    19681 tatatgatga agagtcagac agaacacttt tagatattat agctacaagt atagtaacag
    19741 atccagaaga gcttataatt agtaaagaag agttaaaaaa tatagaatca aaaatgaacg
    19801 aattgttaag tgacttggaa ttagaagtac tagaattata tttaaatgga aagtcatacc
    19861 agtttatagc ggacaagctt aaaagagatg taaagtcaat agataacgct ttacaaaggg
    19921 ttaagagaaa attagaaaaa catcttgaaa acagaaatga ttaatagtaa aatacttacc
    19981 ataagcttat tgtgagcaaa attaatttat atcgaaagat aagggaggaa attcaaaatg
    20041 gctaaagcta aatacgaaag aacaaaacct catgttaata tagggacaat aggacacgta
    20101 gaccacggta aaactacatt aacagcagca ataacaaaaa cattatatga cagatatcaa
    20161 ttaggagaag cagtagattt cgcaaacata gataaagctc cagaagaaag agaaagagga
    20221 atcacaatat caacagcaca cgttgagtat gaaacaccaa atagacacta tgcacacgtt
    20281 gactgcccag gacatgctga ctacgttaag aacatgataa caggagcagc acaaatggac
    20341 ggagcaatat tagtttgttc agcaacagat ggaccaatgc cacaaacaag agagcatata
    20401 ctattatcaa gacaagttgg agtaccatat atagtagtat tcttaaacaa atgtgacatg
    20461 gtagatgatg aagagttatt agagttagta gagatggaag taagagattt attaacagaa
    20521 tatgatttcc caggagatga cactccaata gtaagaggtt cagcattaat
//
LOCUS       DAELTA010000065        20551 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.065, whole genome shotgun sequence.
ACCESSION   DAELTA010000065 DAELTA010000000
VERSION     DAELTA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.065"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            368..1348
                     /locus_tag="KPG13_001958"
     CDS             368..1348
                     /locus_tag="KPG13_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0985691.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1447..2409)
                     /locus_tag="KPG13_001959"
     CDS             complement(1447..2409)
                     /locus_tag="KPG13_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0985692.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2725..3675)
                     /gene="hpdA"
                     /locus_tag="KPG13_001960"
     CDS             complement(2725..3675)
                     /gene="hpdA"
                     /locus_tag="KPG13_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG0985693.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3668..3925)
                     /gene="hpdC"
                     /locus_tag="KPG13_001961"
     CDS             complement(3668..3925)
                     /gene="hpdC"
                     /locus_tag="KPG13_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG0985694.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3929..6637)
                     /gene="hpdB"
                     /locus_tag="KPG13_001962"
     CDS             complement(3929..6637)
                     /gene="hpdB"
                     /locus_tag="KPG13_001962"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG0985695.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6934..7950)
                     /gene="tsaD"
                     /locus_tag="KPG13_001963"
     CDS             complement(6934..7950)
                     /gene="tsaD"
                     /locus_tag="KPG13_001963"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG0985696.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7956..8432)
                     /gene="rimI"
                     /locus_tag="KPG13_001964"
     CDS             complement(7956..8432)
                     /gene="rimI"
                     /locus_tag="KPG13_001964"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG0985697.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8422..9138)
                     /gene="tsaB"
                     /locus_tag="KPG13_001965"
     CDS             complement(8422..9138)
                     /gene="tsaB"
                     /locus_tag="KPG13_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG0985698.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(9138..9590)
                     /gene="tsaE"
                     /locus_tag="KPG13_001966"
     CDS             complement(9138..9590)
                     /gene="tsaE"
                     /locus_tag="KPG13_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG0985699.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9729..10769)
                     /locus_tag="KPG13_001967"
     CDS             complement(9729..10769)
                     /locus_tag="KPG13_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0985700.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(11098..11703)
                     /locus_tag="KPG13_001968"
     CDS             complement(11098..11703)
                     /locus_tag="KPG13_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0985701.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(11828..11945)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12005..13171)
                     /locus_tag="KPG13_001969"
     CDS             complement(12005..13171)
                     /locus_tag="KPG13_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0985702.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(13319..15460)
                     /locus_tag="KPG13_001970"
     CDS             complement(13319..15460)
                     /locus_tag="KPG13_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG0985703.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15604..16711)
                     /gene="prfB"
                     /locus_tag="KPG13_001971"
     CDS             complement(join(15604..16635,16637..16711))
                     /gene="prfB"
                     /locus_tag="KPG13_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG0985704.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKKTLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(16726..19401)
                     /gene="secA"
                     /locus_tag="KPG13_001972"
     CDS             complement(16726..19401)
                     /gene="secA"
                     /locus_tag="KPG13_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0985705.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19533..20084)
                     /gene="raiA"
                     /locus_tag="KPG13_001973"
     CDS             complement(19533..20084)
                     /gene="raiA"
                     /locus_tag="KPG13_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0985706.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
BASE COUNT         6283 a         3784 c         2153 g         8331 t
ORIGIN      
        1 aatcaaagag aataataaat gtaatattaa aactcaataa taaatttata ttcataagtt
       61 taatttcttc attaatttta tcaactatgc ctctactgcc tattaattta atgcaataaa
      121 taataaatct tttacaaaaa gataataaaa atatagaaaa atatttttta ttgtcctttt
      181 ggttatggta aaccacattc tgcctgcaat ataactaaat atatgggtag atagtaaaat
      241 taattttatt atttatatat aattttataa gttacttttc aataatattg actgtcatta
      301 atttataata ttcaataaaa tattttaatt tagttaagaa tctatattgt aaaggagtat
      361 taaattaatg aacttaaaat taatatggat aaatattcta aacccagtat ttattaaaaa
      421 tgaaataaaa agaaaaaaaa tacaaattat tatctttttc tttataaact ttggtttaac
      481 ttattcatta ggtttactgt tatattataa taaatttatt gatccagaaa actttgcatc
      541 attcatgatg atattacctc tatcttcagt tgccattgca aagttttata ctgaaggatt
      601 aaataatgat atgtatgaat tttattcact aatcatatta ttttttctta tttattcatt
      661 attaattgtt cttaagttat taaatctaat aaataataat gaatttataa ttataaacct
      721 aatattaact tttataagta gcttatcaat tataatatat tcatcaaata aaaaaaattt
      781 atatcctgta aaaaatatta aactaggttc tttattaatt atttacttta tgatttctgt
      841 aattgttcca acatcattta tactaatact acaaggggct caacctaatt atagtggtat
      901 cataaaattt atatttactc caataacatc attaactact atttatattt tttttagtga
      961 agaatatgcc tggagaggtt ttttacagaa tatttttttt gataaatttg gtaaaaagtt
     1021 aggagttata atattaggta tgtgttggag cttgtggcac ttaccactaa tatttacatt
     1081 atatacacct gaagcgccaa tattaggaat aatattacgt tcaatacata tagttggaat
     1141 ttcaatattt ttaggatatc tttatataaa aactaagaat atctggtttt gtgctataat
     1201 tcatgtttta aataatagtg cttttcttat cacaaattct aaagaatcaa ctatcactta
     1261 ccatgatata ctaatagtat ctatggttgt tttaattttt tatgttccat ttctatttac
     1321 aaaagaatat aaaaataact tggagtaaat agaatatcca agttattttt atatttttgc
     1381 aaatttccaa caatcctaca gaatacttta agcttagaag atattattaa aatttattat
     1441 aatcaactaa ttcttgatat tggtcacatt tactttatca ctaacaaaat catacttaga
     1501 atctctatag taataccata caaatataaa gctaagtaga ttaacaaaag tagtcaaaat
     1561 tccaccttca ataccaaaca ttcctccatt taatagatta ccatgagtga tttgtgtagt
     1621 tataatccct ggaacttgaa gtccacttac aggcatacca tatataatcc cctgaaatac
     1681 attccatgca aaatgataac ctatacacat ccatatattc gacgatttta taaacatata
     1741 tgaaaaaaga atcccaataa ttataatatt aattacagac aaaaaagtaa catcaggatt
     1801 ccatatatga atcaacccaa aaataacaga tggaacaatc attataaaat aaatattttt
     1861 acatcttcgt aaaactgcca taaaaaaacc acgatttaaa atttcttctg caaaagcaac
     1921 taaaataaag accagtatcc aagaaactgt taaaggtgaa atttttggta cccatgaagt
     1981 aacatgacca ccaccaattg ttattgttat aataaagatt actgtacaat taaatattcc
     2041 taataacatt cctacatatc cttctttttt cttaattcta taagaatgta atcccatttc
     2101 actaatagga tttttcataa taaatctcca tacaattata ggaataataa tcataagtga
     2161 atctgttaga atttgaaata caatctgtaa cccaacatca tttaaccaat tagcatattc
     2221 tgaaaaatag tttgttttta aattaatgtc acctgtagca accaggattt ttcttattat
     2281 ttccacaagt atcatgctta aataatactg aagtatgtaa aaggtagcca taacagctat
     2341 aataatccac cctgaacgta cttttttgtc tttattctta ataatcaagt tgctccatga
     2401 tttgttcata tagatgtctt cctcctaact attattttta gttgtttcta aaatatattc
     2461 tattaaattt tttcatagta gtattttacc acaaaacttc tgcttcaatg aagtaagcgt
     2521 aatatattct ctaactgtaa tttaaactat aaaaaatata taaagtatat ttaaagcaat
     2581 atttatggac ataaagcaat ataaacaata tataaaacat ataaaacttt acttaattat
     2641 ttctacataa aaaccacata aaaaaggatg taactctagc ttttagtgta acatcctttt
     2701 tttaattaaa acttgtattt aattttagaa agctgtctca tgaccaacat aacaagctat
     2761 accattatcc aaaaatatat cttgtaattc ttcaagatga ccttcatcaa catcaccctt
     2821 atcagaatac tcatattcct tgcctaattg aatccatttt gattctccta atctgtgaaa
     2881 tggtaataga ttaatttcta ccaaattatt tttgtgcata aaagatataa tatcacttat
     2941 attttcaaca ctatcattaa acccagagat aactggtact ctaagtacta atcttccatt
     3001 ccaatcagaa tttgcaaggt ttgatatatt ttctaatatt aaatcattat acacacctgt
     3061 ttgttctttg tgtttttctc tatccatatg ctttatatca ataaatgcaa aatcaatatc
     3121 gttaaatatt ttattaaaaa cttcatttga tacacatgca ctcgtttcaa tggcagtgtg
     3181 tatatttacc tcatgacact ttaataatac ttcatgtaaa aattcatgct gaagtagtgg
     3241 ctcacctcca ctaaatgtta ctcctccatt acttctccaa ttgttagaat cacgtttaat
     3301 tacctgcaca agttcatcta cagtgtaagg ttttgcacat aatttaaacg cattataata
     3361 acatgagttg acgcattcaa aactttcaca gtccttacag atgttccaat ctataactgg
     3421 cttattatca agattaaagc tcaatgcacc atttttacac ttaccatgac atacagtaca
     3481 tccattttca tattgacaag ataattcact aaacatcata tgtggtctta cagtccaact
     3541 ttctggattt gcacaccatt tacaactcag tggacatcca tttaaaaata cagttgttct
     3601 acaacctgga ccatcatgaa ctgaaaagct ttgtacatca aaaatcatgc cttctaattg
     3661 cttttgacta ctcattaaac acatgtcctt cacaagtaat tgcattcaat gttgaatata
     3721 cccagtcttc tttctctagg ccaacacatt tgcctatacc ttcatcatta gcttcaacaa
     3781 aatttttaca atttttacat tttggcatta cttttatctc tggacatgct gcatcatcta
     3841 aattaatcat ttgttttgat aatctacaaa ttcctttggt tgcatctact gcacaaaaat
     3901 tcatacaatc actatgcttt ctcatatttt acaccccttc atactctgtt ctagcaatta
     3961 cttcatcttg tattggttta cctaattcac accaatattg agtaaaccca gcaacacgta
     4021 ccattaattg acgataattg tcaggatttt tttgtgcatc ttttaatgtt tctgaatcta
     4081 caacattata ttgaatatgg aacccacctt ttcttaaata acttcttgtt aaatctaata
     4141 attttttagt tccttgttgt ccttttatag tagttggatg aagttttaaa ttcatctgag
     4201 aattttgaac tactgcttgg tcccaaatag ttgcagattc aaataatgca tatactccat
     4261 ttttatctgt tcctgcatat gcagagactg aaccatctga atatgttgtt ccacttagtc
     4321 taccatctgg agtagccaag gtagcagctc cctgtggccc atgtgtagaa acagatattt
     4381 ggcaagggta catcttctta gcatataaag attgtgcttc ttcacatact tttgataacc
     4441 atacctcata gtttttaaga atattatctg catacaaatc attatttcca tatttaggtg
     4501 catcaagaca ggccttataa atagcatcat atttaccagt cttatctact tttacttggt
     4561 ctgccataga ataatttcct acttctaatg catctttaaa tccaaaatta tttaatatgg
     4621 catccttcat ttcctctatt gtaaatgctt tgtcatcata tacaagtttc ttaagtgctg
     4681 caaatgaatt gaccatagta acagtaccac aagtttctac gtttaatgtt gcattataac
     4741 gatatcccat atttcctata tgttgacctt tgtctaaaca gtctggcttt agtagtgaat
     4801 ttattataga agtatcaaat tttctccata tatctaactc aatgttattt gctctttcta
     4861 aaacatttat tgcttgttta taataatctt taaatacttc tatgacttct tcataagtat
     4921 ctaatggctt attatgaggc tcaaatactt gtatattcat acgatagtct ttaccattca
     4981 taagaacaag ttctaaaatt tttggattag ctataaagtg aactccagag ccagcagatt
     5041 gacctgctcc acctgcaata gagtatgttt ttccgtttaa agttaactgt ttccaacacc
     5101 ctggagacgt ttccaaacat ccacctaatg caaatgctct tgcttcttca acagtcatac
     5161 cttcatcagc aaattgtttc atcataaatg ttgttccatt tgagttgttt acccatgctg
     5221 gataaccaga accaagcttt gtacattcag cagctttcat taagaaatct tctggaagct
     5281 tttcatcata tagcatagtt aaactaggtt gaggagttct acatctcata cttgcttcta
     5341 ataataactc ttctagttca ttagctcctg tagaaccatc ttcttttaat ccacctatag
     5401 ataaagtatt aaatgtattg cctgatagta ctcctccatt tacgcccgct gaagcaaaac
     5461 agtcaataca agtgattttt attctataaa gctctaataa ttcgataacc tcttcttttg
     5521 taattagtcc tttttctata tcttgttcat accaaggata taaaatttga ccaaaacgtc
     5581 ctatagacat accagatata gcatcctcat ttactgatgc tatatgaatt gtataagtca
     5641 actgaagagc ttccctaaat gtacgaggtt gtttatgtgc aatatgttct aatatctcca
     5701 ctaaatctga gtattctttt ttagcttcta aatctgtctc tatactttct aaatattttg
     5761 catgttttgc ataatttaaa atccaagttt gtaatccttc aataacttga ataacagcct
     5821 cataaaaata tagacggtcc ataccaataa gcccatctcc agatgcattt cctgcaactg
     5881 cctttttaca ttcttttgcc atttctataa ttccatctaa tccataattt aaaggataaa
     5941 aataatttat aacttctcta ccttgaggaa gtgtataccc tgaatcaaac atgcatataa
     6001 gtgtactcat taggttttct tttaaatcat aatctggcat cattttttca tagtgaaaac
     6061 ctaaatcttc aacagacttt ccaacccatt cctttgccat ttttacaagt acaggtactt
     6121 cttcttttct cattccgaat ttaccagcta ttgaaacgac atttccaaaa ctttcagtaa
     6181 catttcctcc accagaccca aattttgtaa gttcatcagc tgtgttactg gctaatttct
     6241 ttgcctcttc tctcatttgt tctccttgag caacaaagaa gctttcactt acccatggca
     6301 ttggaaaaga acctctataa tggcgtgtct tttgcattac tattttctca ccaggtatta
     6361 tatttggtgt catatgtgaa aaagcagctt taagagcttt agctcttcta actactggta
     6421 tatcaccatc tgattttcta tattctctat tataccaata tggaaattcc atatcagcag
     6481 tagataatgt attataatat acatctaata agtcttctgc tcttttactt gctttttttt
     6541 cattaaattc acttaaattt tctttatttt gaaaatttga tttaatattt tttgcttcca
     6601 aaatacttct tattttgtct tctttacttt gactcatttc ttcccctcct taatctttct
     6661 ataattagta taaaactttt taagaatgaa aaaaaagggt atattaccca ctttttaaaa
     6721 taatctttct atagaaaaaa ataaacttga agcaaatcta agccaaagac aatctgtata
     6781 tcctaaaatt tgttgcaatt cattggaata taaagattat aaatagtttt acttcaagtt
     6841 ttaatatata ctatatttat ttttattata tttaatttca cttctactta cttttttgaa
     6901 acaatacttt tatatttaca aattcatcta actctactga tttatcttta aatttggaac
     6961 tgcatttaaa ctcatatcat gcgttttccc atttataaaa ttataatatc ctgcacaccc
     7021 tatcatagct gcattatctg tgcataaaat tagaggagga tacttaacag taataccatt
     7081 gtctttagca agctctgtta ttttagctct aagccccgaa ttagaagcaa ccccaccaga
     7141 aagagttatg atattgtatc ctttgtcttt tactgctttt agtgctttag tagataaaac
     7201 ctctactaca gcctcttgga aagatgctgc tacatcttca actactattt cttcattttt
     7261 cattcttttt ccatttagat aatttaatac agaagatttt aatccactaa aactaaaatc
     7321 atagctatct tcttctaaat aggctcttgg aaattctata gcatgtttat ttccattctt
     7381 ggccagatta tcaataatag gacctcctgg atagcctaaa ttcatagctc ttgaaatctt
     7441 atcaaatgct tctccagatg catcatctct tgtttttcct aaaatttcat acttgccata
     7501 atcttttact tcaaccaagt gagtatgacc tccagatact attaatgtta taaaaggagg
     7561 ttttaaatcc ttatgctcta tatagtttgc acttagatgc ccttctatat gatttactcc
     7621 tacaagtgga atattcaaag tatatgcaag tgctttagca taagatagac caaccaataa
     7681 agcacctact agcccaggtc catatgtaac tgcgatatgg tctatatcat taaaacctat
     7741 atttgctttg tctaaagctt cttgaacaac tatatcaata ttttccacat gttttcttga
     7801 ggccacttct ggaactacac caccaaattt tttatgtgtc tcgatttgag tagagattat
     7861 gtttgaaagt acttctctac cattttttaa aactgatgca gctgtttcgt cacaactact
     7921 ctctatagct aatgttatta tatcactcat aaactctaca cctcttttaa ttggttccac
     7981 attataattg catcttcttt attatcactg tagtattctt ttcttattcc acccatttta
     8041 aatccatatt ttctatataa attttgtgct attttatttg atgctctaac ttctaatgtc
     8101 atagatgcaa tattattatc tttacataaa tcaactatgc cttttatcaa cttgtcccct
     8161 atttttttcc ctctatagtc actatgaaca gccacattat ttatatgccc ttcatcaagt
     8221 ataagccaaa ttcccacata tccgacaatt tttccattta acttagctac tatatacctt
     8281 gctacttcat tagacaactc ttttctaaaa gactcttttg accaataatc ttcaaaacaa
     8341 tttttttcta cttcaaaaac ttcatctata tcttctgtag tcatttcttc tatttttata
     8401 tcatctacta ttagttttgt attatcttcc attatttagc ctcttcattt tttcatcata
     8461 ttgtacttca gcttgagatt ttctgatata cataggattt atcgtataac aagtatgtac
     8521 atctatattt ttattatatt tatttaatgc taaagagcat aagcttcctg cgttagatac
     8581 attattagct ggtgaaggta tttttatatt ttttatattc tctaacttgt ctttatattt
     8641 gtatacagct tctccaacta ttataaattc ttcattagtt gatacaattt catctactaa
     8701 agcatcaaac tctacaacat caacaccatc taattctatc atctctccat tttcaaactt
     8761 gtatttagct gaataaactt gatttttctg tgcatctaat atacaacata tctttctatc
     8821 acagaaattt atgttatttg ctagagattc caaagaattt actgctatta ttggtatatt
     8881 atttacatgg gccatagctt ttactgtagc catacctatt ctaagtcctg taaacgaacc
     8941 tggacctata catacagcta ataaatccat atcttttata cttaaatcac tcatactcaa
     9001 catattttct atcattggca taagcttttg tgaatgagtt tttttgttgt ttactgtaaa
     9061 ctcacatatt aagttatcat cttcaacaac tgctacactt gctgccatag aagacgtatc
     9121 catacctagt attttcatta tttaatcaac tcctcaacta tctctttata aaactctcct
     9181 tttggagtta atatcatttc tcttccttca tctttatatc tcaattctat attcaaatat
     9241 tcttttggaa gtatatcttc tattagatta gcccattcta ttatacagat tccatttgaa
     9301 tttacatatt catcataacc aatatcatac atttcatcac tactacctat tctataaaca
     9361 tcaaaatgat acaaaggtat ttttccttca tattcattta ttatagtaaa agtaggactt
     9421 gttatataat cttctatccc taatgaatct gccaaacttt gtgtcattgt agttttacca
     9481 gctcctaaat ctcctactaa acagataaca ctgccttctt ttaaaagttt acctaattta
     9541 tatcctattt ctctagtttt attttcattt tctaaatata tttttgccat caattcatct
     9601 ccattttgat ataatcgtca tgtgaattta tatcactgct taattaatta tagtataagc
     9661 ctagattatt ataaagtact tgtctttatc tttttttatc tttcactatt gatggctgta
     9721 tctacacctt atatacaatc atcaatagca atttttacat actcttttgc tttatggttt
     9781 tgcttcaatg cttctttaca tattctctca gattgctctt ttacatactt aagagactta
     9841 tagttctctt ttactgcttc aatacacatt ttttctgttt gattttttat atacataagc
     9901 atttttccat tttgtcttac aatctctata catatatctt ctgtatactt atctatgtaa
     9961 cttagtacat ctttaatatt atatttttta tttaaatatc ttatagcatt tatacatatt
    10021 tcttctgtct tattgttgac aaattgtata cttcttccat cttgtttgac agcttctata
    10081 catattttat cagtttgttt atttacatat tgtaatgcca taccattttg tcttacggat
    10141 tctatacaca ttttttccgt ttgatttttt acatatttta atgctttata attttgctta
    10201 atggcctcta tacataattc ttcactctgt tctctaacgt atgcaagaca cctgccatct
    10261 tttttaattg cttctaaaca tatccttctt gtttgagtct ctaaagcctt tagtatactt
    10321 acaacatcta tattggaaga atgacttatt gcttttataa taagcccttc tgttttattt
    10381 cgtacatact taagtgcatt acaatctttt tttatagctt caagacacaa gtattctgtt
    10441 tgttctttta catattcaag tgctttataa ttatacttta ttgcttctaa acataattct
    10501 tcggtttgat tttttacata ctctaatgcc ttgtaattct gccttattgc taaaatacac
    10561 ataccatcag tttgattttc tatatttttt aattcacgat aatcgtattt taccctttcc
    10621 atattttcaa tatattcatt agtgatatac tttaaagaat ctcctaattt tgtatcataa
    10681 ataccaatat gaatatcttt ccaattacta ttaaaataat accctttatc atctaaaaat
    10741 aattttttta tttttttaga taaatgcatg aatatacacc cctatcatta ttaaattagt
    10801 tattattaaa agtttgatta ttaatcttta tagtttttta ataataactt tattttttgt
    10861 ttattataaa ttcgctctgt atattgtaga tacctttttc atattttaat agttaatcgt
    10921 tttaattctt cctttatttt aaaatttaaa tttgtctaaa tatttcttat tttatataca
    10981 ttttttgata aattaataaa gccataatat atttaatata tcaatcacaa tataggactg
    11041 aagttataaa taaattatgg ctattcaata catttaattt tatattttct tgtctaatta
    11101 tttctttatt atatttccca tttttttata aagtggcaat gttactaaag atattataac
    11161 agccttcagc acattaaatg gagctatcat ccaaattaca aaagtgaata aatcatgaac
    11221 ttgaggattt atagctgaac ctaaacctat tatagcatct agtcccataa gttgaccata
    11281 aagtggcaac attacaaaat aatttactaa tgaaccaatt atagtcatag atattgttcc
    11341 taatacaaaa ccaatcagta caccttttaa atctccactt ctcttatatg aataacaaag
    11401 tatcattaca taagaaccac caattaatat atttgcaaat tctcctattc ctccagttgt
    11461 tgttgctgtt attgcttgta acacattttt tactacaact attccaaatc caactaatgg
    11521 tcctagagac attcctccga atattgctgg aatatctgat aagtcaattt ttaagaaatc
    11581 tggaaatata cctggtattg gtgcttgaat aaacattaat aaataagata ttgcagataa
    11641 aattgacatt actactaaag ttcttgttga aatcatattg cctctttttg ctgtgttttg
    11701 cataattatt ttccccctaa tcaatatgtt tatatttttt aggaaaaaca aaaaactcga
    11761 agaccctatc ttcgagtttt tatggatata gtttaaacaa actaaaataa acttatttat
    11821 cttccataaa tatcttctct catccagact ttactgtcgg ctttggaatc tcaccaaatc
    11881 aaccacctaa gtagttcgcg ggctataccg ccggtaggga atttcaccct gccccgaaga
    11941 tcttctttga ttttttaatt ttccttacac tcttatatta gttaattttt taataattgt
    12001 caagttattt tttctcatac actactttgc catttattat agtgtaaagt acattacttt
    12061 gaatctctag tggagaatta tcccaaatta ctaggtctgc atctttacca acttctatag
    12121 agcctactct atcttctatt cctaaagcct tagctggatt tatcgtaatt gattctattg
    12181 ctttttcttc tttcatacca tgttttactg caatacctgc acatattgga agatattgaa
    12241 ctggtattac tggatggtct gtcattatac atacatcaag acctgcattt gaaagtattc
    12301 ctgctgtatt aaatgtcaaa tttctaagct caaatttaga cctttctgac aaagatggcc
    12361 ctactattac aggaaaccct tcttccacta attcatctac aataagatga ccttctgtac
    12421 aatggtctaa tgtaagcttt aaattaaatt cttttgcaat tcttattgct gtaaacatat
    12481 catctgctct atgagcatgt gccttaaaag gaatttctct tcttaaaact ggtattaaac
    12541 tttccatttt tatatcatat tcaggttttt cacagtcttc atcatcatga ctctcataca
    12601 tatcaatatc ctctaaatat tcttctgctt tttttaggtt ttctctaagt aaagcagcta
    12661 tagccattct agtttgagga gatttatcat cctgaccata acagctcttt ggattttctc
    12721 caaaagcaat tttagatgca actggatttt ttattaccat tttatcaatt ctttttccac
    12781 atgtctttat agctatacat tgaccaccca taacatttgc acttccaggg gttgtacaaa
    12841 cagaagttat tccaccttca aaagcttctt taaaagttct atccattgga ttaattccat
    12901 ctataggatt taattgtggt gttattgggt cagtctcttc atttccatct gctccttcaa
    12961 accctatacc atcttcccat aaacctaagt gagtatgagc atctataaat ccaggaaaca
    13021 caagtttatt actagcatct attacctcta catctaaagg agctatcaaa tcttctccaa
    13081 tttctattat ttttccttca tctataagta tatctccatg aattatccca tttgttatgg
    13141 tatttatttt accatttttt ataaaaatca ttcattttcc tcctgatttt caagactatc
    13201 gttcttattt taatatattt tcttactata tacttttaca aatttttaac atttatacac
    13261 tcgagtaatc ttatttacaa atatagaatc taagtactat aaccatacat aaacttatct
    13321 attttttcat tgataaggca actctttttt tgtttaagtc aattccaatg acttttacat
    13381 ctactatatc acctactgta acaatactca tagggtcttt cacaaaacta ttactcattt
    13441 ctgatttatg aactaatccg tcatttttta ttccaatatc tacaaatgca ccaaagtcta
    13501 caacatttct tattgtacct tttaaagtca taccctcttg tatatcttca atttttaaga
    13561 catcagttct aagtataggt tttatacctt cttctcttgg gtctcttcct ggttttttga
    13621 tttcagctat gatatctttt aacgtaactt gtcctacttc cagtttttca cttaattccc
    13681 tcaagccaat ttcttttatt ttttcatcta tttcacctat attcttattc tttacatcat
    13741 ctaaagaata tccaattatc tcaatcattt ttttacatat atcataactt tcaggatgca
    13801 cacctgtatt atctagtgga tttttaccct ctaagattct cataaatcca gcacattgag
    13861 taaatgcttg aggtcctagt ctttttactt ttttcaattg tgctcttgac gtaaaatctc
    13921 cattttcttc tctatatgct attatatttt ttgctatagc tttacttatt cctgcaacat
    13981 gttctagtag ggagtatgat gcagtattta aatctactcc aacactattt acagaatctt
    14041 caacaactcc atctagtact tcttctaatc tctttttgtt taaatcatgc tgatattgac
    14101 caacaccaat acttttaggg tctattttta caagctctgc taatgggtct tgtaatcttc
    14161 tagctataga tatagctcct cttattgata catttatatc tggatgttcc tcatttgcaa
    14221 gttcagatgc tgaataaaca gatgctcctg cttcactaac tatcacatac tgcacttcgc
    14281 tatctatttc ttttatcatt tctgatacaa aagtttctga ttctctagat gcagtaccat
    14341 ttccaattga aattatatct atatcgtatt tttcaatcaa tccttttaaa actttctttg
    14401 ctccctctac atcattttga gggtctgttg gatatactgt tgtataatct agtagtttac
    14461 catttttatc tactacagct attttacatc cagttctaaa tgcagggtca aatcccataa
    14521 ctactttgtc tttaactggt ggttgaagca ataaactttt aacattcttt ccaaatacac
    14581 ttatagctct ttcttgagct atttctgtta aatgatttct tatttctctt tctattgatg
    14641 ggaaaattaa tcttttataa gaatcttcta ttgaacttac gatttcttct ttatttttga
    14701 aatttttatc atttacatat tcgtttatta tgtaatttaa aactttatca ttatttattt
    14761 ccaacttaac ctttaggaag ctttcttttt ctcctctgtt tattgccaaa actctatgtg
    14821 gagccatact ttttactgct tcgctataat cataatacat atcataaacc gatttctcat
    14881 cagtagcact tttgctaact atcatacctt ctcttaaagc taattctctt atatactttc
    14941 taatcttagc atcatctgag actagttctg caattatatc tctagcacct tttagagcat
    15001 cttcaataga aagaacttct ttctcttcat caagataatt ctttgcctct atttctatat
    15061 tatctaaatt attctctagt attgacaatg ctaaattttc taatcctttt tcttttgcta
    15121 ttgttgctct agttcttttc ttttgtttat atggagcata tatatcttct acttcttgta
    15181 atgtttttgc tttttctata ttttgtgtta tttcatctgt taatttccct tgttcatcta
    15241 ttagtctaac tacatcatct tttctacttt gaagatttct caaatacatt aatttttcat
    15301 aaaattctct tagtgtaaca tcactcattt cgccagtcat ttcttttcta tatctagcta
    15361 taaatggtat tgtatttcct tcatcaataa gttttaatgt attatttatt tgttcatctc
    15421 taagattaaa ctcttttttt aagatttgat ttatatccaa tttattcact cctaatgtta
    15481 gtttttcata agtataacaa aacagaacga aggctgaggg ttatcgtcct cagcctaagt
    15541 gctgtaagtg catattaagc actaaccttc ttatacatat atcttaatta cagtgcatat
    15601 ttattaaact attttattca tttttaagta ttcattaata aacaagtcta tatttccatt
    15661 cattacacta tctacatttc caaactcagc atttgttcta tggtctttaa ccaatttata
    15721 tggttgaaaa acatatgacc taatttgact accccaagtt atttgtgagt atttgccttg
    15781 tatatcttct attttttcct tttgctctaa ctcttttaat tctatcaatt tcgccattaa
    15841 aagtctcata gctctgtcct tatttagatg ctgagacctt tcattttggc actgaactac
    15901 tacaccagta gggatatgtg ttattctaac tgctgaatca gtcgtattta catgttgccc
    15961 tccagctcca gaagctctat atgtgtctat ctttaaatct gatggattaa tttctacttc
    16021 tatattttca tctaactctg gtgttacatc tatagatgca aatgaagtat gtctttttcc
    16081 tgatgggtca aatggagata ttctcactaa tctatgaact cctttttcac tctttaaata
    16141 cccataagca tttgttcctt ctacaagaat tgtagctgtc tttatccctg cttcagggtc
    16201 tgatattata tctaaaagct taactttata acccttactt tctgaccatc ttatatacat
    16261 tctcaaaagc atttgagccc aatcttgagc atctaagcca ccagtaccag catttataga
    16321 taatatagca ttatttttat catactctcc atttagaaga gtctttattt tcatttcaga
    16381 taatttttct tccattgatt ctatggactt ttctatatct ctttctacag aatcatcatc
    16441 ttcttctaaa cctatttcaa ttaatacttc tatatcttct aataagcttt ttaagctttc
    16501 atattcttct aatgtctttt ttaaagattt attttcttga agtactcttt gagcgacttc
    16561 attatcattc caaaaatctt gttgattcat cttttcttca ttaatttgta tatttttcat
    16621 caatgaagct atgtcaaaga gaaaccctca attcttctaa atttgaagcc atctgctcca
    16681 tactatgtct atactcttgt aactttaaca tttaatcctc cgagattatc ttccacaaca
    16741 gtttttatat tttttaccac ttccacaagg gcataagtca tttcttccaa ccttttcatc
    16801 ttttttaaca gttttacttg ttggaagtgt tccatctgat ggagaagata gattttcaac
    16861 atctacaact gcttttcttt ccactggagt ttctattgtt atattaaata aatatcttac
    16921 agtatcttct ttgatatgtt tattcatttc atcaaacata tcaaaacctt ccattttata
    16981 agcaataact gggtcttgct gacctacagc acgaagacca ataccttggc gtaattggtc
    17041 catagcatct atatggtcta tccaatggtt atcaacagct tgaagtaaga ttactctctc
    17101 aacttctctc attctctcat agccaacttg ttcttctttt gaagtatata tcttcatagc
    17161 tatttcataa actttttctg ttatttcaac tggattaagt ttttctattt ctggtatttc
    17221 tatgctacct tttggcaaga acagattata catatgttct ttgaatcctt cttcatcaaa
    17281 tcctttatct tgtgtataaa gagtaactgc ttcttctatt atagaatgag tcattgattg
    17341 tatttgttct tgtaaatctt caccttctaa tacacgtttt ctctctgcat atattatttc
    17401 tctttgttta ttcataacat catcatattg aagtacatgc tttcttattc caaagttttt
    17461 accttcgact ttcttttgtg ctccttcaat agacttagat aatattctat gttctatcgg
    17521 catgtcttct tcaagaccta ttttatctac aataccagat attctatcac taccaaacaa
    17581 tctcataagg tcatcatcaa gacctatata gaatcttgaa ctccctgggt caccttgacg
    17641 tccagcacga cctctcaact ggttatctat tcttcgagat tcgtgtcttt ctgtacctat
    17701 tatagcaagt ccacctgctt ctacaacttg tttttgttct tcttgtgttt gttgcttaaa
    17761 cttttcaaat agtttttcat actcttctct agcttcaaat agaatttcat taccttttac
    17821 aggtatacct tctattggag tatctactct atttactatt tcttctttaa atccatttct
    17881 tctcatttct ctcttagtta aaaagtctgg atttccacct agaactatat ctgtaccacg
    17941 accagccata ttagtagcta ttgtaacagc tccaagtcta ccagcttgtg ctattatttc
    18001 tgcctcttta tcatgatgct tagcatttaa tacctcgtgc tttatgccct ttttctttag
    18061 tatttgtgat aataattctg atttttctat tgatactgta cctacaagta ttggttgatt
    18121 gactttgtgc ctttcaatta tttcttgtgc tacagcatta aatttaccta tttcactttt
    18181 gtatacacag tctggtaagt cttcccttat cataagctta ttagtaggta cttggaaaac
    18241 atccattttg tagattgctt taaactcttc ttcttctgtt tttgctgtac cagtcatccc
    18301 agaaagtttt ttgtacattc taaagtaatt ttggaatgtt acagtagcta gtgttttaga
    18361 ttctctttgt attttaagac cttctttagc ctctattgct tgatgtaaac cttctgaata
    18421 tcttctacca aacataagtc tacctgtgaa ctcatctact attactattt ctccatcttt
    18481 agcaacataa tcaacatctt tcttcattat tacatgtgct cttaaagctt gatttatatg
    18541 atggtataat tctgtatgag atatatctgt tatattatca acattaaaat acacttctgc
    18601 tttttgtata cccgatgctg ttagagatac agccttatct ttttcttcta attcataatc
    18661 atctggtttt aaggttaaaa caaatgtgtt tgcatctgaa tataagtgag ttgatttgtc
    18721 tccaggtcca gatatgataa gtggtgttct agcctcatct attagtatag agtcaacctc
    18781 atccactata gcataattta atcctctttg aactctttgc tctttgtgta ttaccatatt
    18841 gtcctttaga tagtcaaatc catattcatt atttgtacca taagttatat cacaatcata
    18901 ttgttctttt ctaacttttg gattttgacc gtgtataata acgcctacac tcattcctaa
    18961 aaactcatag atttttgcca tttggtctct atcacgtttt gcaaggtaat catttactgt
    19021 tacaacatgt acaccttttc cagtaagcgc attcaaataa acaggagcag ttgcaaccaa
    19081 agttttacct tcacctgttt tcatttctgc tattcttcct tgatgtagta ctatacctcc
    19141 aatcatttga actctgtagt gtctaagtcc taacactctc ttagaaactt ctctaactac
    19201 tgcaaaagct tctggaagta tatcatctat actttcacca tttgctaatc tttctttaaa
    19261 tatatttgtc atatttttaa gttccgagtc tgccatagat tcaaatttag gttctaatga
    19321 atctattata tcaacagttt tattgaactt ttttaattct tttttatctg ccatgttgaa
    19381 taaattatcc ataaagctca tattttacat ctctcctcgt tctttattgt actattattc
    19441 attttaccat aaaaactata cttttaatat taatttaaaa agctatcgat aatttattta
    19501 tcgatagcta atatgattaa atctaaattg ctttattcat gttctattat accatatcca
    19561 ccattattac gcttataaac tactgctatt tcatctgaat ctatgttctt gaacatataa
    19621 aaatcatgtc ctattaattc catctgtaat acagcttctt cttcactcat tggcttaaca
    19681 ctaaatttct tacgtctttg aattactaat tcttgatttt catccccata attctcttgt
    19741 tcatcagcta gatttatctc tgcatcttgg aatcttatac tcttgttgtc tttatgttta
    19801 tcttgtaatc tctttttata tttttttaat tgtttactta atttatcata tacaacatct
    19861 atagctgtgt ataaattttc ctgcatatct tctgctctaa ttataggacc atttacagta
    19921 gctattgtta cttctatttt ttgtcttgct tttctcgcac ttactgtaac ttttacatca
    19981 gtgtctggat gaatgtaatt atctaactta cttagtttag tttcgatttt attttttatt
    20041 gcatcagtta attcgatttg ttttccagat ataattatgt tcataaatac atctcccttc
    20101 aattatgcca gtataagaaa atttatactt taataatata ttctacacga actctcataa
    20161 tcctcttttt attatttact tttatgattt tttctattca attgatagaa ttatttatca
    20221 tttttagtta cattgatact tatgtggatt aatattatta caaacacctg tggataatgt
    20281 ggataaaact gttaataact cttgtttttg tttatttaaa gctattttta ctcctatttc
    20341 tatccacaaa aatctccatt aattgtttat taatatgtga attcatgtca taagatggta
    20401 attttaggaa attatgtgta ttatatgcca atataccttt ataatttttt aataaatata
    20461 aaccctctaa aacctccata atctatcttt tctttcatta tttatattta aaactaatta
    20521 aaagttataa agcatactca acaataattt c
//
LOCUS       DAELTA010000066        20379 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.066, whole genome shotgun sequence.
ACCESSION   DAELTA010000066 DAELTA010000000
VERSION     DAELTA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.066"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..551
                     /locus_tag="KPG13_001974"
     CDS             <1..551
                     /locus_tag="KPG13_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0985707.1"
                     /translation="FCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKAR
                     LKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWS
                     EFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDLNIREWICPSCNETHD
                     RDINASINILKEGLRLITIQNK"
     gene            669..1079
                     /locus_tag="KPG13_001975"
                     /pseudo
     CDS             669..1079
                     /locus_tag="KPG13_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(1379..1966)
                     /locus_tag="KPG13_001976"
     CDS             complement(1379..1966)
                     /locus_tag="KPG13_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG0985708.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(2052..2489)
                     /locus_tag="KPG13_001977"
     CDS             complement(2052..2489)
                     /locus_tag="KPG13_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0985709.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            2593..3591
                     /locus_tag="KPG13_001978"
     CDS             2593..3591
                     /locus_tag="KPG13_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG0985710.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3711..4637)
                     /locus_tag="KPG13_001979"
     CDS             complement(3711..4637)
                     /locus_tag="KPG13_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0985711.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            5022..6626
                     /locus_tag="KPG13_001980"
     CDS             5022..6626
                     /locus_tag="KPG13_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0985712.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            7442..8713
                     /gene="serS"
                     /locus_tag="KPG13_001981"
     CDS             7442..8713
                     /gene="serS"
                     /locus_tag="KPG13_001981"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0985713.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            8991..10496
                     /locus_tag="KPG13_001982"
     CDS             8991..10496
                     /locus_tag="KPG13_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0985714.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            10610..11308
                     /locus_tag="KPG13_001983"
     CDS             10610..11308
                     /locus_tag="KPG13_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0985715.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            11593..12681
                     /locus_tag="KPG13_001984"
     CDS             11593..12681
                     /locus_tag="KPG13_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985716.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            12920..14110
                     /locus_tag="KPG13_001985"
     CDS             12920..14110
                     /locus_tag="KPG13_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0985717.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            14201..15703
                     /locus_tag="KPG13_001986"
     CDS             14201..15703
                     /locus_tag="KPG13_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0985718.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            15775..17031
                     /locus_tag="KPG13_001987"
     CDS             15775..17031
                     /locus_tag="KPG13_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG0985719.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            17203..19977
                     /locus_tag="KPG13_001988"
     CDS             17203..19977
                     /locus_tag="KPG13_001988"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0985720.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(20042..20227)
                     /locus_tag="KPG13_001989"
     CDS             complement(20042..20227)
                     /locus_tag="KPG13_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG0985721.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
BASE COUNT         7846 a         2345 c         3476 g         6712 t
ORIGIN      
        1 aattttgtat ctcaagttgt ggagacttta tagaaaatcc taagtatctt aagaagtcat
       61 taaacaaact tgctaaatta caaagagaat tatcgagaaa gacaatcggt agtttaaata
      121 gaaataaagc aagattaaaa gttgcaagac ttcaagaaca tatagctaat caaagaaatg
      181 attttctaca aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag
      241 atttacaagt aaagaatatg attagaaatc gtaaactatc tcgcttaatt tctgatgtgt
      301 catggtcaga atttgttcgt caattaaaat acaaggctaa ttggcatggt agacaaattg
      361 taaaggtagg taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg
      421 aggaaatgaa ggacttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg
      481 atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta ataacaattc
      541 aaaataaata acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt
      601 aagacatatt ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg
      661 aggttcagga tattgcaaat atgtttatga aaaaggatat taataaagaa tttgacattg
      721 attttattct cagtaaagaa gttatatgta attataccaa agaaatattt caaaatttat
      781 ataaaaaaga acaaagcata ggtaaaaaag aactgcttat attgagcata atagagaagt
      841 ttttagttaa atctataata acttttgaag agttaaaatt gatagtgaat ataatagaag
      901 aaaattttac atcaatatac aatgaaagtg gaaagattta tttgtttaga aagtttggag
      961 ttccattcgt agtaggatgt ctggattata aaaaagtaaa gtttgaaaaa gatgtagtta
     1021 agattattga ggttgacttg acaaaagaaa taaatgatat aagtataaag ttatcatagt
     1081 gtattatctt atataatgta ttataataat ctacttaaaa tacactttat ttaaatattt
     1141 agtttaaaga tgagatttta tgtgagtgtg taaaagttaa agatagattt aagtaggtta
     1201 tagggattag taaacaataa aatgaaccat gatgaactac tataaattgt atttaaacta
     1261 cattttctac ttatagtaat atcaatggtt cattttattg ttattaagat aatacagtat
     1321 ttataaaaat acttgatata aatatagtaa attgtttaaa tagaattatt gccttaattt
     1381 attcaaataa tgagttttta aaatctaaga aatatccttg tggatgaaga catacaggac
     1441 aagctttagg agcttcttta cctatatgta tatggccaca atgtgtacat atccattgta
     1501 tatcttcatt tcttctgaat aaagtacctt cttctaaatc agtagcatat ctaccaaatc
     1561 tatcaccatg aactttttct atttcagcta tattttcaaa taacttagct atagattcaa
     1621 atccttcttc acgtgctgtt tttccaaagt ttttatatac atcatcccac tcttgaagct
     1681 cattatgttg tgcagcttta aggtgttgtg ctgtcttgtc atataaatct acagggtatg
     1741 ctcctgatat acttatattt tcaccagcaa attcttttaa tttagaaaag aattgttttg
     1801 catgtgattt ttcttggtca gcagtgtaat taaataaaga ctctattata tataatcctt
     1861 ctttttttgc tacagatgca gaaatattgt atctatttct agcttgactc tcacctgcaa
     1921 aagctcttaa taaattttct ttagttttac tatcttttaa attcatattc tacctctcta
     1981 tcacaataaa tttttacaat atttgattct ttttatttat tattccaata ggagatataa
     2041 ttatgctcaa tttagtcagc atttttaaaa ttatcattaa ttttttttaa aattctaaga
     2101 aattcttgtt tttcattttc tgtaatatta tggtatgccg tctctttaac ttgtgttgag
     2161 atgctattaa aagtatcttc tataagcagt gccttttttg taagctttat atacgtgata
     2221 cgtttgtcat ttgtacattt ttgtctttct aaatatccta ggtttataag tttattaacc
     2281 aacactgtaa cagtggattt atcttttcct attttagctg caatttcttt cattgtaagt
     2341 attccattat tttcataaag tgcagttaaa atatttccat gagtaggtat taactcacct
     2401 aaaccatttt tactgagttt attttcaatg aatttaatca tactagcctt tgttttactt
     2461 atgaaatgta caatatattt atcttccata aattattctc caatctttaa tctatttaaa
     2521 ttagtttgac ataaaactat ttataaacta taattagttt aacataaaac taaatgtatt
     2581 gggaggaaat ttatgaataa aaaatttaaa tcactatata ttacagaaga agaaggaaat
     2641 tttagaagaa atatagttga aagagaagtg gatacattgc cagaaggcga agttctaata
     2701 aaagtaaaat attcttctct aaattacaag gatgcacttt ctgcaaatgg taataagggt
     2761 gttacgagaa agtatcctca tacaccagga attgatgcat ctggtatagt agaatctagt
     2821 agttgtgatg attttaaaat tggagaagaa gttttagtaa caggttatga cttgggaatg
     2881 aatacttctg gtggtttttc agaatacata agagttcctt ctagttgggt agttaaactt
     2941 cctaaaaatc tttcactaaa agaagcaatg gattatggta cagcagggtt tacagcagct
     3001 ttatctgtgt ataaatttat agattctgta gacaagaata tgggagatgt attagtcact
     3061 ggtggtactg gaggagttgc tgtaattgca actaaaatat tgattaaatt aggttattca
     3121 gttgtagttt caacaggaaa attagaagaa caaaaagaag tattactaaa cttaggtgtt
     3181 aaagatgtta tacataggtc agaagtggat gataactcag gaagacctct gcttagacca
     3241 agatgggctg gagtaataga tacagtagga ggaaatactc tagcaacagc aataaagaca
     3301 actaattatt gtggagcagt aacaacttgt ggtaatgcag gaggagttga ttttacaagt
     3361 tcagtatatc catttatctt aaagggaatt actttatatg gtatagattc tgtggattgc
     3421 ccaatgaact taagattaaa aatatgggat ttattatcaa atgaatggaa attagatgat
     3481 ttagatgata tggtaaatgt agtatcattg gaagagttaa atgatagtat agattcaata
     3541 atggcaggaa aacatgttgg aagaacagta gttgatttag aaaaaatata ggtgaataaa
     3601 tttataatta ggattatagg ttttaagcaa aaatgagata cttgaaattt acttgtgagt
     3661 atctcatttt tttatgtaaa aagatttatg tagatgtttg tgtaaaaaga ttattcatga
     3721 acaattaatt caaatacatc tggtcttgaa tagtgacctt ctgaatcaaa atctagtcta
     3781 ctaagtacta ttttttctag gtctaaatca gctattagca tttcttcttt attgaaaata
     3841 ggacctgcta catattttcc aaaagggtca actatacaac ttcctcctgg acacataatt
     3901 tctggttctg catctagctc tttatagtag ttaaggtcag ttggatacat atttttttca
     3961 acatattgat tgcatccaat tacaaaacat cttccttcta aagcaatatg cttcatagtt
     4021 gcttgccact cttctcttga atctgcagta ggggctatgt aaagttttac acccttagca
     4081 taaagagctg ttctagctaa tggcatataa ttttcccaac aaatcaacga acccattttt
     4141 ccatatggag tatcaactac tgtaagagta cttccatctc cttctcccca aatacatctt
     4201 tctgttcctg taggtttaag ttttctgtgt ttaccaatta gcttaccttc tggagagaaa
     4261 aataagtttg tgcaataaag ggtacaattt atatcattac catctctttc tgttataccc
     4321 attgatagat atactcctgc ttcttgtgct gcttttccta ataaatctgt tgtagcacta
     4381 ggaactggta cagaattatc atagtatctt ttccaatctt ctctaccttc cattgttcta
     4441 cttccaacaa caaatccaaa tgaaagacct cttggatatg caggtatgaa tgcttcaggg
     4501 aaaactacga tatttgcccc ttttctacca gcttctttaa ttaatcctaa tgctttttca
     4561 acagtttttt ctaaatccat tatgactggt gacgcctgga caacagcaat tcttacattt
     4621 ttattttcct tgctcataaa aacctcctaa aaaatattta aattttttgc attgaataga
     4681 ttacatgaga tgttatttga gaactaagat acttatattg tgaataataa tgtaactgta
     4741 tatttaaaat aatacatcat gatttaaaaa attacaattg tacagatata attgagttta
     4801 gatatttaaa tttaacaact tataatattt tttcaattag tataaagcct attaatttgt
     4861 cttatttaca atgaatagta tttacttctt ggcaaacaat aattaaaaat aaaactttta
     4921 tatgattaaa ctctaaagaa aaagtttata aagaagataa tataattaag cagaaaataa
     4981 tgaaaaaaat ttattaaagt agacaacgta aggagaattt tatgaaagaa aaaatttatt
     5041 acaatggaaa tatcatcaca atggaagatt caatatgtgg agatgctatt cttataaaag
     5101 acaaaataat aaaaaagata ggaacaaaag aagaagtatt tgctttgaaa aataaagata
     5161 cagaaattat agatttacaa ggtaagacat tgatgccatc ttttatagat tcacatagtc
     5221 acttgatagc ttttgctact actctaaagc tagttccttt agaagatgca accagtttta
     5281 aagacatagt aaaaaaaata caggatttca aagagtcgaa taatataaaa aaaggagact
     5341 ggataattgg attttcatat gataataatt ttttagaaga aaataagcat ccagataaga
     5401 gtgtactcga tagtgcatct tcagaaaatc caatactaat ttcacatgct tctggtcata
     5461 tgggagttgc aaatacattg gggttaaaac agcttggtgt taccaatgaa acacgtgacc
     5521 cagaaggtgg acatataggt agagtagaag gcagtgaaga accaaatgga tatttagaag
     5581 aaaatgcatt ttttaatgta gcatctaaaa taaaacaacc atcatcaaat gagattttta
     5641 attcaattga aaaagcacag aacatatatc taagctatgg tattacaaca gctcaagaag
     5701 gtttaatgga agaaaatcaa tttaacattt taaaagcaat ggcaaatcaa aataaattaa
     5761 aaatggatgt tgtaggatat gtaaatttga aaaaaagtaa gtctgtagca gataataata
     5821 gggagtttat aaaaaaatat ataaatagat ttaaaatagg aggttataag attttcttag
     5881 atggttctcc acaaggtaag acagcatggt tgagtagacc atatgaaaat tcagatgatg
     5941 gttattgtgg atatcctata tacaaagatg aagaagtaga aaagtttata gatatttcac
     6001 taaaagaaaa gatgcaactc ttaactcatt gtaatggaga tgcagcagca gaccagttga
     6061 tagatgcgtt tgagaaagtt ttaaatttaa aagagcaaag tagtgaaaat catataagac
     6121 cagttatgat acatgctcaa acagtaagag aagaccagat tgatgatatg aaagtaataa
     6181 atatgatacc ttcatacttt gtagctcaca catattattg gggtgatatt catatcaaaa
     6241 accttggaga agatagagcg tttaagatta gcccacttaa aacaactata gaaaagggat
     6301 taatttacac tcttcatcag gatacaccag taatagcacc taatatgctg gaaacagtat
     6361 ggtgtgctgt caatagaatt acaaaaaaag gtatacaaat aggagaaaat gaaaagataa
     6421 gcccattaga tgctttaaag ggtgttacta taaatgcagc atatcaatat tttgaagaag
     6481 ataaaaaagg ttcaattaaa gaagggaaat tagctaattt gataatctta gatgaaaacc
     6541 cacttacaat cgaccctatg aaaataaaag atattaaagt attgcagact ataagagaag
     6601 gagaagtatt gtattctttg aagtagatga aagttagata ttttataatc tataaaaaat
     6661 agttttatat aataagttag gtagaattaa ataagattac agttaaatca acaattatat
     6721 ttttattaaa ttttaattat tgtattgaag atttataagt ataattttat tgattctacc
     6781 tttttaaata actgtcgatt tattaaagtt atctatgtac tatattaaac aattaagtag
     6841 ttattaattg aacttattag tttattaaaa aactatgtat attactttgc agtaagactt
     6901 tggaactaaa tctataatag caaatttaaa tttattaaat agattacctt ataacatttc
     6961 atataaaata tattgtgaat ctgagaaaat tactattttt ctttgagtat tttttgtaag
     7021 cagttctaaa agattcaatt tcatacctat tacttgaatc attcattaaa ataattctaa
     7081 tttaaaaaat aattctatcc aaataataga tatataggac tatgttataa tataaaaaaa
     7141 tttatataaa agttaataac tatacaagat tgataaagaa aaagtcaaaa tagataattg
     7201 attagcaaaa tagatataat tataatgaag ttaaatctag caatattctc ctaaaattaa
     7261 attacattta tttaattaaa tataatttga caatggacaa aaaagtgagt aaaatataaa
     7321 taaaataaaa ataacaggtt cggataaaga aaaaggtttc ttaacctcaa gcataaagta
     7381 tattatctaa gttatttgag attaagagat aaatccaatc ttaggtactg aggaggaaaa
     7441 aatgcttgat ataaaaagaa taagagaaaa tttagattgt ataaaaaaag caatggaaag
     7501 aaggggcgaa aaagacttta accttgatga agtagttaag ttagatgatg aaagaagaaa
     7561 gattcttcaa gaagttgaag ttatgaaaaa tgaattaaat acagcatcta aaaatatacc
     7621 aaatcttata aaagaaggta aagatgtaga aaatgaaaag attaaactaa aagagttatc
     7681 tgataaaatt aaggtaatag accaaaatct taaagaagta gaagataaga tggaatacct
     7741 attaatgaga ataccaaatg taccacatcc agaagtacca caaggtgaaa ctgatgaaga
     7801 taatgttgaa gtaagaactt ggggaaagac tactaccttt gattttgaat ctaaggctca
     7861 ttgggaaatt ggaacagaat taggtatact tgattttgaa acagctagta aaataactgg
     7921 ttcaagattt actctttaca aaggattagg tgctagatta gagagagcat tattaaattt
     7981 ttacttagac acgaatacaa aagtaaatgg atatacagaa gttattccac catttatggc
     8041 aaatagaaat agttttttag gtacaggaca gttacctaag tttgaagaag atatgtttaa
     8101 aatagaaggt atggactatt ttatgattcc aacatcagaa gttcctttaa ctaatataca
     8161 tgctaatgaa atacttaaat ttgaacaatt accgataaat tatacagctt atactccatg
     8221 ttttaggtca gaagctggtt ctgctggaag agatacaaga ggtttagtaa gacaacatca
     8281 gtttaacaaa gtagaaatgg ttaaaatagt tgcaccagaa gaatcttaca atgagttaga
     8341 aaagcttact aacaatgcgg aaactatgct acaattattg aatctgcctt atagggtagt
     8401 aaagatatgt acaggtgatt taggatttac ggcttctttt aaatatgatg tagaagtttg
     8461 gatgccaagt tacaataggt atgttgagat ttcatcttgt tcaaactgcg aagatttcca
     8521 agctagaaga gcaggaataa gatttaaaag agataaagat tctaaagctg aatatgtaca
     8581 cacattaaat ggctctgggt tagctattgg aagaagtgtt gcagctatat tagaaaatta
     8641 tcaacaagaa gatggttctg tggttgttcc tgaagtttta agaccatata tgggagtttc
     8701 tgttattaag taagagttaa attatgtatt tttaagcaat acaataatat agaggtaata
     8761 aaaaataatt aatataaaag tgatacaata atatagaagt aataaaaaaa tagttaatat
     8821 aaaagtgata caataatata gaagtaataa aaaaaaataa ttaatataaa aatgtgtaca
     8881 aactaatgaa tattgatata gtaagctatc atatgaaaga gtatgtacac atttttttta
     8941 ctaatttttc acaatatgaa cataaataat ttacaggaaa ggaatctttt atgaaaattc
     9001 aaaatactga gtggggatat attgaatgga aacatactta tgatgaaaat aatccaaaac
     9061 aagctatgaa tatatacatt gcagtaacta tgcctggtaa gaaacatttt aatcatgttc
     9121 attatggtca agagcagatg atatatattt tagagggaga gggtttgtat attataaatg
     9181 gagtttggaa gccattttat caaggtatga ttttttatat agaatctggt agcacacatg
     9241 aaactataaa tacaggtgat agagaaataa aagaacttat tgtttcaaat aatgtagatg
     9301 atgtaggaga gagtgaagtt atagatatta atcctaataa ttatttaaag aagaccttga
     9361 taaattatag tgaaagtaca ttaaatttgt atgcagcagt agaatcaatt cgtggtcaat
     9421 ttatagaccc gtttaaaatt ccattaatta tatatgatga ttcatggaat atagttttaa
     9481 aaaatcctta ttttccactt ttttgttttg aaaaatgtaa tcctatgaaa tttcctcaaa
     9541 attgtgattg tatgaatcaa aaaagtagca atcaatttgt atgtgaatat gggataacta
     9601 tatataatat accaatactg tacaaaagca atagcatagg tgttataaga ggagggtatg
     9661 tactattatc tgatttgaat ttagatacag agcataataa tttatatgat attccagagg
     9721 gagctgctag aagtataaaa cgcctcttga aacaaatatc taaaaatata ataaattttt
     9781 gctcttttaa tgatataaga aaggatttac aagaaaaaga gaagactata gcaagaacat
     9841 atcattatgg agagcaactt gaaatgaatt taaaagtagc tcaagatatg gttactaatt
     9901 taagaataaa tcatcacttt ttatttaata cattaaattc aatggcaagc atagcactag
     9961 atgatggtag ttatgatttg tatagtgcaa taatagattt atcaagaatg tttagatata
    10021 caatgaggtc tgatttaaga tttgtagaat tagaatctga aatattatac attaaaaatt
    10081 atttgaattt acagaaatta cgttatggag atgcacttaa agtaaaatat cttattccag
    10141 agaagttata taacctttct gtgcctttta actttattca acctattgta gagaatgcat
    10201 ttacacatgg atttagagat attgacacag aaaagcgaat tgaaataatt gcaagattag
    10261 attctcagta tgctattata gagattcata ataatggaac tatcttagat ggaaataata
    10321 tagacaaaat aaaggcaggt attcgtagta ataatggtca tggtttgtct ttaatatata
    10381 caaaattcac atctgcttat ggaaataatt ttgatatgga tataaagtcg agtgataatg
    10441 aaggaactta cataatgatt aaaattccta ttgaaaatta taaggagtat gtatagaact
    10501 tgtgaaaaat taataagttt tataaatgtt aagaaatata ttagatgttt ttatgttgtt
    10561 ttatatatct tactttttta tattattaag ctaaaaggga ggtattctca tgagaagagc
    10621 actaataata gatgatgaag aagctgttct aaaaataata acacatttta tagaaatgaa
    10681 agatatgcca ataaaaattg ttggtaaagc aacatctggt gatgaagcag tagataaaat
    10741 cattggttta gagccagaca tagtttttat agatattcaa atgccaatat acaacgggtt
    10801 agaagtaata gaaaaaacat ctcatttatc taaaaaaata aactttgtag taataactgc
    10861 atttaactat tttgaatatg ctcaaaaagc tttgagatta aatgtaaaag atatactcct
    10921 taaaccattg gatatgaaag aatttactaa gtctgtagaa aaaattattg gatatcaata
    10981 taccaataat gatttattaa atgagatact ggaatatata aatctaaact atagcaatga
    11041 cttaaagtta aatgattgtg ctagattatt tttaaccaat ccatctaata tatctaggat
    11101 ttttaaaagt aatttaaata caacttttat atcatatcta aatcatataa gaatagaaaa
    11161 atctattgag ttacttgaaa atactacaaa aagtataaat gagattgcag aaatagttgg
    11221 atataatagc ctaaataact tttataaaaa ctttaagata gaaaaaggaa tgactccaaa
    11281 agtttataaa ctgaacacta aaaattgaaa ttgataaata aaaagtcaat atagataatt
    11341 gtttgagtgt gtctgaataa gtatacttta attatgataa aaattaaagc gctttaaaca
    11401 tatttaataa tattaaacct tataaaagct gtacctattt atataaaaaa atatatataa
    11461 ataaaaagaa aaagatatac aaaataaaaa aagtgaatta aatactgttt caaaaagaca
    11521 gaattttcat ttaatttact gagagtttat atttcttaaa ggaaggtaac tttataatgg
    11581 aggtaattta atatgtcaga caaagtgaat atgaaaaata ttatctgtat tgcaggcgct
    11641 tatctagcaa cagcaattgg ttctgggttt gctactggtc aagaaattct acaatttttt
    11701 gcctcacaag ggtatatggg tttaattgga gcattaatat caatggtatt gttttcattt
    11761 cttggtgctg aagttataac tagaggtaga gaattaaaac ttaaagaacc aacaagaatt
    11821 tacacttttt attgtgggaa gattttagga aaattttacg aatggtttag tccaatattt
    11881 ttatttggga ttcttatagt aatgatttca ggtgcaggct caactttaac tgaatactat
    11941 ggtataaatc cttacttagg aagagtattg atggctgttg ctactttaat tacagtgtct
    12001 atgggtttag aaaaattagt aagcatactt ggacgtattg gaccagtgat agcagtattt
    12061 acaattgctg ttggattaat aagcttgttt aagcattttg atggtttatc tatggttgga
    12121 gaagctatga aaaatattga agttacaaaa gcatcagaaa gtccaatcgt ttctggtgta
    12181 atatataata cattaaatat tattgtaatg attgtttttt taacaggaat gggagcttct
    12241 attgaaagta aaaaggatgc tttttgggga ggtttattag gtggaatagt gtttatgact
    12301 gctggtatag ttatgtattt ggctatgatt tcagatatag gaaatttgta tatgaaagaa
    12361 ataccatcac tttatttagc agataatata tctcctattg taggtgtatg tttttcagtt
    12421 gcattaatat taggtatcta cacaacagct gttccacttt tatggtctgt aaccaataga
    12481 attgttgaag atgaacatcc taaatttaaa ttggtaacaa cagtaatagc tattttggct
    12541 tgtataggag gttttttacc gtttggtaaa ctagtaaaca ttttatatcc atatacaggt
    12601 tatatggggc ttttaatttt agtctgtatg gtgtataaag caattacatg tagaaaaaaa
    12661 tcaacacaaa gtcaggaata gagccactta ttaatagttt ttatttaaag aaatttttta
    12721 tataatccga atttaaaaag tcaaaatagt taaataaatt gtaaaatatg ataatatatt
    12781 taattaaaat aatatagtaa aatataaata taaactaaaa ttaaatttat aaaaaatatc
    12841 tttataaaga ttattaactt atataatttg tagttaaatt ataaaaaata aaaaaataaa
    12901 attttggaga ggtgttagta tgcatgaact tattttaatc tcagaaaagt ataaagaaga
    12961 tattttagat ttttttacag atattcatat gcaccccgaa ttaagtttta aagaatttag
    13021 gactacaaaa gctataaaag atttattagt gagtttagac atagaaatat tagatttagg
    13081 aatggaaaca ggagttgttg gattattaaa agggaagtat gatggtccca cagtagcact
    13141 aagaggagat attgatgcat tgccaatata tgaagaggtt gatattaagt ataaatccag
    13201 atatgatggt ataatgcatg catgtgggca tgatatacac acgtcttgcc ttgtaggatg
    13261 tgcatatgta ttgtctcata ttagagatag tcttcatgga aatgtaaagt ttatatttca
    13321 accagccgaa gaagtcaata aaggtgcgaa gatgctagtg gaacgaggag taatggaaaa
    13381 tccaaaagtt gatgctatat ttggacttca taatcatcca gatatacctt gtggaaaaat
    13441 aggtgttaag ttaggtgggt taatggcagc agtagacact attaagatag aagtaaatgg
    13501 atttggaggt cacggaggta tacccaacag aacaatagac cctatagtag catcaagtgc
    13561 aattataatg ggtattcaaa ccatagtaag cagaaacata agtccattag aatcagctgt
    13621 tatatcaata ggtactataa atggagggac tgcaaataat gtaatttcag aaaaggtaga
    13681 tatgacaggc acatgtagaa gctttagtaa tgaagtacga aagaaaatat cagaaaattt
    13741 agaaaatata gtttgtgaga tagctcgtgg atatcaagca actgcaaagc tagattattt
    13801 atttgattta ccagctgtta taaatagtaa agagatgtac gcaatagctt gtaagtctgt
    13861 gtgtgatttg tattcagaag atgctatagt tgatccaata ccttctacag gaggggaaga
    13921 tttttctata ttcatggaaa aagccccagg atttttttac tggttagggg taggcaacaa
    13981 agaacaagat tgtatatatc agtggcataa tcctaaattt aaagctgata aaaattctat
    14041 attggtaggt acaaatgtac tatgtcagtc tgttataaat tatatggata aattaaaaaa
    14101 taaaatttaa gatagagcat tctggagata aagtttatga gggggtatta gtttgaggaa
    14161 aattaattca caggaaaaaa acaaagatag tggattattg atgagggcac taaatgttgt
    14221 agagagagca ggaaataaac ttccagaccc agtaaccata tttttattac tatgtataat
    14281 tgttgtaata ttgtcagcag ttatatctaa tttaggagta gaagaaatac atccttctac
    14341 aaaagaagtt gtaaaggtag taaatctact agagaaagag cagattcaat catatttagg
    14401 tagcatagtt acaaactttc agtcatttgc tcctttagga ttagtactgg ttacaatgct
    14461 tggagcaggt gttgctgaga aatctggatt tatggaagtg ttaatgaaaa agggtataag
    14521 taaagttcca caaaaacttg taactgtagc aatcgtattt gcaggtatgt tgtcacatac
    14581 agcagcagat gtaggattta taatattacc accacttgca gcacttgtgt ttttaggaat
    14641 tggaagacat cctcttgtag gtatgtttgc agcttttgca ggtgttgcag ggggatttgc
    14701 agcaaatgtt atgcttagta ctacagatgt attgttagct ggatttacaa taccagcagc
    14761 tcaaatgatg gacccatctt accaaggcaa tccagcaatg aatctttatt ttgcttttat
    14821 atcagcgata gttttaactt ttgcaggagc atttgtaact gaaaaattta tagctcctcg
    14881 ttttacaaaa tataatgatt caacaaatga taaggatata caggaattga gtgaacttga
    14941 aaataagggt ataaaatacg ctttattatc attgttagtt gtagtagtag tgatagttgc
    15001 cttatgtata ggtgacaatg catttatgaa agatccagag actggttcta tactatctag
    15061 taatgcacct ttaatgaaag gtatagttcc tataatcact attatatttt taactccagg
    15121 attagtgtat ggaaaagtaa gtaagaagat aaaaagtgac aaagatttag tatctatgat
    15181 gggtagtagt atgagtgata tgggtggata tatagtccta gcatttatag catcccagtt
    15241 tattaattta tttaatctta gtaacttagg aacaatttta tcaataacag gagcaaaatt
    15301 actagcagag tcgggaatac ctagctatgg attgataata ggatttatat tgctatctgg
    15361 atttatcaat ttatttgtag gaagtgcttc tgcaaaatgg gcgattttag cacctatatt
    15421 tgttcccatg tttatgctac tagactttaa tccagcatta actcaaatag cttatcgtat
    15481 tggagatgct tcaacaaatc caataagtcc actttttccg tattttccag ttatattggc
    15541 ctttgccaga agatatgata aagatatagg tataggtact gtcatatcga atatgattcc
    15601 atactcagtg gtatttacat taatagaaat aataatattg ttactattca tgggtattgg
    15661 cataccatta ggacctggtg gaggaatatc ttacgtttta tagaccgtta taataaattt
    15721 atcaatgaag attaaactaa taaaatatat tctttaatta agaggaggat tattatgtta
    15781 aaaacaacaa aaatttcaga cccagagtta tacaagattg tagctgatga gttggttaga
    15841 caagaacaca atattgagat gatagcttct gaaagtacag caccaacaga agtacttgaa
    15901 ttaagtggat gtgtatttac aaataaaaca gaagaaggct atccaggggc tcgttttcaa
    15961 gcaggttctg aagaagcaga taaacttgaa accctagcaa taaaacgtgc aaaagaagta
    16021 tttggagcag aacatgtaaa cgtacaaccg tattcaggtt ctacagctaa ctattgtgta
    16081 tattcatcaa tattaaaacc aaatgacact gttttaagta tgcgtcttga ccaaggtgga
    16141 cacttgacac atggtagcgc agttaatttt ttacatgaca tttataaata tgaattttat
    16201 ggggtagacc caaatactgg acgtatagat tatgatgctc ttgaagcaaa ggcaaaagaa
    16261 tgtagaccaa aacttataat tgcaggggca agttcatatc caagattaat agattatgaa
    16321 agaatatcaa aggttgcaaa agaagtagga gcttacttta tggtagatat ggcacatgtt
    16381 gcaggattag tcgcagcgaa agtaatacct agtcctgtac cttatgcaga ttttgtttct
    16441 tcatctacga caaagacttt ctgtggacca agaagtggta ttgtactttg taaagcagaa
    16501 catgcaaaga aactcgataa aggtgttttt ccaggaacat taggttctat acatcttaat
    16561 acagttgcag caaaagcctt ttcactatta tatttaagta cagataaatt taaaaagata
    16621 atggagcaag ttgtagtaaa tgctcagact ctagcttctg aactgatttc acatggtttt
    16681 agtattgtaa gtggaggtac tgataatcat atagtaatgg ttgacttaag aagtaagaat
    16741 cttacaggaa aacaatttga aaaagctctt gaatatgtag gaataacagt taataaaaat
    16801 gttatacctg acgacccaca atcaccattt gtaactagtg gtgttcgtat aggtcttaca
    16861 tcaatatctc aaagaggtct gaaagaaaaa gaagttatac agatagcagg gataatgaat
    16921 aaggtagcag aaaatattga taataaagaa gtattagatg aatgtaaagc agaggcacaa
    16981 gaacttatat ctaagtttcc attatatcca gaagggtatt ttgaagatta aatattttga
    17041 tttgtatagc tcacaattta taaagcctgt aacataaaat ttaataatat ataaccacta
    17101 taaggtgttg attaatcaac accttatttt ttttgaaaag taatatacaa gctatcataa
    17161 gcatataatt ccttatagat aattaacaag ggggataaag ttatgaggta ttataataaa
    17221 cctaccaaag aagtattaaa atacttaaaa acaaatccag aaattggact tgatgataat
    17281 gaggtagaag aaaggaagct tagatatgga ttaaatgaat ttactataaa agaaggtaga
    17341 acattttggg acgagttggg agaaagtttg acagaaccta tgatattaat tctcatagga
    17401 gcagcagtaa taagttcttt tgtgggagaa ttacatgacg cattaggtat tttaggggct
    17461 atttttattg gaatttctat aggcataata actgaaggaa aatctaaaaa agcagctcat
    17521 gccctgtcaa aattaacaga aaatatagaa gtaaaggtgc ttagaaatgg gaagataatt
    17581 aaaatatcaa aaaatgattt agtaccagga gatattgtat atatagaaac tggggatatg
    17641 ataccagcag atggaagact tatacagtct ataaatttga aacttagaga agatatgcta
    17701 acaggagaat ctgatgatgt agctaagaat gctgatgctg tattagatat ggaagtagtt
    17761 tattctaaaa cagaaataat cgaacaggat gcaatacctg caaagcaagt caatatggta
    17821 tttggaggga ctcttgtagc atatggtagg ggcataatgg ttgtcactca tactggtgat
    17881 aagacagaga tgggtaagat agcacagaat ttatcaaatg aagaccagca aactccactt
    17941 caaattaaat tagggaagtt gggtgctaaa atagcaggaa tatcaggcat aatagctact
    18001 ttattgtgta tgtttatgat tatacagatg cagagaaaag gtatgttaat tttagataca
    18061 agcagtatat tatctttctt acaatcatta gaacctgcta aaaatgcata tatggtttgt
    18121 attgcattaa tagttgcaac tgttccagaa ggtttgccaa ctatgataaa tattacctta
    18181 gctataacaa tgcaaaagat ggcaaagata aatgcattag taacaaagaa agaagcctgt
    18241 gaaactatag gttctgtttc tgtaatatgt tccgacaaaa cagggacttt aacacaaaac
    18301 aaaatgatgg tagaggtagc atatgttgat ggtaaatata taagtggagg agagtatcaa
    18361 agtaatagct attttgaaca aaactgtata gtaaattcaa ctgcagatat agaaaaagaa
    18421 gataattcat ttaaatatat aggaagcgca acagagtgtg cattactttt atatcataat
    18481 gataagaact ataatgaaat gagaaaacaa acgtatttga tatcacaaat accatttagt
    18541 tcagaagaaa agaagatgtc aacattaatt cgtcaagagg attcagatat attactttca
    18601 aaaggtgctc cagaagtatt attaaaaaaa tgttcttatg tacaacaagg aaaaaatatt
    18661 gttccaataa ctccaaaagt agaaaaaagt attttagatg agattaagaa gttacaaatt
    18721 aaatctatga gaacattagg atttgcttat aaaaaaatga gtaattctaa aacagaagtt
    18781 gcaatgactt cagaaggtga attgaattta ataggaaatt caagaagtta tatgaaggaa
    18841 gataatttag tttttagtgg atttgtagga atagtggacc cattgagaga aggggtcaaa
    18901 gattccattg ataaagcatt taatgcagga gtagatgtaa aaatgcttac aggagataat
    18961 ataaatacag caacagctat tggaaatgaa ttaggattat taaatgatgg gaaaaaggca
    19021 gttgaagcaa catatataga tgtattgaca gataaagaat tgagagaaga aatcaaagga
    19081 atatctattg ttgcaagaag taaaccagat acaaaaatga gaatagtttc agcacttcaa
    19141 aaaagtggcg aagttgtagc tgtaacaggt gatggtataa atgatgcccc agcacttagt
    19201 caagcagatg ttggaattgc aatgggtatt tcaggaacag aagtatctaa aaatgcagca
    19261 gatataatat tgactgatga tagctttagt actatagtag agggaataaa atggggaaga
    19321 ggtatatatg agaactttca aagatttata cagtttcagc ttactgtaaa tatagtagca
    19381 tttataattg ctataatatc tcaacttact ggtaaggata tgccatttac tacaatacaa
    19441 ttattatggg taaatataat aatggatggt cctccagccc tagcattagg attagaacct
    19501 gttagagatt atgtattgaa aagaaaacca ataaatagac attctggaat aatagctagg
    19561 tctatgtttg taaatattat tataaatgca attcttataa ttacaattgt atttactcaa
    19621 tcagctttta atatattggg agctacatca gaagaacaag gtactgttat attttcttta
    19681 tttgcattca gtgccctttt taatgcatta aattgtagag agtttggttt aaatagtaca
    19741 ataccaaatt tctttaaaaa taaactggca ttgcaaataa tagtagttac agggataatt
    19801 caaataatat ttacacaagt gttccagagt ttctttaatt ctgtttcttt ggactttgat
    19861 atgtggataa agataatact atttgcatcc actatcctat tgtcaaatga atttgtaaag
    19921 ctaattctta gaacaatgaa aaatagtaga actatgaact ttaatagtaa aaattaattc
    19981 ttatgaaacc gtaaagtact atatattgta actttgcggt ttttgtttgt ttaatgttgt
    20041 tttatattac cattttcata tttaattttt tattaatctg aacacaatct tccattgtaa
    20101 ttgcttcatc tgtgtcaaca cattcaaagt caatatctaa tttttgtaat attttatatg
    20161 cctctttttg caaaggagta tcaaatttaa taggtgattt tgtgtaaata tcactgataa
    20221 aaaacatgat ttatttcatt cctttccatt taaaatctta tagttatagt gtatcataag
    20281 gttataaaat atagataaat atagtataaa tttaaaatat gtacagacaa aacaaaaaaa
    20341 tcatattcta agtaatactt gggaaaaatg atattatca
//
LOCUS       DAELTA010000067        20148 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.067, whole genome shotgun sequence.
ACCESSION   DAELTA010000067 DAELTA010000000
VERSION     DAELTA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.067"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..2487)
                     /locus_tag="KPG13_001990"
     CDS             complement(<1..2487)
                     /locus_tag="KPG13_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0985722.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKT"
     gene            complement(2501..3043)
                     /locus_tag="KPG13_001991"
     CDS             complement(2501..3043)
                     /locus_tag="KPG13_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985723.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3287..4801
                     /gene="glpK"
                     /locus_tag="KPG13_001992"
     CDS             3287..4801
                     /gene="glpK"
                     /locus_tag="KPG13_001992"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG0985724.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGVAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(4915..5544)
                     /locus_tag="KPG13_001993"
     CDS             complement(4915..5544)
                     /locus_tag="KPG13_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0985725.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            6132..6923
                     /locus_tag="KPG13_001994"
     CDS             6132..6923
                     /locus_tag="KPG13_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG0985726.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            6952..8172
                     /locus_tag="KPG13_001995"
     CDS             6952..8172
                     /locus_tag="KPG13_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG0985727.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            8192..8887
                     /gene="pxpB"
                     /locus_tag="KPG13_001996"
     CDS             8192..8887
                     /gene="pxpB"
                     /locus_tag="KPG13_001996"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG0985728.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            8917..9903
                     /locus_tag="KPG13_001997"
     CDS             8917..9903
                     /locus_tag="KPG13_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG0985729.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(10117..10683)
                     /locus_tag="KPG13_001998"
     CDS             complement(10117..10683)
                     /locus_tag="KPG13_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985730.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            11035..12102
                     /locus_tag="KPG13_001999"
     CDS             11035..12102
                     /locus_tag="KPG13_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG0985731.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            12121..13281
                     /locus_tag="KPG13_002000"
     CDS             12121..13281
                     /locus_tag="KPG13_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0985732.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            13294..15390
                     /locus_tag="KPG13_002001"
     CDS             13294..15390
                     /locus_tag="KPG13_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG0985733.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(15549..15893)
                     /locus_tag="KPG13_002002"
     CDS             complement(15549..15893)
                     /locus_tag="KPG13_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG0985734.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            16185..17075
                     /locus_tag="KPG13_002003"
     CDS             16185..17075
                     /locus_tag="KPG13_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0985735.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            17293..18579
                     /locus_tag="KPG13_002004"
     CDS             17293..18579
                     /locus_tag="KPG13_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0985736.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(18668..19111)
                     /locus_tag="KPG13_002005"
     CDS             complement(18668..19111)
                     /locus_tag="KPG13_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG0985737.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(19135..19929)
                     /locus_tag="KPG13_002006"
     CDS             complement(19135..19929)
                     /locus_tag="KPG13_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG0985738.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         7475 a         2435 c         3398 g         6840 t
ORIGIN      
        1 agttttattt tctttataga acaataatat tataaatgct agtagattta cacagaatac
       61 tgttatatac atctgtttaa atcctgagtt tagagtagat tgtagtgtgt tttctatttt
      121 tgttctatta ttttctatgg aatttatata atccaatttt gatttatcaa aagcctttgg
      181 tatttcagtt tttacctctt gtgtctttgc taaaactgct tggagtgatt ttttttgctg
      241 ttgcattgta tcaaggcttt tttccatgtc tttcttttgg cttatagaat tatttaactt
      301 gctttgtagc tctttctttt gagattccat tgttcttagt tttttactta attcgttctt
      361 ttgagtttta tttttttgta actttgtatt taagtcatct cttgtctttt ttagttgctg
      421 tatttgagca tttaaacttt ctttttgttc atttacattt ggtttattct cgctggaatt
      481 gttattctta ttttctgtag aatcattctt agtgtcgtct gattcagaat tactggtatt
      541 actctggttt tgttcatttg agttattttc gttatcagta ggagtttttt ctgtagtagg
      601 tattttatta taagcagttg taagttcctt gatggcctca tcttgttttt caagaccttg
      661 ttccatacct tttatacctt tatcaacacc agcaatacct ttttttacac catctattcc
      721 ttgattaata ccagctctag cttttaccat attgtcaata ccagtttgaa ctcctttaat
      781 accactattt agtttatttt caccatcttc aataccgttt attccacttt gtgttccatc
      841 tattcctttt tgtacatttt tctgtatttt agtaattata gatggaactt tttcatcaaa
      901 cattgttgtt gatatttctt ttgttatatt ggtaatgttg gttgtatctg ctgactgaac
      961 tttattaagt aaggattttg gaagtttgcc acctgtcatg tccatattta tatttgaagt
     1021 atcattcata tttgggatag agacattttt taaatttttt gccatttcag ggtctgattt
     1081 taagtcatct atcatgtttt caagttcttc aagttgaact attttaggag ttgagggctt
     1141 tccaactaag tccataatat tatcttgcac agataatcct gcatgtgcta taaatccaat
     1201 cattatagct ggagaaattg aagtaccaat tgagcgtatc aaggataatg ttgcaagtgc
     1261 tgaattagat tcttctaatc ttgtatttga taacatcata tagtttagag gagttcccat
     1321 agtaaatccc attcctaatc ccataaatat taaacttaca catacactaa atacagtatt
     1381 ggtttttaga gcaattaaaa ctaggtaaag agagcctatc atagatacag aaaatcctag
     1441 taatagtatt ttttttgccc caaacttgtc aataagtctt ccgcttaaag gagcagcaat
     1501 acctgcaaat aatcccaata tagctacaaa atatccccca gaaccagcat ttatctttaa
     1561 tgcattttca gcatactgtg gaacgaatac catacccatc attccaatac caacaattaa
     1621 agataaaata agtactatca atattctcgg atttaaaaaa tattcaaagc ttaaaatagg
     1681 gtctttagct ctattttcaa taaatatgaa aataggtata agtacaataa atgtcaataa
     1741 gtatggataa actgatatat cttgtataga atttttaaaa ttaaaaaagt ctatattcat
     1801 aagaccatat aatagtgata cgattataca tatcaacatc aatgtaccaa gcttatctat
     1861 tttttcttta gattcagact tattgttttt aatacagaaa actccaccaa taagtatgat
     1921 taaacaaatt ggaagattta caaaaaatag ccacttccaa ttttgagttc caaagatact
     1981 tagaattgtg ctaccaatgc ttgaacctaa aatattggca ataccatatg ttgccccaac
     2041 taaacctaaa gccatgcctc ttttattttc tgggaaggta gttccaaatt ctgctgtagc
     2101 aattggcatg attccaccac caccaattgc ttgaataaca cgaccaatta ataaaatata
     2161 gaaattggaa aatagtgatg ataatccaca tattaatgaa ccactaccaa atagaaatat
     2221 actaactaga tacacatatt ttcttcctat tttatctgct aattttccag agataggaat
     2281 tacagaagcg tatgttaatg tgtatatggt aatcatccaa attcccgttt tttcatttac
     2341 tccaaggcta ttttgtataa tcgttcttgc tggtgatact attccagtat caatagctcc
     2401 cataaatata cctaatagat aaagcgtaag tactaaaaat atttttggat tactgtcctt
     2461 gttcttaata ctagtattat gattcaaggt aaacaccacc ttaatgtatt aatttttcaa
     2521 tatagggttt taaatcttca aattctatat gaagatatgt tgaatatgaa aaaaacattt
     2581 ttattatgat attgcatact aatttgtcat tattaaaaaa actttctttt tctaaagctt
     2641 catatacttt atttgaaagc tgttcatgaa agtcgtcttt gtgttttttc atttcaaaaa
     2701 atctctctgt ttcaaaagtg tttaaaaagt cattgtattg gatattatga actttgttta
     2761 taaaatttct tatttcagta aaaataatgt ttagtttttt tatgtaatct tcagtgtttt
     2821 tattggagta ttcaattcgc cttatcaata aatcccattc agattttgtg atatattcta
     2881 ttatttcttg ttttgaatta aagtaattat acactgtacc tgcacttata ccacaccttt
     2941 gagctatttt ccttatgcta atattcgagt aatcttcttc taaaatcata tcccgagaca
     3001 cttttaatat atcttctttt acattttcta aaatctttgg catattacca cctcttttat
     3061 taaaattatt atgaacactg tttattatac tcctttaaaa tttattaatc aatatgtaag
     3121 ctataatgta taatattatt atatagatat gatagaaaat aagtgtcttc ttactaaata
     3181 tctattatat taaatttgta cttaagtatg attatttatt gaaatatgtg atattaaatt
     3241 tttcttttta ataaattaaa tattatatga taaggagaat atggtgatgg aaaagaagta
     3301 tgtaatggca ttagaccaag gaacaaccag ttcaagagct atactattta ataaaaaagg
     3361 cgaaatagtg aaaattgcac aaaaagagtt caatcaaatt tatccaaaag ctggatgggt
     3421 agagcatgac cctatggaaa tatgggggtc acaaagtggg gtaatgagag aagttataga
     3481 aacagcaggt ataagaccag aagaaattgc ttcaataggg ataactaatc aaagagagac
     3541 tacggttgtt tggagtaggt atactggtaa acctatttat aacgcaatag tatggcagtg
     3601 taggagaacg tcagaaatat gtgatgaact taaaaataaa ggattggaag aatcaataaa
     3661 agaaaaaaca ggtcttttaa tagatgcata cttttcagca acaaaagtaa aatggatatt
     3721 agataatgta gaaggagcta gggaaaaagc agaacaagga gagttattat ttggaactat
     3781 agatacatgg ttaatatgga atttaactcg tggaaaagta catgtaacag attactcaaa
     3841 tgcatctaga acaatgatgt acaatataaa tgatttagaa tgggatgagg atatattacg
     3901 agaattggac ataccaataa gcatgttgcc attagtaaaa ccttctagct atgtttatgg
     3961 tcatacagat gagcgcatgt tatcaggagc taaaatacct attgcgggat gtgctggaga
     4021 ccaacaagca gcattatttg gtcaaaattg tgttgaagaa ggtactgcaa aaaatactta
     4081 tggaacgggt tgtttcttgc ttatgaatac aggcagtaac atagttaaat ctaagcatgg
     4141 gttacttaca acaattgctt ggggagttga tggaaaagta acatatgcat tagaaggaag
     4201 tatatttatt ggaggagcat ctgttcaatg gcttagagat gaattaaaga taattgaatc
     4261 tgcaaaagat agtgaaatgt atgcaaatag ggtagaggat actaatggtg tatatgtagt
     4321 gccagcattt acaggtcttg gagctccata ttgggatatg tatgctaggg gttcaatttt
     4381 aggacttaca agaggagcaa aaaaagaaca tatagtaaga gccacattgg aatcaattgc
     4441 ttatcagaca aaggatgttt tagaagcaat gcaaaacgat tcaaaattaa aactaaaatc
     4501 attaaaagtt gatggtggag caagtaataa taatttttta atgcaatttc aatcagatat
     4561 attaaatgta gatatagata gacccaaaat agtagaaact acagctttag gagttgctta
     4621 tttagcaggg ttgtctgttg gattctatat tggaagaaat gaaattactt caaagtggtc
     4681 agtagaaaaa gagtttaatc caaatatgag tgaagagaag agatgtaaat tatataaagg
     4741 atggaaaaaa gctgtatcga gagctttaag ctgggaaaaa gaagatgaat tggatattta
     4801 aaaattaatc aaagttaaat ttatttgaat ttaaaaagag ctttatgtaa aatatgaagc
     4861 tctttttttg ttgttaatct tgcattttta tataatcaca ctaaattata taatttattt
     4921 ttcgttttca tataaaaaca ttggtataga attgtaaatg tgttggtcac caactactaa
     4981 gagaatgtct ccttctaaaa attcaatata tggtccagga gatattataa gttcttcacc
     5041 tcttttaatt gcaactattg tagcacctgt attttgccaa aatttagttt gtgcagctgt
     5101 ttgaccaata tatttgcaat tagaatttat ggtgaactga acgggcacta aaggattgat
     5161 tatttcaagt ctgttagaat agtctaaaat tttatttata gtatctaata tttcattttc
     5221 taaattagat ttagtatcta ttaaattata aagtgttgat ttatatgatg atatatttgt
     5281 aatatctcta tattttttta taaaagaaga agctttgtct ggagatagta caataacacc
     5341 tttacccttt atgcttttaa caacgccaat gtcctctaaa attttgacag ctcttcttat
     5401 cgtttctgga gatacattgt atttaccagc caaaacagag cgtccaaata gtactgaatc
     5461 ctcttgtatt tcacccgtgt atattttatt tgcaatatcc aacgctattt tttggtatat
     5521 tggcatcgta tagtttttat ccattgtttt tcccccaata caaaatttat gttaaaaaaa
     5581 tatataatct taacaatact attgtataat ttataggttt atatgtctac aatatgtaca
     5641 aatattgtgc atgttttaaa ataatatttt aacatgcatt tagtatacat gattttatta
     5701 tacttaaaac tatataaaga attaattata aaaatataaa gatattttac ttacaaagaa
     5761 tgataattat atagatgaaa gaaacaaatg aatgaagaaa aatgattctg tcttgcatat
     5821 aaagtgatta aaaatgaaaa atactttaaa aatgaaggtt aaagccctta ttttaagtga
     5881 aaataattaa ttattaacta aaaaatggta aaaagatttt ttgaaaaaga aatacaaatg
     5941 aatgttcaat taaaataatt gcaaaaatat tccttaaatg gtaaaattat attaattaaa
     6001 tgcaatagaa tagttgcaaa aataaaattg aatacaagtg ataaaatatt tcatgcaata
     6061 tataaaataa acaaaattta aaaattaaaa ctacttattc tatcaaaatg ttagcgagga
     6121 ggggtcataa attgtataaa gtagatttaa atagtgattt aggagaaagt tttggaacgt
     6181 ataagattgg tttagatgaa gaagttttaa aatatatttc atcagccaat atagcttgtg
     6241 ggtttcatgc aggtgaccct agtcatatgg aaaaaacagt acagctagct aaaaaaaatg
     6301 gagtgaaaat aggcgcacat ccaggtttcc tagatttaat aggatttgga agacgtgaga
     6361 tgaaaataac aaagcaggaa gctaaagatt atacaaaata tcaactgggt gctttaatgg
     6421 catttgcaag ttcaaatgga tgtaatatac aacatgtaaa accacatgga gcattataca
     6481 atatggcagc taaagataaa gagttagcta tggggatttg tgaagctata tatgaagtcg
     6541 ataaagatat tatactgttg gggctatata atagtgaaat gataaattct gctaaagaaa
     6601 ttggacttag gtttgcaaat gaagtatttg cagatagagc ttatgataat aatggttttt
     6661 tggttccaag aaatgtagaa ggagcagtta tacatgatac aaaacatgca atagataggg
     6721 ttgttaggat ggtaaaagaa ggaactgtag aaactttaac aggtgaagtt atacatatca
     6781 aagcagattc tatatgtgta catggggaca atccaaaagc aatagagttt gtcaaagaaa
     6841 taagaaagcg ttttgaatta gaaggtatag aagtttgtcc attagaaaac atagaagttt
     6901 gttcgtcaga gaacatagta taagactgat aacatgaggg ggagataaaa tatgagtaca
     6961 aaaaatatta cagataaaac taaaaataaa aaagatgtcg gagcacttat aggggctgca
     7021 tttataatgg caacatcagc aattggacca gggtttttaa ctcagactgc acaatttacc
     7081 caagattttg gaccgaattt ttcatttgta attttaatta caacaatcct ttttataggg
     7141 gctcaagtaa atgtatggag agtaattggt gtatcaggac ttagaggtca ggatatagca
     7201 aataaaataa tacctggatt aggctattta gtagcttttt tagtagctct tggaggatta
     7261 gcttttaata taggaaatgt tggtggagct gcacttggta tgaatgtaat gtttaatatg
     7321 aatatgacat taggaactgt attaagtgga ttaatagcta tatttgtatt tatgtcaaaa
     7381 aattcaaatt ctctagtcga taagataact aaatttttag cattaggaat gataattata
     7441 gttggatatg tagcaatatc aaatcatcct cctgttggag aggcagtatc aagaatggta
     7501 aaaccagaaa atccaaaagg actgatattc ccaataataa ctttacttgg aggaagtgtt
     7561 ggaggttata taacttttgc tggaggacat agattaatag acggtggaat aacaggagaa
     7621 gaaaatataa aagaaataac taaatcttct cttttgggta tattagttgc cacaatgatg
     7681 agagttcttt tatttttagc aatactggca gtggtttcaa aaggtcttca acttgaccca
     7741 gaaaatccag cagcttcagc ttttaaattt agtgcaggag caataggata taaattcttt
     7801 ggacttgtat tatggtctgc tgcaattaca tcagttatag gagctgctta tacatcagtt
     7861 tctttcttaa agacactaaa tccttttata gataaatatg aaaaatattt tataatagca
     7921 tttatagcaa tatcaacact tataatggca tttataggaa aaccagctac tttattaatt
     7981 ttagcaggag ctttaaatgg attgatactt ccaatcacat taggaattat gcttatagca
     8041 tctaaaagaa aagatattgt tggagattac aagcatccta catggctttt aatatttgga
     8101 ttaatagtag ttttaatatc tgcatataca ggaataactt ctcttagcag cctaggtgct
     8161 ttatttgctt aatttagaag aggtgacatt tatggaaact agatacttga tatcagggga
     8221 taaggcagtg gttgctgaat ttggaaatga gatatcagaa gatataaaca aaaaagtaat
     8281 ttcatttatg agagcaatag aaatatcgaa tcttaaaggt attgtaacag aaatggtacc
     8341 aacatatagg tcacttatga taagttataa tccattagaa atagattttg atagtttgat
     8401 tgagaattta aaaaaaatag aagataattt ggaaagtata gtattaccaa aacctaaaat
     8461 acatgagatt ccagtatgtt atgatgaggt ttttggtata gatattaaaa acgttgcaag
     8521 ctacaataat ctcactgtag atgaagttat aaaaatacac acaagcagag agtacttgat
     8581 atatatgcta gggtttactc ctggatttcc atatctaggt ggtatggatg aaagaatagc
     8641 tacacctaga ttagaagtgc caagaactaa aatttatggt gggagtgttg gaatagcagg
     8701 ttcccagact ggtgtatatc ctattgatag tcctggtgga tggcaaataa ttggtaggac
     8761 acctttaaaa ttatatgatg aaaatagaga agaacagatt ttgcttagag ctggagactt
     8821 tataaaattt gttccaataa cattagatga atttatagaa atcgaaaaaa ataacttaag
     8881 tatttaaata tcgttatagt acagggagtg gtatatatgg gattcaaagt tgaattagga
     8941 ggtttccaaa cattaataca agacagaggt agagttggct atggtcaata tggtgtatct
     9001 ggatgtggag caatggatga gtatgcacat agagtaggaa atatattagt aggaaattct
     9061 gaagatgagg cttctttaga agttttaatg ttaggtccta caattacctt tgatgaatat
     9121 acgcaaatag cagtaactgg gggagattta ggggctaaaa taaatggaaa agaaattcaa
     9181 aactggaggt catatcaaat aaatccaggt gatgtattat catttagagg tgtgaaatca
     9241 ggtactagag cctatgtgtc aatagctggt ggaatagatg ttccacttgc aatgggaagt
     9301 aagtcaactt atacaagagc taaaattggt ggatttgaag gtagagcact taaaaaaggc
     9361 gattatataa atacttttat tcaggaaaaa gattttacaa taaataaaaa attaagttca
     9421 aaatatattc caacttatag ttcagaaatc gtactaagaa ttgtaaaagg tcctcaattt
     9481 gatgcttttt ctaacggtga agtagagaaa tttttatcaa ataaatacaa agttacgaat
     9541 gaaatagata gaatgggttg tagactagat ggagagagta taaagcacct taatggagca
     9601 gatattatat ctgagggtat ttcttatgga gctatacaag tacctggtca tggaaaacct
     9661 attataatgt tatcagatag acagactagt ggtggctata ctaagatagg gaacgttata
     9721 agtgtagatt tgtataagtt agcacaagca aagccaaatg atgtagtaaa atttgaactt
     9781 gtagatatat atgaagcaca tagactattg agagagcaag aagataagat tcaagatatc
     9841 tacaagagta tgaaaaatat aagagttgta aaggcaaaag tattaaatga tatagcagtt
     9901 taattttaac aaatacaatt tttattatag tttataatat aacttacaaa tagctattta
     9961 ctatttacca taattaagat ggtttagatt agtcaaatag ctattttttt agtttgagta
    10021 aatattttaa atttaaataa atacttcaat tttaaaataa aagcaatatt taaatctatt
    10081 atattatcta caaaaagtag gtgtgtcata ttgagtttat ttagatttta ttggccaaat
    10141 tggaggtgat ataactccat atccacgagc tattgtatct ttacttggat tgatgatttt
    10201 atgtggtaca ttagctttta taaatgttga gtcacctgga tttaatctaa ctaccttttc
    10261 acccatacaa atatcaacca caccactcgt taccaccata atttcttctg atgcatgata
    10321 tatataatct ttggcatcct cactttctgg tttaagttca aactctacaa ataaaattga
    10381 aggtgtaaac tcagttgtag gcattggaga tactatctcg taaaacattt cactctttgg
    10441 aaatttcatc ataactctat cttcttttct aatgactaaa tcatcagtat tattgtcatc
    10501 cataaacata tataaaggag tgtctaaaac ttcagcgatt tttctcaatg aagacaagga
    10561 tggttcaata gcatctcttt ctatttgaga gatataactt atagataaat ctgtcaattt
    10621 ggaaacatca cttaatgttt ttttcttgct ttttcttata tttctcatct tttcacctag
    10681 cataagaacc tccaaataat aaaattaata taaaatattc gcttaaaaca aagtaattat
    10741 agcacaacat agaagtagtg gcaataagaa tacatagtag attttgacaa ttaaaattta
    10801 attaaatcca aataaaagtc tatgaaaatg aattattgtt aaattaatac aaagaaaata
    10861 ataataaaaa aatttaatag aagagtatta attgaattaa gattatcaca ttatattttg
    10921 gaaaagagta aattttcagt aaattatctt gacatatata ttgaagtaat aatataattt
    10981 aaagtataaa attttaaaaa ttgaattata acttcaaaaa ataaggaggg taatatgaag
    11041 ataacagata tcaaatttga aaagcttaga attaaattaa aaaaacctgt agtagtttca
    11101 tttggagtaa ttgaatatgg agaaagtata attttaaaaa ttgaaactga tgaagggtat
    11161 tgtggatttg gcgaagcagc gcctcttgca gcagttacag gtgaagtttt ggataatgtt
    11221 ctttcagtat tgcttatgtt taaaaaagag ttaataggca aagacccact agatattgaa
    11281 accatacata caatcatgga tggaattatt ataggtaata cttctgcaaa agcagctatt
    11341 gacatagctt tatatgatat taaaggaaaa attatgaatg caccccttta taaagttctt
    11401 ggaggatttg atagtaaagt ccaaacggat attactataa gtatagataa gccagaaaaa
    11461 atggcagtag aagctttaga aagagtaaaa gaaggtttta ggatattgaa gcttaaagca
    11521 ggtataaatc ctgaagacga tattgaagct gtaaagttaa ttagagaagc cgttggagag
    11581 agtataagaa ttagaattga tgcaaatcaa ggatggaacg taaatagcag tataaatact
    11641 atcaaaaagt tagaagaatt taatgttgat gcaattgaac aagcacttcc acattgggat
    11701 ttagatggta ctgcttacat aagaaataaa agtaatacaa aaattatgat agatgaatca
    11761 ttacattcac caatagatgc aattaaggct ataaaaaaga atgcagttga tacatttaat
    11821 attaagctga tgaaatcaag tggaatatat cctgctataa aaatcaacaa catagctgag
    11881 gcaagtggag taaattgtat gttaggatgt atgctggaaa cgagaattgg tataacagca
    11941 gcagcaaatt taatagcatc taaaaagaac ataacagaag ctgaccttga tagttttatg
    12001 ttttgtgaag aattagaagg tatatctgga ggatttgtaa tggatggaga tattatgaat
    12061 cttgtcaata aaccaggttt aggaatagaa gtaaacttat gataaatttg gaggttaaat
    12121 atgaatacac ttttttcaaa tccaggtagt atacttgctg taatgacatc aatgattgca
    12181 ttgggttttt acttacaaag atacaaagcc attaaaagct taggaccagc acttacaata
    12241 atcataatgg gaataatact ttcaaactta aaagtagttc cagtaagtac tgagttatat
    12301 ggaactattt caacatatgc tattccagtt tctatgacta taatgttgat gagtgttgac
    12361 ttaaaagaaa tgacaaaatt atcaagagaa cctttaattg caatttttgt agctgtatta
    12421 actgtaagta tcatggcatt tttatttgga ttagtatttg cagaaaaaat atcagaaggt
    12481 tggaaggttg ctggaatgtt tgtaggtaca tatactggtg gaagtgctaa tcttacagct
    12541 attggaacag gtcttaatgt aagtagacaa actcttgctg cagcaaatgc agcagactat
    12601 gtaattggag taccaacatt gatatttatg tttgctctac cagcaatatt aaaaaattca
    12661 aagaagttta aaaaactttg gccatatcat gtagaagaat cagaattaga ggattgtcaa
    12721 aatgaagaat ttatggagtc aaaagaatgg agtattaaag atatagcttg gatgttggct
    12781 ataggttttg ttgttacaga agttgcaaca atacttgctg gatatttcaa ttcgagtttt
    12841 agtagtgcag caagaatact tttagttaca acaatttcaa ttataattgc tcaattaaag
    12901 ccagtaaaga aactaaaggg aaatttagat ttaggattat ttgttgcatt attcttttta
    12961 tgtacaatag gtttttcagt agatataaaa gaatttttag gttcaacttt tactataaca
    13021 ctttactgtt ttagcataat ttttgcttca tttgttttcc atttaggcat aactagactt
    13081 ttaaagataa agtatcaata tgtaatactt tctatagtag gagcaattgc tgatggtcct
    13141 acttcagctt tagtagctgc ttctgcaaag tggaattcgc ttgtaagtgt agcagtcgta
    13201 atgggagtaa ttggtggagt attaggaaat tatgcaggta tatcagtggc atatgcaata
    13261 aagatgtctc taggattata ggggggagtt taattgataa aatttaaatt atacatatct
    13321 ggactataca gtggaaatgt aatctttgat ggagatttat taattgaaaa gttgaatcct
    13381 tttacaaata aaatagaaag tttaaaacca ataagcaagg aagaaaatac atactattta
    13441 aatcttacaa aaatagattt aaagtcttta tttaataatt ttgatgtata tactaaaagc
    13501 ttagtaaaca cagataaaga cacagttata aataacttag gagaaacgca ttctaaactt
    13561 aatgaatata tatgggttca aagaaacaaa aagttcccac tagatataat tattgtagat
    13621 aataaaatag taggttttat ctgtttatcc agagaaactt gtactatatt aattatggat
    13681 ggatatgaag agtatactgt attaaaagaa tgggaaaaaa ctcataaaaa tgaagagata
    13741 tattctataa gatttggtgg aaattatatg attgatatga aagatggtat aaaactttct
    13801 acagatgtat atctccctga ttttgtagat tctaccaaaa aagcaccgac aattcttatg
    13861 agaactccat atggaaaaga aaatgataaa gaaatatatt ataaatatgt acaaagagga
    13921 tatgctgttg taatacaaga tgttagggga agaaatgaat cagaaggtaa gtgggaacct
    13981 atgattcatg agagggaaga tggagatagt accataaatt ggatagtatc acaggaatgg
    14041 tcaagtggaa tagttggaat gcttggagca tcatatcttg gttatgtcca atgggcagct
    14101 gcatcttcag gcaataagca tcttaaagct ttagttagta tagtaacatc aggaagccct
    14161 tttattgata taccaagaaa aggaggagct tttgtatctg gtatgcttgc atgggcattt
    14221 atggtatcaa gaaataaagt tgatagaagc aaaatggtaa gagatgattg ggatgatgtt
    14281 ttaaacatac gaccaataga aaatatacct gttgaagctc ttggatatag aattgaattt
    14341 ctggaagaat ggttaaagag agtagaaaaa gatgagtatt gggatttgat ggattggcat
    14401 ttacaaaaag ataaaataaa tgtacctgca ttggttgtat ctgggtggta tgatgataat
    14461 agtatgggaa ctacagaggc cttagatgtt ataaaggact atgaaaaagg aaagagaaag
    14521 gctattctag gtccatggat gcataattca aatactttaa gagatataaa tggtatttct
    14581 ttaggaaata gttcattaag atatgatttg gattataatt atctattatg gtttgataaa
    14641 tacttaaaag gtatagaaaa taacatagat acgactgctc cagtagagta ttactctgta
    14701 ggttctaata agtggaagac agaagaaaat tggccaataa taaataagat agataaaagt
    14761 atgtatctaa taagtgatgg aaatgctaac acatctttag gaaatggtag attagtattt
    14821 gataatggtt tagaagaaaa gtatgatagt tatatatata atccaaaaga cccatcagta
    14881 caactaattg atatgtcaga gaatgaagtt ggagttccaa ataactacaa agatttagaa
    14941 aaaagaagtg atatgttgtg ttatacatca gatgctttta gtgaagaatt tactgtaaca
    15001 ggtgatataa agttagagtt ttttgcatca agttctgcca aagatactga ctgggttata
    15061 aaaattatgg acgtggattt agatggaaat tcaattaaat tagctgatgg tatacttagt
    15121 gctagattta gaaatagttt ttataagtca gagtttatgg aagaaggaga gatttataag
    15181 tttacgatta taacttcaaa aatatcaaac acgtttaaag ttggtcataa aattagacta
    15241 gatataacgt ctagtgctaa aaactttatt ttccaaaata gcaacacgac agagggatat
    15301 aatagtattg aatatataga agcaaagaat acgatttatc atggaggtaa atatccatct
    15361 aaattgatat tgccaataga gaataagtaa atttatatag attaaatagt gaggaggtgt
    15421 ctcaaaatga attttttagt tcgtgagaca ctttctttta tacaaaatca aatgtatttt
    15481 catcatttca acaatgaaaa agaggcttat ctctctgttt tgagatagcc tcctataagc
    15541 taaattgatt attttgtagc tgcaacaacg ctcatttcaa ctaacaaatg ctctctagca
    15601 agtctagctt caacacaagc tctagcaggt tcaaatcctt tttcaaccca agcatcccaa
    15661 acagagttca tagcttcaaa gtctttcata tctcttaaat aaatagtaac agatagcaaa
    15721 tgttgcttat ctgaaccata tttgtttaat aaatcttcta ttttagctaa aacttcagtt
    15781 gtttgttcaa ttactcctcc ttcagcatga gtttgtccac ataaataaac tgtattgtta
    15841 tgaactacag ctctactcat tcttccagtt ccttcatatc tctttatttc catattttaa
    15901 atacctccat agttaaaaaa tctattaaaa atctaaataa taaattaaaa aaagtctata
    15961 ctatataaaa ttatattata tattctgata ataatccata aaaacatata actttttagc
    16021 tataattatt attgttatta attgaaaaga tttattagtt acttgacata gtttacactt
    16081 ttaattatag tataaattgg aatatttaga taattattat ttaatgtata agctaagttt
    16141 ttaattaata tggttttata catttatata gaaaagaggt acgtatgagt agcacaatca
    16201 aatcaaatcg aataaaaggt ataatgttta taatagcatc agcgcttggc tttgcaatga
    16261 tgtctgcatt cgtaaagcta gctggagatt taccaagttt tcaaaaagtg ttctttagaa
    16321 atctggtatc agctataata gctttttggc ttatcataaa acataaagga agtctaactg
    16381 gtaaaaaaga aaatttaaaa gtacttttat ataggtcaat atttggaact ctaggagtta
    16441 tatttaacta ctatgcaatt gacaagttga tgctttcaga tgctaatatg ctaaataaaa
    16501 tgagtccctt ccttgtagta atattttgtg caattgctct aaaagaaaaa ataaatgtaa
    16561 agcagatagg agctattata attgcattta taggagcttt attcataata aaaccaacat
    16621 ttagtgtaga aataattcca tatcttgggg gaattgcagg agctgtattt gcagcaatgg
    16681 catatacttg tttaagagtt cttggagata gagaagatta ctatacaatt gtatttttct
    16741 tttcagtttt ttcattagtt actgtagggc caattgcttt tgcagtatat gaaccaatga
    16801 cccttatgca acttatatat ctattactag ggggagtatt tgcaagtcta ggtcaatttg
    16861 gaataactct agcatataaa tatgcacctg ctaaggaaat ctctatattt gactatagta
    16921 atataatctt ttcagcaatt ttaagtatat tcttattcaa tgtctatcca gatatgttaa
    16981 gtgttgttgg atatttaata gtattttcgg cagcatttta tatgttttta tacaataaga
    17041 agttagataa attagacaaa gaaaagaaga agtaaactag ataaaagtgt ttttataatt
    17101 tgtattgaat atttaagtct ttgtattaaa attaaagaaa atttaattaa aaatgtttgg
    17161 tatattaact tataacagca gttaaaattg aaaaattata actgctgttt cttttattaa
    17221 aaatatccaa atgtctgtaa aaatagacta taatagagta gtataaataa tataaagggg
    17281 ataaattaaa gtatggaaaa agtgaaagtt gctgtgattg gagcaggtaa tagaggaacg
    17341 tatgcatatg ctccatatat ttatgaaaat tctgatgtat gtgaaatagt tgctgtagca
    17401 gaacctaaaa aaggaagacg agaactattt acacaaaaat ataacctaga tagtaaaaat
    17461 gtatttgaaa ctttagaaga cttttttaaa catgacaaga tggcagatgc agtaataata
    17521 gctacaaatg atgatagaca ttatgatgta gcaaaactag ctcttgaaaa aggttatcat
    17581 gtactattag aaaagccaat gtctaatagt ttagatggtc tggtacatat agatgacttg
    17641 tgtgataagt ataaagataa gatttttatg atttgccatg tactgagata ttctccattt
    17701 tataataaat taaaagaaat tgtagaaagc aaaaaattag gagagctagt tagtatacaa
    17761 tataatgaaa atataggata ttggcatttt gcacatagct ttactagagg gaattggaga
    17821 aatagcaatg aaaccagccc tttaatttta gcaaagagtt gtcatgacat ggatattttg
    17881 ttatatttag taggaagtag atgcaaaaaa atatctgcat ttggaagctt aaagcatctt
    17941 aataaccaaa atgctagtgg agagatggca caaaattgtc ttcagtgttt agttgaaaaa
    18001 aaatgtccat attcagcaaa gagaatttat ttagaaaaag atagaagtat aaatagagct
    18061 gttcacataa atccaactga agagaatcta ttaaatatac ttaagacaag tccatatgga
    18121 agatgtgttt acagatgtga taataatgtt gtagacaata tggtaaatat actacagttt
    18181 gaaaatggag tgacggctac ttttaattta tgtgcattta ctaaagaaaa tggaagaact
    18241 ataaagttaa tgtttagcca tggtgaagtt ggtggggatt taaataaaaa tgagataaga
    18301 attaaagaat ttggaaaaaa cgaagaaata gttatgaatc catcaaatca acagaatatg
    18361 gtagaggaat atgatagaaa tcttatagct gaatttatta aattagtttc aaataaagaa
    18421 ctagaaaagg gtagagtagc agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca
    18481 gcagaatatt ctagggtcag tgatgaagta gtgtatatag aagaattttt tgatagtgct
    18541 aagcaaatga ctaaggagat agaagaaact atattttaat taataaaaat ttattaaata
    18601 aaataaagat agattaaata atagaaatac gaaaatatgt gttctattaa ggaatctatc
    18661 ttttttatta aggttttttc ttatccctat caaaaaaatc catacattct gataaaagat
    18721 aagaaagtaa agcgaataag atttctgtac ttaaaggaat cgttactgaa tccataaagg
    18781 tatcaattat gttcatagct agaaaagtta gtataaaatc aattataaat tcgcaaagtt
    18841 tatatattga gtatggtaat ttgtttacat atctgaatat atctaaaata gtagtgcata
    18901 gaaaatcaat tggagtggtt atacaaaaat atattaaaaa gaaaattaat acagctccaa
    18961 gagatttata ttcaaatcct aagaatgata agaagccaat tcctataacc gtgaaaatac
    19021 aaaaagttat accaaaaaaa acagcaaaaa ataaaaatac agaaataaat tcgcctaaag
    19081 gaatccttct tttattactt atcttaatca actccttaat ttatgatatg aaatttatat
    19141 aaataataac attcttaaaa attcataata gtcctctgtt tcaaatagta ctgtttgaga
    19201 atctattttt ttcatatttg gataaaaaga ggccttaaaa gctttataat gttctctaaa
    19261 ttttatttct atcttaaatt tatctggcaa ttttattaag tgcctagata aatctccaga
    19321 taaagctttt ttgacacctt tttcaatttt ttttagtgca aggtttggat ggattgaaat
    19381 tgaaccattt ccaattcctt ttgacacagg aacagtgact atatttggat ttagtttttt
    19441 tgcactctca caaagcattt catctccact taaaaatgct acaggaacat tataatatgc
    19501 agcaatataa gcatttatag taaattcaga tgctatttca tcatttattt taaaatagtc
    19561 ataatcaata tccatagtat gggaaagtgg attaccatta caagtagctc cagagtgata
    19621 gccggtgaat atacatgcat caaagctttc atcaattcca gccatcataa ctagtgggtc
    19681 tttggtccat cctctaagta ttttgacgtt ttcaggtaaa atatttggat taatatttct
    19741 accactgtca tgtgcatctt taacaagtat ttcatgtaca ccagattgag tagctccatc
    19801 acatgctgct gttatctctt tactcatttg tgttctatag tgttgcgaaa attcacattc
    19861 aagttttgtt tcatcccaat ttacaatacc acaagtacct tctatatcag cacttaaata
    19921 caatttcata atatataccc ccttatgttt attattgatg ataataacag ataacagaaa
    19981 atacataatc aataaagggc ataaaaatat tatgtcttac tattaattat tattaaatct
    20041 gtaactgaaa aataactaaa ttggtattaa aaaatataac actatgtgtc atttatttat
    20101 atagttttta tatctgatgt ttatataaaa ttgtaacata taagtggt
//
LOCUS       DAELTA010000068        19999 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.068, whole genome shotgun sequence.
ACCESSION   DAELTA010000068 DAELTA010000000
VERSION     DAELTA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.068"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(24..1256)
                     /locus_tag="KPG13_002007"
     CDS             complement(24..1256)
                     /locus_tag="KPG13_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985739.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(1246..1917)
                     /locus_tag="KPG13_002008"
     CDS             complement(1246..1917)
                     /locus_tag="KPG13_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985740.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(1933..2769)
                     /locus_tag="KPG13_002009"
     CDS             complement(1933..2769)
                     /locus_tag="KPG13_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985741.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(2795..3469)
                     /locus_tag="KPG13_002010"
     CDS             complement(2795..3469)
                     /locus_tag="KPG13_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0985742.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(3483..4211)
                     /locus_tag="KPG13_002011"
     CDS             complement(3483..4211)
                     /locus_tag="KPG13_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0985743.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(4338..5027)
                     /locus_tag="KPG13_002012"
     CDS             complement(4338..5027)
                     /locus_tag="KPG13_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0985744.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(5035..5598)
                     /locus_tag="KPG13_002013"
     CDS             complement(5035..5598)
                     /locus_tag="KPG13_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0985745.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(5600..6568)
                     /locus_tag="KPG13_002014"
     CDS             complement(5600..6568)
                     /locus_tag="KPG13_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG0985746.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(6669..6712)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            7078..7707
                     /locus_tag="KPG13_002015"
     CDS             7078..7707
                     /locus_tag="KPG13_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985747.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            7682..8086
                     /locus_tag="KPG13_002016"
     CDS             7682..8086
                     /locus_tag="KPG13_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG0985748.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            8089..8565
                     /locus_tag="KPG13_002017"
     CDS             8089..8565
                     /locus_tag="KPG13_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG0985749.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            9137..9802
                     /locus_tag="KPG13_002018"
     CDS             9137..9802
                     /locus_tag="KPG13_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG0985750.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            9824..10756
                     /locus_tag="KPG13_002019"
     CDS             9824..10756
                     /locus_tag="KPG13_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG0985751.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            10777..11418
                     /gene="pcp"
                     /locus_tag="KPG13_002020"
     CDS             10777..11418
                     /gene="pcp"
                     /locus_tag="KPG13_002020"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG0985752.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(11538..11870)
                     /locus_tag="KPG13_002021"
     CDS             complement(11538..11870)
                     /locus_tag="KPG13_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985753.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            12136..12756
                     /locus_tag="KPG13_002022"
     CDS             12136..12756
                     /locus_tag="KPG13_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0985754.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(13081..13905)
                     /locus_tag="KPG13_002023"
     CDS             complement(13081..13905)
                     /locus_tag="KPG13_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG0985755.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(13916..15319)
                     /locus_tag="KPG13_002024"
     CDS             complement(13916..15319)
                     /locus_tag="KPG13_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985756.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(15323..16048)
                     /locus_tag="KPG13_002025"
     CDS             complement(15323..16048)
                     /locus_tag="KPG13_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985757.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(16160..16933)
                     /locus_tag="KPG13_002026"
     CDS             complement(16160..16933)
                     /locus_tag="KPG13_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985758.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(16939..17826)
                     /locus_tag="KPG13_002027"
     CDS             complement(16939..17826)
                     /locus_tag="KPG13_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985759.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(17840..18862)
                     /locus_tag="KPG13_002028"
     CDS             complement(17840..18862)
                     /locus_tag="KPG13_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985760.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            19437..19613
                     /locus_tag="KPG13_002029"
     CDS             19437..19613
                     /locus_tag="KPG13_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985761.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         6980 a         3101 c         2166 g         7752 t
ORIGIN      
        1 aaaaacaatt ttttatattt ttattatttt aacttttttg gtattgacaa ttcaaatgta
       61 gttccaatat ctacatcaga agttacattt atatcaatag ataagtttaa actaatcatg
      121 cgagctattg aaagtcctat tccagaacct tctaaatctt tatttcttac attttcactt
      181 ctaaaaaatc tatcaaatat aagaggtatc tcttcttttt ttatgcctat accagtatca
      241 cttataagta aagttacctc atcctctata tcttctttca attctaatgc tatctcatca
      301 cccaattttg tatacttaaa ggcattatca atgaaaatta atataagttg tcttaattta
      361 tttttatctg ttattataac tttattcttc agtttagaat taaacacgaa tcttttttcc
      421 tgaaactcag caatgtcaat atagtcttct gatatttctt ccaataactt ttctaaatct
      481 atttcttcta aattaacttt aacaatagag tcctctttag taagagacaa taaatcagtt
      541 atcatttttt taagtcttct agtttccttc attgcagtag ctattgtttc aacttcatca
      601 tttactgtac tttctgggga ttttaacata ctttctaatt ttgatgaaac tattgttatt
      661 ggtgttctta attcatgaga agcatcttgt ataaacttag cttgattttt ccatgctgtt
      721 tctattggaa ttaaagcttt tctagtgaga tatacagcca caaagtaagt aattattaca
      781 gaaattaata ttccaattat aagaacagat gtaagctgtc ttaaagaatt catctcagaa
      841 tctatatttc taattatctg tatttggtat ctacctacat ctacatttaa ttctctaaaa
      901 gtatatccat tttctgtgta tttaaaaaac gaattttttt tatctagtct tctatctgga
      961 agtaactcat caaaatattc attttgagta tagtaactta tccttccatc ttcatataca
     1021 tacaccatat ctttaggatc tttgagtctt attgggttca aaaatgaagt tcttttaaat
     1081 tgagagacta tatattcaag ttcatcattt atctcactat caattccact atatgtaagt
     1141 cccctaaaat atgagtatat aaatatagaa aagattatta aaaaactacc tactacaata
     1201 atatacattt ttattaaatg atttttagta ctggtaaata cattcttatt tatcatcgaa
     1261 tatatatcct accttacgtt tagttttaat atatttatca tatccatatt tacttagctt
     1321 ttttcttaga ttacttacat atacttccac tatttcaata gttgcatctg aatcaattcc
     1381 ccatattcta tcataaatct gctcttttaa taaaattgaa cctttattta atacaaaata
     1441 ctcaagcaag ttaaactgtt tgttttgaag ttttatttct tctttttcaa tatacactct
     1501 tttttctagt gtatctaaat ataaagattt aaattctaat ccgtttcttt cttttatctt
     1561 tccattagtc cttaatatag cgtataccct agctactaac tcttccatat aaaaaggttt
     1621 tgtcaaataa tcattagctc ctatagtaaa tgcttctact ttgtcatcta atgtttcttt
     1681 tgctgtcaat ataagtacag gtgtatctat attattaatt ctcattttct ttaggatatc
     1741 aaatccattt atattaggaa gcattaagtc taaaatgact aaatcatata tattttgatt
     1801 tattaagtat aaagcttctt ctccatcttc acacttttca gtttcaaaat gcttgctaag
     1861 ttcttctact acactttcta aaagtatctt attatcttca actattaaaa ccttcataag
     1921 tatcccctac ctttatttgt ctatcttaac tttatgagat tttaagtcaa tatccacatt
     1981 ggctttttct tttatatcat catacgtata tcccactatt tcaacacttt ttgcctttgt
     2041 tgaacacaat atatctatat ctgtctcttc tgaaggcttt aaaatcttat atgatggtac
     2101 acgatttact gttaaatatt ctccattttc attaacttct gctgtcttaa gaataatatt
     2161 tcctaaatct tttgatgatg aattttttac ttttacttga tacgtaattc cctcatttga
     2221 ttcatttact tttttaagtt ctgatgtaga tagcacctca tactgactac tattttctag
     2281 cttaatatta ttatttctta tattaatagt attttctttt aaattaacat acacaatctt
     2341 accctctgcc tcatattcat atgcataaac ttctacaccc tttacaaaag ttttatgccc
     2401 acattctatg taagcaactt tcccaggcat taaagtcatg tccaacaaag ccttactatc
     2461 cgaagatata atttttttat tctcatcata ttcattatat atcaattcaa cattggatat
     2521 attaaatgtt gaattatttt ctacctttgt tgatgtatct actttatcac cttcatctat
     2581 atttataatt tctgattttc ctatattcaa tacttttgca tttccctcat ctatttttat
     2641 ctcattttta tcttctgttt gaatatcaac tgaatcaggc tgttcatctt tttcacatgc
     2701 tgtcaacata aaaataaata ttgttaatat aatcattaga gaaattgatt tttttactat
     2761 tttattcata ttaagcacct catatagtag tattttatag tagattttta tactttttca
     2821 taaattcttt caatgcctgt gtaagtattt gatgtttttc aaaattcgaa ttactacata
     2881 atctttcaaa atcattccat atttctttat ctatacgtat tcttgttgtt tttaattcat
     2941 ctatgtgttc atcatctata tgtacatctt ttttattaga tactttttta ggctttatat
     3001 tgtaatcttt tacttctaaa taccaatcat atacttcaca aagcttatct aaatcttctt
     3061 gagttatatt tatttttcta tctttcttag gcttaatatt ttgttttggc tttgcatttt
     3121 ttaaacctgt atcattaaac tcaatttgtt caacactttt acttaaagcc ttttcattag
     3181 aaatcttttt tttggtgctt actttttttc tattacttat agtattcttt ttactttttt
     3241 catactcctt agctttctct tcttccaagt ttctattatc caaactttta ttataattta
     3301 attcgtcttt taatatgtat ttaccttttt ctgacttata tcctttttta tttaaaaaag
     3361 ttcttaaagt actttgacta atctttagat tttgagctat ctctgcaaat ttaaatcctt
     3421 tgctttgaag ttctaaaaat ttgtcaattc tgtcttcatt gttattcatt aatattcctc
     3481 cgtcaacatc ttcttgtctt tcttttcata cttcttatta tattcttctt ttctaaatct
     3541 actctatttg attcaattat tttttgtagc attttattat aagtgaaatc atctaaatta
     3601 ttatttttta tatagagaaa agtttcctct tcacatttaa taaaacaaat tgatagcaac
     3661 catgccatac ccattttaac ataatattct tcactttgta tattattaca gtattcaaat
     3721 atttctttaa tatgtttatc atcaacataa tatattaaaa acataactaa tgcaaatcta
     3781 atttcccatg gattctttga ataaacatat tttttaagta tatcatacat attttcttta
     3841 tattttttca tagactttaa atcagaacaa aatccatcgc aaactgacca attatcgatt
     3901 ttaggtataa atctttttac atagtataaa gtttcttcaa aattactatt tatattgcca
     3961 attaccatac cttgtagcct aacttcttca tagtatgtat cctgtgctac ttctaaaaat
     4021 tccttccaat tcccttggct tatttctttt gataactttc ttaaattagg aactctaata
     4081 cctattatat tttctgttcc aggtataagc tttttattaa aatctctata ttttatatct
     4141 tgtatagaca tcatatattc taaaagttca ttataatcca atttattcca ttttccccta
     4201 tcaaacatca tttaattgct ccttaattcc ttttttctta ttgtaacata aaactctaca
     4261 aaaaaagctt ctaactatta gaagcttttt caaaaacata agaaactttt caattttaaa
     4321 tttaaactaa attaatgcta atctcctaca ccaaagtctg tattattgta taaaacttct
     4381 atatttggat tttcatccaa gaatttatac ttactaacaa accatttcac taactcttca
     4441 tctcttttta tactagaaga attatttata aaaacattca ctgtcgcttt ttcaaaagta
     4501 tttaggataa tctctatatc tttatctatc atctcttttg tttgtccttt tattccaacc
     4561 ataatacaag gtgaatcaaa atattctttt acatcttgtg gagtcttaaa atttgcattt
     4621 ttatttaaaa tattatttct aaaatcattg tcaaaggttt caactccaat tttaaatacc
     4681 acaggaattt caaaatactc tctcatttcc ttaagcctat ttttgtaaga ccaatgactt
     4741 tctaaaaata atttttttat attcttctct tttataatac actttatttt ttctaaagta
     4801 tccttaggta attcaaaaca gcttccagaa tttatgactt caagaactcc atatttccca
     4861 gttacgtttt ttaaaacctt tagatttaat ttattcatct cttcttctaa attagaatta
     4921 tcatctatat aatcacaaaa agaacacttt ccccatatac aaggaaatcc tttaagcaga
     4981 acaatttctc tttgattttt atttgttatc tcactatatc tatccatttt ctatctattt
     5041 tcctccagtc aatgatacct ttaaagtttt tggaactgca tttaatccta aattcactaa
     5101 aacaactgct aacagcttat ctccataatc agtaaatact tgggttacaa acacactaaa
     5161 aacatccccc agtcctaaca ctttcaagat ttgcactata tatgatgaac ctgaagaggt
     5221 catacctcca aataaaaatg ctgaaattat tgaacttatt atagatgttg gaatggcaaa
     5281 taaaagtact cctagtggag tttttaaacc ccttaaaaaa ttctttttaa atgctattcc
     5341 agcaagtaat ccggttgata tttgaactgg tgcaaaatag agagaataca catcaaacgt
     5401 aattccactt attaaacttc caaacacacc tgtaacaaca gcgtatttag gtcctaaaag
     5461 tgaagctatc attatagttc ctattgaatc taagtataca ggtaatctta atcccatagc
     5521 tataaaagcg cctattatat ttatagaaat accaaaagct ataagtgtaa tagtttttac
     5581 acatattttt cttttcacac tatatcactc cttccacaga gtctataatg tcttcataac
     5641 ctttaaaaag cctctttaaa aacatgtaca taaatttctt ctcatcaact tcatttagaa
     5701 caattgcatt aggatttttt ttatagaaat tgaaagaatc cactatagac tgacccatag
     5761 ctattccatc ttctacaact tctacatatg attcaaatcc tttacatata cttctatcta
     5821 taaagtatgc tactgctaga gggtcattta tcacacagcc aattattccc tcctgttccc
     5881 aatggaaatc aatataaaat ctagttattt cagttatata ctttgccatc ttcttatcaa
     5941 gtctatttat aaactcaata atattaggag taagtacaat ttttctagtt acatctaaac
     6001 ctaccatgtg gattttttta gataaattct tgtaaacata atctgctcca tgtgggtcta
     6061 cccaataatt aaactctgct actggagagc aatttccatg aatcctaaat gctcctccca
     6121 tagatacaaa ctcatcgaga ttttcaaatg ctttcttatc tttaataagt gctttagcta
     6181 tatttgtaag tggtgcaagt gctattattg atactttttc atgattatat aaagtattta
     6241 ttataaaatc cactgctcca ttttttgctt tagctcctac aaccttttga taaaagtttt
     6301 ctccaatacc atcttctcca tgtgtatctt gagctgttac aagttttctt tttagtggtg
     6361 cttcttctcc tatatatact ggaatattta gtgaagaaca catttgaagt gtttttagtg
     6421 cattttctgc acctatattt gctgggacat ttccacaaca tgtggtaatt ccaattactt
     6481 ctagctctgg tgagtttaaa gccagaagaa ttgccaaaga atcatcaatt cctgggtcac
     6541 aatcaattat tacttttctc ttttccattt tttatcctcc ttgatttgtg gtcgaggaga
     6601 actacacatc ttttttccac tttcaaaaag gtgtgtctaa gggtattaat tatatactgc
     6661 cactatataa ttccttagtt ttttatactt ggagttctcg aacaagtaca gatgtaaaat
     6721 catattttta ggctacttac tataagactc ttaaaaacta tagaccgtta aaaaattttt
     6781 aaacatctga tttaatagct ataaaataaa ttttatagca taaaagaatt tattatattt
     6841 taatgtatct tattaaagtt tatttagaat tatgaaccaa ctttaataag atgtggcgca
     6901 tttaaaccga ttattattat acaataatat cttaaatatt acaattaata acattaattt
     6961 attatccata aaaatatatt aacttatatt atttaataat ttattgacag tattaaaatt
     7021 aaattataca ataacagtgt aaattaactt aacaccgtta agcaaagagg tgattatatg
     7081 agtatcaaag aaagacgtaa aaatgaaaaa gaagaaatga agaaaaagat tatggatgct
     7141 agcatcgaaa ttataaacca acatggatat gaaaatttat caataagaaa aattgctaca
     7201 aaaatagaat attctccaac tacaatttat ctttactaca aagacaaagc agagattatc
     7261 agcgatatga ccaataaact ttataacaca gtagaaagta atgccattga cattatgaat
     7321 aattgctcat cactaccaat agataaacaa atcatagaaa ttatgactag ctttataaaa
     7381 actctttcta gtgaaccaga gatggcaaaa tctatcatgc atagtagaat gaatattata
     7441 tttgcatctg aaaatacaaa caatacgcct agcaataatg gtataaaaat gcttgataaa
     7501 ttcctttcca ttggaattga acaaggaata ttcaaaaaaa atatagataa aagctcatgg
     7561 atgcttataa gtgcattgtt aggattcgtt ttatgtgttg ttgaaaataa actatattct
     7621 ttacataatt tttctcaact tatagaaaat ttcttagata ttttagtggg aggactttac
     7681 aatgaaaatt cgcaacaagg ctactaataa aaagaaaaaa tatgtaaaaa tatctattgc
     7741 tctattcttt gtatttatat gcattattat tgtaattgta agcaacttaa agccagaaaa
     7801 cttggtagta aaagatatag atattaataa tgttaaaaat ggcatttata ctggaagtgc
     7861 tgataataac ttggtaaaag ctactgtgtc tgttgaagtt aataatggta aaatccaaaa
     7921 tattaatatc ttaaaacacg accatctttt aggtaaacct gctgaaaaaa ttacaacaag
     7981 tattattaaa caacagtctt tagatgtgga tgctataact tctgcaactt atagcagtaa
     8041 tactatacga aaagctgtag aaaatgcttt aagaaaggga gaataattat gaatactatt
     8101 attatatatt caagtaaata tggttgtaca aaagactgtg caaatatact aaaaaataaa
     8161 ctatcagata atgtcactat tgttgatatt aataataata ataataaaat tgagttatca
     8221 aaatttgata aaattattat tggaagttct atatatgttg gttcagtttc aaaaaaaata
     8281 caggtgctat gtaatgataa tgtagagtta ttaaacaaaa aacaagttgg cattttcctt
     8341 tgctgtggct tttctgaaca agcagataaa tatttaaaat caaattttcc atcatcctta
     8401 ttagaaagtg caaatgctat tggaattttt ggtagtgaag ctcgattaga aaaaatgaaa
     8461 tttttagata aacttattat gaaggctgtt tcaaaaggta attatgacag ttttagaata
     8521 tcacaggata atatagataa ttttttaata aatcttaata gctaaaataa agctaattat
     8581 ttctatgata aattctttta tttataagta atattgctca aaatagtctt taaactttat
     8641 caaaatttta tttttcttag tgaaatctaa tgaaactagc acttaatata tgctttatta
     8701 atcataacaa atatgtttta ttaatcataa caaaaagaga gtgtctaatg aactaaaaaa
     8761 ttcattagac actctctttt tgtatgaaat caaatatatt tttatcattt caacagagaa
     8821 aaatacactt gtctctatgt ttggcacatt ctctttttat aatataatgt ttaatttaaa
     8881 actactattt caaatattga catatactta aactatagac ttacaaaatc aattcatatt
     8941 agcttaaaat ataatctttg ttattcaaat tcctatacac aaccattatt tactttgata
     9001 ctttaaaaca tcttccctat tttttattaa attttataat aatagtccta ttaacgcaaa
     9061 tactataaaa agagtttaac tttatccatt aaataaattt aaattaaaac atatagaaat
     9121 ggaaggagaa caatttatga taaaactaat cggtatattg atagttgtaa ttggatttat
     9181 acttaaaatt gacacactat ttactgtatt attagcagga gtggcaacag gaattgttgc
     9241 aggattagat tttaatcaaa tttttacaat tttaggagat tcttttgtct caaacagagg
     9301 tgtatcatta ttcatattaa cattgccagt aatcggtgta ttagaaagat atggtttaag
     9361 acaaagagct gtttctttaa tagaaaaatt aaaaagactt acaacaggaa aagtacttac
     9421 tatatacatg atagctagac aaatagctgg tgctctatca ataagaatga gtggacatcc
     9481 tcaatttgta agaccacttg ttaatcctat ggcacaagct gctggtttaa gtaatagtga
     9541 tgaactaaaa gaatcggatg aagaagccat aaaggcatta tctgctgcat cagaaaacta
     9601 tggaaatttt tatggtcaaa acttatttgc aggtagtagt ggagtattac ttatagcatc
     9661 tactctgact caatttggat acaatgtaac tggacttaat atcgtaaagg ctagtactat
     9721 tatggcagta atagcacttg tagtggctac aatccagttt accttatatg ataaaaaatt
     9781 aaacaaaagt cataaaaaat agttttattt aggaggaata aaaatgcaaa attttataaa
     9841 catatcattg gaaatctttt acgcactaat gggattttta atgattgtta tagcatataa
     9901 gtcttttact accataaata acaataagaa atatggtaca tcattatttt ggatacttat
     9961 atcactgcca ttcatatttg gtagattgat tccagccaat ataataggaa taatattaat
    10021 attatctagt ttattaacac tttctaaaca agttgtattt gcaaagtatg aagaaccaga
    10081 tgaaaatttt ggaaaagaac aagcagacaa acttaaaaat aaaatattta taccatcatt
    10141 aatattagca tttgctgcag tagtagtagc tatgagtctt tctaattttg ataattcttc
    10201 tcagtttgca ataggtgtgg gttcaataat tgcattaata tcagccctta taataactaa
    10261 ggcaaaacct gctacatcag ttcaagatgg ctcaagatta cttcaacaaa tgggacctgc
    10321 tagtatgtta ccacaacttt tagttgcatt gggagcttta tttactcaag ccggagtcgg
    10381 tgaagttatt tcaaccatga tatctggtgt agtccctgct gatagcagat tatttggtgt
    10441 tatagcttat gtattaggta tggttatatt taccatgata atgggaaatg catttgccgc
    10501 attttcagtc ataacggcag gaattggtat tccttttgta ttgtctcaag gtggaaatcc
    10561 tgctataatt ggagcattag cattaacagc aggatattgt ggaactctac ttactccaat
    10621 ggctgctaat tttaatatag ttccagcagc cttgctagaa tgtaaaaatg attatattgt
    10681 tataaaatat caagctccag ttgctttggt attaattata gcccacatat tagtaatgta
    10741 ctttttagga ttttaatata attaggaggt aaaataatga aaattttgtt aacaggattt
    10801 gacccatttg gcggagaacc aataaaccca gctcaagaag cagttgaaag ggtcaataat
    10861 aatataaacg gtgcagaaat cataaagata actataccaa cagttatgac aaaatctgtt
    10921 gaagctatag ataaagctat tcaagagcac aatccagata tagtaatatc agttggacaa
    10981 gctggaggta gatttgacat tacacctgag agagttgcta taaatataga tgattttaga
    11041 ataaaagata atgagggtaa ccaggttata gatactatta taaaagaaga tggtgaacct
    11101 gcatattttt caaaattgcc tgtaaaagct atggtaaaac atatgaatga aaataaaatt
    11161 ccagctagtg tttcaaacac tgcaggaaca tttgtttgca atcatgtgat gtatggaatt
    11221 ctttatatga tagacaagaa atatcctaat ataaggggtg gatttataca catcccatat
    11281 acaacttcac aagtaataga taaaaaaaat acaccattca tgtctttaga agaaatagta
    11341 aaaggcttag aattagctat agaagcttgt attatatata aagaagatgt aaaagaaatt
    11401 ggtggtgaaa tttcataata aattttttag tatttttata aattaaaaat actaactatt
    11461 aatttaaata tagactgtga gtagattatt caaacaattt tactcacagt ctttttatgg
    11521 tatctacaga tacaaaatta aggtctattc ctctctcccc actcacacat tgcatctaaa
    11581 attggaatca gagattttcc cctttctgat agactatatt ctaccttagg aggtatttga
    11641 ggatattcag tacgaataat caagttatct gcttctaatt cttttaatga taaactcaaa
    11701 gttttatagg agatagtatt tatataacgt tttaatgcat tatatcttac aacttcaaat
    11761 tctacaagac agtaaagaat tatcatctta tatttaccac taatcagtga aagtgtataa
    11821 ctaaatcctg tttcacttaa atctgtattt gatatacagt ctatactcat atattacact
    11881 ctccttttgg ttagtattga acaaaaatgt gcgtacttgt ttttagaata atttataata
    11941 aaattatagt gtaagaataa ttgaaagtca attttataat aggaggtgta tttactaaaa
    12001 aaaaagttat tttaaatagg attctaagat tagacatttc tatataaatt gataatattc
    12061 atgaaaatcc tattttataa aaaataatgt ctcttagaaa atctattaaa taaaattcat
    12121 tggaggaaat ttataatgaa agttttattg ataaacggta gcccaaacca atatggatgt
    12181 acttatacag cactaaatga gattactaaa gtattgagta aacacgatat taaaacagaa
    12241 atactgtatt taggtaaaga aacaatccca ggatgtatta gttgcgcatc ctgttttgaa
    12301 actggaaaat gtattcgaaa tgataaagtt aatgaattga ttgaagatct ggataatatt
    12361 gatggtatta ttattggttc tcctgtttac ttttctagtg caactggtca attaacttct
    12421 tttcttgacc gtctattttt catagctggg agtcgcatgg ctacaaaatt aggtgcttca
    12481 gttgtgtcat gtagacgtgg tggtgcaagt gcaacatttg accaactaaa taaatatttt
    12541 tcaatcagca atatgcctat tgtatcatca cagtattgga accaggtaca tggctttact
    12601 ccagaggatg ttatgaagga tgaagaagga ttacagacta tgcgaactct aggagaaaac
    12661 atggcatggc ttcttaagtg tatatcagca ggtaaaaaag ctggaattaa agaaccacaa
    12721 tatgaagaaa caattatgac taattttata aaataaataa aaaatgtact aatattaatt
    12781 aaaacaaaac actaatttat aaataaaata agcataaatg taggataaaa agcactaaaa
    12841 cagaggtatt gaattttata tcaaacctct gttttattat tacattaata accaaaccat
    12901 gtataacatc atatatttct aaataaatta taatattata ttctaatgtt attgaaatta
    12961 caataactga atttctgctc ataaatttaa atattctaca aatctcatct aaataacaat
    13021 tttaatagac ctaaaacaat cctcatactg ttcattttgt attaaaaata tcacttacaa
    13081 tcaaaaacca tttattaagt atattcctaa gatatacaat atatatacat ggattggata
    13141 gtataaataa aataaatact tcatactctt ccctttttct ccattgtaaa gtaacattaa
    13201 aggtagagaa gcaatcatca tccattggta attttgtaaa atagaatttt ttaaactata
    13261 atctccattt gacattgttg taaatataaa tatacacatt aaaacataac aaatactcaa
    13321 actgaacttt ttacctctac acaaataaaa aataatccca agtataatcc aaattggtcc
    13381 accttctact aaaaatggta ctggtaccaa aatcataaat aaagcaataa ctctgttagt
    13441 gactttaaaa agtaaagcta ctgtaaatat tcctaaaatt ataggaatga ttatcatcaa
    13501 taatcctata ataaaatatt taatttcttt ctcttttcta aatttcttta tgaactctat
    13561 tgcttgtaaa taaatcgcta ttaaaaataa tgttgaaaaa gtgttattta taattatagc
    13621 tccttctgga tgtgaaaata cattattaat tatttgattt cctatagcca taataacaga
    13681 ccaaatatac aatctactta tatatttttt tctgttacta gtgtgataaa atccttcaac
    13741 agccatgaat ataaacagtg gtgctgatat tctgcctacc caatgaaacc aaattggtat
    13801 ttgcattgtt tgtggcataa atgctgctat atggtcaaat gtcataaata ttaatgctaa
    13861 aatcttaata gtaaacccac ttataccttt tttaaaaaaa tccatatcat atctcctaat
    13921 tttgttttat ctctataata atttttgttc ctattccaat ttcactactt acataaattt
    13981 tacctttatg aatctctatt atttctttag aaatagccat accaagacct gaacctttat
    14041 gcatctcacc tgtattagta cctctataat attttttaaa aatatgtttc aaatcacttt
    14101 tgcttattcc aattccatca tcttttatta taatttctat attatctttt tttacaattt
    14161 ctacacttat caaagttcct tcgctattat gaactattga attaaatata agattggtta
    14221 tagcccttct gaataagatt gaatctacat atacttcaat taaatcttca ttactttcaa
    14281 attcaatatt cctattttta tatctattat catttaaaat atcaattatt atatctctga
    14341 ctaaactgac taaatttatt tttttcttgt ccagaactat agtattattt tttaatcgag
    14401 tagataaatt taaatcatca actaaatctt ttatatactt agacttatta tatataattt
    14461 ctgtatactc ttgtatttca tcttcttcaa actcataatc tttgtcatta attatctctg
    14521 catacccttg tatagatgct agaggtgtct ttatatcatg tgttatattt gccaaccatt
    14581 cctctctcaa ctcttcattt tcttttctct caacttcatt tactcttaaa gtatcagcta
    14641 acatatttat atttttaaaa acttcttcat atatacctct gtccttaaga tataaactat
    14701 aatttccatt agccatagtc tcaatactcc atagtacgct tgaaattggt tttgtaagtt
    14761 ttctactaaa taaatatcct acacctaaag ctaccacgct atcaattact agtgttataa
    14821 tgataacttt atttaaagtt tttttaatgt tattttgaga ataggttaaa attattcttg
    14881 atatatctct actaggtata cctactatta tgtttagatg ttttttgttt aaatattttt
    14941 ctaatacaaa attaacatac tttgtttcta tatacttgta attattcacc atttgaaagg
    15001 gtgtatattt tttgggagta tcctttggca catttactcc ataaacttcc tctcctaaat
    15061 catttaaaac ctgtatccag gaatttgatt tttctaattt ttcagctcca atcttagata
    15121 gtttaaattc tccatctttt tcatataaat ctttttcaaa agaacgtgca aattcttcag
    15181 gattatttcc agaatctaca actttaaaaa aactattagt tgaaatcaca tacaatatag
    15241 atatgatatt tataattact actgatatag ctacaaacac aattgtaaat ataaaatttc
    15301 tagtaatctt ccacttcata ttctaatctt cctttactgc taatttatat ccaagacctt
    15361 ttattgtcag aatatattca ggctttgaag gattatcctc taatttttcc ctaatttttc
    15421 ttatatgaac cattatagta ttgtcaaatc caacataatc ttctccccac actttatccc
    15481 atagtttttc tttacttata atttgatttt gattatgtgc aaataaagat aggattttta
    15541 gttcctttgc agacagtcct atagattttc catcttttat aagttctgcc ctactttcat
    15601 ttaatatata tgggccaaat gatataattt tttcttcatt attatttaga tttttacttt
    15661 cgttttgagc atcactaaaa taacttaatc ttctcaaatg cgctttaact ctaaaagcca
    15721 cttccttagg gctaaaaggt tttgtaacat aatcatctcc tccaatcgct agtccaagta
    15781 acttatctaa ttcttcggtt ttagctgata aaaatattat tggagaattg gaggtttttc
    15841 ttatttcttt acaaatatca taaccttcgc catctggtaa cataatatcc aatacaatca
    15901 aatcaggatt aatactttta aataattcta atccttcttt tttagttttt gcagtatata
    15961 tattattaag gccctctttt tttaggacag tttctaaaag cttcaatatt tctacttcat
    16021 catctattat taaaacacga ttttccataa acaacctcct gtaataattt acatatatat
    16081 tagcatatat tttatctaaa attcatttat cttattttta attaaactaa aatataaata
    16141 aaattactat tatttttagt taaaaccttt tgcttgatgt aaaattttca tatgttattt
    16201 tagttaacac tatcattaat ataattgaga ttactataga aaaaactatc aaaatacttg
    16261 gtacattata cttaaccatt tgttggctca tcataatcca atttggagtc actgacataa
    16321 attttatagc tttatctggt ataaaaccta cactaaagta tggaattgcc attatcaatc
    16381 cacctagtat aaatggaatt acagccgatt taaatataga tgaaagtgtt aagcctattt
    16441 gtgcaaatat aaaagaagca aaaatagtag tcaatataac aattatatac atttgtatta
    16501 ctgtaagatt taaattacta tttccaaagt accataagtt atgaatcgat tcatttaaat
    16561 ttttaaaatc aaaaaaacta aaacctctaa ttcctactaa caatataaaa gaaatattaa
    16621 taatagataa aattaatcca ttagctacta gttttgataa tactactgat ttttttccct
    16681 ttttagttgt atttattaaa cctgtcatat tagaatcata ttctttaatg taatttccag
    16741 aagttattat aaacattatc aaacctatta gaggtattga gccttctatc ttgtaaaata
    16801 tatctgcata agtatctaaa acatcaaact ttctaatcag aaaaaatcca aaaaatatta
    16861 aaaacagcca aattatccaa acactctttg attttaatat ttttatcatt tcagctttaa
    16921 ctaaatacat catttctact acacctccct tgcatcttct aaatcaaaat aaaacatgta
    16981 tacatcttcg aattttgctt gaactggttg tatattatcc tcgcttggaa tatcctctga
    17041 aacaattcta agtactactg aatcaatatc actttgaata cttacaacct tatatttaga
    17101 ttgtatatca tcaacttcac attcattttt aacttgaaca ttatatactt tattttccat
    17161 gtgacttaat atgtctttgt gtgttccttt cattaggagt ttgccatctt taatcattat
    17221 tgtttccttt gcaactgatt ctatatcgga aattatatga gtagaaagta ttattatctt
    17281 atctctagac atttttgcta ttaaattcct aaatctagct ctttcttgtg ggtcaagtcc
    17341 tgctgttggc tcatcaagga ctattatctt agggtcgttt agtaaaactt gtgctatccc
    17401 aactctcctc ttcatacctc ctgaaaattt tcctatagac ttatttctca catcataaag
    17461 acctactaaa tctaataatt ctcctatctt actatttaaa tattttcccc ttaaaccttt
    17521 taaagctcca atatactgta aaaattcttt tgctgtaaaa tttttataaa ctccaaattc
    17581 ttgtggcaaa tatcctaaaa tatttctata atcagcatcc attttaaata tatcctcacc
    17641 attataaagt atctgacctt catcaggttt tatcaacgtt gttatttgtt tcattaatgt
    17701 agattttcct gctccatttg gtcctagaag accataaact ccattttcca gagttactgt
    17761 aatcttgtcg tttgctactt ttttaccata tatctttgaa agattttcta tttctaattt
    17821 attcatctta actccccttt tatctactta tataatctga ttttattact aaaaaacata
    17881 atgacatagc acctatcaat attgccatca aaagtcctat tgctatattg acaatgtcta
    17941 tattcacttt aaatatcata atatcaccta gataatttcc tattatttta ttaaaaccaa
    18001 atatcaaatc ataataattt acatttgata caactgaaag taatttttta ggtaatatat
    18061 ggattaccga cattatctta gttatagata taataaatat aaaaaaactt attgaagata
    18121 ttgaagactt acttataaaa gacccaaata tggctatcat ggcccatcct tcaaacatca
    18181 aaagctctat gcctatattt aatattacat actttgttat acttaaatta gttttcaatg
    18241 caactacatt gtctactatt ctaaaagatt ctaagtctcc attaacaggt actccttgtt
    18301 gcatacaagt tataataaat attcccatta aataaacaga ataaattact aatggaacta
    18361 ataaagcaac aaatatttta gactttaatg cttttttctt ctcttttgat gatagtatga
    18421 tatcttttac cgaagtaacg acttcatcta cataaatatt ggatataatt atagatataa
    18481 tgattaacat tcctacattt ataagtgaat ttgtgactct atagttaaat actttccaaa
    18541 aactaatatc cttatattca ttcacactta gattatcaag ttttttcttt gacattaatt
    18601 caattttttt agaaaattca cctgtatctg tttgatttga aaactgcttt aaaacattaa
    18661 tcttattttg aaatttctca ttacctacgt cgataccact tctcgtatct tctatacgtc
    18721 ctttttcttt atctatataa taactttctg tttctaaaac aggcgtaata aagatactaa
    18781 tcattagtat taacactaat atccctagag atataaacat actcttagat ttagccaatt
    18841 tttttatttc ccaatttacc aatatatttc tcctcctaaa tcattttaat tacacatata
    18901 tattaaaact taaatcttat ataaatataa tactaacctt aaataaagct taaattattt
    18961 tataatttat aggtgtgttt attattggag ttacatttat tatttttctg ggaaattata
    19021 cattttcaat tatttttatt atttttgata tttttattga ttggaaattt tattgttgtt
    19081 caaattgtag ttattcttta aatttaaaga tggagttttt taagaataga acctactgtc
    19141 cttattatgg aaagattatt aaatagttgc atataacttt atttatcttg aatcttattt
    19201 ttaaaaacaa ttataatttt agaatatttt ctattctcaa acaagcatgt tgaataatgg
    19261 agcatcttct agtattatat acttgtagaa taaaactaaa ttggtaaaac tgggaatatt
    19321 taaccttaag gagttaagtt atagaaacta gtactaaaaa attttataaa ttatgttaat
    19381 tgactaatag aatgcgactt tttattttta ctaaattttt tagggggatg gataatatgt
    19441 ttattggaga aatagacaaa tgtacacata tactaactgc ttacatcagt agttcatatg
    19501 attattgtaa ttttttagat acacagttag atgattttat atcagaatat ggagaaacgg
    19561 tagttgaaac ctgcttacat caagtcttat tattagtaag tagatacaat taaaactaaa
    19621 tttttctaga aaaacctttt tagatttatc tactctaata aatatataat tttttgtact
    19681 ttataagtaa ctataacttc tgctaaagta aagatgtatt gggagtacat ctttactttc
    19741 ccattataga agttaaaagc atacaccata aaattaatca tccaatacaa attatatatt
    19801 tcctatgtct aacttaattt acaagaatca ttctatatgc atattctgca atccactaaa
    19861 catggctcta aacctttact attttataca ttctctataa atcatacaaa tcatatataa
    19921 aatcccccta atatgtttaa accacctata aaataacacc aatatttaga atattcaata
    19981 tcaactgcat aactggtca
//
LOCUS       DAELTA010000069        19902 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.069, whole genome shotgun sequence.
ACCESSION   DAELTA010000069 DAELTA010000000
VERSION     DAELTA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.069"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPG13_002030"
     tRNA            27..101
                     /locus_tag="KPG13_002030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPG13_002031"
     tRNA            116..200
                     /locus_tag="KPG13_002031"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPG13_002032"
     tRNA            210..293
                     /locus_tag="KPG13_002032"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KPG13_002033"
     tRNA            317..391
                     /locus_tag="KPG13_002033"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KPG13_002034"
     tRNA            416..492
                     /locus_tag="KPG13_002034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KPG13_002035"
     tRNA            502..577
                     /locus_tag="KPG13_002035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KPG13_002036"
     tRNA            586..661
                     /locus_tag="KPG13_002036"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KPG13_002037"
     tRNA            664..752
                     /locus_tag="KPG13_002037"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KPG13_002038"
     tRNA            756..831
                     /locus_tag="KPG13_002038"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KPG13_002039"
     tRNA            838..911
                     /locus_tag="KPG13_002039"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KPG13_002040"
     tRNA            926..1002
                     /locus_tag="KPG13_002040"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KPG13_002041"
     tRNA            1020..1096
                     /locus_tag="KPG13_002041"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KPG13_002042"
     tRNA            1105..1180
                     /locus_tag="KPG13_002042"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KPG13_002043"
     tRNA            1188..1261
                     /locus_tag="KPG13_002043"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KPG13_002044"
     tRNA            1269..1345
                     /locus_tag="KPG13_002044"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KPG13_002045"
     tRNA            1358..1433
                     /locus_tag="KPG13_002045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KPG13_002046"
     CDS             1509..1922
                     /locus_tag="KPG13_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985762.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="KPG13_002047"
     CDS             2231..5662
                     /locus_tag="KPG13_002047"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG0985763.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="KPG13_002048"
     CDS             5904..7832
                     /locus_tag="KPG13_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0985764.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="KPG13_002049"
     CDS             8285..8749
                     /locus_tag="KPG13_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG0985765.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="KPG13_002050"
     CDS             8756..9250
                     /locus_tag="KPG13_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG0985766.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="KPG13_002051"
     CDS             9266..10291
                     /locus_tag="KPG13_002051"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG0985767.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="KPG13_002052"
     CDS             10278..12725
                     /locus_tag="KPG13_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG0985768.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="KPG13_002053"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="KPG13_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG0985769.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="KPG13_002054"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="KPG13_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG0985770.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="KPG13_002055"
     CDS             15440..16534
                     /locus_tag="KPG13_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG0985771.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="KPG13_002056"
     CDS             16663..18840
                     /locus_tag="KPG13_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985772.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19020..19871)
                     /locus_tag="KPG13_002057"
     CDS             complement(19020..19871)
                     /locus_tag="KPG13_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0985773.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
BASE COUNT         8067 a         2229 c         3727 g         5879 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccacc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaagagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aaaatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgagttag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agtataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcgttgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gagctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaataaag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gagagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaaattat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgagg ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagagccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagactaaag aagcttacaa aaataatgat
    17341 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tcgccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa atggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18421 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggcatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc ttacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18961 attctaatca aattttaaga taaactcttt tttattataa agataaataa catataactt
    19021 catattttag gtagattttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtat taattaagtc tttataaata tgagaaatac gcacatcatt taaatactct
    19201 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ttgtaagtgt
    19261 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19321 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19381 gatattttaa agttagtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19441 agattagctg caggaggaga actaagttta atttctctca tgttttctaa tataagttta
    19501 actttacttg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19561 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19621 gaggtatttt gataaataca tctagttgag tgaataattc cagaattaat gaacatacac
    19681 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19741 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca
    19801 aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta
    19861 tttctgttca taataatatc ctccttataa ttttaaatta ta
//
LOCUS       DAELTA010000070        19523 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.070, whole genome shotgun sequence.
ACCESSION   DAELTA010000070 DAELTA010000000
VERSION     DAELTA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.070"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            153..674
                     /locus_tag="KPG13_002058"
     CDS             153..674
                     /locus_tag="KPG13_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0985774.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            708..1484
                     /locus_tag="KPG13_002059"
     CDS             708..1484
                     /locus_tag="KPG13_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0985775.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1826..2779
                     /locus_tag="KPG13_002060"
     CDS             1826..2779
                     /locus_tag="KPG13_002060"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG0985776.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2797..3816
                     /locus_tag="KPG13_002061"
     CDS             2797..3816
                     /locus_tag="KPG13_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG0985777.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3847..4098
                     /locus_tag="KPG13_002062"
     CDS             3847..4098
                     /locus_tag="KPG13_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985778.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4380..6185
                     /gene="lepA"
                     /locus_tag="KPG13_002063"
     CDS             4380..6185
                     /gene="lepA"
                     /locus_tag="KPG13_002063"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG0985779.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6334..7671
                     /locus_tag="KPG13_002064"
     CDS             6334..7671
                     /locus_tag="KPG13_002064"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG0985780.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7904..9217
                     /locus_tag="KPG13_002065"
     CDS             7904..9217
                     /locus_tag="KPG13_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0985781.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9389..10564
                     /locus_tag="KPG13_002066"
     CDS             9389..10564
                     /locus_tag="KPG13_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG0985782.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10667..11686
                     /gene="hrcA"
                     /locus_tag="KPG13_002067"
     CDS             10667..11686
                     /gene="hrcA"
                     /locus_tag="KPG13_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG0985783.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11716..12336
                     /gene="grpE"
                     /locus_tag="KPG13_002068"
     CDS             11716..12336
                     /gene="grpE"
                     /locus_tag="KPG13_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG0985784.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12428..14275
                     /gene="dnaK"
                     /locus_tag="KPG13_002069"
     CDS             12428..14275
                     /gene="dnaK"
                     /locus_tag="KPG13_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG0985785.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14486..15640
                     /gene="dnaJ"
                     /locus_tag="KPG13_002070"
     CDS             14486..15640
                     /gene="dnaJ"
                     /locus_tag="KPG13_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG0985786.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16606..17541
                     /locus_tag="KPG13_002071"
     CDS             16606..17541
                     /locus_tag="KPG13_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG0985787.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17577..18950
                     /locus_tag="KPG13_002072"
     CDS             17577..18950
                     /locus_tag="KPG13_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0985788.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19138..>19523)
                     /locus_tag="KPG13_002073"
     CDS             complement(19138..>19523)
                     /locus_tag="KPG13_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0985789.1"
                     /translation="DFDFRLQLCSDLLPHIIKDNAENKFLNLFDFIRYKTLDLINEDN
                     ILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILG
                     FDLKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         7739 a         2148 c         3539 g         6097 t
ORIGIN      
        1 gttagaaggt tatatgtaat taatgtcagt gtttatatag aatagcattt gtagcattta
       61 ttatagttgt aattactatt tatatcatag acttgaatag tctaaaaaaa tagactataa
      121 tatttttata gactaaagaa agggctgata agatggacat agcaaaacta attttaaatc
      181 ctgcaaggct tcgtatttta caatatattc gactttatgg cagtgttcgt acatctgata
      241 ttgtaaagta tttaaatgat attcctcgtg caacagttta tcaccatgta aaaattttag
      301 aagagaacaa tatgattgaa gtaattaaag aaaatcgtgt gcgtggtact attgaaaagg
      361 tttatgcact aaaggaatat acaacatcta tggaaggaga gaccttcgtt gcactttcta
      421 cagcatttta tgtaggtctg atgcaggaaa tgaatgaata ctttagcaga aaagagcaag
      481 accataaaaa ggataatgta tttttctctt cagcattact atatatgagt gataatgaat
      541 atgaaaatct tcttaaaagt atagctgatt tattgaaacc atatatagaa caaaaaccta
      601 aatcaaatct aaaacttaga aaactttcaa ttatatcttc tccacctgta gctgataatg
      661 ataaaacaaa ataacatgca acccattttt ataaaaggag gttatatatg aaatttcatg
      721 agtttggaaa taaaaacaga ccacatatta tgttgattca tgggggaggg aatgcatggt
      781 ggaactattt gcgacaagca gaagttttgt ctaacaaata tcatgtgata cttcctacat
      841 tggatggtca tggagaagaa tatgaaacaa catacatttc tacagaggat acagcagata
      901 agttgatatc atatatagat gaaaattgca gtggacatct tttcgctttg tgtggggtat
      961 cacttggtgg tcaaattgtc atggaacttt tatctcgaaa acctaatatt actaaaaaag
     1021 caattattga tggtagtatt tgctacccaa gacctcttat ggcaaggttt tgtattgctg
     1081 gtattcgatt ttttggagga cttttgttta gtgagaaggc ttgtcgcttt caaattgcta
     1141 tgatgccaaa attgttacct gaaaatatgc aatatccaga taaaataaaa gcgtattata
     1201 tgacagatat gccacatgta agaaaagaaa cattctataa tatgtatcgt acttacatga
     1261 tgaactatac acttaaagag aatgttaaaa aaaccacagc acaagtaatg tactggtatg
     1321 gagaaaaaga aatgaagtgt gtcaaaaaat ctgcaaaact gtttcagtct tatgtgccct
     1381 cttgtgagat ttatgaagct aagagctata atcatgggta tttatcactt tatcttccaa
     1441 atgaatggtt ggaaattgca gaaccatttt tccaaaagga ataaaggtgt taggtgatta
     1501 ttgtataatt attgctgttt ttttatatag taaataaatt aggtataaat ataaaaaatt
     1561 attttagtta atatattagt gaaagcttaa taggaaaatt ataaatagat taaaggtagc
     1621 acctatataa atagttgcta cctttttctg tttgtaaata aatgatagat aagaaggttt
     1681 actgacaaat cactgactaa acccaatttt cttacattga aagccttaat tttacttgtt
     1741 ttaaataaat ttacaatttc ttgttataaa atactaaatt ttgttaacaa ttataattag
     1801 catttaattt atactggagg tttatatgat tagtgtaaga acagatttag ctttagaagc
     1861 tagtgaaatg tgtgaaaagt ctcaagaagg aagtagtatt cctggtgtaa aaatagaaac
     1921 taaagaatta gaaaattgca ttgtaactaa agttgaagta atagacaggc aaggcgcaga
     1981 gataatgaat aaagatatag gaaagtatat aactctggaa agtaatctta tgaagtttga
     2041 tgatgatgag tcaagagaag aaatgataag ttatttaaaa gaggagttag tagatatatt
     2101 tggccaagac aaaaataaaa aaaccctggt aattggtctt ggaaatagaa atattacttc
     2161 tgatgcgtta ggaccaaaga gtgtatcaaa aacacttgta actagacatc tttttaaaaa
     2221 ttataataaa gactatgatg atgatttcac tgaggtatca gctttaagcc caggagttat
     2281 gggtgttact ggaatagaaa ctagtgaaat agtaaaatca ttggttgaga aggttaagcc
     2341 agatagagta gtagcaatag atgcattggc ttcaagaaaa atggaaagag taaactctac
     2401 tatacagata tcaacagcag gaatatcacc tggtggaggt gttggcaata ctagaaaatc
     2461 tctaactaaa gaaactttag gcgtggatgt tattgcaata ggcgttccaa cggtggttga
     2521 tgcagcaact cttacaatag atgtcttaga tatggcaata gacaatttaa tagctcaatc
     2581 agaagaaaca gaaagttttt atgaaatgtt gaaaaaactt aaagaagaag aaaaatatca
     2641 tctaataaaa gattcgttag acccatatga caagaactta atagtaacac caaaggatat
     2701 agatgataca atcgaaaatc tatctataat aataagtgaa ggcttaaata ggtctttaca
     2761 tccaggtaga ttggtataaa ataaggagga gcaattatgt ttaaaaaatg cattaaagta
     2821 gtaaccttga cttttatatt agtatgcatt ttaccaggaa aatcattagc tttgaatcaa
     2881 gatgattttt taaagttttt agtaaattca tcttatccag aagctaaagt cgaaggaaat
     2941 gatacagaaa ataaaaagaa taataaaaat aaagaaacaa gtaaggaaaa taaggaagaa
     3001 agtaaagaag gaaacactaa atctaaagat gcaagtaaag ttgataataa aaaagagagt
     3061 gaaaaagaat atataaaact ttatgttggt aaagaaaatg ttccagatat tgagtcaaaa
     3121 aattcagaca caactgaaac taacacaaca tctagttcag attataagga tgatttaaga
     3181 gtaaccaaag aaaatccaag aattttgata tatcatactc atggatgtga gacttattca
     3241 aattcacctg atggaaatta tcattcaaga gataagaaaa attcagtaat ggaagtagga
     3301 agtgcattaa ctagtgcttt agatagcaaa ggatggggtg tagtccatac tactaaatac
     3361 catgactatc catcttataa taactcttat gcaagtagct taaaaacaat acaaagtata
     3421 ttacctaaat ataactcagt ggatatagcg atagacttac atagagatgc tagggattta
     3481 actaatccag ccactaagga aaaagaccat ttaaaatata caactatgat aaatggagag
     3541 agagtatcaa agttcttctt tgttgtagga ggtaaaaata caaatagaaa acaacttaga
     3601 gctttagcag aagatataac tgcttttgct gagaaaaagt atcctggtct agtatctcca
     3661 atagtagaaa aagactatgc tagattcaat cagtttgcag ttaagaatca tatgttagta
     3721 gaaataggga ataacgcaac aagtgtagaa gaatctaagg ctactacaaa gtatttagcc
     3781 gagattttgg atgaatattt taaacaaaaa aattagagct ttaagttata attaggagca
     3841 tgctaaatga gccgattaga aaaaaatata gaaagtaaaa gtaaactaag aaaatacaag
     3901 tatattatga gaatcttatt tttgattact atgattatat gtacatcagt ttgtgtattt
     3961 atagtggaca gtaatgcaaa aagtatgcta ggaatgaaac aatatgattt tccaagtatt
     4021 aaatttgata taacaaaatt tgattttgac ataccaaatt ttgatataca tgaaatcata
     4081 aaaaaattta ataagtagta ataagtgaat aaggtttatt gattaaaata agccttattt
     4141 tagttatata tttttaatgt ataaaataaa aattcattaa ctgtatttta taggatttac
     4201 tttaagttta tgatattata taatatttag tgttagactt gataatccac atataatgtg
     4261 gataattttg tttagtgcca attagtttat attcaaaaat tttaaatttg gtatataaag
     4321 agtttttaga taatgtaaaa taataactat aagaataatc aagaggaggg aattttaagg
     4381 tggacaataa acaaagtaga actagaaatt ttagtataat tgcacatata gaccatggaa
     4441 agtctacctt agctgataga ttaatacaac aaactgggct tgttagtgaa agagacatga
     4501 aaagtcaatt acttgacaat atggaccttg aaagagaaag aggaatcact ataaagcttc
     4561 aaaacataag attaatgtat aaagcgaagg atggaaatga atattattta aatcttatag
     4621 atacacctgg acatgtagac tttaactatg aggtatcaag aagtttagct gcatgtgaag
     4681 gagcactatt agtagttgat gcagctcaag gtgttgaggc acaaacttta gccaatgttt
     4741 atcttgctat agaccaagat ttagaaatat taccaataat aaataaaata gatttaccaa
     4801 gtgcaagacc agaggaagta aaaaatgaaa tagaagattt aatagggctt gattcaagtg
     4861 aagctcctct tatttcagct aaaactggtt taaacataga agatgtttta gaagatattg
     4921 taaaaaatgt tcctccacct aaaggagata atgaagctcc attaaaggca ttaatattcg
     4981 attcttacta tgatgcttac aaaggtgttg tagcttatgt tagagtattt gaaggaactg
     5041 ttaaaaaggg tatgacaata aaaatgatga ataccaataa aaagtttgag gtaactgaag
     5101 ttggtgttat ggctccaggt caaactgaac ttagtgaatt atcagcaggt gatgttggtt
     5161 atattgctgc tagtataaaa gatataagaa gttgtcgtgt tggtgataca ataacagatt
     5221 caaataatcc tacagaagaa cctttaccag gatacaaaaa agctactcca atggtatatt
     5281 gtggtatata tccaggagaa ggagaaaagt atgagaatgt tagagatgct cttgaaaagc
     5341 ttcaagtaaa tgatgctgca cttgaatatg aagctgagac ttcagcagca ctaggatttg
     5401 gttttagatg tggtttctta ggtcttttac atatggagat aatgcaagaa agacttgaga
     5461 gagaatttaa ccttgatata attactacag ctccatctgt tatatataga gttacaaaaa
     5521 tggatggaga ggttgtaatg atacaaaatc ctgcaaactt accagaacca tctgagataa
     5581 agatgataga agagcctata gtaaaaggag atataatagt tcctaaagac tatgtgggtg
     5641 ttgttatgga gctttgccag gaaagacgtg gaaatatgtt aaatatggaa tatatagatg
     5701 atagaagagt aatgcttcac tatgaccttc ctctcaatga agttgtttac gatttctttg
     5761 atgctttaaa atcaagaact agaggttatg gttctcttga ttatgaggtt aaaggttatg
     5821 tagcatctac acttgtaaaa cttgatatat taatcaataa agagcaagtg gatgcactta
     5881 gttttattgt ccatgagaca cgtgctttcc ctagaggaaa ggcaatgtgt gagaaattaa
     5941 aaggagaaat tccaagacat cagtttgcta taccaataca agctgctgtt ggtaataagg
     6001 ttattgcaag agaaacaata agcgctctaa gaaaagatgt tcttgctaaa tgttatggtg
     6061 gagatatatc tcgtaagaag aaacttcttg aaaaacaaaa agaaggtaag aagcgtatga
     6121 gacaaattgg ttctgttgaa gttcctcaaa aagcatttat gtctgtgctt aaattagatg
     6181 agtaaatcct tataaattta gataaagata tttaaaagct agagtgattt tagaattatt
     6241 tctaaattag tctagctttt ttatggtata ttttaagtaa actcatagag aatataagta
     6301 agaatattaa agaagaataa aaaggtgata tttatgatta atatagaaat tcctaaaaaa
     6361 gtagactata taattaaaga attagaaaag aatggttatg aagcttatat agttggagga
     6421 tgtgttagag attgtttatt agaaagagtt ccaaacgatt gggatataac aactagtgct
     6481 agaccagaag tggtagtaga attatttgaa aaaacaattc caactggtat acaacatggg
     6541 actgtaacag ttatgattga acatgaacca tttgaagtga cgacttatag aattgatgga
     6601 aattatagtg atggtagaca tccagattca atagaattta caaataacat agttaaggac
     6661 ttgtcaagaa gagattttac tatcaattca attgcataca attcaaagac aggattagtc
     6721 gacccattta atggctatga agatatacaa aataagtata tacgttgtgt tggaaatcca
     6781 gtagatagat ttgaagaaga tgcacttaga atgcttagag cagttagatt ttcagcacaa
     6841 ctcaatttta aaatagctga aggtactaag caaagtattc ataaaaaagc agatttgata
     6901 aaaaatgttt ctattgaaag aatccagaca gagtttaata aaatattagt atctgattca
     6961 tctaaattaa acttattaaa atcaacaggt ttgttgaaat ttataattcc tgaaatatgt
     7021 gaattagaag atgttactca acacaatcca tatcacatct atgatgtaca aaaacatact
     7081 ttaatagcta cagaagtaat tgaagatgaa ctgtatttaa aacttacaat gttgtttcat
     7141 gatttaggta aaaaagtaac taaaacaact gataaaaatg gtgttgacca tttttatact
     7201 catagtagag aatctgtaaa gattgcaaag aagattttga aaagacttaa gtatgataat
     7261 tatactatta ataaagtttt aatattaata caatatcatg attatagaat agaacctaaa
     7321 aggaagataa ttaaaaagtt attaaataaa cttgaagatg ttgaattatt tgaagattta
     7381 ataaaagtta attgggctga tacacttgca aaaaatccaa agtatgcaaa acaaaagata
     7441 ttaaatctta ttgagtgtga aaaagaattt aaacatataa taaatcaaaa ggagtgcttt
     7501 aatcttaaag atttagctat taatggaaaa gatttgatat ctataggtat aaaaccaggt
     7561 aaagatatag gatatatttt aaataagatg cttgaaatag tcataaataa tccagagtta
     7621 aatgaaaaag aaatactaaa agaaaaagca ttaaatatat atacatttta gattcaaata
     7681 taaaaaattt aattacttta aaaaacatag aataaaatac ttatttagat aaaataaata
     7741 atttttagag aatattttag aagttacgat tgtatgattt ttaaatgcat aggcatttac
     7801 tctaggacta tagactaata taaaaaaaaa ctttgggaat actaaaacca taaatttata
     7861 aaattaatat tttatatact gtaaaacagt gaggagggac agtatgcata ctcaactaca
     7921 aaagactatt ggtctttcag cagctctttc tactgttgta ggtatggtta taggttcggg
     7981 agtttttttt aagccacaag caatatacac tacaactaat ggagctccag gtctaggtat
     8041 tatagcgtgg cttttaggtg gttttataac aataactgct gggcttacag ctacagaaat
     8101 ttcagctgct ataccaaaaa ctggtggtat gatgatttat atcgaagaaa tatatggaga
     8161 aaagttaggt tttttaactg gatggatgca aactgtatta tttttcccag gtacatcagc
     8221 ggcattagga gtaatatttg ctcaacaagc atctgaactt ttaggaatga gcccaaataa
     8281 tatggcaaat gtacttccta tagcaattgg agttatacta tttttagcgt tattaaatat
     8341 aataggttca tccttaggag gaaaagttca gacagtagcc actattggaa agatgatacc
     8401 tttaatttta ataatagtct ttggatttat taaaggtcaa agtagtgaag tattaaatcc
     8461 atttgtagga gatggtgtaa atgcatcaaa tgcacttgga caagtattaa tagctacttt
     8521 gtttgcatat gatggttgga taaatgttgg agctatatca ggagaaatga agtctccaga
     8581 gaaagattta ccaagagcaa tagttggtgg tttatcatta gttatggcag tttatattat
     8641 aataaatata gcttatcttt gggtagtacc agcaagtgaa ttagcaacag ttacttcccc
     8701 agctacatta gtagcaacaa gattgtttgg aaatataggt ggaaaagtaa taactgtggg
     8761 tatattaata tcagtatttg ggacattgaa tggatattta ttaacaggtt caaggatacc
     8821 ttatacatta gctgaaatgg gaactttacc agcatctaaa actcttttaa aagtaaattc
     8881 tggaggttct cctgtaaatt caatactact tataactgtg ttagcctgtg tatatgcatt
     8941 atctggtcaa tttaatcttt tgacagactt aacaatattt tcaatatggg tattttatgt
     9001 attgactttt ataggtgtta tgaggcttag aagacaaaag ccagatttac acagaccata
     9061 taaagtccct ttatatccaa taataccaat tatagctata ttaggtggac tgtttgtaat
     9121 aataaatcaa atattgactt caacagtcat atctttaggc ggaatattta taacactatt
     9181 aggtttacca gtttattatt atatgaaaaa aagataacta cttaatttaa gttagataaa
     9241 tttgggtaaa ggtgaaaatt aacttttatc caaatttttt atactgttat acaatataag
     9301 tattatatat tgcttgatgt gctattttgt ttgatttata atttagtaat tgatgtttta
     9361 agataattta agaaatgagg agtgaaagat gctaggactt tatgtacata ttccattttg
     9421 tgtgaaaaaa tgtaagtatt gtgattttaa ttcttataaa atggacatag attcaaaaaa
     9481 aagatatata gaagacttaa aaatagaaat ggaattatat agtaataaat tatataaaga
     9541 taataaatat aaaaataaag aatgctgtag tctaaataaa aatgacaaaa taacgagtat
     9601 atttgtaggg ggaggtacac ctagtatact aacctctgat gaaataagag aagtatttat
     9661 aagtataaaa gaaatgtttg atatagatga gaatgcagaa ataactatag agtgtaatcc
     9721 tgggacatta acattagaaa aattaaaaac tatgaaagag attggcataa atagacttag
     9781 tatagggcta caagctattc aagaaaagca tttgaacttt ataggaagaa tacatactta
     9841 tgaagaattt gagaaaaact ataaagatgc attaagtgta ggttttaaaa atattaatat
     9901 agatttaatg tacagtcttc caaatcaaac tctatgtgat tggaaggaaa ctcttgaaaa
     9961 agttgtacac ttaaatccaa cacatatatc agcttattcc cttatactag aagaaggaac
    10021 tgaactatac aatatgtatg aaagtaataa atttgaactt atagatgaaa atgtagatat
    10081 agaaatgtat gagtatacaa taaactattt aaagtcaaaa ggatataatc aatatgaaat
    10141 atcaaactac tcaaaagaag gctataattg tgagcataat attttatatt gggaatgtga
    10201 acattatata gggattggag caggagcttc tggctatatt aatgagaaca gatacaataa
    10261 tgtagaatct ttagaagatt atcatttaag tttggtaaaa agagaaaaac caatacaaga
    10321 aaatgaaatt ctttctgaga aagatatgat agaagaaaag atatttatgg ggcttagaat
    10381 gaataaaggc ataaaatttg aagattttaa aaaaaaattt ggaatagatt ttagagagaa
    10441 gtataataag caaatagaaa tgctattagc tagaaagctt ataaatcaga gctttgaagg
    10501 tatacaatta actcaaaaag gaagagaaat atctaatagt gtatttatag aatttatgga
    10561 ataattttta aggtgttgac aaaataaatt tataatggta tataatatat ataatctttt
    10621 tagcactcga acttagtgag tgctaacagc taggaggtat aataatatgg aattaaatga
    10681 aagaaaatta aatatcctca aagctatagt taaagattat atagaaacag ctgaagctat
    10741 aggttctaga acaatatcta aaagacatga tttaggtgtt agtgctgcta ccataagaaa
    10801 tgaaatggct gaccttgaag aattagggta tttaattcag cctcatactt ctgcgggtag
    10861 agttccatct gaaaaaggct ataagttata tgtaaactct cttatgagta aaagcgaatt
    10921 ggatgataat gataagattc ttattgaaca atgtatgaat cataatataa atcatataaa
    10981 agaattgatt catgaaactt ctaagctatt atcgcaacta acaaactata caactgtagc
    11041 agttactaag agtttaataa atcaaagtgt tataaagcac atacagttgg ttgctatgaa
    11101 tgacaataat attttactta tagttgtaac agataaaggg gacttaaaaa aagctaacct
    11161 tacaactaat gtatatttag atcaatctaa gttaaatttg atttctgata atcttacaag
    11221 gaagctctta ggtaaaagta taacagattt ggatgataat ctaattgctt ttattaaata
    11281 cgaaataagt gagtattctg ggcttataga tgagctttta aatgctttga actcaaatat
    11341 gaaagaagaa gatttttctc tatcattaaa tggagctact aacatattta gttatccaga
    11401 atttaatgat gtattaaaag caaagtcatt tttaaatatg ttggaaaaaa aggaaacaat
    11461 agctgacata attaaatcaa agggaataca aaaagacaat ctaaatataa taattggtag
    11521 tgataatgat tgtgaactgg cacaggattg tagtatagtt acagcaactt ataatgtaga
    11581 tagagacttg gttgggcgaa taagctttat aggccctacc agaatggatt atgctagaat
    11641 ttactctata ataaattata tgagtttatt aattaataga aagtaaagaa attttaaatt
    11701 gcgaggtgta aagtcttgga taaaaaaaat gagcaacagg aagtaagaga agaaaatgat
    11761 acttctatta accaagaatc ggaaactcaa gtagagttag aagaggaagt tgttaatgaa
    11821 gaatgtgaaa catcaagtga aaaaactgat gaaaaagaag ttgatgatga aaatgtaaca
    11881 gatataaatt ctaagttagc agaaaaaaaa ttgcaggatg agttggatga attaaatgat
    11941 aaatatcaaa gacttcaagc cgaatatgca aactacagaa gaagaactca acaagagaaa
    12001 gagacaatag gcgtatttgc aaatgaaaaa ataataactg aattgatacc agttatagat
    12061 agtatggaaa gagccttaga tgcatgtgaa gataaggaag atactatgta taagggtata
    12121 agccttgtac ataaacaatt aatagacact ttagtaaaat ttggagtaga agaaatagaa
    12181 gcagaatcta aggaatttga ccctaatcta catctagcag ttatgcaaga aagtgtagat
    12241 ggagttgaag caaatcaaat agtaatggta cttcaaaaag gatataagct aggaactaaa
    12301 gttgtaagac cttcaatggt taaagtttct tgctaagtat aaattaaagg ttaaattatt
    12361 taacctaata taaaatatta aaataagtaa aacttaatta tataattact cgaggaggat
    12421 tttagtcatg ggaaaaataa taggaataga tttaggaaca acaaattcat gtgttgcagt
    12481 gttagaaggt ggagaagcac aaataatagc aaatagtgaa ggtatgagaa ctactccatc
    12541 agtagtagca tttacaaaag atggtgaaag aatagttgga gaacctgcaa aaagacaagc
    12601 agttacaaat gcagataaaa caataacttc tataaaaact catatgggaa ctgattataa
    12661 agtaaatata gatggaaaat catatactcc acaagaaata tcagcaataa tattacaaaa
    12721 attaaaatca gatgcagaaa gttacttagg acaaacagta acagaagcag ttataacagt
    12781 accagcttac tttacagatg ctcaaagaca agcaactaaa gatgctggta gaatagcagg
    12841 gcttgatgtt aagagaataa taaatgagcc aacagcagca gctcttgctt atggtatgga
    12901 taaattagac caagaaaaga aaatattagt atttgactta ggtggaggaa cttttgacgt
    12961 atctatactt gaaataggag atggaacttt tgaagtttta gctacagctg gtaacaatag
    13021 attaggtgga gatgactttg accaaatagt aatagattat ttagcagagg aatttaaaaa
    13081 agctgaaggt gtagatttaa gaaatgataa aatggctctt caaagattaa aagaagcagc
    13141 agaaaaggct aagaaagaat tatcatcaac aatgagttca aacataaact tacctttcat
    13201 aactgctaca gcagaaggac caaaacactt aaatatagat ttatcaagag ctaaatttga
    13261 agaattaact agaggtttag ttgaaaaaac tatggaacca actaagacag ctttacaaga
    13321 tgcaggactt tctacaggtg atatagatga tgtattatta gtaggtggtt ctacaagaat
    13381 accagcagta caagaagctg ttaagaagtt tataggaaaa gaacctcata aaggtataaa
    13441 cccagatgag tgtgttgcag caggtgcttc tatacaagct ggagttttag caggggatgt
    13501 taaagactta ttattactag atgttactcc attatctctt ggtatagaaa ctatgggtaa
    13561 tgtaatgact aagataatag aaagaaatac tacaatacca actaagaagt ctcaaatatt
    13621 ctcaactgct gctgataacc aaacagctgt tgatatacat gttcttcaag gtgaaagaag
    13681 tatggcttat gacaatacaa ctttaggaag attccaatta acagatatac caccagcaca
    13741 aagaggaata cctcaaatag aagttacttt tgatatagat gcaaatggta tagttaatgt
    13801 ttcagctaaa gatttaggta caggaaaaga gcaaaaaata actataacat caaacactaa
    13861 tttatcagaa gcagaaatag agcaaaagat aaaagaagca gaaatgaatg cagaagctga
    13921 caaacaaaag aaagaaaaaa tagaagcatt caatcaagct gaatctacta tatatcaaac
    13981 tgaaaaaact ttaaatgaac ttggagataa aataagttca ggtgaaaaag aggatataga
    14041 aaaagctata gcagacttaa aagctgtaaa agataatcaa gatgctacag cagaagaact
    14101 taagaaagct acagatgaag taatgactaa gttccaaaaa gtatctcaag aaatgtacca
    14161 aaaagcagct caagaacaac aagcggcaca aggtgcagaa caagcacaag acaatggacc
    14221 taaagatgac aatgtagtag atgctgactt taaagaagta gatgaagata aataagctat
    14281 ataatatgta acactaaatg ataattggaa catatatgat ttgtgaaaat catatagatg
    14341 ttaaagatat atggacaata tacatcataa agaatataat atatcataga ttatataact
    14401 taaagtagaa tataagaatt aatgatagct tgtagtttat tattctacaa gctatcgttt
    14461 tgtaaaatga aggtggtgtc aatttttgag tacaaaaagg gattattatg aagtcttagg
    14521 tatcagtaaa ggtgcagaag ctcaagagat aaaaaaagct tatagaaaat tagcaatgaa
    14581 gtaccatcca gatagaaatc ctggagataa agaggcggaa gaaaaattta aagagataaa
    14641 tgaagcttat gaagttctat ctgatgatac aaagagaaaa acttatgacc agtttggaca
    14701 tgatgggcta aatggtcaag gcggttttgg tggtcaagga ggatttggcg gtcaaggctt
    14761 tggtggtttt gaagatatgt tcggagatat atttggtgac atgtttggag gaagcttcgg
    14821 aggtggaaga gcaagaagaa gagggcctca acgtggtgca gatataagac agagtgttac
    14881 tatatctttt gaagaagctg catttggtaa aaagatgtct ataaaagtaa atagaagtga
    14941 agaatgtgaa gaatgtaatg gaactggagc aaaaccagga acatcaaaga aaacttgttc
    15001 aacttgtaat ggaactggtc aagttagaac tgttcaaaga actccatttg gtaatatagc
    15061 aagttcaaga ccatgtagtg cttgtaatgg aactggagaa gtgattgaat ctccatgtag
    15121 taaatgtcat ggaactggaa atacaagaaa agttaagact atagaagtag atatacctgc
    15181 tggtatagat gatggtcaaa tgataaaact ttctggtcaa ggtgaagtag gagaaaaagg
    15241 tgctccaaga ggagacttat atattgtagt taatgttaag tcacatccac tatttactag
    15301 agatggaaat gatatttatt ttgaaatgcc aattacattt gttcaagcaa ctttaggtga
    15361 tgagatagaa gtacctactt tggatggaaa agttaaatat agtgtgccag aggggacaca
    15421 aactggaact gtatttagac ttaaagagaa aggaatccct agaattagag gtaattcaag
    15481 aggagaccaa tatgttaagg ttgtagttga aattcctaaa aaattaaacg ataagcaaaa
    15541 agaactattg agagaatttg cgaaggagtg tgggtcgaac gtccacgaaa agaagaaaac
    15601 cttcggacaa aagatagaag atatgtttaa gaaaaagtag ttaaagtagc caatatataa
    15661 tcatattttt aaaatcttag ttatttataa gattgtcaaa ggagatatta tgatttttat
    15721 attggctatt tttgtataaa aaataaatgt gcataaaata aaactgaata taaataattt
    15781 tctgaaaaat acttgtccta aaatatataa tatgctataa tataatcaaa taaaagaaag
    15841 gggtgattcc agtgggaacg gatgtggcac tgccaaatga tgaagaaaac tttattgtaa
    15901 tatacaaaat aaaattagat acttttagtg ctagattaag tactggaaaa actcatgagc
    15961 tgggataatt attgtaatta cctagactca aacttttagt agtaaaatag taatattata
    16021 tagacaaggt atctaatttt taaatctttt gtatgaatta aaatttttat aaagggtata
    16081 tctgattaga atgtaattag ggaaacctag ttgtgaatta cttttagtat atacatgtgt
    16141 aaagaaatat gtatatggta ttggttattg tgctagatgt gtatgtaaag gaatatgtgt
    16201 ctagttgggt atatgtaaag aaatatactt ataatctatg aaaaattttt agtataaatt
    16261 gctaaaacat tgagattgtt ttttatagaa agttagttta taccaactaa aaatgtgctt
    16321 aagatttgtt tgatgtttaa ttataaataa ggttgtagat aaaaaaataa gtaaagacaa
    16381 aggtttaaaa tacttgcttt aaatatagtt tgttttgaac acttgccaaa ttaaaataat
    16441 taataaaata gacatcttct catagtaaaa tatactgtga gaggatgttt ttgctttgtt
    16501 cataaacttt tatgaaaaat ttgcatagtg tcacatttta atatactata tatataataa
    16561 aatatagata aaattaaatg attcaaaaga gaaaggtgaa aagagatgta ttatataggt
    16621 gtagacatag gaggaacagg tatacaagca ggagtagttg ataactatgg gaaaataatt
    16681 tttaggtcag agtgtaaaac agtaattgaa aaaggctttg aaggaatact taatgacata
    16741 aaaataatga tttataagtt gttggaagat aataaactta ctatgagtga cataaaatct
    16801 attggatttg gtgttccagg ttttataaat aaagaaggat tagtaacttg tgtaaattta
    16861 aaatggaata aaaaagcttt taataaagag ttaaaaagaa gatttccaga tgttgaaatt
    16921 catggagaaa atgatgcaac agtagcagca cttggagaag caaagtttgg aagtatgaaa
    16981 ggtgctaatg ttggagtatt atatacttta ggaactggag taggtggagg aatagtaatt
    17041 aatcaaaaag tcttttctgg agcacatgga ttgggttcag aaataggtca tcaaattata
    17101 ggagaaaact attttaattg taattgtgga aataatggtt gtgtagaaac tttttgttca
    17161 gcaactgcaa ttataaagta ttctcaaaaa ctgatagaag aaggagaaaa gagtaggatt
    17221 ttagatttgg cagagggaaa tttagaaaat gtaaatgcaa agatggtgtt tgatgcatat
    17281 agagaaaatg atttagtggc aattaaagta ataaatagat ttaaagagta tttagcaaaa
    17341 acttttgcaa acacaataaa ttctttagac ccagaaatta tatctatagg aggaggcatt
    17401 tctaaatcta gcgatataat acttgatgga atagaagatt tggtaagaaa atttgtatta
    17461 tacaaaacag aagatatagc tacaattaca tgtgcaactt taggttcaga cgcaggaatt
    17521 ataggagcag cttttttata atgtaaatga actgcttatt ggaggcgtga tgatatatgg
    17581 gtaaaacaaa tgctagaaga acgctttttt tgatagcaat aggttcagga actatgctga
    17641 atcctcttaa ttcttctatg atttcacttg ctttacatag tatacaaaat gattttcata
    17701 ttagttttac aactgtatct tggattgttt cagcatttta cctagctagt gctattgcac
    17761 aaccagtttc aggtaagata ggagatttaa taggtagaaa ggtcttattt ttgagtggtt
    17821 tgatgcttgt aattttatca gcatccatag taccacttgt tcaatcattc tttatattga
    17881 tttttataag agtaattcaa gcaattggaa caagtaccct atatccatct ggagttgctc
    17941 ttatacaaaa taatataaaa gaaagacaat cttctgcatt agcagttcta accatttttg
    18001 cttcaactac agcaggatta ggtccgacac ttggaggatt attgttggac ttaggaggat
    18061 ggcatgctat atttcttgta aatatacctg ttgttttggt ttctatatgc ttagggtatt
    18121 ttttattccc aaaagaagta aaagaaaaga aatctataaa agaaacttta aaaaatgttt
    18181 tgtcaagatt agatattatt ggcatacttc tattttcaat tgcaatgatt tttatattac
    18241 tatttttgct atctatgaag gagtctttta acttggaaca acttgtattt ggtataatac
    18301 tgatgtgtct ttttatttgg catgaattga gaacaaaatt gccatttata gatattaagc
    18361 tttttgtaag taatcctaaa ttgtcaaaag tttacttgca atttattata ttaaatttct
    18421 ttaattatat tttgtttttt ggtcttccta gttattttca agatgcctta cattatagtg
    18481 caaagtcgac tggattattt atgctattta tgtctggtat aggtatattt atatctccat
    18541 taactggaaa atggatagat aaatctggaa ctagatttcc tcttgttaca tcatcaatct
    18601 ttatgtttat tagtgcagta cttctttcag tagtttttat ccatatacct gtaataggaa
    18661 agggtattat attatcatta gcaggtatta gttatggagt tggtaatgta gccctgcaat
    18721 cctctatgtt tgaagaaagt cctcgtgaca gtatagggac agcttctggt ctgtttcaaa
    18781 ctagtcgtta tttaggttct attttagcaa ctgttttact tggaatggta tttgaagtaa
    18841 atataacatc agaacaattt cagatattag gttatgttat ggtggtctta ggtacaataa
    18901 gttttttatt aaattttata ttaaagaaag aattaagaaa agtagaatag tggatttatg
    18961 gatttaataa gtctaataac tgataagttt aaaaatattg tttgtaaaac ataactggtt
    19021 tgatatttat taattctaag ttttgaagca tagagtactc gataagtata caatattttg
    19081 aattaaaatt aagcagaaat ttgaactagt ctttcaaatc tctgcttaat ttagtagtta
    19141 aattttttta tagagaaaat aagatttgac acttagataa aaaatcattg cattttcaaa
    19201 attctttaaa tcaaacccaa gtatctcatt aatcttttcc aatctatata ctagcgtgtt
    19261 tctatgtaaa aacatactag tagatgtctt agaaatattt aaatcattat ttataaattt
    19321 tattcctgta tatagaagtt cttcgtcaat agatttaatc ttattaaaat ctattaattt
    19381 attgagtata ttatcctcat taattaaatc taaagtttta tatctaataa agtcaaacaa
    19441 atttaaaaac ttattttcag cgttgtcttt tataatatga ggtagtaaat cagaacataa
    19501 ctgtaatcta aaatcaaaat cag
//
LOCUS       DAELTA010000071        19441 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.071, whole genome shotgun sequence.
ACCESSION   DAELTA010000071 DAELTA010000000
VERSION     DAELTA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.071"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(101..547)
                     /locus_tag="KPG13_002074"
     CDS             complement(101..547)
                     /locus_tag="KPG13_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985790.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
     gene            complement(1065..1745)
                     /locus_tag="KPG13_002075"
     CDS             complement(1065..1745)
                     /locus_tag="KPG13_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985791.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(1795..3810)
                     /locus_tag="KPG13_002076"
     CDS             complement(1795..3810)
                     /locus_tag="KPG13_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0985792.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            complement(3882..4559)
                     /locus_tag="KPG13_002077"
     CDS             complement(3882..4559)
                     /locus_tag="KPG13_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985793.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(5139..5222)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(5455..6297)
                     /locus_tag="KPG13_002078"
     CDS             complement(5455..6297)
                     /locus_tag="KPG13_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0985794.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(6549..8333)
                     /gene="pepF"
                     /locus_tag="KPG13_002079"
     CDS             complement(6549..8333)
                     /gene="pepF"
                     /locus_tag="KPG13_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG0985795.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(8399..9064)
                     /locus_tag="KPG13_002080"
     CDS             complement(8399..9064)
                     /locus_tag="KPG13_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG0985796.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(9215..9376)
                     /locus_tag="KPG13_002081"
     CDS             complement(9215..9376)
                     /locus_tag="KPG13_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG0985797.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(9454..9963)
                     /locus_tag="KPG13_002082"
     CDS             complement(9454..9963)
                     /locus_tag="KPG13_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG0985798.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            10169..10387
                     /locus_tag="KPG13_002083"
     CDS             10169..10387
                     /locus_tag="KPG13_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0985799.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            10405..12351
                     /gene="feoB"
                     /locus_tag="KPG13_002084"
     CDS             10405..12351
                     /gene="feoB"
                     /locus_tag="KPG13_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0985800.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(12530..13585)
                     /locus_tag="KPG13_002085"
     CDS             complement(12530..13585)
                     /locus_tag="KPG13_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0985801.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(13633..14433)
                     /locus_tag="KPG13_002086"
     CDS             complement(13633..14433)
                     /locus_tag="KPG13_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0985802.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(14426..15217)
                     /locus_tag="KPG13_002087"
     CDS             complement(14426..15217)
                     /locus_tag="KPG13_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0985803.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(15257..15679)
                     /locus_tag="KPG13_002088"
     CDS             complement(15257..15679)
                     /locus_tag="KPG13_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG0985804.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(15695..16174)
                     /locus_tag="KPG13_002089"
     CDS             complement(15695..16174)
                     /locus_tag="KPG13_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985805.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(16306..19062)
                     /locus_tag="KPG13_002090"
     CDS             complement(16306..19062)
                     /locus_tag="KPG13_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985806.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6454 a         3281 c         2141 g         7565 t
ORIGIN      
        1 tatatctgct attaagattt gttaactaaa tttaactttt atactacata tataattatt
       61 gtatcctcta gtttatatca ctattatttc catcaaattg ttacgatact tgatgtttgc
      121 aataggtatg aatagccaaa aatataaaaa taggtatttc tatcactgag aaaaacagta
      181 taatgtgtgg tgtccaaaaa ctcatacctc ctaagtgtaa gaaattaaat gatgtcattt
      241 gatataataa actaatctta aaaccaattc cccctggcgg taataggtat ccaatccaac
      301 taacacctat tcccatagga attaagcaaa tagaaaaagc aatcagcata gaagtaagag
      361 aatccttaca ttttgcagat aaaaataatg ttaaactaac cattgcaagt attgaaatta
      421 gaccaccaat agctataaga atctgtgctt gtagtaaatt tatatttact agactaatga
      481 cagaaaacaa catttgaaat gaggttttca aacattcagt accaaaagct aaatcagaaa
      541 ttgccaaata gatagaaata catataacaa acattattat aaatatagaa aatagagcta
      601 atattttagt aaacgctaga caaatatgtc catgtttcgt acaacgaagg atgctatctg
      661 attgtgtctg atactcacca gaaaaagtag gtgcagcaat tgcaacatat ataattaata
      721 atataaaaat ataaaatatt atataatcga aagcatcttc tggatttatt aagaaattct
      781 taatcttatt atttaactat tttattattt aatatatatt aaaggtaaaa aaataatata
      841 tagatttaga tatacaattt atctatcttt tcaaatataa tctataaaat aatagttcca
      901 ttacgcattt ttttatataa taaaactgac tgtaatttaa ctggcaaagt ctagaagaat
      961 aggctttatc aattaaatta cagtcagcta ttttataata atttagttta gatattgtta
     1021 gtatttcaca tttataacaa ttattaatga tattaatata tttattattc tttattaaaa
     1081 taatatcctg ctcctcttat tgttataatt ttctgaggat ttttataatc tttctctatt
     1141 ttctttctta atccattcac tgcaactacc acactatagt tatcctctaa gtattcattc
     1201 atccatacat gttcatatat ctctttagta gtcaaagtct tttcaggatt ctctataaaa
     1261 aagtttaaca acttattttc tgttggagtc aattttatta ttttatcttc taactttatt
     1321 actccactag ttctattata ttcctctaat tttgtattat tttcattatt atttacatat
     1381 gagttcattc ttcttaaaat agcttttact tttgcgtata atatttctaa atttagaggc
     1441 tttattaagt aatcgtctcc accagaattt aaagcattta aaatgctttg gtcttcattt
     1501 atataagtca tataaacaat tggacaagta gttacatttc taattttttt acaaagagtc
     1561 catccatcac catcacttag aattatttct aaaaatatta aatcatattt attgctaaat
     1621 atttttccaa tagcttcttc acaattatat gctaaatcta tacttattcc tttttcctcc
     1681 agaaatcctt ttagcttctt gcaattatcc ttgtctccat ctattatcaa tatcttagtt
     1741 tgcattataa tcactcccaa atatcattat tcttttatat taaataagct attattataa
     1801 aaaatataga cttaatttta ttgtaaaaac agttcctttt cctagttctg aatctacaaa
     1861 gatttctcca ccatgctctc ttataatgga tttgacaata tatagcccta acccttgccc
     1921 ttttggctca ttattgtctc tttctgtaaa gaatctgtca aatacctttg gtatattttc
     1981 tggaggtata ccttttccat tatcagaaat tttaataatt gcaaatccat cttctttatc
     2041 cattgataaa attatctttc catcaaatgg agtaaaggtc aaagcattat aaattaaatt
     2101 ctcaaatgct cttgtaagca tctctttatt tatctttgca tatatttttt tctccaatac
     2161 gtaagtttca aagtttacac cacatgcttg tgtatctggc aaattatttt catatatctc
     2221 tttaataaaa ttattcagac atattttttc actaatacca atgcttttat cttgtgatgt
     2281 aaattcttgc aaactacgta ctctattttg tacttcattt gacttttgat ttatagcata
     2341 aagataggtt cttgtctctt catctacatg aatattacca acctttatca aatctataaa
     2401 tgcttgtata gccgaaacag gtgcctttaa atcatgtgct acatctgcca agaatctttt
     2461 acgttcattt aacaatgttg taagttcatt agttctcact tcaacttcat gttcaagatt
     2521 ccttgtaagt ttttcattct cttttattat tattctattc ctgtttatca tcataacaga
     2581 aaataaaata acaattacaa acccacaata ttctgtttgc caccctgtaa atataggctc
     2641 aaatctatta ctcgtaactg catcaaataa tatgctaatt ccaaaaacag tgtttactga
     2701 aactaaaata taagaactta tatgctcaga gcatgtcttt ttatatattg aaaccagcga
     2761 agacattaat atatatataa atacaatata tttatatatg tctattaact tcccatatat
     2821 gttgacaaag tacatatcat atggaagaag aataattggt atcactatag atatgatact
     2881 cattgtaatt cctaatggta aaattaaaaa tctgtacact ttattttctt ctattttgga
     2941 tatacttcca tttatacaaa taatacataa aactaccata aaatatgaca aatcttcaac
     3001 cgcatatgta aagcttacca aaggcagtcc taacaaatgt acaaagggat aggatacatg
     3061 gatggcaaag aaaacacaca tacatccaaa atataagtaa attttacttt tgtttgaaag
     3121 aaaccataca gacaatgaaa atatagctat tgcaaaagtt gtgaagcaaa ttatactata
     3181 aaaaagtaat cgataaaaca tcatattact tatgtccttt gtttctccta atgcaggcgg
     3241 ataatacaaa ccactataat aatgtgaaaa attcgataca ttgattataa tttctgtatt
     3301 tttctttgca ataaaactta ctactgaatt tttaattttg ggtctatatt tttctgttcc
     3361 tacatctcct aatttagaaa ccatttctcc atttatccat acttcacttg cggaaaaaat
     3421 ctcaggaagc tctaaactta gtagcttatc cttgccttca ttttttattc tcatacgata
     3481 agtagcattc ccatatggtg actgagtccc ctttctcata gaaaaatctg gatattgacc
     3541 tataaaaaca tattctggtt ttatatcttg ctctgtaaaa tcattcggtt taaatatttt
     3601 atgattataa aactcccagt catctatcaa aaagacaagt ttacctttag tgaaagtatc
     3661 attatctata gttaaaattc cattacttgc tctaatagac ttatatgtat atttattatc
     3721 atatttatac agaagattta atcctgttat tgccaacaga gagactatca atacaatgag
     3781 agttcctttt agtatattat actttttcat tgtatcaacc acatctcttt catagtagta
     3841 ttaaataaaa caagaactat ttattaatta cagtatctca cttatggtac aaacttatat
     3901 ccttttcccc atacggtttg aatgtattga tgctccctgc ttaaagaatt aatttttttt
     3961 ctcatctgtg caatatgaac ctgcactgta tgttgatctc caatatcagg cattttccaa
     4021 acttggttat atatctctat cttcgaaaat acttgttctt tgttttcagc tagaaacaat
     4081 aatatttcaa attccatagt agttaaatct atactttctc catcaaaagt tacttttctt
     4141 gaatttacat ttattactaa cgctgaaaac tttaacacat taggaactga tgtaatattt
     4201 ttgtactgcc tcatacgagc atatattcta agttcaagtt cttttaaact ataaggtttt
     4261 gttatataat catccccccc agctaaaaat ccccgaactt tttcttcttc atgctcataa
     4321 ttagataaaa atattatagg tatgttaacc ttttctttta tatcattgca cattttatat
     4381 ccatctatat ctggcaatga aatatccaaa attatacagt ctaaaactat actctctact
     4441 atatccatag catctttagc agtctctgat gtatatacat taaaacctat attagaaaaa
     4501 taaatttgat ttatgtttaa aatatcaaca tcatcatcta caaccagtaa attatacatt
     4561 acaccactcc attcaaaggt attttaatta taattccaag ttttatagtt attacaaatt
     4621 ataatgtttt attttcatat ataactggta ttattatttt ataatatctt tacaaactag
     4681 actataatac ttaagtattt ttgaataatt cttgaatatt gtattaaaca ttaagattca
     4741 ttattaaata ctaattaaag tctccgattg ttggagaatg gagcttaaag tcattttcac
     4801 gtataaattt attatttttc tagaaacttg tctattggct agtttgagta ttgatttata
     4861 tgatttatat ggttataata taaaataaat tgtattattt tggtaaatat attgttacaa
     4921 aaggtttata ttctgcatct caatgtttat aaaaacttta ttttcttatt ataatactat
     4981 tattctttta aaaaaatttc ccttttccaa caaatggcta ctttaaaagc caaaatttta
     5041 cctatcaata aaaatcttaa cgactaatac tcttattttc aaattttgca acatatttgt
     5101 taattttaaa acaaaaaaag actatgcaat ataaaaatta tattcatagc cggttgcacc
     5161 actaactcca tatatctcta gatgcccaat aataagatat acttctcagt ttctaaaata
     5221 tttttattaa aaatctttta tatattttca taaatttctt ttttattcta acattgtaat
     5281 attattttgt caatatttcc atattcttag agataagata ctaaaatacc tattttatct
     5341 aaagatattt atatttctac attcaataat agacaatacc ctgcaagtta aagcatcatt
     5401 tttatgagct tatctcacag ggtattatat gtttacttta tattataact cttgttattg
     5461 attaacttta ataaatcctt tttctgaaac aagtgcttgt ccttcatcac ttaatatata
     5521 atctattaaa gctttacctt tttctgacaa accatcttct ttattaacta atataaatgg
     5581 tcttgatatt ggatatttat tagctattac atttgcatca gttgcttcaa catcatttat
     5641 ttttaaacta tttactttct catcaacata tccaaatgat atatatccta tagcattttc
     5701 atttccttca actgtagttt ttatatttcc tgaaccatca cttatttgtg catctttagt
     5761 tagttcttct gaatcaaacc ctacaatttc ttggaatcca tctctagttc ctgaaccttc
     5821 ttctcttgat actattacta ttggtgcatc atttcctcct acttctttcc agttagtaat
     5881 ctttcctgta tatatacctt taacttggtc cattgtcaga ttttttactg agtttgcagt
     5941 attagttatc aatgctatac catctattgc tattttagtt tcctttaatc cagcactttt
     6001 ttcttcatct tttaaatctc ttgaactcat tcctatttca gatgttcctt ctatagcatt
     6061 ttttatacct ccagaagaac caatctgttg tacttctaca ctaacacctt gatttttttc
     6121 ttgaaacttt tctccgatag tttccataag aggacctatt gaagttgaac ctgaaacaga
     6181 aactttgctt gaatctcctg aatcacctga acctcctgag ttagaactac atcctactgc
     6241 taaagctcca actaacattg tactagctaa aagaacgcct attttctttt taaacatttt
     6301 acttaccccc gcttatattt taattttttg ttgtcatttt tctatatttt aataatacag
     6361 attctttgtt aaatgaatat tagagcaggg ttaagtaaat gtaaatattg ttaaatacat
     6421 gtaaaaactt aattttatat gatttattct taattagatt aactttttaa agttacttta
     6481 ggcagatata aacataatac aactcatatt atcagaagtc ttcattgaag ctatcaacct
     6541 atcacatcct ataattcttt ttctagttta ttaactaatt cagcaaatac atttaaggct
     6601 tcctctatag attctttttt cgtcatatct acaccagcag attttaattg ttctagtggg
     6661 tattctgacc ctccactttt taagaagttt atatatctat ctactgcagt acttccttct
     6721 gaaagtatct gttgacttaa agcacttgca gcagaaaacc ctgtggcata cttatataca
     6781 tagaagtttg aataaaaatg aggtattctc gcccattcta ttcctatttg tttatctact
     6841 attgcagatt ttccgtaata cttttcattt aatttgtagt atatattagt aaattcatca
     6901 gcagtaagtg gttctccact ctctaccctt tgatgtacaa ttttttcaaa ttcagcaaac
     6961 atagtttgtc tatgaactgt agttctaaat tgttctaaat aataatttaa taagtatata
     7021 gtttcatcct tagacttaga attttctaat aaataattta taagaagtaa ttcatttaat
     7081 gtagatgcaa cttctgcaac gaatatctta tagtccgaat ataaaaatgg ttgacttgtt
     7141 cttgaataat gactgtgaac agagtgccct aattcatgaa ttaaagtgaa tagagaatta
     7201 aggtcatttt tatagctcat taatatataa ggatgtgaat cataacttcc ccaagaataa
     7261 gctcctcctt ttttaccttc attatcatat acatcaatcc acccttcatc aaaagcactt
     7321 tttatgacct tcaagtattc ttctcctaaa ggtttaagtg cctctaatat gatatcctgt
     7381 gctttatcat atggtatatc catgtcaaat ttatctgtca aaggaacata caaatcatac
     7441 atgtgtattt cattcaatcc taaaaatttc tttttaagct caacatattt atttagatta
     7501 ggaatacttt tatctatagc tgatataaga ttattgtaaa catctaaact tacatcatct
     7561 gaaaataaag acgcttctat tgctgaatta tactttctag tttttgcgta aaatatctct
     7621 gattttattc caccataaag cgttgaggca aatgtatttt tatatttctc atatgttgaa
     7681 aattcacctt caaaagcgtc ttttctaact ctactattct tactttttat aaataaagag
     7741 tagtttgaat gagaaagctt aaccttattc ccctcctcat cttctatctc aggaaattcc
     7801 atatctgcat atgctaacat atcgtataca ttttctggaa ttgtcgataa ttctgatgta
     7861 gctgcaagta tttcttcctc tcgttcactc aaagtatgtg gtttatctct caatatatcc
     7921 tttatatatt ttgcataaaa ggatagcttc tcatctttta agtattcact taacttattc
     7981 tcttctatcg ccagtatttc tggaactata taagaagtag ccacacctaa atcagtagat
     8041 aacatttctg tcttagtggc tcttccttga ttatcattaa ttctagtatc ttcatgttgt
     8101 ttcatatgag tatatacata taaattttga acaatcctag atgcattttc agagatattt
     8161 aatactttat ataagttctc cttactctca gaaagcttcc ctttatattc ttttacttct
     8221 tggataagat tttttacttt acttatgtct ttttcaattt cctctttact ggagtacatc
     8281 ttatctatct tccatttgta cttttcttct attgtacttc tatcagttcc catatcatac
     8341 acctctattt taatttgttt tatattttat tcactttaca aaatatattg tatatatttt
     8401 agtccaaata atctacattt attactcctt ctatcatatt tatttctttc ataacgtcgt
     8461 gtaagtgtat tctcttagca taagatacaa aaaaattaac cttccagtat tcttcattac
     8521 gacattctat ttccatattt cttacgataa gttgattttt ttcaaattta tcatataaaa
     8581 aaggtataga atccaaattg cttaatttta atatcaatac attcttttct tggctaaata
     8641 aaaacgtatt gattatccta agcacaaata atgctatcat tacaaataat ccacctagaa
     8701 ttgccaattc ataataacca tatccaactg ctataccaat acatgcagtt gcccacatcc
     8761 cagcagctgt agtaagccct tttactccac ctttattttt taaaattgct cctgcaccta
     8821 aaaaaccaat tccagataat acttgagcag gcattcttgc tgggtcagca tttgtataca
     8881 aattataatc aaagaataat tgaatcgaag ttattgaaac aatacaagaa cctattgcaa
     8941 ttagtatatg tgtcctaaat cctgcaaact gtcttatttt ttctctctct aagcctatta
     9001 ttcctcctat tattagtgct aatagtaacc ttattgctat ctccttactt cctatatcaa
     9061 tcataatata aaaccctcct gacttaatta caacttatgt cctacagata atttacacat
     9121 cagaaatact attttatatc ttaatattat tattaatata tgtaaaatat agccatctgt
     9181 taatttttga tacaaaaaaa cccacaactg tgggctattt gcttaggtac atgtatttat
     9241 ttattaatat atctattttc ttacttgatt ctactaactc ttcttgaaat atttctggat
     9301 attctatgta tgtatttatc tcttccctaa ctgcttcaat ctcatttttt aacgcttcta
     9361 attcattgtt cgccataata atcaccacta ttacaaattt gtatattttt atatacattt
     9421 tatcaaacaa tattatattt tgtcaatatt atgtcaataa aacttactat aaaaatgctt
     9481 taatttaggt attgtatacc tggatactaa gcctacaaac ataccagtta ctaatgatgc
     9541 cattaacata aatggtaagt accctatagc aagtatattt tctatgatta aacttgcaac
     9601 tattaattga cctatattat gccctattgc acctataatg cttatcccta ttagtgataa
     9661 atcttttatt ttactcgcta aatacataaa taaaagactc agataagctc ctcctatact
     9721 aaatagtaga tatgataaag ggcctgcaaa tattgatgac aaaattaccc tgatagtaac
     9781 aattataaca gtatctttga cacctaacaa cattaatgaa attaaagtta taatattgct
     9841 caaacccaat tttgcccctg gaaatataac tataagagga tttggtatat atgtctctaa
     9901 tacatataaa gctaaactat accctatcat aagacctaaa aatgtcattt ttctagtttt
     9961 cattaagtta actcctccaa attttatcta tcatcttaca ttataataca aaatatataa
    10021 aaacacctaa tttagactaa aatattttta atctatttag aataaatatt agttatttca
    10081 atgtgcaatt acctctataa atcttgactt tttgtatcat acttgttaca attttaatga
    10141 taatgaattt cattaggagg tgttaataat gactgtttac gatttaaaaa taggagaaaa
    10201 aggatttatt aatagtatag atggagatat taaattatct aaaagattgc ttgcacttgg
    10261 atgtataaat agtactgaaa tagaagttaa aaaggttgcc ccacttggag acccaattat
    10321 aattagattt agaggctttg accttgcgat tagaaaatct gatgctaaaa atatttcttt
    10381 acaataatag aggaggagct acccgtgatt aatgtagcat tattaggaaa cccaaatgtc
    10441 ggtaaaacta ctgtttttaa tctacttaca ggttcaaatc aatatgtagg taactggcct
    10501 ggtgttacta tagaaaaaaa agaaggattt cttggaaaag agataaaggt tgtcgattta
    10561 cctggtatat atgcaatgga tactttctca aatgaagaaa aggtttctaa atcatattta
    10621 gaaaatgaag atgttgatgt aatagtaaat gtagttgatg cttctaattt atcaagaaac
    10681 ctttacttaa caactcaact tatgcaattt aacaagccta tagttatatt gcttaatatg
    10741 ttagacattg cagaatctaa aggagttaat atagatgcca aaaaattaag tgaagaactt
    10801 ggagtaattg ttgttccaat tatagcaaaa aagaaaaatg gtgttgatag gatagaagaa
    10861 gagattaagg gagctacgca aaaagctttt atatatcaga aaaactttgg aagtgaaaca
    10921 gaaacatata aaaaattaga atccatttta tcgagatgta caaaaactac atatacacaa
    10981 aagaaatcta tcagtgataa gatagacaat atagtcttga atcctatatt agcatatccg
    11041 atatttattg gtgtattgtt cctcttattt aaatttacct ttgactgggt cggaggacca
    11101 ctacaagagg gatttgctag tcttatagag gcttatattt ctgcacctgt tagtgatatg
    11161 ttatctaact ctagcccatg gtttaaatca cttatagttg atggaataat tggaggtgtt
    11221 ggtggtactt taccattctt ccctttaata tttacattat tctttggtat atcgttgttt
    11281 gaagatagtg gatatatgtc aagaacagca tttttaatgg ataaagttat gagaaaggtt
    11341 ggtctatctg gtaaagcttt tatacctata gttatgggta tgggatgttc ttctccagct
    11401 ataatggcaa ctagaacatt ggaaagcgaa aaagatagaa gagttacagc tcttattgca
    11461 ccacttatga cttgtggagc aaagctacct atatatgcac tatttgtagc tatattcttt
    11521 ccacataatg ctgctttagt aactacatca ctgtatttgg tgggtatagt tatggcaatt
    11581 gtagttgcat tattcttaaa taaaacagcc tttaaaacag aggctgaacc atttatatta
    11641 gaattaccag aatatagaat ccctactata gatgcactta ttaaaaatac ttggaataaa
    11701 tcaaaaggtt tcttgataag agttgttact gtcatgtttg caatgtcagt cgtaatttgg
    11761 ggactttctt cattcaatgt ttttggatat acagaagata taaatgtaag cttcttggct
    11821 actctaggtc atattatatc tcctatattc aaacctcttg gttttgatga ttggagaact
    11881 tcagttgcta ttttaagtgg tcttggagca aaagaaatcg tagtaaactc tttaaatata
    11941 ctatatggta atctaactgt tgtcttacca acaatattta atggagttac tgcatataca
    12001 ttcttgatat ttactgcttt atatactcca tgtatagctg cacttgcaac tatgaaaaaa
    12061 gaatatggca ataaaatgat gtttacatca tttgcatatc aatttatact agcatggata
    12121 atggctttta tagttaaaag tataggtgga gtactattta tgggtaattc aataatagaa
    12181 gctttgatag gtggaataat tatagtcctt gcactcttta ttctgtttaa taaattcaag
    12241 tctacaaaaa acggaaatgt ctgtagtgga tgttctattt gttctggatg tccaagtgct
    12301 aattcttgct caagtaagga agacagcaat aaaaaaaata atgcacacta atatataaat
    12361 attttagttt aaaataagat aaaagtgggt gtctcaaaat taacttttta gttcatgagg
    12421 cactcttttt tatagaaaat caaatatatt ttcatcattt caacagtaaa aaagagctta
    12481 tctagtaaaa aagaggctta tctctatttt gagacaacct ctttttttgt tacttattca
    12541 gtttcttgct acctaacttc atgtggaacc tagtatcttt tggaacactt gggtctacac
    12601 ctcttaatgt aggtatttcc ccacatatta gttgtatagg tactataaaa ttaaatgttt
    12661 gatagtaagg attatctgtg atatcaatct ttaaattttt atcattttct gtaacatctc
    12721 tacctatagc aaatacattt tccgtccaac cagaaagtac atcaatcatc ttagttactc
    12781 taggttcttt tcctgtatct agtataaata tagtagaatc actatttata gcattatata
    12841 taccatgtat aaactcttca aactcatacc cagttacagg tattctcatt gtttccaata
    12901 attttaatgc tgcttctaat gtatctccat atatatcaga gtgacctatt attcttattt
    12961 ctttagaatt aactaacttt tctttgtttc tttcaatcca ttgtttagag agcttataca
    13021 ctgcttcaaa tctattaatt gcgtctaata tcttatttat ttcctcattg tacttttcac
    13081 tagatattat acctttttct ctagctattt gaagacctaa taacattaag tttaactttg
    13141 tacaataata tccttttgtt ttagctccag atttttcttc accacaattt actgttaata
    13201 tataatcaga aatctcatct attaaagcat tcttacaacc tgccatagat gctattttac
    13261 agcccttatc ttcagctaac ttcatagcgt tgtatgtaga ataactactc ccaccttgag
    13321 atacacctac tactagggta ttttcattat caaacttgaa tgtatcttca gttatcataa
    13381 atggatacat tttcacaaca tctatatcta ataaattttg taaatatggt tgtacttgca
    13441 ctcctgagtg atagctagta cctgaacctg ttattataat cttttttaga tttgttctct
    13501 taacttcatt aaataagcta tctgcattag atataatttc tctcattcta actggtgttt
    13561 ctaacatata atcttgtatt gtcattttat acaccttcct tatctatata ttcaataaat
    13621 tataattaat ttctataaca aacctatagc atgtaaagcg attcctgtta ctatagtgcc
    13681 tatagttaat tttgttacac ttacctttct ctttaacatg tagtatatta caaatgcaaa
    13741 ggttaaaggt aacatattag gtattatttt atctagtata tcttgtaaaa ttacttctgc
    13801 accatttaag ttgaatttca gaggagtagt tatactcaac atagttgcaa ccattcctcc
    13861 tacaacgaat aaaccaacag ttgtagccat agacattatt ttctccataa gtccctctct
    13921 ttgtattctt tcaagagaat ttacccctac ttgataacca tacttaagtc caaatattct
    13981 aagggcaaat gctggtatat tgtataatag taagaataat agtggtccaa agatattacc
    14041 ttcttttgca agtgatactc ctactcctgc tgctataatc ctaaatgttc cccagaataa
    14101 tgaatcacca attcctgcaa gaggtcccat aagagctgtt ttaacagcac taattgaact
    14161 ttcatcaaat tctggattat tagcattctc ttcttccata gctgctgtta tacctaatat
    14221 agcaggaact actactgtcg ttgtattaaa aatctccaaa tgcctttcta ttgcttttgc
    14281 ttgattttct ttcttattgt atagcttctt tattgcaggt aacattgaat aacagtaacc
    14341 taagttttgc attctttcgt aattaaatgc accttgaaga gcaaatgaac gccaaaacat
    14401 acttcttaat tctttttttg ttaatttatt caaagtcaat ctctccttcc gttaaagtat
    14461 tgattgctaa ttgaggattt tgattatttt ctttgttatt gatataaaag tttagtacaa
    14521 atgctacaca tactccaaat aaagctatag cagttatatc taatccacca taagatgcta
    14581 acaaaaaccc tataaagaaa aatacagaca tcttttttga aagtaacata tccataagca
    14641 atgcaaaacc tactgctggc aacaatgttc ctgcaacacc cagtccgtct aatataactt
    14701 taggtattgc atttatcaat gctgtaactt tatcagcacc aatcataatt gctaaaaagg
    14761 ttggaactga tgtacttaaa aagaataata ttactggcaa ccacatcatt atggtaactt
    14821 tcttaaaatc agcttgttta gcatagaaaa ctgccttttg agaaaagtac gaatttatta
    14881 ttctaactaa tactccaaga gattgtgcta agactgctac tggtactgct actgctaaag
    14941 caacttctgc accattacct gataatatag caaaagctgt acctaataca cctcctgtta
    15001 ctacatctgg aggagtagct gcacctattc ctgcaattcc catccaaatt aattcaagct
    15061 gtgcaccaat cataattcct gattttatgt ctcctaaaac taatcctact aacatacctg
    15121 taactattgg cctatcaagc atagattgac caaatatacg cccatctagt ataccaactc
    15181 ctgctataat agctaacata gtagcttgta ctaacatatt gaacctccat tttaacttag
    15241 tttaatttac attaaattac attacatttt tgattttgtt ttgattttct aataactcat
    15301 ttacataaac aattccattt tttgcttcct ctaatcctga tttaactatt agttttgtat
    15361 cctcctcaac taataaaagt atagataaaa gcattggtaa atttactcct gtcaataatt
    15421 caaatttgaa cccttgtaaa agtaagtttg tacatatgtt tgccacactt ccaccaaaaa
    15481 catctgttaa gacaacaaat tcttcactat ctttattatt tgataatata cttctaattt
    15541 ctttctcagc atcatctgct gatttatccg ttgtcataca aaaatactca atattagtta
    15601 atgaaccaat tattaattta cttgtgtcaa caagctctct ggacaattct ccatgtgttg
    15661 caattagaaa ttttttcatt ctctttctcc taaattattt tattaaatct gatagttgtt
    15721 tctttttgtc tctaggtaac attctaaatt ctatatcaat ccctaaatca cctatttgtt
    15781 taaagttatt tatatcatca tcatcaacag caactaaatc tgtaagtttc ttcttccctt
    15841 cctcatatct taatcctcct acatttattt cttttaatac tcctcctgaa tttttagcta
    15901 caactaaagc atctttggta ttttttacta acactaatac attgcttttc tttgaagaag
    15961 caaatttttc tactatttct ccagattcct ctacccccct tatatataaa gttacattgc
    16021 taggtttcgc taagttcagt gacatagctt tcatttggtc attttttgct tcatcatttg
    16081 caactagtat tacatttata cctagtgatg ttgcccatcc atatgcaacc tgaccatgta
    16141 tcaatctgtc atcaattctc atcaaagata ccattttccc caaatcctcc atcatacatt
    16201 aaaattttta ttgtttatac tctcatttat ctataaaagc aaaaagtatg ccaaatactg
    16261 gcatactttt ttaaatttca ttcacttatt ttgtaatctt gtattttact gtggtagacc
    16321 atagataata ttataaatat atcctatctc tgaaattggt atttgcacac tataactctt
    16381 ttctagcaca ctaaatgcat tttttattag ttttacttca ctatgatggt ttttgattaa
    16441 caaatccata tcagtatact cttcaatttc agtttgtcta actaatcttt caatcataca
    16501 acttaagtga acatataaag aaatcttttt atcatttgaa agttttttct tttgaatatt
    16561 ttctatagca ttaattcctt cttctattct ctctattatc ttttttgaat ctaaaatagt
    16621 tagactatct attactctaa ttaatgaaaa attctttaca atagaatcat ttatgttctt
    16681 tattttttca ttttctacta ctctgctaaa tattctaaaa acatcatctt ctccttttcc
    16741 tgatattata tcctctaata atataaaatc taccccattc acatttggat tggcagttcc
    16801 tactatagcc atcacatcat acatttcaaa aactggttct ttatctccca tagaaacaag
    16861 actatcataa tcataaggta ttattttgac atcaactatt ccaattaaac tttcggaaag
    16921 tagatgttgg atttttatag cagtacctat ccctgtaaaa caagatgata ttatagcata
    16981 ttctttattt ttttgaggat aatacaattg tatattaggt ttagtttctt catccataat
    17041 ttctgcaatc tcttcaatgt ttttaccttg cattaataaa tttcctactt ctagtgcaga
    17101 aatagtggat atttggtcta taaataacat tgggctattt atcttattct ttaaatcttc
    17161 atatataaga ttaagagagc ccatatcaaa taacacaatt aaaccattct ttatcctatc
    17221 tccctctagg tatctagaca tttgttttga tacatccgca atagtagcat ctataggcat
    17281 atcaatagct gtaaatatat ttttacccaa catccgatta catacattcg ccatactact
    17341 ggcagtagca tatccatgtg ctataatagt cgcattaact tgattaaatt tcattcttat
    17401 aattgttgat ttaataaata taatcattat gattatatct acattattaa gatacatatc
    17461 caatctattc gttattaatg ttgatatttt acttattata gagtattctt tgtaactctc
    17521 tttttttaaa tatattagta atttattttc taaagcttta tactcatcag acaattgata
    17581 agattcgtcc ttcaaataaa gataagacga aaatgccaat acattattcc catcatattt
    17641 tatattgtta ttaacttcta aatttctaaa tatatcctgc attatcgata ttataagctc
    17701 cataagtacg ttattatttt catcattatt tgaaaagact aaattgtcca ttagtctaat
    17761 cattagatta gaactctttt tactaaattt ttcctttgtt atttcatttt tttctagtct
    17821 atcaaagtaa tctaaacatt ttatgaaact ttgatagatt atatttcttt tatttcttcc
    17881 atttgacatt tcaatactaa attgtttagg agaaaagata atgcttttgc ttttaccact
    17941 ttggaattga aatatatcta cattttctat ataaagattt ctaggaaggt cttgaagagt
    18001 aacaaaaata tttccatttc ttctttgatt ttttgcataa gctcgtccac atgcatattt
    18061 tattacattt ttacattcac ctatattccc cttgaatttt atctttaaaa tcgcatttaa
    18121 tgtaatttga gttatctcta taggcaaacc aagtacctta ctttcttcat aaataaaatg
    18181 ttctacaatc tttctttttt caatagtccc tctttcttct atattgggaa cataaatatt
    18241 tatgggtatt cttcttaaaa aggtttgtaa aaattcagat agactttctg ttgtagcaaa
    18301 tattaatctt accctagctt ttctaattac tccattctct ccaatcctag aaaacgtacc
    18361 tgtatccata aatgtaaata acttttcctg accttcacta tttaatctat gtacttcatc
    18421 taaaaataaa acaccaccat ctgattgttc tattagacct gctctagatt catttgctcc
    18481 agtatatgct ccttttacat gcccaaataa tagactagac aataactcca aattattata
    18541 atactgcgca cagtttaaca cattgtatgg agaatttttt tctaatacat tactgcttac
    18601 actatatttg tatattatct tggctaaaaa agtttttcct actccacttt cacctgtcag
    18661 cattattgta agtccgccat ttggataaag tacagcggtt tttatttgtt cgattacttc
    18721 ttttaagcta ttatcatatc ctatgatact attaaacaca gtctcttgct ttaataattt
    18781 acactcattt ttaagttcat ctaaattttc ataaatactt ttttttgtag gtcctactac
    18841 atcatttatt ttatttttag gtaaaaaaat aacaggtcta ctgtttatct ttataagatt
    18901 atcagatttt actaaatcat ttaataatct acttgattga gttctactta aattaaactt
    18961 ttttactata tcttctgtta ttatgccact ttctttttta agttcatcaa agttgttaaa
    19021 taatacacat tcatttacaa aacttaaaat ttcatccctc atgttcaacc tccaatacaa
    19081 atatcaataa tactataata ttagtatatc atttatatat ttatgaaaaa gataattata
    19141 ttcctatctt gattctcttt tgtaagtttg taaaatcaaa ctttttataa gtataaaatc
    19201 tagatttttt aacataatct caaggttctt atctttccat atatctgaca tagaatttat
    19261 ataagaatat atttattgta tttattataa taaaaagtaa aacattatag gtattttaaa
    19321 acttattagc gaagaaaata tttgtaactt ttattacctt atgaatatta tttcaaattt
    19381 aaaaaggcta taaataaatt tatttttaaa taaatctact tataacctca caataatact
    19441 t
//
LOCUS       DAELTA010000072        18928 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.072, whole genome shotgun sequence.
ACCESSION   DAELTA010000072 DAELTA010000000
VERSION     DAELTA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.072"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(34..570)
                     /locus_tag="KPG13_002091"
     CDS             complement(34..570)
                     /locus_tag="KPG13_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG0985807.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(588..1901)
                     /locus_tag="KPG13_002092"
     CDS             complement(588..1901)
                     /locus_tag="KPG13_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG0985808.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2256..2696
                     /locus_tag="KPG13_002093"
     CDS             2256..2696
                     /locus_tag="KPG13_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG0985809.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2880..3068)
                     /locus_tag="KPG13_002094"
     CDS             complement(2880..3068)
                     /locus_tag="KPG13_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0985810.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4090..4485
                     /locus_tag="KPG13_002095"
     CDS             4090..4485
                     /locus_tag="KPG13_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985811.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4528..6627
                     /locus_tag="KPG13_002096"
     CDS             4528..6627
                     /locus_tag="KPG13_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG0985812.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6852..12062)
                     /locus_tag="KPG13_002097"
     CDS             complement(6852..12062)
                     /locus_tag="KPG13_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG0985813.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12077..14572)
                     /locus_tag="KPG13_002098"
     CDS             complement(12077..14572)
                     /locus_tag="KPG13_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0985814.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15233..15520)
                     /locus_tag="KPG13_002099"
     CDS             complement(15233..15520)
                     /locus_tag="KPG13_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985815.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15540..15815)
                     /locus_tag="KPG13_002100"
     CDS             complement(15540..15815)
                     /locus_tag="KPG13_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985816.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16450..16629)
                     /locus_tag="KPG13_002101"
     CDS             complement(16450..16629)
                     /locus_tag="KPG13_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985817.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(16914..18389)
                     /locus_tag="KPG13_002102"
     CDS             complement(16914..18389)
                     /locus_tag="KPG13_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0985818.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
BASE COUNT         6289 a         3316 c         2074 g         7249 t
ORIGIN      
        1 ttttaataaa tctttttctt ttaataaatc tatttaattt tcttcttcta tatcagagcc
       61 aaagggaatc catgcattac caaactcttt atctttaaat aaataagcta atccagttat
      121 ttgcttcaat ctaagaattt catcaacttc catacccagg tgctttgata tccattcatc
      181 agattttcca atttcacaaa gttcagttat aattttactc attaaatcca cattatgaga
      241 acctcttgca cgattgtgtc gaatagtact tgccattctt tcatcaagag atttatcaat
      301 tactgaaaca ggaagaagtc cattttctct atcatatata tcctcatttt caagcattac
      361 tctatatcta tgaaaaccat ctacaataat atacttatcc ttttcctcat tataataaca
      421 gacaattggc attgtatacc catcttcttt tatactttta tacaacaact gcatttctgg
      481 tggggctact gcatttggat tatagtcgtt agattctatc ttttcgattg gcactgcgat
      541 gattccatat acaggacttt tatattccat agcatcttaa ctcctatcta atgaattttt
      601 tcatatttct tcttaatata ttccgttcta attctctgtt cttttgtagg accaaatccc
      661 ataaatctac acatatggtc attttttaaa atacaataac acattctttt ccaacttggt
      721 atatctttgg taaatttaat atcatccgta tcatcaggta tatcatccaa aaatatgact
      781 ctactctttt tatcttttgt atgatttgaa actccattta attctatttt ataacctttt
      841 ctcaacaatt cacgtataac tatttctgac aaaccaccac ctgtattctg ccaaaactca
      901 atagatgttg aaaatttttt aatatagcta ttccttatat tttcaggtaa ggtatcaagc
      961 aaaaaatatg tgaaactttt ccatgtgtgt ccatatggca atttaacact acgatatccc
     1021 atagcttttg ttttgccata aatagaagta aaattaactc cttgtactct agcaaccaat
     1081 ttcacccata catctgggtc caatattctg tataaattta aactctcttt tgctgaatca
     1141 ttaaaaggtg atgccactct catttttctt ggagctactc cagctttata gaataagtca
     1201 taaattttat tatagtcata gtcaaattta tagtgtgcta accatatatc ttctatttcc
     1261 caatcatata taggagatgc cgtccagaca tttttaaatt ccttagttat ccagcacttt
     1321 cctttatatc catgctgttt attcacaata gaacaataac gttgtagaga ttctgctgct
     1381 cgtgttccta ataagcaaac aacttttgca ccttcatgta tatctctaat ccatctacca
     1441 aattgttttg ccaaactttc ttgtctcatt ttatatttat aataagtaaa aggattattt
     1501 ttttgattta ttatgtattc tttgactggc atttccctta cccataactc ttctttctca
     1561 tcatcccatg gataccaata catttcataa tttcctacag cagttcttgt agccattggt
     1621 aaacacaccc aatatggttc aatatcttca atattatctt caaatacttt ttcaacatat
     1681 tcagtagtca aactatattg agcctcaaaa tcttgatgaa atactcctat tttggaactt
     1741 atattattct ttctcttata atccaaaact aaatttagca gtaaaccact atcttttcct
     1801 ccgctaaaag atacatagat ataatcaaat tcattaaaaa gataagaaag tctttcttga
     1861 aaagcttcaa atacatcgga ttctaaatat ctcttatcca tttgaattat cccctttcaa
     1921 ttagaagttt ataaaacctt acaatgcaat attattatgc ttttttagca gtaatcagca
     1981 taaattatac attatttaaa tacacaaatc caactaacta catataatta taatgtaatt
     2041 atacttttgt attataaact atattttatc attattttac atttttttcc aatatatata
     2101 aatatcatat ttttctatta gctaaaacta attactttat atttctacgt attagtataa
     2161 taatacataa aaacttgaat aatgcataaa aacttgacat actgttgtca taattacttt
     2221 gctataatta aaagtaaaaa tggaggtaaa ttctaatgta tgaacgtatg cttgataaaa
     2281 gcaataaacc ctgtttgtcc caaataatag aatatatagg taaagatgga tatgcacgat
     2341 tgtgtaagtt ggaaacttac ttgaaaactc aatataattt atcaagagaa cttcgatttc
     2401 cttttggaaa caattatggt tggggttaca aatatagcca taaatctttt catttatttt
     2461 atgctttttt tgaaaaaggt gcatttactg ttctgattca gataggtgac aagcaagttc
     2521 cagcagtaga aaaaacgttg gtagctttga caccaaaagc taaagaactc tgggtcactc
     2581 gttatccttg tggcaataat ggaggctgga ttaagtatcg tattttagaa gatgcagact
     2641 tacttgatat tattcaatat ataagagcaa aaaaatctcc acctaaacaa aattaacttt
     2701 tattttttgt gatttaaata tagtgtatat aaattccatt ctttccatat taaaaaataa
     2761 tataaaaaat atctattagt ataacttatt tagtatgtct tattctactt tttacagata
     2821 attaaaaagt tttatcataa ataaatcata actagacatc ctagtaaatc aaaattaatt
     2881 tattcttttt tattcgaatt tttgataata gtccctatac atatgccaat tatcattcca
     2941 aaaattagac cataacacat tgtaatacct acatttcctt tagaaatacc tatagcacaa
     3001 cctatagcca ttccaagtat catgcctata aacatacata taataactaa attgttgttt
     3061 ttattcatag tttttctcca ttcaatatat tttaaattgc ctagctcgcc ttcttagctt
     3121 aaaacaaaga aatataaatt aactttcttt cattctaata tctgcctgta taaatgtcaa
     3181 acaacacatt tcttgtcata acctcctctt tttgaagtaa gtacaatctc ccagacgcaa
     3241 taatatcaat ttcctaaaac catttaagta acttttatat ttacataata tttacaatca
     3301 taaactatat tgttattcat ttatacatca taaacactcc ctcctaatca ttattatact
     3361 taaaataatg ttcatttata ataatatatc taacgcactt taattgaaaa aactaaaact
     3421 ttgcctttca ttcccatata taaataagta tactttttga tgctctatta caaatcaact
     3481 aaattaattg atataaatta tcattttctg ttgaaaatac cattcgcaca tgctatactt
     3541 aattgcataa atggtaataa atgtcatatg attttacaaa tctattaata acaattatta
     3601 cttttactgt attttaaagt ttttggacta aatatcaaga actttctaaa atacaaagta
     3661 caaaaaattt atataagtta taaatttata tgaactagat aagctcaaac aatatgttat
     3721 gtataaactg tgtagtattc ggttataaat ttatataaat tatataagct agataaagta
     3781 ttacctcaac tatataagct agatatataa attgcttata taagctagat aagaattatt
     3841 tcaactatat aaactacata tataaattac atacataagc tagatatata aactgcatat
     3901 ataagctata taagaattat ttcaaccata taaactacac atgtaaaatt catataaatt
     3961 tactaatata aatcaaataa aattactaaa ctctctaaac taattttgta aaacatactc
     4021 tatttttaac atttgttacc ttatacataa gtacaaacaa aatctaacta aatgaaagga
     4081 taatgtaaaa tgaatatttt aaaaatgaaa aaaacaggga tatttttagg aggagtactt
     4141 tttggtactg taggtgttaa gatactttca agtgattgtg ttaaaaaaac ttgcactcgt
     4201 gcaacagcag gagcattgaa agttaaagat tctgttcttg aaacaactac gaaaattcaa
     4261 gaaaatgtag aagatatttt agctgaagca aaagaaatca acgagcaaga agacttatct
     4321 acatctaagg taaacgttgt acaagatact cttaaatgcg aacaagaagt tcttaaaact
     4381 gaaactataa aaactgtaga agatactaat tctaatagca ttaaatgtga agaaatcaaa
     4441 gataacgtaa ttgaagaaaa aaatagtact tgtacaacag aataaattta atgattatta
     4501 tgattaataa tatcaggagg aagtaaattg aatttttata taaaacacga aataagaggt
     4561 cgtatacgct ttgacttaca acttggcaaa ttaaacaaca agcagtccga tactctctta
     4621 tattatttaa cttctataaa tggtattacc aaagcaaaag tttacgatag aactggtgat
     4681 gcagtcattt attacactgg tgaaagagaa tacataataa gagaaatcac taaattcagt
     4741 tttaacaata gcaaaataga aaacctagtt cctgaacata ccagcaggga attaaacaat
     4801 tattatagag acaaattagc aaataaactg ctagtcaaaa gtatctccaa aatatttttt
     4861 ccaaagtcct taagattttt tttaattgga cttcattcta taaaatattt taaaaaaggt
     4921 ctctcttccc tgctaaataa aaaaatagaa gtttctgttt tagatgccac tgctataggt
     4981 atttctattt ttagaaaaga tatcaatacg gctggttcag ttatgtttct tcttggtatc
     5041 ggtgaactat tagaagaatg gactagaaaa aaatctatag acgacttggc tcaaagtatg
     5101 tcactaaaca ttgaaaaagt atggttaaga aaaggtgaca ctgaaatctt aattccaatt
     5161 tcagagataa aggaaggaga tttagtaagt gtaactatgg gaaatatgat tccattggat
     5221 ggagttatag tatcaggaga aacaatggtc aatcaggcat cacttacagg tgaatccctt
     5281 gccgttaaca aaaaagaagg ctcttacata tatgctggaa ctgtaataga gcaaggcaat
     5341 attgttatgt gcgtcaagga aaaagctggt actactagat tccagaaaat agtaactatg
     5401 attgaggaat ctgaaaaact aaaatcttct gtagagagta aatttgaaca tctagcagat
     5461 acattagttc catacagctt tttaggaagt atattaacct atgcaattac tagaaaccct
     5521 ataaaatcac tctctatatt aatggtagat ttctcttgtg cattaaaact ctctatccct
     5581 atttcagtac tatcagccat gagagaatgt aacaataaca acattactgt aaaaggtgga
     5641 aaattccttg aaggtgttgc atgtgcagat actattgtat ttgataagac aggcacatta
     5701 acaaaagctc aaccaactgt ttcagatata attacattcc aaaattataa taaggaggat
     5761 atgttaagac ttgcagcttg cttagaagaa cactttccac actccatagc aaatgcagta
     5821 gtttacgaag ctgaaaaaca agggcttagt cacaaagaaa tgcatacaga ggtagaatat
     5881 attattgctc atggcatttc aacaaaagtt gatgataaaa aggttgtaat aggtagcaaa
     5941 cattttgtat ttgaagatga gaagtgtact atcccagacg gtgaggagga taagtacaac
     6001 aatctatcag atgagtattc acacctattt atggcaatat caggtaaatt atcagcagtt
     6061 atttgcataa atgacccttt acgaaaagaa gcaaaatatg taatttcaaa tttaagagaa
     6121 tgcggaataa aaaagattgt gatgatgact ggagatagcg aaaaaacagc taaatctata
     6181 gcaagtaaag ttggtgttga tgaatattat tcagaagtct taccagaaga caaagccaat
     6241 tttgttaaaa gtgaaaaact caaaggtcgc aaagtaatta tgattggtga tggaatcaat
     6301 gattctcctg ctatttcaga atcagatgtc ggaattgcaa tgagtgaagg tgctgaaata
     6361 gcaagagaga tttcagatat taccatatca gcagataatt tgaataattt aatcgtttta
     6421 aagcagatta gtaataagct tatgaaaaga gttgatttaa gttctaagtt tattattggc
     6481 tttaatttag gtctgatttt acttggtgta ggtggatttg ttagaccaag tacatctgcc
     6541 tttttacaca acacatctac tgtcggaata agtttaaaca gtatgacaaa tttattggaa
     6601 aacaccaata cttataacta ccattaatct ccattacata aaacatatga tataaaaaat
     6661 ttataattca tctttttaaa agaatatgtc ttaaaattag cttttttcta atcaaaatta
     6721 tataaagcat actttacaaa ttttaaactc aacttatttc tattttttac aaaaaaggag
     6781 atgattcaaa ttttcatttt gaaacatccc cttttgtaaa tttctttaaa atttatatat
     6841 taattatatt ttcaaccaat aattgtattt tcaaccaatc tttcttcata gcattttaaa
     6901 tcattttgct ttagataatt aaattcagac tctacataaa taccacagcc taccaattca
     6961 attgtcaaat taggtatatt ttcctttagt atactagtct gctcataaat aattctttcc
     7021 aataaatcaa cttgtaatat agaaaaatgc ttcataatct ccccatcact atctatatct
     7081 ttcttgattt cataccaact ctttgacaaa attcccttca aatcaatata ccctagactc
     7141 ctcaatatat ctatactatc gctccatgca tacttactag gtttatcaaa gtatgattct
     7201 agtttcttct tctgctcatt tgtaagtgaa tcataagaaa gcttcttatt aaaatgtgcc
     7261 ttcaaagaaa tcttagccat agaatatgga cttctaccag agtttaatcc tgcatttttg
     7321 aacttttcaa ttcctttatc ttctactgaa gaccatattg agtttattga atctgaatca
     7381 ttgtatttaa tatcaagacc atgtgtaata tttatatagt tttgtggaaa tacatattga
     7441 taatagacat cttttttatc atcatataga gagaagtatc cactatcata taaatgaata
     7501 tgcaccttta cttttaaact atcctctaaa atatttcgtc taaggttcgg gatatttgca
     7561 tacatggtta catattttat ggcatcaaag tttttattta atggaacttc aaaactaatt
     7621 tcatctcctg tcaaaagctt tttcacttct tcttcattac aatcatatag agaatatgta
     7681 ttttcaaatc ccacacgtga tttcccatct aaactgcctg ttaatgctac cttcaaaata
     7741 tggttatctg ttttttgcca ttggtaagca tcttggtcaa gaaactctct aatctgtcta
     7801 ttttgctctt tcgcctgatt tataacctcg ttttcatctt ctgtaatccc tacatggcat
     7861 ctatttaaga tatttactat attgcttcca aatgggaatg tccatagttg aaaagctgct
     7921 gcctcaacct gacacaaata tctaacctga tttaattcaa tttgatggat aagttttttc
     7981 tgatattcat tcatctgatt accactatct gacatttgat aaatcaattc tcccattgca
     8041 agattatctt ttttcttatc aatcttatca gtaatgataa tttgagtttc catcatatcc
     8101 tgtacttttt taaatattat agaaatctcc tctgagtttt ccatttttaa gcttccaaac
     8161 ttcgtgctta attcagttaa aacttccttc atctttaatt tacttatctc attttcaaaa
     8221 atatcttgat ttttaactgt actatcgaat ttttctacaa atggtataac agattctttc
     8281 aaagttttat ctttaaattc caccatttga gtattcaatt catctaattt cttaatatta
     8341 ttttctttat ctttaattgc ctctttaact acctctattt tactctcaaa actagatttt
     8401 acattaagac aattattaac accactaatc gcagtattta taagagaaca agcagaactt
     8461 ccaatactca atgcattttt aattttagat gtcttttgct gtctttcaat agcttgttta
     8521 cttttctcta gtgcagctaa cttatctttt tgctgtttat tattgtaatt tgcctccaac
     8581 atacctattt cttttttctg gatttttgtg taaatttcct tatactcgct ttcacttaac
     8641 ccctcttttg ctctttgaat atcgtcagct ttgtctgcat agttatcatc atctatagac
     8701 ttaactataa tctccttaat ttggtcaaat ttagttcttc tttcttcatc aacacctgcc
     8761 aaattataag tcattttatc taaagcattt tttctatctt cacttagctg ttctccaagt
     8821 acaaatctca taccatcaga atccaattta aaatctgaaa aatcaaccat cctgttagtc
     8881 cattggtatt cctcttcttt agctgaaaga ttttccttca aagattttgc actgtcaaat
     8941 gcccctagac aagcttgacc aacaccacta aatgcagtca gtaaactcaa tgcgctttgt
     9001 attccagaaa gaattaactg tgttttcatt tgttcctcaa gttttgcctg tttctttttt
     9061 agttcctcta tgtcttcttg ttcatcttct ttcatcttta taatttcttg aatcaacagg
     9121 ttcatttttg cattaacaga aagcatttta ttctcaagtt cattttttaa aacctctatt
     9181 tgttgatttc cagattcaat ttgagttagg atactttcct tatatgtatt gaaatatcct
     9241 tcttttaact catctttctt tccttgttcc aattctctta aggtcttatc cagatagtct
     9301 gtcaaagaac gaatatctgt aacacgattt cctccaattt cactttcttc aatcatctgc
     9361 acataaaatg ctgtcttctg ctcaagtgca aataaaatca tactccattc gctaaaattc
     9421 tgattttttc ctacaaaaga ttctattcta tcagactgca tcaatttatt tataaggtat
     9481 tcatagattt cctttgcctc tttataaaat tctttcttta tattcatgtc atccatagaa
     9541 agtactgctt tatttttctt ctcaaaacta tagtacttct gaagtatttc tctatttaat
     9601 tgcatctcta ttttctcaac tgcataaaaa atttcataaa tatgtgaaaa ttctaaaata
     9661 tcagcttcta aaattcctac tgcacacaac agtctaagat ttctataacc tttaattcca
     9721 gaaaaaccaa atctctcgaa aaattcatag aagaatgtat ctattgacaa agaatactcc
     9781 tgctttaaca attccttttc ttttagtgca tctccagagt gaccggcttt tccctctgct
     9841 ccggctgaac ctgaatcttc acgtttgcta attgtccagt ccttatgttc ttcataaaga
     9901 gtatttaatt tctctccatc atctgctgct tgcttctgat ttaaagcatg attaaacaca
     9961 tatctatcaa aataatagta gtttctatca ctattatcca ttggaggaga ggtacctata
    10021 tatcttattg tttcttcata tatacctcta attccaccaa gacctcctgc accaccagct
    10081 cctccagagc ctccttttcc tgctaaacca tgatttccat cttttttact tatagcactt
    10141 tttaataaag gagcttttcc aaggagataa acctgtccag acttgcttcc aagacctgca
    10201 tttccaccat tacctcctgt gcctccagtt ccaccttgtt ttcctttttc tgcttttaca
    10261 acatagtatt ttgtaccttt aacattagca aattcttttg actctttttt agaacctatt
    10321 ttagcatttg ccccagcttc tccagttatc cctgtatctc ctttttttcc atcttgtcca
    10381 tttccaccac gtccaccatc tacgtaaagt ttaagttctc ctcttttttc tatttcttta
    10441 aaagataata tgatatcacc tgaagtacct gcattattgc catttcttcc attcattccg
    10501 tcgtaaatag aactttctcc aaccaatttt tctgcatcct taccagatac gttgattgaa
    10561 taagtccctt ctggtatatg taatttttct gtaagtagta caatattctt acctgagaag
    10621 acttctttct ccatattaca gtcaattttc aactcacaat ctccatacat atatatagat
    10681 gacaaaatat taagttgata accagatatt attttagtca aatccgataa attcacttta
    10741 ggtataggaa tatatataga aagtacattt tctttctcat atgattttat atatgtacaa
    10801 ctttgaatct tctctatcct atactctgca tcttcaagct ttttggcttc ttcttcccta
    10861 tattcttctt ctgctatcca atatttgata tcttttaatg tatcttctaa agtatttttt
    10921 agcaatgatt ttaacaactc acttagacca acaatatgag tctcactaca gtaactattt
    10981 aactctaaaa gaagacgtgt ttcttctaat atattcactt tctgtacgcc atcaccatat
    11041 gaaagttctt gtacaaactc cataagctct ttcatattat tagtcctact acataaatct
    11101 gtatacatct tatctaattg aatcttttta tcttttagac tatttataga agatagaatg
    11161 gaaatagaat cttccatacc ttttttaaca ctttctatat aaggaactaa aagtttttct
    11221 aggtctttat aggttacatc aacagctttt tgtatttcta ttttacaaga agctgtaagc
    11281 atcaatccca aatctgtttt ttcaaatgtt tcaggtagat ttttcatttc atccaacaac
    11341 ttttctggct gtttatctag tggattacat aatttaatag atacaccatc tgataatgct
    11401 acatttaata agtacactaa ttccttacaa ccgtcgtaat ctttctcttg actatctaag
    11461 tcttcttttg ctttttccaa tatatttctt attttttctt ctgttacatt accgattagt
    11521 gttttaggta ttttattaat aagaaagaag aaattttctt tatactttgc ataatcacca
    11581 agcatatctt tcaaattttt gcctgtttct atcaataatg tatgtctaga gtccagtagg
    11641 gattgatagt ctaagataac taccatccct ttcacctttg aagatgtgga aatagtttcc
    11701 cttacaagca tagcaccagc aatcatatac tctgctccac ggttgtcaaa aaaacctggg
    11761 caatcacata atgaaaaatc tctatttgca atatctacaa cttctggaca taaagtctta
    11821 gaatatacac catttccaat ttcgacagct gaatcttttg cagttatata tatttgtcca
    11881 gtctcttcat ccgttttttc ttccatcaca cagcctttta aataatttat acaagtactc
    11941 tttccaacgc ctgaagcacc taggaataac aaaattgact tttcttttaa gtcatatttt
    12001 ctaagctcgt ctcttccttc ttccaaaagg tttacgattt cactataaag tttatttgcc
    12061 atacatccat ccctttctat atttcggaac aatagccttg atatttttct ttaataagat
    12121 tctcttctac ctttgaaaca gctgttgata acttagtatt tggagcatta aatcctccat
    12181 tgtctcctct atctccatat tcagcgtatt ctcttgctgg tcctgtttta ccttcattcc
    12241 agcttccacc actaaattct atatgatatt taattatact atctaactta taatcccaac
    12301 ttgtaaaata tcctgtatag tatacagtct tatctatacc acttggagaa aatgaaagtg
    12361 tattatatcc gtgatagcca ccacttcctc catatccacc taaacctggt ctaccatttg
    12421 gacctgcttc tccatctttc actgttatat cacaatgatt aattattcta tttttttgtt
    12481 cctctgaatc tgaaatgaag taaatattac ctttcttacc agcatttcca cctcttccag
    12541 catttccacc agctccacct ttttcacctt ttttgccact tgtacctttt tcaaaatacc
    12601 ctttaaacat ctgtccgtgg aaataacttg tatatcctgc atcagctcca ttacttccag
    12661 aagctcctgt ttcaccattt tctccatcta ttcctttaga accatttcct ccatttgcaa
    12721 tcagtgttag acgataattt ctaatttctt ctttaatata taagtaaata ttacctgcat
    12781 ctctaccatc ttcaccattt ttcccataca tagaattatc tacaagttct ccgctctttc
    12841 cacttacatt aatttttaca ttactaccta catcaagatt gttagaagca agaataatat
    12901 ttttctctga tgcaaagttc attccaaggt tatcatccat ataaatagta tctttagcaa
    12961 agaacataaa agtagaaaca ttgtcatcta cttttctatt tttgaaatat tccaatacac
    13021 tgctaattac taagtttgga attaatgctc ttatttcaat acctttctta tcattaatat
    13081 taattgtttt tactataaat ggatttaaaa tgtatttacc aataaattct ctaattatct
    13141 cattaccttc ttctccatat gcacttaatc ttgttatact gtcttctaac tcttcatttt
    13201 gtgtaactgt aacatttgtc aaaattgaat taaaatcact tacactatat acataatcct
    13261 ttttatttgc aacttccatt aatttttctc tatttggtaa tgtaaatacc tgtttttgca
    13321 cttcataaga aactaaaact ttttctaaat tctttcttat tataatgaag ttttgttcca
    13381 ttatcttctt acttgccttt tctataataa attccatatg tacagaaggt aataaaattt
    13441 ccccagttcc atgttctgta tatttactta aagcatcaat tatatctaag tttcctttta
    13501 tcttattttg taatttcatg caactatctt gtaagaataa tccactttgt ttggctaata
    13561 cgttgataaa actctctata gtaagacttg ttgtactttc caaccagttt ttaaatactt
    13621 ttacaacctc atcatatttt gctatttctt gaatcattgt tatacttcca ataatattga
    13681 taaaatcttc tgtatacgcc ttaacataat cttctaggct agatacgata ttgttttgaa
    13741 tttcttttat caacgtatct aaaactaatc ttgcatctgc actaattgga tattggaatg
    13801 ctgatgtctt atctttaact tcatctaatt ctagtacttg ctttaataag ccttccctct
    13861 catctgatgc aagtggctta caaagatgaa tattttttcc attatctttt agcaattctt
    13921 caaataacca agctttttcc tctatattac tgtctaaatt ttcgcataat tgttcatatt
    13981 ttctttttat aacattatat atttgttttt ctgttcttcc taagaaactt tttgaaatga
    14041 aaaaagttat actaccctta aaattttctt tatgaagaca catatttaac tgttggaaca
    14101 ggtctagtac ttttgtaagt ctagaatcta atatatctgc ttctggtatc aatacaatta
    14161 ctgcacatat agatgcagaa tttttaaata cttcacctaa tgaaactgct gcactaatat
    14221 catgggctac tcccctattg tcgctaaaac ctgcagtatc acataatttt gcatccattc
    14281 cttctattgg ttttctcata tttacaatgt tcgggtataa agtttgagaa acagctcctg
    14341 aaccaatctc tgccacaggg tctaagcaat caatatatga tataccagta tcttcatctt
    14401 ctacttccat cattacacat cctgcaagat agttaattgc agtacttttg ccacatcctg
    14461 tcccaccaag aattagaatt aaattttgat tttcagcctt atttattgct acttttcctt
    14521 catttaataa aagtgttatt tcttctaaaa gttgattgtt attttctggc atttattttc
    14581 ccccttggtt atatatttta tttaaataaa gtttaatccc ttaagtagat tgcaaagttt
    14641 ttagactata tttaaatttg ttttttattt tttattttta ataaatttgc attttcacta
    14701 aataattatt cttctttgtt tattaaaata actgtgttgt ataatataat ctttatcaac
    14761 acagttattt tatttaatgc gcttatttta ttgtatttta tgtaatacag ttgtttttta
    14821 tattatatgt ccattatttt tctatggtta ttacatatag atttttaatt atatttgtac
    14881 cattaattta aacattccta tactactgct taagttttac acctatacca gcttgaatat
    14941 tttattttta tttgcttaaa tagttcaact atactggttt aaataggtta tctttatttg
    15001 cttaaatagt tcaactatac tggttaaata ggttatcttt atttgcttaa atagttcaac
    15061 tatactgatt taaataggtt atctttattt gcttaaataa gttcaactat actgatttaa
    15121 ataggtttcc tttagtttaa ataattcacc tatactggtt taaataggtt tcctttagtt
    15181 taaataattc acctatacct acttgaatag ttcactttta tttgcttaaa ttctatacca
    15241 atactggctt aaacattata ctaccatgat tgtattgtct atcttcaact acaataaaaa
    15301 caggttctaa atctttagta gatgttattt ttaattctaa aaaaatatta tcagttggta
    15361 atatttttac atcataatta tttaaattat tattcgtgat actattaaat acatcacctt
    15421 gacgttcaaa aaaaacatta tcaactatta caaaataaac atcttctttt ctactatttt
    15481 caacctctat agtattttgt ttagttatat ttagtctcat cttccacctc ctattaatat
    15541 tacttaatta tattaatact tgtaattgtt gcattatgtt catcacaagg aagaggttta
    15601 aaaacaacta tctctgctaa ataattctgt ggaggatttg taactgaagt agcattgcaa
    15661 ataaatttaa aatttgttcc tgctacaact tgagtagaaa caataagtgg ttcataacca
    15721 accccttcta acattcctac agcttcatta aatactttta aatcttcttc ttcgacagct
    15781 ctgaattttg accatccacc aacaaaaaga ctcatataaa aacctccttt atatatatat
    15841 ttatttatat atagtagtac caattttctc aatattcaat attttctgtg taactggtca
    15901 atgaaaatgc ataactggat aaaaatactt ataaaataac ttaactttac atataaatag
    15961 cttactttac atgtaataag attattctac aatatgaaat gcatcttgtt ttgaatatat
    16021 tctaaataca ataatggata ttttcaatta ttataattag aaaatatcca ttacttaact
    16081 tgttttattt aaaatcacat agagacttta ttattttttt tattgcatga atagctattt
    16141 caggtatcat aaggtgatat accccctcct tgaaaccaca aaaaacatga caaactttac
    16201 gaatcttgtc ttaaaaaact acaggtacat tccttatttg tggattggag tagccataac
    16261 aagcaaattt tacaattttt atgatgacaa aaatcaataa aatcgcttag tatataacac
    16321 gaaatactac ttgttactat taagaatagt tagataggaa gtatgcatta tacaaataaa
    16381 ggaatcaata aagaaagtta aaacatccct acaaattaaa aatctgttac atttttctat
    16441 atttaaacgt tatttactta caattggaga aataacaggt tttccttctc ggtcaagtaa
    16501 aggcgtaaat ccggctgcat tgccataata gtggaataca tagttcacac ctgtttcttt
    16561 atccacccaa atctccgttg catttatttt cccttgtgaa tatacttttt caaatctatc
    16621 ttttcccatt ttataatcct ccatattttt gattggtcta tgccttcttc acttaagtag
    16681 aaaaaataca taacttattt ttttaattct aatatgagca cgtaaaaatg tcaaacagct
    16741 tatttaattt cataacttca tgttactgtt cgtaaccttg tctgtttact tacactgcat
    16801 tacagcacca ttttatcctt tacattattg tattgcacac agtaaaaatg cttactttat
    16861 gggatttttc aactaaaaaa aggattttat ctccacaaaa catttactat ttgttacaat
    16921 atacctaatt ttaatctcat atcaacattt aatgaagata taaccttttt caaatattgt
    16981 aaagactcct taaaaatatt agaaaaagat tctgcaaatc tctcaacatc tccttttttt
    17041 aaatacctaa gtatctcaaa acatacatca aaacgtgttt tcaaaatttc attatattct
    17101 tcatgaaaaa atctctctaa atagattcca aatatcatat attgttttaa atgcaaaaat
    17161 gtttcctgta atacataaat ttgtgtataa tctactatct tacaaagtaa aacaaaatct
    17221 ggataaacct tacactcttc acaattattt tgtaagtacc ttaaatgagt ttctatttta
    17281 tctacagcta tcaaatccat tttatttcct agtaaaagta ctacatcctc aagagtaatc
    17341 gtaacaaact ccatagcatc caaaagcaaa ctcaaccttt tagctccttc ttctgtattt
    17401 atataatcat catcatattc agaaaaaagc gttacatatg ttcccttgcc attaattgtt
    17461 ttagcaattc ccaattcatt tagctcatta taggcacttc taactgttgg aagtgataca
    17521 ttatatctat taattatcga agtaattgat ggtagacaat ctcctatttt aatattgcct
    17581 tcaaaaattt gacaaattac atctgaaaca attaagtcat ataaataaca tttttccaac
    17641 gttacagaat taaaactact tgtatctata ctttgtggag cacgattttc taccatttta
    17701 agtatggatt ctctacaagc tttatacata tatgttaata gttttttaaa ttcaattaga
    17761 ttcccatcca atccatataa gtaagttttt cttacctgct ctatgtattt attacgaaaa
    17821 aagtctatac aacaattatc caagttatcc aagttatcga aattataggc caattgtgaa
    17881 ataatgacat catgctgaat aatctcacaa aaatggtgaa tcaggaaatt atttgtttta
    17941 cttattagtt tatttatata ttttagcctt tgttctttaa agactaaata attagaacta
    18001 tttttttcca tacaatcgat aatctcgttt aattcttcaa agtctttaga tgtaaatctt
    18061 ttaatattgt acatagcaat agaaggaaat attaaactaa aaatcttata tatttctgat
    18121 atttgtaaaa tcttctcata taataagtca gattctattg attgtttact cgtactctgg
    18181 attaaaactg gtctagaacc agcttttgtc tctataaagc ccttttctga gagcattttt
    18241 attacagttt ttatagtatt ttttgatacc atatattctt ctgacatgct ttttatagaa
    18301 tcaagcctat ctccaatagt aattttacca gtataaattt gttgaattat atgctcaaaa
    18361 ataattaaat attgtggttt tctcttcata tttttcctct tcgataaatt agatttattg
    18421 ttttttgtaa ggtatattta cttttattta atagggcaaa atagttgctt attttttcct
    18481 cataatctat aattaatata aaccatttat atatatggtc taagttttat aatactgtaa
    18541 tgcagtaaaa tagcatgttg tagctatact acagcaaatt aacataccat tgtagtatat
    18601 taattggaat aattttaagt acttcaaaat tccagcaaaa catatatgtg tttttgatat
    18661 tatgtatatt cgttaataga atagctatac ttgttttaca taataaatta tatgatactt
    18721 gctatatgta atcactatat ttaatacaca taactattat acttgctata tgtaattact
    18781 atactttata cacatagtta tcatatatgc tatatgtaat tactatactt tatacacata
    18841 gttatcatat atactatata taattactat acttggacac atagttatca tacatgctat
    18901 atgtaattac tacacttgga cacataac
//
LOCUS       DAELTA010000073        18748 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.073, whole genome shotgun sequence.
ACCESSION   DAELTA010000073 DAELTA010000000
VERSION     DAELTA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.073"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(433..1305)
                     /locus_tag="KPG13_002103"
     CDS             complement(433..1305)
                     /locus_tag="KPG13_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0985819.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1699..2277)
                     /locus_tag="KPG13_002104"
     CDS             complement(1699..2277)
                     /locus_tag="KPG13_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0985820.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2335..3051)
                     /locus_tag="KPG13_002105"
     CDS             complement(2335..3051)
                     /locus_tag="KPG13_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0985821.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3242..4327
                     /locus_tag="KPG13_002106"
     CDS             3242..4327
                     /locus_tag="KPG13_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0985822.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4482..5141)
                     /locus_tag="KPG13_002107"
     CDS             complement(4482..5141)
                     /locus_tag="KPG13_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0985823.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5358..6650)
                     /gene="lysA"
                     /locus_tag="KPG13_002108"
     CDS             complement(5358..6650)
                     /gene="lysA"
                     /locus_tag="KPG13_002108"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG0985824.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6668..7861)
                     /locus_tag="KPG13_002109"
     CDS             complement(6668..7861)
                     /locus_tag="KPG13_002109"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG0985825.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8222..8839)
                     /locus_tag="KPG13_002110"
     CDS             complement(8222..8839)
                     /locus_tag="KPG13_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985826.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9009..10709)
                     /locus_tag="KPG13_002111"
     CDS             complement(9009..10709)
                     /locus_tag="KPG13_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985827.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11106..11669)
                     /locus_tag="KPG13_002112"
     CDS             complement(11106..11669)
                     /locus_tag="KPG13_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985828.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11828..12541)
                     /locus_tag="KPG13_002113"
     CDS             complement(11828..12541)
                     /locus_tag="KPG13_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG0985829.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12833..14497
                     /locus_tag="KPG13_002114"
     CDS             12833..14497
                     /locus_tag="KPG13_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG0985830.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14604..15503)
                     /locus_tag="KPG13_002115"
     CDS             complement(14604..15503)
                     /locus_tag="KPG13_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG0985831.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15530..16168)
                     /locus_tag="KPG13_002116"
     CDS             complement(15530..16168)
                     /locus_tag="KPG13_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0985832.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16505..17503)
                     /locus_tag="KPG13_002117"
     CDS             complement(16505..17503)
                     /locus_tag="KPG13_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG0985833.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17601..18176)
                     /locus_tag="KPG13_002118"
     CDS             complement(17601..18176)
                     /locus_tag="KPG13_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985834.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            18492..>18748
                     /locus_tag="KPG13_002119"
     CDS             18492..>18748
                     /locus_tag="KPG13_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985835.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTL"
BASE COUNT         6119 a         3080 c         1988 g         7561 t
ORIGIN      
        1 acttaaaaaa tcaagatttc atacctaact actaattcta tgatttttaa atttttcaac
       61 ttctttgtca aagttaaaat aaaatactaa aagacacata ttatagtgaa caaaaaagat
      121 agaaatacat attttaatat aattttaata agatattata tacttaaata caaataaaat
      181 taattttata attgttaatt aaggaaatga tattgtttag agaaataaaa aaagcactct
      241 ccatgaatgg aaatgctttc tttaataaat tagttgacgt agtaaagatg tattcagaat
      301 acatctttat gttttgagtc tcgaaatatt tatttgtatt atttaaacga aaatttattt
      361 tttaagtgat aatcgagtat ttactaataa ggctatgatt ctcaatttca tagccttatt
      421 aatatacaaa ctttactcat acacagaata tcttttccaa atttgctcta aatcatcaaa
      481 atgctgttta atatcttctt tttccttcat cccaacagat atagcaaggg catttgaaat
      541 actaagagct ggaactagag aatctacaaa tgaagccata ttactcttaa ctagaagagt
      601 gttatctgca agtgatgcta ctggcgcaaa taagctatct gttaatgata ttacatgggc
      661 tcctttttct ttagcataat tgacaatttg ataagatttc ttagaataac gagggaaact
      721 aatagcaact attacatctt cttcatttat tcttacaatc tgctcaaaag catctcccat
      781 atccattcta attatatgaa cattgtcaag tattatatct aagtaaaaac ctaaatattg
      841 agctactaca aaagaactcc tcattccaag tatatatatt tttctcgctc ttaaaagttt
      901 gttagaagct tctttaaacg ctctctcgtc aatttcttct agtgtagacc ttatattatc
      961 tatatcactt tttaaaactt tgtttagtat tgcaaaatca tcggaataat catgtgccat
     1021 ttcaactctt tgtactgtag ttagtttatt ttttatgagt tcttgaagtg cagcttgtaa
     1081 tttaggatat ccactataac ctaatgcatt tgcaaatctt acaactgtag actcacttac
     1141 tcctacggtt tcacccaatt tacatgcagt cataaatgca actttatcat aattatttaa
     1201 aatgtattgt gctataagtt tttgaccctt actcaatctc gtatattgag attgtatatt
     1261 tgatatcaaa tgtttactgt ccttcatttc atttgtttct tccatttttt tctcctttta
     1321 gttatataaa ctattttgtt ttattaacac tgtctttttc tctaaaaatt ttatttttta
     1381 tctcaatttc atcttagttt ttatcatagc ttcttaaact cataaaattc taacttttct
     1441 gtaccttata cttcacatag tattttcgat taaatttatt ataccatctt tccttgcatt
     1501 ttttaatatt tatccaatat ttcttttaaa tttttcaatt tttactcatt taaaagttat
     1561 ctatcttatt ttgcatattt agatgtatat tttgttttga gaataaactg ttgtatactt
     1621 attatttaaa gtatttttaa taggtcacac tatatatatt aactcttaat acaatcttgt
     1681 caagtttttt agtatttatt atgctttttt cttttcaatt aaaatcaatt gtggtgggtt
     1741 attgacttga tttaaaaact tagattctaa aacattaaat tcatcttgat ttaaattatt
     1801 gacaaggtta tatatgcaat ctttttcttc ttgacctcca tcatgccctg tatatatggc
     1861 aatgcttatt actccatttt cttttaacat ttctaaactt aactttatag cctttaaagt
     1921 tgtatctggt ttggttataa ttgaatgttt cgctcttgga agataaccca aattaaataa
     1981 aacacatgaa acttcttctt ttatatattg attcattttt tcatgcccat caaatatcaa
     2041 ttttactcta tctgtatatc cttctttctc tagtttcttt cttgtagact ttattgcttc
     2101 ttcttgtata tcaaaagcat atacaaagcc cttttctcca accttctcag ctagatacct
     2161 tgtatcatac ccatttccca tagtagcatc aattactatg tcaccttcat ttataatatt
     2221 ttctaaataa aatttattta tatctgtaat tttagttaaa tactttgcat atgccataat
     2281 actatttctc cctactatta actttatttc attttttctc aatttatttt tatcttattt
     2341 tattgatttt aaatatttta tttcatcttc tgttaggtat ctatattctc caactttggt
     2401 gtcttttaat actattttcc ccacagaaat ccttcttaaa tttaaaactg gatgatttat
     2461 agctttgcac atttttctta cttgtctatt tctaccctca tgtatagtta tttcacacac
     2521 tgaataattt ttttctttat tttctttagt tacccttatt ttggcaggtg ccgttgtgta
     2581 atcttctatt tttagtcctg tttcgaattg tcttatctca tcatcactta taattccctt
     2641 cacacttgcc acatacattt tatcaatttc atgctttgga tgggtaagct tatatgttaa
     2701 atcgccatca ttagtaagta ttaataaacc actagtttca taatctaatc tccctattgg
     2761 atatactctt tcccttacat ctttaagtat gtctattaca ctgggtctat tgaattggtc
     2821 ttttactgta gttatatatc cctctggttt atttaatact atatatatat aattttcact
     2881 tggttttact tttttattat caaattctac tatgtctttt tcttcatcaa tattaaaaga
     2941 taactctttt actacacttc catttacctt tattcttcct ccaagaatta tttcttctgc
     3001 ttttcttctt gatgctattc cacaagaagc tatatactta ttaattctca tacttaatcc
     3061 ttccttatct taaattatgt gtgtttaata tttttatcaa tgttaatttt gctacattat
     3121 tatactatac tagaccttaa ttaaaaacaa gcaaaaaaat tatttaattt tcctaaataa
     3181 ttgtaatttc ttttcacata ctattaatat gtaatagctg actaaacact attttaagga
     3241 agtgattatt atttcaaagg aaaatataaa atattattta atagtcgtgt ttattggaat
     3301 tttatttttt aaatttatta atactccatc agactttata tctagtattg aaggattctt
     3361 aaagtttttt agcccatttt tattagctat tttacttgct ttacttttaa atccacttgt
     3421 gatgcttttt gagataaaat tcaaagcaca tagactttta gccatttttt tatcttatat
     3481 atttataggt tttattttag cctttgccat taggttatta attccatcaa tagctaatac
     3541 attaaataga ttgataaatg aaatgcctat gtatactgat tatatagata gttttataga
     3601 aaaaaatatg tcaaacatat attttctaaa aactttaatt cctcatatac aacatagttt
     3661 agataattta ttaaaagaag caagtaattt tgttggtaaa attcctaaaa acttcttaat
     3721 ctatacactt agtatatctt ctatgctatt taatatgaca atgggattta tactatctat
     3781 atacattata tatgacaaag aaaaaattgc attgggattt aagagatttc tatattcttc
     3841 tactgctaga aacaaggcag ataacattat agaatttttt aggactactc atgatatttt
     3901 ctatgattat ttacttggta gaatattaga ctctcttatt attggtataa ttgcattttt
     3961 aggatttcaa tttgttatca gaatagaaaa tgctttattt ttagcttcaa taatattctt
     4021 aggtaatata attccctatt ttggtccttt tattggtgca atccctccaa tagccatgac
     4081 tatattatat agtccacaaa aaactatatg ggtcattgca tttctattta tattgcaaca
     4141 attagatgga aattttatag aaccaaaagt tatgggtaat caagttggta taggagctat
     4201 atgggtaata tctgctattt tggtgggaca atctctattt ggtttcatag gagtttttct
     4261 ttcagtacca atagctgctg ttgtaaaaac ttatgttgat aaatatatag ataatcgttt
     4321 acaatagatt atctctactg tgactttaat ttttagttta aaatttaact gctatttatt
     4381 aaattatttc tcaaaaaata tctcttagta agaaaaaatt gctaagagat attttttata
     4441 atatataatt tgttcattaa atttatgcat atattttata cttattgatt atttgagttt
     4501 tgagaatttc caccactatc tgaaccacca gtagtatctt gtggagttgg tgctggtgtt
     4561 ggattactat tatttggctt ttcttgattt tgagaagaac tgttattatt gtcagactta
     4621 ttactatctg attttttatc attgttttta gaactagatt tattattttt actacttcca
     4681 ctataagaag atttccttgc atctgattca actctatacc tatcttctcc agccgtatct
     4741 tttgcctttt tctttctttg ttcctctgct tcgttttgtt ctttttctaa tttagttaac
     4801 tcttctttct ttttagttgc ctcttcttta ataacagagg aaccattttt gattttctct
     4861 ataagtttaa gttccttgat tgcaccctca taatttttta attcttcaaa ctccgtcata
     4921 tttgacattc tcatagcttg catatacata gctcttgcat tctcattagt actatcttct
     4981 tccagtgcag aggataatga ttccatagct tttttatagt tatgtttttc taaatatgtt
     5041 tttccttgct ctactaaatt tatttcatct tttttattaa atgaacatcc tacaagaaat
     5101 atagataata tcgatattaa taaaattact cttcctctca ttaaatccct ccttgtcata
     5161 tactttaaat tttatcataa ttataaagtt tttaaaatat tttatattta ttacaagaaa
     5221 tttgttagta aaacaaaaaa taagcagata tgctacgaac gatacaataa accaaaaatt
     5281 aatttagaga tacatatcct agcaaatctg ctcataataa actttaatat ttaattttta
     5341 ttatatactt ctctatttta tatctctaaa gctaataagt cctcatagct ttgtctctta
     5401 caaattatct tatcatttcc atcaaatact gataccactg ctgctcttgg tattttatta
     5461 taatttgaag acattgaata tccatatgca ccagttgatg ttgttataag tatatctcca
     5521 cttttaatat ccattatgtt tatatctcca attaatatat ctccactctc acaacatttt
     5581 ccagctattg ttacatgatt catagcctca tgattaattt tattcacaat actacactca
     5641 taactagctt ggtacaacga aggtcttaca ttatctgtca tacctccatc tacacttaca
     5701 taaaccctaa catcttttat atcttttata gaacctactg tatacaatgt acttcctgca
     5761 tttgcaacta ttgaccttcc tggttctatt accaatgtag gtacttctat tccaagctct
     5821 ttacatgaag tttctgcttt agtgattatg gtttcacaaa actcttttat agttttaggt
     5881 ttatctcctt cgctataata aacgccaact cctccaccta ggtcaatttc tgttagttgt
     5941 atgttgaatt tttcttttat ttcttttacc aagttcatca ttatatccac tgtatctatg
     6001 tatggttcta catcaaatat ttgtgaacct atatgagcgt gaagacctac aagtttaata
     6061 tttttatatt cacttaattt ttctattgct ctaaataaat ctccatttac taaagcaaat
     6121 ccaaatttag aatctatctg tccagttttt atataatcat gtgtatgagc ttctatacca
     6181 ggagttattc taaagtatat ctcttgagtt ttacctttat cttcacataa tgcttcaagc
     6241 aaatctaatt cataaaaatt atccactaca aatttaccta cacctaaatc taatcccatg
     6301 tttatttctt ctttagtctt attgtttcca tgaaagaaaa ctttttccat aggaaaattt
     6361 gatttataag ctgtatataa ctcgcctcct gaaactacat ctaaacacat ttgttcttca
     6421 tttataagat tacacatgta aagaggtaaa aaagctttcc ctgcataagc aaccttattt
     6481 tttccctctc tagccttaaa atattctata tattctctac aattttgtct aactaaagct
     6541 tcatcaaata cataaagtgg agtttgatat tttttactaa gttctaaaca actcactcct
     6601 ccaatatata attcatcatt atttacagtc atagttccat gaagtttcat aatagcctcc
     6661 ccaaatttta tatattaaat acttctgcta ttttttctat agctacttgc ttatcatcag
     6721 aatttatagc acaagttata cgaatctctg atgtagttat aagttttatc attataccat
     6781 tttcattgaa tatcttaaat actttagcag caactccaga agtatttttc ataccaagac
     6841 ctactaaaga aaatttagtg atattattat caatcactaa atgttcttca tctgtatatt
     6901 tagatactat ctttttacat tctcccaact cttcttttgg aactgtaaat gatacactta
     6961 tcttatctaa aataggtgca gtttggctta tcatatctac acttatacct atagttgcta
     7021 tatcttcaaa taagctagat atattttcag ctttaaaatc ttttattgtt atagaactgt
     7081 catcatcact agtagctaat cctgttatta ctttatcctc taatttcatg tcttttcccc
     7141 ctcttatata tgttcctttt tctgtaccac atgtacgtcc gacatagatt tcaacaccat
     7201 atttttgtgc aagctctata cttcttgaat gcattacttg tgctcctaaa ctcgctaact
     7261 caagcatttc ttcatattca atctcatcaa gtttactagc ctttgaatat tttcttgggt
     7321 ctgtaaaata aattccatca acatctgtat atatctcaca ttttccattt aatttaaccg
     7381 caagagctac agcagatgta tctgaaccac ctcttccaag agtggttaca tcaccatctt
     7441 cgttgattcc ctgaaatcca gttactatca cgacatttcc ttcatctaaa ctatttttaa
     7501 ttctgtttac gtttatatca tcaatttgag actttccatg aagaccactc gtttttatat
     7561 ttaactggta agcattataa cttatagctt tacatccaag tgcatttaat gctattgata
     7621 ataaagatgc tgaaatcatt tcaccagtag acattaaagc atctaattcc ctcttactag
     7681 gttcattact caattccttt gctaaagtta tatattcatc agtggatttt cccatggcag
     7741 atacaactat taccatttgt ggattttctt ttcttcgttc tattatattt tctgctattg
     7801 attttatttt ttcagtatct gctactgaac ttccaccata tttttgaaca attatactca
     7861 tgctttctcc ccctcaagct aaattttgat aaataaaaac gcccaaaggt atactttgga
     7921 cgtgtaaatt tcatagacca agtaaccttg tagctcacca ctttaaaagt gacagcctta
     7981 tgcatattct gcataagtcc agaaaatttg tccgtcaaca aaatttcttc ggctatatac
     8041 cctttcatca attatcaacg cttaccagct cctattgaat actattgaat acagctacct
     8101 ctaccctagg ctcattattt ttaattaatt tattaatata gttttatcat attctatata
     8161 atattgcaac aattatttta caaaatgtgg caaatcgata gttttctttt ctaaagttag
     8221 cttatataca attaaatagt ttatttaatc ctaaatctat aaaatttatg ctatactcat
     8281 tatcatatct ctctaaatca tttaattcgg atagattatc tggtgtaact accaaaacgt
     8341 ttggtacttt aaaaaatgga attgtagatt tatctatttt tttagaaaga ttttcgtatt
     8401 tagaaatgtc tatttcatta tttaagtcaa catcaactaa aacttgcttt atattaccaa
     8461 attggtctgt atattcaata aataaatcgt atttcacatc atctacacta acatttcggt
     8521 aaaatctttt tacatcacaa cctgcagtat tgattctaac aactaattct gatcctacta
     8581 gagcctttat taaatcatct cccttaaaaa gtttactacc gtttaaatag tatacataag
     8641 agaggtcttt ataattataa taatccacat cacaaggata tttttttagt gtatattcac
     8701 tacacatttt tctcaaagtt tttttaaaat tttttttatt gtatcttttt tggtctccat
     8761 aaatttttct aaattgagac tctgtaacac atctaaccct gctaattaaa agtaatatct
     8821 ctttttccat attcgtcata gtacattctc cttgaagtat tatttgcaaa gttgtactaa
     8881 tattatatta attttatgac attaaattta caaagaatta aaataaaata gctaattttt
     8941 atagaattaa attctataaa aattagctat cactatgtta aatactatta tcaatctttt
     9001 tatttaaatt atacattacc ttgattttca gaatctttgg aatctgtatt ttcatcagtt
     9061 cctgtttttt cttttgcttt tttatcttca aagtttgcaa tatttaggtt atcaaattct
     9121 gacatttcta ttacaccaga ttcttctttt gaatacgata ttttgacttt atcaccaacc
     9181 tgagtcaatg gcaattcgct agatactttt gaagtagccc taaatattat atctttattt
     9241 gaatctaaag ttacataata attagtgtta ccacctcgca catctgggct tattcttgtc
     9301 actgtgcctt ctaatacctc atcttttaaa tcattatcaa gttttaaatc attaccctta
     9361 gattctaatg catccttata gtttcttaat gcttcattct tatcttcacc tattccaagt
     9421 acattataat cttcaacaga tacaaatgca actctcttaa caagacccgc cttatctttt
     9481 aatgatgata catatgttgg ctgacccaat atattataca ttacagggaa cgtcgcttca
     9541 taattttttt cttgaacatt accttcagct gatttcattg cagccgtttc agtagctcct
     9601 ggttgtttat ataatctagc ttcttttgtt ctagaatcaa ctaacataaa tccaattgta
     9661 gattcatctc cacctgaaga tgttatacct gtataccaat aagacctgtt atcattgcca
     9721 taaactaatg atgtaccttc tgttggtact aaaactccct tttctgatat aacagagttt
     9781 aaaaatccat taacataaac accccagtct ttaatttggt ctgttacaaa gctttgtggt
     9841 tgtattctat ccacccattt aggcgtgttt ttaacatcat aaacatttat ttttccagtt
     9901 tctgcatcaa cagtggctat tcctgttgca tttgctcctc catagccaat tttatgttca
     9961 tataaactta caacccaata aggtcttcct tcatcattta tttcaagagt gaaatctgtc
    10021 ataccaacat ttacaatacc atgtatgtat aaatgtcttt gtaaatcctg atgtaagtag
    10081 gcttctggtt ggtaaactat ctttattggc tttccatcta tttcttgaac taattggacg
    10141 tcttgaggat tactagctga tacttttaca taaccacttg taccacttag agatgttatc
    10201 catttaatta tgtctctgtg aacaagaggc gctacccagt acaactctcc atctacattt
    10261 tgtatatgaa attttcctaa tttggataca ctccctattg caggaacttc tccaagcttt
    10321 ttgtcaccta atttcattgc catatcttca tctacaagac gaatgtcatt tacactgaca
    10381 gggctaatgt catcagtaaa tttactttca gttacttttc ccaatagttc tctatatgat
    10441 tttgcatgaa gtacaggtgt agaagttata aagggaacta ctacaatata cacaagcaaa
    10501 gctattgcta ttgaaaaatt atatttagcc attttagatg ctctttcatt tctatctaac
    10561 atcatatcta gtattcctgc tacaacaaag aaaattattc ctactgaaaa taaagatgta
    10621 aaatctaatc ttagtacagg taatttaata aatgcataaa ttattacaat tattaatgca
    10681 actccatatg ttttaagata tcgtttcatt taatactcct ctctaaaaaa tgttattatt
    10741 actattatat ccatatattt acaaaaaata aatatttatc atatttatta tatattaaga
    10801 tatctttata tatttatagt tctgcttatt caactatatt taacattata cgtgtatgta
    10861 taaatacttt ttatacactg tttgtgctat ccatcccgca cctaaaggag tgggattttt
    10921 gcactattct gtattatata tatccaaaaa tgttataaaa atacaaatat tagcagaaat
    10981 ttaaaaactg aagaaagtat tctaactaag atttacaaat tcttaatcct atcgaagtta
    11041 aactactatc ttcagcctaa ttatctagca atattttttt atatgaaaaa ctctaaatca
    11101 agtttttaaa tgtttgcgat ttttgataca taagaatgtt gtctagaatt atattctgat
    11161 aataaacctt catactctaa ggttaagcca gataaattat aaaaatcatt tggcgttact
    11221 tcaaatgctt gtttatttaa aatgtctatc tcactttttt ctaacatgtc agcaacaaaa
    11281 tttgaacata catatttata ttcagattgt ctctgaatat tcattagtaa atatattaat
    11341 gccattgtat cgtaatagta atcttctcta taatcactaa ttaattttat atttttatat
    11401 agattttcat attgtaaatt atctacttct aacgaatata ccctacacac agtgtttttt
    11461 cttatagcat ataatccttg atttatattt tcttcaacaa atcctccaat aaatggattc
    11521 cttggattta gccttccaaa agaatacatt ggttttagat tttcatttaa tgatattgac
    11581 acatgtgtat acagcttttt acttatattc cttattaagt aagataatat agttcctgta
    11641 tatgttgtta caatatagac tttattcatc atttcccccc aatctagtaa ctaaataata
    11701 ttatacactt acaatttaat atcataagat attctaacat aataaatgtg gttttacaaa
    11761 ttttgatttt aaggtacttt aatattatat aatataataa aaatataaat ataagttttc
    11821 tatattttta accaacagat gttttcttca aattaatcag atgatttagt atatcttgaa
    11881 cctttccaga gtcctttaaa tatcctaatg ccccatttat ttgtgatttt aaatctattt
    11941 ctacttccat atccaaatca acatttcctt tttttataga tatattttta agtgttattg
    12001 ggtaaagata acttttttcc aaagtaatct tatttccatt aacctcaaat ggaacttttt
    12061 tattgtagtt acttaatatt ttttctagca ttttatcact tagtttaaat tttccaattt
    12121 tcacattctc aagacttaca actaagtttc catcctttaa agtaggtctc aattcaagtt
    12181 catattgact atttaccaaa ccaaatacct tatatggtcc tgctaccttt attttaccat
    12241 ttttcatttc aacaaaatta ttttcaagtt cattaattcc atgcttctgc atcaatgtat
    12301 atattaaatt tttaaactct tcctctgaaa cagatatttt tccaaccatt ctaagtggat
    12361 tttttactac ctccatagaa tttatatatg agccttttga taaatattct tcatctatga
    12421 ttttattatt ttgtgaacta acatcatatc tttcttttgg tgtcaagata tatacaacaa
    12481 ttcctgcaag tactaaaaca ataacaatta aacttaacaa aatctttgca acctttccca
    12541 taatcgtctc ctttacaaat ctgtcatcag atttaaaata aatttagata actaacttca
    12601 cataatattc ttaacaatat acatactata atacagtctt ttaaaaatta attcaaccaa
    12661 tctaatgttt tgtaaataaa ctgtaaaaac atgtttttag tataaaagtt aatattttgc
    12721 ataatactct taattacacc tttatttttt tctaatcttg gattaaaatt aatattatgc
    12781 aatacagtgt agtgttctgt attataatca aattaaatag gaggtataga atatgtcaaa
    12841 tgaaacgaac tcatctaact ttataaagaa tattattata aacgacttag aaacaggaaa
    12901 acatgacagt atcataactc gttttccacc tgagccaaac ggatatttac atattggtca
    12961 tgcaaaaagt atatgtctta attttggatt agctaaggag tttaatggaa aagctaattt
    13021 aagatttgat gatacaaacc cattaaaaga ggatgttgaa tatgtagagt ccataaaaga
    13081 agatgtaaag tggttaggat ttgattggaa cgagttaaat tttgcatcta actactttga
    13141 tgaaatgtat aagagagctt taatactaat aaaaaagggt aaagcttatg tatgtgattt
    13201 gacacaagag gaaatgagag aatatcgtgg tactctaact gaaccaggaa aagaaagtcc
    13261 tcatagaaac agaactattg aagaaaacct tgatttattt gaaagaatga aaaatggtga
    13321 atttaaagat ggtgaaaaaa cattaagagc caaaatagat atgtcttctc caaatataaa
    13381 ccttagagac ccaattatat atagaatatc tcattcaaca caccataata caggagataa
    13441 atggtgcata tatcctatgt atgcttttgc tcatccaatt gaagatgcaa ttgaaggaat
    13501 aactcactct atatgtactt tagagtttga agaccaaaga cctctttatg attggtttgt
    13561 taaagaatgt gagatggaaa atatacctag acaaatagaa tttgctagac ttaatataaa
    13621 taatactgtc atgagtaaaa ggaaattaaa acaacttgta gatgagggta ttgttgatgg
    13681 ttgggatgac cctcgtgttc ctacaatatc cggaatcaga cgtaagggat atactgcaga
    13741 agcattgcgc aatttctgta gtgaaatagg ggtatcaaaa gttaactcta cggtagatag
    13801 tcaaatgctt gattatttcc taagagaaaa cttacaacca aaggctcctc ttactatggg
    13861 agttttaaga cctttaaaac ttattataac taattaccca gaagataaga ttgagatgct
    13921 tgaaatagag aataatgcta aagatgaatc tcaaggaaag agattagttc ctttctctag
    13981 agaattgtat atagaacaag atgattttat ggaagaacct gttaagaaat attttagatt
    14041 tttcccagga aatgaagtcc gtttaaaagg tgcttatttt gtaaaatgta ctgatgtaat
    14101 taaggatgaa aatggaaatg tagttgaaat acatggtact tatgaccctg aaactaagag
    14161 tggctctggc tttacaggac gtaaggtaaa atctacaata cattgggttg atgctaaatc
    14221 agctatacca tgtgaattta gattatttga accattaatt cttgatgata tacctgaaaa
    14281 tgaaggtaaa catttcttaa aacaaataaa tcctaattca ttggaaatat tacaaggttt
    14341 tgttgaacct acacagataa aagatgctaa accttttgat aaattccaat ttgttagaaa
    14401 tggattcttt agtatagata ataaatatac aactgatgaa aaatttgtat ttaatagaat
    14461 agttcctcta aaaagttctt ttaaaccagg taaataatct tacaatcaaa aaatgaagac
    14521 ttaaaatata aattacttct actataaata aacttagtta gaagtaattt tttatttgag
    14581 tcttcatcat ttactttaaa tgattaactt ttttcactac agattcccat gacttctatt
    14641 ccatctttta tgcattctat gttaagagga aaatctggtc ctttttctcc gtcaatatct
    14701 gttataagtg aatcttcaca ttctatatgc acctttttag ttttaaagta tagtatttca
    14761 tttccattat attgatctaa ataatcacct ctaagcacac ttatcaaaac tggtattgat
    14821 tttggtattg gcattccttt aaaaataatt acatccaaat atccatcatc tatttcagcc
    14881 ttatatgcta gatttatatt tcctgctgtt ttgccattaa aaatcaacat aagatacata
    14941 tctccatcat aatacatttc ctcagaagat acttttatct taaatttcct catatacaat
    15001 gcttcttcta ttccctttat ataataagaa attcttccaa atgaattttt taagtctgtg
    15061 tttatccttt gtgatacatc cgtaaacata ccagcacttg caacatttat aaagtactta
    15121 ttattgactt tccctatgtc tattttcttt ggttttgaat ttatgatttc atttatagct
    15181 tcttttatat caaatgaaat ttgaagagca tttgaaaaat catttgcagt tcctgtaggc
    15241 aaaattccaa ttggaatttt tatgtctaat tctttcattg catttactac taaatctaca
    15301 gtaccatcac caccagctat caatatgtgt tcataagaac tatcaacttc cttaaatgca
    15361 ttctttacag gttctttaat agttaatcta tagggtatca ataaataatt tttttcttga
    15421 tataattcta tgatagtgtc taaatttgat aatatctttt tttcacctga attaggattg
    15481 tatataagtt ttactttttt cattatttct ccttactcat tttattcttt tagtatccaa
    15541 aatcttcatt taaaaaaatc acatgtattc tttctggtgt taattgtctt tttataagtg
    15601 tataactaca gcatattctt cctggacttg aacagtctat acatatacca gtagtcttac
    15661 atccagtatt tgtacttaat cttttacaat tggctggagc agctattgtt tttgttcttt
    15721 ttatagcttc atctacatct ttaactattt tatttactcc tgctataact attactttat
    15781 caggtccata tatcatagca gcaactctat tcccattccc atctaaattg taaagttctc
    15841 catcctctgt tatcgcatta gtacttgtaa aataagcatc tgcagaaaat gactttctat
    15901 atatttcttt catttcttct ggtttgatat cttttgcata tctatcaaga aaattatatt
    15961 tttctcttat aaaatcaatc actcctgttt caaataaggt catagaacct ccacatccta
    16021 ctagagaacc tgctttaact atttcttcaa tttttttaag aagctcttct ttgttactta
    16081 ctaaataccc tttcatatta ttgttttcca aagattttat tgttttttct attctttttt
    16141 cattaagcca atttaaattt tgatccattt taacttcccc cttattttta atatgcactt
    16201 aattatttat aaactttatt ttctttataa gtttatttta gtacatctaa aaaaaaatac
    16261 aatataaaac ttaaatagtt ttacataatt ttttccaaaa taacttactc aatcaaaaaa
    16321 ggaaagtaga tgtactattc tactttccct atattgtatg tagtatttat tgcctaaaat
    16381 ctatatatta attatatgtt tcatattata taatatcaca gttaattaaa atgtaatact
    16441 atcttctttt taaacatata aattgtatta ttaaatttaa tatatccaac ttcaataaat
    16501 cttattaatt tgatgaagaa gatgctatag ctgcacttgt tgctgccatt acacatgcaa
    16561 tttgaatagc aacaacaata tttaaaaata tattattagt tatagagcct atattattct
    16621 catttcgtat caaatctgca taaattgaag ctacaatagc agaactaatc attactctat
    16681 tactttttcc taaagaccaa tttccaaagc ctttttcttc tttaagttta tttgaaacat
    16741 tttttatctc ttctattatt tcatcttctc tataatctaa taatgaaatt gctcctatta
    16801 ttggatatcc ataataattc attttacaat cattttcttt aaatgctttt ttaagttcaa
    16861 tagtcttttt acatttaagc atagttctat catcactaaa taaaagaatt tgggataatg
    16921 cttgtaaatc atttccttta taaaaaccat tttcatttag aaattcataa catttttcta
    16981 tgtattctaa atcttcatct aaattttttg aatgaattgc aataagtgct atattacaat
    17041 agtcatcact agaagttaga aatggatgtt ttttcctcat gtaatcatat attattttca
    17101 tttttgatat acatgtaaga taatccattt tctctctatt ttcataaact attgatgctg
    17161 taagtggtaa atatgtatcg ccccaaaact tttcactttt aagttctttg tatatatcta
    17221 aaatcatctt aaaactactt tctggatttg attcaaacga aagtagtgtt gccatataaa
    17281 acatattata tcctctaaaa ttagagaata atccagtatt ttttttaata agctctattc
    17341 gttcttccac taatctagga cttattctct tgttcttaag ggtaaatgca agtgcacaac
    17401 tatgttgtat caatcccatt tgccaagaac ctttaacttc atttaatgct ttgtaatttt
    17461 ctattaataa gtttactttt ccatttataa acgattgttc catattgccc tcctagtaat
    17521 atatctcatt gtactaatag ttttatattt attatatata tattaaatac tacaatcaat
    17581 acatattact tcaaagaaca tcattcttca acaataatgt agcctttttt gtaatgtctt
    17641 cccatattat tgactctatc atagtactct gattcacttt cattactcca tttgggcaca
    17701 aatacatcta ttgcattaga attactaaaa tacttactag accccctatc cataacagtt
    17761 tttaattttc catctacaat aatcttagtt ccaaaactta agtgattgtt tgctacaaaa
    17821 ccatcttcta aatcatctcc tgtagcagtt atcgctccat atccattttc ttgattaaga
    17881 cttgtgtaat aactaacttc aaaagttata ggtactaact tctttatttt aatacctaac
    17941 tgattttgta gtttctcctt ttgtactttc aacttatact catcactatt tttataatct
    18001 tccatacctt gattatactt ggaattaaag cttttacctg actttactat agtatctata
    18061 tttactatag attctttaca atgatctgca tctatttttg tttctccatt taccaataat
    18121 ggagttaaac atacactaca tacaacacat gtaatcaatg cttctttaag cttcataaat
    18181 tcccccctta ttattttgat atattagcat atttaatatt atatgtctac atttagattt
    18241 aaataccaca cccatttgct ttaagaagtt gtcttatatt taattcacct taaacactta
    18301 taaatcctct atcttagaat catatagaat aaagctttta tctttaaata ttttttatta
    18361 atcttttaaa atctaaaact actttatcta attttctctt ttttagaatt taagaattaa
    18421 aattctataa ttttaaaaca tattgaattt atttttttga gatgatataa ttttcttaag
    18481 aggtggttga tatgataaaa aatacaaaac aaattacagt agaagttaat gaaattttat
    18541 tacaagaaat gtctaagata actaatgacg aaaataatat tttgacagat ttgataaatg
    18601 aatcaattgt aaattatatt cttgaaaaca aaaaacataa tattgaaaat aaagatttag
    18661 aagaagtgat taaattttac aaaaaagaaa atactgaaaa acctaaaagt gcagcaagag
    18721 ctatagctga agcaaaaact catactct
//
LOCUS       DAELTA010000074        18656 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.074, whole genome shotgun sequence.
ACCESSION   DAELTA010000074 DAELTA010000000
VERSION     DAELTA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.074"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(211..1572)
                     /gene="rlmD"
                     /locus_tag="KPG13_002120"
     CDS             complement(211..1572)
                     /gene="rlmD"
                     /locus_tag="KPG13_002120"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0985836.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1599..2321)
                     /locus_tag="KPG13_002121"
     CDS             complement(1599..2321)
                     /locus_tag="KPG13_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG0985837.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2321..2650)
                     /locus_tag="KPG13_002122"
     CDS             complement(2321..2650)
                     /locus_tag="KPG13_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG0985838.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2980..3906
                     /gene="fba"
                     /locus_tag="KPG13_002123"
     CDS             2980..3906
                     /gene="fba"
                     /locus_tag="KPG13_002123"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG0985839.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(4041..5672)
                     /locus_tag="KPG13_002124"
     CDS             complement(4041..5672)
                     /locus_tag="KPG13_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985840.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6155..7192)
                     /locus_tag="KPG13_002125"
     CDS             complement(6155..7192)
                     /locus_tag="KPG13_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG0985841.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7211..7996)
                     /locus_tag="KPG13_002126"
     CDS             complement(7211..7996)
                     /locus_tag="KPG13_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0985842.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(8029..9162)
                     /locus_tag="KPG13_002127"
     CDS             complement(8029..9162)
                     /locus_tag="KPG13_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG0985843.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9210..10337)
                     /gene="hadC"
                     /locus_tag="KPG13_002128"
     CDS             complement(9210..10337)
                     /gene="hadC"
                     /locus_tag="KPG13_002128"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG0985844.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10337..11563)
                     /gene="hadB"
                     /locus_tag="KPG13_002129"
     CDS             complement(10337..11563)
                     /gene="hadB"
                     /locus_tag="KPG13_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG0985845.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11578..12378)
                     /gene="hadI"
                     /locus_tag="KPG13_002130"
     CDS             complement(11578..12378)
                     /gene="hadI"
                     /locus_tag="KPG13_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG0985846.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12417..13616)
                     /gene="hadA"
                     /locus_tag="KPG13_002131"
     CDS             complement(12417..13616)
                     /gene="hadA"
                     /locus_tag="KPG13_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG0985847.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14293..15291
                     /locus_tag="KPG13_002132"
     CDS             14293..15291
                     /locus_tag="KPG13_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG0985848.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15653..16180
                     /locus_tag="KPG13_002133"
     CDS             15653..16180
                     /locus_tag="KPG13_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG0985849.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16207..16803
                     /locus_tag="KPG13_002134"
     CDS             16207..16803
                     /locus_tag="KPG13_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG0985850.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16868..18451)
                     /gene="asnB"
                     /locus_tag="KPG13_002135"
     CDS             complement(16868..18451)
                     /gene="asnB"
                     /locus_tag="KPG13_002135"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG0985851.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5805 a         3421 c         2289 g         7141 t
ORIGIN      
        1 tatattttca cataaaaaat agatattaaa tttataaatt taatatctat ttctataatt
       61 aaaacttttt ttatttcgta ttcccacact ccatatatat aactacagac acatcaaaac
      121 accttttcaa ttctttattc ctaattttat taccctctaa tctaaatatt atatgtctat
      181 gaaactgtct aaagctcaag atttctagca ttattttcga cgtagcaaca ctacagtctc
      241 acaatgtggc gtatttggga acatatccat acacttaacc atcttaatct cataaccata
      301 ccccttaaaa tcaaccaaat caacaaccaa agacttagga ttacaagaaa tataaacaat
      361 ctccttagca ccaaatccac aaatatccct aatagcatcc ttatgaatgc ctggtctagg
      421 agggtcaaca ataatcaaat caggcttgtc ctttaaatcc ttaacaacct tagccacatc
      481 cccagcaata aactcacaat tagtaagtcc attcaactta gcattctcat tagcagcaac
      541 aacagcttcc tcaactatct caataccata cactttctta gcagcaccag ccataacctg
      601 tccaatagac ccagtaccac tatacaagtc aaatacaact ttatcattgt aatctccaat
      661 aaaatcccta gcaatactat aaagaacctc tgctccctta gtattggttt ggaaaaacga
      721 aaatggtgaa atcttaaact taagacctaa aatttcttct tgtatataat ctcttccata
      781 caatactctt aactcatcac attggactgc atctgctaaa ccatcattaa tagtatgaag
      841 tacacctact aactcagctt ttaaatcaat ccctaaaagc atatctttaa actcatacat
      901 atcaaaatct tcttgagaag aagttactat attaaccata atctcattag tatgaatccc
      961 ttttctaact acaagatgtc tcaaatatcc tttgtgattc attgttctat aataaggaag
     1021 ctttttttca ttgaaaaact ctacactaga aactaatatc tttgagaagt cttcatcaac
     1081 caacatacac tcctcaacag tttgtatatc tatatgcttt ccttttttat gaagacctaa
     1141 tgtcaaaggt ccatttttta cttcatctcc aaaagtatat tccatcttat ttctataata
     1201 tttgttttct ggacttcctt ctatccctaa aaactgaaat ccaaataaat cttgctttaa
     1261 aaataaatcc ataacttgtt tttcttttat ttctaattgt ttttcatatg gcacagacaa
     1321 tagtgtacat cctccacact ctctaaagtg cttgcaagtt tcttcagttt caagtggtga
     1381 gttctctaat aattctatca tcttaacttc agctttctta cttctaactt ttttaactcc
     1441 agctctcacc ttttgaccac ttattccacc cttcatatgg ataactctat ctccaacttg
     1501 gcttaaagat gtaccaccaa attccatttt atctacttca aattctataa catctttttt
     1561 cttcacagac attcctccaa tttactatag tatctttatt atctttcttc aatcaatttt
     1621 acttcttcat ctgttaactc tctatactca cctaactcta aactttcatc caattttaaa
     1681 ttgcccatag acaatctttt taagtatact acttttttgc ctacgctttc aaacattctt
     1741 tttacttggt gaaacttacc ttcatgtatt gtaagctcta tttctgattc atcatcactt
     1801 tttaatatat ttaactgtga tggcatagtt ttatatccgt catcaagcac tacaccttct
     1861 aaaaaagctt ctacatcttc ttcagtaact actccatcaa tttttgcata ataagtctta
     1921 ggcacatgct tcttaggaga taaaactcta tgagaaagct ttccatcatt agttaaaact
     1981 aataatccct ctgtgtcttt atctaacctt ccaacaggaa atggttcaaa agctaagtat
     2041 gataaatcaa ttaaatctaa tactgttggg tcatatttat ctgtagttgc tgatatatat
     2101 ccatcaggtt tattcatcat caaatatata tattctctat atataacttc ttctccatta
     2161 aataatatct catcattttc agtgtctact tgagttcctg gatttgaaac ttcacttcca
     2221 tttactacta cagacccttg tttacaatac ttctttattt cacttctact tccataacct
     2281 aaatttgaaa gaattttatc tatacgttgc tttttagcca ttaaaaatgc tctccattct
     2341 cataatattt ttctttttca aatactgtaa atgctaaatc tatactttct acatctaaat
     2401 tatttcttat actatcagtt attattcttg ctactatatc ttgtacttct tgtcctctat
     2461 caaaccaagc tacttcaaca aaaggatata catctgctat tttaccatct cttattgcaa
     2521 ctgactttat acattctatt tcaaagtagt ctcttggaca ttttacagct tcaactaaat
     2581 catttatcat tttttcactt attttacata tatcattttc attaattcct cttattttta
     2641 tctgtggcat agtttatctc ctttatttta aatttttagt gaattatcat ccacttaatt
     2701 cctttcgata acgtttgaag tggtaacttc ttggtcaagg caactattgt tatcagtatc
     2761 tccaaactaa taaggtagtt cctaccctta tagttagcat tatatagcta gttttcctaa
     2821 attgtttata ttctttatta ttttatttaa tcctattgtc ttctatttaa cattatactt
     2881 gtaatttata gttattacaa tttactatat actttttttg atttaacgtt ataatataag
     2941 tgatgggtaa attttaaaag ttaataggag gagtttaaaa tggcattaat tactacaaaa
     3001 gaaatgttta aaaaagctta cgaaggtgga tttgctatag gtgcatttaa cataagtgac
     3061 ttagaacaat tacaaggtgt tttaaaagct gctaaagcta aaaattctta tgttatgata
     3121 caagcttcta tgtcagctgt taaatatgct ggacctcata cattagttga aatggttaag
     3181 gctgcttctg atgaaatagg tgtagatgta gctcttcact tagaccatgg tccaaatatg
     3241 gatgctatta aaacttgtat tgatgctggt ttctcttcag ttatgataga tggttctcat
     3301 tttgactttg aagaaaatgt tagaataact aaagaagctg ttgaatatgc acactctaaa
     3361 ggtgttgttg ttgaagcaga acttggtgtt ctagctggaa cagaagatga tgttacttct
     3421 gatgttcaca agtatactca accagctgag gcagttgagt tcgttgaaag aacaggtgtt
     3481 gattcattag caatagctat tggaacttct catggtgctt tcaaattcaa aggagaagct
     3541 aaattaagat tcgatatact agaagaaatc caaagcaaat taccaggttt cccaatagtt
     3601 cttcatggtg catctgctgt tgaccaaaac gctgttgcta catgtaatga atttggtgga
     3661 aatatagctg gtgctaaagg tgtacctgta gatatgttaa gaaaagcatc ttctatggct
     3721 gtttgtaaaa taaacatgga tactgactta agactagcta tgactgctgc tatcagaaaa
     3781 ttcttagctg aaaatccaaa agaatttgac ccaagaaaat acattggtgc tggtagagat
     3841 gctatacaag ctgtagttga aagcaagata gatgatgttt taggttctgc taactctata
     3901 aactaatata acttttaaat atttataaaa gaaaagagat gacttaaagt aattaaattt
     3961 atttttaagc catctctttt aagttgtata aattatgtat cttataatat tttaattata
     4021 ttgtcttttt aaactgacag ctaaacgtga atatgtccac tctttatctt tctatggatg
     4081 gtagaaggat atatacctat aacttcagca gcctttgtta ttgaaccata cttatttata
     4141 gccttactta tcattatttg gtccactatt ttatatgcat cattaagatt cataattcca
     4201 tttaccacta ttggtgataa atcatctgtt tcattatcaa tcatatctaa atttatcagc
     4261 atgttaaatt catcttctcc aattacattt ttagctgata atactacaaa tctttcaatg
     4321 atgtttttta attctcttat gtttcctggc caagaatatt cttctaataa ctcaataact
     4381 ttaggcgaaa cttctacttc tctattgtac ttttcattgt aaagttttaa gaaataattt
     4441 actaatggta ctatatcctc ttttctttct ctaagaggag gtatttttac tggaataaca
     4501 cttaatctat agtacaaatc ttgtctaaat ttaaggttat ttctaaggtc ttcatgagaa
     4561 atattagtag cacttatata tctaacatct atttttatag gcttactacc acctattcta
     4621 gtaatagtat tttcttgtat aaccctaagt aatttttttt gcatctgcat tggtaactca
     4681 cctatctcat ccaagaaaat agttccacca ttcgcctctt caaaaagtcc ctttttacct
     4741 tttttcttag ctccagtaaa agaaccttct tcatatccaa aaaactctga ctctaataat
     4801 tcatttggta tagctccaca gtttatagct ataaaaggtt tgtctctcct tgaactatac
     4861 ttatatattg ttcttgctat tatttctttt cctacacctg attctccatt tataaatata
     4921 gaagaatctg aggctgcagc tctcttaaca acttttttta tattgtccat aatcttactt
     4981 tgacttatat actctgtgtc ttctgatata tcactatatt ttatctgaga ttcaagctta
     5041 ataacccttg aaatatctct ccctgttagt actcctcctg taagctttcc cttagaatcc
     5101 catcttggaa ctgccgtata tgcaataagc ttcccatctg ggtaaacgat atttctataa
     5161 atctttttct tcactttaag catgtttaat attattggtt cttcaactaa tccttgtctc
     5221 acaaggtctg ttatatttct accaagtacc tcagttcttt taaaaggtaa tatctcatca
     5281 ctgacagtgt tcatcttttc aattacccca tctttgttaa acacaacaac aacttcatct
     5341 atatgctcta ttatatcttc aattacttta tcagatatta gagacttctc aaataaataa
     5401 actatattat tttcattatt attatctttc atattgtata tatgtgcagt catccttaac
     5461 tctctgaaag ctattttctt tttactatct gtcagtattt ctgttttgtc aaaatttgaa
     5521 tctaattcat ttatatttct aggctttttt atatctagaa aactaaaaat actatctatt
     5581 agatgattac agtatattat ttcgcctgat tctttacata aaatggctgg attttctaca
     5641 tgttctataa gccctattaa attattatcc attaatattc gcacctttca ttaagtaatt
     5701 tattgaacta catacaaaat ttattgtttg cttacaataa ataccatcag cacatataat
     5761 caactcctta taatattcgt cttttcaagt agtttatttc attaaaacgt aactaagata
     5821 taattttaat gattatagtt tgttaaaaat ttcattttgc ttttaattgt attttatcac
     5881 atatttagta attatttgtc tatttaatac aatttaatta aatttaagat ttatttaaaa
     5941 aaacaaagat aatccttaca gaatataact cacaatttgt aagcaatata catataagga
     6001 ttatccagat tatcttgtat tataaaattt tattcaaaca gactttttaa taactgacaa
     6061 atacacacta gcaataactt atcaaagggt taataattat gttactaact aataagactt
     6121 attcaatcag taatatttat atagtttgta tctattaagc ttttatagct tctaataaat
     6181 catcaacgct atctaatttt tctataagca ttggaactat ttcatgtaag tctccaacta
     6241 ctccatagtc agcgatttct aaaattggag ctgctgggtt tttgtttata gcaattatga
     6301 aatctgatga ttgcatacct gctaaatgtt gtattgctcc tgatatacca caagctatat
     6361 ataggtttgg tttaacagta gttcctgttt gacctacttg gtgagaatgg tctatccatc
     6421 cagcatcaac agccgcacga gaagaaccaa ctactccacc taatttgtca gctaatttct
     6481 taagcatttc aaatccttct ggtccaccta atcctagacc acctgatact ataacatttg
     6541 catctgtaag agatactaaa tctttcttag ttttaactgt ttctaaaaca gtagttctta
     6601 tatcatcttg agttattttg tagtctaatg ctataacttc accagttctt gtttcgtctt
     6661 tttcagcttt atccataaca ccaggtctaa ctgtagacat ttgaggtcta tggtttggac
     6721 aaatgattgt agccattatg tttccaccaa atgctggacg agtttgtttt attttcttat
     6781 cttctgggtc tatttctaac ttagtacagt cagctgttaa tccagttcca actcttgaag
     6841 ctattctagg tgctaagtct ctacctatat gagttgctcc gaaaagcatt atttctggtt
     6901 ttatttcatc tattgcatct tttattactt ttgtatatgc atcagttgta tatttttcta
     6961 aaagagcatc atctgcaaca taaactttgt cagctccaaa agtgattaat tctttagcta
     7021 atccatcaac atcttttcct aataatattg cacaaagttc tgcatctact tctttagcta
     7081 attttcttcc ttctcctaat aattctataa ctactggagc tatttttcct tctctttgtt
     7141 ctgcaaatat ccatacgttt ttataagaac ttaaatcttt tatatcattc atagtttgta
     7201 ctcctccaac ttatataatg tgcttttgtt ttagaccgat tattatttta ctagctattt
     7261 catcagcttt accttctaag atttctcctt ttcctcttgg ttcaggagta aatgatctaa
     7321 atactttagt tggtgatgct tttaaaccaa cttcttcttt atttacatct aaatcatcaa
     7381 ttgtccaaac ttttacatca tgtttatatg cttttactat tttatcaaca gacatgtatc
     7441 taggtgtatt taactctttt actgctgtta aaagtacagg tgtacttact tttattaatt
     7501 cgtatccatc ttctaattgt ctttgaacta ttatatcttt tccttctatt ttgaaatctt
     7561 gtacatatgt aacttgagga atgtctaatt tctctgcaat ttgtggtcca acttgtgcag
     7621 tatctccatc tattgcttgt cttcctgcaa atattatgtc atagtcacct actttagata
     7681 ttcccgctgc aatagtattt gatgtagccc aagtatcagc tcctccaaat gctctatcac
     7741 ttaataatat tgcatcatca gcacccatag ctaaacactc tcttagcata aagtctgctt
     7801 gaggaggtcc cattgttata actgttatag ttacatcatc atgctcatct ttaattttta
     7861 aagcttcctc taatgcattt gcatcatctg ggtttaatat acttggaact ccatctctta
     7921 taagagtacc tgtttcttta tttattctta cttcgttagt atctggaact tgttttacgc
     7981 aaactaatat tttcaaagtc tatacctcca atttcattag attatttatt attttaatac
     8041 gtttgatgaa attaccattt tttgaacttc tgatgttcct tcgtatattg atacgattct
     8101 agcatctctg tacattcttt cgatttcata atcttttata aatccatatc caccgtgaag
     8161 ttgtaatgct ttataagcaa cttcatttgc aatttctgat gcatagtatt tagccatagc
     8221 agattcttga gcaactctca ttcctgcatc tctcttttgt gctgcatcat aaactaatcc
     8281 tctagctgca caaacttttg tttccatgtc agctatagta aattgagtat tttggaattt
     8341 agctataggc ttaccaaatt gaactctttg ttttgtatat ttaacagctt catctaaagc
     8401 accttgagct attcctagag ctaaggcagc aacacctatt ctaccaactt ctagagtacc
     8461 cattgctatc ttaaatcctt gaccttcttt tccaagtaag ttttcttttg gaactttaac
     8521 gttttcaaat attaagtcag aagtttcagt tcctcttata cccatcttat cttcatgagc
     8581 tcctgttgag aaaccatccc atttactttc tactataaac gctgatgttc ctctaagtcc
     8641 tttacttcta tctgtaactg ctattactat agctacatca cagaatggtc cattagttat
     8701 gaaagttttt ctaccattta atatatagtg gtctccaact aattctgctg ttgtttgttg
     8761 tccacctgca tcagaaccag ctcctggctc agttaaacca aatgctccta ttaattcacc
     8821 tgttgctata ccttttaagt atttttgttt ttgctcttca gtaccattgt aaagtattgg
     8881 taatgaagca agagaagaac cagctgtaac aaaagtagct gttgaagcac atcttctagc
     8941 tatttcttcc attactatag cgtaagaaac atatcctgct cccccaccac catattcttc
     9001 tgggatatta ctgcttataa attttgcatc agctaatttt ttgattaatt ccttaggcat
     9061 tacacctgtt ttatccattt ctgctggtaa agtatctaat tctttactaa caaaatctct
     9121 tactgctttt cttaaaagtt cttgttcttt gttatataac atagcttcct ccaattttca
     9181 tattttatag taaatttatt ttaatgaact tacgctaaac tcatcatctc agcaaaagtt
     9241 tgaatacgag ttctggcttg ttcattattc tgagtttgtt ggtcgatttc aacatataaa
     9301 gtaggtattc cactatcttc tatatctttt ctaactaaag gataatcgta ttcttctggg
     9361 tcacagaatt tcatcataca gaagataaca ccatctatat ctttcttttt aacttcatct
     9421 actataagtg acccacgttt tttcttaggg tcataagcta atgaacatcc ttctatgttt
     9481 gaccattgtc ttgctaatct ttctaacgca tcatcacctg ctggtacatc tgttctgaat
     9541 tgtcttgttt cttgtgctaa gtcgtcagct acaactgata tattgttgtc ttctaaaatg
     9601 tctaatatat ctttagaatc agctaatata cctgttaata aaactttctt tccagaacag
     9661 acttcttctg gcatagcatt taattttgct attaaatctt tcactagctc agtatgttct
     9721 tcttttctca taaagaaccc actcttaatt actaagcttc ttattgatgg ttttatagta
     9781 ttagaatgtt tataagctac ttcaacaaag tctctcatag tttttctatg ttcattgtaa
     9841 acttctatac tttcatgaat ttttgcttct tctatttcat atccacaaat ttcttcaagt
     9901 tctcttttta cgcctttgta ctcactgatt aagtatttta caccagcttc aagttttcta
     9961 ttttgtgggt gtactaatga tatatatttt atatgaggta ctgctgattt ccagttttgt
    10021 cctaaacaaa ttagtgtatc acacatacct ggtataataa ctccagataa ttcatcataa
    10081 gcacctttta atccatattc taaacatgat tgcattattg aacatgcaaa tgcagggaaa
    10141 tattgtttag ctaaatctaa ttctgtttgc cctccccata taccaactgg aagcattcca
    10201 gctgcatgta taatttcttc tgggcaataa actgggaaac aacctatagc ttttttacca
    10261 gtttcacttt tatatttttt tacagccgca tttggatttt caactacttc tttcatttta
    10321 gataaaatag cttccattat atctcacctc tattaagttt ttttctttct tccattactt
    10381 caactaaacc ttgaattctt gtttcaaatt gagcattagt gaaagctcta gggtctgctt
    10441 ggtcaccatc aaatccagca tatggtaatc cagtttcttc agctgctctt ctttgcattt
    10501 catattgtat taaactcata agtttacagc ttctgttcat atgatagaat gctccatcac
    10561 atttaccctc tactaaagaa tcaactctat attttgtcat acggtctagg tttacattgt
    10621 taaacatagt actatatgct actgccattc catctaaatc attaacttca tattgtaatg
    10681 cccaagcatg tgggtaaaca ctacctgtca tgttaactcc aaatttagct aatgttttca
    10741 tcttgtatcc tatatatggc caacaaggta taccttccat cattattctg tatttttctt
    10801 cccctctgaa agaagattta ccagttttca tgttgtcctc taattcttct ataagtaact
    10861 taaatgcttc tgtagtttct tttttacctc tagcacaaac tattacagcc atgtaagtaa
    10921 ataagtcaaa tccattcatt ggagaaggag aagaatctgc tggtaaactc atagaatact
    10981 tccatagtct tccgttttca gctgatattt tcattacttc ttcaaacttc ttagggtcaa
    11041 atttctttcc tgatataatt tctagttgtt ttatagcttc ttcaaattga gctttaatat
    11101 aatctattct tgattgagta acttcgtctt cattattgaa agttgtatca atcattatta
    11161 aaggtatatc taattctctt gaaatatttt cataccattt tataacttgg ttacatatat
    11221 tgttacagca aagtaggaaa tctggagctg gcatatccaa agcttcacat ccaccatttt
    11281 ctaaaagacc aaaatttgtt cttgcatatg cacataggtc aatagaatat cctttagatt
    11341 cagctatttc acataattct aaagaacctt ttttagctgc tactccagct gcttggtttt
    11401 ctggatataa tacgtttaag tcaaatactt ctgctaactc ttgaggaaat actgatgttg
    11461 accaacctac aggtcttcct tcttctttag ctttaaatgc atttgcatat tgttcagcta
    11521 ataaatcatt aattactact ctagcttctt ttttttcaga cattttcatc cctcttttta
    11581 tatatttttc acttcttttt gtgattcttt tgcttcatca aaagcatata acgctgctcc
    11641 taaagcacca gttaattgag gtatatcagg tacaataatt tctgtgttga tttctcttgc
    11701 catagctctt acaataccac tatttctagc aactccacca accataacta catttctttg
    11761 tactcctatt ctttttacta ggctagaaac tctctttgct actgaagtat gaatacctgc
    11821 cactatatct tcaatttttg cattttcaga taaatgtgat ataacctcag actctgcaaa
    11881 tactgtacat gtactgctta ttgatacttc attttgagaa ttcatagata tacttccgag
    11941 ttcagataca tcaacctcta ttatttttgc cattacatct aaaaatcttc ctgtacctgc
    12001 agcacatttg tcattcataa gaaagtttaa tagtcttcca ttattatcta attttaatac
    12061 ctttgcatct tgaccaccta tgtcaataat ggttcttgtc tctggaatta tgaaatttac
    12121 ccctctagca tgacagctta attcacttat ttgcttatca gcatctgaat agttcattct
    12181 tccatatcct gtaactacaa cttttttaat atcttctctt gcaagacctg tcttgccata
    12241 taatttttct aaaactcttg atggtccagt agtcccagta ccagaggata ttgtttcaga
    12301 agctacaata tcttccccat tctttaagat tactcccttt gatgcagttg aacctatatc
    12361 taatcccatt gtgtacatct ttagcattct ccttttaaaa aatctagaat tactaattaa
    12421 tatcttacaa ctttactatc tttaaagtta tttattttct cttcatcgta gcctaaagat
    12481 tttaatactt ctacagtatg ttgacctact tttggtgctg gtgtatattc tttaattcct
    12541 tcatttctaa acataactgg agtattaact aagacacctg tatttccgct atcgtatgtt
    12601 ttcttaaata agaagtcatt tgcccaagct tgttcgtcat ctaataaatc ttcacagctt
    12661 tgaatttttt caaatggtaa gtctgcttct tctaataaag ctgaccactc gtctaatgtt
    12721 ttttctaaca tagcttctcc aactatctta actaaatctt caacatgatt aaccattgaa
    12781 tctatgttat tatatctatc atcttctaat atatattctc tatttataac cttacagaat
    12841 ttgcctaacc acttgttgta ttgtattaaa gctaattgaa tccatcttcc atctttacat
    12901 ttatatgtag tcattaatgg gctgtttgga ttttctcttg ataaaggcat ttcatttccg
    12961 tattgtgctg ttgttatcat tgttcccatt ccatatatag ctgtatggaa aagacttact
    13021 gttactctct cacctttacc agtttgagct tttttatgta atgctgctaa acttcctgct
    13081 gctagtgcta gacctgcata gtggtcacca aatcctgctg ctgtatttgc tggagatgtt
    13141 cctttttcca taacagattg gctaactcct cctcttgcga agtatgcagt atagtcaaat
    13201 cctggtttat cttttaaagg tcctttttca ccatatccta atatttgaga gaatattaat
    13261 cctggatact tatcttttat ttggtcataa gctataccca ttttttctaa tgcttgaact
    13321 ctaacattag ttacaaatat gtctgcttct gataataatt tatgtaatat ttctactcct
    13381 tcttttgatt ttacattaat acttacaccc tttttatttc cattttctaa ttcaaacata
    13441 gggttttcat catctgatgc tggagattta aatgttccac ccataactct tattccatca
    13501 ccttctatag gttcaatctt aataacctct gcaccccagt cacctaacat ttttgcacaa
    13561 catggtgctg cgatgaaact tgaaagttct actactttaa ctccttctaa aagcataacg
    13621 ttttcctcct taatagtatc aattataaaa tttcgattaa atcactcata aaatactttt
    13681 ttctgatatt aactgtctga tgtcaaagtt cctaatctta tctaaaataa cttaaaaatt
    13741 attaaaatta ttttttattg ttttattgtc ttacacctat attgttaagc atttatcgtg
    13801 ccaactttat atttaatcaa aatatcgctt gatttgaatg ttttttaaca atatgcaatt
    13861 gaataatctt tttcatatcg tggcatcttg ccactatcca ttgcattttg ctattctttc
    13921 tatgcttata aaagtttttt ttatatattt ttttatgaaa aagcttgaca tttcaaaaaa
    13981 agttttcatt gctaaactca aaaacacatg ttatcattgt aattaatcag caagttagtt
    14041 aactaatttc aaacttagat aattttttaa taatatttaa tctaaaaaat aacaatttct
    14101 ttgataaatt ttagagtaat tattacatta aataaaaaat tactaaatat caaaataaca
    14161 cagggaaaac aatttcaaaa ctaaattttt aaccttaaaa attttgtata aaaaatataa
    14221 ccttatatgc tacatattat ttctaaatga aaaattacca taataattta ataattacag
    14281 gagggtattg atatgaaaat actagtattt ggagcacgcg attatgaaga accagtaata
    14341 aaaaaatggt ctgaagaaca taaggatgtt caagtggata tttatcctga aaacatgact
    14401 gaagaaaatg tagttaaagc taaagggtat gatggtatat ctatacaaca aactaactat
    14461 atagataatc cttatattta tgaaacttta aaagatgctg gggttaaagt tatagcttca
    14521 agaactgcag gggttgacat gatacatttt gatttagtta atgaaaatgg acttatcgtt
    14581 acaaacgttc cttcttattc acctaatgca atagctgaat tagctgttac tcaagctatg
    14641 aaccttttaa gaaagactcc tctagtaaag aaaaaagtct gtgaaggtga ttaccgttgg
    14701 atagctgaac ttcttggaac agaagttaga tctattacag ttggtgttat aggtacagga
    14761 aaaataggtg ctacttctgc aaaattattc aaaggcctag gagctaatgt aattgcattt
    14821 gaccaatatc caaatagtga tttaaatgat atattaactt acaaagattc tttagaagac
    14881 cttctaaaag aagctgacct tataacatta catactcctt tacttgaagg aacaaaacat
    14941 atgataaata aagatactct agctataatg aaggatggag cttacatagt aaatactggc
    15001 cgtggtggtt taattaatac aggggattta atagaagcac tagagtcagg aaaaattaga
    15061 gctgctgccc ttgatacatt tgaaactgaa ggattgttct taaacaaaaa aatgaatcct
    15121 ggagaattaa ctgacccaga aataaataaa cttctttcta tggaacaagt tatattcact
    15181 catcaccttg gtttcttcac tagtacagcg attgaaaata tagtttattc tagtttaagt
    15241 agtgctgtag aagttataaa aacaggaact gctactaata gagtaaatta gtattttata
    15301 aatatatttt ttcattaatt agctattatc taacaaaata gcctttaatt tcccaaatat
    15361 aagagagtca gctcagtata tattgagtag actctcttat attatttttt atattaaact
    15421 tgtactcttc ttattcatac caaattaaat ttatattaac ttattctaac tactcctact
    15481 ctatacattt tacctatttt tacttttcta attatataca tttgaatggg tacaatctgt
    15541 attttacaaa aaaaacacct aacaaactat gaaaattgta tgattaatat taatatattt
    15601 ataagaaaac ataacaatta tgttagctct ggatttttag ggagggagta tattggaaaa
    15661 aatgaataaa gttacttcta aaaaaactac ctatattgtt acttcagcat tatttgcatc
    15721 cataatatgc ctgacaatag catatatatt acatattcca gttggtggaa acaatggata
    15781 tgttcatata ggagatgctt ttatttatct agctgcaact attttgccaa caaactatgc
    15841 tatagctgct tctgcaattg gtgctggtct agctgattta tctacaggtg ctgctatctg
    15901 ggtaatacca actataatca taaaaccaat acttgtatta tttttcactt ctaaaagtga
    15961 caagataata aataagagaa atatagttgc ttcggttgta gcaggtatag taggattagt
    16021 tttatacatg tttgctgaag gtataattat tggaagtttt acaagtgcat ttgtaatgag
    16081 cttacttggc ttattacagc caataggaag ttttattgtt tttataatct taggtatggc
    16141 acttgataaa cttgatttta aaaaacgtta ttttaattaa gaatttaaat ttatggaggg
    16201 aaaattatga atatcttata tacaattgaa aatagtatct atgtaaatat aactaataca
    16261 tgtccatgta gttgtgtttt ttgtataaga aacgaaaaag atgaagttgc aaatagtggt
    16321 agtttatggt tggagcatga gccaagtgta gatgaagtaa aagaagcctt caataaatac
    16381 aatttagacg actatgatga aatcgtattt tgtggatatg gcgaaccatt aatgagaata
    16441 aatgaattga tagagatagc aaagtttata aaagaaaaaa gtagcataaa gataagaata
    16501 aatacaaatg gtctaagtga tttaatacat aacaaaaaaa cagctattct gttaaaaaat
    16561 gttatagatg cagtatcaat aagtcttaat gccccaaata aagaagctta taatagagtt
    16621 actcaaccaa agtttggaga aaaatcattt gattatatgt tagattttgc aagagattgc
    16681 aaaaaatata taaaagaagt agctttttca gtagtagatg aaatatctcc agaagaaata
    16741 gaagaatcta aacaactggc aaaaaaactt gatataccat taagagttag acataagaat
    16801 tagcaagtaa aattataaaa cattaagaat cacctgaatg gtatttcagg tgattcttcc
    16861 tcttgtatta acattttaca aaatagattt cataccacac taggaatgtg taaatcatcc
    16921 aaatctttct ccaattatca gagtttccac taatgtagtc attaaaaatc tcattaatct
    16981 catctatgtt aaagaattta tctgctatct cactattgaa tttatctcgg atatccttat
    17041 tatatcggtc atcagccatc catatacgaa ttggcacaat aaatcctaat ttatttctaa
    17101 aagcaatctc ttctggtaat gtttttgatg cagctgtacg aaaggcaatt ttattctctt
    17161 ttcctttcac tttatattct gatggtattc tggatgcaat atcaaatatt cttgtatctg
    17221 taagaggcat tctgacttcc aatcctactg cagtactcat tttatctata ttaaaataaa
    17281 tatctccttc caaccatatt tgaatatcta catttgacat gttagtaagt gtatctagtt
    17341 cttctgtttc ttcataaatt ccttttacca cttcaatagg aagcacatca ggattgtatt
    17401 tcttaagaat ttttcttttc tcaccctcat tcataatgtt tgtatttcca atataaaatg
    17461 tcttcaaatt ctctccataa cactctgcct tatggtatat attatatcca ccaaaaaact
    17521 catctgctcc ttctccagag taacagatat ttgtgttttc agctgctgcc ttgcatccaa
    17581 tagcaaaaac gatagcagat gcatctccag ttggttgctc catatggtac attacataag
    17641 gtactgacgc aaaatactct tcaggtgtaa ttatatatct cgaattttta cacccaagaa
    17701 tattagctgt ctgttgtgct aattttgatt catcaaaacg ctcttcctca taaccacatg
    17761 aacctgtttt ttctatatct gacattgcca atatataaga tgaatcaaca cctcctgata
    17821 ggaataactc tgcagtttca ttctctgatt ttacctctgg cataattttc tgaagtgttg
    17881 tatgaatttc atccgtccat tcctcaatag acttgctgtg gtcaggatga aattcaggtt
    17941 tccaataacg accaatacat actgttttat tctgatagac aaggtaatgt cctggcataa
    18001 gcttctttac tccacggaag aatgtatttt cacctgctac atatgtgaaa gacatataaa
    18061 tctgtaacat gtcttcattt aactctttca caaatcctgg ttgctccata atcttacgaa
    18121 tagatgaacc atagagaagc ttcccatttt cagtctcata ataatagaaa ggttttaccc
    18181 caaactggtc tcttaggcag aagagtttct tttccacagt atcccataat gcaaaagcaa
    18241 acattccatg catgtgattt gccatatcat atccccatgt cttatatgct tccactaaaa
    18301 ttgctcgttc acgctcatct ctacataatg aagaatcgat tccaagttct tcacaaagaa
    18361 ctttccagtt tcgaatatgc ccattgtatt ctagaatttt tatcatatat gtgtcttttc
    18421 gtgttaaata cttttcttct aaatttatca tatgtgtatc ctttcgtatt aaaacattta
    18481 acttctaaat ttattaaatg tttatatata tttattcact tataatcact gaaaaatcga
    18541 cttttttcaa agtaaattct ttttgaatta gtactaatct gctctttatt aatggcataa
    18601 atatgattat caagtattat cagtgttata acattgaatt ttttattctt cataaa
//
LOCUS       DAELTA010000075        18633 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.075, whole genome shotgun sequence.
ACCESSION   DAELTA010000075 DAELTA010000000
VERSION     DAELTA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.075"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            259..1437
                     /locus_tag="KPG13_002136"
     CDS             259..1437
                     /locus_tag="KPG13_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0985852.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1787..2668
                     /locus_tag="KPG13_002137"
     CDS             1787..2668
                     /locus_tag="KPG13_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG0985853.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2653..3026
                     /locus_tag="KPG13_002138"
                     /pseudo
     CDS             2653..3026
                     /locus_tag="KPG13_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3286..5853)
                     /locus_tag="KPG13_002139"
     CDS             complement(3286..5853)
                     /locus_tag="KPG13_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG0985854.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5856..6461)
                     /locus_tag="KPG13_002140"
     CDS             complement(5856..6461)
                     /locus_tag="KPG13_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985855.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(6835..8295)
                     /locus_tag="KPG13_002141"
     CDS             complement(6835..8295)
                     /locus_tag="KPG13_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG0985856.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8657..9235
                     /locus_tag="KPG13_002142"
     CDS             8657..9235
                     /locus_tag="KPG13_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0985857.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9526..10776
                     /locus_tag="KPG13_002143"
     CDS             9526..10776
                     /locus_tag="KPG13_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0985858.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10937..11506
                     /locus_tag="KPG13_002144"
     CDS             10937..11506
                     /locus_tag="KPG13_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG0985859.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(12084..13424)
                     /locus_tag="KPG13_002145"
     CDS             complement(12084..13424)
                     /locus_tag="KPG13_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0985860.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
     gene            complement(14094..15185)
                     /locus_tag="KPG13_002146"
     CDS             complement(14094..15185)
                     /locus_tag="KPG13_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG0985861.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            complement(15953..17872)
                     /gene="thrS"
                     /locus_tag="KPG13_002147"
     CDS             complement(15953..17872)
                     /gene="thrS"
                     /locus_tag="KPG13_002147"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG0985862.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     misc_binding    complement(17936..18174)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         6439 a         3100 c         2251 g         6843 t
ORIGIN      
        1 tatgtaatta ctatacttat acacataact attatacttt ccatacataa ttactatatt
       61 tggacacata gttatcatac atactgtata ttaagatata atacttttta aattttatat
      121 aacacagaca catgcaataa aaaagaaggt atccataata agctctttgc atgtacctaa
      181 ataaaaactg taaaatagaa tatataaaat ctaatctatt ttaagtaaat acaatttcac
      241 tttaaaagag gatgtactat gaaataccat atatttttaa tcataactac tatattaata
      301 atcgaactat gtaacattta catatgcaaa atagcatctt ttggaaacaa aaatataact
      361 atacataaga attacaaata tattttttta ataaaaccat taaccttctt gataataata
      421 ttcacaaaat taccattacc actatattat atattgtttt acatagtaag tcataaattg
      481 ctcttaagca gaaaaagttc tactaacaac cattttattc ccaatatggc agtaataagt
      541 ttttgtattt tacaccttat tactgtaagt acaatatcac ttgttaaaaa tacaagtcca
      601 gatataatat ttgcaaatat gacctataag ttaattagta ttttaatagc ttctatttta
      661 gattttatct tatggtactc tatgaataaa ataaaagagc atatttatat aataactcaa
      721 aatcaaatag agttcagtct atttttaaaa tttatctggt tcactttagg atatatatta
      781 tttgatagta tacaaattac atttgctcca ccaagttctc taacttccta ctttatattg
      841 ggaagtaaca tattattaat cttacaacta attatattca tcaatcatat atattccatt
      901 gaaaaaaata tttatttaga aaaagaacat aatagactaa aaatgataaa acaagaacaa
      961 ttactaaaag taaatttatt aaagaaaaaa gctaacattg atgaattaac atcagcatat
     1021 aatcgaaaat ttgcttttga aaaaatacag cattacattg ataacaattt attattttca
     1081 ttgatatata tcgatttgga taaattaaag acgataaatg atacatatgg acatttagct
     1141 ggggataact atattattaa cttctcaagt atagttattg aaaacttagg aatagatgac
     1201 atatttgcta gaataggagg agatgagttt atagttatta tgcctaaatc taatgaaaac
     1261 accacaaaag aatttataga gaaaataagt aaaattatgg ataatacgat atcttttagc
     1321 tatggtattg tagaagtttc tagcgaaaac aaaatgactg tagaagaaat tattaatatt
     1381 gctgatatta gaatgtacac taataaacaa aataatcata aaaggaacaa taactaaaat
     1441 gaatatttac atgagcttac ttgtagtctc actatgtttt ttatcttatt atctctatca
     1501 ttataatttt tttaaatatc tggacataat gattaataac tctaaaaata aatacacagt
     1561 atatattata tattctatat ttaattattt aatttttatt atttgtactt atttaaagtt
     1621 tcatctaatt ataaattgga caatttttat gttgatttta ttcatacaaa ttaaagtaac
     1681 ttataaaatc tccacaataa aaaacatttt ttattcacta aactgctcca tcattggttt
     1741 agcaataaat atatttttta ggtctttaat gtcaatagtg ttaaatatgc cagcatattt
     1801 atttgatagt aattctattg caaatattta cagagtattt cctatatcat ttagctttat
     1861 agttacagga ctatatttca gttataaaaa taataaaaat aataagatta actttgaggc
     1921 agttttagag gacaaaaaaa acttaatttt cttattgagc cttatgtttg gaatgtactt
     1981 gtatctatgt ttgagtttgt caatatacta tactgttgga aatgtattga ttttaaaatt
     2041 atatggaatc aagtctgcca taatagtttt ggtaggtaac tatattgcag aaagatatag
     2101 ctttagaatt actcaaataa aaggttatga tattaaaaat caaatagaat atgtagaaag
     2161 tgtaaagaca gaatatgaga ttaataaatt gaatgatatt gtatatacag atttactgac
     2221 aggtttctac aatagacctt ttgtgaatca aaaactatca gaatggacat caaatcacta
     2281 caaatttacc ttatgtttta ctgatttaaa tagcttaaaa cctgtaaatg ataaatttgg
     2341 acaccaattt ggtgataaat acatatccac tactgctcaa ataataaaaa aacttataaa
     2401 tgtagaaaat tccttactat ttagatatgg tggagatgaa tttttattcc ttttacagaa
     2461 tacaagttgt gtagaagcag aaaagataat gattaatata aattatgaat tatcaaaatt
     2521 aagtaatact gacgagtatc cttatgctct ttccataagc tatggattag ttgagtggga
     2581 tggaatatcc gatattgaaa ctttaatatc aaaggcagat tctttgatgt atgaaaataa
     2641 gcttaaaaat aaatctactt taaactagct agttaagata ttctacaaaa tatatgttat
     2701 aagagagtac taagaatatc aaaatatata gattttgcaa atattgaata cagtttacta
     2761 atgaatgcac tggatgtaag tgttagtaaa cacctattag atgaacactt tactgttgtt
     2821 tgggctaatg accgttatta tgaaatgttc agctatacct agctaaaaag ttaaaaatga
     2881 ccactgtagc cgagggaata gagaccagtt ttcaaaaaga tttcttgcaa gaaataaact
     2941 gtgatatgtt acaaggatac gtgttctcta gacctttacc aataaaagac tttgaaaaac
     3001 ttatgtttca aaactcctcc aattaacaat ataaaattgc ttattataag tgatattatt
     3061 gtatcaacaa ataaaggaat tgtctcaaaa ttgatttttt ctaatcaaat ttttattttg
     3121 agacattctc atttttttgc ataagaaaat acccaccaag ctacggtttt tcttaccgct
     3181 gttcggtggg tattagtagt attatgaaat ctgaaaactt taagtattac tacttccgct
     3241 aacgcaatca caaaggtcga aagcttcaaa gcgaaaatag gctttttact catcaaacgt
     3301 caccgtacct gccaacccgt ccaccgttat catttgtccg ctcttaatag ttcgtgtggc
     3361 aatgtgtgta gccataacgg ctgggatgcc atattcacgt gcgacaatgc ttgaatggct
     3421 aagaggacca ccaatgtccg ttacaatcgc acttgcagat gaaaataatg gtgtccatgc
     3481 tggagttgtg gtaactgcaa ccaaaacact tccaggttga aagctttcaa aatctgctgg
     3541 actattcagt acacaagctg gagccgtcac aacaccagta ctggtaccaa tgcccgtcaa
     3601 cacaattttc ccatctttct gcttctgttg tgcttgtgat atagtttttt tgtttttctc
     3661 tggcaacttg gatggtgaca tataacccat gtatttttta agttcagctt ttcgaacagg
     3721 tattatcccg ttcatatcag atagagactt gtttttatcc aactgctcta taagtacctc
     3781 caattccgat tttgtgagcc aataaatatc atctaaatgt gaaatagctc ctcctcgtat
     3841 gaatcgttct gagatttcct gaaacataca acgaatcaga ggatgtccca ttcccatcaa
     3901 ataaatagca ttttcacgca taggagaggt ttcctgtgcc caatggagca acttaaaaaa
     3961 tagtttttta cgcaatccac ctatgtgttg taaaatctct tcttctgcat gttttctacg
     4021 cttttcaaac gtggtctggc gtaaaaaagg actttcccct tttccttcca caaatgtttt
     4081 tacggattca aaagttggtg taagtgtttc ctgcggtgtg gaatgcgcaa aatcaaactc
     4141 ataagcggta cgaccaaact ctttaaagta cttattgatt cgatttttcc attctatcca
     4201 tacttcctgt gaaactccaa caggcacaac tgaagacatg aaatcttctg ctatctttat
     4261 tgcaggactg ttttgcagat aaaggttgat gctgttattt tgctttaccc attctgaaat
     4321 accccacaac tccttttccg attgcagaac aatcgtatca aacccaagca ggaaaacgga
     4381 agtatccgtt ataccagcac gtcgaactgc tccttgaaat aattttgtaa ataatgtttc
     4441 actgatagaa gcagcaggca atgtaagctg aatccttgtg aaataaatac atgcggcata
     4501 aaatattgtc tgaataccct ccataatttg atgggcgttc atagtatgca ggggcttttc
     4561 ttcccattgt ttaatagcat cctcatatcc ttttcgtgcc gtttcccagc gtgcaacgct
     4621 gttcataagt gtcctacgta aagagcgtgg ggaaagggat ttgatagcaa tcaaaagtgg
     4681 ctttgattta gcagaaagat aaacatatcc attaataacc gtatatgccc cgttttttgg
     4741 taataatttt ttcgcacttt taccaaacat atctatccat aaaagctctg atgcacggtt
     4801 tacgatttca agaccaagtg tggcgaacaa aggtgtaaca ggatttggca aatgttctgc
     4861 caaacttccc tttgtgtata caacatcctg ttctggcaat acccattctg gtggcagaac
     4921 agtaattgga cgagcctgaa caatgtacaa tttatctttt tccagtgccc actctacatc
     4981 cataggcatt tgataatact tctcaatttt ctttccaagc tgtatcagtt gcattacctg
     5041 attacgagta agagcatatt tttttctcaa cctttcagga accatggttt cttctgttcc
     5101 ttctgaagta cgaaccgtca taatctcttt atttgccact tcatacgata taattcgttc
     5161 ggagtcctta tccactacga ttgtatcggg tgtaactaag ctagaaacca ccgcttcacc
     5221 aagcccccat gcagcgttaa taatcatttc gctacgtcta ccattgattg ggtttaacgt
     5281 aaacataata ccagatgaat cggaaaatgc aagcttttgt actacaacag caagtgcgac
     5341 aatctcgtgc tttatattat tcttcacacg ataagcaata gcacgagctg tccataagga
     5401 agcccagcat ctctttacag agtcaataac tttatcttca ccttgtatgt taagataagt
     5461 ttcctgctga cctgcaaaag aagcgtcagg taaatcttca gcagtcgcag acgaacggac
     5521 agccaccgat atgttcccta atccagcata agccatttta attgcgtccg acacttcctg
     5581 tggcatttct ccattatgga aaagctcccc tattttctta gatacatctt caagttggct
     5641 ggtattattg gaatcaatac catctagcag tttattaatt cgagactgaa tatggttcgt
     5701 ctcaacaaaa atctggtatg aagcagtcgt aacatgaaaa ccgtctggca cagggatgcc
     5761 tgctgtcaat agttttgata aagacatccc ttttccacca acaatttcaa tggttgcttg
     5821 tttatgttcc agtggcaaga cataataaat cataatcatt cacctccatc attcataatt
     5881 ccgttcagaa atatattcat tgtttcatcc aacatttttt ccgaactatc caatggtcgt
     5941 gacgtgtagc tgattgataa catggagtta attaacaaac gtgtcacaag agcggtatcc
     6001 gttttgcgaa agatgcctgt ggtcacacca ttatcaatta ccgattttat aatatcttga
     6061 tatgcataac gagcatcttt aagtctcttt cgatactctt cttctaaata atcagattct
     6121 gtatttaacc acatcagtac agttctatac tgctctggaa atctaagatg attaagcatg
     6181 atttttctaa ggcactgttg tggtgaaggt tcaccatcaa taatcctatg aacttgttgg
     6241 atagtttttt ctatttcttt ctcaactgca tatacaacaa tctcgtcttt cgttttaaaa
     6301 aaatcgtata gacttgactt tcccatgttg gctaaaactg caatttctct cattgatgtt
     6361 ttttggaatc cattttcagc catgagactc aaagcaacag caactatttt ttctcggcgt
     6421 tctgcttgtt gttcgggtgt aagcacaatt ccttttggca tagtgcgata cctcctttta
     6481 gcgaccactg gtcgtttctt ttttatttat catacagcaa ccaacggtca cttgtcaagg
     6541 ttaatttttt tcattgtata tggtactcga gataaaaatg ttcattttag aaattactct
     6601 atttaagtag aaataggctt catcattaga aagcaagact ataaatttga gatatttatc
     6661 atgttcaaat ttcatcaata tcaaagttta aaatattaat aaaaaaagaa ttactaagaa
     6721 ataacatata aagattcaaa aaaggagttg aacttaaaat tcaattacaa aaaatcaaat
     6781 ttaagacaac tcctttattc tttatctcta ctattcttta tctctattta tttattacaa
     6841 ctccctaaca tttaccacta ctgccttagg tctagtcata gcctcaattg aatacttaac
     6901 accttgagtt cccataccag atgcctttac acctaaaaat gggaagtggt ctggacctct
     6961 ttcagtctta ttatttattt gaacagtacc aacttcaagt ttatcagcta tataaaatgc
     7021 cttatcaata tcacttgtaa atactgatga ttgaagccca tactctgatt gattagcaat
     7081 ttcaattgct tgatttatat ctttaactct tataattgga agtactggac caaatggttc
     7141 ttcccaagca attttcatat caacatttac attatctaac aatgttggat agaataaatt
     7201 attttttcta acattgcctg ttattaactt tgctccttta tgtaatgcat catcaaccaa
     7261 cccttgaaca aaatcagttg ctttattatc tataagagga gtgataacta cttcatccat
     7321 tggatttcca attgtcaact tctctaccaa tggctttatt tttccgacta atttatccgc
     7381 cacactctct tgtactaaaa ttctctttac tgccgtacat ctttgtcctg aatatgaata
     7441 cgctccagat actatattct tagctgcaaa atctaagtct gcatcttcta atactatagc
     7501 cgcatctttt ccaccaagct ccaataataa tggtaccatt ccagatattt tagatatatg
     7561 ctgaccaact tcagtactac cagtaaagtt aataaaatta attcctttat gcgttacaac
     7621 ataatcccct atctcactac ccctacctgt aatagtatta agtactccat ctggtatccc
     7681 agcttttctc ataatttcta ccacatgaag tgcgctaata gccccttgtg tagcaggttt
     7741 taatacgact gcatttccac caataagtgc tggagcaatc tttgacattg ataaatttac
     7801 aggataatta aaaggagata tagctaaaac agtacctaat ggaacccttg aaacataaga
     7861 catcttattt cttgttccac cagggaaatt atctccacta atggcttctc cctccatatt
     7921 ttttcccaca tcagctgtgt atcttaaaaa atctgctgtt ctctcaactt ctgcccttgc
     7981 agattttatg tctttggcta tttcaagtac cagtatatta gctatctcat ctatattttc
     8041 taacaataaa tcagcagcct tatgaaatat attgcctctc ttgtatacag gcatttcagc
     8101 ccatactttt atactctctt ttgtgttttt tataacctca tctacctcat gttttgtcat
     8161 agattgtact ttcccaacaa gagaattatc tattggtgag taaatttcta ttggctttct
     8221 cgaattagat tctacccatt ttccatttat taaattttta tatacatttt cttttgtttt
     8281 aagttcatta aacatatttt tattcctctc tctgatataa ctttgtgtat ttaaattata
     8341 gccatttatt cgttatgtaa acatttcttt ttttcataaa cgtttaccta taaaaaaatt
     8401 ttaagttaac gtttttctac attcttctat tttaataaca tactcgctcc aaatctatat
     8461 aggtcacact ttaatactaa tatatcaata taccaatata ccaatatacc aatataccaa
     8521 tataccaata taccaatata aatattataa acataaatca cttgacaaat ttataagtag
     8581 acaatattat atatctatga ctcaaaatat ttttaaagtc atatatatca tatatagaca
     8641 gaaaggattg ataaaaatgg ctaaaagttt tacagaaata gaaaaagaca atataagaaa
     8701 aagactaatt tcagaatgtg aaatcaactg gtctaaatat ggatacaaaa aaactaatat
     8761 tgatgaactt tgttcaaaat caggaattgc aaagggttct ttctatacat tttttgattc
     8821 aaaagaaaag ttattttttg aagtattaaa tggaatccaa aacagactta tatcatcagt
     8881 agatgaaatt ttaagtaaca tgcctccaaa agaaggcttt gcaaaatcct taaaaatgtt
     8941 atatcgatta tatgataaaa atccattttt atattcacca aataatcctg atttcatcag
     9001 cctaataaat aaactacctc aagatatgtt tttacaactt gagtccaata acatacaaag
     9061 tttttacgac ttaattgaga gacacaatct caagttgaag atagaaaaag aaaaagtttt
     9121 tggtgtttgt aatgctctac tatcaagtct gttggtaaaa gaaaatattg aatataacta
     9181 tattgaagtg tttgacttta tggtggataa tcttgtagaa cacatacttg aataacaatt
     9241 taaaataatt tttctaagta tttttttata ctaaatagga ttaatatgat tgaattaatg
     9301 caaaaaataa acaatataat ttataaaata aatgataaac taaatatcac acaaaactta
     9361 tagaccctag agcaaaccca ttccactcta ctctaagcca tctataatag tttgtatatt
     9421 tctaataata ttagatatta aagtaagaaa aaaattcctt ataataattg ctattatatc
     9481 aattattgta aatgttttaa taatcttaga taggtaggta catatatggt aaaatcctcg
     9541 aacactttta ataaaaactt caacttgatg atattaggtc aaataatctc attgtttggt
     9601 gcatcaatac ttaaatttgc tttatcttta tacatacttg atataactgg taaagcagaa
     9661 atatttgcta ctatactagc agtatcttca atcccaatta tagtattctc ccctattggt
     9721 ggagctattg cagatatatt caacagaaga aacttaatgg ttatttttga tttttcaagt
     9781 agtcttacag tactaatctt agcagtattc ttattcaaca ataatggttc aatattactt
     9841 gtaggaatta ttatgaccat attatcagtc ataagcacaa tgtatcaacc aactgttcag
     9901 tcaagtacac cccttttagt tgataatgag catttaatga atgcaaatgg tattgtagct
     9961 ggagttgcat ctcttacaaa tattgcagga cctgtattag gaggtgtctt gtatggtgtg
    10021 attggaatta atgcaattat agttataagt tgtgtaagtt tctttctatc tgcaattatg
    10081 gagatattta tacagattcc ttttatcaaa caagagcaaa acagcaacat tataaaaact
    10141 atatttactg atataaagga tggtattgtt catatatgta aagaaaatag atttatacta
    10201 aaaatacttt ttttagctat agcaataaac ttatttttaa gctctatgtt aatagttggt
    10261 atcccctata tagtaaaaat cactatggga gctagtaact tcttatatgg tctagcagaa
    10321 ggagcaattt cttttagttc actggtagct gctgttagta ttggtatttt ctcaaaacat
    10381 ctaaaaatca ctaatttgca tatttccttt attatatgtg cagttatgtt cataccaatg
    10441 gcggtttcag tatatccatt tatattaaac acaggatttc cagttccatt tttaatcttt
    10501 atagtatcat ctatgctaat attttttaca acgactctta tctcaatata tgttataaca
    10561 gtcacacaat taaaaactcc aaatgaactc ttaggaaaag taatgtcaat tctttttgca
    10621 ggaacagcaa ttgcaatgcc ccttggtcag gttatgtatg gtaaactttt tgatttatta
    10681 tcagatagag tgtacatcat tatactagga gttggattta ttacattact tatagcattt
    10741 gtatcaaaga taacattgag gaacgaaaat ctttagatac aaagaattta gatataaaga
    10801 tttaattttc actaatttct gaatttaaat gcaaaattgt gtatcagtat gttgacgttt
    10861 tagaaattga ttgatataat caatggaaaa taaactttat aaaaattcaa aatattcagc
    10921 tagaggagtg ataaaaatga gttacatgcc aactttagaa caagcatggg aaatattaag
    10981 aaaatacaat aaggaagaat ttcatataag acatgctcaa attgtatctg gagttatgag
    11041 atactatgca aaggagtacg accctgaaag agtagaattt tgggaaatag ttggactttt
    11101 acatgattta gattttgaaa tgtatcctga tgaacattgt gttaaacaaa gagaattaat
    11161 gacagagctt gatttagata aatcaataat cgattctaca ataagtcatg gatatggatt
    11221 aactggttca gatgttaaac cagagcaatt tatggagaaa gtattatttg ctgtggatga
    11281 acttactggt ttaattggtg ctgctgcaat aatgcgtcct tcaaagagcg tttctgattt
    11341 agaattaaaa tctgttaaga agaaatttaa agataaatca tttgctgctg gctgttctcg
    11401 agacgttata agaagtggtg cagaaatgct tggatgggaa cttgataagc ttatccaaac
    11461 aactatagaa gctatgagaa gcttgattcc agagatggca atatagctcc tccatacata
    11521 taccatagaa gttacatctt taaagagcat ttctcttttt gatgcaactt ctttttatat
    11581 tttgtagaat aaaaagttta atattttaaa ctcactatat aataaataat ataatgtata
    11641 atagtcataa actttctaac cttaaaagtt tctaataatc aataaacaat tctaatatac
    11701 aaatgtcatt atatgattta attatatatc ttcaatttat atattaaaat aaatatatat
    11761 atattaaatt cttatacacc aaatttttac attaaacttc tatgcaccac atttatacat
    11821 attaaattct tatatattaa atttctatgc atcactttta tacatattaa attcctatat
    11881 attaaacttt tatgcatcac ttttatacat attaaattct tatatattaa acttttatgc
    11941 atcactttta tacattagtt cttatatatt aaacttctat gcatcacttt tatacattaa
    12001 ttcttatata ttaaacttct atgcatcact tttatacatt aattcttata tattaaattt
    12061 ctacacatca aatttatata catttaaatc acatgattaa ctgctttctt cacatttcta
    12121 agtggaatag caattttaac tacaaatttt ttatcatcat attcaattac aacattacca
    12181 ccatacttat ttacagagtt ttttacactt tttattccaa gtccatgaag agaacctttt
    12241 ttgtctgtca aaatatctcc atttttagta ataatcttat ttatcttagt attttcaact
    12301 cttattaaat aaaaattttg cataacatca cctttaacac aaataactct atttccacta
    12361 tcaattttat tacatgcctc aattgcattg tctaaaatat ttgaaaatat atgacatata
    12421 tccactaatt caacagcttc acaacaacta aaatttattc ctatttttag ttctatatca
    12481 ttcttatcac aaacctcttt cttttcactt aatatagcat ctaatataaa attaccagta
    12541 ttaaataaca agtcattact ctgaatttct acctgcaaat tatttaaata agaagtaaca
    12601 tcaatacctt ttttaatcat agcatttata cacaatatgt gatttttcat atcatgactt
    12661 agtaatctag tttttaaata tttcttttct atatcttcat aatactcatt ttcatacttc
    12721 aaagtatttt taatcatatt gttttctaca actaaagtat tatattgctg taatttttta
    12781 gcaattaaaa ctaaagtgat acttgatata agcaatacaa cagaaatcat aacgaattgg
    12841 aaattatgta ttaagctacc agaccctgga ttaaatatgt attcaaaaat tacaaaaaaa
    12901 gatgctatat tagcgactat aggaataact atataaaata atgtcctttt atttatttct
    12961 atattaagtt ttatctttaa ggctctatat aaaagcaaag acacaatcaa aagtatcttt
    13021 cccaatatta tagactcaat tctgtatata tctcccgtcc ccatactact catgtcattt
    13081 atagaattaa gccaaactgt taaagatata ctcaaagcat ctgtggcgat tagtaacatc
    13141 caataaatta aggatattaa tacagacttt ataaacccca catcatatga gattttataa
    13201 aatgcaatag taattatcat agatataatt actcttatat ttggaggtac atcaaaaaaa
    13261 tatattatgt cagcaaaaac aataatacct accatacaaa agtttatttt tttgttagaa
    13321 atccttttct cacttgtata atctagcatt ttcttacaaa gaaaccattc tatcataaag
    13381 gatatcgcaa tgaaaatatt ccaaaataag cttgacttta tcataatact ttctcctaac
    13441 gtattactaa attatttttt tatattttat gtataattta attatatacg actctacaca
    13501 tgtagtaaat agattgtcaa aattattcca aataagtcca tatttctttt cttttttaat
    13561 tatatcacta aatttgctaa attgaattaa actttttatt gcttttttta ataataacta
    13621 tataaattta tctataacta ggtataaaat atattattta taaagttttt acattatttc
    13681 tataataaaa tcttataaat attacaataa aatatttttt tccatgatat accttgataa
    13741 aatctctgct tctttaagca gtaactttga gttgaatcat tatagtgatt tataacattt
    13801 atagaaatgc tgttatagat ttatatttaa acactacaaa tggaaattta actattataa
    13861 aaaacaatca ttagataaaa tgattcttac aacaaaagca tttctattgt agatttatta
    13921 gtaaataata aatctacaat agaaaaaact agaagtacag tatcataaaa atataattaa
    13981 ctgtacttct aaattattgt ttttaaaata aattttcatt cattaaaaaa tacatataaa
    14041 tatttccaga ctccgattca gcaaacttat ataagttcac tttttattta tcatcaatta
    14101 ttttcatata cttcttttat ctcatcttca cttaagtcaa aatcattaaa tttatttaat
    14161 atgtcatcta atttaaaacc attttttttc aacattataa ttattttata atctttttct
    14221 tttgcaactc taattgctgt ctctctttca atctcttctt taaaatttct acttttattc
    14281 atgatataag cacctctttt cagatttaat aaatattctt cattgctacc taattctttt
    14341 aatctatcaa gtgctttttt aataatagaa tcattcttag ctataactct tacatctaag
    14401 ttagaaggat tactaataag ttttccccat agcataattc tatttactat atagtcagat
    14461 ttatgaattt ttggaagctc tataaaatat aatgcagttt tactagaata aatatctgga
    14521 tgcccgtcca ttgttaatct caggcaattt acataatctt ctatgtgatt atattttaca
    14581 tgatttagta tatgtattcc aatagttttt ccaatttctt tatactcttt accttcttct
    14641 aactttctac catgtacacg actaagataa tattcagctc tttccaaata atcgttctca
    14701 aaagctcttt gcatctcaat atttatatga ttattttctt catcttcagc caaaatatca
    14761 agtttaggtc ttttctctcc aatgtaatta attgttggat cagtttcaac tctaattatt
    14821 ctctttattt gagtgaacgg tttgatattc ctgttagcat atggagcatc ttttagaata
    14881 tcatttataa aatttacaag taaatcatat tcattattta aagtgaacaa tgatttaaat
    14941 acctcatcat actcaggctt agcatatcca tcttcagtca caacttctcc aacattttca
    15001 ttagtcaaaa tctcttttgg aattaatgcc ttgattttat ctaattcatc attagaaaaa
    15061 ctacttctac taagtatatc atttaatttt ttggtccatt ctttatcttt ttcttcaatt
    15121 atagttcgct tatattcttt tgatgtttta ataatcttag atttttcttc tactttttct
    15181 atcatttcaa cacctctctt tttcctcttt tgtatttatc atttcttgaa attaaactaa
    15241 gtcactacct cttttaaaag ttcttgctcc ataaacactt tcttaaactc taatgtctgt
    15301 gtatgtactt gacattctct ctctatctaa agttaaataa attaatctat gtatagagga
    15361 taagtccaaa aataacataa aacactaatt aatcatttat aaagcattaa ttaatcactg
    15421 acaatgctgt tattgtcacc acatagcacc aacatagatt cttctattat agggcaatta
    15481 tctatgaaaa atagactcat aagattagaa aatgtactta aattttagtt catttaaata
    15541 ttcattttga agaaagaaat ttaactttag actaaaaagg aaatatttca agaggattaa
    15601 gaaattttct taaagggata ataattttta tgaaaatgga acaaatatga aaataaagtt
    15661 atgattttta cagccataac cttaccaaca agcacttaat tatcactcaa aaaataaact
    15721 tttatttaaa gaatgcacat aaatatttcc atgttcaaaa cacttctatg atatatttat
    15781 taataaatct accatacaaa aaactagagg tacattaatc ataaaaatat gattaactgt
    15841 acctctaatt atactcatct tttcataaat ttaatcacta tgcatcaaca tatcaacata
    15901 ttgattaaac ttatcttata taacaatatc ttagctacta atataatata tattagtctt
    15961 gaataatact ttctcttgat tcaatttctt ttgaaatact agctataaat tcatctaatt
    16021 taatactacc tatttcacct ttatctcttg atctaacaga aacattatta tcagctactt
    16081 ctttttcacc tactattaac atgtatggca ctttttcaag ctgagcttct cttatcttaa
    16141 atccaatttt ttcagctcta tcatctaatt caactcttat acccttatca aataactctt
    16201 tcttaacttc atttgcataa tccataaatt tatcagatat aggaagtatc ttaacttgag
    16261 ttggagataa ccaaactggg aatttaccag cataatgctc tattaatata cctataaatc
    16321 tttctatact accaaaagca accctatgaa tcataactgg tctatgcttt tcaccatctt
    16381 gaccaatata agtattatca aattgtcttg gaagctgcat atctaattgg atagtaccac
    16441 attgccaagt tcttcctaaa caatctttta gatggaagtc tatcttagga ccatagaatg
    16501 ctccatcacc ctcattaata gtatatggta gacctaactc ttctaaagct tctcttaatc
    16561 cattttctgc tgcttcccac tcttcgtcgc ttcccataga gttctctggt cttgtagata
    16621 attctaaatt atattcaaat ccaaaagttt tgtatatacc atctattaag ttagctactc
    16681 cttttatttc gtccttaatt tgctctggta acatgaatat atgtgaatcg tcttgagtaa
    16741 atgctctaac tctcattaat ccttgtaatg caccagataa ctcatgtcta tgaactctac
    16801 caagctctgc aactctcatt gggaaatctc tatatgaatg tagttgagag ttatagtaaa
    16861 ttaatccacc aggacagttc ataggtttta tagcataatc ctcatcatct attttaactg
    16921 tatacatatt ttctttgtaa tgataccagt gtcctgaagt ctcccataat tttctattta
    16981 atatgattgg agattctatc tcaatatatc cagcttttct gtgtatttct ctccagaatt
    17041 ttaataattc atttttaagt tccatacctt ttggtaaaaa catagggaat cctggacctt
    17101 cttcaggtat cataaataat cctaattctt tacctaattt tctgtggtct ctctttttag
    17161 cttcttctag taaatgtaag tactcttcta agtctttgtt tttctcaaaa gaagttccat
    17221 atattctttg aagcatctta ttattttcgt caccacgcca atatgcacct gctacacttt
    17281 gtaatttaac agcttttacc tttttagtag atggtaaatg tggtcctcta cataaatcag
    17341 taaaatcacc ttgcttgtag aaagatatta tttcactttc aggtaaatca gaaataagtt
    17401 ctaccttata gtcttctcct tgttctttca taagctctaa agcttcattt ctaggaagtt
    17461 caaatctttc tatttttaaa tcttctttag ctattttttt catttcagct tctattttct
    17521 ctaggtcctc tggaactaat ctatggtcta aatctatatc atagtagaat ccattttcta
    17581 tacttggtcc tatagcaagc tttgcttctg ggtaaagtct ttttatagct tgagctaata
    17641 tatgcgctga tgtatgtcta aaaacttcct taccttcttt atcttcaaac ttaaataaat
    17701 tcaagtcaca atcagtatct ataatgtggt ccagacctac aacctctccg ttcacagacg
    17761 ctgctaccac atttctagct aatccttcac taatggattt agctacatcc ataacagaaa
    17821 tagcattttc aaattctttt attgagccat cttttaaagc aactttaatc attgtttttt
    17881 cctccttatt gaaataattt ttagattttt tgtaataaaa aatcgcccca aatatgtttg
    17941 ggacgatatt aattcgcggt tccacccaag ttggcgtata caagtatata caaccctctt
    18001 gatgactata aggtcgtcac ccgagcactt tcacgtatag tataaataca aatactgcta
    18061 ataagctcag ctccaaggta gttttcaaat agacctatta catgggctct cagccataga
    18121 cccaaattct ctgagaatgg tttctactct actcttcctc ttcaacacta tgatttttaa
    18181 tatattttca ttataacaga tagttataca tcaatcaaca ttaatgtaat atattaatta
    18241 cccattattt ttcatctcaa tcagctgtaa accaatattc ttatattttt atattaaatt
    18301 aatctcttga tttatagtta tattataata ttttcataaa tttaaaattt ttaaaacgta
    18361 tccacatgca tgatagatat gactagataa atactaaaac taatttttat taaaactaac
    18421 ttctaatttt tgtactcttt ttaggttttg tatacttaaa tacaatcaat acttaatatg
    18481 gttaataagt agtacagtaa atacttaaat actattaata agtaacacaa tcaaataaat
    18541 aaatacaatt aatatctaaa tgctattaat aagtaattca ctcaaatagg taaatacaat
    18601 taatatctaa atactattaa taagtaattc act
//
LOCUS       DAELTA010000076        18470 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.076, whole genome shotgun sequence.
ACCESSION   DAELTA010000076 DAELTA010000000
VERSION     DAELTA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.076"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      41..143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            237..1613
                     /locus_tag="KPG13_002148"
     CDS             237..1613
                     /locus_tag="KPG13_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG0985863.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1727..2872)
                     /locus_tag="KPG13_002149"
     CDS             complement(1727..2872)
                     /locus_tag="KPG13_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0985864.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNQSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3125..3832
                     /locus_tag="KPG13_002150"
     CDS             3125..3832
                     /locus_tag="KPG13_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985865.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3879..5474
                     /locus_tag="KPG13_002151"
     CDS             3879..5474
                     /locus_tag="KPG13_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985866.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5577..6659
                     /locus_tag="KPG13_002152"
     CDS             5577..6659
                     /locus_tag="KPG13_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG0985867.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6808..6969
                     /locus_tag="KPG13_002153"
     CDS             6808..6969
                     /locus_tag="KPG13_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG0985868.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7310..8578
                     /locus_tag="KPG13_002154"
     CDS             7310..8578
                     /locus_tag="KPG13_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0985869.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8773..9561
                     /locus_tag="KPG13_002155"
     CDS             8773..9561
                     /locus_tag="KPG13_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0985870.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9561..10010
                     /locus_tag="KPG13_002156"
     CDS             9561..10010
                     /locus_tag="KPG13_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG0985871.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10014..12278
                     /locus_tag="KPG13_002157"
     CDS             10014..12278
                     /locus_tag="KPG13_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0985872.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12418..12729
                     /locus_tag="KPG13_002158"
     CDS             12418..12729
                     /locus_tag="KPG13_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985873.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            13015..13515
                     /locus_tag="KPG13_002159"
     CDS             13015..13515
                     /locus_tag="KPG13_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG0985874.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            13572..14558
                     /locus_tag="KPG13_002160"
     CDS             13572..14558
                     /locus_tag="KPG13_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0985875.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            14608..15330
                     /locus_tag="KPG13_002161"
     CDS             14608..15330
                     /locus_tag="KPG13_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG0985876.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            15359..17293
                     /locus_tag="KPG13_002162"
     CDS             15359..17293
                     /locus_tag="KPG13_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0985877.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            17286..>18470
                     /locus_tag="KPG13_002163"
     CDS             17286..>18470
                     /locus_tag="KPG13_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG0985878.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKF"
BASE COUNT         7052 a         1821 c         3153 g         6444 t
ORIGIN      
        1 aaattaatta tataaattaa aataatattc gtaaagaaaa attcatgttt tgtactgtat
       61 aacatataat tttgacaata tgggtcataa gtttctaccg gaataccgta aatattctga
      121 ctatgtatac tattatttag ttaattaaat atttaattaa ctgaattatg aaggatacgg
      181 ctcagcttat caactactgt gtcgtttttt tgtaaaattt aataggaggt gttgttatgc
      241 aaacaacaaa cagtagtact atgcagaaac tttttccaat tttgactaat aaaaatgtcg
      301 acatgaagaa ggaaataatt gcaggagtaa ctactttttt aactatggct tatataatag
      361 ctgtaaatcc aaatatttta tctgagacag gcatgcctgc aggtgcactt gttacaggta
      421 cttgtttagc agcagcattt ggatgtattt taatgggtgt agttgctaac cttccttttg
      481 ctttagcatc aggtatgggt cttaatgcat tttttgcata tacagtagtt ttacaaatgg
      541 gtgttccttg ggaagtagct cttacagcag tatttgtaga aggtattatt tttatagtat
      601 tatcagtaag tggagttcgt gaggctgttg taaatgcgat accaaaaaat atgaaattag
      661 ctgttacagg tggtatagga atatttattg cacttatagg tctagttaac agtggaattg
      721 ttattggaga ccaagccact cttatcaaaa tgggtagatt tacaccagca gtaataataa
      781 cttgtgtagg attaataatt attgctgtat tagataaaaa aagagttaaa ggttctatac
      841 tttttggaat agtagtgagt tcattacttg cttgggcatt tgcttttata aatccagagt
      901 atgcacaaaa attaggtata tatttacctg gtggattatt taaattcgaa agtttagcac
      961 caatagcagg taaaatagat ttaggatatg tactacatcc aacaaatata ggtggttttt
     1021 tagtagtagt ttgtacattt ttatttgttg atttttttga tacagtaggt acattagttg
     1081 gtgtttgttc taaagcaaac atgttagatg aaaaagggaa tgttcctaat gtaggtagag
     1141 ccttacttac agatgcggtt gcaactacag ttggtgcagg acttggagta tctacagtta
     1201 caacttatgt tgagagttca acaggagtta tcgcaggtgg aagaactgga tggacagcaa
     1261 taacagttgg aattttattc ttagctgcaa tgtttttctc tcctgtattt atagcaattc
     1321 catcatgtgc tacagcacca gctttaatat atgttggata tttaatgctt ggtacagtta
     1381 aggatataga gtttgataat ataactgaag gtgttccagc ttttgttact attgcttgta
     1441 tggcattaac atatagtata ggtgatggtt taactttagg tatattaact tatgttttcg
     1501 taaatatatt ttataatata tttggagcta aaaaggcaga agacaaaaaa catgtttctg
     1561 tagtaatgat tgttctggca atattattta taataaaact ttggctacta taataggtat
     1621 tatttaaaat tttaatttaa tataaaataa agggctgtaa tttattatca attgtgatga
     1681 taaactacag ctcttttgta tttgttgtat ttttgtattt attgtattat ttaataatgt
     1741 ctattagttc tccattttgt aaagttttag gtgtaattac tatattgaac tctttttgca
     1801 aatcttttat aatatctaat tctttttttg ttattattga gattgtgtta ccactttgat
     1861 ttcctctagc agttcgacca catctatgca ggtattcatt tttacttttt ggaaaatcca
     1921 agttaaaaac atgacttaca tccacaatat ctagtccacg agcagataaa tcagttgtaa
     1981 ttaaaatctt tgctttacca agtttaaatt tatttattgc attctttcta tcttcttttt
     2041 ccatatttcc aaaaattcct attgctttgt aattatgata atttagtttt gatactagga
     2101 cttctatatt tttttcatta tttacaaata caatagcttt ttttggattt gtagctgcta
     2161 atgcctttct aagaaaagta aatttatccc ttatctcacc taataaatat gagtgattga
     2221 tgttaggatt tatttgtgat ttttcctttg tttttattat ttcaaactcc ttcataagtt
     2281 tatcacaaat ttttatagtg ctatcagtaa gacttgcaga gcatcctata atttgtctat
     2341 ctcttaacgt tgttcttatt atgtcttcta tacaagttat attttttcca ttcaatagat
     2401 tgtccacttc atctaagata atagttttta tattatgtga ttttagtttc ttttgtttta
     2461 tcaagtctaa aactcttcca caagagccaa ttactatgtg tggttttact gcttttatat
     2521 tttttatttg tttttgtata ttgacctccc caattaaagc taaagaagta acggagagtt
     2581 ctgcattttt agccaataat tcaacctggt ttgtaatctg cattactaat tcatgcgtag
     2641 gtgcaagtat aagagcttgt gtttctcttt ttgatgtatc aattttttca aatataggta
     2701 aaaggtatgc tagtgttttt cctgttccag tctgtgaatt aatcaataaa tccttatttt
     2761 gaattatatt ccctataaca agtgactgaa cttctgttgg agaagttatg tcttgttttt
     2821 tgagtccatc tattagagtg gaacttattt ttaattgttc aaatgtattc atattttact
     2881 ccttatattt taataattat agtcaactat tttttattat acattgttta ttcaaatcga
     2941 actatatatt cttttataat tatgtgtaat ttgaaagtaa atatcaatta tatttatacc
     3001 tttatatttc tgataccttt gcattttata gatttctaaa ataaacaaat aaaagttgta
     3061 attgtttgat atttgttata tattatatat aacaaatatt aaaactataa tatggaggga
     3121 aattatgtta aaagtaatca atgtatctaa atcatataaa aataaaaaag tagtaagtag
     3181 tgtctcattt gatgtaaatg atggagagct ttttgggttt ataggacata atggagcagg
     3241 taaaactact actattaaag caattgttgg aatacacgat tttgaagagg gagaggtact
     3301 aataaattct aaatctataa aaaaacagcc tgttgaatgt aaaaaggaaa tggcatatat
     3361 acctgataat ccagatttat acgagtcatt aactggtata caatacctta attttatagc
     3421 tgacatattt gaagttgcca aagataaaag agaagagtta ataaaatatt attcaaataa
     3481 gtttgaactc aataaagcat taggagattt aatatcttca tattcacatg gtatgaaaca
     3541 aaaattagct ataatttcgg ctttgataca cagcccaaaa atcttaattc ttgatgaacc
     3601 ttttgtaggt cttgacccaa aagcttcctt tatattgaag gaaattatga gggagttttg
     3661 tgacaaaggt ggttgtatat ttttctcaac acacgtttta gaagttgctg aaaaaatttg
     3721 tgataagatt gccataataa aagatggaaa tataattgct tatggaacta cagaagaagt
     3781 taagggaagt aattcacttg aaagtatctt tatggaatta atagagaaat aaaatgtttt
     3841 ttatacaata tagtgtctaa agtgaggaat gataaaatat gagcaatcta agtatattaa
     3901 taaaaactaa tataataaat gaatttaaat taaatgcgtt aaaaaaagct gatggtagag
     3961 aaaaacaaaa aataatggga atgatgcttt cagttgttat aatagcgggt ctattaattt
     4021 tttatgtaac aagcttaagt ctctctttag ttgatatctt aaagcaaata aatcaactgg
     4081 aaatgctact gatatttggt tttggattat caacattaac aactattgtt acatcattat
     4141 ataaaacgtc atcatatcta tttcaagcaa aagatttaga ccttttgact agtttaccta
     4201 taaaagaatc tactgtacta gcaagtaaga tattaatgtt ggttggagca aactatttat
     4261 tttcttcaat atgtatgatt atacctgcag tggtttactt tttaaattct gatgtaagta
     4321 taacttattt cccttatttg atattgctat ttttagcttt accattatta ccgatagttg
     4381 cttcatcaat aatttttcta tttatagggc agatatcatc aagaataaaa tacaaaaatt
     4441 tagtattaat cataggtagc attatattga tgttgcttta taccctactt atgtcaagac
     4501 ttaattctat gtcagttgaa atgctaaaga atagtgtgtc tatttcagag acaattagaa
     4561 aagtatatcc tcctatatat tattttgttg atgctcttaa aacaggaagt atagtttcat
     4621 ttttagcatt tatagccatc tcaataatac cattttttat gtttgtaact ctatttgcaa
     4681 aaaaatttaa agatataaat gctaagatga gtgaaggata taagtcaaaa aattatgaat
     4741 ttaagggtca aaaaacttca agaccttcaa aagctttatt aaaaaaagaa tttaagagat
     4801 atacttcttc gtttatatac tttttaaatt catctgcagg tttgttattt ctaatggtag
     4861 gtacaatttt aatattatta tttggagcag ataaagtggg tgagttactg aaattgaata
     4921 tagattttaa tcttataaaa atacaattat tgggagtatt cttctttata atagtaatga
     4981 attgtacaac atactgttca atttctcttg aaggaaaaaa cttatggata ctgaaatctt
     5041 ctccaataga tgaaagagaa atattctggt caaaaatatt gatgaatttt ttattgaatg
     5101 caccactatc tattttatgt ctaatattag tatctataag attagacttt gaaattcagt
     5161 ttattattgt atcaatctgt ttgattgtaa gtttttcaat gtttgtagca ttgatgggat
     5221 tattatcaaa tttattatac ccaaaccttg attggaaaaa tgaggtggct gtagtaaaaa
     5281 gaagtgcaag tatgatagtt acaatgttag tatcattagc ttatgtaatt atacttggag
     5341 gtatatatgt attacttgga atttcgtttt taaatatata tattattttg gcaagtatac
     5401 ttactttgat attagatttt atgatatgga gaattatttc tactaaagga gtaagaatat
     5461 ttaaaaattt ataggtgtat ttatgtcaaa taaagactta tcttttttag tcttgcaaat
     5521 aataggttaa gtagtatact atgtatagtg atatgaaaaa atttaggaga aaataaatga
     5581 gaaaattatc caaaagagaa aaagatttta ttcaatatgg atttttatta tttttgatat
     5641 gtattactac ttatttagta ttaactactt tagatataaa actaatacct aaaattataa
     5701 ggctagtaaa taagacttat atatttcttg gcatattgac aattatagta tatatgataa
     5761 ttgaatctat tgtaactcat ttgataataa atgcagttca aaaaacaaaa gtaaaattta
     5821 ttggtattaa aatggctaca atgggatttt actataactt agttactcca tttgcatctg
     5881 ggagtcaacc aatgcaaata tatgcactta caaaatatga tgtaagcctt agtaaatcag
     5941 tggctatagt tactaataaa actgtagtat ttcaaagtac agttactgta ttttgtggaa
     6001 ttttaatttt gctaaataag tcattactga tggagcaagt acattctgtg aaagtcttga
     6061 taacttttgg aatgattatg aatgtaagta tgttgttagg tggactacta atagtattta
     6121 gtccaaaaca agtaaaaatg atagcagaag ttttgattaa ttttttatct aaatttaaaa
     6181 catttaaatt tttagattca aaacgtgata gaataaatca ttatattgat gattataatt
     6241 attctataaa aatatttatt aaaaatgtaa gaatcttagt tttaagtata ttacttactg
     6301 tgttacaatt aatcgcatat ttcagtatag tttattgtgt gtataaagcc tcaaatttaa
     6361 gaggagtttc atatgtacat ataatgacat tacaggtctt tttatatatg gctatatcac
     6421 caattccaac accaggaaat gttggagcta atgaggttac attttttact atgttctcca
     6481 atataattcc aaaagaatta ttggggtact ctgtactctt atatagtggg tttgtatact
     6541 attttatatt aatagtttca ggtttattta catttggaac gcattataca ttaaattctt
     6601 ggaaaaagga aaataaagta aattctaatt tagataaggt tattgaaaaa ttaaattaat
     6661 aaaataatta gggaaattct atatataaat atggtgttga ataagctata ttatattgag
     6721 atatttccct tttttgatta aaattataga aatttgaaat aataagtaat aaacagaaaa
     6781 agtgatattg ttttgaaagg agaaactatg aaacaagata aacaaaatga aaaacaaagt
     6841 gagatttcta agataaaaca aagcaatgat aataaacttg aaatagctca agaattacat
     6901 gtaagtaaaa atatagggaa tatgcttgga tatagaatag ataagaaaaa aatgaataga
     6961 ccaagttaat gaaaatgttg aaaatggtac attttaaaaa atatatataa tgaaatgttt
     7021 tattgttaaa tttaaactta ttttaatggg ggaaaaagtc gaaaaatatc taaaaagata
     7081 tggatataaa aaatattaat atatatatac tataaacttg acaaaatcaa ccttttaata
     7141 actagcaaaa aatagcaaaa ttatataagc gttatttaat tgtcaaaaat gaaagctaaa
     7201 ttgcatgtcc taaaaaaaat gattgataac atttatttgt gtgctataat tatatagtaa
     7261 aacaataagt atattgcata atgtatgcaa atcgggagga gagacggaca tggaagcatc
     7321 tatacacaca attgccagca ataatttatt gataattttt gctattgtaa gtataactgg
     7381 aataatttgt agtaagctta gtgaaataat aaaggttcca gatgtagtgt tatatttact
     7441 tgtaggtatt ttaataggac catcattttt aaaatttata gatattcgtg gatttcaaat
     7501 agaaaatcaa ctaatactta catttggttc tgcatttatt ttatatttag gtggaaaaga
     7561 aataagttta aaagtattga gaaatgtgaa gatatctgta ttcttattag ctactttagg
     7621 agttgtgata tctgctttta taatgcaaca agtaatagga tttacttttg ggataagtgc
     7681 aatgactgct ttactagcag gaactataat agcatcaaca gacccagcca ctcttgtacc
     7741 tatatttaat caggtaaaga taaaggatag ggtaaaacaa actgtaataa gtgaatcagc
     7801 ttttaatgat gcaacaggag ctatactaac atcagcagtg ttggctgtaa ttttgtcaaa
     7861 taaattttca ttaggtgaaa atatttatga actggggaca atggttattg tcggagtttt
     7921 agttggttta attacaggaa tactgctttt gaaattagtt aatgataaac cttatggaat
     7981 atttaaagat tttgcaccaa taatatctgt tatttcagtt gtagttgcat atgaattatc
     8041 tacaaaatta ggtggaagtg gatatatgtc ttgctttatt gtaggtataa taactggtaa
     8101 caagaaaaat tttaaaattt ggttaagtca aaaatcttat gatgcagatt tttatgttgc
     8161 tgaaacttta ggtactattt gtcgtatggc aatatttata atattgggta gccaagttaa
     8221 tttggctcag ttaagtaaat attttatgcc atctgtaatt acagtattag tattaatgtt
     8281 tatagcaaga ccactttgcg ttttagtttg tacacttgta gacagggaag caaaatggag
     8341 taaaagtgaa atattattta tgatgtgggt aagggaaaca ggcgtaatac cagcagcact
     8401 ttgtggtatt atttcagcaa tgaaagttcc tggatacgaa gtgatttcat ctgtagtatt
     8461 catgactata ctaattacat tagtaataca aggaagtact acaaaattag tagctaaaaa
     8521 acttggatta ttagaagaag aagtgataaa tataagtgaa aaagtatcaa ctttttaaag
     8581 ttttgcttta aaatagtacc tgttaatggc tattttaata ttgtctaatg atgattattg
     8641 aaatagtcat ttttttatgt ataagtaatt tacagaattt acatattaga catatttatt
     8701 atcaaaattt gttgaagcta tgatataatt agttgtattt aaaataagaa tagcaaataa
     8761 gggaggaaat ttatgtttac ggttatggat attgtacagc cagatacagt agaagaagcg
     8821 tactcaattt taaataaaag aaaaactaac caagtaattg gtggaagtgc ttttttaaga
     8881 atggggaaaa aaagaatagg aacaggaata gaattatcta aattaaattt agattatata
     8941 aaagagtatg aagattacgt tgagattggt tctatgacta cttttagaac attagaaaca
     9001 agttccataa taaaaaataa ttttggaaga ataatagaag attcagttaa aaatataata
     9061 ggagttcagt ttagaaatgt agtaacagtt ggtgcaacag tattttctaa atatggtttt
     9121 tctgacttga tagtagcatt actttcatta gatacggaag tagaattata taatattggg
     9181 agaattagtc ttgaagagtt tctaaataga gattatgaga aagatttact tattaagata
     9241 tacataaaga agacaaataa gaatgcttct tataaatcaa ttagaaatgc aaagagtgat
     9301 tatccaattc taaatgtatc tgtatcaaaa catatggaac aatttaagtt atgtgtagga
     9361 gcaagaccgc agaaagctac gattgcaaaa caagctagtg aatttctatc taacaatgaa
     9421 atcaatgaaa ttaatataga taaagcagta gaaatagcat cagaagaact aacttttggt
     9481 tctaacatga gagcatctag ggagtataga aaagctatga gtaaagtact tttaaaaaga
     9541 gctataatgg aggttattta atgttagtaa agattaatat aaatgggaag aaaaaaagtg
     9601 ttgatataga accagaagaa tatctggttg atactttaag aaaagttggt aatttaagtg
     9661 tgaaaagagg ttgtgacaca ggttgttgtg gtttatgtac agttttaatt gacaagaaac
     9721 ctacattatc ttgtgcaaca ctgacattga gagctttaga taaagaaata actacaattg
     9781 aaggtttaga aaatgaagtt aaagaattta gtgaagtttt ggtaaaagaa ggagctgagc
     9841 aatgtggttt ctgttctcct ggctttatac ttactgtact tgcaatgaag gatgagcttg
     9901 aaaatcctac agatgatgaa ataaagcatt atcttacagg aaatctatgt agatgtacag
     9961 ggtatatggg acagttaaga gctatcaaaa cttatctgga ggtaaaatag aacatgaata
    10021 tagttggaaa aggaatcaag aaaatagatg gaatgagtat cacaacagga aagcctgtat
    10081 atacagatga tttatcagca aaagatgctt tggtggtaaa gatacttaga agtcctcacg
    10141 cttatgcaaa aataaaagat atagatacat caaaggcaga aatggttgat ggtgttgaat
    10201 gtgttttaac atataaagat gtaccaaaat ctcgatttac acttgctggt caaacatatc
    10261 cagagccatc tccatatgat agattaattc ttgaagatgt tgtaagatat gtaggtgacg
    10321 aagtagctat agtggcagct atagatgaaa aaactgcaat taaagcaatg aatcttatca
    10381 aagtaaagta tgaagtactt acacctgttt tggattttga agaggcaata ggaaataaaa
    10441 ctatggtaca tgatgaagat agacattgta actttgatat aggtatgcaa agagaaaaaa
    10501 atatagtatc ccatcatgat tatacaaaag gtgatgtaga aaaagtatta agtaattgtg
    10561 atgtagtgat tgaagaaact tattatactc aagctcaagc acatgcaatg atggagacgt
    10621 atagggcata caattattta gaccatatgg gaagattggt tgtagtaagt tcaactcaaa
    10681 taccatttca tgtaaaaaga catttatctc gtgcactaga aataccagct agcaaaatta
    10741 gggttataaa acctagaatt ggtggcggat ttggtggtaa acaaactgca tgtgttgaga
    10801 ttttttctgc aattgttact ttaaaaacag ggaaaccagc taaaataatt tatgatagaa
    10861 aagaaacttt taattgttca acaagtagac atgcaatgag attaaaagtt agactaggtg
    10921 ctacaaatga tggcataata aaggcaatag atataaaagc actttctgat acaggagcat
    10981 atggagagca tgcttctaca actattggtt tggttggaga aaaaacttta ccaatgtata
    11041 ataaagttga agcaacaaga tttattgcag atgtggtata tactaataag atgccagcag
    11101 gagcacttag agggtatgga gcatcacaag gatgttttgc tgttgaatca actataaata
    11161 tgcttgctaa taaattaaaa atggacccaa cagatttaag gttaaaaaat gtggtaacag
    11221 aaggtgaaac tacatttgca tatggtaaaa ctttaaatag tgtcgattta ataaaatgta
    11281 taaataaagg caaagaactt attggatgga atgaaaaata tccttgtaaa catcttgata
    11341 atggaaaaat tagaagtgtg ggtatggcaa ttactatgca aggttctggt atacaaggtc
    11401 ttgataagtc ttcagctgaa ataaaattga atgactgtgg agattatact cttatgatag
    11461 gttctactga tatgggtaca ggaagtgata caattttagc tcaaatggct gctgaggttc
    11521 ttgatacaag cattgaaaat ataacagtta tatcagctga tactgactta gttccatatg
    11581 acccaggttc atatgcttca agtactactt atgtaacagg tatggcagta gttaaggcag
    11641 ctcaagactt aagagaaaaa ataatttctg ctggagcaac taaactaggt gttgagaagg
    11701 atgaagtaga atttgatggt ataaaattaa ctagtaaaaa aggtagcact ttattattcg
    11761 atatagcagt tgattctaca attggtccag atactaatca attaataggt cttgcttctc
    11821 atggtagccc agtatctcct ccacctttta tagcaggttt tgcagaaact gaaattgaca
    11881 cagaaactgg aaaagttgat ttagtaaatt atgtagctgt agtagattgt ggaacggtta
    11941 taaataaaaa tctagctaag atacaagttg aaggtggaat tgttcaaggg attggaatgg
    12001 cattatttga agaagttaga tacacagaaa aaggaagcat ggacacaaat acatttatgc
    12061 aatacaagat tccagctaga tgtgatattg gaaatgttaa agtagatttt gttgaaactt
    12121 atgaaccaac tggaccattt ggtgctaaat ctgttggaga agttgttata aatacacctt
    12181 taccagcaat acaagaagct gtgtttaatg gaattggtgt aagaataaat acactcccta
    12241 ttacacctga aaaagttttt atggaaatga ataaataatt atttatgaaa atttagacta
    12301 gttttacatg ttaaacaacc agttatgtca tacctgttga aagatattta ataagtagtt
    12361 attataatat tatgaatttt acttataaaa tacaaaatta aataagggga tgatgttttg
    12421 gctggtaatc taaataatat gagagcagta aataatttta gaggagataa gaacatttta
    12481 gaatgtttag tcagctttga gggtcgttca ataagtcaga gaaaagtaag ggtatttttt
    12541 aaagaaaaac aaaatcaaat agaaattgat tttgcagaag aggaaatttc taaattggtt
    12601 gaaaatgttg ttttaaatac atcatatcaa gaaatgttat atgatgaaat agagaaacaa
    12661 ctggaaattg attgtatagg tacttggatg atattatcta aattaaaaga tggtagtaga
    12721 gttcactgat tttaaaataa attattagag cctaatttta aggctctttt ttgctgtttt
    12781 taattaacaa tagaaaattt atctttagtt tgtatataat tttaatatca tatattctat
    12841 atatataaaa ttgaatttgt atgtattata ttgtataata taaaatgaat tgggaaaata
    12901 gttcgctaaa ataatttgtt tttataaaat tactgtttaa gtttatagat tttgtataaa
    12961 ataaagttat gttattaatt tttttaaaaa taaaactttt actaggagac agatatgaga
    13021 aaaaatagta ttaatatagc actaactgca gcaattacag caatctatgc agttttaaca
    13081 ttatcattgg gatttataag ttatggacca atacaattta gggtagcaga gattatgatg
    13141 cttttggcat ttttagataa aggatatata gtaggtctta cattaggttg ttttttagca
    13201 aatgtaatag gaccatatgg tgtacctgat ataatttttg gaacattagc cacatttata
    13261 agtgcaagta tgatttatat tactagaaag ttaggtaaac aaaataaaag tactttagtt
    13321 atagcttctt tatggccaac tgttataaat tcgattgtgg ttggtctaat gttaaatgta
    13381 ttctttgggc ttccattaat tctatctatg atacaggtag gttttggaga atttgtagta
    13441 gtaacaattg taggagtacc atttttctgc tttttaatga aaaaatataa aaatgtcatt
    13501 aatattaaat tttaaatagt gtatagtaat ttaattaatt gagagtatta ttagtgaaaa
    13561 gaggtgttta tttgtcgaag gtaaaaagat tgactgatgt ttggatatat attaatgata
    13621 agcaaaagtt tacaataaaa gagttgtcag aggaatttaa tctttcagct aaaacaatac
    13681 aacgatattt aaaagaatta aataaaatgg gacttccaat tcaagcagaa caaggtagaa
    13741 atggaggtta tagggtgcta aataatagct atatacctcc tgttatattt acagaaaaag
    13801 aagttatgat tatactattt gcacttaagt cacttcagat ttataattat aagttaatgg
    13861 aaatagaaat taattctata atgagaaaag taagttatga acgtaaaagc agtataaaag
    13921 taggtataga aaatattaaa agatacattg gctttgtagc taatgatact aatgatggag
    13981 attttaagag tagtaatgta actgagtttt ttagagcttc ttcagaatct ctaattttaa
    14041 atattaagta taagtgtggt aatcagattt tggaaaaaaa cgtatgccct ataggtatat
    14101 atttaaataa gggagcatgg ttttcaccag catatgattg taaaagtgat aaaataatat
    14161 tagttcatat agataatata attgatataa aaagaacagg agaaagtaaa aaaatacata
    14221 taaacttgga agaatggtta catgaagtat ataacaattt atatggaaat agttcaacta
    14281 ctaaaaaatt tggtgaaatt atttatctaa atgtattatt aactaaagaa ggtgtttcta
    14341 aaataaagga cagttcttgg aatttagata tggagctaaa tgaagatggt agtggaataa
    14401 ttagtacatt cataaaggtt gaagatatag attgttatgc ttcgatttta ttttttattg
    14461 gaaataatgc taaggttata gagccaaaat ttttgaataa gctactttat gataaggcca
    14521 atgagttaag atatttttat gaagaaaata tggtataata tttagaaatg ttgaaattaa
    14581 ataattatta aggagatata cgttataatg gaaaatttta ataagaatgc taaaataatt
    14641 ggtattggag ctgaaggtat taatataata aatgaagtag aagaaaaaat aaaagcaaat
    14701 atggatatag aaaaaataaa cataaatcaa gagattgaaa aagaatatgt tagaagtctt
    14761 ttagatggtg tagatatatt atttttgata tattcatctg aagataaaca tattagagat
    14821 attataaaag ctgtttcata tatgtcaaac gaaagaagag ttctatctat aggaatggat
    14881 tgttcagaga aggaaaataa agaagattta gagcttggga gagagtttaa aatcaataat
    14941 gatagtatat ttaaatttgt tgatttaatg aatatcatgg tagagtctat ttctgattct
    15001 tgcatgataa atatagatat tactgattta aaagaagcta ttgtaggaga taaaggaatt
    15061 aagtattctt ttgaagagtt tgaagataca aagtcatata gcgagatagc tgacatttta
    15121 tttgacagga tggaatatat tggtgaggaa tttataagta agaaaggaat tgtatttgtt
    15181 gaaggaagtc cagaattttc tataatggaa ttgaatgatt taataagtaa tattcagagc
    15241 aaggtagaag aaagctatga agtaatattt tctttatata taaaggaaaa tttaaatgga
    15301 aatattagag taggcttatt gtataattag tatattttag atattaagga gagtgtatat
    15361 gtctgaaaat ataagaggta cagagtattg gttaatggaa gaattttttt atcaggaaac
    15421 agacacacca cagattttta gagaagaaag cataattgca cttggatttg atataaaaat
    15481 aaatttaaaa aaagtattca gtatggggaa aaaatcagaa aagtatatta atgagaattg
    15541 taatgataaa gattttataa aaaagtttat gtcgattaaa aaaggagatt attttttata
    15601 taaagcatat caaaatatag aagaaaaagt taaatgtaat gtgtctattg gcatagttga
    15661 agaagatttt gaaaatggat atgaattaag tcatatttta gggcatacac taccagtaaa
    15721 atggatgcat aattttgata cagtttcaaa ttgtaaagta tatacaagtg aattttataa
    15781 attaaataat aatgatatta gttcatattt agaaattatt aagtcacaag ataagaaatt
    15841 attgagaaaa agaatagaag aaagtgaaaa taataaatgg tatttaaaaa actattatta
    15901 taaaaatgta gataagtgtg tagatgatga agaattattt attcagccca ttaatctaga
    15961 acaattagaa agcctaaaaa atataaagac agatgattat attttattgt ataatagcga
    16021 gaaagtaatt tgtgaaaatg accatacaat gaagagtcta tttggcgtat gtcaagttat
    16081 ggaagggttc gataaagaga aagtaagtgc tttggagggt ggatattttt ttcctgttag
    16141 atggatagag tatgaaaagg agaatataga cggaaatgca tgggttatat ttgaaaataa
    16201 tagtatagtt gaaaagtatg taaaaagatt tactaataaa atgccattaa atactatact
    16261 ttatggacca ccaggaactg gtaaaacata caatacaaaa cagatggttt acaatctagt
    16321 aagtgaaacc agtctaagaa acagatacta tacaggttac ttagattata aaaataatgt
    16381 agaattttgt acatttcatc aatcatatgg atatgaggaa tttatagagg gccttaagtc
    16441 tgatggtgaa ggcaatttta agccagaaga tgggatatta aaggatatat ctatagaagc
    16501 atgttataat gcattgaaat ttgaaaaaaa gatgagcttt gagttggaaa aagatagctt
    16561 gactttagaa actataaaag acaggaaaaa gaaattagta ttagaaaata tagaatatag
    16621 tgagagtttt aattttgaag aatcagaaaa ctatgtttta ataatagatg aaattaatag
    16681 agggaatgta tctaaaatat ttggtgagct aataacatta ttggaagatg ataaaagact
    16741 tactaaatca aatcaaacta ttgtcaaact accatattca aaggaaaaat tttctctacc
    16801 tccaaatttg tatatagttg gaactatgaa cacatcagat aaatcaatag cacttttaga
    16861 tatagcttta agaagaaggt ttaattttga agaaattatg ccagattatg atttattgtc
    16921 tattgtagat ggtatagata ttagaaaaat gctatataca atcaataaaa ggatagagtt
    16981 tttatatgat agagaccatg ttattggaca agcttatcta cttgctgtag aaaatattaa
    17041 tgatatagta gtagtattta gaagtaaaat aataccactt cttcaagagt atttttatta
    17101 tgattcagaa aaagtaggtt tgattttagg aggaattgga agaaatgaaa aagataaata
    17161 cattgtttat aaagaaaaac ttatagcttc tgagttattt aaaaataata atgcctcaat
    17221 aatggagagt aaagttaact attgtataaa acaaaacata tctagtgagg agctaaaaaa
    17281 tatatatgag taaaaatata tatgttaagg aaacctatga atggataagg ataggtaatg
    17341 gtgaaaatga gcttacagaa attgaatatg aaaaattgct taagtatcta gagaacaata
    17401 atgatgtatt aaaaagtaat ataatagata ttaaatacaa aaaacttaga tttataaact
    17461 atgtaggcat catatgtttt gaaaatgtaa tacttgaaat acttcctaaa ttgtcattaa
    17521 gtgataatct tgtaaaagat agagagatac tattacagat gctttctata tgtaataaaa
    17581 ttccaattac aatgaatgaa aaaataagat tgagtttgaa aaattataat ctcttaaatt
    17641 tttttgttat gtatttcatt gagagtatgc aaacacagat gaaaaaaggt atttattttg
    17701 aatatataaa taaaatagag aacttaaatg ttatgagggg taaaatatta ttaagtacat
    17761 atgcaaaaga aaaaggcatt tctccaatga aaataagatg tgaatatgat gagtatagtg
    17821 aaaataattt cttgaatcaa gttcttaaaa aagcatgtat atctatttta tgtagaataa
    17881 atgacaattc aattcaaggc aagattaaaa aaattttaag ctattttcaa aatgtagatt
    17941 taatttatat agatagaaaa aaactgttgg attataaatt ttataaaaac aatgatagat
    18001 ttaaagattg ttatttacta gctagacttc tacttttaaa tttgtctatg gataatagtc
    18061 aaaataatca agaggcattt tctatattat ttgaaataaa tactttgtat gaagaatata
    18121 taggaatttt aatcaaaagt atatgggaca attcatttag agaaacatat atacaagata
    18181 agagtaaatt tcttttgaaa aatgaacaaa ctggtaagaa aaactttaat ttaagaccgg
    18241 atatagtttt atatgactta aaaaatgaat atgaaattat aattgacact aaatggaaag
    18301 ctattgaagt agattcaaat gttttttata ggtcaagtga tatataccag atgtatgcat
    18361 atataacagc atatgaaaat gcaaaaagat gtatattatt atatccttgt attcaaaaag
    18421 ataagaatta tagttcctgg aaattatcag aatcatttaa gggtaagttt
//
LOCUS       DAELTA010000077        18446 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.077, whole genome shotgun sequence.
ACCESSION   DAELTA010000077 DAELTA010000000
VERSION     DAELTA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.077"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(173..2185)
                     /locus_tag="KPG13_002164"
     CDS             complement(173..2185)
                     /locus_tag="KPG13_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985879.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2656..3306
                     /locus_tag="KPG13_002165"
     CDS             2656..3306
                     /locus_tag="KPG13_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG0985880.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(3446..4822)
                     /locus_tag="KPG13_002166"
     CDS             complement(3446..4822)
                     /locus_tag="KPG13_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0985881.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(4838..6751)
                     /locus_tag="KPG13_002167"
     CDS             complement(4838..6751)
                     /locus_tag="KPG13_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0985882.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            6887..7519
                     /locus_tag="KPG13_002168"
     CDS             6887..7519
                     /locus_tag="KPG13_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG0985883.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            7658..8197
                     /locus_tag="KPG13_002169"
     CDS             7658..8197
                     /locus_tag="KPG13_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0985884.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(8373..8543)
                     /locus_tag="KPG13_002170"
     CDS             complement(8373..8543)
                     /locus_tag="KPG13_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0985885.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            8828..9895
                     /locus_tag="KPG13_002171"
     CDS             8828..9895
                     /locus_tag="KPG13_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG0985886.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            10065..11966
                     /gene="cooS"
                     /locus_tag="KPG13_002172"
     CDS             10065..11966
                     /gene="cooS"
                     /locus_tag="KPG13_002172"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG0985887.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            11959..12432
                     /locus_tag="KPG13_002173"
     CDS             11959..12432
                     /locus_tag="KPG13_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0985888.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            12407..13633
                     /locus_tag="KPG13_002174"
     CDS             12407..13633
                     /locus_tag="KPG13_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0985889.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(13772..14959)
                     /locus_tag="KPG13_002175"
     CDS             complement(13772..14959)
                     /locus_tag="KPG13_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0985890.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            15232..16056
                     /locus_tag="KPG13_002176"
     CDS             15232..16056
                     /locus_tag="KPG13_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0985891.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            16185..17450
                     /gene="gluD"
                     /locus_tag="KPG13_002177"
     CDS             16185..17450
                     /gene="gluD"
                     /locus_tag="KPG13_002177"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG0985892.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            17663..18253
                     /locus_tag="KPG13_002178"
     CDS             17663..18253
                     /locus_tag="KPG13_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0985893.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         6893 a         2261 c         2862 g         6430 t
ORIGIN      
        1 tatgctagtt tatataaaaa aaataggaaa taaaaaaatt atcctattcc tattaattcc
       61 tattatttat gtacaaaata aattataatt tcagggtact tgaaaaataa aaaaacctat
      121 tgaaggagta ctttaatcct gaatagattt ctttgtttga taaattataa ttttatagac
      181 ctttatattt taatttattg taaaaatctt ctcctttttg agttatttta cttccatatc
      241 ttcctgaact acaaattata agttcttctt tttttagata ttcaaatatt tttttaattt
      301 ttccttctgt aatttgaagg ttcttatcga gtagtgcttt taaaattaat cctctaccaa
      361 taccaacatc tgattcctta tttaaaatta gtatctctaa tatattcaat atatcattct
      421 tactatattt tagttttaat gatgtatttt tgttatctga agaccttaag tttggaggta
      481 aatctttctc taatacaata tcttcacaca taatgtttaa ataggatgta acattttgga
      541 gttctcttat attacctttc cacatatagt tttgtataag tttttttgct tctggtgtga
      601 ttactaattt cttattaatt aaatcttcca taataattaa tatgtcttct tttctttctc
      661 ttagaggagg tatatttatt ggaattgtat ttaatcgata atataaatcc tctctaaatt
      721 ggctattatc aatcatttgt tctaaattct tatttgtagc agatatgatt ctaacatcta
      781 tattaattac attgtgagac ccaacaggca ttatctgctt ttcttgaaga actctaagta
      841 gttttgtttg taattctaaa ggcatatcgc ctatttcatc taagaatatt gttccattat
      901 tagcaagttc aaataatcct tgttttccat cttttagacc tccagtaaaa gttcccttgt
      961 catatccgaa tagctggctt tctaataaat tttcaggaac ggctgcacaa tttactgcta
     1021 taaagggttg attttttcta tttgagttgt tatgtataga ctgagctatg agttctttac
     1081 cagttccact ttcacctctg ataagtatgc ttaagtcaga tttagaaacc ttcttagcta
     1141 aatctataca ttcaaacatt ttaggactat ttgtttttat atccttaaat gtatattttg
     1201 ctatttgtcc tttttctctt aattttttag tgagattttg ttctaatttt tttatgtatg
     1261 ttatttcttg taaactataa tatgtgccca ttttttcatc tctattgtat atgttttttt
     1321 tatttacatt tatatatttc ttattgaata cgacaacttt atctagtatc tctttctcac
     1381 tcaataaaac ttttaaacta tctacaaata tatcctcaat gtattttcca tatatatcct
     1441 catttatgtc taaaatatct tttactttac tattatatgt atttatttca ccatcttttg
     1501 aagtaaataa tataccatct tttgataaat tcaatattgt atctaattct tctattttta
     1561 aaaataattc cttatattta tcttttatac cagtatctaa acttattatt tcttctgagt
     1621 atttaactag attagattgt atttcctttg aatcaatttg taataaattt attatttcta
     1681 taaatgtaga aatatctata tatctgtgac ctaaatcaaa tatatctgag ataaagctag
     1741 gtactttttc tggaactcct ggagttatag ctatttttat atttttatag ttattatcat
     1801 tcatatatgg aattggtcta atattagtca ctccaatttt ataaaataaa cttatagttt
     1861 ctagagtagt ttcatcagaa tcatttacga ttagaacatt tgtaccctgt ggtaaagata
     1921 gtagattata aattttatct tccctaaaag ttcttcttac cacaatcact tttttcttat
     1981 ctagtatatt atttataatt ttgtctaatc tttctactga cattactaat atcacatcat
     2041 catttataat atcattatca tttatttcat ttatgaagta tctattaata tttatagaat
     2101 ctccaaaaac taatttcaaa ttattttcta aaaagtctat taaatctttt ctactatcat
     2161 ttaccagagc tactgatttt ttcatttatt tctccttata taataaatag tttccaacaa
     2221 aatagttgta taaagcaata taattttgat aatttaaatt tatcaaataa aaaagaaagt
     2281 gtctataatg actttataaa agttgcatta agacactttc tttcttataa ttaaaaatct
     2341 tgtcaccaaa tccccctaat tgttgcttaa aacttaaact gtatgtatat ttttcatttc
     2401 tattcgatta aattataata tacacccaaa aaaatttatt ttaataaatt ctattgataa
     2461 tataattttg taaatataga attatattaa aataaattat atattaaaat atatatgtat
     2521 aagtttattc tgcgtcagta gaatgaattt gtcaagttta atttagttgt aattttatct
     2581 tttattaaaa ataatataaa ttaaaacata taaataaaat attttataca atatattgta
     2641 aaggaggtaa ctttgatgaa agcagaaaaa acaagtatat atccaattat taaaaataat
     2701 ttaaacaaaa tatcaattgt tgaatataaa aagggtcaaa gatttgtagc atcagataaa
     2761 gacttacaag aagtgttctt tataatagag ggagttgcat tagtggagtg tactactaga
     2821 ggaggaaata aatttcttgt tgacattgta cctgaaaatg aatttatagg caaaattagt
     2881 tacatatatg aacataattt gaaatgtgat atatttgcaa agaccaatat aaaattattt
     2941 cgatttgaaa aaaatgtatt tgaagagttt tatctcaaac cagattttat tgcactattt
     3001 cacagaaaat gtacaagaag aatatatgaa ttatataaaa ccagaatggt aagagaacta
     3061 ttttcttgca cagaagttat tgcctattgt atatcaaata aacaagaaaa taatgtctgc
     3121 aatataaaag ctattcgtaa tttcagtgaa atctattcaa ttagtagaaa aagtagatac
     3181 cattctctgc taaagttaac tgaaaaggag atagtaaaaa aaatgggtaa tacatatgaa
     3241 attttaaact atgatgaatt acataaattg gcatttgaag taaaagaatt tttggaagat
     3301 gagtaaaaat tgttacttaa tcaaaataaa gtataataat ttctgtaaaa aatagaaata
     3361 gtctcactga aaatcaaaaa tactttagtt attttcaatg agactgttta gaaatattgt
     3421 atacttataa tgtatagtat aattattaat caatttcctt tactatatta atagcccatt
     3481 ttttagataa tgctctagga acaccaaata taaacttaac cacttcttca ctataagtga
     3541 gcaaaacgag tacaaatata ggtgcacctt taaatgcagc tataaaagtt agaggaatag
     3601 aagctaacca catacatcct aattctaaaa atagagcata tttagtatct cctccacttc
     3661 ttaaaatacc aattataacc aatgtattaa aagatttaag agccattagt attcccatta
     3721 taaagaatat cttagttata tcagaagcta agctatcaga aacactaaac atttttaata
     3781 ataatggtat atttaaaatt aaaataattc caagtattaa accagctgaa aatacaagta
     3841 tagaaaactt tttagcatat tcaatggctc ttttaatatg gtcagctcca agttcatttc
     3901 caagcataat agatgcacca gatgcaatac atacagctgt cattatgaag aaattacctg
     3961 tactggttgc tatctggcta gctgctatag cagaagtacc agctcttgca tatgcaacag
     4021 aatacaggac agttccaatt gcccatacag agtcatttac aaatatagga aagctttttg
     4081 atgaaaaaga ttttataaaa attttatcaa tagcttttag attttttaga ccaaacttta
     4141 atatatagtc ttttttataa aggtaaacat aagtaatcat taaaattaat tcacatattc
     4201 tagcaataac agtagctaga gcagcacctt ttacacccaa tgcaggaaga ccgaagttac
     4261 caaatataaa tccataattt aaaataacat ttgttacaag tgcaaatgca ctacaaatca
     4321 tacctaactt aggatttcta acacctcttg aaccagtgct aaatacagta cttacagcaa
     4381 ttaatggata actaaatact gcaatactaa agtaatctat acaaagtttt accacctcag
     4441 aatcatacga aaatatgtgt ataattggct ttggtgttag taaagcagga ataacaaata
     4501 caaaacttaa aacaattgcc aatacacaag taagacctgt gacttttctt atattactca
     4561 catccttctt tccataaaac tgtgaaataa aaactccagc acctcctgtg ataccagata
     4621 aggacatatt aaaaagaaag aaaaattggt ttgcaactcc aactgaagct acagatgttt
     4681 cccccaaact acttatcata attgtatcta ttacatttac agaagtagaa attaagttct
     4741 gtatgataat aggtatacat aaagatagaa gtattttata aaatctttta ttttcgtctt
     4801 ttaaagaaaa tagttcttgc aaataatcct ctccttttta cataaattct tcccactcat
     4861 catatagaga ggcaaggtta ttttctaaat taattttttc ttggctaact tctttagctt
     4921 tatctggatt agagtatact tcttcttgac ataaaagtat atctaatttt tcaatttcaa
     4981 tctctaactt ttcaatttct ttttctatat tttgtctttt tattctattt ttcttttcta
     5041 tttctctttg ttctctctct tttctttttt catcttttat ttgagttcta gttttttctt
     5101 ctttttcttc tataatactc atctcttgga tttgcctttt tttatctata taataatcat
     5161 aatttcccag atattcagta attccatttt catctaaaac taaaatttta tcaacaacag
     5221 tatttagaaa gtatctatca tgtgatattg taaatatagt cccatcataa ttagttaatg
     5281 cctcttcaag gacttcctta gaatcaatat ctaggtgatt agtaggctca tctaataaca
     5341 ataagtttgc atttgatagt ataagtttaa gtatggcaac tctagctctt tctccaccac
     5401 tcaatgtaga aatcttctta aaaacttctt catctacaaa taaaaatgcc cctaacatat
     5461 ttctcaaagt agtttgagta agatgagtat tattttccca aatctcatca attattgtgt
     5521 tatctaaatt aagagttttt tgttcctgat ggaagtagga aacatgtaca ttagttccaa
     5581 atttaatatt gccactaaga ggttgtagtt cattagttat tattttaaat aatgtagatt
     5641 tgccaacacc atttgcacct ataagtgcaa ctttttcccc tctatatata tctaaatcca
     5701 agtctttaaa aagtattctt tctccataac ccatagatac atctctaatt tgaagaacat
     5761 cattaccact ctggacagaa ggcgtaaatt gtatacgagc ttttttccta taagcttctg
     5821 gtttatctaa aacgtctacc ttgtctaatg ctttttcttt acttcttgca cgctttaggt
     5881 gtttttctct accataagct tttagtcttt caattgattc ttcttgcttt tttatttctt
     5941 tttgttggtc ttcaaatttc ttaagctcta gttctttttc tattttagat aactcaacaa
     6001 atttagagta gttaccatta tatactttaa gttttttgtt gtgaacttca aaaattctat
     6061 ttacggattg gtctaagaaa tatctatcat gagatattaa catgacagtc cctttatatt
     6121 gttttaaaaa tacttctaac cattctattg cttcagagtc taagtggtta gtaggttcat
     6181 caagtaacaa tagagttggt ttcttaagaa gtaattttcc taaaagaact ctagtttttt
     6241 caccaccaga aagtatgctt attggcttat ccatatcatt gtcactaaat cctaatccct
     6301 ttagaactcc tttggcttca gatttatatc catatccatt taaatctgaa aataattcta
     6361 gtttatgtga atattcatcc atgatttttt ctataattgg agagtttgtt tttgtacttt
     6421 cttcagatat tttaacctcc aattctctta aataggattc catttctatc aaatttttaa
     6481 aaacttctag tacttcttcc aaaatagtat tatccgaata aaaattggta ttttgttcta
     6541 aatatccgat ttcacaatct ttagaagtgt aaatatctcc actatcatat ccataaattc
     6601 ctgaaattat tttaaataaa gtagtttttc cagtaccatt aacaccgatt ataccaattt
     6661 tatcgccttc atttactgta aaactaatat tttctaatat agaatctata ccaaaacttt
     6721 tatttaagtt attacatgac aaaacaatca taaatttcta cctccttgaa ctatctaaaa
     6781 ttataatgtt ttgatataat ataatcaagg ttatatgtat tagttttgaa aaagaaatat
     6841 tttattgtga atcaaacaaa aaagaggaca cttttaggag gtgaagatgt tgggaaataa
     6901 aaatatatca atggcagtta taagaaggct cccaaaatat catagatatc ttggagactt
     6961 attagatagg gatatacaaa gaatatcttc taaagaattg agtgatataa tagggtttac
     7021 cgcttctcaa ataagacaag atttaaacaa ctttggtgga tttggacaac aaggatatgg
     7081 ttataatgta gaagctcttc atactgagat aggtaaaatt cttgggttgg atcgaccata
     7141 caacgcagtt cttgtaggag caggtaactt aggacaagct atagccaatt atgcaggatt
     7201 tagaaaagct ggattcgaga taaaagcttt atttgatgca aatcctagaa tgataggttt
     7261 aaagataaga gagtttgaag tattagattc agatacttta gaagacttta taaaaaacaa
     7321 taatatagat attgctgtat tatgtatacc taaaaatgga gcacaagaag ttattaatag
     7381 agttgtaaaa gctggaatca aaggtgtatg gaattttgca cctttagatt tagaagttcc
     7441 gaaaggtgtt atagttgaaa atgtaaactt aacagaaagt ttatttacct tatcgtattt
     7501 aatgaaagaa ggaaagtaga caacttaaac atattaataa tgagcagttg tgaagagtat
     7561 tgagaaagtc agaagattta atattaaatc ctctggcttt ttgaaatatt aggataaaaa
     7621 tttaaataat gtatggaaat aaacaatatt aaggagagtg gtttttgtgg aggaattatg
     7681 gaaaaaggca attgaatttc atggtcatga atgtccaggg ttagctatag gatttaaagc
     7741 agcacttgca gcaagagaat atttaggatg taaacagtca ggagatgagg aaattgtatg
     7801 tatttctgaa aatgatgctt gtggaataga tggtatacaa gtcgtgctaa gttgcacaat
     7861 gggtaaagga aatttactac ttaggatgac tgggaaatca gtatatcatt tctttaatag
     7921 ggaaaatgga aaatctataa gaatatatgt aaagtcaaag ccatttcaaa tggatagaga
     7981 aggatacaaa aagtacttat tagaaagtga ttttgaagaa atatttgaga ttacagatac
     8041 taagttaaga cttcctgaaa aagcaagaat atttaaatct cttaaatgtg aagtgtgtgg
     8101 tgaatttgca tctgagcaat atataaggat tgaagaagga aagaaagtat gtcttgattg
     8161 ctttaataaa tatgaaagat tttatgagga gtattaaaat tataaattag attaaagtta
     8221 aaagtattag atatgtgcta acagtatttt aagatgaatt aggtagcaac ataataaagt
     8281 atataaataa tattaattga atcatacata attaaaaaga ggctgattag tatttataaa
     8341 ataaaatcag tctctttatg aataacgaat tattattctg gttgaggtgc atctacagga
     8401 caagctcctg cacaagaacc acagtctata caagaaccag catctataac atatgcgtcg
     8461 tctccagctg atatacagct aacagggcat tcagcctcac aagcaccaca gcttatacaa
     8521 gcgtcagtaa ttttatatgc cattttgaaa tcctcccttt tatatgtatt gtaattcaat
     8581 aacattataa caaatattat tgaatataac aattaaatta attctacaac aaaaacttta
     8641 ttatgatatt cttgttttaa agttttacaa taaagtttaa tctgcttata tttttattta
     8701 ttttaaagaa gtttatttta aaaataaaat taaatttaaa aagttatgta ttgattatat
     8761 ttaaaaatgt tatactctaa aaaagtatat tttatttaaa aatttattaa tacaaaggag
     8821 atttactatg aaaaataaat tagtttacat atgtattatg agtctattgc tatttgtagt
     8881 aggatgttct aataatccaa ataaggaaag taccattaaa aaagactcta agaataacca
     8941 aagccaagtt aaagaagaaa aaagagaacc tgtgaaaaaa gatataacta taagtttcgc
     9001 tggtgatgta actatgggga actataaagg ttcatcatat tatggcagtt ttgaccatga
     9061 atttaaaaga caaggagaag attacaatta ttttcttaaa aatgtaaaac ctgtatttga
     9121 aaaagatgac ttaacagtgg ttaatttaga aggtccactt actacagcgt caaatgcaaa
     9181 agaaaaaaaa tttgcattta aaggaaagcc tggatatgta aatattttaa aatctggaag
     9241 tgtagaggca gtaagtattg caaataatca ttcagaagat tattttgaag aaggaatgaa
     9301 agatacacag tttatattgg acgagaataa aataaattat tttggattag ggaaagatgc
     9361 tgttgtagat gtcaaaggaa ttaaagtggg tctattaggg tataatggat tatcaactga
     9421 atataatgaa aaaaatttaa aacaaatgga agatgatatt aagtccttaa aaaagaaatc
     9481 ggatgttgtt gtagtgtact ttcattgggg gatagaatta gattattatc cagataaaaa
     9541 acaaaaagat tttgctcatt atgctataga caagggagca gatttagtta tggggtctca
     9601 cccacatgtg attcaaggaa tagaaaagta taaaaataag tatatagctt atagtctagg
     9661 gaatttttgc tttggaggaa ataagaatcc ttctgataca gatagttata tatatcaaca
     9721 aacctttact ttttcagata ataaattaac ttcaattaaa gagccaaata taataccaac
     9781 ttcaataaca tctagtaact ccagaaataa ttatcaacca aaaatattgg atggaagtga
     9841 gaaggataga gttttaaata aacttgaaaa aataagcaaa gatttaaata aataaaatgt
     9901 aaaaaaaaat aaatatatat atttatttta ataatttata ttttgtttag ggtatggtaa
     9961 atcgtggtat taaataagta gattttaata gttaaattat agtcaattat aataaagcta
    10021 tttaatctac ttttttaata taaaaatata agggggaatt tgtaatggct tgtaacactt
    10081 gtaaaatgtg cgaaagtgca gacaagaaat tagaaagttt tgtagcttca aaggatgttg
    10141 aaacagcatt tcatagaaca gaagatcaga aagttaagtg tggttttgga cttcaggggg
    10201 tttgttgtag actctgttca aatggaccat gtagagtaac tcctaaatca ccaagaggta
    10261 tatgtggagc agatgcagac actatagtag caagaaactt tttaagagca gtagcatcag
    10321 gagcagcttg ttatttacat gtggtagaaa atacagctaa gaatttaaaa aatgttggaa
    10381 taactaaagg tgttgttaaa ggagaaaaaa cattaaatca attagcagag atgtttggaa
    10441 tagaaagtga tgaaaagtat gataaatgta taaaggttgc agacaaagtt atcaacgatt
    10501 tgtataaatc aagagatgat aaaatggaat tagtagaaaa aatagcttat gcacctagag
    10561 tgaaaaagtg gaaagagtta ggtataatgc ctggtggtgc aaagtcagaa gtttttgatg
    10621 caatagtaaa atcatcaaca aatttaaata gtgacccagt agacatgcta gttaattgtt
    10681 tgaacttagg tatatcaaca ggattatatg gtcttacact aactaatctt ttaaatgatg
    10741 ttatgttggg agaaccagtt ataagaatgg ctcctgtagg atttaatgta atagacccag
    10801 actacataaa tataatgata acaggccatc aacattcaac atttgcaaat ttccaagata
    10861 gattaaaaga tgaagatgtt ataaaattag ctcaatctgt tggtgcaaaa ggctttaagc
    10921 tagtaggatg tacatgtgtt ggtcaagatt tacaattaag aggagaacat tatcaagaag
    10981 tatttgctgg acatgcaggt aacaatttta caagtgaggc tgttttatca actggagcaa
    11041 tagatatagt tctttcagaa tttaactgta ctataccagg gcttgaacca atagcagata
    11101 aatataaagt aaaaatggtt tgtttagatg atgttgctaa aaaagcgaat gcagaataca
    11161 taggtttaga cagaagtaaa ttagatgaac ttagtaacac tttaatagaa aaagcattgg
    11221 agtcttataa ggagagaaga ggaagtattg aaatagatat accaaaagac catggatttg
    11281 aacaatcttt aactggtgta agtgaaaaga acctgaaagc gtttttagga gattcatgga
    11341 aacctcttat taatttaatt gcagaaggaa aaattaaagg tgttgcagct gttgtaggat
    11401 gttcaaatat gacagcagga ggacatgatg taaatactgt tgaacttact aaagaattga
    11461 ttaaaaaaga tataatagta ctatctgcag gatgttctac tggaggatta gaaaatgttg
    11521 gtcttatgtc acctggagca gaagaattag caggagaaaa cttaaaagaa gtatgtaaga
    11581 ctttaggaat accaccagta ttaaactttg gaccatgtct agctatagga agactagaaa
    11641 tagtagcaac agaattagca gctgaacttg gaattgacct accacaatta ccattagtgc
    11701 tttcagctcc acaatggttg gaagaacaag cattagctga tggagcgttt ggtcttgccc
    11761 ttggcttacc tcttcattta gctttacctc catttataac aggaggaaaa ttggtgacag
    11821 aagtattaac agaaaaatta aaagacttaa caggaggaca tgttatagtg aatccagacc
    11881 ctaaatcttc agctaatcaa ttagaagaaa taataataga tagaagaaaa aatttagggt
    11941 taaaggatgt ggaatgcaat gcatagaatt tttataaata aagatttatg tacaggatgt
    12001 aagagctgtg tactagcatg tatgctaaaa cacaataaag attatgacat gtacactctt
    12061 gatttagaaa atatagataa tgatagtaga gggcatatag aattagatag taaacataat
    12121 aatccagttc caattctttg tagacactgt gatgaacctg aatgtgtatt agcatgtatg
    12181 agtggtgcaa tgcataaaga tagtgaaagt ggaatcgttt catatgatga agagaaatgt
    12241 ggttcatgtt atatgtgtgt aatgtcttgt ccatatggat tattaaagcc agatgataga
    12301 agcaagcaga acatacttaa atgtgattta tgtaaagatg aggagtatcc tagatgtgta
    12361 gctaattgcc caagtggagc aattgaatta cagaaggagg aaaatgatga attatgtagt
    12421 gttaggggct agtgcagctg gtataaatgc agtaaaaaca ttaagagaat tagataaaga
    12481 ttcaaatata gtagtaattt ctaaagatga aaacgtatat tcaagatgta tgttgcatca
    12541 cgttatatct gaacacagaa ttttaaaaca aataaatttt gttgatgaag attttatgga
    12601 gcaaaataat gtaaaatgga ttgcaggtaa gactgttaaa ggtatagaca taaataaaaa
    12661 ggtagttcag acagaagaca ttactgtaaa ttatgataaa cttttaatag ctacaggtgc
    12721 atcttctgct attcctccta taaaaaattt aagagatgga aattttgtat attctgtgag
    12781 aaacatagat gatatttata aaataaaaga aaaagcacaa aattctaaaa atgtagttat
    12841 aataggtgct ggtttagttg gtatagatgc attagtggga ttatttaaat atgagcagtt
    12901 aaatatttct gtagctttta tggaaaagta cattttagat agacaattgg atgaatatac
    12961 agcatcagtg tatgaaaata agttcaaaga aaaaggtgta aaattttacc caagtgcatc
    13021 aatacaagaa attgtattgg ataacagtaa aaatgttaca ggtgttgcat tttcaaatgg
    13081 tgaagtttta gatgcagaca tggtaattgt tgctacaggg gttaaaccaa atgctgactt
    13141 tttagatgga acaggaatag aatatgatag aggtataata atagatgata tgtgccaaac
    13201 aacacaaaaa gacatatatg cagcaggaga tgttgtaggt aaaaatgcta tttggcctct
    13261 ggcagtaaag caaggaattg tagctgccta taatatggtt ggaaaagata agaagataga
    13321 agatgaattt gcttttaaaa acagtatgaa tttcatggat ataccaacta tttctatagg
    13381 aatgaataca cctgtagatg atagctataa agtgctaact agacatggaa ttaatgatta
    13441 taaaaagttt gtttttaaag ataatgtaat ctatggagca gttatacaag gagatatttc
    13501 atatgtaggt gtattaactt atttaataaa aaataaagtt gaaatatatg atttagaaaa
    13561 taggatattt gatatatgtt atgcagactt ttttaatata aaagaaaatg gagaattttg
    13621 ttatagtgta tagtatatac gtggagtaag gtttataaat acagtaaatc aataaataga
    13681 gttattctaa aataagaaaa atgacaaatc ttatttagaa atagctctat tttttgtaat
    13741 taaatttaaa ttatattcaa tttgtaaaat attattttga acaattatac atatactctc
    13801 tagttagagg taaatcattt tttatacctt tagtgaaaag tatttgatgt atatctataa
    13861 taccagtgtt gaaagcagca gcacaagaac aaaggtataa atcccacatt cttataaact
    13921 tatcatcaaa catttcttta atctttggaa gattctcaga gaaatttttt tgccagtata
    13981 gtaaggtttt tacataatgt aatcttagac tttctacgtc tatagtatga tatttgtaat
    14041 cactacaaat atgtatcatt tctcttaggc taggaataac tcctcctggg aaaatatatt
    14101 tttttatcca agggtctcca taagattcct gcaatccact tatatagtga agtaaaaaca
    14161 atccaccatc ttttaaaaca gcatttacgt ttttaatgaa aagttcatag ttttctctac
    14221 caacatgttc aagcatccca acgcttacaa ttctatcaaa cataaggtct gattttttta
    14281 attctctata gtccataagt cttacttcaa gatagtcttg aaggttctct tctttaattc
    14341 ttgcgttaaa ttccttaaat tgctcttcgc taagtgtaat tccaacgccg tgaattttat
    14401 atttttttgc agcttctatt agtaaaaatc cccaaccaca accaatatcg agaaggctca
    14461 taccttcctc taaatttagt ttagttaata tgtggtgaac tttattgact tgagcatcat
    14521 aaagagtatc attatcattt ctaaagtatg cacaagaata actaagtgtc ttatctaacc
    14581 ataagctata aaaatcgttg ccaatgtcat agtgggcatg tatttctttc ttttgatgtc
    14641 tcattgaatt tgatgagtgc aatagatgtt ttacagctga attatcaata ttgaatttgt
    14701 ctatttgttt taaaagtaga tttaaagtta agaataaatc tccctcaact tcaatgtctc
    14761 tatttatgta ggcttctcca agtgctaaag atgtactttt taatagttca gacttgctaa
    14821 tatcattatt tactataact ttgaactgag gggagccctc tccaattaga aattcttcct
    14881 catctttaag cctaactaga aatggtgttt caccaaattt ctctaaatag tcttttaaaa
    14941 aattttttgt aatattcata ttaccagctc cttctaatgt aaatccagta agtttccctg
    15001 cttaaaatat attatagtcc tttttttaaa aaagtaaaat tttttcaata aattttttat
    15061 ttaaaggaaa tttatttttt ataaagaaat ttcatgttgt agtatatttt ttgcttataa
    15121 attttggtac tggatataat attaaactgt taatgatata ataaatttaa tctataaagt
    15181 aaataaaatt ttgtaataaa tagttaaagc tattttataa ggagatatat gatggaagta
    15241 tatgctttgg ttgggtcaag tggcacaggg aagagcttta aggctttaga gttcgcatat
    15301 gaaaatgata tagaatatat aattgatgat gggattttaa tatacaaaaa caaggtttta
    15361 gctgggatat cggcaaaaca agcaaatact actatagaag cagttaaaag ggctattttt
    15421 tataatctgg agcatagaca agaagttaga gaagcaatta aaaaagaaaa tataagaaaa
    15481 attcttatta ttgggacatc aaaaaaaatg gttaatcaaa ttgtagagag attgtcaatt
    15541 ggaagggttt gcaagtttat aaatatacaa gatataagta ctaaatatga aatagaaata
    15601 gcaaaacaag ctagaaaaga aggaaatcat gtaattcctg ttccagcagt tgagataaaa
    15661 agtatggcaa gtggtcttag tataaattct ttgaagagac tttttagaaa aggaagtaat
    15721 aggaatatgg tagttttaga aaaaacaatt ataagaccaa cttttagtta tataggaaag
    15781 ttttatatta gtgctgatgt aattaaacag attattgaat atgaagtata caactttgga
    15841 agcattgata gaataaataa aatcaatata gagaattgca ataattttat gaatattttt
    15901 gtaagtataa atatcaatga tttagaaact ataaaaaaaa ttgaagacat acaacagaca
    15961 ataaaaaagt ctattgagaa aatgacttta gttaatgtac aaaaaataga tattaacatt
    16021 cataaaatta aaaaaataaa cttcctccaa tgttaaaaaa taaactgaga aaatgatata
    16081 ctaatttgca ataaagtgtg ctataattaa actgtaaatt taggaaatgg ttttttctaa
    16141 atggagtata cataattata acgtatttat aagggggctt tcaaatgtca ggaaaagatg
    16201 taaatgtctt cgagatggcg caatctcaag taaaaaatgc atgtgataaa ttaggtatgg
    16261 aaccagcagt ttatgaatta ttaaaagaac ctatgagagt tatagaggtt tcaattccag
    16321 taaaaatgga tgatggttct ataaaaactt ttaaaggatt tagatcacaa cataatgatg
    16381 cagtagggcc aacaaaaggt ggaataagat tccatcaaaa tgtttcaaga gacgaagtaa
    16441 aagctttatc tatatggatg actttcaagt gttctgtaac aggtatacca tatggtggag
    16501 gtaaaggtgg aataatagta gatccatcaa ctttatctca aggtgaatta gaaagattaa
    16561 gtagaggata catagatgga atatataaat taataggtga gaaagttgac gttcctgcac
    16621 cagatgtaaa tactaatggt caaataatgt cttggatggt tgatgagtac aataaattaa
    16681 ctggacaaag ttctataggt gttataactg gtaagccagt tgaatttggt ggttctttag
    16741 gaagaacagc tgctactgga tttggtgttg ctgttactgc aagagaagct gctgctaaat
    16801 taggaataga tatgaaaaaa gctaaaatag ctgttcaagg tataggaaac gttggttctt
    16861 atacagttct taactgtgaa aaacttggtg gtactgttgt agctatggct gaatggtgta
    16921 aatcagaagg ttcttatgct atatacaatg aaaatggttt agatggtcaa gctatgttag
    16981 attatatgaa agaacatggt aacttattaa acttcccagg agctaagaga atatctttag
    17041 aagagttctg ggcttcagat gttgatatag taataccagc tgcattagaa aactctataa
    17101 ctaaagaagt tgctgaatct ataaaagcta aattagtttg tgaggctgct aatggaccaa
    17161 ctactccaga ggctgatgaa gtatttgctg aaagaggaat agttcttact ccagatatat
    17221 taactaatgc tggtggagtt acagtttctt acttcgagtg ggtacaaaac ttatatggat
    17281 actactggtc agaagaagaa gtagagcaaa aagaagaaat agctatggtt aaagcatttg
    17341 aatctatttg gaaaatcaaa gaagagtaca atgttacaat gagagaagct gcttacatgc
    17401 attcaattaa gaaagttgct gaagctatga aattaagagg atggtactaa gatttactct
    17461 taataaataa taggatataa ttatttaaga tataaagggg ctgtacatag tacagccctt
    17521 aaattatgtg gcttacaatg aagtaagcta tttttgtttt aacttataaa aaacttgata
    17581 aattatgtaa tttagaatgt attttttacc accacatgat tacaaattag aagagttaga
    17641 ttagattttt agacatgaac ttatgcattt taaaagcaaa gaccttaatt taaagtatct
    17701 agtattattt ttaaaaatag tatattggtt taatccattt atatatataa tggaaaaata
    17761 tatggatttg gattgtgaac tttattgtga tgagagagtt ttaaaaaatt gtactgtaca
    17821 acaaaagcaa gaatatgctc taactattgt aaattcaatg aaaagaagta caaatttttc
    17881 aaataaattt gtagctggat taaataaaca atctgatatt agaaaaagag taataaacat
    17941 gttcagtgaa aaacataaaa atggtatctt aagggctttg attataagct tgttttcttc
    18001 tattacattt tttaaattcg attcaactag tattattgaa gagaatgatt tcttagagag
    18061 gtcaagatat acgtttgaag gaaggcaaga agacatgttt gtaacaatag attttatata
    18121 tgcagatgta cctataaaac ttagaaaaga gcatgaagaa aactgtagag tcaatggaat
    18181 agtaccaaaa gattcagatt caatagaaat tattcctaga ttttataaag attgtatgga
    18241 tttaaaaaaa taagttagac ttaatattga tgttgatatt atgaattttt tatatttaag
    18301 caattgaaaa ttttattata gattcaattt tatatgtgtt taagtatgaa atttatagat
    18361 agttgaaata attaaatatg attgatgcat aggaatcatt actaagctat aattaattat
    18421 aagggagttt aaataaatta tgagtt
//
LOCUS       DAELTA010000078        18277 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.078, whole genome shotgun sequence.
ACCESSION   DAELTA010000078 DAELTA010000000
VERSION     DAELTA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.078"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(391..813)
                     /locus_tag="KPG13_002179"
     CDS             complement(391..813)
                     /locus_tag="KPG13_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG0985894.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1291..4194
                     /locus_tag="KPG13_002180"
     CDS             1291..4194
                     /locus_tag="KPG13_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0985895.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4227..4673
                     /locus_tag="KPG13_002181"
     CDS             4227..4673
                     /locus_tag="KPG13_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0985896.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4796..5098
                     /locus_tag="KPG13_002182"
     CDS             4796..5098
                     /locus_tag="KPG13_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0985897.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5279..6685
                     /locus_tag="KPG13_002183"
     CDS             5279..6685
                     /locus_tag="KPG13_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0985898.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7029..8087
                     /locus_tag="KPG13_002184"
     CDS             7029..8087
                     /locus_tag="KPG13_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0985899.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8186..8872
                     /gene="araD"
                     /locus_tag="KPG13_002185"
     CDS             8186..8872
                     /gene="araD"
                     /locus_tag="KPG13_002185"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG0985900.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8890..9537
                     /locus_tag="KPG13_002186"
     CDS             8890..9537
                     /locus_tag="KPG13_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG0985901.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(9710..11077)
                     /locus_tag="KPG13_002187"
     CDS             complement(9710..11077)
                     /locus_tag="KPG13_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG0985902.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11267..11355)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11783..13903
                     /locus_tag="KPG13_002188"
     CDS             11783..13903
                     /locus_tag="KPG13_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG0985903.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14007..15260
                     /locus_tag="KPG13_002189"
     CDS             14007..15260
                     /locus_tag="KPG13_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG0985904.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(15414..16475)
                     /gene="ytvI"
                     /locus_tag="KPG13_002190"
     CDS             complement(15414..16475)
                     /gene="ytvI"
                     /locus_tag="KPG13_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0985905.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            16749..17489
                     /locus_tag="KPG13_002191"
     CDS             16749..17489
                     /locus_tag="KPG13_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG0985906.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            17501..>18277
                     /locus_tag="KPG13_002192"
     CDS             17501..>18277
                     /locus_tag="KPG13_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0985907.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTF"
BASE COUNT         7317 a         1985 c         3063 g         5912 t
ORIGIN      
        1 ttgagagttt tatggaagag gggaatatat tttctattag agataagaat atgacatata
       61 ttttttattg atttttacaa aaatactata tagtgaataa attaatatct tttctaaatt
      121 agaagaggag aatcttatta gataaagatt atcctcttta ttgaaagttt aagggatatt
      181 agagattatt aatttttctc tcttttttta actaaaagca ataatacata aaattattat
      241 tgctaatgat actcttaatg tggtatatag tcatagtgtc acatgtttat caatagtttt
      301 ttcattaatt attgtaacga atagtaggac tcataaaata cttagaaata tgtttttaga
      361 tagattttaa atatataatc taacttatat ctataaatta acactattat tctccaattc
      421 catttctagt ttttttataa atttattaaa ttcttctggt tgcctttttc caaacaatac
      481 tcttacaaga gctgaattga tttttccaaa tatattcctc ttgctctcac tttcaataaa
      541 aataagtttt gtattttcta agtcaatatg tactaattca tatctagtaa tagtactttc
      601 tcttttattg cttgttttta tctcatatac tctatttttt tcgaaattag ttatttcaac
      661 cttaaatctt ttattatttt tttgctgtac aaatgttcct actgcttttt tctcattaaa
      721 attaggaaaa tttagtcttg cagattttgt aaatatatta aatatgtctt ttgcagaata
      781 ttttaataaa acttcagttt taaatgtatt catatcgttt tcccttctgt atataaattt
      841 aatatttata agttaatttt taagttaaaa tagtataata tgccatatta aatcatgtta
      901 aaactattta tatagctaaa atcttaccta tataagtatg tatactatca tataaaaata
      961 aaatcttaaa atagaaaggg ttaaaaacac agtttattat aatttttatt gttgagtaga
     1021 tacgtactat tatatatgat tgagcaagtt tttcaatcat aaatttataa agtattttgc
     1081 attaaaattt aaacaaagaa ttaaattatt taagattatg aataactaat acatttagta
     1141 aattactaaa tatacaaaat gtaaatactt atcaaaatgt ataaatactt atgacatcta
     1201 gttacttaca gggtttaagt agaaaaaaat atcaaagtaa gtatggcatg atagttgctt
     1261 atatatttat caagaaagga gttattaagc atggagttga gagataaact tgtaaacatc
     1321 ataaatacag aaaataaaaa agatccatta acagatgttc aaatagcaaa gtttttaagt
     1381 acaactagag agaacataac taatttgaga aaagaactta atataagtaa ttcaaggcaa
     1441 agaagatatc cttatttaaa atcagcaatt tcggcaatat tacaaaagaa taagaatatt
     1501 tctatatcag aaattacaag ggaattgatg acagaaggct ttaatatatc gagaagagtt
     1561 gtggaggaac ttttacctaa agaaagttta gaaatggatg tagtagagga agatgaagaa
     1621 gaatcgaatg acccatttga aactctgata ggtaataaag ggagtcttag aaatgctgta
     1681 gagcaggcta aatctgctat attgtatcca ccaaaagggt taccaacgtt gattattggg
     1741 gaaagtggag ttggtaagag tttattttct agacatatgt atgagtttgc aaagcaaaaa
     1801 aagatagtaa aagagagctc caattttgtt gtatttaact gtgctgatta tagtgacaat
     1861 ccacaattat tattatcttt gttatttgga tataaaaaag gtgcatttac aggtgctgaa
     1921 tatgatacac caggtcttgt agaagaagct gatgaaggag ttttattttt ggatgaaata
     1981 catagattgc ctccaaaagg tcaagaaata ctattttcta tcttagatag gggaaagttt
     2041 aggagattag gtgaaactaa tgctgaaaga aaagtaagta ttatgttgat tggggcaact
     2101 actgaaaatg tagagactaa tttattatta acattcagaa gaaggatacc aatgctaata
     2161 actttgccac cattacatga tagattacta aaagagaaaa tagaccttat atacaatatc
     2221 tttcaacaag agtgtaatag aataaatgca aaaatatttg tagataaaaa tgtgattgaa
     2281 atacttgcac taaaaaagtt tagtggaaat ataggacaat tacaaaatat gattcaggtt
     2341 ctttgtgcaa gagcttttat gaagtttata aattccaaag atgaagatag tgaatctata
     2401 gtagtagtag atataaatga agttttaaag cttaaagatt cgtttaaaga tgctgccttt
     2461 caagagattg aatatacaga gataagaaaa tatttgaaaa atgctatatt tataccattt
     2521 aatttagaag aatcatcagt aaaaggaagt cttttaagtg atgaatatat attaccagag
     2581 gacatataca aaacaattga aaagaaatat tatgacctaa aatccctaga cattgaagat
     2641 atagatatag agaatatttt atggactttt attttaaata gatttgctga cttaaagttt
     2701 aatcttgata gtaatgaaga aattttttca atgagtgatt taaatagttt tgtaagtgat
     2761 agtttagtta aactggtaaa agaacttatg aatgatatag ttgaaaaaaa tatgaattta
     2821 gaagtaaata aaaatatgtt caaatatcta gcaatacact tagaagaagc tataaaaaga
     2881 attaagctaa atcaaaagat aataaatgta aatttagaaa aaataaagat tgacttttca
     2941 aaagaatatg aaattagtaa aagttttgca ataaaaattg aggaatcaat tggtattaag
     3001 atttcagatg atgaaattgg atttataacg ctctacataa agtctgctct aaaaaatgaa
     3061 gtgaaaaaag ataaagtagg gttaataata atttctcatg gacgtatagc tacagagact
     3121 gtaaatgtag ttaaagaact tttaggtgta aagtttcctg tggctataga tatgccttta
     3181 gatgaaaaac caataaatat atacaataag gcagtagaat tgtcaaaaat aatagaccaa
     3241 gggaaaggca tattgttttt ggtggatatt ggttcactta caaacatagg tcaaatagta
     3301 aataagagaa ccaatataag tactaaaact attgatagag tagacctcct aatggcacta
     3361 gaagcaacta gaaaagtttc tatgggagag gaagaacttg atgaaatatt tttttctttg
     3421 ctaaaagata ggatgggata taactatgaa ttagaaaaac acatagataa atcaaatgca
     3481 attataacat tatgtcttac tggagaagga actgctaagt atataagtaa aacgttggaa
     3541 gaaaagtatg ataacactaa atgttatcaa atgagtgcat tggatgaaag tttattttgt
     3601 aaaattgaaa atctaaaaga gacaaacaat atattagcta ttgttgggac aataaaccct
     3661 aagataccag gtataaattt tataccctat aataaagaac ttttcaaaaa tttggatatc
     3721 tatctatcaa aatcaaaaca acaagatgac agaccaatta aatcgtatga aagaatgctt
     3781 gatgaggatt tagtgatttt tgaaccagat atatatttta aaaaggattt attagaatat
     3841 gtatgttcaa ttcttataaa taaagaatat gtagaaaaag attacttaga ttctgtttta
     3901 catagagaag aaatgcttcc aacatattct aaaggagcta taggtgtgcc tcatggagat
     3961 tcttctactg taaatagtac aaggtttgtt tttgttaaac taaagaatcc tattgattgg
     4021 ggtgtaggta atgtaaattt catatttatg ccagtttttc aggctaatga taaagagatt
     4081 gttaaaaatg ttcttgagat actaaaagat gcagaattta tgaacaatgc caataagtgt
     4141 tttgataaag atgcttttag aaggattata tttgataaat ttaaacatct gtagccatag
     4201 caaaaaaatt gagaggagtt agagtaatga atgtagattt aatatcattg ggtttagaat
     4261 ctaatgatta tgaaagtgtt attgaagaat taggaagtat aatgtgtaaa aaagaatatg
     4321 taaaagaaac atacataaat gctgtcttgg aaagagaaag gacattacca acaggtctag
     4381 atattggaga catgtgtgtt gctattccac atacagattc aaagcatgta aatgagtcta
     4441 atgtagctgt agggatattg aaaaatcctg taaagtttaa ttcaatgata gacccaaaag
     4501 atagacttga tgtagaactt gtatttttat tagcagtaaa gaacccagac tcacaagtaa
     4561 aactgttaaa agatttaatg tcagtttttc aaaatataaa gttattaaaa aatataaaaa
     4621 acgcatcaac aaaagaagaa gtagctaaat tattggattt tatagaaatt taatgtagga
     4681 gaattttata aataaaaaat taaaatttaa acaatcaaag taagtattat ttttttaata
     4741 ggtttaatct tatataaaat aaaaatttag agtgaaaaat taggaggaaa ttattatgaa
     4801 acaatttaat gtattatcag tttgtggttc agggacagtt acatcatcta tggtagcagg
     4861 taaattaaaa gaagtactag gtaataaggg gatttctatt actacaacag aagctagacc
     4921 aacagaagca ctaaatttag cacaatcagg aagatttgac tttataacat ttacgagtcc
     4981 acttcaaaca ggcgattatg gaataccaac tataaatgca tttgcttgcc taactggtat
     5041 aggggaagag gcattctttg aagaagtttt gactgttatt gaagggatag ataagtaagt
     5101 tttggagacg tgaatttata gtatttaaat caatgattta gatactataa taagctaata
     5161 ttaactaaat atttgtggaa aaaacatttt atatgttgtc ataaattaaa aaattataaa
     5221 aatagtcact taagtattaa attaagctta taaatattta taagtaggag ggaaaaagat
     5281 gttagaattt atgaaatcta taatcgatac atttggttct gcaattatag ttccaattat
     5341 aatattcata atcgcaaaga tatttaaagt aactactaag aaatcgtttc tttctgctgt
     5401 gtatgcagga gttgctttac aaggttttac actgatttta aattcattta caccagtaat
     5461 aacaccagtt atcaatagga tggttgagag tacaggagta aacctaccag tttttgatgt
     5521 tggttggcaa gcaacatcat tggttgcatt ttcaacaagt gcaggtatga tatatttagg
     5581 tcttggtata ttattacaaa caattttatt tttgataaaa tggacagatg tgtttcaacc
     5641 atcagattta tggaataact attcttatat ggtatgggga gcaatggtta taggagtaac
     5701 aggaaatttt cctttaggta tagcatgtat ggttttatta aacctatata gtttattaat
     5761 atcagagtta gttgcaaaga gatggtctag ttactataga tatcctaatt gtacaataat
     5821 agctatgcat aatgttgagg cttctgtgtt tgcagtattt gcagacccta tatacaataa
     5881 attgggatta aataaaataa aattaaatcc aaaagaatta gagaagaaat tagggttttt
     5941 aggtgaacca attactttag gtcttttttt aggtatgttt ataggtatat taggtaatat
     6001 gacgagaata aatactatgg aagcttgggg agaaataatg aaagtaggta tatctacaag
     6061 tgcagtgatg gcaattttcc ctaaagtagc aagtatgttt gctcaagcat ttgcacctat
     6121 aacagaagca gctagaaaga ttatgcaaaa agctggaaac cgtgagtggt acatagcagt
     6181 aaatgatgct gttggatatg gtgaacctgc aactttaata tcaggtctta tattaattcc
     6241 aataatgctt gtaatagcta tggtacttcc tggtaataag gtattaccag ttgtggattt
     6301 attggcaatt ccatatatgg ttcaaggttt agttgccata cataatggta atataccaaa
     6361 agtattagtt tcaggtataa tttggtttgg attaggatta tatgtttgta catctacagc
     6421 accattattt acagatatgg ctacaaatat aggtgtagct atacctgcag gagcaatgtt
     6481 aataacaagt tttaatatac taggaaaacc tttgatggga ttagttttct ttgcatttct
     6541 aagtgcaaat ccaatttata taggtctttc agttgtaatt tactttgttc tatgggcatt
     6601 gtttagaaag aacaaaacaa gtattcttga ttatttagaa aaacaagcac ttaaaaatgt
     6661 agaagaagag cctgtagcag tttaagttta taaaaatgaa ataaaaataa ttaaaggagt
     6721 ttataattga ttttatataa gtatcagata atacctgaga aatatttaaa tgaaatatag
     6781 acttcttttt atttgataat atttgtaatt ataatgcatt gtatttttat agttgagttt
     6841 tctgtataac ctgctattta aaacatactc ttttatattt atacagtaat gtggtttaat
     6901 tttataaaat gtaatctcta ctggtaaaag actttgtata taaggcattt gcctgaataa
     6961 accatttatc ttttgtatgg catagatatt gctatataga agtatatagc atgaaaggag
     7021 atatttatat gggaaataaa atgagagcat cagtattgta taatgtaggt gatgtcagat
     7081 atgaaatggt aaatatacca gaaataacag atacgcaagt gcttgtaaat gtaaaatatg
     7141 ttgggatatg tggttcagat ttacctaggt ctatggtaag tggtttgagt ggaaatacaa
     7201 aatatccatt gattttaggg catgaatttt ctggtgaagt tgttaaaatt ggagaaaaag
     7261 taaagcatat taatgtagga gatagagtag ctgttgcacc attagtacct tgtggaaaat
     7321 gtgattattg taatgaaggt aattttggct tgtgtgatga ttacaatata attggaacaa
     7381 gagtaaatgg agcgtttgca gaatatgtaa gagttccaga agaacatatt ttaaaactac
     7441 cagatacatt ggattatgaa actgctgccg gaattgaacc agcaactata gcttatcatg
     7501 ggatttcaaa gtctaatatt agagtaggag atagtgtagt tgtgttagga tgtggtccaa
     7561 taggtcagtt tgttatacaa tgggcaaagg tgtttggtgc atctaagatt atagcagttg
     7621 atatttttga tgaaaaatta gaactttcta aacttctagg agcaaactat atacttaact
     7681 caaaagaagt aaatgtaatc aaagaaataa aaaaaataac aaatggtggt gctgatgttg
     7741 taatagaaac tgctggtagc cgatttacac aagaacaatc tctatttgta gcaaaaaaga
     7801 gaggaaatat agtgtttgta ggtatatcac acacagaatt accacttagt gcagacgcta
     7861 ctgagtgtat attacgtgga gaacttacct tgaaaggttc ttggaattca tatacaagcc
     7921 cttatcctgg aagagcatgg actgcaacat tggattttat ggaaaaaggt gacattattt
     7981 ttaaacccat gatttcagat aaaataggtc ttaatgaagt gggtgacttt ttaagtaaaa
     8041 tgagcaagag agaaattaat ttcaataaaa tcctagtaga aatataaaaa tttaataaaa
     8101 aattctaaaa gcaattaaat tttataacta tgacattaaa taaaataaaa ataattttct
     8161 taaataaaat ataggaggaa gaaaagtgtt agaaaattta aaagaagaag tattaaaagc
     8221 aaacttagaa ttacctaaga aaaagctaat tacttatact tggggaaatg taagtgggat
     8281 agatagagaa aaaggattag tggtgataaa gccaagtggt gtagagtata gtaatatgac
     8341 tgtagaagat atggttgtag ttgatttaga gggaaatata gttgatggca aattaagacc
     8401 ttcttcagat accccgactc atctagtttt atataaagag tttgaagaat tggggggaat
     8461 agtacataca cattcaagtt gggcaacaat ttgggctcaa ataggaaaat ctataccatc
     8521 ttgtggtact actcatgcag attattttta tggaagtata ccatgtacca gaaaaatgac
     8581 tagagaagaa atttgcaatg aatacgaaaa ggaaactgga aatgtaatca ttgaagaatt
     8641 tatgggaaga aatccaattc attgtccagg aataattgta aatgaccacg gaccttttac
     8701 atggggaaaa gatgcaaatg aggctgtaca caatgcagta gttttagaag aagtcgcaaa
     8761 gatggcttat tatacagagt taatgagtcc tgataatatt atggataagg ttcttatgaa
     8821 taagcatttt tcaagaaaac atggaaaaaa tgcatactat ggtcaaaaat gattatctgg
     8881 aggtgaatta tgctagactc tttaaaaggt aaagttgtgg aaatagcaat taaagcagag
     8941 cattatggtt tatgtaaaga aaagactggt aattttagta ttagagataa ggatactgga
     9001 tatatactta taaccccatc aggtgtagga agagaagaat taaaagttga acatatttgt
     9061 gtattggatt tggatggaaa tgtaatagaa gttgagaaag gtattaagcc aagtagtgag
     9121 gtattgatgc atattgaaat atacaaaaca agagaaaata taaatgccat atcacatact
     9181 cattctttat atgcaacagc attttcagta gcaaagaaac caattatacc aattgtatat
     9241 gaatcagtta attatggcgg ttatgtagat attgcttcat acgaaagacc aggaacttta
     9301 aaattagcta agagtgtttc aaaattactg atagatacag atgcatgctt attagaaaga
     9361 catgggttga caactgtagg taatgattta gacgaagcat tattaaaatc aagatatgtg
     9421 gaagaggtag cagaaatata ctataggtct ttagtattaa atcaatttaa agaacttgaa
     9481 gtagtagatg ttgatgagtt taaatcttgg aaatacccag aagaaataaa gttttaatct
     9541 aaataatttt cattgaagtg gttaattaaa atatatgtat tatttatatc aattactatg
     9601 ttttattatg ttaattaccg tattttgcaa atatatttat tacatttttt caaaaaaata
     9661 tagaatccga aagccatata aaattgactt gggattctat actttttatt tatgcatttt
     9721 tctttatctg attttctgga gaatttatat gttcaaaata ttttttagtt tcagatacta
     9781 ttataggtga taatgcaagt aaagctataa ggttaggtat tgccataagc ccatttacaa
     9841 tatcagcaat tatccaaaca acttctaact ttaagaaagc acctaataac accatagcca
     9901 caaatacagt tctataccca ttcatgcctt ttacaccaaa cagaaattca aaacatcttt
     9961 ctccataata actccatccc aaaattgtag taaatgcaaa taatgttaaa ctaactgtaa
    10021 gtaatattgg tccaaatgta ggaattgctc cgttaaaagc tgcttgtgtc ataacagctc
    10081 catttaggtc tgaacaccac actcctgaaa taacaagaga gaatccagta agagtacaga
    10141 ttataatagt atctataaaa gtacctgtca tagatataag accttgttcc gctggccatt
    10201 cagttttagc agcagctgca gctattggag cactacctag accagactca tttgaaaata
    10261 caccacgagc gataccattt ctaattgcca gtgacatagt tgctcctaaa aaaccaccag
    10321 tagcagcagt aggtgtaaat gcacttttaa ttattaacat aaaagtttca gggattttat
    10381 tccaaaaaga tattaaaatg attccacaga ttacaacata tataactgcc ataaaaggta
    10441 ctattttagt agctacatta gatatacttt taagtcctcc aaatacaact aaagctacaa
    10501 atactgctaa tacaacccct gtaattctag ggtctatccc gaaactatta tttattgcat
    10561 ctgttattga atttacttga gtaaaagtac cgattcctaa aagtgcaact agtataccac
    10621 ttatagcgaa aaatattgca agaggtctcc atttttctcc cataccattt actatgtaat
    10681 acattggccc accagacact tgaccatttt tatctttagt tctgtatttt attgcaagaa
    10741 caccttctga atacttagta gccattccaa agaatgctgc tatccacatc caaaaaagtg
    10801 caccaggtcc accagctttg atagctgtag caacaccaac aatattacca gtaccaactg
    10861 tagcagctag agcagtacat aaggctgcaa aacttgaaac atcaccacta cctttgtttt
    10921 cagcagagaa tattaactta agagctcttg gcagtttagt tatttgaaca actccaagct
    10981 taacagtaag tagtatacca gtaccaacta atagtaccag taggggtggt ccccaaataa
    11041 aagcatcaac tttgtttaaa atatcaatta gattcataaa aacactccct catttttttt
    11101 gctttacata atcaatagaa tttttaatga agtaattgac aggaaataag tataatttat
    11161 taataatttc tagtgtaata aaaaaagcta aatctaaaaa agattcagct ctggatgtac
    11221 actaattagc aaccaaaata aaagtattag taaatactgt ttttggtaga taatccctgt
    11281 ccttttgcct gagagattga gcataaaagc cttgcccctt cggcgcccat aatgggtctc
    11341 tccagagttc cgtaaatttt acagtcctac ttttaaaagt acctgagagt gttactcctt
    11401 cggtgtgcta tatagcaact cttctataaa atcttaaccg gatattgatt tgtcatagct
    11461 tattgtatta ataatattaa tataagtttt tatgttttgt caactaaaaa attaaaaaac
    11521 aaagaaaaaa tataaaaatg taagcctatt tacgaattat aaacttagaa agatgagtaa
    11581 tatacgtgaa aatgaatttt gtaatacaca tacaaatgaa gtgtaaaata agttttaaat
    11641 aaaaaaataa taaaatctta aataattaat tagatatgat taaattatgg taatattata
    11701 gttacgatat taagaaaagt tctatttagt tagattttaa gcgtaatatg agattatgta
    11761 tagagaaagg atgtattttt agatgggaaa aatattagtg ctagcagaaa aaccttctgt
    11821 tggaagagat atagcaaaag ttttagggtc aaaaaatgaa aaaaatggtt atatagaggg
    11881 gccaaagtat gtgataactt gggcactagg tcatttggtt acacttgctg accccgaaag
    11941 ttatggagaa agatataaat catggagctt agaagacctg ccaatacttc ccaagcacct
    12001 taagactgtt gtaataaaaa agagtggaaa gcagtttaat acagttaaat cgcaaatgaa
    12061 tagaaatgat atagatgaaa tagttatagc aacagatgca ggtcgtgaag gagaacttgt
    12121 agcaaggtgg ataatagaaa aatcacaggt aaaaaaacct ataaaacgtc tttggatttc
    12181 ttcaagtaca gacaaagcaa taaaagacgg atttgcaaaa ttaagaagtg gaaaagaata
    12241 tgaaaatttg tattactcag caatagcacg agcagaagct gattggatta ttggaataaa
    12301 tgcaaccaga gctttgacaa caaagtacaa tgcacagctt tcttgtggaa gagtacaaac
    12361 tcctactctt gcaatgttgc ttaaaagaga agaagaaata agaaatttca aacctaaaga
    12421 gtattatgga ttagaattaa ttgctacaaa gggaaattca gatataaaat ttatttggaa
    12481 tgataaaaac aataattctt caacttttag taaagaaaag atagaatcca cattaaaaaa
    12541 agtaaaaggt gtagacataa aaatatctga agtaaacaag tcatataaga aaaaatattc
    12601 acctgcactc tatgatttaa cagaattaca aagagatgca aataaaatgt ttggattttc
    12661 agcaaaagaa actttatcta taatgcaaaa actgtatgaa catcataaag tacttactta
    12721 tcctagaact gattctagat atttaacatc agatattgtt ggaacattaa aagatagaat
    12781 aaaagctgta aatattagtg attattcaaa gatttgtaat agattattaa aaagcaatat
    12841 tatagctaat aaatcgtttg ttgacaattc aaaggtaaca gaccaccatg caataatacc
    12901 aacagaacag aaggtattca tgagtgattt tagtgataaa gagcgaaaaa tatataatct
    12961 tgtagtaaaa agatttttag cagtactatc tcctcctttt gaatatgagc agacaactat
    13021 aagtgcaact gtagaaggag aaatatttaa ggctaaggga aacaaagtaa taaatttagg
    13081 ttggaaagaa agttataaag atattgaaga tgaagatgag tatgaaagta taccaaatgt
    13141 tttagaaaaa gatgtactta aggtcaaggg attaaagata acttcaggaa aaacaaatcc
    13201 accaccatac ttaaatgagg gaacattatt gactgctatg gaaaagaatg gtctagggac
    13261 agttgctaca agagcagata taatagaaaa attattcaat tcatttttaa tggaaaagaa
    13321 aaataaagat atatatataa catcaaaagg aagacaatta ttagatttag ttccagtaga
    13381 cttaaagaca ccagaactta cagcttcatg ggaaaagaaa ttattggata tttcaaaggg
    13441 aaaacttagt aaaaatacat tcataaatga catgaaagat tattcaaaag atattgtact
    13501 agaagtaaaa aatagtgata ataaatttaa acatgataac ttaactaaaa atagatgtcc
    13561 agaatgtggt aaatttatgt tagaggtaaa tggtaaaaaa ggtaagatgt tgatttgtga
    13621 agatagagag tgtaatacta gaaaaactat atcacaagta accaattcaa gatgtcctaa
    13681 ctgccataaa aaattagaac ttcgtggaga aggtgagggg agactcttca cttgtagttg
    13741 cggatacaga gaaaagttgt cttctttcaa caagagaaag caagaagaaa aacagaaggc
    13801 atcaaaaaaa gatgttaatc aatatttaaa aaatcaaaat aaaaatcaag agacatttaa
    13861 taatccattt gcagaagcat tagctaagct aaaaaacaaa taattttaaa gttattatta
    13921 tacacaatta taactttaac tgtatattag ttatatgtac tgatataata tagtgtatag
    13981 tttaggcatg aaagtagggc tgttaaatgg atttaagtgg catatttaaa tactattgta
    14041 aagagtgtga aaatacatgg aataattcga gtgttgaatt atttgagaat atagaaacgt
    14101 atagtaaaga ttcacaaaaa aagagggaaa aagaattaga taaattgcta aatacaatat
    14161 cagttcattt agagaggtat ccaagtgatg ctgtattgag aaaaatgtgg gtaaaaaagg
    14221 gcgaggtttt cttacaaaag acattggaaa aagaaaatat ttttaagtta gaaaaaatgg
    14281 atgtagagga tagaaaaaaa tttttagata taacaaaaca gtttattaga gatgctagaa
    14341 aatttgatga tgatttacct ataggtgata ttatgcaagc tatgaggaat gtatggattt
    14401 caaatgcatt acaattatta tttggtaaag aagtatatta ttcaaaagct aactttgcat
    14461 atagtatgtt atatccatat actgataatt atttagacaa tacaaatata gataaaaatg
    14521 ataagatttt atttaataac tggttagaaa aaaggctcct gggagaacac attaaatcta
    14581 aggattatca tgaaagtaaa gtatctcaaa tgatagatta tattgaaagt gtatacccta
    14641 gagaaaagtt tacagaagtt tatgaatcgt tattattaat atttaaaagt caagtaaata
    14701 gtttaaaaca acatggtaag gaaaatcatt tgtgtaaaga agatttatta tccatttcta
    14761 ttgaaaaagg aggttcatcc gttttagtag atggatattt aataagtgga ttgatgacaa
    14821 aggaagaaat agagttttgt ataggatatg gatttttatt acaaatatct gatgatttac
    14881 aagatataaa agaagattta aaatacaacc ataaaactat tattacagag atgtcaaaag
    14941 agggtacttt agataaagtt gtaaataaac taataaattt tactattgag ttaatagata
    15001 gttttaaaat taataataaa aataaatctg taatagctat gataaagaat gattgcttaa
    15061 tgttaatttt attttctgta gtttataatg ctgaattttt ttctgtagga tatataaaag
    15121 aagtagagaa atttattcca tatacaatag attattcatt agagattgaa gaaaaaataa
    15181 aagaaaaatt taaaaatata gatgttttaa ataatgaaaa tgaatataaa gaaatgattg
    15241 atattatttg tgcagagtag aaaaaaaggg agtgtcttag aatagacttt ttagtttatg
    15301 aggcactctt tttgtataaa atcaaatgta ttttcatcat tttaacaatt aaaaagagct
    15361 tatccctatt tgagaaagcc ctttttaatt taaataaaaa tgtcaaggtt aaactattta
    15421 aatgcagtaa taatcccatt atcatgtaag ttttttatga ttgtagctgc gatagggaat
    15481 ccaaataatc cagctattcc aaatagttga gctccaacaa acatacacat taatgttatt
    15541 aaaggatata ggccaatttg gtgtcctact acctttggtt ctaatatttg tctaacaacg
    15601 gttataatta tgtatagtat taaaagtcct attgctaaat taatatttcc attaatgagt
    15661 gagataatca tccaaggtgt caaaatactt cctgtaccca agactggcaa aatatcaaca
    15721 agtgctatga tgacagatat agtaaatgca ttttcaacct ttaggatagt aagtccaata
    15781 agtagttcta tgaatgtcac tgatagtagt attgcatagg cttttagtag ttttaaaaga
    15841 gcattaatac tgctattttt tatatttatt atcataaatt gacttttttc tggaaattgt
    15901 tttaagataa agtttactat tttttgatag tcaaaagtaa agaaaaaaga agcaattaaa
    15961 gtaataataa gttttaacat aaatgcaggt gttttacttg caatactagc aatagcattg
    16021 agtgcatttg ttgaaataga agctactaaa gacatcatag attgacttat attatcccaa
    16081 ccaagtatta atgctaggtt aacattaggt gtactagcat ctatctttga aaataaagta
    16141 tttagcgttg gttgtatact tgatttatat atttcaggaa gtctaaaaaa tacatcactt
    16201 acacttgcaa atatcttcac accaaagcta actaataaaa atatacacaa cccataaaaa
    16261 attaaaagta ttactataga aataagtgta cgatttaaat ttgtattatt tgtaataagt
    16321 cttataatag gtcttaagat ggatgcaatg ataaaactga ttacaaaagg cattaataat
    16381 ggcaataaga attttaatac aatatataca atacttgtta ttaaaactac atatgtaaat
    16441 ttaattataa aattcttttg cttttgaata tccataatat aaaacctcca atgtatttga
    16501 tattattcta aatatggtct tttttacaga aaatatacta ctgtatattg attataaact
    16561 ataaagctta tgtaaagtaa ataaattatg taaaatttag gttaattttg tataaagaga
    16621 tacttttata ttttacaatt aataaccaaa tactaaaaaa tattttaaat aaagaactta
    16681 aaattgtgat aaaattaatt tatagatatt aaaaaaagta taatatatgg tttttaagag
    16741 gtgctaagat gaaaaatttt agagatttag gtgggaacaa aactgaggat ggcagaactg
    16801 taaagaaggg attattttat aggtctgcaa aattatcaaa tctaagtgaa aatgatataa
    16861 agattttaaa agagttaaat attaaatata tatttgatta tagaagtgat gaagaagctc
    16921 gtaaacatcc atcaacaata atatcaaata taaaaaatat aagaatacca gcaatgagag
    16981 agctagaaga atcaggagga agttttggtt ctattgaaga tatgattgat ggattgtttg
    17041 aaaaagatgg tgcatttaat atgttaaata atagttacta taatttgcct ataaataatc
    17101 cgtcatataa aaaattagta gaacttataa gagactattc taatttacca atattaaatc
    17161 attgtacagc tggtaaggat agaactggtg tggggagtgc tataattctt atgattttag
    17221 gggtatcgag agagaatata atgaaagatt atctaaaaag taatgatttt gcagataaag
    17281 agatagaaag atttatagaa tataagccta aatttaagga cattccaaaa gaaaatttga
    17341 aatatatatt tggtgtaaat gaagagtata tgaaaactgc atttagaaga attgatgaag
    17401 aatacattag tgtagaagca tatttatatg gagaatttaa tttaaataaa gaagaaataa
    17461 gaaaattaag aaatcaatat ctagagtaaa aggtgagttt atgagtttaa atataacagc
    17521 aagacagata aatataataa aaatattgtt aaattcaaaa gatacaatta gtggtattgc
    17581 cttaagtcaa gagataggat gttcttcaaa aactatacaa aatgaaataa aggatattaa
    17641 taaacaactt agaaatggta aaattttgtc aataagagga gttggttata aactagaagg
    17701 aagttttgat gaggtaaatc taaatagcaa tctctatgat gatgtagata gagtagaata
    17761 tataatcaga aaaatattaa ccttaagtaa tgaagaaaaa aatactataa aacttgaaga
    17821 tttagccgat tctatgtatg taagtgtttc aacagtaaaa aatgatttaa aagaagtaaa
    17881 aagaatgttg gaaaagtatt caatatcagt taaatctaag cataaacaag gtatatgtgt
    17941 tttggaaagt gaagataaga tattaaattg tatagttgac ttaagcaata agagagataa
    18001 tcaattgagt ttaaatgatt ttttaaatga tgatataaag gaaagaaaac tattaataaa
    18061 aaaattatta ttagacatat taaatgaaaa tggattagta ttaactgata tagaatttaa
    18121 gagtgtgtta aataatatat tagttatgtt aagcagacat aagtataatg aaaaagaatt
    18181 tatagagaat tatatagaaa attataggag caaaagaagc tcaattttag aaaatgaaca
    18241 aaacaaagaa attataataa attcaataaa aacattt
//
LOCUS       DAELTA010000079        17944 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.079, whole genome shotgun sequence.
ACCESSION   DAELTA010000079 DAELTA010000000
VERSION     DAELTA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.079"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            34..1314
                     /locus_tag="KPG13_002193"
     CDS             34..1314
                     /locus_tag="KPG13_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG0985908.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1577..2968)
                     /locus_tag="KPG13_002194"
     CDS             complement(1577..2968)
                     /locus_tag="KPG13_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG0985909.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2979..4199)
                     /locus_tag="KPG13_002195"
     CDS             complement(2979..4199)
                     /locus_tag="KPG13_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG0985910.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4213..5001)
                     /locus_tag="KPG13_002196"
     CDS             complement(4213..5001)
                     /locus_tag="KPG13_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG0985911.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5018..6292)
                     /gene="larA"
                     /locus_tag="KPG13_002197"
     CDS             complement(5018..6292)
                     /gene="larA"
                     /locus_tag="KPG13_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG0985912.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6410..7123)
                     /locus_tag="KPG13_002198"
     CDS             complement(6410..7123)
                     /locus_tag="KPG13_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG0985913.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7248..8507
                     /locus_tag="KPG13_002199"
     CDS             7248..8507
                     /locus_tag="KPG13_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG0985914.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8787..9353
                     /locus_tag="KPG13_002200"
     CDS             8787..9353
                     /locus_tag="KPG13_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0985915.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9554..10759)
                     /locus_tag="KPG13_002201"
     CDS             complement(9554..10759)
                     /locus_tag="KPG13_002201"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG0985916.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10862..10968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11060..11428)
                     /locus_tag="KPG13_002202"
     CDS             complement(11060..11428)
                     /locus_tag="KPG13_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG0985917.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11480..12757)
                     /gene="obgE"
                     /locus_tag="KPG13_002203"
     CDS             complement(11480..12757)
                     /gene="obgE"
                     /locus_tag="KPG13_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG0985918.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12868..13158)
                     /gene="rpmA"
                     /locus_tag="KPG13_002204"
     CDS             complement(12868..13158)
                     /gene="rpmA"
                     /locus_tag="KPG13_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG0985919.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13172..13489)
                     /locus_tag="KPG13_002205"
     CDS             complement(13172..13489)
                     /locus_tag="KPG13_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG0985920.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13504..13815)
                     /gene="rplU"
                     /locus_tag="KPG13_002206"
     CDS             complement(13504..13815)
                     /gene="rplU"
                     /locus_tag="KPG13_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG0985921.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13916..15337)
                     /locus_tag="KPG13_002207"
     CDS             complement(13916..15337)
                     /locus_tag="KPG13_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG0985922.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15355..16056)
                     /locus_tag="KPG13_002208"
     CDS             complement(15355..16056)
                     /locus_tag="KPG13_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG0985923.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16058..17902)
                     /locus_tag="KPG13_002209"
     CDS             complement(16058..17902)
                     /locus_tag="KPG13_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG0985924.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5700 a         3287 c         1920 g         7037 t
ORIGIN      
        1 atataaaata attaaagata aaaggagtat tttatgaaca tacttggatt aatagttgaa
       61 tataacccat ttcataatgg acacatatat catttatcta aatctataga aaaaacaaat
      121 gcaacacata ctgtagcaat aatgagtgga aattttctac aaagaggcga accagcttta
      181 tttgataaat acactagagc aaaaattgct gtcgaaaatg gtgtagactt agttatagaa
      241 ttaccaactt tatttgcttg tcaaagtgct gaaatttttt ctcatggtgc tataacaatt
      301 ttaaattcac taaactgcat aaattctata tgttttggta gtgaagaagg aaatacagaa
      361 attctttata atctagctga aattttagca aatgaaccaa atgaatttaa gttactttta
      421 aaaaaatatt tagatgatgg tctgttattt ccaacagcta gagctaatgc actttttgat
      481 tatatacatc aagacaaaaa aaataatttt atacaaaatt attcaaagga acaattatca
      541 aaaacattaa attcatcaaa caacatactg ggtatagaat acataaaaaa cctcatccat
      601 ttaaacagta gtataaaacc atttactatc tccagggtcc aatcagaata taattccgaa
      661 gaaattgaga gtgatatttg ttctgctact gctataagaa cttcacttaa agaaaataca
      721 gacctttttc atttaaaaaa tgttattcca gaaaaaactt ataacatcct aaatgaaaaa
      781 atagataatg gttttttacc tatgtttgat gacttcttct ttgatatttt aaaaacaatt
      841 atacttagag atatggacac tttaaaagag tattttgaag taaatgaagg catagaaaac
      901 agaatctata aagatatctt tacatctgaa tttctacatg aactacaatt agcaattaaa
      961 tccaagcgat acactttaac caaaatcaaa cgaactctaa acaatatatt gttaggtatt
     1021 aaaaaaagcg acgtgcaact tataaaaaac atagatacta taccttatat aagaatactt
     1081 gcttttaatg ataaaggtag agaaattcta aaagagataa aaaataaatc tgaaataaaa
     1141 ataataaaca agttttcaaa tatatcattt tcattagatg atacgatttt taaaacactc
     1201 ataagttatg atataaaagc tacaaatata tataattcaa tatattataa aaacaataaa
     1261 gatttagtaa aaggccctat ggatttttat actcatccaa tatatgtaaa gtagtttttt
     1321 aaaataacta tatttataaa aatcatttta ttaactcttt attatataag atacaatagt
     1381 cttactttat ttatgtacac atgcttttag gatgacaaca tcaacttaaa tattatgtta
     1441 ctttctaaat ttaagtaaat attgccatcc taaaaatttt acaatttagc ttcagattta
     1501 aaagtaaagt ttaaaatatt atatttaaat ctaatttaat attttttgtt ttataaatat
     1561 tattaaatta atatttctaa tatattttcc ctggatttaa aatattttta gggtcaaaag
     1621 ctaactttat atttttaatt aacatcatat aagcatctga atttgattca tgtaagtact
     1681 cttttttagc atatcctatg ccatgttcac cagaaacttg accactaagt tctctagatt
     1741 ttttatacat atagtcaaaa gtttctttta atcttatttt ccatgtatta tcatccatac
     1801 catcttttaa aatatatatg tgtagatttc catctcctgc atgaccaaaa ctttttattc
     1861 ttatttttaa tttgtcttga agctcgtgag tgtatcttat aaactctgct atcttatctc
     1921 ttggaacaac gacatcacat tcatccattt gtgtcgtaga tgcctttata gcttctaaaa
     1981 atgcacctct ggctgaccat atagaatcgt ttctttcttg tgtatctgat ataaatacat
     2041 ctaatgcacc attttctaaa cataaattag ctactttttc atattctttt tctatatctt
     2101 cagtgctatt tccatcaaat gtaagcaaaa gataagcatc tgatgtatta tctggaaact
     2161 ttttacctaa aaattcttct gctgccaata tcacatctct ttccataaat tctatagccg
     2221 ttggtattga ttttgatttt attattttag gaacagtttc tattgccatt gataaatctg
     2281 gaaatggtat aagcaaactt atacttttct ttggtaaagg aagcaatttt aatatagctt
     2341 tagtaactat tcctaaagta ccttctgagc ccactaataa atcctttata ctatatccag
     2401 aactattttt aaccaccttt ccacctacat ttataatttc tccatttggt agtacaactt
     2461 caagtcctct tacataatcc cttgtaacgc catattttac tgctctcata ccacctgcat
     2521 tagtgtttat attacctgca attgtagcac ttttttcacc tgggtcaggt gggtaaaata
     2581 aatctctatc ttgaacatat tgacctattg tcatcaataa aacacctggt tctactgtca
     2641 atgttaaatt ttcataatca acttcaagta ttttattcat tttacataga ttaatcatta
     2701 ttccaccatt tatggcaaca gcagcaccta caagaccagt accttgacct cttggtgtga
     2761 ctggtatatt atttttatat gcgtatttca ttattttaga tacttgttca gtttcaagta
     2821 catttactaa aacttcaggg tacttctcaa tccctccaag ttcatcatgt gaaaaatctt
     2881 catttatatc acttccaact aatacatttt cttctccaca agtatctatt aaaaaatcta
     2941 tatctttttt atcgattaat ttatacattt ctatccccct atttagctgc tgatgccata
     3001 tatttttgat tattatcttt gttattttct attttttcaa tcaatttagg aataatatca
     3061 taaatatcac ctattaaagc taaatgtgct acatcaagta taggcgcttt atcatcttta
     3121 tttattgcta ctatatagtc agcaccctgc atccctgcaa caaattgaac tgctccagat
     3181 acaccacaag ttattatcaa tttaggtttt acagttctac cactaagacc tatttgtttt
     3241 ttagcatcta tccaacctgc ttctattaca ggtcttgttc ctgccacttg agcgtttaac
     3301 ttatcagcta atttatatat catctccata tcttcttgtt tttttattgc tctacttgcc
     3361 acaactatca cttcagcttc ttccagacca acttctttat ttttttcttt tacactcagt
     3421 accttaattt tagattttag ttcttttttt tctactttac ataaagttat ctttcctgta
     3481 gtattctcta ttttttctgg tgctgaaaat attttatatc ttacagtggc aaattgtggt
     3541 ctattattag gtgtgtttat atgagccatt atatttccac caaatgctgg tcttatttgg
     3601 tctaaatcag tatttgattg aatatcaagt attgtacaat ctgctgtaag acctgttcta
     3661 aatctagcgg caagtcttgg agctaatgac ctacctaatg tagttccacc aactaatact
     3721 atcgttggtt ttattttatc tataaagttt tctattgctt ttgaatatgg ttctatccta
     3781 aaatctttaa attcatcttc atcatataca aatacttcat ccaccccata agaaatcaaa
     3841 ttactgcact tatcttttat gtcttttcct acaaatacac aatacacagg ttgttttatt
     3901 tttgatgcca tctcacgagc tttacctata agctcgtacg ttacaggatg tatatttcca
     3961 ttatgatgtt caacatatac tgcaattccc ctccattcat ctttattaat ttgtacttta
     4021 ctactctcta taaattcaaa tgcgcctttt ggtccacttt tgacacacaa cttgcacatc
     4081 ttacagccag cacttatttc tactatacct gacttttctt ctatagcccc aaaaggacat
     4141 atttctaaaa ttctctttaa gtcagttatt ttatcttgat ttacaactat ctttgacatt
     4201 tattactccc ccttacacca actttaattc ctctaattta tttacaagtg caaaacttag
     4261 ttcatctgca tttcctctta caatattttt atctgtattt ggctttggag gaaatattct
     4321 tacaacttga gtaggagaac cattaagtcc atagttattt tcatctttat cttcaaagtc
     4381 atctaagcta tatacaggta tttctctatc tttagtatta agttttaatt taaatgaagg
     4441 tagtcttggt tgaaaaatgt ctttttctac agttattaaa catggatatt caatctcaca
     4501 tacttgtaaa tcatttggca tatctatttc aacaactata ctttcatctt taacttcaat
     4561 taattttgta acgttagtaa catgaggtat atctaaaaat tcagctactt caggtcccac
     4621 ctgtgctgta tcaccatcag tagtttgttt tccacaaatt attaggtcta cctttcccat
     4681 cttcttaata ccttgtgata tggtatagga agtagccaat acatctgctc ctccaaatct
     4741 cctatcactt attaatgctc cttcatcagc tcccatcata aaactttctt caattacctt
     4801 ctttgcctga ggaggaccca tgctaagtac ttttagattc gctttcttat cttcctttat
     4861 tctaagagct gtttctaaag catataggtc atagggattc atttttgaat ctactccatc
     4921 tctttttagt actcctgtag tttcatcaac ttctactgaa gtcgttccag gaacttgttt
     4981 aatacatgtc aaaatttcca taaagtacac tcctttttta tttaactata actgatactc
     5041 catcaggtat aactactact ttgctctcct taccaactat actattcgcc atttctaaag
     5101 cttctttaaa gttaaatgca tgtttcatat gcattttttt tatcatttca ggattacact
     5161 tatctgttac cattatcaca gtatatttag ataatattct cgctagtatc tgaaattccc
     5221 attggtctgg aatcgtatct actctatcga cacttctaat tttatctaaa agttgttttg
     5281 gagaatctgc atttgctaca ttttcataaa aagattgacc tccatgacca tcattacatg
     5341 ctgaaatcat tattattact ccaccgtctt tacaagtagc ttcagctgct gtcattcctt
     5401 taacagattg ataaatattt tggtctagtg gatatccacc atttgtagtt acaacaatat
     5461 cagattttat agaatttact tttgataact ctgttacaaa tctacatcct ttagtatgtg
     5521 ctttttcgac atcaccagca aaggaagcaa ttattttttt atctttatct attacaacat
     5581 tcaatatgaa tgctaatttt gcaactttag ctgcatatac catatcctca tgaataggat
     5641 tattattcaa ttttccagtt cttgaattat cactatctat aaagtcacta caatgattgt
     5701 acattatagt ctttgcagat gcgattcctg gaagtacact ttttctgcca cctgaaaaac
     5761 ctgcaaagaa atgaggttct ataaaacctt ctgctatcaa aagttctgcc tcatatgcta
     5821 gtttgtttat atataaatct ccacctgaag gcaatatccc tactttacac atagatttat
     5881 catcagtaga tatatgcata actatatctt cattatctac tatttcttct cccattttat
     5941 aaattagctc ttcccttgta cttggcctat gaaatccagt tgccactata atcttcacat
     6001 caatttctgg attaacattc ctaatccttc ttaatataat tggcatagtt attctacttg
     6061 gtacaggtct tgtatggtca cttgtaatta tgactacatt ctttttcccc ttaaccagtt
     6121 cttccagaga attagagcct ataggattat tcatagattc ttcaactatc tcttcttgag
     6181 tcttatgttc tatataatta tccgattttg actccaaaac accgagaaaa cgttcatcag
     6241 gtatttctaa aatcattcct tgttttgaat acggtacttt taactttacc atttttaatc
     6301 tcccattata ataaactata aaattggtat gtggtcatac cagttttaca tttatatgat
     6361 agtaccatta tctaccattt gcaagaataa tttattttat aacaaacttt taattttcat
     6421 tttttctata atttttactt ataaaatcca tatgttcctt tgctttctca catgcaagtt
     6481 ctacatcatt ttctacaact gcattgtata tagcttcatg ctgtttaagc aatatatttt
     6541 cattaaaagg ttctgcgatt attttttctc ttgcatcttc tataaatcca tcaaataatt
     6601 ttgatgctgt cataaaaaga ctttgaatta aataattttt acttaatgtt gctagtttac
     6661 tgtgaaattt ttggtctaat aacaccagta ctcgattatt atctttttta ttagtttcta
     6721 ccctcatttc atctattata tgtttcaatt ctataacttc agaagaagtt attctctttg
     6781 aagcaagttt tgcaatctca atttctagga ttattctaag ctctaatata tcttcaagtg
     6841 taccattatt aagtttaaaa atcatagata aaggttctat taaagttttt tctatatttg
     6901 tacatataaa atttccttca ccctgtctgc tttctatcac tcccatagtc tctaataccc
     6961 taatagcttc ccttatggaa gttctactca cattcatttg ttcagacagt tctctttctg
     7021 atagtaattt atcccccttt tttaactctc catttagtat tttatattgt atttgctcaa
     7081 ttacttgttc ataaactttt ttactagcta tactattaaa cattttttcc tccaatatga
     7141 taatcgttgt tgtaatatat atctattata tatcttaagc cattacattc atatattttt
     7201 attactttta atataatatc ttataactca aatttacagg tgatattatg aaaaaaaata
     7261 aaattattgc tttttttgct ccatctatca tagttggagt tctagtttta gggattatcc
     7321 tatacccaaa tgaagctata aaatctgcaa gagaaggctt ttctatatgg tctaatgttt
     7381 tagttccatc actactacca tttataattg gagcaaactt aattgttgat ttaaaaatag
     7441 tggatattat agggtttatc atcaatccca taactagatt tgtatttaat gtttcaggaa
     7501 agagcgcttt agcttttgct atatctacag tatctggtta tccagtagga gtaagtttag
     7561 cttctgaatt taggagtaat ggacaaattt ctaagtatga agcacaaaga cttgtgtctt
     7621 tttgttctac atcaggacct ttattcataa taggttcggt tggtacaggt atgttccaaa
     7681 attcaaactt aggttattta atgattctct gccattatct aggaactata ttggttggtc
     7741 ttttctttag aaattatggt aatgaaaatc ttccaaagac aaaattggat ttaaaaacaa
     7801 atataaaaaa tgtaatcaac attagaaatt caagtgggaa tggttttttt gttttatttg
     7861 gaaatgctgt atttaatgga gtaaatacat tacttacagt tggtggattt gtaattgttt
     7921 tttcagtagt atttaaaata ctatctcttt ttaatgtaat ctcattaata gcatcagtta
     7981 tatatttgcc tctttcattt ttaggtgtta gtaaagaact ctgtcaagca tttgtaagtg
     8041 gtctatttga aattactata ggatgcaata aagtatctag tataacaagt tctccagaaa
     8101 ttttaagagc atctttagct agttttttga taggatttag tggattatcc atcctagctc
     8161 aatgttgtac atttctatca aaaacggaca tagatttaaa attatacata ctaagtaaat
     8221 ttgcgcatgg agttttagct gctatcttta tatttatact ttatccaatt gcaaactctg
     8281 ctgtgttggt ttctagcttt gctgatacat acaacgttat atataataat ttaatctggt
     8341 tttattatct tagctattat gatacaattc ttcaaattgt agtaataatt tatctaatct
     8401 cagccgtatt tattgcaaaa aaacatatga aaactttatc tacaaatggg aaactaatgc
     8461 gatataaaaa tacttttttc actaagaaat tttcacgaaa aaaataaaac taaaaataaa
     8521 actaataaaa cttaactaaa tacttccatt tttttattat atttgtcata attttaataa
     8581 cctttttaat tagtttaaca gcatagttat ttcatcacaa tttaataatt ttattagaaa
     8641 tatttttcaa aaaattctcc ttaatcacat atatcacact tgtataagtt tactgggaaa
     8701 tttttccatt atataatatt tcatttatat tatataataa ataccgacct aaacttacca
     8761 aatcaaacta agaggagtgg ttgaaaatgg caacaagacc tatagaaata gacgaatata
     8821 aaaagataat ggaactatta catactggct ttacttatag tgaaaatggc gttgaaaaaa
     8881 gatttagaaa aaatcctaaa gttgcattgg cactaatgtt agaagctaat ttaggtctaa
     8941 gaatatcaga tattttaaga ttaaagatag gtaattttaa aggtaatatg ttggaaataa
     9001 atgaaaaaaa gacgggcaaa ttacagtata gaccagttaa taaaaatata attgagtcca
     9061 taaatgaaca tgcaagaaag tataatctta aatcaaatga ctacttagta aatataaaga
     9121 caaaggctat tcaaaaacag ttgaggataa tttgtaaata cttaaatctg tataatataa
     9181 gtacacatag ctttagaaag ctctatgcta ctactcaatt tgaaaagagt aacaataatt
     9241 tagagttggt taaagaactt ttaaatcaca gctctgtagc cactacacag cgatatataa
     9301 gggtaactca gcaagctatt aatgaagcta gtgaaaattt ctttattggt taattataac
     9361 gtatacattt ttctattatc ttattaattc accagatatt aaaaaagagt gtctcaaaat
     9421 gaacttttta gttcatgaga cactcttttt ttataaaatc aaatatattt ttattatttc
     9481 aacactggaa aaaaggctta tctctctatt ttgagacacc ctattaaatt ttcataattc
     9541 ttgcataaac atattaatct atcttcaata attttttagc tgattcactg taattatttc
     9601 tttttacaat ttccaaaact ctatcaatct catgttctga ctttgcactt acaaaaatcg
     9661 ccaattcttc atcatctata atatcataac tatctacata tttatctaaa cattctaaaa
     9721 ttccatcttt attattatac ccaccaaatc taactactgc actacactca tcttctataa
     9781 gtccacttaa atccaatttc ccttttgaaa aagactttat atttgaactt ctattatcta
     9841 ttatatctat aatatctgca taaacagcac ttcctgttgc acctcttcca gctcccttac
     9901 ctacaaaaga aagttcacct actgaatcac ctactaaaat aactacgttg aactcattat
     9961 ctatctttga aagcatatta ttattgtcca ctaatgctgg tctaacaaat ccacttactt
    10021 ctcctgattc atacttactt ctagcaacca attttatctt acaatttaat ttttttgcat
    10081 attctatatc tttttcatct attgaagtta ttccttctaa taataaatct ttccaatata
    10141 ctatttttcc ataagataaa gtagacaata tagataactt tctagcacta tcatatccta
    10201 aaacatctgc atctggatta acttctgcaa atcccaactc ttgtgcctgt tttaaagcga
    10261 catcatacgg taaattttca tcatacatct ttgataatat aaaattacaa gtcccattta
    10321 aaatagcgtt tatgctcttt atattgttgc cttccagtga ttctatcaat ggttttaaaa
    10381 ctggaatacc acctgccaca gatgcttcaa acttgatgct aacattattt aagttagcta
    10441 attctataag gtcactacct ttttcagcca gtaaatcttt attagctgtc actacatgta
    10501 tttttttatt taacgctctt gttacataat caaaagctgg atttaatcct ccaattactt
    10561 caactagtat atcaatatcc ttgtcaaata tatcttctat atcagtagtt atcttatcaa
    10621 aatacttctt atacttgtgt ttttctttat ttttaactaa aattccaaca acttctatat
    10681 ctctttttga agtattattt tctattaaat caattaatcc acttcctact gttccatacc
    10741 ctaatattgc tatcttcact gtaacatacc cccatcattt tgattatatt aaattattta
    10801 tatatataaa tataccaata aaaaaagctt gagtataaac tcaagccata tgacgggttt
    10861 atactcatct ttcagcataa atgctgcagg atttagcacc aatgtataat taaatatact
    10921 ggttgccggg cttcacaggg ccagtccctc tgccactctt gataagattg ctaatttaaa
    10981 tttatgttta atagtataat acaatttctt ccaattgtca ctattttttt aacttatttc
    11041 cctaaaagga tataaactat tatctaacag ctcttttagt tggtttaccc ttttttgtaa
    11101 cattattatt tttagtcttt gctttcgcct gttcatgtcc tggaaaaagt attttaggct
    11161 cttcaatatt cctcttgtaa atagtaaatt taaaaccaat agcctgcaca aattcagctc
    11221 ttaaagcttc acatactgca ttagcagttt ctttagtatc aagacctgca ttttctaata
    11281 tagtaacttt tactaattct ctagttctta acataccatc taattgctct aagaaactct
    11341 ctgtaacacc ttctttacca atttgtgtag taggctttaa agtatttgcc aaagatctta
    11401 aatacgccct ttgttttcct tttaacatat tctcacctct ataattctat acttaaatta
    11461 tctataatct atatttaaat taattgtaaa actcaaattc taattcataa attttaacca
    11521 catctccatc ttctattccc atttctctaa gcctatcaaa aacaccttga ctctccatag
    11581 ctttttggaa atattgaagg gattccatat catcaaaatt tacagaatac attattcttc
    11641 taagtgcttt acctgtaact acatacacac catcttcaat atctatactt aactcttctt
    11701 cagtaccaat atcaagttct ggtctataca tttcttcttc tgaaactagc tccacatctt
    11761 ctacttcttt tagcatttta gaaacatagg ctattacttc atctatgcct tgacgagttg
    11821 ctgctgacat tttaaatact gtatatcctc tgccttctaa ttcactctta aacttctcaa
    11881 acttactctc atcttctaat atatcaaatt tatttgcaac tacaacttgt ggtctctttg
    11941 aaagcttttc attgtacaat ttaagctcat catttatctt gtcaaaatct tcaatagggt
    12001 ctcttccctc taatccagat atatctacaa tatgtatcaa tacctttgtt ctctcaacat
    12061 gtcttaagaa ctcatgacct aatcctatgc cttcagaagc accctctata attccaggta
    12121 tatctgcaag tacaaaacta tctccaaact tagtttgaac aacacctaga tttggtgtaa
    12181 gtgttgtaaa atgatagttt gcaattttgg gtttagcttt agttacaact gatagaaatg
    12241 ttgatttacc tacatttgga aatcctaaaa gaccaacatc tgctatcatt ttaagttcaa
    12301 gagttatcca tctttcttct ccatctgtac cagattttgc aaaagctgga gcttgtctta
    12361 ctgcatttgc aaagtgttgg ttacctttac cacctatacc accttttgca actatagctg
    12421 tatctccttc tttttttaaa tctgctaata caagacctgt agcctcatct cttattactg
    12481 tacctgctgg tacttttaat actaaatttt ctccattttt accagctctt tttcctttgc
    12541 ttccgtcgcc accattttga gcttgatatt ttttctggta cttaaagtcc attagagttc
    12601 taagccccaa atcaacttca aatatgatgc ttgctccacg accaccatct ccaccgtctg
    12661 gaccaccagc tggaacgtat ttttctcttc taaatgctac cgaaccattt ccaccatttc
    12721 cagccttaac aaatattctt gccttatcta taaacaaatt cttcacctac ttaattaaaa
    12781 acttttaatt agcattacca aaaataccat tataaattca tcagtattat aagaaaaagg
    12841 agtgcctcaa cactcctttt atgtatatta ctcagctatt gctactggat atatactaac
    12901 tttttttctc ttcttgtctt ttctttcgaa tttaacagta ccatcaacta atgcaaataa
    12961 agtatcatca ctaccttttc caacattagt tcctggatgt attttagttc ctctttgtct
    13021 aactattata cttccagcag ttactaattg accatcgaat ctcttaacac ctaatctttt
    13081 agatatagaa tctctaccat ttttagaaga acctacccct tttttagatg ctaataattg
    13141 taaattcatg tttaacatat ggagtcaccc cctacttttt taaaattttt atattttttg
    13201 ggtattcccg tttaatttct ttaaccgcta attgaaagtg atttagtaat aattgtgcgt
    13261 cttttacatg tgtatctttg actatacact ctatatgacc ttcttctcct actacttttt
    13321 caaattcaat cttctgtaat ttgtctaaag aatttataac tgcaatagca ttagatgtaa
    13381 ctgcagcaca tactatatca tagcctattt cagcaaaatc agcatgtccc tttaaacaaa
    13441 aaccttgcaa caagaaatca tcattataat aatatttaac ttttatcata acttaaccaa
    13501 taactaagcg tttatttttt ctacaactat cttagtgtat gattgacgat gtccttgttt
    13561 tcttctgtaa tctttttttg ctttatactt gaaaactata actttcttag ctttaccttg
    13621 ttcaacaact ttagcttgaa cagatgctcc ttctactact ggagaaccta attttaattc
    13681 tccatcttta gagcaagcta atacttcgtt taaagtaaca acatcacctg cgttagcttc
    13741 taatttttca acgaataata catcaccttc agaaacttta tattgctttc caccagtctt
    13801 tactatagcg tacattaaat acacctcctc ggcctcaaac tcgccagtac aaggtacttc
    13861 atttgaagat ttaaaccttt actgtgcggc attacaatta ttaattataa aatatttaca
    13921 ttttattgtc aactatttta tcgaaaataa cattggtttt attgtgcttt aatgtactat
    13981 ttgcgtttaa agaaatcttt atattatatt tttcaccaat tttatcaatt atatccatat
    14041 aatattcttt tatttgttca aaaataaaat cattaaattc taccattatg tttttgtaaa
    14101 cagtatgttc acttattctc ataatttctt tttcaatact atctagtata taatgtacag
    14161 attttattgt ttgttcacca tcacaacatg gacaagacat taaataatac ttatcaatgg
    14221 attctttttc tctacgccta gcaacttcta ctaaaccaag ttttgtcata cctaatactt
    14281 cagcttttct tttatcttta ttaaattctt cttctaggat gtgtaaaatc tccttttgat
    14341 gcttcttttt atgcatatca ataaaatcga ttattattat cccagctatg tctctaattc
    14401 taagctgtct tacaatttct tttgcagctt caagattagt tttataaacg gtttcatcaa
    14461 gtttaccagt tccaataaac ttacccgtat ttacatctat aaccgtaaga gcttctgttt
    14521 tttcaattat taaataacct ccactcttta accatacttt tttatttaat aatttttcta
    14581 ttttagattc tattctgtat aaatcaaata catctctatt ttcctcaaga cacagtttat
    14641 caacataagt cttatctata cctcttaaaa tagacttaag ttcagagtac ttatcataac
    14701 tattggtaat tatagattca atatcatcat tcacattatc ttttacatat ttagttgcaa
    14761 aatctaaaga cttatataat aatttcggac ctataccaag tttatattct tttaaaatgt
    14821 tttcatactt taatttcaac tcttctatat ctttctttat ttcatcatga gtgcaacctt
    14881 gagcctcagt ccttattatt aatcctagat tttccttatt tactgcctta gtaatttttt
    14941 ttaatctaaa tcgttctttt tcatcagtaa ttttatttga tattgtagtt ctatcattgg
    15001 aaggtatata gacaatatac cttccagaaa tactaatctc agtgtttaat tttgcgcctt
    15061 tagttcctat ttcttcttta tttatctgaa ccaaaatatc ctgaccagat tttatagtac
    15121 ttcccttttt taattgtaaa taagcaagtt tcttaaatcc aatatctaca aaacaagctt
    15181 ctattcctgg tataactttt tttacgattc cacgatatat attggataca gtcttcttat
    15241 ttaaattgtc ttcaacaaaa agttctgtta atctttcatc ttcaagtact gctgttttct
    15301 gggaccctat tagggactca ataactatct ttttcacaac ttcacctctc gagcttacat
    15361 aggagtcact aattccccat cttctaatac atataaatct cttctcaata tatcaacatc
    15421 taatgggtca atatcaagtt caaacatctc taatatcttt ggtataagta tattagtatt
    15481 taaattagtt tttgagcctg tagctatagt agcagtaaga gtaatatgtt catcttctaa
    15541 atttaaaaca tcaaaggttc ttatcatagg tcttatatct acttcaacca tcttaccttt
    15601 tttattcttt tttgttatga ttatttcttt attattcata aagtcaatta cttttctttt
    15661 tacaaactct ttcgtcatag gtcttttcaa atctatatta aacaaatatt ctccatagtc
    15721 tatcacagaa gaaagagaag gtgtttgtct atcaatttct tttgctttga taaaatctat
    15781 cccatctgga agttgaattg atactttatt taaaaattcc tctactgata aatcatcttc
    15841 ttctatttca atatctacat actcaccttg actttctgcc cctaaagcca aagcattgcc
    15901 ataactcatt ttagggtgag gattaaatcc ttgagaatgt gataagttta tttcagctct
    15961 tctaaacgct ctttgtaaca atctctgaag gtcaagatgt gatatatata tcatgtcgcc
    16021 ttccttttta aattttactc ttattatctt actcatatta gcaaagcccc cttcctatat
    16081 catgtatatt tattccacaa ccattgcagt tatgtctaca atcagaagtg attttttctt
    16141 ttttagcatt ttcgtcttct ctaattaaga attttttact aatacctaca tctatatggt
    16201 cccaaggaaa tacctcttca tagtttctat ttctatgtgc ataaaaatct atagaaagat
    16261 tattcttttc catagcttct ttccatatat ctaaattaaa atgttctgcc catccatcaa
    16321 atttagcacc tagtctaaat gcatcatata taacttttcc tattcttcta tcgcctctag
    16381 ctactactgc ttccatcaaa ctagtctttg aatcatgata attgtatttt atattgttat
    16441 ttcttaattt atttactaaa tgtctctgct tattaataac ttcttctgta gtatcttgac
    16501 catgccattg gaaaggtgta aatggctttg gtacaaatgt agaagtactc actgtaacac
    16561 taaaacttct ctttagcttt cctccattta cttttctata aatatcaata actttataag
    16621 ccaatttagc tataccatct aaatcttcat aagtctcagt tggtaaacca atcataaaat
    16681 acaacttaac actattccac cccatctcaa aagctctttc tgttgcattt tccaaatctt
    16741 cttctgtaac acctttattt ataacatctc taagtctttg agttcctgct tctggagcaa
    16801 aagtaagtcc agattttctt acttgttgta ttttatcagc aatttccatt gaaaaattat
    16861 ctaatctaag ggatggaagc gaaatcccaa tattattaga tgcatattca tctaccaaat
    16921 aatcagtcaa ctctgataac ttactataat cactcgtact aagtgatgat aatgatattt
    16981 catcatatcc agtattctta actaatttat ctaatatttc tttaagtctt ttaacactct
    17041 tttctcttat tggcctgtat atcatacctg cttgacagaa tctacatcct ctagtacatc
    17101 ctctaaataa ttctagtact attctatcat gaacagtatc tatataaggt actattagtt
    17161 tttctggata atctacagtc tcaacatctt ttattatcct tttagtagga tttttagtaa
    17221 tcccttctct attaggagtt acactttgaa ctgtaccatc ttcattgtac ttgacatcat
    17281 aaaaacttgg tatgtatact ccttctatgc tagatatttt atataaaaaa tcttctctag
    17341 tcgttttatt tttcttccat tctttatact catttactac ttctaaatta acctcttcac
    17401 cttcacctaa tattactaca tctacaaaat ctgctaatgg ttcggaatta taagcacaag
    17461 gaccaccaac cattataaat gggtcttcca aagttctttc ttcttttaat attggtatat
    17521 tggctaaatc taatatattt aagatattag tgtatgaaag ttcatattga agtgtaaaag
    17581 tgacaaaatc aaagtttgtt ataggctctc ttgactctaa tgcaaataat ggtatacttt
    17641 tttctctcat tatattttcc atatcaactg caggagaata cactctctca caaaatacat
    17701 cttcatcctt atttattaca tcatataata tatgactccc taagtgactc ataccaactt
    17761 catataaatc tggaaagcaa tgtgcatatc ttatcaattc actattacta gtatctttat
    17821 gaattgaatt tatttcattt ccaagatatc tagcaggctt ttctactttt tttaagattt
    17881 ttttcaagtc tactttattc atttattaaa attcctccaa acaagctctt tatttttatt
    17941 ctta
//
LOCUS       DAELTA010000080        17637 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.080, whole genome shotgun sequence.
ACCESSION   DAELTA010000080 DAELTA010000000
VERSION     DAELTA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.080"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            72..1676
                     /gene="rho"
                     /locus_tag="KPG13_002210"
     CDS             72..1676
                     /gene="rho"
                     /locus_tag="KPG13_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG0985925.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            1829..2029
                     /gene="rpmE"
                     /locus_tag="KPG13_002211"
     CDS             1829..2029
                     /gene="rpmE"
                     /locus_tag="KPG13_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG0985926.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            2174..3067
                     /locus_tag="KPG13_002212"
     CDS             2174..3067
                     /locus_tag="KPG13_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG0985927.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            3085..3933
                     /gene="prmC"
                     /locus_tag="KPG13_002213"
     CDS             3085..3933
                     /gene="prmC"
                     /locus_tag="KPG13_002213"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG0985928.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            3977..5041
                     /gene="prfA"
                     /locus_tag="KPG13_002214"
     CDS             3977..5041
                     /gene="prfA"
                     /locus_tag="KPG13_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG0985929.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            5170..5850
                     /locus_tag="KPG13_002215"
     CDS             5170..5850
                     /locus_tag="KPG13_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG0985930.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            5879..6919
                     /locus_tag="KPG13_002216"
     CDS             5879..6919
                     /locus_tag="KPG13_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG0985931.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            6936..7385
                     /locus_tag="KPG13_002217"
     CDS             6936..7385
                     /locus_tag="KPG13_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG0985932.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            7402..7854
                     /gene="rpiB"
                     /locus_tag="KPG13_002218"
     CDS             7402..7854
                     /gene="rpiB"
                     /locus_tag="KPG13_002218"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0985933.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            7890..8519
                     /gene="upp"
                     /locus_tag="KPG13_002219"
     CDS             7890..8519
                     /gene="upp"
                     /locus_tag="KPG13_002219"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG0985934.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            8814..9629
                     /locus_tag="KPG13_002220"
     CDS             8814..9629
                     /locus_tag="KPG13_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG0985935.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            9855..10292
                     /locus_tag="KPG13_002221"
     CDS             9855..10292
                     /locus_tag="KPG13_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG0985936.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            10477..10704
                     /locus_tag="KPG13_002222"
     CDS             10477..10704
                     /locus_tag="KPG13_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG0985937.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            10709..11095
                     /locus_tag="KPG13_002223"
     CDS             10709..11095
                     /locus_tag="KPG13_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG0985938.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            11098..11802
                     /gene="atpB"
                     /locus_tag="KPG13_002224"
     CDS             11098..11802
                     /gene="atpB"
                     /locus_tag="KPG13_002224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG0985939.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            11871..12131
                     /gene="atpE"
                     /locus_tag="KPG13_002225"
     CDS             11871..12131
                     /gene="atpE"
                     /locus_tag="KPG13_002225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG0985940.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            12254..12751
                     /gene="atpF"
                     /locus_tag="KPG13_002226"
     CDS             12254..12751
                     /gene="atpF"
                     /locus_tag="KPG13_002226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG0985941.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            12748..13293
                     /locus_tag="KPG13_002227"
     CDS             12748..13293
                     /locus_tag="KPG13_002227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG0985942.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            13310..14802
                     /locus_tag="KPG13_002228"
                     /pseudo
     CDS             13310..14802
                     /locus_tag="KPG13_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            14830..15708
                     /gene="atpG"
                     /locus_tag="KPG13_002229"
     CDS             14830..15708
                     /gene="atpG"
                     /locus_tag="KPG13_002229"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG0985943.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            15721..17115
                     /gene="atpD"
                     /locus_tag="KPG13_002230"
     CDS             15721..17115
                     /gene="atpD"
                     /locus_tag="KPG13_002230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG0985944.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            17118..17378
                     /gene="atpC"
                     /locus_tag="KPG13_002231"
     CDS             17118..17378
                     /gene="atpC"
                     /locus_tag="KPG13_002231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG0985945.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT         7007 a         1977 c         3448 g         5205 t
ORIGIN      
        1 tgtttaaata aaacttttct ttttcatgcc ttcaatttcc aaaataagta tgtatataaa
       61 tgaatatata gatgaggagg atttttttga ttttagatga taaagtatta gaaggattag
      121 gtagtaagac cttagttgag ctacgagaga tagctaaaga attaaaaata aaatcaatta
      181 ctacatataa gaaaaatgag ttaattgaga ttataaattc caaaagtaaa aatgaggaaa
      241 aagtaaacga agagactaaa gaaaaaaatg aagatattaa agaagacata aagaatagaa
      301 aagaaaatat agagcgtgaa agtatagaaa aagaagttga atataagagc cctaccaaat
      361 catacaacaa gaaagaaata gatacccaaa atatttccca aaaccaagaa catagaaatc
      421 aaagaaatga ctataataag acaacgagtg taaatgatag taataagtca tcaaatatat
      481 ctaatagaat ggtcagaaat aataataaaa actattatat gcctaaacaa gttgatgaat
      541 ctaagatagt agatgaattt aacacatcaa aagaggatga agtagtaggt gtacttgaaa
      601 ttctgccaga tggatttggt tttttaagag gttcaaatta tttgtctact gaaggagatg
      661 tttacgtttc accatctcaa ataagaagat ttaatatgaa aacaggagac aagattaagg
      721 gaataactag acatccaaaa agtggagaga aatttagagc acttttatat gttcaaaaaa
      781 taaatgatga aaaccctgac acagctatac aaagaaatgc gtttgagaca ttaacaccaa
      841 tatttccaga agaacgactt acactggaaa ctaatagaaa tgaaatagct acaagaatta
      901 tagatttaat ttcacctata ggaaaaggac aaagaggatt gatagtagca cctccaaaag
      961 ctgggaaaac agttctttta aaaagcgttg caaatagcat agctaaaaat catccaaatg
     1021 ttgaacttat agtactcctt atagatgaaa gaccagagga agttactgat atgaaagaat
     1081 ctattgaagg agacgttata tattccactt ttgaccaagt atcaagtcat catgtcaagg
     1141 ttgcagagat ggtgttaaat agagctcaaa gattagttga acatggtaaa gatgtagtaa
     1201 ttttactaga tagtataacc agattggcaa gggcatataa ccttacaata tctcctacag
     1261 gtagaacttt atctggaggt attgaccctg gtgcacttca tggacctaaa aagttctttg
     1321 gtgcagcaag gaatattaga cagggtggtt ctttgacaat attaggaaca gcattggttg
     1381 aaactggttc taggatggat gatgtaatat ttgaggaatt taaaggtaca ggaaatatgg
     1441 agctacattt agatagaaaa ttagcagaaa aaagaatatt ccctgcaata gatatttaca
     1501 agtctggtac aagaagagat gatttattgc ttgatgatga agaaaagact gctctttgga
     1561 gattaagaag agaaatgagt aataattcag taatggaaat tacagataaa gtcattgaac
     1621 ttataaagag aactaaggac aataaagagt ttgtaaaatc aataaagaat ttgtaaaatc
     1681 aataatacaa atgcaaatat ggattatata aactaataga aaaatcactt gaatggcata
     1741 ataagttgtg ctataataat gtagttgata atgtaaaaag aaagttaata taattaattt
     1801 ttttaaataa agagaagagg tgactataat gcaaaaggaa atacaaccaa aatacaatcc
     1861 agttgaagtg cgttgtgctt gtgggaacac atttgttgct ggttcaacta aggacgaaat
     1921 aaaagttgaa atatgttcag agtgccatcc attctataca ggaaaacaaa agaacataga
     1981 aaaaggtgga agaatcgaca aattcaagaa aagattcaaa atggactaat ttttataaaa
     2041 gtggagttgg aggaagccaa ctcctatttt attacaaaaa aacattcata ttctacttta
     2101 aattgtcttt ttgtattata ataataaata tgtaaaattg agtattctaa atacaataaa
     2161 atgaggtgag tggatggcaa aacaagctgt aggtgggcaa gctgttattg aaggtgtaat
     2221 gatgcagtca aaagacaaaa gagcggtagc tgttagaaaa agtgacggag aaatagcact
     2281 aaaagaagat agaataaaaa gttgggtaag agataagaat atagataaga taccttttgt
     2341 tagaggttct tttgttatga tagatacaat gattcaaggc ataaaaagtt taaacttttc
     2401 ttcagagttt tttatggaag aagctgaaga ggataaattt gatttattta taaagaaaat
     2461 ttttaaagat aaggcaaatg atataattat aatattttca ttggtcatag caatgttact
     2521 ttctgctgga ttattcattt ttatacctac attggtagga ggagcttttt ctaaagtaat
     2581 gcctaatgat tttatgctta atttaataga aggaatcata aggatagcta tattatttgc
     2641 atatatagta ttgatttcac gaagtaagga tatagaaaga gtctttcaat atcatggagc
     2701 agaacataaa tctatatatt gctatgaaaa tgatttagaa cttacagtag agaatgctag
     2761 aaaatttaaa agattacatc ctagatgtgg tacaaatttc ctatttatag ttatggctgt
     2821 atcaataata ttgtttgcat tttttggttg gccaaatcca attcttagaa tatttatgag
     2881 aattatatgt gtgcctatag tagcaggttt atcttatgag gtaattagag ttcttgggaa
     2941 atatgataat ggatttacta aaattattgc atatccagga atgatgttgc aatattttac
     3001 aacaaaagag cctgatgatg agcaattaga ggttgctttg gaagcattga aagcagtagt
     3061 agattagaga aaaggatatt tgttatgacg ataaaagata taattattaa atattcagat
     3121 aaactaaaag atataagtga tactccaaga ttagatacag agttattgtt acaaaaaaca
     3181 ttgggtgttg atagattata tattcactta aatctgaata aagagctaac tgaagaacaa
     3241 aaaacaaaat ttatgggttt tgcagaagaa agattaaatg gaagaccaat agcatatata
     3301 gttgaaaata gggagtttat gggattagat ttttttgtta aggaaggcgt attgattcca
     3361 agaccagata cagaaacttt agtagaagaa ataattgaaa tatgtagaga aaaaaaagat
     3421 gtaagtatat tagatatagg aactggttct ggagctatta caataagttt agcaaagtac
     3481 attgaaaatt ctaaaatcat gtcttttgat atatcagaga cagcattaga aatagctaaa
     3541 aaaaatgcta ttataaatga agttggtgaa aaaataaagt atattaattc tgatttattt
     3601 acagcaataa gtgatagtaa cataaaattc gatatcatag tttcaaatcc accatatatt
     3661 aaaaaacaag atatagaaac tctgcacaaa caagttaaag attatgaacc atataatgct
     3721 cttgaaggtg gagaagatgg attagacttt tatagaagaa taacagaaca aggtaagaaa
     3781 tatttaaata agtgtggaat attagcatat gaagttggtc ataatcaagc tgaagatgta
     3841 ataaatatta tgaaaagtaa tggatataaa aaaatataca caaagaagga tattcaaggc
     3901 attgatagag ttgttatagg ttacaatata tgatataatc aacaattgat atgttacatt
     3961 taatcgaggt gaaagtatgt taaaaaaatt agaggtattg gaagatacat ataaagactt
     4021 aagtgaaaaa ataggtgatc cggatgtaat aaatgatcaa aaagtttggc agaaatatat
     4081 aaaagaacat gctgacttgg aacctattgt tatgaaatac agagagtata aaagcgtttt
     4141 agatagtata aaagaatcaa aagaaatttt acaagaagag tcagatgaag aattaagaga
     4201 attagctaaa atggagttag ctgagatgga agaaaaagta gcacctctgg aggaagaaat
     4261 aaaaatacta ttattgccta aagaccctaa tgatgataag aacgttatag ttgaaattag
     4321 aggtggagct ggaggagatg aagcagctct atttgctggt gatttattta gaatgtactc
     4381 aaggtatgct gaaagaagaa gatggaaaat agaattattg agtgcaagtg atactggagt
     4441 tggtggatat aaagaagtat cttttatgat aaaaggtaaa ggtgcttatt caagattaaa
     4501 gtatgaatct ggagttcata gagttcaaag aataccatca actgaatctg gtggaagaat
     4561 acacacatca acttctactg ttgcagtatt accagaagtt gaagatgtag aggttgaaat
     4621 aaatccaaat gatttaagaa tagatgtttt ccgttcttca ggtaatggag gacaaagtgt
     4681 caatactact gactctgctg ttagggtaac acatatacct acaggtgaag tagtatcttg
     4741 tcaagatgga aaatcacaat taaaaaataa agagcaagct ttaaaaatat taaaagctag
     4801 actttacgat aaagcattgg cagaacaaca caaagatata gcagcagaaa gaaaaagcca
     4861 agtcggaaca ggagacaggt cagaaagaat aagaacatat aatttccctc aaggtagaat
     4921 aagtgaccat agaataaatt taactttata taagttagat gcattcttag atggagatat
     4981 agatgaaatg atagatgctt taattactgt tgaccaaaca gaaaaaatga ctgcaatata
     5041 agtaatgttt taaagaagct gtttcttcct agaaattatt ttctattttg aaacagctat
     5101 tttgcatttt aggtcatgtt atcatattct attgtatata attgttatat acaagtatag
     5161 gagggttata tgatactaaa agtaacaata attgggcttt tggcaggtgt tatagggaca
     5221 ggattaggag gagtaatatc agccattttt aaaagagagg tcgataaata tctaagtttt
     5281 tttatgggat tatctggtgg aataatgttg gctgtagtgg tgtttgattt aatgaaagaa
     5341 tccatggaca aaatggggat aatcaataca gttatattta cgtttgttgg agctctcata
     5401 actatgtaca taaaaacaaa attagatgtg tctggaaata tggcatctgg atatcttata
     5461 ttcataagta tattactaca taatttacca gaaggactag ccattggttc ttcttttatg
     5521 tcaactgaaa gtttaggtat tacacttgct atagttatag gtctacataa tataccagaa
     5581 gggttagcta tggcattagg cttagtttgc aataaaatga aactaagtaa agttattttg
     5641 tttactgtaa tagctggatt acctatgggt ctaggaagtt ttttaggtgt ttattttgga
     5701 ggagtattta cttctcttat aggagtattt cttgctacag caggaggtac tatgatgtat
     5761 gttgtattgg aagaaatttt tccacattca aaaagtgtgt attgtataat tggtttcttg
     5821 ttaggtacaa tgattgtaaa ttatatataa aattaaaatg aaagcaggca tacaaaagat
     5881 gaaaactatt ataagtaata ttgatataaa taatattgat tatgaggaaa taaaaataca
     5941 agcaaaactt cttagagaag gtaaaacggt tatttttcca actgagacag tatatgggtt
     6001 gggtgcaaat gcattagatg aaaatgcagt aaagaagatt tatgaagcta aaggaagacc
     6061 tagtgataat ccattgatag tccatatata tgaaaaagaa gaagtatatg acttagccaa
     6121 agatataagt gataaagcta aacttgttat agaaaagctt tggccaggac ctataacaat
     6181 tatattaaat aaaaaagata taattccata caaaactagt ggagggcttg atacagttgc
     6241 aataaggatg ccttctaatg ttattgccag ggcaataata aaagaagctg gtatacctat
     6301 tgcagctcct tctgcaaata tctcaggtcg cccatctcca acaaaagcaa aacatgtata
     6361 tgaagagatg aatggaagag tggatggaat agttttagga ggagacagta attttggatt
     6421 ggagtcaact gtattggatt taacagaaga gactcctatg atactgagac caggaagtat
     6481 aactaaagaa gttttagagg aacttcttgg agaagtaaag cttgacccat cattaagtaa
     6541 aaaagaagat aatcaaaaag ctaaagctcc tggaatgaaa tataaacatt attctccaaa
     6601 tgcagatgta tatataatat ctggaaaaag agaaaatgta gcaacaaaga ttaatgatat
     6661 gataaaatct aatagagaca aaggtctaaa gacaggtgta atgtgtatta gtagaaaccg
     6721 agagttttat gatggagaag taatcgattt aggaaactct cttgaagaag ttgcaagtaa
     6781 tctgtttgat gctcttatag aaatggacaa aaagggtgtt gatgtcatat attcagaaga
     6841 gttccccaag actggagttg gacaagcaat tatgaataga ctactgaagt cagcaggata
     6901 caaagtgata aaggcttaag agggagttgt aaaatatgaa tatacttatt gtgtgtacag
     6961 gtaatacttg tagaagccct atggctgagg ctatactaag gaaagcaatt aaagaatctg
     7021 gaagaagtat tgaagaatat tctatatcat cagcaggtat atctacagca aatggaatgg
     7081 gtgcttctga aaattctata gaagtactaa aagaaatcgg tatagactta tcaaatcata
     7141 gaagtaaagt aattactaaa aaattaattg atgaatcaga tataatacta acaatgacta
     7201 aatctcataa agaaatttta gtacaagcag ttccaaaatg caaagaaaaa gtgtatactt
     7261 ttaaagggtt tgcaaataaa aatgaggaag atatatctga cccatttggt ggaaatttgg
     7321 atatttatag aagcactatg agagaaataa tgtattctgt caacgaaata gtaaaaaaaa
     7381 tatgaaacgg aggataaaca aatgaagata ggattaggct gtgaccatgg aggatataat
     7441 ttaaaaaaag aaataataag ctatttagaa agtaaaggta tagagtgtgt agattatgga
     7501 actaataatg ctacagattc tgttgattat cctgtttatg gagaaatagt tgcaaattca
     7561 gttataaata aagaagtaga ttatggaata ttatgttgtg gtacagggat aggaatatct
     7621 ttagctgcta ataaggttcc tgggataaga tgtgcagttg tatcagatgt attttctgca
     7681 aaaatgtcaa aagcacataa tgatgcaaat atgttatcat taggcgaaag agttttagga
     7741 aaaggtctag cacttgagat agttgaagca tggattaata cggattttga aggtgataga
     7801 catgctagaa gagtaaacat gattaaatca atagaagaaa aacataataa ataataaaaa
     7861 ttacagtttt atcaggagga attatcaaaa tgagcaaagt ggtagagaca aaccatccat
     7921 taatacaaca taaattaact ttaatgagag ataaaaatac tggttctaaa gactttagag
     7981 agcttttaac tgaaattgca atgttaatgg ggtatgaaat aacaaaagat ataccattaa
     8041 aagatgtaga aatagaaact cctattcaaa aaacatcatc taaagtagtt gcaggtaaaa
     8101 aattagcaat aattccaatt ttaagagcag gtcttggaat ggtagatgga ttagtgagct
     8161 taatgccagc agcaaaagtt ggacatgtag gactatatag agacccagaa acattaaaac
     8221 cagttgaata ttattgtaag cttcctcaag acataggaga aagagatata atagtagttg
     8281 accctatgct tgcaacaggg ggttctgctg tagcagctat agatttactt aagtctaaag
     8341 gagctaaaag tataaaatta gctaatttag tagctgcacc agaaggtata gcagaagtgc
     8401 aaaaatatca tgatgatgtt gatatatatg ttgcatcagt tgacgaaaga ttaaatgaac
     8461 atggatatat aattcctggt cttggtgatg ctggagatag attgtttgga actaaataaa
     8521 aataaataac tataaatatt tattagggac tttcttatat agtgtatata attttatgta
     8581 agaaggttct tttttatata ttttttctca aaataggaat tattgaattg ataaatttct
     8641 cgaattgaga ataaaaagtt tttaccaatt aaaaaagatt gcttatttta caaggtttta
     8701 tagaatagta gtctatttta atgtggcatg ttagttgctt acgattatag gtattaagtg
     8761 tattagttaa attgagaatt gaatattaat aatattattg ggagtggtaa tacatgaaga
     8821 attgtatagt agtaagaggg gctggagatt tagcaacagc agttattcat aaattacaca
     8881 acagtggttt taaagtagtt gcgttagaaa tagaagagcc attagctata agaaggcaaa
     8941 tttctttttg tgaggctgtt tatgaaaaaa aagtaactgt tgaaggtgta gaatgtatat
     9001 tttgcaatac tgaaaaagaa atagaggaag ttttatctca acataaagta gccttgatta
     9061 tagacccaaa aggagaaaaa ttatctaaat taaatccaat cgttttggtt gatggaatac
     9121 tcgctaagaa aaacttaggt acaactaaag atatggcacc acttacaata gctttaggtc
     9181 ctggattttg tgcaggagaa gatgtagatg ttgttataga gacaatgaga ggtcataatc
     9241 taggaagaat aataactaat ggtagagcag aaaaaaatac aggagtacca ggagcaataa
     9301 aaggtttttc taaagagaga gttatgcatt cacagtatga tggcataatg gaaaatgtat
     9361 gtaagatagg tgatatagtt gaaaagggag acctactttg ctatattaat aattatgaaa
     9421 aaaaacatga agttagagca acaatatctg gagtccttag aggtttatta aaggatggag
     9481 caaaggtaaa caaaaattta aaaatactag acatagaccc tagaaaagaa gaagttaaga
     9541 atgcttttac aatatcagat aaagctagat gtatagctgg aggggtttta gaagccatac
     9601 taaacaatgg gatttatccc caaaattaat ttatattaga taaagctatt aatattttat
     9661 taagatgata taataaattt aatagattaa agagagcata tgacgggtat ttaaaattta
     9721 aatggtaaaa atgctctttt tttattggac attcaattaa catgtgtata taatataaaa
     9781 ataagtattt atttaggttg aaatctaata aatatataat accagtacat aagcaaagag
     9841 gagaaaagta aattatgaga ccatcatggg atgaatactt catggaaatt gcagaagtag
     9901 tgaaaaagcg ttcaacttgt attagaagac aagtaggagc tgtaattgta agagacaaac
     9961 aaatattaac aactggatat aatggttctc cgagaaatct tgaacactgt gaaaatattg
    10021 gatgtaagag gcaggaatta aatattccct caggagaaag gcatgaatta tgtagagcat
    10081 tacatgctga acaaaatgcg ataatacaag ctgctcataa tggtattagt gtagatgggg
    10141 caactttgta tgttactaca agaccttgtg ttttgtgtgc aaaaatgtgc ataaatgcag
    10201 gtatattgaa aattgtttat gaaggtgatt atccagatga tatgtctaca gaattattaa
    10261 aagaagcagg aatagaactc ataaaatttt agtggataaa atttatatga caataaatgt
    10321 caaattatga cactgaaata caatggtatc aaatgattaa aagaaagtat ttgaaaaatc
    10381 atcagattag tagtaagata tattttataa ttaaataaat ttatgagata gattaattac
    10441 atgttattta ttttataata atgagggata ctatttatga gtaaaagaaa tacacataca
    10501 gaaattgcaa agttgtttag tttaataagt caaataggat tgatgattgt aatatcaata
    10561 ttaggatgta ctttcttagg aaaattttta gatagtaaat tgaatactac tccagttctg
    10621 acaataatat ttttattgtt gggtgttgga ggtgctttta gtggtgttta taaaactcta
    10681 atagtatata cacgaaggaa gtgatatatt ggatataaaa ttacttaacc aggtaaggtc
    10741 agtaacaaaa ggtatagtaa tatttgatgt agttttaatt gtgctaatgt tgataacatc
    10801 aacattaagt aaaccagtat tattaggact tatatttggc tctataatag ctatgttaaa
    10861 ttttagatta ttggcgatat cactggaaaa atcagtgaca tttcctgctg ggaaggcaca
    10921 agcttatgca tcagcacaat atgctgttag attatttata atggcagttg ttctaattgc
    10981 atcagctaca gtaccacatt taaatataat cggaacagca ttgggattat tgagcacaaa
    11041 gtttgtcata ttatcaaaaa actttataaa taaattaaag aggaaggagg cataagtatg
    11101 aatcaagcgc attattatct cataggaaac tttaagatta gtgaaacaat actagtaagt
    11161 tggggaataa tggccttttt agcgatagca tcttattttt tgactagaaa cttaaaaaaa
    11221 gttccaacta gtaaagttca aatattctta gaatttgcag taggtggtct tgctaaaatg
    11281 gttacagatg ttatgggtaa aaatacagta aaaagaatgc catatatgat accatacata
    11341 ggaagcttgt ttttattttt cgcatgttca aatttaagcc cattacttgg attaaggtca
    11401 ccaacaaccg acttagacac aactgctgca tgggcaatga ttacattttt tatgatttat
    11461 tttgcaggag ttaaatttaa ggggttatca tactttaaag aattggctca accacttgcc
    11521 ataatgttac caatcaatat tataggtgag ttagctagac caatatcatt aagtttccgt
    11581 ccatttggga atattttagg tggaacaata ataatgggtc tgttttatca acttttaggt
    11641 tttatatcta gtttaatacc aggattatca ataccaattg gagaaatatt aataccagtc
    11701 ccattacact tgtactttga cttatttgca ggattgttac aagcatatat atttataatg
    11761 ttaacaatgg tatttatatc aagtgcagga gagttagatt aagaaatcaa cttatattaa
    11821 gataaaaata aaataattta aaaaattaaa gttttaagga ggaaataatt atggaaacag
    11881 caatagtagc ggcagcatca gcaataggag cagggatagc agttgcaaca ggtataggtg
    11941 caggtatagg tcaaggtata gcagcagcta aagctgcaga agcagtagga aaccaaccag
    12001 aagctaaagg tgatataaca tcaactttat tactaggagt tgctatagca gagtcttcag
    12061 caatatatgg tttggttata tcaataatac ttctattcgt aaatccattc tttaaatact
    12121 taggaatgta gatttagtcc ttaaatattt tgagataggt ttataggtaa atcatctcaa
    12181 acttataatc atgagatgaa ctttatcatc tcgtgattat aagtagttat gatttatgga
    12241 aaggaggata attatggaaa aagcactagt aggtataacg tgggaatttg tgttccaaat
    12301 tgttaatact tttataatat ttttacttct gagaaagctt ttattcaagc cagtattaaa
    12361 tataattgaa tcaagagaaa atgatattaa aagtgatttg gcagaaggcg aaaaagcaaa
    12421 aaatgaaggt ttagctctga aaaaagaata tgagtcaaaa atcaattttg ccaaggatga
    12481 aggtcaagaa atcataaaac aagcaactat aagagcagag cagaaatcag atgatatagt
    12541 aaatactgca aagaaagatg ctcttgatat aaaagaaaaa gcaaacaaag atatagaaca
    12601 agaaagacaa aaggttataa atgaaatcaa aaatgatata tctaacatag cactacttgc
    12661 agcatctaaa gtcattgaaa aagatttaga taaatctaaa catgaagaat tgatagaaaa
    12721 ctttataaaa gaggtagggg aagctaaatg ataaatgtaa tagcaaatag atatgcagaa
    12781 gccctatttc aattaggaga agaagaaaat tcaactgatg tcttattcaa agaactagaa
    12841 aaagtagtag atatgatgac taaagttagt aaagatttct acaaagtttt aaaatcacca
    12901 ttagtttcaa aatcagaaaa gaaaaacttg gtagaaatta tttttagtaa agaagtaagt
    12961 tctaacataa aaaacttttt aaaagtttta gtagataaag atagaatatc atatttagaa
    13021 gatatcgaat tagcttataa agaattatta aataagaaaa acaatgtaat tgatggagta
    13081 gcaataagtg ctattccaat gagtgaaaca gatattaaag aacttgaagt caaactttca
    13141 aataaataca acaaaaatgt tactatagaa aatgttgttg acaagactat actaggggga
    13201 gttctagtta gaattggaaa tgaacaaata gatggcactg tgaaaactcg tcttgataag
    13261 atgaaagaaa agctatctga agtaatatct taagaatggc ggtgaaccca tgaacttaaa
    13321 acctgaagaa ataagttcta taattaaaca gcaaataaaa aattatgaga ataaagttga
    13381 gttgacagat acaggtagtg ttttaacagt tggggatggt atagcgagtg tatatggatt
    13441 agaaaaagct atgtctggtg agttgctaga gttcccaggt gaaatatatg gtatggcact
    13501 taacttggaa gaagaagtag ttggagctgt tatccttgga gatgacagtg aaataaaaga
    13561 aggcgatata gttaagagaa ctggaagaat agttgaagtt ccagttggag aagctttaat
    13621 aggtagagtt gtaaactcac taggtcagcc aatagatgga aaaggaccta tagcgtacac
    13681 aaaaactaga ccagttgaat ctgaggcacc tggtataata gatagaaggt ctgtttatga
    13741 accattacaa acaggaatca aatcaataga ttcaatgata ccaattggta gaggtcaaag
    13801 agagttaata ataggtgaca gacaaactgg taaaacatct atagttattg acactatact
    13861 aaatcaaaaa ggaaaagatg ttatctgtat atatgtagca attggacaaa aacgttctac
    13921 tatagctcaa ttagttagct ctttagaaaa aggtggagct ttggattata caatagtagt
    13981 ttcagcaaca gcatctgaat cagcaccact tcaatatata gcaccatatg ctggagcagc
    14041 tatgggtgaa gaatttatgt acaatgggaa acatgtactt atagtgtatg atgatttaag
    14101 taaacaagct gttgcatata gagaaatgtc attgttactt agaagaccac caggacgtga
    14161 ggcatatcct ggagatgtat tctattttta cttgaaagag cagctaagtt atctgatgaa
    14221 ttgggtggag gttcaatgac tgccttgcct ataatagaaa ctcaagctgg tgacgtttct
    14281 gcatatatac caacaaatgt tatatctata acagatggtc aaatatactt acaaccagag
    14341 ttattctatt caggtgtaag accagcagtt gaccctggta tatcagtatc aagggttggt
    14401 ggttctgcgc aaattaaagc aatgaaaaaa gttgcaggaa cattaaaact tgcatattca
    14461 caatatagag aacttgcagc attctcacag tttggttctg acttagatga agatactaaa
    14521 aagagacttg ctcaaggtga aagaatcgtt gagatattaa aacaaggtga gcatcaacca
    14581 ataaaagttg aaaatcaagt tatgataata tatgctgtta taaataatca tttagaggat
    14641 attccgatag ataatatagc tagatttgaa tcagaattgt atgcatttgt agataataat
    14701 tatccggaaa tatcaagaaa aatattaggt ggagaagact tcactcatga tttaacagat
    14761 gcaataaatg aatttaaaga aaagtttgtt gttgaagtat aatccttttt aaaaaaggag
    14821 gtaaattaat tggcaggagc tggaatcaaa gcaatcaaaa cacgtatggg aactgttgaa
    14881 agtacaaagc aaataactaa agcaatggaa ttagttgcat cttctaagct tagaaaagca
    14941 aaagaaaagg ctgaagattc aagagaatat tttaatactt tatacaatac agtaaaagat
    15001 attgcccaaa caacacgtgg aattagaaat gaatttttaa aagaaagaga agtaaaaaat
    15061 aaatgctatg tagtagtagc tggagacaga ggacttgcag gtggatacaa ttctaatgta
    15121 ttaaaggaat ctgtctctca tatgaaaggt aaaaaggaaa cagtgattac tgttggaaag
    15181 aaagcacatg aatttttttc taaaagaaac tacaatatag caaaagatat aaatagtgtt
    15241 gaagcttgtg gatatgaaga tgcgacagaa atttcacaaa ctattattga cctgtataaa
    15301 aaaggtgaag tagatgaagt ttatattgct tatacaaaat ttaaatctgc tttatcacaa
    15361 gaagttgaaa ttttgaaatt gcttccatta gtctttgaaa aagatacaga cgaacaagga
    15421 atggcagtaa aagaagaaaa acaaaataga tcaaaagtac aatatatacc atcagcagaa
    15481 gcagttttaa gttatattgt acctcagttt atatcaggta cagtatatgg tggaattttg
    15541 gaatcttttg catcagagca aggtgctaga agaactgcaa tggaatcagc cacagacaat
    15601 gcgaatgaaa tgctatctaa tttagagtta agttataaca gagcaagaca gtcagctgta
    15661 actcaagaaa taacagagat agttggtgga gttgaagctc taaaataggg aggttaggaa
    15721 atggcaaatg taggtaaagt agtacagatt gtaggagctg tactagatgt taagtttgat
    15781 agtgaacaaa gcctacctaa cttattaaat gcgttagtaa taaagttagg agataaagaa
    15841 atagttgcag aagttgctca acatataggt gatgatactg taagatgtat agctatgagt
    15901 gcaacagacg gacttgtaag aggtatggaa gttgtagata caggagggcc aataagtgtt
    15961 cctgttggag atgaaacttt aggtagaata ttcaatgttc ttggaaaacc agttgatggg
    16021 aaaccagctc caaaatctgc acctaaatta cctatacata gaccagcacc agcatatgat
    16081 gaactagaaa ctacagcaga gatactagaa actggtatta aggtagttga cttactagct
    16141 ccatatctaa aaggtggaaa aataggtcta tttggaggag caggagtagg aaagacagtt
    16201 ctaatacaag agcttataaa caatattgct aagcaacatg gtggtatttc tgtgttttca
    16261 ggagtaggag aaagaacaag agaaggtaac gacctttatg gcgaaatgag tgagtctgga
    16321 gttataaata aaacagctct agtatttggt caaatgaatg aaccacctgg agcgagaatg
    16381 agagttgctt taactggact tacaatggca gaacatttta gagatgagca aggacaagac
    16441 gttttacttt tcgttgataa tatattccgt ttcacacaag ctggttcaga agtttcagca
    16501 cttctaggac gtatgccatc agctgttggt tatcagccaa cattagctac tgaaatgggt
    16561 gcacttcaag agagaataac atcaactaag aaaggttcaa taacatctgt tcaagcagta
    16621 tatgtaccag cagatgactt aactgaccca gcaccagcta caacattctc tcacttagat
    16681 gcaaaaacag ttttatcaag acaaatatca tcactgggta tatatccagc agttgaccct
    16741 cttgaatcta catcaaggat tcttgaccct agtatagtag gaaaagaaca ttatgaagta
    16801 gctagaggtg tacaatcaat acttcaaaga tataaagaac ttcaagatat aattgctata
    16861 cttggtatgg atgaattatc tgatgaagat aagttaatag ttgcacgtgc tagaaagata
    16921 caaagattct tatctcaatc attcacagtt gctgagcaat ttacaggaaa tccaggtcaa
    16981 tatgttcctg ttaaagagac tgtaagagga tttaaagaaa ttcttgaagg gaaacatgat
    17041 gacttaccag agtcagcatt cttatttgta ggaacaatag aagacgctgt tagaaaagcg
    17101 aagggaagta tgtagtcatg gccagtgaat tttcattgaa agttgtaact ccaagtaata
    17161 ctttttatga tggagatgtt gaaatgatag tagttagaac tacaaaagga gacaaagggg
    17221 tgttgaaagg tcatatacca tttgtttcag gtatacctgt aggaacttta aagataaaaa
    17281 aagagggaag ttttaaagag gcgaaaatag cagaaggatt cattaatgta actcaagata
    17341 aaactgtcat attgacagaa tctgctgagt ggatttaata aattaattta gagtataaaa
    17401 agtcattttg accaaaaaaa cttttgggta aaatggcttt tttgcgtttt ggtgttaata
    17461 aaataacgat aaaattgtaa gtttttagtg ataataaatt cacaaaatgt ttaaatttac
    17521 tgccaaatta tgttgtaatt aataagataa agaagattta gatagctttt atgtaagtga
    17581 aaaagtcttc atataagaaa tacatataaa atttttataa aatttaaaaa atttgct
//
LOCUS       DAELTA010000081        17308 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.081, whole genome shotgun sequence.
ACCESSION   DAELTA010000081 DAELTA010000000
VERSION     DAELTA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.081"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            95..2158
                     /locus_tag="KPG13_002232"
     CDS             95..2158
                     /locus_tag="KPG13_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0985946.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2155..4020
                     /locus_tag="KPG13_002233"
     CDS             2155..4020
                     /locus_tag="KPG13_002233"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG0985947.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4144..4958
                     /locus_tag="KPG13_002234"
                     /pseudo
     CDS             4144..4958
                     /locus_tag="KPG13_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4983..5471
                     /locus_tag="KPG13_002235"
     CDS             4983..5471
                     /locus_tag="KPG13_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG0985948.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5584..5745
                     /locus_tag="KPG13_002236"
     CDS             5584..5745
                     /locus_tag="KPG13_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985949.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            5985..6212
                     /locus_tag="KPG13_002237"
     CDS             5985..6212
                     /locus_tag="KPG13_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0985950.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6209..9148
                     /locus_tag="KPG13_002238"
     CDS             6209..9148
                     /locus_tag="KPG13_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG0985951.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9148..9822
                     /locus_tag="KPG13_002239"
     CDS             9148..9822
                     /locus_tag="KPG13_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985952.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            9983..10399
                     /locus_tag="KPG13_002240"
                     /pseudo
     CDS             9983..10399
                     /locus_tag="KPG13_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10359..12806
                     /locus_tag="KPG13_002241"
                     /pseudo
     CDS             10359..12806
                     /locus_tag="KPG13_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12946..13368
                     /locus_tag="KPG13_002242"
     CDS             12946..13368
                     /locus_tag="KPG13_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985953.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13906..14940
                     /locus_tag="KPG13_002243"
     CDS             13906..14940
                     /locus_tag="KPG13_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG0985954.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15027..15230
                     /locus_tag="KPG13_002244"
     CDS             15027..15230
                     /locus_tag="KPG13_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG0985955.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15401..16561
                     /locus_tag="KPG13_002245"
     CDS             15401..16561
                     /locus_tag="KPG13_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG0985956.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16820..16960
                     /locus_tag="KPG13_002246"
     CDS             16820..16960
                     /locus_tag="KPG13_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0985957.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7086 a         1678 c         3003 g         5541 t
ORIGIN      
        1 tattttagca taagatgtat aataaaggaa aaaatacaga attaaaaaac ataagataga
       61 taagtggact taggggagag aaattattat gcaattgaca aaaataattc ttagtaacta
      121 taggtgtttt ggagaagaac ctacaactat tgaaattaat aatttaactg gatttatagg
      181 tcataatagc agtggtaaaa ctgctttatt aagtgcactt atgaaacttt ttggagaaaa
      241 gtctagtgat agaattattg aaagatcaga tttccatata cctgaaagta ttgatccaga
      301 tagtatttct aagaatgaat tatatattga agcacaattt gagtttcctg aattggagag
      361 aggtaaagac agcatatcta taccagtatt ttttaaaaat atggttgtta gtgaaccagg
      421 tgggaaacct tatttaagaa ttagacttga agcaaattgg gagaaaagct caaatcctga
      481 aggaagtatt gattcaaatg tatactatat aacatctaca acagaggaga taaatcctga
      541 agataagcgt aatatcaaaa gatatgagct tgatcaaata aagatgattt atgtacctgc
      601 cattaggaga ccagatgaac agttaaaaaa tgtctcagga acaataattt caagattatt
      661 aaatggtata agttggtcag aacaaacaaa ggcaagaatt aaaacgaaag caagtgaaac
      721 ggaggaaatt ttctttgaag aaaaaggggt tgaatcatta aagaaatcac tcgaaactca
      781 atggaatagt tatcataagg atattagata ttctaatcca aagataaact tcaatagtac
      841 tgatttagag acaattttaa aaaaagttga agtaactttt tcacctacag ggacacatcg
      901 agattatcgg gttgaagagc ttggagatgg gttacgttct ttattttatc tttcacttgt
      961 aaatacattt ttagaaattg aagaagaaat tctaaaatct attaatgatg gtaaagaaga
     1021 agatatattt acattaatac cgcctgtatt aacaattgtt gcggttgaag aaccagaaaa
     1081 tcatatttca cctcatcttt taggaagggt agttgaaaaa ttaaatttta tttctacaaa
     1141 agaaaattca cagacaatat tgacatcaca ttcaccagca atagtaaaaa gaattgaacc
     1201 aacagacata aggtatttta ggatttgtaa ggaatatgaa tgtacaaaag tgaagtcaat
     1261 tatattacca aatgaagaaa aggaagcaga acaatttaaa tttgtaaagg aatcagtatt
     1321 agcatatcct gaactttatt ttgcaaaact tgttgtattg ggcgaaggtg atagtgaaga
     1381 gatcattatt aagcagatga ttgaattaaa agcagaatct gtagattcat cagaaatttc
     1441 aatagtacca ttaggtggta ggcatgttaa tcatttttgg cgtttattaa atgatttaga
     1501 aattccattt attactttgt tagatttaga tagagaacga gatggaggtg gttggggacg
     1561 tataaagtat gcaatacaac aattaataaa tattggtcat gataagaatg tattacttta
     1621 tacatgtgaa ggaatttcac aagataatga atttaaagag atgcatactt gggatgtaac
     1681 tgatactgaa aatatggcta aatggattga aatgctaaaa ggttatgatg tttatttttc
     1741 agctccgtta gatatagatt ttgcaatgtt acaaagtttt aaagataaat atatttcagc
     1801 attaacaagt gcggaaggtc ctaggataaa agatcatgga aaaatacaag atattgtgat
     1861 caatgaagat gctgaagatg aatcattgaa agctgcttat aaagatagaa tagatagtga
     1921 tattaaatca accttaaaac aagaaggtgg tgatggatct acttatactg agggagagaa
     1981 agaactaatg atatggtatt catatttctt tttaggaaga ggtaaaccaa ctactcattt
     2041 atctttattt tctaataata ctgatataga ttttgaaagt tttccatact gtctaaagga
     2101 gtttgtaaat agtattattg aaaagatgat agatgattca gggggtggaa gaaaatgaag
     2161 aggaattggg ttagtaaaga agattggagt ccagcggctg gattcagact agaagttgaa
     2221 gcgatgaata cagttattaa tgataagaat acacttgttg tagctggtcc tggcgctgga
     2281 aaaacagagt tattagctca gagagcctgc tttttactag agactaatac ctgtagatat
     2341 cctaaaaaaa tccttgctat tagttttaaa aaggatgctg ctgaaaattt aaaagagcgt
     2401 ttagaattga gatgtgggaa agaattagct tcaagatttg aatcaaagac ctatgatgct
     2461 tttgcaaaag aaatcgtaga tagatttaga aatagtttag atgaagcata tagaccttta
     2521 aataattaca atatagctga aaatcgtgat gttagaagag catttgagat agcaggatta
     2581 aatttcagag gaaatcaaac tgaatttaat aaagtatatg gaaataaact atcgataaat
     2641 aaattgcctt tacatacaga ttttgtagat aagattttta tagatgcttg gaatgtacta
     2701 cttaaaggca acaaagatac taattttgta agttcactta cttttggtat gatatcacgt
     2761 ttagccgaat atttgataag aaccaatagg tatattaaga aagcaattga aatgacttat
     2821 agtcatgtgt ttttagatga gtttcaagat actaaatcta ttcaatatga tcttgttaaa
     2881 gcttgttttt ttaaaacaga aacaataatt actgctgtag gggatggcaa acaacgaatt
     2941 atgttatggg ctggtgctag aaagtcaata tttgaagatt ttcaaaatga ttttttaagt
     3001 caaaaaagaa cattaattat gaatcatcgt tcagcgccaa gacttgtaga gattcaaaaa
     3061 gcaatgtata acagtttgaa tgaagaggca attgatttaa aaactaatga aaagtggaat
     3121 tatgaagatg gagaagtgta tttaagactt tttgaggatt atataaaaga agctgatata
     3181 cttagtgagg aaatagtcga attggtggaa acaggtatag aaattaatga aatatgtata
     3241 ttagtaaaac agagcgtaga tatttattca aaagagatta taaataaact ttctgaacgt
     3301 aatattaagg cgcgtaatga agcaatgtat caggagatat taaaagaaga tataataaag
     3361 acactggttt cgctttttag gttgtcgtta agtgatagaa atcccgatga ttgggattat
     3421 atttacgata ttctaggtga attatatgga atagacgaag ggtataaccc taaaatatta
     3481 aatgatttca ttaccaaagt agaattaatg attaatgatc tcaaggtaaa tttaaatatt
     3541 actgatgaaa aacaattttc agatttagtt gattctaagg ttgaatatat ttcatatgaa
     3601 aaatttgcat caaagtttca aaaatataaa tctaaagatt attttgatga tttggtagat
     3661 aaatttaaaa agctggtatg gattgaatat aatgaaacta aagattggat taaagcgatt
     3721 gataactttg aaggtaaaaa ttcaattcca ataatgacaa ttcataaaag taaaggactt
     3781 gaatatgata ctatcttttt tgttgggtta gaagattcag cattttggaa ttttaaaaat
     3841 caaccagatg aagatagatg tgcatttttt gttgcattat caagagctaa aagaagaatt
     3901 gattttacat ttagcttaga tagaccagca ggattttcga attcacagaa gcattattgt
     3961 attaatgaat tctacgaatt gtttgataat actgagattg taaaaaagat agagtattga
     4021 ttaagatgat aaaaacttta gtatattata ttttaagata ttagttatat aagtagacat
     4081 tgaatttatg tatttgatgt ctatttttta tataaaaata tattaaagaa aggatgaatt
     4141 tgcatgaaga aaaaaacaaa aatatctaaa aagattgtaa gttttattat ggtttttata
     4201 actatagtta ctactatact tacatcatca gtttcagctt atgctagcga gttggatatg
     4261 agtgaacaga caggctttaa ttatttaggt attagtccaa ttacaggaaa aaaattaatc
     4321 ataacattta tcgtatgaag atggataata aaattgtatt ttgtgtagag ccaggaatta
     4381 aaacttcaag tggaggagga tatatatcgg aggagttagg agataaattt atatctacaa
     4441 cacttccaaa ttatcataaa agaaaagctg aaatacttga gaaggtaaat agacataata
     4501 tattaccttc atggaataat gaaaaagttg cagttaaaag tggagaaaca attgagttag
     4561 aagataagaa tggtattatt agagaaatgc aattagaatc aaataatact ggaacagata
     4621 tattacttga agaaaatact ttgaaaatta cagcaaataa aaattctaat tcaggagtta
     4681 ttagttatat aaagattcct aaaaatgagg ttggaatatc aatagtttat cgtaagcata
     4741 actatcaaag cttatcagaa tttcatatgg aaagttcgat gagtgcaaat ttaaatataa
     4801 atgtaatcaa gttaggaatt gttaaagtaa aaaagattga tgaaggcaca ggaaaggaat
     4861 tagcaaatgc aaaacttaga tttgagtatg atggtgatag taaagaaatt ataacgggtt
     4921 ctaatgaaat tgctagtata gaagatatta ttggttgatt aaaaagtgaa gtatactttt
     4981 gtatctatga aaaagactat aagcaatata taaaattaga tatacctatt gatgaagcaa
     5041 aaataaaaaa tagatttgaa ataaggttaa aaaatgaaag agcatatcat gcagcagttg
     5101 atttgttagc aaatagagat gaagaaaaga cagcattttc aattattaat cgttatatac
     5161 gttttgttga taaagatgat gaaaaaatta gaagtgaatg gaaaactaat gatcagtgga
     5221 gttattttat tggtgaaggt agagcgaagt taaaactaac aacaaatcca gaaccatata
     5281 atattcaaaa aactttaaat tggttatcaa gacaagttgc accaacgtta aaaacaatta
     5341 aaaggttaga tagtttgaac aactcaacat taattgaggg cttaataagg tatgcaagac
     5401 taacagataa acataaaaaa ataattgaac aaaattcagt aaatgttgaa gacattattt
     5461 atgatgaata aaaaaatgta attagtattg cagattttaa tataaaagaa gggagatata
     5521 cgaatgaatt ttggtcaaaa tttatataat tagtttttat caaatgcaca atcattggta
     5581 ttaatggcta tagctataat tggtgtatat ttaggattta agagagagtt ttcaaaactt
     5641 ataggatttt tagtagttgc attaattgca gtagggcttg tgtttaatac agcaggagtt
     5701 aaagatatat tattggagct atttaataaa ataataggtg tgtaaggatt aaaagagaat
     5761 agaaaattat gagtatatga tgaagggttc taataagttt tattaaaata tatattgaaa
     5821 gacaagtaga gctatttcga gttattacat gtaatgctaa tatatataac tgcattttgg
     5881 aaattaaata cataattaga aatagcaaat tttataggtg acttattaat atgtttactt
     5941 attgtatact atttgatata tttaatatga ggtgattctt gaaaatggac atagtgaaag
     6001 tttttgctaa caatgtgaga aaatatagaa ataaaaaagg tctatcacaa gaagcttttg
     6061 ctgaaaaagc tggattgcat agaacttata tttgtgctat tgaaagaaaa aagagaagta
     6121 ttgctttaga taatgtgcag aaaattgctg atgcgcttga gattgagact tacttactat
     6181 ttatagaagg tgaggagaaa gaatgtctat gaaaaaatta ccaatagaaa agcttataga
     6241 aaatttaggt ttttcagggt caaagaatct tgtgaatata gatatgctta caaaattaga
     6301 tatatctaaa catgatgtaa aaactttaaa agttataaat ccttatgcaa tttatttagt
     6361 aaatagtaag ccatttatat tatttataga gtcttctttt gaagaaaaaa aattacaaca
     6421 aattagtaag cttatatgga atgctcaaat accaattgct tttatatgcg attctaattc
     6481 tgttaaaata tttaatggaa aagctttgga tttaaagtca tatttaattg aggaaatata
     6541 taatcaatct attgaggatt tttcggaaga atctgagttt tcttatttga aaatatctga
     6601 tccattattt tgggatcaat acactaaaca atataccaaa acgcatttaa atgattattt
     6661 gctagataat attacttcat tgacaaataa attaaagaaa aaatataagg tgaaatttgc
     6721 aacgaagctt gttctaagat taatatttat tagatattta atcgatagag gtgtcgatat
     6781 agcttataaa aattttaatc atgatattaa agcttcacaa aaagaactgc taaagattac
     6841 taaaaacaaa gttgaattat atgaactgtt taaatactta aaagaaaagt ttaatggtaa
     6901 tttatttgag ttaaatgacg aaattaataa tacttgttta acagatgaag tgtttatttt
     6961 actatctgat tttttatcag gtaaaataag tattgacaat ggacaaatgt ctttattttc
     7021 aatgtatgac tttaatatca taccagttga actaattagt aatatatacg aaattctttt
     7081 aggaaaagaa attcgtaata aagataacgc attctatact ccgaattact tagcagaata
     7141 tattttagat aaaacaacac ttgaatttct gaaaaataat aggcaataca aaattctaga
     7201 tccttcttgt ggatcaggag tatttttagt aaatagtttt agacgtatta ttgatatgaa
     7261 tttgggtgat aaactttttt gtgaagatga taaactttta aaagaacttt tattatctaa
     7321 tatttatgga attgatatca atgaagatgc tatcgatgtt actattttct cgttatatct
     7381 aactatttta gattacaaag accctaagat attatcagaa tttaaactgc ctaatttaaa
     7441 agggtgtaat ttaatagcga gtgatttttt tgatgataat aaattggaga gtttaaagaa
     7501 aattaacttt gattttatca ttggaaatcc gccttgggga agtgtctcag atggattaca
     7561 tttagattat tgcaataaaa atggctataa tgatatgcaa caaaacaaag aaatcagtag
     7621 aagttttatt tttagagcaa aagattttag ctctagaaat acagtatgtt cttttgtagt
     7681 gcattctaaa atactatata ctcaaaaaaa gccatcaaaa aaattccgtg aatttttgct
     7741 taaaaaaagt caaatattta gtgtagttga aatgtcatct gtaagaaaat tagtgtttaa
     7801 aaatgcagac gctcctgcag cagttatatc atttaagtat aacgaaagtg aagaaaattt
     7861 aaataataga atcatctata catctttaaa accaaatgta ttttttaaat tatttaacat
     7921 tattgtaatt gaaaaatatg atgttaaata tatttcacaa aaactattat tggagaatga
     7981 ttgggcttgg aaaactattg tgtatggatt cagtaaagat atagatataa ttatgaatat
     8041 caaaaaaaaa tatcaaactg taaatgaaat tattttgaaa aacagttttg ttcatggaac
     8101 agggatacaa atcaatggta atgaaagcca tgatgcgaca catttaaaag gaaagtggta
     8161 tgttgattca aagaaaggaa tagattcatt ctatgttgat ataaataaag ggaaagaact
     8221 tactcaagat aaaatgagaa gacctaaggc taatcagcaa gaaatattta ttcctccata
     8281 tgtattatta aaaaagggag ttgacacgaa aacatataga tatagagcag cattttcaaa
     8341 tgatgcattt gtatattcag atgcagttac aggaatatgt ggaaaaaaag ataatttaac
     8401 tacactttat tgcttgactg gtatgcttaa ctcttctttt tactcatact taaatataat
     8461 gctaggtaca tcaattggaa tagaaagaga acaaggattt tcaacggaaa tttttacgta
     8521 tccagcaata gaaagtgatg aaattgctaa tctggtagta aaaatacaag aagcgactaa
     8581 attagaaaaa acaactatgt tcttgaatga aaaaaatagt tgtaagtata tcaaacaatt
     8641 agataagtta attttgaagt cttttaattt aaatgatgat aaatttattg actatgcaat
     8701 aaatattcaa attcctgagc ttacaaattc taagtcaact aacatatata gaaaaataac
     8761 taaggaagat ttaattaatt atagtgaatg tttcaaagaa cagttttcat cgatatatcg
     8821 tagggttggg aaaagtattt caatttcggt atatccaaaa atattaaatc gttttacagt
     8881 atttgaacta gaagtaacag atgaaaaaac agatagagag attatggtta aagcagaagc
     8941 tgataataat atggagttgt ttacgaaaat aattagatat gattataatg atgtttttca
     9001 tcaaattaga gatattattc attttggaga aaattcattt tatatcataa agcctaattg
     9061 ctataaatat tggcatcctg ctatcgcaga aatggatctt gctgatgttt tggatcagat
     9121 tttatctgat acagggggtg aagaataatg ggaggattta aaaattcttc actaaacttt
     9181 tatttaaagc aagggcttta tgaaataatt atagatggat taatagactg ttgtttactt
     9241 atgagcaaat catgtattga acaatctaga aaaatattaa atcacgaaga aaagataaga
     9301 acgcatttag ttgaaaatta tttagaaaat gatattgaaa gaagaaaaac atctttatct
     9361 aaacaacatc taagatttga agtagaagca caagaaaatt atgacaacaa aaatgaatct
     9421 tatgagggta gggttgatat aaaagttata tctaaagaca ctttagcgga tagaagggct
     9481 tattatatca ttgaatgtaa aaggatagat ggtaaggaaa ctttaaataa aaaatatata
     9541 accgaaggtg tacaacgatt tattactgag aaatattctt cgcattatgg caaaaatata
     9601 atgcttggat ttatagtaaa agatataaat atccagagca atatacaaaa aattaattca
     9661 ctgaatcaga acatattata tacatatata gatacagata tgttgacagt tagaagtgat
     9721 tctgaaaata attatgctgt ttgtgaaagt aaatataaag taaaatctaa actactattg
     9781 ttaaatcata ttttttataa cttttcgagt ataataagat aatcttatga atataaaata
     9841 ttactatgtt aaaattatag tttggttttg ataatattat aaaaataagt ttaagaataa
     9901 aatataaagg aaaaaagggg cgtagcacta ttttttagtg tgacagcccc tttaatttta
     9961 aaatcggtaa ggaggagtgt atttgaaaaa aattaagtca tatacaggga ttttgaatgt
    10021 ggagaaggtt atatatgcaa taaatgattt tcagcttcca tttccagtta cttttacgca
    10081 gatgacatgg tttgtattaa cactttttat aattatatta tttggtaagt tacctccatt
    10141 atttatgata gaaggagcat ttttaaagta tcttggcata ccaattttta taacttagtt
    10201 tatgtctcaa aagacttttg atggaaagaa accatatgga tttttaaagt caataatact
    10261 atattcatta agacctaaaa taacatacgc aggaaaaaac attaaattta aaaataaaat
    10321 tattgatgaa aatataacta tagtaaggag tgaattatat gttcccgata aaatatatag
    10381 agaacaatct tgtatttaat aaggaaggag aatgttttgc atattatgaa ttaataccat
    10441 ataattattc attcttataa gctgaacaaa aatttattgt acatgataat tttaggcaat
    10501 taatatcaca aagtagaaat ggtaaaattc atgcacttca aatagccact gaaagttcta
    10561 taagagttac acaagaaagg ttaaagaaaa atattacagg aaaattaaaa gaaattgctt
    10621 atgaaaaaat agacgagcaa acagaggcac ttgtttctat gattggcgat aatcaaattg
    10681 attatcgttt ttttattggg tttaagcttt tagttacaga gaaagaaatg aatataaaga
    10741 gtataaaaaa gtcaatatta gctaatctat cagattttat atatgatgtt aataataaat
    10801 tgatggatga ctttgtcagt ataaataatg atgaaattat gagattttta aaaatggaaa
    10861 agttattaga aaataaaatc tcaagaagat ttaaagttag aaggttagat aaaaatgatt
    10921 ttggatattt aattgaacat aattatggaa agaaaggcac agtttttgaa gattatcaat
    10981 accatttacc tacaaagaaa cttaaaagag aaacattagt aaagaaatac aatcttataa
    11041 agccaactag atgtcttata gaagagaagc aaagatactt aaaaattata catgaggatt
    11101 cagaaagttt tgtagcttat tttactattg aaattattat tggagaaatg aattttccat
    11161 ctagtgagat attctattat cagcaacaac agtttgcatt tcctattgat actagtatga
    11221 atgtggaaat tgtaacaaat aaaaaggctt taactacagt tagaaataag aaaaaagaat
    11281 taaaagattt agacaatcat gcttggcagt cagggaatga aacagtaaca aatgtagtag
    11341 aagcattaga ggctgtaaat gagcttgtaa ataatttaaa tcaaacaaaa gaatcaatgt
    11401 ataagttaag ctatgtaatt agggtatcag cagatagcat tgatgaatta aaacgccgtt
    11461 gtgatgaagt taaagatttt tatgacgatt taaatataaa attagtacgc ctaatgggag
    11521 atatgatagg atttcacaat gaatttatac cttcaagcaa aaggtatata aatgattata
    11581 ttcaatatgt aactagtgat tttctagcag gattagggtt tggagcaact caatagcttg
    11641 gagagaacga aggaatatat attggatata gtttagatac aggaagaaat gtctatcttc
    11701 aaccatctct tgcaagtcaa ggagtaaaag aatctgtaac taatgcgttg gtagcagcat
    11761 tctttggatt tttaggaggt gggaaatcat ttgctaataa cttacttgtt tactattcag
    11821 tattgtttgg agggcaagct gttttggtgg atccaaaagc tgaacgtggc aaatggaaag
    11881 aaaatttacc agatatatat catgaagtaa atattgttaa tttaacaagt gatatagaaa
    11941 ataagggaat gctagatcct tatgtaatta tgaaagaagt aaaagatagt ggaagtctag
    12001 ctattgatat attaactttt cttacaggaa taagtagtca tgatggagag cagtttccta
    12061 tacttagaaa atcaatcata aatgttactc aagacaaatg cagaggactt ttgaaggtaa
    12121 tagatgaatt aaggaaagat aattctcaaa tatcaaataa tattgcagat catattgaaa
    12181 gttttacaga ttatgatttt gcacaattac ttttctcaga tggaaatatt aaaggctcaa
    12241 ttaatctaga aaaacaaatg aatataatac aagttgcaga tcttgtttta ccgaataaag
    12301 atactttatt tgaagaatat acaacaatgg aaagtctttc agtagctatg ttgatagtaa
    12361 ttagtacttt tgcattagat tttatccata cagatagaag tacttttaaa atagtagatt
    12421 tagatgaagc ttggacattt cttaatgtag cacaaggaaa aactttatct aataaattag
    12481 ttagagcagg atgtgctatg aatgcaggag tgtattttgt aactcaaaat gcaggtgatg
    12541 tatcagatga aaatttaaaa aacaatattg gattaaaatt tgcattcaga agcacagata
    12601 tcaatgaaat taagagaata cttgaatttt ttggagttga taaggaagat gagaataatc
    12661 aaaaaaggtt aagagagtta gagaatggac agtgtttaat acaagattta tatggacgag
    12721 ttggggtaat acaaatacat ctagtttttc aagaactcct tactgcattt gatacaagac
    12781 cacctataag aaaagaggtg gattaatgaa atcgaaagta tttagaaatt taagctggat
    12841 aactatttta atagtagtac ttttactatt ttttatttat ataaatttat agatttagag
    12901 aaacaaatag atacattaga taagaaaaga aaaaaactca tagatatgag attagaagat
    12961 ataattgata aagaatcata tgaagaaaag tatgatgatt taacagttga aataaagaaa
    13021 cttagtaaag agaaaaaaac tcttaaagca tcattaataa atgaaaagag tataaaagca
    13081 agacttagag aatttagaaa gatacttgaa agaaatgaat tgttagaaaa atttgataga
    13141 tatgtatttg caagtatagt agataaggtt atagttggag ttattaatga agatggtata
    13201 gttgatcctt ataaaataac ttttgtttat aaaactagct ttgatgataa acaagatgga
    13261 cataaattta taaataagag tccgttagaa acgtattcaa aagataacaa cgataataaa
    13321 aagaagtgtt catatactaa taatgagaca ttgcgagact gtagttaaac ttattagaga
    13381 ataacaaggt ttcagacttt tatattgtgt cttaaaagta gatttgccac ctatttgcca
    13441 ccataattat ttttgggtgg caaatttatt ctacaatgat ttcaaaaata tttaccgtct
    13501 ggtttctcat gttgttagtt acatgtaaat atgtatccat agttgttgaa gttttagcat
    13561 gtccgagtat atgctggata tcttttatat tagtaccatt ttctaataac atagtatcag
    13621 ggtttttaaa ataaagtgtg tattcttcta aagtagtgat aattaaaata tcaaatatca
    13681 ctatacaaag gaaagtgcac gctttttatg tttaacaaaa acgaaatatt aaaaaactta
    13741 ttgaagaata tgatgtcaaa acaactacag atattcaaga tatgctaaaa gatttattta
    13801 caaatacaat tcatcaaata tttacattgt cgaattttag cggtttaaac ataatatatt
    13861 gactgatata ataaaatcat agaatatttt agggggaaat agattatgtt taagaaaaag
    13921 ttaatagctg ttgcaatgag tataactatg attagtgtag ggagcttttc gtatgctcat
    13981 tcaggaagaa ctgattcatc aggaggtcat cgtgataata aaaataaatc tggattagga
    14041 agttatcact atcattgtgg tggttatcca gctcatttac ataataatgg tggttgtcct
    14101 tatactggag gaggaagcag ttctggaaca acaacttcgg ttaataatga agaaaagcaa
    14161 aagaggtctg ttggagaaaa aggatataat caagggtatg aagatgggta taaaggaaat
    14221 tatagttcat caaattatag tggagattat agtgatacat atgaatcaaa gtactcagaa
    14281 ggatatgaaa aaggtaaggc taaattagaa gaggaagaaa aggtggctaa agagactgga
    14341 tataatttag ggcttacagg tgctaaatct aataatacat atgaaaaaga agttttaaaa
    14401 aatgcttatg ataccggata ttcaactgga tacaatgaat ataagactaa aaagatagaa
    14461 gaatataaag ctaaagggat agaggatagc aataaagata aggaaaaaat gacatttgaa
    14521 gaaaacatag attctgaatt tatagatgct tataataatg cttatgacga aatacaagaa
    14581 caattaaaaa atgattacac tactcaagga tttgagagtg ctatcaaagg agaaagattt
    14641 gacacatcaa ctataggtaa tgttaaatat gctaattggt ttaaagaagg atatgataat
    14701 ggtaaggtta agttacctaa agcaaaagaa agtgtatata atcaaggtta caatgaagaa
    14761 gattttagta taccagatga aatgaagtct attgaaacta agttaaaagg tgtatatgat
    14821 gaaggtttag aaaaaagaga agaagagaaa agtagaaatg ctacttatgg ggttggagct
    14881 ggaacagcag ctgtagttgc tggagtagta tataagaaga aaaaacgtaa aaagatatag
    14941 atgctagata taccaatgat gacaagtgta taacaaatat aattttaagt ataaatattt
    15001 aaaatataga agggaaggtt atatctatgg gatttaactt taggaaaagc ataaatctag
    15061 gaggaggact caaattaaat ttaggtaaga agagtgtagg tataagtgct ggtgttaaag
    15121 gtgctagagt gtcagttaat tcaaaaggaa gaaaatcagc tacattgagt atacctggaa
    15181 ctggaatatc atatacaaaa actagtacaa agaaacgtaa acctaaataa atatttgata
    15241 tactaagaaa tagactgtta caaatagtct attttttagt atatagattt aaaaagtgac
    15301 aaaattttga aatataaact aatttcataa aggaaaatat tattttatgt agaattataa
    15361 tatttaagta atattataaa atttatagga ggatttcatt atgaaattaa gcaaaatact
    15421 attggcaata gggatgtctg tttcattatt gattgctagt tgtccaacat caaacgctct
    15481 tagttctatt aattcaataa aaggtacaaa taagtatgaa acagcaggtt taatagcaga
    15541 taaacaaaat tatgatattg caatacttat taattctgaa aatggattat ctgatggatt
    15601 gtcagcaagt ggattgtcag gagctaaaaa tgccccaata ttgttaacta agaaagatac
    15661 aatcccttca gaaacattaa aaagattaga aaaggtaaaa gaagtatatc taattggagg
    15721 aaaaaattcg ataggagcat cagttcagaa aatattaagt gataaaaaaa taagcataaa
    15781 tagaataagt ggaactgata gaatagatac aagtcataaa gttgcaaaag aaataggaag
    15841 agtaagtacg gttaaaaaag taatattaac taatgctttt aaaggcgaac cagatgctat
    15901 gagtgttgct tctatagcag ttagagataa atctccaata gtattaactg atggaaatac
    15961 tacaagtttt gctacagatg gattagaaac ttatgcaatt ggtggaaaag tcagtatgag
    16021 tgataagtta ataaatgaaa ctaaagcaac aagaataggt ggagtagata gatacgatac
    16081 aaataaacaa ataataaata aattttataa tggctcaaaa gaattttaca tagcaagtgg
    16141 gactgattta gtttattctt tagttggttc tacaattgca caaaaaacac ctatagtgtt
    16201 agttggaaat ggaagtaata agagtatatt aaaaaatgca agtaaaataa ctgctattgg
    16261 aaatattgat gatgttgtta taaaacaatg tttaaatata actaataatg ttggggattc
    16321 aaatacaggt attgtaaaag atactaattc aaataatacg aacaaaccaa gtaatgagcc
    16381 tagctctaat aatcctggat taactgataa aagtacagta tatgtaactc ctaatggaaa
    16441 atcatatcat ctaacaaaaa attgttctac actaaaaaga agtaaaacaa taaataaagt
    16501 aacattaaaa caagctaagt cacaaggtaa aatagatcct tgtaatgttt gtgtaaaata
    16561 atgtattagc caaatatata ttaaaaaaga caattaatta agattgtctt ttttattgaa
    16621 gaaatataaa tttatattat gtattattaa aaaaaaatta ttaattataa aaaataataa
    16681 taaagacgat aagtattaaa attttatgtt tccaatattt atgatataat taatatggca
    16741 aggaataaat atttgaaaaa gctatgagtg gtgtttccat ggaagtttct tcactttctt
    16801 atgagaggag gtgaaaacta tggaaaatct aatgatgagt attataactg gtgttatagc
    16861 cagttacatt tacgataaat taaagagcca ctctgagcga ccaactaaga gtggctggga
    16921 acttaatata aagttccaga aaagtaaatc ttcaaaataa tttgttgaag gaaacatcac
    16981 tcaaagttaa gtaaaatatg tttccttgct tttattatat cacaaattaa aaatattaac
    17041 acaaaatttt gtgaatatat acttattatt gttctaaaaa aatatgtaaa taattgattt
    17101 gattgcatgg aattattgat gtaatattta agttaaatat acacaatctc aattaaagaa
    17161 gataagtact ataaattcat atcaaaaaaa tacataaact tttaagttat aattttataa
    17221 aaagtctttt agtatcatta tttcaagagg aattatcata tgaaaaaata acttcattag
    17281 aaaagtggat aaaaaactgg taagaaga
//
LOCUS       DAELTA010000082        17071 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.082, whole genome shotgun sequence.
ACCESSION   DAELTA010000082 DAELTA010000000
VERSION     DAELTA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.082"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(165..689)
                     /locus_tag="KPG13_002247"
     CDS             complement(165..689)
                     /locus_tag="KPG13_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG0985958.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(754..1506)
                     /locus_tag="KPG13_002248"
     CDS             complement(754..1506)
                     /locus_tag="KPG13_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0985959.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1537..2835)
                     /locus_tag="KPG13_002249"
     CDS             complement(1537..2835)
                     /locus_tag="KPG13_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG0985960.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2852..5116)
                     /locus_tag="KPG13_002250"
     CDS             complement(2852..5116)
                     /locus_tag="KPG13_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG0985961.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5371..6189)
                     /locus_tag="KPG13_002251"
     CDS             complement(5371..6189)
                     /locus_tag="KPG13_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG0985962.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6182..6880)
                     /gene="cbiQ"
                     /locus_tag="KPG13_002252"
     CDS             complement(6182..6880)
                     /gene="cbiQ"
                     /locus_tag="KPG13_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG0985963.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6883..7167)
                     /locus_tag="KPG13_002253"
     CDS             complement(6883..7167)
                     /locus_tag="KPG13_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0985964.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7157..7909)
                     /locus_tag="KPG13_002254"
     CDS             complement(7157..7909)
                     /locus_tag="KPG13_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG0985965.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8091..8283)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8415..10187)
                     /locus_tag="KPG13_002255"
     CDS             complement(8415..10187)
                     /locus_tag="KPG13_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG0985966.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10201..11130)
                     /locus_tag="KPG13_002256"
     CDS             complement(10201..11130)
                     /locus_tag="KPG13_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0985967.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11351..11536
                     /locus_tag="KPG13_002257"
     CDS             11351..11536
                     /locus_tag="KPG13_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0985968.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11529..12230
                     /locus_tag="KPG13_002258"
     CDS             11529..12230
                     /locus_tag="KPG13_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0985969.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12232..13155
                     /locus_tag="KPG13_002259"
     CDS             12232..13155
                     /locus_tag="KPG13_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0985970.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13312..14235
                     /locus_tag="KPG13_002260"
     CDS             13312..14235
                     /locus_tag="KPG13_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0985971.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14228..14965
                     /locus_tag="KPG13_002261"
     CDS             14228..14965
                     /locus_tag="KPG13_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985972.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            14976..15749
                     /locus_tag="KPG13_002262"
     CDS             14976..15749
                     /locus_tag="KPG13_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0985973.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16290..16898)
                     /locus_tag="KPG13_002263"
     CDS             complement(16290..16898)
                     /locus_tag="KPG13_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG0985974.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5505 a         2956 c         2156 g         6454 t
ORIGIN      
        1 tttttagtta aaatcataca atatatagat ttccacaata caaataaatt aaaaagtgaa
       61 atgtatttgt ctaaaaacat aataacattt cactttatcc tcatttttaa gtaatatata
      121 tttttaattt ttatttataa ctaaatctag tttttatata aattttagtt tttttctaca
      181 tctgagtaat ctattccaaa agtttctaca gtaacagatt tcataacttg tggagtttgt
      241 ggcttatcag ctgaatctgt tctaactgat gctatttcat caactgtatc cataccttct
      301 atcactctac caaaacctgc atactgacca tctaagtgtg gtgagttttt atgcattatg
      361 aaaaattgag aaccagctga attaggtgcc atagttctag ccattgatat gactcctctt
      421 tcatgtttta aattgttagt aaatccatta ccactaaatt caccttttat tgagtgacct
      481 ggaccaccca ttcctgtacc atttgggcat cctccttgta tcataaatcc tggtattact
      541 ctatgaaata ttattccatt gtaatatcct tcatttacta atgatacaaa gtttttaact
      601 gtatttggag ctatatttgg atataactca attttgattt cttttccatt ttccatttct
      661 atagttacta taggattttt attttccata atttattacc ttccttttca ttagttaaat
      721 tacataatta catttattat attatactct aaattaaagt ctatcaagta tattttttcc
      781 tatttcatat ccttcttgat aaatagtttt acaaaactca tcatcaaagt taagcgtgtc
      841 tttaggagca aattgagtat taggtctaac tactattatt ttatcttcat catatacttt
      901 tttaatatct tctggcaact cataattata ttttgtggat attaaaataa ttttatccac
      961 attatcccca attaatggtt caattaatgc actcctaatt aaacctccat ctaatttgaa
     1021 accatcataa attccttctt gcacatcttt cacaaattgt tctttaaatc caagagttgc
     1081 gtttggattt gatggtaaca acccactgta tgaaatgctg ataatggctt cattacgagg
     1141 catttttgat acgtcaacaa tcttttctga tactacttta ttatcaactt caacataatt
     1201 aacataaaag ttcatttctt ctatattact atcatctagt tcccttaggt tgtctatagc
     1261 aggagaatta tccacagtat tatttactat ttttacattt ccattctgga tatcttttat
     1321 atttgtccac attttctcca agttatcaac attccctgtg tacaaataat atccatttat
     1381 tgctcctatt gatgtccctg aaatcgaata aaatttattt attcctccat catacaaggc
     1441 tttaactact cctgcttgat aagccccttt tgctcctcca ccttctaaac ataatcctaa
     1501 tttcatagtc tcatctccaa attgtttatc ttatatttat atttcaaaaa acttactaaa
     1561 tgttttatat atactatagt ggtcctcaac atttcctaat tctcttatag aatgcattgc
     1621 taaaattgga cttcccacat ccacagatgg tatatctata tgagtagaag atattggacc
     1681 tatagtactt cctcctcttt catctgacct attaacaaat tcttgatact caacattagc
     1741 ttctctacat atatttttat aaatagctat agaatatcca tcactagtat atgcctgact
     1801 agcatttatc tttataaccg gaccttttcc cattattggt ttattagttg ggtcatgctt
     1861 ttctggaatg tttggatgaa tagcatgagc taaatctgat gatatcataa aagatgaata
     1921 aatagactca aagaatccct ctctatcctt acccattgat atgcaaattc tctccaatat
     1981 atttaacaac atgtttgaat ctgcaccttg tttagtagag ctaccaactt cttcattgtc
     2041 aaatacagcc attacattta caccatgttg acctttagta ttaacaagag cattcaaact
     2101 agcatgtacc attgcaagat tatcttgtct cccaatagat ataaattcat cattagcccc
     2161 tattaaactt cctttttcaa attcatataa aaataaatca aaatctataa tatcgtccat
     2221 atttacatct aagttagatg ctatttgctt tataagaaaa ttatcttttt ctaatgtatc
     2281 atttaacatc ccaactaaag gtaacatatc tttttgctta ttatatgagt gaccatcatt
     2341 tatacttcta tttaaatgta ttgctagatt tggtattata caaattggct ttttaaagtc
     2401 tataagcctt tcatttggtt ttaatatact atcacctttt aaaactactc taccagctat
     2461 cccaagaggt ctatccaacc atgtacttaa tattgctcct ccatagcatt ctgtatttaa
     2521 cttcaaataa gatttttcag cttctatttc tgcatttggt ttaatactaa atgtaggaga
     2581 atctgaatga gagcctatta ttctaaatcc atctctttct ataccttctg aatttactac
     2641 aaaagctact atagctgata aattcttagt aacataatac tttccaccaa ctcttagatt
     2701 ccatctttga ttcaaaacta attcttcaaa tccattttta tctaagattt ccttagtact
     2761 ctcgactgca tgaaaagacg acggactatc atatatgaag tctattaagt ttttagcaaa
     2821 gtttatacta tccattcctc atttacctcc attataaaat atttagtgtt gtatattctt
     2881 tatttatttt tttaattccc tgttggtcaa aagctatagt aacatctgta ccaattatag
     2941 aaaccactgt cccaacccca aatttaggat gatgtacctt agaaccaagt tttatatcat
     3001 ctggattagt ttctttatta gaatctttta ttgtagcatt aactttatct gcaactttag
     3061 atttgcttat agtactcata tactttttag tatatttatc taacatatta taacttgctt
     3121 tagaatagct tagttctttt tttactttgt acaatctttc tatacattct tctggcagtt
     3181 cttccataaa tcttgaagct attgcaacat ttgttttacc atataatgtt ctcttctctg
     3241 tcaatgacat atatagctct tctttagctc tagttatacc aacataacaa agtcttctct
     3301 cttcttctat ttgtgaatca ctcatagatt ttatagctct tgatatagga aataaccctt
     3361 cttccatacc tgttaaaaac acaacaggga actctaaacc tttggatgta tgaattgtca
     3421 taagcgaaac cctatcattg tcttcttcat catttgattc agatgtaagt gctatacttg
     3481 ttaggaatgt ctctaagtct ttattttcat aagtatcatc attactactt tcaaactcca
     3541 aagctataga aataaattct ctcaaattat caattctgtc ttgagcttct tcacctttgc
     3601 ctgttaaatc tttttcattt ttatttctta tctctacaag ctcgtccata tatcctgtag
     3661 tatccaagac tttttctata agtttgctaa caggataaac ttcttttata gttctaagag
     3721 tccctataat atcaacaaat ccacttatac ttgcccttgc cttagtagat atatctgaat
     3781 ttgtttctat atcaattaat actgagtata tactttcctg ttttaagtta gctctatctt
     3841 caattttttc tatagttcta agccctatac ctcttctagg aacatttata atccttttta
     3901 tagatatatc atcttgtgga ttttgaataa ctcttaaata cgccaacaaa tcctttattt
     3961 cttttctctc atagaatttt gtaccaccat atatattgta tggaatttga cttctattta
     4021 aagcgtcctc tacaggtctt gcctgtgcat ttgctctata aagtactgca aaatccttat
     4081 aaggtctatt ttcttttcta gctatttgtg caattgaatc agcaatgaaa tcagcctctt
     4141 ctatttcact accagttaat tgtatcttta taagttctcc ttcttttttt tcactccaaa
     4201 gcttttttct ttttctttct atattattag atataacttt attagctgca tctaatataa
     4261 cttgtgtaga cctataattt tgttccaact ttacaacatg tacattgtca taatctttct
     4321 caaattcaag tatgtttctt atatctgcac ctctccatcc ataaatactt tggtcatcat
     4381 ctccaacaac acaaatattt tgatgttctc ttgctaataa ttttatcaat tcatattgag
     4441 ctttacttgt atcttggtac tcatctacca ttatgtatct aaatctactt ctgtaatatg
     4501 ctaacacttc atcattagcc ttgaaaagtt ctacagtttt aagtattaaa tcatcaaagt
     4561 caagtgcact atttcttttt aaacggtctt gatatagcgc ataaatatct gcaatcttaa
     4621 ccattctatt atctgccata ttgatatctt taaactgctt tggagtataa agtttgtctt
     4681 ttgctcctga aattgcactt ataactgctt ttggttcaaa cactttatca cttaaattta
     4741 attcttttag acagtcttta accaatgtaa cttggtctgc tgaatcataa attacaaaac
     4801 ttctattgta accaatctta tttatatctt ttcttagtat acgaacacag caagaatgga
     4861 atgtacttat ccacatatcc ttagtttcag gacctatatt ttgttcaact ctctctctca
     4921 tttcatttgc agctttgttt gtaaatgtta tagctaatat atttggtgct tgtacacctt
     4981 tatcttctat aagatatgct attcttgttg ttaatacctt agttttacct gaaccagcac
     5041 cagccagtat aagtactgga ccttcagtct tttctactgc ttctctttga gcaggattta
     5101 atgtatctaa attcatattt ctcctcctgt taacagccat aaatatattt taatctaaaa
     5161 tatgaattac ttcaaattat aattatgtat tatattaata gattatttcc aatatattac
     5221 aattaaataa catttttcca tataatttat ttcattgttt aataaaaaaa ataaccttat
     5281 gatttgggga atcataaggc acctttaagg ggtctaggtt aagggttatt atatataaat
     5341 ctattttttt aaaataggtt tagggcttaa ttatttattt gtattaagtt ctctttcctt
     5401 ccaatactta tacaaatctt cttctttctt aaataagggt aaattcatat ttctatgtac
     5461 ttttactaac catggggatt ctaggccagc ttgtataata ttattttcat ttataaacac
     5521 attttctgct tttccttcat caatcaaaat ccccttatcc aatacatata tgtaatcaca
     5581 tatttcatac attaaattca tatcatgact ggatattact atttttatat tatttttatt
     5641 taatcccttt attatatcta caatagacct agtacttact gggtctaaac cagctgtagg
     5701 ttcatcaagt aatacaattt cattttccat tgctataact gatgctattg ccactctttt
     5761 tttctgtcca tagctaagaa aatgtactgg cctgtctata aaatctattc cattaacagc
     5821 tactagagct ttgtttattc tctctttaat tgttttttca tccataccaa tgtttctcat
     5881 tgcaaaggct atatcatcat atacttttga ataaaaaatt tgtttttctg ggtcttggaa
     5941 gacaatacct acattttttc taaggttgta taaacttctc ttatcatatt ttaacttttc
     6001 ttctttaaaa agaatttctc cagatgtagg ctttaatata cccattaaat tcataaataa
     6061 tgttgatttt cctgaaccat ttgaacctat tatacctata acatttccct ttgaaaaatc
     6121 catattaatg tttaatagtg cattggtatt tttttcatat tgatacgtta aattatttat
     6181 tttaaacatc attatcccct actatgtgaa atttaccatc atacaatttt atgtctaatg
     6241 atatacacat atcatcatac cttgtcataa gtcttttgta cagcatactt ccaagaatac
     6301 ctaaggattt atatgaagtt tttaaattta tatatccaaa tcttagctct tgtgacttgt
     6361 atatttctga aaattcttct aaaaatatga atataaacct atatattaac attgataact
     6421 cgacaaaagt gtcaggtaaa tgaagttttt taaaaaagaa tattaattga ttaaaaggtg
     6481 ttgtaagtat acaaaaatac acacaagtca aacaagacat cgctctaaat aacacatgaa
     6541 tagacatgtt aatagacgcc tttgatattc ctacattaaa actaaaaaat tcaaaactat
     6601 ataataaatc tgctttatta aaagatatat ttacaagagt tattcctata ctaagaaata
     6661 aaaagtacat tggtattcta agtaatttta aataattttt taaatctatt ccagccacac
     6721 aaacaaccaa aatactcatt aaaatcataa ttccacttaa aataaatata ttttgtatta
     6781 ccatagaagc aatcagaaaa attactccta tggctacttt tttgtttgga tttactttac
     6841 tcaatctatt tgtatatgca tatttatcaa ttataagcat gattaacttc tctttccttt
     6901 ttgaagacct aatatgtatc ctattacacc agcccctaaa gctgcttgtg cagagaataa
     6961 taaactttct atttctccac ttggtggctc atatggactg ctaaaccatg gtttgtaatt
     7021 tggatttatc ttagtaattt cactttcagc ttgaccatct gctcctccat attcagcacc
     7081 tgagttgaca agtaatggga atataataag agctactact aataaaagta ataatatgtt
     7141 agtttttgtt tttgttttag cactcattag ctttaactcc tttatcatta ttttctagta
     7201 tgcttttttc tccgttttct gtaattaaat tatatacaat tacagtaagt ataccttctg
     7261 ctattgctat tggtatttga gtcataaaga aaattcctaa aaatttaatt gctgaagcca
     7321 ttaccccacc tgatgggtct gggaaagcca gtgctaattg tattgaagtt attgtatatg
     7381 tggctaagtc tcctattgct gctgcaagaa atactggaat agcattattt ccatcttttt
     7441 tctttaaagc cttaaatatt aggaatgata cgattggccc tattatcgcc attgaaaaag
     7501 cattagctcc taaagtagtt attcctccat gtgctaataa caatgcttgg aatattaaaa
     7561 ctatagttcc aagaacaaac attacgcttg gtccaaacat tattgctcca agacctacac
     7621 cagtaggatg tgaacaactt cctgttactg aaggtatttt tagtgctgat aatacaaaca
     7681 caaatgctcc acataaagct aataatactt tctttttagg gtcttgcttt actattttac
     7741 ctattgattt taatccgaat aagaaaaatg gtataaatat aactccccat gctatagacc
     7801 attttactgg taagtaacct tccattatat gcattgcatt tgatgtaact ggagttaaaa
     7861 tgattaacat aagtgcagct ataaccccta atttaatatt ctgtttcatc ttagtatccc
     7921 tctctccctt tctaataatt aaaacataat aatacatgtc tgatttattt aataaaataa
     7981 aaaagcctaa ctcgatggtt aggcttctaa ttttccacaa tctaaataca aacacataaa
     8041 acaaacatat ctgtataatt attataagaa actttctcca tccccgaaga aattacttta
     8101 atattttagg caggtctcct gacttagctt catcctactc cttttccttc ccatgagata
     8161 taaaaaatat catcatagtg gctaacaaaa gtttcgtctg catcacagta gcgggggctg
     8221 taaaggaatt taaccttttt ccctattaat cttgcttaaa gaacctataa tattatatat
     8281 ttaattttta aattattctt aaatcatttt ctatataaat cattgtatac cttttctgtg
     8341 aaaatttcta cattttttat aaattttatt catttttata tttttatgta tataaattct
     8401 tttttgtaag tttattaatt tctaattatc ttctttaaat acattgaaac tacatcagaa
     8461 aatctagtta tatctcttga atatataaaa tcatttccat atattaatct ttcagcttca
     8521 agttctgatt cttttcctgt aaatactcct aatactaata tattttgctt tctagctttt
     8581 ctcacttcat tggctgtatc tctcaaacca actatacccc tataagcctt ttctcctctt
     8641 aatgttctct cactactttt tcctatcttt acatcatttg gtcttccatc gctgagaact
     8701 atcaggattt tattttcttc atctctattt attagaccac tacatataga ttttattgcc
     8761 aaaccatctc tattattacc agcacaaaaa tattcaaata tattttcact attttttata
     8821 ctatcatcat aatctttaaa tctttttaat attgtatagt ctaaaaaact agaaaatccc
     8881 atcactctat ttggtatgcc aacttctgtt agagcatttg caattatgta agcttgaatt
     8941 gccacatttc cctgatttct actttgagag ccacttgcat ccaataatat atcaataacg
     9001 tatttccctt tttcattttc tatatcttta taaaatattt tattattatt acttctacca
     9061 actctccaag ctctattggc taatatagta ccacaatcag aatatactct acttttagca
     9121 ttttcttcta ttaaaattct tgacagactt tcttttaatc tctttatttg ctgtctatac
     9181 attctagcat gttctctaaa ttttcctaaa ttattttctt tttgtcttgt tacatatttt
     9241 agttgaaata cattattaca ctcacttcta agaactccat ctgtaaagtg tattctacaa
     9301 ccttcatgaa catttctaca attttgtgtc tcaatttttt taatttcacc tttactcaaa
     9361 taagtttttc cataataatg ttctattttt gaatatatct tatttgcagt gtcttcatca
     9421 acatatataa ctcggtctgc tgaattgcta catttagaat catcaaaatc ccctacacct
     9481 tctacaagca tggatgaaga tattgaatct atatcacttt caataatatt ttcttcttca
     9541 tcactataaa gttcttcata catgaaatca gcaaatgtat caaaatttac ttctacactt
     9601 tctacattat tttcatcaat aagactattt tcttctccat ttaatatgta ttcaaaataa
     9661 gttaaatata taacatctaa tgcttttaaa atctccataa tatcttgatt ggtatctata
     9721 tttcttatat cattaactat tcttctagtc atactatcta atgctgggtg cttgtccata
     9781 ctataaagta caaatgtgta tttaatttta tccataatat tatcattgtg tattttggta
     9841 aaacgtgaaa caatatcttt ataagcattc cttcttatgt cttctatccc tggcctgtct
     9901 ttaatgacct tttcttcaac tattgtattt aatataactt gaattagacc tagtataaca
     9961 tctttgttgt atccttttct tattctattt gtaagatatt ttttaactat atcccaatct
    10021 acatactttc ttctagcacc tgcaattatt gcgaaatata gacctatttc ttttgaagag
    10081 taatcttttt ctgatatatc tatactttca ttgtaatcac cagctattgt ccatgatagg
    10141 ttattagctc tattttcaaa ttcataatca tcatatatcc atttcataat ctcacctata
    10201 ttaaatatga cttattggaa acacatctat actctcccat ttattaggta ttctagtttt
    10261 aattacatct ccaaccattt ctttctcata tacatcaaaa gtctttcctg taagtcccat
    10321 attaattgca agagttggtt ttaatcctct tcttatagtc ttaagagatg ccataagacc
    10381 acgtaaatca atagctttac tagatatctc tccattttga gacttcaatt gtaaatctaa
    10441 aaatattcct gcaaagtgta ttaatttttc ttcatcagca tctgaaaatt cattttttaa
    10501 aatcatcatt aacttatctt cttctatagg aggaatatct attaccataa atctagatac
    10561 cagtgcttca ttaagttctt ttgttcctgc atattcataa ttcatagtcc caataaatct
    10621 tgttgctgga tgtagattga ctctctcata cccaggtaca tcaattattc ttctatagtc
    10681 taaagctgaa tgtaccacaa ctatagcatc attttttgcc atattaattt catcaaacac
    10741 cccaaaacct ccattaatag cacactcata aactgagcct cttctaagtt taacttcatt
    10801 gtctataaat gtatctgtac ctataagtgt tgaactgtct gtatttatat gaaatgatgt
    10861 attccactga ggtctattaa atatttcccc tagattatca gcaagtaagt tttttcctgt
    10921 tgcctttggt cccgataata atatattttc ttcttctaaa atagctgcta tacacataga
    10981 caagatatct ttaccataaa aataagtttt agactttgtt actctgtatt ctaaatcttt
    11041 ttctaaacca taataatttc taaaatacag tacatcttca ataagttttt catctacccc
    11101 ttgcaccctt agattatcta ttatatccat atttttaccc cttttatata aatagtattt
    11161 gtttcatttc ttatcaccat tttagcataa aaggatataa gctaaaattg attttttaat
    11221 ctccccattt taagtaaaac catgcaccat tttatcacta caaaattaag agtactactt
    11281 actgatattt cttgattttg ttcgtctata cgtatataat atatgctgat aaattatgag
    11341 gaaaggatga ttgaaatatg gatgcccaga agtagcaaaa aaatggggta tttctgaaag
    11401 aagagtacaa aaactatgtg aagaaagtcg cataccagga gtttcaaaac tcgggtatat
    11461 gtggctgata ccaaagaacg cagaaaagcc tgttgatggt agaacaaaaa gaagaaagga
    11521 agtacaatat gaatagcgtt ttgattatag atgatgacaa agaactttgt gctcttatga
    11581 aaaaatgtgt tgagcaagaa aatttatctg ctattactgc acattgtggt gcagaaggtc
    11641 tacatttggt cgataaaaat agacacacac tctctcttgt aattttggac ataatgatgc
    11701 cagatataga cggttttcaa gttctgaaaa aaattagaga aacaagtaat attccagtaa
    11761 ttatgcttac tgctaaaaat gacgaggatg ataaagtttc cggactacgt ttaggtgctg
    11821 acgattacct aacaaaaccg ttcagcataa acgaacttat ggctcgtgtc aattctctta
    11881 ttcgtcggta tactatgtta aaccctgctt ttataacaga cacagattgc ataataataa
    11941 atggcatgat tattgacaag acaaaccgaa ttgtttctgt caataatatt cctgttcaac
    12001 ttacaagcaa agaatttgat ttacttgctt ttctggcttc taataaggga cgggtgttta
    12061 ccaaaaaaca gatttataca caagtatggg aagaagaata tgcttttgac gatagtaaca
    12121 tcatgtcttt catcagtaaa ttgagaaaaa agattgaacc agaccccaac catccatttt
    12181 atattttgac agttcgtggt gtcggttatc gttttaataa ggaggcttaa catgaataca
    12241 agtgtctatc tgcttttgat attggttctt agcctgatta ttatagcgta ccttgtttct
    12301 gtcctgctac gtgtccgaat acaacttaca cttataaaag atgctttaga ggatataaaa
    12361 aatggaaacc ttaatcgacg tattttaaca gatgaaaacg acattacaaa acaaatttgc
    12421 tatgatatta acgaaatagc aatcaatagc caatttcaac ttattcagca aaaacaatca
    12481 gaacaagcct acaaacagct aatgacaagt ctctcccatg atgtaaaaac tccacttgct
    12541 tctttggtcg gttatttgga agcagtagaa agtaaaattg ttgtaggaga ggaaaaagac
    12601 gagtatatcc atgttgcgtc taataaggct cattacttaa agaattttgt agaaaatcta
    12661 tttgaatggg taaaattgga ttctaaggaa caagtttttc attttgatat ttttgacttg
    12721 aatgaattat cccgtaatat tatatctgat tggatacctg ttttagaaag cagtcatttt
    12781 gaatatgaat ttgatatacc agaaatagag tacttcttac gtatagacgc aaatgcatat
    12841 acccgtatca ttaataacct attacaaaat attattaccc atagtagtgg caacaaaatg
    12901 acattgagaa tttttgaaaa caaagagcaa gctcaaatta taataacaga taatgggaaa
    12961 ggtatatctt cagacaatct cccacatatt tttgaaaggt tgtaccaatg cgaccattca
    13021 cgagcttcaa agggaaatgg tttaggcttg gcgattgcaa aggaacttat taacgctcat
    13081 aagggaaata ttaccgccaa cagcactccc ggtatgggaa cagaatttac aatattgctt
    13141 cctaaagccc tgtaaaagta cgaggctttc ttactctaaa acaagataaa agcaagatta
    13201 tggcaaggtt ttggcaaggt tcatgtttta taattctaat tgtagttgat tttgacgttg
    13261 tatcaactat cttttgtaga aacaaattta ttaaaagtga ggaaattata tatgaatgat
    13321 tttgtgattg aaacaaagca acttacaaaa atttatggga atcaaacagt agttgacaaa
    13381 gtcaatctcc atgcaaaaaa aggccgaata tatggtctgt taggacgcaa cggagctggg
    13441 aaaaccacaa ttatgaaaat gattttagga ttgacttcaa tcacgtatgg cgaggtagat
    13501 gtatttggag agaatattaa gggacgtgaa aaacaagtat atccccgtat cggtgcaatt
    13561 atcgaaacgc caggatttta tcctaatctg acaggaacgg agaacttgga gatttttgca
    13621 aaactgcgtg gtacagccgc ccctaattct gtaaaaagag cattggaaat tgtaggctta
    13681 ccttataaaa ataaaaagct gtttagtgaa tattcacttg gcatgaaaca gcgacttggt
    13741 attgccaacg ctatcctgca tgacccagaa ctgttagttt tagatgagcc aaccaatggt
    13801 cttgacccta ttggtattgc ggagatgaga aagtttatta gggacttgag cgttgagcat
    13861 ggaaagacaa ttcttatttc cagtcatatt ctttcagaaa tctcattgtt ggctgatgat
    13921 attggaatta tcgaccacgg tgttcttttg gaagaaagta gtatgagtga actagaaaag
    13981 aaaaacagta aatatatcct gctacaagtt tcagatattc cgaagacatc acttatttta
    14041 gagcgtcaat ttaacacgaa ggattattcc gttcaagatg actacacgtt acacctttat
    14101 aacactaatc tggatatggg tgaaatcaat aaagcacttg taatgcagaa tgtaactgtt
    14161 attagttctc aactttgtaa tgatacttta gaggactact ttaaaaaaat cacaggagga
    14221 gagggcattg cttaaactta ttcaaacaga atttcttaaa ctgcgtcgta ggaaacttat
    14281 ttggctgatg ttgttgtctg ctctcattat gccgtttttc gcactactat acttcaatta
    14341 ttttggtaaa acaggtgttg agccaataaa gttttataaa tggtctgcat ttggttatac
    14401 actttggatt attttgcctg ttgttttggg tatattttgc accatgctta tacacgaaga
    14461 aaaccagtat gatatgctca aacagctttg gattgtacct attagtaaaa tgggatactt
    14521 ttttagtaaa tttttcgttg ttttgattta ctctatttgt tttatgctga ttacagccgt
    14581 tgcttctgtg ttatttagtg tactttctgg atatgtcgta tttagctggg caagcatttt
    14641 atatttgttg aaaaaatgtt tagaaattgg tgtaatcact gcttttgtaa tgttgccgat
    14701 tttggcaatc gctacatctc aaaaaggata cattctccca atttgtatca cattggtata
    14761 tgccttttcc ggttttgtta taatgacaat aaatatgtat ttacacccat tatccagtat
    14821 gggagttatt atcatgagaa ataaagacat tccaggtctt atattttcac aggaaattaa
    14881 tgtcccatta gcatttcttt gtatttgtat ttggagtatt gtatcagtat tgtttgcaaa
    14941 cactgcacta ggaagaagaa agtgaggtgt agataatgtg gaaattactt tgggctgaat
    15001 gtcaaaaaat tcgtcgttca aaaattgttt ggattacagt ttttgcatcc attatgatag
    15061 cagtcatagt ttttgcagga gggcaggatg tatataatga cccagattta cattatggct
    15121 taaagagcgt ttatgacggt tcacggtata tagacaatgc aggttggtat atggacgcag
    15181 tacaaccttg gtcaactttt tttgttcttc ctgctgtcat tgccctatta ggaagctata
    15241 ttatttgtcg tgaagaagag gaggacacta ttaaatccct gcgtcttatt cccataaatg
    15301 aaacaaaatt aaccatagca aaaatgatta ttacttttat attcagtata cttctttatt
    15361 tgttactctt tactataaca tttttgactg aatccgtttt acatttttcc gatttatcga
    15421 caaaactggt tttaagttgc atgaaagagt attttcttga tggcataggt gtattctttg
    15481 caatttctcc cattattgcc ttggtatcgc gcatgaaaaa aggatattgg cttgcattag
    15541 tattcacaga agtttattct attgctggac tatttgcggg tatgtcaaat gtcttacaaa
    15601 ccttttttcc tattgcggca atatttaata tttctggata ctatataact actggagaaa
    15661 aaattatggg gagtatcata agtttattac tttgtgcctg cctttctgca tttatactta
    15721 aagggctaaa acacaatgaa aaaaattgaa ataggtatat caaaatgaaa aagataaaaa
    15781 aactgattat tctaattgta ttcttgtttc tttttggcat gttacactgt tatactgaat
    15841 ggaaattcaa caataaccca cctaaagggg taatagaaag aataaatgaa tggactgata
    15901 agattggact ggattagtaa cttatgttaa ttatagtgaa tgtaatatta gcaagttatt
    15961 gtttcgtatt ttaatactat aactttaaat atttggtttt aatcttccaa acgaatataa
    16021 aaaaactgtc acagaggcaa catatttttg aatatttata aagtggtagt tttgattttc
    16081 aaagctgccg ccttttcttc tatataagca aacttatcct caacaaagtg taaaacatgg
    16141 tacattttaa gaaaaaagta ccacagacac aagcaataaa agaaacgccg agaacctaca
    16201 aaaacgtagt gcttatgcag atttacgacg ccttctattt tctcaaccgt cttaatgacc
    16261 ttgtacaaaa gaaatttttc taatattttt tataatctta tcttgtgatg ttttttatgt
    16321 ataaatgact taataacaaa tccaataaaa attatggata ttggtataat tagaacgagg
    16381 aatttataat cattataatg tgacattatt cttacccaac tctcagaaaa aatatatcct
    16441 atactgatta atgaagtatt ccatatggtt attcccacga tagatgatat tacaaaagtt
    16501 ataatatttt gacctgctat acctgctata aaagatatgt atgttctaca taaagggatt
    16561 aatctacata cacaaacaga aatagaagag tatttgttaa aataatcttg agatgcaaat
    16621 attcctttct ttgcctttgg acatatctct attattttat ttatgataga tttacctccc
    16681 caatttccca ctgtataaca aaatatcgaa ccaagtatac ctgcaattat acttaaaatt
    16741 aaaattgtca gaaatccaaa atgatttctt gcagcaattg ctccacataa aggtaaaaca
    16801 acttcgctag gtaatggaaa acaagcatac tcaatcataa ttaatacaaa tacagagatt
    16861 aatccatatt gtaaaataaa actattgatt acagacactt acttatctat ctcctttctt
    16921 atttaaaggt tttaagggta catgttctaa ggattctcac cctatattta aagaatcaat
    16981 ctctttaaac tattatatgt atttacacaa agcatttata acatacttta aaaatagtac
    17041 cttaaaataa gaaacttaat aaataaaaaa a
//
LOCUS       DAELTA010000083        17037 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.083, whole genome shotgun sequence.
ACCESSION   DAELTA010000083 DAELTA010000000
VERSION     DAELTA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.083"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(239..994)
                     /locus_tag="KPG13_002264"
     CDS             complement(239..994)
                     /locus_tag="KPG13_002264"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0985975.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1434..2423
                     /locus_tag="KPG13_002265"
     CDS             1434..2423
                     /locus_tag="KPG13_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG0985976.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2875..3591)
                     /gene="dapD"
                     /locus_tag="KPG13_002266"
     CDS             complement(2875..3591)
                     /gene="dapD"
                     /locus_tag="KPG13_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG0985977.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3815..4564)
                     /locus_tag="KPG13_002267"
     CDS             complement(3815..4564)
                     /locus_tag="KPG13_002267"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG0985978.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4631..5518)
                     /locus_tag="KPG13_002268"
     CDS             complement(4631..5518)
                     /locus_tag="KPG13_002268"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0985979.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5635..6636)
                     /locus_tag="KPG13_002269"
     CDS             complement(5635..6636)
                     /locus_tag="KPG13_002269"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG0985980.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(7061..7942)
                     /locus_tag="KPG13_002270"
     CDS             complement(7061..7942)
                     /locus_tag="KPG13_002270"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG0985981.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8265..9458
                     /locus_tag="KPG13_002271"
     CDS             8265..9458
                     /locus_tag="KPG13_002271"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG0985982.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9664..10782
                     /locus_tag="KPG13_002272"
     CDS             9664..10782
                     /locus_tag="KPG13_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG0985983.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10913..11158
                     /locus_tag="KPG13_002273"
     CDS             10913..11158
                     /locus_tag="KPG13_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG0985984.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            11145..11891
                     /locus_tag="KPG13_002274"
     CDS             11145..11891
                     /locus_tag="KPG13_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0985985.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            12176..13051
                     /gene="hslO"
                     /locus_tag="KPG13_002275"
     CDS             12176..13051
                     /gene="hslO"
                     /locus_tag="KPG13_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG0985986.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13442..14242
                     /locus_tag="KPG13_002276"
     CDS             13442..14242
                     /locus_tag="KPG13_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0985987.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14770..17010
                     /locus_tag="KPG13_002277"
     CDS             14770..17010
                     /locus_tag="KPG13_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG0985988.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
BASE COUNT         6323 a         2171 c         2785 g         5758 t
ORIGIN      
        1 attccatata ccaataaata ggataatatt taataggttt atatgatgaa gagattataa
       61 aataataaac aatataattc catataccaa taaataagat aatctttaag aaaaatgatt
      121 atatttaaaa ggtaaacata aaatttataa acttttagaa tagaaatctc aatcaattag
      181 atattcaaat tgatgaatat gaatacctaa atagattgag atttattatg tacaaaactt
      241 atcctaatat atctgacatt ttataaagcc cagattgctt accaattaaa aatttagctg
      301 caactataga accgtttgca aatacttcat cagattgaga atgatggctt aatgtaagaa
      361 cttcattatc acctgcaaat acaacatcat ggtcgctgat tatattgcca cctcttatag
      421 agtgtattcc aatttcattt ggttttcttt tacaatctct accatatcta ccatgtgtat
      481 tttcactttc aggcaagact tctctaattg cattagctat catagtagca gttccactag
      541 gggcgtcttc ttttctattg tgatgttttt ctatgatttc aatatcaaaa ccttctaaca
      601 tctttgaagc atccttaact aattttatta atatattcac tccaagagat gtatttgatg
      661 ataataatat aggaatgttt tttgatgctt cttctatttt atttaagtca ttttcatcat
      721 atccagtagt agctaatact acaggtgtat tattttttag agcatatgac aatattggct
      781 ctaaagtctc atgatgtgaa aagtctatta ttacatcagt tttttcagtt acatcagtca
      841 ttgttttgta aagctttgta tcactatgat gacctttttc atcagcagac ctagctacac
      901 cacaaaccaa ctctaattca ttatcttttt ttacacattc tgctaaaact cttcccattg
      961 ttccattata gccactaatt ataactttca acataaattc ctcctatgaa attaataaaa
     1021 atttgttata tttctataag tttattttat cactaatata atttatataa aataatatta
     1081 gtgataataa gcttacaatt tatcttacat taaaaaattt aagtggattt ttcaaaatat
     1141 agataaattt ctttttcatt gtataaatga taaaaatata tttgacttca taccaaaaag
     1201 agtgccccat gaactaaaaa gttaattttg aaataccata ttcatatatc atatgattta
     1261 tttggaaatc aatattaata cattaaaata actaaataca tgtatgcata aagatgtata
     1321 aaaaatatat aaacatatag tattaatttt aaattcaaca atttaattgt taaaagtgtt
     1381 attattaaat tagaaatatc ttaacttaat atactgagat gggagtgata gatatgttaa
     1441 aagaagaaaa aacttatcaa gagaaagacg caaaagtatt caatggagtg gcagctttga
     1501 taatgatttt attattttta gtagtttcaa taggtatcat ggtttttggg atagtaaaac
     1561 ttgatagtgg atttactgga ataggttcac ttatgttaat tttgggactt gtatttatag
     1621 tagtagattt tatcctattt tttgggctaa aaatgattaa tcctaaagaa gctatagttc
     1681 tagttttgtt tggtaactat tatggaacta taaaaaaaga agggtactat tgggtgaatc
     1741 cattttgttc agcaataaac ccagcagtat caagaattaa tgtatcgaaa tcttcatctg
     1801 atgaaaaggt agatatatca actaattcaa ggggcaaaaa agtatcttta aaaacaatga
     1861 ctcttaataa tgaaaagcag aaagtcaatg atttattggg aaatccaatt ataataggag
     1921 tggttgtaat ttggaaggtt atagatgcaa caaaggcagt atttaatgta gataattata
     1981 atacattctt atctattcag tgtgattcta ctattcgcaa tgtttcaaga ctttatccat
     2041 atgatgtatc tgaagatgga gatgaaaaat ctcttcgtgg tagtagtcag gaaatagcag
     2101 atagattaaa agatgaatta caatcaaggg ttgatattgc aggaattgaa gtgtgtgaag
     2161 tgagaattac tcatctttct tatgcaccag agattgcagc agcaatgtta caacgtcaac
     2221 aagcagaggc tataatagca gcacgtaaaa agattgttga gggggctgta agtatggtag
     2281 agatggcatt gactaattta agtgaaaata atgttgtgac tttggacgaa gaacgtaaag
     2341 cagctatggt aagtaatctg ttagttgtgc tttgtggcaa taaagatgca caaccagtgg
     2401 taaacagcag ttctatttat taaattataa aaagtttaat agtttatttt attgatatat
     2461 agaaaattta gtagttcatt ttattaaatt atagaaagat taatggtttg ctttattgaa
     2521 ttataaaaag tttaataatt tattctattg atatatagaa aatttagtag ttcattttat
     2581 taaattatat aaagattaat ggttttcact ttattaaatt ataaaaagtt taatagttta
     2641 ttacattgat atattgaaaa attgaaggaa ataaaaatac ttgtcagcaa gaaataaaaa
     2701 tgatataaga atgaataaga gaattgtaaa aaaataaaat gattataaat aaaagataaa
     2761 aatattgtaa aaaagagggt gcctctggtg aattaaattc acacagagac acctttttaa
     2821 ttaatttttt aaattagtat attattgttt aattaatagt atagtactat ttaattaatc
     2881 taaatttcta aggtcttcca taagcttagt tttgtcagcc gttttttcat ctttcatttt
     2941 tataacttta gcaggagaac cagcaacaac ggcaccagct tctacatcag tagttacaac
     3001 agcaccagct gcaacaacag cacctttacc gattctaaca ccttcaagaa taactgcatt
     3061 agctcctatt aaaacatcat cttcaactat aacaggagta gcagaaggtg gctctaaaac
     3121 accagcaaca acagcaccag cacctaagtg aacattttta ccaagagttc ctctagctcc
     3181 aataacagca ttcatatcta ccatagaacc ttcaccaata acagctccta tatttataac
     3241 agctcccatc attacaacag catttttttc tatagtaacc atgtctctta ttattgcacc
     3301 tggctcaatt cttgcatgtt cacttaaagt attttttaga ggaatagcag aatttcttct
     3361 atcatattct acatgagtat ctgttatatc atttccatgt ttatctaata cttccattat
     3421 atcctcatag tcacctacaa atatataaga accattactt ccgaatacct taaatttatc
     3481 agttgattgg aagtgtaaag agttagtatt tacatatacc ttaacaggtg ttttcttttt
     3541 aacttctttt atataccttg ctatttcata agcatcgttt aaatttacca tttgttaatt
     3601 ctccttgtat ttcattatat ttcttttatt atatacttat ttgtcaattt tagtatatac
     3661 ttacttctta ttatttatat tttattttag ttagttttta ttgtatagac atagtattaa
     3721 ataaatttat tatatatgtt tgatttgtat tgcgtattat tatatctata ttgcatatgt
     3781 ttaatttata ttgtgtatta ttatatttat gctattatcc caacatgctg tccatattat
     3841 aaaatcctgg tttttgctta actaagtatt tggcagcagc tatagaacca cttgcaaaga
     3901 taccacgaga ttgagcttga tggcttattg taagaacctc attgtctcca gcaaatagag
     3961 catcatgttc gccaacaata tttccacctc ttatagcatg aataccaact tcatttggtt
     4021 gtctttttgc actacgacca tatctaccat aattattttc aacgtttggc aaaacctctt
     4081 ttatagcgtt agcaatcata acagctgtgc cacttggggc atctactttt ctgttgtgat
     4141 gtttttctat tatttctata tcaaatcctt ctagtaattt agctgcatct ttaaccaatt
     4201 ttactaatac atttacacca agagacatat tatatgattg gaatacagga ataactttag
     4261 ccgcttcacg tattttgttc atttcgtcat cattatatcc agtagtagca attactaaag
     4321 gtgttttagt ctttaaaacg taagataaga tattatctaa attagagtga tgagaaaaat
     4381 ctataactac atcagcttcc tcaactatag tactcatatc ttcataaatt ttaaggtcgc
     4441 cttcatagct atccagacct ctagctgcaa cacaaaccaa ttctaattca ttatcttctc
     4501 ttatagtatc ggctaaaatc ttacccattt ttccatcata accagttgct ataactttta
     4561 acataatttc ctcctatgat taagggctaa cttttgacca aacttacttt ttaccatgca
     4621 ttaatcttta ctatgcatta acttttagcc caaagttaac taattctttc tttaatactt
     4681 ctaaatttgc tgggtccatt tctgctagag gtaatcttaa atctccaaca ttgaatccca
     4741 ataaattcat tgcagtctta actggtatag gatttacttc tataaataaa gctgcgatta
     4801 gagcatccat accaagttgt aattttctag aaccttcaat atcgccttca aagaatttag
     4861 ctaccaaatc gtgagtttct tgagggcaaa cattagcaag tactgatata actccttgac
     4921 ctcctaaaga taataaagga agtatagtat catcattacc agagtagata gcaaagtcat
     4981 ctggaactaa actagctatt tctgcaacct gtcctaggtc tccactagct tcttttacag
     5041 caactatgtt atcaatttta gcaagttcaa ctataaggct aggacttata tttacacaag
     5101 ttcttccagg gacattgtaa agtataattg gaagtttaac actgttagct attgtttcaa
     5161 aatgacgctt aagtccagct ttattagctt tattgtaata aggtgttatt attaatagag
     5221 catctactcc taatttttca gcttcttgac ttaagttaac tgagtgcata gtatcattag
     5281 aaccacttcc agctataaca ggaactcttt tattaacttt ttcaacaaca tacttaatag
     5341 tagcaagttg ttcttcatca gtcatagtat tagcttcacc agtagtacca caagatacta
     5401 tagcatcagt accattagct atttggtatt caactaattc acctaatttg tcaaaatcaa
     5461 ctttgttatc ctttgtaaat ggtgtaacta gagcaacagc agaaccttta aatatcatat
     5521 aaaatcaatc ccctcttttt tatttttgtg ttttatatta cagttacttc taatgcttgt
     5581 ttttgagtta ctattaatat tttacaaaat tatatattta tacaagtttg tatattatac
     5641 taaatcgtat gataaagcca tttcagctat ttgaattgcg ttagttgcag caccttttct
     5701 tatattatca gcaacaaccc aaagatttac accattatca acactaaaat ctcttcttat
     5761 tcttccaaca aacacttcat ctctatcatc aaattctaca gcagttggat attcgttttt
     5821 agcaggatta tcaactacta caacacctgg agcttcttca agtgttttta ctaaatcatc
     5881 taactcaaat ggattgttaa actcaacatt tatagattca ctatgaccat tttttactgg
     5941 aactcttaca gtagttgcag ttatttttaa atcgtagtta tcaagtattt tcatagtttc
     6001 atttaccatt ttcatttctt ctttagtgta tccattttca gtgaatgaat ctatatgagg
     6061 aagacagttg taagagattt gatgagggta gaatccagta ggattaccgt tcattccttt
     6121 ctctaaatct tcaacacctt taacaccaga accagataca gcttggtaag tagagtaaac
     6181 aactctctta attttgtatt tatcatcaag agctttaagg ggaaccatag cttgtatagt
     6241 agagcagtta ggattagcta ttataccctt atgatttttt atggcttctg gattaacctc
     6301 aggaactact aaaggcacat ctggattcat tctccattga ctagagttat ctactactaa
     6361 gattccattt gaagcagcta ttggagcata cttttcacta atacttccac cagcagagaa
     6421 taatgctatt tgtatgcctc tatcagtaaa tgaagtttcc tttaattctt ctactatata
     6481 ttctttacca caaaacatta ctttagaacc agctgattta gaagaagaga ataaaaatag
     6541 attttctatt ggaaaatttc tttcttctaa tacttttaaa aatgttctac ctaccattcc
     6601 agtagcacca actattgcta catttacttt tttcatataa attcacccca attttaaatt
     6661 attaaaacat tattttattt tagttataca agtaagatat gttaatattt ttatatatcc
     6721 aaaaacttta atattatatt aacaacttta attatataca aaaaacgtac aaaggtatac
     6781 tttgtacgtc taatttatca ctcaaagtaa accctgtagc tcatcaccta caaaaataag
     6841 tgacagtatt acacatgtta tgtgcaatcc cagaaaatat tattgcaaga aatattttct
     6901 tcggctgtaa atcctttttc caatcattaa cacctgccag ctactattgg atactcttag
     6961 ttacagctac ctctacccta ggttaatagg atattaaaat ttgattattc taataataat
     7021 acatatttaa gaaaaaatca acttatttat ataaatattg ctaaaattta aatccaaaat
     7081 ttgtcaattc ttgttttaac acattaagat tagtttcttc catttcagct agaggaagtc
     7141 ttaaatcacc aacattaaaa cctaaaatgt tcatggcagt tttaacagga actggattta
     7201 cttctataaa taaagcagca attaaagcat ccatatcaag ttgtaatttt tttgagcctt
     7261 caatatcacc ttcaaagaat ttagttacta aatcatgtgt ttctttagga catatgttag
     7321 caagtacaga gataacacca cttccaccta atgataatag tggtagtata gtatcatcat
     7381 taccggagta tatagcaaag tctttaggta ctaatttagc tatttctgca acttgagcta
     7441 agtctccact tgcttccttc acagcaacta tattatctat tttagcaagt tcagccacaa
     7501 cagatggctt tatattcact ttagttcttc ctggtacgtt gtataaaata attggcaact
     7561 taacgctatt tgctattgtt tcaaagtgaa gtttaagacc tttctcatta gtcttattat
     7621 agtaaggagt tattattaaa agaccatcca cccctaattt ttcagcttct tgactcatat
     7681 gaacagacaa catagtattg tttgaaccag tacctgctat aacaggtatt ctcttattaa
     7741 ctttttcaac tgtatatttt attacagcaa aaatttcaga ttcactcata gtagtcgcct
     7801 cacctgttgt tccacaaaca actagtgcat ctgtgccatt ctcaatgtga tattcgatta
     7861 attcacctaa tttttcaaag ttgacattat tatcctctgt aaatggggta actaaagcaa
     7921 cagcagaacc tttaaataac atataatcaa atctcctttt ttatctaaat ttaatttttt
     7981 aagtacaata ataattatag tacaaattaa ttaattttaa taggtatagt taatatctta
     8041 actgtttaaa taagatattt atattatatt aatattagaa aaattttaaa taaatgtttt
     8101 attctcaaaa tatgcatatt aatttaataa aatataccaa aatggaaata aaaaataaat
     8161 tttatgagaa tttaataact aataattaat atatttaggt tataataaat atgtgcgtaa
     8221 aatctaacaa taagcaataa taccgtaaat ttaggaggaa taatatggat actttaaagg
     8281 aactttttaa aataggaagt ggaccttcaa gttctcatac tatgggacca caaagagcag
     8341 cagagagatt taagaatgaa aacccagatg cagaaagttt tagagcaata ttatatggta
     8401 gtttggcagc tacagggaaa ggtcacttaa ctgactatat aattgaaaaa actatagcac
     8461 ctaaaaaagt cgaaattgtt tgggaagaag atattataaa agattttcac ccaaatggga
     8521 tgaagtttga agctttagat aaagataaaa atgtaacagc tgaatggact gtatattctg
     8581 taggtggagg gactatagca gaagaaggtc aaagaaacag taaaagcaat agtatatatc
     8641 acttagatac tatggatgag atagtaaaat ggtgcaaaga aaataataag actttggtag
     8701 attttgtgtt ggaatgtgaa ccaaaagaca taaaagatta tataaaaact ataaaagatg
     8761 ctatgagaaa atctattgat gatggactaa gtacagatga aataatacca ggcaagttat
     8821 tattaaaaag aagagcaagt acattttata atgcatataa aaaagataag agcttctcaa
     8881 cacttgtata tgcatatgca ctagcagctt cagagcaaaa cgcttctggt aatataatag
     8941 ttacagcacc aacttgtggt tctgctggag taataccagg aatattcttt gcaatgcagg
     9001 atttctataa ttatgatgac gaaaaaataa ttgaagcact acttgtagct ggaataatag
     9061 gtaatataat aaaaactaac gcttcaatat ctggagcaga ggtagggtgt caaggtgaag
     9121 ttggagccgc ttgttctatg gcagcagcag cagtagcata cttaaaaggt ggaactatag
     9181 accatattga atatgcggca gaaatagcac ttgaacatca tttaggaatg acttgtgacc
     9241 ctgtatatgg atatgttcaa attccatgta tagaaagaaa tgcaatggca gctcaaagag
     9301 cttatgatgc agctaattat gcattattaa ctgatggctc acattctgtc tccttagacc
     9361 aagttgttga aacaatgaaa gaaactggta tagatatgat ggataagtac aaagaaacag
     9421 ctaagggtgg acttgctaaa catttcttct cttgctaaaa ataaaatttt ccaataataa
     9481 ttcctctgtt ttgatttatg aaacagggga attattatta atggtgaatt atttaataat
     9541 gcaaaatatt tttatattca atttagcttt ttatttttaa ttagtataac atgctatata
     9601 atattttaat tataaagaaa tatagaaata ttttttaata aattagaaat tggaggagat
     9661 tacatgagag aggcatcata tttacaagaa caactttata aacataggaa gtcattaaat
     9721 cctattgcag agattgggag aaaagagtat aagacttcaa aatatataag agattatctt
     9781 gataaattag gaattgagta tgaagtttat ttagaaacag gtatagtagg tgtaatagaa
     9841 ggtaaaagac ctaaaaaaga tattgctttt agagctgaca tagatggtct tcatagtgaa
     9901 gaaggggtaa aacatttatg tggtcatgat ggacatgcta ctatattact tggattaatt
     9961 gagtatttaa atgataataa agaagactta aatgataata tagtatttat attccaacca
    10021 gcagaagaag gtcctggggg agcaaaagct ttaatagatg aaggagtgct tagaaaatac
    10081 aatattgatg aaatatatgg gcttcatata tatccagagt taccagaagg ttatgtaggt
    10141 acaagagagg gctattttat ggctcaaata ggagatattg acatagaaat agtgtctaaa
    10201 agtggacatg gggctatgcc acaaaatggt attgatggta tagtgatagc ttcaaacttt
    10261 gtaagctcac ttcaaactat agtgtctaga aatataagcc caatagacaa tgcagtgttg
    10321 actattggta gaattgaagg gggagctaga agaaatataa tagctgaaaa tataaagatg
    10381 gaaggtacta tgagatgttt cagcccagaa gtgtatgaga aaatgaagct tagagttaga
    10441 gaacttgcta gagggtttga attggcttat aactgtaagg ttaatataaa tattatagat
    10501 gattatattg ctgttaaaaa tgacaaggat ttgtatcaag agtttgtaga ggctattgga
    10561 agtgatactg ttgtagagtt agagccactt atgatatcag aagatttttc ttattatcaa
    10621 aaagaagttc caggattatt ttttatgttg ggttcaagaa acgaagaaaa aggatttgta
    10681 aatggtcttc ataatataaa ctttaatttt gatgaaaaga tatgtgtaaa tgcattgaat
    10741 gtttattcaa aattattaaa atacaaagaa gcaatagatt aggaagataa gttataaaga
    10801 ttaaaaagca taaataccta aatttaaaaa aagtgtatta ataaataccc cttacatatt
    10861 tgtataaatt tattttaatt gacaaaaata caaataagaa ggggtgattt ttttgaaaga
    10921 aaaagtacaa agagaaaaga agataactaa tggcaagaat actaaaatat tgacaaatga
    10981 tgatataatg aaatatgaga tagcctcaga actaggtctt atggataaag ttggtgaacg
    11041 tggatgggca ggacttacag ctaaggaagc aggaagaatt ggaggtatgc ttacatcacg
    11101 caaaaagagg cttaaaaaga ttgtggagga gcagaataaa aaggatgagt caatatagtg
    11161 attttgcgtt tgtatatgat gaacttatga atgaagttga ttataatggt tgggttaaat
    11221 atatagaaga tataatagat agtgaaggtg taaaagtaaa aaatatattg gaattggcat
    11281 gtggcacggg taatcttaca attccattga ctaaaaaaaa ttacgatata gctggtatag
    11341 atatatctga tgagatgctt agtgtagcaa gagaaaaggc agaaaaagaa ggagtagaat
    11401 tagttttact tcaacaagat atatcagagc ttgattttga aatatctgat ttggactgtg
    11461 tactttgtgc ttgtgatgga tttaattata taacttatga tgatgatttg gaaaatgtat
    11521 tttcaaagac atatgaatta ttaaagaaag atggaatatt catatttgat ataagttctt
    11581 tttataaatt agccaacata ttgggaaata acatgtatgg agaaaataga gaagatatag
    11641 cgtatatgtg gcaaaattac tttgatgaag aagagaatct aattgaaatg gaacttacat
    11701 tctttattaa agatgaatgt ggaaagtttg aaagatttga agagatacac caacaaagag
    11761 catatactga agatgagata gttgatatgc ttgaaaaatc tggatttaag gatataaaat
    11821 attatgggga ttttacattt gacaaaccta gtgaagaaag tcaaagaatc ttctttgtat
    11881 gtaaaaaata agatattctt gactatataa gataattatt aaaaataacc atgatataaa
    11941 aatatataaa atgcagtttt atagattaaa agtaaagtga tgatatttta tgatttttat
    12001 tttctaataa ttataaagtt ttatagataa aaattaaaat ataataacta ttgcttagta
    12061 aaattaaaat aaaatgggtt ttgcccaagg ttaagataga taagtattta tagattaaaa
    12121 ttaaggtagt aatataataa aaataagaca attaacggga catggaggaa tattaatgag
    12181 agattatgta ctaagagcga catcaggaaa tggtcaagtt agagcatttg tcgcaactac
    12241 tagaaataca gtagaagaag ctagaagatt acatgaaact acaaaagtag caacagcagc
    12301 acttggaaga acattaacag ccacatctat aatgggatta atgatgaaaa atgatagtga
    12361 taaattgact gtaataataa aaggtggagg tccaattgga actattatcg ccacatcaga
    12421 ctctaaggga atggtaaaag gatatgtagg aaatccacaa gtagaggttg aagactatcc
    12481 aaatggaaag ttaaatgtag cagcagcagt aggtacagaa ggtgttgtaa aagttataaa
    12541 agacttaggt cttagagaac catataatgg gacttatcca ttggtaagtg gagaaattgc
    12601 agaagacttt acttattatt ttgctgtatc agagcagact ccatcagtag tagctttagg
    12661 tgttcttaca aaagaagatg aagtagagtt tgctggagga tttatagttc aattaatgcc
    12721 agatgcagaa gaagagacta ttgctaagtt agaggaaaat gtagctaaat taccttctat
    12781 aactaacatg ttaaaagaag gaaaatcacc agaggatata ttaaatattg ttttggatgg
    12841 tttggaacct aaaatacttg atacctgtga agttgggttt atgtgtgaat gttcaaaaga
    12901 aagagtaaaa actgctttgg ttgcaattgg taaaaaatca ttggctcaaa taatagaaga
    12961 agataaaaaa gcagaagttg gttgccagtt ctgtaataag aagtatatgt atagtgaaga
    13021 agagttgctt gaaattttaa aggaaatgta gactataaaa atagttgtat tgatataagt
    13081 aatgttatag taatatagta aagtcgtatt gatatagtaa ttaaagtata atttattaat
    13141 aatagacata tttatattaa aaaataagtt taataaattt ttaagcttaa taaattttaa
    13201 gaattaagaa gctttctggc tgtaatgttt gcaatgttgc agttagaagg cttttttatt
    13261 ttattatagt tcgtgtcaaa gattgtatga aacaataaaa tattgtaaaa taatttgtat
    13321 atttagacct gattgacaca aaggtttata agatatttga gttgattata cgaaattcta
    13381 tgaagtagta accctgtgac tttaatcatg ggaggttcag aagaaggaga aaattttaaa
    13441 tatggaaagt aaaacgaagt acataggaat aagtatatta atattattga tgatattttt
    13501 aattgcaaag aattttaacg gaaaacttga aatacctatt cctgagagta atagtagaaa
    13561 atttaaatca gcagcagtgt tttatcctca acatcaagat gatgaggttt tatggggagg
    13621 aagtgccatt gtagatgcta taaaacagtg tggtgttgat aatgtatatg tggtattagt
    13681 atcagatggt tctggagtta acgttttcaa agctaacaca aaatttagaa atttaactag
    13741 gaagcaaaag gaagaactta gaaataacga gtttaaatca gctttgagag agcttggagt
    13801 gaaacctcaa aatgtgatta tacttgcaga tatagataaa aaggaaggca cacattatga
    13861 attaatggaa aagactatat tggattttga acataagttt aaaggcaatg tgacccatat
    13921 agcccatcac tataagtatg atgaccatat aatgcataga aagaatgggg aagttttaaa
    13981 gaaacttaaa cgtgaaggta agataaaaga tgacttgtat tttgttaagc caaaatatgt
    14041 aaagtatata ccaagaaatg aaagagctat ctataaagca atggattttg atgactacaa
    14101 taaggttaag agtgcttgtt atgaatataa ggttgtaaat tcaaagcgta aaatgtatgg
    14161 tataggatat atttcggcac atagttattt tgaacatctt ctaaatgacc ctgaacttac
    14221 gtctgtatta agtgaagatt aatttcttat aagtagtaag ttatgtttga taagttgatt
    14281 ggaattttct atagttttga aggtattgga aatttagttt tatttaaaga tttaaagata
    14341 gaggttttta gtgtttaaaa ttttaaagtt aagttgaata attgtataga attatttgtg
    14401 aaagaagaaa cagtgtaaaa catagtatct tatttatggt tttaaaatca taggcaagat
    14461 acttttttta tgtaactaat accattttga gattattaac ttaagaattt tatataattt
    14521 aacactcata tataatgatg atatttaggt agaatagatg taattacgct taaataaaat
    14581 aactgtattt tttataaaaa tccaaaccct acactatact accagataaa atctacatgc
    14641 tataatttga cctgtgttta taaaaaaagg agtaatctaa taaaatagta tttcacttat
    14701 gtataactaa caactattat tacgattttc tctaaataat ttaaagaaaa ataatgcagg
    14761 aggtaaaata tgaacaatga atttataatt ttaaaaggtt gcaaagagaa caatttgaac
    14821 aatatttcct taaaaatacc aaaaagaaaa ataaccatat tcacaggggt gtcgggttct
    14881 ggaaagtcat ctattgtatt tgagacaatt gcaaaagaat cacaacgcca actaaatgaa
    14941 aggtttagta catttgtaag aagtttttta cctaagtatg gagaggttaa agctgattgt
    15001 atagaaaatt tgtcaacacc aattatcata gaccaaagta gacttggtgg aaattcaagg
    15061 tcaactctag gtactattac agatgtaaat tcctttttaa gagctttata ttcaaggttt
    15121 ggtagtgagt atataggtaa tgccaatatg ttttctttta ataatattga tggtatgtgc
    15181 ccacactgtc atgggcttgg aaaaaagcta gttcctaata tgaaccaaat tcttgatatg
    15241 aataaatcat taaatgaagg tgctatattg ctttcagggt ttggagtggg ttcttggcat
    15301 tggaatatat tcgctgaatc aggatttttt gatattaata agaggatatg tgattattca
    15361 gaagtagaat tggaaaagtt tttgcatggt aaagcagaaa agattaagat agaaaatgct
    15421 ggtcaaacta acatgacata tgagggatta atggttaaat ttaatcgcct atacttaagt
    15481 agagagggag aaatatctga agctactaag aaaaaattga gtaaacttct tatagaagat
    15541 agatgtccta tatgtgatgg aagaagactt aatgaaagag tatataggtc tttaattaat
    15601 ggttataata tagcagattt aactagtatg caaattgatg aattggctga agttattaaa
    15661 agtatagatg agccagaagc acaaccttta attaaaggaa taatagaaaa gttaaacagt
    15721 atcatagata taggtcttgg atatttaaca ctagatagag agacatcttc tctatcagga
    15781 ggggaatctc agcgtataaa gatggttaag tatttaaata gtaatcttgt tgatttaatg
    15841 tatatttttg atgaaccaag tgtaggactt catccaaaag atgtgtataa attaaataat
    15901 cttttaaaaa aattgcgtga taaagggaat acaattatag ttgttgaaca tgacccagat
    15961 gttattaaga ttgcagacca tattgtagat gtaggaccaa aggcaggtaa gtatggtgga
    16021 gaagttgtgt atgaaggcag ttatgaaaat ctacttacaa gtggtacttt gacagggaat
    16081 gctttaagta aatttcttcc tattaaagaa agtgtaagag aacatagtgg atatttagaa
    16141 gttaaaaatt gtaataaaaa caatttaaaa aatgtatcta ttaagatacc taaaggtgta
    16201 ttgactttag ttactggagt agcaggagca ggtaaaagta cattgattaa ggatgaattt
    16261 ttaaagcaaa atcctagtgc tgtacttata gaccaaagcc ctgtttcagc aaattcaagg
    16321 tcgagtttgg caacatatag tggaattatg aacaacataa gaagcatatt ttcaaaaaca
    16381 aatggcgtaa atgcttcttt attcagttca aattcagaag gagcttgtga taactgtaag
    16441 ggttctggaa tagtagaaat gaatcttgct tttatggaga gtataaagag tacctgcaat
    16501 atttgtgaag gtaaaaaatt taaaaaagaa gttttagaat ataggtttca agggaagaat
    16561 ataattgaag ttcttgaaat gtctgtttta gaggcaattg agttttttaa tttaaaacag
    16621 attaaaacga aattacaatg tattgaaaaa atgggtattg gataccttac tttaggacaa
    16681 actcttgata ctttgtctgg aggagagtgt caaagattaa agcttgctag tgaattaaat
    16741 aaggaaagtt cagtgtatat attagatgaa ccaacaacag gtcttcatat ggctgatatt
    16801 gagaatttta taagtataat tgaagagatt gtagataatg gaaatacggt cataataatt
    16861 gaacacaata tagatgtaat taaaagagct gattggatta ttgagctggg acctgaagga
    16921 ggaacaaaag gcgggagagt aatatttgaa ggtattccaa aacaattatg taacaataaa
    16981 atatcattaa cagcaaaata tatagtataa taaataaaat ataataaaat tataatg
//
LOCUS       DAELTA010000084        16818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.084, whole genome shotgun sequence.
ACCESSION   DAELTA010000084 DAELTA010000000
VERSION     DAELTA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.084"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            106..1467
                     /locus_tag="KPG13_002278"
     CDS             106..1467
                     /locus_tag="KPG13_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0985989.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            1555..3444
                     /locus_tag="KPG13_002279"
     CDS             1555..3444
                     /locus_tag="KPG13_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0985990.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            3459..3914
                     /locus_tag="KPG13_002280"
     CDS             3459..3914
                     /locus_tag="KPG13_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0985991.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            3973..4290
                     /locus_tag="KPG13_002281"
     CDS             3973..4290
                     /locus_tag="KPG13_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0985992.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            4315..5577
                     /locus_tag="KPG13_002282"
     CDS             4315..5577
                     /locus_tag="KPG13_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0985993.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            5682..6746
                     /locus_tag="KPG13_002283"
     CDS             5682..6746
                     /locus_tag="KPG13_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0985994.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            6800..7978
                     /locus_tag="KPG13_002284"
     CDS             6800..7978
                     /locus_tag="KPG13_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0985995.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(8169..9227)
                     /locus_tag="KPG13_002285"
     CDS             complement(8169..9227)
                     /locus_tag="KPG13_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG0985996.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            9294..9665
                     /locus_tag="KPG13_002286"
     CDS             9294..9665
                     /locus_tag="KPG13_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG0985997.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            9702..10376
                     /locus_tag="KPG13_002287"
     CDS             9702..10376
                     /locus_tag="KPG13_002287"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG0985998.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            10625..11464
                     /locus_tag="KPG13_002288"
     CDS             10625..11464
                     /locus_tag="KPG13_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG0985999.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            11708..11804
                     /locus_tag="KPG13_002289"
     tRNA            11708..11804
                     /locus_tag="KPG13_002289"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11742..11744,aa:Sec,seq:tca)
     gene            11954..12661
                     /locus_tag="KPG13_002290"
     CDS             11954..12661
                     /locus_tag="KPG13_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG0986000.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(12803..13639)
                     /locus_tag="KPG13_002291"
     CDS             complement(12803..13639)
                     /locus_tag="KPG13_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986001.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            13827..14732
                     /locus_tag="KPG13_002292"
     CDS             13827..14732
                     /locus_tag="KPG13_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG0986002.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            14741..15925
                     /locus_tag="KPG13_002293"
     CDS             14741..15925
                     /locus_tag="KPG13_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0986003.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            16040..>16818
                     /locus_tag="KPG13_002294"
     CDS             16040..>16818
                     /locus_tag="KPG13_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0986004.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGII"
BASE COUNT         6693 a         1665 c         2868 g         5592 t
ORIGIN      
        1 aatagtaaaa aagttttgtc ccttgaaatg acataaagta taaggagtta ttaaataaga
       61 taaactatga tatataatat aaagttctaa attggagtga taattatgga gttgtttaag
      121 gcagaaagca aaaaatataa aaaagaattt ttaaaattat tggagcaatg ggttagtata
      181 ccttcatttt atgatagaaa aactgtatct aaagacatgc cttttggaaa aggagtatat
      241 gatgcattaa actggtttga aaatctaggt agagaaaaca atttcaaagt aaaaaatata
      301 gataatcatg ctgttcaaat tgaatatgga aatggtaaag agtatgtaga tatatttgga
      361 cattgtgatg ttgtaaatcc aggtgaagga tgggatagtg aaccatttaa actaaatatt
      421 ataggtgata aattagttgc tagaggtgta tctgataata aagggcctat gatagtgaat
      481 tttttagcac taaaaatgat aaaggattta gatattaatt taaaaagaaa ggttagatta
      541 atagctgggg gaaatgaaga gagtggattt aaatgtataa agcattatta tagcaaagag
      601 ccatatggtg tatgtggatt tactccagat gctaaatttc ctgtattaaa tggagaaaaa
      661 ggtggagcaa taataaagtt aatttcaaat atagacgata aatctttata tataagtggt
      721 ggaatagagt ttaatacaat accagataaa gtttatataa agaatgtaga aaaattgggt
      781 aaagataata tatgttttga tattaacaat atatcgataa attatgataa tggcaattat
      841 attgtgtatg gaaaaggtgg tcattcatct aaacccgaaa aatcaataaa tcctatatta
      901 gctacaatta aacttttgtc agaaaatatt gatgaaaaat ggacaaaaga tttatataag
      961 ttgataaatc aagataatat aaatggaaac ttatttggtt taaatattga ggggaaatgt
     1021 ggaattttat caatggtgcc aacaataata aatatagtag atggaaaatt agaagtagtt
     1081 ttaagcgtta gatatccaga aatattaacc attgaagata taataaaaaa atttaatttg
     1141 tatatggagc aaaataatat taataaattt gagcttattg gggaaaattt aaagcaagca
     1201 aattatatag atagaaattc aaaacttgta agaagtttgc atgatatata tataaagtat
     1261 tctggagatt taaaaaatga tgttagagta actagtgcag gaagttatgc ttctgaaatg
     1321 aataattcag taatatttgg ttgtgaattt cctgatggta gctttggcaa tgtccatagt
     1381 gcgaatgaat ttgctagctt agatagattt ataactgcaa taggtatata tgcagaggca
     1441 ataattacat tatgtaataa aatataaata caaaatgtcc cttatataga catattatat
     1501 atacaattaa aattttactt gatatataat tgaaatcaaa gagaaggtga tacaatgata
     1561 actaagaaac atgcaataat agtaaagagt ttaagcaata aagatggata tatgacatca
     1621 aacgaattag ctatcaaatt ggatgtgtct actaaaacta ttaaaagata tatagcagat
     1681 ttaaatagtg ttttaagcaa atatgatttg gtaatagctt cttctagagg tataggatac
     1741 aagttaagcg gttctaaaaa caatatagcc agagctgtta aagaagcaaa taaatatatt
     1801 gatggttttt tagatgactc tgaagagtca agaatgtcaa atataatatg tatgcttatt
     1861 aatagaaatt atatgagtat agaagcaatg gcagaagaat taaatctaag tatagccgct
     1921 ataaataaat tgtctagtaa gcttaaaaga aaacttgaaa agtatgactt agttataaaa
     1981 tctaaacctt actatggaag ctatatacat ggtgaagaaa ttaatataag gcaattaatt
     2041 actgactatg caataaaatt agatgaaaaa agtaaagtga aggtgttttt agatgacatt
     2101 agtgaaaacg atatacactg tatagaaagt attttagaaa agcacttaag agaaaaagat
     2161 acaataattt cagataaaga ttttaatctt ttgctttcaa aaataatcgt ttctgtattt
     2221 aggagtaaga gaggtcatag taacaatata aacttaatgg atacaagtta tagatttcat
     2281 aactactact ttatagaaaa tctaatgaaa gaaatatctg ataaaattgg atttaaactt
     2341 attgaagatg aagtaattta tatttcaaat tattctggtg taatagccta taaaggtaca
     2401 caaggaagaa aaaacacaag tgaaattgag gaacgaataa gtagtgttat aagtagtgca
     2461 cttcaagata tattccttat ttcaggtagt gattatacaa aagatgatga gtttatggtt
     2521 gcaatctctg accatataaa gagattttta aatagagcta gagctaatgt aaaatcaaat
     2581 aatcctttac ttcatcaaat aaaagagaag tttcctatag cttttaattt agccgtgttt
     2641 ttatcaaata aactagaaac tgagtttaat ttaaaacttg atgaagatga gcttggatat
     2701 atagctatcc actttgctgc ttcaaatgaa agaatgaaaa aaaatactag taagaaaatt
     2761 tgtatagttt gtcattatgg gataggaaca ggacaactta tttctgaaaa gctaaaacag
     2821 aatataagtg atttaagtgt tgtaggtgtt tatccagtta gatatctcga tatggctata
     2881 agtcaagatg ttgatttgat agtatctacc gttgaattaa aaggatatga aaaacctgtc
     2941 ttatatatag aaaatatatt tgatgatagc ttaattgaaa acgtaaacaa agccttttat
     3001 gaaaaggaag aaagaagaaa gataataagc aacatgtttg atgaaaaagc ttttttcagt
     3061 ataaaggcta atactaaaga agaggtcata atttcacttt caaataaact aaaagaaaga
     3121 gattttatag aggaaagttc tataaaaagt ataatagata gagaaaatat ttcatcaact
     3181 gaaataggaa atctagtagc aataccccac acaatagtga agggagataa aaaatctata
     3241 ataggagttg gaatacttga aaatccaata atttgggata agcaagaggt tcagctagta
     3301 tttatggtat tttttaatac taaagaaaaa cataatttct cgatatttaa atacctttat
     3361 aattttataa aagatgaagg cggagtaaga ggaattatta aaatatgtga ctttaataaa
     3421 ttaatggcac tgataggtaa ttaaggagga aattaataat gggaacaaac atatttaaca
     3481 aagaatatgt atttttaaat gttgatgcta agtcaaaggt tgaagtttta aaatttatat
     3541 caaaaaaagc taaagactta aacttggcag aagatgagag cttagtatat gaaggtttaa
     3601 tggctagaga agaacaattt actacaaatt taggagaatc tatagcaata cctcacacta
     3661 aaaatgatgc aattgaaaat ccagcagtag tagtattgaa gtttaatgaa gatgttgttt
     3721 ggaatgaagg tgaggataag gttaaattag caataagctt acttatgcca ggaaaaagta
     3781 aagagaatat tcatcttaaa ttattatcat ctctttcaag aaaattaata aataaagaat
     3841 ttaaagattc tcttttaaaa agtgataatg ttgaagaaat atctaactta ataaatgaag
     3901 ctttaggttt ataattaaac actaaggtta taaaataaaa aaataattaa ctaaaaatag
     3961 gaggaatttg aaatgaataa aaaattagta gcattatgtg cttgtccaat gggactagct
     4021 cacactttca tggcagcaga agcaatagag caagcagcaa aggcattagg ttatgaagca
     4081 aaagttgaaa ctcaaggtgc tgatggtgta caaaatgagt taacaagaga tgatatatta
     4141 ggagcaacta tgataataca tgcagtagca ataacaccag aaggtatgga aagatttgat
     4201 ggttgtgaag tttatgaagt tgaattacaa gaagctataa aaaatgcaga aggtgttata
     4261 aaagaaatag aagaagattt aggaatataa taaatttatt gtggaggaaa tagaatggca
     4321 attaagaaaa aagttataaa cagttcaggt gcttctacaa caggaggaaa taatgtagga
     4381 aaatctactc catctagtcc aactcaatca aagggaaatg gaaaatggaa agaaataagt
     4441 aaacatataa tgacaggtat atcatatatg ataccagttt tagtaatggg tggacttata
     4501 ggggccttat ctcaattaat accatatgct atattaggac ttgacccatc tgtaggtata
     4561 gttgatgcta tgaactcagg agaatttaca gggttcaagc ttagtctttt aaatatagca
     4621 caattaatgt caaatttcgg atttactttg tttggatttg cgataccact gtttgcagca
     4681 ttttgtgcaa actctatagg tggaaaaaca gctttaattg caggatttat aggtggatat
     4741 atagctaata aacctgtagg tgtaccacaa ttcgttgatg gacaatggac tgaagttgtt
     4801 ccagttgcat caggattttt aggtgcaata ctaatagcat ttataatagg atattttgta
     4861 aaatggttaa ataaatctat aaaagtttct cataactggt tagcttttaa aacaacattt
     4921 ttaataccat taatagcgtc attagcttgt atggtattga tgatatttat aataactcca
     4981 tttggtggat taattaatga aagtatgaaa aacttcttaa cagcagcagg agcagctgga
     5041 gaatatgtat atgcaacagc tttggcagca gctacagcat tcgacttagg aggaccaata
     5101 aataaagcag caggattcgt tgcacttggt ttaactacag agaatgtact tccaataaca
     5161 gcaagaacaa tagctatagt tgttcctcca attggattag ggttaacaac attattagat
     5221 aaaagattag taggaagaag agtatatgat agacaattct atcaagcagg aaaaacttct
     5281 atattcttat catttatggg tatatctgaa ggagcaatac catttgcact tgagagacca
     5341 ggttttgtta tacctttaaa catagttgga agtgtaatag gagctattac aggtataatt
     5401 ctaggagcaa ttcaatggtt cccagagtca gctatatggg catggcctct agtagataac
     5461 ttatttggat atataatagg tatagcagtt ggtgctatat tcatagcagt aggaaatata
     5521 ttctatagaa ataaattaat aaaagatggt aaacttgttg tagattatat cgactagtat
     5581 ttaattttta ctatctataa ataactttgg ctggtaatat ggttttaatc aatattacca
     5641 gccttaccta tatgattaat taaaggcggt ggagataaga tatggataag atagaaaaag
     5701 taagagagta ttttagagaa tatgatatag atggatttct tataaatagt cctacaaaca
     5761 agttttatgt tggaaattta tttagctctt caggttatgt ttttataacg aaagaatcac
     5821 aatatataat agtagatttt agatattttg aagaaattaa aagaaaatct agtttgttta
     5881 atgtagtatt aatggacaaa actagaactc attttgatat aattaatgat atttgtagag
     5941 agcagaatat aaaggaaatt ggatttgagg gaaatgaagt atcttttgat ttatatagaa
     6001 gtatgagtaa taagttaagt gcaaccttaa aatcagttga tttatctaca ttaagggaaa
     6061 caaaaaatga agacgaaata aaatatataa aaaaagcttg tgaaatagtt gatgctacat
     6121 tttatcatat agtagacttt ataaaggttg gtatgactga aaagcaagtt gaaaatgaaa
     6181 ttgttagaat tataaaagat ttaggtggtc aaaaagaatc tttcgataca atagttgcat
     6241 ctgggcttag aggagcatta cctcatggaa aagcaagtga aaaagttata gagtatggag
     6301 attttgttac ctttgacttt ggtgctaagt ataataatta ttgttcagat attaccagaa
     6361 ccatttgtat gggtactata aataaagagt tagaagaaat atataatata gtaagaaaag
     6421 ctaatgaaga gtgtataagg gttttaagac cgggaatgac tactggagag atagataaag
     6481 ttgcaagaga cataattggt tcatatggat atgctggtaa ttttggtcat aatctgggtc
     6541 atggtgtagg aattatggtt catgaatatc ctgcattagc tcctgaatca aatgaagtat
     6601 taaaagaagg aatgattgta actatagagc ctggtatata tgttccaagt cttggaggcg
     6661 taagaataga agatgatgta cttataacac aagatggatg tatgagattg actacatcta
     6721 ctaaggattt aatagttgta agctaaaaat aaattagata aatattttat aacaataaga
     6781 aaagaagagg taaatttaaa tgtataattt tgatgataaa ccagatagag taagtgaaaa
     6841 gtgtagaaaa tgggatttaa atattataag agataaattt ggagatatta gagaagattt
     6901 tataccaatg tggattgctg atatggattt taaaatacca acagaaatag aaaacaaatt
     6961 tattgaagct gtaagaagag gtgtatttgg atatacatac tgttatgatg aattttatga
     7021 ttcagttatt ggttggcaaa aaaatatgca tgaggtaaat gtagaaaaag aatggattac
     7081 attaacttat ggaactgtat caacacttca ttatgtagta caagcttttt gtaagaaagg
     7141 tgatagtata atattaaata caccagttta tgaccccttt gaatcatcag caaaaaaaca
     7201 aggtgtaaat gtaatttgta atacattaga tgttgtaaat gatagatatt atataaattt
     7261 tgataaatta gaagttcaaa ttaaagaaaa taagcctaaa ttaatgatgt tttgtacacc
     7321 acataatcca tctggaagga tatggacaat tgaagaaatg acaagggtag caactatatg
     7381 taaagaaaat aatgttattt tggttgcaga tgaagtacat gcagagcata ttcattatgg
     7441 tacatttaat tcaatactta agattggaaa agagctttta gaaaatgtta ttctattgac
     7501 atcaccaaat aaaggattta atcttggtgg attaaagact tcttattcaa tagttataaa
     7561 taaggatata agggacaaat ttagaaataa attaaagcaa aattctataa cttctccaaa
     7621 tgtgtttgga ataataggat tgataaccgc ttataatgaa tgtcatgagt ggttaagtgg
     7681 tgtaaatgaa tatataaaat caaattatga acttttagaa acttgggtaa ataaatataa
     7741 taagataaaa ttgatgaaaa tggaatcatc ttacttagct tggatggata taagtggatt
     7801 aggtattagt gcaagtgagt ttacagataa attggctata gatacaggag tattattaga
     7861 agatgggagt cattttgtaa aagatggtga gaagtatgtt agaataaatt taggaactca
     7921 aaaggaaaat gtaattgaag ctttaaatag aatggattta tttttaaagt ctttataaaa
     7981 atttggttga attagtattt tatataaatt taaactatta atatggtata tacataagta
     8041 gaatttgttt tagattactt aagtatatac catattttat ataaaaatta aatttgcatt
     8101 agtcatgtga taactattta aattcatatg tattttgtat gtataataga tataattatt
     8161 atttaaactt attgtttatt taatttttca atagaaaaag atttatctgt aagtttaaaa
     8221 ttatctttta tacccttagt taagtatact ttcttatcct tagttatgaa tatagcatct
     8281 acattatcaa ttttattaat taaattcata cctttttcaa gaccaagacc aaatgtagaa
     8341 gtggataagg catcacaatt aatagattta tcagagatta ttgtaacact acttaaattg
     8401 ttttcaaaag gataccctgt aaatggattt aacatatgat gatatatttt tccgtttttt
     8461 tcgataaatc tttcataaat tccagaagta acaacagact tatttgtagt ttctatgttt
     8521 cctatggatt caccattttt actagttggg tcttgaatac ctattttaaa aggtttatca
     8581 tttttaccat tttctaaagt aaatatattt cctcctaagt ttactaaacc tttttttacg
     8641 tcctctgatt ttaaataggc tacaatttta tcagcagcat atcctttagc tatggcacct
     8701 aaatcaattt tcatattttc tttggttaat tttatagatt tatttttttc atccaatatg
     8761 atgttcttat aatcaataag aggcagaagt ttttgtatct cagaatcact tggaacttta
     8821 gctttatctg ttccaatagc ccataaattt gataatggac caattgttat gtcaaaatat
     8881 ccatctgata atttggaata ttgtatagct tcttttacga cgaaaaatgt atcatcagaa
     8941 actctgacaa attttttacc agcattatca tttattttag aaacatcact tcctggtata
     9001 tgagtgctca ttttgttgtc aatatatctt aaaattgaat cacattcatt taagatttta
     9061 tcacttttgg attcttttac attaaataca gtaacttcgt ttactgtacc aaggtagtag
     9121 ctagttttac tatatttagt attctggctt gaactcctaa aaaatgaaaa taaaattaat
     9181 gctaaaatca atatcacaaa aataaatgtg atttttttct tattcatggt atattcctcc
     9241 tatgaaattg tgttattatt ttattataac ataatttcat aggaggacaa aaaatgaaga
     9301 aaaaagacat tcttcttatt ttaggcattt tggttgttat tacagcatgt tatgggataa
     9361 taaatgttat aaattctaaa aatgctggaa atattgagat ttatgtagat aataaattgt
     9421 ataaaactgt ttcaataaat gccaaagaag aatttaaaat agagaataga ggggggtata
     9481 acatagtaaa gattcatgat aaaggggttg agatagtaga tgcttcatgt cctgataagg
     9541 tttgcgtaca tactggattt ataaacaagc caagtcaaag catagtttgt atacctaata
     9601 gagtgagcat aaaaataaat acaaatgaaa agaatgataa tcaagaagat ataatttcta
     9661 aataaaataa taaaaataat tgaaaggaat tggtgataat tatggttaac ttaggaaacg
     9721 attgggatga attattgaaa gaagaatttg aaaaagatta ctacttaaac ttaagaaaat
     9781 ttcttataga agaatataaa acacgccaaa ttttcccaaa tatgcacaat atatatgaag
     9841 ctttaaaaca cacttcatac aaagacacaa aagtcttaat attaggacaa gacccttatc
     9901 atggagataa tcaagctcat ggtttagctt tttcagttca accacaagtg aaaactccac
     9961 catctttatt aaatatgtat aaagaattaa aagatgattt aggttgtttt atacctaata
    10021 atggatattt aatgccttgg gcagaccaag gagtgttact tttaaatact gctcttactg
    10081 ttagagcgca tgaagctaat tctcataaga acaaaggatg ggaaattttc actgatagag
    10141 taatatctat tttaagtgag cgtgaagacc cagttatttt tgtactatgg ggaagtaatg
    10201 cacgtaaaaa agtagagttg attgatacaa gtaaacatta tattttggaa gcacctcatc
    10261 caagcccatt gtcagcaagt aaaggctttt ttggatgtaa acacttctct aagataaatg
    10321 aaattttgaa aaagctagga aaagaaccta ttaattggca gatagaaaat atataaaagt
    10381 aaaaggtaac aaaatgataa caaaacaaca ataaacaatg acattgttgt aactaataga
    10441 aatatgctta aatagttgaa aatactatgt atgcatattt ctattttttt gtcaagttga
    10501 caactcttga aaaaatagtt acaatttagt tacacaaaaa acacaaataa aaaaagaaac
    10561 ttacaaagca gaattttatg aagcaaaata tatagtgtaa aaaatcttag ggaggaaatt
    10621 ttatatgaat tttaaaaaat tgagtaatgc taaagtctcg gtaatggcag ttgttttatc
    10681 agttggaata ttatcgggat actctgcgct taataaacaa gtaacattag tagtaagggg
    10741 agaagaaaga actatatcaa catttagttc taatgtagga gaccttttag aatctcaaaa
    10801 cattagctat gatgcaaatg atatagtaac tttagatgaa aatacaaagt tatctaatgg
    10861 agataaaata gaagtaattg atgtaaagga aaaaacagtt gtagaaacta aagacattcc
    10921 atttgaagtt acagttgttg aagatggctc tttattaaag ggtgattcta aggttaaaga
    10981 agaaggaaag tctggtaaaa gtgaattaat atataaagaa acttatcaca atggaaagaa
    11041 agtagaaaag gaatttgtga aaaaggttgt taaagctgag ccagtaaata aggttgtaag
    11101 caaagggaca aaggtagaaa tgcaagtagc ctcatcaagg ggagaaagtt caagaagaat
    11161 ggcttcatca tcatctagta gcagtagtag taacaatggt aaaaatatga gagtggtagc
    11221 aactgcatat actggtcaca gtataactgc aacaggaaca aaacctaagt ggggaacaat
    11281 agctgtagac cctaaggtta taccatatgg aactaaagtt tatatacctc aatttgacat
    11341 ggtgtttact gcagaagact gtggtggagc aataaaagga aataaaattg atatctacat
    11401 gaatgattca aaaacagttt ataactgggg aagaaaaact atagatatac gtatattaaa
    11461 atagttaaat aatttatatt tttactggaa attatatttt gattatatag agaagaaact
    11521 ctttatagat tctaaaaata taatttccag ttttttattt tggatatgta ataaaacagg
    11581 taaattttaa ccatgtatag ataaaagcta tttaaattat aatttactat catataaatc
    11641 tataaaaatt gttgcaaaga gtcaaaatat atgatattat agatttcgtt gagaaggatt
    11701 tcattatggg agtggatggg tgccggtgtg ccctctagtc ttcaaaacta gtatgagggg
    11761 ttaagagcct cttaggtggg ttcgattccc acgcattccc gccaatatgt aaaaggtatc
    11821 ttttaattaa aagatacctt tttaaatttg ttttaagata atttagtttt aatgatatat
    11881 aatattttgt attatataat gtaaatttta taatactatt attgttcaga taaaagtcta
    11941 ggagagtgaa aagatggaaa ataattttac aacgagaact agttttctag ttggagatga
    12001 tgggatagaa aaacttaata attcaaatat aatagtattt ggagttggag gagttggtag
    12061 ttttactgtt gaagctttag caagagctgg agtaggcaat attactattg tagattttga
    12121 tgatgtagat ataaccaata taaatagaca aataccagcc ctacattcaa ctgtaggaag
    12181 atacaaagtt gatgtcatgg aagaaagaat attagatata aatcctaata tcaatataaa
    12241 aaaaataaga agtctttata ataaagatac tagtgacgaa atactaactg aaagatatga
    12301 ttatgttgtg gatgcgatag acatggtaag ttccaaaata catctaattg aaacttgtga
    12361 aaaaaaagga ttgaaaataa taagttctat gggtatggga aataaattag acccaactaa
    12421 aatagtcgtt acagatatac acaaaacaag tacttgtcct ctagctaaag ttatgaggaa
    12481 agaacttagg gatagaggta tcaaaaagtt aaaagtagta tattctactg aacaaccaat
    12541 agaacttaag aagaaagtaa tgaatggtag aaaagtaaca cctggtagtg tatcatttgt
    12601 cccatctgta ggaggtttaa taatcgcatc tgtaatagta aatgaactat taggacaata
    12661 agttttaaat attttaattt ataaatttta tatacaaatt tgaaggctag aaggatgaat
    12721 ataaagcaat tttatattca tacttttagc ctagttattt tatattacct tattttatgt
    12781 agttgtagct ttatttttta gattatgtat ctatttcata attatatttt gtaaatataa
    12841 tttctccatc actataaaaa acaatatctc ctattctgtc tgttctataa attgatacat
    12901 ggttttgttt tagattatta ataacctccc tatgtggatg accatattga tttttatatc
    12961 cacatgatat tgcagctata tctggactaa ttttttttat aaattctaaa gatgaagaag
    13021 aattacttcc atgatgacct atttttataa aattaatatt atttaattta aaagaatgta
    13081 aaatttcttt ttcattttct tcttcagcat cacccataaa taaaaaagaa ttatcattaa
    13141 aagttaattt gaaaactata gaatttaaat tagattcttc ttgtatatat gatggactta
    13201 atacatatat atttatattg ttatctatgt ttaaaacatc atttttatag agatgctgaa
    13261 tagataagtt tttatccgta caagaattta ttagattttg ataagcctca ctatcagttg
    13321 attgttcagg catatatatg gagtttacat taaatttttt tataatatta tcaagacttc
    13381 ctatgtggtc tgaatctgga tgagttgcta ttataatatc aatagttttt attctttttt
    13441 gtctaagata actaataatt atattttcgc tatcttcatc acctccatca attaatatat
    13501 tcttatttgt gggtgtttga actaaaatac tatcaccttg tccaacatca atcatatgta
    13561 tagatagtag agattttttg tcacagccga ttaaaagtga tattataata agtattgata
    13621 aaaaaatttt atttctcaat aaaaaatcac tccattcatt acatgtttat ataaatttat
    13681 gatgggtaat ataagatata tatgacttat tatattttat attttaaaca atataaatga
    13741 aattaatacc ataataaaat tggagttttt tatatataca aaataaaatt tttaaaatta
    13801 agcaataaaa aaggtaggag gattttatgt ttttcaaaga tattgaacta aattcaaaaa
    13861 aagagttaga cccatatttt gatttagtag attatgaagc ttgtgagtac tgttttagta
    13921 ctttatatat gtggcaacat gtttataaga caggatatta tataggtgaa gattttgccg
    13981 ttttagttgg ggaatacgaa ggagatagct tctcaatatt accacttgct aaaaaagata
    14041 aacttccaga agttgtagat tttgtccttg aatatttttc taaaaataat aaaaaaatat
    14101 acttaagagg tattactact gaagtagtag agtttttaaa agaaaaatat ccaggtagat
    14161 ttgagtatat agaagaaaga gatttatttg actatattta tgatgcagaa agtttaagaa
    14221 ctctagctgg taaaaagaat caaaagaaga gaaaccatat aaactatttc ttaaaagagt
    14281 atgcaggtag atatgaagct aaattattag ataaagaaaa ctttgatgag tgtttagtac
    14341 taatgaagga atgggaaagt aataaggaag aaaataatga atttgatgaa agtatggatg
    14401 atgaattaat aggtatcaaa aagatattta accattatga catattaaaa gataaggtaa
    14461 aagtgtttgg tgtttatgta gatggaaaat tagaagcttt tagtatagga gagcttttaa
    14521 atccaaatat ggcattgatt catatagaaa aagctaatcc tgatataaga ggtctgtatc
    14581 catttataaa tcaacaattt ttggtaagtg aatttaagga cgttgagttt gtaaatagag
    14641 aagaagattt aggtattgaa ggattaagaa aagctaaact ttcttatcat ccttgcagat
    14701 ttgttgaaaa gtacagtgtt agggaggctt aatagactat atggagatta ggtatgctaa
    14761 agaagaagag atagaaagta taaaagagat ttggagttat tgttttaacg atacggagag
    14821 ttttatgaag tactatttta atgataaata taaatctgaa aatactgtag tagctttaga
    14881 tgaaggaaaa attatttctt ctcttcagct aaatcagtat aagttattac taaatagcaa
    14941 ggtatataat acatcatatg tagtaggagt ttctacactt ccagagggaa gaggagctgg
    15001 atatatgaac aaagtaatga aatttacttt aaacgaattg tataaaaaag ggcaattagt
    15061 ttctatattg atgcctattg actatagact ttatagaaga tttggatatg agcattgtta
    15121 cgaccagatt gaatatacta ttaacacaga tgatttaaaa aattttaagt caagtggaaa
    15181 aatgataaag tctaatttat cacaaattga tgatttgata caaatagata gaactttttt
    15241 aaatgaggtt aatggtaatg tgttaaaaga tgaacattat tatgaaaact tatttaaaga
    15301 gattcaaagt gaagatggat ttttatacat tcatgaagga aatgaaaaag atggatatat
    15361 agtttatttt cttcaggaag ataaaatgtt tgtcagagag ttattttata aaaatataga
    15421 tgcccttaaa tcaatgctta aatttatata taatcacaat actcaatgta agatagttac
    15481 tatatctact cctacaatag ataagataag atttatatta gacaacccta aagatagtga
    15541 tataaaaata aaacctttta tgatgggaag agttataaat gtaaagaagt ttattgaaga
    15601 tatagatatt gagaatgata ttaatagctc atttaattta ttaatagaag acaagtttat
    15661 agatgaaaat aatggtctat ttaaaatttc tatacagaat aagaaggtat ctgtagagca
    15721 gttagataaa aaaggtgcag aaaagcctca agaagatttt gatattaaat tagatataaa
    15781 taccttaaca cagttatcat tttcttatat agatgtaaat gaagctattt tcttaaatga
    15841 tataaaagat gtttcagaag aaacactaga aacacttaat tgtatttttt ctaaaaaaaa
    15901 taactatata aatgaatata tataggtaga gtacgttaaa agattttaaa aatcttttga
    15961 cgtatttttt tgtttagata acaatttatc ttgtttaaaa attttctatt tgaaaataat
    16021 ctaaataata tacttttata tgggagtgat tataattcaa ataagaagac cattattaat
    16081 tatattaatg tttgtggtag ctatatcatt tatcattaca aataacacaa aaaacgacga
    16141 agaacttaat gataagataa taactataca aggagttgtc aaaggaaaaa ttgaaaagaa
    16201 aagatacaat cagtacaagg taggggtttt tctaataaat gattatacaa aaaataaaaa
    16261 tttgaagatt ggtcagaaag ttaatattac aggtaaattc aaaagtttag ataaaatgaa
    16321 gtatgatgat tttgattatg gaaggtatat aaaaagtact ggatataaag gtatagtgta
    16381 tataaataac tataaaatta ttgataaaaa taaactttac tctttaatag gggaaataaa
    16441 gttttatatt agtaaaactt ataggtactt atataagaaa aactcagatt ttataaattc
    16501 tatactatta gcagaagttg aaaatttgac tgaggaacaa aaagaaattt ttactagaac
    16561 tggaactagt catgtaatat ctatatcagg tcttcataca ggaatattat gtgtaattat
    16621 atcctttcta ttaagaggta taaataaatt gtataaatta ttaatactgt cgatttttat
    16681 aactttatat tgtataatgg taggtgcgtc tccatccata attaggtcta tagcatttgt
    16741 tatgattttt tatttatcaa tatttattga tagaaagaaa gatggaatat cggcattgtc
    16801 tctaattggg ataatttt
//
LOCUS       DAELTA010000085        16709 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.085, whole genome shotgun sequence.
ACCESSION   DAELTA010000085 DAELTA010000000
VERSION     DAELTA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.085"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            302..1477
                     /locus_tag="KPG13_002295"
     CDS             302..1477
                     /locus_tag="KPG13_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0986005.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            1512..3050
                     /locus_tag="KPG13_002296"
     CDS             1512..3050
                     /locus_tag="KPG13_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0986006.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3082..4284
                     /locus_tag="KPG13_002297"
     CDS             3082..4284
                     /locus_tag="KPG13_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0986007.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(4451..5050)
                     /locus_tag="KPG13_002298"
     CDS             complement(4451..5050)
                     /locus_tag="KPG13_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0986008.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            5220..6578
                     /locus_tag="KPG13_002299"
     CDS             5220..6578
                     /locus_tag="KPG13_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986009.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            6571..7203
                     /locus_tag="KPG13_002300"
     CDS             6571..7203
                     /locus_tag="KPG13_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG0986010.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            7532..8689
                     /locus_tag="KPG13_002301"
     CDS             7532..8689
                     /locus_tag="KPG13_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986011.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(8836..9471)
                     /gene="lexA"
                     /locus_tag="KPG13_002302"
     CDS             complement(8836..9471)
                     /gene="lexA"
                     /locus_tag="KPG13_002302"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG0986012.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(9575..10702)
                     /locus_tag="KPG13_002303"
     CDS             complement(9575..10702)
                     /locus_tag="KPG13_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0986013.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(11013..11858)
                     /locus_tag="KPG13_002304"
     CDS             complement(11013..11858)
                     /locus_tag="KPG13_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986014.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(11888..12259)
                     /locus_tag="KPG13_002305"
     CDS             complement(11888..12259)
                     /locus_tag="KPG13_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0986015.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(12412..12672)
                     /locus_tag="KPG13_002306"
     CDS             complement(12412..12672)
                     /locus_tag="KPG13_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG0986016.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(12861..13805)
                     /locus_tag="KPG13_002307"
     CDS             complement(12861..13805)
                     /locus_tag="KPG13_002307"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG0986017.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(13826..14680)
                     /locus_tag="KPG13_002308"
     CDS             complement(13826..14680)
                     /locus_tag="KPG13_002308"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG0986018.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(14695..16056)
                     /gene="accC"
                     /locus_tag="KPG13_002309"
     CDS             complement(14695..16056)
                     /gene="accC"
                     /locus_tag="KPG13_002309"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG0986019.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(16072..16524)
                     /gene="accB"
                     /locus_tag="KPG13_002310"
     CDS             complement(16072..16524)
                     /gene="accB"
                     /locus_tag="KPG13_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG0986020.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
BASE COUNT         5972 a         2377 c         2376 g         5984 t
ORIGIN      
        1 ggtataggat taaaaagttt agtggtttaa agtttaacca ctaaactttt ttaatgtcta
       61 taatctacta ttatttaatt tactaaaaat aaaaaaactc attatattaa gataaaaata
      121 gaaaaataac tagaaaaata ctataatata tcagcttaat acagaaaaaa gttaaaaaat
      181 ttgagaaaaa taaaaatgaa aacgttttat attgacatag agaaaaagta gtgataccat
      241 ttaaatatag tttagtagct aaactatatt taaattataa taactatttt agggggatgt
      301 tatgaattta aaacaattaa gtgaaaaata cgaagaatat gtaattcaaa tgagaagaga
      361 atttcataag tatccagaat tatcttttaa agaaataaga acaacccaaa gaataaggga
      421 agaattacaa aaaatagggg tcccttttgt atctattgaa ccaaatatcg taatagctac
      481 tatacaaggc aaaaatgaag gtaagacaat tgcaattaga ggagatatag atgcgcttcc
      541 aatgcaagaa gattcagatg ttgaatacaa atcagaatat gatggaataa tgcattcttg
      601 tggacatgat gctcatggag ctatgttact aggacttgca catatgctaa atgatataag
      661 aaaagattta aaaggtagag tattattagt ttttcaagca gctgaagaag ttggtggtgg
      721 acacaaggag ataatacagt actttaagga aaatggagga ccagatagat taatagctac
      781 tcatgtttgg tccgatattg aagctggtaa aatttctgtt gaaccaggac ctcgtatggc
      841 tggtgggtct ggttggagaa tagacataac aggtagagga ggtcatggtt ctagaccaga
      901 tcaaagcata gatcctataa agccagcttg tgatatagtt ttaaaagtat cttctatacc
      961 agttaatcat ataagtgtac tagaacaatg tgttgtacat gcatgtgcaa tacatggagg
     1021 aactacaata ggaaatgtta ttccaaacaa tgcagaggta ataggtggat atagatattt
     1081 tactgaagaa tcaggcaaaa aagtgtttga cattataaag caaatgagta atggtgttgg
     1141 acaagtttat ggagtggatg tgaaggttac tcatactgga ggcataggac ctgttatcaa
     1201 tgatgaagaa gcagttaaga tggctaaaga aatagtttct aatatagatg gattggagtt
     1261 agattcttat gagccaatat gtgcatcaga ttgttatggt gaaatattaa aagagtattc
     1321 tggcttttat tgttatctag gggtaggtaa caaagaaaaa ggaataatat atgcacaaca
     1381 ccatcctaag tacaatatag atgaagatac attaaagtta ggttgcgaat ttatggctaa
     1441 atatgcagtt gaatttttaa ataaaaataa taactaaaat aaatcgaatt ttataaatgg
     1501 gggggatgaa aatgcaaggt gcattaaagg aaaataagaa aaaaagtggt ggaggatttt
     1561 taggatggat tgaaaaagtt gggaataaaa tgccacatcc tctagcatta tttacatata
     1621 ttacattgat agtagtagca ttatctgcaa tagcaagctt tttaggtttt tcagcaacaa
     1681 gtcctgctac aggaaagtta ataaaagtta tgaacttaat aagtgcaaaa ggtttagtac
     1741 tatttatgca agagtttgtt aagaacttcc aaaacttccc tgtgcttgga gtagttttag
     1801 ttatggcagt tgccacagga gtttgtgaaa agacaggatt tttttcaact gccataaaaa
     1861 tgagtttatc aaaagttaag gggaatgcgg ttgtatttat aatagctttt ataggagtat
     1921 ttgctaatca agctggtgat gctgcttttg tattagtacc aactcttgca ggagcaatat
     1981 tttatggatt aaatagaaat ccattagctg gtatattctg tggatttgca tcagttggtg
     2041 gtggatttgc aacagctgta attcctgggg gatgggacgt tacgctaacg ccaataactg
     2101 tatcagctgc tcaaactatt caacctgcat ttgatatgac attattagct agttattatg
     2161 cactatttgt atctgcgttt atagtagcta cagtatcaac aatagtaacg gttaaattta
     2221 tagaacctaa attaggtaag tatactggta aaccagaagg tatagatgat aatcaaggtt
     2281 ttgaggtcac aaaagaacaa gcaaaagcag caaagttagc aggttttaca gttttagctt
     2341 atgtagcgtt attaattgct ttgtgtatac cagctaatag ttttttaaga agtccagaag
     2401 gttctttaat atttggagca ccacttatgt cttgcttaca gttttttata gtaatactat
     2461 tcttcttgcc agggattgtg tatggaatga gggttggtaa aatcaagact gtaaaagact
     2521 taaatgatat attatgtcaa tcaatggcag caatggcacc gtttatcgta cttgcaatag
     2581 ttataggtca attcttaacg ctattctcag tgtctaattt agcacaggta ctagctataa
     2641 aaggtggagc attactcaac tcattgccac taccaccaca agctataata ctgctattct
     2701 tagtactagt agcgtttata aatatatttg ttataagcgg aagtacaaaa tggatgatat
     2761 ttggaccagt atttgttcca atgttaatgc aattaaacat acatccagca tttactcagt
     2821 ttgtatatag attaggagat tctgtaacca atcatttatc accattaaat gcattcttta
     2881 taatactttt agcaacagtc caaaaatacg ataaaaatgc aggtatggga actatattct
     2941 cagctatgat accttacact ataggttact ttgtagtact tgcagtttta gtagtagttt
     3001 ggatgacaat aggtatacca gtaggaatag gtggacaagt ttggttataa aacatattta
     3061 ataattttgg gaggaaagaa aatggaaaca actgaaataa gatacaattt tgatgaagta
     3121 atagatagaa gtggaacaaa ctgtcttaaa tatgatctta agagtcaatt tatgccagaa
     3181 tctccagaag atgcactacc actatgggta gcagatatgg attttgcatg tgcccctgag
     3241 ataattaatg caatgcatga aagaatagac agaaaaatat ttggatatag ctctcaacaa
     3301 actgatgatt attatgaagc tgtatgtgga tggtttaaga gaagatttga ttggaaaata
     3361 gaaaaagaga atatattctt tagcccaggg gttgtgcctg cgttaggatt tttcacacaa
     3421 atattaacac aaaagggtga tggtatcata atagaaaagc ctgtatacta tccttttgaa
     3481 gataaaataa ctaataatga aagaaaagta gttaatagtc ctttaaagtt tgaagatggt
     3541 cattattcta tggattatga tggatttgaa gaattagcta aagatgaaaa taataaggtg
     3601 tttatattat gtagtcctca taatccagtt ggtagagtat ggaaaaaaga agagcttaga
     3661 aaaatagtag acatatgtaa gaaatatgat ttgtggataa tatctgatga aatacattgt
     3721 gatattattc gtaaaggtga aaagcatata ccattagaag tagcatgtcc tgattataaa
     3781 gatagaataa taacttgtac agctccaagt aagagtttta acttagctgg tatgcaatta
     3841 tcaaatatag taatcaataa taaggaatta caaaaaaaat ggatgcttga aacagattct
     3901 aaattagcta tattcctacc taatccattc gcaatagttg ctactaaagt tgcttataat
     3961 gattgtgaag attggatgaa tcaagtaaat gagtatatag atggtaatat taaatatgta
     4021 gaagattttg ttaatgagta tatgcctaaa gtaaagataa taaaatctca aggaacttac
     4081 ttagtttggt tagactttag agggtatgga ttagatgcaa aacagcttga atatgtaatg
     4141 cttaacaagg cgaaggtatt gctagatgaa ggatacatat tcggagaaga aggagcagga
     4201 tttgaaagaa taaatgttgc ttcacctaga acaattatag aagagtgtat gaatagaata
     4261 aaatgtgcat ttgaaggatt ataatagtaa tatctaaata atatatttgt gtattttaaa
     4321 aaaagactat gctaaaaaaa tatttcaaag catagtcttt taaagtttta tgatgagttt
     4381 aaaaaatttc aaaacgttac tattcactta tatgtttata ttagaaataa ttccacaata
     4441 tactcatttt ctaaagttgc acttttatac accttgcttc accaatatca tgtgtaacca
     4501 aaatagtaga cttattctca ttcttaataa tactataaac atcatcacaa actaataatc
     4561 tagtctgata atcaagagct gaaaaaggtt catctaaaag tagaatatct ggatttacag
     4621 aaagtgttct aataagcgca actctttgtc tcatacctcc agataattct tttggataca
     4681 tacttctaaa atcccataat ccataagtct ttaaaaggct ttcaactcta tctaaagatt
     4741 ttttatcttt tttgccttga atttccaatc caatagttat attatccatt atatttctcc
     4801 attcaagcaa attatctttt tgaaacatat atccaatatt tccagtaatc tttatatcac
     4861 cactagtcgg ttttaaaaga ttagtaagaa tatttaatat tgtagattta ccacttccag
     4921 aaggaccaag caaagttaat atttctcctt ctttcaagtt aaaattgaca tcctttaata
     4981 cttcaagctc gccttcctta gtgtagaagt tcatatttat atttttaact tctactatgt
     5041 tgtcattcat tatgccaccc cctataactt ttagctcgta tatattatgt tattaacaat
     5101 atgacctatt tgtaccattt ttcataaata taatataaat attttgttta tttacgtatt
     5161 ttcttagtaa cttgtacgta taaattatag ataaataaat tataaggtag gggataaatt
     5221 tgagtaacat aataacgaat ttaatagagt caatatttac taatccaata actatattgt
     5281 ttttacttgc aataactgtg tattctgttt ttaaaataat agaaaataac agagtatatt
     5341 catttgtaag ttcaaaatta gacgaaatca atagagacta taagacaagt gagttttata
     5401 aaaaggtaag ggatgactac tatacatata gtaaagaaaa tccttatgca gacgtaaata
     5461 taacttcatt tatagaggaa gttgtatctg atttaaagca taataatttg ccattgttag
     5521 aaaaaattag aagtataaaa aattcatctt caataagtat attacttggg gttttaggta
     5581 cgtttgttgg tctttctaca atgcttttat gtgttgatac taaagacatt ataaattcac
     5641 ttccttcaac tattagtagt atgcaaactg catttacaac tagtatattt ggagttgtat
     5701 tttctctaat tataggttac tttactaaga taaaagattg tgaacatgtt ttgatacaaa
     5761 taatgttaaa atctgaaaat ttactaacat ctgaaataac tcattttaag tcagaaagaa
     5821 tggatttaaa agtagaagag gtaaaaaata caataaaaca aataagtaag tctatagaag
     5881 ctattgaaaa atttgataaa atatctaaag atttaaatga ctttaatgat gaatttatca
     5941 gtggtataga agctttaaaa agccttttag aaggttctca aagttctatt aagacatttg
     6001 accagagcgt aagaaaatta gataaacagt ttaatatttt aaatataaaa tttgtaaagc
     6061 tttttgataa atatgataat caagataata taaataaaga gattttattt gatataaaag
     6121 aatcttcaaa aaatatatac aatgccactg aaagccaatt caaaattaga gattatataa
     6181 aaaatataaa tgcaggattt gctctatatg aaagaagtgc tcaagattta ttaactaaat
     6241 taatgaccca tgaaaataaa atatctcaaa atcaaaaaat ccttttagat gaaaagttta
     6301 ccttagatga ctcaataaaa aatctctcaa gtattattga aaacttttct aatgacctac
     6361 aagttaaact ggatatgatg tttgaaaatt ctttagatat tcaagataag ttagatgtta
     6421 tgtttaataa ctcttttatg aatgatgaag ttcctcttga tagtgaagaa ttatttaagg
     6481 atgatgttaa taatgtattt aatcctttta gtgaagaaat ttatgagata gaggataaag
     6541 aaataaaggt cataggtgag gatgagttaa atgaataata aatatagaag aactgttaac
     6601 agagaatttg aaaagagtag cttttggcca acatttacag atttattatc tactgtattg
     6661 atggttgtga ttcttatctt atttagctca gaaagtattt caggttctgt tgaacaagat
     6721 ttagctaaga atgtcaatgc ttctgttgaa gaaactttta aaaaaagtgg aatacctgtg
     6781 aaggtagata aatctaatgg gcaagtaact tttggtgaaa aaaccatgtt tgatgtagat
     6841 agtgatgttt taaaaccaga ggctaaagaa atgctaaaaa tgtttgttcc aaagtacata
     6901 gagactattt ataaagatta tggtgactat atatccaaaa tagttataga aggtcataca
     6961 gatgatgtgg gaagttatat atacaactta gatttatcgc aaagaagagc atatagcgtt
     7021 gctaaattta tagtgggaga tgaaataggt gactataaat acaaagataa agtgactaag
     7081 catattattg caataggaag gtcaaaagct gaactcatca aaaatggtga caatagcgtg
     7141 aatagagatg catctagaag agtagaacta aaatacgaaa taaatattaa tcaaaataaa
     7201 taattaaatt tttataaaaa aaaagaaggc agttggtgtg agctttaagc cttctttaag
     7261 gaaaaagttt atttattttt gttgatactt aacatattat agtttttaga agcagtttgc
     7321 aatatattaa acgtaaatgg tcgtttaaaa tgcataactg gatactactg atgtaaaata
     7381 tttcatttta tataatataa aaattataca gtgtcaattt tctatattat tgtactataa
     7441 tctattattt aaaatattat gtaaaaaaat atcataaaaa cgcatatcat gttataatta
     7501 ctagagtatt atgaatatgg aggcgtagtt tgtgatagaa ataagtataa aaaataaaaa
     7561 aaattctaaa attaaaactt tgatactttt attgatagta tctatatcga tttatttaac
     7621 tggttgtact acatcagata atggaaataa tcaatcaaat aataatgatt ctttgtctag
     7681 tagtatatca agtgataaga atgtaaaaat tcattttatt gacactggaa atagtgatgc
     7741 aatattgatt caagatggta aaacttttac attgatagat gggggagata atgatgatga
     7801 gggattaatg gtagattacc taaacaatca aggtgtaaaa gatattaaat atcttatagc
     7861 tacccattct catgcagacc atttaggtgg acttgattct gttgtcaaaa actttaatat
     7921 agaaaatgtt tttgtatcga atggttcagc agaaacaaag agttataggg actttataaa
     7981 tgctttagca aataagggtt tatccccaag tgtaccgctt gaaaataata agttctattt
     8041 agaagattct tattttgaag ttctaaatac aaatggggga gatactacta atgaacaatc
     8101 tctagtcctt gtatatacta atgggaatga taaagtttta tttacaggtg atgctgaaga
     8161 aggtaccgaa aaagaaatac tacctaagct tgagaaagta gaccttttaa aagtagctca
     8221 tcatggttct cgtagtagtt catctcaaga gtttttagat aaagtaaatc ctgaatatgc
     8281 ggttctttta gtaggcaagg gtaattcata tggtcatcca catcaggaga caatgaataa
     8341 attaaaaaaa atgggagtaa aagtgcatag aagtgatgag tgttcagata taatatttga
     8401 atcaacaggt gatggagtat ttacaagttg taaagatgga agttataata aaggcgttag
     8461 agaagatgga aactctggaa aatcaaacag taatacaagt aaaaatgatt cttttaaaaa
     8521 taaacaaaat acatccaatt cagaagaaat agtgtatttt actccaaaag gtaagagcta
     8581 tcactctaca aaaaattgta gtgggctttc aagaagtaaa aaaatattat caggcacaat
     8641 tgcagaaagt aagaaaaatg acccatgtga tatatgttat ggaaaataac aaaaaaacaa
     8701 ccaatttatc tataaagctt ctgaacttta aatcatttaa aaaataaaat aaatgcataa
     8761 aaaaataagc tataatcaat agataacaca ttttatctat acgaatagct tattttttta
     8821 agtatttttt aatagctatt taataactct aaatacacca gttacaatac ctacaatttc
     8881 aacttctgag tcattgagca taattggttc cataaaatca ttttctggct gaagtcttat
     8941 taaatttcct tctttaaaaa acctcttaac cgtagcagat tcaccattta taagtgcaac
     9001 tactatctga gaatttaagg ctgtattttt tttgtctact acaacgtaat cttcatctaa
     9061 aattcctgca ttaatcatac tttcgccttt tactcttaat acaaaattgt tactgccctt
     9121 tactaaactg gcagggaatg gaatgtattc ctctatgttt tgttgagcta aaatgggttc
     9181 tccagctgtt atttgaccta ctaaaggaag ctctattatt tcttgattag ctccagaaac
     9241 atcatttact ttactacgtt ctaaaacttc aatagctctt ggtttagtag ggtcctttct
     9301 tatgtatccc agtttttcaa gtttatttag atgagagtgc acagttgaag tagatcttaa
     9361 ccctacagca gtacatattt ctcttacagc aggtggataa ccttttaata tgatttgaga
     9421 ctttataaat tccaatatca agacttgctt ttcagttaga tctaaataca taacatcact
     9481 catttccaaa catattttgt ttttatttcc atacacttta tatttttaat tataacataa
     9541 tccatttata tctgcaaaca tttgttcctg atttttaatc gtcctcaatc cattcaaaat
     9601 tcttgactat atcacgtttt acatttttct tatgagctgc ataaagctga gtagttgtga
     9661 cattatcatg gtcaagtaag ttttgaacat catatataga aaatcctgtt tggattagag
     9721 aagttgcagc cgttgctctt aatttatggg gactatatcc attgtctcta gtagtatcaa
     9781 gtggaataga tgtatatttt tttaccagtg ttcttatagc ctttgctgta attctcttat
     9841 tttgaagaga caaaaataaa gcatcttttt cttcatcatt taatagttca tctcttgttc
     9901 tttcgttttg caaataatct tttattacat gttcacaagt atgatttata ggcattaaaa
     9961 cttctttccc acgttttcta tatattttaa attcacctct tgaaaaatta aaagaggaga
    10021 tattgagttc tctaagctca tttaatctaa gcccgtatgt aacaaataat gctagtatag
    10081 ctttgtcacg taatttagtt tttctccaat ataccttttc tttctctgta agcccttcac
    10141 ctgtttctac tgattcaagc atcttagcaa cttcatctat ctcaaggcgc ttaatagcat
    10201 ctggctgagg tttaggaagt tttattggat tgaatccatc agttatatta ttatctatct
    10261 gagagtttct gtataagaat ttaaatagag tagagatgga tgattttttt cttgctaaag
    10321 ctctattgtt attttcaaaa atcaatgtgt ttttttctgt atttttgtaa tatctactgc
    10381 aataatcacc taagaataaa tttatatctc ttgattttat tgtattaaaa tcatcttctg
    10441 taatgtcttt tacacaatct gcatttgtta attctttggt ttcaatcaaa tatgaacaaa
    10501 aaaaattaat atcttctaaa taagcaagtc gtgtagaaac tgcaacagaa ccttttaaat
    10561 atataaaata atccttcata aatacaggaa gtttattttc caaaagtaga catttttcta
    10621 tgagttcatt ttctttgata acaatattat caggtttatc tgatttatta tgtactttta
    10681 taattttctt atcattattc aaaattaaac ctcctaaaac taaaatataa atcttttata
    10741 ttattttttg atatttatac taagtgagta taaatattta atttaatcat tttttacatt
    10801 tttaagagat tagcagtgct ttagatgaaa ttatcactaa ctattcccta aatccacatt
    10861 taaagactag ttgaatttaa gtactctttt ttatttctca atatatttat ataaatatat
    10921 tgagaaattt tttatgctct actagaatct tttttatata atatttttat atgtttctaa
    10981 cgaaaccaaa tattagtaaa atatgtattt atttaactaa agtttttaaa tgggctgact
    11041 aagcttaaaa tataggatat tatgagagtt actattgaaa aaataaaccc tattgccata
    11101 aagaaactta taaatatgtt atacttaact attaaactgg atatattcaa gttatcaaat
    11161 ccaaataagt atgtaattga acttattaca aaaataacta taactataaa gctcaggcca
    11221 tttaagctat tttttaaatc agcactacta agtgacatat gtgtagatat agaatacatt
    11281 aaaaacataa atataaaata cttcaatgaa tatacactag gtgttaacaa cattattctt
    11341 ataaaattgg aaatatcacc taaaataatg tgtataaaac catttatatt taaattatta
    11401 atcatgctta catataactt taaatctata ttttgagtca ctttcatata actatctggc
    11461 agtaatatct taaaaattaa aataatcatt attgttccac ctataatagg tgcaattcct
    11521 ataaagaaat ttcctatact ttgataaaga ttatcttttt tatagctatg acttacatat
    11581 cctagtatac catcattttt actttctatt ggtctgaaaa acttaactga atttatttta
    11641 tgcatgaaaa ttaaacacat tatcaaatga ctcaattcat gaacaaatgt tcctatagct
    11701 ccagttacaa taattccagt ttttccaaaa gatgaaaata taaatttatt atttagattt
    11761 tctgtaatgc taaaaatcaa tccaaataca ataaatattc ctatgaaaca aaaaatattt
    11821 attacactta tttttaaaat ttcgattata taaatcataa gttaaatcct catgtaaaag
    11881 tatttaacta ctctacttca tatccattac agcttaaaat tttacaagca ttttctatat
    11941 ccttttcttt tactaagata tagtctgtat cataagtaga aattgcaaat atgcttattt
    12001 tgttgtctgc taacaaacta cttaacttag ataaaattcc tacaagtgaa aagtctaaag
    12061 gacctagtat cttaagtatt ctccactcct tttctgactt aacatcagaa ggaatattat
    12121 tatttaaaca tacaacactt aattcatcat cagttctagt tatagagtaa aacttctctg
    12181 tatctatcca tgttggtatt ttactgtcat tatttaatct acatactgca tactcttcta
    12241 ccaataattt taatttcata atgtatattt cctccaatct tatttgttta cttaagatta
    12301 tacattaaaa gttacataaa ataaaacata taaagtaaaa aaggttcact atagtgtcct
    12361 atttctatag ccaaccttta aaacttaatt aaattctaat aaagagatat attatctagg
    12421 ttcaattata agtttaatgg ctgttttctt atcaccttcg atttctatat cagtaaatgc
    12481 gggaatgcaa actaaatcca ttccactagg tgcaacaaaa cctcttgcta ttgcaattga
    12541 ttttatcgct tgatttagag ctccagctcc tatagcttgt atttctgcaa cgcctcgttc
    12601 tcttagtact ccagccaagg ctccagctac agaattagga ttagattttg atgaaacttt
    12661 tagtacctcc atttcaacat ctccttgtta atttgttaaa gtatccatta cctaaagtac
    12721 taaatcataa ttgccattta ctaattacaa tcatcattta aagtatactt ttataaaaat
    12781 atacttctat aatcgtttta agaaaacctt tatttttttt aaccatttta ttatgaaaaa
    12841 actacaaatt tctttatatt ctataagcat tttccaatat ttctaaattt tttatatctt
    12901 tgagctataa tttcctcttt atccatatct ttgtaatgat ttaatctttc taaaatgtaa
    12961 gcttttattt cttttgaaac agcttctaca tctttttgtg ctcctccaag aggttctttt
    13021 ataatcttat ctattatttt taaatcatat aaatcattac ttgtaagctt cataacatca
    13081 caagcttctt tagctttaga tgaatcttta aatagaatac tagaaagccc ttctggagaa
    13141 atcacagaat aaactgaatg ttctaacata catacatcat ttcctactcc gatacctaaa
    13201 gctcctccac taccaccttc accaattaca aaagtgatta ctggaacaga aagtttactc
    13261 atttcaagta agtttcttgc aatagcttct ccttgtcctc tttcttctgc ttctagacca
    13321 caaaaagctc ctgatgtatc aataaacgtt attataggtc tattaaattt ttcagcttgt
    13381 ttcataagtc ttaatgcttt tctgtatcct tctggatgag gcattccaaa atttcttttt
    13441 atattttctt ttgtatctct tcctttttgg tgtccaacta tagtaacatt aaactttcct
    13501 atctgaccaa ttcctccaac catagaaggg tcatctttaa aaagcctatc tccatgaagt
    13561 tctaaaaaat tagaacaaat atgttctatg tattccaatg tcgtaggtct ctttatatct
    13621 ctacttaatg ttaccttttc atatgctgat agatgtgaaa aagcatcttc cttaagcttt
    13681 tccaatttct cattaagtga atttatttta tcacttaaat ctatattatt tgccttagaa
    13741 atagttatta attgtttaat atcattttct aagactttta ttttttcact attattttca
    13801 atcaaaacag ttcctccttt atgccctatt tcttgtgcat agcaagaatt tgatataata
    13861 cttccttcat ttttcttcta tctactatca tgtcaataaa acctttttca agtaaaaact
    13921 cagaagtttg aaatccttca ggaagttttt gatttattgt ttgctctatt actcttggac
    13981 ctgcaaaacc tatcaacgta tttggctcag aaattataat atcaccaagc atagcaaaac
    14041 tagcagttac tcctccagta gtagggtcag ttagaactga tatatatggc aatgattttt
    14101 cttcaagctt agaaagtgct tgagaagtct ttgccatctg cataagagat accatacctt
    14161 cttgcattct agctcctcct gaagcagaac aaataattat aggtaaatta ttttcagcag
    14221 catactctat tgaataagtt attttttccc caacaataga acccatactt cccatcataa
    14281 aatcaggatt cattacacaa ataactgttt taattccatt tattcttcca taacctgtga
    14341 caacagcttc tgactctttt gttttttcct tataagagtc tactttttct ttgtatttag
    14401 gaaaatttag tggattttga aattctacat cagaattaaa ctccataaaa gttcctttat
    14461 caatcaataa atttattctt tttcttgctc ttaagccata atgtcttgaa cattttggac
    14521 atacaaatga attttcttct atgtcctttc taaatacatg actatcacaa cctttgcaat
    14581 aggtccaaaa tttatcatct acactattct ttttaaagtt gtcatctaaa gaaatcgtaa
    14641 catacttaga ttcttttgat gataaaaatt tttttatcat aatctattta ctccttataa
    14701 tgtagcaaat tctttcttca taaagacatc ttctataaat ttggtatgat gagtatcttc
    14761 tctaaactcc tcacttgtta caagtgcctt ttgaaattct acatttgtat taataccttc
    14821 tacaataatt tcgtctagag ctctatatat tctacttata cattcttctc tagtttcagc
    14881 ccaacatatt aacttaccta tcatagaatc atataaaggt ggtatagtat atcctgcata
    14941 tacaaaagta tcaaatctaa caccaaaacc tccaggtaaa tttaatgatt ctatcttacc
    15001 tggtgatgga gcaaaattct ttgatgaatc ttctgcattt attctacatt caatagcatg
    15061 acctctaaaa gtaatatctt cttgtttaaa aggaatttta tccccattag ctatagatat
    15121 ttgaagtttt ataaggtcaa gatttgttac catctctgta actggatgtt caacttgtat
    15181 tcttgtattc atctctataa aataaaagtt tttatctttg tcaactataa actcaatagt
    15241 acctgcaccg atataaccta ctccttttac tatatctact gcaattttat gaagtttttg
    15301 tctttcttca tctgaaaggt atggacttaa agattcttct attacttttt gatttcgcct
    15361 ttgcatagaa caatctctat cacctaaatg tattgcattt gaatgtttat ctccaaatac
    15421 ttgtacttct atatgtctag gattttctat aaacttttcc atatataaat cactattaga
    15481 aaatgctgct agagcttctg aacttgccat atcaaaattt gaaaaaagtt cttcttcttt
    15541 tctaacgatt ctcatacctt ttccaccgcc tccacttgag gcttttatca taacaggata
    15601 tccaatttct cttgcaactt ctaaagcttc ttccttagtt ttcaaaacag attctgaacc
    15661 aggaacaaca gggatattca attccatcat agtttctctt gctctagatt tatctcccat
    15721 caaaatcatt tgttctcttg ttggacctat aaaaataata ttattatctt cacattgctt
    15781 tgcaaattca ggattctctg ataaaaatcc aaagcctgga tgaatagcat cacactttaa
    15841 tttaagagca agatagatta tattttctat attattataa ctcttactta tattatttgg
    15901 tcctatacaa atagattcat cagctatata tctatgaaaa caatctttat ctatttcaga
    15961 atagatagca acagttttta tacctagttc tttacatgtt ctgatgattc taactgctat
    16021 atcgcctcta tttgcaacta gtattttttt tatcaactta aatacctcct actacaatgg
    16081 ttttatctta aataaaggtt gattatattc tactagttct tcattactaa ctaatacttc
    16141 aattatttca cctttaattt cacttgttat ctcattcata agcttcattg cttctaatat
    16201 acataaagtg tcgccttctt caactacatc gccaacttta acaaagctat ctgcatctgg
    16261 acttggagaa tcatagaatg ttcccatcaa aggagctact actactgata gattctcatt
    16321 agatgtgaca ggtgtatcgt ttacaactgg cttttctaaa gaaagatcca ctactgattc
    16381 ttgttgtaca cttaaaacag gtgaacttgt agattgagct tttttagata cttctaatct
    16441 taaatcgcta ctttcaagtt ttacatattc taagttagtt gagtctatgg cctttaataa
    16501 ttccttaatt tcattgatgt tcattttgtt acccccttgt attattacct ggtgctaata
    16561 ttatatcaca aaaaacaaat ttataaaaga aaaagtgctg actagagaat caacacatca
    16621 atatatcttt taattttaaa ttaacaacca gtaacttata tttgttttaa ttttccatta
    16681 ttaatataaa ttataagtat aaaatgaat
//
LOCUS       DAELTA010000086        16639 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.086, whole genome shotgun sequence.
ACCESSION   DAELTA010000086 DAELTA010000000
VERSION     DAELTA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.086"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1445
                     /locus_tag="KPG13_002311"
     CDS             <1..1445
                     /locus_tag="KPG13_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986021.1"
                     /translation="GIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAIS
                     YLMSISYFIAENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAF
                     SELGFKLKSNSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSY
                     VNNEIDKLLYKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDF
                     KKLRQYQNNNYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQ
                     KSILEPEINSQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIV
                     SENMKFTYPFNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYD
                     DKERYIMLIKLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLV
                     PIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            1670..3283
                     /gene="ggt"
                     /locus_tag="KPG13_002312"
     CDS             1670..3283
                     /gene="ggt"
                     /locus_tag="KPG13_002312"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG0986022.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(3416..4267)
                     /locus_tag="KPG13_002313"
     CDS             complement(3416..4267)
                     /locus_tag="KPG13_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG0986023.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            4893..5786
                     /locus_tag="KPG13_002314"
     CDS             4893..5786
                     /locus_tag="KPG13_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG0986024.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            5786..7180
                     /gene="gltA"
                     /locus_tag="KPG13_002315"
     CDS             5786..7180
                     /gene="gltA"
                     /locus_tag="KPG13_002315"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG0986025.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            7546..9765
                     /locus_tag="KPG13_002316"
     CDS             7546..9765
                     /locus_tag="KPG13_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0986026.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            9912..10658
                     /locus_tag="KPG13_002317"
     CDS             9912..10658
                     /locus_tag="KPG13_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986027.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            10658..12538
                     /locus_tag="KPG13_002318"
     CDS             10658..12538
                     /locus_tag="KPG13_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986028.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            12657..14048
                     /locus_tag="KPG13_002319"
     CDS             12657..14048
                     /locus_tag="KPG13_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0986029.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            14155..15195
                     /locus_tag="KPG13_002320"
     CDS             14155..15195
                     /locus_tag="KPG13_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0986030.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            15684..16337
                     /locus_tag="KPG13_002321"
     CDS             15684..16337
                     /locus_tag="KPG13_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG0986031.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            16413..16622
                     /locus_tag="KPG13_002322"
     CDS             16413..16622
                     /locus_tag="KPG13_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986032.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         6607 a         1686 c         2830 g         5516 t
ORIGIN      
        1 ttggaataag aaatactata gttatcagaa ataagagaat tatagagtta tttaataaat
       61 atccagaaaa gtataagttt aaaaaattta taaagttaaa atgtgtactt ggtgttttat
      121 taatagctat ttcttattta atgtcaatta gttattttat tgctgaaaat attatgcttt
      181 cagtttttat attagttaca ataatacctg gaactttttt atttttttca tcaacgatgt
      241 atatgattat tgatgctgtt aagaagagaa aaagttttta ttataaggga cagaatttaa
      301 ttgcattttc tgaacttgga tttaaattaa agagcaatag tagtgtattg gctgtaatag
      361 caatattaat agctacaagt gtaactatgc tagggtttac catatcactt tattacgata
      421 tagataggaa cataagtgaa aattataaat acagttatac cataaattta ggaaattctt
      481 atgtaaataa cgaaattgac aaattattat ataaatataa aaaagataat aagataatct
      541 ttgataaaac aatagaactt atagacaaag atataaatat tgaaatgtat tataaaaatg
      601 gaaatagtta tatagaagat agaacgtctg tagatattat tagagaaagt gattttaaaa
      661 aattaagaca atatcagaat aacaattatg aagaattaac ttcaaaggaa tctgtgtact
      721 atgtttctga ttcatacaaa aaaatatttt ttaaagactt tgaggttgaa aaaataaact
      781 tgcatattaa agatacaaat gagtatgata taaatctgtt taaagtacaa aaaagcatct
      841 tggagccaga aataaattca caagctacgt ttgatttaat tgtagcaaaa gatgacgtat
      901 ttaatcagtt aaaattacat ggaaattcta gattaataag attaattgat ataaaaaatg
      961 aaaaaaaaga actggatttg actttaaaat taaaaaatat agtaagtgaa aatatgaaat
     1021 ttacttatcc ttttaatttt acatcaagta ttgaaagtta taataacttg ataaaattaa
     1081 gtggtctaat gctgtttata ggcatatttt tgtctgtagt atttctttta tgtacaggta
     1141 gtataatctt atttaaacaa ttatcaaata tatatgatga taaggaaaga tatattatgt
     1201 taataaaact tggagcaaat aataaagata ttgagaaaat aatatcaaaa cagttaaaag
     1261 ttatattttt aatgccatta gttgtaggta cagttcataa tttatttgct atgtctatag
     1321 cacaaaagtt tataccaagg tcattacttg taccaattat cataactcta gtcatttatt
     1381 ttataggata ttttatatat tattttttaa ctttaaaata tgctctaaac atgataaaag
     1441 aataaattat ttttaagtca aaagaattag atatatgttt tgaataaaat aacttattta
     1501 agtgcagata gaaaatacta gaattgagtt taagatattt atttaaagac aaaattaaag
     1561 ttaggaaaaa gtctatattt tatatcattt tgtatcagtt acattacttg atgttatata
     1621 ataaatctaa gcagaatatc aaataaaaaa tgacgaggag ctgaaattta tgaagtttaa
     1681 tgcctaccaa cataaatact catcaataag aaatgtcgtg tatgctaaaa atggagcagt
     1741 tgcaacatca acaccattag catctcaagc tggacttgaa atactaaaaa agggtggaaa
     1801 tgctgttgat gcagcagttg caacagccgc cacattagca gtagtagaac ctacaagcaa
     1861 tgggattgga ggagacgcat atgcccttgt ttggatagag gaagaaaaac gattgtatgg
     1921 tttaaattct agtggctttg ctccagaaaa tatggaatta aaaaattata ataatatgaa
     1981 agaaatgcca aaatatgggt ttggagcagt aactgtacct ggaataccag cagcttggtc
     2041 agaattaaat aaaaaatatg gtaaacttag cttaatggaa tgcttatctc cagcgataaa
     2101 ctatgcaaga gagggatacg tagtatctcc taatgtagct aaggtatgga aaaaatcata
     2161 tgaactctat gaaaaagaac ttattggaga agagttcaaa ccatggtttg atacattttc
     2221 taaagatggt aaagctccag aggctggaga tatatttata tgtgaagaac aggcaagtac
     2281 attagaagag atagcaaata ctcaggctga aagcttttat agaggaagac ttgctgataa
     2341 aatagatgaa tattctaaaa aatttaatgg ggctataaga aagagtgact tagagtgttt
     2401 ttatccaaca tgggtagaac ctataagtac agaatataag ggatataaaa tatttgaaat
     2461 cccaccaaat ggtcatggaa taacagtttt aatggcactt aacatattaa aagaattgga
     2521 attagaagga aatatagaaa atgtagaaga catacataaa attatagaga gtttaaaatt
     2581 agcttttgca gattctaaaa catatgtaac tgatatagag catatgaagg taaagattca
     2641 ggaattacta agccaagagt atgctaaaaa aagaagttta ttgattgaca ataaagaggc
     2701 tttgtatcca actgcaggag agccatattg tggtggtaca gtataccttt gtactgcaga
     2761 taaggatgga aatatggttt catatataca gagcaattac ataaattttg gttcaggaat
     2821 tgtgatacca agaactggga ttgctttaca tagtagagga aataatttta atttagaccc
     2881 taaacaccac aatgtagtaa aaccatttaa aaaaccttat catactataa tacctggatt
     2941 tttaggtaaa gaagataaag caataggtcc atttggtgta atgggagctt ttatgcaacc
     3001 acaaggacat attcaagtgt taacaaatat gattgatttt ggattaaatc cacaagaggc
     3061 gctagatgct ccaagatggc agtggataaa aggaaaagaa atagaagttg aaccagagat
     3121 gccaaagcat ataatagatt cattaattga aaagggtcat gaaataaaag taattcatga
     3181 tactgtcgac atgggacgag gtcaaataat atttaaaaca gaacaggaaa gttatatttg
     3241 tgggacggaa tcaagatgtg atggtcatat tgcagtttat taatattatc aactaaaata
     3301 tatataataa atttaaagag tatatgaaat aataagtaaa ggcgaaagtc tacaatgact
     3361 gacgccttta caaaatttaa gctatcatat gctgatttta tatagattat gagccttatt
     3421 cttgaccagt tttgatttca ttgtcatcat aactaatcca gtcactaaag ctaccaatat
     3481 aaatcttgtg aggtatatcc agttctctaa gagcaagact gttaacacaa gcagatatcc
     3541 ctgaaccaca agataaaatt acttcatcat aattatttaa atctttaaag aattttttta
     3601 aaaattctat atctttaagt gagccatttt caaagtcact ttttattaaa tccttacaaa
     3661 aataattttt tgcacttggt atatgaccag cttttgagta agcaggttca actaaaccct
     3721 ggtatctctc attagcacgg caatctatta ttacagtatc ttttttgtat aatttagatt
     3781 taacatattc cattggaaca actaaatcat tgtttatatt tggtctaatt tccttacctg
     3841 tacaacttgg tatattcact ttctcttcca attctccacc ttcctttacg aaagatgtta
     3901 ttcccccatc tagtacatag acatttttaa gccccaaatg ttttaattgg aaaaataatc
     3961 tgcaagctcc atttaaatct ccatcatcat aagttacaat cgtagtgtca ttatcaacac
     4021 ccattttttc taatttttct tttagtatta aagggtcttg taaaggattt ttaccaccat
     4081 gttcttttgt aggagatgat aaatccttgt ctatatctaa gataaaactg cctttgatgt
     4141 gtcctttttt ataagaatca ataccataag ttctattgat aagatcaaat ctacaatcta
     4201 ttacaactag attatcattt ttttctagtt ttgatataag ttcttttgct gaaataatgt
     4261 ttttcaaaga atgtacctcc tgtgtttata ttatctttat tttaatttat attaattatt
     4321 aactttataa actaatatta ttataattta ttacataagt aaataaatct ttagaaattg
     4381 taaaaatttt atataaaaat tcaaataatt aatgaatatt gaaaatttta gatatataat
     4441 aaaataagag tatattttat acaatatgaa agctggtatt gcaatagatt aaagccttat
     4501 acacgtttag tttttaaaga aattagtgtt attatgaaaa cctaagttaa tttattaaat
     4561 aaataattga ataaaaaaat tttattatgt atttataaaa tttttgttac aaatttggca
     4621 taatatagta tactttaagt gtgtattagg ttaaatctat aacaaattag acgtatgata
     4681 tagacttagt gtttattggc atttacaaga aatttgttaa tatcaactat ttgtagtttg
     4741 ggcagttata attattatag gggggatgtc caatacaaaa caatcaacca agaactaata
     4801 ttatattata aagagttata gttttgtgtg tttatgtttt taattaaatt taaagtggga
     4861 aaaatatatt tgttagaatg gagaatagcg agatgagtaa taaaatagtt agtaagagac
     4921 aattaacaga tagtatttat ttaatggaaa tagaggcacc tagagttgca aaatcttctc
     4981 aaccaggtca atttataata attaaaaatg atgaaaaagg agagaggatt cctcttacaa
     5041 tagctgacta tgatagagaa aaaggaacag taactattgt attccaaaca gttggtgctt
     5101 caactaaaaa gttagcaatg tttgaagaaa acgattttgt aatggatttt gtagggccat
     5161 taggacaagc aagtgaattt atacatgaag atatagaaga attaagaaat aaaaagatat
     5221 tatttgtagc tggtggagtt ggttctgctc cagtttatcc acaagttaaa tggtttaaag
     5281 agcatggatt agatgtagat gtaataattg gtgcaagaac taaagaatta attatattag
     5341 aagatgatat gaaaaaagta gctaaaaatg tatatgtatc aacagatgat ggtacatatg
     5401 gatttaatgg aagagttaca gacctattaa aagatttagt tgataatcaa ggtaaaaaat
     5461 acgaccaagc aatagtaatt ggaccaatga taatgatgaa atttatgtgc caattaacta
     5521 aagaactaaa tatacctact atagtaagtt taaatacaat aatgatagat ggaacaggta
     5581 tgtgtggtgg ttgtagagtt agtgttggaa atgaaactaa gtttgcatgt gtagatggtc
     5641 ctgaatttga tggtcattta gttgattttg atcaagcaat gagaagacag tctatgtaca
     5701 agactcaaga gggaagagct atgctaaaac ttgaagaagg cgatagtcat catcatagta
     5761 attgtggatg tggaggtaac aaataatgga tgctaaaaaa gtaaaagtac cagttagaga
     5821 acaagaacct gctgtgagag caacaaattt tgatgaagta tgcttagggt ataataaaga
     5881 agaagctatg gcagaagcta atagatgttt agcttgtaaa aaaccaaaat gtgtgggtgg
     5941 atgtccagta ggaatcgaca taccagggtt tataacaaaa attaaagaag atgatataga
     6001 aggtgctgct aaagtaatag ctaagagcag ttcattacca gctgtttgtg gtagagtatg
     6061 cccacaagaa agtcaatgtg aaggtgtatg tatacttgga ataaaatcag atgctgtatc
     6121 aataggtaaa ttagaaagat ttgtagcaga ttggtcaaaa gaaaatgaca taaatttatc
     6181 tgatacagag cctaagaaaa atcaaaaagt agcagtaata ggaagtggtc cagcgggtct
     6241 agcttgtgct ggtgatttag ctaaaaaagg ctatgatgta acaatctttg aagcaatgca
     6301 tgagccaggt ggagttttaa cttacggaat acctgagttt agacttccaa aacaagctgt
     6361 tgttcaacct gaaatagaca atataagaaa attaggtgta aaaatagaaa ctaatgtaat
     6421 tgttggtaaa acaattacag tagatgaact tatagaagat gaaggatttg aagctatatt
     6481 cataggttca ggagctggtc ttccaatgtt tatgaatata ccaggggaaa atgcaaatgg
     6541 agtattttct gcaaatgagt ttttaactag agtaaactta atgaaggctt atagagatga
     6601 ttatgataca cctattagtt ctggtaaaaa agttgctgta gtaggtggtg gaaatgttgc
     6661 tatggacgct gctagaactg cattaagatt aggttcagaa tcatatatag tttatagaag
     6721 aagtgaaaaa gagcttccag caagagctga agaagttcat catgcaaaag aagaaggcat
     6781 tatatttaat acattaacaa atcctaagga aattttagta gatgaaaatg gatatgttaa
     6841 aggtatggtt tgtataagaa tggaattagg agagccagat gattctggaa gaagaagacc
     6901 tatagaaata gaaggttctg aatttgtact tgatgtagac acagttataa tgtcacttgg
     6961 aacaagccca aatccactaa tatcttctac aacaaagagt cttgatataa ataagaagag
     7021 atgcttaata acagatgaaa atggtcagac ttctaaagag ggtgtatttg caggtggaga
     7081 tgctgttact ggagcagcaa cagtaatatc tgcaatgggt gctggtaaaa cagcagcagc
     7141 atcaatagat gaatatttaa aagcaaaagt taatgcttaa tttgtaaatt aaagagaggg
     7201 tgtctcaaaa tgaacttttt agttcatgag gcactctttt tgtacaaaat caaatatgtt
     7261 ttaatcattt caacaatgaa aatttcattc ctgtagaaag tctaactttt aggtcttagt
     7321 acaatctcta ttttgatgta gccacttttt tagatggttt aataatttat cagttattat
     7381 tgtaatattt gatactaagt aacataactt tgtctaacta tgataaaata gttatacaat
     7441 aaaggtcttg taaggagtga ataatgtgtg gctaataaaa tcagatttgt caaatctaac
     7501 attaaataat agaaaaataa attgtgtaat taatggtatt ttaatatgaa aaaaagagaa
     7561 aaagtatttg atgggctagt ttttaagcat atattaatct tattaatact attagttttg
     7621 attgatttaa caagtaccat catatatgca agtaactcta tggaaattac ttctagaaat
     7681 atgattgagg catcaaaaag agaacttgaa aactatttag aagtaaacat atctttatta
     7741 aaagctctta gtcaagatga tagattttct aatggtgaaa catcactaat tgaaaaggga
     7801 aaattattaa gtccatatca aaaggaatat aatcttttta tgattggtat aacagataca
     7861 aaaggaaatg cttcaagtac atatagggaa aatgttaact caataaaaga taagcctagt
     7921 tttgagaaag caattaaaac aaagcaagta gtagtttcag atattgaagt cagtaatgta
     7981 acaggtgaca aagtatttat catatatgta ccaattgtaa aaaataatga gatgattggg
     8041 accatttttg cttcttttta ttttcaagat gtaaataatt taataagtag aagcaatttt
     8101 gatgattcta ttaaatttct aatgatagat aaagattata ctattatttc tcatccaaac
     8161 aaaaaatatg taaatgataa aagtaagatg cttgatttag aagggaatat tataggaacc
     8221 actaaaagtg aaatcttaaa aaatataaat aaaaaatgtc aaggtgattt tttatcttgg
     8281 gataattgga gattatataa tgtaaaatat acgaacataa agtggactaa ttggacttta
     8341 gtatcaaaat gtaatatatt taagaacttt aaaaatctaa tagttaattt tatgataaag
     8401 ttgtattttt atatagtaat atttatgata ttgtggaaat taagtaatgc taaattaata
     8461 gaacaactta aaaaattggc ttattatgat tctttaagtg gaataaaaaa taaagaaaag
     8521 tttagaaaag attctatgta tattttaaag aattattatc aagataattt ttacttagta
     8581 caattggatg tcaataaatt taaatatata aatgagatgt ttggatatgc tgaagggaat
     8641 aagatattaa ttcatatatc acaggtttta aataataata caaataaata tgaaatttgt
     8701 gctaggatgg ataatgacca ttttatattg ttgattgctt gtaacactga agatgaactt
     8761 ttaaatagat tatctaaaat aaataaagaa atttgtaact taaatactac taactcatct
     8821 aagtataaaa ttgttatgtc aagtggaata tataagatta ctaaaaagga tgatattaaa
     8881 aagattgact tgttaattga tagagcaaat attgcagcaa aaagtaaaaa ggaaaaatat
     8941 gaacattcat attcattttt taatgaagat acaagaaatc gattgtacaa ggaaaaacga
     9001 ttggaagata atatgaataa agctttagaa aaaggtgaat ttatagtata ttatcaacct
     9061 aagtatagtt tagatgatgt aaatgaaatt gaaggagcag aagccttgat aagatggaat
     9121 agtccagaat ttgggtttat ttcacctata gattttgtac ctttatttga aaagaatggt
     9181 tttattgtaa atattgatat gttcgtgttt gaagaagtat gcaaaacatt aaacaagtgg
     9241 ataaataaag ggtatactcc tgtaccaatc tctgtaaata tgtctagagt tcatttatat
     9301 agggataatt ttatagaaaa tataactgat ttgataagta aatacaatat atctcctgag
     9361 tttattgaac ttgaactaac agaaagtgtt gtgtttgata atttaaatat attgattgat
     9421 ataatgaaaa agattaaaga gataggattc cttatatcaa tggatgattt tggttcggga
     9481 tattcatctt taaatctgtt aaaagacctt tcttttgata ttttaaaatt agatagagga
     9541 tttttaatcg aaacaacaga tacaaaacgt ggaaaaatta ttatttctaa gattgtggaa
     9601 atggcaaaag cgattgatat taaagtaatc tgtgagggcg tagaaactta tgaacaagta
     9661 gagttcttga aagagatagg ttgtgataag gttcaaggat acttatttgc taaacctatg
     9721 gtattagatg aatttgaaaa acacttaaac tttaaatttg attaatcatt aataattatt
     9781 agactataat ataccctaac aacttactta caaatatgta agttaattgt tagggtattt
     9841 ttatggttaa agtagtatat tcacgatatt atggtattgt agtaaaaata aaaaattatg
     9901 gagaatataa aatgaacata ttgagtatta gaaatatatc aaaaacatat tgcggaaata
     9961 ttccatttaa agcattagat aaggttagtc taaatataga aaagggagaa tttgtgtctg
    10021 ttatgggacc atctggaagt ggtaaatcta ctcttttaaa tataatatct acagttgata
    10081 gacagagtga aggtgaggtt gtattagatg gatatgatgt aagtaaattg aaaggagaaa
    10141 aacttgctga atttagaagg aaacaacttg gttttgtatt tcaggatttt aatttgattg
    10201 acactttaac tgttggcgaa aatattatgt tgccacttac actagagggg gcaagtatca
    10261 aagatatgaa tatacaaact aaaagtatat ctaaattttt aggaatagat aaaattttag
    10321 atagaaaaac ctatgaaatt tcaggaggtc aagctcaaag gtgtgctatt gctagagcta
    10381 ttataaataa acctgccata cttcttgcag atgagccaac tggaaatctt gattcaaaat
    10441 ctactgatga tgttttgaaa ttatttacta ggataaacaa agaacaaaag gtaacaacac
    10501 ttatggtaac tcatgaagca tatagtgcaa gttattcaga tagagtaatt tttataaaag
    10561 atggttgtat atatacagaa ataaaaaaat ctgaaagcag taattctttc tattctgata
    10621 tactagctgt tttgtctcaa ataggaggtg taagatagtg gattttagaa agtttgctta
    10681 taataatata gttaaaaatt tcagggcata tttagcatat tttttaagtt ctgttttttc
    10741 tataatgata ttttttgttt ttgcagtgtc tatgttccat ccaattataa caaaatctgg
    10801 aatgcagagt ggttctactg catttatggc tcttatgact tcagaattaa taatattcgg
    10861 ttttagtcta ctttttatat tgtactcttt aggaaatttc ttgaaatata gattgaaaga
    10921 ttttggagta ttgatgataa ttggaatgag taacagacaa ctgaaaaaat tgatattaat
    10981 tgaaaatttg ataataggat ttttagcagt gatgttagga atattacttg gtttatcaat
    11041 ttcaaaattg tttttattgt atttaagcaa actgttttat atgaatttaa ctgaatttta
    11101 ttttccagta aaagcaatgc tattgactat tgtatcattt atgattttat ttttgataac
    11161 aggaccaatg agcttaaaat tattgagtaa aaagagcatt ttagagcttt tagtaggtac
    11221 aaaaaagcct aaaaaagaga taaaatcttc aaagctattt ggaataattg gtgtaatatg
    11281 tcttatatta ggatatgcta tgatactatt tggcaataag ttaaaaataa atggaggtat
    11341 gtatacaaca gcactattaa ttactttggg gatattttta tttttttctc aatttactat
    11401 tttgatatta aagtatttaa aaaataaaga aaaattttat aagaacaaag taaatatgtt
    11461 acttataagt aatttgatat ataaattaaa ggataataca cgattgttat ttttaataac
    11521 aatactttta tcggctactt tagtagcatt tacaacaact tcaacacttg taagttctca
    11581 aggagaagct tctaaaaata attttccaat ggtttactca tatttttcag agcataataa
    11641 tgaaaaagaa tatgaagaac tacaacaaat tagagaagca atcaaaggat atgattataa
    11701 ggagttaagt tttcctgtac ttaaatataa tagggaagtc ttactaagtg taaagaatta
    11761 taacaaatta tcaaaggaat tgggattaga taaaattagt tcaaagttag aaaaatatga
    11821 aggggttata attcctagta gcaattcaca agaacatata aattctttaa aacaattaaa
    11881 aaattatagt ataaaaaatt taaatataga agtaaaaaaa tcaaaaaata aaataatttt
    11941 tcctacagga ctgtttagta aagttgtagt tataagtgat gacttataca atgaagtaaa
    12001 aggtgaattt tcaaaaatta attttcatgg atttgattat aaaaactggc aaagcagtgc
    12061 acagataact gaaaatttaa aagaaaaaca ttttaatttg gatagagaat ataacaggtc
    12121 atatttttta acattaccag atatgtattt agtagagtta caacaaagta aaattcttca
    12181 atttatgggt gtttttatag gagtgatatt atttatagca gtttttagtt ttttatactt
    12241 cagattatat acagacgaga tagaagataa tgtaaagtac aaaaaactat caaaaatagg
    12301 tctatctttt aaaaatatga ataagattgt tagtatagag ataggtacat tgttttttat
    12361 accgtatttt atagcaatta taaatagcat aatttcttta ctgtttttaa atcaagtatt
    12421 aaataatagt tttaagttag aaaatttggg tatattacta ctgatatcat ctatatattc
    12481 actttatttt tacttattaa aaaaaattta tactagaaaa gtttggtttt ttgattaaag
    12541 tggtatataa tagatatata aattaatatt taaaaagtag ttaatattag gggagggcat
    12601 ttgcacctcc cttgtaatat gtggaaaatt gaatattaga agaaggagga gacttgatgg
    12661 aaaatcaaca attattagga acagaacgaa ttagtaaatt actactaaaa tattctatac
    12721 cagctataat aggtatgttg gtaaatagtt tatataatgt agtagataga atttttattg
    12781 gaaatatacc aggggttgga ccacttgcaa taacaggtct tggtgtaaca atgccaataa
    12841 tgacaataat acttgcattt gggatgttga taggtatagg aactactact accatatcaa
    12901 taaaacttgg gcaaggtaaa gtagaagagg caagaaaact tataggaaat gcaatgacgt
    12961 tatctgtaat aacaggaatt atcataatga tacttggaat attatttgca aataaaatac
    13021 ttacattgtt tggagcaagt gaaaatactt taatatatgc aaaatcatat atcaatatta
    13081 tattattagg tacagtagta aatcttcttt ccttttcttt aaaccactca ataagagctg
    13141 atggtagtcc taaaatttca gcaggaataa tgatagtagg atgtttaact aatatagttt
    13201 tagactggat attaatattt ggattcaatt tgggaataca aggagctgca attgctacag
    13261 taacttctca ggctcttaca gctattttaa ctatagggta ttatataagt ggaaaatcaa
    13321 acttacgatt cagtaaatct aatctaaaat tagataaaaa gctaataaaa gcagtgtttg
    13381 caataggtat gtcgccattt gctatgcaac ttgcagcaag tttagttcaa gtaatatcaa
    13441 atattgcact taaaactcat ggtggagatt tagctatagg ggctatggct actatttctt
    13501 ctattgcaat ggtatttttg atgcctatct ttggaataaa tcaaggagcg caacctatta
    13561 taggtttcaa ttatggagct gaaaaatatg atagggttaa aaaagcatat ttaggttcat
    13621 tagtagtagc tactataatt ttatgtatgg gtatggtagt agttatgctt ttcccagaag
    13681 ctattatagg tatatttaat aaagaccctg aacttatgaa tatatctgtt aatggattaa
    13741 gaatatactt acttatgttg ccaattgtag gcttatcagt tacaggaaca aactttatac
    13801 aatctatagg aaaagcaaaa atggctatgt tattgagcct tttaagacaa gtaattttat
    13861 tgataccagc agtattaatt cttcctacat tcttaggttt acagggtgta tggacagctc
    13921 aaccagtgtc tgattttata gctacagtaa ttacaggtat agttgtattt agagaattaa
    13981 aaagatatac tcctaaaact gaaaaattaa atgaaaatga aagactaaac gagataacta
    14041 ctgagtaaaa atataaattc agataattga gcaagaaatt gcaaaaaata cgattatatg
    14101 ataaaataaa accgctgtca aggataagag aaattctaat tatggagtga ttttatggaa
    14161 tttacaaaga aaagcaaatt aaaaaatatg tatgtaaaca tgaatgtagc tagaatgttg
    14221 tctaagataa atgagtataa gggtagacaa ctgctttaca aaaaacaacc aaaagaaatt
    14281 ttagaaaact tagaaaaaaa atctttagta gattgttctg aatctacata cattggaaat
    14341 caaagagata gtagtaattt taatctagaa aagttgataa gtaatgaagt tacacctaga
    14401 agtagagagg aattgagtat tgttgaatac agagatgtgg taaaaactat taacagtgct
    14461 tatgagagta ttcccatcag ttcacaaacg attcttgagc ttcatggata cttatataaa
    14521 ttttcatcta ctagaggagg aagttataag tcagataatg attttattga acacaattta
    14581 aaacctagcg aatttataag tattgattca agtgtcaata aagttgaaaa agctgtagaa
    14641 gaaatatgtg aagcttataa tacattgata gaggaagatg aaattgatat attaatatta
    14701 atttctgcat ttgtgttaga tttcatattg atacatccat tcaaagaagg taatataaaa
    14761 atggcgagag tacttatcct attgttacta aataaaaatg ggtatgaagt tggaagatat
    14821 ataagtttag gaaagatatt tgatgatagt tcatatgaat attatagtaa tttaaattat
    14881 ttgaaagcct caataggtag tgaaaaagca gatatgaatg catggattga gtatttttta
    14941 gagacgatat taactgcata tgagaagctg gatgatagtt taaatatatc tgataagaaa
    15001 agacaaacaa aaactagtag aatagagaaa ataataaatt ctacccttgg atattttaca
    15061 aaagaagata taagagattt atgtcctgac attccagaac ctactataaa tagagttttt
    15121 aataatctaa gaaaacaaga taagatagaa gttgttgcaa gaggaagaag tgcaaaatgg
    15181 aagaaaaaat attaaaaaat gtttagataa ctaaaatggt atagtgtttt acactatacc
    15241 atttttaatg tcattagcat gagcaataat ttaacaactt aagcaatatc aattttattt
    15301 attgattatt tttgaactga aaaatggcgt tatatggtat acctcagtgg taattaattt
    15361 tggatgagaa gatataatat tacagaatat attaggagta tatgtagaaa aaatataata
    15421 aaagtgtgag gcttagtaat taacaaatta tgtggcttca cactttttta taaaataaag
    15481 atacttagca gaagaatatt ttagttgata tgtttataag atggtacttg tttgggtaaa
    15541 agtataaata gtaaaaaagt agaagtaatg tgtataaaaa cgtaagttac attctgtaat
    15601 aatagttatt ttataattca acaaaagaat agaatgggta tatttgtgaa ataataatat
    15661 aaaataatct taggagtgag cttatgaaga aaaaattatt atatataaat gtaaattcaa
    15721 aacctgaaga cttatcttca agtaaaactg tagcaagaaa atttataaat aaatttatgg
    15781 aaaaaaataa agattttgag gttgaagaga tagaccttta taaagaacat ataccaagac
    15841 ttgagtatca gtactttgaa aaaagaaata gcatagttag tgaagaaaat gctaaaaatt
    15901 tagacgataa ggatagaaaa gaacttacaa agattagaaa tctttgtgac caatttgtga
    15961 gtgcaagtgt atatgtaata gcagcaccta tgtggagttt atcttttcca gcaccactta
    16021 aagagtatat agattgtata attcaagatg gaaaaactat atcttttgaa ggcaatgata
    16081 aaccacaagg aattttaaat gatattgata gaagtatggt atatatacaa tcttctggtg
    16141 gacacatacc atgggtgttg aagccagtta tgaataaagg gttaaattat gtggaaagta
    16201 ttatgaaatt tataggaatt aaaaaatttg atgaattgtt agtagatgga actggaacta
    16261 gtgaagaaga aagacaagct gctatagaaa aagcagctga aaaaatagat ggtataatag
    16321 atgggatgaa attttaatta taatatttta ttaaaaatat aaaggaaaaa cctcttttaa
    16381 tgtagaaact tatattgaag gaggcgagaa tcatgcgaga agaaaaaagt aatgaaaagt
    16441 atgattgtta ttggtgtaat caagagaata atttctgtgt agaaataaaa gataatatag
    16501 tcatgataga tgatggcact ggtacgttaa aacaagccgt tttcataggg tataaacaaa
    16561 tccaaattaa tttaaactgt tcacattgtc aaaacttgaa tagaataaaa ttaaatttgt
    16621 agaattttaa aatggaaat
//
LOCUS       DAELTA010000087        16300 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.087, whole genome shotgun sequence.
ACCESSION   DAELTA010000087 DAELTA010000000
VERSION     DAELTA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.087"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(260..670)
                     /locus_tag="KPG13_002323"
     CDS             complement(260..670)
                     /locus_tag="KPG13_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986033.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(886..2268)
                     /locus_tag="KPG13_002324"
     CDS             complement(886..2268)
                     /locus_tag="KPG13_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0986034.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2279..2638)
                     /locus_tag="KPG13_002325"
     CDS             complement(2279..2638)
                     /locus_tag="KPG13_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0986035.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(2843..3622)
                     /locus_tag="KPG13_002326"
     CDS             complement(2843..3622)
                     /locus_tag="KPG13_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG0986036.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4083..4694)
                     /locus_tag="KPG13_002327"
     CDS             complement(4083..4694)
                     /locus_tag="KPG13_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0986037.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(4786..5775)
                     /locus_tag="KPG13_002328"
     CDS             complement(4786..5775)
                     /locus_tag="KPG13_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG0986038.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(5842..6705)
                     /locus_tag="KPG13_002329"
     CDS             complement(5842..6705)
                     /locus_tag="KPG13_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG0986039.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(6811..7407)
                     /locus_tag="KPG13_002330"
     CDS             complement(6811..7407)
                     /locus_tag="KPG13_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG0986040.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(7447..8250)
                     /locus_tag="KPG13_002331"
     CDS             complement(7447..8250)
                     /locus_tag="KPG13_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG0986041.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8286..8726)
                     /locus_tag="KPG13_002332"
     CDS             complement(8286..8726)
                     /locus_tag="KPG13_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG0986042.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(8739..10841)
                     /locus_tag="KPG13_002333"
     CDS             complement(8739..10841)
                     /locus_tag="KPG13_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG0986043.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11102..12805)
                     /locus_tag="KPG13_002334"
     CDS             complement(11102..12805)
                     /locus_tag="KPG13_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG0986044.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(12831..13790)
                     /locus_tag="KPG13_002335"
     CDS             complement(12831..13790)
                     /locus_tag="KPG13_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0986045.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(13926..14444)
                     /locus_tag="KPG13_002336"
     CDS             complement(13926..14444)
                     /locus_tag="KPG13_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG0986046.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(14487..14972)
                     /locus_tag="KPG13_002337"
     CDS             complement(14487..14972)
                     /locus_tag="KPG13_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG0986047.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(14969..15586)
                     /locus_tag="KPG13_002338"
     CDS             complement(14969..15586)
                     /locus_tag="KPG13_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG0986048.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(15651..16013)
                     /locus_tag="KPG13_002339"
     CDS             complement(15651..16013)
                     /locus_tag="KPG13_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0986049.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
BASE COUNT         5232 a         2974 c         1591 g         6503 t
ORIGIN      
        1 tttttaactt agactttatt tttttattac ttatttgagt cttatttatt ttattctcac
       61 tccatatgtt caattacatt tgtctaaatt ctttgatact tttattacat aattttaaca
      121 agcttaagtg ttatttgggt agtgcagttg ataattattt aaataatcat caactgcatg
      181 tttatatcat tacagtagct taatgtattt aatcatactt acttaggatt ttgaaatatc
      241 ttagagatta tatttaacat tagtcaattg ctgcataatt gtaagcaatt gaaatttgag
      301 acccatcttc attttctata gtgataggct tcataatttt taatccttca gtttttatct
      361 tttctaattc ttgttccatt ttcttaattg tttcctcaag gtctttttga cttacatctt
      421 tgactgcatt agggtcttta gcatagtctt ttaattcttg aatatgttta gcatactctt
      481 cataagtata atgctcaaca ccatactttt ccaattcagt agggtcagtt attcctgatt
      541 ttattagaga ttcaaattta ggtaaagcaa tattagatga atcatttcca tttgcaaata
      601 ctgctccagt tcctcctagt accattgcag ataccatcat tgcacttata agggattttt
      661 ttattttcat aaccaatctt cctttcttag agatttattt gggtagtgta gtttaaaatg
      721 actattgcaa tagttatcaa ctacactctt agactatcac agtagtcatg tttatgtcaa
      781 gcctttttaa aaaacttttc aataaaaaat actatctcaa agcaaaatta attattttga
      841 gatagtattt taattctaaa tcttttatat tactaagatt tatggttatt ctatccatgt
      901 tctatattgt tctttattaa ctgctaacaa attccctttt cttaatatga caaagcgatt
      961 atcattatag ccacttcgct caattgaaac ttctcctgaa tcagtataaa gtacatcctt
     1021 actattacta gatttattta catacgtttc ttcactattc tcagaattag ttactttgct
     1081 catagatgat tcttcatgct ctctaaattc aattacagat acttttgccg tactaaaatg
     1141 agtctctcca tcacttttag ctttactaac aagaatatca gattctactt tgggagaata
     1201 taaatcagtt gatttactac ttattgaaaa tccaatttta tcactttgtt cattttttgt
     1261 taaaccaaaa gattcaacct cattaatctc tttattttct gttgcaaaaa caagacctgt
     1321 cccacaaact aatacagttg aaactaaaaa agtaacataa gttgattttt taaatttcat
     1381 aatattaact attctctttt ctatttttgt ctttccaaat ccactacata ttgtcattga
     1441 aaatggatta ttctcctctg ctagacttat tagtgtggtt gcataatcag ccctatattc
     1501 ttcaccaagt atactcataa ccttttcatc acaactaaat tctatgtctt tatttagtaa
     1561 aaaaaacatc acccaaacta agggattaaa ccaatggaca acaagagcta caagcataaa
     1621 taactttaac agattatcaa agctattaat atgaaccatt tcatgagtta aaacaaagtc
     1681 taaagtttga ttattagcat aattcatttg ctttggcaaa ataattcgtg gttttaaaat
     1741 accagatgta agtggtgtcc tagtaaattc agatatgcaa acaataaatc ttctaaaacg
     1801 gctattctta cacatccatt ttttaatgaa ctcatgattt tttatatgaa ttgaagtttt
     1861 aaaaagatta attattttaa aatatctaaa gcataaaagt atgctaaaca taatagtaac
     1921 tataaaagac aaaatgccca acatttccaa catcataata ttttcaatat tagacattat
     1981 ttcaaattcg tcagaattgt ttttacctgt agagtcacta gctttcttaa gagtataatt
     2041 ttctgttgat gtattaccca attccacatc aaaagtcgat tcaattatac tataaatact
     2101 aaaaccagaa tttaaactta ctggtattag aagtttaaat aaaacaatca accacaataa
     2161 tacaaatacc tttttaggta attttttaaa taaacatgct cttataaaaa taactattaa
     2221 aagtaatact ccagaagtaa aactcatttc taacaattta atgaacataa atcttacctc
     2281 actttaattt ttcaacaatt tgtttaagtt ttgtaatctc atcacttgaa agattttctt
     2341 tattaataaa agctgaaaga aatttttctg aagaccctcc aaacatttta ttaattaaag
     2401 tttctgtttc ctgtttttgt acttcctctt tgcttattaa agctttacaa acaaaattcg
     2461 gctcaattct ctctattgca ccttttgaca ctaatttttt tataacggta tacgtagtgt
     2521 ttctattcca tcctgtttct tcttttaaaa tttttactaa ctctccagcc ttgctttctc
     2581 ctctctccca tagaacttcc attactttaa gttctgaatc aaataatttt atctccatat
     2641 aaaatcgctc ctcaaatata gttatcacaa tagtcattac tataataaaa aactatcata
     2701 gtagtctata ctatgtcaat catttatttt ttaataataa aaactgtctt tttaaaaaat
     2761 tcttataaaa gacagtttta tcaatgttat actttaatgt ttaattaaag ccatatttta
     2821 tttaaatata taactatcta tcttagtaca tagtataaga tttttcttta tctaatagta
     2881 tatcctctac atctgggttt tcttttacta attgtctata aaaattacaa ttagtaacac
     2941 ccacatatgg aattagccat agtagaccta ttccaagagt agctattgaa agtatatacc
     3001 atcctataaa gctaaagttt agtacaaata atttaagtac atttcctttc atcatatcta
     3061 aactagcacc cattgattta aatacaccca tattatcctt gtcttcacaa attaagaaag
     3121 tagcttgaga aacatataat agcaatatat aaactggtaa cataagaaca agtattataa
     3181 gaactagagt tattccagta gaaataatag tccctattcc aacattatca cttgataaat
     3241 attcaattgc tccactagta agtaaaactc caaatgataa aaacgctcct ataattgtga
     3301 ttataaaaga ataaatatac acaataattg attcaaataa ataaaaacca aaacttctta
     3361 aataaattct cccacttaca aagatatctc ctatcttgac tttttcatct ttagtaattt
     3421 tcaagaacat acatgtaaat cctgctgaaa cgcaaaaagc tattacagcc actacaatag
     3481 tagtcaatgg ttcaaaaggt aatcctacaa ttgatattaa tactatcaat gcaatatata
     3541 ctgccataga aattgcacat atcccccagt ttccagataa ctgagcttta gatactcgtt
     3601 ttaattcttg ccttgatacc ataaaaaaca cccctttcat tttataaaaa aacttttaaa
     3661 aatataaata atcttttaac gtataatatt aatatatcat attttcgact atataagata
     3721 caattattca ccatgtttta tcaagatatg ccataattgt attactagtt ttatatagtc
     3781 aacattacca aatacatcaa ataaatttga ttaattattc aacaagtaat aaaataattt
     3841 ctattaatta tactttattt agtaaacaaa ttatttaata aaaaaattac ctaatacagt
     3901 aaattaattg gtaagataaa ttatttatta ttagtaaagt aaattattta ctatttatca
     3961 agataaatta tttataaaat aaatcattta taaaatataa tttatacttt gaaaataaaa
     4021 taaactatct tccaaacatt ttattatatg cttaaaagat agttttgtgt ctttaaaata
     4081 aattaaattg tatatattcc ttcacataac ttttgtatat tactaggtgg ttgccattta
     4141 ccatcttttt tagtatatgt aatcatagtt gtaacttcct tagttggttt cttattgtaa
     4201 aggtctataa tcatttttgt catatcatct tgaacttctt tttctttttt atcgtaattc
     4261 ttttcatcag ttacataaga ttttactaaa gtgttcgctt cactcattac tttactagta
     4321 tctaaagctt tacttacaac tttcaaagtt atcttatcct tactagaaga ttccactgtt
     4381 actgtctttt caacaccttg aattaattta acaattgttt cgtatatctt ttcagcattt
     4441 tctttaccat aaagaggtgt caattgagca atcattgaaa ccttgtcctt gctgttataa
     4501 gtttgaatct gtttatattc agcttctgtt aatgcatcag aaattccttt ttcatcatac
     4561 ttatacattt ctaatgcagc taaattagct tcaaaattcg actttacttt ttcttctgca
     4621 ttagaacacc ctatagttag aactaaaact agagccataa tcgtagataa tattaacttt
     4681 tttaccattt tcatacaaac atcctccctc aattaaaata ataaaaaatt tatttatcaa
     4741 cattataaca taaatttgtc taactataga attattttga atattttacc acttccagtc
     4801 tattccaata ccgttttctt ttgctttatc gtatattctt tttgctgtta cgacatcttg
     4861 tacacctatc ccaacagttt taaatacaat tatctcatca tcatcttctc ttccaggaat
     4921 agttccgttt ataacatttc ctaaatcacc actaaaatca tctttactta tcttaccttc
     4981 ttttaatgga attaatatat ctcctgactc agataaaact gcatctgttg attcaaaaaa
     5041 tatcttagac gctctagtta aacagtttgg gcacaactct tgcatatctg gcatatatga
     5101 acctactgca cttattaaag ctcctttttt cactaagttt ccattaagta ctggttgttt
     5161 tgatggagta gctaaaacta tcacatctgc gttatctatg gcttcgtcag atgattcaac
     5221 tgcaactatt ttagtattat atttacttaa ttttaagttc atttcttctg caaatttttc
     5281 tctactttcc ttgtttctac taaaaactct tacctcatcc aaatttctag cttctatcat
     5341 ggcttcaagt tgtggctcac cttgtcctcc tagacctatc aaagctccaa tcttagaatc
     5401 ttttttagcc aatacgtcta tagctgctcc agaggctgct ccagtacgaa tctgagttac
     5461 ataagtacca tccaatactg cactaacttc tccagtttct ccatctaata atagtactgt
     5521 tgctggaatt actggtttgc ctttttgagc atttttagga aatacagata caatttttat
     5581 tcccccacag cctaattctt ctacgtatcc tggcataaat aacatactag catcttgact
     5641 tggaacagat atattggttc tcaaaggatt tacactcttt ccttcacaat aaattctaaa
     5701 agcttcctta tcagcttcaa ttgcatctct cattgtaaat acttttttta tgtcgtcttt
     5761 ttttaaaagt agcattttta tacccccaat tttaataagt ctctatgcta aatattatag
     5821 cataacattt aatttaatct tctatttcaa atcaaataaa tctacatact ctggttttag
     5881 catagaactt atcaacgctt tatttattga aaattctggt attcctgctg catatggagt
     5941 tatatcatac aaatcaaaat atacaactag attctcatct tgcaagtaaa agttttgatt
     6001 ctcttttata cctttaaaat tatatattcc gatattttct tcatcctttt tttcaagttc
     6061 agctatttgt cttttaattt cttcatctat aacttcttta tatttagtat tatccttaaa
     6121 caagtccatt aattctaaaa attttccagt tctcatatcc acattatatg ctatatcttg
     6181 ataaaatcca tgtgctccac cactataagt ataatatctc acttttatac taagagcaga
     6241 atcagtattc tttttaagtt caaagtctac atttgcaaca aacttatttt tctcgtcagg
     6301 attatcctta agatactgct ttgcttcttt atatgattta ttataaaatt caaatatgtc
     6361 attttttatc ttatcatttg tagacctttc aacaatttta tttgaatttg ttattacagg
     6421 catgtttata gtactttcta aatactcatc atttttcact attgttttac ttcctattgt
     6481 acttactgat aaagtttgtt tactatttgt attacccttt ggtaatgact ggttactcat
     6541 aacagtcact gcttccttat tactatttaa attaattgaa gcatctttcc ttaaattatt
     6601 atatttatag tcatgtttaa ttgccaaaac agccattata gtcaatgtta aaacaaatac
     6661 atatcttaaa actttagctt ttgtatcgac tttaaaaaat ttcatattat cattttcctc
     6721 ctaaatataa atctctctta ttatatttgg agtttctagg gttttttatc catataattt
     6781 aataaaagat taatttttat ttataaaaaa ttaatctttt tgttttaaaa tattaatatg
     6841 tcttatcaaa cagtttgaaa tttcaccaag aggtaattgt aacataacag aacctctatc
     6901 aaaagctacc attggcattc catatactat acaagaattt tcatcctgtc caattgtaaa
     6961 tgaaccattt tctttcattt ttaaaagtcc acttgctcca tcataaccca tacctgtaag
     7021 tattattcct attgaatttt tacctgctac ttctgctact gaatcaaaca aaacgtctac
     7081 tgatggacag tgaccactta ttctttctcc tttttcacaa gttacataat atccttgttt
     7141 atccgtacac aatttcatct ggtaaccccc aggtgcaatt aacacatttc cttgttctac
     7201 tctatcatta ttcttagctt ctcttacatt cataatacac tgcttgtcaa gccgttgtgc
     7261 atacatataa gtaaatatag ctggcatatg ttgaactaca acaatccctg gtgttgattt
     7321 tggtaggtct tttataattt caagtattgc ctctgtacca cctacagatg cgcctatagc
     7381 aataaccata ttattaaaac gttccacatt accgcctcca aaaaatcata tatcaaaaat
     7441 acctttttat cgtttatata tagcaggttt tacatatttg tatttagtac tacttctgtt
     7501 gattacttct gcatgtccta caaacaaaaa actatctttt ttggtagcat tataaaattt
     7561 atttataagt tcttctcttg tttttaaatc aaaataaatc ataacatttc tacaaaaaat
     7621 caaatcaaaa tgttgatatg agaaatcatt cattaaatta aacggcttaa aaattacact
     7681 ttttctaatc ttatcacaaa ttttaaatgt accatccttg ttgtctataa aatacttctt
     7741 cttccataac tcaggaacat tgttaacatt tgattctatg tatatacctc ttttagcttt
     7801 ttcaagtgag ttagttgaaa tatctgtggc tatgatttta atgttccaca actctttatt
     7861 atatccaaaa taatcatcca aagtcatagc tattgaatac gcttcttctc cactagaaca
     7921 tcctgcactc catatattta tgtttcttat tttattattt tcttcccaaa aaggaagtat
     7981 actattctgt ataaactcaa aatgttgagg ttctctcata aaaaatgtgt ggttagtact
     8041 caatctattt attaggttta tagcttcatt tcctgtccta tctttaaata atagattcat
     8101 atattgacta aaagaactaa gttttttctc tatcattgta ttgtataagc gaccttcaat
     8161 taacgctctt tttttactta aatcaatacc atattctttt tttacatggt ttacaagaac
     8221 tataaattct tcatctgtga gttttatcat gcaaaaactt cctcctgcta atttatcaga
     8281 actaactata tgctattaat caaaaacttt cgaatatcta aaattaatat agaattatct
     8341 tcaactttcg caatctcttt tatatcttta ttaatattag tgttgttaga attagtcgtc
     8401 tctagtaaat ttagattttc tatatgaata acatcaacaa ctttatctac aataattcca
     8461 acttgatact catcaacctt tgtaattatt atacaagttc tctcattata atcagcttta
     8521 ggaagattgt actttatatg catattaata actggtatta ttcttcctct caaattaata
     8581 actccactta tatatgttgg aagacatggt acatacgtta ttgattgaaa tcttatggtt
     8641 tcaatcacat tttctaattc tattccataa ataacatcat tgacataaaa tgttaatact
     8701 tgtttaataa tttcatcttt agccatactt taaatcccct aatatatttc tataatgcta
     8761 tctgcatcta taattaaact tatacttcca tccccaagta ttgtacatcc cgataaaccc
     8821 ttatttttta tattaaatcg gttaaaataa actggaaatg gtttcacaac aacttgctga
     8881 ttacctatta tttcatcaac aaaaatgcat actgctttat gctcattttc aataattatc
     8941 atattaccat tcatcagatt ggttactttt gtttgaatat tatatatttt atgtaacctt
     9001 ataatgggaa tacatgctcc atatattgta attaactctg tcttatttcc ttctgtaatt
     9061 atttgattta agtctgtaag tttaaatgtt cttcttatgg atgatattgg taaagtaaat
     9121 agtgaatctc caactttaaa cttcataccc tctactatag aaagagttaa tggtattttt
     9181 atagtaaagg ttgtcccctc atcctgttta ctttttactt caattgaacc tccaactttt
     9241 tcaatacttt tacgaacaat gtccatgcct actcctcttc cagaaaattc agttatagat
     9301 tcttttgtag aaaagcctgg agtcataatc aaagaattga cctcgtcatc agtatactca
     9361 ttactttgat gtttaagaag accttgttcc ttagcttttg ccaaaatctt atctctcata
     9421 atgcctctac catcatctga aatacttatc acagcttctc cgcctagatt ttcagcagat
     9481 aaaactatct ttcccacttc ttctttacct ttcgctatac gctcctcaat aggctctatt
     9541 gcatggtcca tagcatttct tataaggtgc ataaaaggtt ccgctaaatt atcaattaca
     9601 ctcttatcca cctctgtttc tgctcctata gttattaact ctacattttt atctaatttt
     9661 ttattcatat ctcgtacaat tctatgcatt ttttgaaata caccagcaat ttgaatcata
     9721 cgcatagaca ttactatatc ttgcaattca tctgtaagct tgtgtaattg tcttgttgcc
     9781 ttattgaaat tatctatatc aacacctgta gaatttacgc tatttacaac cattgattcc
     9841 ataattacaa tttctccaac tatattttga agtaagtcca atttttctaa atttacactc
     9901 ataatactct gcttacttat tggtaaatgt tcattttctt cacttagact atctttttcc
     9961 atatcagttt tttcatttaa aacatcttct tgtatctcat cttctgtata gttattttga
    10021 taacttttac ttactttatt attatctttt aatttatttt ctactgcatc attgtttgat
    10081 gtacttttca tttctatttc ttttttacta atatttatgt attcttttac atagagatta
    10141 tcttctatag ttttataaac tattgcctta tcctctttag ctacaaattg tatataaaac
    10201 ccataacttt taatatactc tgtagactct gcatttgttt ctacatcttt tggataaaaa
    10261 tctataaatt cacagtaatc tctaagttca aatacaagca tgaaagctct cacattttcc
    10321 atcatgcaac cttgttcaaa gattattttt actacatcag agtcttcttt gtattcaaaa
    10381 tttgaatcga ttacttcttt ttcgtcttta ctttctaaat ttgcattatc ctttgatttc
    10441 atagaacttg ataattccaa agtactcaca tactcttcaa ttccttcaat aaaattagag
    10501 taatcagaag atatacaacc tcttgattgt aactcatcta tatctcttct tataaaatct
    10561 aatgattgaa agactaaatc aaataatagg tcattgtcta aaacttctat tttaccttca
    10621 cgtatagcat aaaacaagtc ttctacacta tgtgctaaag cagatatatt gtctaacccc
    10681 atcatagatg ctgaaccttt tatcgtatgc attattctaa aagtttcatt aatgttgtta
    10741 tgagtaaata ctttctcttt ttctatttga atcataattt catctaattg ttcgagtaat
    10801 aaacttgtct caaatataaa catatctaac atttgtctca tactcggttc aatataattc
    10861 acatttatca cctacgacct acaaataatt ttctattata tatgcatctc ataatttaaa
    10921 tccttgatga ttctcttatc tcactatatg tacctcataa tttaaataca caattattct
    10981 cttacttcaa tatatataca tctcataatt taaatccttg ataattctct tatttcaata
    11041 tatatacatc tcataattta aatacatgat aattctctta cttcaatatt cacctcataa
    11101 tttaaaccca tgatgattct cttacttcac tttcctttag atgaaatttt tctactaatt
    11161 cttttaaaat ttgagcctga gcagacatct cttctgaggc tgctgcactt tcctctgctg
    11221 ttgctgagtt catttggact actatggaaa tttgctctat tccagataaa atctgtgtaa
    11281 ttgctacttc ttgactatgt acagccgaag aaataccatt aatataatta gtagtttttt
    11341 ctacaccatt tactaccaag tccaatgcat ttgcagtctc tttagcaatt ctgacaccat
    11401 tttctactgt tttaatagct tcttctatga gaatagctgt attttgagct gcttttgcac
    11461 ttttaccagc caaagaccta acttcatctg ctacaactgc aaatcctcta ccatattgac
    11521 ctgctcttgc tgcttcaaca gctgcattta atgcaagaat attagtttgg aatgctatct
    11581 catctattgt tttgattatt tttgatattt cactcgattt ttcacttatt tcacccattg
    11641 atgtcatcat atcatccata catctacttc caaaaccaac ttgtgatgca gattggtcag
    11701 atacctcaca tgcaagacta gcactatctg cacacttctt aattccatct gatatctcat
    11761 taatcgtgga agctaattct tctactgaac tagcttgttc agtagcaccc tgtgaaagag
    11821 cttgtgcacc tgctgcaact tgttctgaag cacttgcaac ttgctctgct gataaattta
    11881 tttcagaaaa atcaccatta aaagagttta ttatactttc taaagatatt ttaattgatt
    11941 taaaatcacc tataaattct tggtctatct ctactcttaa atctcctttt gcaatactag
    12001 ttaatgtact gtctataagg tctatgtaat ttaaaagcat actttgcatc tttttgacag
    12061 aactcactaa tagccctatt tcagatttac tttcttcaac gtctatactt gttcttaagt
    12121 ccccagatgc aattcttgaa aagtgttttt ctactttatt aataggaatc actattttta
    12181 attttattac tgtatattct attattgtca atattattaa aagggaaagt ataaatattg
    12241 ttcttaatat tagactttgt aattcttttt ttacttctac aattttggaa tttgaacggg
    12301 taactatatc ctcttgaaat tcatttatta aagaatccat tttctcttgg gagtttatat
    12361 agtcactacc aaaaattata ctttgagctt tactcttatc attactttcg actgccttta
    12421 ttgcactttg ttctgttggt actaaagaat ctgatatttc ttttgcttct aagattttat
    12481 ttgactcgct ctttgtaaga ccaatttttt ctaatttgtt taaagctgat tctcttgttt
    12541 ttccgtataa ttctttttca taaatatctt tattcttttt ttctcctgta aatacatatc
    12601 ttcttaatac atcacttatc tctctagttg tatcttttat atactgactt tgagtcctca
    12661 aattattcat atcattaata cttttttcta ctttttcaaa tgctttataa gatgaaatag
    12721 aagaaaaaat ccctgctaga acagccaata ctataataat cagcaataca attgctaatt
    12781 tagaaagctt tacttctctt ttcatatctg tactccttca taaagtaagt ttatatcttt
    12841 tttaatcctt tcattgtact cactacgcta caatcttttc ctaagttttt accctcatac
    12901 attgctttat ctgctaagga tatgttttct tctaatgata tatttgagtt tacttctact
    12961 actcctaatg taatagtgca ctttatttta tgttctttat agttgaaatc aaactccttt
    13021 atctctttac ggattctttc tgaaagtatc ttagcacctt ctattggagt atttgtgagc
    13081 attatcaaaa actcttctcc accccatctt gatacagtat caatttgtct acaattcttt
    13141 tttattatac ttgatattac tgttaacacg aaatcccctg cttcatgccc ataagtatca
    13201 ttgataacct taaagtcatc aatatcacca aggataaggg atattgatgt ttcactatat
    13261 ttattaattc ttctttgctc cataagctga ttaattatat gtcgcctatt gtaaagacct
    13321 gtaagataat ctcttacaac gagtctattc aatttttcaa gagaagattc cagttcatta
    13381 tttatctttt ttaatttatc ttgagatttc tttacttcaa gatgagctat aacacgagct
    13441 tttaattctg tcatactaaa aggtttcacc atatagtcaa ttgctcctac accaaatcct
    13501 tttactatgt ctgtatctgt attttttgaa gttataaaaa ttataggtat atctgctgta
    13561 cgagcattag attttacttc cttacaaaac tcaaaaccag agttatctgg taaaattata
    13621 tcaagcaaaa taatgtctgg tataaattct tcaataaatt cttttgcctc tattgcatta
    13681 aatgcttttt taaccttaaa attatctttt gtaagtgttt tatctattaa gtcacacatc
    13741 agaacactat catcaacgac taaaattgtt ccctgttgct tatttttcat atacaaacct
    13801 ctcttaaaca taatatattc aaattttaat gatttaaaat ttttatactt aataattata
    13861 ccactatatt cttaaatgtt ctcatcacaa tctttttata ataacactcc aactacatcc
    13921 ctatgttaca tgatagtgca atcaaaccca ttgattaatt catctattaa aacttcatct
    13981 ttatctgtca aaagtttctc caaatctagt aaagtaatta ttttaccttc taattctcca
    14041 actccactta caaaataatc cgaatattct atagtaactc ttggtggagg tgatatttta
    14101 cttttttcta agcttactat atcatcaact ctatcaacca caaatccaac taccaagtcc
    14161 tttatttcag ttactactat atacatgcaa tttgtagtca ttaattcatc ttttttaagt
    14221 ctcaaacgca tatctattat cggaatgatt tttcctctta gatttattat gcctttagaa
    14281 tatgtaggta gttctggaat aaatataatt tcttgatttc cagtaatttc tattaccttt
    14341 tcaagttcaa ccccaaaatc ttgctcatct actttaaact ttagatactt aattccttca
    14401 tcaattagtc cataatctaa atatgggtca tctggaaatc tcatttaata cctccctcct
    14461 aaaatacact ttctatgaaa tatatattat aatttcttct tacctttatt tattgaatct
    14521 acaagtaatt cacccgtctc agtgtaaaaa cgtattgttc taccataatt catacctgtg
    14581 tcctcagcta atattggaat atttaatcct aaaagagttt tttttactgc ctcaacattc
    14641 ctatgtccta tacttgcaat tactgaattg ctacttccta aaaacatctg agcacctcca
    14701 gcaatttttg cttttatata tattctattt gcacctatat tctccataga tttaatgagc
    14761 tcatttattc ctgtgtcagc aaatttacac ttattttcaa tgttaatagc atctttacta
    14821 tatggcaaca taacgtgtaa cattccacca attccagcta ctttatctat caaacatact
    14881 cctacacaag aaccaagtgc ataagtaact aaaacatttg gtctataggc tatcttcata
    14941 tctgcaatgt tcactactat ctctctattc atacttctaa tcccaacctc acgagtattt
    15001 ctctaagtga gctcatttct ggaatcatca aaatgtgact agtaaaataa ttatcactca
    15061 ttttaaattt attttctata aataagactt tgtcaccaac atctgaaaat cttatcatag
    15121 gtacatttag aacagctcca accatatcaa tacatatatc tggaattgaa acacttatag
    15181 tcatgtttaa catagatgca atagcattta catatgaact tgccataata tttcctattt
    15241 ccttaattgc tgaaatttca attgcctcaa tctcatctaa aaaagcttct tctttattaa
    15301 aaagagaact tagcataagt tgagttattg attcatccaa caaaaacata aacattccat
    15361 tgatatcacc tttcatatcg agtaaaatac atactacttt gttttctgga ccaccaagca
    15421 aatttgtcgc ttcattatat tcaagtatct tgaccatagg tattaatact tctacattgc
    15481 tatttatcat ggatgcaagt gcagtaattg cattcccaga gcctatatta ccaatttctc
    15541 taagagcatc tatatgaaca tcatcaagtt ctaaataatt tataatataa acccctccct
    15601 ttattattgc atttatttgc ttttcattgt attagtgtat atttacactt ttattttaaa
    15661 gcattatcta caactttaac taatgtttct gttttgaagg gcttcactat aaaatctaac
    15721 gctccaagtt ttatggcttc tacaatcata ttctcttgtc ctattgcaga gcacattaca
    15781 accttagcac ttgaatccaa cttttttatt tctcttagca cctctaaacc atcttttttt
    15841 ggcattgtaa tatcaagcaa catcaaatca ggcttttcat atttaaatat attacacgct
    15901 tcttctccat catttgcttc tattaaatta ttatagccac ttttactcag agcttcttta
    15961 attatcattc tcataaaaat agcatcatct actatcaaaa ttttattttc cataaaacta
    16021 actcctccat atgtacaaca ataaacgctt ttatcttcag ttattgtcta tactatctga
    16081 ctatttttcg ctacacaata aatacatttc attgataaat aaaagtaatt atttatgcca
    16141 tattatactt aattctctta acataatttg tcaacaaaaa aacttatata tctattgtaa
    16201 tatatgtttg gcgttttgac gagttattaa aaatataatt gtataaaaca taaaaagtta
    16261 tcaaaagtaa ttaaattata ataaaacaac tttatacctt
//
LOCUS       DAELTA010000088        16243 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.088, whole genome shotgun sequence.
ACCESSION   DAELTA010000088 DAELTA010000000
VERSION     DAELTA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.088"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1318
                     /locus_tag="KPG13_002340"
     CDS             <1..1318
                     /locus_tag="KPG13_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0986050.1"
                     /translation="KLNFKIKLQRIAYTDNLTGANTIDKFVIDANKILCKNTQVKYAL
                     LYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEMFARISADNFIIIMKYIEE
                     EDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKMAK
                     GGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSEAL
                     VRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIYLRKWIDAGINPVTVSVNV
                     SRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMNNLKKIGFLI
                     SMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVIEMAKELGMKVI
                     SEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLVRG"
     gene            1494..1862
                     /locus_tag="KPG13_002341"
     CDS             1494..1862
                     /locus_tag="KPG13_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986051.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            1864..2589
                     /locus_tag="KPG13_002342"
     CDS             1864..2589
                     /locus_tag="KPG13_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986052.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2583..3347
                     /locus_tag="KPG13_002343"
     CDS             2583..3347
                     /locus_tag="KPG13_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986053.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3569..4819
                     /locus_tag="KPG13_002344"
     CDS             3569..4819
                     /locus_tag="KPG13_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0986054.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5010..5894
                     /locus_tag="KPG13_002345"
     CDS             5010..5894
                     /locus_tag="KPG13_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0986055.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6256..6873
                     /locus_tag="KPG13_002346"
     CDS             6256..6873
                     /locus_tag="KPG13_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG0986056.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            6981..9512
                     /locus_tag="KPG13_002347"
     CDS             6981..9512
                     /locus_tag="KPG13_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0986057.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            9946..10647
                     /locus_tag="KPG13_002348"
     CDS             9946..10647
                     /locus_tag="KPG13_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0986058.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10697..11779
                     /locus_tag="KPG13_002349"
     CDS             10697..11779
                     /locus_tag="KPG13_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986059.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            12040..13140
                     /gene="vanG"
                     /locus_tag="KPG13_002350"
     CDS             12040..13140
                     /gene="vanG"
                     /locus_tag="KPG13_002350"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0986060.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13137..13943
                     /locus_tag="KPG13_002351"
     CDS             13137..13943
                     /locus_tag="KPG13_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0986061.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            13961..16099
                     /gene="vanT"
                     /locus_tag="KPG13_002352"
     CDS             13961..16099
                     /gene="vanT"
                     /locus_tag="KPG13_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0986062.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         6186 a         1621 c         2901 g         5535 t
ORIGIN      
        1 taaattaaat tttaaaatta aattgcaaag gatagcatat acagataatc ttacaggagc
       61 aaatactata gataaatttg taattgatgc taacaaaata ctatgtaaaa atactcaagt
      121 aaagtatgct ttattatata tagatattga taaatttaaa tatataaatg atttatttgg
      181 atatgaagtt gggaatgaaa ttttgcgtaa tttaacaaag attattaaga gcaatatatt
      241 tgaagaggaa atgtttgcaa gaatatctgc ggataatttt attataatta tgaaatatat
      301 agaagaagaa gatataacaa agaggttaaa aactatattt gaagaattag acttgtttaa
      361 taataatcaa gaagaaaaat ataagttagt tttaagttgt ggaatctatt ttatacttcc
      421 agaagataga gatatcaact caatcataga tagggctaat atacctcata aaatggcaaa
      481 gggaggacat aaaagttcat atgcatttta tgataataag attcatgacc aagagataaa
      541 agaaaaagag atggaaaata caatgttttc aagtctagaa aataaagaat ttattatata
      601 cttacaacct aagatagaac ttaatacagg agagatacaa ggttcagaag cacttgttag
      661 gtggaaacgt cctgataaag ggctaatacc tccaaatgaa tttatcccat tttttgaaag
      721 aaacggtttt gttataaatt tagatttata tgtgcttgaa gaagtatgta tataccttag
      781 aaaatggatt gatgcaggaa taaatccagt tactgtttca gttaatgttt caaggataca
      841 tttatattgc aataatttta tagaaacata caaaaatata attgataaat acaatatacc
      901 tgcaaagtat atagaacttg aattaacgga gagtatcata tttgataatt ttgatatttt
      961 aattgatata atgaataatt taaaaaaaat aggattttta atatctatgg atgattttgg
     1021 ttcagggtac tcatcattaa atatgttaaa agaaattcct atggatatct taaaacttga
     1081 ccaaaaattt attatggaaa cttataatag taaaagaagc aaaataattg ttactaaagt
     1141 aatagaaatg gcaaaagagt tgggtatgaa agtcatatca gaaggagttg agacagaaga
     1201 acagtttaaa cttttaaaag aagtaaagtg tgatatggca caaggatatt tgtttggaaa
     1261 gccaatgcca attgaagaat ttgaacattt aattgtatct aacttggtaa gagggtaatt
     1321 aatctgtaat ataaagaatt taataaattg tgttaaaaat ggactctaat gtagtgttca
     1381 ttttttttgt aaaatttttt atacttttac taaaaagtaa ttgacatata ctttgtatta
     1441 ttatattatt gtattaacaa cttaatacaa taatataata aggaggcatg aacatggaat
     1501 gggaacttga taataacaaa cctatatata tacaattagt agaacattta aagttaaaaa
     1561 taatttcagg ggaaattaaa ataggatcta aattagaaac agtgagagca ctagcagaag
     1621 atgcggaagt taatccaaat acaatgcaaa aggctctaac agaacttgaa agacaaggtt
     1681 tagtttatag tcagagaact aaaggaagat ttgtaacaga tgataaggaa aaaataaaag
     1741 ctatgaaaga ggaaatagct aatgtggaga taaatacatt aaaagtaacc ttagaaaagc
     1801 ttggatatga tagagatgaa atgctaaaac ttattacaga aaatctgaaa ggagaattat
     1861 aaaatggata aaaatataag caatgtttct caagatggaa tagttgaatt taaaggtgta
     1921 aacaagagtt atggaactaa gaatgtttta aaaaatattg atttaaatat accaaaggga
     1981 aaaatagtcg ggctgttagg acctaatgga agtggcaaaa gtacaatgat taagctaatg
     2041 aatggattat tacagcctga taatggtgaa attatgataa atggaatgaa accatccata
     2101 gatactaaaa aaatagtttc atatttacca gaaagaactt atttaaatga ttggatgaag
     2161 gtatcagatt tacttaagtt tttctatgat ttttatagtg attttgatgt tagaagagca
     2221 aatgagatga ttaaaagctt agatattgat gtaaatgaaa agctaaagac aatgtctaaa
     2281 ggaactaaag aaaaagttca acttatttta gtaatgtcaa gaaatgcgag catatatata
     2341 ttggatgaac ctataggtgg tgttgaccca gctgcaaggt catatatact taagactata
     2401 ttaaaaaatt attctgaaga tagtacttta ctgattgcta cccacttaat aagtgaaata
     2461 gaaaacatat gtgatgagat aatattcata tcaaaaggag aaatagtgtt acaaggagac
     2521 gttgaagcaa taagagaaga aaaaggaaaa tctatagatg cattatttag ggaggaattt
     2581 aaatgttagg taaattatta aaatatgagt taaaagcaag tggaagaata tttataccgt
     2641 tatatatagc catattgata gtagctgtat ttaatggaat atttatgaat acaaatatac
     2701 ttcaggtaca gggaatagga atactagtat taacaagtct ttttatggca ttaggagttt
     2761 taactattgt agttacaatt caaaggttta gaaaaaatct tttgggggat gaaggttatt
     2821 taatgtttac tcttcctgtt agcactagtt cacttatatt atctaaatgt ataacagcac
     2881 ttatatacgc tgtacttagt tttatagttg cagtattcac ttttggagtt cttatgcttt
     2941 ttggtacttc tggaatttta ctacctgaaa ttttagattt gtttaacact agttttaaat
     3001 ggatttctga aaacttttta gatattttat tattagtagt agtaatgttt atttcatatt
     3061 catcatttat tttgttatta tatacatcaa tctcaatggg acaattgcca aaattcaata
     3121 aacatagaaa catagtggca tttgcttctt ttatagctat aaatatagta atatcaattg
     3181 ttggagatgc agttggaagc atcttaccaa atgaagatac aaatatggta tatcatcttt
     3241 atcagagtcc tagttttatg cttgcaatac ttggtagtct tgtagttgca atagcgttat
     3301 ttttcgctac aaaatttata ttagataaaa aattaaattt agaataaatg tttttatatg
     3361 gagctatctt ttgagtaatg ttaaaagatg gctcttatta tttatgtagt taattcatgt
     3421 atataatatg aatagtgttt tatttctagt taaatatata catatgttgt ataatgatat
     3481 tgcgtgtaaa aatttgtaag aataactata cactttatat aagttatatc aagaaaattt
     3541 aaaaatatat ggatttgagg gagaagatat gaaaagttat acaaaggcta agtggagtgt
     3601 ttggggtata attacatttt catttgtttt agtattattt ttaaggatgt caactgctgt
     3661 tgtttcagac aatttagcaa atgagttagg ttttaattct atacaaattt caaatatagc
     3721 atccttttgc ctatatgcat atgcatttat gcaaatacct gcaggaattt tgattgataa
     3781 atatggagca agaaaaatta gcagtcttgg aataataatg gcaagtttag ggtcaatttt
     3841 atttggtttg attcaaagta tagaattagc atatatttct cgtgtaattg taggggcagg
     3901 aacatcagta atattacttt gtattttaaa aattcaaggg agatggttta ataagtcaga
     3961 atttgcttct gcaacagcta aattttcatt tgtaggaaat ttaggtggag ttcttgctac
     4021 ttttccactt gtgtttttgt ctgaacttgt tggttggaga aattcatttt tattaatcgg
     4081 tataattggt gttgtaattg gatgttttat gtatattatt gttagagata caccaaaaga
     4141 gtatggattc aatgtagata ctgaacccta tgaaaagtca gagaaggtta atattgttga
     4201 tggtattaag tctgttataa agaacaaatc aacatggtat aattcaatga taatgttctc
     4261 ttttgttggt cttacaagtg catttataag cttgtgggga gtaagataca taatggacgt
     4321 ttatggtgtg agtaagagtt tttctgcgtt tatagtatca ttttttacat atggatttat
     4381 atttggttca ataattatgg attttgtatt tgctaaaata aggtctagca aatttaatat
     4441 aataaaattt ggagcaatga tagatttgtt tatatggatt gtaattgtag ttgtatacca
     4501 ggtaaaacca ccaattatag ttttaccaat atcattcttt attatgggat gtatagttat
     4561 gtcacatcta caggttttta atgatgccaa gtataaaaat aaagaaattt attctggact
     4621 ggcaactagt gttattaata cttttgaatt tataggaagt ggaattatta atttgattat
     4681 agctatctct ttacaagtaa attcatataa tatagttgat ggatataaaa aaggttttgt
     4741 agtatttata gtactaagta ttataactat agtatcatct catatagggg taaaaaatga
     4801 tgattttaag gctatatagt gttttattaa tagtttaatt tagtattata ttttgcatat
     4861 atataacaaa tagtttgcat tttcctaata tgtataattg gaatataaat cttgtttact
     4921 atgtacaaat ggtattgtat tatattaaat agaaaataag aactttaaac tataatattg
     4981 gttctaaaat ataaggtaag gagtttttta tggatacgac gaaaatagat gatataagca
     5041 agttagattt aaataattta gatattaata agatggatat gggaggtata atggaaagtc
     5101 tgctagaatg ggctactaca agcggagtaa aacttataat aggcatactt atactttcta
     5161 ttggatttaa aataataaaa aaatttgtaa accatgttat gttattactt agtaaaagag
     5221 atattgattt aactttaaga aagtttttaa agtctcttct tttgagtgta cttaaagttg
     5281 ccgttataat cattgttctg gaatattggg gaatgagttt gtccagtttt gctgctgtga
     5341 tagcatcagc aggggtagca ataggtttgg ctttacaagg tagtctatca aattttgctg
     5401 gaggatttat tatactattg attagacctt ttaaagtagg agattacgta gaagcagcag
     5461 gtcatggagg aacggttgaa caaataggtc ttttttatac acaattggtt actccagata
     5521 ataaacagat acttatacct aatggttctg tatcaaatga tagtctaata aactattcag
     5581 caaagaatac tagacgagtt gatttgactt ttagtgtagg gtatgaagat gatatactac
     5641 atgttagaag agtacttaaa gatattgtta ataatcataa attaataata aatgaaccag
     5701 aaccatttat aggagtagta gaacatggag ataatgctat taaatttgca actagagttt
     5761 ggtgcaagac agaagattat tggacaattt atcttgattt actagaagag gttaaagtta
     5821 ggtttgacga agaaggaatt acgattccat atcctaaaat ggatttaacg gtaaaagaat
     5881 taaacaaaat ttaatttttt aatatagtgt ttaaatttaa gaatatcata tagatatact
     5941 tataaggttt gtaaaaaatt aataagtact gttattttgt aagtaaaaat ataaaaaaat
     6001 gggagtgtct caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat
     6061 atatttttat tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt
     6121 tttatgtaac tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt
     6181 tttaatataa aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat
     6241 ttaaggaggg tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg
     6301 ggtttattaa ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat
     6361 acaaaaaagg aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat
     6421 ttaatggaac aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa
     6481 tataaggatg ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat
     6541 actgtggaag gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa
     6601 gatgtattag aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga
     6661 aaagaaaaat tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta
     6721 aaagaacaaa atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt
     6781 tataaaataa gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag
     6841 ggaactgtat tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt
     6901 tcttttttga ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac
     6961 tattttagga ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt
     7021 gggtaggttc gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg
     7081 gaactactta taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag
     7141 ttaaagataa gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg
     7201 aaaaaataga ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa
     7261 agcttttgga tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc
     7321 ttaaggaaat cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat
     7381 caattgggga caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca
     7441 tatacacata tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact
     7501 atagtaatga taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg
     7561 ctttaagata ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta
     7621 tagaaaagat aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa
     7681 ctgaaaatcc tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa
     7741 agttagaaaa agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg
     7801 cagaagctat aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg
     7861 taatagagca taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat
     7921 ttggaacacc aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt
     7981 taaacccagt tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg
     8041 gagaaggtat agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac
     8101 cttttgctgt taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag
     8161 gtaaattcct agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta
     8221 aatgtgtaat ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat
     8281 gtggagctaa ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag
     8341 atactgatga atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg
     8401 ctataagaaa aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga
     8461 cttactatag acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca
     8521 tggaagataa agattggtat gataaaaata atatagtagt aatattagga acgaatgtag
     8581 ataaattaga tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata
     8641 agcttgtaat agctacagga agtagaaact ttataccacc aattaagggc catgatttag
     8701 aaaatgtgtt tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa
     8761 aatctaagaa agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat
     8821 ttagactaaa aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac
     8881 aacttgataa agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg
     8941 taagattggg tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat
     9001 ttaaagatgg agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa
     9061 acactcaaat ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga
     9121 cattacagac aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata
     9181 ttagtttagc aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa
     9241 gtggagataa tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg
     9301 taaaagtatt tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg
     9361 aaggtcaaag aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac
     9421 ttataaatga tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt
     9481 ttgaggatat tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt
     9541 gtctcaaaat tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta
     9601 tataaaataa catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt
     9661 tttatatgga ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta
     9721 ttgaaagtta atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac
     9781 aattaatctc agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct
     9841 tttcttataa aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat
     9901 attataaatg atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt
     9961 ttgattgtgg atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa
    10021 ggttttacag tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa
    10081 aatttggata tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc
    10141 cagaaaattc gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat
    10201 atagacaaga taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac
    10261 cctttggaag ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat
    10321 tcttatgaac aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat
    10381 aaagaaacac ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt
    10441 tctattcttt ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt
    10501 gaagctgtat ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg
    10561 cgattacgtg aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg
    10621 ggagtaggtt atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt
    10681 gagaaataaa atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg
    10741 gattttatat tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga
    10801 aaatgctttt cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa
    10861 tagcatggag ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt
    10921 ttatttaaat tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat
    10981 caaagaagat gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat
    11041 aaactcaatt aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg
    11101 taagaatgaa cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat
    11161 agggtatctg acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata
    11221 tttatctatt tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga
    11281 aattacaagg tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg
    11341 tatgcttgaa caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata
    11401 tacactgaat gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt
    11461 atttgataac ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat
    11521 aacagctgta caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat
    11581 tccagaggaa aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa
    11641 ttcaaacagt ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca
    11701 taacgggaca ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc
    11761 gattgtagga aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat
    11821 aaataaagag cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt
    11881 ttgatacagt aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa
    11941 ttgtaagtga aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga
    12001 ttgtataaaa ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca
    12061 gttatatttg gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta
    12121 cttgagaatt tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt
    12181 aattggtatc attatattgg agattataat aatatccaga ataacacttg gtttgaaagg
    12241 gatactgata taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa
    12301 tttttaattc ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt
    12361 gaaaatgata ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat
    12421 ggagaagatg gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt
    12481 ggtactcttt cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca
    12541 gaaggtatta aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct
    12601 gaactgtcaa aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca
    12661 tttggtatta gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt
    12721 gaacatgata atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca
    12781 gttttaggaa acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga
    12841 ttttttgatt atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga
    12901 attgatttgg attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt
    12961 ggatgttctg gatttgctag agtagatatg tttttaactc catctggtga aattattttt
    13021 aatgaagtca atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa
    13081 ggtattggat tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga
    13141 tgaataatat tttattagat aaaaataaag aagataaaat gacaaatact atcttattga
    13201 ctaaagataa agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa
    13261 catctgaaga tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac
    13321 gtgaagctac aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag
    13381 tacctgtaag tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc
    13441 gtgacaatgg aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc
    13501 agacagggct tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg
    13561 aatttcctta taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta
    13621 tagagcgtta tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt
    13681 ttcgttatgt gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag
    13741 aatatataga atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag
    13801 aaaaaattat agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta
    13861 tacctgaaca atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat
    13921 tttggagaaa tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta
    13981 tgcagggctt gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc
    14041 tccattatta tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat
    14101 tgctgtgcca ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa
    14161 ttgtaataaa ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt
    14221 aatttatatt cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa
    14281 tattattaag gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc
    14341 aattattggt gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact
    14401 agtaatcact attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt
    14461 agaaaaaata ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac
    14521 acgaaatggt atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa
    14581 atctaattat atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat
    14641 gttatgtgag ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat
    14701 tatgttattg ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg
    14761 agctaggtta ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt
    14821 tgttattcgt atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct
    14881 tattcattat atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat
    14941 tcttaaacct ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg
    15001 gattgaaatt gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc
    15061 agaagattgt gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga
    15121 agtagcgact tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga
    15181 ggggattgat ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa
    15241 cccaataagg gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa
    15301 tcatgctatc tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac
    15361 aggtatgcac agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa
    15421 attaaaatac ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa
    15481 agatgaagat gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt
    15541 tctgttaaaa aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt
    15601 taattatccc aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt
    15661 aagttctcca aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa
    15721 atcacaagta attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt
    15781 ctttattgca agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat
    15841 acctagaaat ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat
    15901 agtgggtcga atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga
    15961 agttggtaat acggcaataa taataggaag agataattta tctgaattat ctgcttctga
    16021 agttgcaaat aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt
    16081 aaatgttata aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt
    16141 ttactctgac tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca
    16201 tgacaattta agttataaaa aggtgatgtt tcagattttt att
//
LOCUS       DAELTA010000089        16120 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.089, whole genome shotgun sequence.
ACCESSION   DAELTA010000089 DAELTA010000000
VERSION     DAELTA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.089"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..670
                     /locus_tag="KPG13_002353"
     CDS             35..670
                     /locus_tag="KPG13_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986063.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            680..1384
                     /locus_tag="KPG13_002354"
     CDS             680..1384
                     /locus_tag="KPG13_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG0986064.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            1424..1882
                     /locus_tag="KPG13_002355"
     CDS             1424..1882
                     /locus_tag="KPG13_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG0986065.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            1901..2158
                     /locus_tag="KPG13_002356"
     CDS             1901..2158
                     /locus_tag="KPG13_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG0986066.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            2168..2953
                     /locus_tag="KPG13_002357"
     CDS             2168..2953
                     /locus_tag="KPG13_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG0986067.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            2988..3515
                     /locus_tag="KPG13_002358"
     CDS             2988..3515
                     /locus_tag="KPG13_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG0986068.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    3690..3922
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4015..7122
                     /locus_tag="KPG13_002359"
     CDS             4015..7122
                     /locus_tag="KPG13_002359"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG0986069.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     gene            7418..8290
                     /locus_tag="KPG13_002360"
     CDS             7418..8290
                     /locus_tag="KPG13_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0986070.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            8417..8689
                     /locus_tag="KPG13_002361"
     CDS             8417..8689
                     /locus_tag="KPG13_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986071.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(8852..9424)
                     /locus_tag="KPG13_002362"
     CDS             complement(8852..9424)
                     /locus_tag="KPG13_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0986072.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            9608..10450
                     /locus_tag="KPG13_002363"
     CDS             9608..10450
                     /locus_tag="KPG13_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG0986073.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(10553..11629)
                     /locus_tag="KPG13_002364"
     CDS             complement(10553..11629)
                     /locus_tag="KPG13_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG0986074.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            12126..13529
                     /locus_tag="KPG13_002365"
     CDS             12126..13529
                     /locus_tag="KPG13_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0986075.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    13620..13860
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13903..14601
                     /locus_tag="KPG13_002366"
     CDS             13903..14601
                     /locus_tag="KPG13_002366"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG0986076.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            14624..15625
                     /gene="trpS"
                     /locus_tag="KPG13_002367"
     CDS             14624..15625
                     /gene="trpS"
                     /locus_tag="KPG13_002367"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG0986077.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         6264 a         1786 c         2806 g         5264 t
ORIGIN      
        1 ttataatgaa agtaatttta tagggggaga gtctttgaaa aaggtaaaat ataccagatt
       61 agtgatactt ggtattctta tatatttagt atttaatata ttagtaatga taattagcaa
      121 aaatgtagat acgttggtgt taaaaaatga aaaagttgat gcaaagattc atgtaaaagg
      181 aactatagaa aaagatgcca atgaccaaag taattatcta tctacataca tcagtaaaga
      241 agattctaat aactttgaaa aaaatcaaaa tgtaataatt gaatatcatg agcaaaaact
      301 agatgcaaaa gtttacaaaa tatatgaaaa taataataaa ataatggtaa aattaaaaat
      361 aagtaaccaa attgttgcaa atcaggatac aagtgtcgaa gaatttgata taatatataa
      421 tcagatggaa tgtttaaaaa tacctaaaac ttctataaaa acaaaagaca ataaacgtgg
      481 tgtatatgta attgatgaac aatcacaaag tgtaaaattt gtcatattag aaggaataac
      541 ttatgagaat gaatcttctg tatttgtgga ttattataaa aatgacataa atggagttac
      601 aagtgttaat ctatatgaca aaataatttt aagacctaac agtataaaca caaatataag
      661 gataaagtag gtgataataa tgggttctat taaagataat ttgtctgaga taagaaacaa
      721 aattgataaa gctgcaaaaa aagtagatag agacagcaaa gagattaccc ttttggcagt
      781 cacaaagact gttgatgtag acttagttaa tgaggcaatg agggaaggaa tcacttctgt
      841 tggtgaaaat aaaccccaag aattagctag aaagtatgat attataggtg ataaattaaa
      901 ctggcacctg ataggaactt tgcaaaccaa taaagttaag tacataatcg ataaagtttg
      961 tatgatacac tctttagata gaattgcatt atgcgaagaa attcaaaaac gtgcagaaca
     1021 aataaacaga gaaatagatt gcttagtaca agtgaatata tctaaagaag agtctaagca
     1081 tggacttgat agagaagaag ttgtagactt tgtcaaagaa gtgtcagaaa aatataaaaa
     1141 tataaaaata aagggtctaa tgacaatggc accttttata gaagatgaag atgagataag
     1201 aggtgtattt aaaggtctta aagatttatc aaaacaaata aaagaactaa acatccctaa
     1261 tgtagggatg gatgttttgt caatgggaat gagtcacgat tatgaaattg ctatagagga
     1321 aggggctacc attgttagag ttggaacatc aatctttgga gaaagaaact acaataaaaa
     1381 ataattactc aaatttgaat aaattttaag gaggaagtta ataatgtcaa atggaataat
     1441 atctaaattt aagaattgga tagttgatga agaagaagat tacattgaag atgaatatga
     1501 aagtggcatg gacgatatag ttcaagaaga agaaatgaac agtggcttta gtactgcaaa
     1561 agcaaataaa attgtaaatt tacatacaac tagccaaatg aaagttgtaa tagttgaacc
     1621 taaggtttat gatgaagctg caactatagc agaccatcta aaacaaagaa gggcagttat
     1681 agtgaatttg gaaggattga ctaattctga agttcgtaaa tctatattta actttatgaa
     1741 tggggcagtt tatgtcttag atggaagtat acaaaaggtt tctaaatcaa tatttatact
     1801 agctcctaat aatgtggata tagatgcaaa tatgaaaaaa gaattagaaa gtaaagcatt
     1861 cttcccttgg caaaataaat agtaaaaagg cggttaatat atgggaacaa taagaattgc
     1921 actttattat cttttagata taatagcatg gatgatagtt ataaaaagct tgatgacttg
     1981 gtttccaaat ggaagacaaa gtaaaatttt tgatattttg gaaaatctta cagagccaat
     2041 agaagggcca ataaggtcta taatgtataa atatacaaat ggacctatag atttttctcc
     2101 tttaattgca atagtattat tgatgttcct aagacaatta gtattggtta tattctaagg
     2161 tgataggatg gataaattaa aactaacaaa tcacataaag gatatagact tgaaaaataa
     2221 aatgtttaaa gtgatagaca aggctaatag ttgtataaaa aactatgatg ttaagtctac
     2281 ggagtttctt aatccatatg aagtgaaaaa tgctgtagca atattaaatt ctacaaatga
     2341 aataaaatac agtgtggatg gaggttatga acaagctgaa agaagcactg tatttattta
     2401 tccattttac atggaatatg aagatataga agatactctt agatttttac aaatagaagg
     2461 gaatttcaaa tttaaaaata tatctcataa agattactta gggtcaatac tgggtctagg
     2521 tataaaaaga gaaaaaattg gagatataat tatacatgat agcttttgtc aagtagttgt
     2581 aagtagtgat atttgcgatt ttataattgt aaacttagaa aaggtttcaa gaaataatgt
     2641 aattgtaaag gaaatttcca gagaaaatat tgttaatagt tcttcaaaat ataaagaagt
     2701 atcatttaca gtatcttcag ataggttaga ttgtgtgata agtggtattt acaatatatc
     2761 tagacaagat agtgcaaaat acataaatgg agaaaaagtc catgtcaatt atgaaaagat
     2821 aacttcaact tctaaaatag taaaaaacga tgatttgatt tctattagag gcaaaggaag
     2881 agctaaggta acacaaatag gagatattac taaaaaagga aaaataaaag ttcaagcaag
     2941 attaattgta taaggactaa tttaaattat agctgggagg aaatgtaatg ctaactccaa
     3001 ttgagataga aaataaagag tttaaaaaag gtttaagagg gtatagagat gaagaggttg
     3061 atgaattttt agatatcatc aaggaagatt atgaatctct atgtagggaa aatacagctc
     3121 ttaaagaaaa attagggtta tatcaagacc aagtaaacaa gtatgaaaat attgaagaaa
     3181 cactaaaagc tactttaata actgctcaaa gtgcagcaga agacacttgt agcgcggcta
     3241 ataaaaaggc taagataatt gttgaagaag ctgatttaaa agctagacaa attatagaac
     3301 aagctaataa ccgtgttata gagataagaa aagagtatga tgctttagtc aaagaattta
     3361 agatatttag aaataaattt aaatcactgt tagaagatga aataagaagt gttgatgaga
     3421 ttttttatga tgtagatgaa aaatgtgtaa atgcatttga aggaacaaca gtgtataatt
     3481 atagtaatga tgaagctgct acaactttag aataagcttg taatttgttt attttttatg
     3541 tagaataatt gtgtgagttt gagaaatcta taacaaagat taaaaatagg ataaaaatgt
     3601 aagaaaaata gaaattatta tatttttcaa aaatatattg actattcaaa ttatttgtta
     3661 tagaatttca tataaacaca gataaatttt taaaagcgtt gatagagaca tttatcttta
     3721 gataatttta cagagaattg ggtatgctga gaaccaatga atttataaag ataaaaatca
     3781 ctcttgagct gcaggctgaa cgattaaatt ctactaagct gtgccggtta gtaccgttat
     3841 actattagag tgttatatgt agctagtatt tagtttacat atagaattag ggtggtaacg
     3901 cgatttataa ctcgtccctt tggtttaaag ggggcgagtt ttttagtatt taaatttatg
     3961 tgaaagaata aaaaattttg gaattataat atttagtagg aaagggtgat ttacatggcg
     4021 aaatttaagc cattggtaga cagttctgta aaacaagcag aagcacaagt ttttgattat
     4081 tggaaagaca taaatatact tgaaaaaact ttagaaaaag ggaaagatga cccaagtttc
     4141 gtattctatg aaggacctcc aacagctaat ggaaatcctg gagtacacca tgttttatct
     4201 agaacgctta aagattcagt ttgtagatat aaaacaatgt ctggttatca agtaaagaga
     4261 aaagctggtt gggatacaca tggattacca gttgaaatac aagtagagaa agagttaggt
     4321 cttactagca agcaacaaat agaagaatat ggaatagctg aatttaacca aaaatgtaga
     4381 gagtcagttt tctcatttga aaaacaatgg agaataatga ctgagagaat ggcttatgaa
     4441 gttgatttag ataatccata tataacttta gataataact atatagagtc tgtatggtgg
     4501 atactaaaca aattcaacaa agaaggatat atatatgaag gacataaaat acttccttat
     4561 tgcccaagat gtggaactgg tcttgctagt catgaggttg cacaaggata taaagaagta
     4621 aaaactaata cagttatagc taaatttaag aaaaaagatg ctgatgaata cttcttagca
     4681 tggacaacaa ctccatggac tttaccatca aacgtttcat taacagtaaa tgctgatgta
     4741 gattatttaa aagttaaaaa aggtgatgag gtatattatg tatctaaacc tttagctgat
     4801 aaagtactag gtgaagatta tgaagtttta gaagaaatga aaggtaaaga cctagaaggt
     4861 ttagaatatg aacaattaat gccttttgta gaagttgaca aaaaagcatt ttttattaca
     4921 gtaggagatt atgtaactac tgaagatgga actggtatag ttcatacagc accagcattt
     4981 ggtgaagatg actacaacac aggtaaaaga tatgatttac cagttattca accagtagat
     5041 gaaactggta aatttacatc tactccttgg gaaggtagat ttgtaatgga agatgggcta
     5101 gatgttgaaa taataaaatg gctagcttct gaaaataaat tatatagcaa agaaaaagta
     5161 gttcataatt atccacactg ctggagatgt caaactccat tagtatacta tgcaaaacca
     5221 agctggtata tagagatgac taaacttaaa gataaattaa tagaaaataa taatggtgta
     5281 aaatggttcc caagttttgt aggagagaag agatttggaa actggttaga aaatctaaat
     5341 gactgggcaa tatcaagaac tagatattgg ggaactccac ttcctatatg gagatgtgaa
     5401 tgtggtcata cagattcagt tggttcaaga gctgaattag ctgaaaaagc tatagaagat
     5461 gtaaatccag aaacagttga acttcataga ccatatgtag atgatataca cttaaaatgt
     5521 gaaaaatgtg gaaaaccaat gacaagagta acagaggtta tagactgttg gtttgatagt
     5581 ggggctatgc catttgctca acaccactat ccatttgaaa ataaggaaaa ctttgaccaa
     5641 ttgttcccag ctgactatat atcagaaggt atagaccaga ctagaggttg gttctactca
     5701 ttacttgcag tatcaacatt tgtattagga aaagcaccat ataagagtgt tttagttcca
     5761 gatcttgtac ttgataaaga tggtaagaaa atgagtaaat caagaggaaa tacagtaaat
     5821 ccaatggaat tatttgacca atatggagca gatgcattaa gatggtattt actatatgtt
     5881 tctccaccat ggactccaac taaatttgat atggatggat taaaagaaat ccaaagtaaa
     5941 tttataggta ctatgaagaa tgtatataac ttctttactt tatatgcaaa cacagatgat
     6001 ataaatccaa ctgaattttt tgtagaatat aaagatagac cagaacttga tagatggata
     6061 ttatctaagt tcaataattt aatgaaagat gtagaagaaa atttagctat atttgagtta
     6121 aataaaactt taagaatgat acaagatttt ataaatgatg atttatctaa ctggtatata
     6181 agacgttcaa gaagaagatt ctgggcaact gaattaacag aagataaaaa agcagtgtac
     6241 aatacaactt atgaattgtt acatggtctt tgtagagcta tagctccatt tgcaccatat
     6301 atgtcagaag aaatgtatag aaacttaaca ggagaagtat ctgttcattt agctgaatat
     6361 cctaaatgta atgaagagtt agtagatact aaattagaag aaaaaatgga tttagctaaa
     6421 aacttagtta ctttaggtag agcttcaaga gaagtagaaa gaataaaagt tcgtcaacca
     6481 ttacaaaaag tgttagtaga tggtaagttt gaagatacaa taagtgatgt agttgattta
     6541 ataaaagaag agcttaatgt taaagaggta atatttgcta aggatttaga tgagtatatg
     6601 aactttagtt taaaaccaaa ctttaaggaa gcagggccat tattaggttc taagatgaat
     6661 ttattcgttg gagcattaag taaattaaat gctcatgaaa ctgctaataa acttgaaaaa
     6721 ggtgagactt taacagttga tttagatggt gaagcttttg aatttaataa ggatttagta
     6781 ttaataggta taactgctaa ggaaggattt aatgtatctg ttgaaaacaa tctttttgtt
     6841 atactagata ctaagctaac tgaagagtta attaatgaag gttatgctag agaatttata
     6901 tctaaagtac agcaattaag aaaatctaat aactttgaag tattagataa tatagtaata
     6961 gaatactgtg gtgatgatga aattgctaag gctgttgacc attttaatga atatatcaag
     7021 tctgaaactt tagctttaga aataaacaga gtagatgata aatctttaga ggaacaaaac
     7081 ttaaatgacc atatgacagg tataaaagtt ataaaaaaat aaaaataata gttagttaac
     7141 caaaatccct atagaaatta gtttatagat ttctataggg attttttatg taatataatt
     7201 atctattcaa taaaatgttt taatattcca aaatttacaa taaataaatc tatataaatc
     7261 atataaatta tttatgtcat gtgatagaat attaatgaat aaacgtttaa gtgaggatga
     7321 ataatatttt taggaaacta cttctgataa atttatttgt aaatttatcg tgtgttatgc
     7381 agggtggttt atttattatg caaaggggtg cgttttgatg tatggtgaaa gtaaaaagct
     7441 aagtattgat aataatatat ttttacaata ttgtcttatt atattgggta tgattctatc
     7501 aaccttagga ataaatttat atctagcacc agccaagtta ttaagtggag gagtagctgg
     7561 aatttgtgtt atattataca aattatttgg tattaatcag ggaatatcaa gtttcttgat
     7621 gaacatacca atatttatta tagctagaaa gtattttgat aataaatttt tgctaataag
     7681 ttttgtcaat atgttactat tttctctagc actggggcta acacaagata ttgctaaata
     7741 tttcccaata gatgatacta tgctacagtg tatttatggt ggtgcattaa ctggtatagg
     7801 tatggggtta acttttaaag caagagctac tgctggagga cttgatataa tagcagccat
     7861 aatgaaaaga aagtatgata tacctatgaa aaatacattt ttatttatta atttttttgt
     7921 agtatgtgca ggagcttttt tatttggagc taaattggtg atgtacactt taattacaat
     7981 gtatataatt tcttttacta tggatatagg aaaagattgc tttgatagaa aaaaatctat
     8041 acttttaata tcaaataaat atgatgaaat atccaaagtg ataatgaata aaatgggtag
     8101 aggagtcact tttttggagg cagaaggtgc atatacacaa aataagaaaa agatgattta
     8161 ttgcataata tctgcaaatg aaataggtaa atttaaagat ttaatttatg atatggatag
     8221 tgaagctttt atttctgtca ataacgttga agaagtaaaa ggtggaggtt ttaaggataa
     8281 atttctttaa gattatatat gttaattata tatgttaaaa aatgccatat gtattgagat
     8341 tatacctagt tagatgtata ctgtagttaa tggggtacat gaagtactca ataatatact
     8401 aggaggtata agaattatgt taagagagag atttgtagta agaaatcatc ataatccaca
     8461 tacaagtggg tgttgtggga ctggaggaga aggatgctgt ggaggagata accatgaaca
     8521 ttcacatcaa cattctcaca gtcatgggca tgaacatgag catgtaaatg gtgtaggtca
     8581 cgaccataat catgatgatg gtttttcagg tactcatgag catagccatg agcatacaca
     8641 tacacatggt catgaacatt cagatgggca tacacatgaa cataaataaa aacattttaa
     8701 atttataaga gatttttatt ttgtgattcc atgaaggttt aaataaaaat tgaatgtttt
     8761 aagtaaaaat tattgagaat aaataagaaa gtgaactgtt aaagtagata acttaaatag
     8821 ttcactttta ttaattatct aagtattaag tttaaatcca atgattaact aaaaaagaag
     8881 ctatattttc ataaggtatt ttttctgttt ttaataaact ttggacaact aaaccatcaa
     8941 ctattgacat gagtaaaaaa gaaaatgatt cagcatcctg ttcattttcg ctactattat
     9001 atttttctag tgattcaaca atttcagatt taagagtagt tctccattca atatatttta
     9061 atcttattct ttcctttaaa tcattatttg aagtaattgc ctcacaaata agatacatat
     9121 gtaccctacc acgactttct atagtagata ttttctctaa gattaagtta accatttcta
     9181 tttgagaatt ttttgatttg acatttttta cacagtttag tatagcacta gttataactt
     9241 ctaaatgagt atccgcaata tcatatataa tatcatcttt agatgcgtag tgataataca
     9301 aagtaccttt acttatattt acatcttttg caatatcagc aaggctagta ttttgaactc
     9361 catttttggc tattaaattg gttgcagatg ataaaatagc ctgtttcata ttatttgttt
     9421 tcataaaacc tacctttcca tgtatattta taacaaatta ataacattat aagcttatgg
     9481 atatttattg tcaataaagc gagaatctat atctattgac tttttttcat tcaatatata
     9541 aaataataat taaatcggtc gtacgaccga ctgaataaat aaaataatat cttatagtga
     9601 ggttaatatg gataaaataa aattgatagt ggacagtgca tgtgacttac ctgatgatat
     9661 aatagaaaaa tacaacatag aagttgttgg tttaaatgta tcttttggag aagaaagtta
     9721 tatatctgga aaggaaatag ataatgaaac tttctataga aaaatgagtg agagtaaagt
     9781 actaccaaaa acatcatgtc cttctcctga taaattttta gaagcatacc attgtcagga
     9841 agaaagtgta ttagtgatta atataagttc aggattatct gggacttata atagtgcact
     9901 tttagcaaaa gatatgtttg aaaaggaagg aaataacaag aaagttgagg ttatagattc
     9961 acttagtggg tcaattggaa taggacaatt agtgttaaaa gcagcaatat taataaaaga
    10021 aggaaaagaa attgaagaga ttgtttcaat aataaataaa tataaagata atcctccatt
    10081 ttttggaaca cttgaaactt tagaaaatgc tattaagggt ggtagaataa atccaatagc
    10141 aggaaaaatt ataaatacct taaattttaa ggcgataata caagttgcag atggcgttgt
    10201 aactcctata gataaggcaa gaggtgaagg aaacagtttg aaaaagctga ttactttagt
    10261 agagtcaaaa attaaagata aagaagaaaa ggttttattt ataggacatg ctaattgccc
    10321 agaaaaggct caaaaggtaa gggaagttat ggaaaaggat gtaaaataca aagatgttgt
    10381 aatctgtgaa attggttcag tgatggggac atatacttca aagggtgcaa tcttaataac
    10441 tgcaatataa ataatggatt gaaataaatc atagattgag aaattaaagc catataaaaa
    10501 tgtttaaaac atttttatat ggctttttaa ttttatcaaa ttgattttag aattaaccaa
    10561 ctacacatat ttctttgtca tgtttgttta atatctcaca accatcttca gtaacaagta
    10621 ctaaatcttc tattctaact ccgaactcac ctggtaaata tatacctggc tctatagaga
    10681 atatcattcc tggttgtaca gtatctgtat ttatagaaga tacatctcct ttatcatgaa
    10741 cttcaagtcc tatagagtga ccagttctat gagtgaagta ctgtccatat cccttttcag
    10801 ttatgtagtc acgagcagca gcatctatat cacagaatct aacacctggt tttacaatag
    10861 cttcagctct cttattagct tcaagaacta tttcaaatat ttctctacct ttttcaggta
    10921 tttctttata gaatacagtt cttgtcatat cagcacaata gttatctttt atacaaccta
    10981 tatctaagat tattgcatct cctggtttca caagtgcatt tccaggttca ccatgtggat
    11041 tagctccatt aggtccaaaa cctataatag ggtcaaatga aaatccatca gtacctaggt
    11101 cttcatatat tttagataat ttaccaacaa gttgtttttc tgtaagttca ccagaaacag
    11161 tagctttaag ttgttccata gccttgtcat ttaattttga agctattctc attaactctt
    11221 tttcttcttc atctttacac attcttaaag tatctattat ataagatgaa tttacaaact
    11281 tacttcctcc acctaattct attaagttta ataagaatct agctggccag tttttatcaa
    11341 cacccatagt agcatttttg tctatatgtt cagttattat ttctactgga ttttgagtat
    11401 cattaaacca aaccatttca acacctaaat cttctgaaac tgggaataat tcatttataa
    11461 ataatttatt attgccattt aaatttaagt ataatgctaa tagtctttcg cctggatgga
    11521 ttagtactcc tgtaagataa aatatagaag tagggtcact tactaacatt tgagaaatat
    11581 catctttttt catttgttca agtacagcat ttaatctttg agctttcata ataatttcct
    11641 cctaatcaaa attcaataca aatcaatttt agcattatta aacaaaaaag agtatacttt
    11701 tcttctagaa atataaaaaa aatttgttat atatttttta tattaagttt aatttctact
    11761 tttgcattaa atacggaagt aaaatgactt tttattgtga aaattctata aaagtaataa
    11821 attaagtcta taaagttatg tattttatct ttttatatac taatgaaaaa taataaataa
    11881 aattttataa taaaataata ttgaaacaat tttaaaaatt tagtatcatt agaattaaag
    11941 tggtaaaact tttaaattta tcatttttaa taagatttga tttcaaagta tttaaggttt
    12001 attattaaaa ctgtttttga atatgcaaat attttgaaaa atatcagttt aataataatg
    12061 aatctatttt attaattttg aaattaaatt taaaataatt aatagagagg ggaaattaaa
    12121 ttaggatgga aaagaaaaaa ataggcctat ggagtttggt atttatgaat gtatcagctt
    12181 tatatggtat aagatggata gctaaatcta cagcatcaag ctttggttta ggattaggtg
    12241 caatacctat gtggttatta ttctcattta tatactttgt accaggagct ttaatttgtg
    12301 ctgaattagc ttctacatat caaaaaggtg atggaggtct ttacgattgg gttaaggaag
    12361 cctttggaga aaagtatgga tttttagttt cttggttaaa ctggacagct aaaatatttt
    12421 ggtattcttc atttttaact ttcttagcag taaatgtttc ttatacaata ggaaatccag
    12481 cccttgctga taataagatg tttgtactta tattatcttt aataatattc tgggcattat
    12541 ctcttatagc aactaaagga ataagtttta caaaaatatt tactaataca ggtgcattag
    12601 gttcaacaat accaagtgct atacttataa tattttcatt tgtagcagta tttgtgttga
    12661 aaaaacatga tatagcttct acatacacaa tagcttcaat gactccaaaa cttaatatgg
    12721 atgcatttgt tgctatatca gctataatgt ttggtttagc aggagcagag actgctgcta
    12781 acttcataac agaaatagat aagcctgaaa agaacttccc taaggcaata ttaatatcag
    12841 ctggaatagt agcttcatta tatgtattag gttcaattgc aataacaatg ataataccac
    12901 cagaccaaat tactgcttct aaaggtgtat tagatgcact ttctgctgtt tgtgcgtctt
    12961 taggaattgg ttcttggttg gttcaattaa tagcttttgg tatagctttt tcagtattag
    13021 gagcaatagt tctttacata gcatcaccaa taaaaatgtt atttggtagt gttaaaaaag
    13081 gaatatttcc agatagttta gtagaggtaa atgaacacaa gataccttct aaagcagtta
    13141 tacttcaagc aataatagtt actataatac tattggtgac tacattaatg ccttctgttg
    13201 atgctatata caatgttctt gtaactatga cagcattaac tgcattattc ccatatgtat
    13261 tgttatatgc ttcttatatt aaactaagaa aagagagacc agatgaaatt agaccttata
    13321 ctatggcaaa atcaacaagt acttgcctag gtttagcaaa aatggtttta gttgtaactg
    13381 tagtgggtat attattatct gcagcaccag ttatgcctac atttgctgaa aatgttatat
    13441 atgagataga gatgattggt ggaggtcttc tagttatatt atctggtcta tggttatgga
    13501 atagatatga aaagaaaaca gctaaataaa atgtatttta aaaatttctt ttggatatta
    13561 taatatatag ttgacaattt atagtgtaag gaatattata aaataaagaa attaataaat
    13621 taaatcctga gataagaaat agtaaataaa tatatttttg cacagagaat taaggatgct
    13681 gagaacttaa taaaaagtat ttattgaatg ggtctttgag gagtaaaatg aaatgtaatt
    13741 taatattaca gagtatttga tatcgtgtat gcacgttata gcatatatta agatatatat
    13801 attcaatttt gagtgtatat aaatttaggt ggtaccacgg aaaatatatc cgtcctattg
    13861 atttaggacg gattttttaa tatagaaaat ttaggaggaa aaatgaacgt aataggaatt
    13921 ataggtgcaa tggatgaaga agttagtatt ttagttgatt taatggatat tagagagact
    13981 ataaaaaaag ctagcttaga attttataag ggaattttgg aaggtaagaa tgtagtgtta
    14041 gtaaagtgtg gcataggtaa ggttaactct gctctttgtg cccagatact aataagcgaa
    14101 tttaaggttg atgctattgt taatacaggt gttgcaggag ctttaaacga aaaacttgat
    14161 gttaatgata tagtaatatc aactgatgct atacagtatg atgtagacac tacagcattt
    14221 ggagacccta agggagttat accaagaatg aagacttctg tatttaaagc agatgaaaga
    14281 cttatagatg ctgcttacaa atcttctgtg gaagaagtaa agactcataa agtattaaaa
    14341 ggaagagtag ttacaggaga taaatttata aattctaaag aattaaaaga agaattagta
    14401 aatgattttg gtggatattg tggagaaatg gaaggtggag ctatagctca tgtatgctat
    14461 ttaaataata ctccatttgt aataataaga gcaatgtctg ataaggcaga tggaagtgct
    14521 gatgttactt atgatgtatt tgtacatgat gctgccaata actctaaaga catagtttta
    14581 aatatgttaa aatcaatata aatatataag gaggtacaaa aaaatgagtg aaaaaaaagt
    14641 tatatttagt ggtgcacagc catcaggcaa aatgacttta ggaaattatt taggagctat
    14701 aaaaaactgg actgaacttc aagacaatta tgattgttat tatagtatag ttgatttgca
    14761 tgcaataaca gtacctcaag accctaaagt tttaagagcc aatactatag aattacttgc
    14821 tcaatatata gcttgtggat tagacccaga aaaaaatact atatttatac aatcacatgt
    14881 caaagagcat gttgaactta tgtgggtatt gaatactatg acttatatgg gtgaattaag
    14941 tagaatgact caattcaaag ataaatcaca aaagagtgaa gcaaatctaa atgcaggttt
    15001 attcacatat ccagttctta tggctgctga catattgttg tatcaaactg acctagttcc
    15061 agttggagat gaccaaaaac aacatttaga attagctcgt gatttagcaa atagatttaa
    15121 taatagattt tcacctactt ttgtagttcc agaaggatac tacccaaaag gtggagcaag
    15181 agtgatgagt ttgcaagagc caactaaaaa aatgagtaaa tccgattcaa atgaaaatgc
    15241 atttatacta ttagcagatg attcagattc tataaagaga aagataaaaa gaagtgttac
    15301 tgattcttta ggtgttgtaa aatacaatga tgaacaacca ggtattaaaa atctaattga
    15361 tatatattct aatttaagta aaaagagtgt agaagagatt gtaaacatgt atgaaggaaa
    15421 gggttatggc atatttaaag aagatgtggc tgaagtagta tcagaagctt taagacctat
    15481 aagagaaaaa tatgttgacc ttcttaataa taaagattat ttagaaaaaa tatatagtat
    15541 gggagcagaa aaagccgaaa aacaagctag aaaaactctt agaaaagttt ataaaaaagt
    15601 tggatttata gagagaagat actaattatt agctatcttt acttgataaa taaattttaa
    15661 agttattaaa aaggtatatg atatgaaaga gagagtctca aagtgaactt ttgttcatga
    15721 ggcactcttt tttgtatgaa ataaaatata ttttaatcat ttcaacaatg aaaaagagga
    15781 ttatctctat tttgagacag catcttttga taataaaaaa agatgagctt ttgctcatct
    15841 caaaggggtt agattattct tactttgggg aaagtagaat aaaggtaaaa gttaactgat
    15901 gtcaactttg tcttttttgc atatcactac tgagcataaa aaaatcagaa aaccatagcc
    15961 ttctgaaaca acttctaaca tacattgtat tttataccca atatgtaatt tatgagttaa
    16021 ttttaactct tataatgaaa attgtcaata atatatcaaa aaaatatttt ttaaatttat
    16081 ataattaaat gaatttatgt ttatatatag gtgatatgat
//
LOCUS       DAELTA010000090        15978 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.090, whole genome shotgun sequence.
ACCESSION   DAELTA010000090 DAELTA010000000
VERSION     DAELTA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.090"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="KPG13_002368"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="KPG13_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG0986078.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="KPG13_002369"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="KPG13_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG0986079.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="KPG13_002370"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="KPG13_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG0986080.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="KPG13_002371"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="KPG13_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG0986081.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="KPG13_002372"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="KPG13_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG0986082.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="KPG13_002373"
     CDS             2662..3168
                     /locus_tag="KPG13_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG0986083.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="KPG13_002374"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="KPG13_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG0986084.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="KPG13_002375"
     CDS             3982..4770
                     /locus_tag="KPG13_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG0986085.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="KPG13_002376"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="KPG13_002376"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG0986086.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="KPG13_002377"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="KPG13_002377"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG0986087.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="KPG13_002378"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="KPG13_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG0986088.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="KPG13_002379"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="KPG13_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG0986089.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="KPG13_002380"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="KPG13_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG0986090.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6258 a         1989 c         3249 g         4482 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaaatgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAELTA010000091        15926 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.091, whole genome shotgun sequence.
ACCESSION   DAELTA010000091 DAELTA010000000
VERSION     DAELTA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.091"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..996
                     /locus_tag="KPG13_002381"
     CDS             97..996
                     /locus_tag="KPG13_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG0986091.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            1020..2468
                     /gene="ddlR"
                     /locus_tag="KPG13_002382"
     CDS             1020..2468
                     /gene="ddlR"
                     /locus_tag="KPG13_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG0986092.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            2527..3333
                     /locus_tag="KPG13_002383"
     CDS             2527..3333
                     /locus_tag="KPG13_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0986093.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            3359..5278
                     /locus_tag="KPG13_002384"
     CDS             3359..5278
                     /locus_tag="KPG13_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0986094.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(5520..5867)
                     /locus_tag="KPG13_002385"
     CDS             complement(5520..5867)
                     /locus_tag="KPG13_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG0986095.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(5854..7782)
                     /locus_tag="KPG13_002386"
     CDS             complement(5854..7782)
                     /locus_tag="KPG13_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG0986096.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(8194..10383)
                     /locus_tag="KPG13_002387"
     CDS             complement(8194..10383)
                     /locus_tag="KPG13_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG0986097.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            10714..11445
                     /locus_tag="KPG13_002388"
     CDS             10714..11445
                     /locus_tag="KPG13_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0986098.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            11525..12160
                     /locus_tag="KPG13_002389"
     CDS             11525..12160
                     /locus_tag="KPG13_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG0986099.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(12411..13004)
                     /locus_tag="KPG13_002390"
     CDS             complement(12411..13004)
                     /locus_tag="KPG13_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG0986100.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(13075..13938)
                     /locus_tag="KPG13_002391"
     CDS             complement(13075..13938)
                     /locus_tag="KPG13_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG0986101.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            14101..15270
                     /locus_tag="KPG13_002392"
     CDS             14101..15270
                     /locus_tag="KPG13_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0986102.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            15371..15847
                     /locus_tag="KPG13_002393"
     CDS             15371..15847
                     /locus_tag="KPG13_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0986103.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         5869 a         2347 c         2101 g         5609 t
ORIGIN      
        1 gtggtacttt catcctcaac tgttaattca tataattaag tatgtaaata aaaagtttac
       61 tttaaaataa taaaaattaa aataggagtg tataatatga aaatagcagt tataatgggt
      121 ggaatatcat cagagagaga agtttcttta aattcaggaa aagagatata taataacctt
      181 gataaaaata aatatgaagt tgtaaaggta ataatagatg ataaaaaaga tatatttaca
      241 aagataccag aagatataga ttttgcaata cttgctttac atggaaaatt tggtgaagat
      301 ggatgtatac aatcaatact tgagactatg gatataccat actctggatg tggacctctt
      361 tgcagtggga tgtgtatgga taaaaatata actaaaaaaa tgcttagaga ttcaaaccta
      421 cctactgctc catgggtttt agtaaaatca gttgatgaaa tagactatga tgaaatagat
      481 aatataggtt atccagtatt tataaaacca aactcaggag gttcaagtgt agccacattc
      541 tttattcatt ctaaggatga agttgaagaa gcagttagaa aaggtctgga agttgatgaa
      601 tttgtaatga ttgaaaaata tataccaggt ggagaatata cttcattcat attaaatgga
      661 gaagtgttcc ctacaatatc tataaaatct gattcaggat tctttgatta tgaagctaaa
      721 tactcagttg aaaaaggtgc aaaggaagaa gttgtttact tagatgaaga acttcaaaaa
      781 agagtaaacg aaatttctga gacttgttgg aaaattttta attgcaaagc atatgtaaga
      841 gttgatatga ttataagtga aggtattcca tatgttcttg aactaaatac tcttcctgga
      901 atgactcaaa ctagtttaat tcctagaagt gctgctgcta gaggtattaa gtacagtgaa
      961 ctattagata aacttataga atactcttta aattagatga aataatttga ggtggtaatt
     1021 tgatattttc aaatctaaaa ttaaatgata atgaaccaat ttatatacaa ctaaaaaatt
     1081 atataagtga tatgatatca aaaggactaa ttccagacaa tagtaagctc ccttctacaa
     1141 gggagcttag tcagctttta caagttagcc gaaattctgt agttttaact tatgaagaat
     1201 taaaatcaga aggattaatc tactctattt caggaaaagg tacatttgtt aaatcaaaga
     1261 ataagtcttc aaatactaca tggtcattaa actgggattg tttagagaat acatactcta
     1321 aaaaagccaa tgaattggac attataaaaa gtgaaattcc ttggagctct gacctgattt
     1381 cctttaaaag tatttctcca gatggagatt tattcgacat ggaagaatta aaaaaatcct
     1441 ttttaaatag aatttcacta gaaggacata aattactcaa ctatggatat gcacaaggtt
     1501 ataagccact tattgactat ttactagaat atatgacaaa caaaggtgct gatataagta
     1561 ataaagatat actaataaca aatggattca cagaaggctt tgatattatt atgtcatctt
     1621 ttacccaaga aaaagactac ataatatgtg aaaatcctac tcataatact gctataaaaa
     1681 ttatgaaatc tcataacctc aatatcttag gtgtagatat aaatgaagat ggtttggact
     1741 tcaatatgct agaagacaat ttagttaagt ataaaaataa aataaagttt gcatacatta
     1801 caccctctta tcacaatcct acaggaattg taatgactcc tgaaaatagg tacaaattct
     1861 ataatcttat gaaaaaatac aatatagcaa ttatagaaga tggatttaat gaagaattac
     1921 tctacaatag ttctcatata tttcctatct gttccctaga taatatgaat aatggggtcg
     1981 tatatattgg tagtttttca aaaattttat ttcctggaat gagaattgga tggatatttg
     2041 cagataaaaa agtaatcaat aagctagaaa gtgtaaaaag gtgcagaaac atacatgtat
     2101 cctttttaga ccaaggaatt ttatttgatt atttaagtaa tggaggtttt gaaaaataca
     2161 ttaaaaaaat acgtaaattc tatggagaca aatttaactt tgcatataaa tgtataaaaa
     2221 aatatataaa aacagaatat atcttgggtg atggtggttt gcatatattt ataaaactaa
     2281 aaaatataga tactagagtt ttattagaaa aatgttatga aaaaaatgtt atatttatgc
     2341 ctggagactt attttttaca gataactctg gttttgatac tttaaggctc ggattttcta
     2401 ggttgtcttt tgaagacatt gaaattggaa taaaaataat tggagaaact ataaatgaaa
     2461 tgttctaggt taactaaata ttgttagcct agtttatttt tatgaggtat aatatatata
     2521 gtttatatga atctaatact gggggataat atgagtaaaa agtcaaaacg aagaaaaatt
     2581 aataaattaa aattaacgat tgtaataact gtattatttc ttgctttatt atctatatat
     2641 gagatactgt tctctaatcc caacaaaaaa ggtaatataa agagtcaaga aactatgcaa
     2701 aataaaaata atacaacaga tgattctaaa aataatcaaa gctctaccaa aaagcaggaa
     2761 gataataata tcaaacctac tttttcatat tcttcaatac cagataatat aaaaaataaa
     2821 atgattggta aatctatgcc tactgatgaa cctataagct ttgatagtct atcttattta
     2881 aaacttacat attatggatt tgatgaaaaa actcatcaag gagaaatgat agtaaacagt
     2941 gaactagctc cagaagtagt ggatattttt aaggaattat acgaaaaaaa atatccaatt
     3001 gaaaaaatta agttaataga tgactatgat gctgtagatg aaaaatctat gtctgataat
     3061 aatacctcat cattttgtta tagaactata gctggtacaa atgtagtttc aaatcatggt
     3121 aaaggacgtg ctatagatat aaacccatta cagaatcctc aagtttctgg aaatgatgtt
     3181 acacctaaag taagtacagt ttatgctgat aggtctagca caaaatttgg aatgataaaa
     3241 aaaggtgatg attgttataa tgcttttgta agcagaggtt ggagttgggg tggctattgg
     3301 aaaaacccag attatcaaca ttttgaaaaa taatagatta gaaataaagg tgaattatat
     3361 gaatttaatg acattggaaa atataagtaa aagctattct gaaaaaaaat tactagaaaa
     3421 tatatctctt ggaataaatg aaggggaaaa aataggattt attggtgtaa atggtactgg
     3481 taaatctacc ttactcaaaa ttattgcagg agttgaagaa agtgagactg gtaatataac
     3541 aaaaactaat ggtattagga tagaatatct acctcaaaat ccaacttaca atgaaaattc
     3601 aactgtactt gaacaagtat ttaatggaac ttctgaagaa ttaaagctac ttggtgaata
     3661 tcaagaaatc ctagaaaaac ttaattcaaa ctttacagag gaattaaata aaaaattatt
     3721 atcagtacac gaaaaaatag atgccttaaa tctttgggat ttagaaagtg aagctaagtc
     3781 agtacttaca aagcttggta taaatgactt taatcaaaaa atagcagaac tttctggtgg
     3841 gcaaagaaag agagtctctc tggcatcagc ccttattaca ccttgtgagt ttttgatact
     3901 agatgagcct acaaaccatc tagacaatga tactatagat tggcttgagg aatatcttaa
     3961 ttccagaaaa ggttcattgc ttatgataac tcatgataga tactttttag accgtgttac
     4021 aaatagaatt attgaactgg acaaaggtag attattcagt tatgatggga attattcaat
     4081 atttttggaa aagaaaatgg aaagactttc gctagaagca agtattgaag ataaaagaca
     4141 aaatttaata agaacagagc ttgcgtgggt aagacgagga gctcaggctc gtagcactaa
     4201 acaaaaagct cgtttacaaa gatttgatga acttataaat agagattctt ttaaacctga
     4261 tgaaaatctc gatatatctg ttgcatcaag tagacttgga aataaaataa tagaaataca
     4321 caatatatct aaatcatttg aagaaaatac tgttatagat aatttagaat acactttaac
     4381 tcgtacagat agaattggta taataggcaa aaatggtatg ggtaaatcta cccttataaa
     4441 tatacttaat ggagaaatag agcctgatag tggatatatt tcaattggag aaacagttaa
     4501 aataggttgt ttttctcaag acgactctca tatgaatatt gatatgaggg ctatagacta
     4561 tgtaaaagaa gctagtgact atatagaaac ttcagatggt actaggatta cagcttctca
     4621 aatgtgtgaa agattccttt ttaatggtac tatgcaatat actttgatag gtaaattatc
     4681 tggtggtgaa agaagaagac tacatcttct taaaatcctt atgacagctc ctaatgtgct
     4741 tcttttagat gaacctacaa atgatttaga tattgaaacg cttaaaatat tagaagaata
     4801 cctagacgaa tttaaaggtg tagtagttac agtatcacat gatagatatt tcttagatag
     4861 aatttgtaat aaaatatttg catatgaagg taatggaaaa attcatatct atacaggtaa
     4921 ttacagtgat tatcttattt atagagagat tcaaggaatt gaatttgaag aatctaaaag
     4981 ggaatcagtt aaaaaagatg actctactac cccaaaaaaa gaaaaaccta aaaatgataa
     5041 aaagcttaag ttctcatata atgaacaaag ggagtttgaa aatattgact ctgatataga
     5101 aaaactcgaa tctaaaattg caacacttga tgaaagtact gcaaaatttt caactgattt
     5161 tactaaactc caagaaattt tagatgagaa atctaaacta gaaaaagaac ttgaatacaa
     5221 atatgaaaga tgggaatatt taaataatct agctgatgaa atagctaata ataagtaata
     5281 ttaaaataat cttaagttta ataagttttg ctttaaatct gtaatttata aatattttaa
     5341 aggaagtgtc tcaaaatgaa ctttttagtt ttatgagaca ctctttttta tgagaaatca
     5401 aatatatttt catcatttca acaatacaaa agaggcttat ctctattttg agacagcctc
     5461 tttcataatt accttctaat ttatatgctc taatatatct ataataattc ttattatatc
     5521 tatatatcca gtctttcaat atagtgacaa ttaacttcaa tatgatttaa aaatactaac
     5581 aacctttcat aaagtggatg agattcttct ccatatttag cttctaggtt cttgcctatt
     5641 tctctaacag tcctttgacc atcaatttgt aaaaaaacaa aactggaata ttcatctagt
     5701 tctatttttt tgtatttagg tattctagct tttaactttc taaaaaactt ttgaatttta
     5761 tggtcctgtt tttcaagtat agtaacaaca cgattttcag ctacttcata ttcaagtcca
     5821 tcagtaattt taaatacaat atctaaaaca tcattactct tgctcattat tttttacttt
     5881 agataaaaca ataggtaatg ctgtaagcac aactaaaact actaataaag caattgccat
     5941 tgaatttgtt gcagcaaatc cacttggtac ctttggagtt acaactccag ttacttgtaa
     6001 tattatacct attaaaccaa tgatagaacc accagcaaca agaccagaag ataaacttat
     6061 accattagaa actttaactt ctttttcttt ttcagtttta gatactaatt caacaaataa
     6121 acgaattaat gctccaacta atattataga agtagttgca attggtaaat agaatcctat
     6181 agctatagtc attattggta aatttaatgc atatagaact attgccataa atactccaac
     6241 aataatcata acccaaggta aactacctga cataatacca gaagttaatg ttgacattaa
     6301 gtttgcttgt ggtaatgcaa attgtacatt atcaccagtt gctctaagtt ggtcagaaag
     6361 taataaaatt actcctacaa caactataac accaactata cttgcaattg taaagtaacg
     6421 ttgcatttcg tttttgctac caccaataat ataagttact ttttgagatt gagtataacc
     6481 tccagctata gcaatagcaa caactataaa tgaaccaaat aaaagtaatg atttattagc
     6541 ttctaaatca gtccatccca ttataacaaa cactaatgtt acaataacta gagaagctat
     6601 agtcatacca gaaactggaa gatttgatgt tccgattgtt cctgttaaac gaccagctac
     6661 tatgacaaat aataaagata ataaaagaga tatgatagct ccaataattg ccataacaat
     6721 gttaccagaa attaagaatg ctgctaaaaa tccaataact actccaccta ataaaagtat
     6781 catttgtata gatgaacctt cttctccttc tccagcactt gatttagctt taagtgtttc
     6841 ttttatagaa gcgattatag ttggtataag ttttattgca ccaataatac ctccacaaag
     6901 catcatacca gcacctatat attttacata actactagat attgctccaa catccatttg
     6961 gttaagaggc attgcaggat tattccatac catagctcca tctttagcca tatctgtaaa
     7021 gtatcctatt aaaggtgcta taccaaagtt tgataaaata cttcctgcaa acattgttaa
     7081 agaaacttca agccctacta taaatccaat tcctaaaagt agaggattta cttcaatatc
     7141 aaatttccat ttgtagaatt tgcttcctac aagactcatt acattgtttg ctacatttaa
     7201 gaatgaacca gttaatacag taataaatcc acttattaca aatccaattc ccatgtactt
     7261 aattgaatct ccaccagcct ctgaagcaac aagtgtctca gatatagcca ttgattctgg
     7321 gtacataagt ttaccatgtt cttcaactat tagatagttg tgaactatag cagcaattcc
     7381 tataccaaat aaaactccac caactccaac agctaagcct tctaagaatg taatttggct
     7441 tccaattaat ataactgctg gtaatacgaa aataaatcca cttgcaacag attctccacc
     7501 acttgacata ccttggatta agtttttacc aagaataccc tttgaacgag caaatgctcc
     7561 aacaaaagca gaacctatta tagctcctgg aatacctgcg gcaactgtaa gaccggcttt
     7621 cattccagag taagttgtag atgcggcaaa aatggcagct aaaataatac cgataatcag
     7681 tacagctaca tttccaccgg ttctggactt atctgtgata tatggaacgt aatcttttcc
     7741 actaacctca ccataagcac ccttaggtaa ttttttgttc ataaaatttt cacccctttt
     7801 caatttgtat atattataac atatatacat ataaaactta actttttatt attttacaaa
     7861 tagcgagtca aaactactaa aatttaaccc tatattatta atttctactt ttagatattt
     7921 ttatgctaat tttcttttta tttttcaatt ctactaaaaa ctattaagat ataaaataaa
     7981 aaaatttttt aatgtaaaat taattttgta aaaaaaatgc tttaaaacac ggaaaagtat
     8041 aaaaaataca ataatcattc ttataaataa attttcacca tagtaaatat atacagaaaa
     8101 ttcttgtttt tatgcaaatt gattatttta taaaaataag tgtcataaag aaatgtctga
     8161 aatcacttta tgacacctat cataataaaa aaattattct ggtaatgctc ttttatttac
     8221 ttttccgatt ggtgtaactg gtatcttatc tatcctcatt attattgttg gaatcatata
     8281 tggaggtaga tgctcattta aatattctct aatagttctt tcatttatct taccactatc
     8341 ttcctcatca attacataat atgcacatat ataaatatct ccatcatcat ttgttctcga
     8401 tatacaaaca acacttttaa tttctgtaat attatttatt atattttcaa tctcatatag
     8461 ctctatccta aactcatcaa tttttacttg attgtccatc cttccaatat aatctaaatt
     8521 tccatcatct aaccatctta ctaaatcacc agttctatac attaacttat tatgttcaca
     8581 agttttgtaa ggattctcta caaatacttg ctctgttagc ccttttttat ttaaatatcc
     8641 tcttgatatt tgaatgcctg atagacatag ctctcctggt gctccaattg gacatagttt
     8701 gttcttttta tctaatacat atgcataagt attaaatata ggttttccta ttggtatttt
     8761 tccatattct ctgtccacat gaaaagaagt cgatacaaca gtaaattcgg ttggtccata
     8821 ctcattatat agttcataat tacgctttct atatcctttt aatctttctc ctccagtctg
     8881 tacaactcta agtgaattat tattgcaatt ttgtataaat aaccttgcaa atgctgttgg
     8941 caatgctagt atagttatgt cattttcctc acagtattca tttactgtat attcatcaaa
     9001 tttagcttct tcaggcataa tatctattga tgcacctaca agcaaataag ggaatagttg
     9061 tttcattaca gcatcaaaac tgaaacttaa gtatatacca catatatcat tttctgtagc
     9121 gcttatagat tttacgctat aataacacat atttactaaa ctcttatgtt ctatagctat
     9181 ggtctttggc ctaccagttg accctgaagt agatatcgta tatgcacaat ttgatgatgt
     9241 tattttttca gttaattcat atccaaactt aaacatttct tcatcattta tatcgagtac
     9301 tatggtattc tcatttacaa ccttactttt gagttcatct gtagttatca ctactttcgc
     9361 attactatct tcaagaaggt aattcaatct ttcttctgga ttacttgtgt ctattggaaa
     9421 aaatgctccc cctgatttca taatccctat tgctgtaata attatgttaa tatctcttgg
     9481 taacattaca gctactatgt cttcactttc cactcctata tgatttaaat aacctgcaag
     9541 tttatcagtt acttcatcca attctttata tgttaacttt ctattttcat ccctagcagc
     9601 tatattacta ggtgttacct taacttgctc cctaaataaa tcaacataac ttttatcata
     9661 gggtacttca tattgattat tattaaagtc ttccaataat actttttctt cttccttaca
     9721 tacaaattct aaatcttcta ttaaaatatc attatcttct attatttgct ctattatata
     9781 atcaaattga tttaaaattc ttctaatagt ttctctttta tatatagatg gtttatactt
     9841 tatttttatt ttaagtgccc tcttagtata tactattaca gataaatcat atcctttttt
     9901 ttctatgtca taatcatatg ataaatcaaa acttttagct ataaatattg tgtttataag
     9961 cttagtattt agatgatttt tattctctat atctgataac aaatagtatc catactctaa
    10021 agacctgctc cactgccttt ttagcttcct tacaacatct ataaattttt cactttgtct
    10081 aactcttact cttattggaa ttatattcat tgttttacca aaaactacat catcttcata
    10141 tatatacttt tgaagtataa ggccatatat agtttctatg atagagtcta tgctaatatt
    10201 gttcacttta gaatattgaa ttaacctttc atgtttctca atattcatct taaaaacctc
    10261 atctactact tcatcagaac aatctttata aaaaaaatca tttggaattt ttgctatatt
    10321 cgtataatca tctaacaaat ccatccaata attcatagct cgttctaaat taacttcata
    10381 catatcaaaa ttccccctta ctactaagtc tatagaaaaa attcttttgg aattttattt
    10441 tcacaatata taaatatatt aataatacat atgcaagctc taaaaacatg agcttgcata
    10501 aaattacact tattatgact actaaattcg taatatttac aatgtttaaa atatattata
    10561 acacatatat gattttattt ttctacattt atataatata taaattacaa atagttattt
    10621 aactaatata taacaatacc ttttggaata tgatatatta tattaattaa atttttataa
    10681 gataagttat acttatagga gggaaccata taaatgaata tttatgctca tagggggttt
    10741 agtggaaagt atccagaaaa tactattcta gcttttaaaa aatgtttgga tatggatata
    10801 tatggaatag aacttgatgt ccacagaact aaggatggaa aaattgtagt tattcatgat
    10861 gaaaaggttg atagaacttt taatggacat ggttttgtaa aagactttac tttaagaaaa
    10921 ttaaaaactc taaactcatc ttttgaagga tatcaaagca ataaagaatg taaaattcca
    10981 actttagaag aagtattaat tctaatctca cctacagacc ttactttaaa tatcgaacta
    11041 aaaactgaca aaataaacta tcccaatata gaaaaaggcg ttttagagct tattttaaag
    11101 tataatatga aaaatagagt tcttatatct agtttcaaca gcaatagctt aaaaaacttt
    11161 cataaattag accctagtgt aaaaacagga ttattgtgtt atttacctat aaacaatgta
    11221 gtcaattttg ctaaatttct gggtaatagt tatcttcatc cacctctagt tttagtaaat
    11281 gaatctctaa ttgaactttg tcacaaaaat ctacttggtg tcaatgtata tactgtaaat
    11341 gaagaggatg atatactaca ctgtctaaaa ctcaatgtag atggtatttt tactaactat
    11401 ccagatattg cttcaaatct tttacactca aaacaataca gctaattata caatagtttg
    11461 tactccttga cttttatata attaatatgt acaattttat taaatttatc ttggaggtat
    11521 aactatggat aacttattaa atgaacttga agctttaaag agtaatttag ataatctacc
    11581 aatagaaaaa atagaaaatt atgatttgag taaaactgct ttatttataa ttgatgttaa
    11641 taatggcttt gcaagacaag gtgcattata ctctcctaga gtagagtctt taataaagcc
    11701 aattgaaatg tttacaaaaa aaatatcaaa caaattaaat agagtaatag catttactga
    11761 ttctcatact cctaaatcaa tagaactttt gagttatcct gttcactgtt tggaaaatga
    11821 tgtagaaagt gaattggttg atgaacttaa gtcgatagaa aacctacaaa ttcttcctaa
    11881 aaattctact aatggttttt ttgcattaga aaatttggat tttgataata tagataatat
    11941 aattatagtt ggcgattgca cagatatatg tatatatcaa tttgcaataa ctctaaaatc
    12001 atattttaat caacataata ttgaaaaaaa tatagttgta cctatgaatt tagtagatac
    12061 ttatgatatt cctaacgttc accctgctga gattttaaac ttagtatttt tcaacagtat
    12121 gattcaaaat ggagttaatg tattaaaaga gattcgttaa gcttctaata ctttacatcc
    12181 catataatac aattatcaga tatttatttt taataaaatt tctaatagaa aaacacagtt
    12241 ttagtgagaa gattacaatt taaaacacta tctattatta ctaatcttca caatataaaa
    12301 aatcgcagtt tagatattcc ttatacaaat ttataaggaa taataaattg cgatttttat
    12361 ttttaagtag tacaattaat tgatactact tcagtattta aattcatttt ttaataatca
    12421 tacttattcg tagtttcgca cattactacc tttatttttt tattaaacat attcttatat
    12481 tctaataaaa actcatttag ttctgaatcc ataagattta tttttctatc actacaaagc
    12541 attatctttt cttggtcgat tattacatta atatcattta agtcattttt tataaatgtt
    12601 gttatacaac tctttggttg gattttttct ccatatttct cataaaatcc tgcactcttc
    12661 ttaaatgact ctaaatcttc tgtagatact ttgctggaag tattattact cataaaacct
    12721 gccgtagaag ggtcgattcc aaagtttcta agttgttttt ctacttcttc agtgtccata
    12781 ccatatctct ccataaattg cttttgtatt ttcataaatt tttcttgaga tatgttgtta
    12841 tctctcatat aatcgaatat ttttcttgaa aactcagcca tagacatctt tccatccata
    12901 acagaaaaat ataagttata tatgtattgc tcaaatttat ctacatcaga atctttaact
    12961 ttgttcttta gttcgttaaa gtctattatt ctatcatcag acatttttca tcctcctagc
    13021 taaattttta cttgtataag attataccat atttctatta tatttatata cacactattt
    13081 ttttaattta acgatttttt ctgtagcatc taattcgcac attctgccca aaggaatagt
    13141 tatcattggg atacaatgtc ctgactgaag attatatata gaaggtttat tgaccttatt
    13201 tgcaaattct attagtaatt ccatcaaatc attttcttca atacaatcct taaaatctcc
    13261 aaaaattata ccattacatt cctcaagttt tcctgaagaa tatagttgtg ttaacattct
    13321 atccagttta tattgaggtt ctccaatttc ctcaataaac aaaattttat cttttgcatc
    13381 tatttcatat tttgttccca ttgtggcaat tattaaggat aaatttcctc ccattattat
    13441 tccctctgct ttaccttcac atacagtata aatcttttct tcttttggat tttctaaata
    13501 taattcatct ttaaattcta aagcatttac caatgattta taggtaaatt tatcccattt
    13561 acctacattt gctgccatta ttccatgata tggtaaaaga ccacaatttt tatagaaagc
    13621 taaatttaaa cctgttatat cagaaaaacc tataaatggt tttggatttt gctttattat
    13681 atcataattt attttatcca atattcttgt tgttccataa cctcctctta agcacataat
    13741 tatatctaca tctttatcta aaaacatatc ttctatatcc cttgctctaa tatcatcatt
    13801 accagctaaa taacctttat aatttaaact acaactttca gaaaacttaa cttcataacc
    13861 ataactttct aaattaaact ttatttcctc caaacttgta tttcttaatg gcccagatgg
    13921 cgcaattatt cctaacattt aaacacctct tcttataaaa tgatatattc ttctccatac
    13981 taaatatctc taatataatt ataagatatt ttatttgtac ttgatacata ataatgattt
    14041 acataaaata atataattaa tgaagaaaaa taaattttta atatagttag gatgtgtttt
    14101 atgcttaata taaaaactct ttgtcatgag ataaatgatt gggttataaa tataagaaga
    14161 gatttacata agactccaga acttggatta gaagaatttc aaactaagaa aaaaattatt
    14221 aaatacttaa atgaaatagg tataaattat attgaatata agaatcatac agggataact
    14281 gcatacatta acgtgagtcc aaactttgaa acagttgcta taagagctga tatagatgcc
    14341 ttacctataa cagaagaact taattattct tataaatcta ttaatattgg caaaatgcat
    14401 gcatgtggtc atgatgcaca cactgctatt ttactgggta cttgtaatat actatttaaa
    14461 ttaaaggatt acttaaatgt aaatgtaaaa ttcttttttc aacctgccga ggaaactatt
    14521 ggtggggctc aacttatgat agaagatgga tgtcttgaaa acccaaatgt aaaatatata
    14581 tttggacttc atgtaaattc caatatcaac aaaaatctaa ttgaattaaa atacaacact
    14641 ctaaatgctt ccactgatac acttcaatta actgtacatg gttcaaaatg tcatggagct
    14701 tatccacatc aaggtattga tgctattgtt atatctgctc atattattac agctctacag
    14761 acaatcgtta gtcgaaacac aaatccaact gattctgtgg ttatttctct aggaaagatt
    14821 gaaggtggaa tcaaggaaaa tatagtttgt gataaagtgg ttataagggg tactttgaga
    14881 accttgactc ctgaaactag agagttttca aaaaaacgaa ttagagaaat ttgcgatttt
    14941 acttgtaaaa catttggagg aagtatttct gttgaaattg aggaaggtta tccagctctt
    15001 atcaattcaa accatctagt agattacgtc aaacaaaatg ctgtagaatt gtttggagaa
    15061 gaaaatatta tccttaaaga ctcgcccact cttggggctg aagacttttc gtattttcta
    15121 agacactgtg aaggagcttt ttaccacctt ggttgtgcaa atcgtgaaaa aaacattact
    15181 tcccctttgc atacctctac ttttgatatt gacgaagatt gccttattac tggtgtcatg
    15241 ctccatgtaa aaaatgtatt atcattctaa ctattaatat ttttatttat tattttatat
    15301 tgaccctcac atgatgtcat gttataccct aaaattacaa atgataaatt aaataattgg
    15361 aggtaatatt atgaaagaaa tattaataaa tcctataggt aaaatatgta ttcaaggcga
    15421 agaggtcttt attaaattag acaaaaagta tgtacctgca ttaaatgaac tcgacgattt
    15481 cagtcattta aatgtatttt ggtgggctga tgggtttgat aatcctgaac taagaagtat
    15541 tttagaaacc ccaaaacctt ataaaagtgg accagacgtt ataggaatat ttgcaacaag
    15601 gtcacctata agacccaatc caatagcatt gactgctgta caaattatca atatcgacca
    15661 tgaaaatggt ataataaaaa taccatatat tgatgctaat gataatagtc ctgttataga
    15721 cttaaagcca tatactccaa gcgttgatag ggttgaaaat ccgtccattc ctaaatggtg
    15781 tagtcactgg cctaagagtg tagaaaaatc aggagaattt aattgggaag atgaaattga
    15841 attttaaatt acttttttct tgatttcatt aacttgtttt ccacatttat tttatttaca
    15901 cttaccaaga ttttatgtca catata
//
LOCUS       DAELTA010000092        15759 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.092, whole genome shotgun sequence.
ACCESSION   DAELTA010000092 DAELTA010000000
VERSION     DAELTA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.092"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            83..784
                     /locus_tag="KPG13_002394"
     CDS             83..784
                     /locus_tag="KPG13_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG0986104.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1055..1873
                     /gene="larE"
                     /locus_tag="KPG13_002395"
     CDS             1055..1873
                     /gene="larE"
                     /locus_tag="KPG13_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG0986105.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1928..3076
                     /locus_tag="KPG13_002396"
     CDS             1928..3076
                     /locus_tag="KPG13_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG0986106.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3212..4600
                     /locus_tag="KPG13_002397"
     CDS             3212..4600
                     /locus_tag="KPG13_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0986107.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4629..5327
                     /locus_tag="KPG13_002398"
     CDS             4629..5327
                     /locus_tag="KPG13_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986108.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5330..6763
                     /locus_tag="KPG13_002399"
     CDS             5330..6763
                     /locus_tag="KPG13_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986109.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6785..7522
                     /gene="truA"
                     /locus_tag="KPG13_002400"
     CDS             6785..7522
                     /gene="truA"
                     /locus_tag="KPG13_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG0986110.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7633..7840
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8093..9292
                     /locus_tag="KPG13_002401"
     CDS             8093..9292
                     /locus_tag="KPG13_002401"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG0986111.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9539..10585
                     /locus_tag="KPG13_002402"
     CDS             9539..10585
                     /locus_tag="KPG13_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG0986112.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10693..11856
                     /locus_tag="KPG13_002403"
     CDS             10693..11856
                     /locus_tag="KPG13_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0986113.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(11945..12325)
                     /locus_tag="KPG13_002404"
     CDS             complement(11945..12325)
                     /locus_tag="KPG13_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG0986114.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12328..12987)
                     /locus_tag="KPG13_002405"
     CDS             complement(12328..12987)
                     /locus_tag="KPG13_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG0986115.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13093..14067)
                     /locus_tag="KPG13_002406"
     CDS             complement(13093..14067)
                     /locus_tag="KPG13_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0986116.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14107..14454)
                     /locus_tag="KPG13_002407"
     CDS             complement(14107..14454)
                     /locus_tag="KPG13_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986117.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(14672..15628)
                     /locus_tag="KPG13_002408"
     CDS             complement(14672..15628)
                     /locus_tag="KPG13_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG0986118.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT         6174 a         1681 c         2567 g         5337 t
ORIGIN      
        1 tttaaataat agtggtatta aaaatctgac aacaaaaata aaactttcaa aaaattaaat
       61 ctaaaaagaa taggagataa atatgagtat tgaagattta tatgattttg aatgtgttcc
      121 tttgaacaat gagattgata ttgatgtttt atttgaaaag atgatagaag atggaaaaga
      181 aaaagaatat acaccaatta tagtcattga tgaaaaagta gggctattaa gaaaaaatat
      241 agatatgatt actaaagagt atggttctct tgaaaactat agagagtatt gtttaacaaa
      301 atataatgaa attgatgtaa atcaattttt tgattttagg aagagtgaga tttcagaatt
      361 attaaaggag ctaattgatt tagaagatga tttttatgat tataaacatt ttagaccaat
      421 tatgaatata gatatatttc atgaatataa caaagtttac atagctaaaa ttccaacaat
      481 taaaccatat gaagtatttg catacatacc tattggcgga tttaatgatt gtcctagaga
      541 tgaagaacat atagctattg caaaatattg gtatgaaaag catggaatct ttccaatcgc
      601 aattggatgt gatacagttc aatattctgt aaaaaattta actaaagatg gtaaaaaatt
      661 tgatgaatta tgtattgaat tagtatttta ttgtgaggat attattatac aagggtatga
      721 aaccttaaag gctcttaagg atgtattaaa acgctctaca atagtgcttt tttggtggga
      781 ttgatattcc atcatttaga tagtattaaa ttgttagtca ccaagaatcc acttgcttta
      841 gttatgggga gtgtgtcaaa ctaggttttt atacacagtg tagcatagta atatgtttaa
      901 attacaaagg ttaaaaaaat actttaatat ttttaatatt ataattaata gatttaaagg
      961 taattataat ttataaaata aatactttat atgttataat cattaagaat gtaaaaaaat
     1021 aatatatagt tttatagttc atatttttcc agatatggag gtaaattttg tgaaggaaaa
     1081 agaaaaatta gataaattaa aaaaaatgtt acttgaatta ggcagtgttg tagttgctta
     1141 ttctggtgga gtagatagta attttttgct taaggtcgca aaagatacat taggtgaaaa
     1201 tgttatagct gtgactattc atgcaatgat gcattcaagt agggaaattg aagaggctaa
     1261 acaatatact caaaattttg gtgttaaaca tatcatatta aatatagaaa actttgattt
     1321 aaaagaattt aaagaaaatg gtgttgatag atgttatcat tgtaaaaaat atatattctc
     1381 taaaataaaa gaggtagcaa aagaacataa tataaagtac atagtagatg gaacaaatat
     1441 agatgattta ggagactata gacctgggct taaggcattg agtgaattag gagtaattag
     1501 tccattaaaa gatagtagtt taaaaaaaga agaaattagg tcgctttcaa aaacattagg
     1561 attaaatact tttaataaac catcatttgc atgtcttgcg agtaggatac cttatggagt
     1621 tgaaataaca gatgaaaatt taagaatagt agaaaagagt gaagaatatc tatccaattt
     1681 aggtttttct caatttagag tgagaatgca tggagatata gctagaatag aagttggaca
     1741 ggaagaacta ggtaaatttt ttgaaaataa caattttaat aaagttgata ctaaattaaa
     1801 aatatttggc tttaaatatg ttacactaga tatgtcagga tataaaatgg gaagtatgaa
     1861 tttaaatgtg tagattattt taaaaataaa taagttttat agttaatata aggaaacagg
     1921 aggttttatg aaaaattata cgttaatatc gccatgtttt tttggaatgg aaaaaatgct
     1981 ggctagagag attactaatt taggatatga aataataaaa acagaagatg gaagaataac
     2041 atataaaact gatgagtttg gaatagcaaa gtcaaacatg tggttgagat gtgctgaaag
     2101 agtacatctt aaaattgctg aatttgaggc aaaaagcttt gatgaattat ttgaaaatac
     2161 aaaaagaata aactggtcta gatatatacc ttatggagct caatttccta tatcaaaagc
     2221 ttcctctata aaatcaaaat tatacagtac accagatgta caagccatag taaaaaaggc
     2281 tatagtagaa agcttaaaga aaagttattt ggaggatggt ttgcttaaag aagataagga
     2341 gaaataccct atttttgtat ttatacacaa agataaagta accatttcta tagatacaac
     2401 aggagatgct ttgcataaga gaggatatag agaaaaggca aataaagctc caataagaga
     2461 gactttagct gctgggctta tttatttaac tccctggaaa gcaggtagag ttttagtaga
     2521 ccctatgtgt ggttctggta ctatattgat tgaagctgct atgattggta taaatatggc
     2581 acctggctta aatagagagt ttatatcaga aaagtggaga actcttgata agaaaatatg
     2641 gtgggatgta agaaaagatg cttttaataa gatagataat gaatctaaat ttaagattta
     2701 tggatatgat atagatgaag aatctataga tatagctaga gaaaatgctg aaatagcagg
     2761 tgtagatgaa tatatagagt ttaatgtagg agatgcaact caatttaaaa gtgaagatga
     2821 gtttggattt ataatcacta atcctcctta tggagaaaga ttagaagata aagacagtgt
     2881 taaacagttg tataaggagc ttggttatgc atttagaaaa ttaaaaaatt ggtcgtatta
     2941 tttgataact tcttatgaag actttgaata tgagtttggg caaaaagcag ataaaaaaag
     3001 aaaattgtat aatggtatgt taaaaactaa ctttttccaa tatccgggtc ctaagcctcc
     3061 tagaaataat aaataagttt tacatgattt atttgtaaaa tataaaattt tattaaaata
     3121 agtaaaacat tatgatgcct tgaaacgtct aattattgtg aggagatttt tgtgaacaaa
     3181 tttaaagatt tataggatta ggaggagtta aatgaaagtg ttaaagctta ttactgtagt
     3241 agccctgatg atgttatttg ttactggatg taatataaaa actgaatctc cagaacaatt
     3301 agcgaagata cctgactatg atgatacaaa aaaagtattg tatgatggta ttgaccagtt
     3361 actaaaacca gattcatcta tgatattgcc ttctaataca aaagaagtag gaaaaataaa
     3421 taaggtagat ttaaacagcg atggaattga tgaattagta gtatttgaac aaaaagaaga
     3481 tttaagtaat aatgtgagtc aggtagggtt tgtaaccttg agttacaatg gagaaaaata
     3541 cgtacttgga gaccattttt tagaaaatgg cgaatcaata gaatatgcta atttttatga
     3601 tttggactca gatggatata aggaagttat cttattggtt aaatccaaag acaaaacgaa
     3661 tatgcatata tataaagtag tagataatga aataacaaaa atatatgact tggatgcatc
     3721 atggcttcaa aataaagaag attttaatga tatgaaagta aaaattggat atatagatgg
     3781 agatgacaag ttagacatat tgatgcttca tttgaataat aagactaatg aaatgtttgc
     3841 aagtgttgca aattttgatg ggaaaatgaa aatcaaagat tctgttaagt ttgaaaatgt
     3901 aaaaaactta agtgaattat atataacatt aggtaatgtt gcttcatctg ttggaaattc
     3961 ttctgcagct ataaaaggta ttgtacttga tattcctata ttgaaagata ataactatat
     4021 tacgcagata ctttatatga aagatgggaa aattaacaaa gcttttagtg attatgataa
     4081 aactattact aaatcttatt atatacctgt agatgatatt gacaaagata agattataga
     4141 aatacctata gttgcaggca gtacaggtaa taataaaaat acttatagtt ctaaatcatc
     4201 tgcaactata agttggtata gatggaatgg aaaagaaggt gctgactcaa gtttaatatt
     4261 tacaagtcag atatattata attataaata taattttaaa ttatttattc caaataattt
     4321 atttgataaa atacttgttg aacaggaatt tgttggagaa aaagcagtat ttaaatttta
     4381 ctattttgac tattcagacc gtaagaattt atttaatata gtagtagaga gtaaaaataa
     4441 acttgaagat agaaaaaata caggtactca aaattctatt gtacttcaag agagcgatga
     4501 atatactttc ttactagttg taaacaatgc aaatgaaatg gataagctag atatgactat
     4561 agacgcatta aaagaatatt tctcattaat atatggttaa tattatactc aattggaggt
     4621 tttattttat gaaggaaaag atacttatac tagaagatga aattggtatt agaagttttg
     4681 ttagtataaa cttaaaaagg gaaggctatg aaatagttga ggcaggtact ggaagagaag
     4741 ctatagaaaa aatgacaaca gaaaaggata ttacaatagc tcttttagat gtaatgcttc
     4801 cagatataag tggtatagaa gtatgtaaat ttataaggga gaattttgac caagtaggta
     4861 taataatgct tactgctaaa gctcaagaag atgataaaat agaaggattt atctcaggag
     4921 cagatgatta catcataaaa ccatttagta taaaagagct tttagtcaga gtttctgctc
     4981 ttcttagaag agtagcaaag gatgattcaa gtgttaagtc gagtgagata gtttctccac
     5041 catttatact tgatatagac aaaagaaaat tatttaaaaa tggaaaggaa attgagctta
     5101 caccaactga attttcaata gtgaaatatc taatatcaaa tgctaagcag tcgttaagta
     5161 gagaccagat attagacgaa gtatggggaa ctaactattt atatgatttt aaaatagtag
     5221 atgtaaatat aagaaggata agaaataaaa tagaagatga cccatcaaaa ccaaaatata
     5281 tacaaactat ttggggttat ggatactgtt ttagaaagga agaataatag tgttacattt
     5341 tgaaggtctg agaaaaaaag taataaaaaa ctattttata ataattatta taatggttac
     5401 tctgtttgaa ggattgttca tgttttacat acaaaactac tattatgatt ctgtcaaaca
     5461 gctacttgaa tctgaaataa aatatgctga tgaatataat gctattacaa tggaaactac
     5521 aagttttgag aaaaaagtaa aaaatatatt tgataaacaa cccttaacta aaaattcaga
     5581 atttggaatt tcaataatag ataaagataa aaatattata ttagaccaat atggttttaa
     5641 aagtaaagaa aaagcaaact atgaagatgt taacaatgca ttaaaagata taaaaactaa
     5701 aaatttaaca ccttatactt atagaatacc agatacaggg gagcatgtta tgagtatctc
     5761 cttaccactt aaggtaaata atataataga aggtgttgtt agatatacag tatcattaga
     5821 tgctattgat aatgcaatat taaagcaagc tacatggctt atattagcag gaatatttat
     5881 acttattata gctatactaa taagtcttaa atttgctgag actcttataa aaccattacg
     5941 agagctaaag aaatttgcaa atgaattggc tgtaggaaat tataatataa aattagaaaa
     6001 gatgaagatt gtagatgatg aaattggtga tttagcacag acatttgaac acatggcaca
     6061 tgagattgac aaaagtgaaa aattaaaaga ggaatttata tcatctgtat ctcatgaact
     6121 aaggactcct cttacatcta taaaggggtg gagtgaaacc ttaggttatg aaagtataac
     6181 aagagaagaa ctagacttag gtttaggcat tatacaagat gaaacagaaa gacttataaa
     6241 actagttgaa gaattattag acttttcaag gctttcttca gacaggataa aacttcatgt
     6301 tgatatagtt gatgttgaag gtttaattgt aggtgttgta aatcaactta aagttaaagc
     6361 agctgaaaaa gatatatcac ttttatttga atttgaaaat gaatttatag agaacattca
     6421 aggagataaa aatagattaa gacaggttct cataaattta atacaaaatt catttaaatt
     6481 tacgagtcaa ggtggttaca taaaagttgt agcttctcaa gatgaagaga taacaaccat
     6541 atcagtagag gataatggtt caggtataga aaagcaaaat ttaaataaag tgttagataa
     6601 atttttccaa gaagattata ataaggctgg tagtggatta ggactagcaa ttagtaatga
     6661 aattgtaaaa cttcatggtg gaagaatgaa aatagaaagt gaaaagaatg ttggaacaaa
     6721 aataactttt aatataaaaa ataaatttgc taaacaagca taaattctta gaaagaggat
     6781 taagatgaga aacataaaaa tgatagtagc atacgatggt tcaagatata aaggatatca
     6841 aaaattaggt gacaataata tgactattca agaaaaactt gaaaatgttt taagtaaaat
     6901 gactaatgaa acagtggaga taataggttc tggaagaact gatatgggag ctcatgcaag
     6961 aggacaagtg gtaaatttta gaactaattg tatggattct ttggataaaa ttcaaaaata
     7021 tttatatgaa tatttaccag aagatatagt tgtaaaaact gttgaagaag ttgatgaaag
     7081 atttcattca agatacaatg taaaatcaaa aacttatatg tataaaattg acaataataa
     7141 gtatcatagt ccttttataa gaaaatatgc tactcatgta agtaaaaaat tagatttaga
     7201 tagaatgaga aaagcaagtg agtatttagt aggagaacat gattttacca gttttgcttc
     7261 ttctaaatct aagaaaaaat caaatgtaag agaaatttat tctataaata taaaagaaga
     7321 tgataatgtt atagaaatat atgtagaagg aaatggattt ttgtataaca tggtaaggat
     7381 aatagtaggt gctttaatag atgttggtct aaaaagaaga gctccacaag atattaagta
     7441 tatgcttgaa tcaaaagata gatgtcaatc atcagatact gcaccagcta aaggactatg
     7501 tctttggaaa gtaacatatt aatcaattat ataaaactct ctttaaaata aaaataaaat
     7561 aaattatata gttgacataa atggaaaaat aaactattat atattttata agagagatat
     7621 aaaactttat atttaaatcc tgtgagaaag agagtaactt ctatgtgttt tatagagagc
     7681 tgaggatggt ggaagcttgg caaataagta ttagtgaaaa gaactttgga ggatactaat
     7741 tgaatttagt atgttagtag gtaatctcgc cttaagagaa agagtggata agtttatctt
     7801 atcaattaga gtggtaacgc ggatatattt cgtctcttgg tttttccaag aggcgttttt
     7861 taatatattt aattattagt gtttatctgg tatgttgaac atttactaat tgattagcta
     7921 agtttatcaa taagagtggt accgcggata tttcgcctct tagtatttta aaatactaag
     7981 gggttttttt agtattaaaa tgattttatg tgagtctaaa ttctcagagt cattcaatat
     8041 taagaatttc tataaaataa aaattataat ttaagattgg gaggaattaa aaatgaagga
     8101 aaaagtagta ttagcatatt caggaggact tgacacatca atcataatac cttggttaaa
     8161 ggaaaattat gaagatatag atgttatagc tgtttgtgga aatgtaggtc aggaagataa
     8221 aatggaagat gtatatgaaa aagctttaca aagtggagca tcaaaggcat atgtggatga
     8281 tataagtgaa gaatttgtaa ctgaaactat atttaaagcc gtaaaagcag aggctaaata
     8341 tgaaggaaaa tacttattag gaacttcttt agcaagacct ataatagcta aaaaattagt
     8401 tgaagttgct cataaagaag gtgcaaaata tatatgccat ggatgtacag gaaaaggtaa
     8461 tgaccaagtt agatttgaag ctacaattgc agctcttgac ccgactataa aagttattgc
     8521 accttggaga atttgggata taaaatcaag agaagatgct atagactatg ctgaaaaaca
     8581 taatataaag gtaacagcaa ctaaagctaa aatatattca gtagatgcta acctttggca
     8641 tgtttcaaca gaaggtggag atatagagca cctagaaaat gaacataaaa aagatgttta
     8701 taaacaatgt gtagacccag aagatgcatg tgatgtagct gaatatgtag aagtttactt
     8761 tgaaaaaggt gtacctaaaa aggtaaatgg tgaagaatta tctccagttg cactgattca
     8821 taagttaaat gagttgggtt gtaaacatgg aattggtgtt atagatatag ttgagaatag
     8881 acttgttggt atgaaatcaa gaggtatata tgagactcca ggaggaacaa tcctttatga
     8941 agcacataat attttagaaa gtgctacttt agacaaagat acattacatt tcaaacaaat
     9001 ggtatcttat aaatacggag aacttattta caatggacta tggtattgta aattaagaga
     9061 atctatagat gcatttatgg aacaaactca agataatgtt acaggaacag taaaagttaa
     9121 actttacaaa ggaaatataa aacctgctgg aatatttact gaaaatgctc tttatgatga
     9181 aggaatatct tcttttggaa atagtgaact ttatgaccac aaagatgcag aaggatttat
     9241 taatttattc actttaccat taaaaatcag agctatgaaa gctggaaaat aataaaataa
     9301 attatttaag agtgtataaa aactattttt aggtttttat atgctctttt tttatttgaa
     9361 tttattaact tatacagtaa atagtataaa aaaatatatt atagttccaa atggcttaat
     9421 aatgtgaaaa tataagacct aaaatataaa ttttataaat acaatagtta ttgactctct
     9481 cattgtaaga gggtttatac tgaatctaat gaaaatttat agtgaggaga tagattatat
     9541 gagaaaaata attgaaatta aacatcattc acatgattat gagtgtatgt ggaatggtat
     9601 agaagattta tatataaata aaaccggtga atgccttcca aacgattttt tctttttact
     9661 gtcaggtttt ggttcatttt gttatatgaa aacaaataaa gctgatttga aaagaatggt
     9721 agctttggga gatggaagga caaaacagat gtataaattt ttagcaccaa ttgttggatt
     9781 tgaatacaaa catcatgagt ttaaaaagta tgagcaagca ttaaagaaag caaaatctga
     9841 aattgatgat ggatatccag ttattttagg agctttagat atgtattatc taccatatta
     9901 taagaagcta tatcataaag aacatattcc ttttcattat atattaatgg taggatatga
     9961 tgatgaagga atatatctgt atgattgtgg tagaacagaa ttattacatc tttcttatga
    10021 taaattacgt gaaagcatga attgcaattc ccctggatta agcaaagaaa atactatttg
    10081 cacaataaga atgaatactt caaagaataa atataacatt gctagagaag ctattgctat
    10141 aaagcgtgat atgtttttaa atccacctaa aggatttttg ggatataaag gttttgaaaa
    10201 gtttattaaa gagttgccac agtggaacac tgaattggat aaagatgatt atgataagat
    10261 tttgaccaat atggttatgt tttttggaac agtaccaaca attccaaatg caatcaaagg
    10321 aattaaagaa ccagatactg ttcaatttaa aggtggattt gataaaatga gtagaatgct
    10381 caacagtatt ggggaggaat acaaaaacga ctcttttata caagtagctg atatttttga
    10441 taaaactgct attataattg agaaaatatc caatattata atagattact taactcaaac
    10501 atgtgatgat acaggacaac ttccgttatt gttctctgag gtcttagagc atatgaaaac
    10561 aggatattta atcttggatg tataaaaaat atattgttga ccctaacatt acgtaatagt
    10621 gtaatatgaa aatatagctt gaatatatta gtaggtattg atggctgtaa aattaaaatt
    10681 tgaggtgtgg atatgagaac agtgaaacaa gtttctgatt taactggaat aagtgtgagg
    10741 gcattacatt attatgatga aataggtctt ttaaaaccaa ataaaattac agatgcagga
    10801 tatagacttt atgatgatga atctattaaa actcttcaac aaattttatt ttttaaggag
    10861 attgatattc ctttaagaga ggttaaggaa ataatgtcaa gccaatattt tgataaggtt
    10921 gaagctctaa aaaatcaaaa aaaattactt attttaaaac gaaagagact ggatgaattg
    10981 atagagctta taaatcaaac tctaagagga gaaggaaata ttgattttaa agagtttgat
    11041 atgagcgagt attttgatgt tttagaagaa tttaaaagag agcataggaa taaggtaatt
    11101 aaaatttatg gtagtgtaga aaaatacaat gaatatatag aaagagttaa gtctaatgaa
    11161 gagaaaattg ctaaaatggc tataaaacaa tatggaacta ttgagaagtt tgctaaagct
    11221 ataaaaacaa attttagcag tgatatattg aatttaggag aaaaatttga tagatataaa
    11281 aatgattgtt taaaagaaaa acatccaaaa ttaaaagaat tatatagaaa gcttgtagaa
    11341 gatttaaata ggaatccttc ttcaacagac cttcaagaaa tagcaaaaga gataacagat
    11401 atatctaaaa aagattatga aattttctct atggatactg gtgatgacaa ctggtattat
    11461 atggttcaaa attatctagt aaatcctatg tggatagaag aagtagataa aaagtatgga
    11521 agtggagcag gtaagtttat tggacaagta ctaaagactt atctaagaga tagaaaacca
    11581 aagataaata cattgtatga aaaacttgta gaagatttga gtagagattg ttcttcaagg
    11641 gaagttcaaa gcattgttga agaaatagac aatgaaatga aaagaagtaa tgaattttat
    11701 aaaatagaca atggtgaaag atactttgat tatatgtcag agctttattt acaagattct
    11761 aactatataa aagtaactga taagaattat ggtgatggtg catctaagtt tattggagaa
    11821 gcttttaaga tttattttga taataataat tgttgaaaat ttggaagtaa aaaatatgca
    11881 agttttataa aagcacaaaa ataaggatgt agattagctc catctatatc cttatttaaa
    11941 gttattaaaa tatatagttt acatcaaact taacactttc aataatattt cccaaatatg
    12001 tttttatact ttcaacttct tctaaagatt ttaatttatt ttcagtataa attgctttgt
    12061 ccagaatatt aaaccttaat cctatttcat caaagtactc atgatttaaa actttgtacc
    12121 aatggttttt ttctttattc caactcgtag atattgaatc agcttctttt tgagcttttt
    12181 cccaatcttc attttctata gatttttcta aaacagatat attgtcttta tatccttctg
    12241 taaaatcata tagtttattg ttaacataaa atccaaataa tacaaataag atagtccata
    12301 gtataacaaa tgttgtagat ttcaatatta atcacccttt ttattttgaa taaatatttt
    12361 gtcattttca tctaaaacac aaataagaac atctttaaaa gtttctatgt gattagattt
    12421 taaaataccc attaaccagt tagtatcttt ttgagctatg tctagatttt tatttattat
    12481 tctaccatcc agtataagtg ggataggtaa atgattaaaa gctcttggag gagtactatt
    12541 gtagcttgat gctggtacaa cacttagatt accatcagtc tccaaaatag catattgtac
    12601 atcttttagg ttaaagtatc cttgaatcct aagttgttct aataattcat ctatactaat
    12661 tctttctttt ttaagctctt tatatacaat ttttccttta tctattaata cagaaggttt
    12721 acctgataaa aatgaactta attttctact ttttaatgat aagaatgaaa ttagagtctg
    12781 catgattaca agtccagata ttgctgcaac accatttatg attggtatat tattattttc
    12841 cattgggaca gatgcaactt ctgcaattaa tagtgttata actaaatcaa aacagttcat
    12901 ttcagctatt tcaccttttc ccatgactct aagtgcaatt agcactgtta tgtataatat
    12961 aatacttctt attaatacta ctgtcatatc tatcaattac tcctttaaaa aatatttaca
    13021 cttagttagt atatcctata taaaaaattt aattcaaagt ataaaaaacc ttaaataatt
    13081 ggattcaact atttaaggtt tttttgataa ttttaatatt aaatttttta tggattttct
    13141 gttactttta tttattttaa taacagattc aacaaaactc tttttatctc ctaattttaa
    13201 gataaaatca ggatattctt caaattcatt gagttttatt aatttgaaag aagaagatag
    13261 atttttaaag ttaataattt tagagttatt cactaataca cttctttttt taaaataaat
    13321 ttctttttta cttatattta gtatagttaa tatttgttta agatatatta ttatatcttc
    13381 tggagttcta tcaaatttga gttcaaaatt ttcatcttca aagtagggaa tataatataa
    13441 aggtgttttg agagcaggta atttattata taattcttca agaatatcga tatttttagg
    13501 ggagttaaag aaaaagcatt tacatacatt cattaaatcc acagcatctg aaagtgagtt
    13561 atgtttaata tctccttcta cagaaaaaat ttttttcata ttcagtaaag atatttgctt
    13621 agataagatt ctattattgt atgtaatatg ttgagatata aaaggttgaa tgtcaatcat
    13681 catattaaat aactctaagc atttacctgt atatttatat actctaaatg aagttagaag
    13741 gccagttata tcaaaatcac cccaattata tattgtaaca tcatcaaatt tacaaaacca
    13801 tttataaaaa tcatccataa catataaaag attaggagca ttattgatat ctagttggtt
    13861 caatttggtt aaatttttac atttatttga aagtatttta ttgtattttg gcttaactaa
    13921 agagtaataa ttatctattg tattaaattc gttatcaact aaaactccac caatagaaat
    13981 taattccgca ccattatatt cttttggttt atttttatta tcatctagta tattaaattc
    14041 aaagtctata aataacttgt atttcataaa tctaaagcac atcctcaggt aaattattta
    14101 ttgagcctaa gcatcataag aatcaataga tataatgact ctaaacatca aaggttcatt
    14161 acaactttta tttatttcat aatttaattc aaataaatct tttaagatat aatttataag
    14221 tattctttcc caacccattt ttaaattaac tttagagtct attaatatat tttctatctt
    14281 tttaaatatt tctatatttg ctggtattat actgagctca atattacttt taaacgggtt
    14341 agactgtaaa tctgttataa gtatattttt taattcctct gtaagttcag aaattaaact
    14401 atcacctatg tattttttta tgatttcctc tctaatttct tttaaattta gcaatattag
    14461 tacctgctac ttacatagta gcttttcctt tccttttaat actattattc atttttgaat
    14521 cttatttaga tatattttaa gattaaaagt ctttagctat ataatttttt acccattttt
    14581 aacttatatt aacatattac aggcttatat ctccaccctg tttacattga ggatggagta
    14641 tattgcctat tttgataaaa atgataggtt gctatttgcc tattcctttt tctgataatt
    14701 tttctaacat atctttggtc attttatcta aatcataact aaacttaaag ttccattctt
    14761 ttactgcact actactgtct agagagttag gccaagaatc agctatactt tgtcttacag
    14821 ggtctacatc atattctatc acaaaatcag gaatatattt ttttatagaa tcagctattt
    14881 cctcaggaga aaagctcata gcagtaatat taaaagcatt tctatggatg agtttatcag
    14941 ctggagcttc gattaaatcc actattgatt gaagtgcatc aggcatatac atcatatcca
    15001 tttttgtacc ttctgctatg taagatttat atctcttatt ttttaatgcc tcataatata
    15061 tatcaactgc ataatctgta gttcctccac caggaggcgt aacataagaa ataagacctg
    15121 gaaatctaac acctctagtg tctactccaa attttgagtg ataataatca catagtaatt
    15181 ctccagaaac tttagtcaca ccatacatag tattaggtct ttgtaatgta tcttgaggag
    15241 tcatattttt tggagaattt tcaccaaaag cagcaattga actaggtgta aaaaatttac
    15301 aatccaattc tttggctatt tcaagaccat ttattaatcc attcatattt aagttccaag
    15361 cttctagagg tttactttca gcaactgcag aaagtaaaga tgctagatga attatagtat
    15421 ctacatcata tttttttgca atttcaaaaa aacgattctt atctgtaaca tctaatatct
    15481 caaatatacc agattcacat actggattac cttcttttac tctccttgaa ctagcgatta
    15541 cattttgttc tccatactca tttctaagct ttattgttaa ttctgaacct atttgtccta
    15601 atgcacctgt tataagtatt tttttcaatt ttacaacctc ctaagggttt aatttatgta
    15661 gtactactta tttttatttt agcaaatctt ttgtttaaat acaaagaatt aaagataata
    15721 atatacgact taattgaatg aagtgcagta agtgcctaa
//
LOCUS       DAELTA010000093        15667 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.093, whole genome shotgun sequence.
ACCESSION   DAELTA010000093 DAELTA010000000
VERSION     DAELTA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.093"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            199..1545
                     /locus_tag="KPG13_002409"
     CDS             199..1545
                     /locus_tag="KPG13_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0986119.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            1572..2972
                     /locus_tag="KPG13_002410"
     CDS             1572..2972
                     /locus_tag="KPG13_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0986120.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            3182..4618
                     /locus_tag="KPG13_002411"
     CDS             3182..4618
                     /locus_tag="KPG13_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG0986121.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            4962..5906
                     /locus_tag="KPG13_002412"
     CDS             4962..5906
                     /locus_tag="KPG13_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG0986122.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            6075..7007
                     /locus_tag="KPG13_002413"
     CDS             6075..7007
                     /locus_tag="KPG13_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG0986123.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            7112..7579
                     /locus_tag="KPG13_002414"
     CDS             7112..7579
                     /locus_tag="KPG13_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG0986124.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(7823..8677)
                     /locus_tag="KPG13_002415"
     CDS             complement(7823..8677)
                     /locus_tag="KPG13_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBG0986125.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            8843..10807
                     /locus_tag="KPG13_002416"
     CDS             8843..10807
                     /locus_tag="KPG13_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0986126.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            10807..11268
                     /locus_tag="KPG13_002417"
     CDS             10807..11268
                     /locus_tag="KPG13_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0986127.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            11271..11591
                     /locus_tag="KPG13_002418"
     CDS             11271..11591
                     /locus_tag="KPG13_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG0986128.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            11607..12707
                     /locus_tag="KPG13_002419"
     CDS             11607..12707
                     /locus_tag="KPG13_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG0986129.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            12827..15427
                     /locus_tag="KPG13_002420"
     CDS             12827..15427
                     /locus_tag="KPG13_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG0986130.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6223 a         1616 c         2719 g         5109 t
ORIGIN      
        1 ttgtattaaa aaattaagaa taacttggta atagtgaaaa tataatgaga ttataattga
       61 tttataatat aactataatg atgttgtaaa atgaaaattg tcatttcaaa attcccaaaa
      121 agagtaaaag tcacttcggt tatagtcaaa aaattttttc agtgaaaaga atttagattt
      181 aaaagggagg aattaatcat ggaaattgaa actaaaactc cggattggat taatgtacta
      241 gaattaaaca gtaaaataga tattactggg aaatatgatg taagtaatat tatacaagat
      301 attgtctctg ataagtcact agaaggttcc attatatatt ttccaaaagg taattattta
      361 tttgagaaag gtattaaaat tagtcaacaa ataacacttc aaggagattc ttattatgga
      421 ggagataatc aagtatcaaa tttagataaa aaacaatcaa tcataggaac aaccaatttt
      481 ataactcgag gagtttctaa tatgagtatt attgcattga caggaacaag tcaatgtatc
      541 aaaaatatta atttttatta tgatagtcat gatatagaga aaataccgcc taaaaatgta
      601 tctgctatta cagaatatgg agaaacacaa ggactatctc attttgaaca cctgtttatt
      661 tctggatttt cagggatagg gattgaaatt ccatactatt caactggcaa tgatataatt
      721 gttagttcat gtggtcttgg tatgaggctt ggagagaagt caatgctttc tagtagcaag
      781 atttacgaat gtaaaaatgg aatggagatt actactggtg ttagcttaaa taatgtgaga
      841 atagaagaaa taaaagaaat aggtattaat aataaaggat ttggttttaa cctgattatg
      901 aatcttatgg tcgaccaatg tggatattgt ggatttatgt ttgaaaaaat gtgctatagc
      961 cagataactg ccagaattac tagatgcggt cagtattata aaagtgttga ttataatgct
     1021 tatgagaata tggaagatag aaaagaaaaa gcttattcca ttttttatgg agatatttta
     1081 gaaagttgca atattgtatt gatgaataga aatattgata ccttagaaag tgattcactt
     1141 aatgaacata agatatatgt aatcaaggca aatgaaacaa acaatgtaat gctaacatgt
     1201 tatgctgaag cagataactt tatacaatgt gcaaagggca acttgctgtt agaaaatggt
     1261 gaaaatactt ataaatttta taatggtgag atttgttctg taggaggtgt tcaaatttct
     1321 gatatagatg aaaaagattt aatcaaaata atagagaaca ctttgtatat agatgataaa
     1381 ggtaagtatt taatggttcc tagattaaaa aagaatactg taatatattc agtactagat
     1441 tctacagaag aaataaataa attacatggt ggaatatggg aacagattga tgtaaaagta
     1501 ttagctgggg aatccatgta ctattataaa aagataaaag attaaattat caaactagga
     1561 ggtagtgatt aatggaagag tctataataa ttaaagatat aagtggctgg attaatgtac
     1621 ttgattatgg aatttgtaat gatgaaacaa aagatgtaag ttttaagttg caagagatta
     1681 tggatgctgt accagaagat tctattatat attttccaaa aggaaaatac ttatttgaaa
     1741 gtggtgttaa gattaagaaa cgattaacct tatgtggaga ttcttatatt ttagcgaatg
     1801 ttcctaaaaa tactgctgga gtaacacagt ttacttttaa aggaaagaaa aatcctaaaa
     1861 gtgaaaatag agcaatcata acagcaaaaa gtgtaagaca ctgtattaag aacatttgct
     1921 tttattcaga tagttgtgat agagatatga catctaatca tgaacctcca acagatggaa
     1981 aacctagatt tcatcataat gtaattataa attatagaaa tatatctgct gttatatgtg
     2041 aagataaaca gggagaacca ggtcattatg aaaatctatc ttttagtggt ttttctggaa
     2101 ttgcacttga tatgcctaat aattctacag caaatgatat tacaattttt acatccggac
     2161 ttggaattag tacaggggaa aattcaacaa ttgctaacag taaggtttgg ggctgtgttg
     2221 atggaatgat aatttctact ggaactttta taaatggtat gagggtagaa gaaataaaaa
     2281 aagttggaat taaaaatatt ggtagaggtt taaattttat tacaaatatc acaattgacc
     2341 aatgtggata ttgtggagtt tggtttgatt ctatatccaa tgttcagata tcaggaaata
     2401 tcacaaggtg tgggcagtac tattatgatg taagctatga taattattta atgttggata
     2461 atagagttaa agaagcatat tctttattct atggtaattt attggagtca agtaatatag
     2521 ttttatcaaa taccaatgaa gataattggg aagatttcaa agaagataag cataaggtgt
     2581 atcttataga agcacttaaa accaagaatg tactattaac atgcaatata gatgctactg
     2641 actggattgt taagtcagaa aaaggaaatt taacgtacaa taatatgcga caaactgtta
     2701 tattttacga tggaaaacta agtagtatca atggaattgg tatatctgat gaagattatg
     2761 gggataaagt caagataaaa aaaggaaata tatatataaa tagtaatgat aaggatatta
     2821 agatgcataa accagaggta ggtgacatta tgtcaacaat tattaaagat aaaagtattc
     2881 tttctgctca ttatggggga acatgggaga aaattggtga aaaaatagag tttggaataa
     2941 ctgtacacta ttataaattg atagagaaat aagtaaatac gtagtatttg aatagatgta
     3001 ttttattgga gagttaggac tatagtttta atataaatgg tttggtaata gcatggattg
     3061 aataatataa aaataatata aaaaattaat ttgattatgt aaatttctat gaagtagtaa
     3121 aactgtgact ttagtcatag gaggttcaaa ctaagtaaaa gggaaaggaa tatataatat
     3181 tatggaagga agaactggga ttaaagatgg aagatggatt aatgtagtag aaagaggtat
     3241 taaaaatgat ggttcgtaca acataggtcc tgctttgcag gatattattg atgaaataga
     3301 aacagatttt tgtgtcttat attttccaaa aggtaaatac ctatttacaa gtggtgttaa
     3361 aattaataaa gaaattgtat tgcaaggtga ttctaattca tctacaggta ctacacaatt
     3421 tataactcgt ggagtagaaa atatgagtat tattacattg acagggaaga aacaatgtat
     3481 taaaaatata aatttctatt ctgatagttg tgagatacag gtaaataaag aaccaccgac
     3541 aaaaggtaat cctaaatatc attatgagat gattattaat aagaatgaaa agaatgttga
     3601 gatgaataat gtatcagcaa tcctgtatga taacagtaag aatatgatta aaggtctagg
     3661 tcattatgaa aatatttata taagtggttt ttcgggtaca ggaattagga taccatatta
     3721 ctcaattgta aatgatatta cagtttcatc ttgtgggctg ggtattgata caggaataga
     3781 cacaattatt tctaatagta gaatatctaa gtgtcaaaat ggaatgagga ttagaactgg
     3841 aactagtata aataatgtaa ggattgaaaa aatacagaaa gttggtctta taagtagtca
     3901 agttaagggg tatgaggggt atggaagtta taaaattaat aatattacga ttgaccaatg
     3961 tgggtattgt ggcttttcat ttgattcaat gaaagatagt tatttttctg gtattataag
     4021 aaggtgtggt cagtattatt ataatacaga ttatgacaca tatttaagta ttaaagatag
     4081 agtagaagaa gcatattctt tattttatgg aaattatttt acagaatgta atattgattt
     4141 agttagtgat aataaggata actgggatga tggcttagaa tataaacata aagtttatgt
     4201 atttaaaacc cttcaaatac caaaattaac attgagatgc aatatagatg cctatgatta
     4261 tatcgttaaa tcggtacatg gaggaggtaa tttaatgtta aaaaataact ttaacaatta
     4321 tatatttatt gattcatcta atccagaagt tattaatgga attaatttta attatcaaaa
     4381 gaatggaaac ttgttaaggt taaataatgg taaattaagt tataaaaatg gaaaggaaga
     4441 aacagctata ttcagaccta attatggtct tattgtagca tcaacagatt cagaatgtga
     4501 acaaatagag gctacctatg gagaaaaatg ggagaagctt ggttctgaag taattggaga
     4561 agaaacggta tattattata aacatcatga accagataaa acaaaccaaa tacaataaaa
     4621 tttgtattat ataatgatat aaagttttaa tatatagtgt ttacctttaa tgtaatattg
     4681 gtatatttgt atagtattaa atttgttaaa aagtaaaatt gtagggtgct tgaaaatagc
     4741 acctttttta gtttcattat aaaattagtt ttgaaagttt aggaataatg tatatttatt
     4801 tttaaagaat tataacctta ttaaggatat tttaaatgct agaaatgaat cagtaaatca
     4861 tactggaatt atcttaaatg ttttgattaa attaagtatt tagagctata attatattaa
     4921 aaatatatta gaacataatt atattactat ggaggttaaa tatgatagta agtgtgagta
     4981 gaagaactga tatacctgct ttctatagta agtggttttt taacagactt gaagaaggtt
     5041 ttgtttatgt aattaatcca atgaatccaa aacaggttag taagatagag ttaaatccac
     5101 atactgttga ttgttttgta ttttggacaa aggatgtaac tccaatgtta tatgatttaa
     5161 ataaattaag agactataaa tattattttc actatactat aacatcatat ggaaaagaag
     5221 tagagacatg tattttagat aaaagaaagg taatagattc atttaaggaa ttgtctagaa
     5281 aaataggcaa agaaagagta attttaagat acgacccgat tttcttaagt gaaaagtaca
     5341 cagtagatta tcatatcaaa gcatttgcga gtttatgtaa tcagcttgat ggatttacag
     5401 aaaaatgtat aataagtttt atcgatttgt ataagaaaac aaaatttaat actaaagcat
     5461 tacatataaa gccaatagaa attacagaaa tagaaacatt atcaaaagag atttctaaga
     5521 ttgctcataa gcatgggata atacttgaaa tgtgttctga agaatataat ttaagtaaat
     5581 ttgggattgg aaaaagtaag tgtattgatg atagattaat atcaaaaatt ataggatgtg
     5641 aagtagaggt aaaaaaagat agtacacaaa gagatatttg tggatgtgtt aaaagtgtcg
     5701 atataggaca atacaatact tgcagacatt attgtctgta ttgttatgct aactttaatt
     5761 atgcaaaagt agaagaaaac tgtaggcttt ataaggaaga taataaatta ctcatagata
     5821 ctttaagaga tgatgcaaaa ataagcatta gagatatgaa gactattaaa gtagataaga
     5881 ataaacaaat tagtatattt gattaaaata aatatactaa ttaccttaat atattaagaa
     5941 tatagatact tttgataaca aaattaaact taatagatat tttctatata agaaagatgg
     6001 tcataatatg tcaatttgac catctttttg atataattaa aacacaaata atttagtaac
     6061 aaaggaggca gaacttgaat ttattaaaaa aatatcctat acctattgca agtttgattt
     6121 tatcaatttt tactttaggt aacttattac aatcttatag tgaatcatta cgtaatgtaa
     6181 ttggatttat aggatttatt ttgtatgcaa tttatatagt caaaataata ttacttagaa
     6241 agggtgttaa agagcaatta gagaaccctt taatatcaag tacatttcca actataacaa
     6301 tggcaacaat gatatttgct acatatataa aaccattatc tttagaatta agtattatga
     6361 tttggagcat aggaattgtg ggtcatcttg tgttaatagt cttatttagt aagaagttct
     6421 tagttaaatt ctcaataaaa acggtatttc catcttggta tatagtatat gttgggatag
     6481 ttgcagctag tgttacggca ccagcattta atcaactttt gattggcaaa attgcctttt
     6541 ggataggatt tgtatcttat atagttctta taccagtaat tttaaagaga gtatggataa
     6601 tcaaggaaat gcctgaacca tccttaccaa gtatagctat attagctgca ccagggtcac
     6661 tattattgtc tggatatatt aattcttttt cagataaaaa taatgtagta ttatacttgt
     6721 tattcatatt atcaattgta ttttatatta tagttttggg atatttaccg aaattattaa
     6781 ggttgccttt ttatccgagt tttgctgcat ttacattccc tatggcaata agtgcaatgg
     6841 gtatgaaact aatgaatggt tatttaacta aatcaggtca ttttataagt tggatttcat
     6901 atgcttctaa gttagaagag ataattgcta cagtgattat tttatatgtt ttagttttgt
     6961 ttattatata tcttagtaaa tctgaaacta catctaaagc taaataagaa ttattaaata
     7021 tgtgtattta ataggaatat tgatataaat atatgaagat aagataaacc atagaaactt
     7081 aatttagctt aattttattg gaggaaaaca aatgaaaaca aaagaagaat atttaaaaga
     7141 agttagaaac atatcagaag aatactttag aaaaggagag tttttttgct ctgaagcagt
     7201 tctccagaca ataaatgatg ctttaggcca acctttaagt ccagagataa ctaagttagc
     7261 atcaggattt ccgataggac ttggaaaggc acaatgtctt tgtggagcaa tatctggggg
     7321 agagatggct ttaggtatag tatatggaag agttcatggt gaagctatga atccaaagat
     7381 gtttgagcat gctaaaggtt tgcatgatta tattaaaaaa gagtatggtg caacatgttg
     7441 tagagctatt acaaagaaat gggatggtga taactttatg tctcccgaaa gaaaagagca
     7501 ctgtattaaa ataacaggtc aagttacaga gtgggttgca gataagctta tagaagatga
     7561 aaaacttaat atcaaatagt gtaatattag ttggtgaatt tataaagtta aatttactta
     7621 tagtataaga tacttgatat tctcgtattg atagaaaaat ttatagcata tgtatttaat
     7681 gaaatatact tatatttaga gtgtggttta atttcataaa tacatatgct attattttaa
     7741 gtatataagc aaatttattt aaataaatag aaacagttaa tttatatgta caaaactata
     7801 tatgattagt gtaaataaat acctatttta taatactcaa cataaagaca ccagacatga
     7861 ttagtattat accaataaaa atagcaagcc attctttttt gttcttcttt tctttaaaaa
     7921 atataatccc tcctaaagtg gctactatca cacaactctg agagatagaa aaacttattg
     7981 taactcccaa tgtattagtc gttatgaaca tacctaaatt tgctattgac cacaatatac
     8041 cagtaattaa attgaataaa acattttccc tatatacaat ctctttagaa aaatacttga
     8101 caataataga tgaagttatc aacataccaa cagattgagg taatataatt ctatatcctt
     8161 caattaaaaa tatttgatta gttacaacat ataatgataa aaatagagat gataataata
     8221 caactccaac acattttaac atatttttat ctgtagattt tttagtttga taatttgtga
     8281 gaagtatacc aagtattata cataatattg ataaagaacc aatcagtaac attttagaac
     8341 tggtccattc attaaacaag attacagcta ataatgtcgt aaaagttaat tgtgaaccat
     8401 ttgaaattgg cattacttta gatacactag caatttgaat tgctttaaac tgcattaatt
     8461 gtcccaaact ccagaatatg ccagaaatca tacttataca aaatgaaaaa aatgtaattt
     8521 caggatgtat aaccagagtt attatagtgg aaaatataaa cgcacttatg gatgtaccta
     8581 aaagctgttc atgaggagaa cttttcttta gatttgcaac tatgggcatc aatccccatc
     8641 ccattacagg aagtaaagca ataattattg tcagcattaa actatttcct ttcatattaa
     8701 atttagacag cttttaattt atcatctaaa attaaagtgt tcaatacata aaaattaccg
     8761 tatttaatca ggaaatatta gtagtttttt agaaaaacat tttccattaa aataaagaca
     8821 aagggaagga gtaaaattga aaatgttaac tgatatgatt caaaatcgtg aaattaaaat
     8881 aataaagttt ttattaaaag aaggtcatac aactgtaaaa gcaatagcag atgatttaga
     8941 tgtttcagaa aaaacagttt caaaatctct taaagaagta gaagcagtgc ttaaaaagat
     9001 aggtctttct ttaataagaa aacctaagat aggtgtttat attgatggag atttaaaaaa
     9061 aatacttcct tacctagata ataaaggaag tcaaattcct acaaccagag aggagagagt
     9121 tatctatata tattcagtac ttttaaagag taatgattat attactatac aacaactttc
     9181 tgatgaacta tatataagtc gtggaactat tgaaagggat ttagcagaag ttgaaaaact
     9241 gctaaaaaac gaagggattt ccatatataa gaaacctagt aagggaataa agttaaatat
     9301 aagtgaaaga gataaaagac aacttactgc taagtttata cataaatttt ggagtagaaa
     9361 ctggtatatt aagcaagagc aagaaagctt ttatcaagca tttgaaaaga tacaagcaga
     9421 tgtaaatggt attttttcgg aagatgattt atcaataatt attgatattc ttaggaattt
     9481 cagcagagaa agaaaatttg agttctctga ttatgaattt cagtcattag ttattcattt
     9541 ggcaatagct gtagagagaa ttagaaaagg cgaatatata gaagaaagtt tgtatactgg
     9601 taatatagaa aataatcaaa tgtcaaatac caattattta gcaaaacaat tagaagaaaa
     9661 attttcaata ttattaccaa aatctgaaat tggatatata aatttacatt tagtagcgtc
     9721 tgaccaacat agagatacta atgaactaaa aaatacttca actaatcctt gtgagacaag
     9781 tacaaataat atatcaaatc ttcgggaaat tataaaaaat acattgtatg agacagctta
     9841 tgacaaggat ttaattgatg gattaacttc acatgtacat tcatcaataa atagactgaa
     9901 atatggactt agtattaaaa atccatatgt aaatacaatc aaacaaaact tttctttatc
     9961 atttgagcaa gctttagatt taaagaaagt aattgaattt atatatgata ttaggataaa
    10021 tgatgatgaa tgtgcttata tagcattaca ttttgaagct tttagagaaa gaatgaaaga
    10081 acttccacaa aatattagag taattctagt atgcagtaca ggattaggtt catctaggtt
    10141 attgtctgca agaataaaaa aatactttcc aatgataaag attgaaaaag tactttccgt
    10201 acaagaatta gtatccagta aaattgatgc agatttagta ataagtacta tttatttaga
    10261 gttagaaaca attccaacca tagtagttag tcctatttta aatgaaagtg atataagaac
    10321 cttggaaaga aatatacaaa atgtttcaag ttatacttct aagaagtata aatctttcag
    10381 aaacttaatc tttgaagaaa atatattgtt gaatattgaa gcttcaaact atgaagatgc
    10441 aattaagtat ataacagata acctaataaa aaaaggtttt gcaaaagaag gtatagctaa
    10501 aagtgcaatt gagagagaaa atttatctta cacttctttc caatcaatag ctacacctca
    10561 tgctaatcct agttatatta aaaaatctaa tatatctgtt gcaacgttaa aaaatcctat
    10621 tacatgggga aatgaaaagg tggaagttat tttctttatt tcattagaaa atgacaaaaa
    10681 cctagagctt gatgaaatat atgaagtttt ttatgacttc attgacaata aaaaaaatat
    10741 aaatgacata ttaagtgcaa cttccacaaa ggaagtgtac gatttgctag tggaggagag
    10801 tatatagtga aaatatcaga tgtattgaaa aaagagcaaa ttgtttttaa tcttgataca
    10861 aatacaaagg aagaaacaat aaaaaaatta agtagtatat ttgttgaaaa tgggattgtg
    10921 gacaatgaag aagaatatgt taagtctgtt ttggaaagag aagaacattc aactacaggt
    10981 attggtaatg gtattgctat tcctcatggg aaaagtaatg ctgtcaaaaa atcttctata
    11041 atatttgcaa agactaagca taagattgaa tgggatgctt tagatgaaaa accagtagat
    11101 agtattttct tattggctat aagcaatatt gataaggata gcaatcatct tgtactatta
    11161 tctaaattag caactaaact tatggatgat gacaatgtag atgcactaaa attagccaat
    11221 tcagaacaag aaataatcaa tatatttagt gaagaaggag agatgtagaa atgaaaagaa
    11281 aaattatagc agttacagca tgtgcaacag gagttgcaca tacatatatg gcagctcaag
    11341 cgttaaagaa agcttcaaaa aagatgggtc accttataaa agttgaaaca caaggagcta
    11401 caggaatcga taatgagtta actacaaagg atgcacaaat tgcagaagta gtaatatttg
    11461 cagtagatac taaagtcaga aatgctgaac gttttgaggg aaaagaaata ctagaagtac
    11521 cagttaatgc acctataaag gatgcagaga aagtaataaa tgatgcacta aagttaattg
    11581 acactaaata aatggaggga aatgtaatgt tgaaaaagat agtctctgag ttaaaaaagc
    11641 atgtcttaac tggaatctca tatatgattc cattagtaat tgcaggtgct atgataatgg
    11701 cgatatctcg tgtaggtggt tctttctata atataccaga tatatgggat gctaaatatg
    11761 cagaaagtgc tagtagtatt gttagattat tacatgattt agatgggttt ggaggtactg
    11821 cattaggatt aatgtttcct gtaattgctg catttatagg attttctata gctgataaat
    11881 tagctattgt accaggtctt gttggaggta tgattgctaa agatataggt gcaggatttt
    11941 taggagcttt ggcagttggt cttatagcag gatatacatg tttgtttatt aaaaatcatg
    12001 ttaaattacc aaaatcagca gcttcaatag taccaatatt tatagtacca gtatttggga
    12061 cattaatcac tgttgtatta atcaattatg tgataggtat accttttgcc acattaaata
    12121 ctggattaga aaattggtta aatggattat ctggtacaaa tcagatacta atggcagcta
    12181 ttataggggc tatgatggca gttgacttag gtggaccagt taataaagca gctttaacaa
    12241 catcacttgc attattaaca agtggaattt atgcgcctaa cactgctgca atggtaggaa
    12301 tagttatacc accactagga ttgggacttg ctactatact agcaaaacaa aaatataata
    12361 aacagttacg tgaagctgga aaatcatctc ttataatggg tttaattgga gtaagtgaag
    12421 gagcaattcc ttttgcggta gaatctccat taaaagtaat accttcatgt gtaattggaa
    12481 cagcaattgc ttcagctatg gcagttggtt taggttcagt aaatgcaact ccaattagtg
    12541 gattttatgg atggtttaca gtagagaatt ggccaatgta tgtgttatca atagcagttg
    12601 gaacagtaat tgtagctgga ttagttatag tattgcgtgg aaatccagtg attgaggatg
    12661 atgaatttga agatgatgat tttgatgaag cagagtggga atcttaaaat ttaataacta
    12721 tttaagacgt gttataagct atattcaata tgaatcttat actaaataaa gtgttaaaca
    12781 ttcagtttag acttaaatat aaaagataaa gaacagagga gaataaatga taaaagcaca
    12841 tattgttaat catacacact gggatagaga atggtatttt acttctggag atgcattagt
    12901 acttagtgaa caactattta cagatgtaat tgatgaatta gaacgtaatc cagatgtaag
    12961 ttttgtactt gatggacaac tttctatttt ggatgattat gtacaattac acaaagaaaa
    13021 aatagaaatc attaaaaagt taataaaaga agatagacta catattggac catggtttac
    13081 tcaaactgat gcattctttg ctagaggaga gtctatttta agaaacttaa tgattggaat
    13141 ttttgaaagt aaaaagtatg gtaagtatat gaaaattggg tatttgccag atacatttgg
    13201 atttaatgca cagatgccta tactattaaa gcatgtaggt cttgataata tattcttttg
    13261 gagaggtata catcttggta aacaggttca atcaccatat tttaagtgga aaagcttaaa
    13321 tggagaaagt tatatatatg cagtaaatat gccacatgga tatggaactg gtatgctttt
    13381 agagccttca ttaaagtatg ttaatggacg cttagaccca gctattgatt ttattaaatc
    13441 atatactgat gtagatgagg tgttaatacc ttctgggaat gaccagttaa atataatagg
    13501 tgatttcaaa aataaaatta aagcgattaa tgatatcgga aagtattcat atataacgag
    13561 ttcatatcaa gagtttttaa aatatataaa gtccatagaa tcactagaaa gctatagagg
    13621 agaatttaga gaacctgtat tagcaagaat acataaatca attggttcta gtagaatgga
    13681 tataaaatta gcatgtgata aactagaaaa caagctgatt aaaagaatag agccattatt
    13741 agtaattgct aaaaagagta aaattgaaat aagcaatcag ttacttataa atacatggaa
    13801 aaaacttttg gaaggacaag cacacgatag tttagctgga tgtgtaactg atactgtaac
    13861 agatgatatt cttcaccgta taagagaagc aaatgaaata tgtgacagta ttgaaaatac
    13921 tattgtaaaa aagatttcag aagggcttaa attatctaaa aatgatattt tagtatttaa
    13981 tacagaagct aagcgtttta atggatataa agagatacaa gttgtaagtg atagtaaaaa
    14041 tatttatttt aaagataatt tagatgctac tataattgaa gaaacttatg taaaaccgag
    14101 agaaaatgta ttagaagaaa cacctgctgg taatatattt attgaagaac caggatatta
    14161 tatattaaaa gtaagaataa atgtacagct tcctgcactt ggttataaag ttgttagttt
    14221 tgaatcaagt gataaagaaa tggtgtcact taataaatct aatgatgctt ttatatctaa
    14281 tgataattat aagataatat atgaagatgg aagcttaaat ttacaactta aagatggaac
    14341 ttacatatca aactttttaa cattaaaaga tagtggtaat gcaggtgata cctatgactt
    14401 ttctccacta aaaaatgatg aggatataaa actttcgttt gataaagtgt atacagaaaa
    14461 agctttagga tatgaaaaaa tggtggttaa aggtactact ttactaccac ttacgttaga
    14521 agatagaaaa aacaaatatc ttaatgggaa attaaatgta aaagtaagta ttgtcttatc
    14581 caaagaaaat ccattgatgg atgtaaaact ttgtgttgat aatacgatat ataatcatag
    14641 actacgtgtt catataaaaa ctgatataaa agataataaa aacatagcat cattgccatt
    14701 tggatatata acaagagaaa atggagtttt agacaactgg gaagatattt acagtgaaat
    14761 gcctatagat ttagaacctt tggaaagtaa tattactcta acaaaccaaa atagaagctg
    14821 tactatattt actaaaggaa taaaagagta tcaacatatt gaagatgaaa ttgcattgac
    14881 cttacttgct actacagatg aactaggaaa gcctgattta ttatatagac caggacgtgc
    14941 ttctggtgat acaactaaaa aaggtcacat aagaattaaa acagataaag ctcaaatgct
    15001 taagaaatta gaattttcat ttgcaatata tatggattct aaatcatttg atgaattgga
    15061 aattgcgaat ttaacccaca attatctaag ggaatgtgta aattatcaat tacaggatta
    15121 caatttcttt ttatatcgta tagataataa gattcaaaaa tcaattgtca ataaaactgt
    15181 aagtagagaa ttagaaataa tatctttacc agaaaactat cttatatcag catgttatcc
    15241 ttcttattac agtaaagata aatttataat aagagtggaa aatccaagta gtaataaaat
    15301 gatattggat gagcaaatat ttaaaaatag aaatgggaaa attgtaaatg caatagaaga
    15361 tgtagaagag gaacaggtat atgaaatagc accttttgac gttatttcaa tattattgga
    15421 tttataagtg taaaagtaag tttattgttt aaagtctgac agatattaat ctgccagact
    15481 ttttaatttg tataaatttg ggtaaatatt aatgaatggt tttagtattt aagtatgaat
    15541 ttagtattta atatagaatt tattgaagta gaataattag tttaacagga taagcagttt
    15601 aaataaagaa ttattatatt agagtgattt tatatataat ttaagataat aaaattttaa
    15661 taaaatg
//
LOCUS       DAELTA010000094        15665 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.094, whole genome shotgun sequence.
ACCESSION   DAELTA010000094 DAELTA010000000
VERSION     DAELTA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.094"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            81..719
                     /locus_tag="KPG13_002421"
     CDS             81..719
                     /locus_tag="KPG13_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986131.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1192..2550)
                     /gene="rlmD"
                     /locus_tag="KPG13_002422"
     CDS             complement(1192..2550)
                     /gene="rlmD"
                     /locus_tag="KPG13_002422"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG0986132.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2725..4485)
                     /gene="pyk"
                     /locus_tag="KPG13_002423"
     CDS             complement(2725..4485)
                     /gene="pyk"
                     /locus_tag="KPG13_002423"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG0986133.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4537..5496)
                     /gene="pfkA"
                     /locus_tag="KPG13_002424"
     CDS             complement(4537..5496)
                     /gene="pfkA"
                     /locus_tag="KPG13_002424"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0986134.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5705..9274)
                     /locus_tag="KPG13_002425"
     CDS             complement(5705..9274)
                     /locus_tag="KPG13_002425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG0986135.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9431..10378)
                     /gene="whiA"
                     /locus_tag="KPG13_002426"
     CDS             complement(9431..10378)
                     /gene="whiA"
                     /locus_tag="KPG13_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG0986136.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(10395..10820)
                     /locus_tag="KPG13_002427"
     CDS             complement(10395..10820)
                     /locus_tag="KPG13_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0986137.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10889..11995)
                     /locus_tag="KPG13_002428"
     CDS             complement(10889..11995)
                     /locus_tag="KPG13_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG0986138.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(12007..12864)
                     /gene="rapZ"
                     /locus_tag="KPG13_002429"
     CDS             complement(12007..12864)
                     /gene="rapZ"
                     /locus_tag="KPG13_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG0986139.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12895..13608)
                     /locus_tag="KPG13_002430"
     CDS             complement(12895..13608)
                     /locus_tag="KPG13_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG0986140.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(13612..14526)
                     /gene="murB"
                     /locus_tag="KPG13_002431"
     CDS             complement(13612..14526)
                     /gene="murB"
                     /locus_tag="KPG13_002431"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG0986141.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14799..15569)
                     /locus_tag="KPG13_002432"
     CDS             complement(14799..15569)
                     /locus_tag="KPG13_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG0986142.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4815 a         2990 c         1710 g         6150 t
ORIGIN      
        1 taattttaaa agaggagaat gtatataaga gtattcaatg tactagaaaa aatcaaaaga
       61 aatattagaa gaaatggata ttgagtaatc aaagaaaaga ttttgatatt acaaatgata
      121 tgtttcaaga aagcattcct atcacaacaa aaattattct tgaagacatg ccttcaaatg
      181 atgaattaaa tcatgttttt tcaaaaggtt gtgaacgaaa aatgaaaaag agaaaaattg
      241 tattaatcac actactatta ataggtgttt tgttattagg aagtatccta tataatcttt
      301 ttttaggaaa aacagctaat atttcaatgc taaaggaatc ttggaatttt gatataccaa
      361 tacctaacaa agaaattgaa gtatttgata ctcaagatag tattaatgga gatggacaaa
      421 gttattttat tcaaggattt tctgaaaaaa atttcaaaaa agtttttaat ttaaaaggtg
      481 gtatagttgt aagcaaagat aatattaatg aaatagaaaa atatattgat aaatttaaaa
      541 gagattctgt taacattaat aaaagtaata aaaataaaat tgaagaagat tttaaaaaat
      601 ataagttaga agttaaaaaa gatgataagt atatatataa gagaaattat gagaactacg
      661 tagttttaat tataaaaaaa gatgaacaaa aattatacag tttaatatgg aatcagtagg
      721 tacatatgta ttattaagaa ttataaataa taaaaataaa ttttagtcaa tatacaaaaa
      781 ccttttaaat tgtctggaaa tcacctcttc atttgttata aaaacataca acaaatgaag
      841 aggtgatttt atggttttaa aaaaaataat tataagtgga tatgtgcttt cacatgttta
      901 tcaacatttg taatgcctat atttgctaat gaattataaa aatattaaat aatattcaac
      961 tatagattat gtaaattact ctatgaaaaa aatgagttgt ataaaatctg gctactgtag
     1021 attgactatt tcttttaaaa ttacaattat aataagtttt atagaattaa aaataaaact
     1081 ttagagattt ttgataacat tgtagactaa atttgccacc gaaatcgctt tacagtggca
     1141 aatcggtggc aaatacaata tattattagt atataatcta tataaacatt actattgttt
     1201 gattatctta gctacagttt ccacatgtgt agaatgaggg aacatatcta caggttgtgc
     1261 ctcaacacat ttaaatccgt gaacatttag ataatctaaa tcccttgcta aagtagaagg
     1321 attacaagaa acatagacaa ttctttccgg attcattgaa acaatagtct ctaatacctt
     1381 ttcgtcacat ccctttctag gtgggtctac aacgactaca ttggcagtct taccctctct
     1441 atacatctta ggtacaattt cctctgcctt tccaacataa aattcagcat tatgtatatt
     1501 gtttaattta gcatttatct tagcatcttt tatggcatca tctataattt ctattccata
     1561 aaccttttta gccttttgtg ccaaaaataa agatattgta cctatcccac agtaaatatc
     1621 aaaaacagta tcagtatcat ttaaatttgc atattcaaga gccttatcat acaatacttt
     1681 agtttggaca gggtttactt ggaaaaatga taatggtgaa atctcaaaca ctaagtctcc
     1741 tatataatcg tttatttttc catctccata tataactata ttttctcttc ctagaatagc
     1801 attcgtatct ttttgattta catttacaac caaagtctta aatccaggta tattttcctt
     1861 aagcactgaa gctaattctt taagatatgg aagtttgttt ccatttgcta ctaatacaac
     1921 cattacttct ttagttgtaa aacctatttt tgttactaaa tgcctaatca accccgtgtg
     1981 agtcttttca tcatatatgc ttaccttata ggcccttata tatgttttta ttattttaac
     2041 aattttatca tttatctcat gttgtattac gcatttttct gtcgatatta catcatgagt
     2101 cttcttttta tagaagccaa taattggaat accattattc ttttgtattg gaaattgtgc
     2161 tttatttcta tatctatatg gattatccat tcctatagtg tcatgaactt ctacatcctc
     2221 taacttacct attctcgaaa gtacttgttt aacttcattt gttttcattt caagttgttt
     2281 attataatct aagtcttgta tctgacaccc tccacaatcc ctaagattgt cactacacac
     2341 tcttttcacc ctgaatggag acttttctat tatctcaaca atatctccca cagcataatt
     2401 tttctttact ttattaatcc taactttaac tttatcatgt attaatccgc cctctacaaa
     2461 aactgtaata ccattgtact ttcctactcc tactccgcct tgaccaatgt ctactatatc
     2521 tacaatatac atctcgtttt tcgaaagcat aaatcaccct ccaatcaatc tattatattt
     2581 aaatctttac aaactttttt ctttaaattt atattaaaat attgctatta attaaactat
     2641 attttttact aagctacaat attttatttt accaaaaaaa aaggagctta taaagcccct
     2701 tagtaaataa atttattatt tttcttataa aactctagtt gaacctctat ataccacgcc
     2761 tcttgatgca tccacagtta caacttctcc atcttttact agatttgtta tatcagctgc
     2821 agacacaata actggtatat ctaaatttat acctacaata gctgtatgtg atgtcattcc
     2881 accattctca gttactacag ctcctgcttt ttctatataa ttgttcattt cagcatcagt
     2941 agcagtagaa acaagtatgt cgccttcatt aaagtctaca caagcatttc cattctttat
     3001 tatacgaact ttaccttcta ctgtttgagt acctacacct ataccttgca cgatttcttc
     3061 gcttattaca tgaaccttta ttaaattagt agttccactt actcctactg gtactccagc
     3121 agttataact actaattccc cattatctat ataatcagct tgtctagctg cctcaattgc
     3181 cttttctata acttcatctg tatttcctgc aacagaagac ttaactggat atactcccca
     3241 tgtaagagct aatttattca tagtcttttc attatttgta gtagctatta ttggagattt
     3301 tggtctgaat ttagatacca ttctagcagt atatcctgaa gaagtagatg ttattatagc
     3361 tgatgcattt aaatctactg ctgttgtaca agttgcatgg cttattgcat ctgttacagt
     3421 aacattgtta gtaccatttt cctttagtaa tctatcataa tctaaagtct cttctgttct
     3481 ttttgctatt gtagccatca tttttactgc ctctacagga tattttccag cagcagtttc
     3541 tccagataac attatcgcat ctgttccatc atatatagca ttggcaacgt ctgtaacttc
     3601 tgctcttgtt ggtcttggat ttcttatcat agaatcaagc atttgagtgg cagtaacaac
     3661 tggctttgct aattcattac atttttttat catcattttt tgaactattg gcatctcttc
     3721 agttggtatc tcaacaccta agtcacctct agccaccatt ataccatcag aaacttttaa
     3781 tatttcatct aggttctcta caccttcttg gttttctatt ttagatatta tttgtatatc
     3841 agttgcatta ttgttctcta atacttctct tattgctagt acatcagatg cctttcttac
     3901 aaaagaagca gctatataat ctattccttg acttattcca aactctatat cacttatatc
     3961 tttaggagtt attgctggta aatttatctt aacaccaggt acatttactc ctttatgatt
     4021 tttaactatt cctgaatttt caacaacaca aactatatct tctccattta tttcttttac
     4081 tcttaagcca actaaaccat catctattag tatagaatct cctgatttaa cgtcttcagc
     4141 aagaccttta tagcttactg tacacatttc tttagtcccc ataatatctt tcatagttat
     4201 agtgaacttt tgcccttctt ctaaaaatac ctctgggtct tcaaagttac ctgttctaat
     4261 ttctggacct ttagtatcta ataatatagc aactggctta tttaattttt ctctaacttt
     4321 ctttgccata tcaattcttt ctttatgttc ttcatgcgaa ccgtgagaaa agttaaatct
     4381 acacacattt agaccatttt gcataagttg agttaaaact tcctcacttt gtgatgctgg
     4441 acctagtgta caaacaattt ttgtcttctt tacattattt aacatatttg ctcctcctga
     4501 gatattattc cacagattta tttttaatac taaaacttat atagataata tttttgccat
     4561 ctcataagct tctttatcaa atactttctt ttgagctaaa gcttcactta tttctaaatc
     4621 aattattttg ttttctttta ttcctacaac tctagctgat tttccatcta ataataattc
     4681 tactgctctt actccaagtc tacttgctag tattctatca gatacagttg ggcttcctcc
     4741 tctttggaca tgccccaata cagtgactct taaatctgct cctgactctt ttttaagttc
     4801 cttttctaaa tcactagcac ttccgactcc ttctgctaaa acgattatac tatgtctctt
     4861 acctcttttt tgagtagtct ttaatcttag agcaacttca tctactgtaa tttctacttc
     4921 tggtactatt atagtttccg ctcctcctgc aagacctgca tataaggcta aatctccaca
     4981 gtgtcttccc ataacttcta ctatgttaac tctttcatga gatgatgatg tatctcttat
     5041 cttacctata gcatctataa tagtattcat tgcagtatca aatcctatag tataatctgt
     5101 atatgctaaa tcgttatcta tagttcctgg tatgcctata gctgggaatc ctaagtcact
     5161 aagtttttgg gctcctgcaa atgaaccatc tccaccaatt acaactaagc aatcaatttt
     5221 atacttttta agaattttta ctgcttttaa tctaccttct tctgttttaa attcttcaca
     5281 tctggaagat tttaatatag ttcctcccct gtgtataata tctccaacag aagataagtt
     5341 catttcagtt aaatcttctt ctagtaatcc cttatatcct ctattgattc catatacttt
     5401 gcatccataa tatattgctg atcttacaac cgctctaatc gcagcattca ttcctggagc
     5461 atctcctcca cttgttagta atcctatagt tttcattttt tacctccttg gattttatgt
     5521 caatttttta ttataatcat tgttattgta ttctaatgga ctttttttgt ccctatatca
     5581 ctttttaatc ccatcaagac taaaaaaatt aaatgtcctt aaatgtccat tttgtgatat
     5641 tatatcacaa aacggacaat atttttcata ttattataat atatttagat ttttatataa
     5701 aaaatcaagc ttttactttt atgttatctt taggaagtaa ctcttctaag ttatttataa
     5761 attcatcaca tatacaagtc aatgccccgg tcattttata tgctttacgt ttaccattta
     5821 tattttcatc catagggtat aagtatatac tgtcattacc tggatattgt ttggctattt
     5881 ctacaatatt attagttgta tcagtatcag acatactatc tattttcaaa tacaattttt
     5941 taccactttc tggcttaata ttttctacat ttctagttct tcctgaattg tagtttctat
     6001 ctgcaaagtc agtatcatca tttatatctt ttatctctct agctataagt tttgcatttt
     6061 cgccttcttt tatacttaat gttcctttta catatattat attatcttca ttcaaaatga
     6121 cattatactt ttgtaataat tgaggaaaaa ctattatttc tatagcacca tacaaatctt
     6181 ctaattcaat aaatgccata atttcatttc gttttgttgt tttaatagtt ttagttacaa
     6241 tcattccacc cattataaca tctgtttcat tcatttgtag gtatgactct tcttcctctt
     6301 ttaatgaatt taattttcca ttatccatag atgttttttc ttgtagttct ttttcaaatt
     6361 gtgataaagg atgtccactt acatacattc ccaagacttc tttttcaagg ttaagtctct
     6421 ctctttcttc gtactcttgc cttttaggca aactgtatat ttgttgaaaa tcactatctt
     6481 cctcactatc tccaaaagca tcaaataaag aaatttgacc ttgaacattt ttctttctat
     6541 ccatagatac actttctaat aaactttcat aaccagccat cagactagct ctattttcac
     6601 ttatattatc aaaagctcca cattttatta gactttctat tactctctta tttgtatctt
     6661 tttggtctaa tcttttgact aaatctacta aatctacaaa taatccatta gattctcttt
     6721 cagcgattat attgtttatt atatttacgc caacattttt tacggctgct agaccaaatc
     6781 ttatattgtc gccttcaact gaaaactttg aaaaactttt gtttatatct ggaggtaata
     6841 catctatttc tagtgcttta cattctctta tatactcaac taccttatca gtatttccca
     6901 ttacactggt tatcaatgct gccataaatt caactggata atatgttttt agataagctg
     6961 tctcatatgc taaaacccca tatgcagctg catgtgattt attaaatgca tatctagcga
     7021 agtctatcat atcatcaaat attttattag ctatttcctc ttctacacca tttctgatac
     7081 accctgcaat ttctatatta ccatcttcat caaatttacc atgaataaaa tattgtcttt
     7141 cttcttccat cacgtccatc tttttcttac tcatggctct tctaactagg tcacttcgtc
     7201 cgtaactata accacctaaa tctctaacga cctgcataac ctgctcttga tataccaaac
     7261 aaccatatgt aacttccatt ataggcttta atttttcatg tagatatgtt acatcttttg
     7321 ggttgctttt attttttata tatgcaggta tagaatccat tggacctggt ctaaataggg
     7381 atattcccgc tacaatatct tcaaaattgt ctggttttag ctgtttcata aaacttctca
     7441 tgccagcact ctccaattga aacaccccta gtgtatttcc tgatgaaagc atttcatata
     7501 ctttcgggtc gtcataatcc atctttgaaa agtctatttt ttctgtgtaa ttttttataa
     7561 ctctattttt ttctattaaa tcaagagtat ctcttataac tgtaagagtt cttagcccaa
     7621 gaaaatccat ctttaaaagc ccaagttctt caagtgttgt catagtaaac tgtgttgtga
     7681 ttgcatcttg atgtttgtac aaaggtacat attcatctac gggatttttt gatataacaa
     7741 ctcctgctgc atgagtagaa gcatgtctta gcattccctc tatctgtttt gatatattta
     7801 ttatttcttt tgtttccggg tcttggtcat acaattcttt aagattagga tttgtatcta
     7861 gggccttatc tattgtcatt cctaaagcaa atggtatttc cttggctacc ttgtcaactt
     7921 tattgtagcc tatatccaat acccttccaa catctcttat tgcagctttt gcacccattg
     7981 ttccaaaagt tattatctgg gctacatggt catcaccata ttttctcttt acataatcta
     8041 taacttcttc tcttctctca taacaaaaat ctatatctat atctggcatg gatatacgtt
     8101 ctggatttaa aaaacgctca aataatagag agtactttat tgggtctata tcagttattt
     8161 taagtgtata tgctactatc gaaccagccg cactacctct acctggtcct actataatat
     8221 tattttcttt agaaaaattt ataaaatccc aaacaattaa aaaatactct acataaccca
     8281 ttttttcaat tacatctaat tcataattta atctctctag tatctcatta ctaggattat
     8341 catatctctc ttttaatcca ttaaaacaca actcccttaa ataagtttct ggtgtatatc
     8401 cttctggaac atcatactta ggaagatgta tagtattaaa atcaaactcc acattacaca
     8461 tatcagctat tttcacagta ttgtcaatag cctctaatgc atatggaaat aattcttcca
     8521 tttcttctct tgattttaaa taaaactcat cactaccaaa tctcattcta tttgggtcat
     8581 ttaatgtttt tcccatttga atacacataa gtacatcatg tattttcgaa tcttctttat
     8641 ttacatagtg aacatcatta gtagcaacta gtggtatacc agtctctttt gataatttta
     8701 ctaaaccaga gtttacttct ttttgctcag gtaaattgtg atcttgaatc tccaaaaaga
     8761 aattttcttc tccaaatatg tctctgtagt caagagcaaa ttgttttgct ttttcataat
     8821 ttctattcat taaagcacgt gctacatctc ctgcaaggca tgcagaaagt gctataatcc
     8881 ctttgttatg ttttttaagt tcttccatgt caactctagg cttatagtaa aaaccttcta
     8941 cgtatgaagt tgaaactatt tttattaaat ttttatatcc ttccatattc ttagctaaca
     9001 gaactaaatg tccttgatat ttgtcatagt ttgggtcttt atctctcaat ccacgtgcag
     9061 ctgtatagac ttcacaccct ataattggct ttattccttc ttttatagct gttttgtaga
     9121 aatctataac accaaacata caaccatggt cagttatagc tattgagctc atgtttagtt
     9181 ccttagccct ttttataagt tttttcaccc tcgaaaaccc atcaagtaaa ctatactcag
     9241 tatgtacatg aagatgagca aaatctttgt tcattaaatc acctcctgct ttataatttt
     9301 aattttacca caaatatatg tcctaaaaca aaaacagaga cttttatctc aaaataggaa
     9361 ttaacttcaa cgtataccat tatatgttat aaattaatgc ttattctgag attgtcccct
     9421 caatttattg ctatgatttt tttttcaaat ctgttgcaat ttcttctatt tttctaagtc
     9481 tatgattgac tccagatttt cctattggtg gttctaacat ctctccaagt tctttgagag
     9541 tcatatcctc atactcgatt ctaatcttag ctatctcttg taaattttct ggcaaactgc
     9601 taattcctat agtctcttgt atgagcttaa tattttctac ttgtcttact gctgcattta
     9661 ctgttttaga aaggttagct gtctcacaat ttactatcct gtttacatta tttctcattt
     9721 cttttactat tttagtattt tgaagactta aaagagcgtg gtgtcctcct ataatactca
     9781 acaaatctga tatttgttca ctctctttga tataaacaac gtgattattt tttctagcca
     9841 caattttact attaaaacct aaagagttta taagttcttt tagagaatct gcaaactcct
     9901 cattattagt tacaaattcc atatgatagt tcttttctgg gtcgcttata gaacctccac
     9961 caagaaaagc tcctcttatg aaagctctct tacatccttc atgcttaatt aagttctctg
    10021 aaattttgtt tatagtaaaa aatccttctt tatcttcaag tatacctaac ttttttaata
    10081 acatttctga acccatttcg cttgttacca ttaatacata actgttattt ctctttagca
    10141 tttgattctt atttactgag attgtagtat tgatattaaa attctttttt aataatttaa
    10201 atactttacg ggctattgaa tttaactctg ttgttatctt tagatttagc ttcttatatc
    10261 caacaatttg tatacttccc cctgatttaa ctagagctga caattcagct atattacaac
    10321 attcattccc agatataact cttgctagtt catttttggt ttcagttgaa aaagacatat
    10381 ctatcaccta ttaactaatt tttctttaac ctttttttta tttcttgtaa ggcaaccttt
    10441 attatctcct tttcatcatc atacctagct aagtctacta ctctatctag gtggataaat
    10501 ttagcatcaa taaagccttc ctctttttgt ggaatagtat ccatattctt tgcttgcatc
    10561 aaataccaaa aaactgtttt atgaactgct ctattttcat cccagttttc tttgaatgta
    10621 tagtgtattt cacctaaata tttcaatata tctgctttta ctccagtttc ttcacttact
    10681 tcacgtaatg ctgcctcttg gttattttcc ccttcttcta cttttccctt gggtaacacc
    10741 caatctccat tgaacttcct tagtaaaaga atcgtattgc caaacaggac tacaccacca
    10801 gcactaacct cttctctcat cgatgttcac tccttaaaac taacataatg ataaggattt
    10861 ctacacaatt ttttaaaatc cttttttact aacttttgtt gtaattatga tttagggcta
    10921 actcaataac tatgttagat atatactttg catcatgtct tatataatta ttttttattt
    10981 caattaaatt cttctctaca gtttttatac ccatctcttt taatttatct ttttgctttt
    11041 tatctaacaa gacttgctct gctccatctt gtttatataa gtcaagcatt tcttctggca
    11101 atatctcatt atttgcgatt acataatcta ttaaattctc atcagtgtgt tttactatag
    11161 cattaacatg ctctaataca ttgtaccctt ctgtctcacc tggttgagtc attatatttg
    11221 ctatatatac ttttggagct acagacgatt taatagcgtc tacaatcccc tctacaagta
    11281 aatttggtat tatacttgta tataaacttc caggacccat aattataatg tcagcatcat
    11341 aaatagatgc aataacatcc tttaatggtt ttacatcttt tggatttaaa aaaattttat
    11401 caatagagct tttttgaatt tttgactctt caggtatact agattctcca tttattatat
    11461 tgccattttc taatttggca actaaattta catcttccaa agttactggt aaaactcttc
    11521 ctgttatagc aaaaatttca cttaatttat atacagcttt ttcaaagttt ccatacaacc
    11581 cattcatagc agccaaaaac aaatttccaa aactctgacc ttttaaaagc ccttctgtaa
    11641 acctatattg cataacttca ttcattgttg gctctatatt tgcaagtgct aataaacagt
    11701 ttcttatgtc tcctggtggg agcattccta agtcttctct caatacacct gaacctccac
    11761 catcatcagc aacagttaca attgcagtta tattttgcgt agtgtgttta agacctctta
    11821 aaaataccga ttgaccagta cctccaccta tcacaaccac atttggttct ctattttcaa
    11881 caatagacct ttgaaatagt tttacttttt cattttttgc ttttttatat ataaataacg
    11941 atacaattaa tgcaatccaa cctaaaattc ctacaataat aagtaattta gccatgtttg
    12001 cctccattat cgcaacatag agtctctatg tttctttaca actctatatc ccttattaga
    12061 taaatcctcc gctataagat ttgttatagt aactgaccta tgtcttccac ctgtgcatcc
    12121 aacaccaatt actagatgtt gttttccttc ctctatatat tgaggtacta aaaaatgaat
    12181 catatctaaa agtttcacat aaaattcttc actgatttta gaattcataa cataatctct
    12241 aacttcttta tcatcaccag ttttagctct taactcttca acataatagg gatttggaag
    12301 aaatcttaca tcaaatacta aatctgcatc agcaagtata ccatgtttaa atccaaaaga
    12361 tactacagaa atagttaggt ttggattatc ttcaccagaa gaatatattt tctttatttc
    12421 ttccttaagg tctttaggtt tcatgttagt tgtatcaatt atacatgtag ataactcctt
    12481 taaaggttcc attatctttc tttccatttt tataccttct ggaatctgca tatcttttgc
    12541 caatggatga tttcttcttg tcattttata tctctttagc aatacatcat cattacaatc
    12601 caaatatacc atttcatatt cataattttc tttttctaaa taatttaagc tttcatgaag
    12661 cgcttcaaaa aactttctcc ctcttatatc tatccctaga gcaactttat caatacttga
    12721 atttggttga taacataatt ctgcaaattt agttatcaaa gcaggaggta gattatctac
    12781 acaataaaat cccatatcct ctaaagctct cattgtctca ctttttccag atcctgaaag
    12841 acctgtaact ataacaaatt tcattgctaa ctcctccctt ttatcttatg tttattatat
    12901 tataatgttt tctactaacg atatatccag attagagtca gatatagtct tcatagctag
    12961 ttcaaaatca ccaactcttt ctggacaatg tgcatctgag cctattataa atttattttc
    13021 tagccctttt attttattta attgatttac attcaaaaaa gaatggctac tatttatctc
    13081 aagtctagta tttgtctttt ttgctgtctt tgctacttct tctatataca catcgccttt
    13141 atctcctgga tgagtaatta taaaaatatc attattactt atcgcattta ttacagcttt
    13201 tgtattatat tcttttgctt tttcagtttt aaaaatatag ttattacaat gatttaataa
    13261 gcttcttaat gatgttggag tagaaccaaa atgatatcca gccataatat aatcaaaatc
    13321 tctaataaat ttatcatcca tatctatatt tcctttatca tctaatatgt tacactccat
    13381 acctaaaagt atatttatat cagtgtactt atcatttaaa taatctactt cttctctcat
    13441 tttgagaata tcttttttct ttacaccata tgcaatatgt ttataactat ggtcagatat
    13501 tcctattgtt tttattcctt tagaaatggc tacctttaca ttatcctcaa tagtcccttt
    13561 gccatgacta tataatgtat gtgtatgata atcagccagt attttcattt gttaatcctc
    13621 tccaagtatt ttgacttctt cttccaacat aaccccaaac ttggcatata cagcactttt
    13681 tataacatat attaaatcca aaatatcttt agcacttgct tctccttgat tgactacaaa
    13741 tccacaatgc ttctcagata cttgtgctcc tcttaaagta agacctctca atccagtttc
    13801 ttctattaat ttacctgcaa aatatcctgt tggtctctta aatgtacttc ctgcactagc
    13861 aaaatttaat ggctgtttag ttattcttct cgtcgctaaa tcatccatta aactttttat
    13921 ttcatcataa tttccttctt gtaattctaa ttctgctgat aaagctatat atccgttttt
    13981 cgatattata ctttttctat acccaaattc catctgttca tttgataatt caaatatatt
    14041 gccttccata tccatcagtc ttactgactt cactatatcc ttcatttcac caccatatgc
    14101 tccagcattc atggctaatg ctcctcccaa tgtgccaggt attcctgatg caaactcaaa
    14161 cccttttaat tcttgctttt ggagagcttt ccctaaaact gataataaag ctcctgactg
    14221 agcagtcatc ctagtatcat ttatctcata agaattgaaa ttatcagcta attctattac
    14281 cactcctctt attccaccat ctttaattaa aatgtttgaa ccatttccaa ttattaaata
    14341 cggaatagac tcttttttta ccaacttaag tacatttttt aattgttctt cagttctagg
    14401 tctaactaat atatctgctg gtcctcctac cctaaatgaa atatgttttt tcataggttc
    14461 atcgactttt atatcctctt tacttaatat atttaataaa tttatatata tatcttgatt
    14521 attcattgta tttcctccca aattatactg aactttagct gtcattatta tttaaattac
    14581 attaatatat agtattaact atattcaata atatatgatt ttgcttatca tatggatttt
    14641 atattttaag cttcttaatt acaaactata ttcaataaat atataataaa gtaaaaaaaa
    14701 aaaaataaca acatataaaa gaatataaat aatttaaaaa aagcatcaat atcactgata
    14761 tcaatgcttt aaaaggcaat ttaattcgta ttatttatct aactattttt gacatagcaa
    14821 agataataaa tccctccaac tactatggaa cctccaacga tatttccaag tgttacaaat
    14881 accaaattag ttataaattg aaccatagtt atatttgctc ctaatgccat tcccattggt
    14941 ataaaaaaca tgtttgcaac actatgttgg aatccacata atacaaatag cattattact
    15001 gtccaacatg aagccaattt tgacattata tcttgagatg ctgtagccat ccatacagct
    15061 aaaacgacta gaatattaca aagtattgct tttaaaaaca ttgatgctat agttgaatta
    15121 accttagctt ctgccacagc tatagctttt gtagctgcat ctcctgtcaa tgtagaagaa
    15181 taaaacacaa gtgcaactag caaaattgaa cctactaaat ttgcaaaata aaccagtccc
    15241 cagtttctta acatttttcc taatgttatt tttttgtcca taagtgcaat agccattaaa
    15301 gtgtttccag tgaataattc tgctcctgcc actactacaa gcataagacc tactggaaag
    15361 accatagcac ttaaaaagct agctacccct gggtctatct tgccaaaagt ttgactaact
    15421 atcatactcc ccaatgcccc aaaacctata aatgctcctc caaataaacc taatagaatt
    15481 gcattttgtg tcgaaatatt tgctttttta atgcctgcac ttatagttgc ttgtgcagtt
    15541 tccccaggtg tcaagaatcc tttactcata aatccttccc ccttaaaaat tatactttcc
    15601 cttgtctttt tatggcaata attatataat aacaatatga tatataataa attttattaa
    15661 cataa
//
LOCUS       DAELTA010000095        15293 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.095, whole genome shotgun sequence.
ACCESSION   DAELTA010000095 DAELTA010000000
VERSION     DAELTA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.095"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..399)
                     /locus_tag="KPG13_002433"
     CDS             complement(<1..399)
                     /locus_tag="KPG13_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG0986143.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRN"
     gene            complement(416..1222)
                     /locus_tag="KPG13_002434"
     CDS             complement(416..1222)
                     /locus_tag="KPG13_002434"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG0986144.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(1424..1909)
                     /locus_tag="KPG13_002435"
     CDS             complement(1424..1909)
                     /locus_tag="KPG13_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG0986145.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(1960..2619)
                     /gene="spoIIR"
                     /locus_tag="KPG13_002436"
     CDS             complement(1960..2619)
                     /gene="spoIIR"
                     /locus_tag="KPG13_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG0986146.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(2764..3444)
                     /locus_tag="KPG13_002437"
     CDS             complement(2764..3444)
                     /locus_tag="KPG13_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986147.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(3428..4318)
                     /locus_tag="KPG13_002438"
     CDS             complement(3428..4318)
                     /locus_tag="KPG13_002438"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG0986148.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(4436..5986)
                     /locus_tag="KPG13_002439"
     CDS             complement(4436..5986)
                     /locus_tag="KPG13_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG0986149.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(6080..6346)
                     /locus_tag="KPG13_002440"
     CDS             complement(6080..6346)
                     /locus_tag="KPG13_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG0986150.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(6494..7354)
                     /gene="yabG"
                     /locus_tag="KPG13_002441"
     CDS             complement(6494..7354)
                     /gene="yabG"
                     /locus_tag="KPG13_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG0986151.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(7438..8376)
                     /locus_tag="KPG13_002442"
     CDS             complement(7438..8376)
                     /locus_tag="KPG13_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG0986152.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(8486..8647)
                     /locus_tag="KPG13_002443"
     CDS             complement(8486..8647)
                     /locus_tag="KPG13_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986153.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(8657..9154)
                     /locus_tag="KPG13_002444"
     CDS             complement(8657..9154)
                     /locus_tag="KPG13_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG0986154.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(9269..9979)
                     /locus_tag="KPG13_002445"
     CDS             complement(9269..9979)
                     /locus_tag="KPG13_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG0986155.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(10155..10685)
                     /locus_tag="KPG13_002446"
     CDS             complement(10155..10685)
                     /locus_tag="KPG13_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986156.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(10702..10932)
                     /locus_tag="KPG13_002447"
     CDS             complement(10702..10932)
                     /locus_tag="KPG13_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986157.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(10947..12341)
                     /locus_tag="KPG13_002448"
     CDS             complement(10947..12341)
                     /locus_tag="KPG13_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0986158.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(12467..13720)
                     /locus_tag="KPG13_002449"
     CDS             complement(12467..13720)
                     /locus_tag="KPG13_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0986159.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(13771..14925)
                     /locus_tag="KPG13_002450"
     CDS             complement(13771..14925)
                     /locus_tag="KPG13_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0986160.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
BASE COUNT         4784 a         2877 c         1491 g         6141 t
ORIGIN      
        1 attcctacct aaaaataaat cttgtacctt tatattatag tctattttca attttatgtg
       61 ttccccttct tgtatattta tatctaattc tcttgtatca atatcaatta ataaagtacc
      121 ttgtggggtc acatactttg tagtactact ttctccacat ttaaacagca ttgttgagtt
      181 aatagcacca aactttttga tactaacttg atttttaagt atctttatag tattagttat
      241 ttctatatct tcttcttttt ccttgtaaat aacatatatt ccatcatttt tataaaaaat
      301 tttaccaaag gcagtcatct ctatggtgtc catatttcct ttttcatcat attgtctagt
      361 atttatactt atttttacac tcaacgattt ttcctccata gattcttcat ataaactagt
      421 atttttgtat ttctactttt tgaatcttat caattttttt ctttaagaac tctcttgcta
      481 tatctgaaaa tttttcaggt atatctgata cataatattg ataagttcca tgaatttctg
      541 tattgtttat aatattttgc acctctaaaa tcttttttaa atcctttgca gtctctttag
      601 ctggattaac aagttttata tgctctccaa cttcttcacc tattgttctc tttaaaatag
      661 gataatgagt acatcctaac actaaagaat ctatattttt ttctttcaat tcttgtaaat
      721 atatctttgc tgtcagttta gccacttctg tatttgccca tccttcttca acaataggca
      781 caaataaagg acatgcttta ttaacaattt ctatactctc atctatttta gatatttcca
      841 agttgtatgc ttttgaactt atagttcctt cagttcctat tattcctaca attttgtttt
      901 ttgtagaact tacagctgtt ctagctcctg cttctataac tccaattatc ggtatatcat
      961 atttttcttg agcttctttt aagcttcttg cagtagcagt gttacatgct ataactatag
     1021 cttttacacc ttttgttttt aaaaagttta tagcttgaaa agtatattta attatagttt
     1081 ctttggatct tgaaccatat ggtattctgg cagtatctcc aaagtatatt atatcctcat
     1141 ttggtaatat cttcattatc tcttttagaa ctgttaatcc acctaatccc gaatcaaaaa
     1201 ctcctattgg tttattgttc atctttatac aacacctact ttacattttt gtgtttaaca
     1261 acaacttatt tatcgctaaa atttatattt atattcataa ttaatatatt attacctaaa
     1321 tgattattat agtttatttt acacaaaaaa taaaggagct atcttaaatt agaaaataat
     1381 ttctacaaaa agacaactcc tttcactact taaaaattta aatttatttt gcaaacacat
     1441 gatttcctat tctcatataa ggattcaatc tatttatcca tttgcaagtg gctatttctg
     1501 gattccaaaa atatactgct ttattagttg ggtcagttcc ataaagagct tcctgagctg
     1561 ctttacgtgc tgagtctgtt ggtgccatct ttattgaacc atcaagcaca actgagaaag
     1621 catttttttg atatatgact tcttctattg tgtctggaaa tctagggtcc aatactctat
     1681 ttattactac agctgctaca gcaacctgtc cctcatagct ttctcctcta gcttctcctg
     1741 caaccaactt agataataac atcaagtcat tatcagataa atttattact tctttctttg
     1801 ctatattttt ttcttgtttg tttactttct taatattttc tgttggttca tatgcaaata
     1861 cctctttaat aggcataaat ataaaagtca aagctaatag tgtagccatc agtttactcc
     1921 acctgtccat gattaaaacc actccttaca ttcttatttt taaaattttt tctctaaatc
     1981 cttgactact tcaagtactt taaacttaat ttttactcta cttgtttttt ttggtgtatc
     2041 ttgttttatt aacttatatt ctttatcagt taaaacttcc ttcaacttat catctgtaga
     2101 tttatctatc actccattag attcatcaac aaaacatagt ggagggaaca taacacacca
     2161 ccagtttttt ccttcacctt tcccaataat aatctttaat gctttatatt caccagctgg
     2221 aagaactata ttagaatatt gtttagtagg gaaattgcta tattgtatac ctacctttac
     2281 actgtaatta tatccatttt taagaataat atttttactt atttcttcca gactactata
     2341 ttcctttttt ataatagctt cactttcttt tatacttttt gaattttgaa gctttggctg
     2401 taaatatttt attacttcat ctctaacttt tagtttaagt tcttggtctt catctgtatt
     2461 gctgtttgct ataacatgaa atcttattaa tttatcttta taatcttttg atatattgtc
     2521 tacttttttt acctcaccat ttattactat tgtcattatt gatatgactg atataagact
     2581 taatattaat agtcccaatc taatcttaat ttttctcatt gaaaattccc cctcaagata
     2641 aatttatatc atcaattatg tccaattaaa tttaatttat tcataatatt ttttaatact
     2701 aataaaaaaa gctatcttaa aatgattaca atcattttaa aatagctttt taaaccttat
     2761 attttataag tctttactaa tcatagcctt atctatcata aactgctctg ctttttctat
     2821 atgctctgta gcatacttct tggatagctc actatcacct ttaattatag catcaagtaa
     2881 atatttatgc tcttcaacaa tattatgtga tgtatcataa gcagacatat aggtaactct
     2941 aaatctatat acttgttccc acaatgagtt tattaattgg attaattttt tattattagt
     3001 tgctttaaat atacattcat gaaattctac atcaagtttt aataacgcat ctacatcgga
     3061 ttccaaagtg ctttttttaa attcttctga tatatcttta agctttttaa tatcttcatc
     3121 actaattctt tcagcggcaa gatatgctgc taagccttct aggcttgctc ttacttctaa
     3181 tacatccata atatccttta atgacatatt tgccacatac gcaccttttc taggcaacat
     3241 aacgactaaa ccttctaatt caagctttct tattgcctct ctcactggag tcctacttac
     3301 tcctaattgt tctgctagtt gcacttccat tagcctttga ccaggcttta attttccttc
     3361 taagattgct tctcttaaat tctcaaagac tacatctctt aatggcttgt aattatccaa
     3421 gttcagttta gttaaattct ccacaaattt caaccccttt ttgactgcta ttaacaacat
     3481 acacctgttt atattttttt aataattctt tttttccaat tagagcatct tctttatttt
     3541 taaatagtcc aaaaactgta ggaccacttc cactcatcat agaacctaaa gcattatttt
     3601 tcatcattac ttgttttata tcacttattt ctttgtgttt acctatagta acattttcaa
     3661 gaatatttac catgctttct gatacagctt ttatatcttc agattttaaa cactctatca
     3721 aatatttatt atttggtctt tttttaatat tattcaaatc taatccttga taaacttctt
     3781 tagtagaaac gaataagtct ggtttgcata tcaatatatt tgtatcacat ggtaaacctt
     3841 tgatatttgt tagcttttct ccaatacctt gagctaaggc aggtcttcct gatatacaaa
     3901 aaggaacatc tgcgcccaaa ttaagtccta tttcttttaa ttcatcttca gataatctaa
     3961 gttcccatag gtgatttaaa cctactaata ctgcggctgc attagaactt cctccagcca
     4021 tccctgctgc tacaggaata tttttttcta tgaatatttc tacacctttt tttatataaa
     4081 atttattttt aagtattttg gcagctttat aaacaatatt atcttcatct aaaggaatat
     4141 ctaaactagt gctttttacc tttatttcat cttcgtctaa ttcttttatc tttactatat
     4201 cgtataaatc tatcgtttgc attatcattt caacaaaatg ataaccgtct tgcctttttc
     4261 ccaagacatc aatggataaa tttatttttg ctctactctt tagtcttata aaatccattc
     4321 tatcctcctt gtttaaagaa taatgcatca ctaacaataa tattatacta caaaaaataa
     4381 aataataggg tattataata ccctattata cttaattact ttaaattttt taatattaat
     4441 ctactaatac gacttttttt tctaaaataa gacacttccc aggcttaatt ggttcatcaa
     4501 gttttatatc attaagttct gcaatttcat tttctgttgt attgtatttt tttgctatat
     4561 tccagaaagt atctccttct ttgcatatat atacgattat gctaggagct ttagataaat
     4621 catatacacc ttggtcttct cccttgataa taaaattttc tgctttctta tctaatgctt
     4681 cagtaaatct cttaattttg ataatcaagt ctatctggtc cctattcaaa tcaacttcga
     4741 ctttatcaat acatgctgta ttaaacacag atgctgtatc agttagatta tccatttcta
     4801 tatcatgttc aaatggaatt tcctcactaa ttttataaac caattttaaa ccttcaactg
     4861 ggacaaataa aatttcaact tttatgattc cttgtataat gcttttattt ccttcaatat
     4921 aactattttc tatagacatg gttggacaaa cgcttacaat atcttttatt tgtatatcat
     4981 cattatcatt tcttatgcct tctctaacca taaatgtttc ttcactacgt cttaaagtct
     5041 tatttaattg aataggtttg tggtcaaatt ttattatctt ttgaggcgaa tatgcatctt
     5101 gtaatacttc tcttgtaact tcatctgtaa cttttacctt acaacaagcc atacaatcta
     5161 tttctaaaag acctgtgttg ctttcactat tttgcttgaa aatatgatta aaatctgaca
     5221 tagacaacaa tacttcttca gtcataccat cactaactcc agggacttct acaaattgag
     5281 taaactcaat acctactcta tccaactcta caagttcacc ctcatatgta caagctaacg
     5341 gatttatttc taatactcca ccaataataa ctttattgtc agtcactcta ctttctttta
     5401 ctttaacaca tggatttaaa cttattatag attgaatttc ctctgtgttt attgttatag
     5461 tatctcttat agaactttct gctttttcta ttccaactat atcttgaaaa caaatttcct
     5521 ttctgtgctt ttgtatgcat tccacttgcg ccacatcttt tacaatgtct aacctttgct
     5581 tttcaaacaa acttcccctt atattcataa gagctcctac tttaatcttt ctctcattca
     5641 ttatagtaca atccatatgc tctacttctg aaaataacat gtattccata tcttgaataa
     5701 cattgtcttt ttctattact tcgtttatat ctacttttcc gtctacatta gaaatagtat
     5761 ttttatcatc tgcaatatag attacattat aattaaaact acctctgcaa agtatcttcc
     5821 catctgccat ttcaattttt ttaagtgata tgtatccttc tgtttttaca atttcatata
     5881 catctaattt tttgtctggc acaactgcct ccgcttcaat gaatgtttga aattttccaa
     5941 agtctattct attgtcaact ttaattacat ctttaattaa ttccatagtc tacctcctta
     6001 agtattataa aaatatcaca ctatatttat atttaaggag caaaaaaaaa attacaccta
     6061 tttttaaata ggtataattt taacttattt gtaatttatc ttgatctcta aaaacttgca
     6121 actttacatt agatgttaat aaatctgagt aactataaga tactctagga tatccattac
     6181 tttcatcatc taactttata acaaacacgc ttggataaac tttttctagt ataccctctt
     6241 ttgtaataat ctgctttctt cctttattag ctttcaacaa tattttcttt cctatatgtc
     6301 tctctaagct tactcttatc ttatctagag tttgaacagt agccacaata tcaccttctt
     6361 taacgtttat atgtttacat aatatcacat ataaacattt ttgtcaaatg tttaaactaa
     6421 ttattttata tattttttgc aaaaatgtca atactatttt tgaagatata cagtataatt
     6481 atgttaattt ttgctaatgt aatattgttt ttggcattgc ccatctaaat tttcctcttg
     6541 tttcagaacc accaattccc ttaacacctg taatagtctg ctctataacc tcatcaatag
     6601 gaactactac atcaattctt gaacaagcaa ttttctccac tataaaaaca gggtctaatg
     6661 catgtatcat tattcttcca ggcgaacttg catagtttgc tcctgcactt attatttttt
     6721 cataatttga ttggcatgct cctgcgaata tgacaagatt atccaaattt ggttgccatt
     6781 tacgtgcttc ttttactgtt ttaacaaaat ttgaagagtt tctatagtta ctcatatctt
     6841 ctatatctcc tctttttatt gtcattgcat catgacctgt tattactagt atatccggat
     6901 tatatttttc tagtagtgct cttacttctt tatactgatt ttgctctgaa acagctacac
     6961 ctactgcagg tattccaagt tgagcatata catccaagca aatttttaaa tattctttat
     7021 ctccatctat ctgtagtact tttccaggca ttccatatgt gttaggattt aattgaagtt
     7081 ttggaactgc tctagtcatt cttttttgtc tttctttagc ttttcttact gacttatata
     7141 ataaattttc tacattttta tctattaata tatcttttat atcaggtgct tttactaatt
     7201 ctaggtcatc aatataagca tctgctatta ctctaaatgc aattcccttt agtatagcta
     7261 ttttctcatt attttcatct acaccaaaag agactatttt aaatactata tctttgttat
     7321 gtgactttct agctacaatg tctcctacct tcatagttaa acctccaata aatttttatc
     7381 ttagtttata ctatgttcga tataaaactt tgtttcctaa tgtcaaaaaa acatttctta
     7441 taatacttct tttatcactc ttaaagcatt tttatagtat atcttttcta tatcatcttc
     7501 actaaaccct tcttttttta gaggttcata cagtttaccc atctgagata tatcttcaat
     7561 ctcaactttt gaatctattc catcaaaatc tgaccctaga gatacaacat ctattcctcc
     7621 aacatttaca atatgtttta tgtgtctaac catatcttca agtttacttt cacttttatc
     7681 acttaaaaat aagtggaaga agtttatacc tgtaactcca cctttatttg ctaatacttt
     7741 aatcatatca tcagttaaat ttctagaatg attcatcatt gctcttgaat tagaatgtgt
     7801 agcaattatt ggtttagaag aaatctttgc aatttcataa aatccaccat ctgaaatatg
     7861 agaaacatca accaacattc ccaattcatt catcttatgt accacttctc ttccaaaatc
     7921 agtaagccct ttttctctat attctacttt attatgagga aagctcaatt cattcacatg
     7981 attccaactt attgtcatca ttctaacccc taagtcgtaa aatttcttta aattttctaa
     8041 tttaccttca agtactgcac cttcctcaat agataaaaga gctgtaagtt taccttctga
     8101 ttggtttttc ataatttctt catagttagt agctaaagct atttcatcac tatttttttt
     8161 catttcctca tgaaatttat ttgccatact catgcaataa tcaaaaggat gttttacttc
     8221 ttcagtatca acaaaaagag caaatgtttg agctaatgaa tccccctttt tcaatctgtc
     8281 tatatcaaca gaatatttat tagacttaag ttcacttgtt tctacatttt ccattaattt
     8341 tgctatagta tcacaatgta aatcaagaaa tttcatattt ataccccctg tatattttat
     8401 atttatatct aaaaatagaa atagttgtcc ttatagacaa ctattgacaa ctatttttta
     8461 atattctttt taatattact taatcttatt cttcttttaa gttaggtatt acttttttaa
     8521 cttcttcaat ttgatgccat gttttatcta tattattttc actaatatat ttttttaaat
     8581 cagttaaaga tgtaaaatct ttaactgata aattgtcatc atcatctgca aatcttaaaa
     8641 taaacatgag actcccttac tttccacaac atttcttata tttttttccg cttccacatg
     8701 ggcatgaatc atttcttcct atttttgctt ctttaactat tgttttagac ttcttataag
     8761 cctttattat ttcttttctt tggtctgctg gtagtatatc ttcccaacct tgtaaattat
     8821 atagccaatg agcctcaaca gttaccatat tgttatataa tttttcccaa tctattttta
     8881 tagaaacttc tttatcagct tctacttctt ctaaaactat atcttctttt aagctttcac
     8941 ttattccatc tatgaatccc atgaaaaatt cattagtagt tccaaatttt tcagccaatt
     9001 cacttacttt accttcaaca acatccattt tattatttaa tatttcattg tatattccag
     9061 cttcaacttt caaatattct tcccagaatt ttacttctgc ttcttgactt tcatgattat
     9121 cacttaaatt tctccattca gtatataaac tcatactttt tcctcctaag tatacctata
     9181 tttttatagt ttttatagta taaaatcttt tagttagatt ggaagtttct tctttttgat
     9241 tataccatat tctatttaaa gttaactctt atattatatc ataattaaac aattctttta
     9301 atacttttat agcatactct tggtttataa aataagggct tgtcgcaatt gttattactt
     9361 tggctttttt aattaagttt tttatcttgt aaaatttgag ttcaaatggt atatagtcca
     9421 tatcacttga aaatatatcc aaatctatat caagaacaaa ctctgattct acttctaaat
     9481 caaatccata tagactatct attattatta attctgaaaa tatgttatat tttaaagcag
     9541 gttttataaa acttcccaca tttaaaactt catttgtata tctaaataca tcttttagat
     9601 tatttacatc tacatcatag ttctcaggtt ctctagtatc cttatgttgg tctacatgca
     9661 ctaacttgca tccttttgta aactcaccct ttaacaaaga ttttgcccaa aaataaaatg
     9721 catggttatg attgtcaaac acatatatct ttttatttcc caggacatat tcgaccatat
     9781 tctccaatcc ttttgctttt atttccttat tttcatctac ttcattaaat actacctcat
     9841 tgcctaacct tacatcattt aatgtaccat ttattagttt tggaacgtat atgctcttat
     9901 tttctctttt atcatatgaa aatacattat ttcctactgg tttttctata taaaatccac
     9961 tatattcata attttccata aaactttatt tcctccattg ttatataaat atataagtta
    10021 taattttaaa aattaaaatg cacttattgt aaatcatatc atctattata gaaaacatct
    10081 tatataacta aatataatat cttctctcaa tatgtattat acaataagtg caaaatatat
    10141 ttatataaaa tatactacat tcctataaca aaaccatcag cttcaattgg aaggtaacta
    10201 actcttctat tcaactccac ctttccaaaa aatactaccc aatatggtct aaaaatactt
    10261 tcaattttaa ctatttcaat ctctgggatt cctcccataa acctatgagt taatctcata
    10321 gccattttat ttgcctttgc ttttactctt cgttcgtcct tatcactata ctgcacaata
    10381 ttacaatcca cattttttac aacttcatat gtaggaaaat cttgtactat actaccagaa
    10441 cctgtagaac cattcactaa aatttttaat tcatttatat cattagaata tctcctattc
    10501 ttgaaaatgg atttcgtttt gtgaatttta agtgtgataa ttttaaactc tatataatga
    10561 agtctaactt catgagcata tttgttcttt aatattagct tactaaataa agaatttttt
    10621 ccacttttag ctatctctat agcttcttct tttgtaattc ttataggtaa cgctcttata
    10681 tccataaaat cattctcttt cttatttttt tcttgtcatt tttaaaactc tatatatata
    10741 atcaaaaaat attacaatta caactgccat aactccatat ggaattattt ttccaactat
    10801 aatatcattt ctagtcaata taccataaaa tataaatcct gttattaaaa agtacaataa
    10861 tactagaaaa ccgtctattc caagccattt tatttctaaa tcttttttta ccttagacac
    10921 taatttagac atagcagttt ctccttttat agattatttt gtgtatttaa attttcaccg
    10981 attctattac caatcatcca tgcaataaat gaaaaaggta tagctatcac aagaggctct
    11041 aactcaaagc tctgaactgg aaatatttta aaataaccaa aagtcactcc accaacaatc
    11101 attcctaaaa taccacccat agatgttctt cttccatttc caagaagccc acctactata
    11161 ggtacaaatg ccgaacccat ccaaatactt cctacaaata tacatgcatc atatacattt
    11221 tcaaatctaa atgctacgct aagccccata actgctgcta aacatacacc tattctagta
    11281 aactttacta attgtttctg agataactct ttttttgtaa ttcttccata tatatcattt
    11341 gctaatgtag ccccagctgc tagataatag ctatccaatg ttgatattat agaacctaaa
    11401 attcctatta taaatattac tcttattcct tgtggtaatg tatctaaaac taaagttata
    11461 tatccagcag ctggagccat ttctggataa gtagctctta ctataagtcc tgtcatagtt
    11521 aacaccatgt caaaacatat ccatagtgaa aaacaggtta ataaggctct tcttccaacc
    11581 ttaggagaat ctgctgctga aaatctttga taaaaagttg ggtctgaata agctccaaaa
    11641 gaatatacaa ttaacatcaa tgctttcata gctgacatac cttttgtagg gtgcataagc
    11701 tgtggtgcat ctttgagtgc ttctgtaaga ccttgaaacc ctcctatttg ttcaaattga
    11761 cctggcataa ccatagttaa accaaatacc atacaaaaaa acaataccat atctgtaatg
    11821 gcaactccca taagcccacc aagtactgta agtacaacga ctactcctac cattattaat
    11881 cctattaatt ctttatctat tttccatgct acgttaccta tagttcccat agcagttatt
    11941 tctccaagtt gtgcacaata tataaacatc aaaattgccc caattatagc aacatgcttg
    12001 ccataaaaac tttccagtaa atcaggaact gttatattgg ttcttatatg aatccttgga
    12061 ccaacccaca atgctaaagg cattctccca atatgtgcag ctattgacca aactgcccat
    12121 gcagatattc catatatagc cgcatactct acactaccta ctgtaccaac tcctccatac
    12181 catgatgcaa ccaaagttcc tactattaag gaccatggta aacttcgatt tcctaggtaa
    12241 taactttcca tatcttttac atttctagaa aaataataac ctattcctaa taatataaat
    12301 gcaaatataa ctataatccc attttcccaa aatgttaaca tatctaatct ccacattctt
    12361 atattttaat ttttaacttt aatatctaaa tatgaacaac tctacccatt tccataaaaa
    12421 taggtagagt tatataataa ttataagtat ttaagtaatc ttgttattaa gcaattttat
    12481 tgtttaaacc tttattttct cttttattta ttttatttaa agcataagta gcaaatatcc
    12541 ctataactgc acatactaca gataaaataa atatatatgt atatcctgtt tgtccatatt
    12601 tatctaacca attacctgca atagtgtaat aaaacgcatc tggagaaaat cctataaatg
    12661 aagcaaatcc aactactgct cctgtagttt ctaaagatat attagactct gaaacagatg
    12721 caaagtatat acctctaaat ccaaataaaa ttacacttag tattatcatg tttattacag
    12781 ctatccatat aaatcctgta ctcttaggaa taaccaagta agcagctgta cttactgtca
    12841 ttaatataaa tcctacaaac attactctta tagatgaacc aactttatct gttataacac
    12901 cagcaactgg agatgctccc atttttataa cataagttct tattattgat aaagcagaaa
    12961 ctagagtcat tggcattaca taaacttctg ataagtatgg acttaaataa gttaaacttg
    13021 agaatactaa ataagttgaa aatattataa gaccaattaa ccaagccttt ggtattgtaa
    13081 ctacttttaa caatgactta atacttaatc cttcatctga tgctccacta tcttttttct
    13141 cttccactaa gaaaaataga agtataccac atattataga tgctgcagaa tataaccata
    13201 ctacatattt gaatcctact gttatttcag caaatttacc aaagaaataa agacctataa
    13261 aagaaattaa tgtacctgat acacctgata acccttcata aaaaccaaat attcttcctt
    13321 gctctgactc acttccttgc attctaacaa ccttaacaga ggctgagaag aaggttaata
    13381 ttgtcgtaaa tccccataat acaaatatta aaagtatcat actatatgat gggaatgtag
    13441 agaaataaaa tccaagtatc cctgaagcaa ttaaagaaaa agttattaat tttctaactg
    13501 agaatctatc tgcaaaccat cctcctataa aataagatac cattcctact aatccatata
    13561 aactcaataa gttccctatt tgtgtatgac ttaatgctaa agcttgttgc attgggtcat
    13621 aaaatgatga tttcatatat ggtaaactat acatagctgt tgttccaaat gccaatatga
    13681 atattatcaa aaatctttta acattatttt tgctttccat tgattctctc tccttaaaat
    13741 tttattgttt ataatctcat tcatataatt ttattttaat ttttctattg cttgtttaaa
    13801 atcatttatt atatcatttg aagattctaa tcctacagct attcttatta gattttcccc
    13861 attcatttga actaatgtat gctcttcccc taagcttgtt gctattgttg caagctctaa
    13921 agagttcatt aatcttctac tagcttggtc tcttgtcaat ccactgatac catccttaag
    13981 tttaaatgaa actacgcctc cacctaaacc attcatttga gttttacaaa gctcatattg
    14041 aggatgtgat tttaatgctg gatactttac caattccaca ttctcttctt cctctaaaaa
    14101 ctctgctact gctaatccat tttcacaatg ctttttcatc ctcatatcta acgttttcat
    14161 accccttaaa atcatccatg cactcattgg tggtaaagat gctcctgtaa aacaacataa
    14221 acttggccat ctaactaaat ctattaattc ttttgaacct gctactgctc cccctaaagt
    14281 atctccatgt ccatttataa acttagtaag actatgtatt actaaatctg cacctaaatc
    14341 tattggtctt tgtacaattg gtgtcccaaa agtactatca actatagcta agcttttatt
    14401 ttcatgagca atttctgcta ttgtttttat atctactaag tctaatgatg gatttaaagg
    14461 actttctata taaactattt tagtatttgg tctcattgct tcttttacat tttgtggatt
    14521 tactgcatct acaaatgtaa cttcaactcc aaatttagat aacaactcag acatgaataa
    14581 tttagtgtgt gaaaatattc catctgaact aactatatgg tcaccacttt ttaccaatgc
    14641 taatatagta gatgttattg cccccatgcc tgatgtacta gatatggcac tttctgcatt
    14701 ttcaagcata gccaacttag cttctaactc tgtaagagta ggattacact ctcttgtata
    14761 cacatatccc catgtcatac acaactcttc aaaatgctct actgtctttg cagcaaatgt
    14821 tgctgtttga catattggtg tagccaatgc acctgttgtt gggtctgcac tatgtcccca
    14881 atgtattatt tttgtctcta aatcttgatt aatattgtta ctcattttga tttcctccca
    14941 ttttgactta tttatacaac gttttccctg ccaaatttca tcaatttagc ttagtcttaa
    15001 tacatttttt caagtatact tttttatttg ttataatttc taaaaatatg acacttattt
    15061 tgttttttta cagttctaat tataaactct atagtaacta tagagtcaat atgtttttga
    15121 tatttttttt acaatgttta aaatagtcaa tacaaaaata ggtatatctt agaataacta
    15181 aagtcatcct aagatatacc tatttttata tcacaatata aataattaca tataagcatt
    15241 agttttttta tcataaatag atttttcaag atttatcatt ttttcataaa tat
//
LOCUS       DAELTA010000096        15222 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.096, whole genome shotgun sequence.
ACCESSION   DAELTA010000096 DAELTA010000000
VERSION     DAELTA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.096"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            224..2290
                     /locus_tag="KPG13_002451"
     CDS             224..2290
                     /locus_tag="KPG13_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0986161.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGEIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            2307..4262
                     /locus_tag="KPG13_002452"
     CDS             2307..4262
                     /locus_tag="KPG13_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG0986162.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            4278..5468
                     /locus_tag="KPG13_002453"
     CDS             4278..5468
                     /locus_tag="KPG13_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG0986163.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            6630..7004
                     /locus_tag="KPG13_002454"
     CDS             6630..7004
                     /locus_tag="KPG13_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986164.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            7006..7863
                     /locus_tag="KPG13_002455"
     CDS             7006..7863
                     /locus_tag="KPG13_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986165.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            7866..8504
                     /locus_tag="KPG13_002456"
     CDS             7866..8504
                     /locus_tag="KPG13_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0986166.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            9515..10249
                     /locus_tag="KPG13_002457"
     CDS             9515..10249
                     /locus_tag="KPG13_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986167.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            11625..12881
                     /locus_tag="KPG13_002458"
     CDS             11625..12881
                     /locus_tag="KPG13_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0986168.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            12944..14137
                     /locus_tag="KPG13_002459"
     CDS             12944..14137
                     /locus_tag="KPG13_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG0986169.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            14338..14718
                     /locus_tag="KPG13_002460"
     CDS             14338..14718
                     /locus_tag="KPG13_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0986170.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         5614 a         1675 c         2668 g         5265 t
ORIGIN      
        1 tatagtgtaa ataagtctaa gtatgaatta aatatagatt aagtatggac taagtataga
       61 ttaagtatag attaagtatg aattaagcat gaattaagca tggattaaat atactttaaa
      121 ttacaaatat tacaaggaag ccagaaggct tcctaaaaaa ataaccttta taaaattttt
      181 tattttaaat ttaaaatttt ttattttaaa tttaaaataa aaaatgaaag aggtggtagg
      241 gagtttgaaa aaaattacta aagaaaggat agaaaatact atgaaaaagc tatttacgag
      301 tgaaacattt ttggaatatc aatttataag tgattgccaa atatcaccag atggaggata
      361 tacagcattt attgtaaaaa aagctgatat caaagagaat ggttacacaa gccaagttta
      421 tatattaaac aataaaactg gcgaattaaa acaaataacg tctataaact ctgttggagc
      481 atatgcctgg gaagatgaaa atactatctt gttccctgct ttgcgaaatg aaaaagtaaa
      541 agaagctgtc aaaaacggta agcaatgcat gagttactat gctttatctt taaatggtgg
      601 agaagcagaa gaacttttta gactccctat caaaggtggt aaattgaatc ctataggaaa
      661 aggtttgtat gcagttattg attcttatga caatgaccga cctattgtag agggattgcc
      721 agaagaagaa caacagaaaa aaataaatgc ttataacaaa agacattatg aacatttcaa
      781 agaaattcct tatgctgtca atggggaggg atatattagc agaaaaagaa aacgcctata
      841 tgtatacgat agtaatataa atgagttgaa ggcaattaca gcacctatgt ttaatgttgt
      901 aggtatgaaa ataagtgatg gaaaaatctt atatataggg caagaattta aagatgtaaa
      961 aggtttgaaa aatggggtct atgtatttga tacaaacaca aacacaaatg tctgtatatt
     1021 agaaaaagat aagtatatta taaaaggttt tgaattatat caaaatcaag tgatactaaa
     1081 cctaactgat gctttatctt atggaaatgg agaaaatgga gatttttata ctattgatat
     1141 tgatacgaaa gaaatgaaac ttctttcagg gcatcaacat cattgtattg gaaatacagt
     1201 aacatctgat gttaaaatgg gtgctggtca gacaacaaag gtagatggag aatatattta
     1261 ttacacttct acagtagata tggactgcat tattgaaaga atacatattc caacaggaaa
     1321 acaggaaaaa gtaacacaga caggttcagt tgattttata gatgtaaaag atggcaatat
     1381 tgtatgtgtg ggttgtttag gaaatggatt accagaagtt tatacagtag agaatggaac
     1441 attgtgtaag aaaacacatt taaatgacca tattttagag gaatataaaa tttctgtgcc
     1501 agaatacata gagtcaaaag gaagttctaa atgggaaata caaggatacg tatataaacc
     1561 agtagattat gaggtgggga aaaaatatcc agctgtattg gcgattcatg gaggaccaag
     1621 attgacatat ggaccatatt ttatgcatga gatacaggta tttacaagtg caggttactt
     1681 tgtattcttc tgtaacccaa gggggtctga aggtcgtggc aatgcttttg cagatattag
     1741 gaaacagttt ggagaaattg attatataga ttttatggaa tttacagata ctgtacttga
     1801 aaaatataca gacattgata aaacaaaatt ggctgttgaa ggtggaagtt atggtggatt
     1861 tatgacgaat tggataattg gtcatacaaa ccgttttgct gttgcttgtg ctcagcgttc
     1921 tattgcaaac tggagtggca tggagggtac aacagacatt ggttattatt tctgcaaagg
     1981 acaaacaggt gcttctcata tggaaaacca cgagcttcaa tggaaacagt cacctcttgc
     2041 ttatgttgat aaatgtgtta caccgacttt atttttacat ggtgaaaaag actatagatg
     2101 ctatatgcaa gaagcattcc agatgttttc agcattgaaa attcatgggt gtcctacaaa
     2161 actttgtcta tttgaaggag aaaaccatga actgtctcgt tctggtagac caaaacaaaa
     2221 gctacaaaga ttagttgaaa tgctagattg gttttcggta tatataaaaa aagataacat
     2281 aggacagtag aaaggagaat ataaaaatgg aaaaaataaa gattgaaagt tcagatattt
     2341 ttgaatatat attccctcat gatatttctt gttcacctga tggaaagcat attgcttata
     2401 ttatatcaaa tatcaacgag gaaaaagact gctatgaaca tgacttatat gtgatggaca
     2461 tcaaaacaga aaaacaaata catatgactc aaacaaaaga tgtaacatca tttagttgga
     2521 tttctaatac agaactactt tttacatcta aaagaaataa gccaaaagca ggtacaacag
     2581 atttttacac tatttctata gaaggaggag aagcaaaaaa agcattttct attccaaaag
     2641 cttgtagcgt acctgtatca cttggaaata aattgtggtt actgacaaca aaaaatccaa
     2701 cggattctaa aaaatcagaa ccagataggg cagtagaggg tgtagattat tggacattta
     2761 cagataaacc ttttataaga gatggagaaa atttttctca aagaagacgt gtaacattag
     2821 aattatatca agaaggtgaa aatataacaa aagccattac accaaaattt tgtgaagtag
     2881 caggtataga tgtttcttcg gataaaaaca gaattttata tactggtcag atttatgaag
     2941 attgtgcaac tcctttttct ggactttggg agtatcatat agacagtggt gaaaccaaag
     3001 aacttgtacc acaaggaaag taccaaataa gtttggcaaa atatattggc aaagataaag
     3061 taatgttgca agcttcaaca ttagaccgtt ctattacaca gaatcatgat atttttatac
     3121 ttgatttgag tactagtgaa ataaatatga ttgctagtcc agatggtatg tatgctacat
     3181 tactggatgt agatgcagta tatggtggtg gacgtagtaa taaagttata ggagataaat
     3241 ttattggtgc aagaatttgc agaactatga cagaatttaa tgagtttgat acaaagacag
     3301 gtaatatacg tatcatcaca aaagtagatg cttttacatc ttttgatatt tacgacaaca
     3361 ccatgtacac agtaatgttg aaagattatg agttagcaga aatatatagc attgatatga
     3421 ctacaggtac aatgaaaaaa atgactgctt tttctaaacc atatttagat actcataagg
     3481 tttcattacc tgaaaaattg acgtttgtag caaaaaataa agaagaagtt gatggttttg
     3541 ttataccacc tatagatgct aaagaaggcg aaaaatatcc tgctgtattg ttcattcatg
     3601 gtgggcctaa atgggcatat ggctatatgt ttacacactt gaagcagtgt gtaacatcaa
     3661 agggaatgta tgtgatttat tgtaaccctc atggtggtga cggttatggt gaaaaattct
     3721 tagaaatggt agaaagatgg gggtatgttg actatgagca tcttatggaa tttgtagata
     3781 cttgtattga aaaatatcct gggattgatg cagaccgctt aggggttgca gggggaagtt
     3841 acggtggtta tatgacaaac tggataattg gtcatacaga ccgtttcaaa gcagcagttt
     3901 ctcaaagagg tattagtaac ttgattacag cttctttaat tattgacttt ggggatagaa
     3961 ttatgaagca gacttgtgga gataagacgc cttggaatca tgaggaggtt ctttggaatc
     4021 attcacctat taaatatgtg aaaaatgtta agacaccaac attgttctta catagtgata
     4081 gggattatag atgttttatg ggggatactt tccaaatgtt tacagcatta aaacagcttg
     4141 gtgtagatac agaaatgtat ttgttccacg gagatacaca tggattgtct agaaatggaa
     4201 gaccatctaa tagaattgca agagcaaatg ctattgtaga ttggtttgaa agatatttat
     4261 aataaggggg ttattctatg agtgatatgt tattatggtt tagtgtcgta ttagttttat
     4321 ttgctattgg ggatttaatt gcaagcaaaa caaaagcaaa agtatcagct gtatttgtta
     4381 cactactttt atttttgatt ctatttgtca caaaagcaat accagctgat attattgaaa
     4441 aagcaggtat gaccgcagca gctagttgga gtgttccaat gattatgttt agtatgggaa
     4501 caatgttgaa tgttaaacaa tttatagacg aatggagaac agtattgaca gcatggcttg
     4561 gaattgtagc agttatagtc tgtgtctcat tatgtattcc tttgtttgga aaatcaacag
     4621 tattgacaag cataccagtt ataaatggag ctttacctgc tactactatt atgacacaag
     4681 cagcactaga aaaaggactg acacttgcag cagctacagc aacagttgta tttgcaattc
     4741 agaagtttat tggtacacca atagcttcaa gagcagcgtt acaagaggca aaccgtcttt
     4801 tagtagaata tcatgaagca aaatcaaaag gcattgattt agcaaatgtt gatactgata
     4861 agaaagaagc agagaataca ggcacaaaag taaaacaagc tttctgtgaa aaatacgata
     4921 aatattattc tacaaatgtt tgtatatttt ttatagcatt attctcttat ctagggtatg
     4981 aactttcaga gattattcat gtaaattatt ctattgtatg tcttgttgtt ggtgttattg
     5041 taacacgtat tggtattgtg ccaaaagaca ttttggaaaa aggaaaaatc aagggattta
     5101 tcaacatggt tgtgtttgca gctgttattc cttctttagc aaaagtttct ttgacagatt
     5161 taattagtct ttttgtacca attgtgggta tgtttgcagc ttctatcatt ggtatattcc
     5221 ttatgatgaa ggtgttacca ggttggaaaa ttatcggttc taaacctctt gcttttggag
     5281 tagggttttg tcagatgttg ggattcccaa ctacatattt aatttcaaat gaagtgtgta
     5341 atgctgttgg agaaacggaa gaagaaagag catatttgat gtctaagatt atgcctaagc
     5401 tggtagttgg tggtatggct tgtatgattt ctattgtggt agctggtctt atggtaccaa
     5461 tgttgtagat aggtttcaaa gttaaaaaag tggtattaat ttataggaga agctaaaaag
     5521 tagtattaat ttataaaata attttatatt agctaatttg ttataagaat attttctttt
     5581 tataattata atttgatttt ataagagagc agatttttta tttgctatgc taatagttat
     5641 aatttgtttt tatttgctaa aatgcaactt taatttgatt tatgtataat atagggtatt
     5701 taataatatt tttgaagaat ttttataaaa aattaaaaag aggagaaaaa agggagtgcc
     5761 acaaagtgaa ctttttagtt ctaaggcact cttttttgta cgaaatcgaa tatattttca
     5821 acgttttaaa agtgagaaag agacttatct ctagtttgag atagccaatt ttaattatta
     5881 aaattatttt ttgttttatt tatcaaagag tatccgtgtt ttagaagtta gtatttttta
     5941 ttttatatat ccaaggtttt ctgtttctaa tgttttcttt tttaaaataa ttagggtatt
     6001 caattatggt aaaaggtgga tggtattttg cagtgatatt atttaaagta cagtattgaa
     6061 tgttgaaatc ctcacatttt agagtattat cttttgtaat caaaaaaatc ttctatactt
     6121 atagcaaggt tacttctgca aatattccat gcatcataat atccaaattt aaattttttt
     6181 aatatagagt ttacctctag tctacactct tgaataactc tttcctctat ccaaattctt
     6241 ataaattcat cccatttatt actatctatt tattactatc tatttattac tatctaaatt
     6301 ttttagtatg tttgtaatta gaaatggatt tattccttta tgttttttat aaatagttta
     6361 taacttaatt tatcatcaaa caaatcattc atacaggatt cctctttttt ttagatattc
     6421 aattcttact tttaataaat atttaataca ttctatctta tgtggtttta aaaagtgttt
     6481 atagctatca acaatctcta atatttcatt catatttatt ttgtcatagt aatattttac
     6541 atcttttata ttgacaaaaa agtgctctaa gtgtatagtg tgtatataac aacatcacac
     6601 tgaaaatgat aaaaaggggg tgataaaaaa tgaaagtgct catttctaac acatcagata
     6661 agccattata ccaacaaatt aaagagcaaa ttaaagaagc aatatttact gaagaactga
     6721 aagaagggga tgcaatgcca tccataagaa attttgctaa tgatttaaaa gttagtattt
     6781 tgactattag acgagtgtat gaagaacttg aaaatgaagg ctttcttaca cgccaagttg
     6841 gaataggaac atttatttca actggaaatt tagaaatact aagagactca aagagacgta
     6901 tagttgaaca aaaaatggca gatatgatta aggatgcaaa agcactagga attacacaga
     6961 tagagttaca agaaatgatg gatattctat atcaggagga ttaatatgga aaatatttta
     7021 gaagtttata acttaaataa gacttatacc gatttttcat tatctaatat aagctttgca
     7081 ttaccagaag attgtataac tggatttata ggtgttaatg gagcaggaaa aacaacaaca
     7141 atccgttcga tattggatgt cgtaaagcgt gatagtggaa aaataaaaat ctttggtaaa
     7201 gatttcgatg gaaatgagaa ggaattaaaa aatcgaattg gagttgtatt tgatgattca
     7261 tatttctatg aggagttgtc aatggcagaa atgaaaagta ttattgcacc ttcatatgat
     7321 aattggtgtg aagaagatta tcaacaaaat atgaagcgtt ttaatcttaa cccaaaacaa
     7381 aagattgata cactttcaaa aggaatgaaa atgaaatttg cattagcatt agcattatcg
     7441 cataaagctg agtttttaat tatggatgaa cctacaagtg gtttagaccc actaatccgt
     7501 agtcagatac ttgacatact aacaggatat atgaaaaaat gtggtcgtgg tgttttcttt
     7561 tccactcata taacatctga tttagacaaa atagcagata tgattgttat gattgacaat
     7621 ggacgtatta tattagagcg tgaaaaagat actctattgg acgaacatag aaaagtaaaa
     7681 ggggatattc aatttctaaa tgatgaaatc aaaaaattat ttttaaattt agaaattaca
     7741 ggttatggtt ttatagggat tacctcaaaa attaatgaag tgcaaaaaaa tatgccagat
     7801 attttaatag agcgtcctac aattgaagat attatgcttg catatattga aaggagagag
     7861 taattatgtt gattcattta gttaaaaaag atattctgat tgtaaaaaaa tatgtattgt
     7921 tgatattgtt tattgcaata ggaatcccat tatttacatt gtggcgttta cctccatttg
     7981 cacaaatatt gggatttgta ctatccatta tattttcaga atttatgctg tgccagtatc
     8041 tattattaaa ggagagtcaa tattcaaaag catctgtatt actttgttca actccttatc
     8101 ctagacgtgg cttagttcaa tctaagtatg ttttatttgc aatggtattt atctattgta
     8161 cattagttta ctggattgaa aatttactta ttccacaaat aggaactttt gacttaacat
     8221 atattttgat tgttttatta ataagttcta ttatttatgg agtttatatg cctattcaat
     8281 ataaattagg atatgaaaca acaaaattct ttttcgtggt gataatcatg gcttcatcat
     8341 ttgtattacc attaactata gctcacagca attttgaaat tccatttata tatcagtatc
     8401 catccatgat agtgaatatt gtattgttcc ttttaagttt aattatactg ttagcttcaa
     8461 tgtttatttc tattaaaatt tttagtaata aggagcttgt ataaggtaag ggggactttt
     8521 atatgccaca tttaccagat tgggctgttt tactttgtat tttacttcca atagtatata
     8581 aagcttgtca gatatttaaa agtaaaaaat agagtacata tttatattgc catttttatt
     8641 gagtgaagtt agatggtatg aatttaaaag gttacttgaa aataaagtta cttggtgcga
     8701 gagagtaaca tcataatatt atagattata tgtttcttgt gaatcaattt gtatttttta
     8761 atagataata tccagtaatt ttattttcca ttgattattt ttcaattatg agtatgattg
     8821 tgtgtattgt ataccatctt ttaagatgtt tttaaaaaat tatatcgtca aaatataaat
     8881 ctaaggaatc ctataaataa agcttttcaa ttttagaaaa ctaataaaaa gtaggaagtt
     8941 aataaaaaga agctgtctca aaatagagat aagtttcttt ttcattgctg aaatgacgaa
     9001 aatataattg atttaatata aaaaagagtg ctttatgaac tatttatttt gaacagtaga
     9061 tacacttgca ctcaatttag ttcctcctaa gctttatgaa ctaaaagttt attttgaagc
     9121 ttatttttat taaaagagat tttatttttc taaataatca tctatataca gtattgcgtt
     9181 tcttttattt attcataatt tattttctta ttaaagtttt tctatattca tattttccat
     9241 attactttac ttttaatttt atacgaatta tcaaaaataa aatagttatt agaaaaaaat
     9301 aaaagtaatt gcaaagcttg caattactta cttctatatt aattactata ccataaaata
     9361 acaaaaaaat caagtcgagt aatccttcaa cttaagtgat atactaggta ttaataattt
     9421 ttaattaata aaatataact agaggaaaat ctacattttc tcaatttctt taattaaatt
     9481 gacctctaat taagtaatta taaggagaaa aattatgaat ttagcaaagg gattagatgt
     9541 tttaaaaatt tcatcaaatg tattcggtga agataaagtt atgtatattc ctgtaatcta
     9601 cacagaagat gatgcaacac ttattgacac aggtcttccg ggtcaaggtg atttaataat
     9661 tgatgctctt aataaaagta acacgagctt tgataagtta aaaaatataa taattactca
     9721 tcatgatata gaccatatag gaaacataaa ttatctaaga gaaaaatcta agaataatat
     9781 caaagtatat gcatacaaaa gtgaaatcag ctatataaca ggagaagaaa ctccttttaa
     9841 actttatatg ttggaacaaa tggtagataa aattgatgat aagatgctta gtatgctaaa
     9901 tgttatggga cttggattta aatcatcata tactaaagta gatgtgtcat tagataacca
     9961 tgaaaaattg aatctaggtg aggaaataga agttatacat acaggaggtc acactagagg
    10021 tcacatatgt ctttatttaa aagagtctaa agtacttatt gctggagatt tgttacaggt
    10081 agaaaatgga gaacttaagc ctgttgatgt aatgcacagc aacaaacaag agttaaaaga
    10141 tgctattaaa aatataagta attatgatat agaaacgata gtttttagtc atggtggatt
    10201 atatcaaaga aacataattg gaacattgaa aaacctaatt attgagtagt actatatgat
    10261 ttacaataat ttcttaaatt acttaaaatt atatctaaag atgtagaatc aaaattaaat
    10321 ataagtaata agaattttgc tttaaaatta tacttaagtg ttaagggtga tacgtagtat
    10381 tacaagtatt ttagaatata gttttttata gtaattttaa ggttctacaa tacttcagtt
    10441 gaaaaatatt taacagcata gtataatggg aaagaaaata ctttaaataa actttagtat
    10501 acaaagtgaa gaaaaatatt taaattatat ataatattgt agagtgaggt aaaatattta
    10561 agttatatat cataatacag agtgaagaaa aatatttaga taatgtacca taatacaagg
    10621 tgagtaaaaa tatttaaata atgtatcata atgcaaagcg aagaaaaata tttaaataat
    10681 gtactataat gcagagtgag taaaaatatt taaataatgt gttataatac aaagcgaata
    10741 agattattta aagcatgtat tataatataa agtgcgtaaa aatatttaaa gcatgtatca
    10801 taatacaaag taaagaagat tatttaaacc atatatcata atgcaaagtg aagaaaaatg
    10861 tttaaattat gtatcataat ataagcagag tgaagaaaaa tatttaaata atatatcata
    10921 atgtagagtg agtaaaaata tttaaatcat gtatcataat gcagagtgaa gaaaatattt
    10981 aaatcatgta tcataatata agcagagtga agaaaatatt taaatcatgt atcataatat
    11041 aagcagagtg aagaaaaata tttaaataat atatcataat gtggagtgag gaaaaatatt
    11101 taagttatgt atcataatgc aaagtaaaga aaatcattta aattatatat cataaaaata
    11161 atatgaaata ttgaacactc ataatattag aggctttact attttaattg ataggttata
    11221 ttatttataa cattaaaaat acaatttaca taaaatagac atatgaaaaa taaaaaatta
    11281 tataataatt atgggaatga ggttctccct tggatgataa tatcctaaac tgccaatgag
    11341 ctgatgactt ctatgatatc atagatgcca ccagcttttt ttgttgttta aatcaataaa
    11401 atggaggtga agtacttact aaatttttat tttttaaatt tgaagaaata tttaattaat
    11461 agaaaaaata gtagattata tgttaattat tttattaaga atgggttaag agtgtaccta
    11521 tatcctatca atttaaaaaa caattatgat agtcattaca ttgactacag ttgatgtaat
    11581 aaaactttca aaaaatctta tgatgcaaat agaaaggaat taaaatgata gaaaaattta
    11641 aaactttaat atggattaaa atgataataa gaattaaaag cgtttataaa acatatggtg
    11701 gactattttt tttaggactc ataggtatac cagttggtgc aattataggt cttatagaca
    11761 ccatatttgg aacggttctt ctaaaagtta cagatattcg agaaacatat ccaatgtatc
    11821 tgataccatt tttagcagta gtaggagttg ttattgctta ttgctatttt aaatttggag
    11881 gaaaaagtag taaaggaatg aatctaatat ttgaggtagg gcatggagaa gaagaaatta
    11941 ttcctttaag attggttcca tttataatat caggtacatg gttaacccat ttatttggtg
    12001 gtagtgcagg acgagaagga gttgctgttc agattggtgc aactttttca cattgggtag
    12061 gtaaaaggtt gcctataaaa aatgcttgta gtatttttct agtaactggt atggcagctg
    12121 gttttgcagg attatttgag acaccaatag cagcaatact atttgctatg gaggttttgg
    12181 tagctggttc actggagtat caatcgcttt ttcctgcgtt tactgcttct tttacagcaa
    12241 gttcagtatc aaaagcttta gggttagaga aattttcttt tgcactttct tctaaagttg
    12301 tatttgactt gtcaatattt tggaagctta ttgttttagg aattattttt ggaatggttg
    12361 gtggagcatt tgcatggtgt ctgaaattgt caaaaagaaa aataggtaat agattaaaaa
    12421 atcctatgat aagaattgct attattggag tatgtcttag tgtattattc cttttatttt
    12481 ataaaggaag atattcaggt ttagggacaa atttaattca aaatagtttc tatggtggag
    12541 aaatttattc atttgattgg ctgttaaagt ttatacttac aatactgact ttgtcagcag
    12601 gttttcaagg aggagaggtt acacctttgt tttccattgg agctagtctt ggtgtattgt
    12661 tagcaggatt ttttaatttg ccaattgaat tagttgcagc gcttggctat gctagtgtct
    12721 ttggtagtgc tacaaatact ttttttgcac ctgtatttat aggggcagaa gtttttggat
    12781 acagctattt accttatttc tttgtagtat gcgctatttc atatatattt aatatggata
    12841 aatctattta ttcactacaa aaaatttcaa caaaacaata aataaaacaa cataatgtat
    12901 gattagaaga gaaagaatat tagagaaagg atagggtagg aatatgttga caagtttagc
    12961 tttgattttt ttattaggaa tggcctcggg aggtattttt aagaggataa agttacctaa
    13021 cttattggga atgttactta caggaattat attaggtcca tatgttttaa atttgattga
    13081 taattcaata ctagatattt cttctgacct tagaaagatt gcattgatta ttatattaac
    13141 aagggctgga ttatctttag atataaacga tttgaagaaa gtgggaagac ctgctgtact
    13201 tatgtgcttt attccagcta cttttgaaat tatcggaatg atagttttag caccaaaatt
    13261 actaggcgtt tctatattgg aagcagcagt catgggtgca gtagtaggag cagtttctcc
    13321 cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa ggttatggta cagaaaaaag
    13381 tattccacag atgcttttag caggaacttc tatagacgat attttcgtaa ttgtgatgtt
    13441 tacagtattc acaggattgg cacaaggaaa tagtatatca gctattagtt ttttacaaat
    13501 tccagtatca attatattag gtgtaatagt tggagcagtg attggaatct gtttagcagt
    13561 attttttaag aaggttcata tgagagattc tgctaaaggg gtgttgcttt taagtatctc
    13621 atttttaatg ataagtttag agactgcttt agagggtatt gtgccatttt ctggattatt
    13681 agcagttatg agtattggga ttttccttca gataaaatat cgtgtagttg caagaagatt
    13741 gtccataaag tattcaaaac tatgggttgg cgctgaaatt ctcttgtttg ttttggtagg
    13801 ggcaacagta gatataagtt atgcatttaa agcaggaatt ggagctgtaa ttttaatatt
    13861 tggagttctt ttattccgta tggtaggagt attcttctgc cttattaaga caaatttgac
    13921 aataaaggaa aggttgttct gtatgattgg ctatattcca aaggctacag tacaggcagc
    13981 aataggaggt gtaccacttg caatggggat ggcatctgga caacttattt tgacgttagc
    14041 agttctagct atacttataa ctgcacctct tggagctttt ggaattgatg taacttataa
    14101 aaaattatta acttctgtaa aaggcgagag taagtaaaaa taaagattat tttaactggt
    14161 aagttatatt ttggaaaagg attatactat tgaatacaat ataaaaaaat catttgggag
    14221 attaagaagg tagaagttaa gaacagattt taaggtatgt tatgattatt agaattgatt
    14281 ttgtgaaatt attattgtaa tatttaatta tatagataaa gaagcggagt gataactatg
    14341 tgtttaaaaa aattatcaaa attagagtta gttattatga aatttatttg gaatttagat
    14401 ataaaaacca attcatatga gattattgat tatatgaaag aagaacataa tttaccagaa
    14461 aaagtagctt taaaaacttt atcaaaacta tcaaagaaaa gattcttata tgttcaagaa
    14521 acaggcaagt gtatgtatta tacagttgca ataaaagaaa aggcatattt agagtttata
    14581 tcaagaaatg tgcaaaatct tttgaagaat aattttataa ggaatttatt agtatcattt
    14641 cacgaagagg agctaacaga ggaaaagata aagtcgttag aaaactgggt agtaaattgg
    14701 gaagaagctt atgtataaat gttattattt ttattacaat ttagataatt ttggttttgt
    14761 aagctctgca actattttta gatggttgca gagtttattt ttatttttta ttcccttaag
    14821 caatacttaa aagtaaacgc atttatattt actaacttca attacttcta atacttaaat
    14881 tactttttaa aaatattttg atatgtcatc tctatagtaa tctagaactt gtttctattc
    14941 tttatataaa gtttttatga atgatatata taatattcgt tcatttgatt aaaaataaca
    15001 aaatattaaa taattctact ctgatagttt gttaaaaaaa taataaaaaa tattaataaa
    15061 caaaaaaaaa attatcttaa gagaggagaa tgttctaaaa tataaaaagg tttctagatt
    15121 tcataaaaga tactatttta gtcttgaaaa tatttagttt gaaaagattt taatttaatg
    15181 attgattaag ttaaaaatgt gtatgtaata aattttattt ta
//
LOCUS       DAELTA010000097        14057 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.097, whole genome shotgun sequence.
ACCESSION   DAELTA010000097 DAELTA010000000
VERSION     DAELTA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.097"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            389..1792
                     /locus_tag="KPG13_002461"
     CDS             389..1792
                     /locus_tag="KPG13_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0986171.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2269..3291)
                     /locus_tag="KPG13_002462"
     CDS             complement(2269..3291)
                     /locus_tag="KPG13_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0986172.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3326..4924)
                     /locus_tag="KPG13_002463"
     CDS             complement(3326..4924)
                     /locus_tag="KPG13_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG0986173.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4966..6231)
                     /locus_tag="KPG13_002464"
     CDS             complement(4966..6231)
                     /locus_tag="KPG13_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0986174.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6373..7800)
                     /locus_tag="KPG13_002465"
     CDS             complement(6373..7800)
                     /locus_tag="KPG13_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG0986175.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7838..8845)
                     /locus_tag="KPG13_002466"
     CDS             complement(7838..8845)
                     /locus_tag="KPG13_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0986176.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9042..10364)
                     /locus_tag="KPG13_002467"
     CDS             complement(9042..10364)
                     /locus_tag="KPG13_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986177.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10722..12362)
                     /locus_tag="KPG13_002468"
     CDS             complement(10722..12362)
                     /locus_tag="KPG13_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG0986178.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12518..13174)
                     /locus_tag="KPG13_002469"
     CDS             complement(12518..13174)
                     /locus_tag="KPG13_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986179.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13200..13901)
                     /locus_tag="KPG13_002470"
     CDS             complement(13200..13901)
                     /locus_tag="KPG13_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0986180.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4770 a         2650 c         1669 g         4968 t
ORIGIN      
        1 attatattct aatatactac ctactacttt gcgctacaac aatttctcta tttatatgat
       61 ttgtaaaata tagtacaaca ttttttatca ttttcatttc aactaaattt gtaatatttt
      121 attcaatact taataaatac ttaatcaata catgctttat aatactttac tcaatatata
      181 atcaatacat aatttataat ttgtaatact ttattcaata cataagtaga aatctctata
      241 tacataaatt cattttttca tacatcttac aaatttattt ttatttagat aacatgtata
      301 ttttttccat gaaaaataaa taataaaaaa tagtaaatat ggaaaatata aaattgatta
      361 acaataatta attacctgag gtgtgaatat gaaaatgcta aacaactttt ttaagaaaat
      421 caacaatcca gctcctgtct ttgcacagac aaaaatggaa agagataatc aaacagatga
      481 ttcactttct acaaaaccaa agactacatt tagagacgtt gctggattag acgaagtaaa
      541 agaagaacta tttgaaatag tcgattttat gaagtcacca caaaaatatc aaaaaatggg
      601 tgcaaaaata cctaaaggag tattatttta tggtcctcca ggaacaggta aaaccctact
      661 tgcatctgct gtagctggtg aaacaaactc atcatttttc aatgttactg gttcagagtt
      721 cgttgaaaaa tatgtaggtg tgggagcaaa aagagttaga actctatttg aaaaagctag
      781 aaaagaagct ccaagtataa tatttataga cgaaatagat gctgtaggag caaagagaca
      841 tcttgaaagt aacaacgaaa aagaccaaac actaaaccaa cttttagttg aaatggatgg
      901 atttaataaa gattccaatg tattaataat aggtgctacc aatagacttg acttattaga
      961 tgaagcttta cttagaccag gaagatttga cagacacatt catataggtg caccaaacta
     1021 tcacactaga tttgaaattc taaaggtaca tacagatgac aaacctatag acaaatctgt
     1081 aaatctagaa cttttagcta aaaaaactca tggtttcaat ggggctcatc tttcaaatat
     1141 agcaaatgaa gcagctatat ttgcagttag agatgacagt gagtgtatta catcagaaca
     1201 ttttgacaag gcacttgaaa gagttattgc agggctagaa tctaaaaact ctgcattggt
     1261 tgaaaaagag aaaaaaatag tagcatatca tgaagctgga catgctcttg taagtgatat
     1321 agtaggaatt tgtcctatac aaaaaatatc tatagttcct agaggacaag cattaggtta
     1381 tgttcttcaa ttacctgatg aagatagata tatttatact aaggatgagt tgattggaaa
     1441 aataaaaata ttacttgctg gaaaggcttc agaagaatta atatttaatc ataaatctac
     1501 tggtgcaaag gacgacttaa agaaagtcac tgaaatagct aaccaaatgg tttgtgaata
     1561 tggtatgagt aacttaggat ttatgactat tgatggaaat gataagactt tcttatgtga
     1621 taaggttcaa aaggaagcaa atcgtatagt tgaaatttgt tataaagaga ctttagagat
     1681 gttaaaggat aatcttgaag atttacattc tgtttctaaa ttcttatttg aaaaagaaac
     1741 tatgactcat gaagagttaa aagatttgat aggaaaagaa gctgtaaact aatatagttt
     1801 taaaaatttg aggctgtctc aaaatagaga taagcctctt tttattgtta aagttataaa
     1861 aatacatttg attttataca aaaaagtgcc tcataaacta aaaatttcat tttgagacac
     1921 ttacatattt taattacata ttttaatttt gtctatttac agaatattat atctacataa
     1981 aactaaactg ctgtaaactt aactacctcc agtaatgttt gaagtggtat aaaagctagt
     2041 agcctagttt ctacacttat aatttatatt ttacataact aaatttagta gatttttata
     2101 ctcacaattt agaacgattg aaaacttaaa aatttcttat cagatatcta caacttaatt
     2161 aggtatctat cacttgatta aatatctatc acttaattag gtatctatca cttgattaaa
     2221 tatctgccat ttaattaaat atctaccact taattaaata tctaccactt aaagtgtaga
     2281 acctctttca atcaattcag gttttaacat ttcaacgact gactctggat tttcttcatt
     2341 taataaggac ttaagtatgt taattgtttt ttcaatcaac aaatctagtg gctgtctaat
     2401 cgtcgttaaa ttataactct tccatgaagc catttgaata tcatcatatc ctatcactga
     2461 taaatcctct ggcaccctca acttgtactc aaaccttgca gcatccatta caccaatagc
     2521 tatcaagtca cttgtgcaaa aaattgctgt tggaggattt gataatgcta acattctctt
     2581 acctgcttca tatccagaat cataatcata gtttgcagat tcttctttaa ggttatgaat
     2641 cccactttgt cttaacttag agtaaaatcc aattttcttt tccatttctt cacttgtttc
     2701 tctcgtaaat tgaatatatc ctatattctt atgacctttc ttaagcagat attcagcagc
     2761 cattccccca ccttcaattg ggtctacata gactgtactt atttttatac caggtataaa
     2821 acgattaaaa agaatcacag gaatattgtt ttcttcacaa gtctcagcca tcaccttatt
     2881 catggccgaa gctgtaataa tcacagcttc tacttgaaac tgaattactt ttgaaattat
     2941 actatctata ctttgttgtc taggaacttt aaaaacgaga cactgtttac ctatctcttg
     3001 aattttttct acaaataaat tgataattct attgtaaaat ggacctagac tatcaccaac
     3061 tatcaatcct ataacattag tttttccact aatcattccc cgtgctacca aattaggaac
     3121 atatcctatt tcatttgcta cacgaaaaat tctttctctt acttcaggtt ttacgccctt
     3181 tccatttgca ctaccaaatg ctcttgaaac agatgattga gatactcctg ccaatcttgc
     3241 aacatctttt gaggtagccg atttattata atgaactttc ttttgattca tttagtattt
     3301 ctccttaaat atctagtatt tcttcttaaa attgtatcaa aaataatata atatattgga
     3361 taataaaaaa agctaataca attaattctt gtttacctaa agaaaatggc actatagata
     3421 ctccatctac tttatccatt ccttgagaaa taaggtgatt tctcatacca aatgcagtgt
     3481 attttttaat atcatctgca ttatgatttt tctttaaaat gacactaaaa atatatccaa
     3541 atatcaatgt tattgtagca cctattgctg gattccacaa ccaactcaca ttatatttat
     3601 tagaaataaa aaatcctaaa actaacccta caataaaacc aatagatact cctttatcat
     3661 tagctttata tgtaaacatt gctagtacaa atgcacctgt agctggtcca gcaaagtatg
     3721 agatatagtt accaacaagg tcaagaattg atttgatgct tcctccaaat cccataataa
     3781 taaaacctgt cataacaact cctataacta cagttataat cattgtaact tttaatgatg
     3841 attctttgtt atcttttttg aaataaaact cataaatatc cttcgtaaaa acagttgtca
     3901 tactgtttaa tatagagtcg atactagaca ttgctgctgc aaacacacct gcaataataa
     3961 ttccaatggc tcctactgga agttcattta aaataaaagt aatcattatt tcatttgaag
     4021 tttcaaatgt ccttccccca taaaaaacaa ataaaattac tcctaccaat agcataaagt
     4081 agtaaactaa attcataaca aatgcactaa ctacaaaaga attttttgca cattttaaag
     4141 atttagatgt taaaactctt tgtacttgag attggtcaaa acaaaagtat cttatccaca
     4201 ttacagttcc accaattaaa gtagcccaaa aagtatttgt cactgaaata tccctactga
     4261 aatcaaatgt atttaatttt ccactctgtt gtgcaatttg taatgtatca aagaaactta
     4321 gattcattcc ttttaaggct acaataatta ctataaatac agcacttatt actactatca
     4381 tttgaatact atcagtccat ataactgcct taatcccacc taatagagtg tatataattg
     4441 atacaagtac aactattggg actaaagcat aaagtgacca gccagtaatc atttgaataa
     4501 taagcactgg aatatatacc atagaactag cttgaatcaa tgaattaata aaaaattgaa
     4561 taatagttaa tcctcttgaa taatttccta aacgaagtcc catatattca tatatagagg
     4621 ttatttttaa tttatagata actggggtgg taatgctcag tgcaaaaaat attgctaaag
     4681 gtactgtaat attaaccatt actggataca ttccatctac atatgcccat ccaggaccac
     4741 ctataaagcc atttgcacta atcatagtag ctgcaactga taaagcaatt ggaatccatg
     4801 gcattgacct acctgccaag aaataatctt cttgattttt attatctttt cctgaataat
     4861 aaccaataac taacattaat agcaaatatc ctattataat acatacatca attgatgaaa
     4921 tcataagcca cccctttcta agtaacaaaa taaattccta gtattttatt ttttattatt
     4981 taacgtctct ggtaagaata accatacaat agcacctgct acaaagactg cgattgatat
     5041 gtaaaagata gtcatcatac ctaaagtacc agaagctgta ccagctatag caggtattgc
     5101 aaacgtagat ataatccttc ctccattaaa tgacatacca gatgacaacg aacgatattt
     5161 acttgggaac aactctgtgt agtatgctcc ccatgctcca gaatatccaa cagcaacacc
     5221 tagtaatact gatatcatat agaatgttgt tgtattatct ccacctaatt ttgtatatat
     5281 aaaaacaaat actgctgcta atgccatacc tataaaaggt atttttcttc caaatttatc
     5341 tgcaattgaa ccccagaata aatatcctac taattgacca attccttgta aactatatat
     5401 agactcagcc actgctgctg acattcctat tcctgtcact aggtatgtag ttgcattatt
     5461 tgaaaatgct gaatatgctg taaaatttgc acctgataaa agaatcgttc ccaatccaat
     5521 ccataagtat tttcccttat acatacttat atagctaatc ttttcaccag tttcttttcc
     5581 ctttttaact aattcctttg tatactccaa atcatctggc acaaagaaga ttgctaagat
     5641 agctccaact ataggtggta taatcgcagt atacatcaat atttgccaat ttgcatcacc
     5701 taaaacgcca tatactttcc caccaaaaat cattgctaat gaaaataaag cagttaatat
     5761 agcagctaac ttaccacgat atttacttgg aaacatatct gcaactaaag gcatagctgt
     5821 tccaaatacg cctcccaaca ctactcctgt acacaaacgt gctattttcc ataacataat
     5881 atttgtagga gataacattg gtataaatgt tgtcaatcca atcagtaaag caacgctaat
     5941 taaagtcctc ttcttcccaa tttttgtaga aatcacccca aaaataaatg tacctggaat
     6001 tgtaccaaaa gacataactg caaatatttc ttctgtttgt tttaatgaaa aaccaaatcc
     6061 cccatctgct aaagcataaa tcattgttgg tttaataaaa gtacccacca taaaattgta
     6121 gcaataaaaa aagtatatta aagcaattga aaaaaatgca attcctcttt ttaggtttga
     6181 atagtctttt cctaatatga tatcattttc tatttcaacc gttgttttca tatttttata
     6241 attccccctt ttaaatttag tcataatttt gctaaaagtt taaacatcta tttttcatat
     6301 acattcccca cttaaatttt ataaagcaat acttccctat atatttcaaa actatttata
     6361 ctctaaacag ctttattctt ctactcccat ataaatttgt tttaatacat ccataccatc
     6421 aaaagttatc cactcctcct ttactttacc ttgtagtata tgataatgat tgattcccat
     6481 tacttctata tgctttccag aaggctgtcc aaaaaatcct ataccttcat ttattccacg
     6541 taatctccat cttacagata catcataacc ttcatttttt tctctttgat tacaagttac
     6601 tctttccact tcataacttc catttggaat tgatgcaaat aaacttacaa tcattccttg
     6661 aatctcatca tagccattta aatctttatc acatataaaa tgtactactg cattatcatg
     6721 atagtatttt ttaacttcat ttatatattt ataattataa atacggcttg tcatttcaag
     6781 catcatttcg ccaacagaat catcttttgc ttgatactta gttggcataa attgaccatc
     6841 catagattca cagataccat aagttgattg taaactaagc actttagatt cacttgcctt
     6901 tgccatactc tttgctactt cctgagggtt taatcctaat tgagtaacaa tccataaatt
     6961 atctctaacc aaccattctt catggattct attgttagtt gtggcacaat caattacagt
     7021 tctaaagttt atttttctac ctgttgctgg tccaaaatta ctatcaccta agttagttgc
     7081 tgtagacaaa acacggtgag aagataaaaa accatcagca ccaaagttag accatattac
     7141 attttgacca attaatcttc tatcaggaaa cgcatgtaat gtttgaagag tattggaaat
     7201 aacatcttta ataccaacta aatttgaaga gccacaatgc attgtaacat tattgtgata
     7261 tgtatcataa ataacaccta tacctttttc ttcccagata ttatgtgtaa tcttcataat
     7321 ataatctaca aaatctctat acttagaatc aaatccaggt atttcctgtt ccctttttgt
     7381 ctgttttgaa aaatcattgt aatccattac tccaacaggc gttacttcta atgaatctcc
     7441 atagtaaatt tttgtagatg acatattatc ttccccctta aatataactt ttttattttt
     7501 ttcagtaaga gtatttgtat ttaatttatc tgtatccact gtatctatgt taactttatt
     7561 tatgtcggat ttatttgtgt ttgttttatc tatattccct ttatctatat ttcctttatc
     7621 tatatttctt ttatatgtgt tcgttttatt ctcatttgtt tgattttcat ttatattact
     7681 aaaattagtt tgattcattt tagctttgtt ttctttaacc gtacatgatt caagcccact
     7741 tgtagaaact tctttttctt cctctttact tgttgcatta tttttgttgg tagatgccac
     7801 tttatatccc cctttgtttt agaatacgta tgcataacta agcattaaaa atacttttag
     7861 ttctctcaag aatatcaagc ccttgttgtt tcaaccaata aggaatatca attaacaccc
     7921 agttttcttg aagtttatcg cccttacgat aataaacatc aacaacttgc atatctgctt
     7981 taacctctcc acctggtaat cctaaaaaac ctccacatgg tacattagtc agattaggcc
     8041 atccaaagaa acaagcaaaa ttaccttctg caaatctaca tacatgccca ttaaaagtct
     8101 ttccttttaa attatttcta aatggtagct ggtgttgctc ttgatatcta ggtatagtat
     8161 aagaagcacc aattccacct ggaccatacc aaatcatatc tttagcccaa gatttttcaa
     8221 gtatttctgg tgggcatccc atagcaccac tttcatttag ttcagataaa tcatctacca
     8281 ttttatttac taatgctaaa gtttttactc cttcaacttc atctgcatct tcaaaaagta
     8341 atccattatg gtcaattggc cctggatata caaaatatga acctgtttgt ggtggcaatg
     8401 gattaactcc tgcttgaatc ataaacccaa ttatatctac aaataatcca gttttagcaa
     8461 ttttaccatc ttgaacacag ttaaattctg catatcttaa actaatcatt ttacgagtat
     8521 gtttaatgcc aagccaatcg ttatcaaaaa gtcccataaa atgccccata ctcattaccc
     8581 attcactatt gtcaatctca tttgttcctg caatgaaaat atcttgtcgt ctttgcatat
     8641 tactcaatga tttttttaaa ggcttccaaa atacatctgc tacattttca gtgccttctt
     8701 gttcacgaaa tggatacaca cctttccagt tataatctga tttcatatgt tgctttaaga
     8761 cattttcaac ctcatcccca gtagcatttt ctaatgcttc aaaataattt cttacaactt
     8821 tttttgaatc ctgatatttt cccataatta tctcctccca gatttattta agttataaat
     8881 taactttctc atttcatgca tacgatttcc tttttaatga ttttttagct gttttaagtt
     8941 tattttatga atacatatgc atttttctaa atattaacat atatctaaaa aattgtaaag
     9001 aaaatgttat cataaaaatt taatttttga cttatatcta tttaaaacaa cagaaaaggt
     9061 ttaaaacgta atattctact gggtctacca tacttgctca taagctgttc actcactacc
     9121 tcacaataac cagaatcaca caatctaagt ataatcctgt ccatagttct aatacttaca
     9181 ccacatatag tagctaattc ctttgatgta aattcagttt ttccactatt tattatttta
     9241 ttgaatactt tatatacagt attagcactt agccctgtct ctgtagcaat ttgataaaaa
     9301 cgttcatcaa aactattttt ctctttgaca gtaggatttg gctttagtgg tcccataatt
     9361 tctccatttt caaaaactac atatgctgca tcattttttt gactttgagc catcttaata
     9421 ctctcataag cgttaaattt agcttcattt gctgtttttc cataaccaat tcctatataa
     9481 gtgtttataa ctgaaacttc acgaatcata tccaacaaat aaatatctct gtatttcttt
     9541 gtttctaact caagtatttt tttagttgta aaaatcataa attcacttct actctgctct
     9601 accaaaacac catctatacg gctattgaaa tggtatagtt tatcaagaat cttaatcctt
     9661 tgtgaaagat aagcgtactc atcttccttc attagtgaat attctgaagg catgttcagt
     9721 ttaatagcaa gaacaataat ctgattctca ttgtttttct ttgcaatata acgcatctga
     9781 atgtttttta tactttgcac cattacgtcc tcagaaggca aggctaaaac tactggaata
     9841 ttatcttttt tcattatatg ataggcctct tcaagacctg taatacaaca acatacatca
     9901 ttattttgat aattatattt atgaaattca agcacaaact ttatatagtc ctcttctaaa
     9961 tacttttgct ctgcaaaaaa catatgttca tccttaaatt caactccaat ttctttatac
    10021 aatctcttca tcatagcctt atcataagta tctatactaa gtctactaat atcgaattta
    10081 agcaaatatg taatttcaag caacactctg tacaatgttg tttcatgtct aggaatataa
    10141 tcagacaata caaaatcttt ttgtcttttt tcaaaaaatt taaatggcga cttacctgta
    10201 aaaagaactg catccactct acctctatat ttttccaata aatcaggtac ttcaatatat
    10261 ctatcatatc taagaaaatc tgcttcaata ttaggaaaat tctttttact tactgctagt
    10321 attttatcga ttaccatacc tggtcctaca taaagtattt tcatttcgtc tctcctttcc
    10381 taatgatact attatctctt aaaacatttt ctaaacctac catgactaaa gccacaggac
    10441 tcctgattca tagaattttg aataatcaat tatacctaat ataaaaagtt actttaagtt
    10501 ttttaattac gactatctgt tgaaacattt ttgtatttaa atttttgtat ttaaatctta
    10561 ttcattaata ttaattacat tttacaatta atatattgtt ttttctactt aaagttaaat
    10621 tctttagaaa acacaagtag agtaatcttt attaatttaa caaaaatagc cttccttaat
    10681 attcaaagaa agactatttt tataaaagtt cttaatgtat tctattttgc tatgctaaga
    10741 ggatttttaa ttacacgtcc agcacataca tcagtttgct ttcctcctgc caatgcaatt
    10801 ttaccattca ccaacacata ttccactccc actgtatact gtttaggatt actataagtc
    10861 gctttatctt ctattgtatc tgggtcaaat accacaatgt cagcaaaata attttcttta
    10921 agtagacccc tttctttata aatgcctaag cgttttgctg aaagataagt tatcttttta
    10981 actgcttcct caaaagtcaa catacctttt tctctaacat agtaagagaa aattcgtcca
    11041 aaagtaccaa atgcacgagg atgaggcttt ccaagagaaa gtaaaccttc tgtagaaagg
    11101 tccatactat cagaacctat acatgacttt ggatggcaaa caaccttatc tgtatcctct
    11161 tgaccttgtg tatgaacaac tacttgaatt ttactttttt cttcaatcaa aagcctaaag
    11221 aacgtatcaa atggattttc accattgatt ttggctacct ccattaaagt tttaccctct
    11281 acatatttat ttgcttcagt cattacagag gctagtacaa caccgttcca cccacctgct
    11341 aattggtaga aacattgcca tccaggaatc ccttcctcca actcttttat aattctgtct
    11401 ctattttctt gtacttctaa tcgttttacc atattttcta caccgccttc aaaagcccaa
    11461 ggaggtaaaa gtgcacccaa agatgtcatt ccagctgtat atgggtaaca atcaaaagtt
    11521 acttccaaac catctgcctc agcttcatct agctttttaa atacctcatc aatagttcca
    11581 aaattcttat gacccataac ttttaaatga gaaatatgta gaggaactcc tgcagtctta
    11641 gctatataaa ttgcttcatc aagagcttcc atttcttcat caccttcatc tctaatatga
    11701 gatatatagt atccattgta tggtatcaat tccttacata attcagccat ttcttctttc
    11761 tttgtgtatg ctcctggtaa ataaactaat cctgatgaaa gagctacaca tccagcttcc
    11821 atagcttttc taacttccaa cttcatattt ttaagttgct ctaaagtagc tggttccttt
    11881 gaaaatccca tttcattgat acgtacaggt ccttgtgcta cataagctac aacattgact
    11941 gctggagaat tttcagttaa gtaatcaaag tattcttgca ttgtattcca atgaagctct
    12001 aattttgttg gaattgtacc aattatccta gttccaagat aatcaattaa ctcttgcttc
    12061 ttctcttctc ttatcggagc aactcctata ccacagtttg caataacatc tgttgttaca
    12121 cctgtatgga tacggcttct tgtgtattta tcatacacaa gagataaatc agaatgagta
    12181 tggaaatcta taaaaccagg tgatacaaat tttccagttg cgtctatagt ctctttagcc
    12241 tctttttgac ttaaatctcc aatagccatg attttgtctc ctacaatccc tacatcactt
    12301 ttatatcttg aatttcctgt accatcaacg attattccat tgacaatttt aacatcaaac
    12361 atgctttttc tcctttcaag acttatttat attcatattt cttaataaac aatcctattc
    12421 ttttttctca acaaataatt ctagtctttc tccttaataa acaatcctat tctttttctc
    12481 aacaaataat tctagtcttt ctccttaata agcaatccta ttcttttgtt ttattgttat
    12541 caattacaaa gaagaccctt gtgataagta tcccacctaa cacaataaac aatgttaata
    12601 atccacctgg tataccaatt ttttcaccaa agtacataat tgctaacgct acagcaatcg
    12661 tacatccacc agctcgaata tccttacgtg caatatcaac ataaattgct ccaaataatg
    12721 ctggaagtat ataattaaat gaattagtaa caaattcagg taacaaagga attactttat
    12781 agcctacaat ggtaaaaatg gtaatcatag aagctgatac aaagattgaa caacatgtac
    12841 caattgttcc aacaacttca ccctcatgag ttcctgcttc tacacctgca attttctgtg
    12901 ccattgatgc tgcaggcata cgtatatctc caacagaacc tgcaagccaa cctatataag
    12961 aacctggagc tcctagtgta ggataataag ctataggttg aacaatccaa ctaaccccat
    13021 aagtcgcagc gactaacccc caaatttgaa atataacaga aagcattggc atctgaccaa
    13081 agcgaatacc tacatagata gcgggaataa agtttgcaat aattgcaaga cctatagaaa
    13141 ttctccctat ttttatcatc tttttatcat acataattat cacctcttat ccgttttaat
    13201 cacgataata taatctgtgt accaaacata ccaattaaca tggcaatacc taaggataat
    13261 tcttgtaatc gttgattctt tttaaataaa gttgtcaata ctagcataac tatagctgaa
    13321 ataattgctg gtgtaagttt tggtatttca aatgttttta tttgatttga aagaaggtaa
    13381 gcaaataatc caactgtagc acctgcaagt agttttttaa cccaagtagg attatacttc
    13441 atgttcatct tttcaacaat tccacgcatt ctatgaccca aaataaatac aacaaatagc
    13501 caacctaagt tatttaaagc catcccccat agtgcatacg aaaaagcttc tgctccaaat
    13561 gcagcactac ctacttctac cccagataaa ttggatgtaa gagatattac tccaagctct
    13621 gttctagctg ctccgacatc acagagcctc atccaagtcg taggagcgcc aatcacagct
    13681 accagtgata ataatgtaat aacaggagat agagaaggtc caatggctgt aacactagct
    13741 gagcgaattg ctgctttaat tcctttcttt tcaataccaa gatttgaagc ctcttttagt
    13801 gcctctttca taaatacgat tgcttgaaca actgaaataa ttactaaaaa actagaaaca
    13861 atccaaagac ctgggctatt aatcatttct tgaaacttca tactttattc ctccttaaaa
    13921 taaatttttc agcattttat tgtgagcttt ctttctgtaa tcttgttatg tttttatatt
    13981 gttttaattt attttatcta agatatagta tctttaatag gagattatct gtcttaaagt
    14041 ctcactaaaa acattaa
//
LOCUS       DAELTA010000098        14039 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.098, whole genome shotgun sequence.
ACCESSION   DAELTA010000098 DAELTA010000000
VERSION     DAELTA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.098"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(188..919)
                     /locus_tag="KPG13_002471"
     CDS             complement(188..919)
                     /locus_tag="KPG13_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986181.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            complement(1196..2503)
                     /gene="grdB"
                     /locus_tag="KPG13_002472"
     CDS             complement(1196..2503)
                     /gene="grdB"
                     /locus_tag="KPG13_002472"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1454..1456),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG0986182.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(2589..3875)
                     /locus_tag="KPG13_002473"
     CDS             complement(2589..3875)
                     /locus_tag="KPG13_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG0986183.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(4154..5476)
                     /locus_tag="KPG13_002474"
     CDS             complement(4154..5476)
                     /locus_tag="KPG13_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG0986184.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(5476..7470)
                     /locus_tag="KPG13_002475"
     CDS             complement(5476..7470)
                     /locus_tag="KPG13_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG0986185.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(7557..8804)
                     /locus_tag="KPG13_002476"
     CDS             complement(7557..8804)
                     /locus_tag="KPG13_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0986186.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            9032..9577
                     /locus_tag="KPG13_002477"
     CDS             9032..9577
                     /locus_tag="KPG13_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986187.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            9591..10277
                     /locus_tag="KPG13_002478"
     CDS             9591..10277
                     /locus_tag="KPG13_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG0986188.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10401..11021)
                     /locus_tag="KPG13_002479"
     CDS             complement(10401..11021)
                     /locus_tag="KPG13_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986189.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(11011..12033)
                     /locus_tag="KPG13_002480"
     CDS             complement(11011..12033)
                     /locus_tag="KPG13_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBG0986190.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            complement(12034..12672)
                     /locus_tag="KPG13_002481"
     CDS             complement(12034..12672)
                     /locus_tag="KPG13_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG0986191.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(12790..13941)
                     /locus_tag="KPG13_002482"
     CDS             complement(12790..13941)
                     /locus_tag="KPG13_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0986192.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         4579 a         2436 c         1398 g         5626 t
ORIGIN      
        1 tgtatttata atgattaaaa aaattgaaat tatcgaatat aaaataaaac accaaaattt
       61 tgaattaaaa tttgtaaaat tgattagaaa atagtattgc actattacca ttaaaattct
      121 atatacaata aaagagaaga aaaaactgta ctttaaaaat aaatcggaag aaaataaatt
      181 aatcatctta tatcctccta tttttcatga actcaagcat aacttttgaa aatatttgtt
      241 gacgtagtct agaaacaggt attctatctc cactttccat aactgcatta ccattttcat
      301 atccatatac atattgtaaa ttgattatat aactacgatg acacctaaaa aaatttgttg
      361 gtaaacttct ctctaaattt tcaatagtct cataataatc tacagtctct ttctttaaat
      421 gaacataaat ctttcttttt ataacttcac aatataaaat atcagcaagt tttaatttcc
      481 aatatgaata acctttttgt attgtgatat aattactttc attatcatca agttttgtaa
      541 ctattttatc caaagtttta tatagtttct cattagatag aggttttatc agataattta
      601 tagcatctac ttcaaaagca tcaaatacaa attcatttaa aactgtaacg aaaattataa
      661 agcactcatt accatatgac cttaattttc tagctgcctg cattccgtta atctcttcca
      721 tctgtatatc taaaaataca atattaaact tttcatgatt gcatagtaaa tcttctactt
      781 tattaaaaag ctctatatta tataataaat tcttctgctt catgtataat tctatatttt
      841 cctttaaatg atttagcata taggattcat catcacaaat ggcaattgaa atcaatatat
      901 acaccttctt tcttatcatt attctaatta ttatataaag ttaatttttt ctgttcaatt
      961 aatatgatct aatatgttga atatttgttc caaaatgtaa taatctcgct tttcatagta
     1021 tatctaattt gaatttgtgt atcaatatta taataaacta atttatcact tttataaatt
     1081 taatttaaaa aaatgcaaat ttcgaattta cctaataaaa aaagatatcg taaaataatt
     1141 ttaaaaaatt attttttgat atcttttttt atttcagtgt tgtgttgtat ttttattatt
     1201 caaatactgt ttgttcttct atatcttctg ttagtgcatt taaagctttt aaaactatat
     1261 tctttctaat ttttttactt tcctcttctc ctaacatagg atttccaagt ggatatggta
     1321 tccctactgc tggaacaatt ctattagctc ctattgtaag tgatattggt actactgtac
     1381 atatatgaac aactgctata ccagctctct caatctcttt taccatcgtt gctccacaac
     1441 gagtacaagt acctcatgta gaagttaaaa ttacagcatc tacatcatct tctaacaatt
     1501 tcttagaaaa ctcttctcca aatctcttag aattgccaac actcgttcct gttcctgtag
     1561 tagttataaa ataatctact aattctccaa ttacaccttc tttttcaagc tctctaacta
     1621 catctagtgg tacaactaaa tttggatttt caaggacata tgctctgtca taccccccat
     1681 gtatagttgt aaattcttta tttgacatag aatccatatc tgttaaatcg taaattccat
     1741 atttagtagc acttgaagat tcaattttgt ctggattatc aattggaact atccctccag
     1801 aagttactag ggcaagtttt atcttagata aatctttaac aggtttcata ggtgtaactc
     1861 tatcaaattc aggcattgga tactcagttt caaagctttc ccctttcatc tttttaacca
     1921 acatatcaat tgctctttca gaccctctaa gttcatggaa gaaatttttt cttattcctc
     1981 tcattatata tccttcctct tcaggaggtc ctatttcctc acctctaacc aatttaagac
     2041 ctagtttcgt cattattgga actacctttc taagacttgc tgctgaattt cctgtagaca
     2101 ctatatgcaa atctaattta aacatatcta cacctggatt ttctttatac atgcctgtaa
     2161 ttacaggaat atttaactct tcttcaactg ccttacaaat agtcccacaa gctactccat
     2221 atctacctgc attaaatgct gggcctgcaa taaataaatc agggctttct tcttttatca
     2281 tttttaaaag ttcttgagtt gcactctcaa tattttcacc aaaatacgta tctccgcaaa
     2341 ttacagttga gattatctct gcatcatcat taaattgact ctgaaatgct attccaggtc
     2401 ctactacacc agcccttttt tctggcggaa tatctgcttt ttcctctcca cctataccag
     2461 caaagaaatt gtttatataa tgaaccactc tgattttact catctcttaa tcctccaaat
     2521 cactttatag ttattacatc agttagacca atgattacat caacagaact ttatattcaa
     2581 atacttattt aataatatct tgctgtcaaa tgattatagc catttgctat agttgatgct
     2641 attataattt gtagctctgc atccatgctt ccgtcttcat ttaagcatcc tttataccca
     2701 cctatcatca tttcaacata atctaaagtt cctataattt tttccattgc tgggaaatga
     2761 accactagat ttccttgacc acaagaaaca actgcatctg cttccttagt agcgtctgct
     2821 atagattgac tcttgccatc ttttccagga aattcatccg ttatcaaaac tacatttgct
     2881 ccaacttcac tacattttct acaattcatc attaggtcag tatctggatt cccataacct
     2941 tcttctgtca ctagaactcc atcaactcct aaaaattcta ttaatttagc taccatatca
     3001 gagcttctct ctttatcgac taagaatacg ttctcattag tcaaaataac tcctataaag
     3061 tttaaatctt ttccatgtct tttatataag tcatgtataa ctggattatt taaatgatgg
     3121 aatgttgtaa ccttatcaca tggtgctaca cagtttccag atattatagc tccatccatt
     3181 atttcagttg gatacataaa tgtaggcact atctgtttag catccacccc atagtaataa
     3241 gtgtcatgta aaagtccttg tgattgtaac atgtgaacat atgctactgt tggcaggtct
     3301 ggatattctt ttacttgttt taataatggc tttgtctcat atacaatgac ttcatctggc
     3361 tcaacttctc ttcctgcctc acctacatat gcacctattt ttaatcctgc aattctagct
     3421 gcttcttcat aatcatgagt atctatacca tcctttgctt ttataacaat acatatatta
     3481 tttgtttttg aaaaaggtgt atatttagca gtaggaccac tcatatcaat tacgccttct
     3541 tgaaatccaa ctatatttcc acaagttaca acacaagctc ctaaaagtac atgggttctt
     3601 ccacttccta cagtcttcac tttatttata attccaggaa atattttgct ttcatctcca
     3661 attttaactc taggctctat tacatcttta actggagcta ttcttattga ttcgccaggt
     3721 cttgcaagtt caacatttac acttacaatc ctttcatctt ctaatactat cttttctatt
     3781 tcttcgctac taatatatag tactccatct tttacttctg tcttttctcc aaattcaaca
     3841 ttttttataa atatattccc tagctctagc ttcatatgaa aatccccctt aatatattta
     3901 atcgcaatta aaagtattta ttacgtttat taatatataa agcattttct atgccaatta
     3961 tttaaaatat atattctatg tattaatgtc atagaaattt tcatttttaa tttacaccaa
     4021 tattgaattt agcaataatt attgtaaata ttaattttta aaattataat aaaaaaagat
     4081 aaacagattt tttctgttta tcttttaata cagtttgaga aatttttttc tcacttaatg
     4141 agaagttttt ttctcactta atattatact ctgatatttt attggttaac tgtcttctac
     4201 taatctccaa ttttttagct gtatttgaaa tattatttcc acacaaagat aaaacttttt
     4261 caatatactc acattcaaac tcttttctta tttctcttaa aggtctaata cttattttat
     4321 catctatgta tacattattt gatattaaat ttaaattgtc ttttgataaa ttaccttctt
     4381 cacttaaaac tacaagtcta tttattatat tttttaattc tcttatattt cctggataat
     4441 tgtaatttaa taaaaaatcc tttacttcct tatcaatcga gtgtatcttt ttatcatgtt
     4501 caatttggta cttatttaag aaaaattcta taagagtagt taagtcttct cttctctttc
     4561 ttagtggtgg tatagttatt gttattgtac ttattctata aaaaaaatct tctcttatat
     4621 ttccatttga tatttccact tttggttctt tattcatagc acaaattaac ctaaagtcaa
     4681 ctttaataga tttattactt cctatcctct ctatagattt gtcctcaagt gtcctcaaaa
     4741 gttttacctg aacatttaga ggtatatctc ctatttcatc caaaaataga gttcctttat
     4801 tcgaaagttc aaatctacct tttctactat ctactgctcc tgtaaaagaa cctttttcat
     4861 gaccaaacag ctcagactcc aatagatttt ctgaaaaaga acaacaattt acaggtacaa
     4921 atatctcatt ctttcttgga cttatgctat gtatatatct agctaatata tcttttccta
     4981 caccagattc ccccaagatt aatatattaa catctttaca agcagctttt tttgctattt
     5041 ttatgatatt attaaaatct ctatttttag attccaaagt aaattcaaaa ttattttttt
     5101 gtacagaaac agatatttta cttgccttta ccttttcaac ttcttctaat aaattttcaa
     5161 tgggattact ttttataaaa taagataaag ctcctctttt catagcatca actgcatttt
     5221 caatacttcc atatcctgta actaatataa cctctatatc tctattaata gatttaattc
     5281 tatctaatag ctgtactcca tccatatttt tcatcataac atcagataaa accaaatcat
     5341 aatttttatc ttttttaatt atatttatag cttcttcacc acttagtgct atatctacaa
     5401 gatagccctt cttttgtaga atttttttca ttaccactcc atattctagc tcatcatcaa
     5461 ctaatagtat tttcattata tagtcacctc tttatttaat ggaattttta tatgaaaaca
     5521 agttccaacc cccaatttac tttcaacact tatatctcca ttattctttt taatctcatt
     5581 ataagtaata tataaaccta aaccagttcc ctcaccaatt ggttttgttg tatagaaggg
     5641 attaaatatg tcctttagga catcttcttt tattccttca ccattatctt taaaatctat
     5701 aaataaacaa tgattttttt cataacagta tataataatt tttccatctt gatgtattgc
     5761 atctattgaa ttagaaatta aatttataaa tacatgtttt aacgattctc catttatata
     5821 gcatattaaa ttatgttcac agtcaatttt tatttcaaca ttctttaact gtaacatttt
     5881 atattgaagt ttcactatat tttcaataaa attttttata tttatatgtt ctaaattatc
     5941 atctgatatt cttgcgaaat ttaacaagtt ggttattata ttacttgccc tatctacatt
     6001 actttctatt gatttaacac aatcatttac ttcttccatt gtaacgttat cttttaagag
     6061 atagcaatta tttcttatta tacccaaagg atttcttatc tcatgtgcta ccccagaagc
     6121 taattgccct atagatatca ttttgttttc tctaagtaat ttcgcctcta ctattttatc
     6181 attagttata tctttaataa gaactactat atactcagta ttattttttt tatactctac
     6241 aggaaaagtg tttatcttta aaattttatt gtgtatttct atttcactag ttttattagt
     6301 attttcagag aaggtatttt caataacacc ttttatcatg ctaatcaaat ctgctatctt
     6361 ataactcatt tcacccataa agtcatatag tactttgttc gattctacta tattgttatt
     6421 ttcatctaca agcattatta tatctgtaat actattaaaa gttgcctcta aagttttttt
     6481 attttctatt acctgttctg ttttcctttt gatttctatc tttaacaaat atgtatagct
     6541 ataaaaaaca taaattgata ttaaaattat acctataaaa agataaatac taggcacaat
     6601 tcctcgctca tacaatatat catcaacttc attatacgta gaataccatt ttttctttaa
     6661 atctttatac actccatttt tctgaagttt aaaaatgcct ttatttaaaa tactaaccag
     6721 ctcttcatat tgtttaggta cagcaagaac tgctttttta gtatatatag gattacttaa
     6781 aacactgtat ttatttgaaa gtccataatt atttatatag tatcgcaata ctggttcatc
     6841 accaacaact gcgtctactt ttccagacat taaatggttg actccagtct ttatatctgg
     6901 agtcaataaa atgtttatat cttgaatttt ttgctttaaa aaatttatag aataatcacc
     6961 ttctggaatt gctactgttt tcccctttaa atccatgtaa ctatttatat cttgggactt
     7021 tttatctttc aatatatttg cactcaaagt gtatattggg tcagtaaata taaatttatt
     7081 tgccctttct tttgatggaa tcaagtcaaa aaactttata ctatcatcta ttgaatttac
     7141 gaaagattct ttccaccaat tatttggttt aaaataaaaa tctctaccaa tttgaattga
     7201 cagagaattt attaaatcaa caataattcc cttatactgt ccatcttcct tacctttata
     7261 tctcaaagga ggtgaacttt ggtcagagct gtaaatcaaa ttcttgtgtt tttctaacca
     7321 ctttttttct tcttctgtaa atggaagtga cttcttaata tattcaaata catttagatt
     7381 atactccata tttacataca tatttgtaag tgaaaatact actaaaaatg aataaataat
     7441 ccctataata acaatctttt ttttattcac tttaaaactc caatcttctt ttttactata
     7501 ataagttcaa tatttaataa caaaatccta cttttttcat taaatattta tatttattat
     7561 aaaactaatt gaatagatgt tgaaattata gtcattacta ttcctatcaa tgcttcatat
     7621 ggaattaact taaatctttc acctatatcc atgtttatac tacctgcact agtatgaaat
     7681 agtgagccat gtggtaattg ctcgaataca ctagacccag catttactat tgatgcacct
     7741 gaaatcggtg atagcccacc atttattata gcatcatgaa aagtagatga tgctatagta
     7801 gcaccagctg ttgatgatgc tgttgctaat gacataagta tacccgaaac tggtgccaat
     7861 aaaaattgtg gcatatttaa aaattgtaaa gcacctattg tactctgttg taattcagac
     7921 atctgtataa ttcctgcgat agtccctgtt ccaagtaata atatgcatac cccttgcatc
     7981 ttagataatc caaaagttaa atattctctt gaattgttca agtttcctgt gacaattaga
     8041 gttacgattc ctcctattgg aagggctatc attgggtcta taactatagg agatacattt
     8101 cctaaaaaca atagaaatat agctataatt ggaccacata atgatttaaa taaacttggt
     8161 aaatcttctc tttgctctaa aatttcatat gattgtactt tatttccttt atttattaat
     8221 atacttgcta aaataactgt aataacaact ccaataattg ctggaattat atttgccatc
     8281 ataacactag agagattgac agaaaaatta tctgctactg ctatagtatt tggatttggt
     8341 gatattatat tacctgcttt accccctccc aacatagcta atagtattga caatttagaa
     8401 taatttaatt ttctacctat atataatcct ataggtgcta cagttattat agatacatcc
     8461 aaatttacac ctactccagc aagtaccata gttgaaatgg caatcgaaaa taaagccctc
     8521 ttttcaccaa atatttttac aatctgttct gatattttat ctactgcccc tgtttttatt
     8581 aaacttccag ctaatacacc tgatgccaaa gctctaagaa ttgatggtga tatattcata
     8641 gctccatttg ccataaaatc tacagtttgt gttatatttg ctcctccaac taatcctcca
     8701 ataaatgctc ctaacattaa actatataca gcttgaaatt tttttataat taaaataatt
     8761 gctattaata aaccaaatat agctccaaaa gtcgttattt gcataatatc ccctcctatc
     8821 ataaattaac tatcttttgc ttaattttat ttaaatatat tttaagtatt ctgtatccac
     8881 tttgtcaatt atattttaat ttatttaatt atattgaaca tagttttttc aaaacctaat
     8941 tctatatgtt ataataattt catactacat aatcatttta cttgattatt ttaagttaat
     9001 attttttaaa ttaaaaagag aggatattta tatgaatgat ttaaatttag gggaaaaaat
     9061 agctgaagtt cgaaaaaaac aaaacttaag tattcgagat ttagcaaaac tagctgatgt
     9121 tacaccatca ttattgagcc aaatagaacg aggtcttgct aatccatctg taaattcatt
     9181 aaaatctata gcctcttctc taaatgttcc tttatttaca ttttttgtat ctgaagtaga
     9241 taaaaaaaac ttgatagtaa gacatgataa tagaaaaaaa gttattttac ctggaagcaa
     9301 agaagttata tatgagattt taattccaga ctctagtggt aatttagaat ttgcaattat
     9361 ggatttagct ccaaacactt cttcttgtgt tgataggatt actcataatg gagatgaaat
     9421 tgcatatgtt ttggagggag aagttaaact ttttatggat gatgatgaat ttactttgtc
     9481 caaaggagat agtgtaaaag ttcctttagg aacaaaacat aaatggcaaa atgactcaaa
     9541 tagtgaaagt aaagtaattt ttgcagtcat cttgtaaagg agtaaaaacc atgaagacat
     9601 ataaatttat attttttgat gcagatgata ctttatttga ttttaagaaa agtgaatcac
     9661 atgcttttaa aaaacttcta tctgagtttg atttagaatt taattttgag aattatatag
     9721 agtcatatag aaatataagt gataagcttt ggctcgacct tgaaaaaaat attataactt
     9781 tgaatgaact taaattactt agatttgaac tttttgcaaa taaaatatct ttggatgttg
     9841 attctgaaac tcttagcaaa atgtacttaa attttttagg tgaatgtaca tttttaattc
     9901 caggtgcaat cgatatatta caatatctta aaaaaaagta tactattgtt ataataacaa
     9961 atggaatttc tgcagttcaa aataaaagac ttgaaaattc aaaaataaaa ggatatattg
    10021 atggaatggt tgtatctgaa gagttgaaaa tatcaaaacc aaatcctgaa atatttaaat
    10081 acgctctcaa aaagtttaat tgtcatgata aatcttctgc cttaataatt ggggatagct
    10141 taacctctga tgtcttaggt ggaataaatt caggaataga tacttgttgg ttaaattcca
    10201 ataacagtat aaattatact aatcatatac caacttatga aataaataca cttattgaat
    10261 taaagaaact actatagttt gtaagtctgt tcaaattaaa tagaatatta ctttgcaata
    10321 atttttttag tttatacata aaatgaccaa aaaaattatt gcaatttata cacaaaacaa
    10381 atcttagtct tgaaaattaa ctaaaagcat tttctaggac acttactaca tcttgattct
    10441 aaattttctt catcccccat taaaataagc attgcaacac tttttatggg ggatagttga
    10501 tgataatcat taatttctat ttctatacta ttatatttat tttttacata gttataaata
    10561 tccttttgtc tctctacttc aaaatcttta tctcctggat atatttcatg cgatattttg
    10621 agactatcat actttccttt taaatacatt tttattttct cattaatata atctaaacat
    10681 actataccta ttttatccaa tgctagccct gccatcatat tgcaattttc catatagtca
    10741 ttcatcttaa tatcaatttt atcccctatt gtatatagta cagcaaatgc ttttttacat
    10801 tctttaggtg ttttattaat atcaacttca cttatatata tcttgatatc taaaatatca
    10861 tttatatttc ttatttctct ttcaacgatt ttactaattg tatctggagc ttttctattt
    10921 ccatacccca aaaactttag cacaatattc ttatctataa aaatatcatc taaatttata
    10981 tcctttaaaa gatatttatt tttattacta ttataattca tcaatatcaa tcctatctaa
    11041 ttctttataa ctaattcttc ctgcctttat agctgcttgt aaattttcta ctggagtggt
    11101 atttaagcag ttacagccaa aatcacataa aaagttatca tatcttctca tcttctttaa
    11161 caattttagc gtctctcttc taatcttatc tggcttagaa cgtccaagaa gttttattgg
    11221 gtctaaatta ccaatcaaaa ctatatctct aggaatcatt ggagcaattt cttcatagtc
    11281 taaaatttgg tctaaactta ctgcatctat attacaactt agcatattat ctaataattc
    11341 tctggtgtct ccacatatat gcataccttt ccaacaatca agttcattgt agattttatt
    11401 taaatttttt actataaatt catcaaatct atctttactt agggttactg atgttggctc
    11461 tgctattgaa atatattttg ctcctgcttt atttgcagct acagcatatc ttcttaccaa
    11521 ttcagttgta aattccaata attcttctac aaatttttta tcagttatta tacatcttaa
    11581 aagttgagta gctccagcta attgacctgc aagtgtaaat ggaccttgta tcgattcata
    11641 tagtggtttt ttaatacttt ttgatataac tgataagctc tctatattga gtggcatacg
    11701 tccactcgta taagggtctg gaacatctaa ttttcttaat ttatctctgt cagttatagt
    11761 atgtgtcaat acacttggaa aatcataatc tggtcttaat gtctctaaac ccagagtttc
    11821 acaaaatgta atcccatcac agaatgagta tataaaatct gattcaaaat acttgtccat
    11881 agtctttgct aattctaatt gcaaatctac actatcatat acttcatatg cctttttatt
    11941 tattaagaac aatccatttg tacccatatc aggcagaaaa aaacctctat cttcactatt
    12001 tatatactga agtaaagtca tatctgaaaa catttaaact tcacctccta taagtttatt
    12061 tattaattta actgcttttg gagcatttga actgtatcca tctgctccta tttcttctgc
    12121 aaactccata cttacaggtc ctccaccaat tattactttt ggtcttttat ctatctcaat
    12181 catattaagt tcgtctataa ccgatttcat attttccatt gtagttgtca tcattgaaga
    12241 taagcctatt atatctacat tgttttttat agcttcttct attatatctt cactttttct
    12301 atttacacct aagtcaataa ccttatatcc agtggcttct accattactt ttacaatatt
    12361 ttttcctatc tcatgagtat ctccttctac tacagacatc agaattactc cctttgattt
    12421 tttctttact tttccagttc ccttcaaaag attaatacct ttatttaatg catctgtgca
    12481 tactataact tctgaaagat agtattcctt attctcaaac ttatctaaag ctcctatcat
    12541 acctttgttt aaaccatatt cataaatatc ttccacatca atatttttat ctatagcaat
    12601 ttttactaaa ttctctatat tatctacacc catatttaat atacattctg ctatatcatc
    12661 aagtatgtcc atataatact catcctttct aattacttac aaaattgctt tttcaatatc
    12721 caataatttc tcttatttaa caaggactaa ctctaggtat caatcaattt tatttaatat
    12781 catctaaaac tattgataat ttaaatttgc agtccatgga aaatctacag gaaatgcctt
    12841 tgtagcaaat tcatatatat tttttagatg tttaaattca ttgtactgtt cctcattttt
    12901 ttcaaaaaac agtttattaa gcttatccaa attttctatt acaagttctt cttctttgta
    12961 tttaataata tatttatttt caacttgttt aaattccatg tattttagta attcaaaatc
    13021 tacatattga ctaaaataac ttcttaaact atcacacaac ttagtaaaat ttattatttt
    13081 catagttcct tgttgataat ccaaagattt tgtcccatta catttcaact ggtctttaag
    13141 atttttcaca tgtactttat aatttaaata ttctaatcca aatttatttg caacatattt
    13201 caatatattt tcaacattta cagagtttaa ccctatttca gcaacttctc ctacagtaga
    13261 atcttctttt tttattgttc ttattatcaa atatcctatt atattattat tttcctttat
    13321 aaataccttc ttgtaaccaa ttggtcccca ggcaatagtt gctgcatgca atagtttttg
    13381 aaactcatct ctcgttctta taaatctagt actattttga ttatatagtt caatcatttt
    13441 atctaaatca ttatcttttt caaaattagt ttcatcaaat tcaactattt catatgctat
    13501 ttttacatct tcaggtttta ttgtatattt ataaaaactc tttaccaaac tacaatttct
    13561 tctactgtat agacttcttg ttcctgatat aattacaata tctacaccat cataaaacat
    13621 tttttcttct actttatcca atatcttaga tgaatagcct ctcttttcat aatctggatg
    13681 agtacatact ccacctatag ctgcaacctt caatctgttt ccttgaatag atacatcctg
    13741 aattagatag tttacatcag aaatcacttt atcatcttct gatattataa tcatattttc
    13801 tatattattt ttacttagta aaagaggaaa ttcttccatc atagttggtt tatgatttct
    13861 tgatattcta aatacatagt ttattaattc tattacttta tcaaattctt ctaattttgc
    13921 acttcttggt cttaattcca ttcatattcc tccaaatatt atttaagata cttaaaactc
    13981 tattatctat aataaaacat ttattactac agttctaatt atatcttttt tctttacaa
//
LOCUS       DAELTA010000099        13960 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.099, whole genome shotgun sequence.
ACCESSION   DAELTA010000099 DAELTA010000000
VERSION     DAELTA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.099"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            92..646
                     /gene="tcdR"
                     /locus_tag="KPG13_002483"
     CDS             92..646
                     /gene="tcdR"
                     /locus_tag="KPG13_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG0986193.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            964..8064
                     /gene="tcdB"
                     /locus_tag="KPG13_002484"
                     /gene_synonym="toxB"
     CDS             964..8064
                     /gene="tcdB"
                     /locus_tag="KPG13_002484"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG0986194.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8186..8686
                     /gene="tcdE"
                     /locus_tag="KPG13_002485"
     CDS             8186..8686
                     /gene="tcdE"
                     /locus_tag="KPG13_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG0986195.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8991..9197
                     /locus_tag="KPG13_002486"
     CDS             8991..9197
                     /locus_tag="KPG13_002486"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986196.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9414..>13960
                     /gene="tcdA"
                     /locus_tag="KPG13_002487"
                     /gene_synonym="toxA"
     CDS             9414..>13960
                     /gene="tcdA"
                     /locus_tag="KPG13_002487"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0986197.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINV"
BASE COUNT         5664 a         1443 c         2211 g         4642 t
ORIGIN      
        1 ccgattatat aattataatg actgatttaa ttccaatgtt gtcaaaattt tcaaataaat
       61 catcattata aatataagag aggatgattt tatgcaaaag tctttttatg aattaattgt
      121 tttagcaaga aataactcag tagatgattt gcaagaaatt ttatttatgt ttaagccatt
      181 agtaaaaaaa cttagtagag ttttacatta tgaagaggga gaaacagatt taataatatt
      241 ttttattgaa ttaataaaaa atattaaatt aagtagcttt tcagaaaaaa gcgatgctat
      301 tatagtcaaa tatattcata aatcattact gaataagact tttgagttgt ctagaagata
      361 ttctaaaatg aagtttaatt ttgtagaatt tgatgaaaat atcttaaata tgaaaaataa
      421 ttatcaaagt aagtctattt ttgaggaaga tatttgtttt ttcggatata ttttgaaaga
      481 attatctggt attcaaagaa aagttatttt ttataaatat ttaaaaggat attctgatag
      541 agaaatatca gtgaaattaa aaatatctag acaagctgtt aataaggcta aaaatagagc
      601 atttaaaaaa ataaaaaaag actatgaaaa ttattttaac ttgtaattaa tgagtttaaa
      661 gaaatattta caatagaaat caaattttat aattaacttt attgtaaaat taataactta
      721 atctaagaat atcttaattt ttatatttta tatagaacaa tgtttacata tttatttcag
      781 acaacgtctt tattcaatcg aagagcaaat taatcaactg agtgtcttaa atttaaaatg
      841 ttaggaagtg aatgtatatg aaaacctaag tagatattag tatattttat aaatagaaag
      901 gaggatatat aaaagagttt tagcatttag atgtaaaaat attatagtaa aggagaaaat
      961 tttatgagtt tagttaatag aaaacagtta gaaaaaatgg caaatgtaag atttcgtact
     1021 caagaagatg aatatgttgc aatattggat gctttagaag aatatcataa tatgtcagag
     1081 aatactgtag tcgaaaaata tttaaaatta aaagatataa atagtttaac agatatttat
     1141 atagatacat ataaaaaatc tggtagaaat aaagccttaa aaaaatttaa ggaatatcta
     1201 gttacagaag tattagagct aaagaataat aatttaactc cagttgagaa aaatttacat
     1261 tttgtttgga ttggaggtca aataaatgac actgctatta attatataaa tcaatggaaa
     1321 gatgtaaata gtgattataa tgttaatgtt ttttatgata gtaatgcatt tttgataaac
     1381 acattgaaaa aaactgtagt agaatcagca ataaatgata cacttgaatc atttagagaa
     1441 aacttaaatg accctagatt tgactataat aaattcttca gaaaacgtat ggaaataatt
     1501 tatgataaac agaaaaattt cataaactac tataaagctc aaagagaaga aaatcctgaa
     1561 cttataattg atgatattgt aaagacatat ctttcaaatg agtattcaaa ggagatagat
     1621 gaacttaata cctatattga agaatcctta aataaaatta cacagaatag tggaaatgat
     1681 gttagaaact ttgaagaatt taaaaatgga gagtcattca acttatatga acaagagttg
     1741 gtagaaaggt ggaatttagc tgctgcttct gacatattaa gaatatctgc attaaaagaa
     1801 attggtggta tgtatttaga tgttgatatg ttaccaggaa tacaaccaga cttatttgag
     1861 tctatagaga aacctagttc agtaacagtg gatttttggg aaatgacaaa gttagaagct
     1921 ataatgaaat acaaagaata tataccagaa tatacctcag aacattttga catgttagac
     1981 gaagaagttc aaagtagttt tgaatctgtt ctagcttcta agtcagataa atcagaaata
     2041 ttctcatcac ttggtgatat ggaggcatca ccactagaag ttaaaattgc atttaatagt
     2101 aagggtatta taaatcaagg gctaatttct gtgaaagact catattgtag caatttaata
     2161 gtaaaacaaa tcgagaatag atataaaata ttgaataata gtttaaatcc agctattagc
     2221 gaggataatg attttaatac tacaacgaat acctttattg atagtataat ggctgaagct
     2281 aatgcagata atggtagatt tatgatggaa ctaggaaagt atttaagagt tggtttcttc
     2341 ccagatgtta aaactactat taacttaagt ggccctgaag catatgcggc agcttatcaa
     2401 gatttattaa tgtttaaaga aggcagtatg aatatccatt tgatagaagc tgatttaaga
     2461 aactttgaaa tctctaaaac taatatttct caatcaactg aacaagaaat ggctagctta
     2521 tggtcatttg acgatgcaag agctaaagct caatttgaag aatataaaag gaattatttt
     2581 gaaggttctc ttggtgaaga tgataatctt gatttttctc aaaatatagt agttgacaag
     2641 gagtatcttt tagaaaaaat atcttcatta gcaagaagtt cagagagagg atatatacac
     2701 tatattgttc agttacaagg agataaaatt agttatgaag cagcatgtaa cttatttgca
     2761 aagactcctt atgatagtgt actatttcag aaaaatatag aagattcaga aattgcatat
     2821 tattataatc ctggagatgg tgaaatacaa gaaatagaca agtataaaat tccaagtata
     2881 atttctgata gacctaagat taaattaaca tttattggtc atggtaaaga tgaatttaat
     2941 actgatatat ttgcaggttt tgatgtagat tcattatcca cagaaataga agcagcaata
     3001 gatttagcta aagaggatat ttctcctaag tcaatagaaa taaatttatt aggatgtaat
     3061 atgtttagct actctatcaa cgtagaggag acttatcctg gaaaattatt acttaaagtt
     3121 aaagataaaa tatcagaatt aatgccatct ataagtcaag actctattat agtaagtgca
     3181 aatcaatatg aagttagaat aaatagtgaa ggaagaagag aattattgga tcattctggt
     3241 gaatggataa ataaagaaga aagtattata aaggatattt catcaaaaga atatatatca
     3301 tttaatccta aagaaaataa aattacagta aaatctaaaa atttacctga gctatctaca
     3361 ttattacaag aaattagaaa taattctaat tcaagtgata ttgaactaga agaaaaagta
     3421 atgttaacag aatgtgagat aaatgttatt tcaaatatag atacgcaaat tgttgaggaa
     3481 aggattgaag aagctaagaa tttaacttct gactctatta attatataaa agatgaattt
     3541 aaactaatag aatctatttc tgatgcacta tgtgacttaa aacaacagaa tgaattagaa
     3601 gattctcatt ttatatcttt tgaggacata tcagagactg atgagggatt tagtataaga
     3661 tttattaata aagaaactgg agaatctata tttgtagaaa ctgaaaaaac aatattctct
     3721 gaatatgcta atcatataac tgaagagatt tctaagataa aaggtactat atttgatact
     3781 gtaaatggta agttagtaaa aaaagtaaat ttagatacta cacacgaagt aaatacttta
     3841 aatgctgcat tttttataca atcattaata gaatataata gttctaaaga atctcttagt
     3901 aatttaagtg tagcaatgaa agtccaagtt tacgctcaat tatttagtac tggtttaaat
     3961 actattacag atgcagccaa agttgttgaa ttagtatcaa ctgcattaga tgaaactata
     4021 gacttacttc ctacattatc tgaaggatta cctataattg caactattat agatggtgta
     4081 agtttaggtg cagcaatcaa agagctaagt gaaacgagtg acccattatt aagacaagaa
     4141 atagaagcta agataggtat aatggcagta aatttaacaa cagctacaac tgcaatcatt
     4201 acttcatctt tgggggtagc tagtggattt agtatacttt tagttccttt agcaggaatt
     4261 tcagcaggta taccaagctt agtaaacaat gaacttgtac ttcgagataa ggcaacaaag
     4321 gttgtagatt attttaaaca tgtttcatta gttgaaactg aaggagtatt tactttatta
     4381 gatgataaaa taatgatgcc acaagatgat ttagtgatat cagaaataga ttttaataat
     4441 aattcaatag ttttaggtaa atgtgaaatc tggagaatgg aaggtggttc aggtcatact
     4501 gtaactgatg atatagatca cttcttttca gcaccatcaa taacatatag agagccacac
     4561 ttatctatat atgacgtatt ggaagtacaa aaagaagaac ttgatttgtc aaaagattta
     4621 atggtattac ctaatgctcc aaatagagta tttgcttggg aaacaggatg gacaccaggt
     4681 ttaagaagct tagaaaatga tggcacaaaa ctgttagacc gtataagaga taactatgaa
     4741 ggtgagtttt attggagata ttttgctttt atagctgatg ctttaataac aacattaaaa
     4801 ccaagatatg aagatactaa tataagaata aatttagata gtaatactag aagttttata
     4861 gttccaataa taactacaga atatataaga gaaaaattat catattcttt ctatggttca
     4921 ggaggaactt atgcattgtc tctttctcaa tataatatgg gtataaatat agaattaagt
     4981 gaaagtgatg tttggattat agatgttgat aatgttgtga gagatgtaac tatagaatct
     5041 gataaaatta aaaaaggtga tttaatagaa ggtattttat ctacactaag tattgaagag
     5101 aataaaatta tcttaaatag ccatgagatt aatttttctg gtgaggtaaa tggaagtaat
     5161 ggatttgttt ctttaacatt ttcaatttta gaaggaataa atgcaattat agaagttgat
     5221 ttattatcta aatcatataa attacttatt tctggcgaat taaaaatatt gatgttaaat
     5281 tcaaatcata ttcaacagaa aatagattat ataggattca atagcgaatt acagaaaaat
     5341 ataccatata gctttgtaga tagtgaagga aaagagaatg gttttattaa tggttcaaca
     5401 aaagaaggtt tatttgtatc tgaattacct gatgtagttc ttataagtaa ggtttatatg
     5461 gatgatagta agccttcatt tggatattat agtaataatt tgaaagatgt caaagttata
     5521 actaaagata atgttaatat attaacaggt tattatctta aggatgatat aaaaatctct
     5581 ctttctttga ctctacaaga tgaaaaaact ataaagttaa atagtgtgca tttagatgaa
     5641 agtggagtag ctgagatttt gaagttcatg aatagaaaag gtaatacaaa tacttcagat
     5701 tctttaatga gctttttaga aagtatgaat ataaaaagta ttttcgttaa tttcttacaa
     5761 tctaatatta agtttatatt agatgctaat tttataataa gtggtactac ttctattggc
     5821 caatttgagt ttatttgtga tgaaaatgat aatatacaac catatttcat taagtttaat
     5881 acactagaaa ctaattatac tttatatgta ggaaatagac aaaatatgat agtggaacca
     5941 aattatgatt tagatgattc tggagatata tcttcaactg ttatcaattt ctctcaaaag
     6001 tatctttatg gaatagacag ttgtgttaat aaagttgtaa tttcaccaaa tatttataca
     6061 gatgaaataa atataacgcc tgtatatgaa acaaataata cttatccaga agttattgta
     6121 ttagatgcaa attatataaa tgaaaaaata aatgttaata tcaatgatct atctatacga
     6181 tatgtatgga gtaatgatgg taatgatttt attcttatgt caactagtga agaaaataag
     6241 gtgtcacaag ttaaaataag attcgttaat gtttttaaag ataagacttt ggcaaataag
     6301 ctatctttta actttagtga taaacaagat gtacctgtaa gtgaaataat cttatcattt
     6361 acaccttcat attatgagga tggattgatt ggctatgatt tgggtctagt ttctttatat
     6421 aatgagaaat tttatattaa taactttgga atgatggtat ctggattaat atatattaat
     6481 gattcattat attattttaa accaccagta aataatttga taactggatt tgtgactgta
     6541 ggcgatgata aatactactt taatccaatt aatggtggag ctgcttcaat tggagagaca
     6601 ataattgatg acaaaaatta ttatttcaac caaagtggag tgttacaaac aggtgtattt
     6661 agtacagaag atggatttaa atattttgcc ccagctaata cacttgatga aaacctagaa
     6721 ggagaagcaa ttgattttac tggaaaatta attattgacg aaaatattta ttattttgat
     6781 gataattata gaggagctgt agaatggaaa gaattagatg gtgaaatgca ctattttagc
     6841 ccagaaacag gtaaagcttt taaaggtcta aatcaaatag gtgattataa atactatttc
     6901 aattctgatg gagttatgca aaaaggattt gttagtataa atgataataa acactatttt
     6961 gatgattctg gtgttatgaa agtaggttac actgaaatag atggcaagca tttctacttt
     7021 gctgaaaacg gagaaatgca aataggagta tttaatacag aagatggatt taaatatttt
     7081 gctcatcata atgaagattt aggaaatgaa gaaggtgaag aaatctcata ttctggtata
     7141 ttaaatttca ataataaaat ttactatttt gatgattcat ttacagctgt agttggatgg
     7201 aaagatttag aggatggttc aaagtattat tttgatgaag atacagcaga agcatatata
     7261 ggtttgtcat taataaatga tggtcaatat tattttaatg atgatggaat tatgcaagtt
     7321 ggatttgtca ctataaatga taaagtcttc tacttctctg actctggaat tatagaatct
     7381 ggagtacaaa acatagatga caattatttc tatatagatg ataatggtat agttcaaatt
     7441 ggtgtatttg atacttcaga tggatataaa tattttgcac ctgctaatac tgtaaatgat
     7501 aatatttacg gacaagcagt tgaatatagt ggtttagtta gagttgggga agatgtatat
     7561 tattttggag aaacatatac aattgagact ggatggatat atgatatgga aaatgaaagt
     7621 gataaatatt atttcaatcc agaaactaaa aaagcatgca aaggtattaa tttaattgat
     7681 gatataaaat attattttga tgagaagggc ataatgagaa cgggtcttat atcatttgaa
     7741 aataataatt attactttaa tgagaatggt gaaatgcaat ttggttatat aaatatagaa
     7801 gataagatgt tctattttgg tgaagatggt gtcatgcaga ttggagtatt taatacacca
     7861 gatggattta aatactttgc acatcaaaat actttggatg agaattttga gggagaatca
     7921 ataaactata ctggttggtt agatttagat gaaaagagat attattttac agatgaatat
     7981 attgcagcaa ctggttcagt tattattgat ggtgaggagt attattttga tcctgataca
     8041 gctcaattag tgattagtga atagataaaa atatgttaaa tatatcctct tatacttaaa
     8101 tatataaaaa taaacaaaat gatacactac ataaagtgtt ctatctaata tgaagattta
     8161 ccaataaaaa ggtggactat gatgaatgca cagtagttca cctttttata tttctaatgg
     8221 taacaaaata tttttttata taaacctagg aggcgttatg aatatgacaa tatctttttt
     8281 atcagagcat atatttataa agttagtaat tttaactata tcatttgata cattattagg
     8341 atgtttaagt gcaataaaaa gtcgtaaatt taattctagt tttggaatag atggaggaat
     8401 cagaaaagta gcaatgatag catgtatatt ttttttatca gtagttgaca ttcttacaaa
     8461 gtttaacttt ttatttatgt taccacaaga ttgtatcaat tttttaagac taaaacatct
     8521 tggaatatct gaatttttct ctattttatt tattttatat gaaagtgtaa gtatattaaa
     8581 aaatatgtgc ttatgtggat taccagtacc taagagatta aaggaaaaaa tagcaatttt
     8641 actagatgca atgacagatg aaatgaatgc taaggatgaa aagtaagtaa tggtagatat
     8701 aataaagata ttaacaaata aaaagtgtta tccaaataag aatagctgaa agttatcata
     8761 attcatgaaa ctaataatga aaacgaggga gcagatgcca agagacacac aagtattaaa
     8821 tacatataat ttcgaagcaa gtgttcatta ctatatagat gacaaggtag tatatcaaac
     8881 attggttcac aaagatggtg catggtcagt tggtaaaatc tattaagcta cattagttac
     8941 agatatcaca aactataata gttaaacata gaaatatgtg taaattgtga ttgaaattat
     9001 tcaaaaacac aaaaatacgt gatgaaggac aaaatgatat agaaaataag tatcaaacct
     9061 taataaatga tttaattgat agtttaaaag ttataggaaa aatatataaa gaaataaaaa
     9121 cattaaaaaa atataagata tgtttacaaa ttactatcag acaatctcct tatctaatag
     9181 aagagtcaat taactaattg agtatcttta aattgaaatg ttaggaagtg atttaaatat
     9241 gaaaacttaa attataaaaa atcaatatta atttattttt aaaaaataga aaggagtgta
     9301 taagatttat tttcaaagtt taaaaacaag aaaatcaatt taaatttcag aaggaataaa
     9361 tgtggttata gaagtggatt tattatcaaa aataataata ctaggaggtt tttatgtctt
     9421 taatatctaa agaagagtta ataaaactcg catatagcat tagaccaaga gaaaatgagt
     9481 ataaaactat actaactaat ttagacgaat ataataagtt aactacaaac aataatgaaa
     9541 ataaatattt acaattaaaa aaactaaatg aatcaattga tgtttttatg aataaatata
     9601 aaacttcaag cagaaataga gcactctcta atctaaaaaa agatatatta aaagaagtaa
     9661 ttcttattaa aaattccaat acaagccctg tagaaaaaaa tttacatttt gtatggatag
     9721 gtggagaagt cagtgatatt gctcttgaat acataaaaca atgggctgat attaatgcag
     9781 aatataatat taaactgtgg tatgatagtg aagcattctt agtaaataca ctaaaaaagg
     9841 ctatagttga atcttctacc actgaagcat tacagctact agaggaagag attcaaaatc
     9901 ctcaatttga taatatgaaa ttttacaaaa aaaggatgga atttatatat gatagacaaa
     9961 aaaggtttat aaattattat aaatctcaaa tcaataaacc tacagtacct acaatagatg
    10021 atattataaa gtctcatcta gtatctgaat ataatagaga tgaaactgta ttagaatcat
    10081 atagaacaaa ttctttgaga aaaataaata gtaatcatgg gatagatatc agggctaata
    10141 gtttgtttac agaacaagag ttattaaata tttatagtca ggagttgtta aatcgtggaa
    10201 atttagctgc agcatctgac atagtaagat tattagccct aaaaaatttt ggcggagtat
    10261 atttagatgt tgatatgctt ccaggtattc actctgattt atttaaaaca atatctagac
    10321 ctagctctat tggactagac cgttgggaaa tgataaaatt agaggctatt atgaagtata
    10381 aaaaatatat aaataattat acatcagaaa actttgataa acttgatcaa caattaaaag
    10441 ataattttaa actcattata gaaagtaaaa gtgaaaaatc tgagatattt tctaaattag
    10501 aaaatttaaa tgtatctgat cttgaaatta aaatagcttt cgctttaggc agtgttataa
    10561 atcaagcctt gatatcaaaa caaggttcat atcttactaa cctagtaata gaacaagtaa
    10621 aaaatagata tcaattttta aaccaacacc ttaacccagc catagagtct gataataact
    10681 tcacagatac tactaaaatt tttcatgatt cattatttaa ttcagctacc gcagaaaact
    10741 ctatgttttt aacaaaaata gcaccatact tacaagtagg ttttatgcca gaagctcgct
    10801 ccacaataag tttaagtggt ccaggagctt atgcgtcagc ttactatgat ttcataaatt
    10861 tacaagaaaa tactatagaa aaaactttaa aagcatcaga tttaatagaa tttaaattcc
    10921 cagaaaataa tctatctcaa ttgacagaac aagaaataaa tagtctatgg agctttgatc
    10981 aagcaagtgc aaaatatcaa tttgagaaat atgtaagaga ttatactggt ggatctcttt
    11041 ctgaagacaa tggggtagac tttaataaaa atactgccct cgacaaaaac tatttattaa
    11101 ataataaaat tccatcaaac aatgtagaag aagctggaag taaaaattat gttcattata
    11161 tcatacagtt acaaggagat gatataagtt atgaagcaac atgcaattta ttttctaaaa
    11221 atcctaaaaa tagtattatt atacaacgaa atatgaatga aagtgcaaaa agctactttt
    11281 taagtgatga tggagaatct attttagaat taaataaata taggatacct gaaagattaa
    11341 aaaataagga aaaagtaaaa gtaaccttta ttggacatgg taaagatgaa ttcaacacaa
    11401 gcgaatttgc tagattaagt gtagattcac tttccaatga gataagttca tttttagata
    11461 ccataaaatt agatatatca cctaaaaatg tagaagtaaa cttacttgga tgtaatatgt
    11521 ttagttatga ttttaatgtt gaagaaactt atcctgggaa gttgctatta agtattatgg
    11581 acaaaattac ttccacttta cctgatgtaa ataaaaattc tattactata ggagcaaatc
    11641 aatatgaagt aagaattaat agtgagggaa gaaaagaact tctggctcac tcaggtaaat
    11701 ggataaataa agaagaagct attatgagcg atttatctag taaagaatac attttttttg
    11761 attctataga taataagcta aaagcaaagt ccaagaatat tccaggatta gcatcaatat
    11821 cagaagatat aaaaacatta ttacttgatg caagtgttag tcctgataca aaatttattt
    11881 taaataatct taagcttaat attgaatctt ctattggtga ttacatttat tatgaaaaat
    11941 tagagcctgt taaaaatata attcacaatt ctatagatga tttaatagat gagttcaatc
    12001 tacttgaaaa tgtatctgat gaattatatg aattaaaaaa attaaataat ctagatgaga
    12061 agtatttaat atcttttgaa gatatctcaa aaaataattc aacttactct gtaagattta
    12121 ttaacaaaag taatggtgag tcagtttatg tagaaacaga aaaagaaatt ttttcaaaat
    12181 atagcgaaca tattacaaaa gaaataagta ctataaagaa tagtataatt acagatgtta
    12241 atggtaattt attggataat atacagttag atcatacttc tcaagttaat acattaaacg
    12301 cagcattctt tattcaatca ttaatagatt atagtagcaa taaagatgta ctgaatgatt
    12361 taagtacctc agttaaggtt caactttatg ctcaactatt tagtacaggt ttaaatacta
    12421 tatatgactc tatccaatta gtaaatttaa tatcaaatgc agtaaatgat actataaatg
    12481 tactacctac aataacagag gggataccta ttgtatctac tatattagac ggaataaact
    12541 taggtgcagc aattaaggaa ttactagacg aacatgaccc attactaaaa aaagaattag
    12601 aagctaaggt gggtgtttta gcaataaata tgtcattatc tatagctgca actgtagctt
    12661 caattgttgg aataggtgct gaagttacta ttttcttatt acctatagct ggtatatctg
    12721 caggaatacc ttcattagtt aataatgaat taatattgca tgataaggca acttcagtgg
    12781 taaactattt taatcatttg tctgaatcta aaaaatatgg ccctcttaaa acagaagatg
    12841 ataaaatttt agttcctatt gatgatttag taatatcaga aatagatttt aataataatt
    12901 cgataaaact aggaacatgt aatatattag caatggaggg gggatcagga cacacagtga
    12961 ctggtaatat agatcacttt ttctcatctc catctataag ttctcatatt ccttcattat
    13021 caatttattc tgcaataggt atagaaacag aaaatctaga tttttcaaaa aaaataatga
    13081 tgttacctaa tgctccttca agagtgtttt ggtgggaaac tggagcagtt ccaggtttaa
    13141 gatcattgga aaatgacgga actagattac ttgattcaat aagagattta tacccaggta
    13201 aattttactg gagattctat gcttttttcg attatgcaat aactacatta aaaccagttt
    13261 atgaagacac taatattaaa attaaactag ataaagatac tagaaacttc ataatgccaa
    13321 ctataactac taacgaaatt agaaacaaat tatcttattc atttgatgga gcaggaggaa
    13381 cttactcttt attattatct tcatatccaa tatcaacgaa tataaattta tctaaagatg
    13441 atttatggat atttaatatt gataatgaag taagagaaat atctatagaa aatggtacta
    13501 ttaaaaaagg aaagttaata aaagatgttt taagtaaaat tgatataaat aaaaataaac
    13561 ttattatagg caatcaaaca atagattttt caggcgatat agataataaa gatagatata
    13621 tattcttgac ttgtgagtta gatgataaaa ttagtttaat aatagaaata aatcttgttg
    13681 caaaatctta tagtttgtta ttgtctgggg ataaaaatta tttgatatcc aatttatcta
    13741 atactattga gaaaatcaat actttaggcc tagatagtaa aaatatagcg tacaattaca
    13801 ctgatgaatc taataataaa tattttggag ctatatctaa aacaagtcaa aaaagcataa
    13861 tacattataa aaaagacagt aaaaatatat tagaatttta taatgacagt acattagaat
    13921 ttaacagtaa agattttatt gctgaagata taaatgtatt
//
LOCUS       DAELTA010000100        13694 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.100, whole genome shotgun sequence.
ACCESSION   DAELTA010000100 DAELTA010000000
VERSION     DAELTA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.100"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(146..607)
                     /locus_tag="KPG13_002488"
     CDS             complement(146..607)
                     /locus_tag="KPG13_002488"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG0986198.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(712..1905)
                     /locus_tag="KPG13_002489"
     CDS             complement(712..1905)
                     /locus_tag="KPG13_002489"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG0986199.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(1928..2593)
                     /locus_tag="KPG13_002490"
     CDS             complement(1928..2593)
                     /locus_tag="KPG13_002490"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG0986200.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(2627..3739)
                     /gene="ribD"
                     /locus_tag="KPG13_002491"
     CDS             complement(2627..3739)
                     /gene="ribD"
                     /locus_tag="KPG13_002491"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG0986201.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3871..3992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4181..6664
                     /gene="recQ"
                     /locus_tag="KPG13_002492"
     CDS             4181..6664
                     /gene="recQ"
                     /locus_tag="KPG13_002492"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG0986202.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6753..6864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7006..8307
                     /gene="thiC"
                     /locus_tag="KPG13_002493"
     CDS             7006..8307
                     /gene="thiC"
                     /locus_tag="KPG13_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG0986203.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8361..8555
                     /gene="thiS"
                     /locus_tag="KPG13_002494"
     CDS             8361..8555
                     /gene="thiS"
                     /locus_tag="KPG13_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG0986204.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8548..9351
                     /gene="thiF"
                     /locus_tag="KPG13_002495"
     CDS             8548..9351
                     /gene="thiF"
                     /locus_tag="KPG13_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG0986205.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            9414..10184
                     /locus_tag="KPG13_002496"
     CDS             9414..10184
                     /locus_tag="KPG13_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG0986206.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10201..11304
                     /gene="thiH"
                     /locus_tag="KPG13_002497"
     CDS             10201..11304
                     /gene="thiH"
                     /locus_tag="KPG13_002497"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG0986207.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11305..11994
                     /locus_tag="KPG13_002498"
     CDS             11305..11994
                     /locus_tag="KPG13_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG0986208.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12182..13576
                     /gene="yfcC"
                     /locus_tag="KPG13_002499"
     CDS             12182..13576
                     /gene="yfcC"
                     /locus_tag="KPG13_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG0986209.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         5121 a         1666 c         2288 g         4619 t
ORIGIN      
        1 attaatttat attactggaa tttttaattc cagtaatatt tttttattat ataatttagc
       61 tttattaaaa gtttttatta ttaaaaatag cttatttatc gtaaagtgaa tgatattggg
      121 ttttaattat tatgaatgtt agtatttaat ttaaagactt aaataaattt gccatttcta
      181 gcgcattcat agcacaatca taacctttat ttccagcttt tgtacctgct ctttcaatag
      241 cttgttcaat attttctgta gttacgattc caaatataac aggtaattca ctgtctaaag
      301 atactttggc tatcccttta gagacttcac tacatacata atcataatga gaagtagccc
      361 ctcttataac acagcctaaa cagattatag catcatactt accactttta gccatttttt
      421 gagcaacaag aggaatctca aaagctccag gtacccaaac tatttctata ttttctggag
      481 aaacgtcatg tcttaaaaga cagtcttcag ctcctgataa aagttttgat gttataaatt
      541 cattaaaacg agaatttatt atacctattt ttaaatcttt tccaataagt tttccttcat
      601 atatcatttt tacttcctcc aattttattt atgttataga tttatattta taaatatctt
      661 taatttaatt ataaaaaact acttagtaga aaaatatcat catataatca tttatataat
      721 atctaaaaga tgtcccattt tatctttttt agtttttaaa tatagcttat taaagtcatt
      781 aatttcttca ttaataggga ttctattttc gatttttatt ccatatttct ctaattgatt
      841 tattttatct gggttattac ttaaaagatt tatacttttt atgtttaaat tttttaatat
      901 ttgagcagcc atatagaaat ctctcatatc ttcttcaaat ccaagcatta aatttgcttc
      961 aacagtatcg taaccttctt cttgaagttt ataagcctta attttattta aaagaccaat
     1021 acctctacct tcttgcttca tataaattaa aactccactt ccattttcca caattttctt
     1081 catagaagat tctagctgta atccacaatc acattttaga gagtggaaca catctcctgt
     1141 taaacattca gaatgaagtc taactagagt attttctata tttatatttc cataagtaag
     1201 ggcaatgtgt tcttcatttg aataagtatc tctatatcct atgatttcaa aatttccata
     1261 ttttgtcgga aggaaagcac tagatacttt ttctatagta gtttcattta ttttgcggta
     1321 ttctataagg tcttttatag tgatgatttt taaattatgc ttttctttga aaatcattaa
     1381 atcatcaact cttgccattg taccatcatc tttcattatt tcacagataa gaccaatctc
     1441 tttaaaacct gcaagtctag caaggtctac tgaggcttct gtatgtccat ccctgacaag
     1501 aaccccacca gtttttgcta ttaaaggaaa gatgtgacca ggcgttttga aatctttact
     1561 tgtagaattt tcatcacaga ataattttac agtttcagct ctttcatatg cagatatacc
     1621 agttagagta tctgctccat ctatagtaac agtaaaggct gtttgaaatg tatctgtatt
     1681 ttctctaacc ataagaggta tattaagact tttaaatttt ttttcagtgg caggtagaca
     1741 aattagacct ctaccatatg tagccataaa atttattgat tcaggagtag ccatttcagc
     1801 tgccatcaat aaatctcctt cattctctct actttcatca tcaaccacga ttaccatttt
     1861 cccatttttt atatcttcaa tagcttcttc aatagtatta aacatgtaat tcactccttt
     1921 attattttta aaaaccattt ttaaataaaa aatctttatc tatatttgat ttatggttat
     1981 tactattaga atttaattcc ttgtagttat tagccataaa attattaaca tactttccaa
     2041 ttacatcgct ttctaaatta acgaaatctc caacattctt tgtcaagaga atagtgttaa
     2101 ttttagtatg aggtataata gaaaccttaa atatttcatc atctaaatat gatatagtta
     2161 aacttacacc atcaatagca acagaacctt tgagtatcat tgaggataaa agttttttat
     2221 caggtcgtat acttactaaa actgcatttt catctttttc aaatttgcag atagttcctt
     2281 taccatcaac atgacctgtt actatatgac caccaaatct tgaagaaaga ctcatagctc
     2341 tttcaagatt taccttgtca tttgcattta ataaacctaa atttgtggaa cgaattgtct
     2401 ccatcataac atcggctgta aattcatttt ttccgaggtt tgaaacagtt agacaaactc
     2461 catttacggc tatactatct ccaagatttg tgccatctaa tattttattt gatagtatgg
     2521 ttacttttcc actcttacca ttagcagtaa tttttctaag tattccaact tcttctacta
     2581 tgccagtaaa catcctaacc tcctatatga atgtattaac aaaaatttaa tttaaaacat
     2641 ctgattctat aagaatatcg ccatctatca aagttacatt attgattgtt aactgtgttg
     2701 aatctgacaa atgatttatt ccttctccag aaacgaatgt aggtgcatct ttaccaccta
     2761 taatcttggg tgatagaaat aatttgacct tatcaactaa cttatttcta aagagactgt
     2821 cattgagagt agcaccacct tcaactagaa cagaatcaat gtttaactct cctaatttat
     2881 acattacaaa ctccaaatct actaagttgt tttttgaagg agaaattatt gtttcaacat
     2941 tattttcttt taacatagat aattttatac tttctttgca acatgtgaaa actatagttt
     3001 tactggtttt atctttaacc aaattactat taagtggcaa ctttaggtgt gtatctatta
     3061 ctattcttgt aggatgagac actttttctt gattgagcct acaagttagt aaaggattat
     3121 cgttcaaaac tgtatttatg ccaaccatta tactttggta tttatttctg tatttatgag
     3181 ttagatacct tgatgattca ttagatatcc attttgattc tagtgatttt gtagcaattt
     3241 taccatctag acttactgct gattttacaa tacaaagagg tcttttgttt tttatatgat
     3301 aaaaaaatgc ctcatttaaa tctcttgctt catcttctaa tattcccaca gtaacatcaa
     3361 tattgttatc ttttaatttt tttactccat taccacaaac aagtggattt gggtctaatg
     3421 tagatattac aactctctta atcttatttt gtataatttt atctacacaa ggaggcgttt
     3481 tcccataatg agaacatggt tctaagttta cataaagagt agagtctttt agactttgtt
     3541 tagcactgtt tatagcattt acttcagcat gattagagcc aaatttctca tgatatcctt
     3601 tgcctattat attactagct ttaacaatta cacacccaac taatggattt ggatttacaa
     3661 agccttctcc atttttagcc aattcaatag cttttttcat ataataaata tctttttctt
     3721 tttgattcac aaaattcaca atatgtacac ctcctgttta attggataat ttttaaacaa
     3781 aaaaccctga gaattaactc agggtttaga ataatctaca ataaatgttt aataaatgta
     3841 gaacaaaatt gctctacaaa aacactcatt aaatatcttc ttccatccag actttactgt
     3901 cggctttgga atttaaccaa atcaaccaag ttttaccttg gctcgcgggc tataccgccg
     3961 attgggaatt tcaccctacc ccgaagatta agtattaaat ttatatttag aatgtatcat
     4021 aaattatttt actttgcaaa ctattttttt aaaaaatata tgttaatata taatatagaa
     4081 tgtctataaa ctttgaaata ccacgattgt gaatataaaa ataaaaattt gaacataatc
     4141 aggaaagaag tctaattaat ttaaattagg agagtaatat atgaataaaa aacttttaga
     4201 aatactatca aaatactatg gatatacaag ttttagaaaa ggtcaagaat ctataataaa
     4261 ctctatctta agcgaaaaag atgtgttagc tattatgcca acaggagggg gaaaatcaat
     4321 atgttatcaa attccagctt tgattttaga tggaatgaca atagtaattt ctccacttat
     4381 atcacttatg aaagaccaag tagatgcctt aaagactatg ggaattagtg ctgcttttat
     4441 aaatagttca ttaagtagca aagaatttaa tgaaatatta aataatatta gaaaaaataa
     4501 ttataaaata ctctatattg ctccagagag attagatgca caagagttta ttgagctaat
     4561 aaataataac aatgtaagtc aagttgcgat agatgaagca cattgtgtat ctcaatgggg
     4621 gcatgatttt aggttgagtt atagaaggat ttctgatttt ataaaaaatt tgcctaaaag
     4681 acctatagtt acagcattta ctgcaacagc ttcagaagag gtaagaactg atataataaa
     4741 tttactgtgt ttagaaaatc cagattatta tataactgga tttgatagag agaatttatc
     4801 aataaatata gtaaaatcat ctagtaaaaa taagtatatt ttagattata ttcaaaatca
     4861 taaaaatgag tctggaatta tatatgttgc aactagaaag gaagttgaaa atatatataa
     4921 tggtttatct aagagaaata tatctgtgtc aaaatatcat gcaggtttat ctaaaaatga
     4981 aagaagtaaa aatcaagaag attttataaa tgataatgtg gatataatgg tagcaacaaa
     5041 tgcatttgga atgggtattg ataaacctaa tataagatgg gttatacatt acaatatgcc
     5101 tcaaagtata gaaaactact atcaagaaat aggtagagct ggtagggatg gagaaaaaag
     5161 tgagtgtgta cttttatttt ctccaggaga tgtacatata caaaaatatt taattgatat
     5221 tggagttgaa aatcctgaaa gaaaattatt tcagcataag aagcttcaat acatgattga
     5281 cttagtttac agtaattcat gctatagaaa gacaatattg agttattttg gagaaaatta
     5341 tatagacaat tgcaataatt gtagcaattg tttagtagag ggtgaaatag tagataaaac
     5401 tatagatgca caaaaagtca tttcatgtgt agctagaatg aaaagaagtt atggtgttac
     5461 tactattgta gatgtgctta gaggttctaa gaataaaaag attttacagc ttggattaaa
     5521 tactttgtct acatataaca ttatgagaga ttattctagt gaagacttaa agaattttgt
     5581 aaatacattg gtttctcatg gttttttaga tttagttgag actctaggca atggaaatag
     5641 aggtagtttt cctactataa gattaaatga gatgtctatg aaagttttaa aaggtgaagt
     5701 gaaagtagag tttaaagaaa ttgttatgac aaaatcattg gaagtagagg atgagttata
     5761 taatgcttta agagaactta gacattcaat tgcaagtgaa gaaaggatag ctccatatat
     5821 ggtatttgga gatggtacac ttagagccat gtctagtagt tatccaatta ataaagaaga
     5881 aatgctagat atctctggag taggagaaat taaatatcaa aaatatggta gagattttga
     5941 agcagtaatt aaagaatatg tcgaaaaaaa taatataaat aaaaaaatag atgaaaaaaa
     6001 tgtcgattca aagaacgtta atagtgattt cttagaggtt aatacagata aagttttata
     6061 tgaaaaactg ttagtcatta gagaagaata tgcaacaaag gaaaaattac ctgcatatat
     6121 gattttagcc aataaggctt tgaaagaaat aagtggaaga tatccacttg atgaagaaca
     6181 attaaaagat atttctggca taggtggagt aaaatttgaa aaatatggag ctagtatttt
     6241 ggcagaggtt aataaatatg taaaagaaaa taatattaat gttatttggg agaaaaaggg
     6301 caaaaggaag ttaatattag atggagaaag tagaaaaaat aatgaaatag cattagattt
     6361 gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa atagaagttt cactgtcaac
     6421 cattattgga tatgtgcatg actatgtaaa ggatggacat agtatatcat ttgaattagg
     6481 actagaaaat ttttataatg aagataaaaa gaagatgatt ttagaagttt gcgaaaaagt
     6541 tggctatgat aatttaaata acataaaaag aaaacttcca gattcaataa agtatgaaaa
     6601 tataagagct gtgatacttg actcttatct tgaaaactta aaaaataata ttatagcaga
     6661 gtaattttta acttaataaa aaataaaatt tgtattattg tgttgaacta ataattttgc
     6721 tgtgttatac tgaatttgta aaaataatga aaaattagag ctaggggcgc tagaattggc
     6781 tagctgagag ataaagccgt aacttacaaa ccctaataac ctgatcaaga taatgcttgc
     6841 gaagggaagc tttttaatat aattttatta tataaatagg caaattattc aatgttagga
     6901 gaaatttgtc taagtgtata tacgattata agagtgcttt ccaaacgagg aaaagcactt
     6961 tttttatttt caaaaactat aactttaatc ttggaggatt gtaatatgaa ctatacaaca
     7021 caaatggatg ctgctagaaa aggcataata actaaagaaa tggaaatcgt ttctcaaaaa
     7081 gaacaggtag atgtaaatga attaagagaa ttaatagcaa atggacaagt tgttatacca
     7141 gctaataaaa atcataaatc tttaagtgca gaaggtgtag gtaaaaattt aagaacaaaa
     7201 ataaatgtaa atctaggaat ttcaagagat tgtaaggata tagaaaaaga acttgaaaaa
     7261 gttagagtag caatagacat gaaagctgaa gctataatgg atttaagtaa ttatggcaaa
     7321 actagagaat ttagagaaaa ggttgttgaa atgtcacctg caatgatagg ttcagtacct
     7381 atgtatgatg cagtaggata tttggaaaaa gaattaaaag atataacaga agaagagttt
     7441 ttaaatgtaa taagacaaca tgctattgat ggagtagact ttattactat acatgcaggt
     7501 cttacaagaa gtgtttgcca aaaaataaaa aatcatgaga gattgactca tatagtatct
     7561 cgtggaggtt ctttattgtt tgcatggatg gaattaaata ataaggaaaa tcctatttat
     7621 actaattttg ataaaatatt ggatatatgt gaagaatatg atgttacatt gagtttagga
     7681 gatgcttgta gaccaggttg tataaaagat tctactgatg gtgttcaaat tcaagaactt
     7741 gtagtattag gtgaacttac taaaagagca tgggaaagaa atgtacaagt aatgatagaa
     7801 ggtcctggac acatggctat tgatgaaata gaagcaaatg tagtattaga aaagagatta
     7861 tgccatggag caccttttta tgttcttgga ccattggtta cagatatagc accaggttat
     7921 gaccatataa caagtgcaat aggaggagct ttagcatgtg ccaaaggagt agatttttta
     7981 tgttatgtaa ctccagcaga acatttaaga ttgcctaatt tagatgatat gaaagagggt
     8041 ataatagctg caaagatagc tgctcatgca ggagatatag ctaaaaatgt taaaggtgct
     8101 cgtgaatggg ataataagat gagtaaagct agagctgatt tagactggtg tgagatgttt
     8161 agacttgcaa tagaccctga aaaagctaag agatacagag atgaatcaac tcctactcat
     8221 gaggatagtt gtactatgtg tggaaaaatg tgctcaatga gaacagttaa gaaaatatta
     8281 aacaatgagg aacttaatct aatataattt ttggctagta tgactggaat aaaaataagc
     8341 tgaaaagagg aggtgattta gtgaaggtta atgggaagga aattgaattt gaaaaagatt
     8401 tgactgttat agatttatta aataaatata atttaaaaag tgatagagtt gtagttgaag
     8461 tgaatttaga gataattgaa gaatctaact acaatacata tgtattaaaa gacgaggaca
     8521 ttgtagagct tattagcttt attggaggtg gttaaaataa aaatatttgt aaatgaatta
     8581 ttaacagatg ttgaggaaaa tacaactgct tataaagtta gagatagcta taacaataaa
     8641 tgtgatgtat tggtgttaaa tggatatcca attaaaacag atatgaccct ttgtgaaaat
     8701 gataaattaa cacttataca aaaaggagtt aagccatcac ttgatgaatt ggaaagcttg
     8761 ttgattgcta gacatactcc aaatatacat aataaattaa aaaaagggaa agttgctata
     8821 ttgggattgg ggggacttgg ttccaatata gctatctctt tagctagaat aggagtggga
     8881 gaacttttac ttatagatta tgatgttgta gaaccatcta atttaaatag acagcagtat
     8941 tttatagatg atataggaaa actaaaaact gatgccatga tagaaaatat aaaaaaaata
     9001 aatccattta taaaattaaa taaacgagat atttttttaa acaagaacaa tatggatact
     9061 attaaggatt cagatttaat aattgaggcc tttgatgatg cttcatgtaa agcacaggta
     9121 tgtaattatg tattaataaa tttaaaagat aaatatttaa tagcatcatc tggtatggca
     9181 ggttactatg attcaaatat aataactact aagaagataa aagacaggtt ttatatatgt
     9241 ggggattttg tgaatgaagc taaatttggt gaagggctta tggctccaag ggttgccatc
     9301 tgcgcaaatc atatggcaaa tttggcaacg caaatactta tagatatgta aatattaaaa
     9361 ttagatataa aaaagattga atattaaaaa aatttaacgt ggagggaaat aatatggata
     9421 aattggtact tggtggacat gaatttaata gcagattatt ggttggaaca ggtaaatatg
     9481 gttcaaataa tatattgcct gaggtaataa aagaaagtgg aagtgaaata ataacaatgg
     9541 cacttagaag agtggattta gacaataaac aagaaaacat attaacgtat attccaaaag
     9601 agatgacaat acttccaaat acatcaggag ctactaatgc agaagaagct gtaagaatag
     9661 ctagaatatc tagaaaaatg ggatgtggag attttataaa aatagaagta atatcagata
     9721 ctagatattt acttcctgat aatgaagaaa ctataaaagc tacaaagata cttgctgatg
     9781 aggggtttat agttcttcca tatatgacac cagaccttta tgctggaaga agacttatag
     9841 aagctaatgc agcggcagtt atgccacttg gagctccaat tggttcaaat agaggtcttc
     9901 aaatgaaaga aatgataaga ataatgatag atgagttgga tataccaata atagtagatg
     9961 caggtattgg aaaaccatct caagctatgg aggctatgga aatgggagct gatgcagtac
    10021 ttgtcaacac tgctatagct tcagcaggag accctgtaca aatggctaga gcatttaaat
    10081 tagctgtaga aggtggaaga gaagcatata ttgcaaaaac aggaaatgtg tcagaatttg
    10141 caaatgcatc ttctccttta acaggttttt taggcaattt atagaaagtg gtgattggtg
    10201 atgagttttt atgatgtaat agaaaaatat agggattttg attttgatgg atatttaaat
    10261 aatgttactg ataatgatgt tttaagaagt ttatcaaaag acaagcttga agattttgat
    10321 atattaaatt tgctttctaa aactgcagta aaacatttag aagatatggc acaaaaagca
    10381 cataaattaa gtgttcaata ctttggtaaa acagtatgtt tatatacacc aatgtatata
    10441 gcaaattatt gtgttaatca atgtgtttat tgtagctata atataaagag tggaataaaa
    10501 agaaaaaaat taactatgga tgaaattaga gaagagggag aagtaatttc aaaagaaggt
    10561 ttcaaacatc tacttgtttt gactggagaa agtagttttc actcaagtgt agaatatata
    10621 ggtgaggcta tagaaatatt aagagagaaa tttccctcta taggtataga agtttatcca
    10681 atggaagtag aagaatataa gtacattgta gataaaggtg ttgaaggatt aacagtatat
    10741 caagaaactt atgacgagga aatatataag agagtacata taaaaggccc aaaatcaaat
    10801 tataaattta gactagatgc tcctgaaaga ggggcaaaag caggtatgag gactttatca
    10861 ataggtgcac ttttagggct aaatgatttt agaaaagaaa ctttttttac aatattgcat
    10921 gggaaatact taaaaacgaa atatcctcat attgagcttt catattcaac accaagaatg
    10981 agacctttta aagggtgttt tgaggaattg gtagatataa gtgatactga tttagttcaa
    11041 gctatggtat gtatgagatt atttgaccct catgcagcta taaatatatc cactagagaa
    11101 aatttagaaa tgagaagtca tataatacct ttaggagtaa ctaaattgag tgcaggtgta
    11161 tctactgatg taggaggtca ttctcaagat gaacatgata ctgctcaatt taagataaat
    11221 gatgaaagta ctgttaaaga tgtggagaaa atgctaaact caattggtta tcagcatgtg
    11281 tttaaagatt gggaaagatt ttaaatgtat ctgataacaa acagaaaatt atgtagtgag
    11341 gaacgatatt tagaagttat taaagagtct atattgagtg gtgtagaaaa tataataata
    11401 agagaaaagg atttagaata tcaagagcta agaaaattat atatgaaaat aaaaactaaa
    11461 atcaattgta tagattttca agagcaaata tcagatgaaa gtttgaaaac taatataaat
    11521 caaaaagagt ttagaaataa attcaaggtt aattttataa taaatagtaa cattgaattt
    11581 tttgaaaaag tggattgtca aggtatccat cttcctttta aattattctt gaatttaatt
    11641 gaaaataagt ataattttaa tgaaaataaa atactaggat tatctttaca taaagtagag
    11701 gaagtagatt atttagaaaa attaataaga aatcaaaata taaaaataga ttacataaca
    11761 ttatcacata tatatgaaac taaatgtaaa gaaggattaa atccaaaagg aattgagctt
    11821 ttaaaagaag ctaaaaagat tactgatatt aaaataattg cacttggggg aattttacca
    11881 tctaatgtca aggaaacatt aaaatactgt gatgattttg ctataatgtc aactataatg
    11941 aggtctaaag atattaaaaa aaccatatca aattataatg aaaagttgaa ttagtaaata
    12001 tagtatattg aatttttaac ttaaataaaa ataaaaaagt tatcttttaa attttaaaag
    12061 gtagcttttt tattttatat ctttgctaat aaaaaattgt aaaaagggaa aactttagtc
    12121 agagagatta aacaatatcc tagaaagatt taaaatttag taaagtatgg aggacattat
    12181 aatgactgac agtaaaaaga aacgatttat tataccacac acttttacaa taatattttt
    12241 actaatagtc ttgatggcaa tattgacttg gatagtacca agtggtgaat ttagtagaga
    12301 agatgtagaa ggtagaatgg tagttgtacc agggacttat caaagtgtag aaagtaatcc
    12361 tcaaggattt gcagatgttt ttaaagcacc aatagaaggc tttatcgatt cagctgaagt
    12421 tgtaggtttt gttttaatag ttggaggtgc ttttggtatt gtaaatagga ctggagctat
    12481 agaagcagga atagcttcgg ttatctctaa gtttaaggga aaagaacttc ttataattcc
    12541 aatatcaatg attttatttg gacttggagg tacaacattt gggatggcag aagagacatt
    12601 accattttat atgatatttg ttccactcat gacatccttg ggatatgatt ctctaacagc
    12661 aatagcaatt gtatttatag gagcagcagc aggagcagcc gcttcaacaa taaatccatt
    12721 ttcagttggt attgcacagg cactagctca acttgctcct ggttcaggag taacttacag
    12781 agtagttatt tttatagcat atatactaat aagtatagca tttgttatgt actatgctaa
    12841 gaaggtaaag gaaaatcctg aaagttcaat agtatatgaa atagataaaa ataataaatc
    12901 aatacttaat caaacttcaa cagaaataaa agaatttact tttaaagaag tatctgttct
    12961 tgctatattt ggaataggga tggcaataat gatttggggt gttttaagtc aaggatggta
    13021 cattccagaa atatcaatgt gttttgctgc aataggtgtg ctttctggat tggttggtag
    13081 attatctcaa attgaaatct cagaaagttt tatagatgga gcaaaagatt tagcatctgc
    13141 agctcttgct atagctttgg ctcgtggaat cgtaataatt gctcaaaatg gatttataat
    13201 agatactata ttaaattccg cagcagcctt tctaggtggg cttccaaagg ttatatttgt
    13261 atatttatca tttttcttgg aagctgcatt ggcattttta attccatcat cttcaggtct
    13321 tgcttcactt acagtacctg tacttgctcc tcttggtgat ttggttaata tatcggctca
    13381 agtgatagta accgcttatc aatttggtgt gggtcttaca aactttataa ctccaacttc
    13441 aggagtactg atgggagcac ttgcagtagc ccatattcca ttttcaaaat gggtaagatt
    13501 tataatgcct atcttgttga ttttgacagc tgtatctcta gtctttttaa caataggtat
    13561 ttatataggg ttttaattaa gtgtagtttt atttaagata tttagtaatg gtataaaatg
    13621 ataatatgtt ttcaataaat tgccactaat ttaattagtg gtaatttgtt ttttgtattt
    13681 aaaaataaat gtat
//
LOCUS       DAELTA010000101        13424 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.101, whole genome shotgun sequence.
ACCESSION   DAELTA010000101 DAELTA010000000
VERSION     DAELTA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.101"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(153..2564)
                     /locus_tag="KPG13_002500"
     CDS             complement(153..2564)
                     /locus_tag="KPG13_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0986210.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            2754..3155
                     /locus_tag="KPG13_002501"
     CDS             2754..3155
                     /locus_tag="KPG13_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG0986211.1"
                     /translation="MILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFLLSNSL
                     SWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLMIDLFS
                     LNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(3220..5337)
                     /locus_tag="KPG13_002502"
     CDS             complement(3220..5337)
                     /locus_tag="KPG13_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0986212.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(5368..6075)
                     /locus_tag="KPG13_002503"
     CDS             complement(5368..6075)
                     /locus_tag="KPG13_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0986213.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(6182..8053)
                     /locus_tag="KPG13_002504"
     CDS             complement(6182..8053)
                     /locus_tag="KPG13_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBG0986214.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(8397..10742)
                     /gene="secA"
                     /locus_tag="KPG13_002505"
     CDS             complement(8397..10742)
                     /gene="secA"
                     /locus_tag="KPG13_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG0986215.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(10978..13158)
                     /gene="slpA"
                     /locus_tag="KPG13_002506"
     CDS             complement(10978..13158)
                     /gene="slpA"
                     /locus_tag="KPG13_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG0986216.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
BASE COUNT         3929 a         2459 c         1455 g         5581 t
ORIGIN      
        1 tttaaatgat aaaaatataa aacaacaaaa aagagtgcct catgaactaa aaagttcatt
       61 ttgagacacc ccgttttgtt attttatatt tataaacttc tattgaatat tttttaataa
      121 taaaatgtat atccttacta tctttccatt cactattttc ctaaaagagt atttagttca
      181 ttaaaagcat tttcatttcc atttccacca actctaatag gagtttctat tttcttagac
      241 ttaagtacat ttttttgact ttcatctaac ttatttccaa ctaaaactac tggtgaatca
      301 gttttagcac ctaatgctcc tactgctagt ccatctatca agtctccttg actctttgaa
      361 ccatctttaa caacaaaagc atatttcaaa tctgagctag gatagaattt atctattatc
      421 ttagcattag tttcatttct attacttcct gcaagtcttg ttggatttga aagcttactt
      481 tctaaatttg atgataaagt agctgttcca ccaattatat aagacttagt tattttagat
      541 gaatttaaaa actcatcagc accttgcaac tcactctttt cattagtaag tattattggc
      601 atattgttat cagcagctgc tgcaccaaca cttattgcat ctgcaagtcc atttacacca
      661 tttgccactg caacttgtga aacagatttt atactattta acttttgagc tagagatata
      721 ctggtcttgt atctatcact acctgaaatt ctttctactg atatacccat attactaagt
      781 tgagtttgta cattcttgct taaagaattt tctccaccta ttagataaac ttttttagtt
      841 cctaatcttt gtaactctgc cttagttttt gaatctaagt tatcttttcc tgtcaaaaga
      901 attggagagt caattgctgc tgtaaatggt gtagctgtta aagcatccac tattgcatct
      961 ccatttacta atactgcatt ttgtgaagaa gtccatcctt tttgactaac tttaactgct
     1021 gtttcatatc tatctgctcc agttaaagat tctcctgaag tagcttgtcc agaagatgtt
     1081 tttgttatag cttttataac aaagttacaa ggagaaaatt cactatatgt atttatatct
     1141 tcaaatttat tgttttgaag tatgtagctt tcacctaagt ttgctttagc ctcatataga
     1201 tagtatccat ctatatcagt ttcatatgct aaagttgatt tttctctatt aggatttgct
     1261 gtgttgtcta taactactac tattgctcct ttaccttttg gtaagctgta agaattagta
     1321 ggtacattta tgtatccaga atatgatact gttccacttg caagttttgt cattgaattg
     1381 ttttgaggaa ctccatttac tactggtgca taatatactt catattttgc tcctacagaa
     1441 tctgtttcaa acataacaga gtcaagtttt tcagaatctc tgctaaaatc aaatacattt
     1501 gctgctgtta ctttatttga cattatcttg ctaagaccag catattcaag ttggtacatt
     1561 tttttatcgc tatctggttt tgatactgat ttcattgcgt agttatctgt atcttttaaa
     1621 agagttttgt cttcataaga aatccagaaa aagccttttt ctgagttaaa ttctccccaa
     1681 ctacttttta ctaaccatgc tccatttgat tctggtctaa cacttggttt gaacttatct
     1741 tttgaataat tatcatccca tcctacaatt gaaacagaat gatttaaagg ttcactttta
     1801 tcattacaat tatacgaatt ttcatcatca cttaagtagg ctgaatagtg tgcatatcct
     1861 gatgtcactg caccatattg cattattgca tttttaacag aagttttatc tttactaatt
     1921 cttacaacat ctgttacatt aaattgtgta ggtgcagtat ccatattttg aggcctagtt
     1981 gctccttcat cttctgattt aaaattatat ggaatgtctt tttccagctt aggtcctgcc
     2041 caggctgtaa gatatccaat tgcagttaca ttcgaactac ctgtcatatc agttaagttc
     2101 catccatgta ttcctccagt tgaccaccat ctaaaatgct cttctgaaag atcataagtt
     2161 ccataacctt ttaatttcaa atatgcttct agtgttgaca ttccagaaaa agcccaacat
     2221 gtattaagtt ttccttggtc ttttgctggt gtagttaaat ctaattttct tggatcatat
     2281 gatacacttg atttatatga tgctggaaga ctacttctta cattagtttt aagtgttgtt
     2341 ccttccattt cataaggagt tggtattact ccattatatt ttgctatatc tccatttttt
     2401 acatcttcca agtattgaag atatgattca gaatactctg cagtttctac tccagtcaga
     2461 attttatttt tttctgctga gacaggtatt gttgacacaa ttaaaaaaca aactgtcaac
     2521 aacaccagta gctttgacaa tttttttaat ttatactttc tcatatttac ccccttgtat
     2581 gtatttaatt tttattatat cagatttcta aatctaggtc atattttatt tttataaaaa
     2641 taggaattat aattatacat atataaaata ctattttata ctgcatattt attgattaat
     2701 atgtggtata ctaattacat aataatatat accaaataat ggaggaaaat tttttgatat
     2761 taaaaaaaca tctagaaaca atattatatc ttttttttgg tgctttaaca gttttaataa
     2821 acattattac ttacctattt tttacaagaa ttattaattt tactttttta ttatcaaact
     2881 ctctatcttg gtctatagca gttttatttt catatataac aaacaaattt tttgtattca
     2941 aaagtgaaaa aactgagttt ttatttctat taaaagaatt tgtatctttt ttatcttgtc
     3001 gtttattttc tggttttata gaaatattac ttatgtattt aatgattgat ttattttctt
     3061 taaatgatat cttagtaaaa ataggcacta atataattgt gataatttta aacttcgtct
     3121 tcagcaaggt agtagttttt aagcatagaa attaacatta ttatatttat ctttatgcag
     3181 aataaggagt tgcctaaaaa aaacaactcc ttattagctt taagaaacaa tttctatttt
     3241 atctgttgct ccccattttt caactttatt attttctcct gatgctaatt ctacataaag
     3301 tccatttacc ttatttcctt ctaaaattat atcactatta gaagtacttt tccaactgga
     3361 gtctatttca tctcctacat tcatagcttc tggttttgta gtctttattc tatagtaaac
     3421 tttattacca ttgtatccag ttacactaat agttacttta gaattttcat caacagaaac
     3481 agttgatttt attccatttg ctactatttt aaaaccatca tctgtcgcac cagctcttcc
     3541 ccatcttgaa actttctttc ttgtataatc aatttcaaca acttctatat atgatccatt
     3601 cttagcattt acttttaatg gttccgtatc ttcaaccaag tcccaatcat ttatgttaat
     3661 tctatcacca accctcatag ctgatggttt atatgacaaa actctataat aaattttata
     3721 atttgaattt ttaggtttct gaggtacagt taacataatt tgattattct caagaggaga
     3781 acatgcactt atctttggag ataatcctgc tgctgtatca ctatcatcat ctttatctgt
     3841 attattagtg ttaggtttat tgccacttat tcctggttct ttatttccac taacagtatc
     3901 aatctttccc gaatttctta tattagtact tgaagaatca ttatctatct ttgttatagt
     3961 tccatcattt cttatattta catcttttgc agaactaaat attgttacaa tccatatttg
     4021 tccattattt tcattttcaa tcttgcaacc attatcatca gaaatgtcca tttgatttat
     4081 atatcctgaa ttagttaaag ttccatcttt tatatcttta agtatcaatg ttttacttat
     4141 atcacctgta ttattcaact ctccatttgg catatttata gttattgact gactatattc
     4201 tccatataaa tcaaccttta tagctttttc agtctctaat ttaaaagatt cacttatttt
     4261 tttatcaggt ctaaacttta taatatcatt agcatccact ttctttaaag cagaatccaa
     4321 ttcttcaatt gtttcaactg tatatttagt ttcttcttgc atatccaata tatctttata
     4381 gacattttca tttcctattc caccaacctg agtaactgta ctaaatatct tagtatttag
     4441 tacatctttc tgaatagaat caattttatt tcctactaat accactggag aattgttttt
     4501 tgcagccaac actccaactg atagtgaatc tattaaatca ctttgttttt tcattccatc
     4561 cttggttata tatgcatttc ttaaatctgt ttctttataa aattctttta taacattagc
     4621 atttgtttca tttctattat ttcctgatat cctcttagaa tttggtaaat ttttttctat
     4681 ttcattagaa atggaattta atcctccaat aatataagaa ttttttatat tttgggcttt
     4741 tataaattta ttagctactt caactccaag gtttgaatct gaaagtataa ttggcatgta
     4801 attttgagct gctattgaac caacacttac agcatctgct aatccctttt ctccatttac
     4861 tacaattata tcagaaatat ctttttcatt attcaatttt tcagccaatt ttaaggatgt
     4921 ttcatatcta tcattcccaa aaattctttc aactgttatt ttctctgatt ttaattcatt
     4981 ttctacattt acacctagtg catttgaacc tcctactaaa aatacatttt ttactccaag
     5041 cctttttagt tctgattttg tcctaaaatc caattcttta cttccagata gtaaaatagg
     5101 tgcatctttc aatttagcaa atggagttgc agataatgca tctgctattg agttatcatt
     5161 cactaataca gcattatcag atcttaacca accgttttgg cttattttta ttgctgtttc
     5221 ccatctacct gaacctatta aactgtcttt attagatgca tttaccacat tactaggtaa
     5281 aacaattgat aaaaatgctg ctgttaataa agatattatt ttttttgata tttgcattaa
     5341 ttatctcctc ctaatttaaa tataatttta tggagcatgt acatactgtg ttttatttaa
     5401 ttctaaatca tcaaaatgag attttactga atggtatcct atagcataat gtccactttc
     5461 aggattccat ttcttatatg cattcatcgc acttaataac ttctcctctt gactaggtaa
     5521 taccttccag acttctacat ttggattaac ttttttaaaa tccttatagt catatggttc
     5581 tacataaaat cttaaatctt ttatttttcc ttctctatat aactctagtg cagcttctcc
     5641 taatgctcta tgatcttcat gtattccact agctttaaac ggagtaacag tttttacttt
     5701 tgcttctgga tattcttcta agtatttaag tattatttcc tttgcctttt ctttgcttaa
     5761 ctcaccatca tgagctctat tatcttctat atgtatatta ctttctttta cacccattgc
     5821 ttcacagcta tccttaaatt cttcatctct atcttttgaa aattcacttt ctgataaaga
     5881 gtatttatga acatctttaa tatgataaga acatgaccct ccatcatcca aaacttttct
     5941 tattatagat ttactaccat ctgtgcacaa tatgacatgt gtatcacttt tttcaacatg
     6001 ttcaattata gcacttccca tacttaaggt ttcatcatct tgatgtggtg taaaaaacaa
     6061 agttgcatct accactacta catcctccta ctttataaaa tgttataatt tacaataaat
     6121 agcataatta tagaaaaaga ttcctgtatt aattaaggaa tcttctccac agtataagtt
     6181 attaccaacc tagtatttta gctatctttt gtattactgt caattcaact ccattaccta
     6241 tttgagttac ttctgcacca agatttaatt ttttactaag tgcattcttt tgtgagtctg
     6301 ttatatcagt tttagctaga agtattggac ctttacctgc tgctagaggt cctgctgcca
     6361 atgcatctac tagcatacca ttatttccat atccatcttt tgctatatat agtttatcta
     6421 atttgccata atatgtttct ataactttag cattagtatc gtgtcttgtt tctccttcaa
     6481 ctctttgaac ttttctatct ttatcaatat ctgcaagttg attttcaatt tggctagata
     6541 cattattaga gccaccaact atgccaatct cttttccatc catcaatttg atagcatctg
     6601 ctgatagatc attccaacca tttactatta taggagcctt aagttcagca gctttagcag
     6661 ctatactcat agcatcagcc tccccttttg cgcctactac aaatgcatct ttaaaagacc
     6721 ccatagcttt tgctactgct acagaagtca tatgtctatc atctccagct agtctttcaa
     6781 cattcttagt tactgattct aattgctttt tagctgtatt tgatactgct gattctccac
     6841 ctactatata tattttagct gatgggcttt tcttaattat atcttttata taatctaaag
     6901 ttacttttgg tatttcatta tcagatgcta ataatatagg tgctttttta gattgagcaa
     6961 gtggtgtagc tgctaatccg tcaactagtt tatttgaatt aactataact atattgtcag
     7021 tatattttgt ttgttttgct atagccactg cagttgcata tctatcatct ccagctatca
     7081 atttaacctt tgaattacct tctaatgcag ctttggcatc ttttaaatct ttttcattgg
     7141 tactttctac agtaagctct gttgcttttc cagatgcatc agtatactta atagtaaatt
     7201 tatattttcc tgtttctgaa tcttttgctg aaatatcact aattgacttt gatgtaccat
     7261 ttgctaccat attaccttta acagtcaatc tatatccatc atataaatct tgtggtgaaa
     7321 ctgtatttag gtcactattt tttatagtta tttcggccaa ttggacatca tcaatatttt
     7381 tagctcctgc tacacctagt ttatcgattt tatttactct aaatccttct gcagaagggt
     7441 ctaaatttgt tccagttgaa tcaactggat ttgctgttaa atcaactttt tcatcaccaa
     7501 tacctacata tatagtttta gaagttactg tttctgtttt atctcttaaa gttataacta
     7561 acttatcttt agcactatca tatgaagctt ttacatctgc aactttataa attccattgg
     7621 tttcagtttt agctttttct gttatagaat tagcctcagc ggaaaggtct gctgaatttt
     7681 catatttttc agttgcttct gcaactactt catttgcact atttgttata tgtcctttat
     7741 ctgttatagt tacaataagt tctttatttt ctcccaaagc atttactaat ttctctaatt
     7801 catctatatt agttattatt tttaattttg ataaagtctc accaactttt atttcatata
     7861 ttggttgtcc aacttgagaa cctcctgtat acttttgaga cattaaacct ctaactttcg
     7921 aaactaattc tgtagcctct ttcttagtta ttgtttcttt ttttacctgt gtagtttctg
     7981 cagcaaaaac tggagctact gatgtactta tcattgcagc agccataatt acagaaagat
     8041 tttttttatt catggtattt tccccctaaa aaattcaaat ttttattatt ttttccttat
     8101 ttaccaatta taatatatat ttgatattat ttcaataaat gagacttatt gtaataatta
     8161 accaaaagat ataaactttt tcttatattt tattttttta atatttacgt atacaaaaaa
     8221 gtaacaaaat tgtaattttt tttaataatc cagtttataa ctaatagact tttcatcttc
     8281 tttatagtat aatattaaat ggaagaaaga aatttcaagc atctcttttt tccgtatttc
     8341 tcacatagaa aaagaggctg attatcagcc tctttttcct atacagttta aatttcttaa
     8401 ttaaatttat ataaatactg tatagtcgtc tctcttatat ttttatttaa tgattcaaac
     8461 atctcataac cttccatagc atattctttg aaaggatctt tttgagcata tgatttaaat
     8521 cctacatatt gttttaattg ctccatagca tctatatgtt ctacccaata ttgatcaact
     8581 acttttaaaa gaacaatttt ttccatttcg gttattttgt caaggcctac taccatcttt
     8641 tttaaatcat aaactctttt tgatatttca taagtactat caattatatc ctcaatactt
     8701 ttattatcta agtcaggtat tagtaaggta tcagctggca taaatgtatt ataaagatgc
     8761 ttaaaatatc catagtaatc cctctttctt tttgttaaat attcagcagc agcttctttt
     8821 attatatctt taaccatgtt ttgaatatct acttgtatat cttcatcatt tagaacttta
     8881 tttctttcgt tatagattac ttttctttgt tcatttatag tatcatcata tttaagaaca
     8941 tttttcctta attcaaaatt tttaccttcc accccttttt gagctctctc tattgccttt
     9001 gtaagagatt tactttcaat agaattattt ttatttgaat ttggtcgttt cattagtttc
     9061 tctatacttt ttccaccata caatttgatt acatcatctt caagactaac aaaaaacctt
     9121 gatgttcctg gatcaccttg acgtccagaa cgccctctaa gctgattatc gattcttcta
     9181 gattcatgtc tctcagttcc tattacataa agtcctccta attcctttac tctactttct
     9241 tcttctttat cacctgctcc taaagcaata tctgttcctc tacctgccat attagttgca
     9301 atagttactg aacccaactt accagccttt gacactatca atgcttcttt atcattttcc
     9361 ttagcattta gaacttgatg ttttattcct tctttcttaa gcataccaga taacatttct
     9421 gatttctcaa cagaaacagt accaataaga attggctgtt ttgttttatg tatccttatt
     9481 acttcttcta caacagcttg atacttttct ttctctgttc taaacacttt atcatgaaga
     9541 tctgctctta taattggttt gttagtagga atttgtacaa catttaattt ataaattgat
     9601 tcaaattctc cttcttcagt tttagcagtc cctgtcatac cagaaagttt attatataat
     9661 ctaaagaaat tctggaatgt aattgtagcc atagttttag attcatcttt tatttctacc
     9721 ccttcttttg cttctatagc ttggtgtaag ccatctgtat atcttcttcc atccattact
     9781 cttcctgtaa attcatcaac tatcattact tcttgattaa caacaacata atcaacatct
     9841 ttttccataa gtttgtgacc cctaagagcc tgatttatat gatgatataa ttctatattt
     9901 tctatatctg taagattttt aatcccaaaa aatgattctg ccttatcaat accactttcc
     9961 tttagagcta cggtcttatc ttttctatct aattcataat cttcttcttt tattgttttt
    10021 ataaatgaat ttgctaattc ataaagtttt gaatcttcat caccctctcc agcaattata
    10081 agtggagttc tagcttcatc tataagaatt gagtcaacct catcaactat ggcaaaattc
    10141 aactctcttt gtactttatc aaacttttta gttaccatat tatcttttaa ataatcaaat
    10201 ccaaattcat tatttgtacc ataagtaata tcacaattgt attgaacttt tctaacttca
    10261 ggtataagat ctgcaataat tactcctaca gacataccta aaaactcata cactggttgc
    10321 attagctctt tatcacgttg tgctagatag tcatttactg taacaacatg cacaccttta
    10381 cctgttaaag catttagata aacaggtgca acttctacca aagttttacc ttcaccagtt
    10441 ttcatttctg ctatttttcc ttgatgaatt acaatgcctc caattaattg aaccctatat
    10501 tgacgcattc ctaaaactct ttttgaagct tctcttgcca ctgcaaatgc ttctggtaat
    10561 aaactatcta aagtttctcc tttttttaat ctatctctaa ataggtctgt catttttttt
    10621 aattcgtcat cagacatatt ttctatatct ttttctaaag attctatttt atcaactatc
    10681 acattaagcc ttcttatttc ctgctcatct gctttatcca atattgtatc caaaactgac
    10741 atatttaatt tacctccttt tttatcatac aattactatt ataccttaaa attatagaat
    10801 agtagacaat tatttttatt tatagtataa taatcatact taaataaatt acatctttaa
    10861 ttatttaggt atttctcaca aaaaaaggaa ctatttttag ttcctttttt aatttctttt
    10921 tataaaaaag agaacttctt caaagaagtt ccctcttatt tatagtttaa ttttatctta
    10981 taattctaat aaatctttta ttctcttaat aactgaatct gctattcctt taccaacttg
    11041 agtcaatttc ttagaatcat cagaatttgt aactttactt attgctactg attggtcaga
    11101 agataaacta tcagttgcta aaactattgg agcttcattg tgacctgcta ctgctgctat
    11161 tgctaacgca tctactaatt gatcttcttt tgtagaacca tctttagcaa catagaagtt
    11221 tttaacacct gtgctaatta cactgtcact attatctttt tcaaaataag tttttataac
    11281 ttctgcatta gtatcttgtc tatcatctcc actaactctt tctggactct tacctgtagc
    11341 atcatctata gcatcttcca tgtcttcaga aacactagat tttccaccta ttatatctac
    11401 ataagcactt cctataaagt cttttgcatc agttgataaa tcttttcctt ttccatcaac
    11461 tactactatt ggcatttctt tattagaagc aactgatgct atactcattg catctgctaa
    11521 tccagtacca ccaactacaa atactttatt atggtttaaa cctatttcat cagcaatttc
    11581 taaagatgta gcatatctat catcaccaga taatctttca acttttaatc ccatatcttt
    11641 taattcattt gctacttctt tagatacaga attttcccca ccagctatat aaacagtctt
    11701 tttagatgtt ataccagttt tatcatctaa tcccattact ctctttattt cagattttac
    11761 tgatgaatct aacttatctt ttgaagttaa taataatgga gcatctttaa cagcagctaa
    11821 aggtgaagct actagaccat ctactattga ttgtgatcct actaatacta cctcattagc
    11881 ttctacataa tctcctttaa ttcctccatc tgcattataa gagtctttac ttatttctat
    11941 tgcagtctct attctatcat caccagctaa tacagaatta tttgaataag aattattata
    12001 agttcttaat tcatctaaag ctgattttaa gttattctta ttagtagatc ttaatgttat
    12061 tatagcagga gtatctactc tatcacttgt aggtaattct ggcttatagt tagatgatgc
    12121 atatgttgaa gctgtattta atcttttacc tgttggataa agaactacct cataataatc
    12181 attgtcagtt ttttcaaaat cacttgctgt tactgtctta tatgcatctt ctaattcagt
    12241 atctttaaat acatattttt tagctaaagc ttctgctttt tcttttgcat ctccagatat
    12301 tgtaactgtt tcatctgatg ctgctataac tgttacattt gattcaactg ctccaacttt
    12361 ttctccagtt ttttcaaaac ttaattttga tgtaccatca gtagtattaa ccgttataac
    12421 tggtttacta aaatcatata tctctttagt accatcaaga gatgctactg ctgtatctaa
    12481 agtaggtgct actgctgctt gtgtatcata ttttaattta attgcaccag ctactgcatc
    12541 agcatatgtt gctgtaatag ctactgttcc tgtagcatta ttaccagtct tatcagtaaa
    12601 ttttgaagtt ttgactgctg cctttgtagc tgctaaaact tcatctttag tagctccatc
    12661 tacataagct gacgtagttg tatcatattt ttgtaacttt aatttagcat cattttctat
    12721 tgaatttcca tcttgatcta caatcttaac tgttgttgca gtaccaactt tagtaactac
    12781 agcattacta tcaagttcct ttaataatgt atcatcatat tttttttctg ttccatctgt
    12841 aatttttaca gatatttttg catattctcc agcttttaaa tctttaattt catctttaac
    12901 ataacttatt tgacttgctt ctaaacttga tatagatacc ccttttggat ttcctttttt
    12961 gtcaaaaaag ttaactgtta tgcctgttac accatctgca acatattttt taagtattct
    13021 ttctatatca ctatacttac tactagatac agtggtattt ccatttggaa aactatcctc
    13081 tgctgcagcg aaaactggtg ctgctgatcc gattactgta acggcagaca ttacaactgc
    13141 caaatttttc ttattcattc cttaaattcc tcccaacatt atattaatta catctcttta
    13201 attatgtaca ttaaaattcc atgttcatat aaaccaaata tttctcacac tcttatgata
    13261 gactatataa aatagaattt caataattta tgcaaaatta atagaatttt ctttcaacat
    13321 ttttcccctt ttttttgcta tttcatattt tttaaattaa attttcaatt ttaattttat
    13381 taatacattt atttatctga tttaatcaat atatattaat attt
//
LOCUS       DAELTA010000102        13324 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.102, whole genome shotgun sequence.
ACCESSION   DAELTA010000102 DAELTA010000000
VERSION     DAELTA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.102"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            191..1528
                     /locus_tag="KPG13_002507"
     CDS             191..1528
                     /locus_tag="KPG13_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG0986217.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1864..2433)
                     /locus_tag="KPG13_002508"
     CDS             complement(1864..2433)
                     /locus_tag="KPG13_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0986218.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2803..3408
                     /locus_tag="KPG13_002509"
     CDS             2803..3408
                     /locus_tag="KPG13_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBG0986219.1"
                     /translation="MTNWNLWRTLNMEYIINPCSGIKIKITRGQKIEIVDIEGGQVVD
                     FFAEMKDDSTEFISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLS
                     KANDKIILEALENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(4087..4989)
                     /locus_tag="KPG13_002510"
     CDS             complement(4087..4989)
                     /locus_tag="KPG13_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG0986220.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
     gene            complement(5116..5235)
                     /locus_tag="KPG13_002511"
                     /pseudo
     CDS             complement(5116..5235)
                     /locus_tag="KPG13_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5781..6443)
                     /locus_tag="KPG13_002512"
                     /pseudo
     CDS             complement(5781..6443)
                     /locus_tag="KPG13_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(6603..7139)
                     /locus_tag="KPG13_002513"
     CDS             complement(6603..7139)
                     /locus_tag="KPG13_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG0986221.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7212..7625)
                     /locus_tag="KPG13_002514"
     CDS             complement(7212..7625)
                     /locus_tag="KPG13_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0986222.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            8091..8645
                     /locus_tag="KPG13_002515"
     CDS             8091..8645
                     /locus_tag="KPG13_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG0986223.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9253..9432)
                     /locus_tag="KPG13_002516"
     CDS             complement(9253..9432)
                     /locus_tag="KPG13_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0986224.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(9658..10191)
                     /locus_tag="KPG13_002517"
     CDS             complement(9658..10191)
                     /locus_tag="KPG13_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG0986225.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(11939..12511)
                     /locus_tag="KPG13_002518"
     CDS             complement(11939..12511)
                     /locus_tag="KPG13_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0986226.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12573..12836)
                     /locus_tag="KPG13_002519"
     CDS             complement(12573..12836)
                     /locus_tag="KPG13_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0986227.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12836..13057)
                     /locus_tag="KPG13_002520"
     CDS             complement(12836..13057)
                     /locus_tag="KPG13_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0986228.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         4670 a         2247 c         1562 g         4845 t
ORIGIN      
        1 aaatttaatt ttttgaaact taatttttaa aatttcaatc ttttaaaact caattttttg
       61 aagcttaatt tctagaattt tatttttcaa aatcaattct ttgaaatcta atttccagaa
      121 ttttaatttt taagatttaa ttttttaaaa cttagtttcc agaatcttaa ttaatagaaa
      181 ggtatattaa atgggcactt attctttaat ctctacaagc ataaatttta ttctaatatt
      241 aatcaatatt ttcatagcct tttttcttta taagttgact tcctatttta tagactttag
      301 gaatcattgg attttcaaaa tcatattgat tttgggctac tctattatta gtcaaatgat
      361 aatatggctt tccgacccca taaatatttt gtttacacta ttcggatatt ttatactact
      421 tataatatgt agcaaaagca aattaattcc tataatatca gttgtcatga tactttatcc
      481 tattataatt gggcttaatt ttctttttat aaacaaccct atatatgagg aacttataca
      541 ctcaaaagag tatattttaa tactttcaat agtccagata atttctgctc taattaaatt
      601 attattttgg tttttaatac atattttcct aaagaataga ttggtcaata taaaacaata
      661 cttatcaaat aaaatatgga tttttattga tatggtatgt atatcttcat ttttatcaat
      721 attaattgct attattctgc cacctactaa tttaatccct atgggtagta ataagaatat
      781 tatcttaggc aacttacctt atggcactta tctaatcatc ttgtcagcaa ttctggcaaa
      841 tatcggtgtg atacttcttt tacagccatt gattgaaaat gtaaagatga agatagaaaa
      901 acaagcaaat cacctaaaag aaaaatacta taacttacta gaaaatcaac aaattcaaat
      961 acgcaaaatg cgacatgata tgaataatca ctttcaaatg attgaatcgt atttagaaat
     1021 tgaagatatt gctggagcta aaaagtattt taatcagtta aaaataggta taggtactct
     1081 tagtggaaaa caattttgca aaaaccaagc tcttaactca attttaaatc ttcgtcatac
     1141 aagtttagaa gaaaatggtg ttgatgtgca ctttaatata gatatagaca atcacttggg
     1201 tatagaacct atagatttat gtactatttt ttcaaattct ttagataacg ctatagaagc
     1261 atctttgaaa attcctgatt tgtcaaatag aaaagtggta ttaaaggcac gttgtgagaa
     1321 ggagtatttt agctatttat tgacaaacaa caaggtaaat cctatcaata gaaaacaaga
     1381 actatttatt tcagataaaa aagatagtaa ttctcacgga tatggtattg agaatattag
     1441 agaaattgtt aataagtaca atggaaaatt ggatatttct tatactgaga gtgaattttc
     1501 tttatttctt tatattagat tattttagtc taaaattaga ttatcttata taatacaaat
     1561 tagattgttt gatactatgt aaaattataa taacttataa ttaaatattt acacatatat
     1621 tcattaactt aaaatttaat caaatgtgat attttattta ccttagtaaa aatgtgtgtg
     1681 tctcaaaata aactttttag ttgatgaggc actctttttt acatgaaatc aaatatattt
     1741 ttatcctttc aacaatgaaa aatggattta tctctccatt ttgaaacaag ccctttttct
     1801 attaattact ttcttaaaaa atataatacg atatataaga taaatatcta attgtaccta
     1861 tttctaatta tatctattag aatacaacac ctttgccttt tcagtaaact ccctaacaaa
     1921 atctagttta ctatctgtat aagcatcacg attatgctca tacttcttcc aaaggtccaa
     1981 cttaagtttt tcatactcct ttgcaacatc tgggtggtca attaagtaat ctctaaaaaa
     2041 caactcatca ttatcaccta cataccttat atgcaaatga aaaactcgtt cttcaaaccc
     2101 ctctactgta taccctttat taagtgaaag cctactactg ctctcagaca tacaaatata
     2161 tccattcgac tcaatcaaat ctttaatatc tttcatatta taatcaggaa ttatctcaat
     2221 cagtatgtca acaatgggct tggcataaat cgtactcaca gaagtacttc caatatgact
     2281 tattctaaca attttttgca ttggcaatat tccagttagt ctatattgtt cttctttaaa
     2341 ccaatttttc caacattctt tgtgagtagt caaaaatata ggaaacaagt tccataactc
     2401 ctctaaagtc atatcttcca acttcttttt cattagttac ctccttttaa atttgtactt
     2461 tatatttttt ctttttcaaa tttacttagt taatatttaa ctttattttt tatcaaatca
     2521 tttttctaat tatgtaaatt tttctaaatt tagtttttaa tgtattataa cagattttta
     2581 cccttcttac acttttgtat ctatttaaat attagaacat tttttcttac ttctaaatac
     2641 agtaagattg cttaaatacc aaataggagc aaaaaaactc tgtaatctaa aaaaaactac
     2701 aacatcttta tgagaattaa aaaatgatag actatgttat caacattaag aaacagatag
     2761 aaaagaaaca gagtatgtgc taaaatggga acatagaaca gaatgacaaa ttggaattta
     2821 tggaggacat taaatatgga gtatataatc aatccttgta gtggtataaa aattaaaata
     2881 actagagggc agaaaattga aattgtggat attgaaggag gacaggtcgt tgattttttt
     2941 gcagaaatga aagatgattc aactgaattt atatctccag gtgttacaat tgattgtaat
     3001 gagtcattaa gattacaagt tgggaattta atatattcta ataagtatag acctatgttt
     3061 aaaattttat atgatgatgt aagggaacat gacttgctac atccatgttg tagacctgag
     3121 atgtacgatt tcttttatca caatggagaa aatcatccaa actgttttga taatattaat
     3181 cgcacacttg gaacttccca tcaaattatt caccccatta atttgtttat gtacacaaaa
     3241 gtcaatacag atgggagtat tgaagtatgt gaacctttat caaaagccaa tgacaaaatt
     3301 attcttgaag cattggaaaa catgacttta gggattgctg catgcagtgt ttcagaaagt
     3361 tcttgtaata gcagaaaatg tacttctatt aagattttgg ttgattgaca aatttcattt
     3421 atcgaaaaaa tatcaaaatt aaaaaatgac gtcaaaggcg caaaagggta accacttgcg
     3481 ctctcaattc attatggttt gttacgtatt tatgggtaaa aatcccttga ttctatgatg
     3541 tttttcagac acaattacaa atgagtaagg attttatact catactctaa aaaatagcat
     3601 tttattgcgt aacagaacga aaaaacagtg tgtacctttt tcttgcaata cacactgtat
     3661 tcatagccac tctagctgtt aactgttaat tatctcataa tacccttact ggtattcatc
     3721 taatcttaac cccttttaat tattttcatt tataaataaa ttctattctt cccaaaatat
     3781 cgtaattaaa atatattaat taaaatatcc caataagaat atctgaaaaa tcttctacct
     3841 attttttcaa accttttatt cccattcctc atacaatagt gcaactttta gcacagtata
     3901 aatataccat tagtccatat aatctcatta acaaatatat acaattattt tcttcaaact
     3961 cttttatatc taagatatca cattttcatt ttcttatatt aattccttaa ctctctttca
     4021 aattaccaat tttttaatca aatcttcatt aaatattcta tcattattaa acattcatca
     4081 ttatccctaa aaaatatgtg ccgtatgttc tttacacaaa ttttccatct tatcatacac
     4141 agcaccacta tctctctcat ctgtcatagt cacaattaca tcacttggtc taagaattgt
     4201 cttacctcta ggaataattt cttctccacc tctttgaatt gctaccaaaa gacaattttc
     4261 aggccactgt acatcctgaa ttgccttgtt cgcaacagga gagccaaaca taacagagaa
     4321 ctggtctagt atcttttcgc ccctctcttt atgtacaggt tctccattct tttcaagtaa
     4381 cctctcaaga agactttcat aaataggttt agacttcata agtgtagcca caatatatgc
     4441 cacaattgaa attactgata gtgaaagcat ttgactaaca gaacctgtca tttcaaaaat
     4501 taatatgata ccagtcaaag gggcacgcac aatagctgta aaatatcctg ccatagccaa
     4561 aagcacaaag ttatttacat aatcgggatt cattccaaaa actttaacac ctaccatacc
     4621 aaatattcca cctataaatg ctcctaaaac aagcaatgga aaaaatattc cccctggtgc
     4681 acctgaccca aaactaacag ccgaaaatat aaaacgtata acaaaaatca aaacaaccat
     4741 tccaaaaata tatttatgac ttgtaagttc ttcaactaaa ttgtgtccac tccccagaac
     4801 atcaggcata acaagcccaa gaataccagc cattataaat ggaatcaaca gttttccaaa
     4861 cgtaccaatt ttaggaatct tcttgtataa agactgcaca tatagagtaa accagttgta
     4921 aaatgccccc ataactccaa gaataacacc taaaatcact attaatccat aataatcttg
     4981 tggcaacaca tttccaatct gaacctccca tgactaaagt cacagggttc ctgcttcata
     5041 gaattttgca tactaaaata tgtcttacta attctccaca ggctatcccc gtagttccta
     5101 cggttcttac ataagttatt tattttgaat tgttattagt cttaatcctt cttttaatat
     5161 atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac aaatccattc
     5221 tcttataata atgttaacaa atgtaattat ttaaaacaat tatttagaag gttttatatt
     5281 taattttaaa taacatattt aagaaccgta agtcttaact tttatcattt ttgaggttgt
     5341 cgttcacata agttcgctac tacctatgca gttctcttat gaacttctta cactttcatg
     5401 caagcacaga ctatatctta tccttcggca ttatccgtta aggtctaccc acttccacta
     5461 ccaatagctt gtagtgtact tccctcaaga ggaatagtcg ttgaagtttc tcctattcgg
     5521 agtttacctg ctgattgccc attttaaagt acttagggtt taaccttata ccatctaacc
     5581 aattttttct actttcgtaa ctttcacact taggtttatt tcatccttat gttttagttt
     5641 ggttagcttt aggggtttcc agcaattcaa gtagtattgg ataggttcta tcctatctct
     5701 acatataggt ttccctatat gctgactatt ttagctacta actcatgact aaagtcacaa
     5761 gtgttcgtag ctatttttta atcaaactgg aatacagact ccattcctag gaaattggct
     5821 gaaatatagt ctgctgtcaa agatgctgtc atcacagaaa ttaaaacaga tactgaaaag
     5881 tttttatgga cttcttcaag tgaaaacata acacccgcta gtggtgcatg aaaggctgct
     5941 gataatccag cacttgcccc acaggttaac aggaattttt cctctgtctt ccctcggtca
     6001 agagctctag aaataccttt acctgccata gcaccaagct gaatggaagg accttccctt
     6061 cccaaagcaa gaccacccat gagtgataag aatcctccaa taaattttgt tggcaacact
     6121 cgccaccata cttgttttat tttccctgtc atttccccct caagctgagg gataccactt
     6181 ccagaaatca ttggctccca gtccaccaac tttgatacaa tccacgcaag tactgcaagt
     6241 actgcaaacc aagctattat ttttattgta gaaccttcta caaattttaa aatttggcta
     6301 agccaagtac cagcctgccc taaaagtatc ctgtataaaa gcacaatcaa accagccaca
     6361 gccccaacca taagtccttc accaatcaaa attacctgaa agtgctctgc ccgtttgata
     6421 atatgagatg tatccttttt catactattc ctccttcatc caataattct acacaatctg
     6481 aatctccaat gactaaagtc atgagtgtgt acagatattt tttaatcaac aaaaatagat
     6541 ttttacttac taaatataag aaaataattg ctcaatttct caataaatgt gaatttattc
     6601 ttttaggtat ttattctcca cagtcgtgct tcttccagca aaatatttat taattcttgc
     6661 tttctctgtg tatagatttt acgattgtcc tcaaactgta atgctaattt ttgtttatga
     6721 ctatcataga gttttgcttt gtctgtaaaa gtatttagat aatctcgaaa attgatataa
     6781 ttattccatg caacactatt ccattcaaca acatgaatat ggtgagtacg agtatttttc
     6841 tcaaaatctc ccatagcaaa aagtaattgt tttggtacat cctgacctcg aaaaacgatt
     6901 ccacattgct ctaacctttc gaggtataat ttagcttcct ctaaagaagc aacacctaca
     6961 acaatatcaa taataggctt tgcgctaata ctgggaatag aagtgcttcc aatgtgttga
     7021 atatcaatag cagtcttatc ccatatactt tttaataata taatgacatc ttttgcactt
     7081 ttatcccata tcatctgatg ttccaataat ttaaccatac cacattttaa acctatcact
     7141 atataccacc tcaatataat ccacctttat atatattaca attttttcta tcttgataaa
     7201 tagtgattta gctatttcac tttttcttga cactcgctac attccctatc atgaatagat
     7261 ataattcctc cacatttaga acaagtatat ctctccttct gcttttgcat aaacttctct
     7321 aaaccgtact gctgaacaaa tttactattt tccataaggc ttgcttgata tctcttgtta
     7381 taactttttt caagattttt tatgagttta caaggaaaat tgaaacattc aaaacaataa
     7441 aacaaccctt tttgacagac acaatccttt attttgcact tacggcagtg ttctggcttt
     7501 cctgtatcgc cgttcaaaca accagcacat ggctttttgt gataacaatg cttatagcaa
     7561 accatacaat tcattccaca aggagcaaac ataatcgtat caatactctc ttttggcatt
     7621 ttcataacat tttactcctt tcaaaaatcc aatttgtcga attgttatat attcatattg
     7681 taacataaaa aatagctaaa aaattcatgc actttcattg ccaataaatt ttcaaggaaa
     7741 tatgaaagtt taaaactaag aataaaaaga gataaaaagg aagctactaa tcaaaatatt
     7801 caaacacaag taatcaatat aaataactta aggtatattt aaaagtatat gatattacca
     7861 gcagttttaa atacaaatat aaccacacat gcctcaaaaa cacttattac agtatcttag
     7921 aaacttagac aatacataag attcattaat aaaaaagttc tcaatataga tatatttaac
     7981 catgttttga atagtagata atcatatgat taaaaattaa atatgacata gcgtttgtgg
     8041 aatataacac ctaaattatg ttataataat aaagcaggag tgtgatagtg atggatgaaa
     8101 aaatattaga gttattacag gaaatgcaag gtaatattag taatatgcaa ggtagtatca
     8161 gtggtatcga aggcactatt agtgatatgc aaagcaatat taatggtatc gaagacacta
     8221 ttagtggcat gcaaggcact attagcgata tgcaaggcag tatcagtagt atgaatggca
     8281 ctattagcga tatgcaaggc agtatcagta gtatggatgg cactattaat agtatgcaag
     8341 acagtattac tgatatgcaa gttgaaatta aagaagtcaa tagtaggtta gacaatatgg
     8401 aacctgatat taaagactta aaaactggac aagttgaaac tagtagtaga cttgatacta
     8461 ttcagagtga tgttacagaa attaagtcag atgtaaaaat taacagaaat acattagata
     8521 cagtgattaa acatacctct tcattagtaa aagatacatc agatattaaa aatgatttaa
     8581 ataatataga gatagtcaca tctaagaatt cgaatgatat tgctaaatta aaatcagtta
     8641 aataatatac tatagtaaaa aatacctgaa aatttcacat acttctttaa agttatacca
     8701 aattttatac agaaatactg attatccgac aacacacttt acttataaat aatttattaa
     8761 aaaaatactc attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa
     8821 aaataaggga gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt
     8881 atataatttt tcagcctaaa ataaaagtcc atctttgatt aaaacacatc cccttatcac
     8941 ttataaaagg ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa
     9001 taattttttc ttttgatact ttaagtgatt ggttaaattt aaccaaacga ctaagttttg
     9061 ttcaacatct atttttcata ttttccaata cttaatctat tttttataaa taattctttt
     9121 atttctaact cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt
     9181 gtatggttct taatacttaa tgagtagctt aagtatcaat cattataaat ttttccctta
     9241 catttaacac tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg
     9301 atgaaataca atacatgaaa atacaatatc ataactgttg tctaaaaata gaagttcttc
     9361 aacataacct ttcacaaaaa acatattatc taagtatttt ttgtctgctc cttgttttcc
     9421 tgtttgtagc atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa
     9481 taaacttcca aaatacaagc tctaaaaact acctctaaag ttgtataatg tttactagga
     9541 ataaggttgt ataacatata gtttaaataa tcatttttta aaattgcaag ttattgcttt
     9601 caaaattaga tgtttttata tcaaataaat gattaagaat ttctaaattt aatggcttta
     9661 taatgcactc cctttctttg ggataaaata atgttgcaca gatacttttt ctaaagataa
     9721 caattttatt tttctatcaa ttccaccagc atacccagtc aaactcccat ttgttcctac
     9781 aacacgatga catggaataa taatagagat tccattatgc cctactgcac caccgattgc
     9841 ttgtgctgac attttagata ttcctttttg ttgagcaact ttatatgcaa tttctccata
     9901 agtgattgtt ttaccatatg gtatttgttg taaaattttc cacactgtag tctgaaaagg
     9961 agtacctacc atatggagtg atggtgtaaa attgggttct tttcccgaaa aataaatatc
    10021 taaccatttc tttgtctgct cgaaaattgg caattctttt tcttgatgtt cttggtcaag
    10081 attataagca taatactttt gattctcaaa ccaaagacca gttaacccaa tattatctga
    10141 tgctaaaaga atatttccaa ttggactatg gtaatagcta atatattgca tacaattcct
    10201 ccttatattc tgaaaacttc actcaaatca ttagacactt cttttctatc taccattatg
    10261 taaaaacata ctaatataat ttccttctaa ctacactttc gttataatat ctatcctcat
    10321 atcatattag ttttatattt acctgcatct gttcatcctt ttttcccttt ccctttgatg
    10381 aatataaaat tcatacattt aagcttccta ctgtattaac ttttatagaa gtgtatatta
    10441 taattttatc actaaatagg ccaaaaaaat atcgatatag catcaagtaa tatcataata
    10501 ctttcaaatg ggaaaagtaa gaataggctg taaaaaagaa aagtattttt atgttagaaa
    10561 tccagtaaaa tcattagtag atagaataac agaaaacgaa caatttgcta tttatcattt
    10621 tacacataaa tataattgac gcaataaagt gttttctatt ctacaacgat taataaggtt
    10681 agaaaaaagt aaaaatgaac tgtatatgta tgaagcatta gtttctgttt ctttcctttg
    10741 gcttattatg ataaaaaata ttgttttgct accaataaaa cacaaaaata taattaatat
    10801 ccatatgcaa aaaattttac gttatattga aaaatactat tgttttacat tatattgaag
    10861 ggtactattg tgaaaactta attttggagt actatcaagc agtggtaata tcaataaatc
    10921 agagtgctcc agatgatttg aactaagttt aaatactata ccttataaat attttactga
    10981 gttttgttta ttaaaagcaa ctcaatttct aaaaggcaca aatgagctaa taagtgacat
    11041 tgctataaaa gttgtttttc atcaaataat ctattttagt aaatgtttta aagaaaaaac
    11101 gggatattct ccaaaagtgt acagaaatat aaaaaaagag atatgatttt ttatttcttt
    11161 tgattatcca aagagtaaat aaaaaatatt gtaaaaaact tactaacaac ggcaatgctt
    11221 tgaactatgg taatactttg aacttttgat tggacttttt ttgctaaaat aatatattta
    11281 aatagcaaag taatctgttt aatcaataaa aaataagaga taaaagaaat caactaaaac
    11341 ttttacctct tattttgata gtttatcaga gctaatattt acactctttt tatttttgat
    11401 acttcattaa aaatgatgct tactctccat tcacttttaa ttcttcatta aaaatgatgc
    11461 ttattctcca ttcactttta attcttcatt aaaaatgatg cttactctcc attcgctttt
    11521 aattcttcat taaaaatgat gcttattctc cattcacttt taattcttca ttaaaaatga
    11581 tgcttactct ccattcgctt ttaattcttc attagaacta tgcttactct ccattcactt
    11641 ttaattcttc attaaaaatg atgcttactc tctgttcact tttaattctt cattaaaaat
    11701 gatgcttatt ctccattcac ttttaattct tcattaaaaa tgatgcttat tctccattca
    11761 cttttgattc ttcattaaaa atgatgctta ttctccattc acttttaatt cttcattaaa
    11821 aatgatgctt attctccatt cacttttgat tcttcattaa aaatgatgct tattctctgt
    11881 tcacttttaa ttctttatta aaactgatgc ttattctcca ttcactttca attcttcatt
    11941 aaaactgatg cttgcactct gtaaactcat aaacatccat taatgcttca ccaaatcttt
    12001 gatagtgaac tacttccctc tcacccaaaa atctcaatat atctttcaaa tcagggtcat
    12061 cagttaaatt tattaattga tagtacgtag ccaatgcttt ttgttcagca gccatatcct
    12121 catgtaaatc agttaccgaa tcgccaaaga cattaatata acttgtagta aagtttactc
    12181 cattagaatc tactggctga agaccatgtc caaaattagc ataattacta ccaagtccag
    12241 cagccttaag ctcttctgga gtacaattgc ctattaattg atataacact gaagaaatta
    12301 tctcaacatg agccatttcc tctgtaccta tatcagttaa aagtgcacga gattttccag
    12361 ttggcatagt atatctttgt tgaagatatc tagttgcagc tgccaactca ccattaggtc
    12421 ctcccaactg agtcatgata tattttgcca ttctagggtc tgcttgtctt atgttaactg
    12481 gatgttccag tgttttttga taaatccaca tattatctta ctcctcctta atatatattt
    12541 aataataaat ctaattttaa agtttaataa ttttatttat aaaatgattt gtcccatggc
    12601 caaggttcag atatccatga ccaaggatat tcacttgatt catatccaaa attagttaat
    12661 gctccatatt tagattcata atcacacaca gcttgtgtaa atttatcgca gtatctgttg
    12721 tatgaatcaa gagctttctt atcccttgga ttgttatcta aatataaatt taattctata
    12781 caggcaaatt gatattctga aattctctct aaaagttctc ttctatttaa tgacattagt
    12841 acatgttacc ccctttttta gctctaactc tatttttagg atttgcatat agccaatcat
    12901 tatagttatt tgactctatt aaatctaatt caggaaataa agtacctctt tttaatgctg
    12961 tttttggatt gtatgtgtca tcataaattt gacgattgat gtatggtcta gcaaattcac
    13021 aacaatagtc ttgtttatcc tcttttaaat atcccattaa ttatcctcct taatttttag
    13081 aattttaaaa tagcattatt tttaaaaatt gtatactggt tctatattat aactggtagt
    13141 tttaataggt ctttaataag attaaatgat taaataagta tctttttttc ttattttacc
    13201 tctaaaattt ttctaattaa tctttcataa atatctattt taaataaata atatagagtt
    13261 tatacaattt tttatagctt aattactatt tactatatat tattcatttt taaataaaat
    13321 gtga
//
LOCUS       DAELTA010000103        12490 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.103, whole genome shotgun sequence.
ACCESSION   DAELTA010000103 DAELTA010000000
VERSION     DAELTA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.103"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            57..1685
                     /gene="aspD"
                     /locus_tag="KPG13_002521"
     CDS             57..1685
                     /gene="aspD"
                     /locus_tag="KPG13_002521"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG0986229.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1806..2801
                     /locus_tag="KPG13_002522"
     CDS             1806..2801
                     /locus_tag="KPG13_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG0986230.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2979..3362)
                     /locus_tag="KPG13_002523"
                     /pseudo
     CDS             complement(2979..3362)
                     /locus_tag="KPG13_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3589..3686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3827..4385
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3827..3856
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4671..4829)
                     /locus_tag="KPG13_002524"
     CDS             complement(4671..4829)
                     /locus_tag="KPG13_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986231.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5123..6493)
                     /locus_tag="KPG13_002525"
     CDS             complement(5123..6493)
                     /locus_tag="KPG13_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG0986232.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6709..7826
                     /locus_tag="KPG13_002526"
                     /pseudo
     CDS             6709..7826
                     /locus_tag="KPG13_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8189..8269)
                     /locus_tag="KPG13_002527"
                     /pseudo
     CDS             complement(8189..8269)
                     /locus_tag="KPG13_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8309..8977)
                     /locus_tag="KPG13_002528"
     CDS             complement(8309..8977)
                     /locus_tag="KPG13_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0986233.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9830..12250)
                     /locus_tag="KPG13_002529"
     CDS             complement(9830..12250)
                     /locus_tag="KPG13_002529"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG0986234.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
BASE COUNT         4303 a         2018 c         1622 g         4547 t
ORIGIN      
        1 aacttgttta ttttattata aaaatgtgaa acttattaca taaggaggaa tttaacatga
       61 aaaatcatac ttataaagaa atcaaaaaca tatatggaaa aataagccct tttgaattta
      121 aggacaaatt aatagatatt gcaaaataca ctgcaaaaga aaataacaga gaattacttg
      181 atgcaggtcg tggaaatcca aactggactt gttcaactgc tagagaagcc ttttttactt
      241 ttggtcattt cgctattact gaaactcgtt ctaattggga tttaggtcat ttggctggaa
      301 tgcctcaaaa aaagggtatt aaagaaagat tttttaaatt tataaatgaa aatatagata
      361 tgcctggagc atatttagct agagatatta taaactttgg tataaatgaa cttggatttg
      421 atggagatga atttgttcat gaactagctg atggaattat tggtgataac tatccacttc
      481 cagatagaat gttacctcat atggaaaaaa tagtacacga ttacctagtt caagaaatga
      541 aatatgatat atcaggtaaa tatggtgatg tagaaatttt tgctgttgaa ggtgctacag
      601 ctgctatgtg ctatatattt gattcattaa tggcaaatga acttctaaaa aaaggagata
      661 ccatagcctt aatgactcca atatttacac cttatcttga aatacctaac cttcctcgtt
      721 acgactttaa agtggtaaat ataaatgcta atgaagttga tgaaaaaggt gctcatacat
      781 ggcaatatac aaaaaaagaa ttagagaaac ttcgtgacaa atctattaaa gctttattcg
      841 ttgttaatcc taataatcct gcttctatag ctatggatga aacatcttgt aataatctaa
      901 tagatgtaat tgaaaattat aataaagatt taatgataat atcagatgat gtatatggaa
      961 cttttgtaga agatttttca tccttaatgt caaaactacc ttacaacact gttggagtat
     1021 attcttattc aaaatatttt ggagttacag gatggagact tggtacattt gcccttcata
     1081 agaaaaatgt ttttgataag aaaataaatg atttaactgg agaattaaaa aaatcagtag
     1141 ataagcgtta cagtgatatg agtcttaacc cttctagcct ttcatttatg gaaagagttg
     1201 ttgctgatag cagacttgtt gctctcaacc atactgctgg tctttctact ccacagcaag
     1261 tacaaatggc gtttttctct gcttttgcat tgattgacaa agtagatgcc tacaaaaaac
     1321 tcaatatgag tatctgccat agacgtcaaa aactattatt tgaagcttta gagcttccaa
     1381 ttaatgaaaa caaaaacaat gctgcatatt acactcaatt tgatattgaa gaatgggcta
     1441 aattaaacta cggagaagac ttatttaaat ttataagtaa aactgcatct ccagtagatg
     1501 ttctttataa attagctaat gactattcag tagttttact tagtggaaat ggattctatg
     1561 gtcctgaatg gtcaattaga atatctctag caaatctata tgatgaagct tatactaaga
     1621 taggaaaagc cataagagag atattaaatg gttatatcgt tgaatggcaa aaaattaaga
     1681 aataattata ttacactgaa atatctaaat tatatgaata aaagagttta tgtagacctt
     1741 aactactttt tttgatataa tcctatcgga attataaatt ttatttatga aaggcagaga
     1801 atagtatgag tattaagaaa aaagttgcca tagtttttac tggtggaact atatctatga
     1861 cagtagatga taaagtcgga gcagctatac caactttatc aggtgaacaa atactatcaa
     1921 tggttaccaa cattgataaa gttgcagatg ttgaagtttt taattttgat gaaattccag
     1981 gacctcacat aacaccttat agaatgatgg aattgaaaaa ttatgtaaat gatttattag
     2041 caagaaaaga tattacaggt gttgtaataa cacatggtac agatagttta gaggaaacag
     2101 cttattttct agaccttaca attgacaaca tcaaacctgt aatagtcaca ggtgctatga
     2161 gaagtagttc tgaacttgga tatgatggtt caagtaattt atctgcatca gtttgtactg
     2221 cagtatccaa agatgctatg ggaaaaggag tccttgtagt tttaaacaat gaagtattat
     2281 tagcttctga agctactaaa acaaatactc tttctctaaa tacatttaaa tctctaacta
     2341 gtggcccatt aggaataatt gactgcaacg aacttgtcct tacaagagat attgtaaata
     2401 gaacaattat agatactgat aaggtagaat ctaaagttgc cttatttaaa gcatatgtag
     2461 gagaaaatgc tgactttata agatttgctg ttgatagtgg ttataaaggt atagttattg
     2521 aggcaatggg tagaggtaat attcctcctc aaatgctttc tggagttgaa tatgctagag
     2581 aaaaaggact tcctgtagtt atagtttcta gatgtcattc aggtagagta ttcgatagtt
     2641 atggttactt tggttctggt agagatttaa aaaatcttgg ttgtatattt ggtggggact
     2701 taccaggtca aaaagctaga ataaaactta tcttagcact tggaaaaact gataaccttg
     2761 atgaaatcaa agatttcttt gaaaaaggaa tctattgtta attctaataa cttctattta
     2821 attatttatt gatatttcaa catttttaaa tcaaaatcta atacttatgt cagttaaatg
     2881 tcagcaaaat attttatcca caagttttcc acaacttatc cccaatatta tccacaagaa
     2941 aaaaggtagc acttatatta gtagctacct tttatttatt aatcttcatc tttccagctt
     3001 tttatccaat ttcctatttc tattattttt tcttcagtta tttcttctcc atcatgcaat
     3061 tttgaaatta attctgataa tggattgtta tgtagacctc caaaaattct ttttccctct
     3121 attttgagat attctttctc ttttattaat actctatagt gtacatgttt cccaattctc
     3181 tgcgatatga aaaaaccttt tttagttaat ctagctaaaa gtgtaattgt agttgtatgc
     3241 ttccaagcat atgttttttc tatttcatct gcaacatctt ttgatattac agttttataa
     3301 ccagtattct atatgtattt cataattttt aattctgcat ctggtaattt tttattcaac
     3361 ataatatatt ccttctttca atttatgtca cttcctcaaa cttatctagc ttaaattaat
     3421 aataggagct ttttatcaca aaaactacag gtaaattttt ctatatattc tacgtattta
     3481 gtctgttttt taagtgtgaa attacgttca tacctataaa tttattcaat tttcaaacaa
     3541 tcttattgaa taataactaa ttctattgta ttatttagtt atagaataaa actaaatcgg
     3601 caaaactaga gaaatctagt gacgcaaagc tatagagact aagacttata taaatatctt
     3661 atgagttatg tcagccagtt gccaaagaga tattgttctt tttgttttta tgaaagtttt
     3721 ttagggggat aataatttat atatttagag ataaaataga taaatgtaca catatattga
     3781 ctgcttatat tagcagttcg tatgattatt gtaatttttt ggatacaatt tacattccat
     3841 atagttaata taaaacaagt gtttcgaatt tcttttctga aactatacca acaatttaca
     3901 ttccatatag ttaatataaa acgttatata ctgaaagcgg tgaaactggt tacgtagcaa
     3961 tttacattcc atatagttaa tataaaacct gtgcgtgtag gagatggtaa cacaactgtt
     4021 gaagaattta cattccatat agttaatata aaacacacat ctacatgttt ttctatctga
     4081 gttcatctct aatttacatt ccatatagtt aatataaaac tttttaatcc ttcttctgaa
     4141 ctcccaaagt ttatatcatt tacattccat atagttaata taaaacccac gaaatttaag
     4201 gtcgatatat cagatttgaa aaaatttaca ttccatatag ttaatataaa acaagctaaa
     4261 tggagcaacc gaccaagtcg ctagaaataa tttacattcc atatagttaa tataaaactt
     4321 ttctattaag tgtaagcaga atgcatggaa tatgaaattt acattccata tagttaatat
     4381 aaaaccccaa aataaattga gtatttccaa tgcctacaca tatacaaatc tctcaaattt
     4441 gcagtgaacc atgagtagtg caattgataa cacttatcac aagtcctcaa tgccttatat
     4501 ttcaactgtt aagccttaat ttcttcaaaa aatcgaacac tgcaaaattt ctatgacttt
     4561 attatatcat aaaaatatta tttttgaata tctgtaccaa tttgtgatat aataaaagca
     4621 agaagaacta caatctattt tgctgtagag tggagttcat aatttagaaa ttacttagtt
     4681 ttatggaatc tgatttttaa atcaaattcc caaccactct tagttgccgc tttgagtggt
     4741 tttttacgct ttctaaatag tttgctagct atataaacta ttaagctagc agatagactt
     4801 gctagtatgc cttgtaaaaa attatccatg tatattcacc tcccttctta acgtcgggag
     4861 gataattttt tgatacatga actccactct ataaattgta gattacatct tcttgctaaa
     4921 aatattataa catataattc ttacatattt tacctattta ttacttttta cattataata
     4981 atattctttt aatcaaaaaa acacctacta ttaaagtaaa tgtttttaag tatttttaat
     5041 tttaaaatcc atatagttca tatgaaacta aatataataa ctcaacatat gagcaatatc
     5101 gagacattta catacccact agttatttat attcaaaaaa atcttctcca tccgggttat
     5161 aaaataatat tctacttgcc aatccttgtt catatttata attttcttta tcacctacat
     5221 aagaaaatgt taatgtcatg cttcttgatg gatctaatga aattatatct aaataaatag
     5281 tacatttttc tcctggcttt aatacaccga aactattaaa atccattttt attttagcgt
     5341 attgccccat atataatgtt gaattagctt ctccataaac ttttaatttt gttacatcta
     5401 aattttgtgt acttgtattt tctatattta taggaactgt tgggtcttta tatttttcag
     5461 tataatattc aaggttatca tcataacatt caagataact tcctttatct tgccaaatta
     5521 tgtgatttga atcaaaatca atctttaatc cacatattat atttttattt gaagatggaa
     5581 gactaatatt attgatggta ttttcacact ctagcataat tttatcactt agatttccta
     5641 tcccggttac ttttgcagca tttttaagta tatctttatt acttccaata tcaactagta
     5701 caataggatt atttttagcc attggtgaag caactaaagc ataaactaaa tcagtaccac
     5761 tagctatata aaattctttt gcgccattat agaacttatt tactattttt ttattagtat
     5821 catatctatc tactccaccc aatcgagttg aattagtatc atttactaaa ctatcactca
     5881 ttgatgaagt gccacctatt gcataacttt ctataccagt tgtattaaat ggaacacttt
     5941 ttccatcagt taatactata gctgctttat ctctaactgc tacaggtgca gcactcatag
     6001 catctggctc acctttgaaa gcgttagtta atattacttt atttacttta ttaatggaat
     6061 ttatttcttt tgctacatta taacttgtct taatcctatc attaccttga agccttctag
     6121 tctctattcc tttatctttt agaaaagttt ctgttgcttt atcaatggaa ctttctcctc
     6181 ctattatgta taccttttta gctttttcta ctcttttaag agttgcatta ggtatattat
     6241 tctttttagt caaaaggatt ggtgcatttg ttgctcctgc aagtccactc gcacttagcc
     6301 catcagccat tgtactatct gcatttataa gtattgcagt agtataactt tgtttgtcag
     6361 ctatgatacc agcagtttcg tatttatctg ctccttgaat tttatcaatt ttatcaagtg
     6421 cattagctga tataggaaaa ccagcaacaa acattgacac taaccctagt gataataatt
     6481 tctttaattt cacaatatct ccccctttta tatatgaaat actatatatc ttaataattc
     6541 tacattaaat tttcttttcc tcctttaacg atatttatct tcctgttaat ttaagtgtat
     6601 acaaatacgt gttataatgt aactgtaaag aattaaaaag ataatgacat aattagagta
     6661 gtacaactgt tcttttataa aactaactta taaagggagc gtacatatat gaatatcaaa
     6721 tcagctttta taagaaaaag aggggaaaaa ttctatgtat atgtggagta catagaggaa
     6781 acaactggca agataaaaca gaaaagttat ggaagttatg aaaagaaaaa ggatgctgaa
     6841 aaacatttaa ttgaaataaa atctactata aacaataata agtttgttgc tccaaaagat
     6901 atgacatttg tagaaagatg ttataaatac atggacatga ataaaaataa ttggtctcca
     6961 tatactgtta caaatacaaa atcttgggtt aaaaattaca tagaacctct ttttaagaat
     7021 ctaaaactca cagaagtaaa tcctagttca gtacaagctt ttgtaaatta taactttaat
     7081 aattctacct ttactagtgc taaagtaagg tatagctttc taagttctgt aataagggaa
     7141 gcatatagat taagagaaat acaagaaaat tcatgtgatt ttataaaact tccttctaaa
     7201 gaaaaatatt ctacaaatga aatatacaat agaaaagaag tattattgct aatagaaaag
     7261 ttacttagta ataatataga aatacctatt cttttaatgt tacttctagg tttaagaatg
     7321 ggagaagcta ctgggcttag gtggtcagat gttgacttag ataacaatct gataaatgtt
     7381 aatcaaattc ttgtctattc taatggcaaa atatctttta aagagccaaa aacaccaaaa
     7441 tcaaaaagag ctttatcagc tccaaaagaa ttaattgaaa aattgaaaat taaaaaacta
     7501 agacaaagca aattaaaatt gtaaggtatc ctggaaaaca gaaataattt agtttgtcta
     7561 aacaccaatt taaatccgtt tctaccttct tcactaagaa aaacgtttca caattttaca
     7621 aaaaaaataa tttaaaaaag attagaatac atgatttaag atatacaagt gccactctgc
     7681 tgcttttagg gggaactaat atgaaagttg tttctgaaag attgggtcat acagatataa
     7741 aaataactat gaatagatat tctcatgtac ttaaagaaat ggacaaagaa gcttccgaca
     7801 atctaagcga acttctattt aaatagtaaa aactgactgt cagttaaatg tcagttaaac
     7861 ctaaaaaaat aggttttgtc agttgattat cagtcaatta ttttataata gtttcactta
     7921 aaaacgtcta tatctacaca ttttactatt tgtttatgct tattaatatc tatctgttat
     7981 tattaattct aatagcttct atttaattat ttagttcagt ttacaggaaa ctacacacaa
     8041 accataaaat aggatgttga caatataaca gatagtatgc tcctgtaaaa ggtaacgtac
     8101 tatctgttat gttgtctgga tttccaaatg gttacccttt ggcacacaac tttgctatct
     8161 ccttggtaaa ctaaaagttt gtaatctaaa atcctaatcc atctctataa aatttaattg
     8221 acttctccat actttttaca cctaaacata taaaagtaat tttattcact taatgacctc
     8281 cataattccc aaattagcta tttggaactt aactaaaaaa tcctaatata agggcaacta
     8341 ttataatggc aatcggaata catactgtgt aatcctgttc catagagtca tcatctgaat
     8401 gtagttttac aattcgcaat ttagcaatca gtttgaagca gaaatagcct attattgttc
     8461 caattacatt cgtgagtaag tcgtctatat cggttgctct gtgtaacgta aaaagctgaa
     8521 tagtttcgat aaaaagagat aatccaaaac caatcaagaa tgtatttctc aaacgttcaa
     8581 aggttttgct gataagcgga cataaaaaac ctaatgggac aaaaagtaaa atattcaaaa
     8641 taaaactcaa actaaatcca tcactaaaag gaataagatt tatgtttgga ttaaagaacg
     8701 tttcccctaa ctgtgacagt cttatatatt cactgaatgt agggatacca acaatatgcg
     8761 atagtgttaa acataagtag taatacattg ccagtgataa aatagatgtt tttacctttg
     8821 gaatatcact atttttcttg aatattacaa ctaagaaaat accaatcaag ataattccca
     8881 acaagaaaat aggattaatt aacaataaat ctttgatagc ttcaaacaga ctgagttgaa
     8941 tttctacacc tgattgatta tttgttacag tctccatttg tacatacctc ctcataacat
     9001 ttcaattatt tctaaaacac aaaatagaag ataaatctta aaatcatcct atcaaaattt
     9061 ttaaatttgc ctttatcagt ttataccacc agttaattcc tgtcttactt ctgtatggta
     9121 ttcttgggat tctttcatca aattagacaa aaaatggtta ccatatcaat aaactggtta
     9181 cgctccatat ctcccgaagc aaccatcccc tgagaataaa tccgtatcag gaatctccac
     9241 atctactgca cctatcacaa gttatttgat aacatcctca gacaatccca acttggatat
     9301 atcgacaaca cccattagaa taccactgct gaccatactt gttttcctgc tgataatttt
     9361 aatcaactgg gaagttgaaa cgctatcttt tttacaagtt cctttttaga aacatggtag
     9421 ctattatatg aatctgaatt tcgttgtctg ggtgtttcat ataaagcata gatacgcacc
     9481 ttttttgcct gttttcatta tatagaaaaa aatttcaaaa tacaataatt atattttttt
     9541 cgtttaagct tagagcagtt ccttgatgaa caacactttg gaaactgaat gatttacata
     9601 ccacttaata cattaactcg atttaacatt aattataata atatttaaaa caacaactag
     9661 ggtatataag aatgaacttt ttagttcttt aatcattttt ttgtataaaa tcaaatctat
     9721 ttttataatt tcaacaattt tataatttca acaatgaaaa aaggcttatc tctattttga
     9781 gacagcctcg tttattttat aaatttatat ttttgttcat atttttgagt tatttaacaa
     9841 ctatatttac tagtttttta ggtacagcta caactttaac tatagtttta ccttcaacta
     9901 atccttttat tttttcatct tctaaagcta ccttttccat ctcatcttta gatatatttg
     9961 agttaacagt taattttcct ctaacttttc cattaacttg tacaacaact tctatttcat
    10021 cttttactaa agctttttcg tcatatttag gccagtctat gtcaaataca cttccttctt
    10081 tacctatcat agtccatagt tcttcaccta aatgaggggc aaatggtgca attatagtta
    10141 ctatagtttg tacaccttct tttataactg cttcgtttat attatctaac tctttatatt
    10201 tatacatatc atttattaat tccataagag ctgatatagc agtattaaat ccgaattttt
    10261 cacttaaatc agcagtaact tttttaagtg tagagtgtat agtatatctc atatccttat
    10321 cttgggaatt taattctcca aactctacat cctttttaac cacatctgct aactcgtcaa
    10381 ccaatctata aaccctattt aagaatctaa agcatccatc aacaccttgc tctgaccagt
    10441 ccaaatctct ttctggtggt gctgcaaata atacgaaaag tcttgcagta tctgctccat
    10501 actcatctat tatatccata ggagatactg tattcccttt agacttactc attttagaac
    10561 catccataag taccattcct tgagtaagta agtttttaaa tggctcattg aagtctacca
    10621 ttcccatact cttaaatgcc tttacaaacc atcttgcata aagtaaatgc attatagcat
    10681 gttctactcc acctatatat tggtctactg gatgccatct attaactaat tccttactaa
    10741 atggttcatt ttcattattg ctatccacat atcttaagaa ataccaagaa gaatcaacga
    10801 atgtatccat agtatcaact tcccttctag ctggttttcc acagtgagga caagttgttg
    10861 acataaattg ctttgaagtc gtaagtggag attccccttt tccagtaaat tcaacatctg
    10921 ttggtaaaag aactggtaaa tcttcctttt taactggaac tattccacat tcatcacaat
    10981 aaaccacagg tataggacaa ccccagtatc tttgtcttga aactaaccag tctctaagtc
    11041 tataattaat tgttctcttt cctctacctt ctttttctaa cttatcgatt attccttcaa
    11101 atccttctga agcatctaat ccattaaatt cttctgaatt tatcattttg ttatcttctg
    11161 ttattacatc aattatgtct aaattatatt tttcagcaaa atctctatct ctttcatcat
    11221 gtgctggaac tgccattata gcacctgttc catactcaac taatacatag ttagctatcc
    11281 atagaggaac tttcttgcca tttaaaggat ttataacata tctacctata aacattcctt
    11341 ctttttctac atcagctgct gttctttcta tttctgtcat agtatgcatt ttttgaacaa
    11401 aagcttcaac atctgcttca tattcagtcc ctgcaaccaa ttctttaact aattcatgct
    11461 ctggagcaag taccatataa gtaactccat aagttgtgtc tggtctagtt gtaaatactg
    11521 tcatagattt atcagttcca tctatatcaa aaactaaatc tgctcctgta cttttaccta
    11581 tccaattttt ttgcattgtt ttaacttttt ctggccatcc atctaatata tctaagtcat
    11641 taagtaattc ttctgcaaat tcagtagtct taaaatacca ttgttctaaa tctttcttta
    11701 atactgttgc tttacatctc tcacatgctc cttgaacaac ttgctcattt gcaagaacag
    11761 tctcacaaga aggacaccag ttaacatatg attttttctt atatgcaagc ccatgctcta
    11821 aaaacttcaa aaatatttct tgtgtaaatc tatagtattc tggcgttgaa gttgcaactt
    11881 ctttatccca atcataacta atacctaata aatttaattg ctctttcatt tcttctatat
    11941 tttccattgt ccatttatgt ggatgtattc catgttttat agctgcattt tcagctggta
    12001 atccaaaaga atcccatccc attggatgaa gtacattata tccttccatt tttttgaatc
    12061 ttgcgacaac atcacctata gaataatttc taacatgtcc catgtgtatt tttccagatg
    12121 gataagggaa catttctaat gtgtaataat taggtttttc agtttgtgca gtatccattt
    12181 tatactgatt attatctttc cagatttttt gccatttcga ttctacttct ttaaaattat
    12241 atacgctcat attttgcctc ctatttttaa ttgtgtataa ctaccttatt tagtattatt
    12301 tacttataat ttccaaataa ttctttgaaa ataaaaaatc ctcgtcacta aacttacgtt
    12361 tagggacgag gataaatctt cgcggtacca ccctaattag cctactacat atatagactc
    12421 tcttagatag cttttaatcc agccatggag aatagatttt aatctagtaa gttcaaagtt
    12481 atataatatt
//
LOCUS       DAELTA010000104        12478 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.104, whole genome shotgun sequence.
ACCESSION   DAELTA010000104 DAELTA010000000
VERSION     DAELTA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.104"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            185..1576
                     /gene="hydA"
                     /locus_tag="KPG13_002530"
     CDS             185..1576
                     /gene="hydA"
                     /locus_tag="KPG13_002530"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG0986235.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            1570..4131
                     /gene="xdh"
                     /locus_tag="KPG13_002531"
     CDS             1570..4131
                     /gene="xdh"
                     /locus_tag="KPG13_002531"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG0986236.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            4283..5629
                     /gene="rpoN"
                     /locus_tag="KPG13_002532"
     CDS             4283..5629
                     /gene="rpoN"
                     /locus_tag="KPG13_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG0986237.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            6411..7433
                     /locus_tag="KPG13_002533"
     CDS             6411..7433
                     /locus_tag="KPG13_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0986238.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            7493..8500
                     /gene="gap"
                     /locus_tag="KPG13_002534"
     CDS             7493..8500
                     /gene="gap"
                     /locus_tag="KPG13_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG0986239.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            8722..9924
                     /locus_tag="KPG13_002535"
     CDS             8722..9924
                     /locus_tag="KPG13_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG0986240.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            9950..10693
                     /locus_tag="KPG13_002536"
     CDS             9950..10693
                     /locus_tag="KPG13_002536"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG0986241.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            10702..12234
                     /locus_tag="KPG13_002537"
     CDS             10702..12234
                     /locus_tag="KPG13_002537"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG0986242.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         5070 a         1411 c         2497 g         3500 t
ORIGIN      
        1 tctaaagtta ataattttat tatattgatt taactaaata ttagtttagc tgaatattaa
       61 tttaattagg tattggttta atcttaatat agatttactg cgtataagtg aactaactag
      121 attactttaa gttaattttt tcatataaaa taaattgata cattacaagt aaggtggagt
      181 ctatatggga attgttttaa aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc
      241 agatgtaaga atagaagatg aaaaaatagt cgatataggt cttgatatat caaaagaaaa
      301 tgatgaaata gttgatgtta gtgattctta tgttatacca ggaggtatag atacacacac
      361 acactttgac ttagatacag gcgttactaa gacagcagat aactttgaga caggaacaaa
      421 agctgctata gtaggaggaa ctactactat attggatttt gctacacaag ctaaaggatg
      481 ttctttgaaa gacggattag aagaatggca tgaaaaagct aaggataaat cctattcaga
      541 ttatggcttt catatggcta taacagattg gaatgactcg gtttgtaatg aaatggagga
      601 tatggtgaaa gagggagtat cttcatttaa actgtatatg gcgtataaag gaagccttca
      661 agttgatgat ggtgtaatct ttgaagcatt gagaaaagca gaagagatag gagggattat
      721 tggtttccat tgtgaaaatg gggacataat atgtgaactt gtggataaag caaagtcaga
      781 aaataattta tctccaaaat atcatcaatt aactagacca gctataatgg aggcagaagc
      841 tacatcaaga cttatgaaga tagctgaagt tgcaaactct aaagtatatg tagttcactt
      901 aagctgtaaa gaatccttag aggaggtttt aaaagctaag caaagaggtg tggatgtaat
      961 agttgaaact tgtcctcaat accttctatt aaatgatgaa ttatatgatt tagatggatt
     1021 tgaaagtgct aagtatgtaa tgtcaccacc tttaagaaat aaagaaaata atgaaatatt
     1081 atggaatgct attttagata atcaaataga cactatagga acagaccatt gttcttttaa
     1141 ttataaggga cagaaggaac ttgggataaa cgatttcagt aagataccaa atggttctcc
     1201 aggtgtagaa cataggatga gtcttttata cacatatgga gtctgtaaaa ataaaatatc
     1261 tatcaacaag atggtagaag taacatcaac taatgcagca aagttgtttg gaatgtatcc
     1321 taataaagga actatagaaa taggcagtga tgcagatata ttagtcttaa atccaaacaa
     1381 aaaatccaaa atagaagcaa aaaaacaatt acaaaatgta gattatacac cttatgaagg
     1441 gtatgaagtg gattgtcaaa tagaacatgt ttatttaaga ggaaaagaag tagttagaga
     1501 tggagaatta atttgtaata ctccaagtgg gagatatata aataggcgtc taacaaaggg
     1561 ggctagagaa tggtagaatt tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa
     1621 aaacttttag acttccttag agaagatatg aatataactt cagttaaaaa tggatgttca
     1681 gaaggtgctt gtggaacatg tatggttata atagatggta aagctactaa agcatgtgta
     1741 tttaaaactg ataaattagc tgggaaaaac ataacaacta tagagggatt atcagagaga
     1801 gaaagagatg tttttgcata ttcttttgct agtcaaggag ctgtacaatg tggattttgt
     1861 attccaggta tggtaattag ttcaaaagca cttatagacg ttaataataa tcctactgaa
     1921 gatgatataa agaaagcttt aaaaggtaat atatgtagat gtacagggta tgtaaaaata
     1981 attaaagctg tcgatttagc ggcaaagatt tttagagaaa atatagatgt gccaaaagta
     2041 aactgtaagg gtctagtagg tgaaaatctt cctagagttg atgcagttgg caaagcactt
     2101 ggatattctg aatatgtaga tgatatgaga attgagggta tgatttatgg ttcagcagtt
     2161 agagctaagt atcctagagc tttagttaaa aaaatagata tagaagaagc aaaagcttat
     2221 ccaggagttg ttgcagttct aacagctaaa gatattccag gaactataaa ggtagggcac
     2281 ttaaagaagg attgggatac tttaatacca gaaggtgaaa taactagata cttaggagat
     2341 ggaatagttt tagtagcagc agaaactaga gaagcgttag aagaggccaa aaagctagtt
     2401 aagatagatt atgaggaatt aacaccatta agtactacag cagatgcact tagagaagat
     2461 gctccaaaga tacatgagac agggaatata cttgttaagg aacatttagt aagaggaaac
     2521 gctaaagaaa agatagaaaa ttcaaaatat gttgtaacta aaagatatac tacacctgct
     2581 acagaacacg catttttaga gcctgaatgt gcagtagctg gtccttatga agatgatgga
     2641 gtaataatct attcgacaga ccaaggggtg tttgccactc aacatgaatg tgcagatatg
     2701 ttagggttac catatgagaa ggtaagagtt ataaacaaaa tggtaggagg agggtttggt
     2761 ggaaaagaag acatgagtgt tcaacaccat gcagccttac ttgcatacca tacaaagaga
     2821 tttgtaaaag tacagttaac aagaaaagaa agtattattg ttcatccaaa acgacatgcc
     2881 acagaaatgg aatttacgac agcatgtgat gaaaatggat acttaacggg tatgaaggca
     2941 acgataatat ctgacacagg tgcatatgcc tctcttggag gaccagtgct acaaagactg
     3001 tgtactcatg cagcaggacc atacaattat caagatattg atataacagg aacagcagta
     3061 tatactaata accctcctgg aggagcattt agaggatttg gagttacaca gagttgtttt
     3121 gcaactgaat gtaatctaaa tttacttgct gaaatggttg gaatatctcc ttgggagata
     3181 agatataaaa atgcaataag acctgggcaa gttttaccaa atggtcaatt tgcagatgaa
     3241 ggaacagcaa tggcagagac actagaagct gtaaaagaag agtatgaaaa gcatccacgt
     3301 gcaggtatag cttgtgctat gaaaaatagt ggggttggag ttggtatacc tgatgttgga
     3361 agatgtagat tggtgataaa agatggcaaa gttcacataa gaacaagtgc ttcttgtata
     3421 ggtcaagggc ttggtactgt tatgactcaa gtagtttgtg aaactgctaa cctacttcct
     3481 gaaaatatag ttcatgaagt tccagatact catttaacac cagactctgg taatactacg
     3541 gcttcaagac agacagtatt tacgggagaa gctactaagg ttgcatcaaa taagttaaaa
     3601 gaagctttag actctggcaa aactttactt gatttagagg gagaagagtt ttatggagaa
     3661 tatgaaagta ttacagataa aatgggttgt gacaaagaaa atccagtaag tcatgttgct
     3721 tatggatatg caacacaagt tgtaattctg gatgaaaatg gaaaactaga aaaagtagta
     3781 gcagcacatg atgttggaag agctataaat ccaatcagtt tagaaggtca aatagaaggt
     3841 ggagtagtta tgggtcttgg atatgctcta accgaagatt acccagtagt taactctatt
     3901 cctacagcta agtttggtac attaggttta tttaaatcta cagatatacc agaaatacaa
     3961 gctttaatag ttgaaaagaa caaagattta ttggcttatg gggcaaaagg tgtaggagaa
     4021 atttgtacag ttcctactgc accagcagta caaaatgcct attatgtatt tgataaagaa
     4081 tttagaactg agttaccact acaaaatact ccatatagta aaaaaaagta acaaaaaaaa
     4141 gaaatattaa ataaaaaaat agatgaaatt gtaaataaaa aaaacccttt tgcataaggt
     4201 atattttaaa tgatagaata tacatataca ggagggtttt tcttttataa aataagttta
     4261 ataaaattga gaggtgagca gaatgaattt taataatagt ctagaattaa ctcagtctca
     4321 gaagctaata atgactacac aacttaagca atcattatca atattaaata tgagtaagtt
     4381 agagttagaa gaagagataa aaagagaagc agagaataac ccattagttg aagtcgagaa
     4441 aagtggggag ataaattggg aagagtatat aaaagacatg gataggtcaa gacgtttaga
     4501 tagaacagag ataagctata atcctgataa tgaagttaat ctagaaaatt tagttaaata
     4561 ttcgtcaaat ttatatgaag atttaaaatt tcagataagc ctgtataaac ttacagacaa
     4621 agaattggaa gtctgtgagt atataattga tagtttagat gaggatggat atttaagaac
     4681 tgaggaaaaa gtaattgttg ataccttaaa gatagatgaa gaactttttg aaaaatgttt
     4741 aattagtgtt cagcagttgg aacctagtgg agtaggggca agaagtcttt cagaatgtct
     4801 gatgatacaa atggctagtt taggaatata taatgaaatt ttagaggaaa tcgttacaaa
     4861 agacttaaat ttaattgcaa ataataaata caaagagata agtaaaaagt acaatatgtc
     4921 attacaaaaa tgtgttgaac taataaatat aattaagaca ttagacccca aaccaggaag
     4981 aacttgttca gtagagaaaa gtgtatacat tcaacctgat gtaacagtag aaaaaattga
     5041 tgatgaattt attgtatatt taaatgagaa agattcttat caaataagaa tcaatagtta
     5101 ttataaagac atattaaaaa attcacagtc tgatgaaagt gcaaaagaat ttataaaaga
     5161 aagactaaat tcagcaacag gacttatgaa aaatatagag agtaggaaaa ctacagtact
     5221 taagattgca gaagagatag taaaagctca agatgaattt ttgagaaaag ggacaaaata
     5281 tataaaacct ttgaaaatga aagacatagc agaaaaatta gaatttcatg aatctactat
     5341 aagtagaggt gtaaatggta aatatatgct aacacctttt ggcgtatatg aatttaggta
     5401 ctttttcagt agcgccattg agactgagaa taatgaaatg gcatctagta caagcataaa
     5461 aaaaattata aaggaaacaa taaaagacga aaataaaaag aaacctttaa gtgatgacca
     5521 tatatctaag ttattaaaag aaaaaggaat caatgttgca agaaggacag ttgccaaata
     5581 tcgtgaagaa ttaggaatcc catcttcaag taagagaaaa gaatactaat aagtaaaaga
     5641 caaatatcag tatggtaagg ggaaaaggct aattaagaga tgaatgtaga ttgggctaaa
     5701 aattttataa agtaaaataa tataatatga aaaagtatta aaatgctata aggtattaaa
     5761 aaactgggga acattaaaac attaagatag caaagagcat aaaaaactaa agaagattaa
     5821 aacattaaag aacattagag ctttgaggaa tattagaact ttaaagaata ttaaaattta
     5881 taaaatataa aaatactaaa aagaattgaa aaactgggga acattaaaat attgtaaaat
     5941 gctaaaaaac tagaaaacat aattaaataa gtgcttataa agaatgatta aattaaataa
     6001 gtgtttgtaa agaataatta gatattagaa aaagaagaat acctagataa ataaactatt
     6061 attaaattta ataatcacaa aaggaaaaca cgtagggtat aaataaaaat cttaactaga
     6121 taaaagtaaa cttagttgta gataaaaata actagaaata aaattattta tagtaataaa
     6181 tattagaatg aaaatataaa aaaatatata taaaaagcct tatataaaaa gaaaaaatac
     6241 tgtgatttga aaatggtgga gaaatatttt ataaaataaa aaaataaaaa agtttttgaa
     6301 aaactattga aatctatacg caaatcctat aaaataaaaa tatcttgtgg gacttaaata
     6361 aacactaggg acaaaaaaag tcccgaaagg aaagcaataa ggagtatgtt atgaagaact
     6421 tattagaaat tcaaaagaaa ttgattccac aggcaattga acttatggaa agaagatact
     6481 ctattttaag acaaatatct ttaagtgaac caataggtag gagaacatta tcaaatgtat
     6541 tagagattag tgaaagaata atcaggtcag aaactgagtt tttaaaagaa caaggactta
     6601 tagacgtagc tgtatctggg atgactatca ctaaagaagg ttctgatttg ttagataaac
     6661 ttaaggacat catgagtgac atcatgggat tgtctaagtt acaagataga gtcagagaaa
     6721 agcttggaat aaaaagggta atattagttc ctggaagctg tgatgaaaat ggaagtttac
     6781 taaaagatgt aactagatat ggatgcgaat atttcttagg cattttaaaa gatggagata
     6841 tcgtttcaat aacaggagga agcactatgt tggagttttc aaacgttatt aaaactgata
     6901 agagatatcc aaactctaca atagttccag ctagaggaag tatgggaact gatgtagaga
     6961 tgcaatctaa caatatagtt gcttcaataa gtaagaaatt acattctaat tgcaaacttt
     7021 tacatatacc agatgagttg gatgaaagtg caatgaaaac tcttagccaa gtgccagaga
     7081 ttaagacaac tttggagtat attaaaagaa cagacatttt agtttttagt ataggtagag
     7141 cagatgtcat ggcaaagaga agaaaacttc cagaagacaa ggttgatgag atattatcta
     7201 aaaatgcagt tggagaagca tttggatatt atttcaacaa agatggagaa atagtttaca
     7261 aactgaacac tgttggaatt gatttagaaa cagttaaaca agtaaaagaa actattgcaa
     7321 tctttgcagg gactgaaaaa gtagaagcct tattagctat atcaaatatg cataaaaaca
     7381 tggttcttgt aacagatgaa gaaagtgctt ataagatatt gtcattatta taaaagtcaa
     7441 gacctagcac agagctagaa aacatacatt tactttatag gaggtagcga aaatggttaa
     7501 agtagctata aatggatttg gaagaatagg aagattagca ttaagaaaaa tgatggagca
     7561 acaagataag ttcgaagttg ttgcaataaa cgacttaaca gacgctaaaa tgttagctca
     7621 tttatttaaa tatgatacag ctcaaggaag atttaacgga gaaatagaag ttaaagaagg
     7681 tgcttttgta gtaaacggaa aagaaataaa agtaacagca gaaagaaatc ctgctgacct
     7741 accatgggct gaattaggag tagatatagt attagaatgt acaggattct ttacatctaa
     7801 agataaagct gaagctcata tacaagcagg ggctaagaaa gttgttatat ctgcaccagc
     7861 tactggagat ttaaaaacaa tagtattcaa cacaaacagt gacatattag atggttctga
     7921 aacagttata tcaggagctt cttgtactac taactgttta gctccaatgg ctaaagtatt
     7981 aaatgataaa tacggtatag aaaaaggctt aatgactact atacatgcat acactaatga
     8041 ccaaaatact ttagatggtc ctcatccaaa aggtgattta agaagagcta gagctgctgc
     8101 tggaaacata gttccaaaca ctacaggagc tgcaaaagca ataggcttag taataccttc
     8161 attaaaagga aaattagatg gggcagcaca aagagtacca gttgtaactg gttcaataac
     8221 tgaattagtt tgtacattag gaaaaaatgt tacagttgaa gaaataaatg cagctatgaa
     8281 agaagcttca aacgaatctt tcggatatac tgaagaaatg ttagtatctt ctgacataat
     8341 cggaataagc tacggttcat tatttgacgc tactcaaaca aaagttatgg aagtagatgg
     8401 aaaacaatta gttaaagttg tttcttggta tgataatgaa atgtcttata cttctcaatt
     8461 aataagaact ttaggatact ttgcacaatt agcaaaatag tcagttttga gaaaatagaa
     8521 aagtagattg acaaagtccg agtttgtagt ttaatactac ggactcagga ctttttttaa
     8581 gaaaaaatat ataaaataca cctaaataat gttacatact atatataaaa atatgataat
     8641 atgatatatt taatagggta aaagtatgta ctatattata cttggcaata aaaaattact
     8701 ttattaaaag gagagatatt tatgtcaatg cttaacaaaa aaactataga agatattgat
     8761 gtgtgcggaa aaaaagtatt agtaagatgt gactttaatg tgccattaca agatggtgta
     8821 attactgatg aaaatagatt gaacggagct ttaccaacta ttcaatattt aatttctaaa
     8881 ggggcaaaag ttatattatg ctctcactta ggaaagccaa aaggagaagc taaaccagaa
     8941 ttatctttag caccagtagc aaagagatta tctgagatgt taggaaaaga agttgtattt
     9001 gcagcagacg ataatgttgt aggagaaaat gcaaagaaag caactgaaaa aatggaaaac
     9061 ggagatgtag tattattaga aaatactaga tatagaaaag aagaaactaa aaacgaagag
     9121 aatttctcta aagaactagc atcacttgct gaaatatttg taaatgatgc atttggaact
     9181 gctcatagag cacattgctc aacagttggt gcaggcgagt ttttacaaga gagagtttgt
     9241 ggatacttaa ttcaaaaaga attaaaattc ttaggagaag ctgttgcaaa ccctgtaaga
     9301 ccatttacag ctatacttgg aggagctaaa gtttctgata aattagctgt tataaacgaa
     9361 ttattagaaa aagttgataa tctaataata ggtggaggaa tggcttatac attcttaaaa
     9421 gctcagggtt atgaagtagg aacttcttta cttgaaatag ataaagttga atatgctaaa
     9481 gaaatgatgg aaaaagctaa gaataagggt gttaacttat tattaccagt agacgttgtt
     9541 atggcagacc actttgcacc agatgctact ccaatagtaa cagaagatgc taatgtaaaa
     9601 gaagattaca tgggactaga tatgggacct aagactatag caaactttgt aaaaactata
     9661 aaagaatcaa aaactgtagt atggaatggg ccaatgggag tatttgaatt tgaaaacttt
     9721 gcaaatggta cattatcagt agctagagct atggcagagt tgactgatgc aactactgta
     9781 attggcggtg gagatagtgc agcagcagtt aatcaattag gatttggtga caagatgact
     9841 cacgtatcaa caggtggagg agcatcacta gaatttttag aaggtaaaga attaccaggt
     9901 attgcagcat tagataataa ataaactaat atatagagga gattgtaaaa tgagaaaacc
     9961 tataattgca ggaaactgga aaatgcataa aacaataaaa gaagctttag aatttgtaaa
    10021 tgaagttaaa gataaagtaa attctgataa agtagaagct gttatatgtg caccatttac
    10081 tttattaaag gatttaaaag aagctactaa gggtacaaat ataaagatag gtgctcaaaa
    10141 tatgcacttt gaagaaaaag gagcatttac aggagaagtt tcacctctta tgttaaaaga
    10201 aatagatatg gactatgttg taataggaca ttcagaaaga agacaatact tcaatgaaac
    10261 tgatgaaact gtaaacaaga aagttttaaa agcattagaa gtaggaatag acccaatatt
    10321 atgtgttgga gaaactttag aacaaagaga agctggaaaa actaaagatg tttgtaaagt
    10381 tcaagttgaa aaagctttag aaaatgttct taaagatgat ttagcaaaag tagttgttgc
    10441 atatgagcca atctgggcta ttggaactgg taaaactgca actgctgaag atgctaatga
    10501 cgttatatct tatataagag aagttataaa aggattatat ggagaattag ctaatgaagt
    10561 tagaatacaa tacggtggaa gtgttaaacc ttcaaatgta gccgaaataa tgggtcaaag
    10621 tgatatagat ggagctttag taggtggagc tagtttagct agtaatgatt acctagacct
    10681 tgttaatttc taaggagtga aatgatgaaa aaaccagttg ctttaattat tatggacggg
    10741 tttggataca ataaggatgt taagggaaat gcaattgctg agtccaaaac tcctaattta
    10801 gatagaataa aaaaagagta tccaaacaca ttgataaatg ctagtggtct tgatgttggt
    10861 ctacctgatg gtcaaatggg aaattcagaa gttggacaca ctaacatagg tgcaggtaga
    10921 atagtatatc aagatttaac tagaataaca aaatctataa aagatggtga cttttttact
    10981 aataaagttt tatgtgaagc aatggataat gcaaaagaaa actctcttca tgtaatggga
    11041 cttttgtctg atggaggagt acattcacat atagaccatc ttaaggctat aatcaaaatg
    11101 gcaaaagata aaggcgtaca aaaggtgtat gttcacgcat ttactgatgg tagagatact
    11161 gaccctcaaa gtgcattaga atatgccaaa gaagtacaag ctagtatgga tgaaattgga
    11221 gtaggtgaat ttgctacagt ttctggtaga tactacgcta tggatagaga taagagatgg
    11281 gaaagagtag aacttgcata caatgccatg gttagaggta ttggtgaaaa agcaaattct
    11341 attgaggaag ctattcaaaa ctcttatgat gatggaaaaa atgatgagtt tataatgcca
    11401 acagttatta tgaaagatga taaaccagtt ggaagtataa aggaaaatga ttcaattata
    11461 ttcttcaact ttagaccaga tagagctaga caaataacta gagctttagt ttgtgaagag
    11521 tttgatggat ttaaaagaga agatattaaa aacttctttg tatgcttaac agagtacgat
    11581 ataactatag agaatgtgca tatagcattt ggacctcaat ctttagccaa cactctaggt
    11641 gagtatctag ctaaaaatgg aaaaactcaa cttagagctg ctgaaactga aaagtatgca
    11701 catgtaactt tcttcttcaa tggtggagta gaagagccaa ataaaggtga agaaagacta
    11761 ttgatacctt ctccaaaggt tgcaacttat gatttaaaac cagaaatgtc agcatatgaa
    11821 ttgacagata aagctttaga taaattagga gaagataagt ttgactttat agtattaaac
    11881 tttgcaaatc ctgatatggt aggtcacaca ggaagtatag aagcggctat aaaggctgtt
    11941 gaaactgtag atacttgtgt aggaagattg atagataaaa tagtagagtt aggtggaagt
    12001 gcaataataa ctgctgacca cggaaatgct gaatatatgt tagacccaga aacaggaaaa
    12061 actgtaacgg ctcactctat taatccagta ccatttattg tagttggtca agaatatgaa
    12121 agtgcaaaat tattggatgg aggaagatta tctgatatag cacctactat tttagatatg
    12181 atgaagttag aaaagccaga agaaatgact ggtcattcac ttatttcaaa atagtcgatt
    12241 ctgataagta gataataata tataaactac aatataaatt taaaaataat gcagtttagg
    12301 tttattttgt aaagtttaga ctttatgaca taaggtttaa attttgtaag ttctggatat
    12361 tgtaaggtgt agatgaagat atattttata aaatttacaa gatagacaat ttataaaata
    12421 aaaaattaca atatagataa taatgtaaat aatgatataa ataataacaa gatagaca
//
LOCUS       DAELTA010000105        12449 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.105, whole genome shotgun sequence.
ACCESSION   DAELTA010000105 DAELTA010000000
VERSION     DAELTA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.105"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            10..567
                     /locus_tag="KPG13_002538"
     CDS             10..567
                     /locus_tag="KPG13_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0986243.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(806..1666)
                     /locus_tag="KPG13_002539"
     CDS             complement(806..1666)
                     /locus_tag="KPG13_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0986244.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            2165..3568
                     /locus_tag="KPG13_002540"
     CDS             2165..3568
                     /locus_tag="KPG13_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986245.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            3787..6264
                     /locus_tag="KPG13_002541"
     CDS             3787..6264
                     /locus_tag="KPG13_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0986246.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            6279..6965
                     /locus_tag="KPG13_002542"
     CDS             6279..6965
                     /locus_tag="KPG13_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986247.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            7239..8072
                     /locus_tag="KPG13_002543"
     CDS             7239..8072
                     /locus_tag="KPG13_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986248.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            8110..8571
                     /locus_tag="KPG13_002544"
     CDS             8110..8571
                     /locus_tag="KPG13_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0986249.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            8602..8850
                     /locus_tag="KPG13_002545"
     CDS             8602..8850
                     /locus_tag="KPG13_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0986250.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            9303..9623
                     /locus_tag="KPG13_002546"
     CDS             9303..9623
                     /locus_tag="KPG13_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG0986251.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            9761..10405
                     /gene="trmB"
                     /locus_tag="KPG13_002547"
     CDS             9761..10405
                     /gene="trmB"
                     /locus_tag="KPG13_002547"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG0986252.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            10534..11280
                     /locus_tag="KPG13_002548"
     CDS             10534..11280
                     /locus_tag="KPG13_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986253.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(11409..11687)
                     /locus_tag="KPG13_002549"
     CDS             complement(11409..11687)
                     /locus_tag="KPG13_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986254.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            11962..12408
                     /locus_tag="KPG13_002550"
     CDS             11962..12408
                     /locus_tag="KPG13_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986255.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
BASE COUNT         4795 a         1374 c         1875 g         4405 t
ORIGIN      
        1 ggattagaaa tgtctgattc tgaatatcct caaatgaaaa ccttagaaac tagaagttgt
       61 atactaagac ctgcatcatt agatgatgtt gaagattttt ttgactgtta taaaaataaa
      121 atagtagtaa aacatttacc atttagtgag cataaatctt tagaagatac aagaaagttt
      181 ataaaatcat tttttctaaa tccatataaa aaagggaaaa ttggccactt tgctatagta
      241 tataaaagag acaataaagt tattgggaat atggggttta ataatataaa tcctaaagca
      301 ttagaagcag aaataggtat atgtataaat ccaagctatt ggggacatga ttttgctact
      361 gagataacaa aaagagtaat acagtatgga tttagggaac taaacttaaa taaaataatt
      421 gcaataacct atgaagaaaa ccaaaattct acaaaatcat tagatttatt aggatttaaa
      481 tgtacaggaa agtatatcaa aaaaattcat actggaaata ctgtaaaaaa tgttccttgt
      541 tatcaatatg aattgaaaaa acaatagata taatcattta atatctatgc tggttattta
      601 gtaatgattg ttttctaaat aaaatttcaa taaagtggtt gcctcaaaat atagataatc
      661 ctgtttttat tgttaaaata ctgaaaatgc atttgatttt atacaaaaaa gagtgtctca
      721 tgaactaaaa agtttatgag acactctttt tgaataaatc gtattttaat taaaatagta
      781 tatatactta aaatattaat ttttattagt tctctaaatt tttaaatcct tctccaagaa
      841 cctcatgtac atctgtaaca ataacaaatg cagtagggtc agttctttta actaatctct
      901 ttagataaag ttcttgtttt ttagaaacta cgactaatag aacttcttta ttttctctag
      961 tgtatccacc tttaccatca agaatagtaa gacctctatc aagttcttca gttatagctt
     1021 gtcttaaact ttcaggctca tcagagataa taaaaaatgc ttttgaatgg tcaaatcctt
     1081 ccattatcat atctgctatt tttattaata cacaaagtgc tataccagag taaagagcag
     1141 tttctaaact acgatttact ataccagaag atataacaac cattccatct aggcatgaca
     1201 tgaattttgt agcttttatt cctgggaatt tcttactaag tatgagtgca attaagtctg
     1261 ttcctccagt agaagcatct gatttaaaca ttattcctaa acctatacca actaggattc
     1321 ctcctgcaat agaagacaat aataagtcat tagtaaatct tagttttgat aatgggtctg
     1381 ttaattgaac tatagctgaa aatacaactg ttccaaataa agtttttaat gaattttttg
     1441 tacccataat tctaaaagca agtattacta atgggacacc tataattaac atgatagctg
     1501 atactggaag tcctgtaacc ttgtttagta ctaatgatag accactaagt cctccaggag
     1561 caattgtatg tggttttaaa aacatattta taccaatact cattgatata caaccaaaaa
     1621 atagtccaag catttcaagt aaaattggcg acttttgttt tttcatataa acacctccat
     1681 gaacttgtca caatttaata ttataacata tttgaccagt ttatattata taaaatttct
     1741 tgatataagc aatttcaagt atttcattat tcttaatatt tttgaatttt tatatagtgt
     1801 tttcatatct ttactgatgt tgttacactc ctatgaagtt ttttatatat tataattttt
     1861 ttattctttt ataaagtatc ccctataaag atggttctat gaaaattttc tctatattgt
     1921 ttaacgtata tagagatttt gtgtataatt ataaggtatt taatggaaaa atttatctta
     1981 cttttatatg ttatcaaaat gatttattaa atacaacaga cagcttgata acatgtaatt
     2041 atataaatat aatttatgta gatagttgta tttttaaata aatttttaca tatcaataat
     2101 tattgtctat tttatataag caacaatcaa actaaaataa aagtttaaga aaagagttga
     2161 ttaaatgcat aaacctagtt ataaaacagt atttcattca tacattattt ttttcatgat
     2221 ttttatttgc ttaatcctga cagtaatagg tattttctta tcacttatta ctattcagaa
     2281 accaaatggg gaatttattc gtagtgactg gccgaaaaaa tttacagaaa actttaatga
     2341 acaaatcgta tttatagata ataagccaca agtaaaacag tctggattag aaaaacttcg
     2401 agaaaatcaa ttgtggttgc aaattttaga ttctagtgga aatagagttt atgggttttt
     2461 agaaccaaaa gagcataaag attattattc taattctgaa ttattagcat tagctaacca
     2521 aaataattca agtaatggaa ctgtatactt taatacaatt aaaaataata aaaatgacta
     2581 tatttatatt atatattttc cattaaatat atctaaagtg aatatgtttt ttgatggagg
     2641 taaatttata ggtggtaagt caattgtatt ttcaatcata gggattatat ttttaataat
     2701 tttggtttct ggaattgctt atggttactg ggtaacaaag tgcatatcaa atattacatc
     2761 tgctgttagt gatatttcaa aacgaagcta tcttccagtt aatattaaag gtgtttttgc
     2821 tgatatatat aatagtttaa atattttaaa tgaagaaatt cataaaagtg atgagataca
     2881 aaataaaact gatattatga gagaagagtg gatttcaaat ataactcatg atttaaaaac
     2941 tccactttca ccaataaaag ggtatgcaga gcttcttaca gatagtagta ctaattattc
     3001 acctgaaaaa gtatataaat atgcttcaat catgttaaag aacatagaat atacaaatac
     3061 acttataaac gatttaaaat taacctatca attaaaaaat ggaataatcc ctttaaataa
     3121 aaataattat gatttagtaa gattcttgaa agaattatcc attgatattt taaattatcc
     3181 agaatatgaa tcaaatatag ttgaatttaa gagtaatata gataatatta actttgattt
     3241 tgattgtaca ctattaaaac gagcttttag taatcttatt gtcaatgctt ttacatatgg
     3301 agataaaaat actaaagttt gtgtacatgt aacattaaat gaaagaattg aaatcattat
     3361 atctgataat ggaaaaggga tgtcaagaga agaagcatca aatttgttta atcgttatta
     3421 tagaggaagc aatacaaaac aaaaaataga aggaactggt ttaggactag caataacgaa
     3481 gcaaattatt gaagttcatg gagggaatat atctgttgaa agtatacctg gattaggtag
     3541 ctcattttat atcacctttc ctatttaatt aaggttaatt aaggttaaat aaaaaatata
     3601 ttaaggttga tagattatta tatttatata gtagtttatc aatctttttt atttaagggg
     3661 tgatttgatt aaaaatatta taaatagaat gataaaagta tggaggagtc tattagagat
     3721 ttaatttaga ttaaagtata aaaatattag attgaaatat cttagataag ggaagaaggt
     3781 gtaagtttga aaattatatt aaagtatatt ttcacaaata ttaaagaacg aaaaattaga
     3841 acagcagtta tgttattgtc catagtttta tcaacagttt tactttttgt ttcattctca
     3901 attggattat cttatgaaag tgcacaaaga aaaatggcaa aaggtatgta tggtacagcg
     3961 actatatctg tccaaagtaa aaatccagat atattaacca atttagaaga catacctgat
     4021 ttaaatacta taaaatcaaa ggttggtgta ttggaaagct ctgctattta taataaaggg
     4081 ggatactatg aagaatttag tcttatatca gcagacttaa gtcaactaaa taaaatcaac
     4141 aagccacgat tagaaaatgg cgatagtatt actgattttt ctggtgataa aattattctt
     4201 ccaaatcgct ttacatcaaa atataaaata aaaaaaggtg attctattac tttgcagatt
     4261 tatggaaagt cttatacttt tcaagttagt gatattgcat cttatgatac agtatttttg
     4321 aggaacacaa gaggtgtaaa tgcactgcta cccaaagaga ctctatcaaa aattataaat
     4381 aaaggcagtg gatatacaag agttcttata gagtcagaag aagaaattac agaaaatcta
     4441 gtaaataaat tatccgaaga actttctact gaaaaatata tggtatcaaa tactataaat
     4501 gaaactaaga taatttcaga tgctagacaa aagacaatgc ctttcttttt aataagtttt
     4561 tttgcattaa cccttagtat atttataata tacagcagtt ataaggttat tacattagaa
     4621 cgtcttcctt ttattggaac atttagaagt attggtgcca atgaaaaaac tgtaactcgt
     4681 atattgatgc ttgaaagtat attatacgga agtataggag gattaattgc tattcctatt
     4741 ggtgtagttg ttttaaattt aatgcttcat gggttaggaa attctctcga acaaggaata
     4801 tcaattccag tagtaatatc acccattgga gtgattatat ctgttatagt agcaataatc
     4861 gtttcttcat ttagtgcata tatacctgtt aaaaaagcta gccatttgcc gataaaaaat
     4921 attgtactag gaacagtaga agaaaagaat gtttcaaatc gttctatact ttttatagga
     4981 agtataatgt ttattttatc tattttactt ccaagaatat cccctgaaaa tacattgtat
     5041 ctagctggtg gattttcatt actaggatta attgtagcta ctattgtgtt gattcctctt
     5101 attactgata tcatgtctat agtttttgag tttgtttata aaaatatttt agggaatgag
     5161 ggtaaattag ctgctagaaa tatgaaaaat aacaaaaata taatacagaa tatcacttta
     5221 ctatttataa gtatttctgc agttattgca ataagtgttg ttggtaactt tgtcaaaact
     5281 tatataacag atgtgtttcg tgacgcagaa ttacaaggtt ttgcagatgg aaatatgaat
     5341 gaagagttta ttgaagatgt tagacatatg gaaggaatta aaaaaatatt acctctttat
     5401 gttatgaata atgagatttc tggtaatggt gtaacattat caagactaga gggtacagat
     5461 aatataaaac tatataactc tatgtttgga atcaattata caaattttga gataaaaaaa
     5521 caagtaatag aagcttttaa tgataaacgt tctgttattt tgaatgaaga tactctaaaa
     5581 aaagtaggct tatctatagg agatacaata actctatcta atgataagta tgatttttct
     5641 tataaaatag taggtagttt taaatcccgt gcaaatgatg tagaagctgt tataccatca
     5701 cattatgcag ttaatgattt tgataaaact aattatggtt ttttagtata tacagcagtc
     5761 aatccagatg ctgttatgat tcagataaga tatttatttg gagatacata taattggagt
     5821 cgtacagttg aagaatttaa taatgattct ttaaatacta taagtagttt tttgtctcct
     5881 atgaataaga tgacctattt catttttctt cttgctacag taggtattat taataatcta
     5941 ctgattaatt atattcaaaa aagacgttct atagctatgt ataaatctat aggactaagt
     6001 aataaacaaa atataaaagt aacattgatt gaaggggtta cttcaggttt acttggagct
     6061 gttattggta ttgtgatatc tatacttgag attcaaacta tctttatagt tgcaggacca
     6121 aaaatttcaa tgaaaccaga tttagatttt aaaacattta ttattgttgg gttattggga
     6181 attatagtaa ctttaatagg ttcgatagtg cctattataa aagggaagaa aatgaaactg
     6241 atagaagaaa ttaagtttga ataattatgg aggttaatat gaaaattaca cataatagtt
     6301 tagctgtaga agcaaaaaat attatcaaag aatataaaat tggaaataca acaactcgag
     6361 tgttaaaaga ggtatcttta caagtaatga aaggtgaatt tgtttctatc atgggtcaat
     6421 ctggttctgg taaaagtact cttttatata ttttaggtgg tttagatact ccaacgagcg
     6481 gaaaagtgta tatgaatgga gctgatatat cacattttaa tgatgagaaa atgagcataa
     6541 tacgtcgtag aaatatagga ttcgtatttc aattttataa cttaatacca aatttaaatg
     6601 tggaggaaaa tataatgctt cctttgttat tagatggtaa aaatttaaaa gattataaaa
     6661 atcaattaga tgaaatctta gatatagttg gtttaactga tagacgtaaa catacaccac
     6721 gagagttatc tggtggtcag caacaacgtg ttgctatagc tcgagcttta attggaaaac
     6781 cagagattct atttgctgat gagcctactg gaaatttaga tagtaaaaca gggatagaaa
     6841 taatagattt actgaataaa ataaacagag acaatggaca aactataatt atggtaactc
     6901 actcaccaga agcagccaaa agtagtagtc gtactattac agtttcagat ggtttaattg
     6961 tataaggact aattattgta tttgagataa gcatttttaa ttatataaat tagacatatg
     7021 taaagtggat ataagttaaa tactataaat gtgctataat attaaaataa cagataaaag
     7081 tactatattt tttattgatt attttaattt tatttgttta ttaaatatct aatgagtatt
     7141 catactatct ctattattaa aagcagttaa tgatatatag agataatatg gattcttctt
     7201 actttaataa atattcacga taggacagga tataatatat gttaaaaaaa gaaagtattc
     7261 attttataaa taaaaatagg tcacttatag tttgtgaaga caattttgtt tatctcttgc
     7321 cttgtataca attaagaaat tggatatcga attttactat atcgtttcca aatcaaacaa
     7381 taatttctga taattacacg attattcctc atggaagtgt aactttggtg tttttttatg
     7441 atgccacagg gttacatagt ctattatttg gtccaactac taaaccaaaa acagtaggaa
     7501 atattgctaa tagatgtgat gttatactaa ttatagaatt tcaacctgct ggttttttcc
     7561 cgcttatagg tatacagcaa agtgaactta ttgataaagt tgttccattt tctataatta
     7621 atacttcatt agatttagaa attaaaaaaa tatttaatga atctttatct atagataaat
     7681 tgattttaaa gttggaagaa ttattgattt ctaatattaa aattgaatac tcatatgagt
     7741 ttattttggc aattcagttg attattcaaa actctggaaa tatatcttca caagaaattt
     7801 ctaaaaaagt attttatagt tctaggcatt taaacagact ctttaatcag tatctagggt
     7861 tgagtatgaa gtcattttct cgtttggtac gtattaataa atctataaaa cttctaaaca
     7921 ataataaaac ttcatggatg tctatatgta atgaattagg attctatgat ataccacatt
     7981 ttattaaaga ttttaaaatt gtctgtggaa taactcctca aaaataccga gctaatatgt
     8041 ctgattttta cagtgaaatt gcaaaatttt aagttataat acttataaca aagaaaggat
     8101 agtgataaga tgaaatttgg aggagtattg ctaattgtca aagacataaa taaatcaaaa
     8161 aagttttttg aagatgttat ggagcaaaaa atcacattag atttaggaga acatgtatca
     8221 tttgaaaatg gatttggatt acaaagtaat tataaagcta taattggata tgatttacca
     8281 atatatcaac aatcaaataa ctttcagttg tattttgagg ttaatgacat caaacaatgg
     8341 gaaagtaaga taaatagaat aggaaatatt gaatttttgc attatataaa agaataccca
     8401 tggggtcaaa gaacttttag gttttatgat tttgataaaa atattataga aatttcagaa
     8461 agtatggaaa gcgtcataaa gaggctttta aaacaaggtt tagctttaga agaaatttca
     8521 aaacgcacta tgtatccagt tgaatttatt acacaatttc aacaagtata aaatattaaa
     8581 atatataatt ttttctttat attgcaaaaa acaaaggctt ggggcataat agaaccagaa
     8641 aaacctgaat ctcaacagca aatggctcat atggatttcg ctgttaagga tttagaagaa
     8701 gcagttcaat atgcaattca ttgtggagct acaatcgcag aggagcaatt tactgatgac
     8761 tggagagtta tgattgaccc agctggacac cctttttgct tatgtcagat gaaatcaatt
     8821 atggaaagta gccatttttc attgttatag accaaaatat tgtttattaa ttacaaaaaa
     8881 gcacaacagt atataccgtt gtgcttttac ttattataca tgaatacact aaaaaatata
     8941 aatagtagag ctttatcaat aatgataatt tttaaaaaat agtatttttt tattgaataa
     9001 tttcttagat attttaaagt atataaattt tgcatatatg aattggacat tatcaaatgt
     9061 gttataatat taaaacaaca aataaaatta ttatattttt tagaataata attttatttg
     9121 tttactaagt ttatgatagg gtatttattt aaggtaataa aatattatat aaaatatttt
     9181 aaagccttag gtaatattag gtagaagttt tagtaaaata tgcaaaaact aattgattat
     9241 gtaccttgta tgggtatata atagatattg ttttaataaa atatttgaaa gggaatgatt
     9301 tgatgaaaat tactttacca gaaacagcta ttgatacttt aaaaagtatc ttaaaagaca
     9361 atcaagataa acctaataat ataagagtat actttgcagg tgttggttgt ggaggtcctt
     9421 cttttggatt agctttagat gagaaaaaag aagatgactt aacttatgaa gttggagaat
     9481 tacaatttgt aatgagctct gatgaatata gtcagtatgg agatataata atagaagata
     9541 caggatttgg atttagagtt attcctgaaa atatgaaaga tcaaggtgga ggaggatgct
     9601 ctggttgttc agggtgccac taagattaag tttttagtaa gtttagaact ctctttatag
     9661 aaataaattt ctattttaag agagttcttt ttattttttg gtgagataat gtataataaa
     9721 aatgtatttt actaattttt tatatgtaag gagaattatt gtgagaagaa gacgaaaaaa
     9781 aggtgcagat gaaaagcttt taagttatac aaaatatgtt ttaagagacg atatagacaa
     9841 attaaaaggc aagtggaatt taaagtttag aaatgataat ccaatacatg ttgaatttgg
     9901 gactggaaaa ggtaagttta taacaacttt agctaagcag aaccctgaca taaattatat
     9961 tgccatggaa cttaaggaag aagttctttt aaaagctgtt gaaaaagcag atgcttctaa
    10021 tttgaataat atactatttt tatggggaga tgtaagcaat attttggatt actttgaagc
    10081 taaagaatta tcgagaattt atataaattt ctgtgaccca tggcctaaaa atagatggag
    10141 taaaagaaga ctcactcatt ctggattttt agaaatgtac aatagggttt tggaagatga
    10201 tggcgaaatt cactttaaaa cagataatga aaaattattc gagtttagtt taaatgaaat
    10261 tgctgcaaat aattggctat taaagaatat ttctcttgat ttaggaaata gtgagtatga
    10321 aaataatgtt acaacagagt atgaagacaa atttatgtca caaggtatgc gaatatttag
    10381 atgtgaagct aaaaaacgta actagtaata tgtttataag tttaatagta gaaatttatt
    10441 ttaatttata ttaataaagt gtacgaaagt aggtatatat aacaaggaaa cagaaccctc
    10501 tcatagaaat taaattatta taataaaaaa tttatgtggg agggtactat gaatttagct
    10561 aatttaatac catttattat ttctgcagca gctttaagtc caataaattc aaatgataag
    10621 gttttgagcg aaaatcaact taaagatatt tttcctgatg caaatttaag agcagttgta
    10681 aaaagatata taaatccaga tgagatgact ataagcaata taaaggcttt agatggagaa
    10741 ttttatgcaa ctggtgagag tatttcaaat ttaaaaggta tatcatatct tgaaaatgta
    10801 gacaatttta ttttttggaa taataatata aaagaggttc ctaaggaagc tttaagtcta
    10861 aaagatatgg attcaataaa tcttgccaat aattatttga ttgatgacga tgtagtaaat
    10921 tctctttctc ataatggtgt ggatgtaaat tgcgatttaa attttattga tacaaaagat
    10981 aatcagtata aactggactc taaatatcat aatgttgata tagcaaaagg tgaaagtatt
    11041 gatttgagaa aaataattac gaagaaaatt gatagttatt ataaatactg ggaagttact
    11101 gataaccttc caaaagattt aaattttatt gtatctgtaa gtgataagag tgtactttca
    11161 tgtgaagata tgataattaa aggtaataaa acaggtaaag cagtggtaaa agttatacta
    11221 agcgacaaaa atgatttaaa tacatctcaa gaagttctaa ttactgtcaa tgtaaaataa
    11281 tctgtcaact tattcaatca ttctattttc aatttaagtg tataatcaaa ataaaggttg
    11341 ttagtaacag aacgctaata ttagtgattt gttactagca acctttaaat tttatagaaa
    11401 tagattatct aaaaaattcc atttcttctg ttagtagccc atattcctat taaaattatc
    11461 agtgaatata ttatatacaa aactattctt atatattgga catctaatcc aaaaaattca
    11521 atgttactct gtgaggtatg caacatcaca aggattgcag atataccaat aagcaaagga
    11581 ttgattacta tttgcttatc agttcttaaa atcattattt tacccatcca aattattgat
    11641 aatataaaaa ataaaatttt aagaccaaat tctaatgtat ctaccaaagc gaaaacatcc
    11701 cttcatttat aataaatttt tcagctcaac aaaatataaa accctacaaa caacttttga
    11761 atccataata agataacttg attaagcact atgaaataag tactataaaa cttcatctac
    11821 attaatatat actacactat acttcaaatt tcttaaacat aaattaatga aattaacaaa
    11881 attttattca gaaacatata aagatatatt ttactattta aatgcatatc attatatata
    11941 cagttttagg aggtgaggta tatggaatct aaatttttta attggtacac tcaatcctta
    12001 gggggaactt ttggcattat atcatgtata tgcgcatatt taaatggata catgtttgtt
    12061 tatacaaata gtgctagcaa atattttgaa agtataggat ttttgggaat aatttcaagt
    12121 tatacactac ttccactttg tatgcttact ttgatttttg gagttatata ttcttatact
    12181 ccagaaacat ccgacaaaag tatttttaat ataccttttt ttactttaaa taaaagatta
    12241 atacttatta cagtagtttt tggttttata ggtgctagag tttactttat aattcctgca
    12301 attctaataa cttttaacct atatagcaat gttttgatag ctaaattaaa gtacatccta
    12361 tgtaaaaata aagaaaatga tactaatttg aaagaaagcg aagtctaaat tgcataatat
    12421 tggtatttgt cttaaaaatt cccgaggta
//
LOCUS       DAELTA010000106        12199 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.106, whole genome shotgun sequence.
ACCESSION   DAELTA010000106 DAELTA010000000
VERSION     DAELTA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.106"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            151..1731
                     /locus_tag="KPG13_002551"
     CDS             151..1731
                     /locus_tag="KPG13_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG0986256.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1905..2351
                     /locus_tag="KPG13_002552"
     CDS             1905..2351
                     /locus_tag="KPG13_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG0986257.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2372..3415
                     /locus_tag="KPG13_002553"
     CDS             2372..3415
                     /locus_tag="KPG13_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG0986258.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            4186..5346
                     /locus_tag="KPG13_002554"
     CDS             4186..5346
                     /locus_tag="KPG13_002554"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG0986259.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            5348..6478
                     /gene="glgD"
                     /locus_tag="KPG13_002555"
     CDS             5348..6478
                     /gene="glgD"
                     /locus_tag="KPG13_002555"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG0986260.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            6507..7949
                     /gene="glgA"
                     /locus_tag="KPG13_002556"
     CDS             6507..7949
                     /gene="glgA"
                     /locus_tag="KPG13_002556"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG0986261.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            7963..10383
                     /locus_tag="KPG13_002557"
     CDS             7963..10383
                     /locus_tag="KPG13_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG0986262.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            10388..>12199
                     /locus_tag="KPG13_002558"
     CDS             10388..>12199
                     /locus_tag="KPG13_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG0986263.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENN"
BASE COUNT         4910 a         1164 c         2259 g         3866 t
ORIGIN      
        1 ttttaattca aaattttttg ttattttaaa ttatttcaaa actcttagtt ttagtgattt
       61 tatttatgat atttatggga ttaaaaagat ttcaatagat atttttggta agtatttaag
      121 aagtctttaa ctttatagga gtgataattt atgctaatag gtacatctaa attaagcaga
      181 aatgttgata ctgcatgtgt agaagaagta gggattccaa tgattgtatt gatggaaaat
      241 gcggttatat ctgcaatgaa aaatatggat atagacatgt ataatagtta tacagttgta
      301 tgtggtgttg gaaataatgg aggagatggt cttggaatag caagacatct aaatacatat
      361 ggaaaagaag taaacgtatt tttagtaggt aatcttgaaa aactaagtga atgctctaaa
      421 ataaattaca atgtactttt aaatatggga attaatatta taaatattaa taaaaatgat
      481 ttaagtgaaa aaaattatgg agaggaaaat gaagtagata aaattattaa agttgagaaa
      541 caaaatatta taaaaaataa aaatttaaca atttttaaag atagagtatc taaaagtgat
      601 gttgtagtag atgctatatt tgggactgga ttaaaacgtg atataactgg tatttttaaa
      661 gaagttattg acatagtaaa cgataatagc aaaaatacgt attctataga tattccttca
      721 ggtatagata gcgataatgg aaatgtatta ggaacttgta ttagagcaaa caaaacgatt
      781 agctttgagt tttataaaag aggttttcta aactatgaca ctagcgcact tataggtgat
      841 gtagttgtag aaaagatagg tgttccagat tttattacaa gaaaatacca tgatgaagag
      901 ttcataactg ataagacttt tgtaaaaaat aatattagaa gaagagataa gcatggtttt
      961 aaaagtgatt ttggaaaagt atctatagtt gcaggttcaa aaggatttta tggtgcaagt
     1021 ttcatagcaa ctgaatctgc tgttaagagt gggagtggac ttgtaacatt aattagtagt
     1081 gaagatgtgc aagaaaaggt atctattaga cttacagaag caatgacttt aaattttgaa
     1141 gaagaaagat taaataaatt acttaattct agtgatgcaa ttggttttgg tccaggtatg
     1201 ggagataata gtcaaacatt tgataaatta cttaagatag tagaaaattc taattgccca
     1261 atagtattag atgcagatgg attgaacgtt atgaaggaca gatgttataa gtttttagaa
     1321 tggaaaaata gatttgtaat tacacctcat ttgggagaaa tggccaggtt gacaggagat
     1381 tcaattgggt atataagaga acatagagta gatgttgcaa aagaatttgc tcaaaaatat
     1441 aatctggtgg tgcttttaaa aggttatcaa actgtcataa cagatggtaa aaaaacatat
     1501 ataaatccta caggaaatag ctgtatggca actggtggaa tgggtgattg tttgcttgga
     1561 atgattactt catttatagg tcaaggtatg gatatattgg aagctacagt tagtggagca
     1621 tatatacatg gctatatagg agataaattg agcaaagcaa tgtatacggt aaatgctacg
     1681 gatttaataa gtaacatttc attaacaatg aaagagttgt tggatatata attggtgata
     1741 tttgatacta ctttgtaaca attttaacaa agtatgttga tataatacta ttaattaatt
     1801 aatttatttt attgttttgt aatttaaatt tatattcaaa aacattgtat aaacatataa
     1861 tttatatagg gttttataat tcatatgaaa agagggtaga tagtatgaag ctaatatggt
     1921 tgatagtagc agtcttattt ggaatagcag aaatgttaac accaagctta acattgatat
     1981 ggtttagtgt aggagctgta atacttatat ttttaagtag ctttatagag agtattttct
     2041 tacaaatttt aatatttgca gtaatttcta tagcaatgtt agtcgtagct actaaaaaaa
     2101 tagtaaaaaa agataaaggc tataaatcta acactaattt acaagcaatg atgtctaaaa
     2161 aaggtattgt tacagaagag atttctccaa ataacacagg tctagttgta gttgaacatg
     2221 aaacatggac ggctatttct atagatggtg aaaagataga aaaaggttct acagtagaag
     2281 tattaaaaat tgaaggggta aaattagttg ttaaaaaagt agacgccaca gttaatgtga
     2341 caaaccaata gaataaagga ggatttatag tatgggtact aaaattgttt taagtattgt
     2401 attaatagta gtagtcgtag caataagttt aacttgtata agagttataa aacaatctaa
     2461 agtaggtata ataatgagac ttggtaagtt tcaaaaagtg gctgaaacag gtgtacattt
     2521 cttaatacca tttttagata aaatggcata tgtaattgac cttagagaaa ttgtaataga
     2581 ttttccacct caaccagtta taactaaaga taatgtaact atgcagattg atacagttgt
     2641 atattataaa gttacagacc cagttagata tgtgtttgag atagctaatc caatagctgc
     2701 tattgaaaat ttaacagcta caacactaag aaatataatt ggtgaacttg atttagatga
     2761 aacattaaca tcaagagata taataaatgt aaaaatgaga actattcttg atgaagcaac
     2821 agataaatgg ggtataaaag taaatagagt agagttaaaa aatataatgc ctcctcagga
     2881 tattcaagtt gcaatggaaa aacaaatgag agcagaaaga gaaagaagag aggcaatact
     2941 tcaagcagaa ggtaataagt cagctgcaat acttcaagct gaaggtgaaa aacaatctgc
     3001 tatattaaca gcagaagcca aaaaagaagc tatggtacgt gtagcagaag gtgaaaaaga
     3061 atctgctata ttagtggcag aaggtgaagc tgaagctata agacagacag ctattgctaa
     3121 ggcacaaggt gaagctgaaa tgataaaaag aactcaaatg gcaacagcag aaggtttaaa
     3181 attagttttt tcagcaatga aagaggctga tatagacaat aatattctag cattaaaatc
     3241 tatggaagct cttgaaaaaa tggctgaagg taagtcaaca aaactggttt taccttcaga
     3301 agcagttaat ttcctaggaa cattcaaagg aataaaggaa gttatgagtg atgataacaa
     3361 agaagtactt gatataaaag aagttttaaa tgataatgaa tcattaaaaa aataaaagag
     3421 ttttgttatc ataggaagga tatggaaatt aaaggtcgaa ttaattaaat atagcacaaa
     3481 aatgcacgtt gaaaagaaga ggacttataa atttttaagt cttcttcttt gtttttatta
     3541 gaaagatgaa attataaaaa taaattaaca gaatttttat acaatttatc ctttactaat
     3601 gtaaaaagtg ttatataata ttatataaaa catatagcaa ataagcatgg ttttagttat
     3661 tctatttata ttattaatat atatttataa aaatataaac attaatacta agaaaactaa
     3721 taaataattt aaaagtttat atccttttgt ttaaattata gacaaagaag tatcaattaa
     3781 aaaataaagt tgattaataa ttacttgatt ttccttggat aattatttaa caaatattag
     3841 attatatttt gctattacga atttaacaaa ttaaaaaata agcatgttca tatgacacaa
     3901 aggatttata aaaaatgttt ttatatttat aaaataaaat ttgtattatt ttttaaccga
     3961 taattgaata ttaataaata ctatagtgct tatggaaaat aatgtatacg tcggtaagtc
     4021 cattatgggg tatacattat atcaattgta tagttgatat tattttattt ttattaaggt
     4081 attatttaag caacatttat acacgacatt tattgactga taaagtttat aaaagtaaaa
     4141 atgcaattat aaatattaat tactagatga ggggagtgtc attaaatgaa aaaagagatg
     4201 ttagctatga ttttggcagg agggcaaggt tcaagattag gtgtttttac aaaaagaatt
     4261 gccaaaccag ctgtatcatt tggaggtaag tatagaatta tagattttgt gttaagcaat
     4321 tgttcaaact ctggtattga tactgtaggt gttttaactc aatatagacc gttgatatta
     4381 aattcacata taggcatggg aagtcattgg gatttggaca gaataaatgg tggtgtctat
     4441 gttttacaac catttatgaa tgaaaaagaa ggaaattggt ataatggtac agcacatgcg
     4501 atatatcaaa atatggactt tgtagatacc tataatccag aatatgtatt gatattatct
     4561 ggtgaccata tatacaagat ggattatagc aaaatgttaa aatttcataa ggaaaaaggc
     4621 tctaaagcta caatagcagt gattgaagtt ccttgggatg aagcttcaag atttgggata
     4681 atgaatacaa atgaagattc aagtatctat gaatttgaag aaaaaccaag tgaacctaag
     4741 agcaatttgg catctatggg agtatacata tttgattgga aaatgttgag aaattatttt
     4801 aaggaagcag aaaaaaatcc agaaataaat tatgatgatt ttggaaaaaa tttaatacct
     4861 aaaatgttag aagataatgt tggaatgtat gcttatccat ttaaaggata ctggagagat
     4921 gtaggaacta tacaaagtct atgggatgct aatatggata taataaaatc tccagaaaca
     4981 cttgatttag cagaccctaa gtggaagata tatacaaata ctatggcaat gcctccacaa
     5041 tatataggta aaaatgcaaa tgttcataga tcaatgatag ctgatggttg tagaatttta
     5101 ggtgaagtag gtaactctgt attatcacat ggagttgttg ttggtaaagg aagtaaagta
     5161 attgattcag taataatgcc aaatgttgta ataggtgaaa atgtaactat agaaaaagct
     5221 atgataggtg agtgtgcaac tataaatgat aatgttcaga taaagaatgt aaataatgaa
     5281 ataaatgtag tttctgagta tgaaaatatc gaacctagat gcgtattaat agaaggaggt
     5341 ctttaagatg agaaatgaat gtttagggat aataaattta aacaaaaaag gagacccagc
     5401 aataaacaaa cttaactatg gaagaccaat agcatctaca ccaatagctg gaagatatag
     5461 aataatagac tttgctttat ctaatatgat aaattcagga atcactaaag ttggaatatt
     5521 cgcaaaagaa aaatacagat cattaacaga ccatataggt agtggtaaag attgggattt
     5581 aagtagaaaa aaaggtggcc tatcaatttt tagtccagaa aacactaagt atagaaatac
     5641 ttactcacat agagaaggtg atatatatac tatcttagct aatctagatt atatagaaaa
     5701 gagtgaagaa gaatatattt taatagctcc aagctatatg ctatgcaatt taaattattc
     5761 tcaagcattg gaataccata aaaaatctca taatgatata actataatat ataaaaatgt
     5821 aaataatgca aataaagatt ttgctggtaa cttaactcta aatttagata gtaacaatag
     5881 ggtaataaat gtaggaaata atttaggtaa gttccctaga gcaaacatat gtatggagac
     5941 atatattatg aagcgtgaag attttgttga atgtatatat aacattgtaa ataaaggcaa
     6001 ttattgttat ttagaagaat ttattattga agaagcagaa aacatgaaaa taggagccta
     6061 tgaatataca ggatatctaa aatgtgtaaa ttctgttgaa tcatattttg aaatgagcaa
     6121 agatttatta aatttagaag ttgcagatga attattatat tctgaaagaa agatatttac
     6181 aaaagaaaag aatgaatcac ctacaattta tacagatagt gctaaagtag aaaatagctt
     6241 tatagcatct ggttgtttaa tagaaggtac tgtaaaagat agtatcatat ttagaaaggt
     6301 aaatgttgaa aaaggaacag ttatagagaa ctcaatagta atgcaaaatt gtgtaataaa
     6361 gtcaaatgca aaactatata atgcaatatt agataaaaat acaagtgtat caaatgggaa
     6421 agaattaaga ggagatgaaa aatatcctct tgtagtggaa aagaatacaa atatataaat
     6481 aaatttttta gaagggggaa tgactaatga aggtttttta tgtaacagca gagtgttggc
     6541 cctttgcaaa aactggagga ttaggagatg tttcttatgc attgcctaaa gaattaaaaa
     6601 aagaaggtgt agatgtaagg gtaataatgc ccaaatattc aactatacca agttatttaa
     6661 aagatcaact aaaagaaata gctgtgttta gtgtacgtgt tggatggaga aatcaatact
     6721 gtggtctttt agaaatggaa ttggatggtg taaaatttta ctttatagat aatgaatttt
     6781 attttagaag agaagatgaa agaaagagta tctatggata tggagatgat gcagaaagat
     6841 atactttctt tacagatgca gttttagaag ctataagtag aattgatttt tatccagatg
     6901 taatacatat aaatgattgg catacaggga tgttaccatt aattctaaaa gaaagatatg
     6961 caacccttga ggggtataaa aatataaaaa ctatgtatac tatacataat ctccaatatc
     7021 aaggtgtatt tgataagcat gttttatatg atatattgga cttaccacaa aaatactttg
     7081 ataatggaga tatagagtat tatggctcaa taaattttat gaaagctggt ataaattttg
     7141 cagataaaat tataactgtt agtcctactt atgcaaatga aatacaaaca tctttttatg
     7201 gcgaacaatt agatggatta cttagaaaag agtcaggaaa attaaaaggt atattaaatg
     7261 gtatagatta tgatttaaat gaccctgcaa aagataaaga tatatttgtt cattatgatg
     7321 tagactcaat tgacaaaaaa gtagaaaata aattgagatt acaagatata ttgggactaa
     7381 aaaaagattc atctattccg ttgataggta tagtttcgag attagtttct caaaaaggat
     7441 ttgatttaat agcatacatg atgccagaac ttatgagaga agatttacag attgtcgttt
     7501 taggaactgg agaacatcaa tatcaatcaa tgttcaacta ttacgattct aatttttcag
     7561 ataaagtatc agctagaatt acttttaatg catcattggc tcaacagata tatgctgcaa
     7621 gtgatatgtt cttaatgcca tcattatttg agccttgtgg tataggtcaa atgttagcta
     7681 tgagatatgg ttctttacca atagttagag agactggagg gcttagagat acagtaacac
     7741 cttataataa atttactgga gaaggcaatg gatttagttt taaaaattac aatgctcatg
     7801 agatgttttt ctgtttgaaa aatgcaatca aagtatttaa ggacaaagaa aaatggatta
     7861 aattagttga aaatgcaatg aaaacagata atagttggaa aaaatcagct aaggagtata
     7921 tagaaacata tagagacata tgtgattaaa ggagtggttt ttttggtaac aataagcaaa
     7981 aagaaattca agcaaaaatt tgaagtaaaa atgtatagct tatatgcaca atctatcaag
     8041 gaagctactg atgaacaatt gttaaatgta ttatgtagtc tattgaaaga tgagatagca
     8101 aaaaaatggg ttgctacaaa gttagataag aaaaaagaag tttattattt tagtttagaa
     8161 tttttaatag ggagacagtt aaaatctaat ttattaaatt taaatattga agaagaagtt
     8221 agagaaggtt tatctgaatt aggaatcaac ttggatgatt taattgaagc tgaagtagac
     8281 ccagccttag gaaatggtgg acttggtaga ttagcagctt gctttttaga ttctatggca
     8341 tctttgaaca taagtggtca aggatatgga ataaggtata aatatggtct gtttgaacaa
     8401 aaatttgtaa atggttatca ggtagaagtg cctgataact ggcttacaga aggacgatat
     8461 gcatgggaaa ctgtaagacc aaatgaagca acaatggtga agtttggtgg tgaggtagaa
     8521 ttaatcaagg aaggtagtca tttaaaagta attcataaaa actaccttcc tgtaatggca
     8581 atgccttatg atataccaat aatcggatac caaaaccaat gtataaatac tttaagattg
     8641 tttaagagtg aaataccaaa gagagatttt ggtgaactta cctcaaatgc tctaaattat
     8701 tctggtagct atgaagaggc tttaaaacat aagtattata ctgaggaaat atcacaggta
     8761 ttatatcctg atgattcaaa ctatgcaggt aagttgttga ggttgaaaca agaatacttt
     8821 tttgtaagtg cagggataca agatataata agaaaatata aaaagaacaa attaaatatt
     8881 aataatttat tcgataaggt agcaattcat ataaatgata cacatcctac cttatgcata
     8941 ccagaactta tgagagtttt gcttgatgaa gaaaatttat cttgggatga agcatgggaa
     9001 ataactaaaa aaacagtgtc ctatactaat catactataa tgtcagaagc aatggaaaaa
     9061 tggcctgtaa gtatgatgaa agaactatta ccaagaattt atatgataat agaagaaata
     9121 aatagaagat atgtagagga attaaataat aaaggatatg accaagatag aataaaaaga
     9181 atgagtataa tagactgtga caatatcaat atggcaaact tatgtattgt cacaagtcat
     9241 agtgtaaatg gagttgctaa gcttcatact catattcttg aaacagaggt attaaaagat
     9301 ttctatcaag atgaaccaaa taaatttaat aataagacta atggaattgc acataggcga
     9361 tggcttataa gttcaaatcc tcaacttagc aatttaatta cagatttaat tggtgattca
     9421 tggaagaaag atacattaca attaaaaaat attgaaaaat ttaaaaatga ttcctctgtt
     9481 ttacaaagac ttgatgatat aaaatacaat aataaagcaa atctagcaaa gtttataaaa
     9541 gataaatacg atttaaatgt agacccaagt tcgatatttg atgttcaagt taagagattg
     9601 catgcgtata aaagacagtt actgaatata tttaatgtac ttcatatgta tcatgaatta
     9661 cttgataatc ctaatttgaa tcttgaccca agaactttta tatttggcgc aaaagcagca
     9721 ccaggatatt atttggctaa atgtataatt aaatttataa attctgttgc aagtactata
     9781 aataatgatg ttagagtaaa ggacaagtta aaagtagtat ttttagagaa ttatggagta
     9841 tcattagcag aaataataat accagcagca aatgttagtg agcaaatttc aactacaact
     9901 aaggaagcat ctggaacaag taatatgaag tttatgatga atggtgctat aactttggct
     9961 acacttgacg gtgcaaatgt agagatatgt gaacaggttg gtaaagaaaa tatgttttta
    10021 tttggactta gtgcagaaca agtactaaat tataataaat atggtggata ttcttcactt
    10081 gacctatatc attctaatat ggatataaaa agggtagtag atgatttaat aaatggattt
    10141 attcctaatc ttggcgaaga aggaagaagt atttataatt cactcactac ttacaatgat
    10201 gagtattttg tattgagaga ttttgaaaat tatggtcaag ctcaagcaga tataaacagg
    10261 ttatatagag ataaagaaaa atggaataaa atgtctttag ttaatatagc taattctgga
    10321 tttttttctt ctgatagaac aatttctgag tatgcaaaag acatttggtt taaaagggtg
    10381 tgattaaatg caaagtgtaa tagaatataa ctcttgggat aaaaatttta aggctccgtt
    10441 tggagcttta aaatttgatg aggaattgac catttgtgtt aaagtaaatg agggatacaa
    10501 tatcaagtct atttccctag aaataaatag agaagaagaa atgagaacaa taactttaaa
    10561 tgaagaatta gataatgata aacttggaaa gtgcttttgt ggtaagattg aaaagtttga
    10621 tggtacaggt gtttattttt attactttaa agttgatgtt gaaatggatg gtcagataaa
    10681 gactttattt tatggaaaaa acagagataa tggatattct tgtgaatata attatagtga
    10741 tattaataaa tatcaaatca ctgtttacaa agattttaaa gtaccaacat ggtacaaaga
    10801 aggaatactt tatcatatat ttgtagatag attcaataat ggaaatcgta gtggtaaggt
    10861 tgataatcct aaaaaaaata gtttcatata tggaaattgg gaagatacac ctatgtacat
    10921 aaaggatagt caaggggatg ttataagatg ggatttccat ggtggaaatt taagagggat
    10981 aatcaataaa cttggatatt taaaaaaatt aggtgtaagt atactatatc taagccctat
    11041 atttgaagcc tctagtaatc ataagtatga tacaggtgac tataaaaaaa ttgacccaat
    11101 gtttggggat gaagatacat ttaaagaact tattgataaa gcaaaagaaa aaggtatatc
    11161 aatagtatta gatggagttt ttagtcatac tggagcagat agtaaatatt ttaatatgta
    11221 tggaaactat aatagtttag gggcatatca atctaaagaa tctccatatt attcatggta
    11281 tatgtttgaa gaatttcctc aaaaatataa aagttggtgg gatgttaaga cattgccaaa
    11341 tataaatgaa ttagaacata gctatatgga ttatataata tatgacaatg atagcgttat
    11401 aaataaatgg gtaaatatgg gtattaaggg ctggagacta gatgttgcag acgaattacc
    11461 aactaagttt ataagagagt taaaaaagga attaaaaaag gcagatgatg actcaattct
    11521 tataggtgaa gtatgggaag atgcatcaaa taagattagc tatggtcaga gaagaagtta
    11581 tttattaggt gaagaattag attctgttat gggttatcct tttagaaata acatgttttc
    11641 tttcttaaaa ggagaaatta attcatatga actttgtaat agatatatgc agattaaaga
    11701 aaattatcca aaagaatcat ttaggtctaa tttaaattta attggaacac atgatgttac
    11761 aagagctaaa acggaattaa atgatgatgt agatttagtg aagcttgctg tagctataca
    11821 gatgacattt gaaggagtac catatatata ttatggagat gaagcaggtc tttgtggagg
    11881 tgttgaccca gataatagaa gaacttatcc atggaaaaat gaagacgaag atatgcttaa
    11941 tttttataga gatgttataa gaattagaaa taagaataaa cttttaagtt ctggaaatac
    12001 agaatttata tatactaata atgattctgt attttctttt attagagtta atgaaaataa
    12061 tgatagaatg ttaattttag taaatagaag tgaaaatgtg gaaagtatat cattatgtat
    12121 agagagtagt tttattgagg aaatacctat aaagtatagt cttaaaaatg caaatagtac
    12181 tatacaaatt gaaaataat
//
LOCUS       DAELTA010000107        12058 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.107, whole genome shotgun sequence.
ACCESSION   DAELTA010000107 DAELTA010000000
VERSION     DAELTA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.107"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            183..701
                     /locus_tag="KPG13_002559"
                     /pseudo
     CDS             183..701
                     /locus_tag="KPG13_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1133..1387
                     /locus_tag="KPG13_002560"
     CDS             1133..1387
                     /locus_tag="KPG13_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986264.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1423..1746
                     /locus_tag="KPG13_002561"
     CDS             1423..1746
                     /locus_tag="KPG13_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986265.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            2020..2172
                     /locus_tag="KPG13_002562"
     CDS             2020..2172
                     /locus_tag="KPG13_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986266.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2366..3289
                     /locus_tag="KPG13_002563"
     CDS             2366..3289
                     /locus_tag="KPG13_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG0986267.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3556..4305
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3556..3584
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4496..4648
                     /locus_tag="KPG13_002564"
     CDS             4496..4648
                     /locus_tag="KPG13_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986268.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4768..5581
                     /locus_tag="KPG13_002565"
                     /pseudo
     CDS             4768..5581
                     /locus_tag="KPG13_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5633..5794
                     /locus_tag="KPG13_002566"
     CDS             5633..5794
                     /locus_tag="KPG13_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986269.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6122..6586)
                     /locus_tag="KPG13_002567"
     CDS             complement(6122..6586)
                     /locus_tag="KPG13_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986270.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6722..7039)
                     /locus_tag="KPG13_002568"
     CDS             complement(6722..7039)
                     /locus_tag="KPG13_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986271.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7322..7618)
                     /locus_tag="KPG13_002569"
     CDS             complement(7322..7618)
                     /locus_tag="KPG13_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986272.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7637..8050)
                     /locus_tag="KPG13_002570"
     CDS             complement(7637..8050)
                     /locus_tag="KPG13_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986273.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8034..8267)
                     /locus_tag="KPG13_002571"
     CDS             complement(8034..8267)
                     /locus_tag="KPG13_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986274.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8486..8860
                     /locus_tag="KPG13_002572"
     CDS             8486..8860
                     /locus_tag="KPG13_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0986275.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            9472..10164
                     /locus_tag="KPG13_002573"
     CDS             9472..10164
                     /locus_tag="KPG13_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986276.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10360..>12058
                     /locus_tag="KPG13_002574"
     CDS             10360..>12058
                     /locus_tag="KPG13_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986277.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     "
BASE COUNT         4924 a         1209 c         1917 g         4008 t
ORIGIN      
        1 tttgtatttt atttatataa attttaatag aatatgtata gtatatagta gatagaatgt
       61 ataaaaataa aaactgttca ataagaatta atatacatta attcagctga atccaatcaa
      121 aatacttaaa taaaagtaat ctgaaaataa cataattcaa atcaatttat tcaaataaac
      181 ctcaatttat ctcaaatctt aagaagtcat taaacaaact tgctaaatta caaagagaat
      241 tatcaagaaa aacaatcggt agtttaaata gaaataaagc aagattaaaa gttgcaagac
      301 ttcaagaaca tatagctaat caaagaaatg attttctaca aaaactatct actaaattaa
      361 taaaagagaa cgatattatt tgcatagaag atttacaagt aaagaatatg attaggaatc
      421 gtaaactatc tcgctcaatt tctgatgtgt catggtcaga gtttattcgt caattagaat
      481 acaaggctaa ttggcatggt agacaaattg taaaggtggg taaattcttt gcaagttcac
      541 aaatatgcaa taaatgtggg tataaaaacg aggaagtgaa ggacttaaat ataagagaat
      601 ggatttatcc tagttgtaat gaaactcacg atagagatat aaatgcaagt ataaatatat
      661 taaaagaagg attaagacta ataacaattc aaaataaata acgtatgtaa gaaccgtagg
      721 aactacgggg atagcctgtg gagaattagt aagacatatg ttagtatgca aaattctgtg
      781 aagcaggaaa cctgtgactt tagtcatggg aggttcaggc tagtattact cattattgta
      841 ttcaaaagct aaagtggaaa cctagtgaat atatgaattt agaagttaat gagagagcat
      901 tagcagccga ctcaatactt attaaaattg aagatgaaga agaaacaatg aaagaagctg
      961 aaagagagaa aaagagaggt agaagaaggt agaatttaaa gttaaaatag ttacattgct
     1021 aatatatgaa aatgttacaa atttggaaat agatggatat atatgttgta tagtataatg
     1081 tagataatat atcaccaagt aaaaaacaac aaataaatct aaattatgag gggtggaaaa
     1141 taaagatatt aaacttacaa tatgtatatt tgcttttgta attggaataa tagtgttatt
     1201 ttgctgtata ggtctaggtc aatttaatat ggattgtatt ataaaacaaa atggtgggtc
     1261 aatagctacc gaacaataca acatatattt agaacaatct atttcacaat atagaaattt
     1321 tggttctata ctggttttac taggaggaat aggaatactg tttgataaaa gtgtcagaaa
     1381 atactaaaaa agtatatttt tatttaggat gtgatgaatg atatggcaag agatgtattt
     1441 agtgtaaaga tgacaccact agaagcacag aaaatggtag aaaaaaattt ggaagcagat
     1501 ttagtttctg ttgatattta taacctagaa aataataagt ctataatagt tacaatcttt
     1561 gaaaaatatt atgcaagaag caatagtgat gcaggtttgg tagtgatttg tgaaaatact
     1621 acaggaaaaa cacttgttaa aattacctca actggtagtg caattggact atttcagatt
     1681 gattggggaa caggtaataa tttgattaaa agagtaaaaa aaatacttat tgattatatt
     1741 atttaattat tttatgtaga tattcttagg ttggtatgga atttgtacta acctattaag
     1801 taatcaataa aactaaataa acatagaaag cacttacaaa tatgtaggtg ctttttattg
     1861 aaaaaataga aaataaaata aaaagtaaga tataggaaaa atatgtaaga attatatgtt
     1921 ataataattg tagcaagaag atgtaatcta caatttatag agtggagttc atgtattaaa
     1981 aaattatcct cccaacgata agaagggagg tgaatataca tggataattt tttatttaat
     2041 gtgttagcta gtgtaacagc tagttacata gtttatttga ttagcaagtt aatcaaaaaa
     2101 gtaaaaagcc actctaccgc aaagagtgac ttaaagattg atataaattt taaatatcat
     2161 cgtaaaaaat aactttcagt tatagaactt cactctaccg caaatagatt gtagttcttc
     2221 ttgtttttat tataccacaa attagaaaaa atattgcata taatattttt tatagtcaat
     2281 aaaaatatga aattttttat acacaatgat gatgaatcta actagatgaa taaatttaat
     2341 tgtaaataag aaagtactta cttaaatggt aggtgctttt gttttgccaa attatgctat
     2401 aatattgagt gagatgagta atttgtgggg gaattttata atgtggatat tagtaccaat
     2461 aataacaata gtattactta taatagctgt ttcttctatg caatatattt tagttatgat
     2521 agcttttttg ttgattatat attcttttat agaaaagaaa atagttatgg gtcttgtgtc
     2581 agttcttttt ttcacttatt caatttatct ttgtgcaact tgggaagata aatccttgat
     2641 agcagataat aaagttgaga ctgttagggc acaaagagaa actgtagaaa gagaaaaaga
     2701 aatggaaaga agaagaatac aagaagaagt agacaaagaa agatatattg aaaaacatgg
     2761 aatggaaata tcagaaaatg atttaaaagt taagctagag gctttagtac ctcaagaata
     2821 taaggggaag aaatatgaat taaaagtagg aaaatttaaa agatatagta tgtattttga
     2881 tttaactgta caaaatgaaa aatttagtaa ttctgaagaa tgtaaaaaat ttgtaaaaga
     2941 aattgctaat gatcttaaaa aaattaaaat aagcaaggcg tactttaagt ttcattcaaa
     3001 agatgacggc ggtatatata attctgttta tattgattat tttagaaaca tacaaaataa
     3061 tgtagataat gttgaaaatt tagaattcaa tgagtttgaa cttaaaaccg aagaagaaga
     3121 gaagcgagaa caagagaaaa tagaacaaga aaaaaacagt tataataact acattcaaaa
     3181 cagagtggtt gacccactag atagaataaa gaaactaaaa gagttgctag attcaggagc
     3241 aattacacag gaagaatata ataaaaagaa aaaagaatta ttagaataga taatatagta
     3301 agcacttaca agtatgtagg tgcttttaaa tttacaaagt attccatttt aattttatag
     3361 tttagatttt atgatataat aaaaatatag aagttttgca gtgtgcgata tttgttacaa
     3421 agtagggctt aatacttgaa atctaagatg ttgagggtgc gtgataagtg ttatcaattg
     3481 cactattgcc cgctcactgc aattttaaga gtattgtata tatgtaggta ttggaaatgc
     3541 taagtttatt ttggggtttt agattaacta tatggaatgt aaatattaca tgggtaaaaa
     3601 ttttgatgat ttttatgaca gttttagatt aactatatgg aatgtaaatt gtatagaatt
     3661 aagaattttt ttaatactaa ttatagtttt agattaacta tatggaatgt aaatattaca
     3721 tttaatttgc attgctttga tgacttcttt gttttagatt aactatatgg aatgtaaatg
     3781 cgtacaaggt tgagaagctc cgcaatctca acgtcgtgtt ttagattaac tatatggaat
     3841 gtaaatattt gaaaggaata aattttaaat gaatgaaagg ataggtttta gattaactat
     3901 atggaatgta aatcagccaa aaacttcgaa tttgaactaa aatgcgtggg ttttagatta
     3961 actatatgga atgtaaattc tcatgactat tgaataaata taataaacaa gcatacgttt
     4021 tagattaact atatggaatg taaatttaga atcaaaagca cttaatccta gttctttcat
     4081 agttttagat taactatatg gaatgtaaat gatgaacaat aaaacaatca tctaaagacg
     4141 aagaaagttt tagattaact atatggaatg taaatgttcc tgttgtgttt tttactaatc
     4201 ccattttata tgttttagat taactatatg gaatgtaaat tgctgtgatt tcactaataa
     4261 aacaattaat aattgtgttt tagattaact atgtggattc aaaattaaat aaacaaagaa
     4321 agcacttaca aatatgtagg tgcttttatt ctgctcaaaa ttggtcggtt gggtaaaatc
     4381 attagaaaaa attagtaaaa acctcttttc tgtaactcgt tacaatatta ttattaatgt
     4441 aacgagttac agaaaagagg tgaataaaat agcaactaaa agtagggcag agtatatgaa
     4501 aaattgtcga aaagataaaa gaggttttag tgtactttta gacaaagaaa agttagataa
     4561 atttgatgaa gtattagagg aaaagaatct aaccaagaaa gaatggctag aagaaaaaat
     4621 cgacgaggaa ctggaacaaa aggaataaaa aataagggtc actcccaccg accaaagttt
     4681 gagtaacccc tatgatgtat actatcgtat atcaattata atatatgtca ttccttaaaa
     4741 aaatcaatta ttaaggagtg taatattatg aaaaatgaat taatgatgtt tgaaggaaaa
     4801 gagattgaag tatttgaatt tgaaggaaga attttattca actcaaaaga tgtagcaaat
     4861 tgtttagaca taaaaaatgt taatgagaat ataatactta tgaatgaaaa gcaagttgta
     4921 aagttaagaa attcggatat cagtaatact gacatccgaa aacttaataa tgctggagaa
     4981 aattttctta ctgaaagtgg agtgtataaa ttaatcttta aatcaagaaa agaagaagct
     5041 gagagattcc aagactggat aagcgatgaa gttttaccaa gcataagaca aactggtgca
     5101 tacataacta ataatgtaat cccgaaaagt tgagagaaaa agcaagtgag attgaaaaat
     5161 tacagttgct tataacagta catctatgtt aaaagaattg ctagatgatg caggctttga
     5221 caataaatca aaactattaa cagctaaaac attatataag aaagcagaaa ttgatttacc
     5281 aatagagata aacgaagaag aacattattt tgatacaaaa caaatagcat ctaaactgaa
     5341 aatatattct aagagtaata aaccagctca gatggctgtt tgtgagatta ttaaaaagat
     5401 tgatttagaa gatggtgaag ttaaaggtgt ttgggaaatt aatggttctt ggactggtac
     5461 tgtaaataaa tatacaaaga gtgtaataga taaggtgaga acttggatag aggaaaataa
     5521 tagacctacc aagattgcag gtgagaagaa gaattattat gtattttaca aaattgagta
     5581 aatttatcag ttgtattaaa taatttagtt tagttttggg gggattaata caatgtgtga
     5641 gaatttactt gataatatgt atattgaaaa gagaaaagaa gaatatagaa ttaaactttt
     5701 gaaaataaga gaaacagata tagatatata taataagtta gaaagtatag tatataaact
     5761 ttctgagaaa aaattagaga aaaataataa atagacatat aaagcacttg gatattctac
     5821 tgtttcaagt gcttgtttgg tataaaatgg tataatggag atagagaatt atattaacca
     5881 aaaaatggag aaaatagaga agatgattta gttgacgctt tatcagtagg agtacctgca
     5941 ggtaagacag aatctctagt acgattagtt ggagatggaa taagtgatac tcaaaaaagt
     6001 ttaataaata aaagagaatt tagaaatata actcaaattg gtggaaatgg aaatgagaaa
     6061 gcatttagtg aagtagaaaa cttagttaaa tagattagaa ggtagcaact agagttggtt
     6121 gttaccttct ttttttatac tttctctctc taagtatttt atttttcata tcttctaata
     6181 catctaatgc agtagtataa aattgtatat tatttctata aggtatagct attggaagaa
     6241 tgcccttgtt aactaaaaaa ataagaagta caataaataa aagtgtcata atcattctaa
     6301 taaaataatt agtgatatct gataaatcaa atgttaagtt aattgttgta gaaaatacaa
     6361 cagccataat agatatatac aaagcaaaaa tggggttact atttttttgt aactgtcctt
     6421 taagcctaga ttgctctatt tctatatctt gaatactttt tatggaatcc atttcactat
     6481 aatacttata aacttttaaa taatattttt tatacttgtt taaagcttct tcattatctt
     6541 taaggtcttt gtcacttaac tctgatttaa cttccccaaa atccataaaa tccctcctac
     6601 aatgttatat aatatacttt aatttatagc atatttaaga acttatatca gcaatttgtc
     6661 gaacgatttt agctaaaaat agagtataat gataggctta ttatttataa gatattattt
     6721 tttatatagt tatatatttc tttctaatta gataatataa tgctgttgtt ataatggcta
     6781 ctaatattat tgctattatt gtaatatcta atcttcttgt aaataagcct attgctatat
     6841 actcaagacc tgttacatag cgataaagta cttctagttt aacagttttt ttcttatcta
     6901 tatttccata tattgattct aatttattaa aatatttttt actacaaaaa gcagttttag
     6961 gtttaattaa atatattatt gtagttccta ttataactat tattccaata agtataagat
     7021 tgatatttcc aaaatccata gcagatacct cctattaaat ttgcttttct taatcaaagt
     7081 atatcagtaa cacaagtaca aagagtatct aaaatattac aaatattaac aggatagata
     7141 aaactatagt aaaaattgta ataataaagc gaacgaaaat tatcatacta aaacataaga
     7201 catgttataa ttgtattaga tagaagatta acatacacca aagcactcct tataataaag
     7261 agtgcttatt tttttgaaat tcatcaatat ataaactatc aagaacctta ttcaacacac
     7321 cttaaaatcg atttaaatag ttctttttga taacataatt tatatgatat aataaaaaaa
     7381 tagaagtgta ccaccaatac acttctatag ctataactaa gcattctctt gctgggggag
     7441 tgcttttttc atttctccaa acaacattat ttacttcatc agtaattatc aaatcaatct
     7501 cattatcttt caaaatctct gttgcagtac aaattagatt atctaagtca aaacttcgag
     7561 ttatctctag ttgtttgtta tttacttctc tttttagtat ataagtttta aaatacattt
     7621 atatcacccc ttgtatttac tcttttttct ctaaaaatat attgataagt aaatatgcaa
     7681 caaataaaaa aatctcaatg acacatagtg ttatagctat aactagaagt gattgatttt
     7741 tccatgcact ataagctatg atgaaaagaa atgtaaaagt aaataatgta taattcataa
     7801 ttttaactaa gatatgactt gttttcattg ttaagtacaa gtctctttca tctgtactat
     7861 cgtgtaattc ttcaacaatg ccttttcttg aaaaagcatt acaaaaatta aagattgcta
     7921 ttacaattaa aagaaatgat gaaattagga atcttttatc ttttaaaatg caataaataa
     7981 ctaaactagc acaacataac acacaaacta taatacccac tataaaactt cgtttactct
     8041 ttactttcat gttttttatc ctccatttct ttattttctt ttaaacaaca tagttcttca
     8101 atcgttgtac caaacacttg tgccatacga taggcaagca taagagaagg gctatattgc
     8161 tctttttcaa tagaaataat tgttctgttt gatacatgga ctaaatcagc gagttgttgc
     8221 tgtgttagac ccatatttgt tcgaaattct cgtacttttg ttttcattcc ataacctctt
     8281 tctatatgaa gctaacttca tgtgaagtta acttcataat atcctaaaat gagtaaaatg
     8341 tcaatctcat tagataagta ttataaaacc taaaagaata atatggtaaa aattggatgg
     8401 aattatttac cttaattctc ttgtttgtaa aactttattg atatagaatt taacatatac
     8461 ataaatcggc aaaggggcgc gaattatgaa aattagtaaa ttaccagaag cggaattaaa
     8521 agttatgaga tatatttgtg aagctaacaa ggtattaact tctagggaat tagttgctgc
     8581 aatggaagaa aaatatgagt ggaatgaatc aactacattt acagttttga aaagattaga
     8641 gcgaagagag tttttaagta cagagaaaat aggcaaactt acacattata atatactagt
     8701 aaaagagaaa aaatatctaa ggtttgaaac aaaggaattt ttgaagaaca tacataaaaa
     8761 ttcaatttca agtttaatgt cagcgttaca tggtgaagac gacgaagtgg atgaagataa
     8821 attaagtgaa ctcgaagaat gttttaagaa tttgaaataa gaaaatgaca aattgataca
     8881 taataaataa aatgaaggta tgaaattata actttttcta ccttcatttt gtttattatt
     8941 ctgaacatgt taagaaaatg aaataagttt gtattttagt tatataaatt ttaatagaat
     9001 atgtacagta tatagtagat agaatgtata aaaatagaat ttaaataagt agctattatt
     9061 gcagtgaaat gaatgtttct aagtatattt gggtggaaga atggtggaag aaaattagtg
     9121 aaatgtaatt cttccaccat tcttccacct agaaaataga aactgtttaa taagaattaa
     9181 tatacattaa ttcagctgaa tgcaatcaaa atacttaaat gaaagtaatc tgaaaacaac
     9241 ataattcaaa tcaatttatt caaataaacc tcaatttatc ttaaatctta agaaatattt
     9301 aagaattagg ataatatttt cttaagattt atattttatt atatagttat aagggaaagt
     9361 tacaaaaatg taagccaaag taatataaca atttagtcat tgttgagaaa gggagtgaaa
     9421 gatattgtat aatatagata agtattatta tacaatagag gaagtgaaaa tatgtacaat
     9481 atattagtag tagacgatga taaagaaatt gttgattcga tagaaatata tcttaaatca
     9541 gaaggattta aaatatataa agcatatgat ggaatgaaag ctttggaaat aataacagag
     9601 aatgacattc atctaatact aatggatata atgatgccta aattagatgg aatcaaagca
     9661 actgtaaaaa tacgagaaga aagaaatatt ccaataattt taatttcagc aaaaagtgaa
     9721 gatacagata aaataatagg attaaatatt ggagctgatg attatataac aaagccattt
     9781 aatctattag agcttatagc tagggttaaa tcaaacttaa gacgatatgt aactttaggt
     9841 aattatgaag atacatgtaa agaaattcta aaaagtggag ctttggagtt aaatacacta
     9901 acaaaagaag ttaaaattga tggtgaaaac ataaaaatga ctccaattga gtataaaata
     9961 attgaacttc ttttagaaaa taaaggacga gttttctcaa tagatgagat atacgaaaaa
    10021 gtatggaatg aggaaagttt taattcagaa aatacagtag cagtacatat cagacgaata
    10081 agagaaaaga ttgaaataaa tccaaaagaa ccaagatttt taaaggtggt gtggggagtt
    10141 ggttataaga ttgaaaaatt ttagtggaaa aattataaaa gaaaagagtt taatatttaa
    10201 ttgcattttg attttagtca tttttacatt tacacaaatg ttaggaattt taatatgttt
    10261 taattttttc tctgagagtt taatttactc tatggggatt atgcttttct taaactttat
    10321 tttatataaa atactgacaa tgtagatgag gtgggcaact tggagaaata tagagtatta
    10381 aagttagttt cattaatcat aatatctatc tcatttccat taactatatt gttggtgaca
    10441 ataggacttg ctgagcctaa atacaatata caaaaaactt ctacaaagag tgggattttt
    10501 gatatatctg atgattttta tgatagtaat atatttgacg aaggaacaat ggcaagtcta
    10561 ttacccaagt ttaagctatc tgtaggtggt aaattaacta gtaaagaaaa aggtaaattc
    10621 caacaagaag gtaaaagtat agatttaagt agtagtgatt ctgagtatga taaaatcatg
    10681 attaatgaac aagcttctga agaatattta agttatctaa aaaatataaa agtgtatgtt
    10741 attaatacat acacaggagt attttataca aatacgtcat atgctagttt atatgatttt
    10801 aaacaaaaaa ctaagagcta ttgtaatgta gagataataa ataagatagg aaagagctca
    10861 tatattaaaa caataaatgg agaaaaattt gaactgaata atctgagtct ggaactaaat
    10921 gaagtagatg aaggttttga ggcatatgta tcttttccag aagagccaac tatagaagat
    10981 ggaatagtat acactaattt tcaaatattt aaacaagcta cagaaaaagt aagattattt
    11041 tttgtagcta gtttaagttt agcaattata tgtttattat ctataatttt atatatcaaa
    11101 ataaaaggtg aagttttaaa aagaaataat gtatttttac taatatacag taagatacct
    11161 atggaattta atgtaatatt agtatctatt tcaattgcaa ttttaactat atatgctctt
    11221 gctgtatctt atcatattga tgtaattgca ttagtttctt tatctttact gatagtatca
    11281 aataatatat tttttataaa tgtgcaattg agctatttag aaaataaaat gtgtatattt
    11341 aagacaagta ttatagtaag agtgatttta tggttaataa aaactgtaag ggaggtcagt
    11401 aacgctataa agaaagtatc tttagcaagg aaagttattg ttatagtgat aatatcttta
    11461 gctttaatca tggcaattag cactcgaagt ataggtatgg gattactgtt ttctatatgt
    11521 atttttttag gatttacaat atatataact aaaagacttt catatcttag ttacgtaata
    11581 gaaggtacag aaagaataaa aaagggagaa ttagattata agataaaaat tactgggaat
    11641 gataatttta cgtctttagc tgaaaatata aataatattg gagaaggtct tgatagagct
    11701 atagaagagc aagtaaaaag tgaaagaatg aagtctgacc ttataacaaa tgtatctcat
    11761 gatttgaaaa ctccacttac ttctataata aattatgtag atttaattaa aaaagaagaa
    11821 tcaatacaac cagagtatat aaatgattat ataaatgtac ttgagtcaaa gtcaaaaaga
    11881 ctaaaattat tgatagaaga tttatttgaa gcgagtagag taagtagtgg caatatagaa
    11941 ttacaaatta gcaagattga tttagtgcaa ctattaagac aaagtatagg tgaacttgaa
    12001 gaaaaattat ctaaaaataa tttatattta aaattaaatg ttccaaatga taaggtgt
//
LOCUS       DAELTA010000108        12042 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.108, whole genome shotgun sequence.
ACCESSION   DAELTA010000108 DAELTA010000000
VERSION     DAELTA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.108"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            236..1210
                     /locus_tag="KPG13_002575"
     CDS             236..1210
                     /locus_tag="KPG13_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986278.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1326..2387)
                     /locus_tag="KPG13_002576"
     CDS             complement(1326..2387)
                     /locus_tag="KPG13_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG0986279.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(2999..3850)
                     /locus_tag="KPG13_002577"
     CDS             complement(2999..3850)
                     /locus_tag="KPG13_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG0986280.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3867..4787)
                     /locus_tag="KPG13_002578"
     CDS             complement(3867..4787)
                     /locus_tag="KPG13_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG0986281.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="KPG13_002579"
     CDS             complement(4897..5811)
                     /gene="murQ"
                     /locus_tag="KPG13_002579"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG0986282.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5910..7007)
                     /locus_tag="KPG13_002580"
     CDS             complement(5910..7007)
                     /locus_tag="KPG13_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG0986283.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7240..8586)
                     /locus_tag="KPG13_002581"
     CDS             complement(7240..8586)
                     /locus_tag="KPG13_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0986284.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8675..8983)
                     /locus_tag="KPG13_002582"
     CDS             complement(8675..8983)
                     /locus_tag="KPG13_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0986285.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9246..9644)
                     /locus_tag="KPG13_002583"
     CDS             complement(9246..9644)
                     /locus_tag="KPG13_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986286.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9646..10806)
                     /locus_tag="KPG13_002584"
     CDS             complement(9646..10806)
                     /locus_tag="KPG13_002584"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG0986287.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11237..>12042)
                     /locus_tag="KPG13_002585"
     CDS             complement(11237..>12042)
                     /locus_tag="KPG13_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986288.1"
                     /translation="IENSGEIDSYLVDGQSFEENLIKDIIENLKIKNNSDIETYKQSL
                     KELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSN
                     TMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQI
                     INNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQL
                     YKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         3844 a         2113 c         1323 g         4762 t
ORIGIN      
        1 ttcatttgct atttaaaatc aattttaagt tttcttctta taataaactt cctctttgtg
       61 gtctcatgtt agtctctaaa tttaaaattt tacaaaaaat tttcataaaa taaaattata
      121 ttgattgtat ctaaacaaat agggcaataa aataattaag tatagttact tacctttcaa
      181 gcaatattag ttttatgtta gaattttatt atattgattt acaggaggtt tgcatttgaa
      241 taatattaat tcacttaaag taataaaatt atctcttgga tgttctcttg ctatatttat
      301 tgcttggcta ttaaaattag aatattcaat ggttgctgga gttatagtac tacttacagt
      361 aaaggacact aaaagagaaa ctcttaaagg ctccattgga aagatttatg gttttctttt
      421 atgtactatt ttttcatatt tatgttttaa tattttagga tataatttga cttcattttc
      481 aatatttata tttataatta taccactttg ttttttgtta aatattcaag atgttattgc
      541 tatgtgtgta gtcatagcat cccattacta ccttcaagga gaaacttcta tcaaatggat
      601 tttaaatgaa gctggaatat ttgcagtagg aactggaata ggtgtcttaa taaatatgta
      661 tattccaaca aatatacata aaatccatga agttcaaaaa aagttacagg aagaagtgag
      721 tattgtactt atagacattg cagatattat tgtaaatcct aaaaaagaaa atggatatag
      781 tagagattta tatacattaa attcacttat tgacagcagt atttctgaaa cttacgataa
      841 tattaataat actttattaa gtgatactag attcttctta gagcatatgg atattataaa
      901 aagtcaacgg gatatcttag aaaatttgta tagttatgta tctcaactaa attctacacc
      961 accacaagct catattctat ctgcttttat acataagata ggttatactg aatttgaagc
     1021 tgaaacaggc aatcttcttc ttgaagaatt aaagagacta atgcttagta tgaaaaacca
     1081 gcctctacct gttgacagaa cagagtttga aaatagagct attttattcc tatgcttgac
     1141 tgagttaaaa cagtttttag ttaatcgtaa acatgctcag atgcttagag ataataattt
     1201 ttataaataa aaaggggaga aatctagcag ttgacctgac tacaaaatat cagatataat
     1261 attaaatgcg cccctagtaa gagggcgcaa tgtttataac ttattctact gtcatgatta
     1321 aatcattatt catatcgaac atacattatt tgaccaagtc caatattagc tttttcatca
     1381 gtgatttcta ttttaatagt cacatcattt tttttacagt acttcgctaa ttctgcacca
     1441 gaacgatttt taccactaaa atctaaagaa aatggcttat catatttaaa aggatattca
     1501 acaggtttac cctctggtgc taatgcaaac atcccatatg gtgtgttcat aaatcctatt
     1561 acagcaatcg taagatttga tgggtctaaa tttgattcac tacctgtaaa tgtaattgta
     1621 tttactgatg aattaactac actacatcga acaccttcat acattacatg tttttcattt
     1681 atatcttctc cttttttccc aaaatttcca acagcctttg ccccttcttg atgtggtaac
     1741 atagtgtgct tcccaggtgg tgctggtttc tgtgtctgtt ctggttttac cgtctgtact
     1801 attgcaccaa cttttggata tgctgtctta tcaaatggta atgggtggct aagactcttg
     1861 tatatgatat cctttcctgc tgatttatta gcatcaatat ctacatgata caaacgccca
     1921 tcaccaaaac ggataagatt ccactggtgg tcatttgatg tgctgtgtac ataataagat
     1981 tcaattccca tagcatcaca caaagctttc atgctcctag ccattcctga gcatgcacct
     2041 tttttagtaa ccaatgtata ggcagtattt gctccctcta ctgcattcca gtcaacatat
     2101 gttgtgttat ttttaatgtg attgtaggca actaaaatct tagcatctgt ggtcatattc
     2161 tttgtgattt tcttgttcat aaaatctgct gttgcttgtg ctactgagtt tagttgggct
     2221 tgtgacagct ttggaccata tacgctattg gttccagtga catagctttt tggtgttttt
     2281 gctgttacct ctttggctgt cataccataa actgtcatac tcatacctga gattaaaact
     2341 attgttgtaa tcaataactt ggtagctaac ttcctaattt tcttcatact taatattctc
     2401 ctttataaaa cattgttact tcctattctc taccattaat aaaattaatt taggatttaa
     2461 aaaggtggat tgtcaacatg tatatttctc cattcataca ttcgtgaaat atttaaattt
     2521 catggaaaat ctaatagata ttttctagat aatttgtata caatacaact cgcaaatcag
     2581 ggtgtttgct gttagtttga gaaaatgatg atgattaata cattcggaat ctttgaccta
     2641 taataaatac cattgtcttc aatcattaat aatggtaaga gtaaagagaa ttatcaataa
     2701 tacagttaaa atcaactttg tatacatttc atacattctc tcccaacaac attatacttc
     2761 ttataatttg tatattcaat acgttttctg ggaaaatata ggatatatct ataaaaatat
     2821 aaaaaaggag ttgaacttaa aatttgatta aaaaaaatca attttaagac aactcatttg
     2881 tattataaaa atgctatata ttattattaa gtaagtcaat attttagata tttatctatt
     2941 tcttattgac ttacgcttat agtctaactt atttgtttag ttgatatata acctagtttt
     3001 aagcattatt aatatttaaa taatcaagta tcctttttgt ttcttgtagt ttctgttctg
     3061 cctcatttaa gttttcttga attatagaaa tatatattat atctatcaac gtaagcattg
     3121 ctattcttga agaaatagct ccttctctaa gccttttttc tatatttggt actactagtt
     3181 ttaaatcact aatcttacta agatgattgt cactatattt agttatacaa atgaccttac
     3241 atccttttaa ttttgcattt tctatagact ttattacttc ttttgattct ccagagtaag
     3301 atattgctat agctatatct tctttatcta catttgaaga taccataagt tgtgtatgac
     3361 tatctaaaga ggtaaatgcc tgtttattta tccttaaaag tttcatctgt aaatctagtg
     3421 caactaaagc cgaaccacct actccaaata tatatacatt tttagccttt attatctgtt
     3481 caactgcttc ttttattata tctttttcat tcaacaacct tgtttggttg atagtctcta
     3541 tgtttttgtt tgtaattttg tttataacac tatctatatt acaatcatag ttttcatcta
     3601 catatgtgta ttcattttct ttgcccatca aatctttagc tatctcaatt tttaaatccc
     3661 caaaacccga atatcccatt tttctagaaa acctaactat acttgctgta cttgtttttg
     3721 agtttgacgc caattctgat acagaaagct tacttatagc ctctgtatta ttaagtatgt
     3781 aatcagctat ttttttatct acttccgtta aatcttcata tatctgtttt aattttaaaa
     3841 tgcaactcat actctatcac ctctacctat ttactataat atttcttctt agcaagtaaa
     3901 catgctccta aatctggagt caacactggt ctcactatac caaatctcgt tttattcaaa
     3961 tattttttaa gtggctctaa tattaaatct cctgacttaa acactccacc tgaataagat
     4021 acctttatct ttgtaccttg ttcaaaatga tgctctaatc ctattataag cctacttaat
     4081 tcataagctg catcatcaaa tattttacta gcagtattat caccttcaat aactgctttt
     4141 aaacatatcg tagcaaactt tgctatttct attctatctc cttttatttc atcatttaca
     4201 tattttatta tgtcataatc attctctata ttaagatgtc tttttattaa atcatataat
     4261 tttgtttttg aacaccttcc atcagactgc attgtgaaca tttttaaggt agccattcct
     4321 atataatacg ctgaaccttc atctcctaat acatagcccc atcctccaca tctgtgaaga
     4381 ttcccatctt tatctaaagc ctgtgctatt gaaccagtac ctgcaattat atttatacct
     4441 ttttccccat ttaaagaacc tgctaatgct atttctacat cattacctag agtataattt
     4501 atatgactat atgcattttt tgttgctact tctaatgcat acaatacttc ttttattttt
     4561 ccatatccag caagtccaac aaatgcatac gttatttctt ctacatttat cttagcatct
     4621 ttacaaactt cttccaaccc agttattaat aatttcgtta gattatcaaa tcctatttga
     4681 ttataatgac aagttccttt ggttatggtt cctactatat ttaattcttc atctactaat
     4741 gtaaatgttg ttttcgttcc tccaccatct acacctaaaa aatacatact atcctcctac
     4801 aatatcttta ataattcttt caatacttaa tatctaaaaa taaccttcca atgctgtttt
     4861 tttaatattg aaaggttatc agtactatta tttattttac aatttttcta ttgattttaa
     4921 agcttcactt atataacctt cttgtctatc taggatactc tttgcactat ccttatctaa
     4981 accacttttt atcataaata tagccaattt aacgctatta tctgcttctt ctaagaattt
     5041 ttctgcaaca tcatatttta catttgtagc tatcattatt attctttttg ctctttcaac
     5101 caacttttca tttgatgtct taacatctac catcaagttt gagtatactt ttcccatctt
     5161 aaccatagaa gctgttgtta acatattaca taccattttt tgagctgtac cacttttcat
     5221 tctagttgaa cccattatta cttctgcccc tactataatg tttattggta aatccgcttt
     5281 tttactcatt tcactattcg gattcatagt tattgatagt actgcacacc ccaattcctt
     5341 agcatactcc attgcaccta taacataagg agtccttcct gatgcagcta taccacatat
     5401 aacatcctta gatgtaactc ctctatctat tatatcttgc tttccaagtt ctttatcatc
     5461 ttcagcacct tctattgctt taaatattgc ttgatttcca ccagctatta tagcttgaac
     5521 aagctcaaaa tccactccgt aagttggagg acattctgtg gcatctagta ttcctatcct
     5581 cccactggta cctgctccta tatagaataa tctacctccc tgtttcattc tatctactat
     5641 tatattaaca gcttttgcta tactctcctt ttgttcttca acagcatagg ctacagtttt
     5701 atcttcgtta tttattaatt ctattatgcc taatgtatct tctttatcta tttgcaatgt
     5761 attcttattt ctaccttctg taactaaatt ttctaaagtt tttattaaca tatcatttcc
     5821 tcattttctt aatattaaat ttttaaacac attttaattc caactttcat aattatattt
     5881 ttataataaa ttaatatttt cataataaat tattttgttg agggtttaaa attaaacttt
     5941 tgccatggtt taatataatc taatataaat aattcttctt ttcttatata tcctactaca
     6001 ttcgatttac cactgttttt catatcagtc aaggctattt gtaactcccc tgcataatga
     6061 ccatattcac tactttcaat tactatatct cctcttttta ttattttagg agtattaaat
     6121 agttcaaatt tatatccttt atatttaaca cgagattgag tagaccttat cagactatca
     6181 gatatgtccc ctctattaaa atgtatttct tcaaagagta tcttcttctc aacttctcgt
     6241 atattttcct ctattgctac atcaaatgta accatatctt ttctcataga acctaatacc
     6301 tttagctcct catatgttgg ataacaattt gatattatta tatcatttat attattcata
     6361 gctattatat gctttacttg aatatctata ggtagatttc tgtgcatctc aattgttgga
     6421 agcccatcag ttacaggcca tggtccaaat gtagtttcat tttgacttgt tacaaaaact
     6481 gctgttctta atccatactt attaaatctt tctgtacatt ttataaagtg ttctaaattt
     6541 aatccactat atctatgtgg ataaaagtta tgacatccta tcaaattttc tttatttggt
     6601 tggtagtcca ttattgtatc tatataatta gtattattac tcatattgat ttctatttta
     6661 agattttgag gattaaaagt cattaatgcc tcttcagaac ctgtaaatcc catatcaagc
     6721 cttattccgt ctgccttcgt ctttttaaaa aaattcaaat ctttgtagct tatttctaat
     6781 tcaccaaata ctctgggatt tacatcaagt attacctcaa aacctttaaa cttagcgtat
     6841 tcatttatac ttttaaattt tttaattata ttttctttag tatctttaac tgataataaa
     6901 catgaaaata tacgtgtaaa tccattttca gcagctttat ctatataatc atatatttcc
     6961 ttagatgttg ttttttctgg ataaattgat attcctaatc ttcccatctg tgtcatctcc
     7021 ttatatccta tatgtgttta aatttatttc gaattacacg aaactttttt aaaaatttga
     7081 tgaaaaaaag tttcatatat atttataaaa aaagaaaagt ttaccttttc tttttttata
     7141 aacaatcaac aaaatatctt cgttacttat ttcataactg ttatactttg aaatttatac
     7201 taatctaata tagtttttaa tttccaatat agtttctaat taataagctt cttgctcttc
     7261 aactttttct tcatgaagca acctcttatc gtacatcttg acaaatggat agtaaattat
     7321 tattgatata gctatacata ataagttaag aactgaagct ctccaatctc caccagtggc
     7381 taaatatgcc cctactggtc ctggtaatgt ccaaggagca gtaactacta ctctggatac
     7441 taaattagca ctcatagcaa accatgaaac tgttgccatt actattggtg ctacaacaaa
     7501 tggtattatt aatattgggt ttaatactat tggcgctcca aatattattg gttcatttat
     7561 attgaatatt gctggtatta cagttgcttt tcctaaagac tttccataag ctgattttgc
     7621 tctaaatgca agaagtaaag ctaagcctat tgtagctcca gaacctccaa tccatataaa
     7681 ccattgaaaa aagggttcag cagcaatacc tggaactgct tgacccgcag catatgaagc
     7741 tgtattagca tctaataatt gtatccataa aggtcttgcc aatgaaccta ttattgatac
     7801 accatgtatt ccaaatgacc aaaaaaacgt tattaaaaat attagtataa ttactgatgg
     7861 taatgtgtca gtggctgaaa ctaatggagt tactaattta gctatgaagc catgccaatc
     7921 aaaccctagg aaataagtaa caatagccat taaaattatt attaatgcgg ctggtgtcag
     7981 agcttcaaaa gaccttgtga ctgatgctgg aactgcttct ggcatggtta tcttgaattt
     8041 agatttgtca gtaaatctat aaatctcaac acctattata gatgttacaa tagcaacaaa
     8101 cattgaacct ccaccaagat tagccattgg catttgccat ccttgaggaa ttgaattata
     8161 ccaagctaaa agttctggac tcttttcaac tatctttaat aaatcagggg taagaccctc
     8221 tattgcagta ggaactattg tcaaaaggaa tgtagctgta gctaataaac ctcctgtcaa
     8281 tccatctaac ttatatgact ttgccaaact ataacctatg ccaaatgtag aatatactga
     8341 tataataaaa aatgtcattc tatatggtag taatattgta actgcatttt cctttagata
     8401 ctgagttacc ccccatgaat ctggtaatgg aggaaatgct aatatcaaga aaaatgaccc
     8461 aactattata agtggtaatg aagctacgat tccatctctt acagctctta agtgacgttg
     8521 ttctgccaat ctggccatag aagttgataa tttagtgtct aaaaattctg taaatttatc
     8581 tagcatcttt tctctcctta attaatattt atttttatta tcattaaaat ttttataaaa
     8641 ttaactttct tctaatctaa caacactgca actactattt actcaacagc tcttgaactt
     8701 gttttaataa ctttggtcct cctaatggac tataagcttg tgcttgtatt aatgcacatg
     8761 gtacactaac ctcatcagca aaagctttta aatagtcaaa tctatgtcta acttgtggag
     8821 ctaccatagc aatatcccat ccatttttaa cttcatcctc aaactcttga gtaccaacag
     8881 ccaaaacatc tatatctatt ccattttttg ctccttcttt ttgtaatgca ttaactgcta
     8941 tagcacttga catccctgat gaacaaacaa ataatactct catattgatt ccccctgttt
     9001 ttttatttct tttcaatcat tatagcatgt atttttgatt acaggaatac gtttgtatat
     9061 atttttttca tattttacat tttaaattgt aattttattt catacattta tatcttgctt
     9121 taattgcttt atttatctaa tcattctttt gtctaaaatt acacactact taaaatactc
     9181 atatcttttc ttaaaaaaga aaaaagacta tcttattatt aacataaaat agtctcaata
     9241 cttttttaaa tatgtattcc tttatatatc cagaagtaac ctattattgc aaatgataaa
     9301 gcaaataaaa tatacagtct aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag
     9361 tatcttgtat atgcaactgc aaaagatgta attaatgaat ttcctataac aactccaaat
     9421 ctattatcta ttccaaatat agataataac aaaggaaaaa ctacagtact tactagagat
     9481 gaaactatta atattaatcc acttttaaaa cttatagttg gaatattata atctgttctt
     9541 tgatttttct ccatttgttt cttagtattt ttattacttt ttgtactttt tgcattttta
     9601 taattattat ttttctttct tttctttttt ttattattag acatgtcatt tacctccaaa
     9661 aggtttaggt gttatattcc ctaaaataac tttattttta gcccctgttg cagatattac
     9721 attagaaaaa gaattattta atgtttcatt gcccaagatt acaaatgcca aagcctcctt
     9781 tgcatcagaa gaatacccat attcatcttg agttaatact tcaacctcta ataataactc
     9841 ttttatatat cctattaaag ttagattatg agctcctcca cctccaatta ataaagtatc
     9901 tacattaacc tttggaagta tgaagttttt ataattataa tatatagctt ttgccgtaaa
     9961 catagttact gttgctatca aatcatattt atctacatgt ttatatttgt taagcatgtt
    10021 atccaaataa acttgtccaa atacctctct tccagtgctt ttaggtggcg cttgactaag
    10081 atacttatga ctcatcaaat cttttaacat ttcttcatta attttacctt ttgatgcaaa
    10141 gtatccattt ttatcatact ttctattaaa taatctttga catactccat ctattatcat
    10201 atttcctggt cctgtatcaa atgcaaatac atcatctatg ttacatgatt taggtattat
    10261 agtaatattt cctattccac caatattttg aagtgctaaa tttttattgt tatcactata
    10321 taataaaaat tcactatatg gtactaatgg agcaccttct ccacctgctg ctatatccat
    10381 tactctaaaa ttagatatta cctttgtatt tgtttcatat gctataactg ctggttcacc
    10441 tatttgaagc gttgaagata caaaattatt ataacttctt ggtatatgaa agattgtttg
    10501 accatgagat gcgataaaat ccaaatttgc tatatgaaag tttgctttat gacacactga
    10561 ttttacagct ttactaaata aatatccaag cttaaaattt aagctacaga ttaaatcaac
    10621 actcgattcc tcttctatac agcactttct tatttcgtct tttatatctt taggaatttc
    10681 tagagttata aattctattt gcttaacttt agtatttcta ccattcccac taatttcagc
    10741 tagtactgca tcaactccat ctaaggaagt tcctgacatt aaacctacac tatacatagt
    10801 tatcatcctt ttaagtttat agttattcct ccccaaatca aggtattaag tttaataatt
    10861 ttagaattaa tcttatttca tctttaatta cgataattta ctcttatgaa attaattgag
    10921 ttaatcttat acacgttttt taccttagct aaatcttaca ttaatttatt ttcagtgtca
    10981 atgtaatacc atgtaatttt ttttcacaaa caaaacatat tctctctcca aaaaccatat
    11041 taaaatagtt actacaaata aaaaacatac caggtaccta tctgatatgt ttttcatata
    11101 taataattat tattttataa tatagataga gcatcattta aggcactaat taaatcttca
    11161 ctattttcaa gaactaaatt tattcttatt gtatatgtcg atttgtatat gatgatagat
    11221 tttatagtct aataacctaa ctatctaggc ttaaataatc cgatatatca taaaaataga
    11281 tattaaaaat tgtatatgaa ccattttccg aacatacctc aatctcatgc cctaaactat
    11341 ctatcatttt ctttacaaaa tacatcccca ttccagtaga cttatacaat tgatttcttc
    11401 catttttccc cgtaaatccc ttgtcaaaaa ccctagatat atcttcctca tttataccta
    11461 taccaaaatc ttttatagat aataccactc cattttctaa cttttttgaa ttaaattcaa
    11521 tcttaccaac ttcctttgaa tactttatag cattattaat aatttggtct aaaacatatg
    11581 acatgcattt agaatctgtg taaacatcat tttctaattc attcaaacta acttctacat
    11641 tattttttat aagcaaaaat gaattatttt ttatagacat ttttatcaat ttttctaaat
    11701 taaatttatt tataaataca tcttcaaaca tagtgttgct tctactacta tacaaaatag
    11761 agtttactaa aaaatttatc tttgcaacct gattcttaat atccaaacta tcttctatgc
    11821 tacttaacct atctgtaatt atacttaaag aagatattgg aatttttatt tcatgaaccc
    11881 atttagctat gtactcatcc aattctttta atgattgttt ataagtctct atgtcactat
    11941 tattttttat ttttaagttt tctataatat cttttattag attttcttca aaagattgtc
    12001 catcaactaa ataactgtct atttcaccac tattttctat ac
//
LOCUS       DAELTA010000109        12006 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.109, whole genome shotgun sequence.
ACCESSION   DAELTA010000109 DAELTA010000000
VERSION     DAELTA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.109"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            258..911
                     /locus_tag="KPG13_002586"
     CDS             258..911
                     /locus_tag="KPG13_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986289.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            1007..1606
                     /locus_tag="KPG13_002587"
     CDS             1007..1606
                     /locus_tag="KPG13_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HBG0986290.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
     gene            2196..3671
                     /locus_tag="KPG13_002588"
     CDS             2196..3671
                     /locus_tag="KPG13_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0986291.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            3758..4171
                     /gene="speD"
                     /locus_tag="KPG13_002589"
     CDS             3758..4171
                     /gene="speD"
                     /locus_tag="KPG13_002589"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG0986292.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            4201..5052
                     /gene="speE"
                     /locus_tag="KPG13_002590"
     CDS             4201..5052
                     /gene="speE"
                     /locus_tag="KPG13_002590"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG0986293.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            5042..5920
                     /gene="speB"
                     /locus_tag="KPG13_002591"
     CDS             5042..5920
                     /gene="speB"
                     /locus_tag="KPG13_002591"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG0986294.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(6100..6300)
                     /locus_tag="KPG13_002592"
     CDS             complement(6100..6300)
                     /locus_tag="KPG13_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0986295.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            6767..8257
                     /locus_tag="KPG13_002593"
     CDS             6767..8257
                     /locus_tag="KPG13_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0986296.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            8537..10033
                     /locus_tag="KPG13_002594"
     CDS             8537..10033
                     /locus_tag="KPG13_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0986297.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            10167..11093
                     /locus_tag="KPG13_002595"
     CDS             10167..11093
                     /locus_tag="KPG13_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986298.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            11211..11402
                     /locus_tag="KPG13_002596"
     CDS             11211..11402
                     /locus_tag="KPG13_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986299.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            11625..11975
                     /locus_tag="KPG13_002597"
     CDS             11625..11975
                     /locus_tag="KPG13_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986300.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         4837 a         1234 c         2124 g         3811 t
ORIGIN      
        1 tagagtaaat taaatatctt tttttagaaa aataaataga aaataaggtg aatgtggaaa
       61 gagttttgtt ttatcaaaac tttttctact ttttttgtat aaattatcat aagaatttta
      121 gtatttcaaa atattacaaa gaagtataaa aaatatcata attgtgatat tatataaatt
      181 agtaagataa aactgaactc tctaccaaag ttgtagggag atttaacttt tataatctta
      241 atatggagga acgtcttatg aaggtagatc cacttacaac tcaagtatat gactatatat
      301 caaagaaaat ccaaaatgga gaatatgaag ctaatcagag aattactgaa tctgagattt
      361 gtaaatctat cggagttagt agaacaccag cgagagaagc attaactagg ttagcaggtg
      421 aaaatttact agaaaaaata cctaataagg gatttgtagt aaaagaattt caggaaaagg
      481 agaaattaga cacctattcc gtaattggag tattggatgc gttagccggt tcttcagcat
      541 tacaaaattt aactgaatca gacctagtaa aaatggaaga attaacagaa atgatggcgg
      601 tctctataaa gtataaaaat tacaatagct atttaaaatt aagtaatgaa tttcacgata
      661 tatacataac taaatcagat aatcaggtgt tagtaaatct attgaactct ttaagatata
      721 attttatgtc aaagtcatat actagtgata atgaagatga gttatataaa atgttgacat
      781 acagcaataa tcaacatatt gaagtagtaa aatctatgag ggagaatgat ttagaaaatg
      841 ttgaaagaat tttgagagaa cattggaaaa cgatacctat ggaagagatg aaaaaagaga
      901 agcttttata aagtttctct ttatttttta taccatttta tcactatttt tttgaattat
      961 taagtagaaa ataataatta atttaaactt gaggaggaaa actaatatgt atccaaataa
     1021 agaaattgca atagaagagt tagaagtggc tggcaaaatg aatcccggtc catggataaa
     1081 acattcttac aatgttgcta atgtagctaa attgattgca gagcaatgtg aaaatttgaa
     1141 tagtgaaaag gcctttgtat gtggattatt acatgacatt ggaagaagaa ctggtatttc
     1201 ttctgtaagg catattattg atggatatga ttatgttatt tctaagggat gggatgaagt
     1261 ggcgagggta tgtttgacac attcttttcc tgttaaggat atagaagctg atattggaaa
     1321 taaagatatt agtaaagaac agtatgattt tatcaatcac tatttaaaca accttgaata
     1381 taacgattat gataagttga ttattctttg tgatgcatta gcagatgcaa atgggttttg
     1441 tattttagaa aaaagatttg ttgatacaac acgaagatat ggaatatatc cattttcagt
     1501 tgaacgatgg aataagactt atgagtataa agaatatttc gagaaaataa tcaacaagtc
     1561 tatatataca ttgttaccca atattgaaaa atctatatat tattaaatcc taagttaata
     1621 atttaatatc cagtatcacc agcagttggt cattatgatt gactgctttt attatgctta
     1681 tattaaaaaa atacactatg gtttgtgtat atcaaagtta tatggaatga aaagagaatt
     1741 tattttatca attaaaaaat ttaataaatt ttatattaaa attaaaatac aaaaatagga
     1801 aaattattta gtccaactaa accaagaatt ttgatatgtg aaataagaat aatataaaag
     1861 tttagtaata aaatacttat aaattaaaaa tagtaaactc aaaagcttac taaaactcaa
     1921 aaaagtaaaa aaaacaaaat aataaaaaat aagaaagttt ataaatacta agttttaaaa
     1981 aaattaatat aaacaacaaa aaaataaaaa gtttataaat attgatatag ctaatgtata
     2041 tgtttttgag taacttcaaa aaaacacttg taaaaatgct gagatgttaa tataataaat
     2101 atcatgagtt taataaaagt aaaaataaaa aatagaaata ataaataata aatattaaaa
     2161 atatttgaat atagagagga gatgaatttg aaatcatgga tagcgagttt gcaaatgcag
     2221 ttgaattaag ctatgagtgt gctccattat tagagtcgtt aaaggaatac tcagagaaag
     2281 atatagcttg ttttgatgtg cctggacatg taaaaaatag aggtgtagct atattaaata
     2341 aatatctagg tgaaagtctc atgaaaatgg atataaattc atcaccaact atggataatg
     2401 tatctgcgcc aaatgggatt ataaaaaatg cacaagattt gctagcacaa gcatatatgg
     2461 ctgatgaggc attctttata acgaatggca caactcaagc aatacatgca atgattttga
     2521 gtgttataaa accaggtgaa aaagtattac ttccaagaaa tatacataaa tcagtaataa
     2581 atgcgttaat actttgtgga ggaataccaa tatttataca accagaattt gatgaaaaac
     2641 taggtataag tttaaatatt actcttgaaa aagttagaac agagatagaa aaagattgta
     2701 atataaaagc attatttttc cttaatccaa cttactatgg agtttgtgca gaccttgaga
     2761 gtattataga actatgccat aaaaataatg ttttagtgtt agttgatgaa gctcatggtg
     2821 cacattttcc atttcattta gatttacctc cttctgcaat aagtttggga gctgatatgg
     2881 tagctgtaag tatacataaa actggaggag cattgacaca atcttcagca ctcttattga
     2941 atagagataa tgtaagtttt gaaaaggttc ttcaatcaat caatatgcta caatcaacat
     3001 cagcatctta tttgcttatg gcaagtatag atggtgctag agttaatcta gttgagaatg
     3061 gtgaaaagca actgtcaaaa gcattaaatc tatctaggta tgcaaaatct aaattaaaca
     3121 aaattgatgg aataaaagtt ttatctacag aaatattgaa acaaaaaggt gttaaattta
     3181 tagatgaaac aaaattgtgt ataaatgtaa aagaattaaa tttgactgga tttgaagtat
     3241 acgatttatt atataaaaat ttttcaattc aagtagaatt gggagattca tataatatac
     3301 tggcacttgt gtccattgga acaaataaat cagatataga tagattagta aaagcacttt
     3361 ctataattgc aaagatttat agaaaagaat caacattaaa tgaatttaat atggttcaaa
     3421 taaatccaat tattaaactt aatccaagag aagcttttta tgcaccaaag gaaagtgtag
     3481 aaatcaattc atgtatagat agaatttgtg gagaatcaat aatggcatat ccaccaggta
     3541 taccgattat tgctccagga gaattgataa cagaagaaat tatggagtat attattttct
     3601 taaaaaatag caatgcatat cttacagatg tacaagataa aaacttagat agaatattag
     3661 taataaaata aattacttta ttttgcaagg gcaagtattt gttaagtaga gtttatcaaa
     3721 tagaacttat taaaagtata aaggagttgg ggttgaaatg aaatttgaaa cactaggaag
     3781 acatatacta gtagaatact ataattgtga tgaaaacgta cttaaagacc cacgtcttat
     3841 agaaaatttt atgaatgatt cagcattaaa tgcaaaagcg acaattgtgg attctgtatt
     3901 ccaccatttt aatccttggg gggtttcagg agctgttata attgctgaat ctcatttgac
     3961 aatacataca tggcctgaat atggctatgc agcagcagat ttttttactt gtggtgatat
     4021 agacccatgg aaaagttttg aattattaga gcaactttta aagtctgaaa gaagcgagtc
     4081 tacagaaatt ccaagaggct taactacaaa aattaaaaaa tattcaaaaa aagatttagg
     4141 taagattact cataaaccag aagcagtata aaacatacaa gagaaagtga ggattattat
     4201 atggaacttt ggtatacaga agaatggaca gaaaatgtaa gattttctat aaaagtaaat
     4261 aaacatctat ttgaaggaaa gtctcaattt caaaggatag atgtgtttga tagtgatgaa
     4321 tttggaaaat ttctaacaat agatggatta atgatggtaa catataaaga tgaatttata
     4381 taccatgaga tgataactca tgttcctatg gctacaaatt taaacataaa gaaagtatta
     4441 gtcataggtg gaggtgatgg aggtacagta agagaattaa gccgttatcc tcaaatagaa
     4501 aagattgaca tggttgaaat agataaaatg gttgtagatg tatctaagga gtatatggat
     4561 atatgctcat gtaaattaga tgataaaaga gtgagtttat attttgaaga tggtgttaat
     4621 tttgttaagt gtgcacatga taaatcatat gacctgataa tagtagattc aactgaccca
     4681 ataggaccag gtgaagggct tttctcaact gatttttata aggattgtta tagaatactg
     4741 actgatgatg gaattttagt aaatcaaagt gaaagtccgt attttgactt taatgctaaa
     4801 gagatgaaaa gagctaataa aaaattaaaa cagatatttc caatatcaga agtttatcaa
     4861 gcacatatac caacatatcc atctggtcac tggttgtttg gatttgcttc aaaaaaactt
     4921 aatccagtaa aaaatcaaga tagaaatgga tgggagaaat taagtttaaa aactaaatat
     4981 tataatagtg atatacattt aggttcgttt atgttacctc aatacgtgaa ggagatgcta
     5041 gatgaagaat aatttctatc atatgaacac ttttatgagt atggacaaaa attatgaaga
     5101 atctaatctt atagtatttg gtgttggatt tgatggaact acttctaaca gacctggggc
     5161 tagatttgca agtagctcta tgagaaaaga attttacggt cttgagacat atagtccttt
     5221 tttagatttg gatttagagg attataatat atgtgattat ggagatttag aaattagtgt
     5281 tggaagtaca gaacaagtct taaaagaaat ctatcaagag acatataaga ttgttagaga
     5341 ttcaaaggta ccctttatga ttggaggaga gcatttagtt acattaccag cctttaaagc
     5401 agtacatgaa aagtacaatg atatatatgt aattcatttt gatgcccata ctgatttgag
     5461 ggaagaatat aataatagta aaaattctca tgcaacagta attaaaagaa tatgggatat
     5521 tgtaggtgat aataaaatat ttcaatttgg tataaggtct gggacaaaag aagaatttaa
     5581 atttgctaca gaagaaaaac acacatacat ggaaatagga ggaatagata cgtttgaaaa
     5641 tatagttaat atgctaaatg gaaagaatat ttatctaact atagatttag atgtattgga
     5701 tgcatctgtg tttccaggaa caggtacacc agaacctggt ggtgtaaatt atagagagtt
     5761 tcaagagatt tttaagatta taaaaaactc taatataaat atagttggtt gtgacattgt
     5821 agagttaagc cctgattacg atacaacagg tgtatcgaca gttatagctt gtaaaatctt
     5881 aagagagtta tgcttaataa tatctgataa aattaaatag agtattttat ataaatagta
     5941 tatattataa tataagaagt tattttaaaa aagttagttt aagtttgacg cttttctaaa
     6001 gtaacttctt atttttgtaa ttaaaaataa atataataaa caacaaaaaa gagccctcga
     6061 tagctccttt tctaaaatat atatatttaa attaaataat tatattctat ttacgttttc
     6121 agcttgaggt cctctattac cttgagttat atcaaagcta accttttcgc cttcttctaa
     6181 agttttgtat ccatcatttt gtatagctga gaaatgtacg aaaacatcat cttctccctc
     6241 tacagatatg aacccaaatc ctttttcatt gttaaaccat tttactattc cgtttttcat
     6301 tataagaaaa cctccgcatt attaaaaaat acaaactaaa aaaataagtc tgttctatta
     6361 atataacata aattttaaat aaaagcaaat aaaattttta aaaaaattaa ttacttttag
     6421 aatttaataa aaattgttaa attatacagt gagaagtatt gtaaaatttg acgaaaatgt
     6481 aaatgtagat aataaattta atatcaaaaa atgtaacaaa aaggtatgta agtaaaaagt
     6541 aaaattaaaa tatataataa aaaatattat atattagatt gaaaagtaaa aataaaaata
     6601 gtgaaatttc agaaatatca ataatattat ataataaaac aaaaaaacac tatatatatt
     6661 cgataaaata aaatttaaac atataatatt ttatgtatat attaataaaa ggacatattg
     6721 tgttataatg gtatcgtata aaatcattta cgggggtggt attaaaatgt ttcaatttga
     6781 aacacaaata cacaaattaa gacatgatgt tttaactgca atagctagac ttggaaaaga
     6841 agataacctt accagagaag caatggataa tattcagtat gaaataataa aaggtgagaa
     6901 acctacatac agatgttgtg tttataaaga aagagctata atatctgaga gagtaaatgt
     6961 aagtatgggg ttacaaccag gtcaagataa gatagattta gatacaatgg atatagaaga
     7021 agatgaacaa attatatata ttttggaagc tgcatgtgac agttgtccaa taaatgaatt
     7081 tacagtaaca gatatttgta gaggatgtct agcacataga tgtaaagaag cttgtaaatt
     7141 tggagctata acacatgtcg gaggaatggc ctatataaat catgaattat gtaaggcttg
     7201 tggaatgtgt aaaaaagctt gtcaatatga tgccatctca gaggttgtta gaccatgtaa
     7261 gagtgtttgt ccaactaatg ccttaggatt tgatagagaa aatatgaaag ctatgataca
     7321 tgaggataaa tgtttaaact gtggagcatg tatgtcagca tgtccatttg gagcaatatc
     7381 tgataagagt ttgatagctc ctgtagctag aaagttagtt caaaaagaga aaatgtatgc
     7441 ggtggtagct ccagctataa caggtcaagt tgaagcaaat gttacatatg gtcaaataaa
     7501 aaatgctata aaatcactag gatttgttga tgtattagaa gcagcttgtg gagcagatgc
     7561 agttacagtt catgaaagct tagaatttgc tgaaagaatg gaaaagggag aagcttttat
     7621 gacaaattct tgttgtccag gatttgttgg ttatatagaa aaaacatacc caaatcatgt
     7681 agggaaaata tcttctacag tatctcctat gatagctagt ggtagattta taaaaagtca
     7741 tgaaccagat gctaaagttg tattcatagg accatgtaca gccaaaaaga gtgaggcagc
     7801 taagaaagaa ttaaaaggag ctgttgatta tgtacttact tttgaagaaa taatggcatt
     7861 atttgaggca tttgatatag acttggctaa gtgttcaaat gaagatatag atgatgcatc
     7921 aatttttgga agaggttttg gagctgctgg tggtcttaca aaagctatag aaaattatct
     7981 tgctgaaaaa ggtattggtg ttaattttga acctgtaaag atttctggtt caagagaaat
     8041 caagaaaact atgactatgg ctaatttagg aaagctagaa ggtaatttta tagaaggaat
     8101 gatgtgtgaa ggtggttgta taaatggttc tggtaaattc ttatcaggtg caaaggttaa
     8161 agccactttt gatagaaaaa attctcaatc aactaagaaa agtgtcttag caaatacaaa
     8221 gataaaagat tttagtaata taaatttaga aaaataaaga taatacaaag caacaaaaat
     8281 acatattgtt aatagagttg ttctaagttg aggtttaact tttggacaac tctattttta
     8341 tttatatatt taattattga acaactaaaa taaaaattat atacacttaa aataaaaatt
     8401 acatatataa tattttgtat ataacttaac aaagtatatg cctatgttat aataatttat
     8461 agaaataaat aaaaaataca atatacataa tagtagaaat atagcaaaaa ggtgtgattt
     8521 atagggagga ttacttatgt ttgaatttga aacgcaacta aaaaggttaa aacatgaagt
     8581 tttaacagca gtagcaagac tatctattga agaaaattta acaaaagaaa atatagaaaa
     8641 aattccatat gaaataatta aaggagaaaa ggcgagatat agatgttgtg tttataaaga
     8701 gagagaagta gttcttgaaa gggcgcaact tgcattaagt ttaactccaa atagtaaata
     8761 tggagatata aatccagatt cagttagttt ggacacagat gagcaaatta tatatatagt
     8821 tgaagcagca tgtgatagat gtccaataaa tgaatttaca gttacagagc tttgtagagg
     8881 gtgtttggct catagatgta aggaagcatg taaatttggg gcaatttctt atataaatgg
     8941 gagagcatat ataaatcatg atttatgtaa ggcttgtgga atgtgcaaaa gttcatgtca
     9001 atatgatgct atatcagaag ttgtaagacc ttgtaaaagc gtttgtccaa caggagcttt
     9061 ggattttaat agaaacacta tgaaagcaat gatacatgaa gataattgta taaattgtgg
     9121 agcatgtatg tcagcatgtc cttttggagc aatatctgat aagagtttga tagcacctgt
     9181 tgcaaaaaaa cttgcaaaga aagaaaatat gtatgcaata gtagctccag ctataacagg
     9241 tcaaatagat agtaatatta catatggtca gttaaaaaat gctataaaat cactgggatt
     9301 tgtagatatg atagaagctt cttgtggagc agatgcagtt acagttcatg aaagcaatga
     9361 acttattgaa agaataaaat caggagataa ttacatgaca aattcatgtt gtccgggatt
     9421 ttttaagtat atagagaaga tgttcccaga tgaaaaagat agaatatcat ctacagtgtc
     9481 tcctatggta gcaacaggaa gattagtaaa aaaaatagat tctgatgcta aagttgtatt
     9541 tataggtcca tgtacagcta aaaagagtga ggcaacagag aaaagtttaa aagattcagt
     9601 agattatgta cttacttttg aagaactaat ggcattattt gaagcattta atatagattt
     9661 atcaaaatgt tcaaatgaag atataaatga tggttcgatt tttggaaggg gatttggagc
     9721 atctggtgga cttgccaaat cagtacagaa ctatattgct gaaaaggggc ttagttttga
     9781 ttttgaacct ataaaagtat ctggtccaag acaaattaaa aagacaatga caatggctaa
     9841 attgggaaga ttagaaggta attttataga aggaatgatg tgtgaagaag gttgtattaa
     9901 tggagccggt aagtttttat caggtgtaaa agccaaaaat acttttgata gaaaaaatgg
     9961 acaatctact aaaaagagtg ttttatctaa tgataagatt aaagaatttg aagatgtaaa
    10021 tttagaaaga taattgtttt ggtacagtca tttttttatt atgccacaaa tatgcctaaa
    10081 atttgccctt tttatatgtg tattttacag agttaaggtt gctatactat aaatatgtaa
    10141 taaataaaat ttataggagc ttttggatga ggaaaatagt attaaaagat atcctgtttt
    10201 tagttactac attatctata atagtaatat tattgcttgt aacaaatata actactaata
    10261 gtaagataaa atcaacaagt gaatcttatt taaaaagtga caaaaatagt gtgaggcaaa
    10321 atggtatcca gaggagcaca aagagtagca caaaaaatac tgcatttaag actactatac
    10381 taaacactgg aaagtctgat tgcatattgg ttgagatagg taacaaagtc ataatgattg
    10441 atacaggaga agataaaaat ggaaaacaaa tagtggatag gttaaaagaa aaaggaataa
    10501 atacacttga ctatttgatt ttaactcatt tagataagga ccatataggt ggagtagata
    10561 gtgtgttatc tagtgttaaa gtaaagaata ttatacaagc aaattataag aaagatagta
    10621 agcaatatga tgaatatatt gactcactta aaaaagctga tatagaacca gtgctactaa
    10681 aagataatat gaatattgta atcaatggtg cagaaataaa catacatccg gcttcaaaaa
    10741 gcaagtacga gagttcaaat gattattcaa taatcactaa tataagctat ggagcacata
    10801 agtttttatt tgcaggagat gcagaggaag aaagattgtc agagtttata aatggaaata
    10861 ctttaaaata tgattttgtt aaaatgcctc atcatggaag atatgacaaa ttaactgaaa
    10921 catttttaga gtctatttca cctcaatatg ctgttattac atgttcagag aaaaaagaac
    10981 cagaggaagg tgttttaaaa atattggaaa gattgaatat aaaaacattt ttgacttcta
    11041 atggagaggt agttatcaat agtgatggta agacattgag tgtgaatcaa taattttttt
    11101 aaaaatatct aaaaaaattt aaaaaaatca ttagcaaata ttacccctta aacatagtat
    11161 attttaggag ctgaaactcc acatacaaaa tgactaggag gtaacatatt atgttggata
    11221 gattttttgg tgaaggcgga tttggtggat gtgcttggtg gataataata ttattcttct
    11281 tattcttagc attccaagat tgttgggctg aaatggactt atgtgcatgg atacctttct
    11341 taatattatt attaatactt tgtgaatgtg gtgacgtttt tgatggagga tgtggatgct
    11401 aaaaaaatca atttagatga tttaataaag cttatggtgg gtataaataa aataccaaac
    11461 caatcacaat ctagtgtttg aaggtagtcg gaaatccccc gctaccttct tttttttatt
    11521 gttaaaattt atttttgaat tatttcattt acatataata atttttacga taaagtctga
    11581 tataattaaa aagaaaataa aatttattta taggagatgt aaaaatggct atagtggata
    11641 agattttcaa tgagataaaa cttatggttg caagactttt attgggaaaa aaatacagtg
    11701 aatatgaaga aaaagattta gtgtataata cagaagaaga agttatactt ataactttaa
    11761 aaagattagt ttttcaggga aatgtaaacg aagcagaaga aattttattt gataaagcta
    11821 agagtgtaaa tagtgaaaat atgcagtata tagcaataga attttatact atgcttatgg
    11881 aaaagactga tgaagaatta gaagctatga atttttctaa acaagaagtt tatcaaggta
    11941 tagaagatat tagaaaagta ttgaatttag aataaaagat atatgaaaat attgaaataa
    12001 attaat
//
LOCUS       DAELTA010000110        11996 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.110, whole genome shotgun sequence.
ACCESSION   DAELTA010000110 DAELTA010000000
VERSION     DAELTA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.110"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            133..1014
                     /locus_tag="KPG13_002598"
     CDS             133..1014
                     /locus_tag="KPG13_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG0986301.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1075..2283)
                     /locus_tag="KPG13_002599"
     CDS             complement(1075..2283)
                     /locus_tag="KPG13_002599"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG0986302.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2356..2608)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2760..3749)
                     /locus_tag="KPG13_002600"
     CDS             complement(2760..3749)
                     /locus_tag="KPG13_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG0986303.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3901..4341
                     /locus_tag="KPG13_002601"
     CDS             3901..4341
                     /locus_tag="KPG13_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0986304.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4668..4895
                     /locus_tag="KPG13_002602"
     CDS             4668..4895
                     /locus_tag="KPG13_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0986305.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4921..7041
                     /gene="feoB"
                     /locus_tag="KPG13_002603"
     CDS             4921..7041
                     /gene="feoB"
                     /locus_tag="KPG13_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0986306.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSRLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7174..7992)
                     /locus_tag="KPG13_002604"
     CDS             complement(7174..7992)
                     /locus_tag="KPG13_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0986307.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            8199..8483
                     /locus_tag="KPG13_002605"
     CDS             8199..8483
                     /locus_tag="KPG13_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986308.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   8651..8877
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8718..8746
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(9536..11629)
                     /locus_tag="KPG13_002606"
     CDS             complement(9536..11629)
                     /locus_tag="KPG13_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0986309.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         4268 a         1765 c         1429 g         4534 t
ORIGIN      
        1 tgcaaataat gtcatataag ttacacttta gttatcttag ttacaacttt gtcggtttac
       61 tttttctact ttcttcgata taattataat atatttttga tacaattcga caaaaggggg
      121 aatgctgtaa ttatggtaaa aaaaagaaaa aagaaaactg ttacacttac tatacttgtt
      181 gtaatagtta ttggtatttt ttctatggca tgggcggaca tgtctagaaa aaaagaaatg
      241 aaagaaactc aagaaagact taaagctgaa agattagaaa aagaaagaat tgaaaaagaa
      301 aaaaatgaac ctattattgg tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca
      361 aaaatagttt gggacaaact taacaaatac gactattcaa acaatggaga aaagatagtc
      421 tttcttacat ttgatgatgg accatctact actaatactc cacaagttct agatatcttg
      481 aaaagacatg gtgttagagg aactttcttt attaagggtg attctcttga aagaaaagga
      541 gctaatgaaa ttttaaaaag aacatttgac gaaggaaatg caatagccca tcactcttat
      601 actcatgact ataaaaaact atatcctaac agaagcctaa atctcgatac attcgtaaat
      661 gaacttaaca aaactgatga agctatgaaa aaagtcttag gaaagaattt ttcctcaaat
      721 gtagttagat gcccaggcgg gtatatgtca tggaaaaata tggaacctct aggaaactac
      781 ttaaaagaga aaaacatggc atcaattgat tggaacgctt taaatgctga tgcagaaggt
      841 aaaaagaaaa atgcacaaga attatttgaa catgcaaaga aatcttctga aggcaaagaa
      901 atggttgttc ttttaatgca tgatacttat ggaaaacaag aaacagtaaa tgcattggac
      961 caaattataa catattttaa agacaatggt tatcaattta aaattttaat ataataaaaa
     1021 agcaagttta ttgaatgtac attttatact caatagactt gcttttactt tactctattt
     1081 tactagctcg atttttacaa attttttctt tcctatttga acaactaatt cactctcttt
     1141 tacaatagtg cttaaatcct ctactttttc accatttacc tttactccac cttgagaagc
     1201 taatcttcta acttcactct tactcttaac aatctcatta gaaactagta cttcgattaa
     1261 gtcaaatcca tcttccttaa cttgaattgt ttgtatatct tcaggtattt gacctttttg
     1321 gaatacagat ttaaatcttt cttcagcttc tttagcagat tcttctccat ggtataatgt
     1381 tactatttct cttgctaagt tcatctttat atctcttgga tttactgaac cctctttcaa
     1441 ctgtgattct attttattaa cttcatcagg atgaacatct gtcactaaat tgtagtattt
     1501 aattataagt tcatcaggaa tttccataga tttttggtac attattcccg cttcttcatc
     1561 tattccaata tagttaccta aactcttact cattttttct ttaccatcta atccttctat
     1621 taaaggcatc attattacta tttgagactc cataccaaat tctctttgta atgatcttcc
     1681 cattagtaaa ttgaatcttt ggtcagttcc accaagttct atatcagctt ctaatgctat
     1741 agagtcatat gcttgcatta atgggtagaa gaactcatgt actgatattg gcatttgtcc
     1801 ttcatatctc tttttaaaat cttctctttc taacattctc gcaacagtaa tagttgctgc
     1861 taattttatt acatcttcaa aatttaactt agctaaccac tcgctattaa atcttacaat
     1921 agttttttct ttatctaaaa ctttgaaaat ttgttcttca taagtttttg catttgcaag
     1981 aacttgctct gttgttaaag cttttctagc tttagatttt ccagttgggt ctccaatttt
     2041 tcctgtaaaa tctccaatta ttattactat ttgatgacct aaatcttgta attgtttcat
     2101 ctttctaaga actactgtat gccctaaatg tatatctgga gcacttgggt ctagacccaa
     2161 ttttactatc ataggctttc cttctttttc tgatttaata agcttttccc ttaactcttt
     2221 ctcatctatt aaatcatcta cacctttcat aattattcgc atttgctcgt ctatcgattt
     2281 catttttcat tcctcctaaa atatttatat aatctataat ttttattttt ataataaaaa
     2341 aagctttcat cctcttaaaa ggacgaaagc attgcttacg tgttaccacc ttagttcatc
     2401 aatatctcac gatattaatc tcttagagta caattacata tactcgtgta ctataacgtg
     2461 tactaatccg atatctctta atggttgtta tctttcagag atagtgctct gagatgtatt
     2521 caaactaaat caatttattc catttcacca aactggaact ctctgttaaa taaaatctag
     2581 ccctacttct tctcttcata gcatttatat attcagatta tttcatcaat tacaagaaat
     2641 ttttcttaac ttactcataa tatattatta tttttatatt ttgtcaacct tttttattat
     2701 tatatacatg tttttttaca ttatactact tttttatatt ttttaccaat acccaaaatt
     2761 tatttttcaa tcaaaagatt tatttgttta ttgactatat tactctgact caatcttata
     2821 tttattttct tatttaaaaa tgcgtttata attattaaac caataactgt tacatcaaat
     2881 ataaaaaaca gcaataaaat tattatcaaa aataaaattc tagcatataa taattttcta
     2941 aacttagcta ttataaagtt ttgtagtgac atacatgagt ctctaatgat aaaatagaga
     3001 cttatatagg ttattgatat aaagattgtt tttaaataaa cagaatcaac tttaacattc
     3061 aacagtttta gaacttcaaa aatggctaga taagaaacac atatgtaaaa agcttttttt
     3121 gagcaacata aaaattggtt taaacttcta aaattcttaa atattaaaaa tttttttaga
     3181 gaattagaat ctaaaatatg caatttattc cctaaaatta aacttaataa atatatgcaa
     3241 aacaccatac caaaaggaaa caatgctatc attaaataat aaataaaatt tgtataccca
     3301 ttaaaataaa tccatttgct atatccttga aattctttca taaaactata acctattaat
     3361 gttgaagcat atatatcaat aaaaaccatt acaataagcc caaatactga accatatact
     3421 aaatcatcca taaccattgt aggcttattt attagatagc cacacattat accaagtaat
     3481 acactctgac ttgcccaaat agcagttcct agatttccaa gtaccagaga aattattaaa
     3541 agcgaagtaa ttccagataa aatggtatac tttaaatcac atttcaagca gattatacaa
     3601 aaaaatactg gcactataaa atctaagtaa atagcatatc caaatccagt actcacagct
     3661 ataattgtta ctactatgaa taaagatgac aataaagctg cttcagttaa ttttttagta
     3721 gtattcaaaa cactgcctcc ataaatcata aattttctaa ctaaagtttt gtttttaata
     3781 tagtatatca ctttttaaaa atatttttaa taaatataaa ttttaataaa ggtatttatg
     3841 ttaaaaatgg tataatgtat cttaatattc agttttttaa gatgctagga ggtaattact
     3901 atgtttaaaa ttaaacactt taatgatttg agtttagatg aattttatga aatagcaaaa
     3961 tcaagatatg aggtatttgc ttgtgaacag aagatatttt cattaaacga ttatgatgat
     4021 atagataaaa actcttatca catattttta aaagaaaatg gattgatttg tgcttacgct
     4081 agaataattc ctaaagaata cagctcttat aatgatgttt ccattggtag agttttagtt
     4141 ctttctagtc atagaagaaa aggattagca aaacaaatga tggattgtgc tatagatttt
     4201 ataaaagtta acttacacga aaataatata accttatcag cacaaactta tataaagaac
     4261 ctttacttat cttgtggttt taaagaaatc tcagaagtat acgacgaagc aggtattgaa
     4321 catattaaaa tgagattata gtgttgacaa gctaatattg ttaatatata atttaagtat
     4381 aaattaaata ttgaattgat atttaggtat ttgaaaatac cttacataga gtcagaaggc
     4441 ttttgctttc tgactttttt attatcaaaa ttcaataatt atatggattc aaagtgttaa
     4501 tttttattct atattctact ttccccaaaa gtaagaatta tacccaactt tgtatgatag
     4561 gcatcttgcc tatctttttt ttgtaaaagt aacaaggtaa taatataatg catagtttaa
     4621 aataagaaga ttagcccagg ctaaatttca agcatggagg tgctattatg aaaaacctta
     4681 acgatattca agtgaaaaaa actgtcaaag ttgaagatat attatctagt ggtaatttaa
     4741 gagaaagaat gttagcgctt ggtcttacta gaggagctgt tatagatgta gttagaaaag
     4801 gtccaaaaaa taatttaact gtctataaaa tcagaggtag caagatagct ctacgacaag
     4861 aagaaagtag cttaatatta gtttctgaca tttaaaaatt tctagcagga ggatattaat
     4921 atggggttga cacataactc aactaaaatg agttctttaa aagacatgtt tgatatagac
     4981 aataaggaag accagtttgt aattgcattg gctggcaatc caaatacagg taaaagtact
     5041 gtatttaatc atcttacagg acttagacag catactggaa actggccagg aaaaactgta
     5101 gctacagctc gtggtaattt taaatataaa aataccgaat atgcattaat agatttacca
     5161 ggaacatatt ctttatttgc cttatcacaa gaagaaattg tagctcgtga ttttatatgt
     5221 tttggtaatc cagatgcagt aatagtagta tgtgatgcta catgtcttga aagaaattta
     5281 aatttagtat ttcaagtgat ggaacttaca gataaagtta ttttgtgtat taatttaata
     5341 gatgaagcta gaaaaaaagg cattactatt gataaaaaac ttttagagga ttctcttggt
     5401 attccagtta ttttaactgc tgcaagaaat ggctctggta tggatgaact attggatact
     5461 ttaaatgatg tatcttttga caaatataaa ttaaataata aacctgttag atacaatgaa
     5521 aatatagaga atgtggtaaa atcaattcaa ccagaattgg ataatattat accaggtata
     5581 aattctagat ggttgggact tagacttatt gatggcgatg aaagtatatt tgaatctatg
     5641 agtaattata tagacaaaaa ttctatagat gctattaatg aagtaaagaa aaagattcca
     5701 gacaatataa ataaacagaa aataagagat gagtttacta aaatcaacta tgattatgct
     5761 aaaaaactga gtgatgagtg ttgttctaat gttgctaaaa aatcaactga tagagaagaa
     5821 aaagtagata aaatattaac atctaaaata tttgggcttc caataatgct cttattatta
     5881 ggtactatat tgtggattac tatagaagga gctaactatc catccacttt attatctaat
     5941 ttattactcg gatttgagcc aagtatttcg ggtatattaa atagtattaa ttgtccatca
     6001 aggttaaatg atatgttggt tttaggatta tacagaacat tagcatgggt aatctctgta
     6061 atgctacctc ctatggcaat attttttcca ttatttaccc tacttgagga ttttggatat
     6121 ttgccaagag ttgcatttaa cttagaccac ctatttaaaa aagcttgtgc acatggaaaa
     6181 cagtgtttga ctatgtgtat gggatttgga tgtaatgcag ctggagttat tggttgtagg
     6241 ataattgact ctccaaggga aagacttata gctatactta caaacaactt tgttccttgt
     6301 aatggtcgtt ttcctacttt gatagctata tctacaatat tttttagctc agttataacc
     6361 aactctttcg tatcaagcgt agcaactgca ctttgcataa ctttactgat aattctaggt
     6421 gttataataa cattgttggt ttcttacact ctttctaaga cgctattaaa aggagttccg
     6481 tctacattta cattagaact tcctccttat agagttcctc aaataggaag aactttatat
     6541 acctctataa tagatagaac aatatttgtt ttaggaaggg ctgtaatggt agccatacct
     6601 gctggtgtca taacttggat atttgcaaat atatatatag gagatttaag tatattatca
     6661 catgttgcaa actttttaga ccctttagct aaattaattg gtcttgatgg ttttatatta
     6721 cttgctttta ttcttggatt cccagcaaat gagatagttg tacctatact tttaatggct
     6781 tacctagcaa ctggttctat gatagaatta gatagcttta gtgctttagg tcaagttctt
     6841 agagaacatg gatggactta tttgacagct ttaaatgtta tgctgttcag tttactgcat
     6901 tggccttgtg caacaacact tttaacaata aaaaaagaaa ctggtagttt aaaatggact
     6961 gccttaggat tcttaatgcc tactatactt gcttttgttg tttgtttttt aacaactaca
     7021 gtttacaatt tatttatata attagtatta actgacagta gacatatatt agagaaacca
     7081 tttaaaaatt tatatacttt attataaagc atagagatat attcaatgat aagtaaaatt
     7141 acttaacatt tgaatatatc tctattttaa ttcttatctg gatacttgga ttgaaatttc
     7201 agtcatataa ttatcttcat tactttcaca aaaaaagtca agtatactct cctcatacga
     7261 atttccaaca attttatatt cattttcata tatatatttt ttcaattttt cgtaagattt
     7321 atatgaatcc tcatattttc caatatgttt tatgcaagca tacattcctt ctggctttac
     7381 atatagcttt ttatccttta tttttttatc tatcttcaaa taaaaacttg atatacttaa
     7441 atagtcccca ttttttatat tttcattact tactattact cccaactcat atccttgata
     7501 caaatttttc gatttacaat aattggtaaa atctattaat tccatcataa aatcactatt
     7561 actcatatta ctcaaatcgc ttgatagaac taaatattct tcaaactttt tggatatata
     7621 aatttcttca ctattcattt tagaaattcc ttcaactgtt atatttattc tattttgcat
     7681 cttagaactt atctttctta gtttttttat tttttctctt atcttttttt cttcttcttt
     7741 aagcattttc acacaaagct ggggagttct attttgtata tactctttta tttcctctaa
     7801 actcattcct atttctctta atgattgtaa tatatcaagt gtatcatatt gataaactga
     7861 gtaatatcta taattatttt tatctttata ttcaggagaa aaaataccta ttttgtcata
     7921 gaaaattaat gtctgcttag atataccaca caatttagca aattcacctg ttgtaaaata
     7981 ctgtttttcc aagggttata cactcctttt ctttttgttg tctatatagt tactatattc
     8041 tttattattt actaaatcaa gtagttatta ttttagaaaa tcaatatggt tttgatttta
     8101 aagcttaaat atcagttaga tgtcagttat aaatatttta ccctttccca tcataaaaaa
     8161 gatatcccct cttaactttt ggatggaata ttatatttat acttgttaga gtgaaaattg
     8221 taataggtgc tacaataaga tatttaatta aacataaagt taattattgt aataaaaaat
     8281 ctttagataa attagagtta aagtacggaa atatagatag agaaaaagct gttaaactag
     8341 aaatatttta tcagtattta atagaacttg aatatataga aatgagttta ttaataagaa
     8401 gattagatac tgcaataaca tcattagcat tagtatctat tataacaata gcatcatatt
     8461 atttaatcag aaaaaatgtg taattatgta agctatttat aataaaacac tgataaaatt
     8521 aataataaaa aggtaatagg atttctatac tacctttttg tttttataat atagtgtaat
     8581 tttattatat ttttgtatgc taatgtaact agataatcat aagataacac attaaaagaa
     8641 aattatgaaa acttacattc tgtatagtta atataaaaca taagtaatat aaatttatgt
     8701 aaagcaacaa aagatagatt tacattccat atagttaata taaaacctta gagaaagcga
     8761 aaacgtacca ttaaaatcaa atatttacat tccatatagt taatataaaa cgttgtaatc
     8821 atagttccaa ttagccactt taacatgcat ttacattcca tatagttaat ataaaacacc
     8881 aaaataaact tagtattttc aacacttaca cgtatacaac tctcctaaat ttgcagtgaa
     8941 cgagcaatag tgcaattgat aacgcttatc atgcactctc aacgccttat atttcaactg
     9001 ttacgccaaa ttttttcaca aatatcgctc actgcaaaat tcctataatt ttattatatc
     9061 ataaatatat tacttttgaa tattgatacc aatttatacc ataataaaag caaaaaaacg
     9121 taatctatct acattagatt taaattctat aacttaaagt tttttacata atatttatgc
     9181 ttgtttaatc aatctaatat atcattcttg tatattgctt ttaatagtct tttttcttaa
     9241 ataaatatct atctgaacaa gcatagttta attaaaaact tttgtattcc cttaaaactg
     9301 tatctacctc ttattatttt atttatgata tattattttt aatctaaaaa tagatatcta
     9361 tttttattat ggttatccct ttttaaaata ctttaatcat atactattcc caatttaaca
     9421 tccattgaaa tgctattttc ttatattgca atttataatt gatatatctt caatataata
     9481 atttaatgta tttttgattt taaagctttt cagtgatatt attgataaat tagaattaat
     9541 tatctagctt taaaaggtca aagatttctg ttgctgtctg cggttttcca aataaatatc
     9601 cttgtatgta atctggcttt atttgtttaa ctaaatcata ctcttcttgt gtttctacac
     9661 cttctaaaca tacttttatt cctacatcat gacaaattgc aacaataaaa tgtataaatg
     9721 tagcatcaaa cttactcttt aatatatctt taacaaatac tctatcaatt ttcactatat
     9781 ctattggaga aaattttaaa acttctagag acgagtatcc agtaccaaaa tcatccatag
     9841 caatataaat tcctaaatct tgtaaagcct taaatttaaa ctgtaaaata ctcatatttt
     9901 gtactgtatg actttctgtc aattctaaaa caagattttg ataaggaaaa ttttcatcat
     9961 ctataatttt tactatatct tcaataaatg tgtcttctaa tatttgaaca gctgaaacgt
    10021 ttacactcac tttaaaaaaa ggatagtaat caatccattt cctaaaagta cgtaatgcca
    10081 ttcgaagcac ccaagcccct acaatattta tcatatcatt ttcttctaat attggaataa
    10141 attccaaagg agaaattgct ttgcatttgt cattagtcca ccttaaaaga acttctacac
    10201 ctattatttt atgactttct gtatctactt gtggctggaa tcgaagacta aaccccctaa
    10261 aatcatcatt aattgatgcc tttaaatccc gcatcatttc taatgaatac attttatttt
    10321 ttaatatttc ttgggagaaa aaaacaagcc tgtttttacc atgctctttc gcatattgta
    10381 aagagtaact agcacactta taaagttctt ttactgtatc tccatgtttt ggatatatta
    10441 cacaaccagc agaaattgtt atatttaatc catattttct ccatagttga aggtgtataa
    10501 tcatattctg tatttgatta tatagagtta gaatttcatt ttcttcaaca ttatcaacaa
    10561 gaatgcccat ttcgtcccca tcaagtttat aaagttcagc attatctggt aaaatagatt
    10621 gtacagactg tgctagtgtt tttattatat tatctccaaa atccctatca tacatctcat
    10681 taacattttt aaaatcgtca atatctaata atactatgca taatttttca atcataggat
    10741 ttgaaatttt atcttcaaat acactcataa attcatgata attcagtaac tgtgtcaatg
    10801 aatcaatctt attttgttta cctaattgcg tcattattcc tgcaaaaata cttggctctc
    10861 caaattcatc acgcattagt tgacctctac atcttaacca tatatattct ccactacgat
    10921 ttttagccct aaattccact gtatgataat ccatctggtt tttacctatt tcagtgtttg
    10981 acttataaaa acgattccag tcttctggat gtacaatttt tttccagtaa acaagcggat
    11041 ttttaacaat ttctcctggt aagtcaaata attcaacttg tgatggtgaa tatcgaaata
    11101 cgcctgtttt catattgcaa atgtatatat attcatctgt acctctaatc aaagcatcgt
    11161 aaagcaatgt agaattataa tcaaaattgt ctcttgaaaa attaaaaact cctttttctt
    11221 ctttattatt tgatatttca tgaaattcat taatcttaat tggtttattt aagcttgttg
    11281 agtttgcaat atctattgat tgatgcatat gaacaactgt gccatcttgc catgtaatta
    11341 aactatcata attttcaaaa acacgattta acttattaca tttttcatac cactttatta
    11401 atactccttt tttatcattc tttaataatt ctaaaacttt acaaaaacta caagtagaat
    11461 ttttttcagg atataatact tgccaacaaa cttttccttc tggatctaaa atattatatt
    11521 cttctttcat ttttttgttc ataaaaataa tttcattagt atgaatattt gtaacatata
    11581 tattaatctg tagttgattt aatataactt caaaattatg tttgttcata tacttatctc
    11641 ctccatagtt actgctcttt ataacaacat tgttaaaatt attcttttta gaagatttac
    11701 ttataactta tacatataca ctaaatacct taatttaatt agaaagttta ttacttcata
    11761 ttttaaatta taattgataa aaaaaaatac agcaatatag tattaccaat ttaaaaaaaa
    11821 ttaatattac ataatacatc tctctcttat attaaatttt aacttggtac taatcttgac
    11881 aaattgtcca caaaaataaa ttatatattt tattattgtt ttaaattttt ataaaaaatc
    11941 agtattattg atactattga agttaaaaag cattaatttc taaaaacatc ataatt
//
LOCUS       DAELTA010000111        11815 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.111, whole genome shotgun sequence.
ACCESSION   DAELTA010000111 DAELTA010000000
VERSION     DAELTA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.111"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            180..809
                     /locus_tag="KPG13_002607"
     CDS             180..809
                     /locus_tag="KPG13_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986310.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            810..1592
                     /locus_tag="KPG13_002608"
     CDS             810..1592
                     /locus_tag="KPG13_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986311.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1585..2463
                     /locus_tag="KPG13_002609"
     CDS             1585..2463
                     /locus_tag="KPG13_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986312.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2496..3455
                     /locus_tag="KPG13_002610"
     CDS             2496..3455
                     /locus_tag="KPG13_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0986313.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3770..4462
                     /locus_tag="KPG13_002611"
     CDS             3770..4462
                     /locus_tag="KPG13_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986314.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4472..5476
                     /locus_tag="KPG13_002612"
     CDS             4472..5476
                     /locus_tag="KPG13_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986315.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5744..6595
                     /locus_tag="KPG13_002613"
     CDS             5744..6595
                     /locus_tag="KPG13_002613"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG0986316.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6699..7445
                     /locus_tag="KPG13_002614"
     CDS             6699..7445
                     /locus_tag="KPG13_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986317.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7445..9361
                     /locus_tag="KPG13_002615"
     CDS             7445..9361
                     /locus_tag="KPG13_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986318.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            9641..9874
                     /locus_tag="KPG13_002616"
     CDS             9641..9874
                     /locus_tag="KPG13_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG0986319.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            10070..10498
                     /locus_tag="KPG13_002617"
     CDS             10070..10498
                     /locus_tag="KPG13_002617"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG0986320.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(10952..11722)
                     /locus_tag="KPG13_002618"
     CDS             complement(10952..11722)
                     /locus_tag="KPG13_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986321.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
BASE COUNT         4650 a         1132 c         1950 g         4083 t
ORIGIN      
        1 gatgtatgca ttattaatta aaataaatta catatactat ttaatatttc atttaaaata
       61 ttaaaaaatg tagataaaaa ttgttttatt aagaaaaaat taatatattg acaaaattta
      121 agtgctcatt taaactatta attgataata atattcaata ttaaataata ggaggcgtta
      181 tgaataaata tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc
      241 ttattaaagc aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag
      301 aatatgtaaa aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt
      361 gtagatgtcc agaaagtata agtcttttaa aaagaaataa tttgataaat gattttgaag
      421 tacctatgat agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa
      481 aaagtaaaga tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa
      541 gtgaaaagtt taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac
      601 aagaaggtgt aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca
      661 aagatacttt tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac
      721 aaaaaataag aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag
      781 atggttgtaa taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa
      841 ggctagtaaa gagtgttttg atatttttta taattctttt attatggaaa attacaaatt
      901 atcttggtat ttggagtgat tatatattac catcacctga aaaggtatat agtacatttt
      961 taaatatgat aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt
     1021 tgataggttt tgcaataagt actgctatag gtgtgccttt agggatattc tttggaattt
     1081 atagtggagt atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc
     1141 ttgctttaat accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa
     1201 ttatagtttt agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt
     1261 gtgactcaaa actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat
     1321 ttaaaataat aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg
     1381 gatatagctt tagagccatt attggggcag aacttgtagc agcttcatct ggtcttggat
     1441 acttgatatc tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg
     1501 taattggact tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa
     1561 gtaagggaaa gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt
     1621 agaaatttat ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc
     1681 tctttaaaca tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag
     1741 actaccttgc ttagagcaat tgcatcactt gaaggtataa atagtggtga aattaggttt
     1801 attaatgata ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta
     1861 atgccttggt taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt
     1921 ttaaacaagg tgcttagaaa atttaaacac aataaaagaa ttagcagtaa aattataaat
     1981 gataatttta gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg
     2041 aaaagtaata ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac
     2101 tcttatccaa atgaattgtc aggaggtatg gcgcagagag taagcatagc tagagctctt
     2161 tctttcaatc cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca
     2221 aggattgata tgcaaaatga agttataaga atacataagt caactaataa aggcgtgata
     2281 tttgtaactc atgatattga tgaagcgtta aagataggca agaaaatagt tgttttcaca
     2341 gacgaaagaa aagtaaaaga atttaatata gaagatggtt ataatagaga tttaacttca
     2401 gattactata ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaaggcatt
     2461 taaagaaatt aataatattt taaggagtaa agtaaatgaa attaaaaaag ctattgatag
     2521 gtataacaat attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg
     2581 gagaaaaact ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta
     2641 taattcaaaa acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta
     2701 atttccacga aattactaca ggtccagagc aaacacaagc tttagctgct ggagaaatag
     2761 acttcttaca tgcactaggt ggtacatcag ctttaatagc tgcatctaat ggagtagagt
     2821 taaagatatt aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata
     2881 attcaataaa atcagcagca gatttagtag gtaagaaggt ggcaggccca aaagggacta
     2941 tattacatca ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg
     3001 aatttgttaa tatgggtatt ccagaggcat cagcagctct ttctgatggc agcgtagatg
     3061 ctgcattgat tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag
     3121 caaatggtga aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag
     3181 agaaacatcc agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg
     3241 taaataataa ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg
     3301 aagaaactaa agaattatat gcttggtatg attttagttt agatataaca gataaagaca
     3361 tatcttcgct tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag
     3421 tgaacataaa agaattaatc tataatgcta agtagaaact atgataaaat aatactaagt
     3481 aagaaatata gtaaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag
     3541 taatttcaat ttattttaga atgattagtt gataataatg attaattaat cataaaaaga
     3601 tactttttta tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt
     3661 aattgtgaag ttagtaatat gattaaattt atatattgta aaaagattat aatatattaa
     3721 aattggactg ttaatatgat gagtaaatag gtatattagg aggaattata tgagttataa
     3781 agtatatatt gtggaagatg atatatcaat aagtacatta cttaaagatt atattgagaa
     3841 atatggattt gaagtgattg ttgaagaaaa ctttgatgat ataatgatta catttgaaag
     3901 ttttaagcca cacttagttc tattagatgt aaatttacct aaatttgatg gtttctactg
     3961 gtgtagatgt attagacaac agtcgaattt acctataatt tttatatcag ctagagatgg
     4021 taatgtagac caaattagag ctttagaaag tggagcagat gattatataa caaaaccttt
     4081 ttactatgat gtagtaatgg caaaaataaa gagtcatata agaagggtat atggtgaata
     4141 ttcacctaaa atagacgaaa gaatagtgga gctagatgga ttaaagtttt atccagagag
     4201 gttagaacta gaattaaatg gtgagaatct aattataact aaaaaagaag gtatactgat
     4261 tgaatgtctt atcaagagat acccaaaagt agtaagtaga gattatcttt tagaaaagat
     4321 atgggatgat attgagtttg tagaggaaaa tactttaaat gtaaatgtta gcaggataag
     4381 gaaaagattt gaggaattag gtatagataa tgctgttcag actgtccgag ggttaggata
     4441 taaattaaat aagaattggt aaggagaaag tatgaagtta tttttaaaag attataaagg
     4501 ttatattttt atatattata taggcatagt tatgacaatt atttactgta atcttatgaa
     4561 gtttattagt tttggagaga gtttatatat tcttttattt agttctttta tattgttatg
     4621 ttttttaata tataagtatt ataaaaatag agaagtatat aatctgtttg aaaaaggagt
     4681 acattctttg gaggagtcat ttttgtattt aggaagctca ttcttgggga gaaatatatc
     4741 agttatttta aaacaacaac ataaattgta tcaatcaatg atacaagagc ataaaaagat
     4801 acatagtgaa catttaactt tcatcaacca atggattcat cagatgaaaa caccaatatc
     4861 agttataagc cttcagcttc aagattatga gggagaagaa gtaactgaaa atataagaaa
     4921 agaaatagat agattaaata aagggttaga tatggctatg cattttgcaa gggcagataa
     4981 ttttcagaaa gactttatag ttgaaaaagc caaattaaat gaaattgtat ctagtattgt
     5041 aaatgaagaa aaaaggttat ttataaaaag tggaatgatt cctaagattc aagtagacaa
     5101 atttacttat gtttatacag atgtaaaatg gatgaaattt ataataggtc aattaattac
     5161 aaatggagtg aaatattcta aagattatag taactaccta actataaaat ctgaagaaaa
     5221 taataaatat gtaattttaa gtataataga tgaaggaatt ggaattgttt ctaaagattt
     5281 aaaaagggta tttgacccat tttttacagg agaaaatggt cgtaagtttg gagaatctac
     5341 aggtatgggt ctttatctag taaaaaaggt atgcgatgat ttaggtcata aagtaagtat
     5401 aaaatcagaa gtaggaaaag gtacagaagt ttcaatctac tttaataaag atgtaaatag
     5461 tttaataaag gtgtaaatag tttgataaag atgtaaatta tctgttacaa tagggttttt
     5521 agttaattgt acttttttat tatacaattt attaaatgaa atattcatag ttaaataatc
     5581 aaaatttaag agcgtttatt tgcaaacctt acatagatgt aaggtttgtg taatgtttgc
     5641 agatagttaa attttctaat ctaaattaca attaatataa gaaataacat aagagaaata
     5701 acataaaaaa agaaattttg taaaaaagga aggatgttta ttgatgagtt tagatgttat
     5761 ttttatattg aaatcagtca taatagctat agtagagggg cttacagaat ttattccagt
     5821 ttcatctaca ggtcatatga ttttggttgg taatttaata gattttaagg ggcaatttgc
     5881 tgaaatgttt gaggttgtaa tacaacttgg ggctatatta gcagtagtag tactttattg
     5941 gaaaaagata aaagatagcg ttatagaatt ttttaaattt atatttactg gtggaaaaga
     6001 aggaaagatt ggttttagat ttggaatgaa tgtaattata ggttgtatac catttgcgat
     6061 tataggagtt ctattttacg ataatataaa atcattgttt aatttacaat ctgtaattat
     6121 aggatttata gtgggtggga ttttattatt agtagtagaa actttgttta gaaaaaagaa
     6181 tcattccaca gataatatag ataaaattac tccaatacaa gctttaaagg ttgggacatt
     6241 acaagttttg tctgcatggc caggtatgtc aaggagtgca tctactatta tgggtggatg
     6301 gatagcagga cttaattctc ctacagcagc tgagttttct ttctttttag cagtaccagc
     6361 tatggtggct tcttcaggaa aagatttatt tgaatttgat tattcaataa tgacaccaac
     6421 attgtggatt gctttggtag ttgggtttat agtagctttt attgtatcaa taatagttat
     6481 ggaaaaattc gtgaattttt taaagaaaaa accaatgaga gtgtttgctg tatacagaat
     6541 aataatgggt gtagtacttg cagtattagc ttttactaac ataattagtg tatagaaaaa
     6601 atagatttat catctataaa aattaaacta agataaatta taattaacaa ttatatatat
     6661 gttatttcaa aaattaaatt aggaaggata gttaaataat gaaaatttta actgtaaata
     6721 atttaagtaa agtatatggt aaaaaaataa ttttcaacgc attaaatgac ataaatttta
     6781 gtatagaaga tggagaattt gtaggaatta tgggtccatc tggtagtgga aaaactactc
     6841 ttttaaatat gatttctact attgacaagc caactacagg aactatggaa ttaaagggca
     6901 agaatccttt attgttaaga ggtgaggaac ttgcattact tagaagaaga gagttaggtt
     6961 ttgtatttca ggattttaat ttattggata cattaactat aggagaaaat atagtattgc
     7021 cattgacatt agataaggtc agtgtaaaag agcaagatga gagattgaat gaagtatcta
     7081 caatattagg tataaaagat ttattaggaa aaagaacttt tgaagtatca ggtggtcaag
     7141 ctcagaggac tgctattgct agagcattga ttaacaatcc atcaatactt ttagcagatg
     7201 aaccaacagg taatttagat tcaaagtcat caaaggttgt aatggagcta tttcaaaaga
     7261 taaataaaga aaataaagtt actactatga tggtaactca tgacccatta gcagcgagct
     7321 actgtagtag aatcttattc ataaaagatg ggtctatata taatgaaata tataagggta
     7381 gtagtagaga gcagttttat caagagataa tggatgtact tacattattg ggaggcgata
     7441 actaatgaat ataaaacagt ttgctattaa caatatttca agaaatatta aagcgtattt
     7501 aggatactta gcaagtattg ttatatcatc ttcactgcta ttttctttta ttatgtttac
     7561 aagtcatcca gatttagatg tatctcaatt tcctgattat ttaaaagaag gtctcaagat
     7621 gagtaagata attgcatatc tattcttatt cttctttgtg ttttattcag ttagtgtgtt
     7681 tcttaaaagt agatataaag aatttggaat actttatatt acaggtatat caaagcgcca
     7741 agtaatgaaa ttaatcttta tagaaaatat gataataaat atagtatctt ctgtaattgg
     7801 aataataata ggcttaatct ttgcaaagat atttttagtg attatgtcta cttttttaga
     7861 attatcacca ttaaaattct atataccatt taatgcgata ttgtctactt taatctattt
     7921 tatgatttta gctgtattaa catcaatatt tacttctttt atagttaaag aaaatcaggt
     7981 gttgaagtta cttaaaggta caaaaactcc aaaaccagag cctaaaacat cactaatgct
     8041 tgctatatta agtatttgtt tgtttataat agcgtattat aatgctatta cctcaaccga
     8101 ccaatataca acatatctgc gcttagttcc agttacatcc cttactatag tagcaactta
     8161 cttgttcttt tctcaattta gtgtattttt tatgaagaag ctaaaactaa acaagagatt
     8221 ttatagaaaa aatacaaata tgctttggat ttcgaacttg atttataagg taaaggataa
     8281 tgctagaata tttttcttga taactataac ttctgcagta gcatttactg ctataggtac
     8341 agtttattcc ttttggaaag atgtagagcg tcaaattaat ttaatatatc ctaacacaat
     8401 atattattca acaatgacat tgcataatga ccctaaaaag cctgatagca aagaaaaaga
     8461 caaggaaaga atgatgttta tagaaaataa tctaaaaaaa gagaatgtag actatacaag
     8521 gactaatggt gaatttaaaa ctgtatttcc caagaaaagt gattttaatg tcagaattat
     8581 gaaagaatct aagtacttag aaattacaga aactataggg gttaagtcta taagttttga
     8641 agataatgaa tgtatatcat tattatcatc aagtttacca ggaaataata acgtcaaaga
     8701 aaatattatt gtagggaata ggggtttaaa ggtggcaaaa caagtggaag aatgtgttat
     8761 gccagcatat tacaaaaata tgtatgtggt aaaggataat ttttatgata gtatagaatc
     8821 aaaatttata gtggatagat ttactgcatt tgaagtagag gattcaagta aaactataga
     8881 gatatgcaga gaatttgaaa ataaatttaa taatgagtca gggatgcaac catatttgtt
     8941 tttctctaaa gaattaatga taggaactgg gaaattaatg tattctacat ttatattttt
     9001 agcaatattt ataggtttaa ttttctttgt tactacaagt agttttttat ataataaatt
     9061 ttatatggat tgccaagttg ataagaaaaa gtatgaacag ttaaataaat tgggtatgac
     9121 atataatgag attaaaaaag cttcaacaat tgagattgga atagtatttt tgtttcctta
     9181 tgtagtggct gttatacatt caatatttgc acttactgca cttaaaaatt cttttgatat
     9241 agaagtagca tcatcagctg ttttggtaat gggaagcttg ttcttggttc aaatagttta
     9301 tttttttatt gtaagaaata attatttaaa agagataagg ttaaatttag tcaattttta
     9361 gttactatta ttaaatgata ataaaaaggg tttaaaatta attaatatta ctggaattat
     9421 agatgtttat tttttcagta atatttttta ttgtattaac tattaaaatt ttgtacaaat
     9481 ataaaatata atatggtaaa tgtcaaaatt ataaaaaaat tctacttata taaaatataa
     9541 agaggttata tgtacatatg tgtagaaata aataataaaa atatcaatta atgttattag
     9601 gggggggtta ctcattagta aattagctaa tacacttaga atgatgatat taattcaaag
     9661 cagaggcaaa atgaaatgca aagaattatc agaagagttg gaagtaagtg aaaggcaaat
     9721 aaaaagttac aagatgtatt tagagcaagc tggtatattt ataaattcaa cacctgggat
     9781 ttatggtggt tatgaaatag ataaatgtaa tagcatatca ttaattaaat tattagatag
     9841 tgaagtttca atttttatat caactaagtg gtagataagt agaatattag caatatcaac
     9901 gagtatttag aaacttattt tatattaata aagcagtata taaaattgta gaatcaacaa
     9961 tagcatatac taaaacatcc accttatatt aactaaatca tatctaaata aaatacactt
    10021 acctacaaac attataaaat caatacaaaa atgaggtgaa acaaaattta tgataaagaa
    10081 attaaacagt aaagacataa ataaaatcat ggaaatatgg gaaaaaagta caatcaaagc
    10141 acatgacttt ataagtaaag aatactggca aaataactat aatactgtta aaaacgaata
    10201 tatacctata tcagatacat ttgtatatga tgatggagat gaaataaaag gatttataag
    10261 cataatagat aaaaacttta taggagcttt atttataaag cccaaatacc aaaatctagg
    10321 tatcggaggt aaacttttag attatgcaac taaaaaatat aaaagtctaa gcttagcagt
    10381 atataaagat aataaaaaag cagttgtgtt ttataataaa aaaggtttta atatagtaaa
    10441 agaacaagta aatgaagatt caggatttaa agagtacata atggaatata gtaaataata
    10501 tgattacatt ctattaaaag gataattaat ttattttcta gttgattttt taagagtaat
    10561 ttttaaaata gactatatga agataaagat atttattata gatgtaagtt aatcagaatt
    10621 gatatagcta caaaagagga aaataaaatt gtatggtgta tttcagattt aacaaattta
    10681 ctatttaaag ataataagtt tgatataata ttaaagatac tttcacctat aaactataga
    10741 gtattgaaag aattttaaat aattatggaa tgctaataaa ggtttgttaa aatttaataa
    10801 ttattagatt atagaaaatt gccatgaaat tagttcatca agaactagtt tcatagcaat
    10861 tttaattctt ataagtgtat ttatttttac agatttaaaa aacttataca gtatctaata
    10921 atgtaatctt attactttag cctataaata tttaaatctt aaaatgttct ctttgagata
    10981 agcttatttc agagtcacaa tttccacaac agtaaaatgt tgagacatct ttaaattttt
    11041 cagtattctc atcaaagttc atttttttct ttgcaataat tcctacctta gttaaattag
    11101 aaccacaatt attacagttt tgaattttgc ataggttata tagtttatca agtgtaatat
    11161 cttctaataa ttcatcacaa ttacaacatc tgatagaaga aacttcacta cacatttcaa
    11221 taggttcagc ttctttagaa gaaaatttat atgttatgtc atttgatact ttataacctg
    11281 tatcaaatat attagaatta caacttggac ataagtactt tggaagatat atatcttttt
    11341 tttcaaaagt atcactacta atattacagt ttaattcatc taaaattggt ctttcatcta
    11401 ggttattacc tttatttaag cttagaagat aattataaat ttttttatct tcattctgtt
    11461 ttgatgtatc taacaaatgc tccattatat tagtgtattt tttgtttttt gaaggcatgt
    11521 ttaaatccag ataattgttt tcaatagcaa agcatactat attgaaatag ttatatttaa
    11581 ctgcaaaaaa taataacatt tcatcaaata ctttatctat tattttgtca gaaatactta
    11641 tccaaaaatc tggtgactct aagtaaactt tcctactaag ttcatctaac tcttcatcta
    11701 tattattttc attaactttc atatcatcac ttccttttaa atatcttgtg tataaactat
    11761 ttgttactat tttttatata aaataaaatc ttatataaaa atatatcata ttata
//
LOCUS       DAELTA010000112        11760 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.112, whole genome shotgun sequence.
ACCESSION   DAELTA010000112 DAELTA010000000
VERSION     DAELTA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.112"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            97..987
                     /locus_tag="KPG13_002619"
     CDS             97..987
                     /locus_tag="KPG13_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986322.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1205..2959
                     /locus_tag="KPG13_002620"
     CDS             1205..2959
                     /locus_tag="KPG13_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG0986323.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            3343..4329
                     /locus_tag="KPG13_002621"
     CDS             3343..4329
                     /locus_tag="KPG13_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG0986324.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            4498..4646
                     /locus_tag="KPG13_002622"
                     /pseudo
     CDS             4498..4646
                     /locus_tag="KPG13_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            4665..6146
                     /locus_tag="KPG13_002623"
     CDS             4665..6146
                     /locus_tag="KPG13_002623"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG0986325.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            6172..7143
                     /locus_tag="KPG13_002624"
     CDS             6172..7143
                     /locus_tag="KPG13_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG0986326.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(7399..8001)
                     /locus_tag="KPG13_002625"
     CDS             complement(7399..8001)
                     /locus_tag="KPG13_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0986327.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            8282..9493
                     /locus_tag="KPG13_002626"
     CDS             8282..9493
                     /locus_tag="KPG13_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG0986328.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            9561..9758
                     /locus_tag="KPG13_002627"
     CDS             9561..9758
                     /locus_tag="KPG13_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG0986329.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            9792..10763
                     /locus_tag="KPG13_002628"
     CDS             9792..10763
                     /locus_tag="KPG13_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0986330.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            10950..11327
                     /locus_tag="KPG13_002629"
     CDS             10950..11327
                     /locus_tag="KPG13_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0986331.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         4824 a         1171 c         1904 g         3861 t
ORIGIN      
        1 ataaaagaaa taaaaatata ttataaaaac aatttgattg gaattttcag aaaattaatg
       61 ctataattaa aagtaaattt aaagggaggt tataaaatgg ctaaattaac tctagaacat
      121 ataaaaggaa atacatatta tattccactc cctacaatag taggtgtata cgtagatggt
      181 aaggatgcaa ttttaattga cagtggaaat aataaggata cagctagaca agttttaaga
      241 ttattagaag aacataattt aaatcctaaa ttaataataa acactcattc aaatgcagac
      301 catataggag gaaatgcata tttgaagaat caaacaaaat gcaagattgc tacgactaaa
      361 attgaaggat attttacaga aaatccaata ttagaatcag catttttata tggtggctat
      421 ccaagtaaag cactgaagaa taaattttta ttagcaaagg aaagtgaagt tgattatatt
      481 atcccttcaa atggcaaaat tgtagataca gaacttgaag cgatttctct tccaggtcat
      541 tattttgaaa tgattggtgt tagaactcca gataatgttt tgtttgttgc agatagtctt
      601 acaccagaga atataattac taaatatcat tttttcttcc tactggatat agattcacag
      661 tttaagacac tagaaaaatt gagaatgtta gaggctgatt tctttgttcc aagtcactct
      721 gtcaaaacta cagatataaa aagtttaatt aatattaata agaagaagat ggaagaaata
      781 atagataata ttaaaaaggt ttgttatgag ccagttatga ttgatgaagt tattgaaaaa
      841 atgtgtgatt tgtacaatgt caagcttgat gctaatcaat atgtgttggt tggaagtaca
      901 atacgttctt atataactta tttatatgag aataatatgg ttgaatatat atttgatggt
      961 ggtaagatga tgataaaagc tgtgtaaata gtaataattt tcaaattata tgatgaaatt
     1021 gttttagtat gactgttatt tgaaaagtaa tataaaaaaa tttatataaa taagctaaga
     1081 tgattgatat aaagttactg ataattttaa ggtaatatat acgttactat aatattatgt
     1141 tattatttgt aaataagggg ataatttata agcaaattaa agaccttaat ttacaggagg
     1201 aatagtgtat attatgaata aaaaattcaa aaaattcagt aaaaaactaa gtatattaat
     1261 tttagttttg attatgtttc tttttaataa tttaactgca aatgcacaga ttaaagaaga
     1321 ttcaataata ggaattgata gatatgaaac agcaggactt atagcagata gacaaactta
     1381 tgatactgta atattagtta atggagataa aaatttatct gatgggcttt catcaagtgg
     1441 attagctgga gctattaacg caccaatact tttgactaaa aaaagtgaaa taccaaaagc
     1501 tactttaaat agactagata ataagagttt aaatgttgta aaaaaagtat atataattgg
     1561 tggttataat actattgaaa attcagtaga aaaagatata aaaagaaaag gtattcaagt
     1621 ggaaagaata aatggtaaca atagaattga aactagttat aatgtagcta aaaaaataaa
     1681 tgaagtaagt agtgtaaaag aggtaatgct tacaaatgga tttgtaggag aggctgatgc
     1741 tatgagtata gcacctgtag ttgcaaaaaa taaaggagct ataatactta cagatgggaa
     1801 atcagttcca tttaagactg aaagtttaaa tgtttacgct ataggtggca aatctgcgat
     1861 aaatgaggat ttagtaaatg aaactaaagc aactagaatc ggaggaaatg atagatttga
     1921 gaccaataaa aaggtaatgg aaaagtttta taatggagcg acagattttt atataacaaa
     1981 tggatatcaa ttagtagatg cactaacatt atctccttta gccaaagaaa aaccaatcgt
     2041 tttagttgct gatggaagta ataaaggtat tttaaaagga gctaaaagta taacaaaagt
     2101 tggaggtatt gatgccaata tatataaaca gtgtttagat atgattaaat ataataatat
     2161 ggatatgact ccaaatattt taaaacactt aacttcagtt gaaggaaatg aagtttctga
     2221 aatacttata gaatcagata atttaggtgt ggttgttgaa aagacgaata ccaataaatt
     2281 tgagtttgat tacgtaagtg taactaatga aaaaaattgt actttttctg tatataaaga
     2341 aaattcttct aataatataa aaaatggaaa gctagttgta agtgctaaga aaaaaataga
     2401 taagaaagat agtgatttgc aagatatgaa tggcgacaat atgataaatg caaataaaga
     2461 taggatggta aatgtgataa aaattggagt tcccgataaa atgtattcta attttaaagt
     2521 agatgttgaa agtggttcag tagaacttta taatattaaa ggaggagcta ctgttgatgt
     2581 caatgacggg attgctaaca ttgttgataa caatgtaaca aatcctttca atataaatac
     2641 taatagtggg atttcaggtg taacagctga aactatatca tctgaaataa aatttagagc
     2701 aaacaatgga attgtaaatg taacagctac gaatataaat ggcaatataa gtttatttgg
     2761 aagcgataat aaaggtacct ttgatggaat atttaaatta aatctgaaaa aagaaccatc
     2821 aaatttacac ttgaagttaa ttggaaatgg gataaataaa ttgccagaag gttggtctaa
     2881 agattatatt ttaggaaatg gacaaccaat tattgaagtt aaaaataatg gcataaataa
     2941 tatatcaatt ggagaataaa aacatatgaa aataaagttt atatagtttt aaaaattata
     3001 gaattttaaa atatttatat atattcttta aataaaaaat tattttttaa gtgataatta
     3061 agtgtttatc gataaggcta tggttgtaaa aatcatagcc ttatttttat attataaata
     3121 aattagagta tttatcaatt catagtagat aaaattgaaa ttaaatataa caacaaaaaa
     3181 tgaaaattac aattaattta atgttaaaga ctcaattatt tggatgtgga atggatagta
     3241 caagtagtat ataaaaagta ttgactggac aaataactat attttatttt aaatatataa
     3301 tataatgtaa taaaatatat caaatgtaga aggtgataac ctatgaaaaa aataacaata
     3361 aatgatattg ctaacttggc aggagtatct aaaagtactg tatcaagata tctaaataat
     3421 aaagatatca gtgattctac taaagagaaa ataaagacaa taatagatga atatggatat
     3481 gagccgaatg catttgctca aagtttgaga gctaaaaaaa catactttat tggtattata
     3541 acaccgtgtt tagattcttt tgtaaaatct aaaattatga tggcaataga tgaagaactc
     3601 aaagaattaa aatatacatc actaattatt aatactaata gaaaaataag gtcagaaata
     3661 gatagtatat caaaattagc tagtctaaaa gttgatggaa taatactaat tggtaccgaa
     3721 ataactaaag aacataagaa tgaaattgaa aagttagata taccgatagt tgtagttgga
     3781 caaaaagttg atggtatcaa tagcatagtg aatgatgatt atggagcagg atataaaatg
     3841 ggtcaatata ttgctaataa aggttacaaa aacattgtat atttaggtgt tgatgaaagt
     3901 gatatatcag ttggtttaaa tagaaaaaat ggagtactta atgggttaaa agataaaggg
     3961 tatgatgcaa aagtatttta tactgacttt gaccaagaaa cgtctataca aagaagtgga
     4021 gaaatgctag aaagtgaaaa tccagatata ataatatgtg caactgataa tatagcaata
     4081 gcaacaatga aagaaatcaa taagagaggt aaaaatatac cacaagatat atctgtagct
     4141 ggatttgggg gatatgatgt attgtctatt ataagcccta aacttacaac aataaagttt
     4201 gaaaataaaa atgcaggtaa agtagcagca aatacgatag taaatttaat acaagaaaga
     4261 aaagaaccac tattaaagga gattaaattt gaattaatag aaggtgaaag tacaataaat
     4321 aaaaattaag atacacatta catatattag taatgtgtat ttttttacta aaaaaactat
     4381 aattctaaaa attaggaaaa cttttttgaa agaaatattg catctgaaat aaatttatgc
     4441 taatctttaa ttaaaccggt ttcgaaaccg gtttaattaa accgatagaa tagcaaaatg
     4501 agtgcaaaaa taactgattg taacaaagta tcaaagtaat tattagagta cataggaata
     4561 aacaaaatat aaaagatgta gcacattgtg taataagact aagaattgtt ttagatagca
     4621 ataataaagc aaatataaaa ttataaaaga aaaggaataa aaatatgtac aaagagccta
     4681 aatatagaac catattagaa tcgacaaaag aagaattaaa taaattaaga aatatatctt
     4741 taaatgataa atataaacca ctatttcata tacatccaca acatggacta cttaatgacc
     4801 caaatggatt agcatattat aatggtaagt atcatgtatt ttatcaatgg tatccatatg
     4861 atgcaactca tggaatgaag cattgggctt atgtaagttc tgatgatttt gtaaactgga
     4921 atagagaaga tgtagcatta ataccaatcg aaagttatga atctcatgga gcatattcag
     4981 gtaatgcgat agaggtagat ggaaaattgc atatgtatta tacagggaat ataaaatata
     5041 gtgcagaaga tagatatgca tatcaaaact tagctataat gaacaaggat ggtaaaataa
     5101 ctaaatatga aaataatcca atagtaagtg aaataccaaa aggatataca ggacatgtaa
     5161 gagacccaaa agtatttaaa agaaaggata aatactttat gttacttgga gcacaaacaa
     5221 gtgataaaaa aggagctata atagtatatg aatctaaaaa ctctatagat tggaatttta
     5281 aaggagaatt aaatgtaaaa aatatagatg aagattttgg ttacatgtgg gaatgtccag
     5341 attatattaa tatagatgaa aaagatatat taatattttc accacaagga gtagaaccta
     5401 aaggatttga ttatcaaaat atatataatg tagtatatgc tattggtaat atggatttag
     5461 ataatttaac atttgaaata gatacaatga aagaactgga aaaaggattt gatttctatg
     5521 ctccacaaac atttataaag gactcacaaa taatactatt tgcatgggct ggaatgggag
     5581 aagttttata ccctacagat aaaaacaaat gggcacattg tctaactgtt ccaagaaaat
     5641 taaatataaa aaataataag ctattgcaaa tgccagtaga tgaattaatt aaattaagat
     5701 atgatgaaac aagtgggcaa aatactataa agaataatat taatattata gaaaatgatg
     5761 aaaatgtata tgagcttaat attaatataa aaaatattga cagcaataaa tttgggctgg
     5821 aattattttc atcacaagat gaaggtgtaa aattagaatt taacaaatta ggaaacattg
     5881 taactttaga cagaagcaac tttaaaaaag ttttcggtgt tgaatatgga acaaatagaa
     5941 aagaatatat aaatattgat gaaaatacta atatcaaagt actagcggat agaagtattt
     6001 tagaaatttt tataaatgaa ggagaagtag tatttactag tagaatattt gctaaagaaa
     6061 attctaatca aataagggtt tatagtgata aaatagtaga gtatgagtat actaaattta
     6121 aattaaagca aggtattgaa ttataaaaac acattataag gggtagaaaa gatgaaaaaa
     6181 gtaattagca taggagaagc tttaatagac tttataccga atcaaaatgg tggtaaatta
     6241 aaaaatgtat cagaatttag aagagtagca ggaggagctc cagcaaatgt tagtgctgtt
     6301 gtagcaaagc ttggaggaaa atcgagtttt atatctaagt taggaaaaga tgcatttggt
     6361 gattatataa tagatgtatt aaatgaagta aatgtaaata ctgattatgt attgagaaca
     6421 agtaaagcaa atacaggact tgcttttgta tcattaaaag aagatggtaa tcgtgatttc
     6481 tcattttata gaaatccaag tgcagatatg cttttagaag ctgatgaagt taaaaaagag
     6541 tggtttaata attgtcatat cctacatttt tgttcagtag atttaataga tagtccaatg
     6601 aaattagcac ataaaaaagc aatagaatac gcacttgaaa gtaatagtat aataagcttt
     6661 gaccctaata taagattacc tttatgggat agtgagcaat cttgtaaaaa ggcaatatca
     6721 gaatttttac cttttgctca tattgttaag atatctgatg aagaattaga atttataaca
     6781 ggagaaaatg atatagaaaa atcattacat aaactctttg tggggaatgt tgaactagta
     6841 ttatatacaa aaggaaaaga tggagtagat gcatacacta aaaaagtaaa aggtatgtgt
     6901 aaaggggtta aagtaaatgc aatagataca accggagcag gagattctta tataggttca
     6961 tttttataca ccttattgta taaacaaata acattgaatg atataaaaga aatgaaacaa
     7021 gaaacgttaa atgagtattt agaattttct aattattatg cagctcgtag tacaactatt
     7081 aaaggtgcga taagttcata tgcaacaaag gaagaaataa ctgaatttat aaaaaattta
     7141 tagtaggaat gagtaatagg ttttgaagga agtaacatgt aaagagtttt taaggatgtt
     7201 tactatgaaa ataattaaca tataaaaagc aataaagagg ctgtctcaaa atagagataa
     7261 gcctctttat cattgttgaa atgataaaaa atattttaat tattgttact ttttagaaga
     7321 atatatatct tattttaaat aagcaaatta tgtaagattt atttatatta tcaatacttt
     7381 acttggagta ttatgtcttt agcagttatc tacttcaatt gaattatcta aaatataata
     7441 aatttgtttt gcaaattgac gagaatgttc ttctttttct ttattcataa aattaggttt
     7501 acctaaatca cgttcaacaa gcattcctat atattttaac tgtgaatgct tacagtagcg
     7561 cttcatacca ttttcccaaa tatcagcacc cttttcaggt ctatatccac atgttgtaat
     7621 aagtgctact ggttttcctg cccatagagt ggggcctttc tcattaccat agtatttatt
     7681 cattccatac accaatctat caagtagtgc tttcattgga gaagtgcagt accaagaata
     7741 aattggagta gcaaaaacaa ttaaatcaca cataagaatt ttcttgaaaa ttttctgaac
     7801 atcatcttta caataacatc caaaatcaaa taagttatcc tgacattttt tacaagcaat
     7861 acatggctct attttacaat catataacca tattacttca atatttgaat tataattttc
     7921 taattcctca ataaaaggtt ttaaaatagc agcagtatta ccttttttct taggacttcc
     7981 cataagaatg catacttcca tattgatacc tccacagtat tttaaaatag tttttagtgc
     8041 ttaatatagt tttaggatac tatgagaata cgttaacaac aatacacttt gtatatttgt
     8101 aataaaattg aaacaaattg ttttatcaat aaaagttgga atgtatctat aattaaaaat
     8161 attttaggta tgtttttaca tatatgtgtg tagttaacta tggtaattta ttgtaaaatt
     8221 atcttatagg attataattg cttaatttat gattatattt aaaatcttgg aggtaaaaaa
     8281 tatgtactca aatgaagact ataaatttat agaagaaaat gcaccatgtt atatatatga
     8341 tgagactaat ataattgaaa aatgtaagga attgaaaaat aatattttag gttttgattt
     8401 tttatattct ataaaaacaa atccatataa caaaattgta aaaagcattg cagagcaagg
     8461 ttttggagca gatgcttctt caactaatga agttatgatt tcatcagaat gtggagtttc
     8521 taaagatgat atttattatt catcaccagg caaaacagag attgatatag aaactgtttt
     8581 agataaatgc ataattattg cagatagtat tacagaaata gaacttataa ataagatatc
     8641 taaaaagtat aatcagaaag ttaaaatagg aataagaata aatcctgatt ttactatgga
     8701 tgatgatagt gctaaatcaa gcaaatttgg tatagatata gaagatatga gcagtgtttt
     8761 taagatgtta agtatgtgtg attatgtatc tgtagttggg attcatatac atattaaatc
     8821 tcaggtattg gattttgaaa agttaggaca ttattatgaa aaatgttttt ttatagcttt
     8881 aaatttaaat aaaagtaagt atattaatat tgaatttata aattttggta gtggacttgg
     8941 agtagcttat gataatctta gagaaaatcc acttaatctt aaagagcttt caaaattaac
     9001 tcaaaagatt gcttttacta attcaaacac actaaaatca agacttctta ttgaatcagg
     9061 acgtttcata gtttgtaaat caggtaatta ttatgttaaa attgtagata ttaaagtatc
     9121 aagagggaaa aaatatctta ttgtaaaaaa tggacttaat ggatttatga gacctattat
     9181 ttctaattta attgaaaata ttacttgtgg agtagaatta gaaggtcaag agccattata
     9241 tacatcaaat catgcctttt ctctttcagt attaaataat ttaaaagaag aagagtatgt
     9301 aaatgttgtt gggaatctct gtacatcatt agatgtaatt gcaaataatg ttaaattgaa
     9361 aaaagcaaac ataggagatt tgatttcaat tagtaatgct ggaagttact cctattcttt
     9421 atcaccgcta ttattttcaa gttatgaatt tcctaaacaa tattataaaa gaaaagatgg
     9481 atatattgag taaacaatag cataaattaa taaacttagt attattgaaa aaatttaaaa
     9541 tgcagggggt gtaattctat atggaattgt ggaaaagaaa tttatttgta tgttggatag
     9601 gtatgttttt tagttctata gggatgagcc aaatagcacc tatactttca ttatatatta
     9661 aacagttggg ggtaactgat gttagtttaa tacaacagta ttcaggtata gtttttggat
     9721 gtacctttgt agtagcagca ttcttttcgc caatttgagg gaaagctgct gataaatatg
     9781 gaagaaaatc aatgctttta cgtgcaagta ttgggatggg aatagttata tttaccatgg
     9841 cgtttgttca aaatgtctat cagttattag gattaagaat acttcaagga gtttttacgg
     9901 gttatgctac tgcatgtact actcttattg caacacagac agataaaaat cattcaggat
     9961 gggctttggg tactcttgca actgcatcaa ctactggctc attaattggt ccaacagtag
    10021 gaggatatat agaatcaatc ttaggtctta aaagtacatt tattataaca ggaggattgt
    10081 tgtttgtatc atttatcatc tctattttat ttgtagtaga taattttaaa cctaaagaaa
    10141 taaaagaatc aatttctata cctaaagaac aattaaatat attgccaaat aaatttttaa
    10201 ttgcatctat ttttgtaacc acattcatta ctcaactagc tttatactct atagaaccaa
    10261 ttgtaaccat atatatatca caacttacaa attttgcatc aaatgtagct ttaatagctg
    10321 gattgacatt ttctgcatca ggattagcaa atttattagc agctcaaaaa cttggaaaaa
    10381 tgtcggataa aataggtcct caaaaagtat tgttaatttc acttttatgg gctgggatta
    10441 tatttatacc acaagctttt gtaaaaactg catggcaact gatgttgttg agatttttat
    10501 tagggttgtc agttgcaggg cttaatcctt ctgttaacag cttgcttaaa aaaattgcac
    10561 cagaagaata tgtgggaaaa atatttggat ataatgcatc tgcccagtat ataggctgtt
    10621 catcaggagc atttttaggg ggacaaatat cagcacattt aggtattaga acagtatttt
    10681 tttcaacaag tttattgttg tttatgaatg cactttggat gtataaattt actggattgt
    10741 ttattaaaga taagactaaa taatcatact atttacattt ctaattttat gatgtaaaat
    10801 aaatattttt tatgtaattt atcatagaat tatataaaaa ataaccctat ggtaaaaatt
    10861 ggaaaatatt attatcgaca aaatctttga cttaaaaact atattgcgat agtattaatt
    10921 taataaaagt ttgagagggg cgtaaggtta tgacaatttc aaaaatacca caagcagaat
    10981 taaaagttat gaagtttatt tgggaaaaaa atgatacagt aacatcaaaa gaaactattg
    11041 aagctatgga acgaaaatat ggttggaaac aaacaactac attgacactt ttatcaagac
    11101 ttgttaaaaa acatttttta agtgcagaaa aaatagatag atacactcat tatacaatag
    11161 ttgttggaca taaggagtat ttaagtgttg agacaaaaga ttttttaagc aatatacacg
    11221 acaattcttt acagagttta atttcagcac tacatgatga tgaagttgtt gataaagata
    11281 tgttggattt atttgaaagt cattttaaaa atttagaaga agagtaatta tatcaggata
    11341 gaactctaag aatttaatta atctatggct gaaaaccata aatggtatta tttaatcagc
    11401 tactcactat tcataaagta atattaaata ggttctctaa ttagtaaaat gcctaaaata
    11461 attggaatag ggagtatcaa gttaaatgat accacttatt taaataagaa atgattttta
    11521 aaatttaaaa taattttata acttgtcaat attcattaat ctaaagtatt atttctattt
    11581 aaccatatca aaatatattt tactattttt aattattaca atataaacct gtcttttgtt
    11641 attataggta aactaaaagt aagaaatgat ttttatattt ttaaacttta ttattggtaa
    11701 cttatctgta aatattttaa aatgtactat agaaagttgt taaaaatatt aaataattat
//
LOCUS       DAELTA010000113        11730 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.113, whole genome shotgun sequence.
ACCESSION   DAELTA010000113 DAELTA010000000
VERSION     DAELTA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.113"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            147..842
                     /gene="cas6"
                     /locus_tag="KPG13_002630"
     CDS             147..842
                     /gene="cas6"
                     /locus_tag="KPG13_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0986332.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            833..2593
                     /locus_tag="KPG13_002631"
     CDS             833..2593
                     /locus_tag="KPG13_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HBG0986333.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLN
                     KIRLWIKTNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIID
                     GKNYGLPNDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEE
                     VIYISSEKGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIP
                     IEYERHNLKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDIL
                     IRHREGLYTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFH
                     GGSNMADKIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSL
                     INPILNAQNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLL
                     IGGYLYYNLIYTKSEENENE"
     gene            2580..3575
                     /locus_tag="KPG13_002632"
     CDS             2580..3575
                     /locus_tag="KPG13_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HBG0986334.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPK
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3577..4317
                     /gene="cas5b"
                     /locus_tag="KPG13_002633"
     CDS             3577..4317
                     /gene="cas5b"
                     /locus_tag="KPG13_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG0986335.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4341..6899
                     /locus_tag="KPG13_002634"
     CDS             4341..6899
                     /locus_tag="KPG13_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0986336.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   7089..10090
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7089..7118
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            10175..10669
                     /locus_tag="KPG13_002635"
     CDS             10175..10669
                     /locus_tag="KPG13_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG0986337.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            10704..11702
                     /gene="cas1b"
                     /locus_tag="KPG13_002636"
     CDS             10704..11702
                     /gene="cas1b"
                     /locus_tag="KPG13_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG0986338.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
BASE COUNT         5048 a         1050 c         1793 g         3839 t
ORIGIN      
        1 tttgtcgaat aaaaaataaa aaattttaaa ggaaaaagtt aaaaaataaa gaattttata
       61 actataaatt atattttaga gtttattgtt attaaataga tgaaaaaatc ctaatatgga
      121 ttatcagaaa atgaggaggt tgttaattga taaaatgtct tgaaatgtct gtaaaagtgt
      181 atcttcttga aaatatttct ttagataata catcaaaaga aatacgtaaa ttaatagatg
      241 atacattaat tatagaagaa aaatataagg aatttcataa taaagctgga tttaagaatt
      301 attcttttaa tttattatac ccattagaaa aagataaggt ttataagaag gattgtatat
      361 atacttttat tattagaact gtagatagtg aattaggaat gtattttatg aataatttaa
      421 aaaatcagca ttctgataaa ataaaggtta ttacaatgag taaaaaagtg atccctaaaa
      481 aacatataga aagtatttac tctgtcactc caataatagc taaatttgag gatgggtatt
      541 ggagaagtaa taaaacagta gaagatttag aaaaaagaat cagagaaaat ttaataaaaa
      601 aatataatga atttactaac agtaaaatag atgaagattt tgaattattt caagttatta
      661 aatttataaa taggaaacct atttctacta aatataagaa tatatcttta cttggtgata
      721 aaataagttt taaagtatca gaaaatgata tggctcagga aattgcaaat tttgcacttg
      781 gagttggagt cggagaaatt aattctcgtg gatttggttt tgtaaactat agatggttat
      841 aggaggtgaa aacttgttaa aggattgttt agagattttt gaaaaagaat acaaatcttt
      901 aggaaataga ttgataactg atagctatgt cctatcctat ggaacatata tattggtgaa
      961 aaatttttcg aatatagata tattagaagt ttctaaaaat aatgtggata gtacagaaga
     1021 tatataccat aaattttgtg aactggatta tttatcaaaa ctggtgtcta tagataaatc
     1081 agttgattcc aaaaaaataa tacatagtaa taattatctt tcattttttg taaaaaagga
     1141 aaacttaaga cctgataaaa ataataatgt aaaacttaca gaagaaatta ttgataatta
     1201 ttatgatgct ttgttaaacc caagaattaa atataaatct gataaaagaa aaaaagaaat
     1261 ctatgattca atagaaaata aatatggtaa agtggatgaa aataaattaa ataaaataag
     1321 actatggata aaaacaaata tatttgattt agttgaatct gttaaaaaag ataaaaatta
     1381 tttaaaaata ttttttgatg aaaaaataga gttatacaat gatgaaaacc aaaaatattt
     1441 atatccaaat atatataata aaaatgattt caatgtaatt attgatggta aaaactatgg
     1501 gttaccaaac gataatatgc aattaaatga aaaaaagcca tttttgaaat taaaaacaag
     1561 aattagaaat tcaactcctt atttgattag tgatgatgaa gttttgttac agaaagcttt
     1621 ttttgattat ctaacgaata aagcagcgaa tggagaagaa gtaatatata taagtagcga
     1681 aaagggaatt catagaaaaa ctgatatacc aaatgaattt agtggttata tactgagaat
     1741 aaaaaagggt aaagaattag aaatacatga ttttgatact gtggtaaatt taaacactaa
     1801 aataaaaaga ataaatgtag aatgtataat accaatagaa tatgaaagac ataatttaaa
     1861 accaaattta gagataggat atattaataa tcttataaaa ttggaaaaaa cgattgatac
     1921 tgtattcttt aataaattta tgtgcaatag ttattttaaa gaaattaaaa atataaactt
     1981 aagtgattta gttctaaggg atatacttat tagacataga gaaggtttat atacctggtt
     2041 ttataaaggg agagatagta tagtaaagca aactttttca aactattcta tgggtataat
     2101 tgaaaattct ataaaaaaca ataactacat aagagcaaag gagcaatata atttaagaat
     2161 agctataata aattattttc atggaggaag taatatggca gataaaataa aagttattag
     2221 tgaatcttta tttgaaaaaa taaattctat aaaaacgaaa tgtatacaaa gtgatgaaga
     2281 atattatttt gcagttgggc aattagcatc gtattttata tctaaaaata agtcaaaaaa
     2341 taaaaatcat tctctgatta atcctatact aaatgcacaa aatagaaaaa aaatcatgtt
     2401 agagatagaa aaattattta aaaaatataa ttatgatata agttatgaca acaataggcg
     2461 atttaagaat ctatattcta tgattgtagg atatgaggta gaagaaaaaa aagttaatag
     2521 agatttatta attggtggat atctatatta caatttaata tacacaaaaa gtgaggagaa
     2581 tgaaaatgaa tagaagagta tatggagtat taggtataaa atctataatg tcaaactgga
     2641 atgctgattt ttctggatat ccaaagacga catcaacagg agaagttttt gggagtgata
     2701 aagcacttaa gtttcccatg aaaaaaatgt gggaaaatga aggtgaaaaa gttttatatg
     2761 ttaaatcatt aaaaatagat gaatctaaga aaggagaaat aaaaattaga ccaaaatcat
     2821 taaaagagag atatgaagaa ttgtttgata tagaggacct aaaactagaa aagaatcaac
     2881 aaaaagtact ggaaaatcta tttaaagcta ttgatgttaa aaattttggt gcaacatttg
     2941 cagaagaagg aaataatata ggaattatgg gcgcagtaca aattggacaa ggatttaata
     3001 agtatgaaga tattaatgtt gaagaacaac aaatattatc cccatttagg gatccaaaag
     3061 caaaagataa gaaaaagtca acaaatgaag aagaaaaaga agatgcaaat tcaactacat
     3121 taggaaaaaa aatagtaagt gatgaggctc attactttta tccattttca ataaatccaa
     3181 aagcttatga agtttttgtt aatatgggtg tgactgatgg atatactaaa aatgattata
     3241 aaaaatttaa agaagctgct ttagtaagtg caactgcatt caatacaaat tcaaagcttg
     3301 gttgcgaaaa tgaatttgca ttatttgttg aaactgatga agagttatac ctaccagatt
     3361 tggcacaata tataactttt gaaaagagag aaaaatcaaa agatttaatt tgtataaata
     3421 taattcatct tatagcagaa ttaaaagatg atataaagag cgttgagata tattataacc
     3481 catataaaat agaaataaag ccttttaaag atgaaatatt acctaagata gataaagtta
     3541 aatttatgaa catatttaca agaaaagagg tataatatgg aggttttgaa atttaactta
     3601 agtggaaaaa ctgctttttt taaaaaacca gatgtaaata gcataattta ttttacgtat
     3661 ggaaacatac ataaaattgc acttatgggt attttaggag cttgtttagg acttggtgga
     3721 tataatcaac agagtaagga agatgagtat ccagaattct actctttact taaggatata
     3781 aaaatgtcaa tagttccaaa gcatgaattt ggatatataa ataaaaaagt ccatatattt
     3841 aataattctg taggatatgc atcaaaagaa gaaggtggaa atttaataat aaaagaacag
     3901 tggcttgaaa atgttagttg ggatatatat atacaattag aagattcaac tacaagtaga
     3961 gagcttaaaa gaagattttt atctaataaa tttgtatata taccttactt aggcaaaaac
     4021 gatcatatag ctaatataag tgaaatagag ataattactg atttttacaa aacagaagat
     4081 attaacagag tggacagttt ctttataaga gaggattttg agtttgaaaa atcttatttt
     4141 gatgataatg atgattataa agagccattt aaatatcagg aaaaacttcc ttatagactg
     4201 caccgaatga ctaatcaata tattttggaa actatcatat ttactaatat gtcagttaaa
     4261 ttgattaata aaaaagaggt atatcaagta aagggtttaa atttaatctt tatttaaatt
     4321 tataggttta ggagaattat atgtacttta aaaaagttga gttgtttgat ataagtaata
     4381 atataaataa ttgcaaaaat atttatgctc atataaataa agacaggaag ccagaaactt
     4441 taaaagaaca ttcagactta tcatactcat atcttatgaa attatgtgta aaaaaagatt
     4501 taagctcttg cctagagaga atagagaata aattttttaa aaaagattct tttttaagta
     4561 agaattttta tagagagatt atcttgaata tggtttatat gcatgatata ggtaagatta
     4621 attcttcatt tcagtttgta aaaatgcaaa ataaaaattt taaaaaaatt tcacttagat
     4681 atactaaaca ttcttttatc tcttcaatgc tatattttga ttactatttt gaaaagctta
     4741 aaaaatatga ttttgaagaa aaaaaaatat tgagtttatt tttgatgata aatacatata
     4801 caatatcaaa acatcatggg gttctgaagt catttaaaga atttttattt ttatttaaag
     4861 atgagtttat aaacattaaa gaaaatttta aagatttatt taaaaacaat tataatttag
     4921 agcttaaatc agttcccaaa actatagaaa aaatggtaag aggtgtagaa gcgtatttag
     4981 atgatagcca tataaaggat aacttagcta tagacttata tatatattca aaattgatgt
     5041 tttcaattgt tacatcttgt gatttttatg ctacatcaga atataatagt ggtaattcta
     5101 tagatgattt tgggacaatt gataattata ataagtatta tgatgtatta aaaaaatctg
     5161 aaatatataa ggggatgaaa aaatataggg aatacttaaa taacaatagt gaatgcccat
     5221 ataaagagaa tgatataaac agattgagaa cagaaatgag tatagaagct gaagatgcaa
     5281 ttacacataa tacgagaaaa agaatatttt atttagaagc accaactgga agtggaaaaa
     5341 ctgttacatc tataaatcta gcactgaaat ctatagagtt aaatcaatct ttaaaaaaga
     5401 tattttatat atttccattt aacacattag tagagcaaac aaaagatgta tttataaatg
     5461 aaatatttaa ttctgttaaa gacatacaaa aagatgttgt agttataaat tcaataacac
     5521 caatacaaac agaagataaa gaagaagata ataaaaatgt tgaatattgt gttactaaac
     5581 aagaaattga ttatgataaa tcattgttaa atagacaatt tttacactat cctatagtac
     5641 ttacaactaa tataggtttt tttaactatt tgtttggtgt atcaagagaa gaatgttttc
     5701 cattgataca tatgataaat agtgtaatta tattagatga aatacaaaat tataaaaatg
     5761 agatatggaa agaaattatt ttgtttttag aaaaatatgc agatatatta aatataaaat
     5821 ttattataat gtctgcaact ttacctagat taaataaatt aggatgtaat gatgataatt
     5881 ttgcatatct agtaaaaaac agagaaaaat tttttaaaaa tcaccttttt aaagatagag
     5941 taaacataag ttttgagatg ttaaattcag aaattaatga tattgaagaa atatcagaaa
     6001 aggttataga agaagcagaa atatataata agatactgat agagtttata aagaaatcat
     6061 cagcactaaa attttataaa aatattaaag aaaaattgaa gtataagtta gaagatgttt
     6121 ttttattaac aggtgatgat aataaattag aaagaaaaaa aataataaat aaaattaaag
     6181 atatgaactc tgtaattttg gttgctacac aagttgtaga ggctggagta gatatagata
     6241 tggatttggg atttaaagat atatctacaa ttgattctga tgaacaattt ttgggaagaa
     6301 taaatagatc ttgtaaaaaa ttaaattcaa aagtatactt ttttaattta gatgatgcaa
     6361 gtaagatata taagaaagat gttagaaaag agagacattt aactcttaat gaggaaagta
     6421 atagaaaaat tattttagat aaagactttg aaaagtatta tgataaaata atagccagaa
     6481 tagaagaaaa taagcataaa cataatgaca agaatataga ggtttttata aaagatattg
     6541 tgggaaattt gaattttaca gaggtaaaaa aatggatggc tttaatacca gatttaaaag
     6601 agtatacaat ctttttaaat actatagtta aagatgaatc tggaaatatg attgtaggaa
     6661 gtgatgtatg gtatgaatat atatcactac taaaaaatga taatgttgag tattctgaaa
     6721 agagggtgaa aatgtctgaa attatggaaa aactagatta ttttacgtat aaagttcaga
     6781 aatttgataa ttcatttaat gatttagttg gagatatttt ttatattgat gatggaagta
     6841 agtattttac agagggaaag tttgatagaa gtaaatttaa tcaaaatgaa tttctataat
     6901 gtatttagat tatatagaga atatttatcc caagttaatt aattagaatt agtttaaaac
     6961 tattgaaata tagatatttt atagcttata tattttttct aggattagct gggataaaaa
     7021 atctgtatat tagtgttttc agtgtattta aggggattat atttttaaga agtactgatt
     7081 ttaaagtggt taaacagtaa catgagatgt atttaaattt ttactacata attaaatcgt
     7141 agtcaatacc gtacaggtta aacagtaaca tgagatgtat ttaaatctct gaatagccaa
     7201 tcaactttgc tgcattactc ttgttaaaca gtaacatgag atgtatttaa atgataatag
     7261 ttatatatat tttttacaat atattattgt taaacagtaa catgagatgt atttaaattt
     7321 acttctcctg ttttgaaatc tatatattca tcatgttaaa cagtaacatg agatgtattt
     7381 aaatataata ttgatacaaa tattttgggt aaatggactt gttaaacagt aacatgagat
     7441 gtatttaaat tcgatatggt aacaaattta taaaagatat agaatgggtt aaacagtaac
     7501 atgagatgta tttaaataaa gaaaaacgaa attggtgatt ttgaagcaat gtgttaaaca
     7561 gtaacatgag atgtatttaa attagtaact acaatgcttt tccttttgaa gatgatatgt
     7621 taaacagtaa catgagatgt atttaaatta ataatgcacc tattttaaca gtacctggtc
     7681 taaagttaaa cagtaacatg agatgtattt aaattcaagt ctaggagaca acataaatag
     7741 tacagatggg ttaaacagta acatgagatg tatttaaatt agtaaaacca gatggaactg
     7801 ttgtaatacc accaaagtta aacagtaaca tgagatgtat ttaaatttaa aaacatcttg
     7861 aggaatatct ttaacagtaa ttgttaaaca gtaacatgag atgtatttaa atgcattttc
     7921 aggtgcatga taaataggta aattagatgt taaacagtaa catgagatgt atttaaattt
     7981 tttgtaatta gccagattat caactcccaa aatagttaaa cagtaacatg agatgtattt
     8041 aaattaaata tcgtccttcg tagtcaaagc aacaccagaa ggttaaacag taacatgaga
     8101 tgtatttaaa tcgtaaacaa aatttatcaa gagaagaatt tctaaggtta aacagtaaca
     8161 tgagatgtat ttaaatttat atttgataag agtatcaaat cgacttaaaa tagttaaaca
     8221 gtaacatgag atgtatttaa attcaatctt aatacacaga catttttaaa aacaaaacgt
     8281 taaacagtaa catgagatgt atttaaataa atagacaaat attgaatagc tctgtcttca
     8341 tcaagttaaa cagtaacatg agatgtattt aaatatattt acaaaatctc taacatattt
     8401 tttaacttct gttaaacagt aacatgagat gtatttaaat tcttttaaag agtcctcggg
     8461 agcctcgaaa acttcagtta aacagtaaca tgagatgtat ttaaattact caaataaatt
     8521 tctaatttgt ttatctacta acgttaaaca gtaacatgag atgtatttaa attagaattt
     8581 acaacaatat aagcctcttt aaccataagt taaacagtaa catgagatgt atttaaatat
     8641 gtttttagta gtacatttaa taaaactttt taaagttaaa cagtaacatg agatgtattt
     8701 aaatgaagga accgtacctt gataaatcac gaccgtaccc gttaaacagt aacatgagat
     8761 gtatttaaat tgtatatttg atgttgttat ttctcctgtg tataatgtta aacagtaaca
     8821 tgagatgtat ttaaatgaag ttccaccccc accaccccca ctactttgag aagttaaaca
     8881 gtaacatgag atgtatttaa attaacaata gagtaaatat aataaacaag catacaaagt
     8941 taaacagtaa catgagatgt atttaaatcc ccttttgcat ggtactagac acgcatatat
     9001 ggtggttaaa cagtaacatg agatgtattt aaatacgcct atatgcggag taacagattt
     9061 ttgcctggat ggttaaacag taacatgaga tgtatttaaa tactgttttg ggatagactg
     9121 gaattaaatg gtctgtgtta aacagtaaca tgagatgtat ttaaattttt ggaatgaagc
     9181 atcaacagat aataaagaaa aagttaaaca gtaacatgag atgtatttaa atcaaggaaa
     9241 caaaagagaa gtgaaaagaa atataatgtt aaacagtaac atgagatgta tttaaataat
     9301 cttttaaatg gaaattaagt aataaaagag agggttaaac agtaacatga gatgtattta
     9361 aattcaatta aataagcatt aatcttagta aaatcaaacg ttaaacagta acatgagatg
     9421 tatttaaatt ttcttgtgtt aattgaaccc ccattttaag ctccagttaa acagtaacat
     9481 gagatgtatt taaatcagaa tacagcttct atatctggat acatttgtct tggttaaaca
     9541 gtaacatgag atgtatttaa atgtgaaaag taatacaggt atagagtcta aacgctcggt
     9601 taaacagtaa catgagatgt atttaaatca atgtatcctc ttttttagtt ttggagcctc
     9661 ttttgttaaa cagtaacatg agatgtattt aaattagtta gcacatttta tttcaagcct
     9721 taaaacattc gttaaacagt aacatgagat gtatttaaat taatttgcac acttaacctc
     9781 taatcttaaa gtattagtta aacagtaaca tgagatgtat ttaaataaca caatattatc
     9841 tttaatagaa ttaaacaaat gagttaaaca gtaacatgag atgtatttaa atttttgttg
     9901 gtcttgtgct tcttttaaaa gctgattagt taaacagtaa catgagatgt atttaaattt
     9961 aaaaagttct gttattaaaa catccaaacc aatcgttaaa cagtaacatg agatgtattt
    10021 aaattctgta ccatctagcc aaaccgtttt tgaagttaaa gttaaacagt aacataagat
    10081 gtatctaaat tactctatca taaaatttta atatattaaa caaactaata tgtgatatac
    10141 ttatgtataa ataacaaaat aaataggtga tgttatgaaa attacaggaa ctttgataaa
    10201 ttactatttc cattgtaaaa gacaatgctg gttgcttggt aatagaataa atttggaaga
    10261 aaatagtgaa gatgtaaaaa taggtagact tcttcatgaa ctaaaagaag aaaaaacaaa
    10321 atataaagaa attgcaattg aaaatataaa aatagacaaa ttaacaagag aatatttagt
    10381 agaggttaaa aagtctgatg ctgatataga agctgttaag tggcaagttc ttttatattt
    10441 aaaaaagtta aaagaaaagg gaattgtaag aaaagggaaa attgaattta tagaaaaaaa
    10501 taaaactaat aaaaaaatag tctttgtaga tttgagtgaa gtatctgaag aacagcttat
    10561 atctatagaa agagaaattg ttaatttaat aaatagtgat aaagcaccat gtcctgattt
    10621 aaaaaaagga tgtaaaaaat gtgcctatta tgaatattgt tatatataaa gaggtataag
    10681 aaacatataa ggaggtgtta atattgggaa agacaaaata tattacctca atgggtgaac
    10741 tcacaagaaa agataattct atatgtttta gaaaaaatgg aaggaatgta tatataccag
    10801 tagaaaatac tagtgaaata ttctgtctaa atgaggtatc tttaaattca aaattactgg
    10861 attttttatc aagagcaaat attgttgttc atttttttaa ttactatggt ggatatagtg
    10921 gaacttttta tcctaaagaa tatttaataa gtggaaaagt aacagtggca caggtaaaaa
    10981 cctttgaaga tagaagagaa gaaatagcaa aatccatagt caatggtata aggattaatg
    11041 caataaatac attatcgcat tactataagc atggaaattc agaaatacaa ccattattat
    11101 cacatctaaa aaaagattct ataaacaagt tagaaaaatc aaataatata aaagaaatat
    11161 tatcagtcga aggagatatt tggcaaacat tttatacttc ctttaagtat attttaagag
    11221 aggaatttat ttttaataaa agagttaaga gaccaccaga taatccctta aacgcaatga
    11281 tatcatttgg taacagtatt ttatatgcta aaactgtaac tgctatatat aatacacatc
    11341 taaatcaaag tgtaagtttt ttacatgaac caagtgaaag tagattttct ttaagtctag
    11401 atttaagtga aaattttaaa catgttataa cttttaaaac tatatttgat ttagttaata
    11461 atagaaaaat aaatattgaa aaacattttg ataaaaattt gaactactgt gttctaaatg
    11521 atgaaggaaa gcaaattttt ataaattctt tagaagaaag attagaatca gtcaaagaac
    11581 atccaaaatt aaaacgaaaa gtaagtttaa aaacctgtat aaaattagat gcttacaaaa
    11641 tgttaaagtg tataatggaa gaaaaaagtt ttattccata taacataaaa gacggattat
    11701 aatttaaaca taaagtaagt attttaatat
//
LOCUS       DAELTA010000114        11696 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.114, whole genome shotgun sequence.
ACCESSION   DAELTA010000114 DAELTA010000000
VERSION     DAELTA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.114"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            260..1099
                     /locus_tag="KPG13_002637"
     CDS             260..1099
                     /locus_tag="KPG13_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG0986339.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1096..2277
                     /locus_tag="KPG13_002638"
     CDS             1096..2277
                     /locus_tag="KPG13_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986340.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2581..3483
                     /gene="ptb"
                     /locus_tag="KPG13_002639"
     CDS             2581..3483
                     /gene="ptb"
                     /locus_tag="KPG13_002639"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG0986341.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3505..4584
                     /gene="buk"
                     /locus_tag="KPG13_002640"
     CDS             3505..4584
                     /gene="buk"
                     /locus_tag="KPG13_002640"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG0986342.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4574..5251
                     /locus_tag="KPG13_002641"
     CDS             4574..5251
                     /locus_tag="KPG13_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0986343.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5294..5509
                     /locus_tag="KPG13_002642"
     CDS             5294..5509
                     /locus_tag="KPG13_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0986344.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5538..6617
                     /locus_tag="KPG13_002643"
     CDS             5538..6617
                     /locus_tag="KPG13_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0986345.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6617..7369
                     /locus_tag="KPG13_002644"
     CDS             6617..7369
                     /locus_tag="KPG13_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0986346.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7370..7927
                     /locus_tag="KPG13_002645"
     CDS             7370..7927
                     /locus_tag="KPG13_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0986347.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8097..9443
                     /locus_tag="KPG13_002646"
     CDS             8097..9443
                     /locus_tag="KPG13_002646"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG0986348.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9710..11542
                     /gene="glmS"
                     /locus_tag="KPG13_002647"
     CDS             9710..11542
                     /gene="glmS"
                     /locus_tag="KPG13_002647"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG0986349.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
BASE COUNT         4608 a         1347 c         2323 g         3418 t
ORIGIN      
        1 taaaactgaa ttaaggcgcg agtactattt tgtattcgcg ccttgatggt ttgagttatt
       61 tgagtaaaaa ctaactttaa taagaaatat gtatttatta agttgatatt tttaatgtgt
      121 ttagagatta tttaacaatc tccaaatata aaactagaat tatttacata aaagttaaat
      181 agtttgtata gagttagatt tatatagtat aatacttagt gtaaataatt aagaagttat
      241 caaacaggag gataaaactg tgttagatat aaattctttt ttaaatggat tttttaatat
      301 aatattaaga atgagtatat atgatttaat tgacatatct atagtagcgt atatttttta
      361 caaaatattt atgtttataa aagatacaag agcagaacaa gtgtttaaag gtataatatt
      421 tcttttatta gcaactcaac ttagtaacac ctttaaattg cataccgttt attggatatc
      481 tcttaaagct ttagattatg gagttatagc tgctcttata atttttcaac cagaatttag
      541 agcaggactt gaacatatag gaagagcaaa gtttaattta tttggaaaga atgtaaatac
      601 ttctgaagaa actctcaata gaaatataga agaaattgta gaagctttat attcactttc
      661 tagacaaaaa ataggagctc ttatcataat ggagagagaa actagaataa gtgatataat
      721 aaatacagga actattattg atgctgaaat ttctaggcaa ctattaataa atatatttat
      781 accaaataca ccattacacg atggagctgt tgtaattaga gattctaagg taaaagcagc
      841 tgcatgtttt ttgccattga cagaaagtaa ggatttaagt aaagatttgg gtactagaca
      901 tagagcagga ataggggtaa gtgaggtgtc tgactgtata actttgattg tatcagagga
      961 aacaggtggc gtatcaattg ctaaagcagg taagttatat agagatattt ctagagaaag
     1021 aatgatgaat atattacgta gtaatttaaa aacaaataca gaaacaagaa gttttttcaa
     1081 aggtggtata tttaaatgat aaatagacta aaaaataata ccaaaataaa aataatatca
     1141 tttttaagtg caattgcatt atggatgtat gttatggctg tagttgaccc tgaagaaact
     1201 aaagggtttg aagatatacc tgtatctata agtaatatga gtgatttaaa ggataaaaat
     1261 cttgttatat atccagacga aaaattaaca gcagatgtat atataaaagg tagtttatca
     1321 aaggtacaaa gtgttaaaaa agaagatata catgtatatg gaaccattga agacccaata
     1381 caaggacaga aggaagtata tttaaaagca aatattcctc aaggtgttac tctagaattt
     1441 aagcatgatg ttttagtagt aaatcttgaa aaaaacataa aagaaaaaag agatataaag
     1501 gtagaagtaa gtggaaattc aaaaagaaat ataaataaaa ttgaacttag taaggaattt
     1561 gtcgatgtat caggtccaag aactctagtt aaagaagtca aaagtgtaag agctaatcta
     1621 gatgttggta atgagacaga tgatttttct aaaaaattgc aattaatacc attaaatagt
     1681 gatggaacta aggttgaagg agtaacatta agttcatcct ttgtgactgc aaagatagaa
     1741 ctattgaagg aaaaaattgt cccaataaga gtaagtctaa atgataataa agataatgat
     1801 gtactgaaaa actataaatt aagtaaggaa caagttacaa taaaaggaaa gaaagaagat
     1861 atagacaaaa taagttatat taagacaaaa cctattgact tagctgattt ggctaatggt
     1921 gaagaaaaag aagttagctt agaaattcca gagggtataa ctcttgagga tagctctata
     1981 acaattaaat taaatgatac aaaacaattg agtgcagaat ttttatataa tgcgggagat
     2041 attgaattaa gaaatatccc aacaaatatg gataatccaa atgtaatttc tgatgtagat
     2101 ataaaagttg ttgtagagtc aatggaggat ttgagtaagt taaataaaaa agatatttca
     2161 ttatatattg atttatcaca aggtcctgag gaagatagta aatataaaat aaaatatgaa
     2221 acaacatatc aatttaaaaa aataaatata gaacctaata ttgttgaagt ggaatgaaca
     2281 aatatttcga atatttagtt gttacaagat gcgacaaaaa aacctataaa attaagtttg
     2341 ttaattttat gtttttgttg catcttgttt attttagtta tattataaaa taaggacaat
     2401 gagatatttg ttaaatctaa aaagcgctag atatatgaaa aaagactgat aaatagattt
     2461 aaataaatat tttgaattaa atatttcaat tatctctagg aaaacgttgc tgaacgagtt
     2521 ataatttaat tgagatataa aaagttctat ttcaaaaaat tcaagatgag gtggaaatgc
     2581 atgagaagtt ttgaagaagt aattaagttt gcaaaagaaa gaggacctaa aactatatca
     2641 gtagcatgtt gccaagataa agaagtttta atggcagttg aaatggctag aaaagaaaaa
     2701 atagcaaatg ccattttagt aggagatata gaaaagacta aagaaattgc aaaaagcata
     2761 gacatggata tcgaaaatta tgaactgata gatataaaag atttagcaga agcatctcta
     2821 aaatctgttg aattagtttc acaaggaaaa gccgacatgg taatgaaagg cttagtagac
     2881 acatcaataa tactaaaagc agttttaaat aaagaagtag gtcttagaac tggaaatgta
     2941 ttaagtcacg tagcagtatt tgatgtagag ggatatgata gattattttt cgtaactgac
     3001 gcagctatga acttagctcc tgatacaaat actaaaaagc aaatcataga aaatgcttgc
     3061 acagtagcac attcattaga tataagtgaa ccaaaagttg ctgcaatatg cgcaaaagaa
     3121 aaagtaaatc caaaaatgaa agatacagtt gaagctaaag aactagaaga aatgtatgaa
     3181 agaggagaaa tcaaaggttg tatggttggt ggaccttttg caattgataa tgcagtatct
     3241 ttagaagcag ctaaacataa aggtataaat catcctgtag caggaagagc tgatatatta
     3301 ttagccccag atattgaagg tggtaacata ttatataaag ctttggtatt cttctcaaaa
     3361 tcaaaaaatg caggagttat agttggggct aaagcaccaa taatattaac ttctagagca
     3421 gacagtgaag aaactaaact aaactcaata gctttaggtg ttttaatggc agcaaaggca
     3481 taattttaag ggggaaatag gcaaatgagc aaaatattta aaatcttaac aataaatcct
     3541 ggttcgacat caactaaaat agctgtattt gataatgagg atttagtatt tgaaaaaact
     3601 ttaagacatt cttcagaaga aataggaaaa tatgagaagg tgtctgacca atttgaattt
     3661 cgtaaacaag taatagaaga agctctaaaa gaaggtggag taaaaacatc tgaattagat
     3721 gctgtagtag gtagaggagg acttcttaaa cctataaaag gtggtactta ttcagtaagt
     3781 gctgctatga ttgaagattt aaaagtggga gttttaggag aacacgcttc aaacctaggt
     3841 ggaataatag caaaacaaat aggtgaagaa gtaaatgttc cttcatacat agtagaccct
     3901 gttgttgtag atgaattaga agatgttgct agaatttctg gtatgcctga aataagtaga
     3961 gcaagtgtag tacatgcttt aaatcaaaag gcaatagcaa gaagatatgc tagagaaata
     4021 aacaagaaat atgaagatat aaatcttata gttgcacata tgggtggagg agtttctgtt
     4081 ggagctcata aaaatggtaa aatagtagat gttgcaaacg cattagatgg agaaggacct
     4141 ttctctccag aaagaagtgg tggactacca gtaggtgcat tagtaaaaat gtgctttagt
     4201 ggaaaatata ctcaagatga aattaaaaag aaaataaaag gtaatggcgg attagttgca
     4261 tacttaaaca ctaatgatgc tagagaagtt gaagaaagaa ttgaagctgg tgatgaaaaa
     4321 gctaaattag catatgaagc tatggcatat caaatctcta aagaaatagg agctagtgct
     4381 gcagttctta agggagatgt aaaagcaata ttattaactg gtggaatcgc atattcaaaa
     4441 atgtttacag aaatgattgc agatagagtt aaatttatag cagatgtaaa agtttatcca
     4501 ggtgaagatg aaatgattgc attagctcaa ggtggactta gagttttaac tggtgaagaa
     4561 gaggctcaag tttatgataa ctaatgataa aagaataaga atcataactg gtcactatgg
     4621 aagtggtaaa agtgaatttg cgatgaacta tgttgttaag ttaagagata tggtttctgg
     4681 caaagttgct atagccgatt tagatgtggt aaatgtatac tttagaacta gagaaaagaa
     4741 agagttaatg aaatccttag gtatacaacc aatagatagc tcaattaatg ctccaacatt
     4801 agatttacca gcagtatcag cagaggtaat gtctcctatg gtagaccatt catacaattc
     4861 tgttatagat ttaggtggag ataatgtagg tgctagggtt ataggaagat ttagtcattt
     4921 gttaaaagaa ggcgattatg atatgctttt tgtaataaat gcaaataggg agaaaactca
     4981 aacttctgaa gaagttatcc aatatataaa agaaatagaa aaatcttcta aattaaaggt
     5041 aacaggtcta attaataata ctcatcttat taggtttact actattgatg atgttttaag
     5101 aggtcagaaa gttgcaaaag aagtttctga aaaatgtaat ataccgataa gatatgttgc
     5161 atgtttagaa aatcttgttg aacagttacc aaaagattta gaaggagaaa tttttcctat
     5221 aaaattatat atgagagaag actggatgta gtttgagctc aaatatatag aaagtttaag
     5281 gaggcttagg taaatggcta aaggaaaagt atcttttaat caagaacgtt gtaaaggttg
     5341 tggattgtgt gttgaagcat gtccagtaaa aatcatacaa ttagactcaa atgtaataaa
     5401 caaaaaaggg tataacccag caactgtttt tgaaatggaa aaatgcatag gatgtgcaag
     5461 ttgtgcaact atgtgtccag atgtagttat aacagtagaa agagattaat ttaattaata
     5521 tttaaggggg aattttaatg gctaagatac ttatgaaagg taacgaagca tttggaaaag
     5581 cagctataga agctggttgc aaatatttct tcggttaccc aataactcca caaagtgaat
     5641 taccagaata tttatcaaga gagttaccta aaataggtgg agcgttcgtt caagctgaat
     5701 cagaagtttc tgcgataaac atggtttatg gtggagcagg tgcaggagca agagttatga
     5761 cttcttcatc ttcaccagga gttgctttaa agcaagaagg tataacatat gcagtaggag
     5821 cagaagttcc ttgtgttgta cttaatgtaa tgagaggtgg tccaggactt ggaagtatac
     5881 aaccttcaca agctgactac tttatgtcta caagaggtgg aggaaatgga gactacagaa
     5941 ctccagtatt cgcaccagct acagttcaag aagctgttga tatgataatg gaagcttttg
     6001 acgttgcaga ttattacaga tcaccagtta tggtagttgc agatggtatg ataggtcaaa
     6061 tgatggagcc agttgaattt agagctcctg agaagaaaag agaattgcct cctaaagatt
     6121 gggcaactgt tggaactaaa ggaaaaagaa aacctaatgt aataaactct ctatacttag
     6181 agccagaagt actagaagac cattgttggc atttacaaga aaaatttgat gcaatggaaa
     6241 agaatgaagt acaatacgaa atgtacaaaa cagaagatgc tgaatttgta tttgcagctt
     6301 atggaactac ttcaagagta gtaaaaagcg caattgatat attaagagaa gaaggaataa
     6361 aagcaggtct tataagacct aaagtattat ggccattccc attcgaagca ttcaaccaaa
     6421 ttcctaatgc tagaaacata ttgactgttg aaatgagtat gggacaaatg gtggaagatg
     6481 ttaaaatggc agtagaaggt aaattaccag tttacttcca tggaagacca ggaggaatga
     6541 ctccaacacc agcagaaata gttgaaaaag ctaaaaaaat tattgccgga gaattggttg
     6601 caggaggtgc tagataatgg ctgttgtatt taagaaaact gaaggattac aagatactca
     6661 aactcactac tgtccaggat gtacacatgg tataatccat agattagttg gtgaagtatt
     6721 agaagaacta ggagtattag gtgatgctgt aggtgttgtt ccagttggat gttcagtttt
     6781 aggatataaa tattttaact gtgacactca agaagctgct catggtagag cgccagctgc
     6841 tgctacagga ataaaaagag ttcatcctga aaatacagta ttcacttatc aaggtgatgg
     6901 agacttagct tcaattggta ctgctgaaat agttcatgca gctgctagag gagaaaaaat
     6961 aactactata tttgtaaaca atacaactta tggtatgact ggtggacaaa tggctccaac
     7021 tacattagtt ggacaaagag ctactactgc tcaatcagga agaaatgctg aaactcaagg
     7081 ttatccaata agagtaagtg aaatgttagc tacattaaca ggagctgtat ttgtagaaag
     7141 agtagcagtt gatactccag ctcatgttag acaagctaaa aaagctataa agaaagcatt
     7201 ccaagttcaa caagcaggat taggatttgg aatagtagaa gtattatcta cttgtcctac
     7261 aaactgggga ctagctccaa acgatgcttt acaatggtta agagacaaca tgataccata
     7321 ctatccatta ggaaatttca aaaatgttga agttgaggag gtgaagtaaa tgtctacagc
     7381 tagagtaata tgtgcaggct tcggaggcca aggtgttatg tctatgggac aattacttac
     7441 ttatgcagga atgctagaag gaaaagaagt ttcttggtta ccatcttatg gaccagaaat
     7501 gcgtggaggt acagcaaact gtgcagtaac tgtatctaat gagccagttg gttctccact
     7561 tataacagat gatgcaactg cagctatatt attaaatata ccagcatttg aaaaattcaa
     7621 agatgatgtt attcctggtg gaaaaataat agtaaatagt tctttaataa aagaaaaagt
     7681 tgatagaaca gatgtagatg tttattatat accagcaaat gaattagcag ctgaattagg
     7741 aaatgataaa gttgctaaca tgataatgtt aggcgcttac ttaaaagtat ctgatactgt
     7801 agatatagaa tctgtactag aagcattcaa aaaagttttc ggacctagaa aagaaaaatt
     7861 cgttccatta aacagagaag ctttacaaaa aggtatggat gctacatgtt ctaaagcaac
     7921 taactaataa agaaaaagat gtcaaaaaaa agacaataga attatttcta ttgtcttttt
     7981 tgctgataag tattaaaata tttttatgac tgaaattaag cctataatga tattttatga
     8041 actataaaaa tataatacaa attacaagct tttcaattat gaaaggagaa tctttagtga
     8101 gaaagtattt tggaacagat ggagttagag gagtagcaaa tacagaacta acatgtgatt
     8161 tagcatataa acttggtaga gctggtggat ttgtcttagc tcaaggagac cacagagtaa
     8221 aagtagtagt aggtaaggat actagaatat caggtgatat gttggaagca tctttaatag
     8281 caggacttat gtctgttgga tgtgatgtta ttactgttgg aataatacca acacctgcag
     8341 tggcatattt aactagaaaa tatggtgctg attgtggtgt ggttatatct gcatctcaca
     8401 atcctgttga atataatgga atcaaattct ttaataagaa tggatataaa ttagatgatg
     8461 aaattgaatt gaaaatagaa gaatatatag acgatataga caaaattgat tgtcttccaa
     8521 ttggtgaaaa tgtaggaaga aaattacatg aacattgtgc acaaagagac tatgtagact
     8581 acctaaaatc aataataagc actgacttta agggattaaa agtagttttg gactgtgcta
     8641 atggtgcgtc atataaagtg gcaccaatag tgtttgatga actaggagca agtgtaattt
     8701 caataaatag ttcaccagat ggaaataata taaactataa atgtggctct acacatccag
     8761 aacaacttca aagggctgtt ttggaacata atgctgactt aggtcttgca tatgatggag
     8821 atgctgatag attaattgca gtaaatgaaa agggtcaaat tgttgatgga gaccatataa
     8881 tgatattgag tgccttaaat ttaaagaaaa ataataaatt ggcacaagat actttggtag
     8941 ttacagttat gagtaatata gggttgacaa tagctgctaa ggaaaatgga ataaatttat
     9001 ctactacagc agttggagac agatatgttt tagaagatat ggttaaaaat ggatataatc
     9061 ttggtggaga gcaatctgga catatgatat ttttagatta caacactact ggagatggag
     9121 tgcttagttc tctaatactt gcaaatataa tcttgcaaga aaagaaacct ttatcagaaa
     9181 ttgcatcaat aatgagccaa tacccacaag tattagtaaa tgcaacaata aaaaatgaaa
     9241 ataagaacaa atatatggaa tatccagaaa taaaaactga gatagaaaga atagaatcta
     9301 ttttagacgg aaatggtaga gttttgataa gaccatcagg aactgagcca ttagttagag
     9361 ttatgttaga gggtaaagag gaaggtcaaa taaaagaatt agcaacaaac ttagctaatc
     9421 ttatacaaga aaaattatcg taatttaaag ggtattctca gatataaata ctgaggacac
     9481 ctttctaagc gccagaactt agttatagac ataattattg agtaatatag gaactaagtt
     9541 gacgaggtca gagttaatcg aattgtcggc ggatgctctg cggtgtgttt accatcgaaa
     9601 gacttcttta aataatagga gcaatcctat ggacaaaggg aagccaaagt aaacttatat
     9661 gacctaactc ttaaaatata taaataatat ttttaggagg acacaaataa tgtgtggaat
     9721 agttggatat ttaggaagta gaaaggcagc agaagttata gtagaagggc tttctaagct
     9781 agagtacaga ggttatgact cagcaggagt agcagttaat agttctaatg aaaaagaact
     9841 taatattaga aagtttaaag gtagactttc tgtacttgca gaagacttag aaaagaatcc
     9901 tattgatgga aatttaggta tagggcatac gagatgggca actcatggag aaccatctga
     9961 tgtaaactca catcctcatt ttaatcaagc caaaacaata gcagttgttc ataatgggat
    10021 aatagaaaac tatatggaaa taaaagaaga attaatttct gaaggtgtta agtttgaatc
    10081 tcaaacagat acagaggtaa tagcacattt agtagataaa tactatgagg gtaatttatt
    10141 agatgctgta tacaaaacta tatcaaaatt aagaggagct tatgcattag gtgttatatg
    10201 taaagaacat ggaaatgaat tagttgcagt aagaaaagat agtcctcttg tagttggtgt
    10261 tggtgaaggt gaaaacttta tagcatcaga tatacctgca ttattaaaat atactagaga
    10321 tgtttatttc ttagaaaatg gagaagtagt tcatctaaaa gacgaaaatg taacagttta
    10381 tgactctaat agaaatcttg ttgaaaaaga agtatttcat gtaacttggg atgtagaagc
    10441 tgcgtctaaa ggtggatatg actactttat gtcaaaagaa attcatgagc aaccaacagg
    10501 agtaagagaa actttagaaa gaagacttga tgataatgga aatataattc tagacagtat
    10561 aaatatttct aaagaagact tagaaaaaat aaataaagtt tatatagtgg cttgtggaac
    10621 agcatataat gctggattac ttggtaagta tgccatagaa aaatttgtaa atataccagt
    10681 aataactgat atagcatcag aatttagata tagcgaccca tttgtagatg aaaattcatt
    10741 agtaatatta gttagtcaat ctggtgagac agcagatact ctagcagtat taagagattc
    10801 aaaggctaaa ggagcaagaa tactttcaat aactaatgtt gtaggttcat ctatagcaag
    10861 agaatcagat gatgtattct atacttgggc aggacctgaa gtagcagttg catcaactaa
    10921 ggcatatact actcaaataa cttctttata catgatagct ttagactttg ctatcaaaaa
    10981 aggaacaata actagagaat tttatgatag tatgatttct aagatgaaag aaataccatc
    11041 aaaaatacaa gaaatactag acaatgaaga atatataaaa gaagttgcta aaacagttgt
    11101 tagttctgaa catgcattct atttaggaag aggtatagac tatagcttag caatggaagg
    11161 ttcattgaaa ttaaaagaga tttcatatat acatgcagaa gcttttgcag caggagagct
    11221 aaagcatgga acaatagctt taatagaaaa aggaactcct gtaattgcca tagctactca
    11281 agaaaagtta tttgaaaaaa tggtttctaa tatggaagaa gtaagagcaa gaggagctta
    11341 tgtagtagca atagctcaaa gccataataa ggatgttgag aaagctgctg ataaaataat
    11401 ctatatacca aattcagatg atattttatc accaatatta gcagttgtac caatgcaatt
    11461 acttgcttat catgtctctg tactaagagg atgcgatgta gataagccaa gaaatctagc
    11521 taaatcagta acagttgaat aaaaacagat taaaaattaa atagataata tttattgaaa
    11581 agaactcatc ctgttatttg tattttatag gatgagtttt ttagataaat attaaatttg
    11641 ttttgttttt gctataataa attttataaa aagattgata ttaaatcatc tgtaaa
//
LOCUS       DAELTA010000115        11614 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.115, whole genome shotgun sequence.
ACCESSION   DAELTA010000115 DAELTA010000000
VERSION     DAELTA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.115"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..622
                     /locus_tag="KPG13_002648"
     CDS             <1..622
                     /locus_tag="KPG13_002648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986350.1"
                     /translation="SMVKKNETVGKDTYLSNIMKQKQELNDRIRDLKYRQEVYTKKIN
                     DAIKNLCKSEIRETTNNFSNIEIGIKNSIIEEKNKSTMLDENSTYLNTNDEKESLITK
                     ESNEKIEEEILNDEKLEELEQKKDYKEDSNKKEKVSEDLSLVGKTREELENMLKNFIN
                     LTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTDEQKKHINVLI"
     gene            800..1690
                     /locus_tag="KPG13_002649"
     CDS             800..1690
                     /locus_tag="KPG13_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986351.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2083..3039)
                     /locus_tag="KPG13_002650"
     CDS             complement(2083..3039)
                     /locus_tag="KPG13_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG0986352.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3742..4737
                     /locus_tag="KPG13_002651"
     CDS             3742..4737
                     /locus_tag="KPG13_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0986353.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4715..5578
                     /locus_tag="KPG13_002652"
     CDS             4715..5578
                     /locus_tag="KPG13_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0986354.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5575..6369
                     /locus_tag="KPG13_002653"
     CDS             5575..6369
                     /locus_tag="KPG13_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG0986355.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6654..8033
                     /locus_tag="KPG13_002654"
     CDS             6654..8033
                     /locus_tag="KPG13_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0986356.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8481..9194
                     /locus_tag="KPG13_002655"
     CDS             8481..9194
                     /locus_tag="KPG13_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986357.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9466..11004)
                     /locus_tag="KPG13_002656"
     CDS             complement(9466..11004)
                     /locus_tag="KPG13_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0986358.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4218 a         1331 c         1776 g         4289 t
ORIGIN      
        1 aagtatggtt aagaaaaatg aaactgtagg aaaagacact tatttatcta atattatgaa
       61 acaaaagcaa gaacttaatg atagaattag agatttaaaa tatagacaag aggtttatac
      121 taaaaaaata aatgacgcaa ttaagaactt atgtaaatca gaaataagag aaacaactaa
      181 taatttttct aatatagaaa taggtattaa aaatagcatt atagaagaga aaaataaaag
      241 tacaatgtta gatgaaaatt caacttatct aaatacaaat gatgaaaaag aatctttaat
      301 cactaaagag tctaatgaaa aaattgaaga agaaatatta aatgatgaaa aattagaaga
      361 gttagaacaa aaaaaggatt ataaagagga ttctaataaa aaagagaaag tatctgagga
      421 cttatcttta gtaggtaaaa ctcgtgaaga gcttgaaaat atgcttaaaa attttataaa
      481 tttaacacaa gaagaaataa tgaaacttga gtcgagaata gaaaagttag ataaaaatgc
      541 tgaagaatac aaacaaaatt caaagactaa tatatttgat aaaacagatg aacaaaaaaa
      601 acatataaat gtactgattt aaacaatatg ttttaaaaaa ataagtttat tatataatga
      661 aaatataaag gtacaatttc aatacacttc tattaaacca ataatttatg aaccagactt
      721 aatttcaatt tactaataac catatattta tgagttaata gttttatgtt ttaaaaagaa
      781 ttaaataagg gggagtagta tgaagaagaa tatattaact actatgatta ccatgattat
      841 tatattgatg atgagtatga cagtaatggc agagccatat caagaagatg aaacatctag
      901 cttgaaaaaa cagagtgcaa atgaacgtaa tcagactaat ataggcacaa tggatggcag
      961 agtgcaaaca aatgcaataa gtaaaggtgc taattatgac cctaaatatc ctcttaaagg
     1021 aatgttaaca caacttggtc ttaatacaac aggtaaagag ggatgggatt tactctatgg
     1081 aggaaatccc aatggagttg agcttcgagc agatggtaaa tattattata taaataaggg
     1141 tatggaaggg ctaggatttt atgcggcagc attgtcaggt ggctcatatc agagaatagg
     1201 ctttggaaaa cttgacccta tagaagtaat agctgatgga aattggattt cttacaaaca
     1261 agctcaagat acacttacag taatcaggaa ttttttaaat agctttgact ggagaaatgc
     1321 atctgagatg gaaagagcta atagagctgc aaaattggta accgaagcaa aatatgcaga
     1381 ctctaaatat tgtaacatag tctatggtaa tctggttgat aaacgtggtg tatgtggaag
     1441 ttttgcaagt agctttcatt tattaacaag gttaatggga atggattcac tatctatatt
     1501 aaatccatca ttaaatcatg cttggaacta tatacaaatt gatgggaaat ggtataggtt
     1561 tgatgggtct gaaatatcag cttttggtgg atcattagac tttgattacc gtaaattaaa
     1621 ggatgcaaca agagagatga ctacttacta tgatgctaag gcattgtcta tattaggatt
     1681 taatcaataa aaataggggg aggcttctta tagagccttt ttttattgat ttgaaattta
     1741 ataaggaatt ttatatgaaa atttaatcaa taaaagcaaa aaaggctctt tataaagcct
     1801 ttctaagtgt ttttttaagt aaaatttttt atatcaacat cagttttaat caaaacatat
     1861 aacattttgg acttactttt aaaaataaaa ttattaaaat ataatattaa tcatgttttt
     1921 agtttattat attctataat agtggtcatg ttgactattg tagttttatt aagaatagtt
     1981 ggtttatata aatatatgca aagatagctt gttgtttact ttacttatta gtatatagga
     2041 caagatactc taataaattt gcctatttta atatcttttg tactaaaacc aactaaagtt
     2101 tcctttatta tttcgttttg aataatacaa agcataatcc gattgtttgt ataaagactg
     2161 gaaagtatca gagttatttt ctaatttaga tatacctata gaacaagaat tactctctag
     2221 gcataattct aaaaccttct ttgccttatt ttcaacattc tcttttgatt gattactata
     2281 ttcaaaaaca gcaaattcat ctccaccaag tcttccaata atagcatcat ttacgaatac
     2341 tttagtcaaa atatttgaaa aatgtattag tatttcatct cctcgaacat gaccaaagat
     2401 atcatttacc tgtttaaaat tatctaaatc aaagatgtaa aagtatccat gtgaaacagt
     2461 tttaagcatt tcttttatgt gtacttcaaa aaaatgtcta ttgtatagtc cagtcataag
     2521 gtctttttct gattttagtt taaattcatt tgccattgct agtgcttctt gtgctttttc
     2581 agttattgtt ttagggtaat actcatcttt catttggtca aagtttggaa tagaatggtg
     2641 aacatggata agtttaatca tatcatctgt aactctgtat accatagtaa atcgagtatc
     2701 catttctaca tagatatctt tttctaggta atctttttct attacccaaa gagtaccata
     2761 tactataaaa gcttcgtctg tgactttggt acatgaatac cactcatcta taatctcaaa
     2821 gtctatttgc tctgattctg tttggtcttt ttcaagacct ataataaatt cttctaatgt
     2881 ttcgtaaaat tcatgttttc ctgttccaat taatgcaagg ttgtcatcta ataaggaaat
     2941 aataaattta aggttttctt cttctggatt taaaatataa atatgccaaa ttttttttga
     3001 taaatctctt aattgttttt gtattgtatt attttccata gattaccccc ttaagattta
     3061 cagtttatat tttaatgtat tataagttga actcttatta caatattatt ataatataga
     3121 actaaagaaa ttacatcctg tataggtaaa agtatatctt ttacaataaa aatattacag
     3181 taaaatgacc agttttaata tacgtattat atttttaatt tataaacgaa tattataaat
     3241 gaaatttaaa ttcaaccttg aagaaataag ctaaatgtat tagtatatta atccatgctt
     3301 gaacataaat aacctttatg atatgatatc aataataata ttatgagagg agtgccttga
     3361 tgtcggagtt atataatggt aaccaacaat taaatagttg gagacacata tattctgtat
     3421 aatcagaaat ttcaagtgta tttttaatac atttaatttt tgtgtcttat tctgccttta
     3481 tagataactg acatatgaca acaaatagta tttaaaatat atattacttt gttaaattat
     3541 ggtgtagtag gctttaatct gctacacttt tattaatttt gtatagtatt attttgataa
     3601 ctattttgaa atagagagca ttaatttttc tagtccacag gagttttctg tttttgtgct
     3661 gttaaatata agcacagaaa aaggcagata tatacctgtg gacttttttt atataaatta
     3721 tgaaatatgg aggtagttaa aatgagtata attgaactta atgaactgac taaagcttat
     3781 agaagatttg agaaaaaggc aggatttatg gggagtgtaa agtctttatt taataggaaa
     3841 tatacagaaa aaacagctat attgtcattt gatttaaaga tagaagaagg agaatttgtt
     3901 ggattaatag gtcaaaatgg agctggtaaa actactctta ttaagatgtt aactggtatt
     3961 attgcaccgt cttctggaga aatatctgta atgggttatt accctaacaa attagaaaat
     4021 aattttaaaa aacaatatgc tgttgttatg ggccaaaaaa gtcaactatt ctttgaattg
     4081 acaccagcag atacgtttct tttatttaaa gaattatatg atatatctaa tgaagaatat
     4141 aaaaaaaact tggattactt tatcgaatta tttgaggtat ctgactattt aaatgttcaa
     4201 gttaggacgc tttctttagg agaaagaatg aaaatggaac taatagtagc ccttctacat
     4261 aatccaaaaa tattattttt ggatgaacca actataggct tagatgctat agcccaaaaa
     4321 caaattaggt atttcctaaa agaagtaaat gaaacaaaag gaactactat tatacttact
     4381 tctcattaca tggaagatat taagagtctc tgtagtagaa gtattgttgt agaaaatagc
     4441 cgtaaaatat atgatggttc tacagagaaa ctatttaatc attttcaaaa atataaaaaa
     4501 ataacagtta gtttttccaa tgaaactaac attgtatttc caaaagattg taaagttgtt
     4561 gaacaaacaa atcataagat atcagtaatt gtgccaaaag aaagaggaaa tgaaacgatt
     4621 aaatggattt ttgataaata tgatgttaaa gatgtaatga ttgaagaaga agatataggt
     4681 agtattgtag agagaatata taagaaagga agtgatgagt attatgagca actataatga
     4741 agcaagttca aatgattcaa tgttttataa atttaaacag agtattagaa ggtattatga
     4801 agtagccaaa attaacttta aagtgcaact tatctggaga tttgatgtgg tggttaatat
     4861 ggtgtttacg attgtaaaaa ttttatttgc ttatatttta tggaaagcaa tttttggtga
     4921 aaataaaata gtctctggtt ttacatttaa ctctatgttg tcttactata taataagctc
     4981 atttttagca ggtattgata tgtcaaagag taccagtgaa gagatatgta atcgtgtaaa
     5041 attgggcacc ttttcaaagt acatgattat tccaattaat gttcaaaatt attttattgc
     5101 taaaaatttt ggaatgacat gtttttattt tgtatttaat atgatttctg catttgtatg
     5161 gatttttata tttaaaatag attttgcctt tacatcaaat ccagttcaaa tattaatttc
     5221 aattttgatg atttttctag ggtttatttt tatgatgaaa ctccacttct ttattgggat
     5281 tttgtcattt aaatttcttg agattagttt ttttaaaatg ataattgaaa atattgttga
     5341 atttattaca ggaacaatga taccacttgt actcttacca gaaactatag taacagttat
     5401 gaaggtattt ccattttatt atacaacata tttgccatct atgttactaa taggtagaaa
     5461 cagcaatgag gcaatacaag gattaattat tctagcatta tgggttttat ttttttctgt
     5521 agtaaataaa gttatgtata aaagaatgag aacattattt gatggggtgg gtatatgatg
     5581 aaaaaaaatt tgaaaataat atttatgtta ttaaaaatga aattatcaaa gatgatggta
     5641 tttagattcg atttttttgg agcatttttt gtagatagtt cattatttat attacaactt
     5701 ctaatgttta atagtatata ttctcatgta gactcaatag ggggatggca acaaggagag
     5761 atgttaatat ttataggaac tttctcatta ataaatgcga taaatatgac gattttcttt
     5821 tttggtattt atgatattcc acgaaaaatc caagaaggag aattagacta ctatattaca
     5881 aaaccattag acccactatt taggttaaca tttgaaaata ttaatattgg ttcatcacca
     5941 ttaattctag caagtatact tattgtttta tatggaatat ctgagttaaa ctcagagata
     6001 actttagtta tgataataat atatattttc atggtgttat tgatgacatt acttttttat
     6061 gatgtatgta tcatacttag aacagtttca ttttttgtta tatcatcttc tgcgataatg
     6121 agattagaag ataatttatt gccaatgaat atgaaaattc ctggtgtaat atatgaaggt
     6181 gtttttaaag tattattcta tttaatctta ccatatggaa ttatgtcgac aattccgact
     6241 caaattttgg ctgggacaat aacaattaat ggattaatat actcaatttt aattgtattt
     6301 ttgtttacat tgtttatgtg gagattttgg aagttaggta tgagacatta taaaagtgca
     6361 agtagttaat aaattagtaa aaatagacaa tgtataacta tatataaata cgtttcatat
     6421 gacactaaac tatgtgaaca agtatttgcg ctaaaggcac tgaaagctat aaaattatta
     6481 aagtaaggat ttaaaagttt ctttagtaga aatataattg tgtaaaaaag caagattaat
     6541 taaaactata tccaaataat tagatataat tcttatgcag aaaggaggtc tgtaaatgtc
     6601 taattgtttg gaatatattt taaaaataat taatgtacag aaaaataaac aaaatgagtg
     6661 atgtatatta ttcagaaaat catgaacaag ataatcttta tgagcaagat aatttactgg
     6721 cacaaagatt taatttattt actctattga agtttgcatt tcctactatt tttatgatga
     6781 tttttatggg gttgtataca atcgtagata ctatatttgt atcaagattt attaatacaa
     6841 atgctctatc agcaattaat attgtatgtc caattattaa tcttatagtt ggaattggaa
     6901 ctatgcttgc cactggagga agtgctattg tagcacgtaa gatgggaaat ggtgatattg
     6961 agggagcaaa taaagctttt tctttaatcg tattatcagg agttgtaatt ggggtaataa
     7021 ttactggtat aggtattgta tttatggatt atatcattat atggttagga gcaagtgaag
     7081 ttttaattaa atattgtcgt tcatatcttt ttattatctt ggtttttgct tctgcaaata
     7141 tactacaagt attatttcaa tgtttatttg taacagctgg aaagcctcaa tttggattat
     7201 ggctagtgtt aggtgctggg tgtgctaata taattcttga ctatgtgttc attgttctgt
     7261 tacaaatggg tattagtgga gcagcacttg caactggagt aggataccta attcctaccg
     7321 tttcaggtat ggttttcttt tcaaaaaata aagggttact taaattttgt atacctagac
     7381 tagataaaag aatattattt gaagcttgtt ttaatggttc atctgaaatg gtgggtcaac
     7441 tatcaacagc agttactact tttttattta atgctatgat gatgaaatta attggtgaag
     7501 atggggttgc agctattaca ataattattt attctcaatt tcttttaact tcgctttata
     7561 taggtttctc aatgggtgtt gcaccaataa taagttataa ctatggaagt gaaaataata
     7621 cgcagttaaa tcatgttttt aagatttgta tattatttat ttcagtagca tctttattgg
     7681 tatttatctt ttctatatca tgttcaccaa tcattgtaga gattttttct caaagaggaa
     7741 gccgagttta tgaaattgca aggtctggat tttatatatt ttcttttagt tttatttttt
     7801 gtgggtataa tatttttact tcgtctatgt ttacagcatt atcaaatgga aaggtgtctg
     7861 caatattatc ttttctacgt acatttggat ttataactat aggtttattg atattgccaa
     7921 atatattaaa tataaatggg atatggttag cagtaccaag tgctgagatt ttaactttat
     7981 ttttaaccat tttttgtata tgtaagtata agaatagata taaatatatg tagatattaa
     8041 ataaatagtt taggtagata ttttatgttt taataagtaa ttcaattagt gggagatata
     8101 tactattgaa aattaaagtt ataattgata taaagaatta ataaaaataa catataaaag
     8161 tttaaaaggg agttgaactt aaagtttgat tagaaaaagt caattttaag acaactcctt
     8221 ttattttact attgtattaa tctgtatctg taagtgtatt agtctaatat tataaacttt
     8281 ggatgaaatt aattttctat aggttttaat gtatcataca ctttatttta aagaaactta
     8341 tcttaatttt catagtgatt gaatttgtct attagagatg ttactattag taatgtaaaa
     8401 gcaagagtag tgaatataaa aaacaagata gtgatactgc aagattagaa tataataaat
     8461 ttttatggag agatttcaat atggatacat gttttaataa aaaaatatta cttgttgatg
     8521 atgaaaagga tatagtagat ttaattgaag aagtactcat aaatgatggt tttaaaaaca
     8581 ttattaaagc ttataatggt ctagatgcta tttcgctgtg taagatagca tgtccagatg
     8641 ttgtcattct tgatataatg ttaccagata tagatggtat agaagtgtgt aaaaaaattc
     8701 gagagttttc atactgttct attctttttt tatcatcaaa aaatgatgat attgacaaaa
     8761 tactaggtct tagtagtggg ggagatgact atataacaaa gccattcagt ccaagagaaa
     8821 ttgcttttcg agttaaagcg caattacgta gacaacagta tcaaagcatt gtaccatcag
     8881 atagtgaagt tattaaaata ggagatatta ctattgatat agaaggaaac cgtgtatata
     8941 aagatagaaa tgagattgag ttaactggaa gggagtatca tcttttaagc tacatggctc
     9001 aaaatgtaaa taaaattata ggaaaagaaa gactctatga acaagtatgg ggagtatata
     9061 gtagtatttg tgacaataca attatggtgc atatacgaca tataagagaa aaaattgaag
     9121 ataacccctc aaatcctaaa atacttataa cagttaaagg actaggatat aaacttgtga
     9181 atagaatcga ttagaataga ttttaatatt aatgaagata tctcaagttg aatttttagt
     9241 taatgaggca ttcttttatg ggtaaaatca aatatatttt cgttatctca acaatgaaga
     9301 agaggtctat ctctattttg agatagcctc attaagttta tatcctggta atgtacttta
     9361 tcttctaata aaattttttt agtttatttg cagtgaaaca ttaactagaa cattaaaatt
     9421 tgcattgaaa tactaattaa ataatattgt atattgtgta taaacttata ctaattgttt
     9481 ttctatatta aatactttat caatccaacc tttataaaat ttttcttcat gggaaactag
     9541 gattatactt cctctaaact gtttcaaagc attttgtaaa gcatcctttg tctctgcatc
     9601 aaagtgattg gttggttcat ccaggataag aaaattacaa ggagataaca tcatacaaca
     9661 taatttaacc ttagattgtt caccaccact taaagtagat acactccttg aaacatgctc
     9721 ttccttcaca ccacatctgg ctagatgacg tctaatctct tttgtattta attttggata
     9781 tttatctgct actatttgaa gtggggtttt atcaggattt tcccatttta aatcttgctc
     9841 ataataacca attttaactt gttctgaaaa ctggaaatct ccagatatac gaggaatatc
     9901 tttgactaat gtctttaaca aggtagattt accgattcca ttaaaaccag ttataactat
     9961 tttttgacct ccatcaacag agaaatttaa ttttggaagt aaagaatagt agtatccaac
    10021 ttcaagattt gttatagtaa gagcattttg agccgatatt tcaatttctg aaaattgaat
    10081 atttggtttt cctgtaaaac taggaggagc tattctttct attttatcca attgtttttg
    10141 tcttcctctt gcaatttttg aatttactcc agctttattt ttacgtataa atgtttcttc
    10201 tttttctatt ttcttttgtt gtgcttgata ccttctaata taatcttctc ttaaatgtga
    10261 tttttgttta aggaactctg aatattttcc atgatatttc ttaatcatgc caaattctat
    10321 atcaagaata cctgtagaaa ttttttctaa aaagtcaaaa tcgtgggaaa ctacaataaa
    10381 tgccccatta aatgtattta aataatttga gagccattca acatgttctt tatctaaaaa
    10441 gtttgttggt tcatctaata atagaacatc atggttagaa agtagtaatt ttgctaaaat
    10501 tacctttgcc ctttgaccac cactaagctc tccaacaaca cgattcattc ctattgaatc
    10561 taatccaaga ccatttgcta ttttatttat ttcattatct atagaataga aattacttgc
    10621 tattagttgt tcctgataat ctgatgcttt gattagatat tgctcatttt cagatatagc
    10681 acttttttga tagagtaaat tcatttcctt ttctatttta taaagttctt caaatgcagt
    10741 gtgtaaatag agtgatattg ttgtatctct attgatttct gcatattggt ctaaatggcc
    10801 tatattgata ttaggttgcc acttgatttc tcctgaatct ggaacaactt ctcccaataa
    10861 aattttaatt aatgtacttt tacctgtacc attttggcca actacaccta tatgttcacc
    10921 tttgaataaa tcaaagttag ctttctcata cagtgattta tccataaaac attgtgataa
    10981 attagttact tttaataaac tcataattca aactcctttt cttttattaa aaaatttatg
    11041 gaaataaaaa aagcagagga atggcattgt aaaatgccga cctctgcttt tggaaaaaca
    11101 aaaggtcata gacaagcatt gttgtctatg accagaaaat ctatattatc tatcggcaaa
    11161 taggtaattt taaataagca tagccaaaaa actatactaa acctatattt gccgatgaag
    11221 atagaaagct ttaagctttc agattaacct ctacacaacg cttcatcgta tacatttgtg
    11281 tagagttaag ttttctaaca agtttaacta attctatata aatcataatt attcacatcc
    11341 taatcttagt attaattcta gtttatctgt atttaaattg tacttttgta ctttgtggat
    11401 tatcttattt atttaaaata tgttttatta attatagctt aagttatgtt atcataatac
    11461 ttagttatag tcaataagaa agttatattt actattatta tattaattta gtaaatctat
    11521 cattacaaag ttatttgaag actcgctatc aataaaattt tatagtctat aagaaaatta
    11581 agattatgag ttttataatt ttgatatagg gata
//
LOCUS       DAELTA010000116        11543 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.116, whole genome shotgun sequence.
ACCESSION   DAELTA010000116 DAELTA010000000
VERSION     DAELTA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.116"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(19..921)
                     /locus_tag="KPG13_002657"
     CDS             complement(19..921)
                     /locus_tag="KPG13_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0986359.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            1276..2454
                     /locus_tag="KPG13_002658"
     CDS             1276..2454
                     /locus_tag="KPG13_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG0986360.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            2463..3119
                     /locus_tag="KPG13_002659"
     CDS             2463..3119
                     /locus_tag="KPG13_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG0986361.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            3119..3784
                     /locus_tag="KPG13_002660"
     CDS             3119..3784
                     /locus_tag="KPG13_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG0986362.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            3889..4665
                     /locus_tag="KPG13_002661"
     CDS             3889..4665
                     /locus_tag="KPG13_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG0986363.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            4727..6061
                     /locus_tag="KPG13_002662"
     CDS             4727..6061
                     /locus_tag="KPG13_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG0986364.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            6557..7489
                     /locus_tag="KPG13_002663"
     CDS             6557..7489
                     /locus_tag="KPG13_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG0986365.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(7881..11420)
                     /gene="nifJ"
                     /locus_tag="KPG13_002664"
     CDS             complement(7881..11420)
                     /gene="nifJ"
                     /locus_tag="KPG13_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG0986366.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         3976 a         1868 c         1770 g         3929 t
ORIGIN      
        1 gttattttaa taacattttt aagagttctt tttactaaag aaatctataa taaatttaaa
       61 tacatgttgg tctacatggc tataacgttc ttttttaggt ctacataata taactttcca
      121 tgtaatagga tgattaaaag gcttttctat aattttgcta ttttcaaaaa aatcatgaac
      181 tggtgatggt aaaattgtta taaaatcaga atttgtggtt gatagcaaca aaaaatccca
      241 tgaaccagac attatatatc tttttaactt aacattttca tcagtaaatt tttgcattag
      301 cttatgatgt atcataaaag taggattaaa tattgcaagt aactgctcat tcaaatcgtt
      361 ccaatctatc ttatccttct tagcaagtgg atgatttata tttaaaaatg ctgtaagttc
      421 atcttcttga agcacatgtt cagttactac acttttatca attttatgtg gatgtaacaa
      481 tactgcataa tctaattcct gtaagataag cattctattt aagtcatatg cacccttctc
      541 tactatatca aattctatat ctggattatt tgcaactaac tgtgcaacta catctgaaaa
      601 tgctattcca agtataagtg gaggtattcc tattctaact tttcctttaa attgaactga
      661 atcttctctt aaatcttcca tcatattttt gtattcattt atgatatgtt ctgcatttac
      721 ataaaatctt tctcctgctg gtgttaaccc atttaacctg cctttatacc tttcaaataa
      781 atatacatct tcactttctt caaaattttt tataatttga cttaatgctg gttgagatat
      841 gtgaagtgtc ttagatgcta ttgataaatt aaaaccagat ttcactattt caacaaaata
      901 tttcaactgt tttatatcca ttttaaaccc ccttccccaa aagtataatt tagattatat
      961 tttttcttct aatgatattt tataggaaaa tcaacataat ttcattaata ttttaacaaa
     1021 atatttttct aaatctacta taaattttta tcctataaat ttttcttata cactatataa
     1081 aaaattagac ttagacaaaa cgcttttttc gccgtaatct ataattagaa acaggaaaac
     1141 ctaaagcatt aggaataaaa gtaattgaaa tacttaaaaa caagcttgta ttaaaaaagt
     1201 actttattat agtgttccct aatttcttta ttcagaaaat ttttaattat cattcaagaa
     1261 aggtaactat tagatatgaa agatgtagta atcgtatcag cagtcagaac acctatagga
     1321 agttttggag gtgtatttaa aaatacttca gctgttcaac ttggaactat cgctgttaaa
     1381 gaagcgatat cacgtgtaag acttaattta tctgaaatag atgaagtaat aattggaaat
     1441 gttcttcaaa cagggcttgg acagaatgtt gctagacaaa ttgctataaa tgcaggtata
     1501 ccaaattctg ttccaagcta cacagtaaat aaattatgtg gttcagggct taaaagtgtc
     1561 cagctagctg cccaatcaat cacatctggt gaaaatgatg tggtaattgc aggtggtact
     1621 gaaaatatga gccaagctcc ttatatcgtt ccaactgctc gctttggtag taaaatgggt
     1681 gacataacca tggtagatag tatgctaaca gatggcctta ttgatgcttt caatcagtat
     1741 catatgggaa taactgcaga aaatattgca actaaatttg aatttactag agaaatgcaa
     1801 gataaattgg cattagaaag tcaaaataag gctgagaatg ctataaaaaa caaccgcttt
     1861 aaagaagaaa tcgttccagt agatgtttta atacgtcgag gaaaaataga aactattgat
     1921 aaagatgaat atcctaaatt aggtatgaca tttgaaggtt tatctaaact taaacctgca
     1981 ttcaaaaaag atggtactgt aacagctgga aatgcctcag gtattaatga tggtgcggct
     2041 atgttaatac taatgagcca acaaaaagca gacgaactag gtattagacc acttgctaaa
     2101 ataaaatcct atgcttcagc tggtgttgaa cctgaagtca tgggtactgg tccaatccct
     2161 gcaactagaa aagcactgaa aaaagctgga cttagcatca atgatataga cttaatagaa
     2221 gcaaatgaag cgtttgctgc ccaagcatta gctgttaaaa atgaacttca aatagattca
     2281 tcaaagttaa atgttaatgg tggtgcaata gcattaggac atccaatagg agcaagtgga
     2341 gctagaatac ttgttacctt aatttatgaa atgcaaaaac gtaaagttga aactggactt
     2401 gcaacacttt gtattggtgg tggtcaagga atatcaatgg tggtttcacg ataggaggaa
     2461 atatgaataa aatagttagc attgatgaag ctctatctca tgtaaaagat ggagatacaa
     2521 taatggttgg tggattcatg gccaatggtt caccagaaaa tttaattgat tatttatgta
     2581 gtaaaaatat aaaagatttg acactaattt gcaatgatac tggctttatt gacaaaggag
     2641 ttggcaaaat ggttgtaaat aagcaattta aaaagataat tgcttctcat gtaggattaa
     2701 ataaagaaac tggaagacaa atgaataaca atgaaacaga tgttgagtta gttcctcagg
     2761 gaactcttgc tgaacaaata cgtgcagctg gttatggtct aggaggtata ctcacaccga
     2821 ctggtattgg tactcttgtt gaggagggaa agcaaaaaat tgttgtcgat agcaaagaat
     2881 accttcttga aaaaccaatt tatgcagatg ttgctcttct atttgcatca aaggtcgata
     2941 aagcaggtaa tctagtttat aaaggctcta tgaacaattt taataattta atggcatctg
     3001 ctgccaaaat tacaatagtt gaagcagatg aaatcgttga aattggtgat attgacccaa
     3061 atgaagttaa tacaccaggt atttttgtga attacatcgt tgaaggaggt aatttataat
     3121 ggataaatta gaaatgcaag aatatattgc aaatagagta tctaaagaat taaaagacgg
     3181 agccgttgta aaccttggaa taggtctacc aaccaaagta gcaaattata ttccggataa
     3241 tgtaaacgtt atcttacaat ctgaaaatgg ctttttaggt ttaggttcag ccgaagatgg
     3301 aaattcaagt gatgaaacta ttgtaaatgc aggtggtcaa ccagttacta tacttcctgg
     3361 aggatgtttc tttgatagtg ctacttcttt tggaataatc agaggaggtc atgttgatat
     3421 aactgttctt ggcgcacttc aggttgacaa atatggcaat atagcaaact atatgattcc
     3481 aggtaagatg gttccaggta tgggtggagc tatggaccta gtaactggag ctaaaaaagt
     3541 tattgtagca atggaacata cttcaaaagg ttctgcaaaa atattaaata attgtactct
     3601 tccacttact gcaacaaatg ctgtagattt gatagttact gaaatgggag ttatggaagt
     3661 aacatcagat ggaattttac ttaaagaaat aaatccagct ttcacattag atgatgttat
     3721 ttctgctact gaagctcctt taatattgtc agattcatta aatggaaaaa tatctgttgt
     3781 ttaaaataat taaaactaga aaataaaaat ctcttagacg tattacatat aaatttgtat
     3841 tgcttaaaat attaatataa aaataaaata aaattggagg aaatgaaaat ggttaaagat
     3901 aaagttgtat tcgtaacagg agctgcaagt ggaataggta aacaaatagc tgaatcgttc
     3961 ttaaaaaatg gtgctaaagt aatgttctct gatataaatc aagaagcttt aaataatacc
     4021 ataacagagt tacaaaaaaa aggttatgat tgcatgagtg taaagtgtga tgtaactaag
     4081 gaagaagaaa ttaaccacgc tatagacaaa actgttgaaa agtatggcag attagatata
     4141 ttaataaaca atgctggact tcaacatgtt tctatgatag aagatttccc aactgataaa
     4201 tttgaattta tgataaaaat aatgctgaca gcacctttta tagctactaa aaaagcattt
     4261 cctataatga aaaaacaagg ctttggtcgt ataataaata tggcctcaat caatggtgta
     4321 ataggatttg ctggaaaggc tgcttataac tcagcaaaac atggacttat tggactaaca
     4381 aaagttgctg ctcttgaagc tgccgattct ggtataacag ttaatgcttt atgccctgga
     4441 tatgtagata ctccacttgt cagaggtcag ttcaatgacc tttcaaaaac tcgtaatata
     4501 ccattggaaa atgtcttaga ggaagtttta tacccacttg taccacaaaa acgcctatta
     4561 gatgttcaag aaattgccga ctatgctatg ttcttagcaa gtgataatgc taagggtatt
     4621 acaggacaag cttgcttact agatggagga tatactgcac aatagttatt atatttaggg
     4681 cttgaagaaa gtattcttca ggtccttcta ctaaggaggg atatttatga gtattatcgc
     4741 gccaattggt ataattattg gtatcatagc tattatatat ttttcagtta aagaaattca
     4801 tataactata gctgctccaa tagcaacttt aatagttgtt attttaaata aaatggatgt
     4861 tgtaacgtca atgctaggtg ctgacaaaaa caattatatg ggtgcacttg gaaactatat
     4921 aatgagttat tttgcaatat tcttattagg ctcaatactt gctaaattta tggaagaaag
     4981 tggagctact gtatcaatag ctgattttat tttaaataaa tttggctcta atcatcctta
     5041 tagagtttta gttgctatat ttatagtaag ttttatatta acgtatggtg gtataagctt
     5101 atttgtagtt atgtttgcgg tcattccact tgcaagaact ctatttaaaa aacttgatat
     5161 ttcttggaac cttattcaga taccactttg gcttggtatt gcaacaatta ctatgactat
     5221 aattcctgga acaccagcca ttcaaaatgt tatacctatt caatatctaa atacgtctct
     5281 tactgcagct actataccaa gtattgtagg tagtataggt tgtgctacat ttggacttgt
     5341 ctatatgaaa tatgctctaa acaaaagtat tagaaaaggt gaaacttatg caacttacac
     5401 ttcagaatta gaagaagaat ctttagatag agaattacca ggattttttg caagtatttt
     5461 tcctctatta gcactcgtaa ttatagctat aactggaagt acttttggtt cagaatttat
     5521 gaaaaaaaat gtaatttaca tagctttaat agttgcaatt cttttatctg cttttttatt
     5581 ccataaattt ctatcatcta agatacagac attgagcata ggtgctagtg gttctatagg
     5641 tcctatattt gctacatctt ctgcagttgc atttggagca gtaataatgg ctgcacctgg
     5701 ttttgatgta tttagcaaac ttataatgtc aataccaggt agtccactta taagtcttac
     5761 tgttttgact tcaacaatgt cagcaataac agggtcctct tctggtgcat taggaatagt
     5821 aatgccaaac tttgcagatt attatttaag tactggtcta aatccagaat taattcaccg
     5881 tgttgctaca atagcttcaa atatactaac tatagttcca caaagtggtg tttttcttac
     5941 attcttaagc ttatctaagt taacacataa aactggattt aaagaaactt tcattgtagt
     6001 tgcagttggt tcactaatag cagaaattat tgtaattaca ttaggattac taatgtatta
     6061 atataaaaca agttaaaatt cataatatga aaggtggtgt ctcaaagtga actttttact
     6121 taatgagaca ctctttttat atgaaataac atatgcctat catttaaaca ataaaaaagg
     6181 ccttatctct attttgagac agaccctttt tatttcttaa agatttaaat aattataatt
     6241 tgttgctaat acgaaagcat ctactgaata aatttaacca aataaaaaaa aatttatcat
     6301 aactataaat ttacgttatg gaattattat aaaaatatta ttttctctaa ctaagtaatt
     6361 ccatactata caggctttta atacaaattg tcaaacacac tgaaatatac ctatttattc
     6421 catatatatc ttttttacat atctttacag taaaaaaagt cttaatctta tgttagtata
     6481 gattcacggg taacccccta cactaaaggt tacaaatcag taacattttt agaattttta
     6541 gattaagagg tgtttaatga tgagaagaaa tataccaaca aaattaaaaa ctatattagc
     6601 tttaggagtg ttaactttag caacagcttc aagtgctgtt actgcaagtg cttcatcact
     6661 tgaaaataat gtaaatataa gtaacaaaag tttagtatat agtaatacta aatacaagtt
     6721 ttctgcaaaa ccaaattgtt ctgtaaaacc agaacttaag cctgataaag ataactcttg
     6781 taaggataag aatcataatg acaagaattg caacaacact aataaacctg aggacaacaa
     6841 taattcagac tctactaata agcctgataa caataaacct gagtctaata aacctgaaga
     6901 caacaataat tcaggctcta ctaataaacc taataataat aaacctgagt ctaataaacc
     6961 tgaagacaac aataattcag gctctactga taaacctgat aacaataaac ctgagtctaa
     7021 taaacctgaa gacaacaata acaataattc agactctact aacaaacctg atgataataa
     7081 caactcaggc tctacaagtg aaagtttttc agcttaccaa aaagaagttg ttgacttagt
     7141 aaatatagaa agagctaaag ctggattaaa tcctcttact cttgattctt ctatatcaaa
     7201 tgttgctaca aaaaaatctc aagatatgat tgataacaac tatttttcac acaattctcc
     7261 aacttatggt tctccatttg acatgttgaa aaaatttgga ataagctata agacagcagg
     7321 tgaaaatata gctatgggtc aaaaaactcc taaagaagtt gtaaacgcat ggatgaattc
     7381 tgaaggtcac agaaaaaata taatgaatcc aaatttcagt aaaataggtg ttggagtagc
     7441 acaaaaaagt ggtggttcaa tttattggac acaaatcttt gtaggataaa atactctttt
     7501 tccatacata tcaaaagagt atctctaagt gataaaaaac acaaatgaga tactcttttg
     7561 tatctatttt aaaattatca aatccaaata tacatactat aaatattatt tgttctctta
     7621 attttagtgt gtagaacaat atataaaaag tattggcaag atgaaaatgg aaaacttaaa
     7681 aaagatattt aataggtaaa aacaaaatct taatatcttc ataaaaaaag gctgtctcaa
     7741 aatagagata aggccttttt cattgttgaa atgattaaaa tatatttgat ttcatacaaa
     7801 aagagtgcct catgaactaa aaagctcact ttgagacact ctcttttatc aatttatata
     7861 agcattataa tactttaata ttattcagct aattttttgt atccttcata tctttcttta
     7921 gcatcagctt cagctttagc aaataatcca tcagcttctt ctgggaattg tttagctata
     7981 gcagaatatc ttacttgtgc aagtatgaaa tctttgaagc ttgcagttgg ttcttttgaa
     8041 tctaaagtga atgggttttt accttcagct tttacttctg gattgaatct gtataaatgc
     8101 cagtatccgc aagcaacagc ttgtgcttcg ttagctacag ttcttcccat accttctttt
     8161 aatccatggt ttatacatgg agcataagct attattagag atggcccatc atgtttttca
     8221 gcttctataa cggctttgat aaattggttt ttatcagcac ccatagcaac ttgagctaca
     8281 tatacgtttc catatgtcat agccatcatt cctaaatctt tctttctaga ttttttacct
     8341 gaagcagcaa acttagccat agcagcagct ggagtagctt tagaagcttg acctcctgta
     8401 tttgaatata cttctgtatc aaatacaagt acgtttacat cttcaccaga tgcaagaaca
     8461 tggtctaatc caccgtaacc gatatcatat gcccaaccgt ctccacctag tatccattga
     8521 gatctcttaa ctagataatc ttttctatct tttatagctt ttactaactc tttagctttt
     8581 tcatctgtac aatcacaagc tggtttttct aataattcaa caaccttagc acttgctact
     8641 ttagaagctt ctccatcatt tctagaatct aaccactctg taaatactgc tttagcatct
     8701 tcacagatac ccatagaaac taactctgtc atagcatcta ctattttatt tcttatttgc
     8761 ttaacagctg tgtacatacc aaatccatat tcagcattat cctcgaataa agagtttgcc
     8821 caagatggac ctttaccttc atggttacaa gtataaggaa ttgatggtgc agaagctccc
     8881 catatagaag aacatccagt agcattagct atcatcattc tatctccata taattgtgta
     8941 actactttaa tgtatgcagt ttctccacat ccagcacatg ctcctgaaaa ctccattaat
     9001 ggttgtctaa attgacttcc tttaacagtg tttacaggca taacatcacc ttttggagct
     9061 acctcatcag gatttactgc aaatgcccaa ttatctaact ctgtatcaag ttgagtatct
     9121 attggcttca taactaaagc tttttcttta gctggacata tgtcagcaca gtttccacat
     9181 cctgtacagt caagtggact aagttgcatt ctatatttta atccttctaa tcctttacct
     9241 aatgcttttt tagcttcaaa tccttctgga gcattagcta attcttcttc agtaactaat
     9301 actggtctta tacatgaatg aggacatata aaagaacatt ggttacattg aatacagtta
     9361 tctattatcc attcaggaac atcaacagct ataccacgtt tttcataagc tgcagtacca
     9421 caagggaatg ttccatcttc tataccattg aatgtactta ctggtaagtt gtttccttct
     9481 tgtgcagtca ttggtcttag tatatttttt ataaattctg gttctttaac ttctccttta
     9541 tctgcatcaa cagcattttc ccaagaagct ggaacttcaa ctttaactaa agcatttatt
     9601 cctgcatcaa cagcagcata gttcatgtta actatttttt cacctttttt accataagct
     9661 ttaactatag aatcttttaa gtatttaaca gcatcttctt ctggtattat ttcagctaat
     9721 ttaaagaatg cagattgcat tatcatattt attctgtttc caagacctat ttcttgtcct
     9781 agtttaacag cgttaactgt atagaatttt atatcatttt tagctatata ttgcttcata
     9841 tgagctggta aatgttcttc aagtcctgct tggtcccata tagtgttaag tacaaatgta
     9901 ccaccttttt taagaccttt taataaatca tattgataaa catatgattg tttatgacag
     9961 gcaatataat cagcttcatc tattaaataa gtagctctta ttggagtatc accaaatctt
    10021 aagtgagaca tagttatacc accagatttt ttagagtcat aatcaaagta agcttgagca
    10081 tatttttcag tatggtcacc aattattttg atagcttgtt tattagctcc aacagtaccg
    10141 tcagaaccta atccccagaa cttacatctt atagttcctt tagaagctat ttttattggc
    10201 tcagatggag ttaaagaagt attagttaca tcatcaacta tacctactgt aaatccattt
    10261 ttaggttgtt ctgaaactaa gttatcaaaa acagttttta tatctgaagg agtagtatct
    10321 tttgaaccta atccgtatct tccacctatt atcataggag cattttcttt tccatagaat
    10381 acatcacgta catctaagta taatggttca ccagttgaac ctggctcttt agttctatca
    10441 agtacacata ttctttcaac agtagatggc atagcatcta agaaatgttt cattgagaat
    10501 ggtctatata aatgaacttt aactaaacca tatttttctc ccttagcatt taagtaatct
    10561 atagtttctt ctattgtttc tgttactgaa cccatagcta ttaatacgta tttagcatct
    10621 tctgctccat agtaatcaaa taaactatgt tttctacctg ttaaattact cattttttcc
    10681 atgtattttt caactatacc tactatattt tgatagtatt tattagaagc ttctctagtt
    10741 tggaaatata tatctgggtt ttgagcagta ccacgagcaa ctggatgatt tggagataaa
    10801 gcattatctc taaatgcttg aactgcttca aagtttaata aacttgcata atcttcattt
    10861 tctagtaatt caactttttg gatttcatgt gaagttctga atccatcaaa gaaatgtata
    10921 aatggtaatc taccttctat tgcagctaaa tgtgcaacag gtgctatatc agctacttct
    10981 tgaactgaac cagaagctaa taatacacat ccagtttgtc tagcagccat aacgtcttgg
    11041 tgatccccaa atattgataa cgcttgagat gctaaagcac gagcacttac atggaatact
    11101 cctggtaata actctcctgc aactttatac atgttaggta tcattaataa taaaccttga
    11161 gatgctgtaa aagtagaagt taaagctcct ccttgtaaag aaccgtggaa tgttcctgat
    11221 gcacctgctt ctgactgcat ttcaacaaca ttaactgttt gtccaaatat attttttctt
    11281 ccttgtgatg cccactcatc aactacttca gccatagttg aagatggtgt gattggatat
    11341 atagctgcta catctgtaaa cgcataggca acatgagctg cagctgtatt tccatcaagt
    11401 gttttcataa acttagccat aatatgtatc ctccttgata gctacaattg tacttgttta
    11461 taacaagtct atttttttat atttacatat aatttacaca acaaaactat aaacacacat
    11521 atagtcgcgc aaatactcta tct
//
LOCUS       DAELTA010000117        11411 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.117, whole genome shotgun sequence.
ACCESSION   DAELTA010000117 DAELTA010000000
VERSION     DAELTA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.117"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            78..2621
                     /locus_tag="KPG13_002665"
     CDS             78..2621
                     /locus_tag="KPG13_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG0986367.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            2744..4735
                     /locus_tag="KPG13_002666"
     CDS             2744..4735
                     /locus_tag="KPG13_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986368.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            4725..5045
                     /locus_tag="KPG13_002667"
     CDS             4725..5045
                     /locus_tag="KPG13_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986369.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            5243..6112
                     /gene="galU"
                     /locus_tag="KPG13_002668"
     CDS             5243..6112
                     /gene="galU"
                     /locus_tag="KPG13_002668"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG0986370.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            6136..7149
                     /gene="galE"
                     /locus_tag="KPG13_002669"
     CDS             6136..7149
                     /gene="galE"
                     /locus_tag="KPG13_002669"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG0986371.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            7261..9195
                     /locus_tag="KPG13_002670"
     CDS             7261..9195
                     /locus_tag="KPG13_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG0986372.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            9350..10213
                     /locus_tag="KPG13_002671"
     CDS             9350..10213
                     /locus_tag="KPG13_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG0986373.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(10421..11302)
                     /locus_tag="KPG13_002672"
     CDS             complement(10421..11302)
                     /locus_tag="KPG13_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0986374.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
BASE COUNT         4840 a         1136 c         2036 g         3399 t
ORIGIN      
        1 ataattattt agatatcata atcaaattag caactttgca caatgtaaat attacaataa
       61 aataattgat ttaaggagtg aaggttttga gtgaaaataa tataataaga aacactgttt
      121 taaatgtcac agcaagtttt ttaaaacaag agtctaaaat aaatgaaaag ctagatggtg
      181 ttttggaaaa gaaatttgaa aaagtagaat ttaatgaagc aaaatatgca gagcttttaa
      241 aatttaatat attgttttat aagacattag caagaaatac agaaccactt attggcaaat
      301 ggatagtaga taagtacata cctgaaatag atgaattaga aaaagattta gagttaacta
      361 cagcaaaatg tagaaagtat gtaaataagg ctatgaaaga gggattagac tctctaaaag
      421 ccaatgattt aaatagtttt ttagcttatg ataaaatgga actatcagag agaagaagac
      481 gtttagaaaa ggattataag gttttaaacc tatataaaga ccttcttaat atatctctta
      541 gaaaaataag tttagaaaag aaagaatgtg gagatttatt tcttaaaaat caagcagatg
      601 cgaaaagaga attaaaaaga gaaattatat tctgtgtaaa caaaatatta gctagtaata
      661 aagatgtggt acctaataat gtggaagaga ataacgaaca aaacaaagta gaaataaaag
      721 caactgaaat tgaggatttg caattaggta aagataattc agaaaatgac atgctactaa
      781 atgaaattaa aaattcaaaa gtaaaacatg aatcaaaatc aaatgatata gaatcgaagt
      841 ttatagataa gttaaatagc ttaaataatg aaagtatatc tcaaacaata cacaatgaat
      901 ataagaagtt atgtggatta gaagaacttc attctgttga agcatatggt tttggcaaag
      961 aaattattaa agattttgct tgtgcaacag ttgtattaga atttttaaag agaagaaata
     1021 gagaccttat agaaggtgca atgagactga ctataattgg agaatttggt cctgaaaatt
     1081 ttaaggaatt tgtggactat gtaattaaaa ataaaacaga aattagtgaa gatacatgga
     1141 ataaagcaca gaatctaata aaagataact atagtgaatt agaaaatcat gaaattgcat
     1201 caaaaagaac tagaagaaat aaagatatcg atattgaaga atacatttac atgataaaaa
     1261 atgcagataa agatatttgc tttagaagta gtatttcaat agaagatgat gctgaagaag
     1321 ggactaaaga agaagtagat agtaataatc aagatatagg tgacgttgta gaagataaag
     1381 atactacaga taaagagtac gacagcaata aagaagatat aattgaacca gaaaataaaa
     1441 agagtaaaaa gaaggctaaa ttatttggat ttattaaaaa agacaatgaa gaagctgaac
     1501 aagaagaaga gaatttaaat gatatttctc cagatataat tttagataaa ccagttgaaa
     1561 atcatcaagt gaaatcagaa gaaatagagc aaaatgaatt gaaagaaata aaacaagaag
     1621 aaccaagtca acatatagaa gaagaacgta gtgtaaaaat agaaaaacca ataaataata
     1681 acttagatga aaaagtatct tcaaataatg aatcgaagct tgaaaaagaa agtaaaaact
     1741 tagaagataa aaaagcaaaa gaaataaaag aagaaaaatt agaaaatgaa aaatcagtag
     1801 taatacctat taaaaagaaa gaaaatagta ataaaaaatc taaaaattct tctaaggaca
     1861 aatatagaga aaacaagaaa gaaatgaaaa attatgtatc agataaagaa gattctttag
     1921 atgatgaaga ggttgtttct aagaagagta gattaaaaga aactattata gcagtagtta
     1981 tagtggttat tgttggtgta ggatatttta taacagtagg caacaataag aaaaatgata
     2041 aagagaatat tccaaagtca tcaactcaac aacaagctaa caataaatta actgaagaag
     2101 agaaaaaagc acaagctgaa aaagagaaaa aagaagcaga agaaaaggca aaagctgaaa
     2161 aagaggctca agaaaaagca caagctgaaa agaaagcaaa agaaatggaa gcatataaag
     2221 atggtaaagg tgtatattac actgtttatg caggttcact taaagttgaa aaaacagcaa
     2281 aagaaacagc aaaagaatat gaagctaaag gtatttcttc aacaataatt caagaaaatg
     2341 gatactataa aataaaaata ggtgattatt ctcaatatgg agaagcacaa gaaaaatgta
     2401 atgaactagc taagaagtct attgatacat atatagctat gtatgataag tactatgatt
     2461 ataagttaga agaattaaaa gaatcagctc cttctttgag tgctgaagaa cttaaacaaa
     2521 aatatgaaga tttaagaagt gagttaaaga acaaatctgg atatcgtgaa tatgttaaac
     2581 atttagataa actttatgaa gaaatagttg aaggtgctta gagcattata aaaaaggaga
     2641 aactatgtct cagaataaaa agtattaaaa aaaatattat ttgttttgag acattttatt
     2701 cacctttata aaatacttat aattctgttt aggtggtgaa ggtatgttga aattaggaga
     2761 aaaaatcata tatgagcttt cagatgggtt tgagcttagt ggcataagta gatactcaaa
     2821 agaatataaa aagcttcata taacaaatct agggaatata ataatcagca aatcagatga
     2881 agttaatgag gatacaggtg taaaatatat atatagtttt tcagacaata aagaaaaatt
     2941 agaggcagtt ttagaagatt ctcaaataat tgtctatgga aataacttgc ctttttacat
     3001 aaaaagtgaa aattcaagta aaaatgttac agcaaatttg actatgaaaa taactttgga
     3061 tgaatatgat ttaatctgca aaagtcaaag agaatttata ttctatctaa aggataatat
     3121 gactatatta agtttagatg acaataagtt ttatatgggt ggaattaata aagataatga
     3181 gaagtttata tttatcagtg ggaaaaatag atttgaaata aattttgatg atatagaaag
     3241 atacatttta gaagataaaa gagtgtcatt gaagggatac tttcatatgg aaagagaggg
     3301 gattatagta aggtctgtat ctatatttaa taacaatata gatagtgtag tcccatctga
     3361 tttaaatgag agagttaaag ataaccaaaa aataggaaat cttcctaaag attgtgaaat
     3421 agtattctgc aagatatcag gaaatataga tggatttgat tataaaaaca caaacatgct
     3481 tttagtaaaa tatcaagacc aattaatttt tataaataag aaatctaaga agactattgt
     3541 aaagtcagcg aaagataatt gttctaagtt aaatttagga gaagatataa tattatatga
     3601 taataaaaat gtatttaacc tacatataaa tgataaaaat agagaaatta tgcaaataga
     3661 tgacctaaaa gatatagaaa atgaaatagt gggatataca ttaaaacatg ctccattttt
     3721 tattcaagaa gatttcgata gcttgactat tctaaagtct tttcaaaaag aaatcatatc
     3781 tataaaaaat agtgatataa aagacattgt aattaataaa gaacttgaaa acgaaaattc
     3841 taattttgta gaaacggaaa taaaatttaa taatcaaaaa gtacttttaa acttaagtaa
     3901 aagtatggtt caaaaactta tgcaagatgt atttatatat gcaaagcaac ctctacttaa
     3961 agaaaattct attgaagtga tatataagaa ttggtctaaa gctatgaatg atatgataat
     4021 atttaatttc tttggaaata tttactatat gaaatctgaa tttgataaga ttttagaaaa
     4081 agagttaaat gatgaaataa gaatagaagt tataaattca ctttataagc aaatacaaga
     4141 acaaagaaac aatttagatt tattgtcagc atatatgcca agaatattag aaaatcaaga
     4201 aatagatttg tttgaaaagt ataatactaa acttgatgta caagtgttta agcagattaa
     4261 aaatctattg tctgatttat catataatat aagcagttat ttaaatgaag ttgaaaaaag
     4321 tttagacaat atcatctttg taatttcagg agaagataag aagaaataca attatagaat
     4381 gttaaaagaa agtgaaagtg caagtcttga tgtattttta aaacaagcca tatcaagatt
     4441 aaaccattta gtagaaaata tgtatcctta ttatgtagat gaaactagta gggaaatgtt
     4501 taagttattt gagttattgt ggaaaaacta tagaaatatt gatgatgata gtataaaaga
     4561 aatattattc gagagaataa caaatacata tgtatttaaa caattgacat taaataatag
     4621 tacaaaagaa agaagaaaag acataattga aaaaatatat aactctgttg attatggaac
     4681 caataaacta gatgaaaata tgtttttcac aggaggaata aagtatgtca agtaaataca
     4741 atgtttggac ttttaactat gagttcttag gattggactc aggggatgaa aaaaataatg
     4801 aagtttatta cgagattgat ttagatgaaa ttttaaatga aaactatata gatgaagata
     4861 tagatagtgg tttagattct gatggaaata gtattttagg agaattaata tcagaaatgg
     4921 atatagatga ggatgtcagt gttgatatga catatcaaga actagaagtt gacttagaag
     4981 atttagattc ttatctagat gaagatatag atagtgatat aaaaggcatc ttagatgaaa
     5041 tttagtacct gtataagttt tggataaaat ataaacaata caatattaac ttataaaact
     5101 aaaggtagat gaaatttcat ctaccttata ttatcttata gcattaaata ttgggataat
     5161 atagagtttg aagaaaaagt ataaatatgg tatattaatt tggtacaata gaaaatttac
     5221 gaagaagggt gaaaaaatgc agatgacagt taaaaaagca ataataccag cagcagggct
     5281 aggaactaga tttttaccag caacaaaatc acagcccaaa gaaatgcttc ctatagtaga
     5341 taaaccaact ttacagtata taatagaaga agctatagaa tcgggaattg aagaaattct
     5401 tataataaca ggtagaaata aaaaatctat agaagatcat tttgataaat cagttgaatt
     5461 agagttagaa ctagagcaaa aaggtaagac agagatgtta gaaatggtaa gagacatatc
     5521 aaatatggta aatattcatt atataagaca aaaagagcca aaaggtcttg gacatgcaat
     5581 ttactgtgca aaaagtttta taggtgatga accttttgcc gtattattag gtgatgatat
     5641 agtagattca gatgttccgt gcttaaaaca attaatagat acttataatg agtataaaac
     5701 aactgtatta ggtgttcaaa agatagctaa agaagatact aataaatatg gtatattgga
     5761 tgtaaaacat atcgaagata gagtatataa agttaaagat atggttgaaa aacctgcaat
     5821 tgaagaagct ccatctgata tagctatact tggaagatat ataataactc ctgcaatatt
     5881 tagcatatta gagaaacaga cacctggtaa aggtggagag atacaactga cagatgcact
     5941 tcaaactctt ggaaaacaag aggctattta tgcttataac tttgaaggta gaagatatga
     6001 tgtgggagat aaaattggtt ttttagaggc aacaatagac tttgctctaa agagagaaaa
     6061 tttaaaagat gatttaatgg actatatgag aaaaaaagta agtgaaaaat aaatattaga
     6121 aaagaggtaa ttacaatggc agttttagta gctggaggag caggatatat aggaagccat
     6181 acagcaatag aacttttaga atcaggttat gaagttgtta tagttgataa cttaagtaat
     6241 agcaactcaa tagtggttga tagaatcaag gaattatcta aaaaaccagt aaaattttat
     6301 aatattgata tcagaaataa agatgaaatg catgttgtat ttaaagaaaa taatattgaa
     6361 tcaattatac actttgcagc tttaaaagca gttggtgaat cagtagaaaa accaattgaa
     6421 tattacagca ataatctaat aagtacctta aatttatttg aattaatgag agaatatggt
     6481 gttaaaaagt ttgtatttag ttcttcagct actgtatatg gagacccaca tacttgtcct
     6541 atattagagg attttccact ttctgtaacc aatccatatg gaagaactaa acttatgata
     6601 gagcagatgt tagtagatat atcaaaagca gataagtcat tggatatagc tcttttaaga
     6661 tactttaatc cagtcggtgc tcataaaagt ggtagaatag gtgaagaacc aaatggagtt
     6721 ccgagcaatc taatgcctta tataacaaaa atagcagtag gtaaattgaa ggaattgagt
     6781 gtttatggta atgactatcc aacacatgat ggaactggag ttagagacta tatacatgta
     6841 ttagatttag ctgctggtca tgtaaaagca ttacaaaaat tagaagaaaa tccaggactt
     6901 gttgtttata atttaggaac tggaaagggg tatagtgttc ttgatttagt aaaagcattt
     6961 tctaaagcaa gtggaaaaga aataccatat aagattgtag gaagaagggc tggagatgta
     7021 gccatgtgtt atgcagattc ttcaaaggca gaaaaagagc ttggatggaa agctaagtat
     7081 gaacttgaag agatgtgtga agattcatgg agatggcaaa gtatgaatcc taatgggtat
     7141 gaagagtaga atacatactt atatgtataa atgtaacaaa tttgtaaata taaggtattg
     7201 gtaattttat gttataatta gacacatgta gtaatgtttc taaaagaggg ggaagttata
     7261 gtgaaaaaaa gtctaaaaaa gtatttagta ctagcactga ctttagtttt agtatttgca
     7321 tgtggcgtac cagaatcaag cgcagccagt aaacatgtga ttatagtaaa ttcaagaaaa
     7381 aatactctag gatattttgt aaataataaa cttgtaaaag aatttagagt tgcaacaggt
     7441 aaaaaggggt cagaaacacc aacagggaaa acaaaagtag ttaacaagat aaaaaataga
     7501 ccatattata aaggtaatat acctggagga agtccaagaa atccattagg agatagatgg
     7561 atgggattag cattaaaagg aacttatgga gatacatatg gaatacatgg aaataataat
     7621 gagagttcta taggaaagca tatttctggt ggatgtataa gaatgcacaa taaagatgtt
     7681 agatggttat ttgaccaagt tcctgtaggt tcagatgtaa ttatagatta tagtaacgac
     7741 agctatgtaa agatagcggc aaaatataag ataaatctaa atcaaacagg atggaaaaca
     7801 gaaaatggta agaagtatta tgtaaaatct gatggaacat accaaaagaa tagttggcta
     7861 aaagtaaatg gaaaaatgta ttactttgat gcttcaggag taatgcaaac aggatggaaa
     7921 acaattaata acaaaaaata ttatttagga actgatggag caagagtatc tggatggaaa
     7981 gtaatagacg gaaaaactta ttactttaac tcagatggag taatgcaaac aggatggcaa
     8041 gaaaagaatg gaaagaaata ctatctagga tctgatggtt tagcagtaac aggatggcaa
     8101 gaaatagatg gtaataagta ttactttgac aaaacaggaa taatgcagac aggatggcaa
     8161 caaatagatg gtaagtctta ctatttagac aaagatggaa agatgctaac tggttctcaa
     8221 aaaatagatg ggaaagatta tacttttaat gaagatggaa ctataaatcc tacatgggat
     8281 acaattatag gtgcaaatag atttgataca gctaaaaaga taagttcagt tggaaactgg
     8341 aatgctgata gttcagatac tgtaatatta gtaaatggta atgcaatagc tgatggtata
     8401 acagcaactc ctttagcaag tagctatgac tcaacaatac tacttactaa tacagctaat
     8461 ttaccaactg aaactgttga agaaatgaag ttattagcac ctaaaacagt aatcttgata
     8521 ggtggagaaa atgcaataag ttctaaatta gagcaagaaa taaaaactac tttcaacaca
     8581 gaaacaaaga gaatagcagg tcaagataga tatcaaactg ctacaagaat agctgaagaa
     8641 cttggaaatc gagaagaaat aaaaactgca tatatggttt ctggaaatgg ggaggcagat
     8701 gctttatctg tagcttctaa agctggggaa gaaaaacaac ctataatatt agtcaataaa
     8761 gatggaatta cagaagaatc ttataaatgg ctaacagaaa gaaaattaga aaatgcatac
     8821 tttataggtg gaccaagtgc aattaacgat tcagtaatag caaaaatgaa tgatataact
     8881 actgaagata tttcaggaaa tagaatatat ggagatagta gagtagatac taatgccaaa
     8941 gtgatagaaa aattctatgg agatactgat ttacaggctg tattagttag taaatcagat
     9001 gctttagtag atgctttatc agctggacca ttagctgtaa aattacattc accaatagtt
     9061 ttaatggata attcaggatt atcatcagag caacaaagag tatttgcaaa taaaaaagtt
     9121 gaaactcctt atcaaatagg aggcggagta tcttatatag taatggataa attaatggat
     9181 attcttgcaa aatagtaaaa atatatattg aaaaataatt ataattgaga tgtgaaattc
     9241 aatcaataaa agcttgattg ttttacatct caatttttta gtataaaatt aagtttaagt
     9301 tatataaaaa tcttggaata tttttaaaac tcatataaaa tgaatataga tgctatcatc
     9361 atatgttaag agggtgagta agattataaa gaaaaaatat ataatgttta ttgatgaaag
     9421 tggaagagct gatataaatc atgaagaccc atttacattg acaggagtaa tttttgaata
     9481 taaatatgca gtaagtcaag gcgattatgt atgttctttg aggaaagaaa tggatgcatt
     9541 taaagagtat tgttttggaa ccagtaacat aggaatacat ttgacagata tatcaagaaa
     9601 atcaagagca tttgagaagt ttgatgattc acagattaag ttattctatg atgagttgcc
     9661 tttatttcta tctagattag agtgtactat aatatcagta acagtagata aagctagatt
     9721 aaagaaatat tatgcacctt caaaggaacc gtatgttgtg gcttttatac atgttcttca
     9781 gaatttttat tcatttgtaa ataatgaaac tgtagaatct gcacgaatag ttatagagtc
     9841 aagggatgat gtctcaaact taaaagtaca aaaggcgttt tttgatgtat ttaataatgg
     9901 aacaatatac ttagatatag atgaagaatt aagagataag gttaagggtt ttataattgc
     9961 caaaaaagaa gatgctgact atagagaagg tttagaaata gcagatattt tatgtaatcc
    10021 tcttagcaga gcaagaagag ggttaataga agctaatcca aagtgtatgc aatatggaga
    10081 aaaaaacaaa atattcaaat ctgtcagaaa caaaatatat actccaacga gtgtgaatga
    10141 tataagaaat tggggattta aagaagttcc tattgtagag cctgttggac catggcttga
    10201 aaaaatgcgt tagatacaat tgacaaattt taaaaaatct gatataatgt aagtaaccta
    10261 gttattcagg aactgaatcc agttgctgtt gtataacgat aattactgag actcggatgc
    10321 cgactgtcag ttaggaatct atacttttaa aagtatagat ttttttattt tataaaaaga
    10381 ataaaagagg atatttaaaa ataacccctt tattaataaa ttataattga gcgtttttaa
    10441 tgacaaagtt tttaaagttt ttaattgcag gaggcaaata tctatctttt ataaatgcta
    10501 agtaaaagtt tctttcccaa cttgggtgaa taatctgaat tacttttact tttgtaaagc
    10561 taagcatatt catatttggg actacagcaa ttccgaaatt ttttgcaact aatccagcaa
    10621 ttacttggtc ttcctctact tcatagacta tattaggctg ttgatttatt tttttaaaca
    10681 tatcatcaat tatatatcta agaccacttc tctgattaaa tactatttga ggataaggta
    10741 tggtttcttt taaatctata gtatcctttg cagctagtgg atgactatat ggtacaatta
    10801 gtactaaatc ttgtttggca acaggtataa attcaattcc cttttctttt tcaagtttcg
    10861 aacaaaatgc aatatcatat tttctttctt taagaccttg aataatgtca gtagttacac
    10921 cagtatgaaa tttaaaatct attgatttag caggattaga ttttaaaaat ttgtgcacta
    10981 tgtctgggat aaaatcagta ccaagtgttc taagaaaagc taagtctatt tgtccttcac
    11041 cagtaccagt tatttttaat gattttacac ttgaatctaa aatttctaat gatttttcca
    11101 catcttttaa aaatattttt ccatacttag taaggactat atttctacct tctttttcaa
    11161 atagagccac cccaagttca ttttctagta aagaaatagc atgacttaag cttggttgtg
    11221 ttatagataa attttctgcc gcttttgtat agtgttctaa atgtgccaat gtaacaaaat
    11281 agcgtaagtg atataaattc aatttttgtt catcctttca taggtatttt tatttatata
    11341 tttgtagaag ttaaccttta agatattttt acatttttat tatatatgat tatagagtaa
    11401 gaataaaagc t
//
LOCUS       DAELTA010000118        11181 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.118, whole genome shotgun sequence.
ACCESSION   DAELTA010000118 DAELTA010000000
VERSION     DAELTA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.118"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(270..1166)
                     /locus_tag="KPG13_002673"
     CDS             complement(270..1166)
                     /locus_tag="KPG13_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0986375.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1307..2734
                     /locus_tag="KPG13_002674"
     CDS             1307..2734
                     /locus_tag="KPG13_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0986376.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2930..5524)
                     /gene="clpB"
                     /locus_tag="KPG13_002675"
     CDS             complement(2930..5524)
                     /gene="clpB"
                     /locus_tag="KPG13_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG0986377.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5561..6157)
                     /locus_tag="KPG13_002676"
     CDS             complement(5561..6157)
                     /locus_tag="KPG13_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986378.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6174..6578)
                     /locus_tag="KPG13_002677"
     CDS             complement(6174..6578)
                     /locus_tag="KPG13_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG0986379.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            6945..7319
                     /locus_tag="KPG13_002678"
     CDS             6945..7319
                     /locus_tag="KPG13_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986380.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7316..8179
                     /locus_tag="KPG13_002679"
     CDS             7316..8179
                     /locus_tag="KPG13_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986381.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8179..8817
                     /locus_tag="KPG13_002680"
     CDS             8179..8817
                     /locus_tag="KPG13_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0986382.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            9309..9722
                     /locus_tag="KPG13_002681"
     CDS             9309..9722
                     /locus_tag="KPG13_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0986383.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            9913..11130
                     /locus_tag="KPG13_002682"
     CDS             9913..11130
                     /locus_tag="KPG13_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG0986384.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
BASE COUNT         3858 a         1559 c         1365 g         4399 t
ORIGIN      
        1 tttctttgat taaatatctt tcagaatcta agccagatgt ctcaaaaaaa ctgatttata
       61 atataatcat tttttctgct attcttattt ttccaatttt agtttcatat taaaatatac
      121 ttttcataga cactacattc tttcatactc attttcacat ttaactatac aagtagtctt
      181 actcatttat gtactatact ttattaatat ttattaagaa aaactcaaag atttaaaaat
      241 atatattttc tataaagtat atttatgaat tatttcatat cttcttttaa aataacatta
      301 ctaacttctt tatgttcatt tctatactta gttggagata tattataatt ccttttaaat
      361 aagcgactaa aataatttat gctatcaaag ccacatctca tagcaatttc tgtcatattt
      421 aaatctgttt cctttaaata atatatagct ttatcaagcc tgattccatt tataaaatcg
      481 attgtagtct tacctgttat ttgcttaaat gttctacaaa agtgatgtgc actcaagttc
      541 acatgactag caagttcttt taatgtaatt ttatccatat atttttcttc tatcaaatca
      601 aagatacatt caaatttttc taaagaatac tttcttttta taaattcatc ttcagaatat
      661 attttatcaa tatgacttct taataagagt actattaatt gatagatatt agcttttata
      721 gataactcat atccaacttc tttattgtga tattcatcaa gtatagttgt aatacatttt
      781 aaaatcagag aatcattttg aattaaatta ttaaatacaa tatgatttaa agccaatggt
      841 gctaagtact tggtttgtaa caaatcaact tgattactaa aaaggaatgt gggttcaata
      901 cgaatactat agaattttaa attatctgat aagctttcta aataatgtaa ttcattactg
      961 tttgctatta caatatttcc aggcactacc ttaaaattat ccaatccaca ttcaagatat
     1021 gctataccat caataaaata atatatttgt agatgctcat gccaatgtct tttaaagttt
     1081 gctccttttt taatgtcatc ctttaaaaaa atatctataa agttttcttg acctttcctg
     1141 ttaaaatatg tatttttatc cttcattatt cccccctcat caaaatagtg ctaattataa
     1201 gcaatatagt tattgtattc attatttcat atcgctatta tataaaatgt aactatcatt
     1261 gtaaacatta acataatgca gtcattataa taaggaggta ataaaaatga caaactcttt
     1321 taaaaaagat gataatagaa aaaaaatcac agttttattt gtagtcattg ccatgacttt
     1381 tatggcaaca ttagattcta gtattataaa tgttgctctc ccagttcttg caagcaaact
     1441 aaatgtatct ctagcatcta ttgaatgggt aatagcaagt tactcaatta ttatttgttc
     1501 aacactatta ttttttggaa gacttggaga tattattggt aaatcaagag tatttcaagt
     1561 aggtactatt ctctttacat cagcttcatt actatgtgga ttaagtaatt ctttaacttt
     1621 acttatagtt tgccgcttta ttcaaggaat aggcgcttct gcctacatgg caaacaatca
     1681 tggaattatc acagaactat ttccaaaaga aagcagagga aaagccctag ggattttagt
     1741 tactgcagtt gctattggaa atatggttgg accatctgtg ggaggattta ttttgtcaat
     1801 atttgattgg aatgttatat tttttataaa tattccaatc ggtcttattg taatcttttt
     1861 aaatacaaaa tttcttccta atagtaaaaa atcttcagaa aatatggata aaactggtgc
     1921 tatacttcaa tttttaggaa caacattatt ttttagtgct ctaattagtg ctcaacaaac
     1981 aggattacta aatccatata tattaattgc actattatta tcaattattt ttattatatt
     2041 atttttgata ttagaaaaaa aacatcctca accgcttctt gaccttgaaa tttttagaaa
     2101 ttttaaattt tcattgaatt taatttgtgc tcttacatca ttcatttgta ttgcatcatc
     2161 aagtatttta attccatttt atttacaaag tactatgaaa cttcccccta ttcaagcagg
     2221 tttatttatg atactatcac ctttgatttt agccattttt tcacctatat ttgggaatat
     2281 atcagataaa attaagtcag aaaaaattat tttaattggt cttctcgtta tgagttttgg
     2341 attctttcta atgtcaagat tgaaagaaag ttctgccctt attttatttg taatttatat
     2401 atcgataata tcaataggac aagctatatt tcaacctgct aacaatgctt tgataatgtc
     2461 aagctgttct agaagtaagc taggagttgt aggaagcata aattcccttg taagaaattt
     2521 aggacaagtt ataggtatta ctatttctac aacactttta tataatttta tgagtattaa
     2581 agctggatat agggtcaatg attatgtgat taataatgat aaaatatttg tatttggaat
     2641 gagaaatgtt tatattattg ttacacttgt ttgcttaata ggtgcaatat taattggctt
     2701 ttatttattt aaatataata aaaatgaaca atagttttta taattatatt caaattatga
     2761 ttatattata agaaatttac taaaatactt cctttgagtt aaatttaatt tggacatatt
     2821 gacttaaaaa tgttttatta atttgataaa atatttcttt tcctatagtt tataaataaa
     2881 aatcagctga gcaaaatatt tagtatatta tgctcaactg attcttttat tatttaacta
     2941 aaacttcaat attttcatct tttccatcta taactatagt acatccatta tacacatctc
     3001 cagcaataag tttctttgca attatagttt ctaatgtatt tcctatatat ctcttaagag
     3061 gtcttgctcc atatacaggg tcatatcctt ctctcaccat aacatctttt gctgaatttg
     3121 taacttcaat agtaatatct ttttctttta atctattttt taaatctttc ataaatatat
     3181 ctataatttt cttgatattt tcttggtcta aaggcttaaa cattataata tcatctactc
     3241 tgtttaaaaa ctcaggttta aatctatgct tcatttcatt cattactaaa ttgttagtag
     3301 tctctgttat atttcctcct gcttctaata gataattact tcctatatta gaagtcatta
     3361 ttataagagt atttttaaag tctactgtct tacctttatt atcagtaagt ctaccatcat
     3421 ctaatatctg taagaacatg ttaaatacat cctcatgtgc tttttctatc tcatcaaata
     3481 gtattacaga ataaggagca cgtctaactg cttctgtaag ttgaccaccc tcttcatatc
     3541 ccacgtaccc tggaggaggt ccaactagtc ttgatacagc atgtttttcc atgtattcag
     3601 acatatctat tcttattata ttatcttcac tatcgaataa atttctagca agagtttttg
     3661 caagttcagt tttaccaaca cctgtagggc ctaagaaaat aaatgaacca attggttttc
     3721 tttcatcttt taatcctgct cttgcacgta ttactgcatt tgaaactgct gtaactgctt
     3781 catcttgtcc aataactctt ttatgaagtt catcttctag tttaagtaat ttttctcttt
     3841 ctccttcaac tagtttagtt actggtatac ctgtccactt tgatacaatg ctagatattt
     3901 cttcttcagt aacttcttct tttaaaagag aattttcaga accatcattc atattttctt
     3961 cgtactgctt tatttgttct tctaacttag gtatttcacc atatttaacc tctgctgctt
     4021 tgttgaaatc atattctctt tcatactttt cagctttacc tttagcttcg tctaattgag
     4081 cctttaagtt tttaatatct aatatttgac tcttttcttt ttcatatttt gctgtcattt
     4141 catcattttt agattttaac tctgctattt ctttttgaat atcatccaat cttgatttac
     4201 ttttatcatc attttcttta agtaatgctt ctctttcagt ttctaatgta aataactttc
     4261 ttctaacaac atctaattct gttggcaatg aatctatttc actacgaatc atagctccag
     4321 cttcatcaat taaatcaatt gctttatctg gtaaaaatct atcttgtata tatctatgag
     4381 aaagttttgc tgctgctaca atagcattat catgtattct tactccatga tgtatctcaa
     4441 atctttcttt taaacctcta agtatagata ttgtatcttc aacagtaggt tcttctgcaa
     4501 ttacaggctg aaaacgtctt tcaagagcct tatctttttc tatatacttt ctatattcat
     4561 caaaagtagt tgcaccaata cagttcaatt cacctcttgc aagcataggt ttaataagat
     4621 tacctgcatc cattgaacct tctgttttac cagctccaac aatagtatga atctcatcta
     4681 taaacagtat aatttttcca tcagaacttt gaacttcttt taaaactgcc tttaatcttt
     4741 cttcaaattc acctctatat tttgcaccag caattaaagc tcccatatca agtgagaata
     4801 caatcttatc ttttaaacct tctgggacat ctcctcttac tattctctct gctaaccctt
     4861 ctacaattgc agttttacct acacctggct caccaatcaa tacaggatta ttttttgttc
     4921 ttcttgataa aattcttata atccttctta tttcttcatc tctcccaatt acagggtcta
     4981 atttattttt cttggctaaa tctactaaat ttgttccata tctagcaagt gcatcatatg
     5041 tgccttctgg gtcttgtgtt tctactcttt gactacctct cacttgtgat aaaatattta
     5101 aaaagttatt cttatttata ttatattgtt ttaatatttt accaacagct gattttgatt
     5161 ctgtttccat cattgcaagc ataacatgct ctacacttat atatgaatct ttaaattctt
     5221 tagaaatact ctctgccttt ataagcacct catttatttt tctagtagct gatactcctt
     5281 gtgagtcagc accttctcca tatacctttg gaagcttatc aatttcaaag tttacagtat
     5341 ttctcaaaga atcaattgat atattcattt tttcgattat atttggtatc aatccatcct
     5401 cttgattaat aagtgctgag aaaagatgaa ttacatctac ttgttggtta tgatttttta
     5461 ctgcttcgtt ataagcctca tttaaacttt tttgaactct taaagtcatt ttttctacgt
     5521 ccatataaat ccctccattt taatattata tccaaataaa ttaaattctc tcatttataa
     5581 ttattgattt tctaagctct aaaagactat caagtaaatt ctcgtttttt tgtatatcga
     5641 aactcaatgt taaattaaat gttccatcta aattaaaaaa atcgaatact tcttttgatt
     5701 ttgctattaa atacaagtct tcatctataa gtcttttttc acctattaat aaatactgac
     5761 atttatctat aaaactcttt ttaaaatcaa tagtgtatag attttctaaa atattatatt
     5821 gataaatagc aatattatta aacactttta atatgtttga atttacaaaa ttgtaaattt
     5881 gattattgtt atacagtgat atttgttcgt atatatattc cctaaataaa ggatgaatgt
     5941 tttgcttaag ttttaaagta acttcagctg gtatttcaaa ataaagcttc cagtacttag
     6001 agttattttt ttctaaaata aaagctactt tgttcaaact ggtagaacta atctccttta
     6061 gtgtacaatt aaaattatag tgtctcaaac tagttttttc attctcaaat acatagtcaa
     6121 accactgtag aacatttcta tcaagtttta aactcatata aaaaccttct tccttattta
     6181 ctaaccttat taggtctttt tatctgatta tattctgcta ataaactttc tttatcagct
     6241 ctactacctt tatatgctat atgtggattt atcatatact caccattttt cactctttgt
     6301 aaaaaatcca tttttttcag ctccgtaaac aatgaagata aagaagtttt tggatattca
     6361 aattcacaag ataattcata taatgttttt ctaatcttat tatttttatc catagagttt
     6421 attaatttta atggaattaa atccctctta gtatacattc cttctactac tttaatataa
     6481 ttcttaaaac aaacattcat atacttactt ttattcataa ttaaactttt gctctctgta
     6541 tattcctgat ttaaatcctt ttttaatgtc ttatccaaat taaatatctc ttctcactct
     6601 ccttatctct aattgataat ataaatatat tacaaaaaaa tatgtatgtc aaaactgaaa
     6661 atgcaaaaag ttcaccttat aagtaaactt ttatttagct ataagctaaa ctttattatt
     6721 tttttaattt agtatttcca atttattggc aggatttttg aacaatattt ataaattttt
     6781 taaaaaaatt taaatctatt taatatggca gactttcata aaatacatta catttattaa
     6841 atgtataata ttggtaatta gttttttcta aaatcagttg acatattata tatatattta
     6901 tataatgtgt atatactata tatacaattt aaggaggtgt cttattgaat atagttatta
     6961 gtaattcgag tgggaagcca atatatgaac agattactac acaaattaaa agtatgatta
     7021 taagtggaga gttaccagaa ggctcagctc ttccaagtat gagactattg gcaaaggaac
     7081 ttagaataag tgtaattact acaaaacgag catatagcga tttagaacga gacggattta
     7141 ttgagacagt tactggtaaa ggcagttttg tatctagtaa aaacatggat tttgtcaaag
     7201 aagaacaatt tcgtttaatt gaagaatacc tacaaaaagc agttgataca gcaaaaagta
     7261 gtgatattac atatgacgaa ttaaaagata ttttattttt attatataaa ggagaatgat
     7321 aagtatggat gcaataacta taaaaaattt aaataaaact tataaagatt ttagcctaca
     7381 agatatatct ttttcagtac caaaagggag tgtaatggga tttgtaggtg aaaatggtgc
     7441 aggaaaaaca actacattaa aagcaatttt aaatttaata tcttatgata gtggaaatat
     7501 agaaatcttt ggtcttgata gtaaaaaaaa tgaaaaagaa attaaagagc aaattggggt
     7561 tgtatttgag ggaagcaact ttcatgaaaa tttaaacact gaccatgttt caaaaatcat
     7621 gagtaaaatc tataaaaatt ggaatgacac attatttaaa gactacttaa aaaaattgag
     7681 agtaccagac aataaattaa ttaaggagtt ttcaaagggg aataagatga aacttagtat
     7741 tgctgtagcc ttatctcata aaccaaaatt attaatacta gatgaggcaa ctagttcact
     7801 tgaccctatc gttcgtgaag aaatactaga tatattttta gattttatac aagatgaaga
     7861 acactctatt atcttatctt cacatattac aagtgattta gataagatag cagactatat
     7921 tacatttata cataaaggaa aaattgtatt tagtgaaaat aaggatgaat taattgatac
     7981 tatgggagtt ttaaaatgta aacctagtga ttttgataac ctttcaagag aggattattc
     8041 atattatcgt aaaagccaat ttggatatga agtacttctt aaagataagc atagatttat
     8101 ctcaagacat ccaaactgta ttgttgataa tacgtctata gaagaaatta tgttatttta
     8161 tgtgagaggt gataaataat gaaaggtcta attttaaaag atttacttaa tttgaaagga
     8221 aatataaagt ttatactttt attcatcatt atgtttggct ttatgtcttc tttaggagat
     8281 ggaaatgtta ataactttat aggtgttatt atagttttat gtactacaat gattgtttca
     8341 acattttcat atgatgattt gaataaatgg gatagttatg tacttacaat gcctattaat
     8401 cgcaatgata ttgtattaag taaatattta actatgttaa tatttagctt tataggtgtt
     8461 cttgtttcac ttattgttag tgtaactatt ggttatttta aaaatacact tatattaaat
     8521 gaaacactac ttattaatgc actaatctta tcaatttcag tatgttttgg tagccttatt
     8581 ctaccactta tttataaatt tggtacagaa agagctcgtt tattaatgat tttatgcttt
     8641 ttagttccaa cactagcttt attagttttt aaaagtattt tagaaaatat tagttcacct
     8701 atttctatag aaattatact taatacacta gtatattctc ttccatttgt tgctatttta
     8761 ctatttgtga tttcatattt tatatcatca aaaatctata gtaaaaaaga agtataaaat
     8821 atactatata tgtttctgta tttaagtatt taaataaaat actatcgagt ttattttggt
     8881 agtattttat tttttaatta ttaatctcta ctaggtattt attattttat tacttatatt
     8941 taataagact atttactact ttctaatctt atttatataa tttattcatt ttataaattg
     9001 aactactaat gatattattg aacatagcaa tagaataaca tctaaaatag tttttatgta
     9061 ttaaattaac ttattttaat attcaacaat ttaggtttac taaatttatt gatgatttct
     9121 ttttatataa cttaaactac atttttattt aaaaaaattt aatatcaaca atataaaatt
     9181 aagaataagt attattagtt tgacattagc ataaatgcct attagaataa tgttaaacta
     9241 tattctttct actgatttta ttaaaaaata ccaatataca aaagattata caaggagaaa
     9301 gttcaaatat ggaattagaa attacagaaa aaatagaaaa acaagatgaa gatactattt
     9361 ttcaaggatt attaaaatat aatcttgcta gacttgaaga taaaaaccca gtggatttag
     9421 gaatatatat tcgagataaa gcaggtaaaa ttgttgcagg ttttataggt gttactcatg
     9481 gtaattggct ttctataaaa tacttatggg ttagtgagaa gcttagatat aaaggtactg
     9541 gaagtcaact tttatataag gctgaaaaaa ttgctaaaga acgaggttgt aaatatgttt
     9601 ttttagatac attcagtttt caagcaccta aattttatga gaaatttgga taccataaag
     9661 tattttcact tgaaaattat cctctaactg gaaagagata ttattataga aaaatactat
     9721 aataataaaa atcatatttc atatttttaa attaaaactt ttgctcttcg tctacagact
     9781 ttatgttttt atatcattat taatactaaa atagtatttt actatacata caaaattatt
     9841 tgaaagttca aaaattataa gtataaaagc taatcttagc ttaaataaaa attatatttt
     9901 taggaggtaa atatgttagg aaaaaaatgt atggattatc tacaaacatt aggaaaaata
     9961 agttcaacaa caaatggtct tacaagactt atcttgactc aagaacataa aaaatctatt
    10021 gatttaatta gttcatggat ggaaggttta aatcttgata ttgaaattga tgacattgga
    10081 aacgttatag gtacatataa atcaagcttt ccaaatgctc ccacattagt agttgcatct
    10141 catcaagata gtgtaaagtg tggaggtata tttgatggta tgttaggtat aatagtgcct
    10201 ctagttggtc ttgaagaagc aaaacacaat aataggtctt atcctttcaa tattaagcta
    10261 attgctttcg ctgaagagga aggaactaga tttgaaactt ctttaatggg aagtaaagtt
    10321 tttgctggta cttttaaaga ggaacttctt aaatctgttg atgaaaatgg aattacactt
    10381 gaagaagctg taacaaagtt tggatttaat acaaaaaact taacaaattt gcatcctaga
    10441 aaagatgtag atgcatattt agaatttcat attgagcagg gtcctgtttt agaaaatgaa
    10501 tctcttcctg ctggaattgt atcaagtatt acaggattta aaagttttaa gatatcagta
    10561 aatggtaagt ctggacatgc tggtacactt ccaatgaata tgagacttga tgctggatgt
    10621 tgtgcctgtg aatgtgtatt agctatcgaa aaagtagcta aaacgactac tgatttggtt
    10681 gctactgtag gaaagatgaa tttctatcca tcttcatcaa atgtggtacc tgaaagagct
    10741 gaatttacac tcgatgtacg ctcttgttca caagaaattc ttgataattc tgtagaaaag
    10801 atatttaatg aaatatcaca tatatgtgaa aatagaaaac ttaattatac aagtgaactt
    10861 gcttttgaaa atgttcctgt accatgttct aataaaataa caaaaatcat agaaaaaagt
    10921 tttatagatt taaatcttaa tcctttttat atttatagtg gtgctggcca tgatgctcag
    10981 gaaatggata atataactga tattggtatg gtatttataa gatgcgctgg gggcgtaagt
    11041 cataatccta atgaaagtgt ctctgtagat gacttggata ctgctgtaaa aatattttta
    11101 aaaatactag ataatcttga tttaaaataa ttcttatata agaaagtata ttaaaatata
    11161 taatctaaaa aatcacttta t
//
LOCUS       DAELTA010000119        10987 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.119, whole genome shotgun sequence.
ACCESSION   DAELTA010000119 DAELTA010000000
VERSION     DAELTA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.119"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            172..1620
                     /locus_tag="KPG13_002683"
     CDS             172..1620
                     /locus_tag="KPG13_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0986385.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1646..4036
                     /locus_tag="KPG13_002684"
     CDS             1646..4036
                     /locus_tag="KPG13_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0986386.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4038..4709
                     /gene="pgmB"
                     /locus_tag="KPG13_002685"
     CDS             4038..4709
                     /gene="pgmB"
                     /locus_tag="KPG13_002685"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0986387.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            4748..6118
                     /locus_tag="KPG13_002686"
     CDS             4748..6118
                     /locus_tag="KPG13_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0986388.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            6465..7307
                     /locus_tag="KPG13_002687"
     CDS             6465..7307
                     /locus_tag="KPG13_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0986389.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7452..9380
                     /locus_tag="KPG13_002688"
     CDS             7452..9380
                     /locus_tag="KPG13_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0986390.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9441..10868
                     /gene="ascB"
                     /locus_tag="KPG13_002689"
     CDS             9441..10868
                     /gene="ascB"
                     /locus_tag="KPG13_002689"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG0986391.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
BASE COUNT         4335 a         1132 c         2038 g         3482 t
ORIGIN      
        1 tattgatatt taatatgaaa gtattgaaaa tagacaatat taattatagt gagtaaaaaa
       61 tataattgga tttttaaatt gaattattta aaaggtatag tatgaaaaaa atcttataac
      121 taaatatgag atagaaatta taaaatttta tactgtgaaa gaggtagatg catgaaatta
      181 gggaaagatt ttttatgggg aggggcaatt tcagccaatc aaagtgaagg attttattta
      241 gatggaggaa aaggtttatc tcagtttgat aaactcccat taaatgataa gagattaaaa
      301 aatgtatgtt tagatgagga taatatttta aatgaaaata atcaatacta tccaagtcat
      361 atagggatta aattttgtga aaattacaaa gaagatatag caatgttagc agaaataggt
      421 tttaaatgtt ttagattttc tatatcttgg tcaagaatat tcccaacagg agaagaaagt
      481 gaaccaaatg aaaagggatt acaactttat gataatataa taaaagaatt aaaaaaatat
      541 aatatagagc cattaataac gataagccac tttgatatac cttattctct agttgaaaaa
      601 tattctgggt gggctgatag aagagtggtt aatttatatg taaaatatgc tgaaattttg
      661 atgaaaagat ataaagatga tgtcaaatac tggattcctt ttaacgaaat gaatatgata
      721 atgcatattc catatatagg tgctgggtta acttttatag ctgatgaaaa tcaacttcag
      781 aaaaaatatc aagcagcaca ccatcaatta ttagctaatg caaaaactat agaaatagga
      841 aggtatataa ataaagaatt taagtttggg tgtatgttag ctgctggaaa aacatatcca
      901 tacacatgta aaccagaaga tgtatttgca actttagagc atgagaggca taatttgttt
      961 ttttctgatg ttcaagttag gggtgtgtat ccaagcttta tgagttatta tttaaaatca
     1021 aatcaaataa aattaataat aaaagaggaa gatttatcaa cacttaagga taatactgta
     1081 gattttgtat cttttagcta ttattctagt gcttgtagtt cagctagagc tgaaggttta
     1141 gaaaaatcaa aggcaaacgg accagaaacc ataaaaaatc catatcttcc aaaatcaaat
     1201 agtgtatggc aaaacgaccc tgtagggtta agaataacta tgaatcagtt atatgaaagg
     1261 tatcagttac ctttatttat agtagaaaat ggactgggta ctcaagatat tgtaaatgaa
     1321 gatggaaaaa taattgatga ttatcgtata gattacatga aagaacatat taaaaatatg
     1381 attcttggag taaatgaaga tggaatagag cttataggat atcttatgtg gggatgtata
     1441 gatgtaacca gtgtaagtga aggaaagatg acaaaacgat atggcataat tcatgttgat
     1501 tctgatgact atggaagagg ctcatttaaa agaactaaaa aacaaagtgc tttttggtat
     1561 aaaaaagtaa tagaaacaga tggagaaatt ttatttaatg atatggaaac tcatgcataa
     1621 ttcattttaa gaaaggagtg ttattatgag tttaatattt actactaagg accaacttgt
     1681 agagctaggg tttagtaagg atacaataaa atccaatgaa acgttgttta cactagctaa
     1741 tggacattta ggagttagag ggaatattga agagtctgat tttaataatg agtatgtaga
     1801 taatatgggt acatatgtaa atggatttta tgaagaatct ccaattacat atggagaaag
     1861 cgcttatggc tatgcaaaac aaaatcagac tatctgtaag ttacctaatg ctcatatagt
     1921 aaatttctct attgaaggag attgctttga tttatcaaaa ggtattacaa gtgaacatac
     1981 taggatacta gatttaaatg aagggattct taaacgttca tttatatggg aaaactctcg
     2041 tggtagaaaa gttaaagtat ctatagaaag attagtatca caagatatag aagaattaat
     2101 gatgatttca tatacaataa caccattgaa ttttaatgga aaaataaaat ttaaaaataa
     2161 aatggaaaat agtatggaga agtttaattg tgaaaaagta gatgacccaa gagtggcatc
     2221 tataaataag aagcaattta aaattaatgt aaaaaaatat aacaataagt attttatgaa
     2281 tttagttaca gaaagaagta gattagggtt atggtgtggg tttgattcat atattacttg
     2341 tggaaatatt acaaatgaga cctataatgt ttcagatgaa tgtattgagc gagaaataga
     2401 agtctatgcc aaaaaagatg aaaggttaaa tttagaaatt gttgtaggct atagtagagt
     2461 atattctgat ttaaatctaa atgaagaacg tttaaaatca ttagaatgtc tggagtcgtt
     2521 aaaatgttta gaatcagaag cacatttaaa atcattagaa ttggttttaa gagaagcatg
     2581 taatattgga tttgataaag tcaaaaatat tcagagaaag aaaatgaatg agttttggac
     2641 aacctctgat gtagagattg aaggcgataa aactcttcaa ctggggataa aaaccaattt
     2701 atttcatatt tatcaatcag caggaagaga tggaaagact aatatatcag caaaaggtct
     2761 tacaggtgat ggctatgagg ggcattattt ctgggataca gaaatgtata tattaccatt
     2821 ttttatatat actcaaccat ccattgctag agctttatta gattatagat actcaatctt
     2881 agatgcatca agagaaagag ctagagaact tggaataaat aaaggttgct tatattcatg
     2941 gagaactata aatggtaatg aatgcagtgc atattatcca gcaggaacag cacagtttca
     3001 tataaatgca gatatagctt atggtgttag aacgtatttt gaagcaacta atgatgaaga
     3061 atttatgatg caaaatggct tagaaattct tatagaaaca tcaagatttt gggttgaatt
     3121 tggagattat atagaatcaa aaaataataa attctgtata aatggagtaa caggaccaga
     3181 tgaatataca gcaatagtaa acaataatta ctacactaat ttaatggcta agtataattt
     3241 aatatatgca gtacagatgg tagataaatt taagcataat ttattttcaa atgaagtatt
     3301 tagaaaagtt aaattacaag aatatgaagt gaaaaactgg ataaatgccg ctcaaaatat
     3361 gtatttacca tatgatgaag agaagaaaat aagcaaacaa gacgattcat ttttagaaaa
     3421 atcagtatgg gattttaaaa atacaccaaa agaaaaatat ccattacttc tccattatca
     3481 tccattaaca atatatagat atcaagtaaa taaacaagca gatactgtac tttcagcgtt
     3541 gcttttccct gatgagttta gcttagaaca aaagaagaga gattttgaat actatgaaaa
     3601 aataactact catgattcat ctttgtcaag gtcaatattt ggaatgatgg caagtgaaat
     3661 tggaaatcat gaaaaagctt ataactattt tatggacaca gcattaatgg atattaataa
     3721 cctacaaaaa aatacaagtg atggaataca tgctgctaat atgggtggaa catggatgag
     3781 tttagtatat ggatttgcag gaatgaaaat aaaaaatgat aaattaagtt ttgaaccaag
     3841 gatacctaaa cactggaaaa atattaaatt taatatatta tttagagata atttagtttt
     3901 tgtaaatata gaagaatata aatcttatta taagttaatc aaaggtagaa atataaccat
     3961 attacatgat aataaagaaa taaaattatc aaagaacata atagaagtta ataattgttt
     4021 taaagggagt aattaatatg aaagcagtct tatttgatct tgatggagtt ataacagata
     4081 ctgctaagta tcattttgaa gcttggcatg agatagcaaa atcgctagat atagaaatag
     4141 atgaaacttt taatgaaaaa ttaaaaggca taaacaggga agaatcatta gataaaatat
     4201 tgttatctgc taataaagta gatagttata ctaaagagga aaagttaaag gtgtgtgaag
     4261 aaaaaaataa catttatata aagttaataa actcattaac atataaagat atattaccag
     4321 ggataaatga gcttataaaa gaacttaaaa aaaataatgt gaaattggga atagcatctg
     4381 ctagtaagaa tgcacctagg atattagaat ctttgggagt aaaagatgaa tttgattgta
     4441 tagtaaatcc atctttatta aaaaggggaa aaccatatcc tgatattttt attgaaggga
     4501 gcaagatgct aaatttaaaa caatatgatt gtgtaggaat agaagattct tattcgggta
     4561 ttaaagctat aaatgctgcc aatatgacat caattggtgt ggggaaaaaa gaagtactag
     4621 gaaaatgtga ccatattgta gaagatacta gcttattaaa ttacaataca ctattacaag
     4681 cttggagtaa aaagattaat ttggattaaa attatattaa aaattatttt aaaagagagg
     4741 ataaggtatg aagaataaat atgaagatgt agctcaattg ataattgatg gacttggagg
     4801 aaaagataat ataattgact taactcattg tatgacacga ttaaggttca tcttaaaaga
     4861 tgaaaagaaa gtcaatgcag ataaattaaa atctatagat aaggtagttg gagtaaactc
     4921 aacttcaact caatatcagg tcataatagg taatgaggta ggtgttgtat ttaatgcaat
     4981 aataaataag gggataaata ccacaggaga tattaaaagc gatataaaaa ataaaaataa
     5041 aaaaaatagt attttaaata atataataga tacaataact ggttgtatga cacctatgat
     5101 accagccctt acagcatctg ggatgataaa agttatatta tctctttgca ccacttttaa
     5161 ttggctatca tcaaattcaa gtacatatag agtattagat ataataggag atgtagcatt
     5221 ttactttatg cctataatac ttgcaataaa tgcagctaaa aaatttaatg ttaatacatc
     5281 aatagcagtt atagtagtag gtgtgttttt acatccaaat ttctctgcat gggtatcaag
     5341 tggggaccct atatcattta taggagtacc aattcaaggt gttatatatg cagcatctgt
     5401 tatccctgca cttttaacag tatggatgat gtcatatata gaaaatttca ttgataaatt
     5461 aacacctagc atgttaaaaa caattttaaa tccaacttta gtattattaa taagtgcgcc
     5521 aatagcatta atagtaattg gaccaatagg aaatttctta ggagagggat tagctagtat
     5581 aataaattta ctacaaggaa ggttaggatt tataatggtt tgtttattag cagctgcaat
     5641 gccatttatc gttatgacag ggatgcatca tgcattgact cctatattca tatcagcatt
     5701 tgcaactaca ggacaagaat cgctaatatt aatatctcaa gtttgtgcta atttagcgca
     5761 aggtggcgca acattagctg tagcattaag aagcaaagat agtaagatga aacagttagc
     5821 atcagctgca tggatatcag ctattatggg tataactgaa ccagctcttt atggagttac
     5881 attgaaactt aaaaaacctg ctatgttagc atctatagga gcaggaatcg cagggttatt
     5941 tgcaggaatt actcatgtga ctttatatgt tccacaaaat agtataatgg cagtacttgg
     6001 attatcaggg gaaaagggga tttcaaatat agtaaatgga cttatagtta tatttatagc
     6061 attggttgct ccatttatta tgacatatgt ttttggagtt gaaaaaaatg ttaaataaag
     6121 tacagataat gtagtagagg aagtataata ttaactattt acattaatag aatttttatt
     6181 attattgact ataacttgtt ttgtatttat attgatgcaa agaaataagt taatattaat
     6241 aagaataatt attataactg acaaaaagca acataaaaga ttgatgaagt atatggaaat
     6301 aaaaagttga caaaatatgt ctgatgtttt atcatattaa ttggaattag gtagtaattg
     6361 tttaagtaca agctaagctt attcattaat aatagcagta tcctaggaag gagtatgccc
     6421 attattatgg ataagctttt tttatgctta aaaggaggat aatcatgata attaataaga
     6481 tattaaacaa taatgttgta atcacattag atgataatga tgaagaagtt atcgttatgg
     6541 gtaaaggaat tggttatcaa aaatcaaaag gcaatctaat agataaaact aaagtaaata
     6601 aagtatttag aatatcaaat aaagaaatat caaacaaact acaagaatta ctcaataata
     6661 ttcctataga acacatgaaa ttatcaagtg aaattataga atacgcacaa ataaagttaa
     6721 acaagaagct aaatgaaagt atttacatat ccttaagtga ccatacatat tctgcaattc
     6781 aaagaatgaa agaaggaata aatgtaaaaa atgcaatcct ttgggaaatt aaaagatttt
     6841 ataaagaaga gtttgaaatt ggaatgaaag cactagatat tattgaaaac aaaacaggta
     6901 taaaattacc agaagatgag gctggattta tagcctttca tatagtgaat gctcagttaa
     6961 gtgaaggtca tactttggca tctgatatta cgaagttaat acaagaagta cttagcatag
     7021 ttaggtatca ttttaggata gaatttcatg aagaatcagt gttttattat agatttataa
     7081 tgcatttaaa gttttttgcg caaagattgc ttttagatag tgcacatgaa ggtgagacag
     7141 acaaagaatt actaagcatt attaaatcta aatataataa agagtttgaa tgtgtagtta
     7201 aaattaaaaa tttcattaaa aaacaatata actacatatt aacagatgat gagcttatat
     7261 atttaacaat acatttagct aaagtagtta aagactcaaa tatgtaagtg aaataatttt
     7321 tcagcagtat attaagaatg attgaaagga gtgatactaa caaaattata cttatggttg
     7381 gatggtaaca ttgagttggc caaaccttca aatttcaaaa aattaattta aacgaaatat
     7441 ggaggagaaa aatgggaaaa tacaaacaat tagcacaaga gatagtaaac aatgttggtg
     7501 gaaaagaaaa tattagtgga ttagtacact gtattacaag attacgtttt actttaaaag
     7561 atgaaagtat agcaaatgac aatgtattaa aaaacatgga aggtattgtt actgtaatga
     7621 aaagtggagg acaatatcaa gtagttatag gaaatcatgt tgaagctgtc tataaagatg
     7681 tagttgaaat aattggttta gatgattcaa gtacaagttc agaaactaaa aaatcaggta
     7741 atatactaga taaaggtata gatataatat caggaatttt tcagccagtg ttaggaataa
     7801 tggcagcttg tggtatgttg aaaggattta atgcattatt tgtagcaatg ggattatata
     7861 gtgatgtatc tggtggttat atggtaataa atgcagcggg agatgcatta tttacattcc
     7921 ttcctttgtt cttaggatat acttcagcta agaagtttgg attaaaacca atgttgggat
     7981 tagcactagg agctgcagta tgttatcctg ctatacaagg tagcagtata tcagcaggag
     8041 cagatgtgat gtacacttta tttaaaggaa caatgtttgc atcaccagta tatattgatt
     8101 tctttggact accaattgta tcaattgatt atactggaac agtcgtacca attatattta
     8161 tagtgtattt tgcttctagg tgtgaaaaat tatttaataa gtgtgttcca aatttagtta
     8221 agtttttctt tgtgccaatg cttacattat taataacagt gcctgttgca cttatagtta
     8281 taggccctat agcaacattt ggttctacac ttatttcaca aattgtaatt tcaataagag
     8341 attttagtcc actattggca ggtgctatag taggatttac ttggcaaatt cttgttattt
     8401 ttggtatgca ctggggattt attcctgtat atataaacaa tgttatgact ttaggatatg
     8461 ataatgttat gatgccattc tttgcctgta catttgcaag ttcggcagtt gtgttggcaa
     8521 tatttttcaa gactaaagat aaaaaactaa aagagatggc aataccaaac tttatatctg
     8581 gtatatttgg agttacagag ccagcaattt atggaatatt attgccttta aagaaaccat
     8641 ttataataag ttgtattgct agtggtattg gaggcgcttt ctatggattc tttaatctta
     8701 gaaaatttat aactggagga atgggaatat ttgaacttcc agcaatgatt gaaccagatg
     8761 ggggaatggg taacttaata gtagctcttt ctggtatagc tatatcaatg gtcgtagctt
     8821 ttgtattaac tatgatttta tataaagatg agaaagtaga agagtctaaa aaagtaagaa
     8881 ataagggtaa agaagaaata aaagaagtca agtctactaa attagaaaga gaaatagtaa
     8941 caagtcctat aaaaggtgaa gtattaaaat taagtgatat agaggatgca gcatttgcat
     9001 ctggtgtact aggtcaaggg gtggcaataa tacctagtga tggaaaagta gttgctccag
     9061 tagatggggt agttacaacc ttatttccta gtctacatgc tatagggatt ctttctgata
     9121 caggtgttga agtgcttatt catattggat taaatactat tcaattagag ggaagaggtt
     9181 ttgaggcatg tataaaacaa ggtgatagaa ttacaaaagg tcaaactata ttaaactttg
     9241 atgttgatgc aataaaagag ttggggtata gtactgtaac tccaattgtt attacaaatt
     9301 catcacaatt tttagatgta gtagaaacag aaagtaaaaa tattgaatta gaagacaatc
     9361 taattacagt cttattttag taaagattga ggtattagca taatctagtg ctttggttat
     9421 aattttagga ggctttgact atgagtttta agaatgattt tttatgggga ggagctactg
     9481 cggccaatca atgtgaaggt ggatacaatg aagacgaaag aggattggca aatgtagatg
     9541 tatgtccaac aggaaaagat agaacagcag ttattacagg gaaactaaaa atgtttgatt
     9601 ttgatgatga gcattattat ccagctaaaa ctggtattga tatgtatcat aattataaag
     9661 aagatataaa gttatttgct gaaatgggat ttaaagtata tagaatgagc attgcatgga
     9721 gtcgaatatt tccaaaaggt gatgaagaaa caccaaatga aaagggactt caattttatg
     9781 aaaatatatt taaggagtgt aaaaaatata aaatagagcc tttagtcact attacacact
     9841 ttgattgtcc aatgcattta gttaaaaagt atggtgcatg gcgtaatcga aaaatgatag
     9901 gtttctatga gaaattatgc aatgttatct ttagaagata taaaggatta gtgaagtact
     9961 ggctaacttt caatgaaata aatatgattt tacatgctcc atttatgggt gctggtattt
    10021 gctttgaaga aggtgaagat gttgaaaaga taaaatatca agctgcccat catgagcttg
    10081 tagctagtgc aattgcaact aaaatagcac atgaagttga ttcaaataac ttaattggat
    10141 gtatgtttgc agctggaagt gtatatcctt atagttgtaa tcctaatgat gtatgggaag
    10201 ctacaaagtt agatagagaa aattacttct ttgtggatgt acaatctaaa ggtaaatatc
    10261 ctaactatgc gttaaagtat atggagcaaa aaggtattac accagaaatg gaacctggtg
    10321 atattgaatt attgaataaa tatacagtag attttatttc attttcatat tacaatagcc
    10381 gttgtgttag aaccgatgaa aatgcagatg atatggctga aggaaacata tttacatctg
    10441 ctaaaaatcc atatctttca tacagtcaat ggggatggcc aattgaccca ttaggtttga
    10501 gaattacact aaatcatgta tatgatagat atgaaaagcc actatttgtt gtagaaaatg
    10561 gattaggtgc taaagatata gctgatgaaa atggttatgt agaagatgat tacagaattg
    10621 attatataag agaacatata aaagctatgc atgatgcagt ttctatagat ggggtcgatt
    10681 tattaggtta tactacatgg gcacctattg atttagtaag tgctggtaca ggtgaaatgg
    10741 aaaagcgtta tggatttata tatgtagata gagacaatag tggtaatgga agtctaagaa
    10801 gaatgaaaaa gaaaagtttt gaatggtata agaaagtaat tgcttcaaat ggtgaagatt
    10861 tatattagac aagaaatcta tgttaaataa gaaatttgta ttaaataaga agagtataaa
    10921 aattaataat aagtataatt tttaactttt ttaactatag aaaatcaata cagaattatt
    10981 ttacatt
//
LOCUS       DAELTA010000120        10952 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.120, whole genome shotgun sequence.
ACCESSION   DAELTA010000120 DAELTA010000000
VERSION     DAELTA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.120"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            347..1882
                     /locus_tag="KPG13_002690"
     CDS             347..1882
                     /locus_tag="KPG13_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0986392.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            1898..2659
                     /locus_tag="KPG13_002691"
     CDS             1898..2659
                     /locus_tag="KPG13_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986393.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            2997..3866
                     /locus_tag="KPG13_002692"
     CDS             2997..3866
                     /locus_tag="KPG13_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0986394.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            4195..4446
                     /locus_tag="KPG13_002693"
     CDS             4195..4446
                     /locus_tag="KPG13_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG0986395.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            4558..5337
                     /locus_tag="KPG13_002694"
     CDS             4558..5337
                     /locus_tag="KPG13_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0986396.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(5472..6122)
                     /locus_tag="KPG13_002695"
     CDS             complement(5472..6122)
                     /locus_tag="KPG13_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986397.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(6119..6622)
                     /locus_tag="KPG13_002696"
     CDS             complement(6119..6622)
                     /locus_tag="KPG13_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0986398.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(6671..7186)
                     /locus_tag="KPG13_002697"
     CDS             complement(6671..7186)
                     /locus_tag="KPG13_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0986399.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            7466..7993
                     /locus_tag="KPG13_002698"
     CDS             7466..7993
                     /locus_tag="KPG13_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0986400.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            7977..9380
                     /locus_tag="KPG13_002699"
     CDS             7977..9380
                     /locus_tag="KPG13_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0986401.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(9763..10074)
                     /locus_tag="KPG13_002700"
     CDS             complement(9763..10074)
                     /locus_tag="KPG13_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986402.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(10130..10723)
                     /locus_tag="KPG13_002701"
     CDS             complement(10130..10723)
                     /locus_tag="KPG13_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0986403.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         4037 a         1282 c         1783 g         3850 t
ORIGIN      
        1 aacttaacag taataaataa tgtgagaaat attcaaattt aattaaataa agtgtcagaa
       61 atttgtttaa attttagtat tttaacggtt ctgacctttt ttcttatttt tatggagaat
      121 atcgaggatg gagtctctaa tagttacttt tttgtgtcta cttgcaagtt tgattaaaaa
      181 atggctatac acacttgtga cttcagtcat gggaggttca gattataatc ttaatatata
      241 atttgtgaaa taatttgatg acgatttgct gatgtataaa caataaagat taactacatt
      301 aaaatttaaa tcttgttaag taaaacttat aaaaggagag agatggatga taaatgatat
      361 tctaacctta ttatctgaaa gaagtgattt ttttataaag ctatccatag aacatttgat
      421 gatttctatt atttctataa taattgcgat tgttttgggt ggtataattg gtattattgt
      481 aagtgaattt cataattctt ctaaacctat attgggaact atcaattttc tatacaccat
      541 tccatctatt tctatgctag gatttctgat accattttcc ggaattggaa atactactgc
      601 tgttattgca cttaccattt atgcattatt gccaatggtt agaaatacat atactggaat
      661 tactaatgtg gataaaaata ttcttgaagc agctagggga atgggaagta cagattttca
      721 gatattgtat aaaataaaac ttccgttagc cctacctgtt attatgtctg gtattagaaa
      781 tatggtgatt atgacaattg cattagctgg tatcgcttca tttataggtg ctggaggcct
      841 aggtgtagcc atatatagag gaatcacgac aaataacaca ataatgacaa ttactggaag
      901 tatacttatt gctctattag ctttaatatt tgattttatt ttagggctgg tagaaaaatt
      961 ttttcaacga aaaagaaaaa agaaaaatag gaaaaaagtt tttgccattc taggaagcct
     1021 tattcttata gtaattatct ttacgtctta tttatttaat ccttcaaaga ctcaaactat
     1081 tcatattgct actaaaccta tgactgaaca atatattctt ggtgagatgt tggatatttt
     1141 aattgaaaaa aacacaggtc taaacgtgga aattacacaa ggtgtaggag ggggaacatc
     1201 taatatccag ccagctatgg agaatggaga atttgacatt tatccagagt atacaggtac
     1261 aggctggaat atggtactta agaaaaatga attatatact gaaagtatgt ttagtaaact
     1321 acagtcggaa tacaaaacta aacttaaaat gcaatggact ggtatgtatg gtttttctaa
     1381 tagctatgca ttagcagtta gaaaagaagt tgcagataaa tacaatttaa aaaatataac
     1441 ggacttaaaa gatgtatctt ctaaattgac atttggagct gaatatgact tttatgagag
     1501 agaggatgga tatactccat tgtgtaatac atatggactg aactttaaag atacagttga
     1561 tttagatatt ggattaaaat accaagctat caatgaaggt aagattgacg taatgcctat
     1621 atttacaact gatggtcagt atagtgtttc agatgttgta gttctaaaag atgataaaaa
     1681 tttctaccca tcttatcaat gtggaaatgt tgttcgtttg gaagtattag aggaaaatcc
     1741 agaactatca aaggtattta agatggttgg agatattttg actgataaag aaatggctaa
     1801 aatgaattat gacgtagaaa acaacaattt agaacctaga gaagttgctc gtgagtttct
     1861 gaataagaaa ggtcttttat aaattggagg aaatataatg acacctatta tacaatttaa
     1921 aaatattaaa aaacaatata atgataaaac tataatagat aatcttaatt tagatataga
     1981 aaaaggagag tttttgactg taattggttc gtctggttct ggtaagacaa cactcttaaa
     2041 aatgattaat ggtctgattt tgccagatgg aggaaatatt ttgataaacc aaacagatat
     2101 caaaaacgag gacttaatta aattaagaag aagaatagga tattgtgtac agggaagtgt
     2161 tctttttcct catatgactg ttgaagaaaa tatttcatat gtaccaaatc tcctaagtaa
     2221 aaagaataaa ttagaagtaa aaagtgctgt aaacaagtgg atggaaatag tgggattgcc
     2281 aaatgatatg aaagtgcgtt acccatcaga attgtcaggt ggtcagcaac aacgtgtagg
     2341 gattgcaaga gctttagctt cttctccaga aattttatta atggatgaac cttttggtgc
     2401 agttgatgag attacacgaa agcaattaca aaaagagata aaagaaattc ataaaaaaac
     2461 aggagttacg attatcttta ttacgcatga tatttatgaa gctttgattt tgggaacaaa
     2521 gactttggta ttaaatcatg gagttgttca acagtatgat actcctgaga atattttaaa
     2581 tactcctgct aaccaatttg tagaccagtt attgaatata agaaaatcta taatagatga
     2641 tgataattta aaagaataaa tttaaattta aaatcaaatt gttgtgataa aaactattga
     2701 tttagagcta acttcaagtt gtatacttga agtggaaaag ttagattaag gtatagaagg
     2761 gcatttagca gttggctgta gacgaggtaa ctgctgaagg tgctttcaat aaatggctag
     2821 aacctgtatc tgttgaagat tataataaat tgaaataaga agctcaatat acagagtaga
     2881 taggcacttc aaatagaagt gtcttttgtg tgccataaaa taaatcaaaa tattagcaaa
     2941 gaatgtgaaa aacaaaataa aataatatgc tatctttaaa ataggaggga tgctatatgg
     3001 ataacaattt gattaccatt gaaaaaactt tagattatat agaaaaacat cttttagatg
     3061 agatgttaga tttaaatgta atttctaaag agataggcta ttcaaaatac catttacata
     3121 gaatgtttac atcagttgtc ggatttagtg tacataatta tatacaaaga cgtagactta
     3181 ctgaagcagc gagaatgcta atttttacag aaaaaccaat tattgagatt gctttaatct
     3241 cggggtatga tacacaacgc tctttttcaa gagggtttaa aaaaatgttt aaacgtagtc
     3301 ctaatttttt tagaagacat caagattttt taccactaca attaaaattt gatattcata
     3361 atagaaaaaa attacgtgga gatatggttt tagatgttaa aaaagttaat tgtgcagata
     3421 taaatattgt tggctattct ggtaaagtaa gaaaaggatt tcatattatt ggaaaatgct
     3481 ggagggattt gcataaaaat aaagagatga tagaaaataa ggtagattta ggttttttaa
     3541 ttggagttag tgattatagc aattttaaag agattgagaa tactcctaca tttacataca
     3601 ttgcaggtgc tcaagtagct acttttggca aattacctaa tggaatggaa aaattcgtat
     3661 taccgaaaag taattatatt gtttttactt ttattgggaa aaatgaggat agtatgcaaa
     3721 atgttgtaga atatatctat caagaatggt tccctaattc aagttgccag tttaatgaaa
     3781 ataatttata tgactttgta aaatatggag aaaaaactga tgagaatggt ttgagtgaaa
     3841 ttcagttttg ggttccaatc atttaaaaga aggtattatg tataaagtag atggatttat
     3901 ataggcaaag ttttacagca gtatgtattt ataaataatt tatagtttgt aggaaagaaa
     3961 taattttact ataagattat gataaaatac acaatactat gacaaaatag tgttaaataa
     4021 tagatttaat tttgcatagg aaaagttcta taatggtgtg tattcataaa taatttataa
     4081 tctgtaggaa agaaatagtt ttactataag attatgataa aatacacaat actatgataa
     4141 aatagtacta aataatagat ttaattttgc aaaggataag gtgttttaat aaaaatgaat
     4201 gtagaaaacg aattacttaa aaacctagat agattgcata caacagagtt aggcgttgta
     4261 agaattaaga aaaatctttc tttggagaca aatgatgtag tgaactggtg taaaactaaa
     4321 atagaatctc caaatgccat tattaataga aagggaaaaa actggtatat aagtgtatat
     4381 gattgtatta taacagtaaa cgctcacagt tatacaatta ttacagctca taaagaaaag
     4441 aaatgaattg caaaatacaa aggtcaaaag cattttccac tgtttgtagt acctatgaag
     4501 aagtatataa aataatatta cttttgaata ataatttaaa ttttggaggt tgaaaaaatg
     4561 aatgatactg taaaagaaaa attggcagag tcactaactt ctgatactac tgatatttta
     4621 ccttatttgc cttatttatt acaagatttt tgggaattag gaagttcacc agaacaaatg
     4681 ttacaattat ttagaaagca tatacctttt aatgaggata caaaagtttt ggatttagct
     4741 tgtggaaaag gtgctgtatc tgtgaagctt gcaaaagagt tgaatataca tatcaaaggt
     4801 attgatttga tacctgaatt tattaattat gctagaagaa aggctattga atataaggtt
     4861 gatgctttgt gtgattttga aatgaatgat gctaatagtt ctgtaaaaat tgagcatgat
     4921 tatgattgtg tgatttttgg agctgtaggg gatattttag gtaattataa agaaactata
     4981 agtaagctat tacctacaat aaaaagtaag gggttcattt taattgatga tgcatatgta
     5041 gtagataaat ctacaaatga acagcttaat tttgagaggg tgtatcccac ttacaatgag
     5101 tggtgtatat tgttcaaaga attaggtctg aagctaattg aatgtatacc atgtttagaa
     5161 cttgaaaatg atgttgaaga ggaaatgaat tggattataa ctcgtgcaaa tgagctaatt
     5221 aaaaaatatc cttctcaaaa agatatgttt gaacaatatg ttattagcca aaaaaatgaa
     5281 tatactgatt tacagcagga ccttgttgga gcaacttgga ttttacaaaa agaataagat
     5341 aaatattaga ctagataatc aatgttatag tgaattatcc catgtctagt gaatataaat
     5401 aacataaaaa accgtcaaga tgaatagttt attcgtgctt gacggttttt agtatttgtt
     5461 gaagtaaaat tttatttctt tagtatatca cttttagtca atccatttga tttagcttga
     5521 gtattccatt gatgattccc cataagttta tataggaatc tagggaagtt aggtgaaata
     5581 aaaatagtac ttttgttgga atcatctata tgtatattat cactaagtat attttttgaa
     5641 agttctttta atgcaattcc taaatctttt ttaggtcctt tacccaaagg tacggattct
     5701 agtgaaccaa acatctcccc agctccaatt ccaatacctt gaacccatgt aaagttgatt
     5761 tttttacacc aatgctctat catttgaatt gccaaatgat tatgttcacc ttcattgaat
     5821 ccacagttaa ctattgcata cactggaata ggttttttat tttgtgtttt taaaaatttt
     5881 tcacatttct ctaaaaaatg aagtaaatga gatggaatac catctacata aagaggagat
     5941 gcgattatta atctatcaca attaagtatg gcattaagag cgtcaggata cttagatgtg
     6001 ctatctactt taaatgtaaa aactttatta tcattttgaa taagttgttt taattcagat
     6061 aaaaaatact gtgaattatt tttatttact tttggactac catttatcat tactatattc
     6121 atatggattc acctcttata tcttgtatat tttcatagaa acaaacagag tgttctttta
     6181 tattgaaatt tatagcattt ccatttacta atttcgtagc agtttctttt tcagcttctg
     6241 taatacaatc tccatagaat atagctttta aatttatctg tttatcatat ctgcttttat
     6301 gacgcatcat attatctttt attacaaagt atggaagaat ataattaatg cttttatcaa
     6361 gtacgttttt gacaaaaggg ctaaaccctc cgtaacagca tctgcttatt ataataattc
     6421 catcagagtc atttataagt ttacccatgt tgctgtaata atcttttaca acacattcag
     6481 aaggagtttt aatccagcaa ccaaaacaac caatacattt gtggatttct tttgtgtttg
     6541 gaagtatttt tatattgtca tcaagtttgg gtaataactt ttctaattct ttgtcagtta
     6601 aatcatgtat taaaagcctc ataaagtcca tctcctttta ttatgtatgt ttacttctaa
     6661 aaattatatc ttatagattt ttcagtaaat ctttacacca attaataaca aaattataat
     6721 agtcaactcc ataaatcatt gttgcataca tatatgtata ttggtcaacg caatgatttt
     6781 cttctgcatc atttttttgt ttacttaatt tatttagaag taattctact tcagttatta
     6841 acatttttaa gtttgtttca attatttctt tgggtagata cttgtaaaag aatatattaa
     6901 caaggtggtc ttgctttgtt ttagaaaaat ttataggttt ctttaaccat tcaagaaata
     6961 cttctttacc agtattagtt atggaatata atttttttaa tttgctacca tctataactt
     7021 cgtgatagtg tatatatcct tttgtttcta atcttttaag tgcaggatag atacttccaa
     7081 agctggcgtc aaaaaagtac gaggtacttt ctgacattat ttgttttaag tcgtatccac
     7141 ttaattcttt ttccattaaa aatcctaaaa taatgtattc taacatatta cctcctggta
     7201 tcaattagat atatatcgat tcgatatatt tattattaca gataataaat cttacgtcaa
     7261 gatgcttttt aagataagct aaaaagaatt aggagttatt gataaatatg caaaaaaatt
     7321 aatataaaag gtaattaaat atcaatgaag atttaaaaat agttaaatca aagtaagagt
     7381 ctaatatata aaatttatag taactttatg gtttaaatat aaacttgcgt gctattgaca
     7441 aagtataaaa ttagcagtaa aataattgca tgagcaaata gattattgcg aacgcaaaaa
     7501 aataaggaca atagatatta aaggagagaa tatgagagag ctattaaaat gggtttccat
     7561 tataaataga tacaatacaa attatattga caggaaattg gcggatattg gaatcaataa
     7621 tagccaatat ttttatgtat tacacatatg taataatcca ggtattacac aggacactat
     7681 atttgagaaa attcttgtga atcctagtaa tataacaaga gctttggcaa atttagaaaa
     7741 agaaggcttt ataacaagag aaccaggtgt aaaggataaa agaacatggc atttatatcc
     7801 tacagaaaaa tctttaggat gttatgatga aatactaaaa ataacaaatg ggtatgttga
     7861 ggagttgttg agttcatttg agaaagaaga gcaagagttg tttatgtcaa tgttaaagaa
     7921 agttgcattt ttggctattg atatgaatga agaagcaaaa ttggaggcga aggaaaatgg
     7981 aagaaaaata taaggagcca ttggaatatg aaaaggtttc aaagttatta actaagtacg
     8041 caattcctag tattatagca atgttagtaa gtgctttata taacattgtt gaccaaatat
     8101 ttattgggca aggtgttggt gtattgggta atgctgctac aaatgtcact tttccactaa
     8161 caacaatatg tactgcaatt gcattattgt taggaattgg aggagcgtct aagtgttcat
     8221 tggaattagg tgctaaaaat tcagaaaaag ctacaaaagc agcaggaaat tctatatgct
     8281 tgatggctgt ttttggaata agtttattta tagtagtatc aatattttta acaccactat
     8341 taaaattctt tggctctaca tcagaaatac taccttatgc acacacatat acaaaaatta
     8401 catcaattgg attaccattt cttataatgt ccactggaat gagtaagttg atacttgcag
     8461 atgggagacc aaaagcttct atgttttgta tgttaatagg agcagttatt aatacaattt
     8521 tgaatccatt attcattttt gtttttaata tgggaattgc tgggtctgct ttagcaactg
     8581 ttattgggca acttatatct tttggaataa gtgttcatta tataaagaat tttcaacata
     8641 ttaaattaca taagaaaagt tttaaattag atagtataat ttataaacat atatttgcgc
     8701 taggttcaag ctcatgtttt aatcaattag caatgactgt aacacagata gttatgaata
     8761 atacattggc atattatggg gctaggtcag tttatggaag tgagattcca cttgcttgtg
     8821 tcggtatcat tattaaagta aatatgattt ttatatctat aattattggt atatcacaag
     8881 gtatgcaacc aattataggg tataattatg gggctgaaaa gtataatcgt gtaaaagaag
     8941 catataagtt tgcagtggga agtgcaacta ttatatctgt tattgcattc ttatgtttcc
     9001 aattcttccc aagacaaatt acgtctatat ttggtaacgg aaatgagact tattttatat
     9061 ttgcagaaca ttattttaga actttcttgt tcttaacatt tataaatggt gttcaaatag
     9121 tatcatcaac tttctttaca tcaattggga aatcagtgtt gggacttttt acttctttga
     9181 ctcgtcaaat tttattttta attccattga tagtaatttt accaatgttt atgggaattg
     9241 atggagtaat gtttgcagga ccaatagcag atgcagcagc ggcaatggta tgtatatact
     9301 ttgcagttca tgaaatgaaa tcattgacta taaagcaaaa taatttgatg agtaaccata
     9361 ataagattgc agaggtataa gacaatattt aagaagtata gaaaattaat ttaaaaaata
     9421 taataaatta atttaggaag tgtagaaaat taatctaaag aagtataata aactaattta
     9481 ggaagtatag aaaattaatc taaagaagtg taataaacta atttaggaag tatagaaaat
     9541 taatttaaag aagtataata aactaattta agaaatatag aaaattaatt taaagaaata
     9601 caatacatta atttttaagt aacattaaat taatgtaagt aaaataatgt tcatatgaat
     9661 atttaaaggt gcattttctg aagctaagta tatgttgtag agtgttatgt ctctctatcc
     9721 tgtgaagttt aatagctcaa gaaaatgcac tatttatgat gattattttg ttttatttcc
     9781 aattttggaa ataactattc taattaaatc taaaattata ccataaataa gaaaagaaag
     9841 tatagatgct atgtaccaat aaatcgaaat tgaccctgta attgttctac atgggatttg
     9901 catacctgaa tccataaaaa caaaaaaact atcaactctg gaaataatag acaaaatagg
     9961 atttaaacct attagaacaa tactttttga gtcttgacca atttgatgca tataaatctc
    10021 aaataatgca attaaaaaaa accaagatgt aaaagattta ataatttttt tcatcaaaat
    10081 acctcacata atataaattc tgtttgcact atttgcttac tacaactatt tatctccatt
    10141 cagtcacatc acttaattgt aatctaggtc ttcttgatgg ttcttcatca ggataaccta
    10201 aagatattgc tgccactaat tgactatctg tatttaacca ttcacataat tcacgatatg
    10261 caaagtatac atcacaaatc caaagactac ctagacctaa ttccacagct gtaagcgaca
    10321 tattctgaat tgcagctcca attgattgca tatttgccat ttcatagaat cgttcttcag
    10381 gggatagctt ttctaaaggt gatttaccta atatatttaa aataaaaatt gttactggag
    10441 cttgtttcat gatttcaact gtgtaatttg caccagcgat atgttgacga cttcctggaa
    10501 gaagcccatt atcattaatt tcattttgaa tacctttgct catggcttta agcatagatt
    10561 ctttttcctt ttcagtgatg acaacaaaac gccatggttg cctgttttta gatgaaggtg
    10621 catttattcc agcttcaatt attttctcta tagtttcatg tgaaatgctt tgatttttat
    10681 attttctgat acttcgtctt tttgatatag aatcacttat cattttaagt aacctccgtt
    10741 tattttttgg aaaaatctat ttaattttta ttttctattt taattatttt agcattaatc
    10801 agaaaaaaag tcattgattt tagtatacaa ttattaatga ttatattaag aattgtatca
    10861 aaaagtatgt gattatacta agggattata ttaaacatta tatttaaaat tgtatgaaag
    10921 gttgtattta atattatatt gagagatata ta
//
LOCUS       DAELTA010000121        10924 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.121, whole genome shotgun sequence.
ACCESSION   DAELTA010000121 DAELTA010000000
VERSION     DAELTA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.121"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(39..671)
                     /locus_tag="KPG13_002702"
     CDS             complement(39..671)
                     /locus_tag="KPG13_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0986404.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(743..1096)
                     /locus_tag="KPG13_002703"
     CDS             complement(743..1096)
                     /locus_tag="KPG13_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0986405.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1174..1377)
                     /locus_tag="KPG13_002704"
     CDS             complement(1174..1377)
                     /locus_tag="KPG13_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0986406.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1767..1997)
                     /locus_tag="KPG13_002705"
     CDS             complement(1767..1997)
                     /locus_tag="KPG13_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986407.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1999..2406)
                     /locus_tag="KPG13_002706"
     CDS             complement(1999..2406)
                     /locus_tag="KPG13_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0986408.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2766..3215)
                     /locus_tag="KPG13_002707"
                     /pseudo
     CDS             complement(2766..3215)
                     /locus_tag="KPG13_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3229..4155)
                     /locus_tag="KPG13_002708"
     CDS             complement(3229..4155)
                     /locus_tag="KPG13_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986409.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4280..5062)
                     /locus_tag="KPG13_002709"
     CDS             complement(4280..5062)
                     /locus_tag="KPG13_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0986410.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5218..5838)
                     /locus_tag="KPG13_002710"
     CDS             complement(5218..5838)
                     /locus_tag="KPG13_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0986411.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5926..6624)
                     /locus_tag="KPG13_002711"
     CDS             complement(5926..6624)
                     /locus_tag="KPG13_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0986412.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6725..7540
                     /locus_tag="KPG13_002712"
                     /pseudo
     CDS             6725..7540
                     /locus_tag="KPG13_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8212..8979
                     /locus_tag="KPG13_002713"
     CDS             8212..8979
                     /locus_tag="KPG13_002713"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0986413.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9307..9984)
                     /locus_tag="KPG13_002714"
     CDS             complement(9307..9984)
                     /locus_tag="KPG13_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986414.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10134..10451)
                     /locus_tag="KPG13_002715"
     CDS             complement(10134..10451)
                     /locus_tag="KPG13_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986415.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10473..10811)
                     /locus_tag="KPG13_002716"
     CDS             complement(10473..10811)
                     /locus_tag="KPG13_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986416.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3619 a         1730 c         1558 g         4017 t
ORIGIN      
        1 tattaaagta aagtttaata taaataaatt ataaaatatt aaactaattt atcaatattt
       61 cctgattgaa tatgcttaat gtttgattga atttttttat atggccaatc ccaccattta
      121 atttctaata attttgaaat tgtatcatta ttaaatctct tttttataat ttttgcaggt
      181 actcctccaa caatagaata cggtggaacg tcattagtaa ctactgctct tgttccaata
      241 attgcaccat caccaatatt tacacctgac ataattatag aatcataacc tatccacaca
      301 tcatttccaa ttataatgtt acccttattg tcccaagcgt ccgttatatg gttgatatcg
      361 agtttccatt cttcataaaa gatgggaaaa gtatatgtgg attttgattt cattgagtga
      421 tttccactag tcattagaaa ttttgcatta catgctattg agcaaaactt accaattatt
      481 aatttatcat tgtttatagg gtaatgatac aatacattgt ttttctcaaa atctcttggg
      541 tcattataaa agtcattata tattgtgtaa tctccaactt ctatattttc ccttgttatt
      601 acatttttaa gatatacagt ttgataatca ttacttcttg gatatacctt atttgaactt
      661 ggaatagtca tatttaaatc tctcctttaa tatatattat gtttttgact attccactac
      721 tacaatacat ctcttcacag aatcactcct tgttaagaat cttcataaga taatggcgtc
      781 cttttttagt gttaagtcgg atattctttc ttagtccatt ctttttagcg tataactgac
      841 aaactgtcat tttcttacca atagtgtcaa tgctatcatc aaggtcattt tgtattgctt
      901 tttccttatc ccttaatgat atatcatcac gttttccctt tgggggttta tcagtaggta
      961 caagtttcca agagtaaaca aattgtggtt taccaaatga atatacatac ttatatgcgt
     1021 atcttccatc ttttctctgg ctctctccag ttcttaaaat tctatcttta ttatctcgtc
     1081 ttttttcagt tgacatgata agaagctcct ctcaataacc gaattgagct tgatacgtca
     1141 tatgaatatt ataacatata aggctcaatt tcattagata acgtcaagtg tatcaacgat
     1201 tttctcaaat tgttttctct ttatttgaat gcgattgcta ttcataatga cccagccaga
     1261 atcaaggttt tcatcagcac gtttacgtaa ttttttctct ccaatacgaa agtattttga
     1321 cgcttcttca atcgtcaagg tatatttttc ccaaataggt acatcagtat tgttcatata
     1381 atgtatcact cctttctatg ttcgtagttc agaagtagat agatggcttt gataagctcc
     1441 attggtattt caaccaatcc cattcttatg gacagaaccg tgtcatacag gtgtatcatt
     1501 atactgtggt acaggttacg gcaaaacaac cgttccaaag gttattcaca aatcactata
     1561 tagtagctct cttgattaag gtcatggcgt atagtatttg ttggctatct gtatcacaaa
     1621 cgtatctatc tactttattc agttgtcaaa gaacagtcgg cattgatttg cctataaaaa
     1681 atcacaatag cttgtgggct tttatagaca aataattagt gattctccat gtaccaaata
     1741 gcttgataca tggagagagg gcgagattaa gacaaattaa atctcaaaat agcagtgatt
     1801 agttttgttc ctagcattcg ctttgtttct tcttctacat gaatataggg atttccgtat
     1861 tcatcaaata aagttttctg tgaaagacga atgatatagt gttcaaaatg tttaagtatt
     1921 acgtttattg catttacatc gccatgtaca gcaagtaaaa taattgtaaa aggtaatact
     1981 tctttagtag gtttcatttc aatgttcctg catgagttct ttcaactctt ttagtgcatt
     2041 tgttttgtga taatgtatgg tattttctgc taaatccatc aatgttgcta tgtcagcatt
     2101 tgtcatgttg aaaaagaatg aaagtagaat tatatcttgt tttctttttg taagttttcc
     2161 gattgcttgc gccattaata catcttcaat tcgtatgttg tagtcgtaag aaaccataca
     2221 gatgtagtta gaatcataat catctaaaat actcaattta cttaattctg caagtgacaa
     2281 agattcgaga gataccaaat aatcatttct gcgtttattt tcgtcataaa tatctctagc
     2341 atagttacgc aaaactgttt tacagaaact gtcgaattga tgttcaattg tcttttcatc
     2401 aaccataaga tttcaccctc tttccatcgc aaggagttaa ataaccttgc ttactcctat
     2461 cacggtggga gggcgttaaa tcttcgataa atttcaaaaa aaagagaaaa aaatacacac
     2521 tctgcctata gggcaaaaat gtgtattctt taaaacgtat ttaatttata ttggttgtaa
     2581 gccaccacca tattgatgta tggaaatatg cattggctag cttttcataa aaatacaaag
     2641 atgcataaga aattaataga ggggaaaaac ttattctgtt attaaaaagt aggttaattc
     2701 taataaatgc tattgcggtc gaattaaact atatacatgg caatcaatca tgtttctatt
     2761 ctttattatg aattgtgaca gtattcttca atcttaaagc cacatttttc caatacttta
     2821 cttgactctc tattgtagga atatacatta gcatgaattt tgttaaggtt gtatttttca
     2881 aaggcaattt tgcatatttc ttttactgca tttgttgcta tccctttgcc tcaatagttc
     2941 ctagcaagcc agtaacctaa ttcagcattt ttattaatag aggtatcttc ttcaaatgtt
     3001 aggctgatac ttccaatagc cctttcttga taaaaaatag catagctaag ttgtttaaca
     3061 tctgaagttg ccaaacaaaa actaacaaaa tctctagcgt tttgctccct ataagggtgg
     3121 gggaagttgt cacgaagatt ctttgcaatt tctttatcat tcgcaaactc aactaaatcg
     3181 gtaatataag cataattcca ctctataagt tccatttttc ttctcctttt atgaaacagc
     3241 tacaagaaat ctttctaagc caataccact tatcaaacag cgttctcttt tatttccaag
     3301 catttgataa gtccagtcaa caaatccacc gtcaactatt tcaacggttt catttccttg
     3361 tttgaccgat aatttgaaat tgataccttt ataataatta ttatcaacat catcattatg
     3421 aattgataac tctatattag gaaaaataag tttgatagtt tcaaccattc tgtcgaaaaa
     3481 tccgtcatta tctttgtatc cgtttctttt gcgtagaata atagagaaac gaacattttc
     3541 tatttcactt aatatatctt tataatagtt taagtgcttt tctaacaaca ttttttcaca
     3601 agtgtaagac cctgtatcta taccagatga aaccatacaa tataagccaa aatgtgaata
     3661 taaaccttta cctacaaaat tttgtgctct tattacccta gcagtagtag ccatgtggat
     3721 aggtatggta ttattttctt cttttctttt taatttatca gcaataatta ttgccaacat
     3781 atttgagggg tctgcaagtg tttctgtccc tcgtagagca cttactacgt tatattgatc
     3841 tacggagcca aaaacgctac agctacctaa tggggcagag ggagaaagca taatcgtctt
     3901 gatatccata ttgaatgctt ttctcaataa ttttgattcc aaagcatgaa tttcttctgg
     3961 atctataccg ctagggatag taaatctatt tagttggtat gactttagaa tatcagttgc
     4021 agttaaagta tttgcttgtc tatcaaacac ttctaataga agtgagttca aatctgattt
     4081 tgatagagct gataacttat caactatatt cttatcccca attttatttg taattctact
     4141 taaaatatca ctcatagtta tcctccatac tgttactttc aaaataatta tagaattgtt
     4201 acaacaacta atctaggtca tttgaccaaa aatgaaaaaa cactgaatat ttactattca
     4261 atattttttt actttgggtt tatttgatta aatcttgaaa atactgtaat gcaacccttt
     4321 cagcttcaac ctttcggaca ataggacttt gtgataattc ctctcttaac tcaagataca
     4381 ggttgatagt agcctgcaat gcattaacac agcttttata ttcatggtta gcaaccgtct
     4441 tttctaatgc ttcaatatat tggggtgcgt ccaattcaat ttttctaact ccacgaggta
     4501 atttacctaa gagaagctgt attaatgggg caagtactga ttgtcttaaa aaagataaaa
     4561 aatcaattgt ttcaaatagt tctctacgtc caatcttagt gcaagcgtaa tgaacccaaa
     4621 tccaaaatct atcttcaatc cactgtaagt ctggagttgg gaagtatgct tcttttttag
     4681 aataaatttc tgttatgcag ttgttttctt catagagaat agctgaatct tcaaccctat
     4741 ctagtaaacc atctaaggtt gtaaacttaa aatcaacatg aagaacaggg gaatcataga
     4801 gggaaattat caatcgaggt tctccaacgt gttcgccagt aaaagcagaa actaatgttc
     4861 caaactgttc cacaataagt aatctctctt cgataacttc ttcataggat ttatcatctg
     4921 ctacaatcag aaaatccaaa tcagaatatt catctaacga ttttgtgata taagaactgc
     4981 taattgcaag tcctattatc ctactatctc tttttgaaaa ttctaatacg ttttgtatca
     5041 ttttctgttg tgttttagtc ataatataac acctctcact gtcttttatt agctatcaat
     5101 ccaacataat tatgcatagg agtattatat aatgaagaaa taaaaaaaaa taggtcactt
     5161 gacccgaaat aaaaaagcat tgaataatta ttattcaata cttttttgaa attatactta
     5221 tgttgtagga agattcttta atacctctcg ctgattaatt acactaaatc ttttgttttc
     5281 ataagcaatt agatgttctt gtacacattc atttatagca cgattaatgc ttctaattgg
     5341 agcgtttact tcgctagcaa gctgtttttt ggtaagcaga tttaattcac ttctataata
     5401 gtgtaaggat atacacctca gaagtcgttc tttaacactt aaagatgata attcttcaat
     5461 taattcagca tttacaacta atttactaga tatttctcga atcagaaatt ttgtagcttc
     5521 aaaatctccc cttaaccagt ttaaaaaatc atctctatga agtttatata cattacaatc
     5581 tgtgaaagcg acaatttcaa cgggtttttt cccatcatta aattgttcca tttctccaaa
     5641 aaaactacca cttctataaa gatgaatcgt tgagatgttt cctttcacat ttaagatgaa
     5701 tgcttctgca ataccgtcaa tcataagaat tacatattca ttttcttgct cagcaaaaag
     5761 aatagtctct ttctttttat acgttttttt aacaagcttc tttttaatat cttcattcat
     5821 aaaatcaatt aaatgcatat tcatccctcc ttaccaaatc tttgattaaa ttatatcatc
     5881 tttcaattag ttaggtaaga tagtaaaaat atatttacat agaaactatt ccattattat
     5941 tttccagttg tcatttttct gcaaaaactt atcactgtca cgactggaga taccagtaag
     6001 gaatgttagg atatcaatcg ctaaactttc agaatccttt ttacggctca aaatgacgta
     6061 agggtcaagc aatcctttgt tttcatcatt actggttaaa ttgacaatgt ttatctcatg
     6121 ggctatttct ggtagcgttt ctttccattt gccgcgttct gctttagggt caactatgac
     6181 cgcctgtccg ccgaatagta ccgcatagta aacaagcata ttgttactga ataatttacc
     6241 accacccaat gaaccgataa acgcagacgc taacgcattg gtaatagaac cctttacccc
     6301 ttgactagca agggcaggtt ttagatagac attctttcca gtgtccagat tgtagccaag
     6361 atagatatcc tcatgttcgc caagggtttg tgtcgctcca aaaccaagtc cagccaaaaa
     6421 atccgaagtg acatattgaa tataatcatt gatgtaacgt ttgctgacag gaataaattc
     6481 gccatgcaat cccaacatat ccccaaaggg acgcacgagc ttgattgata agtcgtcgta
     6541 aaagtctttg acttcatcac aacgcctttt tagctcgtca agattctttg tagatacacg
     6601 gacaacatag ctaagtttat acatagattc tttacagtta aaagtctgtt tttaaaaaaa
     6661 tgtataaatt ataaatcaaa atatagatac tatcaatgac ccactaggtg caaaaagcat
     6721 tgatatggtt aaattatcag acgctaaaag ctgggctatc cgaatgagtg aagatgggtg
     6781 tgcttataaa tccattgaca attataagcg ctcactgaaa gcgtcgtttt tcatggcaat
     6841 tcaagacgac tgtattagaa agaatccatt taatttttag ttaagtgatg ttttggaaga
     6901 tacaacagaa ccaaaaatcg ctctaacacc agaacaggaa atgggcttat tatcctttat
     6961 ggggagcgat aagacttatt gtaaatatta tgatgatgtt gttatcctct tagaaacagg
     7021 actacgcatt tctgaattat gcggtttgac tttagaattg gatatgaaaa ataaagccat
     7081 tatggtagac catcaattgt tgagtgatac taagattggc tattatgtag aagttccaaa
     7141 gaccacacag ggtaagcgag aaattccaat gactgaaaga gcttatcaat catttgaacg
     7201 tatcgtggaa cggcgtaaga acgctaaagc agaaccaatc attgttgatg gttaccagaa
     7261 cttcctattt cttaaacaag atggattacc aaaacaagca actcattatc aaagcatgtt
     7321 aagagcgtta gtcaagaagt ataataagac ccatgaggac aagctaccaa agattacgcc
     7381 acataccttt aggcatacac tctgtacaaa tatggctaac aaaggaatga gtcctaatat
     7441 attgcaatat attatggggc ataaaaacat tactatgacg cttggctatt atgcacatgg
     7501 ttcatttagt tcttcgagag ctgaaatgga acggttataa tcctagtagt atgactagag
     7561 tttactactt atttactact tttgatggca ttcttatacg gttaaatgcc aacttatgca
     7621 aagtatcttc caaaaagaaa atgccgatac tccttgtaga tacaggatat atcgacattt
     7681 accaacttat gaaaagataa tcaaatttaa tataagtttt tcaaataaaa agataccaaa
     7741 ttctttttcg agaatctata taacattact taagcttggt tggggtataa acaattgagt
     7801 agctactttt gtatggtgtt caactttagt caaaactctt aagtaatgca aatgatttag
     7861 attcattaat attcaatctc ttcataagta gtgaaattcg agttatttct gcataattat
     7921 actatgattt atagattaat tctattagca tccagaataa tattgtttat atgagtaaaa
     7981 tttaaagaat attgtgaaaa ttataggatg ttaaaataaa tttaggtaaa aaagaagtgt
     8041 ttgtatatta tagagtacct tttctaaaat atgtgggatt gttttaagaa ttagaataaa
     8101 aattattttt aaattagtat ttttataatg tatagtattt ttaatgtata attaaattgt
     8161 gcagaaaata gaatttaaag tcctatagaa tttctgatta tggaggagaa tatgaaaaga
     8221 aaagttcaag taaaaaatat tactattgga gaaggtagac ctaaaatttg tgtaccaatt
     8281 attgggaaaa ataaaaaaga tattataaaa gaggctaaag agttaaaaga tgcatgtttg
     8341 gatattatag agtggcgagt tgattttttt gagaatgtag aaaatattaa agaagttaag
     8401 gaagttctct atgaattaag aagttatata catgatatcc ctttattatt cacatttaga
     8461 agtgtagtag aagggggaga aaaattaata tcaagagatt attatactac tttgaataaa
     8521 gaaatttcta atacaggttt agtagattta atagatgtag agttatttat gggagatgag
     8581 gtcatagatg aagtagttaa ttttgctcat aaaaaagaag tgaaagtaat tatatctaat
     8641 catgatttta ataaaactcc aaaaaaagaa gagattgtat ctcgtttatg tagaatgcag
     8701 gaattgggcg cagatttacc caaaatagct gttatgccgc aaaatgaaaa agatgtgtta
     8761 gtgctacttg aagctacaaa tgaaatgttt aaaatttatg cagatagacc aataataaca
     8821 atgtcaatgt caggaatggg tgttataagt agattgtgtg gtgaaatatt tggttctgca
     8881 ttgacttttg gagctgcaaa aagtgtttca gcaccagggc aaatatcatt taaagagtta
     8941 aattcagtac ttaatctatt acataagagt attaattaaa ttaattatat agaaatatta
     9001 gacggtattt ttgaagatat aaattaaata taaaaaactt ttcagatagt aaaatttaaa
     9061 ttgggcaatt atatctgaaa agcaactatt tagtgtgtat tttgaaagtg atatagaaaa
     9121 tcttataaat caactatggc atatttaaaa tatgccatta aaacctacta gacattagat
     9181 tatagatggt atactagcat ttctattatt tatagatagt aagttttcat aataactgtt
     9241 ttatctaaag tagaagatat atttgtatta tgtatatttg tcattatttt tttgtaatta
     9301 tgtatattaa tggttatata tgtaatataa tattatcact ctattttttg tatattgtac
     9361 attacaacat ttatatctat catcatcaat tacagtagaa tgtatgggat acttattttc
     9421 acttaaccaa tatttttgca tattctttgt ccaagacttt ggaaacttca atactttctt
     9481 gtttattata acatcctcat cttcaaaaat attaacttct ctttctattt ttatttttct
     9541 gttgtatatc ttattgatag attctttgtc gagttttatg tctctataca tatcagggca
     9601 atagcaacaa acataatttc ttttttttcg acgtttaaat ttcacaactt tctctatgct
     9661 aatttctaat ttttttgtac catcttttat aaatttaata tcttctatgt tctcgcttga
     9721 aaaaatttct gtcaatctta aagtatctac agtttctcta attatttgct ttttataaga
     9781 agagaatcca tatccaaaag aaaggtttaa tttattaggg atgtagccat ttttatattt
     9841 aaacatctta attttttcca attcagcatt atctttaaat ttcaactttt catacctaaa
     9901 agttactttg ctaaaaagag tatattctac atcaatatat tcttttgcaa tcttaaattt
     9961 atcgctacct tcaatcacaa tcatagccta tctccaatcg ttttatatta aaataaggcc
    10021 atagaacatg gtctaatagt actcattagc actataagac aatgttaaca caaaaagaca
    10081 aaaaaaggtt atttatttca aataaccttt ttttttatat aaaatataaa gaattatatt
    10141 ttattctttt ttatgatgtc tataatctgt gatatcagat ttagttctct tgaatttaag
    10201 tcttttaact cttcatttat atcattttct aaatatgttt ttccagtgga agtaatatct
    10261 tttataagaa catctgcaga aatatttaat atatttacaa attttatgaa tgttttaaga
    10321 cttggattct ttttaccact ttcgatttga gttatatacc aaggcgatac tccaaccata
    10381 tttgcaaatt cttttgaagt taaatttgct tttttccgtt cttctttaag tcttttacct
    10441 atataattca ttattgttac tatttccctc acttatattt ctttgtattt aatgatgcac
    10501 tttattaact ctagcattgc aatttgttct ttttcagtta atttattaat catttcataa
    10561 cattctaaag gtacagagtt atcaacgcct tctgttgtaa taatatttgc aaataacaat
    10621 tcaagtggta tgtctaaagc agtagcaatt ttcattataa tattgataga tgtatttgtc
    10681 tcagctcttt caatctgacc aatatatgtt ggatgtaaac ctgccttttc tgctagaaac
    10741 tcttgactat atcccaattt tgttctatag acttttattc tatctcctat tatttttcct
    10801 atatttgcca attattatcc ctccacttta gattatattc atagtttaag ttgttttata
    10861 tagactattt atactttttt agtctaaata tagactatat atgtttttat actgaaataa
    10921 gtgc
//
LOCUS       DAELTA010000122        10833 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.122, whole genome shotgun sequence.
ACCESSION   DAELTA010000122 DAELTA010000000
VERSION     DAELTA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.122"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..483
                     /locus_tag="KPG13_002717"
     CDS             <1..483
                     /locus_tag="KPG13_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986417.1"
                     /translation="REPGNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMD
                     FVSSSYNIGLYIKLCWIVDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSM
                     KVIQLLKSNDRTQLFQTIMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK
                     "
     gene            503..1516
                     /locus_tag="KPG13_002718"
     CDS             503..1516
                     /locus_tag="KPG13_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG0986418.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            1532..2329
                     /gene="cas5"
                     /locus_tag="KPG13_002719"
     CDS             1532..2329
                     /gene="cas5"
                     /locus_tag="KPG13_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG0986419.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            2356..4764
                     /locus_tag="KPG13_002720"
     CDS             2356..4764
                     /locus_tag="KPG13_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG0986420.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            5205..6152
                     /gene="prmA"
                     /locus_tag="KPG13_002721"
     CDS             5205..6152
                     /gene="prmA"
                     /locus_tag="KPG13_002721"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG0986421.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            6171..6929
                     /locus_tag="KPG13_002722"
     CDS             6171..6929
                     /locus_tag="KPG13_002722"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG0986422.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            6931..8229
                     /gene="mtaB"
                     /locus_tag="KPG13_002723"
     CDS             6931..8229
                     /gene="mtaB"
                     /locus_tag="KPG13_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG0986423.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            8272..8622
                     /locus_tag="KPG13_002724"
     CDS             8272..8622
                     /locus_tag="KPG13_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG0986424.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            8785..8964
                     /locus_tag="KPG13_002725"
     CDS             8785..8964
                     /locus_tag="KPG13_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG0986425.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            8994..9437
                     /locus_tag="KPG13_002726"
     CDS             8994..9437
                     /locus_tag="KPG13_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG0986426.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            9548..10045
                     /locus_tag="KPG13_002727"
     CDS             9548..10045
                     /locus_tag="KPG13_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG0986427.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            10238..10801
                     /gene="raiA"
                     /locus_tag="KPG13_002728"
     CDS             10238..10801
                     /gene="raiA"
                     /locus_tag="KPG13_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG0986428.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
BASE COUNT         4558 a          981 c         2019 g         3275 t
ORIGIN      
        1 agagagccag gtaatactag aaatttgggt aaaagaatga ttgataatgg gaatttatca
       61 aatttaaaag aattattact tgataatata agtgttttaa agcaatcaac tggaacttct
      121 cgagaaatgg attttgtatc tagctcatat aatatcggtt tatatataaa attgtgttgg
      181 attgtagatg gaggggagga gtataaaatg aaaaactata tggaaagtaa tttaatatat
      241 tcaagagtag gtaaagactt atttaataaa atgacagatg aatccagaag aaaaaatttt
      301 tctatgaaag tgattcaact acttaagtct aatgatagaa ctcagctatt tcaaactata
      361 atgcatgtgt tagtttctaa tggaatctta ataggagaag ggtttgtaga aggtctaatg
      421 cagagttctg aattagaatt aaatacgaat gtaggtctat ttattcaaga aatcatgaaa
      481 taaaaaaggg ggaaaattta aaatgaatga aaaaagagta aaaggagtaa cattaactat
      541 actgacagaa agcccagttc cactttcata tgaccaaggg tatggaaatt atacaccaat
      601 aaaaaaagaa caatatagag aaaaaataca tgcaaaaact agtatagcca caataacata
      661 tgatttaaga agaatgctcc accaagaata tggttggaat ttaagtaata ttgtttttgg
      721 aaaaaaagga aatatatatc catctattaa gaaaaatgta ggatgtgtag atgaaaatgg
      781 attagagact gatgtatttg gttatttaat accacttaaa gataaaggtt ctataagtaa
      841 agcttctcca cttagaataa taccttttag gtcattaaat ccatataaag gttcaacaca
      901 gctaataaca aatagaggct ttttaagtag tgaatttggt agaaaatatt atgatgagaa
      961 ggaagaaaat gaagtaccta gagatgaaaa ctttccaaca tcacaagcat tagcaataga
     1021 agagacttta tcagattact acgtttatac aataacattg gagcttgata gaataggagt
     1081 agtagaagta gaagatggaa agttactttt accagaagaa agaaaattta tgagtaagga
     1141 gttaagagaa aaagcagtaa aagatatatt agatgcaatt actgtgttta caagaaacat
     1201 aaaacattca tcagtactat taaaaccact tgcagtaata ggtggagctt ttgataaagt
     1261 tgtaccattt ttttgggatg atgtagatta taatgctgat agtggtgaga taaatttaga
     1321 gggagtcata gaaactatag aaagttatag ccttaaagaa agtaacacaa tacttgctat
     1381 caatgataga ttaaaaattt ctaataaaaa ggaattaaat aagtttaatt taagtaaata
     1441 ccctgtaaaa gagattaaaa atctagcaga tagattggaa attggagaag ataatatgtg
     1501 gtatttaaaa gagtaggagg ttttttgaat tatgaaagta ttacaatgta aacttaagca
     1561 accaactgct cactatagag acccaaaagt atttcaaaat gaatatataa gtactttaaa
     1621 tttaccatct aagaccacaa taatgggaat gataacgtat ttatgtgata gaagattaaa
     1681 ctctgatata gatataggaa taattggaac acatcatcat agagaactag aattttcaag
     1741 aggagaaaac attgattttt ggaatgaata tataaaaatg ggaaaaggta aagataaaga
     1801 aaagtttttg cttaaaggaa actactatga ttactataaa gagcataaag ctcaaaatag
     1861 catattaaac tatgaagttc ttaaagaagt agaactgact atatttattt cttgcaagga
     1921 tgatgaagaa cttgaattta ttagaaaaaa gcttgaaagt ccatgtaaat atgcaaattt
     1981 aggaagaaaa gaagattttg taataccaag tgaaaaaggg tgttttgtga aagaagttaa
     2041 gttaaaagag gttataccta tgaatactcg tgatgctata aaagaaaata taaaattaaa
     2101 gaatacatat gtaagagttg acttaagaga taaagagaat gtcgagacta taataaatca
     2161 aggtgtattg attgcattgc ctcataaata taaagattta gaagcaaata gagatgatag
     2221 agtttatgag ttttgccatt atatatatgt tgataatgat ggtatatatc ctaaaaatat
     2281 taaggtaaat gtatgtattg atacaaaaga agtttttaca tggctttaaa ttattttgaa
     2341 agataggtgt ttatcatgaa aaatatcatg caaaaagaaa tattagctaa gtcatacaat
     2401 ttaagaacta atcaacaaga acaaacatta gttgaacata ttaaggattt atttgaagta
     2461 ttagaatcta tattagaatt aaatctatat tcagataaag atgtagagat actaaaaata
     2521 tgttgtgctt tgcacgattt aggaaaaatt aattcaatta tgcaacaaaa aatggagatt
     2581 aataataaaa tatcgtatag ttgctcagaa gaagagagga aaaagttaga gtctgacaaa
     2641 aaatctttaa aaaagatagc aagacataat atattttctg gagcattttt aaaagatata
     2701 ttagaaaaaa tgaacttgtc agaagaagat aagttatata tatataaaag tataatgtta
     2761 catcatggaa actatgaaga ttatatgagg ttaagtatta gtaaagttca agaagaaatt
     2821 tatgagtata ttgaaaaagg cattttagaa aaagaagagt ttaatctgaa agatatagaa
     2881 agttatataa atagcatttt aaatgtagac tttaaatttg gtgaagatgt attggattat
     2941 gattacatag ataaattaag tgaaagtatg tttatagaca gtgattataa caatggtcaa
     3001 atagatgatt cagttttaaa ttatagaaag tttaagtata ttttatataa agggatgctg
     3061 aacttaatag accatagtgc atcaagtaga cagaaaggta taaaatttta taacgatttt
     3121 actgatgaag aaattgataa tatgatttta aatgagatat ataagagtca gggtaatttg
     3181 gaaaaaaata atatagagtt caatactata caaaaaaggt tgagagaact ttctggaaga
     3241 aatgttctga ctgttgcatt tacaggttca ggaaaaacag ctgcagatta tagaaagact
     3301 tttaaaagaa aattcttttt agtgccaaat aaaatatcag cagaaagttt ttatagaaaa
     3361 aatatttttc aaaataaaaa tgatttagat acaagaagta gtaatgatta cattggatta
     3421 cttcatggag atataaactt atattcagag aatgaagata atggagaaca tgattttgta
     3481 ttgacattaa gggatataga tttgagtata aacttttgca aaccttgtgt aattgcaaca
     3541 gttgatcagt tattacttag tatgtttaaa tttccgggat atgaaaaaat attcgcagcc
     3601 gtaaaaaatg catctataac tgttgatgaa gtacatttat tggaaccaaa gatgtttttg
     3661 attttgatat attttatgca gtttgcatgc aagtacttag atgttgattt tcacttaatg
     3721 acagctacac ttccaaaatc atataaagag caaatgataa acaagggaat tatttttcaa
     3781 gaagaaagta atgaaaatgt tactgataaa ggagaaatag tttttattga gagcaacaag
     3841 gaagaagaga tttgtgaagg taaagatgta aaggtttctt ttataaagga aaaagaaatc
     3901 aagtcaatag ttgaagatgc tttagaaaat aaacaaaaaa ttcttataat aaagaatact
     3961 attgattcag tcaatagaac atatgagttt ttaaaagaaa atttatctga taagtatggt
     4021 ggtgtagata tagatgtttt acatagtaga tttaagttca aagataaaaa agagaaatat
     4081 agtaaaatat taaatggaga gggtgatatt tggatatcaa ctcaatcagt agaaatatcc
     4141 ttggatttgg atttcaatat aataataagt gatttagcaa ctatggatag cttaatacaa
     4201 agaatgggta gatgcaatag aaataataag tatgaatatg gaaactttta tattctacct
     4261 agtgaagata aaatttatga caacaaactt aagagcacaa ctaaaaacat cttaaaggac
     4321 atattaaaaa ctaactctat ttttacaatg ggaattagaa aaactatttt ggatgattac
     4381 tatgataata gtgtagtcaa aaaatatttt gaagagaatt ttataagttg tgataaagaa
     4441 attaagaata tttatggtgt aaataaagaa atttttgatg gattggattt aatttttaat
     4501 tttgaacctt acaaaaatat tgttgatagt aagaaggaag cagctaaaat ttttagagat
     4561 gttgatgtta gttacaaaat tattctggaa gaagattttt ataaggaaga tagagagtta
     4621 caacaagatt cgattcaagt atcagggttt atatttaata gattatatta ttatgggtta
     4681 ataagcaaag tagaaggata catggtacta aaatctagta gtaagtttga gtataatagt
     4741 actgtaggct taattttgaa gtgagcatat gtttaaatat aatatacctt tagacattga
     4801 aaatacaaaa agtactactg taagctcact gtaaaaacta ttttttaaaa ggaaaaataa
     4861 gttgtgagat agagtgttta caagtgtata tagataataa atttgaggtt taagattaac
     4921 taagtgggtt tcaggtacat cgcgaaaata attataagtt ggagattttg tatgtttaag
     4981 attaactaag tggatttcaa atattgatat agtagatata atagaagttt tattattagg
     5041 tttaaaatta actaatcgga tttttaaata ttctgactat tatataagga tattattaga
     5101 ttgaatttaa aaccaataat ataaaacaac aaaatattat atttatgata taataaaagt
     5161 tatggaaaaa caggaatatt actatagaga agagaggtaa acttatgaat aattggatag
     5221 aagtaactat aaaaacaaca acagaagcag ttgaacctat aacgaatata ctatatgaac
     5281 aaggagctgg aggagctgta atagaagacc caaaagactt tttattccaa aagaaaaatg
     5341 aactagactg ggattatgtt gaagaagaag tatttaaaaa gaatgaagaa gatgatgttc
     5401 ttataaaaac ttatgtttct gaagaaaaaa atgtgatgga atttgtcgaa ataataaagc
     5461 aaaaagtttt aggattaaaa gattttggta tagatatagg tgaaggaagt gtatcattgg
     5521 accaagtaaa tgaagctgat tgggcaaatg catggaaagc atactacaaa ccaacaaaag
     5581 taggacaaag ggtagtagta aagcctacat gggaagatta tgctatgcaa gagggggatt
     5641 taatcataga gcttgaccct ggaatggcat ttggtacagg tacacatgag actactagta
     5701 tgtgcattag agaattagaa aagtatgtga ataaagattc taaagtattt gatattggat
     5761 gtggtagtgg aatccttgca atagcagcag ctaaattagg agcaaaagaa gttgtagctg
     5821 tagatttaga tgaagtggct gtaaaagttg caaaagaaaa tgttcttgaa aataaagttg
     5881 aaaaaagtgt atctgtaatg catggtaatt taactgatgt aataaaagat aaagcggatg
     5941 taatagttgc aaatataata gctgatataa taaagatact tgcaaaagat gttcaaaact
     6001 ttatgaaaga agatgctata ttcatatctt caggtataat tttggataaa gtagaagaag
     6061 ttaaagaaag cttaattgaa aatggatttg aaatagttga agttcaaaaa ttaggtgaat
     6121 ggtcagcaat agtttctaag cttaaaaaat aagggatatg gtgacgtata atggatagat
     6181 tttttgttga aaaaaataat ataaatctac aagataaaac ttgcactata gaaggtgaag
     6241 atgtaaaaca tatttcaaaa gttttaagat gcaaattagg tgagaaactt gaaatatgtg
     6301 acaagaataa taatgagtat atttgtgaga taatgaatat agataagagt atagttaatt
     6361 tagaaatttt agaaaaagtt gatataaata gagaatcaga acttaaagta aggctatacc
     6421 agggacttcc aaaagctcca aaaatggaaa taatacttca aaaattaact gaagtgggtg
     6481 tagaagaaat aatattagtt caaactaaga gaagtgttgt taaagttgat gacaaaaaag
     6541 aagataaaaa atttgaacgt tgggaaagaa taatttatga agcagcaaag caaagcaaaa
     6601 gaggtaagat accaaaatta agaggtgttt taagttttaa agaagcttta gaagatatga
     6661 aaaaaaatag tgtaaatatt tgcccttatg aaaatgaaag aacagtttca ataaaacatg
     6721 cactaaaaaa atgtgattct aatatagata gtgttggaat ttttataggt ccagaaggtg
     6781 gattttctga agaagaaata gaacaaatac aaaaaaataa ttgtaatgtt gtatcattgg
     6841 ggcctagaat attaagaact gagacagctt ctgttgtagc atcaacaata gctttgtatg
     6901 aactaagtga tttaggagga gaaaaataaa ttgaaaaaag tagcttttta tacacttggg
     6961 tgtaaagtta atcaatatga aacagaagct atgctagaac tatttgagaa agatggttat
     7021 gaacaagtca atagtgaaga gtatgctgat gtttatgtta taaatacatg tacagttact
     7081 catatgagtg atagaaaatc tcgtcaatat ataagacgag ttaaaaagaa gaatccagat
     7141 gcaataatag ccgttgtagg atgttattct caggtatctc cggaagaaat tttggatata
     7201 gaagaagtaa atttagtcat gggtacaaat gatagaagaa agattgttga agaaattaaa
     7261 aagataaatt caagtaagaa agttagtaca gttgatgaca taatgaaagt taaagctttt
     7321 gaagaaatag aaatcagcca aactaatgga aagactagag catttatgaa aatacaagat
     7381 ggatgtgaca gattttgtac ttattgtatc attccttatg cccgaggacg cgttagaagt
     7441 agagatattg atagtatagt cgatgaagtt aaaaaattgg ctaataatgg ctataaagag
     7501 gttgtattaa caggaataca tgttgcctct tatggaaaag atttaaagga tagggatatt
     7561 aaacttcttg atgtaataaa acaaataaac cagatagaaa aaatagaaag aattaggctt
     7621 agttctgttg aaccaatttt atttacagat gaatttgtaa atgaagtttt aaagatggat
     7681 aaagtttgtc ctcattacca tttatcactt caaagtggat gtgatgaaac tctaaaaaga
     7741 atgaatagac gatacacaac tttagagtac aaaactattg tagatagatt gagaagtaaa
     7801 atgccagatg tggcaataac tacagatgta atagtaggtt ttcctggaga gacaaatgaa
     7861 gagtttaaaa aaacgtacga gtttttaaaa gaaatagagc tttcacaaat gcatattttt
     7921 aaatattcac caagaaaagg aactccagct gcaactatgg aaaatcaagt tgaccctcaa
     7981 atgaaacact ttagaagtga acaactttta aatttaagta aagtaaattt taataaattt
     8041 gcaactaaat ttatcggccg tgagttggat gttttatttg aacaaaatat agaaggcaat
     8101 aaatatgagg gattaacttc aaattatata agagttgttg ttgaaagtga caaaaatata
     8161 caaggtcaga tattaaaagt gaaaataaat gatgtaaaag atgaatatgt agaaggaatt
     8221 ttgctataac atgtagaaat tataatattt ataagatagg aggtggaaag aatggattgt
     8281 attttttgta aaatagcgaa cggagaaata ccttcaacta aagtttatga agatgataga
     8341 gtcttagctt ttaatgactt gaacccagtt gctccatatc atatattagt tgtacctaaa
     8401 aaacattatg atagtttaat tgatattcct gataaagaga tggacattgt gtcacatatt
     8461 catgttgtta ttaataaaat agctaaagag aaaggttttg accaaactgg attcagagtc
     8521 ataaataatt gtggaagtga tggagggcaa gaagttaaac atttgcacta tcatatttta
     8581 gctggtaaaa agcttcctaa ttacgaagcg ggacaaaact aaactaaatt attgaaaaat
     8641 tgagttcctt cttgataaaa atacttgata aaagtaaaaa tttagtttat aataaacaaa
     8701 gtgttaggtt gtaatgcata ttatgcaaaa agaacatagt tacagtccca gcataattgc
     8761 gctattggag ggagggaaaa agaaatgtca gaagtaagag ttagagagaa tgaaacatta
     8821 gacagcgctt taagaagatt caagcgtcaa tgtgcaatgt ctggcatcat gtcagaagtg
     8881 agaaaaagag agcactatga taagccaagc gttaagcgta agaaaaaagc tgaagcagca
     8941 agaagaaaaa acgctaagaa atagtagtaa ataagaataa agaggtgaag gggatgtccc
     9001 ttaagcaaaa gttacaagag gatttgaaat cttcgatgaa aaacaaagat acagtgagaa
     9061 aatctgtagt aactttaata agagcttcta taaagcagta tgaggttgat aatagggtcg
     9121 agcttgatga agatggaatt atcgatgtaa tagcaaaaca attaaagcaa cgtagagatg
     9181 cattagttga atttgaaaaa gctggaagag aagacttaat aaaagaaaca gaaggtgaaa
     9241 ttgaagtttt aaaagagtac ctacctcaac aattaagcga agaagagtta gaagaaattg
     9301 taaaatctac tatatccgaa gtaggggcaa cttctatgaa ggatatggga aaaataatgt
     9361 cagttatcca accaaaagtt aaaggaagag ctgacggaaa acttataaat aagttggtaa
     9421 aacaaaactt acaatagatt gatatagcct agagagaaat ctctaggctt tttatagttt
     9481 ttgggaataa cgagtcatat ttgagcatat ctataatcaa agaaaatttt gaaagaaggg
     9541 gaaaaatttg attatatcag taaaagagat taggaatttt ttgataaaca tatttatacc
     9601 actaataata ggttacttaa gtagcatctt aacaatgatt atcagtggaa cagatatttc
     9661 aacctattat ttacaactca caaagccaag ctttgcacca ccagctttta tctttcctat
     9721 agtttggact atattgtaca tattgatggg tatatcttca tacctaatat taagaaaggg
     9781 ttacaactta cctaaagtaa gagatgcaat tttttattat ggattacaac ttgtattaaa
     9841 ctttatttgg agtatactat tttttggatt tggacttaga tttacagctt tgatagatat
     9901 aataataatg attttggttt ctataataat gatttcgaag ttttctaaaa tagataagag
     9961 atctggatac ataaatttga tttatttaat gtggcttgtt tatgcaggat ttttgaacta
    10021 ttttatatgg tttattaata aatagaatgt aaagattttg taaattaccc tgtactttta
    10081 ataatatcat aacaaacgaa aaataaatag actattaaaa ataatcattt tcatatacaa
    10141 taatattaat ttaaatacaa taaatttata aaaattgtat ttaaagatta acatattaaa
    10201 gtattagaat tggaaaggct tgaagggaga ggtttttatg caaataatag tatctgggag
    10261 acaaatgaaa ttaactgacg gaataaaagg atacgtagat ggaaaattaa gtaggttaga
    10321 aaaatacctt gatcctgaat cagaagtgaa agtaacagta agtgcgaaaa aggatagaca
    10381 aaaagtggag gttacaataa ttcctattaa cggtcaaata ataagagcag aagatgtaga
    10441 agatgactta tatgcagcaa ttgatattgt ttgtgacaaa ttaagtagac aagttgtcaa
    10501 atataaaact aaagttaaag acaaagttca aaataataag agcataagat ttgaaaatct
    10561 tgactttata gataatagca gtgaatttga tgattatgat tatgatgatg aagaagatga
    10621 aaatatagta atagaaagaa gaaagaaatt taatgtaaaa cctatgagtt ctgaggaggc
    10681 gatacttcaa atggaactag taggacacaa tttctatatg tttagaaacc aagataattt
    10741 tgaaataaac atagtgtata agcgtaaagc tggtggatat ggtttaatag aacaagatta
    10801 ataaaaattg tatacgttta gattttatat aag
//
LOCUS       DAELTA010000123        10825 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.123, whole genome shotgun sequence.
ACCESSION   DAELTA010000123 DAELTA010000000
VERSION     DAELTA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.123"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            66..257
                     /locus_tag="KPG13_002729"
     CDS             66..257
                     /locus_tag="KPG13_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG0986429.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            541..3042
                     /locus_tag="KPG13_002730"
     CDS             541..3042
                     /locus_tag="KPG13_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG0986430.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            3202..3870
                     /locus_tag="KPG13_002731"
     CDS             3202..3870
                     /locus_tag="KPG13_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986431.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3854..5131
                     /locus_tag="KPG13_002732"
     CDS             3854..5131
                     /locus_tag="KPG13_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0986432.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5532..5753
                     /locus_tag="KPG13_002733"
     CDS             5532..5753
                     /locus_tag="KPG13_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986433.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(6042..6806)
                     /locus_tag="KPG13_002734"
     CDS             complement(6042..6806)
                     /locus_tag="KPG13_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0986434.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            7080..7754
                     /gene="fsa"
                     /locus_tag="KPG13_002735"
     CDS             7080..7754
                     /gene="fsa"
                     /locus_tag="KPG13_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0986435.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7876..9270)
                     /locus_tag="KPG13_002736"
     CDS             complement(7876..9270)
                     /locus_tag="KPG13_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG0986436.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(9348..10694)
                     /locus_tag="KPG13_002737"
     CDS             complement(9348..10694)
                     /locus_tag="KPG13_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0986437.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         4415 a         1264 c         1676 g         3470 t
ORIGIN      
        1 cttttataaa gtttatattt gggtataaaa gtattaatta gacattaatt aggaggaact
       61 taaatatgat aagagtatgt ccaaactgtt caaatgtgga tgtaaataag ctaaaaactt
      121 tggttggaga agaaaatgta aagactggtt gtataggtca gtgtagagca tttaaaaaag
      181 aagctgttgg aataatagac aatgagcttg aaataaagga aacagaagca gagttttttg
      241 aagcatgtaa agaataaatt ttaaataaaa tataataaat agagcatcta aattgtataa
      301 gcattaaaca atttagatgc tctattttta tatatacatt taattatgag tatacttttc
      361 tattaaatac aaataaatat aactaataca tttatttatt aaaaaaatat tgatttaata
      421 tacctatagg gggtataata aaaatataga tttctaaata agtaatttat caatattaca
      481 tatcaaaata aaacgttaca aattagctga aaatatattg aaagaaagga agattttatt
      541 atgagttcaa ataaagtggt agaaagttat aaaattacag gtatgacatg tgcagcttgt
      601 gcaaaggcag tagagagagt aactaagaag atggatggag tatatgacca aagtgtaaat
      661 atagctactg aaaaacttaa aatagaatat gataattcta aggttaattt tgatgatata
      721 aagcaggttg tagaaaaagc aggatatgga attataaagg aagaaagtaa taaaaaaatt
      781 gatatgaaaa ttgatggaat gacatgtgca gcttgtgcaa aagctgtaga gagagtggta
      841 aaaaaattgg atggagtaga aagtattagt gtgaatattg caacagataa agctaatata
      901 gactatgacc catcaaaagt aaaattatct caaataaaag cagctattga aaaagctgga
      961 tataaaccaa tagaagaggt aaaaaataag gttgatgtgg atgaagataa attgagaaaa
     1021 gaaagagaaa tgaagtcttt atttgttaaa tttatagttg caatagtatt tgcagtacct
     1081 ctgttttata tagcaatggg acctatgata ataaagccta tagggccatg gcctttgcca
     1141 gaaattataa atcctatgac aaatactttt aattatgcat taattcagtt aattttagta
     1201 ataccagtaa tgatagctgg ttataaattt tatataaatg ggtttaaatc acttttttct
     1261 ttaagcccta atatggattc tttagttgca ataggaacat tagccgcatt tttatatagt
     1321 ctatatacga cactacaaat agccaatgga caaattcaag gtatgcacca tcatcagttg
     1381 tattatgaaa gtgcaggaat aataatagca cttatcttac ttggtaaata tttagaatca
     1441 aaatcaaaag gaaaaacatc agaagcaatt aaaaagctta tggggctaca acctaaaact
     1501 gcaatagtgt tagttgatgg taaagaagtt gaaactccta tagaagaagt agagatagga
     1561 gatatactat tagtaaaacc aggaactaaa atacctgtgg atggagttgt aatagaagga
     1621 tatacatcag ttgatgaatc aatgcttaca ggtgaaagta taccagtaga aaagaatgtt
     1681 ggaagtaagg tgacaggagc tagtattaat aaaaatggtg ttattaagtt taaggctgaa
     1741 aaaattggtg gagatactgc tcttgcccaa ataataaagt tagttgaaga tgcacaaggt
     1801 acaaaagcac ctattgctaa acttgccgat actgtatctg gatattttgt accaatagta
     1861 attgcaatag ctgtagttgc atcattattg tggtttttaa taggaggaaa agatatagta
     1921 tttgtactta ctatatttat atcagtactt gtaattgctt gtccttgtgc attaggatta
     1981 gcaacaccaa ctgctataat ggttgggact ggaaagggcg cagaaaatgg aatcttgata
     2041 aaaggtggag aggcattaga atctgcacat aaagtaaata cagtgatatt tgataaaaca
     2101 ggtactataa cagaaggaaa gccaaaagta acagatatag tattaaataa taatgtaaaa
     2161 gaagaatatc ttatcaagat tgcttcaagt gcagaaaaag gttcagaaca tcctcttgga
     2221 gaagcaatag ttaagtatgg agaagagaag aatataaaat ttgaaaaagt tgataatttt
     2281 aaagccatac caggtgctgg tatacaagtt actattaatg atgaaagtat attacttgga
     2341 aatagaaagc ttatgaatga caataatata aagcttgggg atttagaaga aaaatcaaat
     2401 atattggcaa gccaaggtaa aacacctatg tatatagctg tagatggaaa tttatcaggt
     2461 ataatagcag ttgcagatgt agtcaaagaa agtagtaaaa aagcaataga aatacttcat
     2521 gatatgggaa taaaagttgc aatggtaact ggagataatg cgaaaactgc aaatgctata
     2581 gcaaatcaag tcggtataga tatggttttg gcagaggttt taccagaaga taagtcaaaa
     2641 gaagttgaaa aactacaaaa tcaaggaaag tttgttgcaa tggtagggga tggaataaat
     2701 gatgccccag ctcttgcaaa agcagatata gggatagcaa taggaagtgg aacggatgta
     2761 gccattgaat ctgcggatat agtacttatg aagagtgatt taattgatgt tccaacagca
     2821 attaaactta gtcatgaaac tataaaaaat ataaaacaaa acctattttg ggcatttgga
     2881 tataatacaa ttggtatacc tgtagcagca ggaatattat atgtatttgg aggaccttta
     2941 ttaaacccaa tgattgcagc agcagcaatg agcttaagtt cagtttcagt cgtatcaaat
     3001 gcacttagac ttaaaaactt caaggcatat aagagagatt agattaataa attaaataaa
     3061 atgtatccaa aagagctatc tttaaattaa aaggattaat ttacaaatta agatagctct
     3121 tttttcatgt aaaattcaca acaataagat aaaatataag taatatacga aattttaaat
     3181 aaaatagaaa ggattgatag aatgaattcg tcaatacttg taattgaaga tgattcaaat
     3241 atacaagagt taatatcaga atttttaagt gcagaaggtt atcaagttga tacagcaaat
     3301 gatgggttgg aaggtataca aaagtttaag caaggaagtt atgatttagt aatattagat
     3361 ataatgatgc caaacttaga tggttatgga gtttgtaaaa tgataagaaa gtcatcaagc
     3421 gttccaatta tatttttaac tgctttaaat gatgaaggag accaacttaa agggtttgac
     3481 ttagaatgtg atgattatat aacaaaacca ttttcattta atcttcttat aaaaagagta
     3541 gaagcaattt taaggcgaag taacaagact ataaatgaca aatttatagt ttttgaaaaa
     3601 ttaaaattag atttaaacac atatattgca gagatagatg gagaacctat agaacttaca
     3661 ctaaaagagt ttaatatatt aaaagctttg atagaaaagt atccacaagt tataactagg
     3721 gaaggtcttt tggatagtat atggggttat gactattatg gagatactag gattgttgat
     3781 gcacatataa aaaatataag aaaaaaaata tcattaccat atattaaaac tgtaaaagga
     3841 ataggatata cacttgagaa ggatatttga taagtgggaa aagttaagta taaagtataa
     3901 gttattttca ataacgacat cattattaat agcattagct ttaataatat atctaatttt
     3961 atatttttta ttgccatcat attatcatga gtataaaatt gagtctcttc aagaaagttt
     4021 gaaatcttta gtagatagtt caattcattt cgatacatat actttagaag aaagacttta
     4081 ttatatggct aaagaccaaa atttggctat tttattaaaa gataaccaag gtaaaattgt
     4141 atatggtaag aatgaagtag ttattttaag atatagtaag tacatgatta atagtcttga
     4201 ggatgagtat agaacttcta tacctatata tactaaagat gcaaaagatg gtccatatac
     4261 tttagaactc gttatgccat tacaaccaat tgacgaagca aacgaagtta taagaaaact
     4321 aatgccatac atcatatcaa tagcaatatt gatagcgatt attggagcat atatatattc
     4381 aattgtaata acaaagcctc ttataaacat aatagagagt gagagagaac aggaatatag
     4441 aaggaaggat tttgttgcta caatatcaca tgaactaaaa actccaataa ccataataag
     4501 tggtcaaatt gaaggtatga tttatagtgt aggtaagtat aaggataggg atacttatct
     4561 gaaaaaatcg tatgagtgta cacaagaatt aaaagattta gtaaatgaaa tgatagaggt
     4621 ttcaaaatca gaaatacttg aaaaagattt gaaattggta tcaataaata taagtgaatt
     4681 attaaataga cttgtaaaga gacaagtatt tttaatagaa gaaaaacata tgaaaactat
     4741 tttaaaaata gaagaaaatt tggaaattaa agcagaccaa gagagaataa ctaaggcaat
     4801 caataatata ataaataatg ccataaaata ttctcctgaa gaatcagagc ttataattag
     4861 actctatgat aaaaataaac gaataagtaa aaagacttct aatcaaagag tggttttaga
     4921 aattgaaaat actggtgtaa ctatcgagaa acgttattta gaagaaatat ttaatccatt
     4981 ttatagaata gaaaaatctc gttcgagaaa aactggagga agtggacttg gactttatat
     5041 agtcagccaa atttttaaaa gtcatgggtt tgattatagc ataaaaaaca aagaaaactc
     5101 tgtagtattc acagtagaat ttaaaaatta attacttatt ataaagaata tataactttc
     5161 aataatagtt cattttaaaa tcacaaaaaa ttcataatga aactgtatta tataaatata
     5221 aataagctta aaaccataaa atacaccaac tataatgatg agatttcctt acatcctcca
     5281 tataagtcat cattatagta acccaacaat taagttacta tgaatttaaa aaataacacc
     5341 gagcatataa tctatgctta gtgttatttt tattttgaaa taaaaaatat ttaatttatt
     5401 gaaatgtata gatttagata gtttaaacta tatttaagac ttaaaaatta taattgatat
     5461 tacaaataaa tatatgtact ttgggaaaaa taaatatagg aagtattata tttacagagg
     5521 aggtagtttt gatggataga ttaaaaagag atatgaaaaa tatgaaagaa gatttaaaag
     5581 aaggtgttaa aaagacagct tatgttatga aaaatacagc tgaagatttg aaagatgatg
     5641 cacaaggtgc tatggtaaac ttagaaatga aaaaagatga gatgatggaa agatatgaac
     5701 aaaagaaatt ttctaaagaa ttagagaagc aaatgaagaa tgaaatgaaa taagctttta
     5761 tttatgaata acaaattatt gtattcatat aaaaagtagc cagtaatatt aaatagttta
     5821 tttaaggaaa tagactttct aaaaataact gtattagatt tagaaagtct atttcaaatt
     5881 agttttataa atagacataa ggataaagta gatgttatag aactagattt taaaaaatag
     5941 atactaagga taaaataaat attatagaaa tatgttttta agaatattta tcaaataaaa
     6001 atttatattt ataagaaatc ctataaaaca tataattaaa cttataaaac atttacatta
     6061 aattctgata aaatatctac tatatcagga gtaggcgttt tttctgtaat tatagaacta
     6121 atatcttcta aatgagcaaa cttgaaattt ccatcaaatt taaacttttt atcttccata
     6181 actataaata cttttttact tgaatttatt atagccttct tcgtaagacc atcttcaatt
     6241 tcaaaagttg taagtgaaga gttgaataca tctactccac aacttcccat aaaagtctta
     6301 tcaaacttat atttcgatat aaactctata gttggagtac cgacaaaacc gtctaaactg
     6361 agatttaata aacctccagt ggaaattata tttaaattat ttggttttga agtaactaca
     6421 tttagtatat ccagcatatt tgtaacaagt gttacttttt tattactttc agctaacaac
     6481 ttcgcaagca agatgttaat tgtagagata tctaaaaata tagtttctct atcttcaatt
     6541 aaattaaatg ctttttctgc tattattttc tttgttggaa tattaatttc ttttctgtct
     6601 tttgtatcct gattctctag tgtttctcta gctagtacag ctcctccata aattcttctt
     6661 attgaagaaa tcttttctat attttttaaa tcttttctta tacaatcttc tgtaacatta
     6721 aatttagaac tgagctcttt gacagtaact ctaccttctt tttttaggat atttaatata
     6781 gcttggattc tttcttctgc aaacattttc tacctctttt cgcaatatag tattatagta
     6841 aatattataa gataaaatac aatatatcaa ctattttaac atgaaataca aaattaattt
     6901 aatataaaaa gtatgttttt tctaactata tatttagaat ttagtaaata aaaaaattat
     6961 aaaactaaga aggaaaagta aaacaataat aaaaaataaa aaacaataag caaaatgttg
     7021 aaaataatac caaatcaata tataataaga ataaagaaag attaaagggg gatattttaa
     7081 tgcaactaat cttagataca ggtaatgtgg aggagattaa ggagttatgt acttgtttac
     7141 caatagatgg tgtaactaca aatccaagta ttgtttcaaa agaaaaaaag aattttaagc
     7201 aacttataaa tgaaattggt gagattatag gtgaggatat gcctatacat gctcaagtgt
     7261 taagtacaaa gtatgaagaa atattagaag aagcattata tataagtagt ttgagaaaaa
     7321 atatatatgt aaaaatacct gtaacacaag atggtttaag agctataaaa gaccttcaca
     7381 gaaaaggtgt aaaaataact gctactgcaa tattcactgc tcatcaaggt tttttagctg
     7441 caaaggctgg agcaaattat attgcacctt atgtaaatag gcttgataat atatctggtg
     7501 atggagtagc tatggtttca gaacttataa aaataataga tacttataaa atggatacaa
     7561 aagtacttgc agccagtttt aaaaatgcac aacaagttat agaattaatg caacatgggg
     7621 tgcatagtgt tacagtacca tatgatattt gtaaatctat gatgaatcat ccattaactg
     7681 attggagtgt aggtaagttt atagaggatt gggagaatac ttttggaaaa ggaagtaaaa
     7741 caaataatat ttagtattat ggtaaaagag tagttttgtc caatttcaca agaacttata
     7801 taaaaagatg aaaatatgaa ttttcttttt aataatttcg ttattatctt aagttaattc
     7861 atattttcat cttttttaga tttctagtcc tataaatttc caccaaggga atgttataca
     7921 tattattata aagtaggtta aaggaatcaa agttaaaccg tatttgaaaa taaccttgct
     7981 attatacaga ttttcaccat gaccaaccat tattgtagca tgatgaaatg gtaatatgta
     8041 ctgtatgctt actataatgt agacaaatag acataaagta acactatttt cctttagtat
     8101 atgtaaactt ccaagtaacg gtattactac agatgatgta gtgacagcac ttccaagaca
     8161 tatattcaag attattgtga gagttatcaa aaacaatatt gaaataattt ctgaagagtt
     8221 tggtataaac tttatcaaat aatttcccat tatatctaca actccagagt actttaacac
     8281 tcctccaatt gaaaatgctg ctataaaaaa aataaggacc ttccaattta tttcttttaa
     8341 agtactaaat ccaactattt ttcttagtaa taaaagaagt acacaaattg acattataat
     8401 taatgtgttt atgttatgta tgaactgagt taaaaacata aaaaacatta ttcctaggca
     8461 taaaaatacg tgtttttgct ctttgtttag caaattaaat tcttttttta aattagaata
     8521 tgaaaaatga agtttattac cctcaagatt ttcattagaa ataaatttta ttgaatgcaa
     8581 ttctttttta aagacaaaga taaataaaaa ataagttata aaacaagtaa ttatagttgg
     8641 aacagacata taaaaagccc attgacccca atttatatta caatttccaa gttctagtac
     8701 aacataattt agaagggtgt ctccatttac aaaaaacatt gatgtaaagg tagatgctgt
     8761 aaatatacta aataaaagta tagattttga atcttcagtt atctgttgtt cttttaaaaa
     8821 ctccttgtaa aaagcagata caagaataac ccttggaaat ggttgtggga taaaaaatat
     8881 taaaagtata cctaaaatat acgataaaag aatcattttt ttaggggtat ttacagtatt
     8941 taaaattaac ttctcagaaa taatttttgc gacacctgtt tttgtaactg cattagtaat
     9001 tatgtatgca agaattatta tgtaaaaatt ttctgtaaat aaaaactgta aaataacctt
     9061 tattggtgca atgttaaata taaacgctag aattacaaaa aacaatgcaa caaaagtttt
     9121 ttcaactgag tctacagccc atatcaagat agttaaaaaa gataagcata tagaaaattt
     9181 tatgttgaaa ctgtaattaa gagagaagta gagaattatg gtagctataa cagtcatata
     9241 aaattgtttt ggcttaagaa taatacccat ttttatattc acctccataa tttattatat
     9301 cataatggag gtgaatacaa tgaatgtgaa tacattttat aaaaagatta ttcattgatt
     9361 ctatgattta caatagcttt agttttccaa tgtcctttct tatagtagat aactgtaaca
     9421 ataagaccta aagtccaacc aaaaggaata cccatccaaa ttccgtctgt tccaaaaaat
     9481 ttagaaagca tataagaagc tggaagtctt ccaagccata aagatactat tgagcttata
     9541 agtgcaaaca tggaatcacc agcacctttc attgcacttg aaagtacaaa agaagttcca
     9601 ataaataaga agaatggtcc tattataaat aagtaagaag agccaatttt aatgacatct
     9661 ttatcagtat tgaatagagc aattagattt tccctaaata gcaaaattaa agcaataact
     9721 gtaactgcta aaactaaggt catttttata gattctcgaa tgcctttttt aactctttca
     9781 ttttctccag cacctatgtt ttgagcaaca aaagtagaaa cagcagaacc aagattcata
     9841 ataggtaagc ttataaaatt ttctatccta agaccagcac caaaagctgc catagcagat
     9901 gtaccataac tattaactaa aaattgaaga gccatatttc caattgaaaa caacatttgt
     9961 tgaactccac ttggaagtcc taatctaagt ccatctttaa atattttgtt atcatataca
    10021 attcctttga ttttaaattt aagcacttca tgttttttat ttaaataaat tatacttatt
    10081 ataaatgata ctgcctgagc cattatagtg gcaagtgcaa cgccctcaac acccattctt
    10141 agcactacaa taaaagttaa gtctaaaact atatttagaa ttgtggctat tatcagaaaa
    10201 taaagtggag ttactgaatc tccaagtcct ctaagtattg cacttactga attatatcca
    10261 gcagaaaata atattccact aaatattata gtaagataca attttgattg agcaaaaacg
    10321 gattctggtg tattcataag cttaagcatt gggccaccaa atactattcc aagaatactt
    10381 attaatatag aaccaataaa taaaagtata tatgtagtat caatagtagc ttttaccttt
    10441 tttaaatctc ctgagccata aaattgtgac actaaaatag aaaatccaag gctaacaccc
    10501 ataagcgctg ccaccaataa aaacattata gggttagctc cagagactgc cgccattgct
    10561 tcttttccga taaatctacc aactattata ctgtcagctg tattgtatag ttgttgaaat
    10621 aaactaccaa taagcatagg catggcaaaa aagaatatgg actttccctc atgacctgta
    10681 gttaaatctt tcaccttatt cctccaaata aaaaaattat taatttatta tagtataatc
    10741 ttaacataaa ctttatatcc aaactataaa taaattgtat caaaatatca tatatttttt
    10801 taaaatgtaa agaatataat tataa
//
LOCUS       DAELTA010000124        10769 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.124, whole genome shotgun sequence.
ACCESSION   DAELTA010000124 DAELTA010000000
VERSION     DAELTA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.124"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            17..1339
                     /gene="dnaA"
                     /locus_tag="KPG13_002738"
     CDS             17..1339
                     /gene="dnaA"
                     /locus_tag="KPG13_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG0986438.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1581..2687
                     /locus_tag="KPG13_002739"
     CDS             1581..2687
                     /locus_tag="KPG13_002739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG0986439.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            2824..3030
                     /gene="yaaA"
                     /locus_tag="KPG13_002740"
     CDS             2824..3030
                     /gene="yaaA"
                     /locus_tag="KPG13_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG0986440.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            3048..4163
                     /gene="recF"
                     /locus_tag="KPG13_002741"
     CDS             3048..4163
                     /gene="recF"
                     /locus_tag="KPG13_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG0986441.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4164..6065
                     /gene="gyrB"
                     /locus_tag="KPG13_002742"
     CDS             4164..6065
                     /gene="gyrB"
                     /locus_tag="KPG13_002742"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG0986442.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6085..8511
                     /gene="gyrA"
                     /locus_tag="KPG13_002743"
     CDS             6085..8511
                     /gene="gyrA"
                     /locus_tag="KPG13_002743"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG0986443.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8580..8876
                     /locus_tag="KPG13_002744"
     CDS             8580..8876
                     /locus_tag="KPG13_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG0986444.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            8876..9136
                     /locus_tag="KPG13_002745"
     CDS             8876..9136
                     /locus_tag="KPG13_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG0986445.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9148..9465
                     /locus_tag="KPG13_002746"
     CDS             9148..9465
                     /locus_tag="KPG13_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG0986446.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            9490..9876
                     /locus_tag="KPG13_002747"
     CDS             9490..9876
                     /locus_tag="KPG13_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0986447.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            9876..10649
                     /locus_tag="KPG13_002748"
     CDS             9876..10649
                     /locus_tag="KPG13_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG0986448.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4405 a         1115 c         1991 g         3258 t
ORIGIN      
        1 ctgttcggag gtttatatta tggatatagt ttctttatgg gacaaaaccc tacaattaat
       61 aaaaggtgac ttaacttcag taagttttaa tacctttttt aaaaacatcg taccattaaa
      121 aatacatctt aatgatttaa tcttactggc tcctagtgat tttaataaag atatcttaga
      181 gaatagatat ttacatttaa ttgaagatgc tattagtcaa ctgtcattaa aaaaatataa
      241 tattaaattt gtattgagcg aaaaagaggt tgctgattta aattctgata gcacagattt
      301 aaattataga gttctttacc ccaatctaaa tccaaaatac acatttgata cattcgttat
      361 tggtaatagt aatagatttg cacatgctgc atgcgttgca gttgcagaat ctccagctaa
      421 agcttataac ccactttttt tgtatggagg agttggatta ggtaagactc acctaatgca
      481 tgctataggt catcatattg ttagtcaaaa aaaagactca aaagtagttt acgtttcttc
      541 agaaaaattt actaatgaac ttataaactc tataaaagac gataaaaacg aagaatttag
      601 gaataaatat agaaacgtag atgtattact aatcgacgat attcaattta tagcaggaaa
      661 agaaagaact caagaagagt ttttccatac attcaatact cttcatgaag caaacaaaca
      721 aataattata tctagtgata gaccgccaaa agacatacct acactagaag ataggctaag
      781 atcgagattt gaaatgggac ttataactga tattcaagca cctgactttg agacaagaat
      841 tgctatactt agaaaaaagg ctcaactaga acgtatagat gtgccaaatg aggttatgtc
      901 atatatagca aaaaatatta agtcaaacat aagggaacta gaaggtgcat taactagagt
      961 ggttgcttat tcttctctta gcaatagggt aatatctttt gatcttgcta cagaagcttt
     1021 aaaagatatt ataacaactt ctaaaaatga agaaataaat gttctcagaa taaaagaaaa
     1081 agtatcttct gtattcaatt taaagatgga agattttaat tctaaaaaaa gaacacgttc
     1141 aatagcttat ccaagacaga tagctatgta tttaacgcgc gagcttactg atttatccct
     1201 tcctaaaata ggtgaagagt ttgggggtag agaccataca acagtaattc atgctcatga
     1261 caaagtatct aaggatatag aagaaagtga agaaattaag actaaaatag acaaaattat
     1321 atcagatttg aagggataat ttatacacaa ttgtatgttt atatctcttg aataacctgt
     1381 taatactttt tttgaacaaa aattattaac aaattatcaa gtctaaaata acaaagatat
     1441 taacatctta tacacatgtt aatatctttg tatttataat gttatacata cttattaaca
     1501 ttatcaacaa cacttactac tattactact atgattttaa cttattttta tatataaaaa
     1561 gccagaggag gttatccaca ttgaaaataa tttgtaatca aaaaatccta gcaaatagaa
     1621 ttggaattgc tcaaaaagct attaatggaa aaacaacaat agaattatta aaaggtatac
     1681 ttatatctac agaagaaggt caattaaaac ttactggata tgatgctgaa ataggaatag
     1741 aaacttatgt acaagcagaa atcatagaaa aaggtgatgt tgttgtagat gcaaggctat
     1801 ttggagatat aattagaaaa ttaccagatt cctttgttga gatagaaact gattcagaaa
     1861 ataatattta cataaattgt gtaaattcaa gatttaaaat taaaggttat gcagcaaaag
     1921 aatttcctaa attaccagaa ttaaatgaag aagatttata tagcatacct caagaaatcc
     1981 taaaaaatat gattaaacaa actgtatttg ctatctcaca ggaccaaaca aaacctgttt
     2041 taatgggaga acttttagaa attgtagata gaaacttaaa tttagtagca attgatggat
     2101 atagattagc tgtaaaaagt tgttcggttg atagtttaac tgaaaatata aaagtcataa
     2161 ttcctggaaa gacacttata gatgtaaata gtttgctttc tggagaggat aatgttaaag
     2221 ttggatttaa tgaaaaaaat gctattttta taattaatga tacgaaaatt attacaagat
     2281 tgcttgaagg agattttatt gattataaaa aattattgcc aagagaacat aacagcagag
     2341 ttaaattaaa tactaaggaa cttttaaata gtatagagag agcgtcttta ttgtctcaat
     2401 cagaaaaaaa taatcttata aagttatcta taagagataa agtaatggca attacttcta
     2461 atacagaaaa aggaaatgtg tatgaagaag ttgaaataga tttagatgga gattacctag
     2521 atatagcttt caattctaga tattttatcg aaggtttgaa aaatatagat aatgaggaaa
     2581 tatttataga atttactaca aatgtaaatc cttgtataat taaaccaaca gatgatgtta
     2641 attatattta cttactgctt ccagtaagaa tatcatcaaa tatatagcag acatgaagga
     2701 gttgtctttt gtagaaaatt tttctaatct tagacaactc cttttataag catatttatg
     2761 gtataatata cttttagtgt atcaatagga atttgtttaa aatagtgaca aggaggaact
     2821 attatgactg aaataactat agaatcagaa tatataaagt tggatcaatt tcttaaatta
     2881 gcagagattg catcaacagg aggtcatgca aaatttttaa ttcaagaagg tttagtgaca
     2941 gtaaatgatg aaatagaatt aagaagagga aaaaagataa aatcaggaga tatagttgaa
     3001 atagaaggaa ctaaaataaa agtattgtaa aaaaaggaga actttttgtg aaacttaaaa
     3061 gtttgcaact tgttaatttt agaaattata aaaagttgca tctggaattt aatgggaaag
     3121 taaatctact tgttggtaaa aatggtcaag gaaagacaaa tatagtagaa tctatttata
     3181 tgctttcttt tggaaaatca tttaggacta acaaagataa agaaatggtt agatttaata
     3241 gtgaaaactt atatattgga ggaagttttt caaaatataa taaatatagt ctaatagagc
     3301 taattattgg aaaagataaa aaagggataa gaataaataa agttccttta caaaagatac
     3361 aagaactact tggcaactta aatgttgtta tattttctcc tgaggattta aggttggtta
     3421 aagagggtcc taaagaaaga agggcattta tagacaaaga gattagtcag attataccta
     3481 aatattataa atatcttacc aattataata aaacactttc tcaaaggagt agggtgctta
     3541 aaaatataca tgttgatgaa gctttattag atgtatatga tgatacgtta gcaaagtatg
     3601 gaagttatat ttatattttg cgtagagact ttataaaaaa gatagctaat atatctgaaa
     3661 atatgcatat gaatttgact aatggagttg aaagattatc aattcgatat aaaaatcaaa
     3721 taaatataac tgatgaagat actatagata cagtatataa taaattttta gccaagttgt
     3781 catcgaatag acctaatgat atagaatcta agactaccag atatggtata cacaaagatg
     3841 acttaaatat attcataaat gatttagatg caagactttt tggttcacaa ggtcaacaaa
     3901 gaactgcttc aatatcatta aagttatcag agatagaatt aataaaaaat gaagttgaag
     3961 aatatccagt attaatttta gatgatgttt ttagtgagtt agatgaagca agacaaaaac
     4021 ttttagtaaa taatttaagc aatgttcaaa tgtttataac aagtgctgaa gtttcacata
     4081 aaaaaatatt tgatgaaaaa aatgttacaa tatttaatat tgaaaatggc gatgttatta
     4141 gcatagagaa tggaggaaat taaatgaaac aagaatacgg tgcaagtcag atacaagtat
     4201 tagagggatt agaagccgtt agaaaaagac caggtatgta tattggtagt acaagcccta
     4261 gaggtttaca tcacttagtt tatgaagttg tagataatag tatagatgag gctttacaag
     4321 gatattgttc agacatatat gtatctatta atgaagatgg cagtgtttta gtaaaagata
     4381 acggaagagg tatacctgtt gaaatacatc ctaaaactgg taaatcaact ttagaaacag
     4441 tattaactaa tcttcatgca ggaggaaagt ttggaggcgg aggatataaa gtatctggag
     4501 gtcttcatgg agttggtgtt tctgtagtta atgcattgtc aaaatggatg gtagcagagg
     4561 tttatttaaa cggaaagata tataagcaga catatgagaa aggattgcca acatctaagc
     4621 ttgaagtagt tggagaatct caagataaag gaacaatgat tcaatttatg ccagatgaga
     4681 caatatttga tgaaatagag tttaaatatg aaacattgga gtataggctt agagaacttt
     4741 ctttcttgaa taaaggaata aaaatagtat ttgaagataa aagagaagga caagaaaaaa
     4801 gaaaagaatt tcactataca ggtggtttgg tagaatatat taagtattta aataaatcta
     4861 gaactggtat acatgatgac atagtttata tagataaaaa ggttgatgat tgttttgttg
     4921 aactagctat gcaatataca gatggttata cagagaacat atattctttt gcaaacaata
     4981 taaatacaca tgaaggtggt tctcatttaa gtggatttaa agcagctctt actaaaactg
     5041 taaatgatta tgctaagaga aataaatttt taaaggaaaa tgatgttaac ctacttggtg
     5101 aagacataag agaaggtctt acagcagtag tatcagttaa attaccagaa cctcaatttg
     5161 aaggtcaaac taaaacaaag ctaggtaata gctttatgag aggtatagtt gatagtgtaa
     5221 cagttgatga actggggtct ttcctagaag aaaacccatc aacagcaaga atcatagttg
     5281 ataaagcttt aagagcacaa agagctagag aagctgcaaa aaaagcaaga gaattaacaa
     5341 ggagaaaaag tgtattagaa agtacatctt tacctggaaa acttgcagat tgtgcagaaa
     5401 aagatccatc taaaagtgaa atattcttag tcgaagggga ttcagcggga ggttcagcta
     5461 aacaaggtag agatagaaat agtcaagcta tacttccatt aagagggaaa atacttaatg
     5521 ttgagaaatc aagactagat aggatactat cttcagatga aataaaaaat atgataacag
     5581 cttatggttg tggtattgga gaagattttg atatagataa ggctaggtat cataaaatta
     5641 taattatgac cgatgctgat gtagacggag cccatataag aactttatta ttaacattct
     5701 tctttagata tatgagacct cttatagatg aggggtatgt ttatgcagca cagccacctt
     5761 tatataaagt gacaaagcaa aagaaggaac attatgttta ctcagataaa gagttaaata
     5821 tcttactaga tgaaattgga agaaatggtg ttgaacttca aaggtacaaa ggtcttggag
     5881 agatgaatgc agagcaatta tgggaaacaa ctatgaatcc tgagacaaga actttattac
     5941 aagttactgt agaagatgct gcaattgcag atgaagtatt ttcaatgctt atgggtgata
     6001 aggttgctcc aagaaaagaa tttatagaag aaaatgcaag atttgttaga aacttagata
     6061 tatagagaga ggggattagt atacatggaa gaaaataaca aaatactccc tattgaaata
     6121 gcggaagaaa tgaaaaaatc gtatattgat tattcaatga gtgttatagc tggacgtgct
     6181 cttcctgatg ttagagatgg tttaaagcca gttcatagaa gaatattata ttcaatgagt
     6241 gagttaaatt taactccaga taaaccatac aggaagtcag ctcgtattgt tggggacgtt
     6301 ttaggtaagt accatcctca tggagatact gctgtttatt atgctatggt aagaatggca
     6361 caagattttt caactagagc acttttagta gatggtcatg gtaactttgg ttctgttgat
     6421 ggggattcac cagctgctat gcgttataca gaagctaaaa tgagtaaatt atcattagaa
     6481 ctactgagag atattgaaaa ggaaactgta gactttaaac caaactttga tgagtcgtta
     6541 aaagagcctt cagtattgcc agctagatat cctaatttat tagtaaatgg ctcaaatggt
     6601 atagctgttg gtatggcaac ttcaatacct ccacataatt tagcagaagt aattgatgca
     6661 actgtatatt tgatagataa tccagagtgt agtgtagatg atttaataaa atttgttcaa
     6721 ggaccagatt tccctacagc tgcaattata atgggaaaag aaagtatagc agaagcatac
     6781 agaactggaa gaggaaaagt taaagttagg tctagagctt ttatagaaga gctaccaaaa
     6841 ggaaaacagc aaataatagt tacagaaata ccttatcaag taaataaggc taaactggtt
     6901 gaaagaatag cagagttagt taaagaaaag agaatagaag gtatatcaga cctaagagac
     6961 gaaagtaata gaaatggtat gagaattgtt atagaattaa agagggatgc taatgctaat
     7021 atagtattaa ataatttgta taaacattct caaatggaag atacttttag tataataatg
     7081 cttgcacttg tagatggtca gccaagagtt ttaaatctta aacaaatatt atatcattat
     7141 attaaacatc aagaagatgt tgttacaaga agaactaaat ttgaactaaa taaagctgaa
     7201 gcaagagcac atattttaga aggattaaag attgctttag ataatataga tgctgttata
     7261 agcttgataa gagcttcaaa gactgggcaa gaagctaagc taggtttaat agaaaaattc
     7321 aaattaactg aaatccaagc acaagctata ttagatatga gacttcaaag acttacaggt
     7381 ttagaaagag ataagataga agctgaatat gaagatttaa tcaagaagat aaatagatta
     7441 aaagagattt tagctgatga aagattactt ttaaatgtaa taaaggatga aattacaata
     7501 ataaaagaaa attactctga tgagagaaga acagaaataa gacatgctga aggcgaaata
     7561 gatatgagag atcttataag tgatgaagaa atagcaataa ctcttactca ctttggatat
     7621 ataaaaaggc ttccatctga tacttataag agtcaaaaaa ggggtggaag aggtatttca
     7681 gcacttacaa ctagagaaga agattttgta aggcacttgg taactacaac tactcatagt
     7741 agactattat tctttacaaa taaaggtaga gtatttaaat taaatgcata tgaaatacca
     7801 gaaggtaaga gacaggctaa aggtactgct atagtgaatt tacttcaatt gtcagcagat
     7861 gagaaaattg ctactctaat acctattgat ggtaatgatg aaaatgaata tttattactt
     7921 gcaacgaaaa aaggtattgt taaaaagact aagagagaag agttcaaaaa tataaataaa
     7981 tctggtctta ttgcaatagg tttaagagat gatgatgagc ttattggagt agaacttaca
     8041 gatggaaaac aagaagtact tttagtaact aaagaaggta tgtctataag atttgatgaa
     8101 aatgatataa gatatatggg tagaacagca atgggtgtaa aaggtataac tttaagtaaa
     8161 gaagattttg ttgtgtctat gaacctttgc agtaaaggta cagatgtgtt agttgtaagt
     8221 aagaatggtt ttggaaagag aacaaatata gaagagtata gaagtcaaat aagagctggt
     8281 aaaggaatta aaacttataa tatatctgaa aaaactggta caattgtagg tgcagatatg
     8341 gtcaacgaag atgatgagat aatgattata aattctgatg gagttcttat tagaataaga
     8401 gtcaatgaaa tatcactgtt tggaagagtt acaagtggtg ttaaattaat gaagacgaat
     8461 gatgaagtca atgtagtttc gattgccaaa ataaatattg aagaagaata ggataaatat
     8521 cctattcttt ttgtataatg tagttagata aaaatttaaa attgaaagga gtaattgtta
     8581 tgtgcaataa aagaagttgt aaaaaaggtg gatttttatt attaggagct attttagggt
     8641 ttatatttgg tatgtttttt gcacctaaaa aaggttctga actaagaaaa gaaacaaaag
     8701 agaagtttaa tgatgtcaaa gaaaatccaa aagaagttct acatgaaaca tttaatgatg
     8761 ttaaagagag aataataaat ttagtggatg atgataataa tgaagaagat ataaagattt
     8821 cagaagaaga tatagtaata agtaaaagct ttgatgatga gggagatgtt aactaatgaa
     8881 tgcatggggg tggcaaattg gagcagtgtt agtaggaagc tctgctctta tagtagcaat
     8941 ttatttagct aaaactctaa acagcataaa taaggttgtt gaaaaggcat ataagattgt
     9001 agattataat gaaagacata tacaagatat tgttgaaaat gcatcatcaa tatcaaaggg
     9061 aatagatgat atagtttatg taattaataa aatattgagt ataggaaata tatttaaaat
     9121 tattaaaagg aaataggagg tttttacatg tctatgaata ttgattcaaa tttagattca
     9181 caaaataaat tttggaatgt ttgtcttgat ggagaacttg atgtttctac agcagataaa
     9241 ctaaaggaac atttacatgc tttaatagaa aaaaatatgt tagatgtaaa gattaatctt
     9301 aaagatttag attatataga ctcaacaggc ttaggagcta tgataggtgt attaaaaaaa
     9361 ttaaaaataa atgaaaaaga gatatacata gtaaatccta agagtaacgt aagaaagatt
     9421 tttactataa caggtctaga taaaatattt aaagtggagg gataaacttt ggcttgtgag
     9481 actataaaga tggaaattac tacaaatcca gattatgtta gtataataag attaacagca
     9541 tctggaattg ccaataagat gggttttcca atagatgata tagaagatat aaaggtagca
     9601 gtatctgaag catgtacaaa tgcaataaaa cacagtaaag atgatgtgtt taatatagta
     9661 tttaatattt tagataatgc aattaatata gaaattcaag ataatggaaa aggatatgat
     9721 gttagctcaa taagtacacc agatttagag aatccaaaag agagtggtct tggattgttt
     9781 ataattaaaa ctttaatgga tgatgtagat attgaatcag aacataatca agggacaaaa
     9841 ataaaaatga ctaaatattt aggagttgat atttaatgaa aaatgtagct aatgctacac
     9901 attacctaaa tatggataca aaagagctat ttaatttata taataaaaat aaaaatgtag
     9961 acattagaaa tattcttatt gaaagacatc tgtatttagc aagattgtta gctaagaagt
    10021 atataaacaa aggtgttgat tttgaagata tatatcaagt tgcttcattg gcattaattt
    10081 atgctattga tagatatgat gttgagaaag gatttgagtt ctccagcttt gcaacaccaa
    10141 ctatagtagg agaaataaaa aagtatttta gagataaggt atggacactt agagtcccaa
    10201 gaagaataca ggaattaagc aaaaagataa gtgatgcgaa gattaaatta gaacaagaaa
    10261 ataagaagca tccaaaggta aaagatatag ctgattatat aggcgttagt gaagaagatg
    10321 ttttagaggc tatggaggct tcttatggat atcaaccaat gtctttggat tcatcaagta
    10381 atgatgattc agaggataaa gatataactc ttattgataa gattggaaaa gaagaaggta
    10441 actttggaag tatagagtat gaagatttta ttaataagtt tattgaaact ttaaatgaat
    10501 tagaagttaa aatttttaaa gatagatttt tctttgataa aacacaatct agtatagcta
    10561 aagaactagg gatatcacag atgactgttt ctagactaga gaaaaaggta gtagagaagc
    10621 tgaaaaaaga atatgaaaaa aatttataaa aaaatataaa aaaagtattg acctactgtt
    10681 ttaaagatgg tatagtatta cttgtccttg aaaaaaggaa caagaaataa acacgaacat
    10741 tgaaaattaa acagtaggtt aatttatat
//
LOCUS       DAELTA010000125        10720 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.125, whole genome shotgun sequence.
ACCESSION   DAELTA010000125 DAELTA010000000
VERSION     DAELTA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.125"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            255..1376
                     /locus_tag="KPG13_002749"
     CDS             255..1376
                     /locus_tag="KPG13_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986449.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            1394..2269
                     /locus_tag="KPG13_002750"
     CDS             1394..2269
                     /locus_tag="KPG13_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG0986450.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            2293..2985
                     /locus_tag="KPG13_002751"
     CDS             2293..2985
                     /locus_tag="KPG13_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG0986451.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            2993..3817
                     /locus_tag="KPG13_002752"
     CDS             2993..3817
                     /locus_tag="KPG13_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0986452.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            3822..4889
                     /locus_tag="KPG13_002753"
     CDS             3822..4889
                     /locus_tag="KPG13_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG0986453.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            4891..5427
                     /locus_tag="KPG13_002754"
     CDS             4891..5427
                     /locus_tag="KPG13_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG0986454.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            5495..6100
                     /locus_tag="KPG13_002755"
     CDS             5495..6100
                     /locus_tag="KPG13_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG0986455.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            6112..7026
                     /locus_tag="KPG13_002756"
     CDS             6112..7026
                     /locus_tag="KPG13_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0986456.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            7068..8240
                     /locus_tag="KPG13_002757"
     CDS             7068..8240
                     /locus_tag="KPG13_002757"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG0986457.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            8287..9099
                     /locus_tag="KPG13_002758"
     CDS             8287..9099
                     /locus_tag="KPG13_002758"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG0986458.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            9303..10628
                     /locus_tag="KPG13_002759"
     CDS             9303..10628
                     /locus_tag="KPG13_002759"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG0986459.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT         4284 a         1048 c         2032 g         3356 t
ORIGIN      
        1 ttattatact atggatatag tatgtgtctt tacaagttgt aaactgacag tggtttattt
       61 tttaatataa atattgactt tgatgcaggt aaactttgta tttttaagcg tattatggaa
      121 tatgttaaat aaaaaaatga tgaaatatag tattgtaaat gccaaagatg caaaacaaac
      181 ttaaaacatt tattttattg ttaagtaatg ctataatata atgtgatttt aataatgata
      241 gtggaggttt aaatatgaga gtcgaggccc ctataaaagt agatcgaaaa accaaaaaac
      301 ttgctaaaag agttgaaagt ggggaaatag cagttataaa tcatatagac atagatgaag
      361 ttgctgcaaa ctctttagta gaagctaaaa taaaacttgt cataaatgcg gctccttcta
      421 taagtggtag gtatcccaat aaaggtccag ggatattaac tgaaaataat atactaataa
      481 ttgataatgt tggagaagag ttatttgaag aattaaaaga aggagaaact atagaagttg
      541 tagatggaaa gatatataga aaaggaaaat ttttaggtgc aggagaagtt ttagacaagt
      601 atgaggtttc acaccaaata aaggctgcct atgagaattt agctgtagaa ttggatagat
      661 ttatagataa tactatagat tatgctaaaa aagaaaaagg atttatactt ggagaagtag
      721 aaattccaaa agtgaaaact aattatgcta ataaacatgt attgatagtt gtaagaggtc
      781 aggactacaa agaagattta agtactatgc tttcttatat agaagaggta aagccagtat
      841 tggttggtgt tgatggagga gcagacgctt taattgagtt tggatatact ccagatgtga
      901 ttgtaggtga tatggatagt gtaagtgatg aagctttaaa aaaggctagt gaaatagtag
      961 tacatgctta tacagatgga agagcaccag gtcttaagag ggttgaggaa ttaggcttag
     1021 atgcagttgt atttcctgcg ccaggaacta gtgaagatat agctatgctt atagcctatg
     1081 agtataaggc tgagttaata gtagcagttg gtacacattc aaatatgatt gattttcttg
     1141 aaaaaggaag aaaaggtatg gcaagtactt ttttagttag actaaaaata ggttcaaagc
     1201 ttatagatgc taaaggcgtt aatttgttat acagaagtaa attaaaaata aagtacatat
     1261 gggcattaat agcaacagca ttatttcctg tgttggtagt tgcatcattg tcaccaggag
     1321 ttcaacagtt catacaacta atgcaattaa agtttagagt attattacag atgtaaattt
     1381 taggaggata aatatgcata ttaatatgaa atattatata gtcactatag gggctatatt
     1441 tatagctttg ggtataggca tgttagtagg atttaattta aataataatc aacaattaag
     1501 tgaacaacaa gcaaatataa taaatgattt ggatgataag tttaatatct taaaagaaaa
     1561 aaatgataaa cttgacagtg acttagcaag tgtaaataga gattatgaag aggcagttaa
     1621 ttttataaat aaaaatgtag ataagatttt agctggttcg ctgaatggta agagtatagg
     1681 aataatatct gctaatgaaa atgacgatta tacaaaaaat atagaagaca taataaataa
     1741 atctaatgga agcattgcat ttaatataat attaaaagaa aacataacaa atccagaaaa
     1801 gttaaaggaa atgtcaacta aactaggtac agatgtaaaa aatgcaaatg atgcagttaa
     1861 ttatataatt gacactctta aaaaggaaga tgcaagtgac attttaacat atcttcaaga
     1921 actagatgta ataaagttta attttattgg agatacatat ctaaaatatg attccgttgt
     1981 aatagctggt ggaaatgatg caaaagacag tacaaaacag tttgaaaaaa tagaaaaatt
     2041 tgtagtttca aaacttaaat cagagaataa atatttagta gaagttcaaa atacaggagt
     2101 taaaacttct tatgtagaac tatattctaa aaacaaagtt gcaactatag acaatattga
     2161 tgaaggtata ggaagtatat ctctagcgat tcttctacaa caaggtaata tagttggtaa
     2221 ttttggtaga ttggatactg caacttcatt gttgccatct attaaataga aataatttta
     2281 aggagagaaa aaatggattc ttatataagt ataataatac cagcttataa tgaagaaagt
     2341 agaataaaat atacattaga aagcatattg gacattaagc aaataaatga aataatagtt
     2401 gtggatgatg gttcaattga caatactgct aaggttgtat ctgagctaga aaatgaaaag
     2461 attaaatttt ttaagttaga taaaaataga ggtaaaggat atgctttaaa ttatggtatc
     2521 aagttagcaa tgaaaaatgc agatattata ggttttctgg atggagattt aggaagttct
     2581 gctaaagaag ttgaaaaatt aatagtacct atattaaatg gtgaggcgga tgttacaata
     2641 gccaagtttc ctcctgcaaa gagaaaagga ggtttgggat ttgtaaaagg tttagctaaa
     2701 aattctgtgt ttgagatgac tggtgttgag ttagatgcaa ctttatcagg tcaaagaata
     2761 tttaaaagag aagttttaga aaagtttgat gaaatacctt ttggatatgg agtagaagtt
     2821 ggaatgacca ttgatatatt aaagtatgga tttaaaatta aagaagtatt agtaaatatg
     2881 actcataatg aaacaggaag aaatttaaaa ggttttattc atagagggaa acagtattat
     2941 cacataaaaa aggttttagg acaaaaaaag aaagaatgga ggtaatatct tagtgaaaga
     3001 ttatatactt tataccattt tattattaac tggattattt ggaacctatg ctgtaattcc
     3061 actatttaaa aatttattga taaatggaaa tgtacttaga ccaaactata aaaaagatat
     3121 gataccagta agtatgggaa ttgtattttt gcctatgatt gtaatcaatg gaataatact
     3181 tgctttcttt actacagaat ttaagaattt agcgtatata tttatgttta tgtttggtat
     3241 gatatccatg ttttttgcag ggatactaga tgatgtcatt ggaaatagag atgtaagtgg
     3301 tttaaagggg cattttaaaa gtttatttaa aggaagtctt acaacaggag gttttaaagc
     3361 tttatttggt ggatttgtag ggcttatagt ttctgttgct atatcaaaag acataattga
     3421 tataatagta aatacactaa taatagctct atcaactaat ttaatgaatc ttttggattt
     3481 aagaccagga agggcaataa aagcatattt attcataatg gtaattgtat ttttaacatt
     3541 gacaggattt gttcaagttc ttccattact tatagtacca aatgtacttg catactttaa
     3601 ttatgattta aaagcaaggg ctatgatggg agatactggt tcaaacgtcc ttggaatatc
     3661 tattggaatg ctcatttctt ttgggtatcc atttaacatt agattaggat ggttaatatt
     3721 tgtagttatt atgcatatat tcactgagaa atactcatta actaagataa tagaaaaaaa
     3781 taaactatta aattttatag ataaattggg aaggtagtat tatgataagc aaaagagtgg
     3841 ggattgttga atcaatagta agtcaaactg aaactctaga tgatataaga gtaaatataa
     3901 atggagaaat acaaaaggca tataattatc caaaaatatc tggaactata aatataggtg
     3961 acgaagtggt tttaaataca acagcagtag aactgagctt aggtacaggt ggataccatt
     4021 ttgtaataac taatttaaat aatatagaat ctactttaac tgaaggtgga catataatga
     4081 aacttagata tacgcctctt caaataaaag tggattctgt tgaggaacaa gaaagtattt
     4141 atcatgataa atttgcgaat tttaaaggat tagaaggtct ttctgttgta gtagggactc
     4201 ttcatagtat gttaactcca tttgcagctt catttaaaag aaataatcca aataaaaaat
     4261 tagtgtatat aatgactgat ggagcatctc ttccaattta tttaagtaaa aatgtggata
     4321 ctttaaaaga aaaaaaatta atagatagta caataactat aggaaatgcg tttggtggag
     4381 attatgaatg tataaatata tatactgcat taattacagc aaaagaaata ctaaaagcgg
     4441 atgcagtatt tgtcagtatg ggacctggaa ttgctgggac aggaacaaaa tatggattta
     4501 cagggattga acaaggctct attcttgatg ctgtaaaaaa acttgaagga agagcaattg
     4561 caattccaag aattagtttt gctgacaaac gagaaagaca tcaaggtatt tcacatcata
     4621 gtatgactgt atttaaagaa attgtaaatg taaatgtaga cataccaata actatatatg
     4681 atgatgaaaa attgaacttt ataaaagagc aaatcaattt aaatggttta gacaaaaagc
     4741 ataatataat ttatatagat aataataaaa cgaaagagga tttagactat tttaatctaa
     4801 aagtaaagag tatgggaaga aattatgaac aagatgaggc atttttcaaa gctgcaagca
     4861 cagcagcata ttacttaatg gaggtttaag atggtattag aagaaaaaac tattagtagt
     4921 gatagggttt atacaggtaa agtaattact ttaaaggttg atactgttga aattccaggg
     4981 caaggatatc aaaaaaggga gttagtagaa gttggtggag cagtaggaat agttgcaatc
     5041 acagatgata ataaagttgt gttagttaag caatttagaa aacctataga aaagccaatc
     5101 tttgaaattc cagctggtaa actagaaaaa aatgaaagtc caaaagaatg tgctgaaaga
     5161 gaattaaaag aagaaactgg gtatagtgca aaaaatatta agctaataca taaatttttc
     5221 acttcagcag gtttttctaa tgagataatg tttgtatatt tagcaacagg tcttactcca
     5281 ggagagaata atttagatgc agatgagttt ttagatgttt atgaaattga gcttgaagaa
     5341 gcatataaca tggtattaaa aaatgacgtt gaagatgcaa aaacatctat tggattatta
     5401 ttagtaaaag atatgtttaa aaattaatat ttataaaatg aataaccctt tgtcctaaag
     5461 catattattt tataaaggac aagggggaat taaattgaga agaacatata gaaaaaatga
     5521 ttttagagaa ttaaatagac aaataattat tataggattg ttgtttatga tgtctattgt
     5581 tgttgggtct tacataaata aaatattgcc aggttctagt aacaatatat taaacaatat
     5641 aaatcctgct gtagagtatt ataatctaaa tataagcata aaagatacag tgatacaaaa
     5701 tttaaaatca gatgcaatct ttatgggctc aatagctcta ttaagtctat ttgtagtaac
     5761 aattccagca gtgttggtag cttttgtatt aaaagggatg tcaattggtt atacaataaa
     5821 tagttgtata ttggcattaa aatttaaaag tataaaaatg attttaataa tattatttaa
     5881 aaatttgatt attattccat gtgcaatcat attagcatta attagtttaa gttattttaa
     5941 agaaatggtt tatgaattta aaaagaaaag tagaaaaaat atgcaatttt taattaagag
     6001 gtatatacta aatattataa ttataatagc actatcattg gggttgcaat taatattaaa
     6061 tactgcaagt attggtatta ttaagttcct agctaaataa aggagtttat aatggatatt
     6121 atagagggat atatagatta cataaaagat aaaaaaaaat tatcagagaa tacagtggca
     6181 tcatacttta tggatataaa aaaatatatg gaatatataa atcaaaaaag cattaaacta
     6241 gtagatatag tagaaaatga tattataagt tacttaataa agttggaaaa agataatgta
     6301 tcaatagcta caatagcgag gatgatatcc tctataaaat cttttcatga ttatttattt
     6361 ttaaatcata tatgtacaaa taatcctgca aaggatataa aaaaacctaa aataaaaaaa
     6421 gaaaatataa atatacttac agaagaagaa attgaaagac tattaaattt tccaaagtta
     6481 acaacaccta agttgattag ggacaaggct atttttgaag tattatatgg tactggtata
     6541 aaagtatctg aattagtaga gatgaatata gaggatattg acctagatat tgattatata
     6601 tattgtaatt catgttgtaa aaatcaaaga gtgataccac tttgtgatat tacaaaactt
     6661 tacttagaaa agtatctaaa agaagcaaga cctaaaatgg ctgcagaagg tgaaaaatca
     6721 ttattcgtaa gttcattagg tcaaaggttt acacgacaag gtctttggaa agtcataaaa
     6781 aaatattcta atctagccaa tatagataaa aatataaatc ctacaatgct tagacattca
     6841 tttgctattc atttgttaaa tgaaggtgct aacattgctg ttgtaagtaa aattttagga
     6901 aatgttaatt tatctagttt gcaagtatat ttaaatcata tagataaaaa cgtgagaaga
     6961 gaaataaagg agaaacatcc tagaaatgat gttgatgtag aattacaaaa agcagaagct
     7021 aaatagacta tatagcaaat tttataattt agaaggagca tatttatatg agcagagtaa
     7081 tttggattgt aattgatagt gttgggatag gagcattacc tgatgctgaa aaatttggtg
     7141 atagtaagga tgtaagtacc cttggaaata tatttaagga atatccagat attcagattc
     7201 ctaatatgag aaatttaggt ataggaaaca tagaaggtat agattttttt gaaagtataa
     7261 aagaaccaat aggttgtttt ggaaagtgta aggaaatgtc tcagggaaaa gatactacta
     7321 caggtcattg ggaaatgact ggtataatag ttgacaaacc atttaagaca tttgaacatg
     7381 gattctctaa ggagataata gaagagtttg agaaaaaaac tggaagaaaa gtagtaggaa
     7441 ataaaccagc ttcaggaact gtaataatag atgagtatgg agagcatcaa ataaaaactg
     7501 gagatgtaat agtgtataca tcagctgata gtgtatttca gattgctgct aatgaagaag
     7561 taattcccct agaagaactc tataatatgt gcaaaatcgc tagggaaata atgatggggg
     7621 ataatgcagt agctagagta attgctagac cattcatagg taagaaaaaa ggtgagtttg
     7681 tgaggacttc aaatagaaga gattattcac tagacccatt tgaaccaaca gttcttgata
     7741 atataaaaga atcaggactt gatgttttag cagttggaaa aatagaagat atttttaatg
     7801 gaaaaggaat tacagatgct atccacacta aaagcaatat ggatggagtg gatgaaactt
     7861 taaattatat gaaacaagat aataaaggtc taatatattc aaatctcgtt gattttgatt
     7921 ctaaatatgg tcataggaga gacccagaag gatataagaa agctcttgaa gagtttgata
     7981 gtagacttcc tgaaataatg gctaatatga gagaagatga tattttaata atcaatgcag
     8041 accatggaaa tgacccaaca tataaaggta cagaccatac aagagaatat atacctgtta
     8101 tgatttatgg caataagatt aaaaaaggtt ttaatttagg agtaaaagat acttttgctg
     8161 atattggagc aactgttgca gatatactaa atgtaaagtt gcctaaacat gggtcaagct
     8221 ttaaagaaga tttattttaa actataaaat gataaaaact tagcttaata ggatggagag
     8281 attggaatgt ttgaaaagat agcacaaagt agtaaattta taaattctaa aagtaatata
     8341 aaacctaaga ttggtttgat attaggttct ggactaggtg atttagcaaa tgatatagaa
     8401 gaccctgtca caattaaata tagtgaaatt ccaaacttcc ctgtatcaac agtagcagga
     8461 catgctggac aacttgtaat tggaaaactt gaaggaaaag aagttattgc tatgcaaggt
     8521 agatttcatt actatgaagg atattctcag aaagaagcta cttttccagt aagggttatg
     8581 aaagaattag gagtcgagat tcttatagta actaacgctg ctggaggcgt aaataaagaa
     8641 tttaaagcag gagatttgat gcttataaga gaccatataa actttagtgg aagtaatcca
     8701 ttagtaggaa aaaatgatga cagatttggt gctagatttc cagatatgtc agatgcatat
     8761 tcttctaaat atgtagatgt agttaaaaac tgcgctaaag aatgcaatat agatgtcaaa
     8821 gaaggcgttt atatgttttt tagtggtcca aactatgaaa caccagcaga agtgagaatg
     8881 gcacaaattt tgggagctga tgctgtaggt atgtctactg ttccagaagt catagtagct
     8941 tctcactcta atataggagt tattggtata tcttgtataa caaatatggc tgctggcatc
     9001 ttaaatcaac cactaagtca tgaggaagta atagaaacta ctcaaaaagt taaatcagaa
     9061 tttatgaatt tagttaaaac aacggttaaa aatttataag ttaaagctta ttatagattt
     9121 tttgaggaag tttataataa aatagttaat gtttaatttg aaaagaatta ataaaaagct
     9181 gtcttgatat agaaagttat ttctaataaa ggacagtttt tttataatac tacaacattt
     9241 gaaagtgttt taaagtttaa tatttattta aattattata ggttagaatg gagatttcaa
     9301 ttatgagaat gtatgatatt ataaaaaaaa agagagataa cctagagctt agtaaaaaag
     9361 agataaacta ctttatagaa aactattcaa agggagatat acctgactat caagcttctg
     9421 cactattaat gtcgatttat ttaaataaga tgaataaaca ggaaactgta catcttacag
     9481 aagcaatgat gaactctgga gacatgataa acctatctga gatagatgga ataaaggtag
     9541 ataagcatag tactggtggt gttggagata agactaccat agctcttata cctttggttg
     9601 cttcatgtgg agcaccagtt gcaaagatgt caggtagagg tcttggtcat actggtggta
     9661 ctattgataa attggagtct ataccaggtt tttcgacaga aatggagata agtaaattta
     9721 ttgattctgt aaataagtct aaaatagcag tttgtggtca aagtgcaaag gtagcaattg
     9781 cagataagaa aatatatgct cttagagatg ttacagcaac tgttgataat atatcattga
     9841 tatcatcaag tgtaatgtct aaaaagttag cttcaggagc tgatgcaatt gtgctagatg
     9901 taaaaactgg tgatggagca tttatgaaga cattagaaga gtcttttgag ttagctaaat
     9961 ctatggttga tataggtact ggtatgaata gagatacaat agggattgta acagatatgg
    10021 atgaaccttt aggttttgca gtgggaaact ctttagaagt tatagaggca attgagacat
    10081 taaaaggaaa tggacctaaa gaccttgttg agctatgcga agtattagga gcatatatgt
    10141 tggttttatc aaaggtagca catgattttg aagatggtgt tgaaaaaata agagaatcaa
    10201 taaaatctgg tactgctata caaaaactca aagagtttat agagaatcaa ggtggtaata
    10261 aagatgtagt agatgattat tctttattca aaaaatcaaa gtataaatat ccagttaaat
    10321 caactaaatc aggttatata agcaaaatta aagctgaaga tgttggtgta tcggctatga
    10381 ttcttggagc aggtagagaa actaaagatg atattttaga tttatcagca gggataattt
    10441 tagaaaagaa agtaggagat tatgtaaatg aaggtgaagt tttagcttac atgtattata
    10501 atgatgaaca aaagctttca ttggcaaagg gaagatttat tgattcctac tctattgtag
    10561 attcaaaaat agaaaaaaat aaattaatat atggtattgt aactaaagaa gagattaaaa
    10621 agttttagaa aattttagaa aagtgattat aaaaggccta acatgtcaaa aataatgtta
    10681 ggttttttat tttttgataa actataaatt ataccaaaaa
//
LOCUS       DAELTA010000126        10652 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.126, whole genome shotgun sequence.
ACCESSION   DAELTA010000126 DAELTA010000000
VERSION     DAELTA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.126"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..442)
                     /locus_tag="KPG13_002760"
     CDS             complement(<1..442)
                     /locus_tag="KPG13_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG0986460.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNI"
     gene            complement(1046..2203)
                     /locus_tag="KPG13_002761"
     CDS             complement(1046..2203)
                     /locus_tag="KPG13_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG0986461.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            2357..2926
                     /locus_tag="KPG13_002762"
     CDS             2357..2926
                     /locus_tag="KPG13_002762"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG0986462.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(3108..3524)
                     /locus_tag="KPG13_002763"
     CDS             complement(3108..3524)
                     /locus_tag="KPG13_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986463.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(3750..4844)
                     /locus_tag="KPG13_002764"
     CDS             complement(3750..4844)
                     /locus_tag="KPG13_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986464.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            complement(5267..6208)
                     /locus_tag="KPG13_002765"
     CDS             complement(5267..6208)
                     /locus_tag="KPG13_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0986465.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            complement(6201..7028)
                     /locus_tag="KPG13_002766"
     CDS             complement(6201..7028)
                     /locus_tag="KPG13_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0986466.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            complement(7199..7582)
                     /locus_tag="KPG13_002767"
     CDS             complement(7199..7582)
                     /locus_tag="KPG13_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG0986467.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            complement(7551..7838)
                     /locus_tag="KPG13_002768"
     CDS             complement(7551..7838)
                     /locus_tag="KPG13_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG0986468.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            complement(7871..9028)
                     /gene="alr"
                     /locus_tag="KPG13_002769"
     CDS             complement(7871..9028)
                     /gene="alr"
                     /locus_tag="KPG13_002769"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0986469.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            complement(9043..9633)
                     /locus_tag="KPG13_002770"
     CDS             complement(9043..9633)
                     /locus_tag="KPG13_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986470.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            complement(9654..10115)
                     /locus_tag="KPG13_002771"
     CDS             complement(9654..10115)
                     /locus_tag="KPG13_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG0986471.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            complement(10130..10510)
                     /locus_tag="KPG13_002772"
     CDS             complement(10130..10510)
                     /locus_tag="KPG13_002772"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG0986472.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
BASE COUNT         3427 a         1934 c         1114 g         4177 t
ORIGIN      
        1 ttatattgtt tattactttt ttaaatccac tagctgtaat aataaatgtc ttttctggta
       61 tccctggtga aaagattatt ttatcatggt taaatatatc ttgatctatt acaagagaga
      121 tatttttctc atatccaaaa ggagatacac atccaggcaa acattgtgtt ttttgtgtta
      181 attcttcttt tgaacatata gatacttttc cacctaaaac attttttata gtttttggat
      241 ctaatttttt tccttctgtg gttacaaata tatagtaatt tccagatcta tcttttataa
      301 ataaactttt actttctact ccagtatatt gtaatttttc tcttatttct ttagcttttt
      361 caaatgataa aataggttca tggtcatatt cttcaaacgt tatatgattt tcttttaaaa
      421 tatttttgat attgttgtac atatattttc ctttctatat ttaaattaat ttatatataa
      481 tattcaatta taaagtgatt ttataatttc ttcaaactat cataaaaaac attcacatgt
      541 tttaggaata aattatataa aataggttat aagataatga acatttatat tataacctat
      601 ttttatatcg ataagtatga aattgtttaa ccaagtcaaa atgatatata aatttatatt
      661 taaatgtgtt tagagtttaa tttattttca atgtttaaaa aagttattct atgcatctaa
      721 tttaattagt aaactgaggt tctagttaga taaattatat tataatgaat atatagtatg
      781 cagtttacga tattttaaaa gtaaatgata taatatttgg aacaactggg ttgcaatgtg
      841 tttttaaaaa atatcaaaat aaggctatga tttttaaaac catagccctg tctgtaaaaa
      901 tttaattatc acttaaaaaa taaattttta tttaaagaat acacataaat atttccagac
      961 tccctcatga actaaaaagt tcattttgag acatctcttt tataatgttc tttattttat
     1021 agtataagtt tataaaaatt aaatcctatt tatcaatctg ttctctaagc cacttagttg
     1081 ctacatctaa ctgtttattt tcatcttttt cttcaatagt aggctttata cctttttcat
     1141 tgatataatc tccattaggt gtaaaatatt gagcagtagt taacttgtat cctgttccat
     1201 ccttcaatct tacaactgat tgtaccaacc ctttaccaaa agttgtagtc cctacagaaa
     1261 taccttcttt attatcaacg attgcagctg ttaatatttc agcagcagaa gcactttctc
     1321 cattagttaa cactactatt ggaagtccca atttttcttt atcagatttc aagtactcag
     1381 tatttccctt attatctttt gtatatacta tagttccttc accaattaac tcatcagcaa
     1441 cttctttaca tatatctagc attccaccag gattatttct aaggtctatt actaatccct
     1501 tgatattttt tgcttttaat tcttttaaag ctttatcaaa gtcagtatat gtacttgata
     1561 taaattgggt aatttctata tatcctaaat cattatcaat tactttactt tctatactct
     1621 tctcaattat ctggtctgtt tttactttaa agttcaattg tttatcttct cttaatatag
     1681 tcagttcaac tgttttacct tttttaccct tcatcatacg aacagcttca tctgaatttt
     1741 tatatgagac agattttcca ttgactttta ctaatttatc ccctgatttt acacctgctt
     1801 tctctgctgg agaatctttc atgggaacta ctactaagcc accatctttt gatgccccta
     1861 tatacatacc tacaccaaca taagaacctt cactatcatt aattagattc tccatttcat
     1921 ctttagtata ataaccagaa tacttatcat ttgttcctag aaatagacct tttgctgctc
     1981 catttaccaa atcagtatct gatacttttt tataaaaatc ttgttttata atagattcta
     2041 gtcctaacaa cttatcatat tttttatagt cttcatagag ttctttcgat attacaactt
     2101 tattccccaa tgtcaactga aatgctgatg taaccatagc tgttataatt actaaaatca
     2161 cccctaaaaa aatagctttc ttctttgaaa tcacaagata catctccttt tacataaact
     2221 attattatta tatgactagc tgttattttt attacattta cccaaatata ttgctttatt
     2281 acaataaatt atactaaaat ttatattaaa tacaattgct gtacacatta taacatatta
     2341 agggaggaat tattttatga aaaaagaatt tagaaaaaaa gttattgaat caagaaaaaa
     2401 taagagtgca aacttcgtac ttagtaattc aaatttaatc actgaaaaat tacttcaaat
     2461 ggaagatata agaaatgcaa ctaatataat gctttatcta aattttaaca atgaagttca
     2521 aacagataat ttaatcaaaa aacttttgtc tctaaagaaa atagtatcaa gtcccatcac
     2581 aataaaagaa aatcatacat taatccctac tcaaataaca gatttaaaag agggattgag
     2641 aattggagca tatggaataa gagaacctaa cgaaaaatca cctacaattg atattaaggc
     2701 cttagatgtt gtaattgtac cagctgtagc atatgatata cattgctata gattaggata
     2761 tggtggagga ttttatgata gatttttaga aaacttaaga aaagatgcta taactatcgg
     2821 catagcattt gacttccaga tattcgattc tattccaaaa gaaactcatg atgcacaatt
     2881 agactacata gttactgaaa ctagaattat cactccaaat aattagaata ttaagtattt
     2941 ccagacccca tttaatatag taataaatac tactgttaat atcttacttt ataagcattt
     3001 taagtcatgg cttttacaac aataagaacc atatacaatt tacttacatt aataaaaacc
     3061 atgactttat tgtacaatat gcagttattt aattataaaa taccttctta tttcgtcgca
     3121 tttaattcta ttctatctgg aagttcactc atatttgttt caacaactat gtatggaaaa
     3181 gaatcttcct tatctttata ctttatcttg actctcaaaa catcctttcc ttcctcaacc
     3241 acactttcta ttttgtccat ttctatacca tatttattat ttcctctagt cactataatg
     3301 tatattttgt tatcgatttt agccgctaat gctctttctt catttgtata cttatccatc
     3361 acatctaata tcttttctgg tattgaattt ctttgtaata ctgtataatc tatgggttta
     3421 gatttctcta caaacatact tggtatgaat ataaatcccc ctataacgaa tacaagagct
     3481 ataactccat aaattatacc tttaatatta aactttacat tcaatcttat cactcctccc
     3541 atatattcta aatatatgat ttataaactt atcttagact ctaaattatt gattagtata
     3601 tatttataca taattataaa tatatgtaat gtaactaaaa aacatgtttt ttctatttca
     3661 taataaacga gctaaaaaat aaaaagagct atatgaaaac attttcattg ttttcatata
     3721 gctcttgagt ttacaatatt aaaaaatact tatttgtcac tgattccttc ttgatatcca
     3781 gatgtttttg taatctgtct ataaagaaca caaaccagta ttatgacgcc catatatcca
     3841 gtatatggat ataaaatacc aaccagctta tcaaatggta ggaagccacc tataagacct
     3901 aatatagctg ctattaaagt tattaattta aacttaggat gtttatcatc tacaaatcta
     3961 ttagttactg accataataa aggtacagct gttgtatata tacctaatat aagaattatt
     4021 gagaataaaa ctccaattac tggtgaaatc ttatctgcta agaataatga tggtattgct
     4081 tttgaatata agtttcctat atcagaaaga atagctaagt gcatcattat agctgcagcc
     4141 ataagtgcta ctccacctaa tataccacca caaatagctt cttttttgtt agcagcactt
     4201 gctcctaatc cagttaagaa tgccataaca acaattgtgt tataagtagt atatattaca
     4261 ccagatagat aggcatttgg aactggttta gctatcttta cagtgctaag aacttgtgca
     4321 gcttctccta tattacctat gtttttagct aaacttatac caccaactaa aagtgtaaag
     4381 attattatta taggccctat tccacctagt atctttgata atttatcaag acctagtgaa
     4441 acagttaata atgctactac tgccatacct actctaccta caaatggatt taatccatag
     4501 tattcagtta aagttgctcc tgctcctgaa atcattacta cgaaaacacc aaataagaat
     4561 aaaggtccaa accattcaaa gaatattcct aaatacttac cacaataaac acgataaatt
     4621 ttgataggtt ctttaagttg aagctcttta ccttttgaca taactgaagc acccatccat
     4681 gagaataata ccattgaaat tatcccacca ataatacctg caaaaccata aaaagagaag
     4741 aattgcatta tttcctgtcc tgttgcaaat ccagaaccga ttactgttgc tacatacgca
     4801 cctgcgaaac tgatgactgt tttaacattt actttttctg acatatctca gttacctccc
     4861 catgtgaaat tttttcttct gattaaacaa ttcgataaac tacctacaat ttcctttaac
     4921 ataaaataca tgataactgt tttatagctt tttagtataa taacttaaaa cttataatct
     4981 gattttatac cctactcctc ctctcataat ttttttagat tcttttatct aagtagtcat
     5041 tatatataaa attagtttct ctataaatta tatattagtc atatttttct gaatagtcaa
     5101 tatatatttg aatattttct ctatttttgt aaaaatttga atattttatt acatttttga
     5161 tatattgata tcatataaaa aaaagagaga tataaattaa tataacactt tatacctctc
     5221 ttttaaatat attatattat taacaaacat gaataattta tatctcttac attctttttg
     5281 caataataac ttctttaaca gtctttatta tgtcatcaca agttaattca tattttttta
     5341 acaattcatt tggagtacca gattctccaa atgtatcttt aattccaact ttctttacaa
     5401 tggttggaca agactctcct actacctcac ttacagcaga acctaatccc ccaattatgc
     5461 tatgttcttc tacagttaca atagcattcg tttcttttgc tgattctaat attaaatctt
     5521 tgtctatagg ttttatcgag gacatgttta taactctagc acttatacct tcttcttgca
     5581 acttttcttg tgctaataga gcttcattta ccataattcc acaagctata attgatacat
     5641 cctttccttc tcttaagact gtaccttttc ctatttcaaa tttataatct tcatcaaata
     5701 aaactggtac tgaacttctc cctaatctaa cataaacagg tccattatac ttagctattt
     5761 caaaaataat tttttctgtt tcaactccat cagctggaac aagaactgtc atatttggta
     5821 tagctctcat tatagcaata tcttccacac tttcatgaga tgctccatcc tctccaacag
     5881 taagacctgc atgagtagca catattttta catttagctt tggataacaa actgaatttc
     5941 ttataatctc aaaagccctt cccgtagcaa acatagcaaa agtacttgca aaaggtattt
     6001 taccagcagt tgataatcca caagcagcgc ctattaaatt ttgttcagct atacccatat
     6061 taaaaaatct atctggaaat gacttgtaaa aatcatgtgt ttttgtagac tttgataaat
     6121 ctgcatctaa tactacaaca ttgtcattta tttgacctaa ttttactaat gctttaccat
     6181 atgcttctct cgtcgccatt ttactcattg tcagcacctc ctaattcaag caaagctttt
     6241 tctagctcct catcacttgg agctgtccca tgccatcctg cttgattttc cataaaagat
     6301 attcctttgc cttttatagt ttttgcaact atcattgtag gttttttaac tgttgattta
     6361 gctatatcaa gagccgcaaa tatttggtca aaatcatgtc catctatctc aattacattc
     6421 caaccaaaag ctttaaattt atcaactatt gaacctaaac tcattacgct ttctatatct
     6481 ccatctattt gaagtccatt attatctaaa aaagcaatta aattatcaag tttataatgt
     6541 gcagcactca tagcagcttc ccaaacaatt ccttcttgaa cctcaccatc accaagcaaa
     6601 gtatacacat tccacggagc attatccaac tttgatgcca tagccatacc acatgcaact
     6661 gaaaaacctt gacctaaaga accagtagat atttccacac ctggcgtgcc tttcatgtct
     6721 ggatgacctt gtaacatcct tcctatcttc cttaatccat tcagttcttc cttatcaaaa
     6781 tagccttttt cagccaatgt agcatataat gctggtgcag catgtccttt tgataataca
     6841 aatctatctc tatcctccat tttaggattt gaagaatcca cattcatttc atcaaagtat
     6901 aacgcagtaa gtatttcaac tactgataat gaccctcctg gatgtccaga tttggctcta
     6961 tgtatcattg aaactatatc tcttcttatg atacgcgcaa tctcatttaa tcctttatgg
     7021 tctctcatgc aattacctcc cttgagttcg taattttata tcaattccat attattatat
     7081 cacttttatg ctattttttt gcgttatttt taattttatt aacatagtac attaaaatta
     7141 aaccttaaga gatatttaaa agaaaaaagg gattgtctaa taaaagacaa atccctcttt
     7201 aaaaagtaaa caatcctaaa cttatttgaa gtccatcatc tacttttatc atcatatttt
     7261 catctagaca accaattttt tccctcagtc tctttttatc gatagttcta atttgctcta
     7321 aaagaattac tgagtcttta ttaagcccat attcattgga acttatctct acatgagtag
     7381 gtaatttggc tttattaatc tgagatgtta tagctgatac tataactgta ggactatatt
     7441 tattacctac atcattttgt attatcagaa caggtctaac gcctccctgc tctgaaccaa
     7501 caactggact taaatcagcg taatataaat caccacgctt tatttcacaa ttagtactca
     7561 ccttgggcaa tcttcccttc atttttatca tcagaagata cttcttcaac tgtcatacct
     7621 aattcagcta gtgacagatt aatttgaccc atttctctat aacccgtttt catagattct
     7681 ttaatatgaa ttttcttttt ctgagttata tagaactgaa aagcagcttt tgcaaaatct
     7741 tctctgttac tgttctccat ctgaactata ctatcaactt cagaaatcag agagtcaggt
     7801 aaagtaactt ctattttttc tttactctta tcgtacacaa aatcgcctcc cagattataa
     7861 tatcatggtt ttattttagc aaataactgt ttatttgtac aagctcatta ttttccatat
     7921 aaacacggtc aactcttctt gatatcatac ataacacttc atagtttata gttcctaagt
     7981 ctttagctat acgctcagct gtaacttcgc cttctccaaa tagtataacc tcatctccaa
     8041 cttttatgtc tatatctttg tcaattctaa ccattatttg atccatacat attctaccaa
     8101 ctacatcaaa cacttctccc ttaataagaa cctttggatt tttttggatt ctagtaaatc
     8161 catctgcgta tcctattgga actgtagcta ttgtttcttt acctgtagtt gtgtatttta
     8221 gtccataact tattcctaca cctggttcaa cttgttttat atgtccgatt tttgatttta
     8281 atttcatggc tggtcttaat tctaatctat ctttaaatac atcatcagat ggataatgac
     8341 catataatat tattcctgct cttaccatat ttaatcttaa atcagggcaa tccataatag
     8401 ctgcactgtt tgatacatgt tttatagcta tttttacacc agcctcatct aatttatcag
     8461 acataaattt ataattatta gcttgtttat aagtgtactc tttacttact tcatcagctg
     8521 tagcaaaatg agtaaacata ccttctaaat ctatatattc taatttattt aattctatta
     8581 tttcctgtac tgacttctca ttaggttgga aacctattct agtcatccct gagtctattt
     8641 taacatgaac acaggctttt tctcctaaag attttgcaat ttcatttatc ttttgtgctg
     8701 tttctaaaga ataaactgtc atagttattt tattttttat agaatcttca aaagcttcgt
     8761 ctggagtata tcccaagttc aaaataggaa gtgttatgcc attttgtcta agttcaattc
     8821 cttcagcagt tcttgctact gctaagtaat ctactttttc tttttctagc aattttgcaa
     8881 cttctactgc accatgtcca tatgcatcag cttttattac tccacaaatc ttaatatctt
     8941 cttctaataa atttttaata ttatttaagt taaatcttaa gttatctaga tttatctctg
     9001 cccatgtagg cactgttatt ttttgcatgt ctttagtccc ccttagtttc tgtattcaaa
     9061 atctttgtat tttactgtaa atcttggcac tccctcttta tctaacactt ccattttttc
     9121 aggatttttt gtgtcagcat ttacatataa tacttgcttg ttaaaatatt tattatcccc
     9181 aggaataaaa gtctctaaaa ccaaatgtcc tttggataac tttactttca tctcctcatt
     9241 ttgaagatag ttttttataa agtctcctac aaaaagatat tgattatttt ttcctgtatt
     9301 aggtaactcc actacatcac taatctttgg atttttaact aatattttat catcttgata
     9361 ttctatactt ttccctttta aatgttttgg ggaaattact tctaatttat agttatcggt
     9421 ttttttataa gtatgaatta aaacataatt gtgtgggctt ttatttccaa ctacttctac
     9481 ttcagcttta caactgtaat aattcatatc agaaatctgc ttttgaaaat ctttgtatac
     9541 ttcttctttt gtggactgtc gtttttggca tcctattaca attataacca gtgccaagaa
     9601 cataatacat actatggtcc acttttttct cacatcacac ctcctacttc agtttattca
     9661 ttgtataaag cttttattat atcatatcta caaattatac ctttaagttt gttgtcatca
     9721 tctactacag gaactctgtt tattgatttt ttaatcatta cattagctac atcatcaaac
     9781 ttatcatctt catgaacctt tattatatct ttagacataa gttctccaac cttataagca
     9841 gctacttgtt ttatatcttt ttcaactttt tttaagtcat ctaagaaaat caatccttgt
     9901 aatagattta tatagagagg tgcttctata tatttttctt ttttaagaat atctgtttca
     9961 gaaattattc caacaactct attctctgaa tctacaactg gcagacctcc tattttttct
    10021 gcaattagca tattagctac atctgctata ctatcatctt gttttgctac tattacatct
    10081 gttgtcatta tttcttttgc tgttttatcc atcataatgt aatcactcct tagtccttaa
    10141 ttatggttat cgcatttgct atagcataat cttttgaatg tgatattgaa acttttattt
    10201 ctaaaacatt ataatctatg cacatttttg ctagattatt ataagttttt actattggtt
    10261 ttcccatttc atttctgaga acttctatat ctttaaaatt aaacaatcta attcctgtac
    10321 ctatagattt acttatagct tccttggctg cgaaattacc tgctatactt tctgctttaa
    10381 aattcttaga attaaaatac tctatttctc tatctgtaaa tatcttctcc aaaaacctat
    10441 tatttttgtc tacagccttt cttattctat caatttcaat tatatctacg ccaatatcaa
    10501 atatattcat tgttcctcca gaaaatttat aatacttctt tcaataatta ataatgaatt
    10561 tataaattta ctatcgataa tacacattat tatttagttt atgttactta tcttaattta
    10621 ataatcatta taataaaact gtaactttta aa
//
LOCUS       DAELTA010000127        10646 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.127, whole genome shotgun sequence.
ACCESSION   DAELTA010000127 DAELTA010000000
VERSION     DAELTA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.127"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            249..1028
                     /locus_tag="KPG13_002773"
     CDS             249..1028
                     /locus_tag="KPG13_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG0986473.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1338..2159)
                     /locus_tag="KPG13_002774"
     CDS             complement(1338..2159)
                     /locus_tag="KPG13_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG0986474.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(3169..3987)
                     /locus_tag="KPG13_002775"
     CDS             complement(3169..3987)
                     /locus_tag="KPG13_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0986475.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4155..4736
                     /locus_tag="KPG13_002776"
     CDS             4155..4736
                     /locus_tag="KPG13_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0986476.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4733..4921
                     /locus_tag="KPG13_002777"
     CDS             4733..4921
                     /locus_tag="KPG13_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986477.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(5091..6833)
                     /locus_tag="KPG13_002778"
     CDS             complement(5091..6833)
                     /locus_tag="KPG13_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HBG0986478.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            7262..8638
                     /locus_tag="KPG13_002779"
     CDS             7262..8638
                     /locus_tag="KPG13_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG0986479.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8949..9620
                     /locus_tag="KPG13_002780"
     CDS             8949..9620
                     /locus_tag="KPG13_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG0986480.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9809..10606
                     /locus_tag="KPG13_002781"
     CDS             9809..10606
                     /locus_tag="KPG13_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG0986481.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3946 a         1296 c         1598 g         3806 t
ORIGIN      
        1 tattaacact actttttata atataataaa tactgattct ataatataat taacactact
       61 ttttaaatat aatcaatacc gattctataa tattaacact attttttatg ctatataaca
      121 attattgaaa atgatataaa tataatatct caaataatat gaatataaca tctgaataga
      181 gggtgtttta tttgtaaaaa ataagtttac agtttgagcg tataaactta tggtaaagga
      241 gaataattat gagttataag aagattgcaa ttataacggg tgctaatagt ggttttggta
      301 aagagtttct aaagttatta ataaatgaag aagaaataac agaaatatgg gcagttgcaa
      361 gaaacaaggc gagattaaat caattagtag atgaatttgg aagtaaggta aaaatttttt
      421 caaaagattt atcaaaaata gaagaagtaa aagaaattgg tacattttta agtaaagaaa
      481 atgtatgtat aaaatacctt ataaacaatg ctggttttgc aaaattctgt tcatacaatg
      541 atttaagtat agatgaatct attaatatga ttgatttaaa tattagtgct gttgtagcat
      601 tgggacttat atgtattcca tatatgcaaa agggaagcca tatcatgaat atttcttcac
      661 aagcatcatt ccagccattg ccatatcaaa atatttatag ttcaactaaa gcgtttataa
      721 gaaattatac aagagcatta aatgttgaac ttaagagtaa agggataaat gcaattgctg
      781 tatgtccagg ttggatgagt acaaatctat ttaagagagg tatagttgta gctgaaaaag
      841 ggacaaaaaa tttcccaggc atagtgtctc cagacgttgt tgccaaaaaa gcattgaagg
      901 atgcaaagaa aaataaggat atttctgtgt atggtataaa tacaaaatcc agtcatttat
      961 tagcaaaact attgccacaa aaaatgatga tgaaggtttg gttaatgcaa caacatatta
     1021 aggaataaga gttttggtaa ttaagtatca gttggagatt caatctgtta tatattatga
     1081 gaagttttgt tgagcaaata ataattgtga caaaacggct tatctagtga cgataagccg
     1141 tctataatgt atataaaata tatttttgat tttactttat ttagatattg aagtattaaa
     1201 atatgtagga cttgaaattt tataaagata aatactatga tattttgtaa aaactgcgat
     1261 atgttgatat cacatagttt gactatactg ttaaattgaa gttttcttat tttgcattat
     1321 gcttattact taatgattta gactataatt tccataccat catatgcaaa ataaatgtta
     1381 tcaagtttct tttctaattc actgtaataa ccatatgatt ttccccacat ttcgtcaata
     1441 tgtgtaacaa taattttttt aattctataa tagtttttaa tttcaacaat ctcatctaaa
     1501 gtaaatattt catctttaaa tggtgtgtct ttaagttttg taccatcatt taatatacca
     1561 tcatcagaaa ctagacttat tattaaaata tcagcatcaa agtacatatc actatgagta
     1621 aatggcttgg gattacagca ggcatatatg actttttttt cattctcttt gataacatag
     1681 aaggttatat tcctgtctgg gttattgtta atcaaatcaa tatcaaaatt atttatcttt
     1741 acatgcgaag tttcattaat agaaatgcaa tttataacat caccataata tttaagacaa
     1801 tcgagattcg ttcggtttat atcttcaatt acttcaggaa gcgcataaaa gccaattgga
     1861 ttattttctc ctgtgataaa gtcacatcct attttttcga caattctaat acccttaaca
     1921 tggtctgggt catggtgaga aattgaaata tgttttactt ccgatatttt ttgtctgtta
     1981 caagctactg caatgtcttc aggtgtgtct atcagcattt ttatatcatc aatatataaa
     2041 cttggtccta atcgttcata tcttccgcct ttttgcctgg cttcttcaca tattttgcat
     2101 ttacaaaatg aacttggaat aattgacata ccgccactac caacaatttt taatttcaaa
     2161 gttattacac caccttctaa aaatattaga ttatataaat aatatacaag cacaactgat
     2221 taacaaaatt atgagttaat aaattatatc acatgtaaat atgaaagaat agtatgaaat
     2281 ttttataaac atatattaaa tgtttctcaa tgaaattgac tttgtatatg aactgaatga
     2341 cttattcaat atggataaga catttttctt gtaataagat atgattgtcc tataaggttt
     2401 atgtaatttt gatataattt gatataatat attgattaac aacatacatg ttattggtga
     2461 gcatatttct atagaatgga atatataatt ttttatattt atcattgtat tattgtacaa
     2521 agaaagttaa aaagagtaga gtacaaagga attatttgta ctttactctt ttattgtttt
     2581 gttaaaaaaa ctaatttata ttagtgatta aaatatattt aactagagta taaggatagt
     2641 atataatctt ataattgatt tatctgacaa attaagataa tatcctaatc aaattctagt
     2701 catttaaaca taaaatcata attgatttat ctgacaaatt gagataatat cctaattaaa
     2761 ttgtaggtat ttaaacataa cattataaat aatttatctg acaaattaag ataatatcct
     2821 aatcaaattc tagtcattta aacacaacat cacaattgat ttatctgaca aattaagata
     2881 atatcccaat caaattctag tcatttaaac ataacatcac aagtgattta tctgacaaat
     2941 taagataata tcctgattaa attctagtca ttcaaacata acatcacaag tgatttatct
     3001 gacaaattaa gataatatcc taattaaatt gtagatattt atacataaca ctaaaagtaa
     3061 tttgtttaac aaattaagat aatatcctaa ttaaattgta ggtatttata cataacacta
     3121 aaagtaattt gtctaacaaa ttaaaatatt atccaattaa tataaaaatt acttcttttt
     3181 aactgggaag catacctctg ttacccaatt atcaggatta ctatcgttgc caggacttac
     3241 atgataaata ttaaacatag gaccatccac atcatagtta ttatctgata tccaattacc
     3301 aatagcctca caagcgtctg caacttgatt gaaatttcca ttaacaattg cagttgcagc
     3361 agtcacactt ggaacagtct taaatttaac atgttcagta tctttatact ttccagatac
     3421 agcaacttgt acttcaacat ctacataatc ttctttatat ccagtatcat aaaaaactgc
     3481 tttagaatac tttgggggtt caatttgaac attctggtct tttgtctcta aaaatgcttg
     3541 atgccataac attccttcgt cttgataagt tggtataacc cttctaagtg tcatcatata
     3601 ttttggtgca aaattcttta tagttacatc ataatttttc ataatatcat ttcctccaat
     3661 tcttttaata gtggtttcta tttttaatag ttttttctgt gtatcttcta attgttcttt
     3721 tacttgagaa tggtggatat ttagatattt tatgagactt tctttatcgt tatattctgt
     3781 caaaatttct tttatagaat acaagccaaa gcccatatct tttagtgcat ggattctgtt
     3841 tgtagtagaa agttggtctg cactatagta tctatatcca ttagttttat tcgtatgaga
     3901 aggagttaaa agtcctattt catcataatg acgtaacata cgaatactaa ttttacttag
     3961 ctttgaaaaa tcgcctatct taaacatgtt tttgtctcct ttataaaggt acttttagct
     4021 taatattaat ataaaccata tcataatgtt agagtcaact aaaattataa ttaaattata
     4081 ttgacatatg atgaaaaaaa tattatactg aacatgttca gaactgaaaa tgttaattgt
     4141 tttggaggtc tattatgaaa agtaatgata aagatgctag agaacgtata attgaagtta
     4201 cattgaatct tttaaatgaa gtagatgata ttgaggagat tacagttaga aaaattgctg
     4261 agcgtgcaaa tgttggagtt ggtttaataa attatcattt taaaacaaag gataatttgt
     4321 tgagtactgc cataggagat gtgatgtcaa atataatagc agagctatat gacgatagtg
     4381 tatatactct aagacctatt gaagatttaa aaaatttatt aaaaaaattg tgtgatactg
     4441 gattacatta tgaaaaagtg cttccttttg tattgaatca atgtataacc aatggagata
     4501 tgcaagcaga acttgatatt gtccctatgc ttcgtaaaat ttttggaaat aaaaaagatg
     4561 aaatgagtct aagaattatt gccctgcaaa ttattttacc aatacagatt tctgctttat
     4621 ctacagaatc atttcagtta tacagtggta taaatataaa gaataaatat gagcgagata
     4681 aattcattga tattttaata gaaaatatta ttggagagga tgttgatgta agatgaaaat
     4741 tataactatt ggttcaagtc ttatcactgt tttacttttt ctttcaacta tgatttgtgg
     4801 gttctggatt aagaataata aggtaacaga tgcaagttct attaagttcc atatgaatag
     4861 tgctattttt acagggatat ttttattgat ttccacaatc cttttaatta tatatatata
     4921 aaaataataa tattattaat aaataaagta tcagaaatta taaagacaag agtcttaaaa
     4981 atttctgata ctttaccata taattttatt aaacattaat tgtgtgtgaa aaaatatcac
     5041 ttatttcaat ctaatactaa atcttaagag tgagtataaa gtattagaaa ttaagaagca
     5101 ttttttagtt caagcggatt atctatacca aaataatcat ataaagtaga gcttatttcc
     5161 tctaaatttc catcttggaa aggtaattgt aaatttacct tttctaccac atctttaaat
     5221 ccagcatcag tattttgatt taaaaaatcc atatatacat caacagcttt tctgtaatct
     5281 tcttgtgata attgccatat ctgaagtgat acatcagatg caagaccata actcaagtaa
     5341 taaaaaggaa cttggaagtt atgagataca agtatccaaa aaggagcatt tttttctcta
     5401 agtatattat tggaaactcc atattcttcc tctaaatcaa aaaacaattt attgatatca
     5461 tttagactca tatatggatt cttgtatata atttcttgaa attcatcata aagacaagca
     5521 tcaataatag tatttaaaac aatcgataag tgttcttttt ttatagcttc agattgttta
     5581 ccaaaaaaat catcaaaata tttataaaat agaatttcta aagatgttga atgaacttca
     5641 catacatcta tacttggttg catcttattg tttaagttaa tactgttgta gtaattataa
     5701 aaatgaccaa actcatgagc aaaactagta acacctaaaa aagaattatc ccatgtatta
     5761 aacaagaaag gtgctttata tttatttata taagtagtat atcctccagg tgatttgttt
     5821 tttccagaag aataatcgta caagtcatat tttttcatat agtcgaagct ctcttttagt
     5881 tttggagata tatcttcaag tactttatca aacttcctaa atgagcgatt tttatatact
     5941 ttaatataaa tagaagaatc agatggttta gatgaaatct ctctatataa tggaactatg
     6001 taatctttta catcttgtct aaatttttta gcttcttctt gagaatagtc tttgtttagg
     6061 ttcatatagg cataatctat ataattctta aatccttgct tacttgctat ttcagttctt
     6121 gtctgtataa gttctaagaa tatgttacca aatataggat tgtatttttt taagtaatct
     6181 gaataaattt tcacatattc ttcagggcta agatttggtc ttgacaaggc ttcttcaaag
     6241 tctacctcta taccatctat tgatactgta gatttagcta ataagtcttc atattttaca
     6301 actaggtctt gctctttttt aagcaaacct tcaacttctt tagaattaag ttttccaatg
     6361 ttctttagat aattaaatgt atcctttcca agttcttttt ctaattcttt agagaattta
     6421 gaagttacaa atctatcttg aaagtctttg tatgacatgt caacatctac tgatttactc
     6481 ataagatact tatattcctt agagtaaaat tcgtctgatg aatctatgtt atttctaatt
     6541 tttgctatag taagtgtact gtaaaaatct tgataaattt tatcaacttc attaaataaa
     6601 tttaattgtt ccttagctgt tttagctgtt aaaagtttgt cgttgcactt gttaatgttt
     6661 tcgcaaattg actgtatatt aggacgctta tattccattt tttcaaaagg aatagttcct
     6721 ctaacagaat ctttttttgt atcattagta taatttctta tgtatgatgc aaatataata
     6781 aaaactgcaa ttaaaattag aaaaacaaat attttcttct tggatttaac cataaaaatc
     6841 ctccaatcat ggatatagtt attaattatt ttactatatt aaatataaaa aatgcatgtt
     6901 aagaaaattt aacataaaga tatagataag attataaata atataaaaaa tttaacttca
     6961 aaaaagtata tgaaatttat actggtaaaa tgataaataa attttaagta caaaatagta
     7021 aaagctatga tagttttaga cctattatat gtaaaaaata tagatgtttt tggtgcaatt
     7081 taaattaaaa gatatatata aaaatttaaa atattccaaa cagaaattat aaaaattact
     7141 atcattgtct ttatacaata tttatactat aatagaataa taatttagag gaaagctcta
     7201 aaaataaaca aattaagggt cttaacagta gtgattttat gttaaggagg tataaagaaa
     7261 gttgaaaaca atagttagaa gacttattct ctacgcagat ggaatgcgtc ctactcaaat
     7321 aatggtatct gggtttgctg cgattattgt aatcggagca ttgttgttaa cactcccgat
     7381 agcgtcgcag agtggagaga gtataggctt attaaatgct ctatttacgg ctacttcagc
     7441 ggtttgtgtt actggtctag ttatggtaga tacagcaact tattggagtt tatttggaca
     7501 gatagttata ataacattga tacaaattgg tggtcttgga tttatgaccg tagccacaat
     7561 gttttcactt atggcacgaa aaaaaataca actaagagaa agattattaa tacaagagtc
     7621 attaaatcaa gctgatttat cgggacttgt aagacttaca agatttgtat taataataac
     7681 cataacaata gagggaatag gtgcactggt tttatcaact gtctttattc cacaatttgg
     7741 tttgtctaaa gggatatggt atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg
     7801 atttgacttg atgggcagtg taagtggccc atttacatcg ttaaattctt atgtaaacaa
     7861 ctttacagta tcaatgactg tttgcgcact tatagtgctt ggagggctag gattccctgt
     7921 tgtacttgat attgtaagaa aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt
     7981 attgttttca actgctacac ttatttttgt gggtgcatta tttatttttc ttatagaatt
     8041 taatcaaaaa gctactatgg cggacttgcc tttaaaggga aaagtactat cagcaatatt
     8101 tcaatcagta acagcaagaa ctgctggttt taatacgctt gatttagcta ctttacgaga
     8161 aagtagtgta tttgtaatga taattttgat gtttattgga gcatcacctg catcaactgg
     8221 tgggggtata aaaacaacaa cactagctgt tttaatcatt acagttagaa gttttctatc
     8281 aggaaaatca gatatagaag cttttgaaag aagattagct ccatcaacta taaaaaaatc
     8341 tcttggtata tttgtaatca gtatatcagc agttattttt ggaacattga ttatttcaat
     8401 aactcagcct aattttacat tagttcaatc agcttttgag gttacatcag cacttgctac
     8461 agttggttca agtttagctg gaactccaaa cttaaatgct ttaggtaaga ttataataat
     8521 aatctttatg tttatgggta gagttggttc tcttacttta tttatggcta tactttctgg
     8581 tggtagaaga aagagtcaac caatcagata tgcagaaggt aaaattatgg ttggttaagt
     8641 taccaaggag tagtataaat ttataatggg tttagagtag cactgtaaat ctgttgataa
     8701 aataaatgag cctagatgta attatatgaa gaaataaaat caactaatga acaaaattaa
     8761 ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa aaggtaaatc aaaattaaat
     8821 taatctgtgt gtataaaatt atatattgtg tataagtttt gtatgtgtaa agtatataag
     8881 ttttgtatgt gtaaacaatt aatttaagag atattatata aattgtgtat tagggaggaa
     8941 gtttcgagat gaagcaatat atagtcattg ggtgtgggag atttggaagt tcagttgcgt
     9001 ctactatgca tcttttagga catcaagtaa tggcaataga caaaaatgaa gattcagttc
     9061 aaagtatatc tgacaaggta acccattcac ttatagtgga tgttactgat gagcaagcgt
     9121 taaggtcatt aggtttaggt aactttgatg tagcagtagt tgcaataggt tctgatataa
     9181 gggcatctat aatggcgact cttatagcca aagaaatggg tgtagagttg ataatatgta
     9241 aggcaaagga tgaattacaa gctaaagttc tttataaaat tggtgcagat agagttgtat
     9301 ttccagaaag agatatggga gtaagagttg cacacaattt agtttcggat aatatattag
     9361 accatattga acttgaccca gagtattcaa ttgttgaaat cgtaactcca aatagttggg
     9421 ttggcaagac acttatagag cttgaattaa gagctagata tgagataact gtacttgcta
     9481 taaaaacagg taaaaatata aatgttacac cttctccaga tgaggaactt acagccggaa
     9541 gtatcctagt tataatcggt caaaatacta gtataacagc gataacatct ggaaataagg
     9601 ggataattag aagaagataa tttactattt aatatatatt taattgcaat gaaagcagat
     9661 agtatcatat aattatgaat agttatatga tactattttt ttaatcgaag gtagtattta
     9721 tttgtaagat tagataaaga gaagttaaat taaaagtaag gaggctgtgc taatataaaa
     9781 atttataagt tattagcatt tagataaaat gattacaaat ataaacagta aggataatga
     9841 aaagttaaag tatacaagag cactattaaa atcaaagaat aggaataaag agtcaaagtt
     9901 cataatagaa ggatacagaa tagtaatgct tgcacttgaa tgtatggcaa accttgatta
     9961 tgtatttatc aatgaagaat ttgaaaataa gaaagaacat gtaaaactat tagaagattt
    10021 ggataaaaaa aacacaaaga tatacaagac tactaataaa aactttaaag aattagtgga
    10081 tacagaaaat actcaaggaa taataggtgt agtttcattt aagaaaaaaa aattaagtga
    10141 aagtataaat aaaaaagata aatttgtatt gattttagac agaatacaag acccaggaaa
    10201 tatggggact ataataagga ctgctgattc tgctggagta gatgccataa tagcactaaa
    10261 gggatgtgtc gatatataca acccaaaagt aattaggtct actatgggtt ctatttttga
    10321 tatgaatata attgatgctt cacaagatga aactgtggac atgcttaaat cattggattt
    10381 taatatagtt tcaagttact taaatacaga aaatttttat gacaaaatag attatggctc
    10441 aaaagtagca ttggtgatag gaaacgaagc aaatggaata aatgaagaac ttgtatcaaa
    10501 gtctgatatt ttggttaaga tacctatata tggtaaagcc gagtcgttaa atgctgcgat
    10561 aagctctgct atactgatgt atgaaataaa aaaatactta atttaatgta ttgtaataaa
    10621 tataatgtca tgttataatc ttgaat
//
LOCUS       DAELTA010000128        10633 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.128, whole genome shotgun sequence.
ACCESSION   DAELTA010000128 DAELTA010000000
VERSION     DAELTA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.128"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..1268
                     /locus_tag="KPG13_002782"
     CDS             132..1268
                     /locus_tag="KPG13_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986482.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            1261..2808
                     /locus_tag="KPG13_002783"
     CDS             1261..2808
                     /locus_tag="KPG13_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG0986483.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            3143..4009
                     /locus_tag="KPG13_002784"
     CDS             3143..4009
                     /locus_tag="KPG13_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG0986484.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            4150..5292
                     /locus_tag="KPG13_002785"
     CDS             4150..5292
                     /locus_tag="KPG13_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG0986485.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            5676..6914
                     /locus_tag="KPG13_002786"
     CDS             5676..6914
                     /locus_tag="KPG13_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986486.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            6943..7830
                     /locus_tag="KPG13_002787"
     CDS             6943..7830
                     /locus_tag="KPG13_002787"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG0986487.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            7864..8493
                     /locus_tag="KPG13_002788"
     CDS             7864..8493
                     /locus_tag="KPG13_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0986488.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            8498..8962
                     /locus_tag="KPG13_002789"
     CDS             8498..8962
                     /locus_tag="KPG13_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG0986489.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            9212..10579
                     /locus_tag="KPG13_002790"
     CDS             9212..10579
                     /locus_tag="KPG13_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0986490.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         3963 a         1126 c         1810 g         3734 t
ORIGIN      
        1 tagcaatatt cagacaaata gtgacctaaa aatcgtactt tgtttacata aataaaatag
       61 tatgatataa ttataaaaac aacttataat attggagaag gttgcttgtt cgaaaaaggg
      121 gatgataaat tatgatagag ataagaaatg taacaaaaaa aataggaaat aatgttatat
      181 tagatgatat ttctctagtt gtagaaacag gtacattggt agttttgatt ggttcaagtg
      241 gttgtggaaa gactactact ttgaagctta taaacaaatt aataaaacct acatctggtg
      301 agatatatat taatggcaaa ccaatttctc aggaaaatga aatagagctt agaagaaaaa
      361 taggatatgt tatacagaac acaggcctat tccctcatct tacaataaaa gaaaatatag
      421 agctaattcc acgcctaaaa aaggaaaaat ccgttgaaga aattgaaaaa agaacgttgc
      481 aactacttga gatggtagga cttgatagtg atgaattttt gaataagtat ccttcagaac
      541 ttagtggtgg acagcaacaa agaatcggtg ttgcaagagc cattgctact gatgctgaaa
      601 taatattgat ggatgagcct tttagtgctt tagacccaat aacgagaaca tctttacaag
      661 aacaattatt ttctcttcaa gatgaattga aaaaaactat aatatttgta acgcatgata
      721 tggatgaggc tttaaagata gctgataaaa tttgtattat gaaggatgga agaatagcac
      781 aatatgatac tcccgaaaat atacttagaa agcctgcaaa tgactttgta aaagatttta
      841 tcggagaaga tagagtttgg gataatccag aatatataaa agcaagagat attatgataa
      901 aaaatcctat tgctgttaat tctactagaa ctgtaactca aggaatagaa attatgagaa
      961 cgagtaaggt tgatagctta ctaattatag atagagcaaa aacattaaaa ggtattgtta
     1021 ctgtaaaaga tatgaaagat attgatgata agtcaatatt acttgctgat attatgtcaa
     1081 gtgagccttt gcatgtaaat gaaggggata atttagtaga aatactaaat gtaatgaatc
     1141 gtaattcggt tggatatatt ccggttattt ctgatgaaaa taaactggta gggcttatta
     1201 ctagaagtag tttactttct gttttaagtg aacaattctt agaaatggag gtgagtgttc
     1261 ttggctaatt tttttaactt tatactatta caaaaagata agattataga gcttttaata
     1321 caacatatga gtctaactgt tacatctatt ttaatagcta taatagttgg tgttcctctt
     1381 ggaataatta tttcaagaat ctcatctctt agaaagtttg tacttggatt tgtaaactta
     1441 gtccaagcag ttccgtctat ggcacttttg ggtcttcttg tacctatact tggtatagga
     1501 agtaaaccag ccatatttat ggtagtggtt tattcattac taccaattgt aaaaaatacg
     1561 tatataggta taacaagtat tgacccagta gtattagaat cagctaaagg tattggtctt
     1621 acaagaaatc agacattatt taaaatacaa ttccctttag cacttcctat aataatgggt
     1681 ggagttagaa tatctgcagt tacagctgtt ggtttgatga ctcttgcagc atttataggt
     1741 gctggtggtt taggatattt agtattttca ggggttcaaa ctgtaaataa taatatgatt
     1801 ttagcagggg caatcccatc atgtataatt gcactgttag ttgattattt atttagtaaa
     1861 attgaagttg cagttactcc aaaaggatta aatccaaaag cacctaagaa gaattatata
     1921 gcacttaaag taatatctgt agtggttgtt gtttcaatgt tgttcgttgt ttttagttct
     1981 agtttttcaa gtaaaaaaga tacaataact attggttcta aagattatac agaacaactt
     2041 atacttggta atgtttatgc tgaattagta gaaaaaaata cgaatcttaa agttaagaag
     2101 aatttaaatc taggtggttc atctgttgcg tttaatgcaa ttaaatctgg tgagttagac
     2161 atgtatgttg actatacagg tacacttttg gtaaatgtaa tgaaacatgc tccgattaaa
     2221 gatgctgatg aagcatataa tgttgtaaaa gatactatgg aaaaagaaaa tcagttaact
     2281 ttacttgacc ctcttggatt taataatact tacacattag ctatgatgcc tgaaactgct
     2341 gaaaagtatg gaataaatac tatttctgat ttgactaaat atggtaaaga atttacattc
     2401 tcaccaactt tggagtttga aaatagagaa gatggtcttg tagggctttc tagagattat
     2461 ggtttaaaat tcaaagatgt aaaagcaatg aatggaagtt taagatatac agctttagat
     2521 aataatgaat ctcaggttat agatgcattt ttaactgatg gtcttattaa aaagtttaat
     2581 cttaagatat tagaggacga taaaaatttc tttgtaccat attatgcagc accattagta
     2641 agagaagata ctttgaagaa atatcctgag ttagaaaaag ttatgaatat gctttcagga
     2701 aaaataaatg aagaaactat gagagaactt aactatcaag tggatgaact tggtaaatca
     2761 ccagaagaag ttgctcattc attcttagtt aaagaaggct tagtataaaa aattaatagt
     2821 ttatatcaaa aaatttaaga gagtgtctca aaatgaactt tttagttcat gaggcactct
     2881 tttttatatg aaatcaaata tattttatca tttcaacaat aaaaaagaag attatcttta
     2941 ttttgagaca gtctcttatt tttatgtata ccaaaaccat acctacgatt tttatttata
     3001 attaagtttc aaatagaatg aacaagtaca accaattttg aatataaata tagtaaatgt
     3061 gcttatatac gattttattg tctggttgat tgatattgat agatagcttt ctttattgaa
     3121 tcgaagggag ggggaatttg ttatggataa ctataagaaa gtgaagcaag tattgtggat
     3181 aattttattt gctaattttg cagtagcatt attaaaaatc attattggta atcaaataaa
     3241 aagctatagt atgacagcag acggatttca ttctttgtca gatggagcat ctaatatagt
     3301 ggggcttata ggtattttct ttgcatctaa accaaaagat aaaaatcatc cttatggaca
     3361 taaaaagttt gaaattataa ccagtttatt tataagtggt atgttgtttg ttatagcaat
     3421 taagataatt cttagtgcag tgttaagaat agctaatcca gttgtacctg ctataactat
     3481 agaaagttta attgctttaa ttattacact ttttataaat atttttgtat gcatgtatga
     3541 atatagaatt gggactaaac taaatagtta tgttttgatt tcggattcat tgcatacaag
     3601 aagtgatata tttgtgtctc taggtgtatt ggttacttta gttggtgtta agttaggatt
     3661 tccagtaata atagaatcta tagttcctat tattatttct gcattcataa tttattcggc
     3721 ttatggaata tttaggccat ctattggaat tttggtcgat agagtggctg tagatgagga
     3781 ttatataaaa gaaattgtgt ttgaatttaa tgaggtaagg gatgttcaca atatcagaag
     3841 tagaggtagc aaaagttcta tttatattga catgcatgta atggtagacc catttataag
     3901 cgtagagcaa tctcatgatt taacacataa aattgagaaa caaatacaag aagaaattaa
     3961 tgaaaatgct caagtaatag tccatattga acctttttat agtttttaac ttagctataa
     4021 aatacaagtt ataaaaaaat ataatttata aaaaaaatat tataccataa ataatttata
     4081 aaaattattt atggtataat attttctata aagaaattaa gtcttaatat atctaaggag
     4141 gaatttacaa tgagatacgt tgaatatgga aagacaggga aaatggtttc agtagttggc
     4201 tatggaggat taaggtttga tttagaaaaa agtaatcaag aaaatgctga tttagtcaaa
     4261 tatgcgtatg aaaaaggtat taattacttt gatacagcac cagggtattg tgatgacaga
     4321 agtgaagata tatttggact agcctttaga caaatgataa aggaaggtaa gaccgatttt
     4381 tatgtatcaa ctaaagaaaa gccaaaagca tgtgatacat cagaaaaagc tatagaggct
     4441 gtaaaaaaat caatagaacg attaggcgta tctaaaataa atttttatca tgtttggaat
     4501 attagaaaga tggaacatta cgaactagca atgagacctg gaggtcaata tgaaggatta
     4561 ttgaaatgta aagaagaagg attgatagac catatagtat tttcatctca tcaaccagga
     4621 gatgaggtta tagatattct aagtgaaaat aagtttgaag gtgtaactat gggaataaat
     4681 atattaaact ttccatatag gctaaaaggt gccaaatatg ctgttgataa tggatatggt
     4741 gttgtggcaa tgaatccatt aagtggtgga actattccaa agtataataa agaactttct
     4801 tttctatcaa aagatggcga aagtactata gaatcagctt taagattcaa cataggaatt
     4861 tcacaaataa gtatatcttt gatagggttt aataaaaaac aagatataga tgatgcatgt
     4921 aaaattgcag acgaaaatag aatttattca gatgaggata tacatgatat tgagactaga
     4981 ttaaataaga atatgaacga aatatgtaca ggttgtggtt attgtaaagt atgtccaaaa
     5041 ggtattaata ccccagccta tatgcttttt tataatgaaa agcagatgtt taaaaagagt
     5101 gatgaagaaa tgacaaagtt agtttatggc ttaggacatt ggaattacac aatgaatagt
     5161 aaggcaaaag ctaaagaatg tatatcttgt ggaaaatgtg aagttgaatg tactcagcat
     5221 ttacctataa tagatagatt aaaagaaatt aaaaaatggg aagaagatgg agctaatagt
     5281 gttaaagtct aattaatttt atactatatt tgaaatttgt atagtattta gtaatgtttt
     5341 aaaatatagt tttaatattt aaggagacag attataattt tgtctcctta aattatgctt
     5401 taatttgtta tagtttaatt ccataagtaa cattgtatat ttttatataa agtttttcaa
     5461 atattttaca ttaatattgc ttacaaattc tcattttata caacactaat ctatgttata
     5521 taaacaatac ttgactgatt ataacatcaa taaagtaaat attgtggttg taaataaaga
     5581 gttattcaat tacaataaaa caagtaagaa cattatgaag atggctatta aatataagga
     5641 ataaattgta ggtgtcagca aatcctatat aatgaatgga tggtgatata ttgatgagta
     5701 aaatattaaa atataaaaac gagatgtttt tgtttatatt agttgtaata acttacttaa
     5761 taataactaa gatatttttt tctaagacta ctatatttta tgatttaaac aatacttatg
     5821 atgtattgtt agatacagat acaggagttt tattcaattt aaatgtattt gctataagtc
     5881 aagacaatag taagcatata ttgttttcag caattatatc aatttttgca tatccaatat
     5941 acctattttg tacttcaata gcaaatcctg ggacaacaga ttttaatagt gcctatgggt
     6001 ttggactaat ttgtctacaa ataataacta gtgctatgtc aataacttta gtctttaatc
     6061 atattaaaaa gataaaaatg cagagattaa ctttaatttt acttactatg ataatgatat
     6121 tttcttttcc acagttattt atgactttaa atgtagagag atttatatat agccaatttt
     6181 cattaatatt tttcatagtt attgcaaata agatgaaagg aaaaaattct tatttggtag
     6241 aattagcagc tattcctcta tttggaataa ctattagtaa tatatatttg tattttttta
     6301 atatgatatt tgagtttaaa ttaaaaatag gaaaaatgtt aaaacattta attacattta
     6361 tcctaatggc atatatttgt gtagtttcaa cgaagtctta tgagagtttt atgaaccttg
     6421 gaaatgtaat acagtatgat acgaaattta taagtggaga accaattttg gaaaagattg
     6481 caatgattat agagagatta ttgtattcgg ttttttattt tcctggagca agtattaaaa
     6541 aagggttatt tttgcaaaat ggagaagttg ctacaatacc agtaattctt acattattag
     6601 ctttatgttt ttgtgtttta tcagttattg aaaattcaga aaaaagagtt cctaagctat
     6661 gtatgggaat aattatattc aatctaactt tacatggtat agtaggatat aatctagtca
     6721 attcaagtat tatggctata aattttagtt ttgcagttat aattttattg gcttacttta
     6781 cgaaagccct tagaaaaaat gaaaagaata tgtataatat atttctatcg ctgttattgg
     6841 ttactatagt aatcagtaat gtcaatggat ttatagaaat tttaaacata gggataaaat
     6901 catatcctgt ttaaaaaata atattgaaag ggtagtaagt ttatgaaaca gaccaataca
     6961 agtataaggc ttcttactaa aatttcagat tatataaagt taatgagaat aaagcaatat
     7021 ataaagaatt tctttgtatt ttcagctata attttttcaa acaacatact aaatataaat
     7081 ttatttttaa atacgtttat tgcgtttgtg tgtttttgcc taatgtcatc atcagtatat
     7141 gcactgaatg atgctataga catggataaa gataaaaaac atcctaaaaa atgtaataga
     7201 ccagtggcaa gtggaagaat aagcaaaaaa agtgctaata tactatttgt agtattagcg
     7261 ttaatttcag tctgtttatc aacagttgtt agttttaatc tttctataat tctcagtctc
     7321 tatttgataa acaatatatt atattctctt aaactaaaga atataatact attagatgtg
     7381 ttttcaatat ctcttggttt tatacttagg gtttatgcag gatgtgtagc cataagtgtt
     7441 agtttatcaa attggataat tttatgtaca tttttcttat ctctgtactt agctcttgga
     7501 aaacgtaaaa aagaaataga aactctaaga gatgatgcag ttgaacatag aaaaatattg
     7561 gaagattatg acatagaaaa tctaaatcaa atgatgatag ttatattaag ttctacaatt
     7621 gtatgttatg cattgtattc tacaagtaat ccagaaaagc cacatatgat atttacaaca
     7681 atatttgtag tttatggtgt acttagatat aattatataa ttaacactac aaatgaaaat
     7741 aatcctactg atattgtcct aaaagataat gctttaaaaa taaacgtaat cttatggata
     7801 attacatgtt taataatatt gattttttag tgatattttt agtgatagga tggggaaata
     7861 cttatgaaaa aaagtgtatt attacttgat gtagattata ctgttataaa tactgatagc
     7921 atgatagatt tttttattta ctctttaaaa aataaaacct ttaaaactat tataaagttg
     7981 ccatacatta tttttattct atttatgtat ttaattagaa tgataccctt gaagaaggca
     8041 aaagaggcaa tcttttatcc aatagtagat tttagtgaag aagatttaaa aaagtttttt
     8101 gatgattgca taatgaaaaa aattaacgaa tctatgaaaa aggttattta caaaaataaa
     8161 gaagaagata atgttataat tatgataact gcatctccat atgcatatat gaaatatttt
     8221 aagtattatg gtcttgcaga cgaggttata ggcacggagt ttttttatga aaattcaaga
     8281 tataaaaatc aattcattgg agaaaactgt aagggaattg aaaaagtaaa aagaataaag
     8341 gctgtattag gtaaacttgg gatagagatt gattatgaaa attcatatgc atattcagac
     8401 tctaagagtg atttaccaat gctttcattg actaaaaatg catttttagt tagtaaaaag
     8461 gatggtgagg ttatagagca aataaattca taacttaatg tcacttagaa taggagggaa
     8521 tagtttggga tatttacaca tattaatttt atctatggtt cctgtaacag aattgagggg
     8581 agccattcct ataggtattg caatgggctt aaatcctata tgggtttatg tatttagtgt
     8641 aataggttct acattggtat cgattccctt gatattgaca ttcagacatg tattacaatt
     8701 tttaagaggg aagaaactat ttaaagggat tgctgatgta attgatagaa aaataaactc
     8761 aagaatgaag aaattaaaaa gtgtttcgat aattggtata atactatttg taggtatacc
     8821 actgccaact acaggaacat ggactgcatc tgctatagct tccatattaa aaatgagaat
     8881 aaaagatgct tttatgggag tatttcttgg caatttgtta tcaggggtta tagtttcagc
     8941 tctttcttta catataatat aaaactatct aagtaatata cttggatagt ttttttatga
     9001 gtatatgtga aaaattttta ataataaata aaaaatgaga taaagtattt aacttcagtt
     9061 agttctaaaa tgtttactat ggcaaaagaa atatagtata gtattacttg tagggaaagt
     9121 actctaaatt tgtctgctaa taaaatgaaa tttttatata gaataaaatt ttagtgatac
     9181 attttaatta ttggattgag gaggttttaa aatggaaaat tcaaacacta acttaggtag
     9241 tgaaagtgtg ggtaaattat tatttaaact tgctacacct gctattatcg cacaaattgt
     9301 aaatgtttta tacaacatag tcgataggat ttttattggt agaatggaaa atggagaagt
     9361 tgctatggct ggtgttggag tggcatttcc aataataatt ataataactg catgtagtta
     9421 tttaatcggt atgggaggag gtccattagc tgctataaaa atgggtgagc aaaacaatga
     9481 tgaggcagag aaaataatga gtaacagttt ttctgtattg gtgattttag caatactttt
     9541 gactataggt tttaagattg gaaaagagcc tttactttgg atgtttggag caagtgaatc
     9601 tacaattgga tattcaatgg actatttaaa tatttattta ataggtacag tgtttgttca
     9661 gatttcaatg ggtatgaata cttttataaa tacccaaggg tttgctacaa ctggaatgat
     9721 gacagttgct ataggtgctt taataaacat aatacttgac ccgattttta tatttggctt
     9781 taatatgggt gtaaaaggag ctgcattagc tactataatt gcacaaggcg tttctgctat
     9841 ttgggtactt atgtttctct ttggaaagaa aagtatactt aaaataaaaa agaagtatat
     9901 gataccaaaa gcaagtatca tcttaccagt gcttggcttg ggaatctctc catttataat
     9961 gcaatctaca gaaagtttag tacttatagc cttaaatagt aaattacaaa tgtatggagg
    10021 agatttagca gtaggttcaa tggccataat gagcagtata atgcaaattc ttatgcttcc
    10081 taatatgggg gttacacaag gagcacaacc aataataagt tataattatg gttcaggtca
    10141 acttgataga gtaaaaaaga catttaagtt gtgtttattg tcatgtttta catattctac
    10201 tattttatgg cttttattaa tgatatttcc tgcatttttt gttagtatat ttaataagaa
    10261 tccgcagtta cttagtatga ctagttggag tataaaaata tattttgctg gtgcttttat
    10321 gtttgggatt caaatagctt gtcaacagac atttttagca cttggaaaag caacaatatc
    10381 tttggtttta gctctactta gaaagattgt acttttaata cctttaatat ttatacttcc
    10441 aacattcttt aatgaaaaac tttttgcagt aattttagca gagccagttg cagatataac
    10501 agccgctact ataacagcaa tttctttctt tatattttat aaatgttttc tatcaaaacc
    10561 taaagcaata aaagaataag atatacaata taaatttttt atgaaaatat tttattagat
    10621 aaaaattaga att
//
LOCUS       DAELTA010000129        10580 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.129, whole genome shotgun sequence.
ACCESSION   DAELTA010000129 DAELTA010000000
VERSION     DAELTA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.129"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            48..272
                     /locus_tag="KPG13_002791"
     CDS             48..272
                     /locus_tag="KPG13_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986491.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            325..849
                     /locus_tag="KPG13_002792"
     CDS             325..849
                     /locus_tag="KPG13_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0986492.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(954..1100)
                     /locus_tag="KPG13_002793"
     CDS             complement(954..1100)
                     /locus_tag="KPG13_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986493.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(1239..2165)
                     /locus_tag="KPG13_002794"
     CDS             complement(1239..2165)
                     /locus_tag="KPG13_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0986494.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            2355..4226
                     /locus_tag="KPG13_002795"
     CDS             2355..4226
                     /locus_tag="KPG13_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG0986495.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(4318..5253)
                     /locus_tag="KPG13_002796"
     CDS             complement(4318..5253)
                     /locus_tag="KPG13_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG0986496.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            5773..6285
                     /locus_tag="KPG13_002797"
     CDS             5773..6285
                     /locus_tag="KPG13_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG0986497.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(6440..7501)
                     /locus_tag="KPG13_002798"
     CDS             complement(6440..7501)
                     /locus_tag="KPG13_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG0986498.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(7625..8518)
                     /locus_tag="KPG13_002799"
     CDS             complement(7625..8518)
                     /locus_tag="KPG13_002799"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG0986499.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(8528..10009)
                     /locus_tag="KPG13_002800"
     CDS             complement(8528..10009)
                     /locus_tag="KPG13_002800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG0986500.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(10117..10348)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         3995 a         1359 c         1508 g         3718 t
ORIGIN      
        1 aactaataat aagaaaaata tcaattatat aaggagtaga taaaaatatg ataataaaac
       61 ttactaaaga caattttaat tttagattta aacaatatga aggtatgttt tttcatgctg
      121 aagtagttga aggtatggaa tccattagat ataaaataac taaggataaa gaaggtacag
      181 aattaaaaga atttggaagt acacatataa aagataatat gatatatgta cctcaggtag
      241 aagcatacat agatttgaac agtataaaat aaagatgtaa aattttaatt aaaaataaag
      301 attatgtttg agggggaatc caatatgagt aagcctatag atataacaat aagacctata
      361 agaagagaag atgcacaagc ttttaatgaa attagaagaa catacagtgt tatgagaaat
      421 actttggcat taatgagtga cagagtcgat aaaactgaag ctatgttagc ttctttggga
      481 gaaaatgatt atatgtttgt agctgaaact aataataatg gagaaaaaga tgtaataggt
      541 tttgctgggt tacatgtaaa ttcttctcca cgattgagac atagcgcaga aataggaata
      601 agtgttgcag agaattatca gggaagagga gtaggtaaga agcttatgga acaactttta
      661 gatatagcag ataattggat tatgttaact agagtaggat tagaagtaat tgtggataat
      721 gaaaagggat taaatttata taaaaagcta ggttttgaaa tagagggaac taaaaaatat
      781 gctgtaatca gagatggaaa atttgaagat gtttatatta tgggaagata taataaaaat
      841 ttaatttaaa gaagctaata gagaagtaaa atagagtgtc tttaataaaa aatttacatt
      901 aaagataatc tattttactt ctctattgtg ttaaaaaact tatttttaaa tgtttacaaa
      961 aacattctat atacaaataa aattattagc aatatcaacg ctattttaaa agctaaacta
     1021 gcaacagttt taatgatttt tacagaaaca cctatagcaa ctatagcaaa tactatttta
     1081 aagagtaaat ccatatccat atttaagcct cctattctat aatacatata gatattattg
     1141 acaaatatct aggaaaaaat tccatcaata tactacaata aaatgtttat atttgtaatt
     1201 tattgaatat gttattatat aatacataaa aaaataaatc aagataagag taaaatactt
     1261 ataaaaggta tagatataac tgaaaatagt gttgtaaaaa acacacattg tgaagctagt
     1321 gttatattgg agtcatattg attagccatt atagcagtat ttgcagcagc aggcattgaa
     1381 gaaattacta ctggtatagc taaaatcatc ttatcattta tccatccttt tagaataaaa
     1441 tacacaatca caggaagtac caataatctt ataaaagtga ctatatataa ttttttgttg
     1501 acaaaacaat cgagagctga agaattagca agtaagcttc caatgataat catggatatt
     1561 ggtgtagtta tacttcccaa catctcaagt ggctcgttta taaattgggg taacctaagt
     1621 cctgttacaa ataaaaaaag tccaataaca atagctattg tagcagggct tataagtgat
     1681 tttattgaaa aacctctact tgtactgcct ttagataaaa gataaattcc taaagtgaaa
     1741 gaaaataagt taaaaggcaa attgaatatt gcagtataga atattgcttc atgacctaaa
     1801 actgcttcaa ttacaggata acccataaaa gcaacatttg aaaaaacaac tatatactgg
     1861 tatattccca aatctttttt atcattacat tttaaaatgt atttcaataa aaatgcaatt
     1921 ataattgaaa ctatatacat aatcaaagat gtaaataata acaatagtat tttttgtatc
     1981 attttactat caaagttaat ttgcatggag ctaattatca ttgatggtaa aaaaactgtc
     2041 attgtcaaag ttgaaagctt agatgtgcag tggtcatcta ataagttgaa ttttctgaca
     2101 aaatatccaa ctaaaataat tataaataaa actgcaactt ggataaaaat attgctgaaa
     2161 ttcataaaat gtccccccta tatatacatt atataccaat tacataataa aatctaatga
     2221 ttataaaatt taaaatttaa tgttacaata tagtgacaaa aggtaaaaat tcaatattat
     2281 agcattaatt gtagattata atgtataata atgtttgaga gaatatattt tataaaatgg
     2341 gtaggtagcg aataatgaaa aatacaaaat tttctataaa aaaaataaaa aatatatcta
     2401 atgacccgaa agtcgtacat actaagggtg ccctccttga aaagatgggt aagatggaaa
     2461 gcgcaataga gttgtataag agtgctgcat tggataatta cgtaaaatct caatatactt
     2521 taggaaatat atatgaaagt aaaaaacaat ttaaagaagc agaaaagtgg tattcgatgg
     2581 catataaaag tggaagtgaa gatgctgctt ttgatttagg taatatgtac tataagttag
     2641 atggctatga atatgctata tattggtatg aaaagatagc aaatttaggt tatttacaag
     2701 cacaaaataa tttaggtgta tgccatttta aaatgaaaga ttttgtaagg tgtgaaaaat
     2761 ggttaaaaac agcagcagac aagaatttag gtaaagcttg ttttaattta ggggtattat
     2821 atactttttt ggagaaagat gatgaagcat atgaatacta taaaaaaggt tctgtgcttt
     2881 tagatgatga tgcaaaatat aatttagcaa tattaaatgg aaaaaagaaa gataataaat
     2941 caacagtaag tttatataaa cagttgtaca agtcaggtca tatgaaaggt tgctttaact
     3001 tgggtatgat tatggaaata aatgataatt tagaagatgc agagaagtat tataaaaaga
     3061 gtgctgatag agatgatgtg aagtctgagt ataggcttgc atatgtatat gatagaaaag
     3121 aagagctggg agatgcaatt tattattatg agaaagcaat agaaaaaaat catacacttt
     3181 ctaagtatag acttgctaat ttatataata gagaaaatga gatagagaag gcaaaaactt
     3241 attataaaat ggctgctgaa gatgatataa cagaagcaaa aaataatctg gcaggtatat
     3301 attttgaaga aaaagattat gaaaatgcta ttaaatatta tgaggatgct atagctgttg
     3361 gatgcaaaag ttcccttgaa aatctagggg atttatatta tcagaatcaa gatatagaaa
     3421 aagcgatttc gtattattcg aggattccaa ataatgtatc ttgtcaaatc aaattaggaa
     3481 atatctatga agatttaaat aatattgaag aagcaataag ttggtataag aaagcatctg
     3541 aaaatggaga tacgaggtct agttatagat tggggtgtat atatgagagc ttaggaaata
     3601 caaaaaatgc tagaaagtat tttgaaatgg caagtagcaa aaaccatatg aatgctcgta
     3661 tacatctagg tagaatttat tttagagaag gtaaattgga agagtctaaa atgatgtttg
     3721 atacacctgc caatgaaaat aatgtgtatg ctcaacatat ggtaggatta atttatgata
     3781 tgttctataa agattatgta aattctaaat tttggtatga aaaggctaga gcacaaggtt
     3841 gtgttgaatc aatatataat ttaggtcaaa tttatttaaa attaaatgat gacgcagaag
     3901 ctgagaaata ttataaagaa ggtattaaat atggaagtaa gaagtgtgag tatatgcttg
     3961 caggtcttta ttataaaaag agcctagata tgtacatttc tttagcaaat gaagagtacg
     4021 ataattgtga agagatagta gaaggctttc caaatttaaa tatagacttt gatgaagtat
     4081 taattcctcc atttaagttg caagaagagg taatagatga agaagagtat gtaccaattt
     4141 atatactaaa tataaaagaa agtttagatt caatgctaga agggcttgaa actgaaatga
     4201 ttatagatga agaacatgat agttaaaaag tattataaga ttatatagat ttaaatatat
     4261 aaaagccatt aggataaatg taaatcaatt aattctaatg gcctttttat taatatttta
     4321 taatagctta tttcttgaca tgaaaaatgt aatcactata catattatag aggtaataag
     4381 aagtattatc caaaatccgt ttgggttatt agaaaatggc atgttgatga cattcatacc
     4441 aaagaatcct gatattattg taggtattga gaaaattagt gtaactactg ttaaaatttg
     4501 cataacatta ttctgattat tacttataat tgcactgaaa gcatccataa ttctactcaa
     4561 tatatctcca tatattttag ccatatcaag agcctgtcta ttttcaacta taacatcttc
     4621 taataggtct tcatcatctg gatacttctt tataccagca gttctaacca ttttgtttaa
     4681 aacaagttca ttagatttta gtgaggtaga gaagtatacc aaagattttt ctaattccaa
     4741 taattgaacc aattctttgt ttttcattga tttgtaaatt tctcgctcta ttatgattgt
     4801 cattttattt atttttctta agtaatgcaa gtaataagct gcatttttgt gaagaatttg
     4861 aagcaaaaag cgagtctttt taaacgtaaa aaaatcttta atatgaccaa ctataaagtc
     4921 atttaaaatc ttagtttgtg catcacaaac tgttacaata gcttcttcag ttagtataat
     4981 acctaagggt attgtagtat aatgagaaga attagagtca ctttcgtcta ctggtatatc
     5041 tataagtata agagtatgat tgtcttcaac atctattctt gagcgttctt cttcatctag
     5101 tgcagattct atgctttcta catctatatt taataaatta gaaatttcgt tgatttcact
     5161 atgattaggt tcaacaagat ttatccaaca gccatcttca aaaaaactaa gtttctctaa
     5221 ttttgaatct atagttttgt aatatcttat catggcaaca catcctttat tcagttttat
     5281 ataaatctca tcatttaata gtatctatta attaactaat ttatataact gaatatatgt
     5341 attagttttg attttagata ttattaagtg atgaaaatgt aaaaaattct ctccgcaacg
     5401 gactagtatc cattgcccat cactctcctt atttattatt ttatatatag ctatttatga
     5461 aatgcgtatt ttaagcactc tttaatataa taccttttcc aggtatataa atcaagtttt
     5521 gatttgatta aataatatct ttaccataat ttacacataa agtatataag ttatatttaa
     5581 tttgagtaaa gaatatatat attctgagtg aaattattaa atatattaaa aaataagaga
     5641 gtatatatat atttacttat ttgcaataat atatttaaaa aagtatttta ttttcaaaac
     5701 tagacttcat ttataattta tcacgaaaat aaaaggaata taaatataag agaatgagag
     5761 gttattagag atatgagtga ttttaaagag attttggcag aagagattaa aaataatcca
     5821 tttggattga taggtaaaga ttggacactt ataacagcag aaaatgaagg taaagtgaat
     5881 acaatgactg ctagctgggg agggcttgga gttatgtggg gaaaagatgt tgcttttgta
     5941 gtaataagac cacagcgtta tactaaggaa tttatagata atacagataa attttctctt
     6001 acattttttg atgaagattt tcgtaaagag ctttcatatt gtggaaaggt atctggtaga
     6061 gaagaggata agatatcaca gattggattt aatatagaac acttaaatga tacaccttat
     6121 tttaaagaat ctaaaatggc tattatatgc aaaaaaatgt atagtcagaa acttgaacct
     6181 caatgtttta ttgctgaagg tatagatgga agatggtatc cacaaaaaga ttatcatact
     6241 ttatatatag ctgaaataac caatgtactt gtaaaagaag attagttata aaggttaata
     6301 ctatctctaa ttaaattaaa atttccataa aaggaaattg acacaaaaaa gctaaggaca
     6361 taaatatgtt tataaagatt aataaataac gtatttaaac tccttagctt gataaaattt
     6421 taatttagtt ttctttaaat tataattcag aaatcaattc attgataaca tcttcaacag
     6481 tacttaattt atcaatttta taggcatcat ttccacaaaa taaaagtgaa ttttcaacat
     6541 ctcctttaac tgcattgatt agagcttgag aaatacagta aggagtttct tttggattac
     6601 aaggaatcaa acaattatag cacttcttta tttctggacg atttatttta acttcttcta
     6661 taaatttgtt tcttatggcg cgtccaggaa gacctacagg acttttaact atatgaacat
     6721 cttcttcact agcatttatg tatgccattt taaagttctc atgggcatca cattcgtaag
     6781 tagcaacaaa tcttgtacca acttgtacac cacttgcacc catatctata tattttttaa
     6841 catcactact agaagttata ccaccagcag caactactgg tatgtttcta ccaaattttt
     6901 ctccataagt atttgcaacc tttaaaattt caacaaattc cttatcatag tctatagaat
     6961 ctatgttatc aagttcctcg ttagagtatc caaggtgacc accagctttt ggaccttcaa
     7021 caactattaa gtcagcagta gttttctctt ttctatccca catttttaaa attacattag
     7081 cagcttttgc agttgaaact atgggtgcta tttttacatt acttcccttt actaatgatg
     7141 gtagcttatt aggtaatcca gctccagata ttataaggtc tacaccagct ttaacagctt
     7201 cctttacgta tgtttcatat tctttcatag ctaccataaa attaatacca ataataccac
     7261 catttgagat ttctttggcc ttagaaatat gtttttttat agctcttaaa tttgcattta
     7321 tagtattagt ttcaaaatct tcctcatcat atccaatttg aactccagaa attacaccaa
     7381 caccaccaag cttagcaact gcaccagcta gtgatgacct agatatacca acgcccattc
     7441 caccttgaat tataggaact tttgcaacta aatctctaat tattaactct tttatgataa
     7501 tccctcctca atgtaaacat gtttaaagaa ctagattata atacttaata ttattaccta
     7561 gtcctaatta ttatactaca aattataata aaagagtagt tatattttgt atagaagacg
     7621 ataattatat tatatttaaa acagctccat tagaatcgca acataactct tttatttccc
     7681 aatcagattt tagtttttgt aggtaatttt tcatattatc cacaaaacta tcatcatttt
     7741 cttttattaa agacattata gtaggtccag ctccacttaa aaatactcca atactattta
     7801 attgttcaga tttttcaaca atatcattgt agttctcaat tagagttcct ctataatctt
     7861 gatgcaattt gtcttgacaa gctactttaa gtaggtcaaa gtttttgttt agtaaggcag
     7921 atattagtaa agcaactcta ctaacattga ataccccatc agaatatggt atttcctttg
     7981 gtaaaacacc tctagctttt tctgttgata acttaaaatt aggtatcatt gcacaaaatt
     8041 taagttcttc aggaattttt ataatatcat aatgtatatt ctcattatca gtaacagata
     8101 caatcatatt tcctaaaata gcaggagcaa cattatctgg gtgaccttct atttctgaag
     8161 ctatatttag taattgattt ttatctaaat tagcacctga tagagcattt gcagctataa
     8221 ctccagctac tatacaagag gcactactgc caagacctct acatataggt acttcacttt
     8281 ggatttttat tttgatacca gcaggtattt tttctggttt aactctatca aaaaagtact
     8341 tcatagatgt ataaactaag ttgttttcgt ttttgtaagc atcttcacaa ccttctattt
     8401 caagaccact ttcaatttct tcaacataaa atttattgta tatatttaac gcaattccaa
     8461 gacaatcaaa tcctggacct atattagcac ttgttgcagg aactataatc tctaacataa
     8521 taatcaccta tacctttaag aattttttta tagctagttt taattcatct ttttcataaa
     8581 tatctttatg tagtatttct gcatttttaa gatttttaat aggttttgga atatctattt
     8641 tagaaatact tgataatcta tcaattatag caaagtcatc aaggtcttta aaatcacaat
     8701 ctatagactt tagaacatct tcagaaaact tgaatggact tgcagtactt acaataacag
     8761 tttttgtgtt atcagaagtt tcttttttat atttttcata acaattgtat gcaacagcag
     8821 tatgagtatc tatcaaataa ttataatttt taaacacatt gtttatcgtg cttttaactt
     8881 cttcttcatt tgaatattct ccatagaatg aggctaaatt tttttccata tcatcactta
     8941 ttttgtagac tcctttatca ctaaggtctg ataaaagctt atttacaata tcagtatttc
     9001 tatttgatat ttcaaacagt aatcgttcta aattacttga aattaatata tccatagaag
     9061 gagaagtagt taatttcaat gttctatttt tatcatatac accagtttta aagaaatcat
     9121 aaagaacatt attatcattt gatgcacata ttaatttgtt tattggaaga cccatttgtt
     9181 tagcataata tccagctaga atattaccaa aattaccagt tgggacaaca aaatttattt
     9241 tatcatttaa atttatatca ctattgctaa ctaatttcat atatgaataa aaatagtatg
     9301 caacttgtgg aagaagtctt cctatattaa ttgaatttgc agaagataat atataattat
     9361 tatccaatag tttttgatta aattctttat cagaaaaaat cttctttaca cctgattgtg
     9421 catcatcaaa atttcctttt ataccaacta catatgtatt ttttccagtt tgagttttca
     9481 tttgaagttt ttgtactggg cttacaccat cttctgggaa gaatacaatt atctttattt
     9541 tatctatatc tttaaatccc tcaagtgcag ctttacctgt atctccagat gtagcagtaa
     9601 gaatcacaac atctttctct aaattatctt tctttataga tgttttaaga agatgaggca
     9661 ttatagtaag tgccatatct ttaaatgcta aagttggacc gtgatatagt tctaagaaat
     9721 aagtatcatt tactttattt aatggagcta tactactaca atcaaacttt tcatcatatg
     9781 cattttctat acaattttta atttcatctt cagtaaaatc acataaaaat ttactaagta
     9841 caaaaaatgc tatctgagaa tacgttaaat ttactaaatt tataagttca ttttttacat
     9901 tgggaaattc acttggaaca taaagaccac catcatttga tattcctttt attattgctt
     9961 gtgatgcagt tactttttct tcggtgcctc tcgtacttga ataatacatt ttttataatc
    10021 ctccatatat tttaaatttt taattagcta attaaattag atacagaata ttttaaaaac
    10081 tgaatttagg taataaaaaa agaccctcat ccctaaaaaa gggacgagaa tctgtttccc
    10141 gcggttccac ccaaattgat tttaaaaaat ccacttgata ctttaacgca agtaataaaa
    10201 acgtctattc ctactaaaat cattaagata acttcggata gaagctccta ggtagttttc
    10261 aatccttcta attagggaaa catttcagct agtagtttcc ttctctggta atcgattcag
    10321 atttactctt cctaatcaat gcctttcaat attcttaata ttattaatta ttctagttta
    10381 taaaacagac aaagtcaagt attaatataa aaatttggaa aaaatatttt ttaaattgta
    10441 aaaaaattgt aatcaaattg agtattagaa aaatattttt ttaacttttt taaaagttta
    10501 aaaacctaga ttttcatagt gatgaagctg tttttaatga aagtaattaa aagataacta
    10561 ttaaatatct gtaatattac
//
LOCUS       DAELTA010000130        10376 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.130, whole genome shotgun sequence.
ACCESSION   DAELTA010000130 DAELTA010000000
VERSION     DAELTA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.130"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(302..1552)
                     /locus_tag="KPG13_002801"
     CDS             complement(302..1552)
                     /locus_tag="KPG13_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG0986501.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1584..2525)
                     /locus_tag="KPG13_002802"
     CDS             complement(1584..2525)
                     /locus_tag="KPG13_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG0986502.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2765..3841)
                     /locus_tag="KPG13_002803"
     CDS             complement(2765..3841)
                     /locus_tag="KPG13_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG0986503.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4214..4729)
                     /locus_tag="KPG13_002804"
     CDS             complement(4214..4729)
                     /locus_tag="KPG13_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG0986504.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5092..6162)
                     /gene="leuB"
                     /locus_tag="KPG13_002805"
     CDS             complement(5092..6162)
                     /gene="leuB"
                     /locus_tag="KPG13_002805"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG0986505.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6169..6660)
                     /gene="leuD"
                     /locus_tag="KPG13_002806"
     CDS             complement(6169..6660)
                     /gene="leuD"
                     /locus_tag="KPG13_002806"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG0986506.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6675..7952)
                     /gene="leuC"
                     /locus_tag="KPG13_002807"
     CDS             complement(6675..7952)
                     /gene="leuC"
                     /locus_tag="KPG13_002807"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG0986507.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7909..9651)
                     /gene="leuA"
                     /locus_tag="KPG13_002808"
     CDS             complement(7909..9651)
                     /gene="leuA"
                     /locus_tag="KPG13_002808"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG0986508.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9775..10021)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10169..>10376)
                     /locus_tag="KPG13_002809"
     CDS             complement(10169..>10376)
                     /locus_tag="KPG13_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986509.1"
                     /translation="NNQCIFSIKSKQSQKFNYKIIEKIEVVKNILNIYTDESNYKIRL
                     RALSLEDEKKLLNIIDEKMKKFKA"
BASE COUNT         3141 a         2001 c         1065 g         4169 t
ORIGIN      
        1 aaatataaat ttcaaaatca ctttttttat taactttttt ataattataa taaaaatata
       61 taacagtact attataaata cacatttaaa accttataaa tatacaaata caattatatg
      121 aattttcatc ctcaattata gcatagaata tgactatcta tatcctaaaa ttatccattt
      181 ctaaaaaaaa ggtaactatt attaagaata tcaacttata gaatttatat tccacatacg
      241 attttcaatc aaaaaaacaa gggctaaaaa gtgaattgtg cactttttag ccctttatta
      301 attattcatt tatttcatgt aagaatagac cacatcttac tttaggttca aaccatgttg
      361 acttagctgg cattatctta tttgcatctg ctactcttat taaatcttca atactagtag
      421 gatacattgc aaatgctact ttcatatctt cattaactct tttttctaat tcttcaagac
      481 ctctaattcc accgataaaa tcaattcttt tatctactct aggattatca attcctaaaa
      541 tagcatcaat aacatagtct tgtaatatag aaatgtctaa tgagtcaaca gggtcttcta
      601 tttctaataa atttttattt gctttcattt tataccactt atcacctagg tacattccga
      661 aagttccttt tttgtcaggt ttatattttt cttttccttc tatttcttct agttcaaaat
      721 tttcttttat tttatttata aattcttcct cgcttaaacc atttaagtct ttgataaccc
      781 tattataatc caaaaccatt aaattctcat ctggtgcaat cattgctatg aagtaattaa
      841 attcttcttt tccagtataa tttggatttt tagctctcat ttctttggct acattttctg
      901 ctgaagctgt cctatgatga ccatctgcaa tgtatagata atctaatttt tcaaatttat
      961 ctactaggtt atctattata tcatcattat caatcttcca tactgtatgc tctatcttgt
     1021 catctgttat gaaatcatat aaaggttcat tattatccat aaaatcattg attatatcat
     1081 ctatcaccct ttgatgtttg taagttacaa gtatagttcc cgtattagca tcacagtatt
     1141 ttatgtgttt agttctatca atttcctttt ctggtcgagt gtactcatgt ttttttattg
     1201 ttccattcaa gctttcatca actgatatac atgctactat accaacttga gctctaccat
     1261 ccatgatttg tttatatata tatatacact ctttgtcatc ttttactaga atttcttgtt
     1321 ttcgaaactc atctaaattt tctcgcgctt ttaaataaac tttttcatca tataaatcta
     1381 tattttcatc taaatcaatt tctgatttat ctatatgcaa aaatgaatat ggattccctt
     1441 ctgcaattct tctagcttct ttactattca taacatcgta aggaagtgct gctactttat
     1501 ctactatata tttatttggt ctaatcgcct taaatggttt aatcgttgcc attactaatt
     1561 tcctcctaat tatacaaaat attttataat gctaccccta ttagattgtt gaagtcaaga
     1621 aagaaatctt gaatcacatt tactatttcg tctcctattc tttcttgagc ttctactgta
     1681 gatgccccaa tatgaggtgt aggagaaact cttggatgat ttactaagtc taaatttaat
     1741 gtaggttctt gttcataaac atctatagca gctgctgata atttaccttc atctaatgct
     1801 tttagtaaat ctttttcatc aactaaaccg cctcttgcac agtttattaa ataagcccct
     1861 ttcttcatct tatttatttc tttttcagtt attactgccc ccttattctt atcaaatggt
     1921 atgtgtaaag ataagaaatc tgcattttct aaaacttctt ccatatcaca gaatttataa
     1981 ttgttaaagc cttgcgcctc acccattatg tcagtatata taacattcat acctaataat
     2041 tctgctctct ttgcaacttc ttttgcaatt cttccaaaac ctataagtcc aagtgtcttt
     2101 ccattaattt ctgtaccttt atactttttc ttctcccatt ttccatctct catagttaca
     2161 tttgcagtat ttatgaatct tgcaagaaca aataattgac ctattgtaag ctctgcaaca
     2221 gatatactgc ttgcatttgg agtattcata acttttatgc cattagcttg agcatattta
     2281 acatctatat tatccagacc tactccacct cttactatca acttcaactt tttaccctct
     2341 aatgctttat ctattacatc tttagtaact ttagtagctg aacgtactac aagtacatca
     2401 acatctttaa gtttatctcc taaaacatct ttttcataaa actgctctat cacattaaaa
     2461 tctaactctc tcaattttct cgctgcttct ttctctattc catcagtaac taatatatta
     2521 tacataacaa actcctccaa tttttattca cttaattttt ccaattttta ttttttaaag
     2581 tattttcatc aataaaaaac tttacttaat ttaactttct gtttatataa ttttcaaaat
     2641 atttttttat taaagtcata tcaaattttt ctattgtttg ttaaaaattt aatatgtaat
     2701 ttttataaaa tattatttat tatatattct aaatttatct taaaacatac atatttttac
     2761 aatattattt attaaaacct aatatatcat caatattatc taataattct tgtacatctt
     2821 cgacagtata gtcacccata tgtgaaattc taaaagtttt ttcttttaaa tctccatagc
     2881 catttgctat ttggaatcct cgttcttgta attttgaatt taaatttgat acactaatac
     2941 cttgagtatt ttctataaca gtaagtgtat tggataagtg attttcattg gtaaaaattt
     3001 ggaagtgttt tttagcccag tttctaacta agtttgccat gtcttcatgt ctgttgaatc
     3061 tgttatctag gccttcatct aatatattat ctaattggaa atctaaagcg aacatgtgag
     3121 aaagagatgg tgttgaaggg tattggtagt ttttcttgat taagtattca tacattgcta
     3181 acaaatcaag atatactcct ctaaaaggta cttgcttagc tctatcgatt gctttttgag
     3241 aaaatgtgca aatagacatt cctggtggta atcccagtgc tttttgtact gatgttatac
     3301 aaatatctat tccaatctta tcaacctcga cttttattcc accggctgaa cttacagtat
     3361 ctacacagta gattacatcc tcatactttt taacaacttc tccaatttct tctattggat
     3421 ttcttattcc agttgaagtt tcattgtgag ttacagttat taaatcgtat tctccggtag
     3481 aaagcacctt atcaaccatt tctggcgttg ttgcctctcc taattcaact ttaaatatat
     3541 ccgctgggac attattagcc actgccattt tgtaccatct atcaccaaag gatccacagg
     3601 aaaatactgc tgcctttttg gatgtacagg aacgaatgga gccttccatt aaaccagttc
     3661 cggatgacgt ggataaaagt atcgtgttgt ttgtatagaa tagtttttgc atcttttcac
     3721 ttatactctt ttggagcata gatgcatctt ttcctctatg tcctatcatt ggtgtagcca
     3781 tcttttgcaa gacttcttct ttaacatcaa taggaccagg aataaataat ttcttactca
     3841 ttataattcc ccccttttta gtttgtaata tggatagcgt ttttactgtc cttattttta
     3901 ataactataa tatcaagctg aattaactat gtcaacataa aatttatttt ttatatataa
     3961 tttattttat atattttatg aaaacattca ttttctacct tttcataagt ctgaattttc
     4021 tgaattattc cctcaaaaaa aatatacttt ttatctatat aaattaattt ttttagtatg
     4081 taaattttac gctattacga taaatctttt tagtataact aatttatttt tatcttgtat
     4141 ttttattgta tttttgaatt ttattttttc taaatataaa atttatgttt taaaatataa
     4201 aatttttatt taatcaaagt tcaatatatt ccactttttc tttcgattct tcaaaaaacc
     4261 attcttcatt agaaaaatca ccttctgcaa tttgtccaat aggcattttc atatactttc
     4321 cagttgctgt cactgctata tctccatttg gtagaattat ttctcctgta ccttcaaata
     4381 attttctgtt ttctttagtt attcttccaa caacttttat agtagcatct gttggtactg
     4441 gtttcttata tttcaactct aaagatactg ttactcccca tatttggtca tcatttatac
     4501 ttacagctct tccaatagtc tcatccaata ttgcagcaga catacctcca tgaactctcc
     4561 caggataact ttgatgccaa tcctttgtat tacaaattga tacaagctct ccattttcaa
     4621 gttcataaaa atctgcttta agtcctagct cattttgtgt tccacaaact aaacaagatt
     4681 tactactatt ttgcttcttc aaaactttat actttgccaa aaaatccatc tcctaaccga
     4741 cattagtagt cgcttaatat taaataattc taaaatatat tatataatat taataaaatc
     4801 cctgcaaact tctaataata aaaataggct acttcaatat gataagttta atttaaagta
     4861 ttattttcca aaaaataact ttatcaaaat agaaactcaa agtagatata aacctctttt
     4921 tcgttattaa aatagtaaaa atatatttga cttaatataa aaaagagtgt ctcattaact
     4981 aataaagttt attttgagac acccctcata aaacaaatta tactacaata gatttttact
     5041 aattctacaa gttatatatt taaattatat tattcaaatt aaactataat tctaaatttc
     5101 atttactatt aattcgccca tttcccttgt tcctacaaca tttcccacac cattatagat
     5161 atcaatagtt ctataacctt tattgagtac actttgtaca gcatcttcaa tacacttagc
     5221 ttcttcttct aagttaaaac tatgtcttaa catcatgctt acagatagaa tagtcgcaat
     5281 tggatttaca atatcttttc cagctatatc aggtgcactt ccatgtattg gttcatacat
     5341 accaaaacta tcttctctaa gtgatgctga tggtaacata cctatagaac ccgtaatcat
     5401 agatgcttca tcagatatta tatctccaaa tatattatta gtcactatta catcaaattg
     5461 gctagggtct tttacaactt gcatagcagc attatcaaca tacatatgtg acagttctac
     5521 atcttcaaac tctttagcta agtcttccat tatacttctc caaagtcttg aactctcaag
     5581 cacatttgcc ttatctacac ttataagttt tttatttcta tttctggcta tttcaaaagc
     5641 tctcttacca attcttctta tttcattttc gttatatatt tctacatcat aagcattttc
     5701 gataccatca atgatttttc ttccacgttc tccaaaataa atgccccctg ttaattccct
     5761 tacaacaaca aaatcaatgc ctttctcaac tatatccgtt ctaagtggtg aagcttcttt
     5821 tatactttca tacattgtag ctggtcttaa atttgcaaat aaacccaatg actctcttaa
     5881 ttttaaaaga ccagactctg gtcttttatc tcccttacat ttatcccact ttggtcctcc
     5941 aacagcacca agcaatacag cttcgttttt tcgacaagtt tcaacagttt tattaggtag
     6001 aggttctcca gtttcatcaa tcgcgcatcc tccagctaat acatattcac aatcgaatct
     6061 atgattaaat ttacaacaaa ttttatttag aacctttata ccttcatcta taatttccgg
     6121 tccaacacca tcacctttta tgactgctat attacaattc atacttcact accccctatt
     6181 tgcttttatt gagttaatta agccttcatt taatataata ttttgcataa actcaggaaa
     6241 tggttctgct ttatattctt ttccttttgt tatatttttt atcacacctg tagaaaaatc
     6301 tacttctata ttatcccctt catctatgtt attggcagct tcttcacatt caagtatagg
     6361 aagccctata tttatagagt ttctaaaaaa tattctagca aaagtagaag caatcacaca
     6421 ggatactcca ctttctttta ttacaattgg tgcatgttct cttgatgaac cacaaccaaa
     6481 gtttttagtt gcaactataa tatctccttt ttttacctta gaaataaatt tatcatcaat
     6541 atcctccata caatgcgttg ctagttcttt ataatcagct atatttaaat atctagctgg
     6601 tattattaca tctgtatcta tattgtcacc gaatttaaaa acacttccat tggcaatcat
     6661 aaattttacc tcccttaaat tatttctgat ggttttgata tttttccagt tatagctgat
     6721 gctgctgcta cagctgggct tgcaagatac acctctgaat caacatgccc cattcttcct
     6781 acgaaattcc tatttgttgt tgaaatggat ttttctcctg ctgcaagtat acccatatgt
     6841 ccccctagac atggaccaca agttggtgta gaaactgctg ctcctgcctc tataaaagtg
     6901 gtaatatacc ctttttctat agcttcaaga tatacttttt gagttccagg aaatatcata
     6961 actctaactc catctgctac ttttttgcct ttcattatct cagctactac ttcaaggtct
     7021 gatattcttc catttgtaca agagcctatt acaacttggt ctatctttat atccttatct
     7081 atctcatcta ctgttctagt attttcagga agatgaggaa aggctactgt ttcttttagt
     7141 tcagctaaat ctatttcgta tacttcatca tagactgcat cttcatctgc ttcatatata
     7201 tttacatctt ttgtcatagt tgaacactta tgtgaattta tatattccat agtcttatcg
     7261 tctactggaa aaattccatt ttttgctcct gcttctattg ccatattaca aatagtaaat
     7321 ctatcatcca tagataagta ttctactcca tctccacaaa attccattga cttgtaaagt
     7381 gctccatcaa cacctatctc ccctattata tgaagtatta tatctttacc acttatccat
     7441 ttatttggtt tattcttcaa aacaaatttt attgctccag gaaccttaaa ccaacatttt
     7501 cctgtagcca tacctgctcc catatcagtt gaacctacac cagtagaaaa tgctcctaat
     7561 gctccatatg tgcaagtatg agagtcagca cctataatta catctccagc agttactata
     7621 cctttttcag gaagaagaac atgctctatt cccatatctc ctacatcaaa aaaatttaca
     7681 actccttttg attttgcgaa acttcttgta aatttacatt gttctgcact ctttatatct
     7741 ttattaggtg taaagtggtc taaaaccatc actactttag tcttatcata aacatcttca
     7801 attcctaatt tttcaaattc tctaatggct actggtgatg ttatatcatt acctaaaact
     7861 acatctaaat ctgcttcgat taattgccct ttcttaactt catttaattt agcatgtttg
     7921 gctaatattt tctgcgtcat agtcatcccc attttttatt tcctcctcaa tataactttt
     7981 atttattgca ttcataagtg cattaatagc tgctgtattt atgttttccg atatacttac
     8041 accaaaatag acttcattat tgtgctctat tcctacataa gctacccctc ttgaatgtga
     8101 cccttcctct aatgcatgtt cccagtaata tctaaattta taatctttca tgccacattg
     8161 ctttagtgca ttattaaatg aatctacagg tccatttcct ataccaacaa tcctttgttc
     8221 atttcccata taacttattc ttgcagtcat ctcaatcatg ttagtatcat tagaatcatc
     8281 attttcatag tttaattcat ccattgattt tattttatac ttcttaactt tataagggct
     8341 ctcaacttca atatattctt tcttaaacaa attaaatatt gcttcactac taagttcttc
     8401 cccaattcta tcagattcca ttttgactac atttccaaaa tcagaatgca tattcttagg
     8461 tatcataaat ccataatctg tttccattat ataaacagct cctccttttc cagattggct
     8521 atttatcctt ataatttctt tgtattctct tccaatatca tgagggtcta ttggtaaata
     8581 tggaacttcc cagtaatcat tatctctgtt tttcattgct atcatgcctt ttctaatagc
     8641 atcttgatgt gaacctgaaa aagcacaatg tacaagatta cctgcatatg gatgtctatc
     8701 atgtaccata agttttgtac aatcttcata tatacctatt atcttatcta tattgctaaa
     8761 atctagctct gggtctattc cttgtgtata aagattaagt cccacattta ctatatccat
     8821 attcccagtt ctttcaccat ttccaaatag tgttccttca agtctatcgg caccagccaa
     8881 taatccaagt tcacttgcag ccgtacaagt tcctctatca ttgtgagtat gcaaacttag
     8941 tataatagaa tccctacaac ttatattttt acaaaaccat tctatttggt ctgcatatac
     9001 gtttggtgtt gccatttcaa ctgttgatgg aagatttatt attacttttt tttgagtagt
     9061 tggtttccaa acatctatca ctgcctcaca tatttcaagt gcataatcca tctctgttcc
     9121 tgtaaaactc tctggtgaat attcaaaagt aaattctgtt tctggatatt tttttacttc
     9181 ttcattaaat aattttgctc cttcaactgc tatatctatt atttcttgtt tactcatatt
     9241 aaaaaccaca tctctttgaa gaacagatgt agagttatat aaatgtataa ttgctttttt
     9301 acatcccctt attgattcaa atgttttttc tataagatga gccctagatt gtgtaagtac
     9361 ttgtatagtt acatcatctg gaatcatatt ctcatctatt aattttctta aaaatgtata
     9421 ctctgtattt gatgctgatg gaaatcctac ctctatttct ttaaatcctg tatcaataag
     9481 cattttgaac attctgactt tttcatttac actcataggt gttggcagtg actgatttcc
     9541 gtctcttaaa tcgacactac accaaattgg agccttaaat atttcattat ctggccattt
     9601 acgatttact atttgcattt tatcatattt tttatacttt ccacacttca tgttatttcc
     9661 ccctttttct gttttttgta ttcagtatac ctcatatcga cgcaatcctg cacaattcat
     9721 aaggataaat gtcaatttta tcattagaat aaaaaaaacc tcatctctaa taaaattaga
     9781 gacgaggttt tgctcgcggt accactctat ttagctttct aaaaagctcc cttagtaata
     9841 gccaatacaa taattataat gactatattt cctataacgt ggaattactc cgattattct
     9901 aagatttaaa tcttagaaaa cagctcctag acaagttcag aactatcaac tactaaatca
     9961 caccatcctt tagctctctg aaagtttcat tgttcttact acttctattc atcacttttt
    10021 taatcattta aattttctaa atattttgat ttaatagtta ttatatacac attaaaatgt
    10081 attgtcaaca caaaaaataa ttatctcaat tttttatttt tgataagcaa ttctataaaa
    10141 catgctaact attgaaaaca gtatgatatt atgccttaaa ctttttcatc ttttcatcta
    10201 tgatattcaa taattttttt tcatcttcca aactcaatgc ccttaatcta attttataat
    10261 tagattcgtc agtgtatata tttaatatat tttttactac ttctattttt tctattatct
    10321 tataattaaa cttttgactt tgtttagatt ttatactgaa tatacattga ttattt
//
LOCUS       DAELTA010000131        10288 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.131, whole genome shotgun sequence.
ACCESSION   DAELTA010000131 DAELTA010000000
VERSION     DAELTA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.131"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            254..1678
                     /locus_tag="KPG13_002810"
     CDS             254..1678
                     /locus_tag="KPG13_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG0986510.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1713..3842
                     /locus_tag="KPG13_002811"
     CDS             1713..3842
                     /locus_tag="KPG13_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG0986511.1"
                     /translation="MKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDVVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3872..4294
                     /locus_tag="KPG13_002812"
     CDS             3872..4294
                     /locus_tag="KPG13_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0986512.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4313..5464
                     /locus_tag="KPG13_002813"
     CDS             4313..5464
                     /locus_tag="KPG13_002813"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0986513.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5570..5671
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5854..6426
                     /locus_tag="KPG13_002814"
     CDS             5854..6426
                     /locus_tag="KPG13_002814"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG0986514.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6490..7140
                     /gene="fsa"
                     /locus_tag="KPG13_002815"
     CDS             6490..7140
                     /gene="fsa"
                     /locus_tag="KPG13_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG0986515.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7292..10216
                     /locus_tag="KPG13_002816"
     CDS             7292..10216
                     /locus_tag="KPG13_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0986516.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4219 a         1005 c         1934 g         3130 t
ORIGIN      
        1 atttaactga attattattt tcatttctat taaatttaaa tattttataa aaatagtatt
       61 atttacaaaa tcatatactc caaaatacta cataaataaa attaaacaaa aactaaaatg
      121 acaaatgtca ttatctaaag tgacatagaa acggtttctt aaagttgtta ctataaaata
      181 taaactctat aataaaagta taacaaaagg gaatcatatc gatatagaat ataattcaag
      241 aggaggtaaa attatgagta gttctaatca atcgacaata aagacatcta tacagcgagt
      301 agggaaattt ttaagtggta tggtaatgcc aaatatagga gcttttatag catggggtct
      361 tataacagca ttatttatac caacaggatg gatgcctaat gaaaaattga gtactatagg
      421 tgacccaatg ataaaatacc ttttaccact attgatagct tatactggtg gtaaagcaat
      481 agcaggacaa agaggtggcg taataggtgc agcagcagct atgggagtaa ttgttggtgc
      541 agatatacca atgtttattg gagcaatgat aatgggacca tttgcaggat gggtaattaa
      601 gaagtttgat aaatttgtag atggaaagat accaacagga tttgaaatgt tagtaaataa
      661 cttttcaata ggtataatag gtatgcttct tgcaatacta ggattttatg caattggacc
      721 agcaatagta gcagggacag ctcttataga atctggagtt caatttatag ttagcaaaag
      781 tttattgcct ctagtttcag tatttataga acctggaaaa gtattattct taaacaatgc
      841 aataaatcat ggtatattag ggcctatagg tatagctgag gcaaaagaag caggtaaatc
      901 tataatgttc ttactagaaa gtaaccctgg accaggttta ggagttctat tagcatattg
      961 gatgtttagt agaggaagtg taaaacaatc agcaccagga gctgtaataa tacatttctt
     1021 tggtggtatc catgaaattt acttcccata tattttaatg aatccagttt taatattggc
     1081 tacaatggca ggtggagcag caggaatatt gactttttca atattaggag ctgggcttgt
     1141 agcagcacca tcaccaggaa gtatatttgc acttatggca ctagctccaa agggaggatt
     1201 acttccagta ttagcaggag ttgcagtggc aacagttgta tcattcttag tggcagcacc
     1261 atttgttaag agagcttcag caaatcaaag tgaagaagac agtacttctt tagaagaagc
     1321 taaagcaaaa atgagtgata tgaaatctgc aagtaagaac tctgagaaaa atattgagga
     1381 aaaacagctt gaagtaaatg agattaagaa gattgtattt gcatgtgatg caggaatggg
     1441 ttcgagtgca atgggagctt ccagatttaa aaatagaata aaaaacttag acttaaatat
     1501 agaaattaca aattcatctg ttgataatct tccagatgat acacaaatag tagtaactca
     1561 caatacatta gttgaaagag ttgctaagaa taattcaagt gttgagatag tttcaataaa
     1621 caatttttta aatgacccaa atttagatac attgtttaaa agattagaat ctaaataaaa
     1681 ataaaaagta ccactaatta ggagtggata atttgaaaaa aaagaagatt acatctaggc
     1741 aaaaaaagat aatactaatg atagtagcaa attcaaagaa aaacattcca ataactattt
     1801 ctgagattgc agggacttta gagttaagtt caagaactgt acttagagat atgtctggaa
     1861 ttgaaaaatg gtttgatgaa aatgatttta actttgtaaa aaaaccagga gtgggattga
     1921 ttttagagga aaatatagaa aatcaaaact ttataattga gttgttagaa gaagaaaaaa
     1981 tagaaaaaga atacagcaag gaagaaagaa atttaattat attatcaaag ctactagttt
     2041 ctaacgagcc agtaaaatcc tattattttg caaaaatatt aaaggtatct gaatgggtac
     2101 tgaataatga ttttgcctta gcaagtaaat ggttggaaag atttgatatt gagcttgtta
     2161 gaaaaccagg gctcggtgtc tacttaaaag gtcaagaaaa aaactttaga gaggcatatg
     2221 taaatcttat atacgattca ttcaatgaaa aagaaatact agatatggtc agaaatataa
     2281 gtgaaaatat acaaacagat aaagcaatag aaattttaag tgaaaataga cttttaaatt
     2341 taatggacag gtgcataata agaaaagttg aagctacatt aacaaagaag ctttcagatt
     2401 tagatgtaaa tttagctgat agtgcatata tagggcttgt agttcatatt tcattagcct
     2461 tacagaggat aaaaaatggt gaaaatataa ctatggataa agagtttcta aaagaattat
     2521 ctataacaga agagtttaaa ttagctaaag aaatcgtaaa aggtatggaa acagatttta
     2581 acatggatat accagtagat gaggttggat atataactat gcatataaga ggtgcaaagc
     2641 aaaggtcatc ttcaaaccat aaggctttaa acttagatga tatagaaata atggaaatta
     2701 caaataagat gatagatttg gcggaagatg aatttaaaat aagtttaaaa aatgacgaga
     2761 ggttatttaa agacctagca aatcacttag ggccttctat aaataggttg aatatgggat
     2821 tagaaataag aaatcctctt ttagatgaaa taaaaagcaa gtattcatat gcctatgatg
     2881 tagttgaaaa gatttctaga attataaaag ataaactaaa tataaattca atacctgaat
     2941 cagaaatagg atatattgca atgcactttg catcagccat agaaaaaaat cttatgatga
     3001 atactaatat aaatattgtt gtggcatgtc ccactggaat aggtacttca agattcttat
     3061 caactaagat tgaaaataaa tttcctaatt taaatatact tgaaaccata tcagctataa
     3121 acatagatga agagtattta aaggaaaaag atgttgattt aatagtgtct acagttgagt
     3181 taaatacaag tttaaattat atatgtgtag gtccatttat gagtttagat gatgaacaga
     3241 taatcaagga aaaaataaaa tctatagctc aaaataaatt aatcaattta aatgcgaaaa
     3301 atgatactaa gagtaagaat aaagtatatg aacagataac agaaagtatg aatataggaa
     3361 aagatattct tcaattttta gaggaaatta gatttgaaaa atttgaaagt aaagatttaa
     3421 gtgagcttat tgaagactca tcaaaaatat ttgcaaaatc tagtgaagat ataatctcta
     3481 taaaagaatc gttaaaagaa agattaaaaa tttcaattcc atatatagaa gaatcaaaaa
     3541 ttctattgtt acattgtatg agtgaaagaa tagatattat gaagttatca ataataaaac
     3601 tagagaatag aatcgtgcta gattcaaatg aagaaatcga caatgtagtt tttatgttac
     3661 ttcctaaaaa ttcaccaagt tatcaaagac aaataatgag tgaaataagt ggttcattaa
     3721 tagacaatat aatttttact aataagataa ataaattctc tatagaagag atggcattag
     3781 aggtaaaaga tatagttttt aatttttata caaacagatt aaaagcattt atagataaat
     3841 aaaattaata ttaaaaagga aggttgatac tatgagtaaa gtattgaatg agaataatat
     3901 atttttaggg ttagatagtg tttcaaagga agaagctata actttagcag gaagaaaatt
     3961 agttgaaaat ggatatgtaa aagaagaata tataccagca atgttagaaa gagaaaaagt
     4021 aatgactaca tatatgggaa tgggagttgc tattccacat ggtgtaaatg aagcaaaaaa
     4081 agaaatatta tcttctggaa tagttatatt acaattccct aatggtatag attttgatgg
     4141 agaaaaagca tatttattaa taggaatagc tggtgttgga gatgaacatt tagaaatact
     4201 atctaatata gcagtagttt tagatgatga cttaacagaa agactaaaaa attcaaatga
     4261 caaacaagct tttatggaag cttttgccaa ctaatcttag gagggattta aaatgaaaaa
     4321 ggcaattcag tttggagcag gaaatattgg aagaggtttt attggaggct tgttagttaa
     4381 gtcgggttat catgtagtat ttgctgatgt aaatgaggag atattaaact caataaacaa
     4441 agataaaaag tatactatac acataagaga tgtagaatgt atagatgaag taattgataa
     4501 tataagtgcg gtttcatcta taaaagaaga aataatagat gagattgtac aggctgaaat
     4561 aataactaca gcagttggac ctttagtttt gactaaaata gcctctacaa ttgcaaaagg
     4621 tataaaggct agaaaagaaa agggattaac aagtaatcta aatataatag catgtgaaaa
     4681 tgcaatatac gcaagttctt ctttaaaaga agaggtactt aagtatttaa ataaggaaga
     4741 agtagaatat ttagagatgt atgttggttt tccaaactgt tcagttgata gaatagttcc
     4801 tcctggtaaa aacgaaaatc ctcttgatgt aacagttgaa aatttttatg agtggaatgt
     4861 tgaaaaacaa ggatttaaag gggaaatacc aactatagtt ggtatgaatt tagctgataa
     4921 tcttatggct tatatagaac gtaaattgtt tacactgaat acaggtcatg ctataacagc
     4981 ttatatagga tatttaaaag gatataagac tatagaagag agtataaaag ataaatttat
     5041 ctgtgatata gtcaaaagtg caatggtaga aagtggagaa ggattgatta aaaaatacaa
     5101 ttttgactca gaagtgcact ataaatatat tgataaaatt ttaaatagat ttaaaaatcc
     5161 atatcttaat gatgatgtat taagagtcgg aagggaacca ttaagaaaac taagtgataa
     5221 agatagattg attaaacctt taatgacagc aaaatcatat ggattatcag ttgataattt
     5281 gattcttgga ataggtgcag cgcttcatta taataatagt gaagatactc aaagtgtaga
     5341 attacaagag ttgataaagt ctattggagt aaaaaaagcc gttgctaaaa tagctaatat
     5401 aagcaatgac gaagaacttt taaacaatat cgaaaaaagt tatatattta tgaaaaattt
     5461 ataaatttac ttgaaaaaaa gtagttctga taaagtaact ataatatatt attctacaca
     5521 gtttgaatta tttgttgaat aatagagaat tataatatat aattaaaagg aataaattaa
     5581 tatatttacc tgtatatgtt tgataatatg gttcaaatgt ttctactaag ctaccattaa
     5641 tagcttgact acaggagtga agaagatata gtttatactt attttaaatt atatattaag
     5701 ttaattactt tgaattttaa gctcctgtct atgatataga caggagcttt tttagtattt
     5761 taatataaaa tacttacaag aatagatagc aaaattagaa caaatataat ttttttatta
     5821 atataatttt agaaagggct aggtggaagt tcaatgaagg ctttacaaga aaagatattg
     5881 agagaaggca gtgtttcagg aaatgatata ctaaaagttg atagtttttt aaaccatcaa
     5941 attgatgtag cttttttaaa tgaaatagga agagaattta aagagagatt taaaggagaa
     6001 aaggtggata agatatttac aatagaggct tcaggaatag ctatagcatc aattgtttca
     6061 cagtattttg ataatgcacc tgtagtattt gcaaaaaaat cagaatctaa aaatttggat
     6121 aaagatgtat atgaaactaa tgtatattca tttactaagg caagagagta ttcagttaaa
     6181 gtttctaaaa agtacataaa caaaggcgaa aatatattga tagtggatga ttttttggca
     6241 aatggaagag ctgctcttgg acttaaagat ttaattgagc aagcagaagc taacttagtt
     6301 ggagtaggaa ttgtcattga aaaaggattt caagctggag gagcactttt aaaggctaat
     6361 gatgtaagat tagagtcttt ggcagtagtt gaatctatag ataatggaac tgtaaaattt
     6421 aggtaattta tgatattata tacttgtgtt tagcacatat tttaactatg taattatgga
     6481 ggatttttta tgagattttt tatagacaca gccaatattg aagaaataaa agaagcaaat
     6541 gatttaggtg taatctgtgg tgtaactact aatccgtctc taatagctaa agaaggtaga
     6601 gattttatag aggtagttaa agaaataagt gaaatagtag atggaccaat aagtgcagaa
     6661 gtaataagtt tagaacataa aggtatgata gaggaagcag aaaaattatc taagatacat
     6721 aaaaatatag ttataaagat acctatgaca gcagaaggtc taaaagcggt gaaagtacta
     6781 tcaagtaaag gtataaagac taatgtaacg cttatattct ctgctggaca agctctttta
     6841 gctgctagag ctggagctac atatgtaagt ccttttgttg gtagattgga tgatatatca
     6901 caaaatggtc ttgatttaat tgaagaaatc gttgatatat ttagtgtaaa tagtattgag
     6961 gctcaaataa tagttgctag tgttagaaat ccaatacatg ttttacaagc tgctagaatg
     7021 ggagcagata tagcaacagt tcctcttaaa gttataaacc aaatgattaa gcatcctttg
     7081 acagacaaag gaatagatag ctttatgaag gattgggaag gtgcttcttt aaaattataa
     7141 ttataaacta cgctttataa gtatattaaa gaggttatta tggaaattct aagtaataag
     7201 gctaggctat tttgcctagt cttattttgg tgtataatat attgaaacat attattgaaa
     7261 tagattaatt ttttaagtga ggaggcgaaa aatggataaa atattaagag agatacaaag
     7321 agaagataaa aaaaatccat atactgacca agaacttgct gaaatgttaa atgtagctag
     7381 gtctgaagtg atatcagtta ggaaaaaaaa taatatttta gattcgagag aaagaagaaa
     7441 aaaaatatta ataaaagata tatcaaaaat tgtaagtgaa aatcctcaaa tgtctgaaag
     7501 aaaaataact gaaatattaa tagaaaatgg atataatata tcgagaagtg cagtttcaaa
     7561 attattaaaa gaggaaaatt taaatcagtc aaagattggg attaaggata aagacccagc
     7621 atcatcagga aagtatgtga aggtgagtaa agttgtagaa gaagatggat ttgaagaatt
     7681 gataggtata aagggaagtt tgaaagaaaa ggtaaatcta gctaagtcag caataatgta
     7741 tcctcctaat ggccttcata ctataatata tggagaaaca ggagtaggta aaagtgaatt
     7801 agctacatgt atgtataaat atgctataaa aaacaacatt aaagaagaga acagcccatt
     7861 tatagtgttt aactgtgcag actatgcaga aaatcctaat ctattgatag ctcaactctt
     7921 tggggtagta aaaggtgcat atacaggagc agatgcaaat agagaaggtc ttgtagaaca
     7981 agctaataat ggaatattgt ttttagatga aattcataga ttgcctgctg ctgggcaaga
     8041 aatcttattt tcattaatag atagaggaga atttagaagg ttaggagaaa gtagttctaa
     8101 tagaaaggca aatgttctca taataagtgc tacaacagaa aatccagatt ctaatttact
     8161 tcaaacattt agaagacgta taccaatggt aataaattta cctaatatga gtgaaagacc
     8221 aaaatctgaa agatatgaaa taataataaa gttttttgaa agagaagcaa agcgagtaaa
     8281 caagaatttt ataataacta aagaagttat gtgtgcactt atgaattata agtgtactgg
     8341 aaatataggt cagttaaaga gtgatataca agtaacttgt gctagagctt ttagtaaatc
     8401 gatattttct agcgataaag taattataga tttagatagt ttaagggatt atataaaaag
     8461 tggatattat gattgtaatt gtaaaaatga aaagtattct gagttttcag ttgaggatat
     8521 atatatagat attagtgaat tggtgagtgg aaaaaataga attttatctg ataatgagat
     8581 aagcgagata tataattatg cagaaaaaga gcttaaggtt ttggaaagta agtgcttttc
     8641 agaagaagag ttaaaaaatg catttataga aaagttagat aagaagttta atgctattaa
     8701 aaacaataaa aatttatctg atagaagaag tcgaatagac tggggaggac aattaaaaga
     8761 gtctacactg gagataatgg ataaggtaat tgtatttatt aaggagcagt ttaagcatgt
     8821 aaatcaaggt ctgtacttgg ctctagcaat tcatattgag catgctatag gtaggataaa
     8881 agatggaaaa actataataa atccgagttt agataaaata aaagcatcaa tgccaatgga
     8941 atatgagttg tcaagatata taatgggtat agtagaagat ttgacacaag taacatttcc
     9001 agaagatgag cttggatatt tggcttatta tataaataaa ttttgttata atgaggaaag
     9061 tataaaagat aaagttaaag tagtaatagt tacgcatggt aaagttggaa ttgaaatgtc
     9121 taaagttgta aatcatattt taggtataga atgtacacta ggtatagaaa tagcacttac
     9181 agattcacca tcagagggga tagagcatgt attagaagaa cttcaaaaga tagaagctag
     9241 aaaaggtatt ctagttctaa ttgatatggg ttctttggta attcttggtg atgaggttga
     9301 aaaaaggctt ggaattagat gtaaaactgt aaatagagta gatacattat tggcaatgga
     9361 agcaggaaaa cttgcaacaa ttgaaggaaa aagtttagat ggtataatag ctgatttaaa
     9421 gaaaaataag aattatgcta tgataaatac aaataaattt agttacagaa aaaacgagta
     9481 tggaaagaaa aatgtaatca ttactttatg tttgagtggt gtaggtacag ctttaaattt
     9541 aaaagagcat atagaaaaac aaattaagga atatgacact ttaattgaag taaagccagt
     9601 tgctttttta aataataatt tagaagaaga gttaaatgat attgaatctg aatataatat
     9661 tattgctatc tgtggaacta ttgatataga ttataaaaac gtgcctttta tttcttataa
     9721 tgaggtactt ggtaaaaatg gtattaataa agtattagag catttaaata gtaaacaacc
     9781 agctttaaat attgaaaacc agttgaataa tttaatacat gaagatttga ttttatacaa
     9841 ttttgaagga atttcaaagg agtatataat tgatactctt gtttcaaaac ttgaagaagg
     9901 tggatatgtt gactcaaagt atatattaag tgtatataaa agagaagcaa tgggaagtgt
     9961 tgtaatggcc tctaaggtag ccgtgccaca tggacttcca gaaaacgtta tgaagcctgc
    10021 aatagcaatc gcgaggttga ataaacctat agtatgggac aataaattta tggtggattt
    10081 ggtggtacta ctggcactta aagaaaataa taagaaagaa atacgaagct tattttcaaa
    10141 gattaatgac cagcatactt tagaagtttt attaaatact gaagataaaa atgaaataaa
    10201 agaactttta agctaattat tagtaccgag aagaaagaca aaaaaatttg ctttagtata
    10261 aaatttaaaa ctactcaatt gctaagta
//
LOCUS       DAELTA010000132        10237 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.132, whole genome shotgun sequence.
ACCESSION   DAELTA010000132 DAELTA010000000
VERSION     DAELTA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.132"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(226..2106)
                     /locus_tag="KPG13_002817"
     CDS             complement(226..2106)
                     /locus_tag="KPG13_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG0986517.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2316..8279)
                     /locus_tag="KPG13_002818"
     CDS             complement(2316..8279)
                     /locus_tag="KPG13_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG0986518.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8403..8488)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8660..10063)
                     /locus_tag="KPG13_002819"
     CDS             complement(8660..10063)
                     /locus_tag="KPG13_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG0986519.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2825 a         1896 c         1156 g         4360 t
ORIGIN      
        1 aaaaattatt tttaacttct ttttaaacat attcaaaatt gaatttatat ttatatatat
       61 aaaagatatc tgctttattt aaaattaaac ttaatatagc tcaaattatt atacatttta
      121 acctcaatat tattagattg ttaagataat gaaaataaaa taagaatgca tttaaatata
      181 taactttata tctaaataca ttcttatttt ctatttactt aatatttatt tttcaattaa
      241 actcttaatc ttgtctatta cagatgaggc tattcctcca cctacttcaa acaactttga
      301 tgaattttta ccttttaatg acgattcttg tgatttatct aacgtatttg tagcaagtat
      361 aactggtgaa ttgtttttag ctgctaatgg tccaactgct aaagcatcta taagtttatc
      421 ttgatttgca actacaactc catctagctt agattgagag tagaatttat ctattacttt
      481 ggcattcgtc tcttgtctag tttgacctgc caccctattg tttgatatat ctttgtttgc
      541 tatttttcca acttgttcaa tagccttgtt agatattttt tctactccac caataaaata
      601 tatatttgac accttatttg atttaataaa ttgttcagct tcattatcta caccatcttt
      661 ttgtgtaagt actattggag ttttttcttt accagccagt gatgctattg ataatgaatc
      721 agcttctcca tttcctgctc ctatatacaa cttgcttaca ttattcgtct tatctatttc
      781 ttttgctata ttaatagaag tttgatatct tgtatctcca cctattcttt gtactgatat
      841 ttttgaattt acagctttga tagcttcaac aactgaattt ggcattgagt tgtttccacc
      901 tatcacaact acttttgatg gatttagcct tttcaattct tctacagttt tttgaggaag
      961 tttatcattt gatgataaaa gaatcggtga gttttttaca ctagcaagtg gtgtagctgt
     1021 aagcccatcc accatacttt tatcttctcc atttactatt acaactgtat ctgattttgt
     1081 ccaaccactt tgagatattt tgatagctgt ctcaaatcta tcatcacctt ttaatttttt
     1141 gactgtgaca ttagatgtag aatcacttga atttcctcct ccagagcttc caccactaga
     1201 tgagccgcct gaattacttc ctccaccatt atttccacca ctaccattat ctggtggtaa
     1261 ttcatccggt tttgataatt cattttcaac tatgtattct aaactttttg gaagtgtaac
     1321 ttcaacatat ttgtttattg taacttttct tcccttagtt gttttttgtg ctaatccaag
     1381 ttttaatatt acatttgaac cactttcttg tgtttcttta ctaacaatag aatagtttat
     1441 cctaatattg tttaataaat attcaagtgc ttcacttttc tctaccttat acttttcagg
     1501 gtcattttta ctagaaccag ttcccccaat cggttttact tcactctcat taataagggt
     1561 aaatattaat ccttcatcag agcttatatt ttcataatcc actgttttgt tgttatatgt
     1621 tacaattttt aaagctggtt tatcatttgt tccttttcct aaaactttat agtcagcatt
     1681 tgagttactt ccagattttt ttacagatat tcttcctata ttcaatctat ttccgtctag
     1741 taagtcttca tttgatgtaa catatatatc taatgtagtt gtattacttg atttgttgta
     1801 ttctgctcta caagtagcat ttgcatttcc actttttatc tgagatttta tattatcaga
     1861 ccaagtaatc ttttctttct gctcattatc tactacttca acctcacctt gtacttttat
     1921 actggcttga aatgaatata ctatgttttc tactgagtcc acagatatat ctatagtatt
     1981 tactttctca tacgtttttg ttccttgtaa aactggagct gcatatgcac atgtagcact
     2041 accagttaca gctactgcca tagttgatac tgctatagtt cttataaaag attttctttt
     2101 actcattaac acccgtcctt atctatcatt aactaagtgg ataaatttac ttatccacct
     2161 agcttttata tttaatttat aaattaccct aaatttatga ttacatttat atactttact
     2221 tttaatatct actattttaa aactattcta gtacctaatc ttattagata tacaatgtta
     2281 gtgttagtat ttttacgttt ttagagttcc acctactatt tatctctaac aatattaccc
     2341 tttactttat tgcttacagt tataaccttc ttattactga tatcaataga atttcttgta
     2401 gcttttctag ctttactatt actatcagta agacttattg tctttaactc atcataattc
     2461 ttagctccta caggtacata cattgtatca cttacaaatc tttgacctac tggagtatct
     2521 ccttgaaggt cattgtatct atatagttca gccttaacct tatcacttaa agaatcaaag
     2581 ttttcttttg gcatatagta aacccatgta cctttactga tttcacctag ttcacctttt
     2641 tctggaactt ctgcaaataa aagtgcttct ccagatatag tcttattgtt tttatctata
     2701 agtagtggta aattaaatgc tggatttcca ctgtattcac ctgtaataac tattgaaccc
     2761 gctggaatct tacctgtttc atcttcactt gtgctagagt catcactttg atatacataa
     2821 tcttctgcta attttccaat tccattagta cgagtttcgt ctgcattatt cattgtgcca
     2881 atttctatat tatcacctgg aggtcctatt atatctaatt ctgcaacttc tatattagac
     2941 ttattatttg agattagttt tacatatcta gcttggtatg tccaaagttt accatcttta
     3001 ttaaatgtaa ctctagcttc attatcttgg tcttttcctc caccaactaa atcttgtcca
     3061 tattcaaaag ttccttgact tacatcttcc cagttctttt tatcattact tacttgtatt
     3121 ttgtaattag ttaaaggatt gtatgttgtg ctagtattct ttctaaagtt aaataatcct
     3181 tttaatgaga acttcctagc ttctgtctct ggttttgtat attttagtcc tactataggt
     3241 aatgtttctt tcatgtctac tattatatat ggattttcct ctacatttat atcagggtct
     3301 ttttgagggt ctttgttcca ctctgcttta cttattatct ttcctttata tgtagtattt
     3361 gcatcattat cttttatatt atcaatttca ggattttgta atggtccatg tgagtcattt
     3421 tcagttctaa catcaccgtc agaaacagta tttgttatga atgaccatgc tgatttattt
     3481 attttccctt catctgataa ttttacagta ccaacttcaa attcttctgt tggatttagg
     3541 tgataatcat atgcaactac cttatatgta aatgctctgt tattaagagg agatatatta
     3601 tcagtaaatt tagtcttact atttcctttt gcatccacaa atccaactgt tctgtatttt
     3661 acctgtgttc cattttcacc atctaaataa tttccatcac ttcttattat ttcgtatcct
     3721 agtatcttat ctttatcctt atctacatct aggtctatag atattttatt agatttaact
     3781 agagaaccac tttgtattcc atcagaaaaa ctagcactta ctttagtatc ttttgccata
     3841 ctagctatat tttcatttaa aatctttctt ctagcttcgt catttacata ttgtatcttt
     3901 cttgattctt tttcgtagtt cttactatcc attatactgt ttaattctgg tgtaatttct
     3961 actccccatg ccttaaagta atctcttaaa tctttttcaa caacacttga tgccattgct
     4021 actagcattt ggtcttttgg caaactatta attgggtcac tacttgaaag tgctctgtat
     4081 tttcttgaaa tctttgcata gaatgtatca ttacttaaat cagcatctga gttattcttt
     4141 aacattgaac ccgtaaaatc atcatcataa gctagatgta attgccataa cattccaagc
     4201 ctttcaaata cattgcttgg tatacctaag ctatttgaag ttacatgctc atatatctta
     4261 tccattgttg aaccttcaat tcttgaatgt gtcttatcat caaaggtctg agcaagtaaa
     4321 gcaagtacat tgttactagt ttcgctatat gtcatctttc caatatctgt aacatgtcct
     4381 atctcatgac ctattcccca tccaaataag tttcctcctg tagcttttcc actatcatct
     4441 atttggaaag gtactccatt cataagccct ggaactgaac catattctac tcctacatgt
     4501 aatccagaag catacataaa tgctccaata aacattcttt ggtattttat gttcatacga
     4561 gattttggag ccatatcatt tttagttaag ttaaattcat tatcatctac agttccatta
     4621 ccatctaaat cttgtgtttt aaatactcct ctttctgagt aaactaaatc cattatttgt
     4681 tcccatgcaa gcatactatt atataatcta tctacttgtg tatttaaatc agcatttcct
     4741 tttgatatcc cttgatatac agctgttgca ggaacattta atgttacttt atcactttca
     4801 atatctgtag tgtttaacac accagttttt tctgaatatc catatgaatt accaacattt
     4861 gttcctggat aatatggttc tccttttaca gcatctttat atctagctaa ttcttctata
     4921 tatgctttta tctttttctt agattcagaa actcccgcag gactcttatc atttattgtt
     4981 ccatttagat taaggtgtgg tatcttagta gctcctgcta cacgtacttt tatatctcct
     5041 ccacttggag tatcttcagc gtatcttatg tataaacttc ctccacattc tatatctaaa
     5101 ctatgaagtt tgcttaattg aattacattt tttccagtgt taagatttat ttctccactc
     5161 ttatatgcac cagattctcc atagtactgt gtatatatca acttaggagt ttttccactt
     5221 ttacttccaa catatactgt gatttcatct cctgctctag cagctactcc taatgcctgc
     5281 cagtcatttt gcatacctaa attattggaa tttacagtat actgtccgtt tttatcttta
     5341 tattttccta atttatttat ttcttgattt acagtagtta catcagtaag tgtggctcta
     5401 tcattatata agtcttgagc taattttaat tctctctcaa taactgtctt atatggatga
     5461 tattctccac tagctttatc tggagtattt aatctatctt ttaattcagt tattttttct
     5521 tgagttacat cttcttttaa ttcaacttgt aggtcatcca taaatagatt tcttacatca
     5581 tcttctatac tgtcatactc ataaaactta agttctgata tattagactt atatgaatag
     5641 gatgaacaaa cacttacttg tatcttggta gcagttattg gttcttttaa tctaaccatt
     5701 acatacttat tattatttac agggtctgtc tttgtttcaa aaggaacatc ccccagattg
     5761 tgtaattgac cattttcatc ccaatatcgt acctcaaagt catttattgt cttatcagaa
     5821 acatcaaaac gaggtgtagc cataaaagta tccatagtat aagatttatc aaaagttatt
     5881 attggtcctc tattcgttcc ctcagctgca ttatatgttg ggaatgtcca atgtgttgta
     5941 aagtccccat ctactacatt gaatttatca aatggatttt tatcactata ttcacttttg
     6001 tcatatcctg ctggataagt aacatctaca atgtgatttg ttggtattcc aaatccacca
     6061 ttctctttag gagtattaat taatctatag ttaggtgttg ttggtgcatc catgctttca
     6121 gttgtaccta tatatgtttt agacattcca ctagttccca agtggttagt agctgtcatt
     6181 ctaacttcat atgttgtatt atccttaaga ccttctatct ttgaacttgt cccacgaata
     6241 agcttatcat tgtattttat ccaagaccca ttagagcctt tttctttata gtacaagtca
     6301 aaatctttag cttgtttatg atttttccag cttacatcaa gagatttaaa tttaccttta
     6361 acatttatac cttctggtgg ttctggtttt gtgtctggaa caacttgaaa ttctaatata
     6421 ctatctgggc taaattgtat aggtttatag tttgaatcta cattttctgg tactccgtct
     6481 aagttttctg gtgttgcatc tttataacca ctagaccaat ctccatttaa agattgtatg
     6541 ctaaccctat atagtccata tgcttctata tctctaactt ctattgtatt tttactggtt
     6601 tgtaattttt tagtagataa tacttttccc ttactatcaa ttttatctag tataagttcg
     6661 tatgctgata catttacttc attattccaa taaaaagata ctttttcatt tccaacagca
     6721 gtgctagtcg atattttatt tattttaggt atattcatct ctggctttgg tatttcttta
     6781 tatacattgt ttaaaaattc aacttttgat atttctgcaa gatttctatc gtttgctgtt
     6841 gtagctgtta tttttatagt tatctttttt actgctactt gtttaccaag atttattact
     6901 atatcactat tttgaacatc tgcatatgtt tctaatgttg acttactata tgtagatgtt
     6961 gtagagttat catctttact gatattaccc tcttcttttg gagtatcttc atctgaatta
     7021 ggtttatcca ttgatgaatt ttctttatca ttatttttat cttgattggt attagagttt
     7081 acttcttgtt tatcgctatt ttcctgttgt tcatcattat tttcatcttt attatttgag
     7141 ttattatcag tctggtttat attttcatct ttattattta cttcttcttg atttacttct
     7201 tcttgttcgt tatttacttc ctcttgttgc tcctcatctt ttaagttttt catatattct
     7261 ttgttttcat aaggaatctg ttgaacatta ccatctttgt cagtaacctc aactattcct
     7321 tttgctggag cagtaccttc tggagctttt atattgatga cttccatatt aagtgcttct
     7381 gataaagcta tatccattac tgctggtttt tcctcagata tttcatcatt ttttgaagat
     7441 attgtaactc cattattttc tccactaaat aaattttcta cattaccatt aatatctaca
     7501 tcactatttc cttttaattc aaccttactt gtatctacta taggattggt agatactata
     7561 ctttctaggc tttttgcatc ttcactttgt cctaaatttt tctgtacata atctaagtca
     7621 acaatatcta cctttccatc tctatttaaa tcaaattctc ttttatttga atctatattc
     7681 tctaaaacct tattataatc actcttatct actttaccgt ctccatttac atcacccatt
     7741 aagaacaatt cataatttaa aggtttattt ttattatcta cacttgctct attgcttaat
     7801 aagactcttt gtgaataact tgatatatca atgtctttaa tttctttgtt cttaaatcca
     7861 tctccagaga cttctacatt gtattttcca acaggtaaat tattaaatac aatattataa
     7921 tatgatatat tctcatcttt attttttatt tcacttctac ttgcagataa tttttttata
     7981 gtatattcta tattatctgt cccaacattt actgttcctg aatctttttc tccaagcttt
     8041 attattccac tattgttact atcatctttt aatcgtaact gcatattagt catactttct
     8101 gaatcgctat tttttattgg cagtgaaaaa tttaaatcta cttcaagact cccccttaac
     8161 ttttctgtaa ctgatttttc aactgccctt agcttcccct ctaatatagg ttctgcaaaa
     8221 acgtttagtg gtgcactaca catagatact gctagtacac aagatattgc ctttttcatg
     8281 aaataacaat cctcccttaa taaatatcta atttttcatg aatttaaata ctataagttt
     8341 cagacaataa aaaaagacac tcaaagagaa tcttaattat aaaataaaaa gactttactc
     8401 tttttagcaa ctggctgata tggctttaaa agctttaatc cctatagttt tgcgtcatta
     8461 gatttcccta attttgccat ttaattttca ctactttctt acccatttta tactatattt
     8521 aaacttttta atacaatttt tttagattaa attataaaaa aataacattt atattaattt
     8581 attaatataa atgttatttt ccattataat ttaattattt atatagcttc cttacaagca
     8641 taattttgtg tatattttct tattctttat atcttccata aaattcatgt gtagcatata
     8701 gattcttatc tcctgttata gctataccaa atcccattat ttcataccct ttatgcaaca
     8761 tgttagttcc ttcttctgga tttgtcttcc actcattaaa aataaagttt cctatatcat
     8821 ctgcatctct atttgtatat acaggtttat ctggaatttt atatttaatt atattttcac
     8881 cttgaactgc tatatatcca ggtttaatct cactccaatc taagtatttc aaaaaagtag
     8941 aatatgaatt ttttccattt atcgtatgag ataaagtctt cttatttgcc attaaattag
     9001 accaatcatt tgctaaaccc tctaatctgc ttgctacaga taacgctctt actccattct
     9061 cttttctata ctgctttgta agagcatata ctttaccctt tattctctct ttaaatgctc
     9121 tatcatttgg attgtaatat aaaccttgtt gatttggtga agcttgattt atacattgct
     9181 tgataacatt ctgatttatg cctcctagta cagtcaattt gtctgcacca cttaaaatac
     9241 tcttgttact gccattttca actaaaacta caggagcatt ttttgctatt gtagaggcag
     9301 ccaaagcgtc tgttaattgt aagcctttac ttagataaaa ttctttacta tttttataaa
     9361 actcttgtat aactatctta ttagtttcaa atctatctgt gccacccaat ctaatagaat
     9421 ttgtgctctt aactaattcg tcgctgatta tttcactaga accaagcgca taactcttta
     9481 agcctgtcgt gtcaaatccc acactatttc catctgtaag tattataggt actccatctc
     9541 tagcagctac tggtgatgca cttatcgcat ctgcttctcc tgtaaatcca ttggctataa
     9601 ttactttatc tattggctta atttctttga ttttttcagc tatttttata cttgtatcaa
     9661 atctattttc accaaagatt ctttgtacaa ccttcttttt ggatagtaac tgattctcaa
     9721 catttttact tattgttgat tctgagccta ttatatacac agtctttgct ttgtttaatc
     9781 tttccatagt tgtgtttgga atctgatttt gttttgtcat caaaataggc gcatttaagg
     9841 ctccagctaa accacttgca cttagaccat ctgctaagct attatctgag ttgattagta
     9901 ttgctgtatt gtagttttgc ttatctgcta tttttgctgc tgtctcatat ctgtcattcc
     9961 cttttatttg ctcaactgat gtcaacgcat ttaccatagg agtatttacc attactaaaa
    10021 atagtgtaag tcctatagct aatatctttt tactattctt catattattt tctaccccca
    10081 atgtactttt catatttttt attagtactc atacttattt ttaatcaaaa gttacaattt
    10141 tgttaccata ttttatatat gataccatag ttatccatac aactcaaaca atatacctaa
    10201 agtttttatt ctctataaat tcaaaacatt ataattt
//
LOCUS       DAELTA010000133        10237 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.133, whole genome shotgun sequence.
ACCESSION   DAELTA010000133 DAELTA010000000
VERSION     DAELTA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.133"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            140..1045
                     /locus_tag="KPG13_002820"
     CDS             140..1045
                     /locus_tag="KPG13_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0986520.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1169..2122
                     /gene="msrA"
                     /locus_tag="KPG13_002821"
     CDS             1169..2122
                     /gene="msrA"
                     /locus_tag="KPG13_002821"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG0986521.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            2275..3405
                     /locus_tag="KPG13_002822"
     CDS             2275..3405
                     /locus_tag="KPG13_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986522.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(3522..4490)
                     /locus_tag="KPG13_002823"
     CDS             complement(3522..4490)
                     /locus_tag="KPG13_002823"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG0986523.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            4984..5187
                     /locus_tag="KPG13_002824"
     CDS             4984..5187
                     /locus_tag="KPG13_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986524.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            5351..6505
                     /locus_tag="KPG13_002825"
     CDS             5351..6505
                     /locus_tag="KPG13_002825"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG0986525.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            6518..7522
                     /gene="rseP"
                     /locus_tag="KPG13_002826"
     CDS             6518..7522
                     /gene="rseP"
                     /locus_tag="KPG13_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG0986526.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            7705..8760
                     /gene="ispG"
                     /locus_tag="KPG13_002827"
                     /gene_synonym="gcpE"
     CDS             7705..8760
                     /gene="ispG"
                     /locus_tag="KPG13_002827"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG0986527.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            9108..9932
                     /locus_tag="KPG13_002828"
     CDS             9108..9932
                     /locus_tag="KPG13_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986528.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         3977 a         1093 c         1794 g         3373 t
ORIGIN      
        1 gtattaaagg tttgaatgta acttaaatat taaaagagga acattgtaat atgtaacttg
       61 cataaaatat atatactata gtgaaattat tttgattaaa atttaaaata taaggtgttt
      121 tatgtggggg tatatgtata tgaaaaattc taattttaat atattggcat ttgatggtgg
      181 tggattaaaa ggggcattga gtatatccat acttgagagg atagtaaaag agtatccaaa
      241 tctattaaac aatataaata tgtttggagg tacatctaca gggagtctaa ttgcactagg
      301 gcttgcgtat ggtgtaagcc ctaaagagat aaaagaactt tactcaattg aaaattcaaa
      361 gtatattttt aataaaagtt atgcagaaat actcagacca aagtatgaaa acaaaaactt
      421 aaaagaagtt ttattaagta tatttccaga ggaactagaa ttaaaagatt taaataaatt
      481 agtaatgata ccaagttttt atataggaaa tgaggaaaat gcatggaaac cagtgtttta
      541 taacaatatg ccaaattctt ttacaaagac tagcaaagtt gtagatgtag caatggcaag
      601 tagtgctgct ccggtatttt ttccaacata taatcgtcat gttgatggag gaataatagc
      661 aactgacccg agtttagcgt gtataattca tgccattgac agtggattta aacttaagaa
      721 tacaagactt ttttcaatag ggactggata tgtatataac agtataaagg cagatactac
      781 cgaatgggga gctattgact ggataattaa taaagaaccc gatttaccaa ttatatctat
      841 taccttagaa ggaaattctc aaatgagtca gttattttct caaaaacttc ttggagataa
      901 ttactataga ttaaatccta agatggaaaa agatgtagct atggatgatt gtgatgcatt
      961 agaatatcta atgtctttag gagaaaattg tgatataaaa gatgcttttg gatggataaa
     1021 gaaaaagagc ttttatagaa attagattca atttgcaatt gtatttttac acatgatttc
     1081 attgagtagg taaaaataat aaaatgcaga ttattaagtt acttcttagg gaataaataa
     1141 agtataattt atttaaggag tgatgaaaat gataaaactt gctacttttt caggtggatg
     1201 tttttggtgt atggtaaaac cttttgataa gtatgatgga gttattaaag tagtttctgg
     1261 atatacaggt ggtcatgttg aaaaccctac atatcaagag gtatgctcag gaactactgg
     1321 tcatttagaa gcaattcaaa taacatatga tgatgatata ataagttatg atgaacttct
     1381 aaatatcttc tggaaacaga ttaatcctac agatgatggt ggacaatttg gtgatagggg
     1441 aactcaatat aagactgcta tattttatca tgatgaagaa caaaaaaata tagctttaga
     1501 gtcaagagaa catctggaaa atagtaaaat atttgatagt aaaatagtga ctgatataat
     1561 tccagcaact aaattctacg aagcagaaga aaatcatcag gattattata agaaaagacc
     1621 attaaactac aatatgtatt ataaagggtc tggaagatat aattttgtaa aaagccattg
     1681 ggatggaaac aatataaata gggaagagtt aaaaaagact ctaactccta tacaatttga
     1741 agtcactcaa aatgatatga cagaagtacc ttttgaaaat gaatattatg acaataacaa
     1801 agagggaatt tatgttgata ttgtaagtgg agaagtgtta ttttcttcaa aagataaatt
     1861 tgattctgga tgtgggtggc caagtttttc aaagccagtt atagataaat ctatcattga
     1921 gagaagtgat ttctcacaag gtatgtacag agttgaagta agaagtgcta aaggaaattc
     1981 tcatttaggt catgtatttg atgatggtcc aaaagaactt ggaggtttaa gatattgtat
     2041 aaattctgct tctcttaaat ttataccaag agaaaatatg gaagctttag gatatggaga
     2101 atatttaaaa ctttttgagt aattaggaat atttattata aacttatcta aaaggtgaaa
     2161 atataaaaaa atattttcac cttttgtttt tttgtcaata atatgataaa ttattaattg
     2221 taatcttatt tataccaata gattaagaat ttaattttga aagagagaaa acaaatgaat
     2281 ttaaaagact ttaatggaaa gagattaaaa tcagctagaa tttttagagc aaagacgata
     2341 gaacagttgt ctaaagaaac taaaataaat aaaaaagatt taaaagcttt tgaggaaaat
     2401 aagtatgtac caaatataga aaatacatta aaattatcta atatattaaa ttttccaaaa
     2461 gagtattttt ataaaaatga aaatataaat atcgttgtag aggatagcca ctttaaccct
     2521 caatctaaac tacctagagt agaagagatt tcttatagag aaaaaataat tataatacat
     2581 aaaatatatt catttatgga gaattatatt aagtttccaa aagaaaactt gccaaataga
     2641 aaagtaatgt cagatattaa tataaatgat atagaatctc ttgcatataa gactagagag
     2701 ttttgggatt taaatagtac tccaatagta aatatggtaa gtttgttaga gtctaaaggg
     2761 attataatat caggtatgaa tgtagataga aaaggggcaa ctatctttac tcaaaaacag
     2821 agaattagta aagaatctaa atatctaata tctctgggta atgacaaaaa gtcagcatca
     2881 attagaaatt atactttggc atgtgagctt ggatatataa tatcaagtga attaagaata
     2941 ccaagtaagc aattttcaga agatgagtat gcctgtgcat ttttattgcc aaaagagagt
     3001 tttttaaaag atttgactca tccagaagat ttagattatt atgtagaact aaagaaaaaa
     3061 tggattgtac ctatatctgc tatgattttt agagcatata atctagaaaa gataaattac
     3121 aaaaaataca attatcttat gaatgaaatg gataaaaaag gctggttgat agaagaacca
     3181 ctagacaaaa tgaaaggttc aagcccaacg tatttaaaaa gagcagttga attattgata
     3241 gaaaataaaa taatgagtgt aaatagtata gtatcaagtt tagaggaatt tggaataaat
     3301 ctttatccag aagacttaga attattgatg ggattgaaaa aaggattgtt atctcaagag
     3361 gtaaataaaa aaagtaaagt tataaaattt gatggtaaaa aataggttta tattttagta
     3421 ttaaaattaa aagcaggtac aaatgattta aacattaact tagttaaagg tcttttcaat
     3481 aagtcattta cctgctttta gtgtaagaaa gaatattgaa tctaaagtgt ttttgaaagt
     3541 gcaatatatt cccttaaaac agaacaagat ttattaaatt cattttgctc atattcagtc
     3601 atattgattt caataatttc ttcaatacca tcttttccta taacacatgg aacacctgca
     3661 aatacatttc tttcgttata ttgaccttct agtaatgtag aaactggaag aacttttttc
     3721 tcattgtgta gtatagcttt tacaatttct actaaagcag tacctatacc aaattctgta
     3781 caatctttac catttattat acttatccct gtttttttat ttttttcaac tattgatggt
     3841 aagtctaaat tgctataagt acttgggttg tcttttatca tgtctaagat tggtttacca
     3901 ccaattgaaa catgagacca aggaaccatt tgagaatctc catgttcacc catagaataa
     3961 gcttgaattg attgttgagc gattccagtt tcttcagata aaatccttct aagtcttgtt
     4021 gaatctaaag ctgttccagt tccaatgact ttgtttttag gaaagccaga tttttcccaa
     4081 atataatttg taactacatc aacaggatta gatatgacta caaatatacc atcaaagcca
     4141 gaatcaacta tgggttttac tatagatttt atcattccaa tagttggtct tagtaaatct
     4201 aatctagttg tgttttgtct acgtaaaggc ccttctgaag agtctgcaac acttatcact
     4261 actatgtcag aatctttaca gtcagaaaaa gttccttttt ctatatgtac tttatgtggc
     4321 agatatgaaa ctgcatcagc taaatctaaa gcttgagctt ttgatttaga ttcatctata
     4381 tcaatcataa agagttcatc acaaactcct tgagttatta aactaaatcc acaatgagaa
     4441 ccaacatgcc ctgaacctac aatagatatt tttcttggct ttattgccat tttaaatcac
     4501 tccccctaaa ataacgatac attgaaaatt atagatgtta ttaaaagctg tttttggata
     4561 ttttaatatt ctattatttc agaaaattta tttaatatga ttttatcaag aataaaagat
     4621 taaactaatt ttaattattt taattgttca ttctaaaata gtatagttct aactttctat
     4681 agtatccatc atagcccttg tctcttatta taatcaagta ctttaaatat ataataaaat
     4741 ctttcttata tatctgcaaa tataataata gtatattact ttaatttagc accaataaaa
     4801 atttttatat gttaataata atgcatcata tgtgttatat accaatcatt tagtatagta
     4861 ttaaattttc atattagaag gataaaatta tttgacactt aagcaggtga ttatttatct
     4921 aaatagatga aaattttcca aaatagataa aatgtgatag aatatatttt gcgaggtgat
     4981 aacatgattt catacgctcc attatggaaa ttgttaattg acagaaaaat aaagaaaatg
     5041 gaatttgtaa atatatcagg tataagtatt tctgttttag gcagactagg aaatgataaa
     5101 tctgtttcta tggatacaat ggagaagata tgtttagcac tagactgtaa aatagaagat
     5161 gttgtggaaa taaaaaaggg catttaataa tactagaata ttttataata ttccacaaaa
     5221 tacaaaaaat aatagattaa ttttaatctg tggaataata tatgaaattt ctatgaatag
     5281 taaaaataat tgttttagta gtgttataat gttttgacag ggtatattta ttattagaaa
     5341 ggattattat atgaaaaaga tatcaatttt aggttctaca ggctctatag gtaaacaaac
     5401 tttggatgtg gttagagaac atagagataa gtttgagata gttgcaatat cagctaacag
     5461 taatattgaa ttattgctag aacagatagt agagtttaaa cctaaatatg taacagtttt
     5521 tgaggaaaat aaagcattaa aattaaaaga aatgttgcct aaaaatatag aaatagaagt
     5581 tttagcagga atggaaggat taaaaataat atcatcactt gatgaagtgg atgtcctttt
     5641 aactgctgta gtaggtatga taggattagt cccaactctt tgtgctataa agaagggaat
     5701 agacatagca ttagcgaata aagaaacgtt ggtaactgct ggtgaacttg taatgaaaga
     5761 agcagaaaaa tacaatgtaa atattcttcc tgttgatagt gaacatagtg ctatatttca
     5821 atgtctaaat ggagaaaata aaaaaaatat agaaaagata atacttacag catctggtgg
     5881 tccatttaga ggaaagaaaa agggagaact tgcaaatata actaaaaatg aagctttgaa
     5941 gcatccaaat tggagtatgg gaagaaaaat aagtattgat tcttcaacgc ttatgaataa
     6001 aggacttgaa gtgatagaag ctagatggtt atttggagta gaacaagaaa atatagatgt
     6061 agtagttcac ccacaaagca taattcattc aatggtgcag tatacagata gctcaataat
     6121 tgctcaatta ggatgtccag atatgagact accaatacag tatgctctta catatccaga
     6181 tagaatggaa agtagctttg aaagaatgaa tttttcaaaa tttagtactt taacttttga
     6241 agaaccagat ttagaaactt ttccatgttt aaaattggca tatgagtgtt taaaaatggg
     6301 aggaacttat tcttctgttt taaattcagc aaatgaagtt ttagtaagtg aatttttaga
     6361 ggataaaatt ggtttctatg acataccata ttacatagag aagactttag aagttcatag
     6421 tagcataagt gaaccaacat tagaagaaat cttagaaaca gatagatgga gtagagctta
     6481 tgttgcaaat ctgataaaaa aataggaagg tgaatttatg actataatag ccgcattaat
     6541 actatttagt ataattgtat taatacatga gttaggacat tttatatttg caaaaagaag
     6601 tggtatcaaa gtaaatgagt tttctatagg tatggggcct aagatatata gtgtaaaaaa
     6661 agatacagag tattctataa gagcacttcc aattggtgga tatgtcagca tggaaggtga
     6721 agatgaagaa caaataagtc caaattcttt tggaaacaag tctatattac aaagatttag
     6781 tactattgta gcaggaccaa tattcaatat aatattagca gcaatacttt tagttccagt
     6841 atttttgtat ataggttctc caacgactaa attaggaaag ataatgccag atactcctgc
     6901 acaggctgta gggcttcaag taggagataa gataaataaa attaatggta attctgttaa
     6961 gacttgggat gaagttgcaa atataataaa cacttcttct ggtggagaat taaaacttag
     7021 tataactaga gatggaagtg ataaagttgt aaatgtaaca cctaaaaata acaatggaaa
     7081 gtatgaaata ggaattcaac cacaaagaga aaaagacttt ttagggtcaa tagtaaatgc
     7141 atgtaaaact actgtagata tgacaaaaca gatgctaaca tttctaggtc aaatgattac
     7201 aggacgtgta ccaggaggga ttggcaatgc tgtagcagga ccagtaggtg ttattggtat
     7261 ggtatcagat gctgctcgta caggcatcat aaatgtagtg tatttagcag ctgtcataag
     7321 cttaaattta ggtatagtaa atctattacc aataccagca cttgatggat ggagaatact
     7381 tatgttgtta ctagaagctg ttagaggtgg taaaaaactc gacccaaata aagaaggcat
     7441 gataaatgtt gttggttttg gagctttaat gttatttatg ctttttatca catataaaga
     7501 tatactaaga ttatttcaat gatttagttt tttagagatt ttaagaaaat aggctatgta
     7561 gttttaaata agaataaatt aatttttata tatgaaattt attcttattt aactagatag
     7621 ctttttaatt tataatatac atgtaggtta taatatttta atatttaaag agattcaata
     7681 taaaataata tatagaggtg gaatatgagt tacgagagaa gaaagacgag agaagtaagt
     7741 gtaggagctg taaaaatagg aggagaaaat cctataagta tacaatctat gacaaatact
     7801 gatacaagag atgcagatgc tacaatagct caaataaaga gattagagga agctggttgt
     7861 gatatagtta gagttgctgt tcctgatatt aaagctgcta aaaatatagc aaagataaag
     7921 tctagtgtaa atataccaat tattgcagat atacatttcg attataagct agcccttgaa
     7981 gctatagaac aaggtgtgga tggaataaga ataaatcctg gtaatattgg aagtatagag
     8041 agagttaaga tggtagttga gaaatgcaag gaaagaaatt taaaaattag aattggagtt
     8101 aatggaggtt ctttagagaa agaactttta aaaaagtatg gctctgcaac tgctgaagca
     8161 ttagttgaaa gtgcaatggg acatattaag attcttgaag acttagattt tcataatata
     8221 gttatatctc taaaatcatc cgatatttat aaaactgttg atgcatatga attaatatca
     8281 aaaaaagttg attatcctct tcatattgga attacagaat ctggaagtgt tcacaaagga
     8341 acaataaagt cttctatagg ggtaggagca cttcttctta aaggaattgg agatactgtt
     8401 agaatatctt taacaggcga ccctgttgaa gaggttattg ttggtaaaca gattttaaga
     8461 agtttgggac ttttaaatga taagattaaa gttatatcat gccctacatg tggtagatgt
     8521 aatatagact taattagtgt tgtaaatgag gttgaagaaa agattggtag tatggaaaaa
     8581 aatatcactg ttgcaattat gggctgtgct gtaaatggac ctggtgaagc cagagaagca
     8641 gatataggta ttgctggggg taaaggtgaa ggtcttttat ttaaaaaggg tgaaatagtt
     8701 agaatgattg atggtaacaa attagttgat gagttgctag aagagattga aaaattatag
     8761 tatacaattc atatatatat taaatgacta gatttttaaa tacttttgta ataagttttt
     8821 atttacagta tttaattatc tagtcatttt ttattttata aacttcatta aatgtaagaa
     8881 acttatattc taatttgact atatatgtat gagttaaatc atattgtaaa tttataaaat
     8941 taaaaaaaga tttaatatag tgaaaatttc catcaatagt attgaaactt gatagtaact
     9001 atgataggat atagttacta gaacagttgg taaattatgt taacatagat tttaatgttt
     9061 tggtaaattg ttttattata agttaataga aaaagaggtg aagaattgtg agatataaaa
     9121 aaatatctag cttacctatt ttagttgttg ctttgctgat tactggaacg acaacaacat
     9181 tttctcttag tacgaccaat tcagaaaaaa caaatgtata cttagcaaat acggtaagta
     9241 ctgtcacaaa gaaggtatca gatattcaaa gtagtaaagg tcttgtttat aatgagaata
     9301 ttccattatt tataaataat agcaaaatac agtatgatga tactccatat cattggccta
     9361 gcaatgttat aagcttaaca aatagcagtg aaaaggcaat tatggattat gaaattacat
     9421 gcttagcata tgataaaaat ggaaaaccac ttgagctata ttgggatgct caaaatgtgg
     9481 cagcagatgg cgaagtagga agtgttggat ttagcccagc tggtgtagac tatggtattg
     9541 ttacaggcat atctcctgtg tctccaaaat catattcaca tacttataga aaaatgcaac
     9601 aatcaccacc acaggatata ataagtatgt ttgaaaaaca acaaggcaag gcatgggtag
     9661 aaaattggtt gaaagaatgg aaacaaatgg agaaagaata tgcaaagcaa aatgcaatag
     9721 caccaggtaa gaatcaaaat gatgcctttc tattgtttga taaatggaaa caatcaactg
     9781 gagagcatgg tgtaaagtac attatatctt gtgtaaaaca agtaacattt aatgatggta
     9841 gtgtatggaa aaactcagca tatgagaatt ggttgaaaag ttttcaaggg aaggaagttt
     9901 caaatagtgt attagaaaat tactataagt aatgtttaat gatagcaaaa tgagtacgaa
     9961 tttaattaat gttaatttaa ttaaataagg actattttgt ttaaaaatat cagtgttaca
    10021 agtaaatttt tattgtgaca aaatgatata tatacataag attataagca tgtgtcaagt
    10081 tttgatacat gcttttttat tttgaatata tgttttctat aaatttatga ttttttagtt
    10141 atgtaatttt atatgtgaat ttttagattt ttatatagat agtgaaataa tgtggaagtt
    10201 ttatgtttta aaaattttag ttaaaacatt tattaaa
//
LOCUS       DAELTA010000134         9933 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.134, whole genome shotgun sequence.
ACCESSION   DAELTA010000134 DAELTA010000000
VERSION     DAELTA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.134"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(174..326)
                     /locus_tag="KPG13_002829"
     CDS             complement(174..326)
                     /locus_tag="KPG13_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986529.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     gene            complement(476..1903)
                     /locus_tag="KPG13_002830"
     CDS             complement(476..1903)
                     /locus_tag="KPG13_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG0986530.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            2094..2915
                     /locus_tag="KPG13_002831"
     CDS             2094..2915
                     /locus_tag="KPG13_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG0986531.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            3040..4557
                     /locus_tag="KPG13_002832"
     CDS             3040..4557
                     /locus_tag="KPG13_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0986532.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            4580..4864
                     /locus_tag="KPG13_002833"
     CDS             4580..4864
                     /locus_tag="KPG13_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0986533.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(4961..6631)
                     /locus_tag="KPG13_002834"
     CDS             complement(4961..6631)
                     /locus_tag="KPG13_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG0986534.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(6703..7950)
                     /locus_tag="KPG13_002835"
     CDS             complement(6703..7950)
                     /locus_tag="KPG13_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG0986535.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(7937..8611)
                     /locus_tag="KPG13_002836"
     CDS             complement(7937..8611)
                     /locus_tag="KPG13_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG0986536.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(8783..9862)
                     /gene="mltG"
                     /locus_tag="KPG13_002837"
     CDS             complement(8783..9862)
                     /gene="mltG"
                     /locus_tag="KPG13_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG0986537.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         3392 a         1585 c         1189 g         3767 t
ORIGIN      
        1 gatatttaat aagttaatag ctctataata aaatttttat cattgtttat aaaaaatttt
       61 actttgcatt tttttataaa caacagtttt atttataatg ttttgtggta taataaaagc
      121 aagaagaact acaatctatt ttgcgttaga gtggagttca tcataaacag agtttatttt
      181 ttggatttaa atataaattt aaattcaact cgtaagtcac tctttgtgcg agagtggctt
      241 tttgcttttt tgaatagttt actaattaag taaactacca cactagctgt taaactagct
      301 aaaacattaa acaaaaagtt atccatgaac tcacctccct tcgaatcgtt gggaggataa
      361 tcttttgtac atgaactcca ctctatagat tgtaagttaa atcttcttgc taaaaatatt
      421 ataacatatt atggtaaatt atataattaa aaacacttgt ttaaaagttc taattttaat
      481 tataaacttt cccagtttct atatctataa aaaaccaacc aaaagtacca ttactatttt
      541 ttgaattgat tttaacagca tagacattac cactatatat atcatctcta aaatctatca
      601 tatcaactac taagttattg atactgactt tatgtctctt agcacattct tttaatgcta
      661 tctgttttgc ttgttcctct gttatacttc ctttattttt ataatcatct ggattacata
      721 ctttcatatt accattaaca tcatatgtat aaactttgct catatcattt ttagctacac
      781 agtatctaac atcaccctct gcaccctgtt taatgtaagc aaatacatag tactcttttc
      841 ctgaaaaatc aaaattttta tctagtagaa atattaaatc ttcatcttct gaataattag
      901 gttcgtaata aaatttcttc acacctaata gttttttcac tgcattagca gcatcttcta
      961 ctgtatattc tcttccagta ttagaatcgc ctatattttt tgttacattt aaacactgct
     1021 gtaatatact atcactcaaa tttcctatag cagttaattt agttgtactt ttcaatacac
     1081 ttttattact atcattatca actaacacaa tagcattatt tttagcaaca gtagaaccaa
     1141 caagagcata cactaaatca gtaccacttg ctatataaaa ttcttttact ccactataaa
     1201 atttatttat tatctttttg ttagtatcat atctatctgt cccacctaat ctagttgaat
     1261 tagtatcctt aactattttg tcattcattg atgaagtacc acctattaca taactttcta
     1321 caccagttgt attaaatgga acactttttc catcagttaa tactatagct gctttatctc
     1381 taactgctac aggtgcagca ctcattgcat caggctctcc tttaaatgca ttagtcaata
     1441 tgactttatt tactttgtta attgagttta tttcttttgc gacattatag cttgtcttaa
     1501 ttctatcact accttgaagt cttttaatct ctattccttt gccttttaga acagtttcag
     1561 tatatttatc aatagagttt tctcctccaa ttatatatac tttttttgcc ttttctaatc
     1621 ttttaagtgt agcatttggt atgttatttt tcttagtcaa aagaattgga gcgtttatag
     1681 ctcctgcaag tccacttgca cttaatccat cagccattgt actgtctgca ttaattaata
     1741 tagctgtagt atagttttgt ttgtctgcaa ttataccagc agtttcgtat ttatctgcac
     1801 ctttaattga ttcaatttta tcaagagcat ttacagatat agggcaacta gcaataaata
     1861 tagataaaga taaccctatt gttaataatt ttttaatttt cataatatct ccttctctta
     1921 aatattttca cctaaattat ataatacttt tcaggaaaat tttaccataa atttaaaagg
     1981 ttattattac ttaatataat attaatgaaa aatatcactc ttaaattaat gactttggta
     2041 gaatatggtg ataaaataaa attaatgaga gacaatataa gggggattat attatgataa
     2101 tcagtaaaaa aataacagca atattagtat catttttaat tatgatatca atggttggat
     2161 gtgctccaaa agaaagtgct acagaagtag ctaaaaagta ccttgatagt atagaaatca
     2221 aagactacga agtagcatat aatttattaa gtaaagacag taaggaaagt gtcagtctag
     2281 aaaaatttga agaatatcaa gatactttat ttaaacttca aaccctaaaa gattataaac
     2341 ttggtgaaga aaataaagta gagaaataca aatattctaa taaagagtac acaaatcttg
     2401 ttaaaataaa agaaactgat actataaagt atatcactga aaataaagaa gatactgcaa
     2461 attttgatag atattttata atcgaagata atgaatataa attattatgg cataaagact
     2521 ttaaaagagc tcttagctct aattatgtca aaatcgctag gtcagaaaca gaaaaagatg
     2581 aagataagga ttataataaa gctgctattg atatcaaaaa agctatagaa attgatgata
     2641 aaaatccact tgcatattat acacaatcat atatttatat agagttacaa cgatatgatg
     2701 aggctttaaa gtcaattgat aaatacttag aatatctaga taaaaataat gaagcttata
     2761 aaatagacgc tagtgatgct tacaatttaa gaggtgttat ttttatgagt aaatcagaat
     2821 ttaataaagc tagagaatct tacaataagg cgcttgaact taacccggat aatgaatatg
     2881 caaaaactaa tctaaatgat attaaaggat tataaggcat taatattata gaaaataagt
     2941 atgtcaaaag ttaaattaac ttttgacatg cttattttct atattttaaa ctctattgat
     3001 ttactatata atagtgcata aggagtgtga ttacttatta tgagtgttgc catatactta
     3061 agaaaatccc gtgctgatga agaggcagaa aaacaaggcg aatttgaaac tttaagtaga
     3121 cataaatcaa ccctactaaa actagcaaaa gaacaaaact tagatgtaat tgagataaaa
     3181 gaagaattag tttctggtga aagtataata catagaccca aaatgcttga acttctaaaa
     3241 gaagttgaag aaaataaata tgatgcagtc ttagtaatgg acttagacag acttggtcgt
     3301 ggagacatga aagaccaagg tatcatatta gaaacattca aagaaagtaa gaccaaaatc
     3361 atcacaccta gaaaaacata cgacctaaca gatgaatttg atgaggagta ttcagaattt
     3421 gaagcattta tggcaagaaa agaattaaaa cttatatcaa gaaggatgca aagaggtaga
     3481 ataaaatcag ttgaagaagg gaatttcata ggaacctctg ctccatttgg atatgatgct
     3541 gtaacaactg gtagaaaaga aagaatactt gtaccaaata aagatgctga tgtagttagg
     3601 actatctttg acttatatat taatgaagat atgggttgta gtaaaatatc aaaataccta
     3661 aataacttag gcattaaaac tgctacaggt gctaattggt acaattctgc aattacaaat
     3721 ataataaaaa ataaggcata ttgtggctac attcagtggc aaaaaaagga ctacaaaaaa
     3781 tctaaaaatc caaataaaat aaaaacagtt aaactaagac ctaaagatga atggattgag
     3841 gcaaaaggaa agcatgaacc tttaataagt gaaataacat ggaaaaaagc tcaaaatata
     3901 ctcaaaaaaa atggtcatgt cagctatggt aatcaaataa aaaatccact tgcaggaata
     3961 gtgatttgca aaaattgtgg tagaccatta gtatatagac catatgcaga ccacgactat
     4021 ataatttgtt atcatcctgg ttgcaataaa agttctcgat ttgaatttat tgaagctgcc
     4081 attttaaaat ctttggaaga cactgtaaaa aaatatcaat taaaagcatc tgatatagac
     4141 ttagataaaa ataataaaga tagtaatata gaatttcaaa aacgagtctt aaagggctta
     4201 gaaactgaat taaaagaatt aggcaagcaa aaaaataagt tatatgattt actagaacga
     4261 ggaatatatg atgaagatac atttattgaa aggtccaaca atataagttc aagaactgaa
     4321 gaaattaaag attcaattaa gacagtaaaa aatagattga gtactgttaa aaaagacaat
     4381 tctaaaataa tagaagatat taaaactgtg ttatcacttt accatgattc agattccctt
     4441 ggaaaaaata aactcctaaa atctgtaatt gataaagcag tttattataa gtctaaagaa
     4501 caaaaactag attcttttga attaatggtc catttgaaat tacatgaaga ccagtaatac
     4561 caataacata taaagagtag tgtgtaatga aataagtctt ttagatatac ttggtacaga
     4621 ggctaactat gttcttgatg aagttgaact aaaagttcaa gttggaaaac tttacgaaca
     4681 actaaacaaa attcttactc ctagagaaag agaaattgtc cagttaagat atgggttaac
     4741 tccttatggc tataagacac aaagagaaat tgcacaaaaa ctcgatatat caaggtcata
     4801 tgtatctaga atcgaaaaaa aagctcttaa aaaactagaa aaagagcttg ttcaagaaag
     4861 ttaatactat attttaaaag gatatatcgt acatcattag acaaaatttt ctatctaaaa
     4921 tcaaagttac gatatatcct taaattttat agtacataat ttattttata ctttcacata
     4981 tttccttaaa tataggtgtt gcagatttat ttcctttttg agttccttct actaacacag
     5041 ttattacata cttaggtctt tcttcaggat aaaatcctgt tatccatccg tggtctatag
     5101 cttttttatt taaactactt tgagctgtac cagttttaac tccacaacca ccttcaatat
     5161 cttttaggtc tttagctgta ccagtcctag atacagactt catatattct ttaacttgtg
     5221 tacacacata aggagaaata agttcttctt tttttgaaga cttataagtt tttattgtat
     5281 tcatattatt gtccacaaga cttttatata agtatagtgg tttgaatgtt ccattattag
     5341 ctattatttg agtcatttga tttatttgaa gtggtgtaaa ttctatattt tcttgaccta
     5401 tagctaaatt tcttattgat atgttttttg gagcttctct aattttttct tcatcaagac
     5461 caatatcaac cttttcaaat aaatgtaatt tttcagcaga tttgagtatc ttttcttttc
     5521 caagtttcat agctatatct aaaaatgcag gattgcatga attagaaaaa gcttgtctta
     5581 agttttggaa accatgacca tctcttttat tacatcgcag tatttcatta gtattcccaa
     5641 ctttagtctt acctgtacag ttgtaagtat agttctcatc tataacacca ttttcaagtg
     5701 cagaaaataa aactaccatc ttaaatacag accctggtgg atatgtcgct tttataactc
     5761 tattttcaaa ttctccattt tttcctttta aactttttga tatgtcattt tggtcaaaat
     5821 taggtctaga gcacattgca agaatttcac ctgtagatgc ttccgagatt attgctgctg
     5881 taggattttc ttctttattt agtatttgtt ctaatttttt ttgaatgtta taatcaatag
     5941 tagtttttag atgtctatct ttatccttat tatcaacagt ttttacactt ccctttagta
     6001 tgtttagaga tttttcattt cctgcatctc cagctttaaa agcacttata tacttttcat
     6061 ttgaatttct aagtacacta tccatactct tttcaattcc tgattgacct gatttatcat
     6121 cttcttttat atatcctatt gtatgtgata ataaaccatc ttttgcgtac ctcatagttt
     6181 tttcttctac cattattcca tttttctcaa gttctttttt catactctta tcgatatcga
     6241 tatttttagt ctgaatctgt attatagggc gtgtcaattg ctcttgtaca gctttatata
     6301 tatctaaatc attcatctta gtagctttct taataagctc caaaatttta tagttattat
     6361 ttaatttttc tttttcaaca attaatacct gagatttact tgtatctgta agcttcttgt
     6421 tgtttctatc atagattatt cctcttccac tatttaactc gactttctca acgctttgtg
     6481 attcaacact ttgtttgtat ttatcacctt ttaatacttg tatatcaact agcctataaa
     6541 ttaaaacact ataaattatt aaaattatag taaatataca ccagcttctc tttcccactt
     6601 ttttcaaaaa tggagtcttc ttttttgaca tgaaatcacc ccttagataa tttcattatt
     6661 gacaatttct aaggggctaa tactattttt atcaattttt tattattctt caaccttttc
     6721 ttctataact tttctaagca tataatcttt accaactttt actgatagct ttaacttcac
     6781 tatttgtcta ggatgaggag ctgattctat agcttctcct tcttcattat acatttcttc
     6841 aattttagta aacatagttt ctttatatgg tcccataact tctatttcat caccaacaca
     6901 cattttattt ctttgctcaa caacaataag accatcttca tcttctgttt ctctaactat
     6961 acctataaaa tcatagtttc taacatatga tgctgattga taattttggt cttctgttgt
     7021 tggtttattc aagtaaaaac ctgatgtaaa atgtctatga ctaccttttt ttagttcttc
     7081 catccacatt ggattaaact tccagttttc tgggtctctg taaaattcat ctattgccat
     7141 tctgtatgct cttacaacag tagctacata gtatgcagtc ttcattctac cttctatttt
     7201 taaacttgtt ataccagatt tgattaactc tggtatatac tctatcatac ataaatcttt
     7261 tgagttaaag aagaatgtac ccctttcatc ttcgtatatt ggaaaatact caccaggtct
     7321 tttttcttcc accaaatgat attgccatct acaagattga gcacaagaac ctttgtttgc
     7381 atctcttcca gtcatatagt tgcttattaa acatctgcca gaataagaaa tacacatagc
     7441 accatgcata aacgcttcta tatccatatc taaaggtgtt tttgctctta tctcatatat
     7501 ttcatcaaaa gataattctc ttgccactac aactcttttt attccatgct cgtaccagaa
     7561 gtttgcactt agataattgg ttgtatttgc ttgtgtactt aaatgtattt ccatattcgg
     7621 aattactttt ttaatgacac taagcactcc tgggtcagct attataactg catctattcc
     7681 tatctcttct agttccaaca aatagtcttc taattgtaga aaatcttcat tatgaggtat
     7741 tatatttact gttacgaata ctttcttgcc tctttcatgt gcaaaagcta ctccctctgc
     7801 catatcttcc ttactaaaat tcttcgctgc agacctcatt ccaaaaattt ctccacctat
     7861 ataaacggca tctgctccat atgttatagc tattttcaat ctctccaaat cacctgctgg
     7921 agctaaaagt tctactttat ttaattccat ctacatcacc tcttttactt tctttataac
     7981 ttagtgctac accatcacct attggtacca atgatgtgct taaatagtca caattacata
     8041 tataatctaa atactttctc attcttttaa ctatagtctt ttttcttctt acaacaaaat
     8101 catcatgagc aaccatacct ttatacaaaa tattgtcaga tactaaaagt cccccatctt
     8161 ttaatttatc tataactaaa tcaaaaaata gcttatattg acctttggct gcatctataa
     8221 atataaggtc aaattcacct tggacttgac taagtttttc ttctgcatct ccttctagta
     8281 tagttatgtt attgtcaaat ccagctaatt ttatgttttc ttttgctttt tctatcattt
     8341 tttcatttct ctcaacagtt attatatctg catcttttcc tataatagtt gcaaaaaaaa
     8401 tagaagaata accaattgca catcctactt ctaaaattct ctttggtttt tgaaccttta
     8461 acaagacttt taataaatct gaaacttctt tatgtattat aggaacacta ttttcctctg
     8521 cataaatttc taaatctttt aaaaatcctt ctttttcttt taatgtcact cttatataat
     8581 cttcaaccaa atcattaact atattactca taaaaacact ccctaaaata ataaatatcc
     8641 aagttttata ttaatttatt attttttaaa ttcatattgt tatatatatt tacactttct
     8701 ttattataaa tctaagaata acaattttca ataaaaaagc ccaacattat tcttaccctg
     8761 ttagactttt tataatcatg gtttattttt ttgtattttc tttatttgtt gcttttgtat
     8821 cttttgattg tttatccctt gcttctttat attctttaac gtattttaaa tggtcttgat
     8881 agtttgtact ataattattt ccaccatcaa tcttagctac aaaatataaa tagtctgttt
     8941 tctctggata taatgctgct tcaatagatt ttataccagg atttgatatt ggtgtaggag
     9001 gtaatccctt atttttataa ctattatatg gtgaatcaat ttttaaatca tcatatgtta
     9061 caattttctt tctctcttca aatatatatt gtattgtagc atctgattga agaggcatac
     9121 ctaccttaag tctattataa aacacacttg ctattatagg tctgtctttg tcaagaacag
     9181 cttctttctc aattattgat gccatttcca ttacttcttg aagagtcata tttaattctt
     9241 tttgtttctt tttaaacttg tccgtgtatt tactgtcaaa tacttttaac atttcgctta
     9301 aaatatcttc ttctgattgt ttctttgaat taaaataata agtttctgga tacaagaacc
     9361 cttctaaaga ggttattcca tcttccttca aatatgaaaa tttatcataa aattcttgag
     9421 gtttttttat taaattttca aaagtagctt tatctcctaa atttttactt actaacattg
     9481 ctataatttc tttagatgta gctccctctg gaatagtcac ttttatacca tcctgatata
     9541 ttttacctga aactaaaaga cttattatat cattgtttga atacgattga ttaagtaagt
     9601 atgttcctga ttttatagac ggagctttat tgctaacctt taccaaaagt ttaaataata
     9661 gttcattctt tattaattta ttttcatata atatgtcaga tatttttcca acagatgctc
     9721 cacttggaac gtcgataatt acatcttttt tgttattttt atcataagga cctatttgta
     9781 taaacacaaa tatacctgct aagataatca aaataacaat tattaatact gctattttca
     9841 atctattttc tttaaagttc ataatataat ctactcctta tgtatattta tccattttct
     9901 attataaaac tagatacttt aattttcaat cat
//
LOCUS       DAELTA010000135         9887 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.135, whole genome shotgun sequence.
ACCESSION   DAELTA010000135 DAELTA010000000
VERSION     DAELTA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.135"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            215..889
                     /locus_tag="KPG13_002838"
     CDS             215..889
                     /locus_tag="KPG13_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986538.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            913..1917
                     /locus_tag="KPG13_002839"
     CDS             913..1917
                     /locus_tag="KPG13_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986539.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            2029..3258
                     /locus_tag="KPG13_002840"
     CDS             2029..3258
                     /locus_tag="KPG13_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG0986540.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3266..3940
                     /locus_tag="KPG13_002841"
     CDS             3266..3940
                     /locus_tag="KPG13_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986541.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3943..5175
                     /locus_tag="KPG13_002842"
     CDS             3943..5175
                     /locus_tag="KPG13_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986542.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5461..6726)
                     /locus_tag="KPG13_002843"
     CDS             complement(5461..6726)
                     /locus_tag="KPG13_002843"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG0986543.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6779..7927)
                     /locus_tag="KPG13_002844"
     CDS             complement(6779..7927)
                     /locus_tag="KPG13_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG0986544.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(8018..8563)
                     /locus_tag="KPG13_002845"
     CDS             complement(8018..8563)
                     /locus_tag="KPG13_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0986545.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8786..9730)
                     /locus_tag="KPG13_002846"
     CDS             complement(8786..9730)
                     /locus_tag="KPG13_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG0986546.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3528 a         1513 c         1316 g         3530 t
ORIGIN      
        1 ttttaaacat ttaattatat taatttaagg ttacattttc gtaagtatta attatatttc
       61 ttcaaattta ttgcaattat gtttaaaata taatttatca agtaaaatat agttatatta
      121 atataattat tttaggtaat agggtagaaa tattttaatt tatcaagtag aatatagtta
      181 tgttaatata actattttga attaggaggt aagaatgttt aaaataatgg tagttgaaga
      241 tgacgtctca ttgaaaaata tcatagcaaa gtgtcttact aaatggggac atgatgttca
      301 tcaaatagaa aatcttgaaa atataataga agagttcaaa aactataacc cagaattagt
      361 tttattggac ataaaccttc cattttatga tggttttcat tggtgtaatg aaataagaaa
      421 aatttcaaaa gttcctataa tttttatttc ttcaagaaat tctaatatgg atgtaataat
      481 gggtgtaaat ttaggtgcag atgattatat acaaaaacct ttttctgtag atgtcttagt
      541 agctaaagtt aatgcccttt taagaagaac ttataatttt gttgataata actcaaatca
      601 gattattcac aatggtgtta ctttagattt atcaactgca acaataaact atgaagataa
      661 tactattgag ctaacaaaaa atgaaatcaa aattcttcat gagcttatga aatataaggg
      721 gcagatagta agtaggaata aactcatgaa aaaattatgg gataatgatt ggtttgtaga
      781 tgataataca ttaactgtca atgtaaatag aatacgctca aagttaaatg aaattggttt
      841 agaagatttt atagaaacaa aaagaggatt gggttatata atcagttaaa acaattctaa
      901 gtaggtgaat ttatgagctt taaagatttt ctcaaggata aactaggttt tttagtatac
      961 aattttactt tcttaatttt tacagtatca gttattatat tttctcctgt agatgttttt
     1021 ctgactgata ctattttata tatattagtt gtaaatgttg tgtttttatt tttatatcta
     1081 tttattagct acatcaagaa gaacaaattt ttagataaga taaaaaatga tacattcaaa
     1141 attaatatga atgatataga attagctaga tgtaaaaatg aagaaaaaat atacttgaac
     1201 attattaaaa atcatcaata tcaatgtaat aacataataa ataataatat cgagaaattt
     1261 gaagaaaaca aagagatttt aaatatgtgg gtacatgatg taaagatacc tatagctata
     1321 ataaaactca ttcttgaaca aaatgaaaat ttaattgatg aaaaaatttc agatgacata
     1381 gatagtgaaa tatttagaat tgaaaactcc gttgaaaaaa ttttatattt atcaagactc
     1441 gaagattttc ataaggattt tttaattcaa gaggtaaata ttgaaaaaat cattagaaat
     1501 attataaaaa aatactcaaa atattttatt tcaaaaaaaa ttattttaga cttaaataat
     1561 ttgaatttta aggttttatc tgatgaaaaa tggctatact tcatattcga gcaattattg
     1621 agtaattccc taaaatactc ctcattagga gataatatat ctatctattg caaaagaacc
     1681 aaaaattatt tacaattaag agtggaagat actggttgtg gtataaaaaa ggaagattta
     1741 aatcgaatat tcaataaggg atttacaggt aataatggaa gaaacaattc taaatctaca
     1801 ggtcttgggc tttatttagt aaaagaactt tgtgataagc ttggtcataa aattgatgta
     1861 gattcagagt acaaccaata tacaaaattt actattacat tcaaatgtga tttatagtat
     1921 cttattattg atagtttatt ttcattaact tatattcaac ttcttatagt aattctatat
     1981 gtacatccca gtatataatt aagtagtttt tataaaaagg aggaagacat gtcttttcta
     2041 aaaaaattta aaaataaaag atttagaaat aacagtttta aaagtaacaa ctttagaaat
     2101 agcagttttg gaagtagtgg cttcaagtct aaaaataaag gttttaaaaa caatcccttt
     2161 aaaaataaga gattcaaaat atatgcagga atagcagtaa tcatactttt aattctagga
     2221 gttcttgctt atgttgattc gaaaaacact gaattacaag ctaatgaaaa ttttatcgaa
     2281 acatatacta ttcctgaaaa tgaaaaaata tttataaatg ggatggttgt tcctaagcaa
     2341 actaaggact tcaatatatc tggagattat gagttaagtg atgttaatgt tacaaatggt
     2401 caaaaagtaa atcaaggaga ccttcttttt actgctaaaa atccaactat aatagcagaa
     2461 atagatagtt taaaatcaca acttagtcaa tacaaaaaac aaaaaatatc actttctgat
     2521 attgctgaaa ataaagatgc tattgcttct ataaatgcac agataactgc tttaagctcc
     2581 cagatagctt ctctggataa aaaagcttat gacagagtta ctgctccttt tgatggcaca
     2641 gtatacttga atgaccaaac tggaaatcca gaccaaccag tttcatttat gactattcaa
     2701 ggtctagagt tttatatgaa aggacaagca agtgaacaag atttacctaa aatgaagatt
     2761 gaccaaatgg tcaatatact agttttttca actgaccaaa agcttactgg tagaataagt
     2821 tttatatctg ataaaccatc tactccaaat actgaaatgg gagctcagca gaatacacta
     2881 tcttattatg atataaacat agcatttgat aatcaagaag gattagtaaa tggattccat
     2941 cttcaagcat cacttgaagt agctaattca agctttaaaa taccagcatc ttgtgtactt
     3001 aaagacaaca aacactcata tgtatttaag gatttagatg gtatattgaa aaagcaaatt
     3061 gtagatgtag caagtcaaaa tgatgatttt gctgtagtta gaggcggtct tgaacaaggc
     3121 gatataataa taaaacaccc tactaaagaa atgaaagaag gagacccagt tcaaggcgat
     3181 ggcgtaactg ctggttctaa taatggagat actactccaa acaaaaaagt agaagaagtt
     3241 actatggatg tgaattaatt tacatatgtt aattaaatta gaaaatattc aaaaatatta
     3301 caaagtaggg aaagatgaat tacatgtttt aaaatcctta aatttagaaa ttgaatctgg
     3361 agaatttgtc atgataatgg gtaagtctgg tagtggtaaa actactttac ttaatatctt
     3421 aggttttcta gatgtatttg atgaaggaag atatatattt gatggaacag atgtaactaa
     3481 tttgagtgaa aatgaacgtt ctgtttttag aaatattaat attggatttg ttttccaaca
     3541 atttaattta atagaaactc taaatgtata tcaaaatgta gagttgcctc taatttacaa
     3601 taaagcacta aaaaaatcta atagagaaga aattgttaaa gataaattaa gttctgttgg
     3661 gcttttggac aagcttaagc agaaaccact tcaattatct ggtggtcaac aacagcgtgt
     3721 agccattgca cgttgcctag caaatgaccc tcagataatc tttgctgacg aaccgactgg
     3781 ggcattagat agtgaaacta gcagagaaat tatggagctt ttaacaaggc tcaacaaaca
     3841 aggtaagact ataataatgg ttacacatga tcaagattta actaagtatg caactaaggt
     3901 tatacgactt aaagatggtg tgtttacttc ggaggtgtaa tcatgagttt attaaaaaat
     3961 tctttggcca atttaaaagg tcacaagctt cgtgtatttg tagctttact ctggattata
     4021 ataggaataa cttcagtaat acttgttagt tctataggta atggatttca aaaagaaatt
     4081 aagaaatctg taaacaatgt aaaccctaat aaaactacta tttcgtttga atcagccgat
     4141 aacacaggtc ttactgatga tatgagtatt tttttaaaac catttaatgc aaaagattta
     4201 gaagaattat catttgttga aggagtagaa agaatagctc cttcaagaga tgggtttaac
     4261 cttgattcag tttactcttc acaagcatct tttgataaaa aaactacgta tgttgatgtt
     4321 ggaccagtaa aaaaagattc taaaataaat ttaatttgtg gtagagattt ttctttagat
     4381 gatgaaaaaa gaaaagtaat tttacttaca ttacaaagta caagtgaaat ttttgaaaat
     4441 ccagaagatg ccttaggtca tggtatcaac ataaatggaa ctatatttga aataataggg
     4501 gtattagatg attctcagca aaaccaagct ggaggattct ttggaggtta ccaagatatg
     4561 caatttacta cttccctagt acctaaaaag gcttttgaca ctttaatgag tcaaaattct
     4621 tattcaaatg aaatttacca gttagactta gtttcttcta aaggctataa tgtaaatgaa
     4681 gttgcaaaca atgttatagc taaattatat gagatgcacc ctggtatcaa tggttcttat
     4741 actacaccag acccaactga acagacagct tatcttgaaa gcattaattc caatgtaaac
     4801 aaatatgtat ctataataac tgtagttgca atgtttgttg gtggtattgg tgtaatgaat
     4861 ataatgtatg tatctgttat ggaaagacaa agagaaattg gtataagacg tgctattgga
     4921 gcaaaaccta ggtctatatt atttcaattt ttagttgaag ctgtttttat aacagtatgt
     4981 ggtggaattt taggaactat agttggattt actgctacta actatgtttc taaatatata
     5041 ggttttgaag ctatacctag cttaaatagc ttattttatg caattgtagc tactatattg
     5101 actggagttg tatttggtct gattccagcc tttaaagcct ctaaactaga ccctataaag
     5161 gcaatttata agtagaaatt gtataaagat tttcgatttt tagattagtt tatataaaac
     5221 atacttaaat atataacgca ctattatata tgatagatta ggaacttaat ctttccaaat
     5281 ataatagtgc gttctttcat aaaaaggatt ctcttcctta ctatatctaa attaccaaga
     5341 tgttatgttc taattgttca aactaatact tccaattttg attaatcaat ctcattctcc
     5401 taatatacaa tcttttactt tcaatcattg tacactccgc ctaaaaaaaa taatcttaaa
     5461 ttaggcgtta tatacaatct ccccattaca tattgtataa ttaacctttc catataaaga
     5521 tttaccttta aatggtgaat tatcagcttt agaaacaaag ctttcaactg tccattcttc
     5581 ttctggattg aatataacaa tgtctgctac agccccttct tctataaatc caatatttaa
     5641 attgtacaac tttgcaggat ttatagacat tttttctatt aactgcatca tactaagatg
     5701 atttttatgc accaaatttg ttattccaag agctaaagaa gtttcaagcc caataattcc
     5761 acttggagct tttataaact ctctatcttt ttcttcttta ctatgaggtg catggtccgt
     5821 tgctattatt tcaatagtac catcatttaa tccctctata atcttatctc tgtcccattt
     5881 acttcttaga ggtggattca tttttgcatt tgttccaaac tccaatacat cttcctcagt
     5941 caatgtaaag tgatgtggtg aagcttctgc aactacattt gcacctaagt attttgcaaa
     6001 tctaaccata tcaacagata cacctgaact tatatgttgt atatcaactt tagcaccagt
     6061 ctttagtgcc aacatacaat ctctggctac cataacatct tctgctacac ttgatgcacc
     6121 ttttaatcca agttctgaag ctacctttcc tgcatttaca cctggatttc caactaaaga
     6181 agggtcttct tcatgaaaac taagtggtac atccaactct tttgccttaa tcattgcctc
     6241 cataattaga ttgctatcca ttaaaggtaa tccatcatca gtaaaccctg gtacaccagc
     6301 ttttttaaga gcttccatat caactaactc tttaccttca aaacctttag ttatagctgc
     6361 tgcttgtaaa acatttatac atgcattttt tgacttttct ttaacataat taaaagtatc
     6421 ttcattatct acaataggat tagtatttgc catacatata acagttgtaa atccacctct
     6481 agcagcagat ttagctccac tttctatatc ttctttatat gtaaacccag ggtctctaaa
     6541 gtgtacatgt acatccacta aacctggagc aactacatta ccactagcat ctattatttt
     6601 ttcatattca tcactttcat caaacttgcc tattttataa attttatttt cttttataat
     6661 taagtcaata ttctcatctc tttgactttt agggtctatt actctaccat ttttaattaa
     6721 aatcatatta tttctccttt atatttttaa tttatataga ctcatataat attatacatt
     6781 attttacata cttaaataca aaattacgtt tgacatcaat agccttttta ggacaaagtt
     6841 catggcaaca gtaacatctt atacatttac ttaagtctat aatagcaccc ttagtcccta
     6901 tctctatagt ggatgcagga caagcttcct tgcattttcc acattttatg caatcttgaa
     6961 atcttaccac aggctttggt gttataagct ttgaaatagg ttcatttaaa aattttggta
     7021 gctttacaga aaacaatctt aaatccttac ttacatttgg aatcttgtaa ttttttataa
     7081 ttaaatcttt tatatcttca cctaaaattt caatatctga aaaatcttct tgtatatatc
     7141 ctctcttaat agcacctcta attgtagcaa ctctatttgg aagtaaactt ataatcttac
     7201 atgcaacaac atctatagca tatggatttt gtgatgctaa taaaacgcct atttctcgag
     7261 tagtaccagc tgaaggtcca tcaccttcca tacctactat accatccata attgtcaatg
     7321 ttggttttat ataatcacaa atatctaaaa gtacattttc gcaaaacaac tcttctgtag
     7381 gaaatctata atgtatctct gcttttttca atccagctat gcatccatat aagttttttg
     7441 taccaccagt aaaagttgcc attatatgag tctttaattt acaaagattt attatataat
     7501 ctgaatctac tattggttta ataatatcca tatattttag aacatgtgct ttttcactat
     7561 gtatcttaac atctgaaaca tcataattta acttaatgcc cagttcattt gctacttctt
     7621 caattccaga tgccttataa atgctcttaa gagaactttg attatatgga ccaccagggc
     7681 tatctgctat tgtaatatca caatttaact ctaaaagttt ttcacataca acttttacaa
     7741 ccataggatg tgtagttgta gcttcttcag gctttttttt cattaataaa tttggtttta
     7801 taagtacttt gcttcctttt ttaacaaact tatctatacc accaatatcc tctaaatttt
     7861 tttcaataga taactttact tcttcataat tgtaagtttt acaactccta acagataccg
     7921 acttcatatt atctccatcc ttctatcaca acagtttatt tgtataattt aaaaaaggag
     7981 aaaccaaagt ttctcctttt aatctatgtt ttaattatta ttttccaaaa tgtctgttta
     8041 ataatcctaa gaatgcttta ccatgtctag cttcgtcttt acacatttca tgtactgtat
     8101 catgtatagc atctaatcct aattctttag ctctcttagc taattttaat tttccatcag
     8161 ttgcaccata ctcagcatca actctaactc ttaagttttc ttttgtatct gcaactacaa
     8221 cttctccaag aagttctgca aatttagcag catgttcagc ctcttcaaaa gctattctct
     8281 tatacgcttc agctacttct ggataacctt ctctatcagc ttgtctactc attgctaaat
     8341 acattcctac ttctgtacac tcaccagtaa agttggctct taatccttcg attatttctg
     8401 catctacacc ttgagctact cctattctat gttcatcagc ccaaaccatt tcgcctttca
     8461 tttcttcaaa tttatcagct ccaactttac atactggaca ttgtgctggt gcagcatctc
     8521 cttcatgtat atatccacat actgtacaaa caaatttttt cataattaat ttcctcccaa
     8581 ttttatataa ttaaattttt atttttaagt ttaataactt gctacaagaa tatatatacc
     8641 ccagacttta tagcttaaac actaaaaata aaatttattt cctaattaat tttatcactt
     8701 tgaaacacca gtatcaacca agttttgatg tcaaatttct acataataca taatttattt
     8761 gattaattag gaaatagttg tcatattaaa tactttctaa tgaagactcc tctgaatctt
     8821 taagtttcac ttttttgctc tttaaaaaag ataatttagt ttctgcagtt atgattgcaa
     8881 caaatatcaa gatactacca ataaccaatt ttgctgtaat aagctccttt aaaattatta
     8941 tagaaaatat tgtaccaaat acagcctctg tggataatat tatagctgtt ctagttccat
     9001 caactctttt ttgacatata gtctgaaata aaaatccaat tgtagtacta aatacagcta
     9061 gatacatagt tcccatgtaa ccagataatt cagcttccat ctttaattga cctgcaaatg
     9121 tttgaactac aacagacatt aaaaacgcta ctgtaaattg aatagctgtc aatgccattg
     9181 ggttgttatc ttttgcaaac tcacttgtaa agaatatatg aaatgcaaat ccgaatgaac
     9241 atataagtgt tagaaaatct ccaaaattaa tagagaaatc agcttctaaa gaaagtatcc
     9301 caattcctat aagagctacc aaactactta ttattcctat tttatcaagt tttcttctat
     9361 aaagtataaa tcctatgaat ggaactatta caacattagc tgctgttata aacgcatttt
     9421 tagatggtgt agtgtacata agtccaatag tttgtactgt gaatgctaga aataaaaata
     9481 ttcctaaaat ccctcctgct tttaataatt ttttacccat attagcacga atctgtttaa
     9541 aaaacagtaa attcataatt atagctgcca agaaaaatct taaagttatg atttgtagag
     9601 gtgtaagccc accgtctaat gcaaattggg ttgctacaaa gccacttccc catataattg
     9661 ctataaatat tagtcctatt tctccaaagt attttttcaa taaagccccc tcctttttat
     9721 ttttttgcaa aattataaac cccctaaaca gcaagagagt ttttctataa gcattactat
     9781 aaattaatat agtaaatttt ttcagtattt taaatctaaa tttacataca ttttgagctc
     9841 aaatttatat ccactattta acagtaatat attaatcatt ttttgta
//
LOCUS       DAELTA010000136         9868 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.136, whole genome shotgun sequence.
ACCESSION   DAELTA010000136 DAELTA010000000
VERSION     DAELTA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.136"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..323
                     /locus_tag="KPG13_002847"
     CDS             <1..323
                     /locus_tag="KPG13_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0986547.1"
                     /translation="NKIIKILIGELEENIKESEEFKDLKDSFIISIENMIETGKINDA
                     KLLVDDYSKTFVDDIKILNIKGILYMFENKLDKADFMLKKALSLDVENEDTIYNIEYL
                     KSLR"
     gene            347..2224
                     /locus_tag="KPG13_002848"
     CDS             347..2224
                     /locus_tag="KPG13_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG0986548.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            2217..4331
                     /locus_tag="KPG13_002849"
     CDS             2217..4331
                     /locus_tag="KPG13_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0986549.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            4340..5335
                     /locus_tag="KPG13_002850"
     CDS             4340..5335
                     /locus_tag="KPG13_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG0986550.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            5427..6659
                     /locus_tag="KPG13_002851"
     CDS             5427..6659
                     /locus_tag="KPG13_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG0986551.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            6679..7623
                     /locus_tag="KPG13_002852"
     CDS             6679..7623
                     /locus_tag="KPG13_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG0986552.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            7717..8124
                     /locus_tag="KPG13_002853"
     CDS             7717..8124
                     /locus_tag="KPG13_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG0986553.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            8211..9302
                     /locus_tag="KPG13_002854"
     CDS             8211..9302
                     /locus_tag="KPG13_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG0986554.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      9611..9701
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         4022 a          864 c         1694 g         3288 t
ORIGIN      
        1 ataataaaat aataaaaata cttataggtg aattagaaga aaatataaaa gaaagtgaag
       61 aatttaagga tttaaaagat agttttatta ttagtataga aaatatgatt gaaactggca
      121 aaataaatga tgcgaaatta ttggtagatg actattcaaa aacatttgtg gatgatataa
      181 agattttaaa tataaaggga attttataca tgtttgaaaa taagcttgat aaagctgatt
      241 ttatgttaaa aaaggcatta tcattagatg tagaaaatga agatactata tataatatag
      301 aatacttaaa aagtttaaga taagtcaaaa ataggagaat tgaagaatga atataccaat
      361 agtggttaat gaaaaatcta aagacataat aaaagattgg caatatacat gtacaaatga
      421 agatataaaa tttacaagca taataatatt gacatataat caaatagaat atactaaatt
      481 atgtattgaa agtattagga aatttacccc aaagaataaa tatgaaataa taataattga
      541 taataactct tctgatggaa cagttgagtg gttaaaggga caatctgata taaaatctgt
      601 gtataataac aaaaacttag gatttccaaa aggatgcaat caaggtattg agttagctac
      661 tggtgaaaac atattgttgt taaataatga tgtaatagtt actccaaatt ggctctataa
      721 tttgaataaa gctttatggt ctcaagataa tataggtgct gtaggaccta ttacgaatgc
      781 atgttcatat tatcagcaaa ttgatgttga ttataagtca attgaggaca tgtttatatt
      841 tgcacacaaa ataaataaat ctaatattaa cctatggaaa tatagattga agttagttgg
      901 attttgcatg ttaataaaaa aaactgttct ggataatgta ggggttttag atgaattatt
      961 cacacctgga aattttgaag atgatgattt atcttttagg atgatagagt caggatataa
     1021 gttattatta tgccaggata cttttataca ccattttgga agtgtgtctt ttaaagaaaa
     1081 tcgtaataaa tatacagata taatgtatat taatagaaga aaatttgaaa ctaagtgggg
     1141 atttaatcca gaatatagtg gaatgataag aacagatttg atttctaaaa tagatgatag
     1201 tttggaggca aatattaatg ttttagaaat aggatgcggt ataggggcta ctcttttaga
     1261 aataaaaaat atatataaaa atgcatcaat atatggaata gaaattagtg agtcagcagg
     1321 taaacttgca aagtatagtg cagatgtact tatagcaaat atagaaaaag taaaattgga
     1381 ttataaaaaa gatttttttg attatataat attgggagat gtacttgaac atttgattga
     1441 cccatggaag atagtcaatg aattgaaaca atatctaaag aaggatggtt atctggtatc
     1501 tagtatacca aatattatgc atgtttctgt tttaagaaat cttgtgttag gtaattttac
     1561 atatgaagat tcaggaattt tggacaagac tcatttaagg ttttttacat taaatgagat
     1621 aacaaaaatg tttaatgata gtaattacat aattaatagt attacagcta ctagtatgcc
     1681 actttcggaa attgataata gcttcattga tacaatatgt aaggaaagct gtaaagaaat
     1741 gagacaacag tacgaatctt atcaatatat agtaaaagcc tctaaaatcc tagatactaa
     1801 aagatattct agtattagta tgattaattt aaaatacgct ttaatgagaa ttgataatga
     1861 attagatgta gatagtaatt taagttatat atttgataat tataatataa ctgaaccatg
     1921 ttttgttgat gatattgaat atctaattga tacatttgtg ataaataaag caactctatt
     1981 aaatagatta ggagttgagg catttaacag aggtctagga gattttagta taaacatgtt
     2041 tatgacagta ttagagatgg atagagatga tattgactca atatataata taacaagtgt
     2101 attatgtgaa ttagaagaat ttgatactgc ttataattta attaaaaata gtagcgaaaa
     2161 agtacagagt gaaagtgata tacaagagat actttgtttt attgagggga agaaatatga
     2221 ataatgataa gatatgtttt ataacctgtg taaatgatga aaggctttat gaagaatgtc
     2281 ttgtatatat aaatagccta gacattccta aaaactttag tatagaaaca gtagctttaa
     2341 gaggagccga ttctattgcc tctgcttata ataaagctat gagagaaaca gattctaagt
     2401 ataaggtata cttacatcaa gatactttta taattaataa aaatttcata catgatattt
     2461 tagagatatt taaaaaaaat acagatgtcg gaatgattgg tgtagctgga gtaaaagata
     2521 tgcctgtgaa tggaatttgg tgggattctg ataaaaaagc agggaaagta tatgatagtc
     2581 atacaggtaa aatggatatt tatgaattta gtaactttga agaatgtttt aatgaagtta
     2641 tggctataga tggattaatt atgattactc aatatgatgt attttggaga gaggataagt
     2701 ttgatgggtg gcatttttat gatttgtcac agagtatgga gtttagacaa aataaataca
     2761 aagttattgt accaaaacaa aataagcctt ggtgtatgca tgattctgga gtagtaaata
     2821 taaataatgg atatgatgaa tataggataa aatttgtaaa agagtacata aatcttgatt
     2881 tatatagaga aaatttttat gaatttgata agaagaaagc aagtaccata cttaagagta
     2941 ataagcatgt aatggattgt atgaaagagg agcttacaga acaattaaga ttaaagaatt
     3001 acaaggatgt tttaggtatt gtttctaaaa ttgctcattt ttctgttttt aatcatactg
     3061 gtttgtattc ttgtgaagaa tgtgagagag cattgctaga atgtgcaaat aatttaccag
     3121 aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa aagaagaaaa gtattacatg
     3181 ttttaagtga aggatattca acaggaggtc atacaagact agttaaaaat tggattgaaa
     3241 ttgacaaaga ctctgttcat tcagttgtaa ctacatggca atcagataca ctgccagact
     3301 ggttattaaa agcaataaat gatagtggtg gcgaagttat atctttggat ttaatttcag
     3361 atgattattt aaaaagagct gtagctttga ggaaaatagc ttatgactgg gctgatatag
     3421 ttattttata tgttcatatg tgggatacaa tacctgttgt tgcttttgga gttgatggga
     3481 aaatacctat tttacatgtt aatcactatg atagtacttt ttggttaggg acaagtattg
     3541 cagatattgt cattgatttt agcgaagcta gtcaattaat tacattagat aaaagaggca
     3601 tagataaatc tgaaatatta ccaatgccat taaaagaaaa agaagtattt tcaaaatctg
     3661 aagttagaaa acgatataat ttagaggatg atgtgacaat aatattgact attgctagtg
     3721 aaagaaaata caatagtgta aatgatatta attactttaa aattgctaaa acattgattg
     3781 aaaatacaga taatacaatt attattgttg taggagttga tacagagaat ctctattgga
     3841 aaactttaag taacgttaca gataatagag ttatatgttt tggaacaaca gcaaatatag
     3901 acgattttta caagatttcg gatatatata tggagtcttt catggtagga agtcatactt
     3961 caaaactaga tgcaatttta tatgatttaa ttcctgttaa gtttaaaaat gaagtttcac
     4021 caatttttac aaatatgtgg aattttatag ataagtttga ttataagaat ataaaagagg
     4081 ttatagatat gataaaaagg tacaatacta gtgaatttga cagggatagg ataaaagaaa
     4141 aacaatcgga aataaaacat tttgttatag ataaacactg tattgaaata aaaaactata
     4201 tagacaaaat atataatgaa gctaatcatc ataaagttaa gtatgattta tataaaaatg
     4261 atgaaatcga ggactatcag ctttttgtag cattatatgg ctatatggta aaaaataaaa
     4321 acatgtcata ggtggtaaaa tgaacaatat tcatatcaat aaatttgaaa aaagctgtga
     4381 aagaaactgg gattattttg tagaaaataa atctataaat ggtacatttc tgcaaactag
     4441 aaatttcctt aattatcatg aggagggaaa attcactgac cactcattaa taatatgtaa
     4501 aggtgaagaa atactagcag tcgtacctgc atgtgaaact tatgaagatg gtgaaagaat
     4561 atttttttct catttaggga gtacttttgg tggtattata ataagaagtg gttttaatga
     4621 tattaggcat gtaaatgcca ttacaaataa gttagaaata tatttacggg aacaaaattt
     4681 cgataaagta tatttaaaaa atacatcaag aatattttgt aaagaggata cttcattact
     4741 agaatatttt ttatttaaga ataattacat gttttatgat gaattaagta tgtttttaga
     4801 attaagtaag tataaagaag atatattagc caattttaaa tacagtaaaa gaagagattt
     4861 taaatattct ttgaagaata atcttacctt taaaagactg tatagtgatg aagatatagt
     4921 ttgttttttt aatttacttt ctaattgttt agatagtaaa ggagctaaag taatacattc
     4981 agtagaagaa cttttggagt ttaaaaataa tagattaaaa gatattgtag aattttatgc
     5041 agtatatatt gatgacatta tgatttgtgg gagcatggta tttaactttc aagaaagagt
     5101 ttatcatact caatatctag catcaaattc gacatataat aaatattatc caatgatatt
     5161 tttatactat aatctaataa aagatgcttt agagagaaat tttaagtatc tttcttttgg
     5221 aattagcact ctaaatagag gcaaatactt agacgagggt ttagctacat ttaaagaagg
     5281 ttttggttca agtgcaattt gtaacagaac atactataaa aatataaata agtgagaatc
     5341 atgttttata gagtttttag aatatagtaa atatctagta ctctaacatt acataaaaat
     5401 aattagttta taaagaaggt ttgtatatga aaagattaat gattttaggc tcaattaagt
     5461 attttgagaa tattgttata tcagctaaaa aagagggaat atatacgatt gtttgtgata
     5521 atagaattga tactcctgcg aaaaagattt gtgatgaagc aatagatgtt gatgtatttg
     5581 attttgataa aataaaaagt atagctatag ataaaaagat agatggaatt ttaactggat
     5641 ttacagattc tttaatgaaa ccatatgttt atattgcaaa tgaactaaat ttaccatgtg
     5701 tgatttcttg caatcaactt gaatctgtaa ctaacaaggc tattatgaaa aaatatttta
     5761 aaaataatgc aatacctaca actgattact gtattattga aagtctaaaa gatttagaaa
     5821 aaatacaagg attaaatttt ccacttgtaa taaaacctgt tgatggttat ggttcaagag
     5881 gtgtatattt tgtatcaaat atagaagaat taatagaaaa atttgaatat tctaaggaag
     5941 cctcacaaga tggacaagta ataattgagg aattttatga aagtgaggaa atacaaggac
     6001 tagcttgggt tcataatgga gagtcacatg tattttatat tggagataga gagcttgtaa
     6061 acatacatac aggaagagct ggaaaaccag atagattact ttatccatca aagtattgtt
     6121 ttcaatatga ggaggaaata aaatctatat atcaaaatat aacacaggct tttgatataa
     6181 aaaatggacc tctttatgta caaatgttag tgggagcata tggaataaag gtatctgaag
     6241 taatgccaag attacctgga ggttgtgact atatggctat ttctcaaata acagatttag
     6301 acataggaag tttatttgca aactttagtg tgaataatga agtaaatttt gatgaaataa
     6361 agaaatataa tatgcatcta tcaaaatgtg tatatgcatt accaatatac ataagacccg
     6421 ggacaataaa aaagataaat aatatagata aaatagaaaa cttagattat gtcactcaat
     6481 ttctattact agtaaaagaa ggagatacaa tagaattata tgaagatgag agacaggatt
     6541 gtggaagagt ttatggaata gcaaataata tatttgatgc aaatataaaa aagaaatgta
     6601 ttcatgaaat gatagaaata cttgatgaaa ataatgaaaa tatgatagaa aatttttaaa
     6661 taagggagta tgatagatat gaagctaata actcatgaac aaattatgtc attaaacatt
     6721 tctcagaaac aaggatatga gtggataaaa gaatctttct taacaaaaaa agattgtcaa
     6781 ttaccaccaa aaataagcat ggtagaagaa ggacatgtat tttacaatgt aatgccatcc
     6841 ctaaatcata atcatatgat tggtggaata aaaatggtta caagattgcc agggagaaat
     6901 ccaagtttaa actcagagat atttctatac aatatggaag atgctaaatt aaaggctatt
     6961 ttggatggta attatattac agcttttagg actgctgctg ttgctgcctt ggctatcgat
     7021 acatttgcta aaaaagattt taatactatt gctattatgg gtttaggtgt aactgcttct
     7081 gctataatga atatgctttc atatactctt aaagataaaa atataaatat aaagttactt
     7141 aaatataaag accaagcaga gttgtttgta aaaaagtatg aaaaaaatga acaatttagt
     7201 tttgaaatat gtgatacaca tgaagaactt attgtagatt cagatgtagt tatttctact
     7261 gtgacatata cagatgaata tttatcagaa tttaaatggt ttaaaaaagg tgctttatta
     7321 ttacctgtac atgttatggg atttggaaat gttgattatc tatttgataa agtatattta
     7381 gatgattata atcatttgaa acatctagaa aatatagata gatataaata ttcttgtgaa
     7441 ttttatgatg ttttaagtaa acaagctaaa ggaagagaaa atgatgatga gagaataata
     7501 tcttacaata taggtttgtc aatacatgat atattatttg caaataaaat ttacgaattg
     7561 tgtgaaacac taagctgtga agaaatagaa ttaaaagaac caaaagaaaa atattggata
     7621 tagtttattt cattcatatt tacaatagtt tattgtaaat atgaatatga agtaatatat
     7681 tgttagaaaa ttagcatgaa taggatagat aaatttatgg ataaatataa tttaattagg
     7741 ttcgtatcta ttggaaacga atatatagga aatttagttg cattagaatc aaataaagta
     7801 ataccttttg atataaagag agtttattac atatatgatg taccaaacaa tgtaaagaga
     7861 ggatttcatg cacacaaaaa cttggaacaa gtactagttt gcctaaatgg aagtgtgaag
     7921 ataaaatgtt atgatggtgt aaaagagact atatttaatt tacataaaaa tgatgttgga
     7981 ttatatatag gaacgggagt gtggcatgag atgtttgatt ttgatgaaaa aactgtttta
     8041 atttctatag catctgaaca ttataatgaa gaagattata ttagagacta taaagaattt
     8101 ttaaagtatt taaataatgt gtagttatta tacaattagt gatgtatttt cttaattaaa
     8161 taaatttaag atagccacat agagaataga tagattagga tgtgaattta ttgttagtta
     8221 aatttaataa tttattaaaa cagttccaaa ctcataaaga agaatatgaa cagaagatta
     8281 aggatgtaat ggaaagtgga tattatatcc ttggaaatga agtaaagcat tttgaagaag
     8341 aatttgctaa ctatgtaggt agcaaatatt gtgttggttt agccagtggt ttagatgcac
     8401 tgataattgc ttttagagca ttaggaataa gcaaaggtga tgaagtaatc gtgcaatcaa
     8461 atacatatat tgctagtgtt atgggaatca gtataaatgg agcaacacca gtatttgtag
     8521 aaccaaatga atattataat atagattcat ctaagataga agaaaagata aatagcaaaa
     8581 caaaagctat attagtagtg catttatatg gtcaagcaag taatatgaaa gatatacaag
     8641 aattatgtga taagtataac ttgtatttaa ttgaagattg tgctcaatct catggagcta
     8701 aatatgatgg aaaaatgaca ggaacatttg gtgatatagg ttgttttagt ttttatccaa
     8761 gtaaaaattt gggtgcattt ggagatgcag gagctatagt aactagtaat gaggaaatag
     8821 ctgataaagt gaaaattctt agaaactatg gctcagaaaa gcgatattat aataaatttg
     8881 tcggatataa ttcaagattg gacgaaattc aggcaggttt attaagagta aaacttaaat
     8941 atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga tagatatctt aatgaaatta
     9001 ataatcccaa agtcatactt ccaaagatag aaaataattg tactcatgta tggcatttat
     9061 ttgttttaca agttgaaaaa cgagatacat ttatggaata cttaaagaaa agtggtatag
     9121 agtctataat acattatccg ataccaccac atctatctga agcatatgct tatttgggat
     9181 ataaaaaggg gagttttaat atagctgaga actattcaga taaagttgtt agtatacctt
     9241 tatttattgg tatggaagat gaagaagtta gttatataat taaaattata aatgaatatt
     9301 aaactactca taataatatc aaaaataaat tttttatata tcatttattt aaatttcgtt
     9361 aattgacatt tttaaatatt gatatattgt acaaataaaa ttgaaatata tggaaaaata
     9421 tagaattaag atgagatttg tcttatattt ttataaatta aaaatatttt caaagagttt
     9481 attataaaat tgtaaactat agtgtttata atctttgtaa ttattggtaa ttaaatttaa
     9541 aaaataaaat atttaattat acacaaaaaa gatatttaaa aaatttgtta tttattgtat
     9601 acttaagtta aaactaaata ggcaaatcta gagaaatcta gtgacgcaaa gctataggga
     9661 ctaaggttta tatacataaa ctatgtcagc cagttgccaa aaagagtcct aggtgtattg
     9721 tatacctaag aaaagtctat aatgactgcc tttttggcag tcattttgtt atggaaaaat
     9781 cactttattt cagtagaaaa tacacataat aacatatttt accaattata acataaaata
     9841 aagaaaaatt atctcaaaaa gttactat
//
LOCUS       DAELTA010000137         9706 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.137, whole genome shotgun sequence.
ACCESSION   DAELTA010000137 DAELTA010000000
VERSION     DAELTA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.137"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            71..1501
                     /locus_tag="KPG13_002855"
     CDS             71..1501
                     /locus_tag="KPG13_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG0986555.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1604..2707
                     /locus_tag="KPG13_002856"
     CDS             1604..2707
                     /locus_tag="KPG13_002856"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0986556.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2722..2958
                     /locus_tag="KPG13_002857"
     CDS             2722..2958
                     /locus_tag="KPG13_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0986557.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            2975..4024
                     /gene="vorB"
                     /locus_tag="KPG13_002858"
     CDS             2975..4024
                     /gene="vorB"
                     /locus_tag="KPG13_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG0986558.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4026..4766
                     /locus_tag="KPG13_002859"
     CDS             4026..4766
                     /locus_tag="KPG13_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG0986559.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4769..5308
                     /locus_tag="KPG13_002860"
     CDS             4769..5308
                     /locus_tag="KPG13_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG0986560.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5432..7531
                     /locus_tag="KPG13_002861"
     CDS             5432..7531
                     /locus_tag="KPG13_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG0986561.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7798..8313)
                     /locus_tag="KPG13_002862"
     CDS             complement(7798..8313)
                     /locus_tag="KPG13_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG0986562.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8405..9616)
                     /locus_tag="KPG13_002863"
     CDS             complement(8405..9616)
                     /locus_tag="KPG13_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG0986563.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3620 a         1203 c         1817 g         3066 t
ORIGIN      
        1 aagattcaaa atatatatta ttatacgtga ataataaaaa gtaagtaaat tttataaggg
       61 aggggtagat gtgagtagtc aagaaagcaa accaaaagga aatcttatat caaattttag
      121 agatgatatg agagacccta agaaaagagc aatatacaca tattcaagtt ctataaattt
      181 agcgtacaat tttataggtt actttgtatc aactgttaca aatatttttt taactgatgt
      241 ggcaaaactt ggtgtaatta tggcaggtgg tatgcaaatt atacaatctt tcataaaaat
      301 tgctgtagct cctataacag gtacagtatt tgacaaacaa ccattcaaaa aaggaaagta
      361 ttatccatgg cttagatatg caccagcagc gttagcatta acatatggaa tatttttcat
      421 agttccatta atgaaaataa gtccagcatt acttttacct ttgatgacag ctttattatt
      481 aatagcatct tgtgctcagc aagtagtttt cactataatt tatgctattt accctgtagt
      541 tgctaagaca cctagagacc gagttatagg ctcaactact acgaatatat ttaaagaagt
      601 tggtaaattc ttagtaggtt tttcatatcc gttattatta gttttatttg cagatgtatt
      661 aggtggagaa ggaatgggtt atttaggtac ctacttattc tttgcagcag gatgcctttt
      721 agtattttgg ttttcttcaa cagagataat aaaatctgga gcagaagaag aagttcttca
      781 aaaagaagag aagccaaaga ttaaagcaag tgaaatggca aaagctttat tcactaatgt
      841 accacttatg atggcatttt gtttagaatt tcttatatgt ataagatcta taggattagg
      901 acctttggct ccatattatt ttaaatatgt aatagaagat gaacgtggat tagcagtatt
      961 tttatcaatc atgcctttgg taagtgtagc attcatgttt ttcgcaccag tgtttataaa
     1021 aatctgtaga gagcaaaaac tagcttcaat aatttctttt tctatatgtg cactatgcca
     1081 cttattggtt gcatggactc cttgggggaa aactacttta ggagtaacta tgttgttggc
     1141 aattggtgga ggtttttcaa atgtagtatc aataataaat cttaatttct ttgccggttc
     1201 ttgtgattat gggcattgga aatcaggaaa agacttacct ggcttaagta tgtcactata
     1261 tccagttgca atacaagtag gagtattact tgcaactact ataagaacag ttttaatgaa
     1321 ctcaatggga tatcaagctg atatggtagt cacagaagca gttaaatcag gttttataaa
     1381 tatgatttct tattctatgg caataccttt aataatagct gttgttatag caatactata
     1441 tccagtaagt gacaagaaat taaaccaaat aagagaagag ttaaatcaaa gaaatgctta
     1501 gttaaaagaa agttgagtag atggttaatt taagctatta aagtaagtag acgcaatagc
     1561 gtttatataa atgaaaatac atatacagag ggggctttta aaaatgatag aaaaattatt
     1621 gagaaatacc gtaaaagaat tacatcaata tgttccaggt gaaccaatag aaaaagttaa
     1681 ggaaaaatat ggtgtaaaag aaataataaa attagcttca aatgaaaatc ctctaggacc
     1741 atcaccaaaa gcaatagaag ctatgattga aatgttaaaa caaggtcaac tttatccaga
     1801 gccagaagct aacgaactta gaagaaaatt agctgaaaag ctagatttaa aaccagaaaa
     1861 ttttattgtt gctaatggtg cagataacgt tatcactcta ataggtgagg cttttataaa
     1921 tagaggagat gaagttatat attgtaaccc aacatttcca tcatatagaa ctgcaactat
     1981 aaaaaatgaa gggataccag tagaagtacc tttaacagaa gattataagt atgatttaca
     2041 aggtatatta gataagatta cagataaaac aaaattaata tgtgtatgta accctaataa
     2101 tccaacagga acaattgtgg atgataaaga gttagaagaa tttttaaaga aagtccctga
     2161 aaatattata actatattgg atgaagcata tatagaattt ttaacagttc ctaattatgt
     2221 ggatggactt aagtatgtaa gggaaaatta taacgttata gtaacaagaa ctttctctaa
     2281 aatatatggt cttgcagggc ttagagtagg atatggtatt gctaaagatg agataataag
     2341 aactttattt acagtaaaag agcctttctc tgcaaatcgt gttgctataa gtggagcaac
     2401 agcagcactt gatgatgaag agtttataaa agaaacatat gaattaaata gagttggaat
     2461 ggcttacttt aaagaagagt ttacaaaaat gggatttgat gttgtagatt cacaatcaaa
     2521 tttcctttat gtagatatga aaacagatat acctaagtta tttgaagact tgaaaaaaag
     2581 agggtttgta ataagaccaa gtgctacaca tgcacgtgta agtattggaa ctatggaaga
     2641 aaataggaaa tttgtagcag ttttaaagga aatattaaat attaaaggag agccaagtat
     2701 tggataatag atagaaatat tatgaaacgg ggtgtagata tgaacttaaa aataaacgca
     2761 gagagatgta aaagttgtga atactgcgtt atatcttgta aaaaaggagc attaaaaata
     2821 tcaagtaaga taaataaaga aggttatgct catgtagaag ttgatgagag taaatgcata
     2881 ctttgtggaa tttgctatca ggtgtgtcca gataatgttt ttgaaataat taaagaagaa
     2941 gcatgtcaag aggcttagaa ggagttggga taatatgaaa aagatgtgga aggggaacca
     3001 cgccattgca gaagcagccc taagaggagg ttgtgaattt tatgcaggat atccaattac
     3061 accacaaaca gaggtaatgg aatttttatc acatagaatg tctgaacttg gaagaacttt
     3121 tatacagtcc gaaaatgaga tggctgctat atatatggta tatggagcat atgcaagtgg
     3181 aatgaggtct atgacgagtt cttcaggacc aggaatatct ttaaaacaag aagggatttc
     3241 ttacttatgt gcaaatcatt acccttgtgt aatcgtaaat gtacagagat gggggcctgg
     3301 tcttggctca ttggactcag cacagacaga ctatctaaga gatactagag ggggaggaaa
     3361 tggagattat catttgatag tttatgcacc aaacagtatt caagagactg tagatttgat
     3421 gtataactca tttgatgtag cggagaagta cagagtgcct gtcgaaattt tgacagaagc
     3481 agctttaggt caaatgatgg aaccagtaga atttccagag tttaagaaga gagaaggaga
     3541 tttaggttgg acttatgatg gaagtaatag agaccatgca aaggttgcag ataaccaaaa
     3601 gccaactttt tgtatggaga aacgtatgag aataactgaa aatgaacaac aatgggaaga
     3661 ttatcaagta gaagatgcag aatatgtgtt tgtagcgttt ggtataccat caagaacaac
     3721 tatgaatgct gttagaagac ttagagaaca aggtgaaaaa gttggaataa taagaccaat
     3781 aacagtatgg cctttcccat ataaagcatt tgaaaaggta agtaaagatg taaaaggatt
     3841 tatctcagta gaatcaacag acacaggtca attagtagaa gatgttgctt tagcctcaaa
     3901 gaaagtatgt aaagaaaatg tacctgtgta tggtttattt agtggaaatc atataccaaa
     3961 aacattgcaa gtaatggaca cttatagcaa aataaaatct ggcgaaatca aggaggtgtt
     4021 ctaatatggc tacagaaaaa ttaacaccta gcgttgtttc aataccaaac aagttttgcc
     4081 caggttgtgg tcatggaata gtaaatcgta taatagcaga ggtaatagaa gaacatggat
     4141 atgaaaaaaa tcatgtatta acattaggag ttggttgtgt ctgcaatatg aattttagct
     4201 ggaatggtga taagatgcaa actgcacatg gtagagcttc atctacagct ataggagtta
     4261 aagtagcact tccagataca ttagtaatga cttaccaagg tgatggagat gcttatgtta
     4321 taggtctttc tgaaacttta aatacagcat atagaaatca taatgtgact gtatttgtaa
     4381 taaacaataa taattttgca atgactggtg gacaaatgtc atggaccact atgccgggtc
     4441 aagtcacaac aacaagtgta aatggaagag atataaagac tactggtagt ccactgaaag
     4501 ttcctgagat ggtggcaaac ttctttgatg tagcatatgt agcaagaggt tcagttcata
     4561 gcccaaagga aataattaat ctaaaaaaat atataaaaaa tgcaatagaa gctcaattaa
     4621 atggagaggg atattcctta gttgaaatat tagcaccttg cccaactaac tggggtgtat
     4681 cactagagaa atctatcaaa tggatggaag aagaaatagt gccatattat gctcttggcg
     4741 aatttaagca aagggatggt gaatagttat ggaaaaagag ctagtatttg cagggtttgg
     4801 tggtcaagga gttttaactc ttggtctgat tgtagctgag tcagcacttg aattggggaa
     4861 acaagtaact tggatgcctg catatggtcc aactatgaga ggtggaaaag cctatagtgt
     4921 agtaaagttt tctgatgaga gtataggtgg gcctgatatg gaagaaatag atgttttagt
     4981 agcaatgaat aaaccatctt tagattatat aaatttagtt aaagaaggtg gaacagtagt
     5041 tattaataca tctgcaatag atgagaatgt aacattaaga gaagatataa gtgtagttaa
     5101 aataaattgt caggaattag ctcaaaaagt aaacaatcca aaagcagcaa atatagtagt
     5161 acttggagca ttaattgcta aaactgggtt atttgaaaaa gaattagctt taaaaactat
     5221 gtgtgatttc tttgaggaaa aaggaaaagg aaagtttaat gttcaaaatg aagcagcatt
     5281 catggaaggc tataataacg ccatttgata aattaccctt atcttaagtg atttatctca
     5341 agatttaata atctttagtg ccaagagaaa cttcatcttg agataaaatt cacttaaaat
     5401 cacaactata aacatatagg agcgtgaatt tatgagtttg gaaaaactta ttagaccaaa
     5461 aagtattgct atagtaggag tgactgataa acttggtttt ggaagaagtg cagcacttag
     5521 tatagtaaaa agtaaggaaa cagatagagt atattatgtt aatcccaaaa gagaagaatt
     5581 gtttgggaga aaatgttata agacgataca agaagttcca gaagtagtag actgtgtagt
     5641 agtatgtacg cctagaaatg tagttccatc tgtattaaaa gattctggag agttaggaac
     5701 aaaggcagca gtggtatatg ctagtggatt tgcagaggaa ggtacagagg aaggtacaga
     5761 tttagaaaat caacttatag aaatatcaaa tacatacgat atgaaaatat tgggtccaaa
     5821 ctgcatgggg ttgctaaatt gtatagataa ggttaatcta tgggcaggag gttctaaatg
     5881 ggatttagat actaaaaaac ctggtatagg aattgtaggt caaagtggat ttataactgc
     5941 tgaaattgtt tcaagtgatt attttaatat atcttatggt ttttcaacag gaaatggaaa
     6001 tatagttaca ctagaagatg cagtagattt ccttgtagat gatgattatg catcagtaat
     6061 agcaatttac cttgaaggtt taaaaaatcc tcaaaaattt atagatgctt taaaaagggc
     6121 agcacaaaaa agaaagccag taataatatt gaaatcagga aggtcagaaa aaggtgctat
     6181 ttctgctgct tctcatacag gaaatttagc tggttcgagt aaagcttttg aaagtatttt
     6241 tgaaaaatat ggagtaattt ctgtggaaaa tttagaacag tttatgtgtt tagcacaagc
     6301 ttttagtgtt ttagatggta atttacctgc aaattctaat tttgcagcaa taaacttctc
     6361 aggaggagaa aatactattt gtgcagattt agcagaagaa aatggtgtag aacttgcaga
     6421 aatatcttct gaaactaaag aagaaatgga aaaatatctt ccaggatttg caacacctaa
     6481 aaatccacta gatgctacaa cagcattatt ccatgaaaag gatatgatag taggtttatt
     6541 acataccttt gaaaaggatg atagtgttgg atttactatg gtaggtgcaa atataagaga
     6601 tgaagaaaat gaaatgcatg aaaccctttg taaagctgta agtgaagcta gagaacaagg
     6661 attgaaaaaa cctgtgtttg cagttcctac acttgaagga aatagatatt tagattatag
     6721 gactagatta gaagataatc aagtgcctat aatgtcatca gttggaacta catttacttg
     6781 ttttaataaa atggctaagt ttatagatta tgattattca aaaagaactt tagaatttaa
     6841 agctgttaag aaaagagagt caaataatgt agtagcatta tcagaattag atagtaagat
     6901 tgagatgaaa aaatatggag taccagttcc aggacaaggt aatgcaaaaa gtattgatga
     6961 attagatgaa atcttaaaat atattaagta tcctgttgtt ttaaaaataa attcttctga
     7021 aatattgcac aaatctgatg ttggaggagt taaaatagga attaagaata gagatgaggc
     7081 agtagatgca tataatgaga ttttaactaa tgtaaaaaaa gcaaaaccag atgctaatat
     7141 tgatggtata ttagttcaag aaatggtaga aagtggtata gaaattatta ttggtataac
     7201 taatgatgac caatttggac caatgttatt agtcggttta ggaggagtat ttgtagaggt
     7261 atttaaagat actacattat atccattacc tataaatcat gatgaagcta ttatgatgct
     7321 taaaaaacta aaatcattta agcttttaaa tggctataga ggaagtgagc catgtgatat
     7381 tgatgctctt gcagatatga tggtcaagtt aggaaaatat gcttatgaaa ataaggatga
     7441 agtcaaggaa atagacttaa atccagtgtt tgtatatcct aaaggaaaag gcgtatgtgc
     7501 agtagatgcc ttaatagtta aatataaata gtttataagt tttggtattc aaccataatt
     7561 aaagcaccaa ctgaaaatca cattttgtaa gttagatagt gtgattttca gtcaccccaa
     7621 atttaattta aaaatatttt aaaatgaaag tatagtttta agtttcattg tgaaatagca
     7681 ctgtataaac aataaaatac tacgtataaa acgaataata aaaggtgttt taatgataag
     7741 aatcaatctt gattttcatt aaaacacctt ctttattata ttaaaaattt ttaaacgtta
     7801 tattgtcaca ggtgggtttg ttggagctgc atatgtacga ttagcaaatt cttggtcaac
     7861 aaagaatagc ccaccctctc cagcaagttt aacacgtttt atattgtctt cacaatttgt
     7921 ttcttcttct gcttgttcag atataaacca aagtaagaat tgagaagttc taaagtctga
     7981 ttcagagttt gctacagctg ctaagttgtt tattaaagct gtaacctttt tttcatgagc
     8041 taacgtacgc tcaagaatat ccattgcaga agtaaagtct gaatctggca ttgggatagc
     8101 atcaagctga acctttcctc ctacattatg taagtatttg taaaaaaaca tagcgtggtc
     8161 tgtctcttct ttatattgaa cataaaacca attagaaaat cctttaagtc cttctgcttc
     8221 aaggtaagaa gacatagaaa gataaagata cgcagaatat aattcatggt taatctgctc
     8281 attaagtaat ttttccattt tagctgaaat catttttata cctccaaatt ttaaatgctc
     8341 ttataaaata taataatatt aaatggaaaa atattcaata aaagtgttta aatattttac
     8401 atatttaata aaaatttata ctaccattaa aaaaattata acttacaatg gctatagagc
     8461 caattaatgg tgcatttgct ccaaaatttg aatgccttac acttattttt ttatatttag
     8521 aaaaacttgc tgaagcagtc aattttttta ataatgtatt ttctataaat gtaccaggta
     8581 tattaaagtc atagcctatt attatagaac tacaatcaat caatgtaagt gtattaacta
     8641 atgcatatga tacgtatgta caaaattcat caagcaaaaa catggcaaga ctatctttat
     8701 tatttgcctc atctattaac ttgactaaag aaaaatcttt acagttaatt aatggggagt
     8761 taggataaat atggctcaat gattcaattc ttttaattag attactgaca cttgtataat
     8821 aatctaaaca tccattgtta ccacaactgc acaatggtcc actaaaatta attgatgtat
     8881 gtcctatttc tccactttgt cctaggttgc cagtatgaag tttattttct agaactagac
     8941 cagctccaat accattcatt atgtgtaggt atatataatt tgatatattt ttacctaaac
     9001 catacatttt ttctgctaat gcacccgaat tagcatcatt tatcaaaaat attggcaaat
     9061 cagacatttc ttttataaaa tctactatat ttaggtttga tacatttcca aaatctgggg
     9121 gatttagtat ggtaccattt atatcatcta ctggacctat tgaagaaata ccaattccaa
     9181 ttatatctct cttgttgatt ttcattattt tagtaaacat tctttttatt atatctttta
     9241 aaacatcatt tgatagaaat ttagggtaag tttcagaaag ttgttttaca atattacctt
     9301 ttaaatctga aataattacc ttacatatat ttcttcttat taggattcca caaatacaag
     9361 gcgatatatt agaaatatct aataaaatag gtttacgacc tgatgaatgt gcatttaaag
     9421 ttgatgactc tatttcccta atcatatttt cattaataag ttcagtaact agatttgata
     9481 aagtcatttt agtaagtcct gtagacttag ctaaatcaac tctggacata ggtgtatttg
     9541 tagaaagtaa tttcaatatt attaacttat ttttaatttt tacatctttg atgttagaac
     9601 cttgtttgtt actcatcata aatctcctta aataaagtta agtgttttat ttattttcaa
     9661 gtatactatc accttataat agagtaacat ttttttactt gaaaat
//
LOCUS       DAELTA010000138         9705 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.138, whole genome shotgun sequence.
ACCESSION   DAELTA010000138 DAELTA010000000
VERSION     DAELTA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.138"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(361..1254)
                     /locus_tag="KPG13_002864"
     CDS             complement(361..1254)
                     /locus_tag="KPG13_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG0986564.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            complement(1479..3764)
                     /locus_tag="KPG13_002865"
     CDS             complement(1479..3764)
                     /locus_tag="KPG13_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG0986565.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            complement(3761..4441)
                     /gene="pgmB"
                     /locus_tag="KPG13_002866"
     CDS             complement(3761..4441)
                     /gene="pgmB"
                     /locus_tag="KPG13_002866"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG0986566.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            complement(4665..6548)
                     /locus_tag="KPG13_002867"
     CDS             complement(4665..6548)
                     /locus_tag="KPG13_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG0986567.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            complement(6806..7627)
                     /locus_tag="KPG13_002868"
     CDS             complement(6806..7627)
                     /locus_tag="KPG13_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG0986568.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            complement(7818..8171)
                     /locus_tag="KPG13_002869"
     CDS             complement(7818..8171)
                     /locus_tag="KPG13_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986569.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            complement(8213..9655)
                     /locus_tag="KPG13_002870"
     CDS             complement(8213..9655)
                     /locus_tag="KPG13_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0986570.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
BASE COUNT         3127 a         1723 c          916 g         3939 t
ORIGIN      
        1 ctagttgtta tttactttta agaaagaatc cttttttact tcattgattt caatttacaa
       61 gtaaaagtct ttctgaaaaa aaaatatata tatctctatt cactttttta taagtaatta
      121 taatttttat agactaacgg tcagccttaa gggaaaaggt gttctttaag tgcagaattt
      181 agataaagta aataatcctt ttataaataa ttccaagttt taacttaaat aatttgattt
      241 acagtataaa gtaaattttg aaagataatg atatcgattt aataattaca agtaaaagca
      301 tgtcaaaata aggctatgat tttaaaaatc atagccttat caataaaaat ttaatcaaag
      361 ttacatttca ctttgaattt cactgtctat taattgaaga ttatactgtt gaattaattc
      421 tattagacga gctgcatata tactattgcc attttcatcc ttcgcagtac tataacctgc
      481 cttttctaat gctttagctt gatatatata cgtttttgca tcaaatacac cattgttttt
      541 atacctttta tttgtggcta aaaattttgc atggtctttt atagattcat ttttatcatc
      601 atatattcta aatttatcat ctataattgt atctaaatgt tctttcgttt ctaaagtcac
      661 tgacttgcct ttccaataag cgtcagcctt tataccaaat agattgttat attgttttgc
      721 caaagttgac ttgccccagc tagattctat tatagcttgt gcaatagtta tagatggaag
      781 tattttataa tccttataat tttgtattgc ttctgtttta atttcatcaa taaacttcat
      841 atattttgta tcagacttca atctttctgg ttttatacca taatccttaa gattgtctat
      901 ataattatca accaatcttt tttgtctatt actaaattct aactcatcca aaatatcact
      961 taacttattt attttattat ttttagaaaa attcttaaca aataaatctg atacttcttg
     1021 tatctgtgaa tccttaacgt tttttaagtt atttttattt aacacagcaa caatggaagc
     1081 tacatacttc cagttaactt gtgcattatt tttacttgct aaatcagcat attttatata
     1141 cttactgaca tttatatcat ccttttttat ctctctagat gaatattcct tcactaatac
     1201 acctattaaa attgatatta tcagtattat aacaatgaat tttttattct tcataaatat
     1261 tgtcctccct tatactatct gctgaatata atatacctta cttttgtgac atattcttta
     1321 cgaaattttg aaaattttat gaagatttat gaaatatatt taattaaaag cactactatt
     1381 gttataatat atagttttct atcacacaaa atagttaaaa taaataaata attagatagc
     1441 ttattttaac tatttatata ataaaaatat tattaaatct atttatcaat ttttatacat
     1501 ttttctaata tatatttttt cccatataca ataattttta ttggtttacc ttttaaattt
     1561 tctattttaa ttaaatcact acatacatct acattaataa tattatctct ataatttata
     1621 ttgaaagaat aatttttcca ctcttttggc aacttagggt caaaagatag agtttcattc
     1681 catgttctca tgcctgcaaa tccatgtaca atggaaagcc atgaaccaga cattgaagtt
     1741 atgtgcagtc catcatttgt atcattattg taattatcta aatcaagcct agctgttctt
     1801 gaatataatt cataagcctt ttctaggtta tctatttctg aggcaataat agaataaata
     1861 ctagaagata aagaagattc atgtacagta tatttttcat agaagtcaaa atttctcttt
     1921 ttttcttctt tagtaaatct atttccaaaa tagtaaattc cctgtaatac atctgcttgt
     1981 tttataaaac acgacctaag aatcttatcc caagaccaat tttgattaag aggaaaattt
     2041 tcttttggta aatcctttac ttctacaaat tctttatcta aaaaattatc attttgaatt
     2101 attatttgta gttcctcatc atacagcaga tacattttat ttgatattat tttccaaaac
     2161 tcaacttctt ccctagtcac attatttctg tcaatacatt cattacactc tttctctaat
     2221 cgtaagatat tttctacagc ataatctaaa caccatttag ctatataatt agtataccaa
     2281 ttattattga tattgttttc atactcgttt ggacctgtta ctccatgtat catatacaaa
     2341 tctttttttg tagacaaatg aactctactt gcccaaaatc tagctatttc tattattaca
     2401 tctattcctt tttcttttat atatttatag tcacctgtat aatttgtata gttatatatt
     2461 gcatatacta tacttccatt tcggtggatt tcctcaaatg ttatctccca ttcattatga
     2521 cattctactc catcaaatgt taccattgga tataaagcac ctttaagacc tagttttttt
     2581 gcattatcct ttgcctgttc taactgattg tacctatatt ctaaaagatt tctagttacc
     2641 tccttatctg ctacacctag atatactgga acacaaaacg cttctgtatc ccaataagta
     2701 gctcctccat acttttcacc agtaaatcct tttggaccta tatttaatcg cgaatcttct
     2761 ccataatatg ttgaaaatag ttggaataaa ttatatctta taccttgttg gctactttta
     2821 tctccattta tttttacatc tgcactttcc catcttttat tccataactt tatatgttca
     2881 tttaatatat catcataact tttagatagt tctctattta aaatgtcttc acattttctt
     2941 aatagctcac tttccttata atctctagtt gaggttatag atacatattt ttctatactt
     3001 gcaacctcat tttttaaaac atccattgtt atcgaatttt cacagtaatt ataatcactc
     3061 tcatgagata cagcttctac atcaccattt gtcttaatat acatcattga acccacagtg
     3121 aatactggtg tattaaatgc attctccttt gttcgagaaa gaatactccc tttatattct
     3181 aaagaacttg aagaaattcc attccaaaac ttttcctcat agtttgaatc ttcatttttt
     3241 atatttgaat ctaaatatgg tacaaatttt attttagtat tagaatttaa tgattttata
     3301 ctgtatttaa ttactgcaag ttccttaaca gatatactta aaaacctagt aaccctaact
     3361 tcaaattttt tatcatttat atttacaata aacctacgat ttaaaatacc atttttcata
     3421 tctaattctc tatgaaaatc ttctacttta cattttgcta aatctaattc tacatcatct
     3481 atgaaaattc ttacacctat atagttgact gaattaggaa ttttaccaaa gtattctgga
     3541 tacccatttt tccaccaacc aacacgagtt ttatctggaa accatactcc agctatataa
     3601 gaccccttgt gtgtatctcc actataattt tcctcatagt ttcctctcat acccatatat
     3661 ccattgccta tactcattat ggactctgct aatctatttt ctttgatttc aatcttatct
     3721 tgtataatct tccactcatc tacattacta aataaacatt tcatataatt ctcctaacta
     3781 tatactcatt atacttttct tgtatatatt caaactttaa ttgatttgtt gaatctacaa
     3841 ctaaatttgc tttttttaag ttttcataat ttccaacacc aacagaaaac atattagctg
     3901 agtttatagc gtctatacct gcacttgcat cctctatccc tatgcaattt tgtggattta
     3961 cattaagccc tttcgctgac ataagaaata tctctggatg tggtttattg tttttacatt
     4021 ttcctgcatc agctataaaa tcaaatttgt cacttatacc caaatgattt aatacattta
     4081 ttgcattctt acttgcagaa gatagaccta tctttatatt attagatttt acatcaatta
     4141 acaagctttc tatgcctggc aaaatatcat ttgaagttat ctcatctatc aatgatacat
     4201 agtaattatt tttttcttca gccattctca ctttttcttc ttctgaaaat gaatatttct
     4261 tatttccaaa ttctaatatc ctgtccaatg actccattct acttatgcct tttaaagatt
     4321 cattaaattt tgtgtctata tctatcccaa ctttatgagc tagttttctc caagccatat
     4381 agtgataata tgcagtatct gtaattacac catctaaatc aaatataaat gcttcaatca
     4441 ttcttatttc ctctttctac aagaaatttt tagactaata atcaatttat tttctatctt
     4501 gtatttaaga tattatttat aaaattatct actctaactc agtctttaat ttaaaatcta
     4561 ctttcaattc actcacttgt agaccatatt ttttatcgtt tataaagact tgtttaaact
     4621 gtataataat ttaacatgaa atctaaaata aatacttttt tgtattattt taaaataatg
     4681 aacttgtatt tctctaacat tagttcttct tttaaatcaa ctatttcttc ttcatataaa
     4741 tcttttactt ttttatttgc taaataatct ggtaacgtca aaattatatt tttagatgaa
     4801 ttatttatta ttatagaaat agattctttt ttcaatataa tcgttccatt ttccttatct
     4861 gctaacaccc attcatttga tatagaccta agttctttat tttctttacg aagtctaata
     4921 atcttcttca taaaatcaaa tatatcctta ttccattttt cttcattcca ttccatacat
     4981 tttctttgac cttcagatat tgaacttaat tctccttcca ttccgatttc atcaccatag
     5041 tatatacaag gacatcctgt ttgtacaaac ataaacatat atgcaagttt aaatctatca
     5101 atatttccat tagcataagt caatattctt gtagtatcgt gacttccaag taaattaaaa
     5161 ataacttcac taacttgtat tggataactt accattacat catttatcat atacttaaat
     5221 tcttcggcgt ttatctcatc tgtacaaaag aattttttca tggcttctga catcaagtaa
     5281 ttcataactg aatcaaattg gtcacccatt aaccatggta aactaccatg ccaaatttct
     5341 cccaatatat aaatgtctgg ttttactttt ttgacttctt ctctaaactt tctccaaaaa
     5401 acatggtcta cttcatttga aacatctaat ctccacgcat ctatatcaaa ttcctgtatc
     5461 cagtacttgc ctacatccaa taaatatttt attacttctg aattttctgt attaagttta
     5521 ggcatatatt tttcacaccc aaaggtttca taaggtatat ttttttcatc tatttgttct
     5581 attggagtat caattttact catatctttt atataaaacc agtttttata tcgagaattt
     5641 tccttatttt gaactacatc ttgccactgt ttagaataat atcctatatg attaaaaaca
     5701 gcatccaaca ttatttttat atttctctta tgcgcttctt ctattagttt ttttaaagtc
     5761 tctttatctc ctaatgtagg gtctacctcc atataatcta ccgtatcata tctatgattg
     5821 gtttttccaa ttgtaatagg gcaaaagtaa attccgttta ttcccaaatc actcaaataa
     5881 tctaagtgtt ctatcacacc ttgtaaatct ccacctgtaa aattatctct ggttggaata
     5941 gctccccatt tatctactcc ttctgggttt atagacgggt caccatttgc aaatctatct
     6001 ggaaatatct gataccaaac agtatctttc acccaatttg gcactttagg tatatctata
     6061 taatttaaat atggatatcc aaaaaaatta cctatttcac ataattcctt ttcatcattt
     6121 tcatttccca actctattat tcttttttct gtaaataaaa gtttttcctc ttgaccttct
     6181 attataaagc catatcgtga gcgtttattt aaaggcttac acttagctat ccaatagtca
     6241 aataattcag tttctacttc tttactcatt actatatttg ttccaccaga ccaaccaact
     6301 ccagatgcat ttaaatttcc tcctcctgca cctcctttat cccacacata ttggtctcct
     6361 attcgtaaaa ttactctttt cgcttcattt tttttagttc taagtcttat atgtagtgtt
     6421 tctctatcat aaccataggc ataattgctc attggtatat gtaatatagc ttcttttgtt
     6481 aaactatttt gaattgttcc tttgatttga ttacttaaag ttatattttc tttattttta
     6541 ttagacattt taaaatttcc ctcctttata ataatttata taatctaaag ataggttaat
     6601 atattttact attatatcac aataaaaata tataatagta aaatatatca acaataaact
     6661 attcaattta aaataagcta tactatttaa atttaattct atttagttat tttagcaaaa
     6721 taaaaacctc tagtattttt ctagaggata caatactata actattaact ttttaactat
     6781 ctattaattt acttcaaaac acactctaag ctatattgtt agatttaact ttttctatat
     6841 ttacaaaata ggtttctctt tctttaaata aagatacata tttaaagtca agatacttaa
     6901 gcatatcata ggccacatca aaatctttgc atatatcttc taccctatga gaatcagaac
     6961 ctacagtaac tataaatcct ccaagttctt tatatctttt tagaatatct tcatttggat
     7021 gtatagcatt tagtccatat ctataaccag aagtatttag ctctatacca ctcccattat
     7081 tgattattga ctttaagacc tcatcaataa catctttaaa gtcattgtat ataactctat
     7141 tatctgaata tattccatac ctagatatat aatccaaatg accatatata ttgtaatctc
     7201 caaattcacg attagtttca agtatgctat taaaatattt ataatacgca tcttctttgc
     7261 taagaccttt aaaatacgcc tttgatggaa caggtacatt gtcaattgta tgagttgagc
     7321 acaatacaaa atcaaaagga ttagaattta ttatttcttc tatttcattt tttagatgtt
     7381 ttctgtaacc cacttcaaca cctagtaata actctatgtc ctttttatat ttttctttaa
     7441 acttattaaa tactggaaca tagtcattat aatttatgca aaatcctcta tcttcatcat
     7501 gttctaaatg gtctgtaaaa gtcaaatatc taacaccaat acttatagct ttttttattg
     7561 tatcttccat ggaatccttt ccatcacttg aaaaattagt atgaatatga ttatctatta
     7621 aattcatatt atccctccct ttattttatc tatcttaatt atacaacaag tttattgtat
     7681 acatttgtta aaagaaaatt ataatggtta aattttattt acatactttt atttttacaa
     7741 aaaagagaag cacctatttc tgattttaga aaatcattaa ggatacttct cctaatgtta
     7801 cttttatata cttcaattta atttttaaaa gtcttaattt tgtaatagag tttagctgtt
     7861 atatgtccaa tcatataaaa agctgtataa agtaaaacat atatatatgc agaatcattt
     7921 aaaaatatat acattaatac tatataagtt actaaagaaa caataagaga taaaatccaa
     7981 ttagtcttat ttaatacaca aattatccct tgtatcaaaa aagaaattgg aaaaatcaaa
     8041 accattccca caacaaaaat acttttagat tgaatatctg gaatattaaa tatatctgta
     8101 gcaaaagtta aaaacattag caccattatg actgtaggaa atatgatact aaaaatatta
     8161 ctctttttca taataatcaa tccttattta ttggatattt gataatcttc aattattttg
     8221 ttgcctcaat ttctcccatc attcttacta aatcttgtcc atattgacct ccagcccaat
     8281 tattagataa actttcaact gttttagcct taccaaataa agatttttca tgtctagggt
     8341 ctactggatt tgaaagtggg taaccatctt gtcctgcata taagcataaa tgttgaattt
     8401 gagctaatat tccttcttcc caagtatcaa atcttgaatg ggcattctta tcagtatcag
     8461 aaccacctac agaagtttta agtccacaag gattcttaaa tgatgcatct aatactccac
     8521 caaaattaca aaaaccagtt tcttttgcag attgagctac tgccaaaaca ggattaacac
     8581 cttcctgaac agcagtatca tataatattg gtattaactc tataaataaa tcattagctt
     8641 ttttagattt tgcccatttt aagcacgctt ctaaactagc tgttggctga cctaatatgc
     8701 ttgtctcatt tttcataggt gactcacctt ttgtagacga atcgccatta ttttgtgcag
     8761 cagaaacaca cttattatac acactgtcac ttattgaacc taatctagta atcttatctg
     8821 ctcctgctaa tatagactta tcactacttt ctgacaccaa tacaattgga ttttccttag
     8881 caattgtaga acctgtaagt gcatttacta aatcatatcc atcactcaaa taaaattctg
     8941 atgcatctgg ataaaactct tttataactt tcttatttgt atcatatcta tctgaaccac
     9001 caagtctttt agcattagtt ttattaacta aactagtgct cattgatata ctcccaccta
     9061 ctgcatatgt ttttacattg cttgttgaaa aaggcacact ctttccatct gtaagtacta
     9121 taggagctat atctcttact gatacgggtg cagcacttat tgtatctgct tctcctttat
     9181 atgcattagt taatatcact tcatcaactt ttttgatacc atttacctct ttagcaacac
     9241 tataactagt ctttattctg tcatctccac ccaatctagt tacctgtgcc cctgtgtttt
     9301 ttagttcagt ttctactgac ttacttattg ataattcttt acctattatg tatatctttt
     9361 taacattttt taatctttgt tttgtttcat ttggtatctt atttttttgt gcaagcaaaa
     9421 ttggagcatc aacagcacct gcaagaccac ttgctgaaag accatctgct atagaattat
     9481 ccatatttac cataattgct gtatcataac tctttttatc tgctattaat ccagccgtct
     9541 catatatatt gttaccttta attgtatctg atgttaatgc atctacatta ggtacattta
     9601 ttagaaatat agctaatgtt aaacctaatg ctaaaactct tctaattgtt ctcatattct
     9661 tcccccttga tatatcaagt ttaattcaaa aatgactata acata
//
LOCUS       DAELTA010000139         9649 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.139, whole genome shotgun sequence.
ACCESSION   DAELTA010000139 DAELTA010000000
VERSION     DAELTA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.139"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            349..1545
                     /locus_tag="KPG13_002871"
     CDS             349..1545
                     /locus_tag="KPG13_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG0986571.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1638..2162
                     /locus_tag="KPG13_002872"
     CDS             1638..2162
                     /locus_tag="KPG13_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG0986572.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2187..2363
                     /gene="rpmF"
                     /locus_tag="KPG13_002873"
     CDS             2187..2363
                     /gene="rpmF"
                     /locus_tag="KPG13_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG0986573.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2570..3127
                     /gene="fapR"
                     /locus_tag="KPG13_002874"
     CDS             2570..3127
                     /gene="fapR"
                     /locus_tag="KPG13_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG0986574.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            3138..4160
                     /gene="plsX"
                     /locus_tag="KPG13_002875"
     CDS             3138..4160
                     /gene="plsX"
                     /locus_tag="KPG13_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG0986575.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4186..5172
                     /locus_tag="KPG13_002876"
     CDS             4186..5172
                     /locus_tag="KPG13_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG0986576.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5281..6210
                     /gene="fabK"
                     /locus_tag="KPG13_002877"
     CDS             5281..6210
                     /gene="fabK"
                     /locus_tag="KPG13_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG0986577.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6226..7176
                     /gene="fabD"
                     /locus_tag="KPG13_002878"
     CDS             6226..7176
                     /gene="fabD"
                     /locus_tag="KPG13_002878"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG0986578.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7176..7925
                     /gene="fabG"
                     /locus_tag="KPG13_002879"
     CDS             7176..7925
                     /gene="fabG"
                     /locus_tag="KPG13_002879"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG0986579.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7979..8203
                     /gene="acpP"
                     /locus_tag="KPG13_002880"
     CDS             7979..8203
                     /gene="acpP"
                     /locus_tag="KPG13_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG0986580.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8232..9470
                     /gene="fabF"
                     /locus_tag="KPG13_002881"
     CDS             8232..9470
                     /gene="fabF"
                     /locus_tag="KPG13_002881"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG0986581.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
BASE COUNT         3808 a         1056 c         1958 g         2827 t
ORIGIN      
        1 tagaaatgtt acctacataa aaaactggtt aaatgttatt aaaataactt tattagctct
       61 ttatattata ttattataac aaatgggaaa gaataatcta cattaagtta acttgttaat
      121 atattattag tgaaatacac aatttaccat actagaaata tgatataata taaaaaaaaa
      181 gtatatactt ttatattaaa aggtgatata ttatttatga aaatcgttta atgtatacaa
      241 taatatatct ataacaaaat aataaaagat agagttttat ttagacattt attacaaatc
      301 aaaatttatt acatattatt tataaaaaaa tctaaggagg aaataataat gaaaatatta
      361 gtattaaact gtgggagttc ttcattaaaa tatcaattaa tagatatgaa taatgaagaa
      421 gttttatgta taggattggt tgaaagaata ggaatagaag gttctatatt aaaacatgaa
      481 aaagcaggta gagatgataa atacgttgtt gaacaaccaa tgaaggacca caaagatgca
      541 atagcattag ttttagaagc tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa
      601 gaaatagatg cagtaggaca tagggttgta catgctggag aaaaatttgc aacttcagta
      661 gtaataactc cagaagtaga agaagcttta aaagaatgta tagatttagc tccacttcat
      721 aatccagcaa acataatggg aatagatgct tgtaaagcta tacttccaga tgtaccaatg
      781 gtaggtgtat ttgatactgc tttccatcaa acaatgccta aatcttcata cttatatggt
      841 ttacctcatg agctatatac taaatatggg gtaagaagat atggattcca cggaacttct
      901 cataattatg tatctcaaag agcagcagag atattaggaa aagatataaa agatttaaaa
      961 atagtaactt gtcacttagg aaatggtgct tctatagctg ctgttgatgg tggaaaatgt
     1021 gtagatacat ctatgggatt cactccatta gaaggattaa taatgggaac tagatgtgga
     1081 gatatagacc cagctatatt accattctta atgagaaaag aaggtttaga tgctgatgga
     1141 ttggataaat taatgaataa agaatctgga gtatatggaa tgactggaat ttctagtgac
     1201 ttcagagata tagaagatgc agctaaaaat ggagacgaaa gagctcaagc tacattagaa
     1261 gcgtatgtta aaaaagtaca aaaatatata ggcgcttatg ctgctgaaat gaatggattg
     1321 gatgttgtag tatttactgc aggcgttggt gaaaatggaa aggctataag agcagatatt
     1381 gcttctaaca tggagttttt aggaatgaaa ttagataaag aagctaatga tgttagagga
     1441 aaagaaacag taatatctac tgctgattct aaagtaaaaa tgcttttaat accaactaat
     1501 gaagaattaa tgatagctag agatacatta agattggtaa aataattaaa ttaaacctat
     1561 gcctatcaag taaaaatact tgacaaacaa atgctagatt tgtataataa atttggtgat
     1621 atgttgaggt gaacttaatg aaagttagca tagaaaaaat aaatagaaaa gaaactgaca
     1681 aaatagactt gaatttttgt gaaaaaattg atactattag ttattgtgat gagatttaca
     1741 aattagtatc acctgtaaat ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc
     1801 tagatataga tgtcaatttt acaattgttg acaattgttc aagatgtctt aaagaagtag
     1861 aaataccact agagtattct atacaaggtt ttttagtaaa agaagaagat tatgatgaag
     1921 atgagtttga ggaatttgat ccttttatat tcgatggtga agaaatcgac cttattgata
     1981 taatagaaca gacattagat tttaatgtac ctcataaagt tctttgtagt gaaaactgta
     2041 aaggtctttg tcaagtatgc ggggcaaact taaatgaaga agagtgttct tgcagtgaaa
     2101 ttacaaacga tgaggaatac atagatcctc gttttgctaa attaaaagat ttatttaatt
     2161 aaattttgac taaggaggtg gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa
     2221 acacaaaaat gagaagagca gctaactcaa agatggaagc aacaggattt gtaagttgcc
     2281 cacaatgtca tgagccaaaa ttaccacata gagtgtgtcc agactgtgga tattataaag
     2341 gtaaagaagt tgtgtctaag taatagcttc taaaaagcat gtagagtata ctctacatgt
     2401 tttttttgta catataaaaa aataagataa tttactagag aaaataagtg gattttatgc
     2461 atattaaaaa atagaaaaat aataatataa gtattgaaaa tttttgtgtg ttactatata
     2521 ctactattag tagctggtac taattacagc tataaaattt ggagggaaca tgaaaaagaa
     2581 aagtaaagcc caaagacaaa aagagcttat agatatgcta aagacagatc ctttttatac
     2641 tgatgaagaa ttatcaagtc tgtttgatgt aagtatacag accataagat tagatagaat
     2701 gagtctaaac ataccagagc taagagaaag agtcaaatca atagcagaaa ctcagagttc
     2761 aaaagtaaag actttaggag taaaggaaat tacaggagaa atcattgatt tatctgtagg
     2821 gcgattaggt atatctatgt tagaagttac acaggacatg atttactcta agactaatac
     2881 tttaaaagat acgtatattt tttctctagc agattcattg gcaatggcta taatagatgc
     2941 acctaaggtt attatgagag ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga
     3001 taggcttata gcaaaagcag aagtgtatag aaatatagat aaaaagcatt atgtaaaagt
     3061 tgttatcaac aataaagccc aagagcaaat atttagagga aagtttatct ttgaagaatt
     3121 agattaggag ggaaattatg aaaatagtaa ttgatggaat gggtggagat aatgcaccta
     3181 aatctaatgt agaaggcgca gtaaatgcta taaaagaata tcaggttgat ttaataatca
     3241 caggagataa agatttactt gaaaaggaat tttcaaatta tgaatttgat agaaataaat
     3301 tagagatagt tcatactact gagattatag aaaacgaaga taaaccagta aaggctataa
     3361 gaagtaaaaa agattcatct atggtagtag cattaaatct tgtaaaagaa ggaaaggcag
     3421 atgctataat atcagctgga aatacaggag ccttacttgc tggtggatta tttgttgttg
     3481 gtagaataaa aggaatcgat agaccttgct tatgttcagc aataccaaat gtaaagcgag
     3541 gaatgacact tatagcagac tgtggcgcaa atgcagattg taaacctaag aatctagttg
     3601 aatttgcagc tatgagtaat atttattcaa gaaaagtttt aggtcttgaa aatcctaagg
     3661 tagcattagc aaatgttgga ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat
     3721 atgaagaaat taaaaaatta gatttaaatt ttatagggaa tgtagaagct agagaagtta
     3781 taaatgcata cacagatata atcatatgtg atggatttac aggtaatata ttacttaaat
     3841 ctgctgaagg tgtagcactt tcagttatga gtcttataaa ggaaactttt atggccagta
     3901 caaaaagtaa aataggagca ttacttataa aggatgattt aagaaaatta aaaagtttta
     3961 tagattattc tgagtatggg ggagctccac ttttaggtct aaatggagga gttataaaag
     4021 ctcatggtag ttctgatgct aaagctataa aaaatgctat aaatcaagga ataaaatttt
     4081 caaagggaaa agtcgttgaa gatattaacc aatttataag caaatacaat gaagaaaata
     4141 aaaataacga agatgaataa actcgttttg gaggtttaat atataatgaa tacaaaggct
     4201 ggaatattag gagtaggaag ttatctacct gagcaatcat atgataattt tcacttcgaa
     4261 aaaataatgg atacatcaga tgagtggata agtactagaa ctggtattaa ggaaagaaga
     4321 tttgccaaag agtctgaagc aacttctgac ttagcatcta aagctgcctt aaaagctata
     4381 gaatgtgcaa agttaaacgt agaggatata gagctgatta tattagctac aattactcct
     4441 gatatgtctt tgccttcaac tgcatgtata gtacaagatg caataggagc agtaaatgct
     4501 actgcatttg atatatcagc agcttgttct ggatttgtat atggagtaac tattgcaaag
     4561 caatttgtgg aaacaggttg ctataaaaat gtgcttgtaa taggagcaga aacttgttct
     4621 aagtttttaa attatgatga tagaacaaca gcagtattgt ttggagatgg agctggagca
     4681 gctgttattg gccctgtaaa tgagggtgga atactgtcta cacatatggg ctctgatggt
     4741 aaaggaaaag attgtttaaa agtacctgct ggaggttcaa gacttaaggc aagtaaagag
     4801 actgtagaag ctaatctaca tacaatagaa atggcaggta gtgatgtgtt taaatttgca
     4861 gttagaaaga tggcagaaac ttctttaaga gctcttgaaa aagcaaatct aaatacaact
     4921 gatatagact acttagtgcc acatcaagct aatataagaa tcatacaagc ttcttctaag
     4981 agattagagc ttgatatgaa aaaagtttat gtaaatatag acaaatatgg aaatatgtct
     5041 gctgcatcta taccagtagc attagatgaa gcttatagag aaggtaaaat taaaaagggt
     5101 gataatgtag ttttagttgg ctttggtgga ggtctaactt ggggggcttc agttgtcaag
     5161 tggactttat agtataaaat atctaaaatg atttaattaa caaaattaaa cttatacaca
     5221 aaataaagtt gattaataat aaaaagttta cttttatgat ttcaagtgaa ggagaattgt
     5281 atgaataaaa tttgcaaaat attaaatata aaatacccag ttatccaagg gggaatggca
     5341 tgggtagcta ctgcatcatt agcaagtgct gtatctaatg caggaggact tggcataata
     5401 gcagcaggaa atgcaccaaa agaagctata aagaaagaaa ttgttgagtg taaaaaatta
     5461 acagataagc cttttggagt aaatgtaatg cttatgtcgc catttgttga tgatataatt
     5521 gatttgatta tagaagaaaa agttcaagtt attactactg gtgctggaaa tcctgcaaag
     5581 tatatggata gattaaagga agctggaaca aaggttattc ctgtagtacc tacaatagct
     5641 ttggcacaaa gaatggaaaa gctaggagct acagcagtaa tagcagaagg tactgaaggt
     5701 ggaggacata taggagaact tactactatg gtcttagttc cacaagttgc tgatgctgta
     5761 aacatacctg taatagctgc tggaggaatt gtagatggta gaggaattgc agcatcattt
     5821 gcattaggtg ccagtgcagt tcaagtagga actagattta tttgcagtga agagtgttct
     5881 gtccattcaa actataaaaa cttagtacta aaagcaaaag atagagatgc aattgtaaca
     5941 ggaagaagta ctggtcatcc agtaagaaca ttaaaaaata aactatcaaa agaattttta
     6001 aagatggaac aaaatggagc tactcctgaa gaactggata aaaaaggtac aggagcttta
     6061 agatttgcaa cagtagatgg agacatagaa aaaggttcat ttatggcagg tcaaagtgct
     6121 gctatggtaa aagaaataac accttgtaag gaaattatag aggctatggt aaatcaagca
     6181 agagagatta tgccagcaat agaactgtaa atcaggagga gcaagatggg aaaagttgca
     6241 ctagtattcc ctggtcaagg agctcaatat gtaggtatgg ccaaggattt atatgaaaat
     6301 aatgctgttg ctaaaagtgt aatagatgag gctagtgatg ccttaaatat ggatttaaaa
     6361 aaccttatgt ttaatggaaa tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgct
     6421 atggttacac atagtgtagc agtattaaaa gcggtacaag ctcaaataga tttaaaatat
     6481 aatgcatgct taggattatc cttaggagag tatagtgcgt tggttgcagc agatgcaatt
     6541 gactttaaag atgctgtatg tttagttaaa aaaagaggta agtttatgca ggaaacagtt
     6601 ccagcaggtg ttggagctat ggcagcagtt ttaggtttag atagaaatgt acttgaaaat
     6661 gtggttaaag atatacaagg aggtattgta gaagtagcaa attacaattc tcctggacaa
     6721 atagttatat ctggtgaaaa tgaaaagata gaagaagcaa tggtaaaatg caaagaagca
     6781 ggagcaaaaa gggcagttaa attaaatgta agtggacctt ttcactcatc aatgttaaaa
     6841 gaagctggtg taaaattggc cagtgaatta gaaaaagtta acataactaa acctaaagtt
     6901 gatgtggtgg caaatgtaaa tgcagattac tataaaaacg aaggtaaaga cctgcttata
     6961 aaacaagtaa gttcatctgt tctttgggaa gattctatag aaagactttt aaatgatgga
     7021 tatgatactt ttatagaaat gggacctgga aaaactttaa aagcatttat taagaaaata
     7081 gcttccaata aaaaagcaaa tgtcaatatt tataatatag atggtataga ttctcttaat
     7141 gaatttgtac aaaattatag aaatggagag atataatgat aaatcttaca ggacaagtag
     7201 cagttgttac tggtggttct aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt
     7261 ttggagctga tgtagtaatc aattatactt ctaaagaaga tgaagcacta aaaactaaaa
     7321 atgaaataga aagtatgggg gtaaagtgta cctctataaa atgtgatgtg tctaaatttg
     7381 atgaagtgaa tcaaatgata gattctgttg taagcgaatt tggaaaaatt gatatattgg
     7441 ttaataatgc aggcataact aaagatggtc tgcttatgag aatgaaagaa gaagattttg
     7501 atagagttat agatataaac ttaaaaggtg tctttaattg tacaaaagca gttactaaac
     7561 ctatgatgaa aaagaagtat ggaagaataa taaatatgac ttcagtagtt ggaattatgg
     7621 gtaatgcagg gcaaactaat tattgtgcat caaaagcagg tgtaattgga tttacaaaag
     7681 cttctgcaag agagttagca tcaagaaaca taaatataaa tgcagtagca cctggattta
     7741 tagaaacaga tatgactaaa gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca
     7801 taccaaagaa atcttatggt aaaccagaag atgtagccaa tgccgtagca tttttagtta
     7861 gtgacatgtc aagttatata acaggacaag taataaatgt agatggtgga atggtaatgc
     7921 aataataatt taaaaagtag agaaatctat taaaataaaa aattttagga ggtacacaat
     7981 gtttaataaa gttgtagaaa taataataga gcaattagga gtagaagata aagaaataac
     8041 tatggaaaca tcattaatga aggatttaga agcagattca ttagatgcag tagaaataat
     8101 aatggcactt gaagatgaat ttggtataga aataccagat acagaagcag aaaacttcaa
     8161 atctatagga gatatagtta agtatataga agctaataaa tagtcgagct ttaaaaaagt
     8221 ggaggtatcc gatgaataaa agagtagtaa taacaggact tggatgtgtt acgccgttgg
     8281 gaacaggaaa agaagagttc tggagtaata taaaatctgg agtatctggt atagataaaa
     8341 taactaattt tgatgctagc acatatcaaa ctcagatagc aggagaagtt aagaattttc
     8401 atccagaaga atatataagt aaaaaggaat tgaagagatt agataaattt gctcaatttg
     8461 caatagtatc tgctaaatta gcagttgaag atgcaaatct tgatttagat aaagttgata
     8521 gagaacgttt tggtgttata ataggttctg gaataggtgg agttgaagct atagaaactc
     8581 aacataaaat tcttttggaa aaaggaaata aaagagtaag ttcacttttt gtacctatga
     8641 tgataggaaa tatggcagct ggtcaagttt caatattttt aggagcaaaa ggacctaaca
     8701 ctaatgtatg tacagcttgt gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa
     8761 ttcaaagagg tgatgcagac cttatggtag cagggggttc tgaagctgct gtaacaggtc
     8821 ttgcttttgc tggattctgt aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa
     8881 ctgcatcaag accatttgat aaagacagag atggttttgt aatgggagaa ggagcaggta
     8941 ttgttatctt agaagactta gaacatgctt tagcaagagg tgctaaaata tatgcagaag
     9001 tagttggata tggtttaact gctgatgcat accatatgac aactccagct gaaaatggag
     9061 agggagcagc taggtctatg aatatggctt tgaaagatgg aaatgttcca ttagaagaag
     9121 tagattatat aaatgctcat ggaacttcaa cttattacaa tgatttatat gaaactatgg
     9181 ctataaaaac tgtatttgga gaaaaggcat atgacttatg tgtgtcatca acaaaatcta
     9241 tgactggtca cttattagga gcatcaggtg ctatagaggc tgttgtttgt gctatgagta
     9301 tagaagatag ttttgtgcct ccaactataa acatacaaga agttggtgaa gatttggatt
     9361 tagattatgt tccaaatcaa ggtaaagaaa aaaatattag atatgcacta tcaaattctt
     9421 taggatttgg tgggcataat gctacaatag tactaaaaaa atatgtataa ataaatatta
     9481 aatataaaac taaaaaacaa gcacttgttt taactgtata aatttatatt agcttaaatt
     9541 taaagtctcc taaattattt taggagattt ttttattaag atttaagtct aggatatgtt
     9601 gtaaatcatt ttgatatatt gtattattaa ttattaagag gttatttat
//
LOCUS       DAELTA010000140         9647 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.140, whole genome shotgun sequence.
ACCESSION   DAELTA010000140 DAELTA010000000
VERSION     DAELTA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.140"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            399..857
                     /locus_tag="KPG13_002882"
     CDS             399..857
                     /locus_tag="KPG13_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0986582.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            918..1193
                     /locus_tag="KPG13_002883"
     CDS             918..1193
                     /locus_tag="KPG13_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0986583.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1252..2526
                     /locus_tag="KPG13_002884"
     CDS             1252..2526
                     /locus_tag="KPG13_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG0986584.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2656..3708
                     /locus_tag="KPG13_002885"
     CDS             2656..3708
                     /locus_tag="KPG13_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0986585.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3708..4739
                     /locus_tag="KPG13_002886"
     CDS             3708..4739
                     /locus_tag="KPG13_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG0986586.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4831..5643
                     /locus_tag="KPG13_002887"
     CDS             4831..5643
                     /locus_tag="KPG13_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG0986587.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5646..6566
                     /locus_tag="KPG13_002888"
     CDS             5646..6566
                     /locus_tag="KPG13_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG0986588.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6741..7187
                     /gene="rpiB"
                     /locus_tag="KPG13_002889"
     CDS             6741..7187
                     /gene="rpiB"
                     /locus_tag="KPG13_002889"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG0986589.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7217..7882
                     /locus_tag="KPG13_002890"
     CDS             7217..7882
                     /locus_tag="KPG13_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG0986590.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7992..9494
                     /locus_tag="KPG13_002891"
     CDS             7992..9494
                     /locus_tag="KPG13_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG0986591.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
BASE COUNT         3917 a         1061 c         1856 g         2813 t
ORIGIN      
        1 ggacaattct ataaaaattt ttcctatatt tcttataaaa tatagaatat ataaaaaata
       61 tataaaaata aattaaattc ataataaata attttataaa atagttgtta aacataaaaa
      121 accatttgta aaaaacaaaa aaatcatttc tctaacaaaa aaaaccacaa aaaatataaa
      181 aaagtatggc tagttaagtg attaacaagt aaaaattaaa ataaaaatca aaaaaaattt
      241 atcaatgaaa gtcagctaaa atcctggaag agagcattat ttttgggaaa acattttctt
      301 ttgtttcaca aaaaaagcca ctttttggca cacaacatgc tttatataaa gtcaagaaaa
      361 actaaggtat aagacaaagg caattaggag gtgtaaatat ggagtttata aattttgtaa
      421 gtgataaagt tatagacctt aaaatggatt gtaaaacgaa agaagatttc tttaaagaaa
      481 tacataataa agtttttgat ttaggctttg taaaagaaga atttggagaa aaaatattag
      541 caagagaaaa tgtatttcca acaggtttaa atttaggtga ttatggagta gcaataccac
      601 atacagatgc ggaatatatt aaagagcaat ttatatcagt ctgtactttt aatgaaccag
      661 tagtatttag ttctatggaa gaccaagatg aaaaagtacc tgtaaatttg gctttcgtat
      721 taggacttaa tcaaccacac agtcaattat ctgtattaac agaattaatg ggaattatgc
      781 aaaataaaga gttagttgat aagttgatga gttctactga taaagaagaa gttttaaaga
      841 cactaaagtc tttataaaaa taaaaaagta ctaaaaataa aaaatataaa aataaaaatt
      901 attaggagga aataaaaatg aaaaaaatat tagtagcatg tggagcagga atagcaacat
      961 caacagtagt ttgtgacaag gtagagagat tagttaagga aaataatata caagcagaag
     1021 ttatacaatg caaaatagct gaatgtgcaa caaagcaaga aggagcagat ttaatagtat
     1081 caacaactat actaccaaca acttataata taccagcaat aaaagcaaca gcatatataa
     1141 ctggagtaaa tacagcagca ttagataaaa aaatattaga tgctttaaaa taatcctatc
     1201 atagatacat tgaaataata ttttataaaa cagaaaaagg aggatttata aatggataaa
     1261 ttattagcag tagtacaata cattttaaat atgggaccga cagtaatact accaataaca
     1321 attgtagtac taggaatgat atttagaatt aagtttacaa aagctttaaa gtcaggtctt
     1381 acaattggta taggatttgt gggtataaac ttagttgtaa acctattaac atcaacacta
     1441 gggccagcgg ctcaaggaat ggttgaaaga ttcggactta atttaacagt aatagatgtt
     1501 ggttggccaa caacagcatc agcaacatgg gcttcaccag ttgcaccaat attaatacca
     1561 gtttgtttat tagtaaactt actattatta tacttcaata aaacaaagat attaaacgtt
     1621 gatatatgga attattggca ctttatagta gcaggtgcag ttggatatat agtaacagga
     1681 agttatgttt gggctatatt ctgtgcaata ctaatggagt tattagtact gtttattgca
     1741 gaaaagacag caaaaaatgt tcaggagttt tatgatttag aaggtatagc attaccaact
     1801 ggttcaacag cttcatttgc tccacttgga tatttagtag gtaaatttgt tgaaaaaata
     1861 ccaggaataa ataaaataca agcagaccca gactctatac aaaaaagatt tggtatattt
     1921 ggagaaccaa tgatgatggg gattatacta ggtataatac ttggagcact tgcgggatat
     1981 gatgtagcag gaatattcaa tgtaggtata tcaatgggtg cagtaatgtt acttatgcct
     2041 agaatggtta gaatattaat ggaaggttta attccaattt cagaatcaat aagagatttc
     2101 ttacaagcaa aatatggaga cagagaactt tatataggtc tggatgcagc agtagcaact
     2161 ggacatccag cagttatatc aacagctctt atacttgttc ctataacttt gttcttagca
     2221 gttatactac caggaaacaa agttttacca tttggagact tagcgacaat accattttat
     2281 atggcattta tagtgtgttt tagaaaaggt aatatagttc aatcagttat aacaggaaca
     2341 atagtaatag ctatatcact ttatatggct acaaactttg cagatgttca tacacaatta
     2401 ttacaacaag caaatatgat gccagaagga acaacaatgg ttacaagtat agatacaggt
     2461 ggaaacttgc taaaatgggg aatattgaaa ttatctcaat tagtttcttc atttggaata
     2521 ttctagtatg tagttaatag agattactct tttttagtaa agtatgcgaa aatttaacat
     2581 ttagcagcta ctaaaaaaga gtaatatttt ttataaattt ttaaaaatac aaaatacaag
     2641 aggaggagat ttatcgtgaa agcagcagta ttacatggca caaatgatat gagatttgaa
     2701 gatatagaaa taaaaccatg tgaaagtgat gaagtaaaaa taaaagttat ggcagcagga
     2761 atatgtgggt cagatcctcc aagagtatta aaacattgga aatatccagt tccagctata
     2821 ccaggacatg agttttctgg agtaattgct gaagttggta aggatgtaaa aaatgtaaaa
     2881 gttggagata gagtagttgc aattcctttt ataccttgta atgaatgtga atattgtaaa
     2941 agaggattat tttcattgtg tgatgaccat ggaatgttag gagctaaaag ttttggagct
     3001 tttgcagagt atgtaaatat aaaagctaca aatgtacttc caataggaga tatgaacttt
     3061 gaagatgctg caatgataga gccactagca gttgctatgc atggagtact taatataggt
     3121 gttcaagttg gagacactgt agctgtaatg ggtagtggaa caatggggca gttggtaata
     3181 caaggactta aaatagcagg ggcaggaaca attatagcag ttgatatatc tgataataaa
     3241 ttaagagaat caaaggaatt aggggctgat ataataatta atgctaaaga tattaaccca
     3301 gtagaaaaaa taaaagaact tactggtgga aaaggtgttg acatagctct tgaatgtgca
     3361 ggttcaaaaa taactcaaga acaatgttta cttataacta aaaagaaaag taagatagga
     3421 tttttaggga tagcatattc agatataact ttatcagaag aagcatttga aaatatattt
     3481 agaaaagaac tagaattaaa aggtttttgg aactcttatt ctgcaccatt tccaggacaa
     3541 gaatggacta agggaatcaa tctagttaat gaaggaaaga taaaattaaa agaaatggta
     3601 tctcatagat tctcacttga agatacttac aaagcatttg aaatgataag agatagaaaa
     3661 gaagaattta acaaaatatt aattttacca caaggggttg aaaaataatg aaagcagtag
     3721 ttaaaactaa accaggctat gataatgtag aagttttaga agtagaagaa ccaaaagcta
     3781 caggagacaa ggttaaaata aaagtagaat atagtggtat atgtggttca gatatacatt
     3841 catttaaagg tgaatatgca aatataaaag ccccagtaac attaggtcat gaattttctg
     3901 gaattgttgt agaagtagga gaagatgtta aaaatataaa agtaggagat agagttacat
     3961 cagagactac ttttgcaact tgtgagaaat gtatatattg tgaaactaaa gattataact
     4021 tatgcccaac tagaaaaggt attggtactc aagtaaatgg aagttttgct gagtatgtat
     4081 tatcaagaga agaaagcata catgtacttc ctgaaaatgt atctttatta tcagcagccc
     4141 ttacagagcc actagcttgt tgtgttcatg cagctcttga aaagactact atagaaagtt
     4201 ctgatacagt tttaataata ggaccaggac caattggttt attattagct caagttgtta
     4261 aatctcaagg tgctacagtt ataatgtctg gtgttacaaa agataaggaa agattagaaa
     4321 tagctaaaaa tcttggagta gatagaactg taaatgtaat ggaagaaagt ttggcagatg
     4381 tagtgaatga aatgactgat ggaaatggag taaacaaagg atttgattgt tctgggttta
     4441 tgccagcagt taacgaagcc ttaagactgc ttagaaaaaa aggtactttc gtacaagttg
     4501 gaatctttgc taaaaaatta aatgaaatgg accaagaagc aataatccaa agagagttac
     4561 aatatatagg aagcaggtct caaaagccaa gttcttggat tacagcatta gatttaatgg
     4621 caaatgggaa agtaaataca gaagctttag ttactagaac tgttggttta gacgaattta
     4681 aagaaggtat tacagcttta atgaacgggg aagaaataaa agtagcagta aaatcttaaa
     4741 aaaacatttg tattatttta aaaaaaattg taaaatctac tgtaggataa aaaaatgtaa
     4801 ggtcttaaaa aataacagga ggaagaaagt atgagcactc ttagagatca ttctaataat
     4861 attagaaaaa acataataaa aatggttgca gaagctaaaa gcggacatcc aggtggttca
     4921 ttatcaatag ctgatatgtt gacagtatta tattttgata agatgaacgt atgtgcagaa
     4981 aatccaaaaa tggctgatag agatagattt gttctatcaa aaggtcatgc agcaccagct
     5041 ttatatgcta cattagcaga aaaaggattt ttcccagaag aagaattaat aacactaaga
     5101 aaatttggtt ctaagttaca aggtcaccca gacatgaaaa aagtagcagg aatcgatatg
     5161 tctactggtt ctcttggtca agggctatct gcagcaaatg gtatggcact tgcaggaaaa
     5221 ttagataaca aagactatac agtatatact atattaggtg atggagaaat ccaagaaggt
     5281 caaatatggg aagctgcaat gacagcatca cattacaaat tagataattt aattgcattt
     5341 gttgacttaa atggattaca aatagatggt tctaatgaag aagtaatgaa tgtaagtcca
     5401 gtagatgaca aattcaaatc ttttggatgg aatgtaatag taataaatgg acattctttt
     5461 gaagaaatag gaaatgcagt tgatgaagct aaaaaagtta caggaaaacc aactgtaata
     5521 atagctaaaa cagtaaaagg taaaggtgtt tcatttatgg aaaataatgc tgcatggcat
     5581 ggaacagctc caaatgcaga acaagcagag caagcattga aagatttaga aggaggtcta
     5641 taggaatggg aatagcaact agagaagctt acggacaagt gttaaaagaa cttgcagaaa
     5701 ataaagatat agtagtatta gatgcagatt taggaaaagc tacaaaaagt atatcattta
     5761 aagaagttgc cccagataga ttttttgaca tggggatagc agaaggtgac atgattggta
     5821 cagcagcagg tcttgctact tgtggaaaaa taccttttgc gagtactttt gcaatatttg
     5881 cagcaggaag aggctatgag caaataagaa actctgtagc atatccaaat ttaaatgtaa
     5941 aaatagcagc tactcatgca ggtgtaacag taggtgaaga tggtggtagc catcaagcta
     6001 tagaggatat atctctaatg agaggtatac caaatatggt tgtattaaat ccagcagatg
     6061 ctttagaagc aagacaagct attcttacat ctattgatta taatggacct gtttatataa
     6121 gattaggtag agctgcaact cctgatgtaa acagtgaaaa ttataaattt gaaataggaa
     6181 aaggaactgt tttaagagaa ggttctgaca taacagtaat agctacagga ataatggtag
     6241 caaaagcctt agaagcagca gaagaacttg ctaaagaagg tataaatgta gaagttgtta
     6301 atatatctac aataaaacca ttagatgaag cattaataaa agaaactgct aagaaaactg
     6361 gaaaagtagt tacagcagag gagcacagta taataggtgg tttaggttca gctgtatgtg
     6421 aagctttagc agaaactaaa gatgtagtag ttagaagaat tggtgttaaa gatgtatttg
     6481 gtcaatcagg tacaccagct gatttattaa aacactatgg tttaactaca gaagatatag
     6541 taaaaaatat aaaagagttg ttatagatat aaatatatct aaataacaaa ttgatttgac
     6601 ataagtcaat taaagctgtc ttaaaaatag aaagaaattt ctacaatggg acagctttat
     6661 ttttacaata ctttattttt atgataatta taaaatagtc tgaaagtaat caaaaaaatt
     6721 ataaaaagag gtgctattta atgaaaatat ctattggatg tgaccatggt ggttatgaat
     6781 taaaggaata tataaaggaa aatttaataa ataaaggtat agaagttgaa gatgtgggta
     6841 caaattcaaa agaaagtgta gattttccaa tatatgcaaa aaaggtaagt caaaatgttc
     6901 aaagtaaaaa atctgattta ggcatacttt gttgtggaac aggaataggt atgtctattg
     6961 cagcaaataa gtttaaagga atcagagcag ctgttgtttc tgactgtttt tcagctaagg
     7021 caactagaga acacaataat acaaatgtac tttgtttagg agaaagagtt attgggaaag
     7081 gattagcaat gagaattgtt gaagaatggt taaattcaag ttataatgga gaaagacatg
     7141 caagaagatt acaaatgatt gatgatatag aaaaagaaaa tttataaata tataatttat
     7201 aacaaggggg agcgttatgt ctattatttg tgcttcaata atgtgtgcag accaaatgaa
     7261 gttaaaagag gaattagaag ctcttgaaga agcaggtgta aaattacttc attgtgatgt
     7321 tatggatgga atatttgtta aaaacttggc tatgggacca gagttattaa aatcaataag
     7381 tgaaaataca acaatacctt tagacataca cttagccaca gaaactccag ataaatatat
     7441 tgatatgatg tcttatataa agccaaaata tatttctttt catgtagaat caagtacaaa
     7501 tgtaaaagct gatatacaaa agttaagaaa ttatggtata gggccagtgt tagcaataag
     7561 ccctcaaaca agtgtggata aaatagaaga atatataagt ttagttgagg gtatcttagt
     7621 aatgacagta aatccagggt ttgcaggtca aaagtttaat ctttctgtat tggataagtt
     7681 ggataaactt acagagatac taaaagatta tgataatcct ccatttatag aggttgatgg
     7741 aaatataaat aaggatacta ttaaacttat gaatggtaaa aaagtagata tatatgttgt
     7801 tggtacttca gctttattta atgataagcc tccaatttca tataaagata aaatagaaga
     7861 actaaaagaa agtataaagt aaccatgtaa taaaattata atatgtttct gtttaatagt
     7921 aaaaatttag gtggaaacaa gaaattttat aatatttaat aaaaattttg gttgagtaag
     7981 gagaattttt tatgggagac ctatataaac tacaaaatgg tactgatatt agaggaatag
     8041 cgtatgaaaa tgattctaaa gaagttaacc taactgtaga agaagtaaaa aaaatagcta
     8101 aagcgtttca tatttggtta aaagaaaaga ctaagaaaga taaagtaact gttgccatag
     8161 gaactgattc taggataaca ggaagccaat ttagaagtac tgttattgaa actttaacta
     8221 atgacgattg taatgtaatt gattgtgaga ttgccacaac tccagctatg tttatgacaa
     8281 ctattatgga tggatacaat tctgatgggg cgattatgat tacagcaagt cacttacctt
     8341 attactataa tggtttaaag tttttcacag aaagtggagg tttagaaaaa actgatataa
     8401 aagagatgtt ggatatagct gttaaaaatg atagtgtaga ctatgaggaa gtaaataaaa
     8461 aaggtgaagt tatagtaaaa aatttaatag aagattattc taatttattg atagataaaa
     8521 ttagaaaagg tgtaaattca agtaaaaatt atgaaaagcc tttttctggg cttaagatat
     8581 tggttgatgc aggaaatggg gctggtggat tctttgctga aaaagtttta cacatactgg
     8641 gagcagatac gacaggtagt cagtttttga acccagatgg tatgttccca aatcatatac
     8701 ctaatcccga aaacaaagaa gcaatggaaa gcatatgtaa agctgtgtta gataataaga
     8761 gcgacctagg aataatcttt gatacagatg tagacagagc tgctattgta ggtaaaaatg
     8821 gcaaaccaat aaataaaaat gctcttatag ctgttatatc atcaatagta ttagaagaac
     8881 atccaaaaac agctatagta acagattcga ttacttctga aggacttgct aaatttataa
     8941 atgaattaca aggaagacat catagattta aaagaggata taaaaatgta ataaatgaag
     9001 ctataagatt gaatagtgaa ggtgaagagt gccatttagc tatagaaacg tctggacatg
     9061 ctgctttaaa agaaaattat ttcttagatg atggggcata tttaatagct aaaattttaa
     9121 taaaagtagc aaagctatcc ttggaaggaa agactataga agaattgata gaaaaccttg
     9181 aagaagctca agaagagaaa gaagttagaa taggcattaa taaagaagat tttagaccat
     9241 atgcagagga tatattaaaa gatttagatt ctcatgtcaa gagtattgat ggatggtctg
     9301 tagcaccaaa taattttgaa ggcataagag ttaattgtga tgaatcaaat ggagatggat
     9361 ggtttttact tagaatatca ctacatgaac cattattagc tttaaatatt gaatcaaata
     9421 aaattggcgg agcagaacaa atttattcta agttaaaact tttcttagaa aaatatgatt
     9481 taaaaggtct ttagggtaaa tagaaagaat atatttatat tgaagaataa atgtaaattt
     9541 gtacctaaga ttacaaatat tacaaaattg taatcttagg taacagagat gtaactgatt
     9601 atggaaacac tatgtgatat tatagataca gagagatgaa atatgtg
//
LOCUS       DAELTA010000141         9615 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.141, whole genome shotgun sequence.
ACCESSION   DAELTA010000141 DAELTA010000000
VERSION     DAELTA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.141"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            900..2258
                     /locus_tag="KPG13_002892"
     CDS             900..2258
                     /locus_tag="KPG13_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG0986592.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2365..3159
                     /locus_tag="KPG13_002893"
     CDS             2365..3159
                     /locus_tag="KPG13_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG0986593.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3152..5458
                     /locus_tag="KPG13_002894"
     CDS             3152..5458
                     /locus_tag="KPG13_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0986594.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5597..6874
                     /locus_tag="KPG13_002895"
     CDS             5597..6874
                     /locus_tag="KPG13_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0986595.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7057..7779
                     /gene="yqeC"
                     /locus_tag="KPG13_002896"
     CDS             7057..7779
                     /gene="yqeC"
                     /locus_tag="KPG13_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG0986596.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7886..8455
                     /gene="mocA"
                     /locus_tag="KPG13_002897"
     CDS             7886..8455
                     /gene="mocA"
                     /locus_tag="KPG13_002897"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG0986597.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8452..9471
                     /locus_tag="KPG13_002898"
     CDS             8452..9471
                     /locus_tag="KPG13_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG0986598.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3804 a         1003 c         1797 g         3011 t
ORIGIN      
        1 caactgagat acatagctta ttaagttttt ctgtcttatg agaattatat tataaaatat
       61 tcataaatat tttgagttta aattaaataa ttattttatg cttagattaa ttgatattta
      121 attaatttaa taacaaaaac agtgaaagag taaataatat tatttacagg gggtaaagca
      181 aagtatgtta aataaattgt acttttggcc ccgtacatag agaaatctat gtaggacaca
      241 actttaaaac cagtaaatcc taaagccttg caactacaat aaaagataga aagaaacttt
      301 tatgaatgtg gtgaaaacca gaaacaatag tgaggatggt atatggtgag agcctaagta
      361 cttaaaaatg gacatttggt cgccaagcct agaaatagga aggtcaaacg actatctcga
      421 gagagagtac actataagcg attgatagtg gaaacgggtt gctcttagca ggtaatgctg
      481 aagatgaaga aatagtctac gctcatatga aagtatgagg tcactggatt tgaagtgaca
      541 tctataaagt agcgtttata ggtaaatgtt taaaacttga aacatgtaaa tacttatgaa
      601 aacttataac ttataatgat taagattgaa agatttaaat tgaattaaat ttactataaa
      661 aaattaaaaa ttcatagcta taatactctt atatttgtaa agcatggtat ataatgtata
      721 caaaattaaa gaatacgtgt atttgttaat aattaaattt attgcatctg ttttttaaga
      781 ctaaaaactt aaatatatgt tggagtatag ttgatattta tgaacatcat taatttaata
      841 tattagattt ttaagtattt atataattca ataagtttta tgtaacggga gtttttatta
      901 tgaaagcaaa aagtacatca aaatttgaat tagacggaat accaccacta aaggaagcaa
      961 tacctttagg gttacaacac ttaatggcaa tggtaatagg tagttgttta ccagcgatat
     1021 tgattgcaaa tgcagctgga cttaatcata agatgtcaac attattgatt caagccgcat
     1081 tagtttttgc aggattatca actttattac aattatatcc aatacctttg tttaaaggtt
     1141 ttaaattagg aggaggaata ccagttataa tgggagctac agctatgttt ataggagctg
     1201 gggtatctgt tgcaagtaag tatggactac cagtattatt tggctctcaa ataatagctg
     1261 gtatagtaat aattataatt ggttttggtc ttaaaaaaat acgatttata tttactccag
     1321 ctgtatcagg aacaatagtt gcatgtatgg gtcttggact ttttccaata gctataaaaa
     1381 acttagctgg gggtatgggc aatgagactt ttgggaatcc aattaatttt atagttggaa
     1441 tatctgtatc tcttatgata ttaggattaa ataagtatgg aaaaggatta tttaaagatg
     1501 catcaatatt agttagcata atttttggat acattttatc attaattttg ggtatagtaa
     1561 atttttcttc aattcaggag tttactttgg ttgcacttcc aaaaccactt gcttttgggc
     1621 tagatataag acttgaagtt gttgtaatgt tttcaataat atatttagtt gagatagctg
     1681 atataatggg tgcttgtaca ctttctgcgg taggagggct taatagacaa gtaacagatg
     1741 aagaactttc ttctgctgtt ttaggaaatg gagtactctc tacaataggt gcattatttt
     1801 ctgcaactcc aatggggatg tttggacaaa attctgcaat cgtttccaat acaaaggttg
     1861 taagtaaatt tgtactagct attggaggaa ttggattatt tttggcagga atatcgcctt
     1921 tattagctaa tttaataaga acaattcctc cctgtgtggt tggaggagca acactcgtta
     1981 tattttcaac tcttacaaca tctggtttaa gacttgttag tatggatgga tttaatcaag
     2041 aaaatagcat gatattaggt ctttctatgg cttctggtat tggattcatg gtagctcctc
     2101 aagttcttga aaaatttcca aaatttatag agacattact tgcagattca agtgtagttt
     2161 caggagcaat ggtagccata ataattcaag ctttatatat ggtaaaattt cctggaaata
     2221 aaagtgataa ggttgaggat aaaaataaaa gtttataatg taaaggttta atatatgtga
     2281 attaaatata ctacaattat aaatataaat aaaagattta ttaaaaactt ttgtgtttta
     2341 ataagttaat gatgggggga taagatggtt actataaagg aatattcagt tccacagagt
     2401 ttagaagaag catataaaat tttaatttct aaaaaaaata atgttatttt aggtggatgt
     2461 ggatttataa aattaagtaa taaaaatata ggaacagcga tagatttaaa agatataaat
     2521 ctaaattata taaaagagga taaaaaaaat attttgatag gtgcagatac ttcacttaga
     2581 agtttagaaa ttgataaaac tataaaaaat tactgtagtg gaatactttc aagcgctgtt
     2641 tctaatatag ttggtgttca atttagacaa ggggctagaa ttggtgcaag tgtattttca
     2701 aagtatggct tttctgactt aataccagca cttttggttg ttggagcgaa ggtgagatta
     2761 tacaataaag gaatactaga gttaagtgag tttttggata gtgaattaag tagggatata
     2821 ttggtcgaag taatactacc aaaaaaagat gcaatagctg tttttgactc aataagaaaa
     2881 tgcacaggag atttttctgt attaaattca gcaatgctaa aagaaaatga tacttacaaa
     2941 atagcaattg gggcaagacc tcaaaaagct agattagcac ttgaagcgag taatatatta
     3001 aataaagaaa aagatataga taaggcatct attgttgcta gtaaggaact tacttatgga
     3061 agtaatatga gagctagtaa agagtacaga aaggatatgg caagtgcatt agttaaaagg
     3121 atgtataatg ctattgagga gggaatgtat aatgattaat aaggggataa gaaagataga
     3181 tagtacagca attgtcacag gaaaacctct atatactgaa gacctcatta tgcataaaga
     3241 tgttttgaca ttaaaactac ttagaagtcc acatgctcat gctaagataa agaatataga
     3301 tacatcaaaa gccgaaaaaa ttccaggggt tgtgggtatt tatacgtata aagatgtacc
     3361 acaggtcaga tattgtgttc caggtcaaac agaaccagaa gcatcacctt atgatagagt
     3421 tttacttgaa gatacagtta gatttattgg agatgaagta gctttagtgg ctgctgaaga
     3481 tgagaaaaca gcacttaaag ccttaaaaat gataaaggta gattatgagg ttttaaagcc
     3541 tgtgtttgat gcaagagaag cagaaggaaa tgaagttgtt atacatccag aaaaagacat
     3601 agtagagcca tacccttttg gattggatgc aaaaaataat atagtatcaa aagaattttt
     3661 tgaatttgga gatgttgaag ctaagcttaa agaaagtgat tttgtggttg aatcttcata
     3721 caaaacccaa agtcaatcgc attgcatgat ggaaactcat aggtcatatt catataaaga
     3781 tataaatgat agaattgttg tagtttcatc attacaatca ccacacaatg taaagagaat
     3841 acttagaaaa gtattgggta taccgtcttc aaaaataaga gtaattaaac ctagaatagg
     3901 tggtggattt ggaggaaaga atatagcagc aacagatata tttactgcat ttgttactat
     3961 gaaaactggt agaccagcaa aatttgtatt tacaagaagt gaaacttttg taggttctaa
     4021 tagtagacat gaaatgtttt ttgatgttaa agttggtgca gataaagaag gaaatttaaa
     4081 agcaatagag ctaaagtcat taaacaatac aggtgcttat ggtgggaatg gtgtctctgt
     4141 ttcttccgaa tctggacaca ataccttacc aatatataat gatattgatg ctgttagatt
     4201 taatgctaga acagtatata ctaataggtt accagcagga gcacttagag ggtatggtgc
     4261 tacacaggga atatttgcaa tagatagtgc tattgatgaa ttggcacata agattggaat
     4321 ggacccactt gaatttagaa ttaaaaatat aatagatagg tataccaatg gaaaagcagc
     4381 tgaagacgaa atattaagtt gtgagctgag acaatgtata gaaaagggaa agaaattaat
     4441 taattgggat gataaatatc catgtaaaga tataggaaac aacaaagtaa gagcagtagg
     4501 tatggcagta gcaaatcatg gttctggtat accgcgtgta gatatggcaa cagtcactat
     4561 aagaatggat gaagatggca catataaaat attatctggt cttgctgact taggtcaagg
     4621 ggctgataca attcaagctc aaattgctgc ggaagcttta aatactactg tagatagaat
     4681 ttctatttat acaggtgatt cagacttatg tccctatgat acaggagcat ttgcttcttg
     4741 tacgacaacg gttacaggaa atgcaacaat aaagacagca aataaattaa aagatatatt
     4801 actttcagtt gcagagtcaa agctaggatt taaaaaagaa gatttagagt tgggaaaaga
     4861 cagggttttt gttaaaaacg ataatagcaa gtttgttcta ttagaggaat tgggtagaga
     4921 atctgttgga ggaataggat ttgctagtga tgatgtacca gtagttttgg aagcaacaga
     4981 atcttttggc atgttgaaaa aaataaaacc atttatagca ggatttgcag agatagaatt
     5041 agataaaaat acaggtgaat ataaagtcat aaattttgca tgtgtacctg attgtggaac
     5101 agttataaat ccaactttag ctagaataca agtagaaggt ggtgtgatga tgggtatagg
     5161 gtttgcaatg tatgaagacc ctatatttga taaagatggt agattattaa cagatagttt
     5221 cctacagtat aaagttccta caaaaaaaga agttggaaat atattggtag attttgctga
     5281 gaatttcgac ccaggaggtc cttatggtgc taaatcatta ggagaaatag ttatacatac
     5341 accagcacca gctatagcaa atgcaatatt taatgctaca ggagttcgtt taagagagct
     5401 tcctatgact tttgaaaaag tttatatggg tatgaaaaat aataataaac aattataata
     5461 aataataaga tgcataaaag caagtaaatt taaattattt tgtaaaataa agtaattaaa
     5521 tggacattat atatggtgtt ttgtaaaaat accgtatata atgtctagta gagtatattc
     5581 taaaggaggt gagttcatgt caaacagaat aaaaaatata cactatggaa ctcagagaca
     5641 acaattaaaa aacttaacta aaaaagaata tcaagctcta cgagagatgt gcttcttagc
     5701 taagaactta tataatgttg gtacatataa tgtaagacag cattatttca atcaaggaga
     5761 acatcttaac tatgaagcta attatcatgt atgcaaagaa aatgagaact ataagcttct
     5821 caatagtaat atagcgcaac aaatattaaa agaagtagac agttcgttta ggtcattttt
     5881 tggattaatt aaattagcca aagaaggaaa atatgacttt agagggataa aatcacctaa
     5941 atatttagat aaagaaggat tttttagcat aataataggt caaataagaa tcaaagaaga
     6001 tgggatttta aatgtaccta tgtctccagc atttaaaaaa ctatatggga aagtaagtat
     6061 aaaagttcca caaaatatac tagagaaaaa aattaaagaa ataagaataa tacctaaaca
     6121 taaagctagg ttctttgaaa ttcagtattg ttatgaaata ccaaaatcag aggaagtttc
     6181 aaatcaaaaa aatgcactag ctatagactt gggtttagaa aatttatgta catgtgttac
     6241 caatttaggt gatagtttta tcatagatgg aaaaaggcta aaatctgtga atcagtgggc
     6301 aaacaaacag aatgataaaa tagaagcctt aaaattaaaa aagaatataa ctgcaataac
     6361 aagaaaacaa tttaaagttt ggaataagag aaataatcaa gttaaagact atgtaaataa
     6421 aacctgtttt tatataattg attattgtgt aaaaaataat attggaaatt taattgtagg
     6481 atataatgct aagttagaaa aaagtgataa tatggagaga aaaatatata gaaattttat
     6541 aagaatccca tttggagaga taaaaggaaa attagaatat ctgtgtaaaa taaaaaatat
     6601 caattttatt aggcaagaag aaagctatac tagtaaatca gatttttttg ctgatgattt
     6661 tctacctgat atcaatgtgg acaatgttaa aaaatatgac tttaaagggg aaagaataac
     6721 acgtggacag tacaaatcca gtgttggaat cattataaat gcagatgtaa atgcgagttt
     6781 taatatacta aaaaaatcac aagtagttaa tttatctaat ttacagaata atcctcaaat
     6841 attaaagcaa ccacagagag taagaatatc ttaattatat ttaaaagata aactgatagc
     6901 gtaagcttac agcttagcgg cgaattggat ccgccttgtt gtttacaaca gaatctattt
     6961 actttcgagt ttatggaatt tcaaaaatca cttttactaa aaaggcctaa ctataataat
     7021 taagagatta gaaaactggt tataagaggt gaaagtgtgg aactatcaaa tataatagaa
     7081 aagaatgaaa taataactgt agttggagca ggaggaaaaa cttcgtttat aaactatttt
     7141 gcaaattttt atagaaataa attgaaagtt cttcttacaa caacaactaa aatatatgta
     7201 ccaaacgatt atgacaatat aattattaca atagatggaa ctgttattcc atctatttgt
     7261 catggaataa cagtctgtgg cagttatata aataatgaaa ataaattagt aagtattgat
     7321 tcctctattt tggatgaaat tgtagaccaa tttgatttag ttttaataga gggagatggc
     7381 tcaaagagaa aaaaacttaa aggttggaat gctaaagaac cagtagtata tcatagaact
     7441 acaaaaacca tagggatatt agatataaca tcatttggaa tgaatattaa tgaagagaat
     7501 atacatagag tagaaatatt taaaaaaata gcaaatttag atacaagtag tattaatagt
     7561 agttctacag taagtattga aaacttaaaa aatatagtat taaatccaaa tggattattt
     7621 aaaaattatt ctggaaaaag agtattattt attaataaag ttgaaaatga aaaatataaa
     7681 aatttagcta taaaattaat agaaaatatt aaagaatatg aaagtgaaat agagatattt
     7741 tatggaagtg taaaacaaaa attttgtgta agatattgaa gtattaaaat agtttatatg
     7801 atattatata tatagataat atcaatagag atattataaa aatcatagaa ctatcaatat
     7861 attataaata ccaaggatga tatatatgat aaatgcagtg ataatggcat caggctttgc
     7921 cagaagaatg ggagcaaata aacttttatt aaattataaa ggctgttcta taatagaaca
     7981 tgtatttaga gaagttagca agattgattt tcatcaagtt gtagtagtaa gtcaatatga
     8041 agaggttttg ttattgtgta ataaatatgg ttttgaatat atagataata aaaatgcaaa
     8101 tataggtcaa agtgaatcta taaaattagg agttttaaat agctcaaaat gtgatggtta
     8161 tatgttcttt gtaggagacc agccatttat tgataactta tatatagata aaattataaa
     8221 tacttttaaa ttagatagag attttatagt aattccaaga tacaaagata tgtgtggaaa
     8281 tccagtcata ttcccatata gcaagaaaga agaattgttg gcacttaaag aagatgaaaa
     8341 aggtaaaacc attttaaaaa attcatcaaa aataaagtat gtagaagttc ctaaagaaat
     8401 gttatttgat atcgatacaa aaatggacta tgagagaatt gggggtaaat tatgaagcta
     8461 ataaaaacta tagatgctgt tggacaagtt ttatgtcacg acataactca aataatacct
     8521 ggagaattta aaggaagaaa atttaaaaag ggtcacatag taaaagaaga agatattcca
     8581 gtgttattat ctcttggtaa agataatttg tatgtatggg aaaagtcaga aggaatggtt
     8641 catgaaaatg aaggcgcatt gtttttaaaa gaactaactg ctggagaaaa tttagatttt
     8701 agtgaaataa aagaagggaa aatagatttt atagcagcat gtgatggtct attaaagata
     8761 gatgttgatg cactttttga tttgaattgt gttggagaaa taatgatggc tactcttcat
     8821 aataatttta tagtaaataa aggtttaaaa gtagctggaa caagagtgat acctttagtt
     8881 atagatgaga aaaaattaga agatgcaaaa aaagtagttg gtaatagaaa aatagtaaat
     8941 gtagtacctt ttaaacctaa gaaagtagga attgttacta caggaaatga agtgttttat
     9001 agcagaattg ttgataaatt tggaccagta atagaagaaa aagtaaaagg ctttggttgt
     9061 gaagttatag gacagactat atgcccagat gataaagaaa ttataaagag tgcaataaaa
     9121 gaatttataa gtcaaggagc agaacttatc tgttgtactg gtgggatgag tgtagaccca
     9181 gatgatgtta cgccaacagc tataaaagaa actggagctg atttagtgac atatggttca
     9241 ccaatattac caggagctat gtttttactt gcttattacg gagaagttcc aattatgggt
     9301 ataccaggat gcgcaatgta tcacaaaact acagtatttg atatagtttt atctagggtt
     9361 ttaattgatg aaaagcttga taagtatgat atagctagat atggtcatgg tggcctttgt
     9421 atgaactgtg atgtatgtac ttatcctgca tgtaattttg ctaaaatata aagttattaa
     9481 aaaagagtat ttacaaatta agtactcttt ttaaatatga aacaaaacag ctagaatgat
     9541 gttttaaaaa aagcatattt atttagaaat tataataact tattttcaat gtgtaaaaga
     9601 atgaataaag ttgca
//
LOCUS       DAELTA010000142         9532 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.142, whole genome shotgun sequence.
ACCESSION   DAELTA010000142 DAELTA010000000
VERSION     DAELTA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.142"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            335..913
                     /locus_tag="KPG13_002899"
     CDS             335..913
                     /locus_tag="KPG13_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0986599.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="KPG13_002900"
     CDS             952..1530
                     /locus_tag="KPG13_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0986600.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="KPG13_002901"
     CDS             1616..2245
                     /locus_tag="KPG13_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0986601.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="KPG13_002902"
     CDS             2235..4994
                     /locus_tag="KPG13_002902"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG0986602.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="KPG13_002903"
     CDS             5081..6643
                     /locus_tag="KPG13_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG0986603.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="KPG13_002904"
     CDS             6644..7780
                     /locus_tag="KPG13_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG0986604.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="KPG13_002905"
     CDS             7844..8920
                     /locus_tag="KPG13_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG0986605.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..>9532
                     /locus_tag="KPG13_002906"
     CDS             8898..>9532
                     /locus_tag="KPG13_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HBG0986606.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGN"
BASE COUNT         3750 a          996 c         1833 g         2953 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tt
//
LOCUS       DAELTA010000143         9381 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.143, whole genome shotgun sequence.
ACCESSION   DAELTA010000143 DAELTA010000000
VERSION     DAELTA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.143"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            615..1028
                     /locus_tag="KPG13_002907"
     CDS             615..1028
                     /locus_tag="KPG13_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986607.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            1096..1512
                     /locus_tag="KPG13_002908"
     CDS             1096..1512
                     /locus_tag="KPG13_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986608.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            1513..1725
                     /locus_tag="KPG13_002909"
     CDS             1513..1725
                     /locus_tag="KPG13_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986609.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            2122..2292
                     /locus_tag="KPG13_002910"
     CDS             2122..2292
                     /locus_tag="KPG13_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986610.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            2835..3713
                     /locus_tag="KPG13_002911"
     CDS             2835..3713
                     /locus_tag="KPG13_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG0986611.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            3749..5644
                     /locus_tag="KPG13_002912"
     CDS             3749..5644
                     /locus_tag="KPG13_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0986612.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            5912..7819
                     /locus_tag="KPG13_002913"
     CDS             5912..7819
                     /locus_tag="KPG13_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0986613.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            7809..8258
                     /locus_tag="KPG13_002914"
     CDS             7809..8258
                     /locus_tag="KPG13_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0986614.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            8358..9245
                     /locus_tag="KPG13_002915"
     CDS             8358..9245
                     /locus_tag="KPG13_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0986615.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
BASE COUNT         3777 a          933 c         1580 g         3091 t
ORIGIN      
        1 tagatctaat aactcctata ataaaattta tcattagtta catatagaat aaacctcagt
       61 tttgattact gtaggaacta ggattatatg aagctaccac tcatagattt cagttttttc
      121 tgttgtatca tagcatagaa tagtaaattt atctgaaact ttattttaaa tttagaattt
      181 gcatatagat aaattatggg cattattctg atgattcgtc tccaataatt aaaaaaagaa
      241 tgataaaata cttgaaaata ctagtcttta ttgactagtg tttttttatt tcagtgataa
      301 aatataccaa ttatacttca gttacaggaa atgatgtttt gagggtacat tgggaggcat
      361 ttaggttgta taattataag tttcaatgtt atatgctcat tagaataata gttagtaaat
      421 gatatgattg agatgataga tgaaataaac taatttagta ctgtattgta atgtttatta
      481 taaaatcttt tataaaatat atttatgaag atattattga atattttcaa tattctattg
      541 caatttattt ggaaaagtta tatatttata acataaggtg ttataaatat ataactttta
      601 aggggtgatt ttatatgtct aaatcagagc ttggtgttaa aacaaatctt gtttcagagt
      661 tacttatgat atcctatttt tttatagact cattattaaa aaagtttatt tttaaagagg
      721 gaaatctttg tattactttt ataattttat tcgctttttg ggcatgtgac ttcatgtata
      781 ataaaaaata taatgattta gttgacgagg cgtcaaaact tatgttatta aaggttgatc
      841 atattgcatc aaaaataatg ttatattcta ttttgctaat agctgttttc ttttcagcaa
      901 attatacaag aggtttggat atatcaaatt tggatatagg tgtaattctc ttagctattt
      961 tatttataca atctttatct aaattaattt tgtatataca ttttgataga aagggaatat
     1021 gcaactaagt gataatattg ataaaaacga aaattaaaga gtatagagaa tataattttt
     1081 aaggagtgat tttatatgtc taaatcagaa actggtgtaa gatcaaagct tatcctggag
     1141 ttgcttgtaa taacttattt ttttatagct gtattagcca ctaagtatat ttttaaaggt
     1201 ccaagcactg cttctcttcc agggttgagt tggttaatag tttttggatg ttattcaatt
     1261 tataataaaa aatataatga tttagttgat gaatcatctg aatttatact atcaaaggtt
     1321 aataagattg caataaaaat aatgatatat tctatagtaa taatatctat tttctttgca
     1381 acaccttata caagtaatat aaatatatca aacctagata caggtataat cctcattgca
     1441 gttttattta tacagtcttt atctaaatta gtgttgtata tatactttga taaaaggggg
     1501 atatgtaact aagtggcaat attgatgaaa acaaaaatta aagaatacag agaaaaactc
     1561 cttatgacgc aaaatgaact tgctaaattg gttggggtaa gacgtgagac tattgtacac
     1621 ttagaaaatg ggaaatataa tccttctttg aagttagcta tggatatcgc taaagtattc
     1681 gatactacag tagaaaattt gtttgaattt attgaagaag aatagtggaa agtttataaa
     1741 catttataag atggataata aaatatagtt atttaattat taagtttgtt aaaaatattt
     1801 atataatata atatatgatt aaggtcagat tataattgtt ttaagataaa atgtaataaa
     1861 atttaatgga gaaattttat aggtagataa attttaatat ttataagatt atgttaagga
     1921 aacacttaaa aattagatta aaattgtggg tatttaaatc aactaaaaga tattgactat
     1981 ttagtcaata tcttttttta ttaaaaagga atatacatat aaataggtta gattaaaaat
     2041 tgggataaat atgatttata tagcatcatt taattaaaag gagctatatt agtattcatt
     2101 catacaattg gataatatac aatgtcagcc ttgctttatg gtgtttgtaa taaatctatt
     2161 tcagtttcta ttgtaattaa tatgcaagaa tttaagatag gagtatctat ggcttatggt
     2221 gtattagtaa ttggtatatg ctatatagca ttaggaataa tttttaaatt agatagaaaa
     2281 agatttacat aagataacaa aggctaattt ataaattggt gcttagaaaa ccaaatcact
     2341 ttattagcta gagatgaatt tggagggtgt cactttggtg ttttatgtaa agtttatgga
     2401 agtaaaacat tagataatcg tgagtaagat ttctataaat cgtatatact attggaatcc
     2461 aagaaaaggt attggccaaa tagctaatat ctttttaatt tttttgactc taatatagca
     2521 ttaactattg catgagtatc tttaaattta tagttaatgt atcttaattg tttattataa
     2581 aatctaggaa ggctaaagca taaaacactt aaaaagagaa gatgtacatg agaaatataa
     2641 ttaactatga tgcagacttc taaaaattat ttagtgaaaa ggaacttaga gtgtataata
     2701 aaagttcctt ttcactaagg aaaacgattg ttattttctg acattcatta ccctgatatt
     2761 atttaaatat aaacaacaac aaaattaaca agaagcaaat tcaacctaaa atttaaaata
     2821 tcgggaggta caatatgtta ataaacatga aggaaatgct aaaagtggca caagaaaatc
     2881 aatttgctgt accagcattt aatataggaa gtgggcaaat acttaaagca gtagtacaga
     2941 gtgctaacga aaaaaatgca ccagtaatac ttgcaataca cccaaatgag ctaagttttc
     3001 taggagatag ctttgtagct agttgcatag aagaagcaaa taagagtaaa gtacctatgg
     3061 taatacactt agatcatggg gaaaataaag aacaaatatt aagggcaata agatgtggat
     3121 ttacatcagt aatgattgat ggctcacatt taccatatga agagaatgta gctatatcta
     3181 gggaaatagt agaaatagca aaaggattaa atgtgtccgt agaaggagaa cttggaacaa
     3241 taggaacaac aggaacatca agtgaaggtg gtactgatga aataatatat acagattcaa
     3301 aattagctaa ggattttgtt gaaaaaacag gagtagacac actagctatt gcaattggta
     3361 cagcacatgg tatatatcca aaaggtttta aaccagagct taaactagat ttattaaaag
     3421 aaataagaga agtagtagat atacctttag ttcttcatgg tggttcatca aatccagatg
     3481 aagaaatagc acaagcagtt aaactaggag tttgtaaaat aaatatttcg agtgatgtta
     3541 agtcggcata ttataagaag tgcagagaac tattagaaca aaacccaagc ttatatgagc
     3601 cagatactat atatccacca tgtataaaag ctgcaagaga agttatagag tttaaaatga
     3661 atctattcaa tgctatagat aagttaaagt gcttttataa aaataaaaat taattatgag
     3721 gtaacaaatt aaaaggaggg gagaatttat gataggtaaa atactacgaa gcgattgtat
     3781 tgtactagat aaacaattac aaacaaaaaa agatgtgatt aattatatgg caaatcattt
     3841 gtaccatgga aattatataa ataataaaga aaagtttata gctgatatta ataaacgtga
     3901 aaaagaatca tcaactaata tgggtaattt aatagctata cctcatgcta agtcaaatgt
     3961 agtaaatcaa cctgtaatga tgtatttaag aacagataga gagattacag ataatgatgg
     4021 tcaaaaactt aggtcatttt ttatgatagc agttccagaa tcaggttcag atgaacatct
     4081 taagattata tctaaactag ctacatatct aatggaagaa aattttctta agcatctact
     4141 aagtgcagaa actccatacg aaatattaag tatatttaat aataaagctg cagaatcagg
     4201 agattttagt aataatcaaa ataaaggtct tatagttggg ataacaggat gtccaacagg
     4261 tatagctcat acttttatgg caaaaaaagc attagaggaa gcagggcaag aacttggata
     4321 tgaagttaaa atagaaactc atggagctat aggtgtagaa aataagttga ctaaagaaga
     4381 tataaaaaga gctgaatacg taataatagc ttgtgataaa gaagttcaac tgagtagatt
     4441 tataggaaag aaagtgctac aaaagaaagt agtagatgct aagaatgttt cattagctaa
     4501 aaatttaatt ataaacgctg aaaatggaat ggctaaagaa attaaaccag gtggaaagac
     4561 tttagaagat tcaaaagaaa tgcttcaagg gtcagagaaa aagggtgttt ataagcactt
     4621 aatgagtggt gtatctgaaa tgattccatt tgtagtgata ggtggaattg gtatagccat
     4681 agcttttatg tttggtatat ttgcatcaga cccaactcat gaaacataca accctatagc
     4741 tggattcttt ggacaacttg gaggcgattc agcatttaag ttatttatac cgatacttgc
     4801 tggttttata gctaaatcaa tagcaggaag acaaggattt gctcctgcta tgatagctgg
     4861 ttttatggca gttattggag gttcaggttt tataggtggt atggtagctg gttttgcaac
     4921 aggatatata gctttaggag taatgaagat aacagaaaat ttaccagatg tattaaaagg
     4981 agtaaatgct gtattgatat gtccactttt gattgcattt acgagtggag ctataatgtt
     5041 ctttgtagtt aatacgccag catcatggtt aaatgaagct ttacaatctt ggttacaaag
     5101 tatgtctgga actaataaag tattattagg tgcaatatta gcagggatga tggcatcaga
     5161 tatgggagga cctattaata aaacagcttc agcttttgga ttggctatgt tttcaagtaa
     5221 tatatttgaa ccatcagcag cattaatggt aggaggaatg gtacctccat tagcaatatc
     5281 attagctatg ttaatattca aaaataagtt tactaaagaa gaaagaaagt catctctacc
     5341 aactgctata atgggggcat ctttcataac agaaggcgca ataccgtttg cagctgcaga
     5401 cccattaaga atgattccag caaatataat tggttcttgt ataggtgggg caatatgtat
     5461 gagtttagat ataactttaa tggcaccaca tgggggaata tttgtaatac catttgcatg
     5521 tagtgcacca gttgcatata tagcttgtat attgattggt acagtaataa cagcattgat
     5581 tataggggta acaaagccta ctataaggag tgaaaatagt gaagttgaat tacttggtgc
     5641 ttaaattata aataatagta aaaataatta atggaataac aacaagtata tagaataata
     5701 ataaggatat ttagaatcag ttaactattt ctaaatatcc ttaattttta ttttgaaatt
     5761 atattatcta ttatatcaat ttagtaaggt gtatcataaa ttcttttatg atacagaagt
     5821 atcttagtaa ttttacatag ttagtaaata tgttaaaata gtacttaata taaacataga
     5881 aatatataaa cataggtagg gagggctaag tatgcttgtg tcgaggcaaa ttgaatttct
     5941 taaattgata ctaaacgaaa ataaatatag acctataaaa tactttaaag ataaattaaa
     6001 tgtatctgat aaaactttac aaaaagattt gaagatgatt gagaaatatt tagaaacttt
     6061 taatataaaa attgatataa aaagaggcta tggaatatta atagaagatt tagcaaagaa
     6121 gaacatagac ttaattaata gtttaaatat acaatcgaag gaaaataaat ctttatcagt
     6181 agagcagaga agaatggaga ttttaaagta tttattgcta aattcaaata atataacttc
     6241 aataaatcag ttagcagata aatattatgt aagtaaaact tctatagtaa atgattttaa
     6301 atatatagag gcatggataa aagaatacaa tttaaaactt aataaaactt tagaaggaac
     6361 taagattata ggtaaagaag tggatataag gaaatcgatt gccaaaatga tggatgattt
     6421 attagatgaa aattatgaac aacatgatat atctgagtta acaagattag attctgctac
     6481 atttagtgca ttgataaatt tatttgatat tgattctatt atatttgtag aaactataat
     6541 tacagaatta gaatacaatt taggatatac tataaatcag ccctactata taaatttaat
     6601 tacccatata cttatatgtt taaagagaat cgaagaaggt aatcaaatag aatctcagga
     6661 agaaagagaa atttctgtag ataatttgga cgagttagta tataaaaatg tcactacact
     6721 tatagataag atagaaaata gatacagagt aaaaataacg aaagatgaaa ttatgtacat
     6781 atatattttt ttggtatcat ctggatttag tagcgaaaat aatagtagtt acaatgatga
     6841 atcagataga ttaagtgaaa gtgaaaaagt ttcatctata atgatagaga atatgtcacg
     6901 atttttaaat ataaatctca aaaatgatga cttactacag aaaagtctag catctcatgt
     6961 aaggcctatg ttaaacagat taagatatga tatccaaata aaaaacccgt tgttaggaga
     7021 gatacaagag cgattttcag atgtgctggg attatgtatg atgactataa atattatgac
     7081 agagtatgat aatctgaaga atattagtat agatgaagtt tcgtatcttg ccacatattt
     7141 tcaagcggca atagaaagaa atatgagtag taaaagagta atagtagttt gtcatacagg
     7201 atatggaaca tctcagctcc ttgcagccag attaaaaaga gaatttcctg aatggactat
     7261 agttgatata gttcctatgc atcagttgag taagcgaaat ttagatgatg tagactttgt
     7321 aatatctact gtaaacttag atttgaaaga taaattacat atagttgtat cagtactatt
     7381 aatggaaagt gatattaata atataaaaaa tgctttactt tctaaaccta aaaaaagtat
     7441 gaacttagat acaaacttat taggaatgta tttagaagat aatatatatt ttaattttga
     7501 tgtagctcag atggaaagtt taataggtgt gaatttaggg agtaaatatg aatctatttc
     7561 attaggaaaa gatttaaata tatatataca taagcaaagt aaattaaata aagggataat
     7621 gaatatcaat aatgttacta gaacgattga tatttattta gaaattagta atcaaagttt
     7681 tttaaagaat attttaatgg aaatatcaaa tttatatagg caaaagaact atataaacta
     7741 tataatagat tgcaagagca aagaggatat taaaatatta tttaagaata atctattgga
     7801 gaaaacaaat ggaaattaga gaaatattaa cagaagaaag cattatttta gatttgaaat
     7861 caactacaaa agaaggcata atacaggaat tagtagataa attatacgat acaggaagta
     7921 taactttaaa aagtaagttt ataaaggcat taaaacatag agaaaaaata ggctctacta
     7981 gtataggtta tcaaatggcc atgccacatg gaaaaacaaa atatgtaaag aaggcatcca
     8041 tagtaatagg taaatctaaa aaaggtgtag attggccaag tatagatggc ttacctgtaa
     8101 aggtaataat tatgtttgct atgccacaat ctggaggtaa tgaagatatg atattaatat
     8161 ctaaaatagc ttacatttta ggtggtaaaa atataggtga aagactttta aatgcacata
     8221 caaaaaaaga aatattagat atattttatg aagtgtaggt attgtataaa ttataaattt
     8281 gaggttttta aaataaaatg tgtattcttt taaatagtat agattaattt ttagacacta
     8341 gagaggagag gatatatatg gcaatatatt tagaaatgcc taaattaata catgaatttc
     8401 cttttcgtac tctatcaaat gaaggagaag tattaacaac accacactgg cataaggaaa
     8461 tagaaatatt acttataaca gaaggtgtag taaatctatt tataaatgac aagcctatgc
     8521 aattaaaaaa aggcgagata gtgataataa aaggaggaga tattcattat atactgccat
     8581 caccgggaag tgaaagatta gtatttcaat ttgatatatc tttttttaat gatttaattt
     8641 tattaaatga ggagaaagaa agcttaatta atttattcag tagtataaaa aaatgtagta
     8701 gagattggtc agcaaaaata agaaagtcag ttttcgatat attgatagat atctacaatg
     8761 aagacctata caaaatagaa ggatatagtt atgcaataaa aggtaaaatg tataatttaa
     8821 ttcctatatt atatagacag attcctagag aaattgaaga aaatacagta gaatatgaaa
     8881 taaactctaa agagatatta gagagattga acaatatatt caaatatgta gaaaaaaatt
     8941 ataaacaacc tataaaatta gaagatgtgt cttatgaagt aggatttagt gtatattatt
     9001 ttactaaatt tttcaaaaaa aatacaggaa aaacatttat aactttttta aatgaatata
     9061 gattagaaaa agcaaagtgg atattactta ataacaatat atccattgta gatttggttg
     9121 aagaagttgg ttttggaagt acaaaaactt tttacagagt atttaaagaa aaaattggag
     9181 tctctccatt agaatttaaa aaaagatata atagcttgga gaaaaaaggg agtgtctcaa
     9241 aatgaactta gttcatgagg cactttcttt tatatgaaat caaatatatt ttcagtattt
     9301 caacaatgaa aattccattc atttataaag tctaaatttt agttctcaat acaacctcta
     9361 tttcgaaata aatcctttta t
//
LOCUS       DAELTA010000144         9370 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.144, whole genome shotgun sequence.
ACCESSION   DAELTA010000144 DAELTA010000000
VERSION     DAELTA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.144"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            156..1376
                     /locus_tag="KPG13_002916"
     CDS             156..1376
                     /locus_tag="KPG13_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0986616.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1413..2762
                     /locus_tag="KPG13_002917"
     CDS             1413..2762
                     /locus_tag="KPG13_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG0986617.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            2876..3438
                     /locus_tag="KPG13_002918"
                     /pseudo
     CDS             2876..3438
                     /locus_tag="KPG13_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3546..5879)
                     /locus_tag="KPG13_002919"
     CDS             complement(3546..5879)
                     /locus_tag="KPG13_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0986618.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(5876..6559)
                     /locus_tag="KPG13_002920"
     CDS             complement(5876..6559)
                     /locus_tag="KPG13_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986619.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(6630..7871)
                     /locus_tag="KPG13_002921"
     CDS             complement(6630..7871)
                     /locus_tag="KPG13_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986620.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(7882..8568)
                     /locus_tag="KPG13_002922"
     CDS             complement(7882..8568)
                     /locus_tag="KPG13_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986621.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            complement(8806..9150)
                     /locus_tag="KPG13_002923"
     CDS             complement(8806..9150)
                     /locus_tag="KPG13_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986622.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
BASE COUNT         3283 a         1423 c         1166 g         3498 t
ORIGIN      
        1 tgaaactatg caatcatttt ttatactata catatagata aatgattata tttattcaat
       61 attcttataa aaaacaaagt tctccatcga aaattatggc ataataattg cttattatat
      121 taataaaaca ataaaaagca attggaggaa taaaaatgca acaggctttg aaattaaaat
      181 atcaaaccaa agaattagga aaagattttg taatagaaag ttgtaattct ataaaaccat
      241 ggcttataaa cataagaaga gaattacata aaattccaga attagctctt gaagaaaatc
      301 ttactaaaca gaaggtaatt tcttatttaa aagaaatcgg aattgattat atggaattta
      361 caaaacacaa tggaattatg gcatatattt taaaagaaag tgcagataaa acgatttgta
      421 taagagcaga tatggatgct cttcctattg aagaagaaaa caacatacct tataaatcaa
      481 tacattctgg aaaaatgcat gcttgtggcc atgatgcaca tacaaccatg ctacttggag
      541 catgtaaagt tttacattca ataaaagata aacttaatgt aaatgtaaaa ttcttatttc
      601 aaccagcaga agaaggtttt ggaggagcaa aattcttagt tgaagatgga tgtttagaaa
      661 atcctaaggt cgattatata ttcggacttc atgttatgcc acatatcgaa acaggattta
      721 tcgaaataaa atatgataca ttaaatgcta gtgtagacac tataaaaata agtatcaaag
      781 gtaaaagagc tcatggtgca tatcctgaaa atggtattga tgcaatagtt acagcttcac
      841 aaattgttac atcattacaa actataataa gtagaaactt agaaccaaat aatgctgctg
      901 ttttgactat aggtaaaata tatggtggtg atgcacacaa tgtaatatgt gaagatgtaa
      961 aactagaagg cactttaaga accttaaata gcaaaacaag aaactttatg attgataaaa
     1021 tagctaaaat tgttggagac actgcatctg catttggttg tgttgggact ttacatgtaa
     1081 gtgatgaaaa ctatcctgct gttataaatg aaaaagaatt agtagataca gttatatcaa
     1141 atactaaaga attacttgga gaggaaaaat ttatattgag accaaacccg tctttaggtg
     1201 gtgaagactt ttctttttac acagaacatt gtaaaggagc attcttccat ttaggatgta
     1261 aaaatgagga aaagggctta atttcacctc ttcatacctc tagctttaat atagatgaag
     1321 attgtttacc aataggtgtt atgatgcatg taatgaatac tttgtatttt aattagtaca
     1381 gtaattaata aaagaatagg agataaatta atatggaaaa agctaagaaa ttaggtctat
     1441 ttagtatgat attattaggg attaatagca taataggttc aggtatattc ttactaccag
     1501 gtaaagttta taatctagca ggtcaaaaca gtatgtttat atacattttt gctacactct
     1561 tagttctatc aatactttta tgttttgcag aagttggaag catgtttgat aagaatggag
     1621 gagcatatct atactccaaa aaagcttttg gagattttat aggatttgaa gttggtacaa
     1681 tgtcttgggt tattagaatt atatcatggt ctactctagc agtaggattt gcaacagctt
     1741 taggttcatt ttggccagaa tctgccactg aatacaaagg ttatatagct gcaatactcg
     1801 taacactact ttcaataaat agcttatttg gaattaaaag cacaaagata atgaataatg
     1861 taataactat agctaaatta gtaccattaa tagtttttat aatagttgga atattcttta
     1921 taaaatttgt aaatattgtt ccttcaggaa atgtagttaa ctctagtatg ggtccagcca
     1981 ttatattagt attttacgca tttaccggct ttgaatcatt tattgtggca agtggagaaa
     2041 tggaaaatcc taaaaaaaat cttccagtcg cattaataac cacaatattt atatgtgcaa
     2101 taatttatat attaattcaa attgtctgta tgggtatact tggagataga ctatttgaaa
     2161 acagtattcc aattgcagat gcttcaagtg tatttttagg caattatggt aaatttttca
     2221 tatctgtagc aacattaata tctatatttg gtataaatat aggttcatca atagtaactc
     2281 ctaaatgtgg ttcttctcta gcagaagaag gttctcttcc tgcatttata ggaaaaacaa
     2341 ataaatatgg tgctccatat gtagcaataa taatatcttt aatttgttgt ataccacttg
     2401 tgctaactgg aagttttgaa caacttgcag taatgagtgt aattgcaaga tttgctcagt
     2461 acataccaac ttgtttatca gtaatagttt taagaaaaag aacagatgtt aaggcctctt
     2521 ttaagattcc atttggtcct gttataccaa tggtagctat tttaggtagc ttatggttgt
     2581 tacaacaagc ttgggcagaa gatatttcaa aacctataac tcaaaataga gttttaatag
     2641 gattaggggc aatgttgtta atagctcctc tatatatatt tatgaaaaaa aataaggaaa
     2701 tcgagcataa aaataaatca gaagattcta aattatatat ttcgagagaa aaaattcaat
     2761 aatattttac aaactattta aaaattattt ttctaatact atagaaataa ttagaaatgg
     2821 agatgttacg tatctaaaag tagttgataa cacggatatt ggtactttta tagatatggg
     2881 acttacaaaa gacatactag ttccttttaa agctaagact tatccattgt ttagagatga
     2941 aaaatattta ttctatatat acttggataa aagcggaaga atagctgcta ctacagacat
     3001 agattcatat ttagaaacag accatactta caatgtagga gatgttgtaa atggagttgt
     3061 atatggattc caaaccaaca actctgctat gatatgtgtt gacaataaat atgctggtgt
     3121 aatccttcat aatgaatact ttacagaatt aaaagctggg gatgttcttg aaaatttaca
     3181 tgttataaaa atatacgatg atggtaaatt aggactttct cctagaggaa acagaaaaga
     3241 tgaacttgat accttagaaa ataagatttt aagctattta gaaggttcag atggatatat
     3301 gagattcaat gataaatctg atcctaaaga tatttctatc ttatttaatt caagtaagaa
     3361 aattttaaac gtgctcttgg agtacttatg aagaaagact taatttatca agatgaagat
     3421 ggaacttatc taaagtaaat tatataacac aaaaatcgaa gtaaattatg aaagatattt
     3481 tctatttcat atttacttcg attttttatt taaattacta cctctattta aattactact
     3541 tatatttatt gtgcatttac tacctctatt atgtctaatt cacaaatctt tttaattggt
     3601 tttcttactg caagcaaagt tattattatc atagttacta caatgataaa cagtaaatca
     3661 aaaggtattt gccagtctat atggaatttt aatgttacta aggatacaaa tatatatcta
     3721 tgtaatatta atccaagtac actccccaat aaacagccac ttacagcata tgtacttgat
     3781 tctactatta ccatttttct taattgctta ttggacatgc ctattgcacg cataatacca
     3841 tagtaattta ttctacttgt aacgcttata ttcatactgt ttatgatatt aaacatactt
     3901 ataatcgcaa ttataaatac aaacccatat acaaaaactg ccatagtgta aaatgaattt
     3961 ttagcctcta agttaccttg tcgcttatct cgaacctcta aattatcatc tagaacattt
     4021 cttatatttt ggattaaatc ttcaggagca tcttcatcaa cttgagtatc aattattata
     4081 tagtctttta tatctgttat actagtaaaa gtattttcag atgttataat atttccaata
     4141 tatttgtctt taacatcaaa tgaaattgaa gaaagtatac ctgctacctc tatctctttc
     4201 ttagaatttc catttttaat atatatctta tctccaatat taaacccatg accttcttct
     4261 accaatccga tattttctga ctctaattta tcttcattga tttccccttt tactagatac
     4321 tttttggccc agtcaaattg atttttttca taagaaatta gctgagatgt tgcctcctta
     4381 ttattgtatg tagcctctac atccgtcatc attctactgt atactttttt tattcctttg
     4441 atattactta tttcattaat atcgtcttta gtaagaacag acttaaagtc agcgtcaaca
     4501 atagatatat gagcagcttc tggttttata ggtttaagtg cctgatacat aaagtctata
     4561 aatacactaa aacctaaaaa caatacaata ctaatagcaa atgaagaagt cataagtata
     4621 aaagattttt ttcttgaaaa ggcatgattt attcccatag aaacatcaat tttagaactt
     4681 cctgcgttag caattttact tgcttttcca ctagttaaaa tttctttgaa cattagatta
     4741 ccacgtatag cacttagtgg agacacccca gaagcttttt ttgctggaac aatagctgat
     4801 aatattactg ttaaaaatcc tactattatt ccaaacacaa taccaataaa gctaaccttt
     4861 aaaattggca tcgaaccaaa taactctgta ttcacatatt tgagaaatat agaggatata
     4921 atggttacaa tacatcctaa taacaatcct agtggaatag cctttacact tagaattaaa
     4981 ctttctaaaa caacaaattt tttgacttgg agttttgatg ctcccaagca acgtaatagt
     5041 ccaaaaaact ttattctctc tattacaccc atattaaagc tattatatat catgataata
     5101 cttgctatta acacaagtat aaaaagtcct cctgctgtta agtaaagtgg tttagaataa
     5161 tcagagtcac cttgacctat aatagaaagt aatttttcat ttttaccgat ttgtttatca
     5221 cttaaattga aggtattctt aatatcagaa gtagcttttc taatattaac cttgtcttta
     5281 aatgtaatat aataataatc atatgtttct gattttttga tagcttgtgt acctaatatt
     5341 gaaagcatta atccatgctt atctgaggct cttaatcttg ataatgatga atattcacct
     5401 actatcttat aatttttatt tctaccatct gctaatttta ttttgataaa atctcctact
     5461 tttagattaa agcttttaac tgcatctaca tctattaaag tctcattctc cacaacagga
     5521 taacgacctt ttattatttc tagacctaat tgttttgcta aatcttcatc taaaccttgt
     5581 atcaaaatct cctttgattt aaactctcca ttatcaatga cttgtcctct accatagtga
     5641 gctatatcta ctctactact tatttctgat atagcttctt cattaagatt tttaagtact
     5701 atgtgccaat ttccatcatc ttttataaat tgtacttttt gacattgtat agccatgtct
     5761 gctagtccaa aaatagcagt aactaaacat actgcaatga caatacataa aattgtagtt
     5821 ctattttttt tcttatgtac ctttgcatat tgtggtacta gttctaaata gcttctcatt
     5881 ctctattcac ccctaggtct ttaagcattc catcagaaac ctgtagtatc ctatctgctg
     5941 tagaagctat atttgtatta tgagttatca taataatagt ttggttatat ttttctgctg
     6001 tcacttttaa aagtgaaatt acatcactgc tatttttact gtcaagattt cctgttggct
     6061 catctgccaa tataattgat ggctttgtaa tcaatgctct tccaattgca accctttgtt
     6121 gctgaccacc agataattga tttggaagat gactttttct atctttaaga cctaaaattg
     6181 tcaatacttc atctagataa atagtgtctg gttttttgta atctagtagt aaaggaaata
     6241 ttatattttg ttccacagtt aattcgggta taagattata tgattgaaag ataaaaccta
     6301 tattacgacg tcgaaaaata gtctgttttt cttctttcat tgaacatatt tctttaccat
     6361 caacaaatat ttttcctgag gttggagtat caagacctcc taaaagattt aataatgtac
     6421 tcttacctga gccagattct ccaactacag taacaaactc tccctttgaa actgtaaatg
     6481 atacatcttt taatgcctct acttttgtat atccatttcc atatgtttta cataaattat
     6541 taaccacaag aatatccatt tgctctatct cctttctgat gattaatata agaataattc
     6601 atacacctta ctgtaagatg tatcctatct tacatattag taagctttaa aaaacttaga
     6661 gtaaatgtac ttccttgtaa aaaattactt tctacagtta tggttccatt gtgctgttct
     6721 atgatagatt taactagtgg caaccctaat ccaaggcctt gattactttt tgaaaactta
     6781 ctcctataaa atcttttaaa tatatgatgt atatcttcaa tatgaattcc atttccggta
     6841 tcactcactg aaataatagt tgttataggt gtttcaaccc attctatcat tattttatcg
     6901 ttctcttttg tattatctaa tgcatttttt ataaggttgc ttaaagcttc tgatatccat
     6961 tcataatcgc aaaacaagtt tgtattttct agacctctta tagagatact ctttccttct
     7021 ttttctgctc tagtttcaaa actatataaa attttagtaa tcaacttttt tacgttaaca
     7081 ctagctttgt taagaattat tgtctttgaa tctatctttg taatctttaa caagttttgt
     7141 atcaatgatt ctatacgctc taattcattt tgactcttct gcacaaaatt ctcaacaatt
     7201 tcactattat gactctcttc ctgcaatatt tgattataca tcatcaatgc tgtaagtgga
     7261 gtttttagtt gatgtgatat atctgaaatt gtatttttaa gaaattcttt attctgcttt
     7321 tctttcaata tatgtgtgtt tagagatgtt gacatagcat ctattgaacc aaataacttt
     7381 gataaactac cttcttcata agtatttaac cgtacattta tatttccatt cataaatgaa
     7441 tcaattttat aactagcttc ttctatcttg ttctgttgcc taaaaaaata cacatacaat
     7501 ataaaaataa tcatagtaaa gaacacaaat aaatacaaaa acatagtttt tatatattta
     7561 ccttgaaaca atttcacact tggcataaaa ctatcatctg taccaaagtc atatctagct
     7621 gtatttaaga tttcttttcc ttttaaagta tatttatctt cattagtagt aaatactgct
     7681 ggtatttttg atttatcaac acccatacta tttaagtatc cagctatatt ataatcatgt
     7741 tctaacagtg cacttttata atcattagtt atatttataa ctactatttg acctactact
     7801 ataaacatca ataaaacgga tataatcacc aaaaaaaata tttttatgtc tttattagaa
     7861 aataccttca tattaacacc ctcactttat aacattccat ttgtaaccca ttcttctaac
     7921 tgttaataga ttttttggat tttcaggatt atcttcaatc ttatttctta accttcttac
     7981 atatactgac agagtattat catctacaaa actaccattt ccatcccata acttatccaa
     8041 tatatttttt cttgtaagta caatattttt atttttcata agtaagcata ataatttata
     8101 ttctgctgta gtaagttcaa gctcaatttc atttttaaaa acacgatttt caagtaattt
     8161 aacagtaata ttatttgatt ttagttcagt tacattttca cttgtaaaat tataacgtct
     8221 aagtaaagct tttattcttg aaattaactc atttaattta aaaggttttg ttatgtaatc
     8281 atctccaccc atatcaagtc ccatcactac atttacttct tcatcagaag cagttaaaaa
     8341 tatgattggt acattagaag cttctcttac ccttttacaa atatcaaaac catcaccatc
     8401 aggtaaggat acatccaaaa gcagtaaatc ataatttttt tctttataaa tttgcaatgc
     8461 ttcttctact gttcttgcta catcaacact aaatccattc ttttgtatag aatattctaa
     8521 tccatatatc aaacttaaat catcttcaac caacaatata ctactcatct caatcacttc
     8581 cattttttta ttttttactt tatatatatt atatacctaa cttattatat ttttactctt
     8641 ttaaaaatac aataccttga aatcaatgat ttaaatattt taaaattatt tttatatgat
     8701 tttatatagt taatttaata ctttgaaatc ttaataataa aaaaagctta aaatacacta
     8761 agtattctaa gctttattca atcacatttt atatatttat attatttatt tttttaaaat
     8821 actattttca atattgcctc tattaaacat ttcattatca ttatctaaag agatatcact
     8881 tatatttcta acagttgttg aatagttacc actaagttct atctcactga aatgcatatg
     8941 cactatcttt tgtattacaa atcccattga tgcaagtaaa atagcaaata ttatatactg
     9001 actaaagtta ctcatataat aatttataat ggtatattca tttcctttga ctgttaaatg
     9061 ttttgtagat agcatagtat atatatattc agtacaattt ataaatacat atataccata
     9121 aatagctaac actacagata aacaatacat tgtgattgaa atctttgata tttttttaaa
     9181 cttactatct ttacaacact taatatttgt tatttatcat aaacaaggtt attgtattat
     9241 caataatttt taggttacac ttattattag tcttgtgtta aaacgacagt agacttaaga
     9301 gtagagtttt attaagccaa tatttatatt tgctatacat tgtaccccta ttacatcata
     9361 catataactt
//
LOCUS       DAELTA010000145         9249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.145, whole genome shotgun sequence.
ACCESSION   DAELTA010000145 DAELTA010000000
VERSION     DAELTA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.145"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..352
                     /locus_tag="KPG13_002924"
     CDS             <1..352
                     /locus_tag="KPG13_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0986623.1"
                     /translation="IIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEIDLDM
                     VNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNESLIL
                     NNLKNLEKQYNSSY"
     gene            365..877
                     /locus_tag="KPG13_002925"
     CDS             365..877
                     /locus_tag="KPG13_002925"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG0986624.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            951..3158
                     /locus_tag="KPG13_002926"
     CDS             951..3158
                     /locus_tag="KPG13_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG0986625.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3183..3632
                     /locus_tag="KPG13_002927"
     CDS             3183..3632
                     /locus_tag="KPG13_002927"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG0986626.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3647..4261
                     /locus_tag="KPG13_002928"
     CDS             3647..4261
                     /locus_tag="KPG13_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986627.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4352..5908
                     /locus_tag="KPG13_002929"
     CDS             4352..5908
                     /locus_tag="KPG13_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG0986628.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5898..7160
                     /locus_tag="KPG13_002930"
     CDS             5898..7160
                     /locus_tag="KPG13_002930"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG0986629.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7234..9021
                     /gene="aspS"
                     /locus_tag="KPG13_002931"
     CDS             7234..9021
                     /gene="aspS"
                     /locus_tag="KPG13_002931"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG0986630.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         3735 a          992 c         1770 g         2752 t
ORIGIN      
        1 aattataaaa tattataata aaacagacct attatattta aagaatgtag ttagtaatat
       61 agttccatca agagatgaat ttataacaat ttataaacaa gcactcataa aaaaagaaat
      121 tgatttggat atggtaaata taagggaaac ttttaatgta attccattaa agttttttac
      181 gatattaaat gtatttagag aattaaattt acttgacttt aatttaaatt atgaaaaaaa
      241 ttctgtactc ataagaatat tgccaaaacc acagaaaaaa ttagacttaa atgagagttt
      301 aatactcaat aatctaaaaa atttagaaaa acaatacaat agcagttatt aggaggcgtt
      361 tcccatggat ttaaaaaact ttataaggaa tattgatgat ttcccaaagc caggcataga
      421 ttttaaagat gtgacaactc tatttaaaga tggagatgca tttaaatatg cagtggattc
      481 tatagttgaa gaattgaaag ataaagatgt agatttagta ataggaccag aagcaagagg
      541 atttttgatg ggtacacctg tagcatatgc tttaggagta ggatttgttc caattagaaa
      601 acctggaaag ttaccaggtg aagttgagag ttatgagtat ggattagagt atggtacaga
      661 tactttagag atacataagg atgcaattaa aaagggtcaa aaagtagcaa tagtagatga
      721 tttattggct actggtggaa ctatggaagc cgctgccaag cttgtagaaa aattaggtgg
      781 agaagtagtt tctatgcaat ttttgattga gctaaaattc ttaaatggga gagaaaagtt
      841 gtctaactat gatgttaatt ctcttataaa atactagtac ttttataata gttggtataa
      901 caccaactat tttcatatat attaaaattt tatacaaaat aggtggttat atgcatgata
      961 aagaattaca agaaataatt gaaaaaataa aagtttatgc acctaatgca aattttgaaa
     1021 tggttgaaaa agcctataat cttgcaaaaa aagcgcatga aggacaatat agaaaatctg
     1081 gggaaccata ctttattcat ccattagcag ttgcaaatat tttaatagag atggaattag
     1141 atattgaaac tataacagca ggtttacttc atgatgttgt agaagataca gaatatacct
     1201 atgaagacat aaaaaaagaa tttggcaaag aagtagcgga tttggttgat ggagtaacta
     1261 aacttggtca aataaaatat cgttcgaaag aagaaactca atctgaaaat ttaagaaaaa
     1321 tgtttttggc aatggcgaag gatataaggg ttatacttat aaaacttgct gatagattgc
     1381 acaacatgag aacattaaaa tacatgcctc cagaaaaagc aaagtataaa gcaaaagaga
     1441 cacttgaaat ttatggtggt atagctcata gattaggaat ttccaagata aagtgggaac
     1501 tagaagacag agcattaaga tttatggacc cagaagggta ttatgattta gttagtagag
     1561 tatcaatgaa gagaagtcaa agagaggatt atattcaagg aattgttgaa cttcttaaag
     1621 ataagtttaa agatttaagt ataccatgtg atgtatatgg tagaccaaag catttttaca
     1681 gtatatatag aaagatgcaa aaaaaacata agacttttga agagattttt gatttaacag
     1741 cagtaaggat attggttgat aatgtaaagg attgctatgc agtacttggt atagttcata
     1801 cattatggag acctattcca ggaagattta aagattatat agcaatgcca aaacccaata
     1861 tgtatcaatc attacacacc actgttgtag gtcctgatgg agaaccttta gagatacaaa
     1921 taagaactca tgagatgcat aatatagctg aaaatggtat agcagcccat tggaaatata
     1981 aagaaggtat ctctagcaaa gaatcaaagg ttgaagaaaa acttcaatgg ttgagacaga
     2041 tgatggagtg ggaaaaagat ttaaaagacc cacaagagtt tatggatgca cttaaagaag
     2101 atgtatttaa tagtcaagtt tatgtattta cgccaaaagg agacgtaatt gaattaccag
     2161 cagaatcaac acctatagat tttgcctata gggttcatac aaatgtaggc aataaatgtg
     2221 taggagctaa aattgatgga agaatagttc ctattgatta taagcttcaa aatggtaata
     2281 ttgtcgaggt acttacatca tctaattcta atggaccaag tagggattgg cttaatatag
     2341 tgaagacccc aaatgcaaaa agtagaatta gacaattttt caagaaggaa agaagagaag
     2401 aaaatgttga ccgtggaaat acaatattag aaagagaatt taagaagcat gatataccac
     2461 aaaaagaccc tcttgttgaa aaatatatgg cattagttgc taagaaattt aatcagccaa
     2521 gtgttgagga cttaattgcc actgtaggtt atggtggtat aatgtcatct caagtggtat
     2581 ctaaggtaaa agacctatat ttaaaagagg ttaaaaaagc taaaaaggaa caacagatac
     2641 aagaagaaga tttaaataaa cacaatataa gtgaaacaga atacagtaaa aagagaaaga
     2701 aaaacgcatc acaaggaatt atagtaaaag ggcttgacaa tatcttagta agatttgcta
     2761 agtgttgtaa tcctcttcca ggagatgaaa ttgtaggata tattactaag ggtaggggag
     2821 tagccataca cagagctgat tgcccaaatg caaacaataa tggtgatttc ttccaaaata
     2881 gattggtgga ggtttcatgg agttcagcag gaaaaggtaa gtttgaagcg gaaattcaaa
     2941 taaaagctat tgatagaaga ggaattgtta atgaaataac tcatattgtt gcaatggata
     3001 aacttagttt aaacggaata aatgcaagaa aaggtaagga tagtattgta agtataaact
     3061 tacttgtaga ggttaatgac acagaggaat taaaaattct aatgaaaaaa ttaaaagcaa
     3121 ttcctggtgt agaaagtatt tatagaatga caaattagag ttaaaataat agggaggaaa
     3181 ctatgagagc agtagtgcaa agagtatctt catctaaagt tacagtagat gaaaacacta
     3241 taggtcaaat aaataagggg ttactagtat tgttaggagt gactcatgat gataaatcaa
     3301 gtgatgtaga ttatatgatt gataaaatat tgaatctgag aatttttgaa gatgaaaatg
     3361 acaagatgaa tttatctcta atggatatag gtggagagct tttagtagta tctcaattta
     3421 ctttatatgg agattgcaga aaaggaagaa gacctggatt tagtaatgca gctaggccag
     3481 aacttgcaaa taacctttat gaagaatttg taaaaaaagc aaaagataaa ggtgtgacag
     3541 ttggtacagg tcaatttgca gctcatatga tggtagagtt aactaatgat ggtccagtaa
     3601 caatactatt agactcaagt aaatcttttt aagggggaaa actttaatga ttataaaaaa
     3661 atttgtagat agttattttg gagtaaatac ttatgtatta ggtgatgaaa agactaaaaa
     3721 atgtgcagtt atagacccag gtggaagttt agtagagaca ttaagttttg taaaagaaaa
     3781 tgaattaaat attgaatata ttatccttac tcatgggcat ggagaccata taggatatgt
     3841 taaggatatt aaagaaaaaa ctggtgctaa agtagttgca catgttgatg aaaaagaatt
     3901 attaaatgac aaaaataaaa atctaagtta tacaatgaga tgtggagcgc aagagtttga
     3961 tgcagatatc tatgtaaatg ataaagacaa gcttgaactt ggtgaattaa aaatgacttt
     4021 cttgcatact ccaggacata ctcaaggatg tatgtgtata agagttgaag atgaaatgtt
     4081 cacaggagat actttgtttg ctggaagtat tggtagaact gatttatata gtggagactt
     4141 taaccaaatg gaaaaatcac ttaagaaatt atgtaaatat gaagatgctg ttagagtata
     4201 tcctggacat ggaccaacca gtactctagg ggttgaaaag aagacaaatc cttacatgta
     4261 attttatatt agtatttaaa aaagtataaa atatttaata actatattag tgagggggtt
     4321 cttctactgt taaatagaag gagttaagtt gatgttgtat gtttttttaa aaggacatga
     4381 ttataagtat gaagtagcag aacttataaa attgtttact agtgaattta agtttaaaga
     4441 ctctaatgct aacaataata tagagtcaaa gcatttaata aatagattga tttatgagaa
     4501 tggtgtatta ttctcaagta ctgaatacta tgaaaatgga aacttaaagt acgagtcaat
     4561 acagaatata gaaaatatga atcttgaatt tttagatgga tttgatagca gcaaaacgat
     4621 tgaagaattt cttaatagcc tagatagtga aagtaagagg aactttaaaa agctgtgtaa
     4681 agaaaatatc aaaaaaagca tgtttttagt gcttaaacaa gtatttaatt cttatgtacc
     4741 atggggaatt ttgactggta ttagaccagt caaaattgtc cacaatttaa tggataaaaa
     4801 gctagatgat aatagcataa gaaccattct aaaagataat tattttataa tagatgagaa
     4861 aatagattta gctcttgaaa tagctaaaag agagcgatta tttatgtatc ctatagataa
     4921 aaataaaatt tctctttatg taagtattcc attttgtcca actagatgtg tatattgttc
     4981 atttccttca aattcattaa aacaatttgg acatttaaaa agagaatatg tttataaatt
     5041 gattgaagaa attaaggggt ttgccaaagt tattaaggat actaagaaag aagttgaaac
     5101 tctatatatt ggtggtggaa ctccaactac acttgatgag gaagaattgg atttgcttat
     5161 aaattcattg ttcaatgaat tagatttaag taaaattaaa gaatttactg tagaagcagg
     5221 tagacctgac actattacag aacaaaaact aagagtgtta aaaaaacaca atgttagtag
     5281 aattagtata aatcctcaaa ctatgaatga tgataccttg gtaaagattg gaagagagca
     5341 tagagtatct gatttagtag attgctttaa tatggctcgt agaatgggat ttgataatat
     5401 aaatatggat ataattttag gtcttataga tgaagactta aatatggtaa agaacacatt
     5461 ggaggagata aaaaaacttt ctcctgaaag ccttacagtt catacattag ccataaagag
     5521 agcatctaac ttaaatataa atatggatag gtataaagaa catctaacac aatatgaaga
     5581 aatggtaaaa atgatagatt tatcaatgaa atatgcaaaa gatatgggat taaatccata
     5641 ttatatgtac agacaaaaac atatgttggg aaatttagaa aatataggtt atgctaaaga
     5701 aggatatgag tgcatttata atatacagat tatggaagaa aaacaaagta actatgcatt
     5761 aggtgcaggt gctatatcga aatttgtcta tgtagatgaa gatagaattg aaagagtaga
     5821 aaatgtaaaa aatgttgaac aatacattga aagagtagat gaaatgataa aaaggaagaa
     5881 agaagaggta tataaaaatg ttgactaaag ctcctagagg aactaaagac ataactccaa
     5941 aagaggctta caaatggcgt tatgtagaaa ataaatttag agaaatatgt gctttatatg
     6001 gatatgaaga aatggtaaca cctatatttg aacatacaga acttttcaag agaagtgttg
     6061 gagatactac agatatagtt caaaaagaaa tgtattcttt taaagataaa ggtgatagag
     6121 aaattaccct aaagccagaa ggtacagcag gtgtagtaag agcatttata gaaaataaat
     6181 tatatgcaga tactcaacca acaaaattat tttatgtgac accttgtttt agatatgaaa
     6241 gaccacaagc tggtagacaa agacaatttc accaatttgg aatagaagca cttggaagtg
     6301 atactccttc aatggatgct gaaataatcg cactagctgt tcaatttttt aatgaagttg
     6361 gattaaatga tttggtagtg agtataaatt cagttggctg tccagtttgt agaaaagagt
     6421 acaatgcttt attaaaagag tatcttgact caaaagctga tatattgtgt gatacttgca
     6481 atgagagaag agaaaaaaat ccaatgaggg ttatagactg taaaaatcct acatgtaaag
     6541 aaaatatcaa agatatacca tttattgcag accatttgtg tgatgattgt aaatctcact
     6601 ttgataaact tcaagaatac ttaaaggaaa tgaatataaa ctttgtaatt gataaaacaa
     6661 tagttagagg cttagattac tatagaaaaa ctgcttttga aattatatct aatgatatag
     6721 gagctcaaag tactgtatgt ggtggaggaa gatatgatgg tcttgttgaa caattaggag
     6781 gaccaaaagg tataagtgga attgggtttg gactaggaat tgaaagactt ttacttacac
     6841 ttgaaggtaa tggaattgaa atagaaaacc ctcaatctac ggatatattt atagtaacta
     6901 taggtgaaga agcaaataca agaagcttta aattgttaaa agacttgaga caaaatcata
     6961 taagtgcaga taaagaccat atagagagaa gtgttaaagc acaatttaaa tattcagata
     7021 aaataaattc aaaatttaca atagtaattg gagatgatga acttaaaaat gacactgcga
     7081 cattaaaaaa tatgaaaaca tcagagcaaa ctactgtaaa attaagtaca ttagttgaag
     7141 aattaaagca aaagctgtaa aataagaaag tatgatgttt tgtgtattag ataaatatat
     7201 agcattaaaa ttaaataaaa tggaggtagt atcttggaaa ctttaaaggg tttaaagaga
     7261 actcactact gtggagagtt gagagaaaaa aacataaatg aagaagtcgt acttatggga
     7321 tgggtacaga aaaaaagaaa tttaggtggt cttgtatttg ttgatttaag agatacaagt
     7381 ggattatgtc aaatagtttt tgatacagat gtagataaag aagcttttga aaaagctgaa
     7441 aaattagggg ctgaatttgt aatagctgta aaaggaaaag tatgcgaaag acaatctaaa
     7501 aaccctaata tgccaactgg tgatatagaa atatttgcaa ctgaacttag acttttaaac
     7561 aagtcagaaa ctccgcctat ttatataaaa gatgatgatg atgtttctga agcactaaga
     7621 ttaaaatata gatacttaga tttaagaaaa ccatctatgc aaagaaattt aaaattaaga
     7681 cataaagtaa tgaatattac aagaaattac ttatcaaaca atagattttg cgaaatagaa
     7741 acaccatttt taatagctcc aacaccagaa ggagcaagag attatctagt accaagtaga
     7801 gttaatccag gaaaatttta cgcattacca caatcaccac aattatataa acaattattg
     7861 atggtaagtg gtatggatag atatttccaa atagttaaat gctttagaga tgaagactta
     7921 agagcagata gacaaccaga gtttacacaa atagactgcg aaatgtcttt tgttgagcaa
     7981 gaagatgtta tgagtatgat tgaaggtttg cttgaagcga tatttaaaga agttttagat
     8041 gtagaattgg cacttccact tcctaagatg acttatgcag aagctatgtc taaatatggt
     8101 tcagataaac cagatactag atttgggtat gagttaacag atatatctga tgttgtttgt
     8161 aactgtggat ttaaagtgtt tgctgatgct acacaaccag gaaaaagtgt tagaggtata
     8221 aacgtaaaag gcaaagctga cgactttact agaaaacaaa tatcatcact agaagaacat
     8281 gctaagacat atagagcaaa aggtcttgct tggatgaaag taggacaaga aggagtaact
     8341 tctccaatag caaaattctt caatgaagaa gaaatgaacg ctatacttac aagaatgaat
     8401 gctgaagtag gagatttact tttatttgta gctgataaaa actcaatagt atttgatgct
     8461 ttaggacaag ttagacttga agtagcaaat aggcttaatc tattggataa aaatgtatat
     8521 aacttactat gggtaactga gttcccagtt tttgaagaag atgaagaaac aggcacattc
     8581 tcagcaatgc atcacccatt tacatcacct atggatgaag atttagacaa gttagaagaa
     8641 ggtgataagt catctttaag agcgaaagct tatgatattg tattgaatgg ttatgaaata
     8701 ggtggaggaa gtgttagaat atcaaattct gatgttcaat caagaatgtt taaagcactt
     8761 ggttttacag aggaaagagc taatgaaaag tttggttatt tattagaagc atttaaatat
     8821 ggtactcctc ctcatgctgg tctagctttt ggtcttgata gacttgttat gttacttgca
     8881 ggaactgata atataagaga agttattgca ttccctaaaa atcaaaatgc tgtttgccct
     8941 atgacaaatg ctcctacatt ggcggaagat gagcaattag aagaattaag tataaaagtt
     9001 gatataaaag ataatgaata atatgtagtt gcaagaaact aaaatgtaaa aagtctggat
     9061 ataaaatata tttatattca gacttttttt attagaatta ttttatattt tttctctata
     9121 tattttcata aattatactt atattcttaa ccaatataat taaaatacta tgtttaaaaa
     9181 ttttacataa acttggttgt tttagaaact acttctgtaa cgagtacacg attttaatta
     9241 aagaatata
//
LOCUS       DAELTA010000146         9114 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.146, whole genome shotgun sequence.
ACCESSION   DAELTA010000146 DAELTA010000000
VERSION     DAELTA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.146"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..1551
                     /gene="hflX"
                     /locus_tag="KPG13_002932"
     CDS             274..1551
                     /gene="hflX"
                     /locus_tag="KPG13_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG0986631.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1784..2281
                     /locus_tag="KPG13_002933"
     CDS             1784..2281
                     /locus_tag="KPG13_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0986632.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2331..2855
                     /locus_tag="KPG13_002934"
     CDS             2331..2855
                     /locus_tag="KPG13_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0986633.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            3167..4531
                     /locus_tag="KPG13_002935"
     CDS             3167..4531
                     /locus_tag="KPG13_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG0986634.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            4670..5836
                     /locus_tag="KPG13_002936"
     CDS             4670..5836
                     /locus_tag="KPG13_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG0986635.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            5953..6735
                     /locus_tag="KPG13_002937"
     CDS             5953..6735
                     /locus_tag="KPG13_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG0986636.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(6854..7507)
                     /locus_tag="KPG13_002938"
     CDS             complement(6854..7507)
                     /locus_tag="KPG13_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0986637.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(7531..8895)
                     /locus_tag="KPG13_002939"
     CDS             complement(7531..8895)
                     /locus_tag="KPG13_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG0986638.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         3537 a         1026 c         1460 g         3091 t
ORIGIN      
        1 aatccttctt atgatataag tagcttgttt tgtattactt agatttgtaa ggagtttttt
       61 tattgaaaaa actgcataat atatttggga ggaggtgtaa gaaagtgatt ttttatataa
      121 gattgagaaa ataatgcacg attatggtta ttataatagt aaataagaag tgtcatttaa
      181 ggataagata aaaagcaaaa ttcttgatga atttaattat agagccataa tctcgtgcta
      241 tagattaaat aaaaaatagc agataaggag aatatggata tggcgaaaaa aggaataaca
      301 gtaggattaa acataaataa taaatctgag gactttaatg aactaatgat tgaacttgaa
      361 aacttatgtt cagcatgtga tattgatgtt gtagggagta taactcaaaa tgcaaagcag
      421 gttaatagag cattttacat tgggacaggt aaagttgagg aaatattaaa cttaataaag
      481 aaagaaaata tagagatagt tatattttac aatgaacttt ctacatcaca actaaaaaat
      541 cttgaagaaa agcttaattg tgaaattata gatagaacgg cattgatact tgatatattt
      601 gcacaaaggg caaaaactag agaagctaaa cttcaagtag aagtagcaag tttaaaatat
      661 atgttaccaa gacttatagg ttcaaatgaa aatttaggac gtcaaagtgg tggagttggt
      721 actaaaaata gaggttctgg tgagaaaaaa ttagaacttg accgtagaag aatagaagaa
      781 aagataacat ctttaaatag ggagttggat gatttaaaat tccaaagaga aactcaaaga
      841 agtatgagaa gaaaatcaaa tcttccaaat gtagctctag tcgggtatac aaatgctggt
      901 aaatcttcaa taatgaataa actagtagat atatttaaaa actctgaaga aaagaaagtg
      961 tttgagaaaa acatgttatt tgctacactt gaaacttctg tcagaaatat agttcttgca
     1021 aataacaaag aatttttatt atctgatact gttggttttg taagtaatct accacatgac
     1081 ttagtaaaag catttaggtc aacattagaa gaagcatgtg aggcagacgt actgttacat
     1141 gttattgata tttcaaatcc aagctataaa agtcatatta aagtgacaga agatacttta
     1201 aaacaaatag gtgcagatgg tatacctatg atacatgtgt ataataaaat tgatttaata
     1261 gatgtagaag tattggatag aattttagat agtatcgata aagaaggtat atttgtttca
     1321 gttaaaaaag atataaatat agataaaatg ataaaatgta tttgtgatag catatttaaa
     1381 gattatgtaa gatgtaagtt cttaattcca tatgataaag gtcatgtagt ttcttatttt
     1441 aacgaaaata cgagcattat aaatacagaa tacagagaag atggagctat tttagatgta
     1501 gaatgtagca atatagaata taataagtat aaaaagtatg ctttagagta aattttgaaa
     1561 aacttattat agcctaaaat atatttaatt tataatttat tataaattga ttgtaaatat
     1621 gattttatga aacggtatag ccataatgct atgccgtttt gccgtttata ataaaaataa
     1681 atttattgac ataatagtaa ctaattacta taatataaag taattagtta ctatttaaaa
     1741 ttatcaaaaa attaaaatct aagataaaga gaagtgtata ataatggatg cagaaagttt
     1801 aatgaaaaaa tatatggtag atgatgatag tagaaaacaa gctatatttg caagtatatt
     1861 tgtaatgcaa aataaacttc aaactacatg tgataaatta gacccagaat taacaatgaa
     1921 acaatggtta cttttagcta ttgcggactc tgtagatgaa actcttacat taaccaaact
     1981 tggagaatta atggggtgct caagacaaaa tgtaaaaaaa ctagctattg ctttagaaaa
     2041 gaaaggtttt atacaaattg agcagaatga aaaagattca agagctatct gtcttgtgac
     2101 aaaagataga gtgcaagagt attctaaaaa agtaagaggt atgcaggaaa aagtattaca
     2161 gcttttattt gaagatttta cagagaaaga agtggaacag ttattttttg gaattatgaa
     2221 attaagtata ggaattgaaa atgtgaaaaa ttatgtagaa aataatgacg ttggagaata
     2281 agcaagtatc aaatcaactt tgtatgacat cagagagggg gataatttat atgaaaaatg
     2341 tagtagtgat ttataagagt aagtatggtt caacaaaaaa atatgctaaa tggctttcag
     2401 aattattgtc ttgtgatatt tttgaaacta agaatattaa tatagaaaag gttatgaagt
     2461 atgatacgat tattttagga ggaggtcttt atgcttgtaa tatagcggga atttctttct
     2521 taaaaaaaaa ttataaagaa ttgcagaaaa aaaatttagt agtatttggc gtaggagctg
     2581 ctccatatga tgatgaagta ataaagaaat taagagaagt aaattttaag gatgaattaa
     2641 acaaaattcc ttgcttttat tgtcgtgggg catgggatga gacaaaaatg tcttttatag
     2701 attatactct ttgtagtttt tttaaaaaat ctatagctaa aaaagaacgt caaggtctta
     2761 aaccagcaga agcaccttta ttgtctggag aagcctttga ttggtgtaat aaagaaaatt
     2821 taactccaat acttgattat ataagagaac tttaagaggt atatatttgt aattatttat
     2881 tatctaaagt tagatttcat agaaacttac tagacattat ttataaaata atattattaa
     2941 agatggataa tttaattgtt tcaataaggt tataatttta aaaaatgttt attttaaaat
     3001 aaactatttt ataagcttac ttatacttaa taaggggctt tttttattgg aagatttatg
     3061 ttatattgag atgaatttat tatgatataa aaaataatgt taggataaat ttaaatcttt
     3121 ttctaaaaat gtattagtat aattcttaaa gaaaggattt ttaacaatgg atataaaaga
     3181 gttagttaaa atgcaaagaa aatattataa tacaggtaaa acaagagata tatcgtttcg
     3241 tatagaacag ttgaagaaac ttaagttagt tgtatcacaa aatgaagaaa aaatactttt
     3301 agcactaaag aaagatttaa ataaatcgga ttttgaaggt tttatgacag aggtaggcat
     3361 gttttatagt gaattaaact ttgctataaa gaatataaga aaatggagca aaataaaaag
     3421 agttaaatca agtatggtta attttccatc tatatccaaa gttgttccac aaccttatgg
     3481 tgttactctc ataatgtcac cttggaatta tccatttcaa ttagcactta taccattagt
     3541 ttggtcatta gcagcaggga attgtgttat attaaaacct tctgaatatt ctgtgtcaac
     3601 atctagtgta gttaaagaca tagttgaaga tactttttca aaagaatatg ttgcagttgt
     3661 tcaaggaagt caagaagaaa gtgaaaagtt actattggaa agatttgatt atatattttt
     3721 tacaggaagt actaatgttg gtaaaataat catgaaatca gcatcagagc atttaactcc
     3781 tattacttta gaacttggag gaaaaagtcc ttgtatcatt ttaaaagatg ctaatataga
     3841 ccttactgcc aaaagactta cttggggaaa actaataaat gcaggacaaa cctgcgtggc
     3901 tccagattat gttttagtgc atgaagatag aaaaaatgag ttaatagaga aaataaaata
     3961 ttatactaat aagtattttg gggataatgc atgtaataat gagcaatttc ctaaaatcat
     4021 aaatcagaga cattttaatc ggatattatc tcttattgat aaagataaga tagtctatgg
     4081 tggaaattat aataaggaga cattaaagat agagccaact atagtagaca atgttaattg
     4141 ggatgataat ataatgaagg aagaaatatt tgggcctata tttcccattt taacatataa
     4201 agatttagat gaagtaatac aaaaaattat acaaatgcca aatccattgg cactttatat
     4261 ttttacaaaa aataagtacc tagaaaataa acttttagaa atgattccag caggcggatg
     4321 ttgtatcaat gatacagtta ctcatatagc tacgaattac ttaccttttg gtggtattgg
     4381 tgaaagtggt atgggctcat atcatggaaa agctggattc gatacattta ctcactataa
     4441 gagtgtgtta aaaaaattaa atttggatgt ccctattaga tatgctccat acgataataa
     4501 attaattaag gttcttaaaa agataatgta ggttatattg tagaataata aatataaata
     4561 aaattgagta tttgtattct gaatatattt tatttttaat ttggtatatt tgttataaaa
     4621 gtggtgattt tgtgataaaa tttaattata tagaaataag gaggaaaaaa tgaaaattaa
     4681 ttggaataca aaatacacaa caatagcaat ttatacattt ataatagctg ctagtagtat
     4741 tatattttat ctagtatcat cacaaattga tgtattttct aataatttag atgctatatt
     4801 tacaacatta caacctttta taataggatt tgctattgca tatcttctta attttatttt
     4861 aaagttttat gaagacagaa tttttataaa aagtgagaaa ttaaaaaaac taaaacaaag
     4921 ttcaaaaaga ggcttgggtc ttttattaac atatgcaaca gctacattga tactttactt
     4981 atttatgcat ttcgtattgc cacaagtaat tgagagtatt gttggactag caaatgatat
     5041 acctatgtat gtaaacaatg caactaagct tattgataaa ttaatgactg atttaaatct
     5101 tgatgagcaa tactttaatt tggcagttga taagtggaat gaatttgtta catatattat
     5161 aaagtttgta acagatttaa tcccaatatt aggaagtatg ttaaagaatg tagcctctag
     5221 tatatggaat attgtactag gattaattgt ttcagtgtac ttattaattg ataaagaaaa
     5281 attctatgga ttaagcaaaa aaataactta tgcagtattt acagaaaaac aagctgccag
     5341 aattttagaa ttaactcata gaagtaacta tacatttgga agatttttag gtggcaaaat
     5401 attagattca ttcattatag gtattctaac atttgtaata cttacgttag ttaaaatgcc
     5461 atatacatta ttaatatcag ttataattgg tattacaaat attatccctt tctttggacc
     5521 tttatttgga gctataccat ctacgctaat agtgatgttt gtatctccta taaaagcttt
     5581 ctggttattg ttgattatat taataataca acagatagat ggaaatatta taggacctaa
     5641 aatacttgga gattcaattg gtatatctgc attttggatt ttgttctcct tattggttgc
     5701 aggaaaatta ttaggtttta ttggaatggt tataggagtt ccaatgtttg cagtaattta
     5761 ttctataatt aaggatattg ttgaaagtaa attggacaaa aagggattac cgacagatac
     5821 ttccgattat atgtagctaa aaaatattat gtataattaa aaatatataa attaagcaat
     5881 atttaaagca ctgagaaagt taaactcttg gtgcttttta gatataaaat tattatagaa
     5941 gaggtgtttt ttgtggcatt tatagaatgt aaatttaaat ctaaatatac tggaggagaa
     6001 actgatataa ttgtaatttt gcctgtagca aatggaaaag aactttttag tggtaaagag
     6061 atatatgatt ttgaaggtaa atttaaaaca ttatacttat ttcatggttt agaagatgac
     6121 caaagctcat ggattagata tactaatgta gagcattatg ctaaaaataa aaatatagcc
     6181 ctagtaatac caagagtaga aactagtttt tatactgata tggcgtatgg acaccaatat
     6241 tttacttttg taagcgagga attacctaaa tttgttagag ctatatttcc tttatcagat
     6301 aaaagggaag ataactttgt agctggtatg tctatggggg gatatggagc tttaaaatta
     6361 gcgctttcaa aaccaaaaga gttttcggca gttgcaagtt tttctggttc gcctgatatg
     6421 attcatgagt tagaaaatca acaaatagaa accaatgcag acattctatt tcacgatttt
     6481 ggtagaccag aagacgctag aaatagtgag aatgatttac tttacatact taagaacctt
     6541 aaagaaagaa atgaagatat tcctaaaatt tatcaatttt gtggtgatag agatttttta
     6601 tataaaaata atcagatgtt taaaaaatat gcagattctc tgggtgtaga tgttgaatat
     6661 gaagagtgtg taggggggca tgaatggcgt ctttggaatt tatggataaa gaaatttata
     6721 gatataattg gatagtacac ttatggtatt aacaggtata ctataaaata tctttaaata
     6781 tgataaagac tgaaatccta aaatcaaatt atgattttaa aatttcagtc tctaagaatt
     6841 taataaaatt ttattaagtt cttacacgag ttattattgg agtaaaattg tcaactatat
     6901 ctatatagta tttactcaat tcctctaaaa tctcttcgca taaatccgta ctatgaccaa
     6961 gattaataag aaactgtttt gtaaaatgct ttattacagc ttgagaagat atatcgtact
     7021 tgtgtgaagt ataaactcta attatttcag gtttcatagc aagtatacca atacaagaaa
     7081 gtattaattc atcatgttct atagattctt tatcattaac atatcttttc atgaccattt
     7141 cggttacact tctttgatag cttaaaatag cttcttcata agctatatcc aaataaaaat
     7201 ttctatatgc ttcgtttttt agaaggaaac aaaggtcagc attcatagca agaaagaaca
     7261 tcatcatagt accttcttca tggtagttat agattaaaga ctgcatttca tttcttatat
     7321 cttcatatat tatagctcct atttcgcttt tacttgagaa gtaatagctt attagaccta
     7381 aatttatatt agctctttct gcaatttgcc tagttgttgt cttatgataa ccttgctcaa
     7441 aaaataattc tctagcagca cttataatac tagcttttgt ttctttgccc ttttctgatt
     7501 ttgacatatt tactgtgtat acctctcttt tcaatctttt tttgaaaatt tgctaactat
     7561 aaatattgta atagaaccac ctattatagg aaccaaaatt ctgtcaaata cagtaagaat
     7621 accaatcata tttaataatg ccaatataac acttactata acagcataga tagctccttt
     7681 ttttgtagca cctttccagc aaagaccaat tacaaatggt gcaaatatta cacctgtaca
     7741 taatggagct ggccaaaaca ataattctaa taaactatca aatttaagtg ctatgattgc
     7801 acctattata gctgtcaaag ctgatgcaat ttgagaatac tttagagtat tttttaaatc
     7861 attaaattct atattaggat gtaataattt gttataaata tctcttgata taatagcact
     7921 cattgaggtc attaatgaat cggcagtaga cattatggca gccaatatag cagctaagaa
     7981 taatacagat attaaaggtg gcaaagtagt cgatatcatt tgaggtaaaa cctgaacaga
     8041 tgatatattt ggaaataaac taccaccaat agctccaatt attcctatag ggaaagcaag
     8101 aaataatagt ataaatcctg caattacact acttttaact gcatttttag cattatcagc
     8161 acattgatat cttaaaaaac tttcataaga aaatattgag cctaaaacta atggtaaaac
     8221 tgtatacata aaatcactaa tcgatttatt tcctggaaag aatggttctt ttaccattga
     8281 ttgtaacata agtaaagggt tgtcgccaac taattttaag cttatataag ctactattgg
     8341 caaaaacact atacaaatag caagttgaat aaatgctgat actgcaactc cccacattcc
     8401 tgatgtataa gtatatataa tgataactat aactcctata attacagcta tatcataagg
     8461 aataccaaat gcagtagata cagaaccaaa agaaatcaat tgactaccca taatagctag
     8521 tatagatatt ggtgttacaa gactttgata agctcttaaa aatacactat gaccatatct
     8581 agtttcaata taatcagcag gtgttatatc acaagataag cttcttaatt ttcctgcgaa
     8641 aaatgcattt ataaagcaag ctaaagctgc acctatagta gaatacattc caacccaaag
     8701 tcctctttca gctccccatt gaactccacc aactaagcac cctcctccaa aagatgtagc
     8761 agcaatggtt gcagctagta taggcaaagg cagatttcta cctgcgatta ggaaatcgga
     8821 tgctgtttta actttttttg aaaaataaaa accaatccat atgtatattg cacacgtaac
     8881 aattaaaagt aacatagtct tttctccttt ataaaatatt ttaataaaat atacaaaata
     8941 tatttaaaat atattttata taaaagtagt acgatatttg ttttattggt tttagattaa
     9001 tatagctata ataatttatg atattagatg gaaattacgt aaaaatagaa aattgaaaat
     9061 tctgaaattt ttgcatattg atatttatct catagattct tttttgtaat tgta
//
LOCUS       DAELTA010000147         9028 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.147, whole genome shotgun sequence.
ACCESSION   DAELTA010000147 DAELTA010000000
VERSION     DAELTA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.147"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..825)
                     /locus_tag="KPG13_002940"
     CDS             complement(<1..825)
                     /locus_tag="KPG13_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0986639.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVI"
     gene            complement(1086..3128)
                     /locus_tag="KPG13_002941"
     CDS             complement(1086..3128)
                     /locus_tag="KPG13_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG0986640.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3280..4251)
                     /locus_tag="KPG13_002942"
     CDS             complement(3280..4251)
                     /locus_tag="KPG13_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG0986641.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4252..5229)
                     /locus_tag="KPG13_002943"
     CDS             complement(4252..5229)
                     /locus_tag="KPG13_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986642.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5448..7007
                     /locus_tag="KPG13_002944"
     CDS             5448..7007
                     /locus_tag="KPG13_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0986643.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7113..8072
                     /locus_tag="KPG13_002945"
     CDS             7113..8072
                     /locus_tag="KPG13_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986644.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8075..8887
                     /locus_tag="KPG13_002946"
     CDS             8075..8887
                     /locus_tag="KPG13_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986645.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
BASE COUNT         3116 a         1381 c         1134 g         3397 t
ORIGIN      
        1 tataactctt ttgatatgta atgttaaata aagtagttca tcttgactta catagtaatc
       61 ataattcttt tctacatgag tttttatttt atatgcacaa tcataatcct tttcatactt
      121 tactttaatt atatctataa tttcattatt tgtatcattg attttttctt tttttactaa
      181 tcgttttgca aagaatttta aatgagtcag aagcctatca taatttatat catcttcttt
      241 aaattcaact ctataataat atcttataat atttagtatg tcttttacaa ttttagtaat
      301 taacagtgat tcctttgaac ttcccttata gtttgaattt actatatgca ttgctataaa
      361 accagcttca tctactggaa atttcacatc aaattcttta tttatataat ctacagccca
      421 tttcccaatc tcatattctt ttttatgtat tctttttatc tcatgtagta aatcattctt
      481 tatagttatg ttttcatgat atcttttaat tgcaaatgca atatggtcag taagtgctac
      541 ataaatatat tcataaagtt tagtattcaa attctcctct gcataagcaa ttatttcagt
      601 actaacctca aaaaatttct catcaacctg gcttgctagt ttactaattt tatctaaaaa
      661 gctttcatct tgtattacaa aagttctttc aattttactt ttatctattt cttgaccttt
      721 ctttttttga aacccaattc cacatcctgt taatattaat tcttttttta tattttcatc
      781 taaaactaat acaacattat tattataaat ttgttgaata atcataatca atatctctcc
      841 caaaatacaa tttcccttat tcataaaaaa atacctaaat taagatacaa ttcacctgta
      901 taccttaacc taggtattgc ctgcttacca gtaacaatcc agcttgttaa cgcttatata
      961 ttgtattcaa tttatggttt tattctatta taaataaata tatatgtcaa gtaaattatt
     1021 gaaaactatt tcctattttt tagtattaat tttaacatca aaaatcaaat tttattccat
     1081 tatatttatg attctatcat catcatttca tcatagagaa tattttccaa taactcttta
     1141 gccacttctc ttgatatttg cttagtttca actaattgtt gaacactgtc tctttgactt
     1201 tgtatcgcca ttactctgta gtattctact cttgcctcaa acttatcatc attttgaatc
     1261 tttctagtaa tccaattcaa ataacgatta tagaatctga ttgctattaa ggcctcatgt
     1321 ctgttatctt catcagtatt tttatttata aatgatatag cagttttagt agaaagtgca
     1381 tatgcatggt gaaactcttt gattatttct ttattctcag atactatttt tgtaattttt
     1441 tcttttactt ctttctttac ttttctattt ataatccaca tgcttatttt taattttaca
     1501 attttaaata tagatttttg aacagatttt cttgattttg taatataaaa tctatacaat
     1561 ttaaaggctc tatcagaaat tttaccatct tctaatagtt tcaaaacagc taataatccc
     1621 tgttggtctg cttctgcaat catcatactt ataagtttat tatctggagt atttagtatt
     1681 cctttttctg agtctaccaa ctgttcattt aaatcatgta taactggttg tacttcttgt
     1741 gctttttcat tactaagttt taaaatggct tctttcaaaa ttagtttata tgcctcttta
     1801 tcagataata aaggttctga ctcttgtacg ttatttttta ataatcttgg cagtaaaact
     1861 gttgccaata ctaaacttat aagaataact tctgatgata taaataaaac agtatctctc
     1921 attggaaata catctttatc agccaaataa aaaggtatag ataaggcagt tgctagtgta
     1981 aatgtaccat gaacaccaca agtagcaact attagtgagt atttacattt agaaatactc
     2041 tcttttacaa cttcttcatc cttaaaacct aaaaaaccta caaaaaaatt atttaaagga
     2101 ttctttttag gttttataaa agaattatgc cataaatata cccatataaa tcttataaca
     2161 aataaaataa gtacaattaa agtagaaata aaagtcaatt ccaatgccat attttcattt
     2221 ttactactaa gtcctgtata tatacttggt aacaaaaaac caagcagtgt aaagacaaat
     2281 ccattcaata catactccaa aacataccaa gtattgtctg atattatcct taatttagta
     2341 gttgtatttt gtaaatgctc tttttcaatt ccatgagcta tccctgccat tacaacagct
     2401 aggattccag atactcctat ttcttctgct acaaaatata caaaaagtgg agtcattatt
     2461 tgtataacta ttaccattgg aatttcttcc aaattccact tgtgtaaact aagtcttatt
     2521 tttataatta tgtatcctaa aatacttcct actattattc ctccaaaagc tgtaatcata
     2581 aattgaatcc ctgcctcttc tattgaaaaa actcctgtaa ctgtggcaag tactgcaacc
     2641 ttaaatgcta ctactccagc agcatcattt aataaagatt caccttcaag tatactcatc
     2701 atatttttag gaaaatttaa tccttgtgta attgatttta ctgctactaa atcagttgga
     2761 gatattactg ctgccaaagc aaatgaaaca gataatggca tcccaggaat aagaaaatgt
     2821 ataaagattc cacctgcaaa cacggttatt aaaactaaac caaatgcaag aagcaaaatt
     2881 ggttttctga gctctaatag ctcttttctc gatacatttt gtccttcact gaatagtaat
     2941 ggagctatga tacatattac aaatatttct ggattaaatg aaaatgagaa tttaaatggc
     3001 aaaatagaca atataatccc tagtacaatt tgatatattg caagaggaat attactccac
     3061 tttttgctaa atacagttga aatcgttgta caaaccaata atcccaacaa tgttactaaa
     3121 attgacataa ctacctctct tttcaataat aatattatag attaaactta tatatcaact
     3181 aacttaatat taacagcatg aaatcccatc ttaatcaaaa aaattttcag tttattctat
     3241 tattcttatt atatacatca aatttaattt ttgaaatatc tatactagat gacatgcaac
     3301 aaaatgattt cttgatattt cttttaaaac tggaatctct tttttgcata tttccttaca
     3361 ataagggcat cttgtatgaa acttacatcc acttggtgga tttgttggag atggtatgtc
     3421 tccttttaat ataattctat ttttcttaat tgtagggtct tgtattggta ctgcgctcat
     3481 aagcgctttt gtatatggat gttgagaatg taagtaaagt tctcctttag ttgcaatttc
     3541 tattatagaa cctagataca taacacaaac tctatgtgca atatgattta ctacactcaa
     3601 atcatgagat ataaataaat atgaaagttt atatttatcc tgtaaatcca taagtaaatt
     3661 tataatttga gattgaatag aaacatctag tgcagaaaca ggttcatcac aaactataaa
     3721 ttctggattt aatgctaatg ctcttgctat acctattctc tgtctttgac ctccagaaaa
     3781 ctcacctggt tttctattta aatggtactt agaaagacca caactttcta caacatttaa
     3841 tatattatct tttatttcat tctttggcac taaattatgt tctataactg cctcggatat
     3901 tatttgttcc acatttaatc ttggatttaa tgaactatat gggtcttgaa atatcatttg
     3961 cattttgggt cttaactttc taagttcttc cttctctaac ttatatatat ctacaccatt
     4021 aaattcaact ttaccttcag ttttttcaat taatctaaga atggttcttc ctgcagttga
     4081 ttttccacat cccgattctc caactatagc caaagtttct ccttttttta aatcaaaaga
     4141 aattccatct actgccttta cacatttaat ttccttatca aataatccag atttaacttc
     4201 aaagtacttt ttaagacctt ctacttttaa taaactctct tcttttttca attatatctc
     4261 ctccttttta tataaccaac atgcaatact atgaccacta ttcaattcta ttaaaggagg
     4321 tatctgagtt ctacatttat cacatacttt ttcacatcta tcactaaaat aacatgaatc
     4381 tggcatccca attggatttg gtacttgtcc tggaatcgaa tacaatcttt tatctttgtt
     4441 ttttccaaga gcaggctttg attttagaag tccaatagta taaggatgtt ttggattttt
     4501 aaataattca acaacctctg ccacttctat aattttgcca gcatacatca ctaatacttt
     4561 gtcacacatt tctgcaacta ctcccaaatc atgtgtaatc atcattatag atgtgtttaa
     4621 tttttctttt atatttttca ttatatctaa aatctgagct tgaatagtaa catctaaagc
     4681 tgttgttggt tcatcagcta taagtaattt tggattacat gataaagcca tagctatcat
     4741 tattcgttgt ctcattccac ctgataattc atgtggatat gaattatata tttcttcagc
     4801 tcttggtatt tctaccagtt ttatcatatc gattaccttt ttcttagctt cttcctttga
     4861 taaatcttga tgtatcataa taccttcaat gatttgactt ccaatagtaa acgctgggtt
     4921 taaagatgtc attggttctt gaaaaatcat tgatatctca ttccctctta ttcccataag
     4981 ttcatcttca ttcatcttta aaatatcttt gcctcgaaag attatttctc caccttctat
     5041 ttttgatgtt ggaagtaact tcatcaaaga catggctgtt acagacttac cacatcctga
     5101 ttctccaact attccaatag tttcaccttc atttatatta aaacttactt catttacact
     5161 ctttacaatc cctttatctg tatgaaaata aacttttaaa tctttaacct caattagtgc
     5221 tttactcatt attttctcct ctcagcaaaa ttacttaaga taacatacac cttttgattg
     5281 tattttataa aaatttaaat tacaacattt tcttacattt tattattttt ttctattaat
     5341 tataataata taatagttaa tttctatata tattgattta ataatgatta ctattataca
     5401 ataaaaaata tgtgttaaat attattttat ggaggtttta taagaatatg aaatttaaaa
     5461 aattggcatc attaatctta gtttcatcat taatgcttac atttacagcc tgtgcttcca
     5521 atgataaaga caagtctagt ggaagcaaat ctggtggaac tgttgtaata cctatggcaa
     5581 gtgacccaga tataatcaat ggagcttttg caaacgttaa agaagactct cttgcatcaa
     5641 atatagttta tgctcctctt tatacttatg aaaaaggaaa tttagtaaat tatttagcag
     5701 aaaaagtaga ttttaaagat agtaaggaat taactataaa attaaaatcc aatttaaaat
     5761 ggcatgatgg aaagccaata actgctgaag atgtcttatt tacatttaat acagttttag
     5821 acgaaaaaca aaattcacca tcaagacaat acttattggt gggtgagaaa ccagttaaag
     5881 tagaaaaaat tgatgattta acagttaaga ttactttacc aactgcaagt gaatctttct
     5941 tatatggtat atctaaaatt tctccaatac caaaacatgt gtttgaagga gaatcaaata
     6001 tagctaagtc agaaaaaaat aataatccag ttggttctgg tgcattcaag tttaaggaat
     6061 ggaaaaaagg tgaaagcata gtatttgaaa agaatgcaga ttattttggt ggagaatcta
     6121 aggcagattc tatagcgcta aaaataattc ctaatgaagc atctcaagaa gcagctttaa
     6181 ataatggcga aatttcactt atgaagacat ctgctgaagg atatgaaaag gcaaaatcaa
     6241 actcaaattt acaaacttat acttatagtg aagaaagatt aaactacatt gtatttaacc
     6301 aaaatatatc aaacatggct aacaaagaag taagacaagc cttatcatat gccttaaata
     6361 gaaatgaaat gatagaatca gcatatggaa aagaaggttc tgtacctgct aaatctatat
     6421 tagttccaga agcagacttc tatacagaag aaggtgttga aggatatgac caagatacaa
     6481 ataaggctaa agacttattg gataaaagtg gtgttaaaat tgataaatta aaaataggtt
     6541 ataacacagg tagatttggg cataaaaact atgccttagt agctcaacaa gagttgaaaa
     6601 aaattggtat agaagctgaa atagttcctt atgaaagtaa ggcattcttc aatatactat
     6661 ttagtaatag cactgaatgt gatatgtatg taaatggtta tgcttgggga ttagaaccaa
     6721 acccatatag aggtatgttt gaaactggtc aatattgcaa ccaaactaaa tattctaatg
     6781 ctgaaataga tgctttatgg gaaaaaggat ttactgagtt aaacaaggaa aaacgtgaag
     6841 aaatatataa gcaaatacaa caggatatat ctaaggatgc gcctatttac actatagatt
     6901 atgaacaaaa tttaatggca gctcaaaaaa atctaaaagg aataaaagat gctaaaccat
     6961 ctcctgcaat attatttgaa gattggtcta aactgtatgt agaataattg atgtaaaatt
     7021 ttatattatt aattagaaaa ggagatgtct aaaaataata ttgttttatt attttaaaac
     7081 gagacatctc ttttaattag gagaatttac ttatgttaaa attaatattg aatagagtaa
     7141 aagttttaat acctatgtta atcttaatat ctatattatc atttggactt ttagaattag
     7201 ctcctggtga cccagcagat gcatatataa atcctttaat gtcttcacag gatattgaaa
     7261 atataagggt taatatgggg cttgataaac cagcatatat aagatattta aactggttga
     7321 aaaacacttt aagtggaaac ttaggtattt cttatataaa ccatatgccc gttacagaac
     7381 aaattatgga aaaaatggga aatacattta ttcttatggg tacttctttg atattttcaa
     7441 tattggtagc catacctctt ggaatatttt tagctgtaaa taaaaattca ataactagta
     7501 aagtatcatc tgtttttaat tatataggtg tttctatacc ttctttttgg ataggtatga
     7561 tactcatttc aattttttca gttaaactta acattttccc atcaggaggt atgcatacta
     7621 ttggaaatga tagtattgga gatttagcaa aacatttagt tttgcctgta ataactttag
     7681 gtctatataa tactgctatt tttacaaatt atgtagaagc tagtgtaaat gaacaattaa
     7741 aaaaacaata tgttattaca gcaagagcaa aaggattatc agaaaaagtc atactattta
     7801 aacatgtctt gaaaaattca ctgacatcat tggttacaat cctaggcatg agtatacaaa
     7861 aattagtcac tggtgcattt gttacagaag tagttttctc ttggccaggt atgggaagat
     7921 taatgattga ttcaattttt tcaagagatt atactgtaat tatggcaata actatgttat
     7981 cagcactatt tttaatttta ggtaatttag ttgctgatat actatactta ttaattgacc
     8041 ctaaaataaa atctagcaaa ggaggattct agttgtgttt acaagtatct taaaaaaatt
     8101 aaaaaaagat aaatcctttg cttttgcatg tattactctt agcataataa tcatattaat
     8161 tatagcttct tttatattaa atatagactc taatgcaatt gatactactt ctaaacttca
     8221 agaacctagc ttaaagcata tctttggtac agacgaactt ggtagagatt acttctctag
     8281 agctctttat ggtggaagga tttcacttag tgttggtatt ttatcaatgc ttatttctgt
     8341 agtatttggt actgctgtag gtgttattag tggatatatt ggaggtaagc tagaccaatt
     8401 cttgatgaga ttagtggata tgcttatgag tataccaagt ttcttagttt tgattgtatt
     8461 aaatacttat ctaacaccta aagtttccac tattatacta attattggat tcttaagttg
     8521 gatggaagtt tcaagaattg tacgtggcga aacattaaaa ataaaggaaa atgaatactg
     8581 tctagctgct aaagctactg gtatcaaaac tagaaatata ctattaagac atatagtttt
     8641 aaacttaaaa gagactattg tagttgctgg ttcgttaaat attgctaatg ctatcttaac
     8701 tgaatctgct cttagttttt taggtcttgg aatccaattg cctcttgcat catggggtaa
     8761 tatgcttcaa gatgctcaaa gacatatttt tgacaagtta tatttagctg tatttccagg
     8821 attacttata tttttaacag tcatgagttt gactataata agcaaaaaat tttctagtaa
     8881 tacttaacaa attttatata gaaaataaaa acagtgatta tctaaagata tacactgttt
     8941 ttatttttcg tttaaaaaaa tacatatttt gtatattata actttaaata ctactctacc
     9001 atgttatgca aagcgtatta ttgctctc
//
LOCUS       DAELTA010000148         8970 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.148, whole genome shotgun sequence.
ACCESSION   DAELTA010000148 DAELTA010000000
VERSION     DAELTA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.148"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            55..1143
                     /locus_tag="KPG13_002947"
     CDS             55..1143
                     /locus_tag="KPG13_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG0986646.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1171..1962
                     /locus_tag="KPG13_002948"
     CDS             1171..1962
                     /locus_tag="KPG13_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG0986647.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2077..2631)
                     /locus_tag="KPG13_002949"
     CDS             complement(2077..2631)
                     /locus_tag="KPG13_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0986648.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            2827..4029
                     /locus_tag="KPG13_002950"
     CDS             2827..4029
                     /locus_tag="KPG13_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG0986649.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4086..5066
                     /locus_tag="KPG13_002951"
     CDS             4086..5066
                     /locus_tag="KPG13_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG0986650.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            5309..6262
                     /locus_tag="KPG13_002952"
     CDS             5309..6262
                     /locus_tag="KPG13_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0986651.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            6252..7208
                     /locus_tag="KPG13_002953"
     CDS             6252..7208
                     /locus_tag="KPG13_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG0986652.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            7205..7818
                     /locus_tag="KPG13_002954"
                     /pseudo
     CDS             7205..7818
                     /locus_tag="KPG13_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004411586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            7910..8875
                     /locus_tag="KPG13_002955"
     CDS             7910..8875
                     /locus_tag="KPG13_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0986653.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         3519 a          914 c         1509 g         3028 t
ORIGIN      
        1 aagtgtttta acaggataaa atatatcacg attttaagat aggagttgag tcatatgaga
       61 gattttagca cgtattctaa agatgatgtt atttttttat taaaagatat atcaaattta
      121 attgaagaag aaggaaataa agaaagagaa ttaaaaatac aaaatggtag acattattca
      181 gaaatgatac caatagagta tgaagttagt gaagattatc tgaatttata tcatgagaaa
      241 ctaagagaaa ataaagataa acttgcattt tctataggag ttatgtgtga aaaaataatt
      301 aaaaaaaatg gaaatgatgt agtactagtt tcacttgcta gagcaggaac acctattggt
      361 attttggcaa aaagatatat aagaagcaag tataatataa gtttacctca ctatacaatc
      421 tctataataa gagataaggg aatagatatg aatgcaattg aatatatagt aaagaatcat
      481 ccgagctcaa aaattcaatt tttagatgga tggactggta agggaactat atcaaaagaa
      541 cttgaaaaag catgtgatga acttaatgtt aaatttaaaa aatattttga ctcaacactt
      601 gctgtattag ctgaccctgc tggatattca ggactttatg gaattagaga agatttctta
      661 ataccaagtg catgtttaaa ttcaacagtt tctggtttag taagtagaac tgtactaaga
      721 gaggatttaa ttggtaaaaa tgacttccat ggagctaagt tttatagaca tttaaaagat
      781 aaagatgaat ctatgaacta tatcgaaact atagaagaat gttttaaaaa tcaatttaag
      841 aatatatctg atgaagttga aaactgggaa agtgacatta taacaagaga tggatacttt
      901 gatgttttaa atataaaaga aaagtataac ataacagata ttaactttat caaaccaggt
      961 gtaggggaaa cgacaagggt tttacttaga agagttccgt ataagattct tgttaaagac
     1021 ttaaatgata aatctttaga ccatatattc atattagcta aggagaaaaa tgttgaagtt
     1081 gaacaaatgg atttaaaagc ctataaatgt tgtggaataa taaaaaatat gaaagatatt
     1141 taggaaatat attagacagg agaatattga atgattgttt tttcagattt agacaggagt
     1201 atcatttact ccaataaatt tttaaatgct gatagtaaat atactaatat agaaatttat
     1261 agggaaaaag aaatatctta tatatcttta gatactataa atttaataaa gcaaatacaa
     1321 tattatggta tgtttatacc tacaactact agaacagtgg agcaatttaa gcgtatagaa
     1381 tttaataaat atgggattta ttttccttgg agtataactt caaatggagg agttatactt
     1441 aaagataatg aaatattgaa atcatggagt gaaaaaatag ataaactaaa atgtgattat
     1501 gagcccattg aaagtatgat tcataaattt aaagactatt tgaatgtaga tggaataact
     1561 aattttaaag ttgcagaaga tacatttttt tatatagttg ttgatttatc aagatttaat
     1621 ttggattcaa tcaaagaata tacaaatata ttggaatcaa aaaattggaa gttttatgtg
     1681 agtggcagaa aaatatattt tataccaaaa gaaatttcaa aagaaaatgc tataaaatac
     1741 ttgactaaag aattaggaat agaatatttt tatgcagttg gagattctat tatggactat
     1801 ggtatgctta atatctcaaa taaatcctat gtattgaagc atggtgatat aaataaaaat
     1861 gaaatagaaa attcatttat atcaagttct ttcaatggaa tgagtggaac tgaagagata
     1921 ctatctaaca ttttagacga gaactgccta cttaatatat aatgactgat atataaataa
     1981 taaactagaa aaatctgaaa taaattatag taaagtttat catactaatt tatttcagat
     2041 ttttatcaat ttatatttaa gtaagaactc aatatgttaa aatgaaggtg gagtcattgc
     2101 ccagactgag atacatacct catcatcata attttcatac acatgtggta tagtactgtt
     2161 aaaataaaaa ctatctcctt cctccaatag atattcctta tttccaagtg ttatcttcat
     2221 tttacctttt attatatagc cacattcttc tccttcgtgt gtcacaaatt cactgttttt
     2281 ttgtgtatga ccatctagtg taacaagtat aaattctatt tttttcccag ataaattagg
     2341 aacaagaagt tcataaaatt ttgtagaatc attagttact attttttttc gattattttt
     2401 ctttactact atatcagagt cttctcttcc aatctcttca aaaaaataac ctatatttat
     2461 atcaagagct tttgatattt tccacattgc tacaatagat ggaacaacca tattacgctc
     2521 aatttgactt attaagcctg tacttaaatc tgaatttttt gctaaagtag agatacttat
     2581 atttttagat tttctaagag atctaatttt gcttccaaca tctatattca ttagatacaa
     2641 accccctcag acatgattat aacatataat ttataaagat taataaaata aaaaaaaagt
     2701 tttacaatac aagtattttt tatgctatat tgaattttct gaaaagatgc attataatag
     2761 tattaatatt caatatattg aatattgata acgatatttt aatttaatga aatggaggta
     2821 attaagatga agagttttgt aagtcctgat aatatttggg tattatgggc agttgtaact
     2881 ggatgggcag catttagtat ttatttggaa caaaagtaca attgggcatc aaaagtgtcg
     2941 ggagcaataa tagctcttgt aggagcaatg ttattatcaa atctaaatat tataccagta
     3001 gagtctgttg tatatgacca agtatgggga tatgttgttc ctttagccat agctttattg
     3061 ttatatcaat gtaacattaa aaagatatgg aaagaaagtg gaagattgct cattatattt
     3121 cttgtaggtt ctgtaggaac tgtacttgga gctatgattg gatttttagc attaaaaaat
     3181 gtagtaccag atttaaatat tgttgcagct atgatgacag gttcttatat aggtggaaat
     3241 gtaaactttg ctgctatgtc aggggcgttt gatgcgccag gagaattagt atcagcaaca
     3301 gttgtagcag ataatctttt aatggcatta tatttctttg tcttaatagc tataccatca
     3361 attggatttt ttagaaaaca ttttaaacat ccacatgtag atgaaatgga aagtataggt
     3421 ataactgaag gagaaacagt agcagcaaat tattggggaa gaaaagaaat atctttaaaa
     3481 gatatagcaa tagccattgg tagcgccttt ataattgttg cagtatctgt agaactatca
     3541 acttggttta aagctataat accattatca aatccttttt tagcaatgtt aaatacttta
     3601 tttggaaatc aatatctaat aataactact ttgactatgt tgtttgccac atttatgcct
     3661 aatttctttg gtgatataaa aggagcacag gaattgggta catttttaat atatatattc
     3721 tttgttgtag taggtgttcc tgcttcaata acattaataa ttcaaaaatc accattacta
     3781 cttttatact gcggaataat ggtactaata aacatgctag ttacatttat agttgcaaaa
     3841 atatttaagt ttagtttaga agaagctatt cttgcatcta atgcaaatat tggtggtcct
     3901 acaacagcag cagctatggc aatatcaaaa ggatggtcta agctagtagg gccaatactt
     3961 atagttggaa catttggata catagtcggt aattattttg gtcttttagt aggaaatatt
     4021 ttgatataat tttacgtgaa aaaaagttgt ttattagaaa gatttttatt taattggagg
     4081 ttgttatgat tttcgatgga cattcagata cttgggcaga tgtaacaata agaaggctta
     4141 acggggaaaa agatgtattt agaagaatcc atcttgataa ttttaaaaga gcaaatatag
     4201 aaggagctat ttttgtaata tggatagacc caccatatga taaagagcct gaaaaaagag
     4261 ttaaacaaat tataagttct ataaaagatg aaattgaaga tatgaaagat gttgtaaaca
     4321 tagtaaaaaa atattcagat atagataaag gtataagtga aaacaaaata aatattttaa
     4381 ttggtcttga aggattaagc catattggaa gaaatataga tttactagat tatttttata
     4441 aagaagttgg tgcaagacat gcatcattaa cttggaatga acagaatgaa cttgctacag
     4501 gatgtttagg ggatgaaaat cgtggtttaa gtgaaatagg caaaaaagca gtcaataaaa
     4561 tggaaaaatt gggcatgata gtagatgtgt cacatttaaa tgaaaaatca ttttgggatt
     4621 taatgtctgt tgcaagtaag ccagttatag cttctcattc taatgctagg agtttatgtg
     4681 atgctaaaag aaatcttaca gataatcaat taaaagaaat ttcaaataca ggtggattag
     4741 ttggaataaa tagttttaga ggatttacta atagtaatcc taagaagcaa gatttaatac
     4801 atttggttga ccatatagac tatatggtaa atcttatagg tatagaacat gtatcttttg
     4861 gttttgattt tagtgattac ttagggaaag agactatagg ttcttttact ggaaatgcta
     4921 gtgtgccaag tgtagatggt cttgaaagca cacatacagc acagaacata gtagtagagc
     4981 ttgaaaatag aggttataaa aagaaagata tagagttaat ctctagtggt aatttttata
     5041 ggctctttaa agatatacta aaataatata ataaagaact taaatttata aaatagtata
     5101 tttatacaaa taatatctac atatttaaat tggcgttcat aatttattaa attttaatgt
     5161 tactagaagt ttcaattcta gtaacatttt ttatgttaaa aacagatata gatatgtatt
     5221 gacaatgata attattattg ataaactata aaagtgaaaa tgataataat tttcatttta
     5281 attaataaaa gatttttaag gagggagaat gaagaaaagg tatttagtca taggaataat
     5341 aattttgtct tatttttctt tatttatagg agctgaagat ataaatataa tgcatatatt
     5401 tgccaaaaat cagcataaac ttatgatatt tatcatgagt agaataccaa gattaataag
     5461 tatattgatt gctggtgtag gaatgagcat agcaggtctt ataatgcaac agataagtaa
     5521 gaataaattt gtatcaccta caacaggtgc tacaatagat gcagcacaat ttgggatagt
     5581 aatatgtatg cttttagtac caactgcctc tatttttaca aagacaataa tagcatttgt
     5641 attttctctt gtagggactt ttacgtttat gaaaataata gggaagttac aatttaaaaa
     5701 tataatcttt gtaccattag taggtataat gtttggaaat ataatagggt ctatgacgga
     5761 ttttatcgcg tataaatacg atttgagtca aaatgttagt tcttggatgc aaggtgactt
     5821 ttctatgata cttaaaggaa attatgagat attatatatc acaatacccc ttataatatt
     5881 agcatatatt tatgcaaata aatttactgt tgttggtatg ggtatggatt ttgcgacaaa
     5941 cttaggtctt agttacaaaa gaattgtcaa tataggtctg ataatagtag cacttgttac
     6001 agtatgtgta gtcgttacag ctggtaatat cccttttata ggtctaatag ttccaaatat
     6061 cgtatccttg tacatgggtg ataatataag agcaagcata tggtatactg gtttacttgg
     6121 agcgatattt gtacttatat gtgatatttt tggaagaatt attatttatc catatgaaat
     6181 atctataggg cttacagttg gagttatagg aagtatacta ttcctttatt taatattaag
     6241 gaggaatgtg aatgaagctt agtctcaatc aaaaaattta ctttgtaagt atattactat
     6301 tcatatcaat ggcacttttt ataatttatg gaatagacat gaatcattta gaatatgcat
     6361 tatcacaaag aataccaaaa atatttgcta tggtattagg tggaggatgt atagcattta
     6421 ctactgtagt gtttcaaaca attacaaaca atcaaatatt aacaccaagt gtactaggtt
     6481 tagattcatt gtatgtaatg attcaaacta taatagtatt catatttggt tcaagcagta
     6541 gtctgattat aaatgaaaat tataatttta ttattaatgt agctatgatg attggagctt
     6601 cattgttatt atataagacg ttatttgaaa aaaataaaaa taatattttc tttttaatcc
     6661 tagtaggaat gatatttgga accttattta aaagtgcaac aacatttatt caggtaatga
     6721 tagaccctaa tgaattttta gctttacaaa ctagtataat ggcaagttta aacaatataa
     6781 acactaatgt attgttaata gcatttataa taattattgc cataatacct tttatatatg
     6841 atgaaataaa gtatttagat gtattgtcgt taggtaaaga acaagcaatt aatttaggtg
     6901 tagattttga taaagcagtt aaaaaaatga taatattaat agcaatttta gtctcaatat
     6961 caacagcact tattggacct atgacatttt taggactttt acttgctaat ataacaagag
     7021 aaatttttaa aacatataag catacatact tgatttcggg ttctatgttg atagggatga
     7081 ttacattgat aataggtcaa ttttttatac aacatgtatt taagtttgat acaactttaa
     7141 gtgtagtaat aaattttata ggaggaattt attttattca attactatta aagggggcaa
     7201 atagatgata gagataaaaa atatatttaa aagatataag aataaaaatg ttgtagatga
     7261 tgtctctttt agtatagaaa agggaaagat aacatctttt ataggaccaa atggagctgg
     7321 aaaaagtaca gtactatcaa tagttacaag acttatagga ggtgatggtg gagaagtaat
     7381 aatagaagga aaaagcttga ctaattatag caataaagaa ttagcaaaaa aaatagctat
     7441 tcttaaacaa tctaacaata taactttaaa gcttacaata agagaattag ttggatttgg
     7501 aagatttcca tatagtgaag gtaatctaac taaagaggat gaaaattata ttgatgaagc
     7561 aatagaatat atgaagttga ctgatataca acataagtat ttagatgaat tgagtggagg
     7621 tcaaagacaa agagcttata tagcaatggt aatagctcaa gatacagagt acatattact
     7681 tgatgaacca ctgaacaatt tggatatgaa ccattccgta cagatgagat agtaaatgaa
     7741 aaagttttag aagatattta tgagatgaat tttaacatta agaatataaa tggaaatcga
     7801 atctgtattt atttttaaaa tattgtatat gaaaataaac ttgctatatg attttttata
     7861 aagtttttaa attaaaataa agattaatag gagagaggaa aatattgata tgaataaaaa
     7921 agcagcaata gtagcagcag tagctattat aggattagtg acagtgtttg cattaggtgg
     7981 aagtaaaaag aatgaatcaa aaacatcaga aaattcaaat aatactatga agataactca
     8041 taacttggga gaaacagatg taaaattaaa tcctaaaaaa gtagtggtat ttgattattc
     8101 ggctttagat acaatggatg cattaggtgt agctgagact ttagtaggac ttccaaaagc
     8161 gagtttacca gcatccttag aaaaatataa ggatgacaag tatgcagatt taggaggatt
     8221 aaaagaacca gatttagaag gtataaagtc ggcaaatcca gatttaatta taataaatgg
     8281 aagacaagaa gatttttatg agcaattgtc aaaaatagca ccaacaataa gtacaagtaa
     8341 agatgataag aagtacttag aatctgttaa aaataatata gacaaaatag caaaaatatt
     8401 tggagtagaa gaaaaagcaa atcaagaatt tagtaaaatt gaaaagaaaa tagaaacttt
     8461 gaataaaaaa gtaacagata aaaatttaaa tgctttaact ataatggtaa atgaaggtaa
     8521 tttaagtgta tttggtgaag aatcaagatt tagtatatta tacaatagtt ttggatttga
     8581 aaataaagat aaaaatataa aagaatcaag tcatggacaa aatattactt ttgaatatat
     8641 agctaaacaa aatccagaag ttatgttcgt tatagataga ggaatagcta ctggcagtga
     8701 tgtaaaagaa agctcaactg caaaatctgt tttgaacaat gatataataa aatctatgga
     8761 tgcatataag aatgataata taatatattt agattcacca acatggtatg taaatgatgg
     8821 tggtctaaca tctttaaata aaatgataga tgatgcttca aaagcggtaa attaataatc
     8881 cgatataacc aaaatgtatt ttttataaaa tgattatcaa ataaactagg agtttttaag
     8941 aactcctagt ttattttttg cataaaaaag
//
LOCUS       DAELTA010000149         8912 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.149, whole genome shotgun sequence.
ACCESSION   DAELTA010000149 DAELTA010000000
VERSION     DAELTA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.149"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..1033
                     /locus_tag="KPG13_002956"
     CDS             152..1033
                     /locus_tag="KPG13_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG0986654.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1415..2218
                     /locus_tag="KPG13_002957"
     CDS             1415..2218
                     /locus_tag="KPG13_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986655.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2230..3360
                     /locus_tag="KPG13_002958"
     CDS             2230..3360
                     /locus_tag="KPG13_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0986656.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            3364..4773
                     /locus_tag="KPG13_002959"
     CDS             3364..4773
                     /locus_tag="KPG13_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG0986657.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            4984..5493
                     /locus_tag="KPG13_002960"
     CDS             4984..5493
                     /locus_tag="KPG13_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG0986658.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            7400..8755
                     /locus_tag="KPG13_002961"
     CDS             7400..8755
                     /locus_tag="KPG13_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG0986659.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         3513 a          830 c         1525 g         3044 t
ORIGIN      
        1 ttagtataga gatttttaac ttttatgtta tactatttat aaaagtaggt ataaagatta
       61 aaatacataa tcaaatatgt ataagaggtt ttaaatagta ttgttaatga acttgattat
      121 tgtttataat tacaaattgg aggagataaa catgaaaatt actgaaggat atatgccttt
      181 taaaggtttt aaaacgtatt accgtatagt aggagaaaat acggaaggga agaagccatt
      241 agtattactt catggaggtc caggttctac acacaactat tttgaggtat tggataagat
      301 agctgaaagt ggtagacaag taataatgta tgaccaaatt ggttgtggaa attcatttgt
      361 agaagggcat cctgagctat ttaatgcaga tacttggata gaggagctta ttgaactaag
      421 aaaacatcta ggacttgatg aaattcatct gttaggtcaa tcttggggag gtatgcaggc
      481 tatttggtat gctattgaat ataaaccaaa aggtattaag tcttacatcc tttcatctac
      541 tctttcttct gcaaaacttt gggagaaaga acaaaaaaga agaatatcat atatgagtga
      601 ggttgaccaa aaagcattac ttgatgcagt aaatactgga gattactcta gcaaagaata
      661 taatgatgca ttagaaagat ttatggaaat gtattgtgca ggtgaagtga cagaagattc
      721 tccagagtgc ctgagaagac ctaaaaaatc aggttctgaa gcatatattg taggatgggg
      781 acaaaatgaa ttttctccta caggaactct ttctggatat gaatttacag atagattaca
      841 tgaaataaaa gagccttgtc tagtaactag tggagcaata gatttatgtt caccatatat
      901 tgcaaagact atgtatgata gaatacctaa tagtaagtgg gaattatttg agtattcaag
      961 acatatgcca ttcgttgaag aaaatgagaa atatataaaa gtcttaactg agtggttgaa
     1021 tgcgaacgat taaaaactat ataaatttat ataaaaaaat gagtgtctca aaatgaactt
     1081 tttagttcat taggcactct ttttgcatga aatcaaatat attttcatta tttgaacgat
     1141 aaaatgaggc ttatatctat tttgggaaag taatttttta ttactagcgt tagattctaa
     1201 tctaattgta tacttattga aataatagaa atatattggt gtttataact taattttatt
     1261 gttttttaga aattttttta tatattggta taattattct gtttaaattt gattaaaaag
     1321 tatattaatt ataataagaa acaattatat aaataggaat agattattat aattaatata
     1381 gatataaaaa taattataga aggaaaggca cattatggaa aaattgagag ttattgaaat
     1441 gataaaaaat aatcccaata ttatagctac aatagataat ccaactgatg aaatgaaatt
     1501 gttagctatt aaagaaaatg ggcttgtact agaatatata aataatccaa ccagagagat
     1561 gcaagaacta gcaatagata ataatattag agcaattaaa tttatagata atccaacaga
     1621 agatatgatg ataaaagcag taaatgaggg atggagtata ctggattata tcaaaaaccc
     1681 aactgataaa gttattgaaa tggctataaa tcaagcagga tgggcaatta aatatgctaa
     1741 aaatccaagt gaagaattac aactgttagc agtaagaaaa aattatgatt cgataaagtt
     1801 tataaaacaa ccttatgaaa gtgtacaaga ggaggcagtg agaataagtt atgatgcctt
     1861 gagatatata gattcaccta gttataatgc agaacttata gctataaaaa ataatgaagc
     1921 agctattagc tttataactg atttagataa aaataaaatg ttggaatttt taaaagtgaa
     1981 tattttagtg attaagtaca ttctgaaaaa agtatctaaa gatgaattag agagtgcatt
     2041 aaaagaagta ttatccaaag aagatgttga ggaaaagtat attagagatt ttttgaattg
     2101 tagtgttata gatagaaata gcgaaagtat accaatagat aaaattatgt ttatttataa
     2161 atatggaagt aagacagcaa aaaaaatagc tgtagatgag aaattaaaga ttatgtagga
     2221 gagagaaaaa tgaattttat agacacatta aaaagcgttg acttaatatt atccactgga
     2281 gaagtacctt taatagtagg tgaaagtgga ataggaaaaa cagctttagc aaataagctt
     2341 gccaaagaaa atgattggag tttaattgtt attgatggaa acttacttaa agagggtgaa
     2401 ataggtggtc ttccaactat agaatcttat gtaggagtta attctaatgg atataagact
     2461 gaaaagaaaa caacagtgta tgctgttcat aacaaattaa gagaaattga tgaagagata
     2521 tctaaaagta agacagttct tttatttata gatgagataa atcgttgtga gcatacagta
     2581 cagcaagaac ttatgaattt aattttaaat agagaaatta atggatataa actgcatgat
     2641 gatgtaaaga ttttagcagc aatgaatcca tcaagtaaat atggttcaga ttttgattat
     2701 caagtggtag atatggatgc agcccaagaa aataggtttg tttggttaaa tatggagagt
     2761 gaccatacac aatggataaa atgggcaata gatgagggta ttgaaagaaa agttatcgag
     2821 tttatttcca cttttccaga atatctgcat aaaataaatg aagatgatgt aagagccact
     2881 cctagaagtt atgagagagt atctaaaatt tataaagttt ataaagaaaa aaataattct
     2941 attcctagag cagtattttt aaatgttgta aagggtaatg taggtaaagt tattgcagaa
     3001 gaatttataa gttttattga atcaaactct gaaccattaa tatcctatga agatgttttt
     3061 ttaggagaat ctattgatga gtctattgta gaaagagtaa aaaatgagag tcatacaaga
     3121 ctttatctat cagctatgaa cattctaaaa gacttagaat taaatattaa gaatgataaa
     3181 tatgaatcaa accactatat taatagattt attgaatttt taaaaatgta tcctgtagat
     3241 ttaatgatag gtattatgaa ggatattaga aacagttata ttgaagtata taaaaaagct
     3301 atagaaaatg aagagtttgt aaaatcatat tttgaatctt atagtttaat aaggggataa
     3361 tgtatggaaa attattttga taaacaagca aaagagcttt ataataaagc aagtgaaatt
     3421 ataaatactc attctatgct aaaggcaaat cgtgctaatg agaaatttga tatagatata
     3481 ccacaagatt ttaagtatga gttttttagt cttgtagata aagtgaattt aagtcttatg
     3541 gaagaagaag ataattttta tggatatttt ttgtttcaga tgtcaagaga aataagattt
     3601 gatataagta gtcctacagg tgtgaatttt aagggagcta aatatattat atattttaat
     3661 ccaataatct ttttaacgct tgacataaag caaatggaga ctacaattaa acatgaaatt
     3721 catcatatat tatctatgca tttgatgaga gcaaaggagt taaaaggaaa gtatagtaca
     3781 ttagcaatta atatggcaat ggatatagta gtaaataaat ttctaaacaa tttaccacca
     3841 tatgcaacta ccttagagtg ggtaaattta aaatattcct taaaacttga accatatgaa
     3901 ccttttgaat attatgtaga aaagattcag actgaattag atttgatgga agtggatgaa
     3961 gatggtgagg aagatgatag tgataaatat ggagatatag aaattgatta tagccccgaa
     4021 agaactcatg atatatggga tgaatccaat gagatagatg aaaataccct tagagaattt
     4081 actgaaaaat ttattaacag ttctcaaaaa ggtaaagttc ctgcttattt agaaagtatg
     4141 atatcatcac ttaaaaatag taagggtgaa ttgccatgga atttatatct taatagaata
     4201 atgggaacag ttgaaaataa taaaaagaag actataacaa gaagaaatag aagacaacca
     4261 aatcgtttag atttgagggg agaacttaga agtcataagt cagaaatagc agttgcactt
     4321 gatgtaagtg gaagtattag tgatgaagag tttaaacaag ctattaaaga agttcttagt
     4381 atagtgaaaa actataatca tgaaattaca attatagaat gtgataatga aattagacgt
     4441 gtatataaag tgagaactga aaaagatata aaagatagaa ttaggatagg tggaggtact
     4501 agattttctc cagtctttga gtatgctaat aataaaaaga ttaatttgtt agtctacttt
     4561 actgatggta aaggtgaaaa taaactacaa gtaataccta gagggtacaa aattttatgg
     4621 gttatttctg gaagaggaga taaaatttca ttaaaagaac cttatggagc agttaaaaaa
     4681 cttactaagg ttaaaacaaa tgatgaaata ttagaaataa atgatgtaag aaatgatggg
     4741 tactcaatga atagccaagc accaatttta taggtattca caggagataa gctgtattaa
     4801 tttattttga tatgcttgtt ataaaaatct aaaaaataca ttaatcaatg gttaatgtat
     4861 tttttaattt taataatata agttttactt acatttaagt ctcaaaatgt gttaaaatat
     4921 aatatatagc agatagtttt ggcaaggtgg atttagttgt caaaactatt ttttttatta
     4981 aggatggtga tgtttttgtt taagattgat gattatataa tgtatggtat gacaggagtc
     5041 tgcaaagtca tggatataac aaatgaacga tttacaaatg gaataaaaaa agagtattat
     5101 gtattgagtc ctatttattc taataataca gtaataaaaa ttcctgtaga taatgaaaaa
     5161 gtaccaatga gaaagttact ttctaaaatt aatgtgcttt cattaataaa tgatattcct
     5221 aatatggata catcatggat agataatgaa aaattgagaa gtgagcaatt taaaaaaata
     5281 ctcagaggtg gtaaatgtga agagttaata aaattagtaa gaagtataga taataacaga
     5341 gagtatgtaa aatctattgg taaaaaaaca catcaagcag atgataatat aatgaaagaa
     5401 gctgaaaggt tattaaatga agaatttgca actattttag atatttctcc taatgaagtt
     5461 agttcatata tttcaagtca tatacctcaa taaagtgttt tagtatcctt tatttaatct
     5521 attttaagta ttatttatgt atgctggttt tggtaaaata aaatatataa ttaaattata
     5581 taaaaaataa taatgattgt attgaagaat tacagaatag tgcggagagc ctactccttt
     5641 aggtgtgaga tggatatcac ttaaagattt aaattgttat tgtagatgtt tttaatataa
     5701 aacaagatta ataagtgtat tgtaagcttt aagtgaaatt gatactataa tgagttactt
     5761 agattttaaa atatataata ttataaaaaa tccttttagg ttttgaatga ataagatgga
     5821 tactatgttg aatagaatcc atcttattta agttttattt atgttgttta tttgtaagca
     5881 ttatttttgt acatgaaaac acagtgtaaa aatatgctta gaattatgaa gttttattaa
     5941 aaagatattc gacaaaataa gactaaaaat attcttaaac ttataactaa gataaatatt
     6001 gtatatttat agtgtagcct gaaaaattgt ggatgtacga aatttattta gtattttatt
     6061 gaaacaaagt agaagaagta ctatagttta tctttgaata gattgttatt ttttagtgaa
     6121 aaagttatta ggttaataca taaatataat agcttataaa tagtttaagc atgttaaaaa
     6181 agtagttatt agagatatat agttacattg cttataagat acttaaaatg atagcattca
     6241 cttatattca gaagattaag ataaaaaatc atttttatat agtaaagcat acctaaattt
     6301 agaaaataat tgaatttata aaattaatta aatacataaa aaagtattca atcaacttaa
     6361 caaattttct taaatttaat ttaaattttt tattctatta attattaata ttattttcaa
     6421 atcaatgcag ttataagaag tgtataattt gattattata tactgcatac aaaaatatat
     6481 ttgagcaaat aagtaaagta ataaaaaaat caagatttca tatctaaaaa ctattctgaa
     6541 cattaattta atttatattt tcataggaaa tattttatat aatataattg ataaatataa
     6601 tttgaatttt tctaattatt tactgtcgat taatagattt ttttcttgca tataagtgca
     6661 tatttatggt acgatttaca tggtgaaata aaaaagggga ggtgaaaaat aaatattcgt
     6721 gaaaaagttt tcagtttgag gtattttatg aataatatat tttgaggtat tttataaata
     6781 atatattata cataaagagt atagcctcaa taaatagtat caaagcgaat atatgggtaa
     6841 ttactttaaa ataacagttt ttatgtttag attcacagtg tttattataa actaatgtgt
     6901 aagaaataag cctaatctgt gaatagatag tagtttagtt atttaagttg agaattggtt
     6961 gggtgaagta atatataaat ttaaaaaata aataaaaaca ttatttgaac aaaatatatg
     7021 ttgctttatt cgagactcct ttaaaaaagc actaaaatgt aattacataa gttgctacaa
     7081 aagggggatt ttagtttgta aaatactatc acgtaaaaaa taagaattgc atatggcgaa
     7141 ggttttttca tggtattaga tgagaatata ttaatttata actatatctg aaaatagtga
     7201 atttatataa tttaaattta tgattaagaa gtgtattaat tttattccat gagaatatgt
     7261 actctacaaa taaatgcgca attatcttaa taataattac aaattgtttt acaaaataat
     7321 aatattatat ctttcataaa agaaagaatt tggacaaaac gtaaaaaata aaaatacaat
     7381 taagaaagga tttaaaatta tgagctcaac agtaaaaaag aagggtaaat ttcctatggg
     7441 attttatatc tgctcaacaa ctttctcatt tgaaagagca gcatattatg cttcaaagta
     7501 cttaatatat atattcctta caacagctat agttcatggt ggtcttggaa tagataaagg
     7561 acaagctgct ataatgcaag ccaaccttgt tgcattcaca taccttgcac ctataattgg
     7621 ggggtatata tcagatagat ggataggagc tagatatact atacctatag gtatgcttat
     7681 catgggggta ggatactatc ttggttctat tgcaactaca gtttctatgg taaatgcaat
     7741 gataatctta gtttcaattg gtactgcatt ctttaaaggt aacgtatctg cggtaaatgg
     7801 acagcttttt gatagtcaag aagaacttga tacagcattt tcagtacaat attcttttgt
     7861 taatataggt tcttttattg gtactatagc agttggtatt ctttacctta aaacatttgc
     7921 aaaaaatggt gtacttggat tttctcaatg tttctttata gcagctgtac tttgtgtaat
     7981 aggtgcaata tggttcatat atggatggag attccttggg aatgcaggaa agagaccttt
     8041 taaagaaggt gtagttgctg aaaaaattga agaaaaagat aagtctcctc ttacaagcat
     8101 ggataagaag agaatttggg ctattatact tatatcattc ttctctgtaa ttttctgggt
     8161 attctggtat ctaacttatc ttgcagttta tgattatggt gcagcatttg taaacatgaa
     8221 tgttggagga tttgatgttc ctcttgcttg gtttgactca ttaaattcac ttgtatgtat
     8281 agtattagga ccagttcttg gagcattatg gtttaaactt gctagtagac cacaaggaga
     8341 tatgagttta tttaaaaaga caggtcttgg attaatattc ttaggtcttg cattcttaat
     8401 gcttgttggt gcagaatttt caagaggagt tggagctcct gaaactgcta aagctagtat
     8461 actatggata atcatgtttg gtattctttt aagtcttggt gaaatgttat tctcaccact
     8521 tggtaactct tttgtaagta agtatgcacc taagaagctt ctaggtgttc ttatgggagt
     8581 atggacattt gctactttta tagctggtaa aggatatgga tatatatatg catttactct
     8641 taaatttgat atgattaaag tatatactat aattccagta atactatttg ttgcagcgat
     8701 attattattc ttatctgata agacattaag tagacttgtt gaggacgata aataatcagt
     8761 aagcaattat tatgttttat aggttaatct actgtaatta tgttgtatta gattgattta
     8821 taactaatta ataataaaaa taaaatttta cttctaaata caatattatg atatatataa
     8881 ttataatatt tgttagagta tgattattaa at
//
LOCUS       DAELTA010000150         8894 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.150, whole genome shotgun sequence.
ACCESSION   DAELTA010000150 DAELTA010000000
VERSION     DAELTA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.150"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1996)
                     /gene="recJ"
                     /locus_tag="KPG13_002962"
     CDS             complement(<1..1996)
                     /gene="recJ"
                     /locus_tag="KPG13_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG0986660.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNI"
     gene            complement(2009..3673)
                     /locus_tag="KPG13_002963"
     CDS             complement(2009..3673)
                     /locus_tag="KPG13_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG0986661.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3971..4468)
                     /locus_tag="KPG13_002964"
     CDS             complement(3971..4468)
                     /locus_tag="KPG13_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG0986662.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4497..5876)
                     /gene="scfB"
                     /locus_tag="KPG13_002965"
     CDS             complement(4497..5876)
                     /gene="scfB"
                     /locus_tag="KPG13_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG0986663.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6172..6318)
                     /locus_tag="KPG13_002966"
     CDS             complement(6172..6318)
                     /locus_tag="KPG13_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG0986664.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6348..6926)
                     /locus_tag="KPG13_002967"
     CDS             complement(6348..6926)
                     /locus_tag="KPG13_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG0986665.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(7294..8724)
                     /locus_tag="KPG13_002968"
     CDS             complement(7294..8724)
                     /locus_tag="KPG13_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG0986666.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         2821 a         1609 c          830 g         3634 t
ORIGIN      
        1 caatattagg agaaaatata agctctaatt tatcagtatt tttgctaaca tcttcaataa
       61 tattgtaatt tatgttaaaa tccaaatcta taagtgatat atctgacatt gctctataaa
      121 atccatttat agaattagat attattagtg tatctttttc aaaataatca aatatattta
      181 tatctgtatt tccatcaaat aattcaaaat cagacacaga tatattatac aggctttgag
      241 tatcttttgg tattattttt gacattaatt tcaatgaaag tttgtcatta cttgcacttt
      301 taggtcgagc caatcgtata tctttaagta aaaattgtac tttcctattt cccatatagt
      361 tattttcatc taattggaat agtacatcta ccttgtctcc tactttatac attgatttta
      421 aatgactcat attaaatcct atacattcat agcttttctc tttttctatg cttaatttca
      481 gatgttgttg attcttacct ataactttca aatcttttaa aatataattt ctaactataa
      541 atctaggatt aggattattt aatccaaagg gttctaactt atgtaattct tctacaagat
      601 ttaaatttat tacattttct tgtaattcaa actcaacatt tacattctct atcatatcat
      661 cttcagttaa attataatct gcaaatttat ttatttcatt tgctaaaatt tctacattgt
      721 ctgaatccaa ggataaccct gctgcttgtt catgaccacc aaacttagtc atcaagtctt
      781 tgcactttat gagagcttca aatatattaa atccttttat tgacctcgct gaaccagtag
      841 cctccccatt ttctatacct agaagaattg ttggtttgta gtatttttca gttaactttg
      901 atgctacaat tcctattatt ccatgttgcc aaccctcttt agctaaaacc aacaccttgt
      961 catcattgta tctgctatta gactttagca tttcctcagc ttcatgatac atcttagctt
     1021 ctatcatctg tctttcatta tttttttctt ccaatatact agctatttct actgcctctt
     1081 cttgtgattg agtagtaaat agctctactc ctaagtaaga atatcccaat cgacctgaag
     1141 catttatcct aggacctatt gcaaaaccta tatgagaaga acctatctta tcactttcta
     1201 caccacaaac ttttataagc tcccttaatc caatattttt gccttctttc attgatttta
     1261 aaccattttt tacaattatt ctattttcat ctataagtgg aacaatatca caaatagttg
     1321 ctagtgttac tatttccaaa taattataca tactagtttt aaattcttct tttggagtca
     1381 aggcttgaat cattttaaat gctacaccac atccacatag tgaatcaaat ggataattac
     1441 aatcttcttg ttttggattt attactgcaa aggctgatgg aatttcactt tgacattcat
     1501 gatggtctgt aattataaca tctatgccca aatcattcgc tgtattgacc tcagacactg
     1561 atgtaattcc acagtcaaca cttataatca agtcacatcc cctagagctt ataagcttaa
     1621 tagcatcttc atttatacca tacccttctt ctaatctatt aggaatatag taatcaacat
     1681 tgtaacctat actcttaaaa tataagcaca agatagatgt agatgataca ccatcaacat
     1741 cataatctcc gtaaatccaa attttttcgt tcttctcaac tgctaacttt attctatcaa
     1801 cagctttttg catatctttc attaaaaaag ggtctcttaa ataatctaaa gatggattca
     1861 taaatatctc tgaatctttt tcagtacata tccctctatt ttttaaaatt tggcagattt
     1921 ccggggaaat attaagtttt ttgctaaatt cactttcctc tatcttacct ttatacttca
     1981 atatccattt tttcaaattt aattccccct aatctttttt gtattttgac ctaatacttc
     2041 ttataactaa gcaaaggcct attataaagg atataaagaa acccgctatt gcagtaaatt
     2101 ttatccatat attattttct atatttggag atgcaattat aagagaagaa cctactataa
     2161 tagatgccaa cactaaactc aaagatattt gagatgttag ctctattata caattttcta
     2221 acctcgtcat tttaacatct tcaatttgca tttttatatt atttctttct atatttttta
     2281 aaattgcttt taattgcttt ggaatagttt ttatgtccag taatatttct tctacatttt
     2341 gtcttgaact taaaacaaca tttttagggt taaatttact cttataatga tgtttcataa
     2401 actcttttcc catagaacca aaagaaaaat ctgtgtttaa agttctactt gttccttcta
     2461 atgttataac tgtctttgca agagttacaa attgtgcagg catgactatt ttatactgtc
     2521 taaaaaacct aaatagttca tttaatatat ctgttacatt tattttttct atagaaatat
     2581 catagtaata atgtattaga taaagtaaat cctgtctaag tcccttaata tcagcctctc
     2641 catttaatgc atccatttct atcaatagat atattatctt gtcaacattc ttttctacac
     2701 ctgctaatgc aatttcattt aattggttta aagttttatt atcaataatt ccaatcattc
     2761 caaaatctat atatgctatc ttatttttag caactacgaa tatattacct ggatgtgggt
     2821 ctgcatgaaa aaatccatga gataatacct gtgtaaaaaa tgacctaacg cctatttcaa
     2881 caatagtttt tgtgttataa cctagtctcc ttattttttc tacatcactt aactttgtac
     2941 cattaacttt ctccattact aaaattttct tagtattata ttctgaatat actttaggta
     3001 tatatacttc atcactattt ttaaatatcc tagagaattt agttgcattt atagcttcaa
     3061 atgtataatc aagttccctc attaattgag tttgaaattc ttcaatcatc tgtactaaat
     3121 ctaaattaaa atcctttttt aaatctttta aagtatttgc aatagttcta agaatctcta
     3181 aatcagattt tattattttc tctatatttg gtctttgaat ttttacgatt acttcttctc
     3241 ccgttttaag tacaccttca taaacttgac caattgaagc tgctgctatt gcttcttctt
     3301 taaattctaa gaatatttct tctatactta aacccacttc ttctttaaat atatcaatag
     3361 ctatatttga atcaaatttc tctacatcat ctctgagttt tgatatttca tcaattatat
     3421 cctggtctaa caagtctttt cttgtactca atatctgtcc tatttttata tatgtaggtc
     3481 ctagttcttc taaaactctt ttcattctct caccagttgt catatttttt atttcttctg
     3541 gtgggtcaaa tagtggtatt ttataggcaa ttccttctat atttagtctt tctacaataa
     3601 aactaaaacc atactttatt aaaacatatc caatttctct atatcgcttt aaatttctat
     3661 aacttattct caaatctttt cactcctaag ataggtatac ccaaaaactt acaccttaat
     3721 ttatcgtata tgacattatt tcaccaattg atattctaat atcctatatc ctctttaata
     3781 tttaatttta tcacatatac taccaatttt attaaaatta tttaactcag ttaattttta
     3841 tttttgacat tttatatctg gtttaatcaa atactttttg aaaactaaaa tatcaacaaa
     3901 atctaataaa ataaattagt ggttgcctca aaacagattt aaatatgttt tgaaacaacc
     3961 actctttaaa ctaatatttt tgattttgtg ctgcctctaa ataccattca taagattcat
     4021 caatatattt tttatcttct tctgtaagct ctctaacaac cttaccagga gaacccatta
     4081 tcaaaacacc tggcggaaac ttcttattag atgtaattag tgtacctgct cctatcagag
     4141 tatattctcc tatttcagca ttatccaata ttattgaacc cataccaatt aacacattat
     4201 cactaatttt acaaccatga actatagacc tatgcccaac tgttacattg ttaccaatta
     4261 tagtttctgt atctccatgc actatagaac aatcttgtat attagtgttt tctccaatag
     4321 ttattggtcc ttcatcaccc ctaactactg cattgtacca tatacttgaa tctttgccta
     4381 ttttaacatt tccaattaca tctgctgatt ttgccacaaa tacactttcg tcaattaaag
     4441 gtttatcttc taaataatct ctaatcatat ataaacaact cctttaaaat ataaatttag
     4501 ttggttgctc cttctaacat taacttagct tgtatcatta tagagcactc tgtacgtttt
     4561 ctttgcattt cacatcctaa ttcatatggt tttgatatgt cattattaaa gtttatcgca
     4621 tttgcatgac atccacctga acagtaaaac ttattccaac attgtttaca ctcttcttta
     4681 gtatatacat gagcttctct aaacatattt tttaaatttt ctggaagtac tatttcttca
     4741 tcaaatatat ttgccatttt aaattcttca tttccaacaa attgatgaca tggatagata
     4801 tctccatttg gagttacaga aagatactca tttccagctc cacatccagt tattctcttt
     4861 attacacatg gaccttgatt taggtctatc ataaaatgga aaaatttaaa tccatctcct
     4921 tgtaattgtc tatctgcata ctctacagca aatttttcgt actcttcaaa tatcttaggt
     4981 aaatcttctt ctctaagtgc atatggatta ctctcatctc caacgactgg ctctacagag
     5041 gttaatttaa atcctaaatc tgcaaaatgc ataacatctt ttgaaaaatc taaattatct
     5101 cttgtaaaag tacctcttat ataataatac ttatcttttg ctcttttttc cacaagtttc
     5161 ttaaatcgtg gtaatgtaat atcataactt cctttatcat ttaaagttgg tctcatatta
     5221 tcattaactt cttttcttcc atctaaactt aaaactacat tatgcatatt ttcatttata
     5281 taatctataa tctcatcatt tagcagtaca ccatttgtag ttattgtaaa tcttatattt
     5341 ttattataat ctttttctac acttcttcca tagtcaacca gttgttttac aacttcaaag
     5401 ttcatcaaag gctctccacc aaagaaatct atctctaggt tctttctact tcctgaatta
     5461 gcaatcaaat agtcaattgc tgccttacca acttcaaaac tcataagttc tttttctcct
     5521 ccaaaatcac cttgagctgc aaaacagtat ttacatttta agttacaatc atgagctaca
     5581 tttaaacata aagcttttac tacaggctct ctatgtacaa agcttggatg atattgatat
     5641 gtatcttctg tatataaaag accctctttt tccaaattta atatttcttc atatgcttca
     5701 tttatttttt cttcttgata ttttgatttt aatatctcta atatttcttc tttacttttt
     5761 tctttataaa aatctaacaa atcgtatgca acatcatcta acacatgaac agctccacca
     5821 ttcacatcta aaacgatatt atatccattc atagaaaatt tatgtatcat actcataccc
     5881 tacctcctaa attactatct atactaaagt tttttattat tttaattaaa ctttaccctt
     5941 cattatatca taagtttaca taaaatacat acaatacaaa aatgacttac tctccgtaag
     6001 tcatttcatt aaatacacta tatcccccta aattggcgca ctaatatatt accaaatagg
     6061 tattaggctc ctataaaata aattttgtat ctgattttat attgttttaa tcttattaaa
     6121 atataataat ttcttttttc ctgataattt tgtataaata agtgcttgta tttaatttgt
     6181 cttctttaga ttgctgattt ctttgggtac tttaggctga tgaataatcc aaggacaggt
     6241 actattagct gacaaaatag cagtgctaag tgccaatgag ctagcaaact tcataaatct
     6301 tacaataaac tttttcataa cttaactctc cctcatacat attttactta tactttagct
     6361 tttaacttac ctaatactaa catcacaaaa atccaaaaca tggcaaatgc gctatatgtg
     6421 aaataatcta caaatatact caaagaaaga cttgaaattg ttatcaataa cactactgta
     6481 gatataaata tagcagtttt cttataaacc aacttttccc tatcacttat agggttactc
     6541 ctatgttcta ttgggcacag aacacaaatt ccaacccaac taacacttgc aataatcata
     6601 ataatatttt taaataagtc tatatttata ttttctataa ttaagattgt ggataaaatt
     6661 ataaaaataa aagttaataa gcatctaaaa taattgtctg catgataccc tcctgaaaat
     6721 tgtcttattg gacaataaca cattaaaaaa aataatacat atgtaaattt accaaataaa
     6781 aaacctataa acagcatggt ggttatatta actatgaaag ctattaatat ttcaaatcca
     6841 taagaataga ctttagattc attattatct atcatattat tacaaattaa aacagatgtc
     6901 attttttcag catatctttt aaacattttt acctccaaaa aaattttaca tatatagagt
     6961 acattctttt ttgagaaaat tcaatatact ttacaaaact agttgttaaa aatgtaaaac
     7021 tggtcaaaaa aagacctata taattttctt cttattattt atataacaaa acaattataa
     7081 taaaacaatt ttatcaagct ttatatgcat aaaaagccta tgtcaaaata gaaacaaacc
     7141 ttttcccctt tgttaaaagg ctaaaaatat atttcatttc atataaaaag agtacctcac
     7201 aaactaaaaa gttccttttg agatacccat tttatttatt acaaaatatc aaattttatt
     7261 tatttgcata tattccccat atattttagc cttttaactt tgtttttgtt ttacatactg
     7321 tagtgctttt ctattctgtt ttactgcttc aagacaaact ttttctgttt gattatcaac
     7381 aaattctaaa gctctataat tttgctttac tgcttctaaa catatttttt ctgtttgatt
     7441 atttacatat ttcaacattc tataatcttg ttttactgct tttagacaaa cttcttctgt
     7501 ttgttctttc acatatttaa gcgcttcata actttgatta actgcttcta aacaaatctt
     7561 ttctgtttgt tcctttacat aagctaaagc attatagttt tctcttactg cctctaaaca
     7621 tattaattct gtctgatttt ctacatattt aagaattttt ccattttgct ttactgcttc
     7681 tacaaatatt ctttctgttg gtatctttac ataacttaat atttctttta tttcaagttc
     7741 ttcaaatgaa cctcttactg cttctataca tatattttca gtctgttttt ttacatattg
     7801 cagtactttt ccattttgtc taactgactc caagcaaatt tcttctgttt gctcttttac
     7861 gtactgtaat gctcttccat caattcttac tgcttccaaa caaatctctt ctgtttgctt
     7921 atttacatac tgtagagcca tataacttct ttttactgct tccatgcata ttttttctgt
     7981 ctgttcattt acataataca atgcaaagtc attctgtttt actgcttcta tacatatttc
     8041 ttcagtttgc tcttttacac aagatagtgc actgtagttt tcttttacag cttctaaaca
     8101 tatactatta ctttgttcct ttacataagc taaagatttt ccatcttttt ttacagcttc
     8161 taaacatatg gctggagttt gctcttttat tccttttaat atctctgcaa catcattttt
     8221 agatgccctt tctataaatg ctagacatac atcttctgtt tgctcttcca caaatttaaa
     8281 tacttccatg acatcataag ttgatgcatt ttttatagcc tcaattagta cattttctgt
     8341 cttattattt atgtagtaca aagctctgta gttctgtttt actgcttcta tacaaatatc
     8401 ctcattttga tttgttatat acttaagaga tttatatgtc tgttttactg cttccataca
     8461 tactttataa gtcttgtcct ttacaaactc aattatagta ccatcctgct ttactgctag
     8521 cagacacatc tcttctgttt ggttttctat gtcttttaac tctctgtaat catattttac
     8581 tctgcttaaa tcatttaaat gtactttgtt atcttccttt aaatcttctt cattattttc
     8641 tatatcatac tcaaattctt catcatctaa atagtcattt ttttctggta aaaatataga
     8701 tttaatttta ttctttatat ccataaaatt atcctccttt aatatgattc tatcattaaa
     8761 gtaaatcgtt tttaagcact tatttaatta attttatatt aagtttattt aaaagtctat
     8821 tcattgtcga tagattatat tttatattaa taattttatc ttatatatgt ggggtaataa
     8881 agttaatagt ggac
//
LOCUS       DAELTA010000151         8879 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.151, whole genome shotgun sequence.
ACCESSION   DAELTA010000151 DAELTA010000000
VERSION     DAELTA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.151"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            93..434
                     /locus_tag="KPG13_002969"
     CDS             93..434
                     /locus_tag="KPG13_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG0986667.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            585..998
                     /locus_tag="KPG13_002970"
     CDS             585..998
                     /locus_tag="KPG13_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG0986668.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1200..1841
                     /locus_tag="KPG13_002971"
     CDS             1200..1841
                     /locus_tag="KPG13_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG0986669.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1865..3118
                     /locus_tag="KPG13_002972"
     CDS             1865..3118
                     /locus_tag="KPG13_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0986670.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3164..3814
                     /locus_tag="KPG13_002973"
     CDS             3164..3814
                     /locus_tag="KPG13_002973"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG0986671.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3865..4461
                     /locus_tag="KPG13_002974"
     CDS             3865..4461
                     /locus_tag="KPG13_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0986672.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4474..5313
                     /locus_tag="KPG13_002975"
     CDS             4474..5313
                     /locus_tag="KPG13_002975"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG0986673.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5558..6712
                     /locus_tag="KPG13_002976"
     CDS             5558..6712
                     /locus_tag="KPG13_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG0986674.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7018..8715
                     /gene="ade"
                     /locus_tag="KPG13_002977"
     CDS             7018..8715
                     /gene="ade"
                     /locus_tag="KPG13_002977"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG0986675.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3635 a          853 c         1575 g         2816 t
ORIGIN      
        1 cttaagtaaa tcagtcttac atatatttcc gatagattat attgataaaa aacactaaaa
       61 ttaaattaat aaaagtactt ggagggatgg aaatgaaaga ggaaaatttt attaatgttg
      121 aatacgagga taaacgtttt tcaattaata gtaaagaagt tttgtacata gaaaagcaag
      181 agtatgaagt aaagatttat actgaaagtg gtaattatga agttggagaa aatgctcctt
      241 gtataagaaa actattgaaa gaagaaaatt ttttcaaatg cagtaatagt tatattgtaa
      301 atctagacaa gtgtgaattt tatctcagca ataattgtat ttttgtgaat aataatttta
      361 taagaatttt aggttcagaa agaaaaaaga aattaaaaaa gaaattagaa aaattatatg
      421 attatgaaga ttaatattgg gaggctgtat tttatacagc ctctttattt attgattgta
      481 ttattaataa attttaaatt atgataaaat ataaaacaag taacttattt cagaataaaa
      541 tactttttaa aagaaaggga cgagatatgg gagattataa taaaatgctt gaattaaaga
      601 ataacttagg tatcagtgaa agaaagttaa aatatccttg tatctataaa catttcaaag
      661 ggaaatatta tgctacaatg ggtcttagca aggctattga tgatatagaa aacatatgtg
      721 aaatatatgg taaagaaaat ttaatccaaa acagaaataa atataaatta gttattagac
      781 atactgaaag agaagaagat atctatgttt atagggattt agatggaaac ttttatcata
      841 aaaaagaaga agatactaat gatttagttt tatacaagac cttatatgat gatacaggaa
      901 tatttgcaag acctttggat atgtttttag aaaaagtaga tacagataag tatgtcaatt
      961 caattcaaga atacagattt gaagaagtat ataaataata taagagaaat atgtttataa
     1021 atttagaaaa attctttaac agtacaataa tttgcagaaa tacaaatgag atttattgta
     1081 aataatatgt tagattagat tataaaatag aaacaaagta aagtattata tgatataatt
     1141 aagttttgag aagtgataat ttatatttaa taaaaaatag gcatatatta ggaggacata
     1201 tgggaaatac tttttatata gtaagacatg gacaaacgga ttggaatata ttgggaaaaa
     1261 cacaaggtca tggtaattct gaccttacgc ctcaaggtat agaacaggca aaagaacttt
     1321 ctgaagatat tggcaagtat tcaattgatt atatttttag tagcgacctt gggagagcaa
     1381 tgcaaacagc tcaaatttta ggagataagc tcaatattga agttcaaaaa actgaggcac
     1441 ttagagaaat gggattcgga gtttgggaag gccttttaat aaaagaaatt caaaaagatt
     1501 attcagatat atatgcaaca tggagaaatg aaccacactt agtaaatata ccagaaggcg
     1561 aaactcttaa gattataaag gaaagagtag atgcttttat aaaggaacta aatgaaaaat
     1621 atgataataa aaatataatt cttgttacac attcaattac attgagggtt atgttgttgt
     1681 catttttaga atcaggtatg gaaaatatat atagaattaa gcaagataat actgctttaa
     1741 atatagtaga atttaaagat tatgggcctg ttatagtaaa aatgaatgat acaagtcata
     1801 taaaaaatca tgtaaagata aataattctg cactggagta ggaatcaatt ataaggagaa
     1861 atttatgagt aaagttatag tagttggtgg aggagcttct ggaatgatgg cagctttgtc
     1921 agcttcaaaa aacaataatg aagtaatatt agttgagaga aatggagaat taggaagaaa
     1981 acttagggca actggtggcg gtagatgcaa ttttaccaat aatagagaga tagaagattt
     2041 ttttgataaa gttgtcagta ataaaaaatt tctctatagt agtttttata catttacaaa
     2101 taaagacctt atttcgtatt ttgaaagtag aaatttggaa tataagattg aagaagaaaa
     2161 tgaccacaaa gtatacacta aaaatgataa atctatagaa gtaatagaag tattaaataa
     2221 agatttactc aatcacaatg taaagataat gtataataag aaagttatag atattataac
     2281 tgaagaaatt gctctaaaag atgattctaa taaggacaag tcaaagtatc taataaaagg
     2341 gattatttta gataatggag ataaaatact aggagacaag gttattatat caactggagg
     2401 agtgagttat tcaaagacgg gttcagatgg ttcaatgtat aagattctta aaaagcatgg
     2461 acatacatta aacaaactat atcctgcttt agttccactt acaatagagg aaaaatggat
     2521 taaagattta caaggaatat ccatgaagaa tgtggaaata tcttgtaaga ttaagaaaag
     2581 gaaaatatca aaatctggag acatgctatt tgcccatttt gggataacag gaccttgtgt
     2641 tcttataatg tcttcttata ttaacaagat tattgaaaag gaaaaggtag aattaaatat
     2701 agatttctta ccaaatttaa gtactgatga aatctctagt ataatcagag catttccaaa
     2761 taaaaatgtt ctaaataatc ttaagcaaat actacctcaa aatttcttaa aagagatttt
     2821 ttctctatta tctttagttg ataaaaaggc aagtgatttg tctaaggcag atgaaattag
     2881 aattattgag tatataaaaa atatgaagct aacttgtaat gggactacag gtataaacac
     2941 tggtatggtt acttctggtg gtatttcagt taaagaaatt aattcttcta ctatggaatc
     3001 taaattagtt aagaacttat tttttacagg tgaagtaatt gatatagatg cagaaactgg
     3061 tggatataat ttacaaatag cattttcaac tggatatctt gcgggtataa gtgtataaat
     3121 ataatttttt aataattaaa tatgaataaa tagaggtgta tatatgggta atttagttat
     3181 agctgtagat ggtccagcag gagctggaaa aagtacaata gccaagatag tagcaaaaaa
     3241 gctaaatata aattatatag atacaggtgc tatgtacaga gcagttactt ataagtgttt
     3301 aaaaagtggt attgatgtca ataatgaaaa agaagttatt caaatagctg aaaattcgga
     3361 tatagatttt aaagataaca atatatattt ggacaaagaa gttataaatg aagaaataag
     3421 aacaatagaa gtaagcaata atgtttctaa tgtagcaaaa ataaaagaag taagacagtt
     3481 gatggttgaa gttcaacgaa aaataggaat gaaaaattca gtaatactag atggtagaga
     3541 tataggttca tatgttttcc cagatgcaga ttacaaattc tttttagtag ctactccaga
     3601 agaaagagga aatagacgct ataaagaact ttgtaataaa ggatataata ctactttaga
     3661 agaagtaatt gaggatataa taagacgaga tgaaattgat tcaaatagag aatttgcacc
     3721 attggtaaaa gctaatgatg cactagaaat agatactaca ggaaaaacta tagaagaagt
     3781 tgtagaagaa gttgtatcta aaataaatct ttagtttttt atgcatcata atgaaaataa
     3841 atgtagtgtt gaagggagat aaacgtgaat ttttatagat ttgtaataaa tatttttaag
     3901 ggtttttcta aaatattttt taaatatgaa gttataggtg cagagaatat tccagataga
     3961 ggaaatatag taatagcatc aaatcataaa tcgaatcttg accctatatt cttggctgct
     4021 gccatagaaa atagagaaat agctgcaatt gcaaagaaag aattgtttaa agtaaaacca
     4081 ttgggattta tattaaaaaa attgcatgtt atgcctataa atagagaaaa accagatgta
     4141 tctacaataa aaactatttt aaggtcagtt agagacggtt atgtattagg aatatttcca
     4201 gagggtacta gaataaaagg agattctttt ggtaaggcta aggcaggtct ttctgtattt
     4261 acaataaaaa gtaaatctaa agttgttcca gtatcaataa tatctaaata taaacttttt
     4321 agtaaagtaa ttgtatatat tggagaacca atatcatttg aagaacattt taaggaaaaa
     4381 ttgagtaatg atgaccatga aaggatatca caagagattt tagaagtgat aaaacaaaat
     4441 tactttaaat attcaaaata agggggtaaa gttatgaaca taaaaatagc aaaaaatgct
     4501 ggcttttgtt ttggagtaaa aagagcaatg aaaatggcat gggatgaagt tgaaaaaaat
     4561 gattctggca tatatgcttt aggtcctctt attcataaca agcaagcagt agcaaaatat
     4621 gaagaaaaag gtcttaagac tgtgaatgaa attgatacaa taccaaatca tgaaaatatg
     4681 ataataaggt ctcatggagt tcctgaaaat atatataagg aagccaaaga taaaaaatta
     4741 aaaattgtgg atactacatg tccatttgta aagaaaatac atacagtagt aagtgaatat
     4801 cataataaag gctatgaaat aatagttatt ggagatatga agcatccaga agttataggt
     4861 ataaatggat ggtgtgaaaa ttcagctata ataataaaga ccttagaaca aatggaaaat
     4921 atggaatttg ataactccaa aagatattgt cttgttgcac agactacaat aaatccagag
     4981 ttgtatataa gtatagttaa taaactatca gataaattag aagaaatagt atttaatgat
     5041 acaatttgtt cagctacaaa aacaagacaa gaatcagcta aagagttagc taaagaagtt
     5101 gattgtatga tagttatagg aggtaaacac agttcaaata ctcaaaagtt agtaaaggtt
     5161 tgtgaagact tagtaccaac ttttgcaata gagactaaag atgaattgga tgtaaatacg
     5221 cttaaaaaat ataaaaattt gggtattaca gcaggggcat ctacacctaa ttggataata
     5281 gaagaagttg taacattcct tgaaaattta taatttgata ggtaataaaa ataggtggac
     5341 attgttataa tttatcataa tatccacctt tgatttcata ttacatgtgg taaatagata
     5401 acctaatatc aaaaagtgtg tatcttaaga tgaaaaacta agagtataag atgcgcactt
     5461 tttaatatta gaaattcaaa agaaatattg atattgatta tcaatagaaa atagaatata
     5521 atataattgt tgcaaaatta gtctaggagg taaattaatg gaaatgttga tatccgcttc
     5581 tgaagaggcg tttttacatg taggttctat gattggattt tttattttgt tatttggata
     5641 tattaattat aaaactagtg gaaattttac taatatcata tcaaaaaaca gaaaatttca
     5701 acccttaata ggtgctttaa ttggagctat accaggttgt ggcggaagtt tggcaataat
     5761 gccattgtat ataaatggaa aattaagctt tggagcaata attgcaagtt taatagctag
     5821 tatgggagat gcagcatttg tacttatttc atcaaatatt aaaatgtatt tttttgtcac
     5881 tatagttagt acaattacag gaataataac tggacaatta gtggattact ttaaactaga
     5941 agaaaaatta gggttaaaaa accgtagaaa atcagataaa tatgatataa gtaaaaataa
     6001 ccctaaagaa aataaagaac aacataatca tgatgaaata attttagaca ctctagctaa
     6061 atctcatgga aatacaaata gacttgcatt tataataaca catggaaagg gatataaaat
     6121 ttatatagga attatagtaa tcggttttat atttatgtca cttgctcact ctggacttaa
     6181 tcttcctata attgagaagt tacattcatt ggaagaagta atagcagtga ttggaatatt
     6241 attttcaata atttatatgt ggtgttttaa aaaggtattt aaaaattcta atcaaaaaga
     6301 ggaagaaaat aaaaagattt ctttgcgaga aatgcttatc cattctgttg gagaaatatc
     6361 atttgtaatt acatggattt ttattgcata tcttatttac gatttgataa tattaatttt
     6421 aggtggagat gaatacttag ttaaattagt attatcaaca ggtgtaataa gtgtatttat
     6481 aggtgcaggt cttggactta ttccaggttg tgggatacaa attgtgctta tgtcatttta
     6541 tttgaaggga actataccac ttggtgcagt gatagccaac tctatttctc aagatggaga
     6601 tgcacttttc ccacttttag caatggataa aaaatcttct ttatgggcta tgattattac
     6661 tactatacca gcaatacttg taggtatgat agtttatgca tttttaggtt aaatataatt
     6721 atgaataaag aagtattaaa aaaataataa agcacataaa tttatttata agaatagtga
     6781 agattaaata taaaaataaa aaattcaata tgaattaaat tattgcttaa atattaatga
     6841 gaacatggga gataatatta ttcctcatgt tctctctatt taattaaagg aattatggaa
     6901 caaatttcaa gtgatacttt ctatcaaaac tcatatataa tatatattaa taaattaaac
     6961 tataatacgt aatacagtca gtatattgat ataaaaaact taggaggagt tgtaaaaatg
     7021 ggtaatttaa aaagacagtg tgatgtttca agtggaagag aaaaagctga cattgtacta
     7081 aaaaatggga caatcataaa tgtatttaca gaagagttaa taacaggaga tgtagcaata
     7141 gtaggagaca ctattgttgg aattggcgat tacaagggca atgtagaaat tgattgtagt
     7201 aacaaatata tatcacctgg ctttattgat gcacatatgc atattgaatc tacaatggta
     7261 atgccaattg aattatcgaa aaaattatta aaatcaggta cgacaactat catagccgac
     7321 ccacatgagt tggtcaatgt aaaaggagtt agtgcaatag attttttatt agaatcgaca
     7381 aaagatatac cacttaatgt atatattatg gtgccttcat ccgtacctgc aacaagtttt
     7441 gaaactaatg gagttggtaa atttagtgca aaagacatgg agagttatgt aaataatcct
     7501 agaattttag gtttaggaga ggtaatgtgt tttaatgatg ttataaattc tgaaaatgaa
     7561 atcttggata aactagagct ttttaaaaat aaagttgtag atggacatgc acctaatata
     7621 aatggtaaat cacttcagac atatgtatgt gcaggaatag aaaatgacca tgaatgtata
     7681 acttttgatg aagtatatga aaaattgaga gcaggattaa aaattttaat aagagaagga
     7741 agcgcagcaa aaaatttaaa gtctatagta agtggtatgc taaagcataa cttgccaatt
     7801 gaagaattta tgttttgcac agatgataag cacttagatg atatagaaaa acaaggtcac
     7861 ataagatgga atataaaatg tgcgattgat ttaggtatgg aacctgttag ggctataaaa
     7921 gttgcaactt ataattctgc aaaagcatat gggttaagga aaattggagc aataggtgcg
     7981 ggttataagg cagatattgt tgtattgaat gatttagaca aaatggaagt ggatagtgta
     8041 tataaagatg gtaatttggt aaacgaagag atgttttcta attacaatta tgagattaaa
     8101 gataaagaat tgcttaatac agtcaagttt aaatatatta ataaagaaaa aatacagtta
     8161 aaagtaagtg aaaaaaatta tgtaatggag attgtacctt atcaaatttt gacaaataag
     8221 gtatatgaat ctttaccatg tgcagatgga tattttgttc caaataaaga gtattcaaaa
     8281 ctttgtgttg ttgagagaca tagaatgact ggaaatgtag caattgctcc actaaaagga
     8341 tttggcataa aaaatggagc aattgctact actgtggccc atgattctca taatattata
     8401 gttgcagggg ataatgatga tgatattcta gtagctataa attacttaaa ggaaatacaa
     8461 ggtggttatg taattgtatc aaatggaaaa gttcttgcac acctaagtct tcaagtagct
     8521 ggattaatta gtacttttac agctgaagaa gtacaagaaa ttactgataa tatgttggag
     8581 atagcaagaa aaatggaagt tccagagtat gtagaccctt ttattacact ttcttttatg
     8641 gcacttccag ttgttccaca aataagactt acagatttag gtctatttga tgttgaggaa
     8701 tttaaattta tatagatagt ataaaagtaa taataaatga ggctttctca aaatagagat
     8761 aagtctcttt ttattgttga aatgatgaaa atatatttga tttcatacaa aaaagagcat
     8821 ctcatgaact aaaaatttta tttttagata tccttatttt aattaatcta tcatttaac
//
LOCUS       DAELTA010000152         8766 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.152, whole genome shotgun sequence.
ACCESSION   DAELTA010000152 DAELTA010000000
VERSION     DAELTA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.152"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            119..886
                     /locus_tag="KPG13_002978"
     CDS             119..886
                     /locus_tag="KPG13_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBG0986676.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1104..1317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1571..2605
                     /locus_tag="KPG13_002979"
     CDS             1571..2605
                     /locus_tag="KPG13_002979"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG0986677.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2646..3863
                     /gene="argJ"
                     /locus_tag="KPG13_002980"
     CDS             2646..3863
                     /gene="argJ"
                     /locus_tag="KPG13_002980"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG0986678.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3878..4738
                     /gene="argB"
                     /locus_tag="KPG13_002981"
     CDS             3878..4738
                     /gene="argB"
                     /locus_tag="KPG13_002981"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG0986679.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4768..5955
                     /locus_tag="KPG13_002982"
     CDS             4768..5955
                     /locus_tag="KPG13_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG0986680.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6064..7086
                     /gene="argF"
                     /locus_tag="KPG13_002983"
     CDS             6064..7086
                     /gene="argF"
                     /locus_tag="KPG13_002983"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG0986681.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7213..7830
                     /locus_tag="KPG13_002984"
     CDS             7213..7830
                     /locus_tag="KPG13_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG0986682.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8050..8748)
                     /locus_tag="KPG13_002985"
     CDS             complement(8050..8748)
                     /locus_tag="KPG13_002985"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG0986683.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3523 a          855 c         1580 g         2808 t
ORIGIN      
        1 aataatatta tgtaaaataa aaatatatat gacatgatgt tgttgtattc aaaatattaa
       61 gataaaaata tatagaatat atttaaaaat tattatgcaa ttagaaaagg agataagtat
      121 gcagattatt gatataaata taaataatat tgacaatgaa catatatgtt gttcaatttc
      181 tgaaaaaaaa ggagaaaatt gtgttttttc taaaaaagct tggatgaaaa ataattttga
      241 taaaggtcta gtctttaaac gatttgatgt aagaggtaaa gcttttatag aatatattcc
      301 agcagaaaat gcatggtgtc ctattactgc ggacaattat atatttatta actgtttttg
      361 gatatctgga aaatttaagg gtcaaggata ttctaatcta ttgctacaag agtgtgttga
      421 tgatgcaaaa tcaaaaggta aatatggatt agttgtgctt tcgtctagta aaaaaatgcc
      481 ttttttatct gatgctaaat atctaaaata taaggacttt tgtttagcag atacatcaga
      541 accaaattat gagctattat attttccttt tactgataat gctcctattc catattttaa
      601 gaattgtgta aaaaaaagaa aattaaatga gaacggtata gttttatatt acagtaatca
      661 gtgtccacat acggaaaaat atgctcctat tttgaaaaaa attgcaaatt ctagaggtat
      721 tgatgtaaat cttataaaat tcgaaacagc aaaagaagca caaaatgctc ctgcaccatt
      781 tactacatac agtctttttt ttaatggtga atttgtaacc aacgagatac tttctgagaa
      841 aaagattgag aaatttttag ataccaacat taaaaaattt aactaaaaag agatgtaaat
      901 attgtatttt gaaaatatat tattttataa tttaatatat tttggatgtt ttttaaattg
      961 acgaatgaaa tataaaaatt atatcgcaat ttagatacaa aatgctggaa attaaccata
     1021 tagaaaatat ttccaaaaat ttaaaaatta tattgacaaa gttaaaaaaa taaactatca
     1081 tataaaacaa taagaaaata aattgaaatc ctgtgaaaaa gagagtagca taaagctagt
     1141 tgatagagag ctgggaatgg tggaagccta gtaacgaatt ttattgtgaa tagagctttg
     1201 gaggagatta ccagaagtaa tagtatggta attggtgaat ctcgccttaa gagattagag
     1261 tgggtaagat tatcttatca attagagtgg taacgcgggt gtatattcgt ctcttggtct
     1321 ttgaccaaga ggctttttta attttaaatg tacattttgt agatactttt taatttgagt
     1381 tttaatgatt attgtaattt taccaataag agtggtaccg cggaaaatcg cctcttagta
     1441 tttataatgc taaggggttt tttgttttaa ttagagtatt ttaatctacg tattatcaaa
     1501 tgattatcat atagtaaagt ggaaagatag gattttaaga ctatttgaat ataaaaatgg
     1561 agggaacaaa atgaataaaa taaaagttgg tattattggt gcaactggat atgtcggggc
     1621 agagttgatt agattattaa tgaatcatga taaagtcgaa gtgacagcga taggttcaaa
     1681 ttcgtatgtt ggaaaagata tagttgatat atatccaagt ataggatata aaaataacat
     1741 gatatgtata gaaaatgaaa aagttataga tatgtgtgat gttgtattta cagcccttcc
     1801 acatggagtt agtgaaaagt ttgtaataaa ggcaattaaa tcgaaaaaga aagttataga
     1861 tttaggtgct gactttagaa taaaagatga agaagtatat agtaaatggt atggagtatc
     1921 ttttatagat aaaatcttac ataaaaaagc tgtatatgga ttaagtgaaa tatacaaaga
     1981 agatataaaa gatgcagata taattgcaaa tcctggatgt tatccaacta gtatttcttt
     2041 accattgatg ccattactaa gttctaaatt gataaaaaat aataatataa taattgattc
     2101 taaatcaggt cttacaggtg ctggtagaga attatcgatt agtagtcact ttacggaggt
     2161 taatgaaaat atcactgcat ataaaatagg gaagcacaga catactccag aaatagaaca
     2221 aaatttaagt gaatcctgca aagaaaaagt aagtgtagtt tttacaccaa acttaatccc
     2281 agtaaatagg ggaatcttat caactatata ttgtacaaaa gaagataata tttctataaa
     2341 tgatatacat aggaaattaa ctgattatta tgaatttaaa gaatttatag aaattcttcc
     2401 attagataag gtagcttctt taaagaatgt tagattctca aataaatgtg taatatcatt
     2461 acatgaaaat ggagatacat tgataatctg ttcagctata gataatatga ttaagggagc
     2521 agcaggtcaa gctatacaaa acatgaatat aatatttggt attgaagaaa atacaggttt
     2581 aaaaaatatt gcaccatcat tttagaaata aaaaatacta aactcatata attaggagga
     2641 taaatatgga gattataaag ggtggagtta cagtttcaga aggatttttt gcttcaggga
     2701 ttcactgtgg cttgagaaaa aataaagaaa aaagagattt agctttggtt tattcagatg
     2761 ttttatgtga tgcagcagca gtagtaactc aaaataaagt aaagggcaat cctgtttatg
     2821 ttacacagga acacattaaa aatggaaaag cacaagctat catagtaaat agtgctaatg
     2881 ctaatacctt taatggaaaa gaaggactta taaatgctta taaaatggct aaattcacca
     2941 gtgataaatt aaatattaaa gaaagtgatg ttttggttgc atctacaggt gttataggaa
     3001 agccattaaa tatagattta atagaagaaa atatggatga attagtgaat aatctaagta
     3061 aacaaggtca tattggagca agagaagcta ttatgacaac tgatattata aaaaaagaaa
     3121 tagcagtagc aataatgtat ggagataaaa aaataactat tggaggtatg gcaaaaggtt
     3181 ctggcatgat acatcctaat atggcaacaa cgttaggatt tataactaca gatgcaaata
     3241 ttgatggagt tttgctaaaa gaagctttaa aaattgctgt tgacaaaagt tttaatagag
     3301 ttagtgtaga tggagataca agtactaatg atatggtaat gattctagca aatggaaaag
     3361 caaaaaatga tagaataaac aaaaaagatg aacattatca agtatttttg agtgcactta
     3421 catatgtctg tatagaactt gctaaacttg ttgcaaaaga tggagaagga tgtacaaaac
     3481 taattgaatg ctatataaat ggagcattaa gtgaagaaca tgcagtaaaa ttagcaaaaa
     3541 cagttatatc ttcttcatta gtaaagacag ctgtatttgg agctgacgca aattggggta
     3601 gaatactgtg tgctttaggt tatgcaggtg aagaaataga tatggaaaaa gttgatatta
     3661 tttttgagag tatgaaagga tatattgaag tatgtaaaaa tggaaatgga ttagatttta
     3721 atgaggaaaa agcaaaaaaa atccttgaag atgacgaaat aagtatatta gtagatttaa
     3781 atatgggcaa tgccagagga aatgcatggg gatgtgattt aagctatgat tatgtaagaa
     3841 taaatggaag ttatagaaat taggaggata tactttaatg attaatatag aaaaagcaaa
     3901 tacacttata gaagctttac catatataga aaaacatcag ggaaagacta ttgtagttaa
     3961 gtatggtgga agtgcaatga aaaaagatgg tctaaaagaa tctgttatgg aagaccttgt
     4021 gttaatgagt tatgttggaa tcaatatagt tttagtacat ggtggagggg cagaaatcaa
     4081 taagatgcta gcaaaagtgg atatagaaag taaatttgta aatggtctta gatatacaga
     4141 tgaagagacc atggaaattg taaaaatggt acttgctgga aaggttaata aagacttagt
     4201 aaataaaata catacaaaag gtggaaaagc agtagggctt tgtggaatag ataataatat
     4261 gattttatgt gacccatata aaaattatga acttggattt gtaggagaaa tcaaaaaagt
     4321 aaatgttgag cttatagaat cttgtctaaa gtcaggttat atttcagtaa ttgcaactat
     4381 aggtgttgga gatgatggag agacttataa tataaatggt gacacagcag cttctgctat
     4441 agctaaagag ttaaatgcag ataaattgat acttcttaca gatgtccctg gtttgttaag
     4501 agagccagat gaagaaaaat cattaataac agaagttata ttagaggatg ttgataaatt
     4561 atttgaagaa ggtataatta ctggaggtat gatacctaaa atagagggat gcgtagatgc
     4621 cttaaataac ggagtaaata gggtacatat tttggatgga agagttccac attctataat
     4681 aacagagcta tttactgata gtggaatagg tacattaata agaaaagaaa ctgaataaaa
     4741 ttataagttt aagaggggag aataagaatg aataacgaca atacaatttc aaaatggaat
     4801 gaatatttta tagatacata taaccaaccc aattttgtca tagattatgg tgaaggaagt
     4861 tgtttttttg atactaatgg caataaatat atagatttta caagtggata tggagtatct
     4921 tctcttgggt atagtaatag taatttaaag aatgctctaa aagagcaagt agataaatta
     4981 ttacatactt caaatttata ttttaatgag ccagtgttgt attcaggaga gaaaataatt
     5041 aattcaagtg gaatggctaa agtatatttt tgtaattctg gaacagaagc aaatgaaaca
     5101 gcattcaaaa tagcaagaaa gtatagtagt gacaaatatg gtaatggtag aggaactatc
     5161 ataagtttaa aagactcttt tcatggtaga acaatgatgt ctttaatggc aactggtatg
     5221 gacaaatacc ataagtattt ttatcctctt cccgaaggtt ttaaatatat agaaagaaat
     5281 aatattgaag atttaaaaaa taatcttgat tccacagttt gtgcaataat tcttgaagcg
     5341 attcaaggcg aaggtggagt taatgttctt gaaaaagatt atgttttgga aatagtaaaa
     5401 atatgtcaag aaaaggatat tgtagtgatt tttgatgaag tccaatgcgg tataggaaga
     5461 actggtaaac tttttggcta tgaatatttt gatgtaaaac cagatatagt taccgtagca
     5521 aaagggttgg gagcaggaat accagtagga ggagtattgg taaataaaaa actttcaaaa
     5581 gtactaggaa aaggagacca aggtacaact tttggaggta atttattagc tatggtggca
     5641 gcttcagttg tattagatga gatatctaaa gatggctttt ataatgaagt attagaaaaa
     5701 ggaaattata taagaaaatc tattgaaagt tttaataaca aagttatatt aaagacaaaa
     5761 ggtattggtc taatgatagg aattgaaact aatattgaat ctagtattat agaagagaaa
     5821 gctagaaaaa aaggattgct tattttgact gctggaaaaa atgtattgag atttttacca
     5881 ccattaacaa tcagttataa ggaaatagat gaggctcttg aaatcctaaa agatatatta
     5941 ctagaaatta actaaatatt caaaaaatag ttgattaaag ataaaaatta gaagtaaata
     6001 aaaaagtaaa gtgctaacaa gtttaattga atttaaaaag tttataagaa ggggcgaatt
     6061 aatatgaata atataagttt aaaaggtaag agttttttga cattaaagga ttttacaaaa
     6121 gaagagatta gatatttatt aactttatca agttatttaa aaactaagaa aaaagttggt
     6181 ataaaaggcg atttgctaca aggaaaaaat atagctttat tatttgagaa aacatcaaca
     6241 agaactagat gttcatttga agtagcagct catgatgaag gagcacatgt aacttatcta
     6301 ggtccaaatg attgtcatat gggtaaaaaa gagtctgtat cagatacagc aaaagttcta
     6361 ggaagattct atgatggaat agaatttaga ggctttaaac aagaaacagt tgaaagtcta
     6421 gcaaaatatt ctggtgtacc tgtttggaat ggacttacag atgaatatca tccaacacaa
     6481 atactggcag attttttaac agtaattgaa aatgtagata aagatttgaa taaagttaaa
     6541 tttgtatatg ttggtgatgc tagaaataat atggggaact cactgatgat aggatgtgct
     6601 aaaatgggaa tacatttcgt agcactagca cctaaggaac tttggccaaa tgaagaattg
     6661 gttaaggaaa tgaaggaaat agccaagaaa tccaaaggag atataatgtt gactgaaaat
     6721 attgatgatg ttaaaggtgc agatgttata tatacagatg tatgggtttc tatgggagaa
     6781 gaggagcaat atgaagcaag aataaatcaa cttaaagatt atcaagtaaa tatggatatg
     6841 ataaagaaaa ctgaaaatga aaatgttata tttttacact gtcttccagc atttcatgat
     6901 ttagaaacta aggttgctca ggagatttat gaaaagttcg gattatcaga attagaggtt
     6961 acaaacgagg tatttaattc aaaatattca aaggtatttg aagaagctga aaatagaatg
     7021 catacaataa aagctgttat ggtttctact atgactgatg taaatatgaa caataattta
     7081 atatagatat gttttaatat agactttact ttaatgaaaa tttataaaat attttaaata
     7141 tattattcaa aaactctcat tatatgttaa aataacttat gtatcaacaa attaagaggg
     7201 aggatttgag atatgaaaaa aatcatatta gctttaacaa cttttttatt agcaatatct
     7261 ttaacagcat gttcaagtgc aaaaccagaa gatacaattg atagtttttt taacagtgcg
     7321 aaaaagtttg actttgaagg catgaataag gtaatggaaa ataatgacga aaaatataag
     7381 gatatattaa aggaattaga tacaaaagac ccaaatgccc aatatgtttt agattatcta
     7441 aaacagaatg catctaaaat cacatataca ataaaagaca gcgaggttaa aggtgataag
     7501 gctacaatca aagtagaatg taaatttata gattcaacac cattacttca agaaatagta
     7561 gctgaggcat ttacaaagat gattggtatg agtttttcag gacaagactt aactgatgag
     7621 aaaacaactg aaatgttagt atcaattatg aaagaaaaac aaaaaagcgt taaagagact
     7681 tatgttacaa aaaatgtaga gtttgaatgt tctaaaaagg ataataaatg gataataagt
     7741 tcagtaaatg atgcagtagc tgatgtattg ttatctaatc ttgttacagc aggacaagaa
     7801 ttttctaact ctatgaatac acttgaataa tattgtttag gtgtagtaaa gtaataaata
     7861 tatgattatt tttataaata aacatataaa tatttatgaa ggataccatt tttggtatcc
     7921 tttttattaa tataatgaaa tacatggtta atttctatat tgtcaagaaa ctatgatata
     7981 tgttataagt ctatactatt taaaataaac ttataacata tattttttat aatatagtgt
     8041 atgaaaaatt tatttaacta attttagata tttattgaca actgattcag ccaagcattt
     8101 tgatgaatca acaatattta ttgaaggata tgatttttct aaaactacat ttaaatcagt
     8161 acaagcaatt actatatttt ctatagattc atttttaaca tcttcaataa gtttattcca
     8221 aatatcaata ttgtgtggat tttctttatc catttttata ttttgaataa gattgtttaa
     8281 ttttatttgc cattcatctt taaatataaa ttcatgtcca ttatgtatta taccattctg
     8341 gtaaattgta gactcaaaag ttgaactagt cgaaaaaaga gttacttttt gtgagatagt
     8401 aggtaatttt ttaactgttt cttctacaat atttaatatt ggtatattaa tagactcttt
     8461 taattcttta aagtatatat gtgctgaatt gcatggcatt gcaataaaat ttacaccagt
     8521 tgattcaagc ttttgtaatc catcaattat agtttctttc ataaggtcat gatttattgg
     8581 acggtcaata taaaatgggg taggtaatga atatatcatc atctttggaa attcatcatc
     8641 atattttgct ccatattgag attgacactc atcaataatt aaatcaacaa aaggggctgt
     8701 tgaccttgga cccattccag ccaaaatacc aattaatttt tcattcataa aaattatctc
     8761 ctttat
//
LOCUS       DAELTA010000153         8681 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.153, whole genome shotgun sequence.
ACCESSION   DAELTA010000153 DAELTA010000000
VERSION     DAELTA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.153"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(70..300)
                     /gene="dltC"
                     /locus_tag="KPG13_002986"
     CDS             complement(70..300)
                     /gene="dltC"
                     /locus_tag="KPG13_002986"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG0986684.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(319..1467)
                     /gene="dltB"
                     /locus_tag="KPG13_002987"
     CDS             complement(319..1467)
                     /gene="dltB"
                     /locus_tag="KPG13_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG0986685.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1467..2981)
                     /locus_tag="KPG13_002988"
     CDS             complement(1467..2981)
                     /locus_tag="KPG13_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG0986686.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3102..4292)
                     /gene="dltD"
                     /locus_tag="KPG13_002989"
     CDS             complement(3102..4292)
                     /gene="dltD"
                     /locus_tag="KPG13_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG0986687.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(4580..5599)
                     /locus_tag="KPG13_002990"
     CDS             complement(4580..5599)
                     /locus_tag="KPG13_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0986688.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(5775..6314)
                     /locus_tag="KPG13_002991"
     CDS             complement(5775..6314)
                     /locus_tag="KPG13_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG0986689.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(6415..7095)
                     /locus_tag="KPG13_002992"
     CDS             complement(6415..7095)
                     /locus_tag="KPG13_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG0986690.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(7124..8413)
                     /locus_tag="KPG13_002993"
     CDS             complement(7124..8413)
                     /locus_tag="KPG13_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG0986691.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         2866 a         1528 c          837 g         3450 t
ORIGIN      
        1 aatattagta tcagatggac tttgtaaaat tttattttat aaattaatat ttaaagtcta
       61 actactaatt tattttcttg tctccaagaa ttttactaaa ttatttacag tagacatatc
      121 ttttctctct aaatcagttg gttgaagctc tatccctaat ctattttcaa tttcaagtaa
      181 aacttcaatt atagctaatg agtctaaaag ttccgtctca aataaattaa ggtctaaatc
      241 ttctcttatc tcatcagtcc ctaatacatc ttcaaatatt tctataactg tttcttgcat
      301 aaattttcct ccttaaaatt aagctaaata acctgagaat atcagcaatc caaaacacac
      361 aatatggaat gtaatgaaaa tctgcactct ctcgaaccat ttatcttttt tatgtttttt
      421 ataaaattta gattttcgct gatatatatc agttaacaca agtgccaatc cttggtatac
      481 accataagca acataatacc aagttagacc atgccaaaat cccattgtta tcatggttat
      541 catctgagcc acatgagcag cagtagtcct tttcttaaat cttttctttc tcattgatga
      601 cataacaaat ctagaaaaaa tataatctcc aaaccatctt gaaagactta tatgccatct
      661 tgtccagaac tctttcatat ctttactgat aaatggcttg tcaaagttta taggtacttg
      721 tattccaaat atgtatcctg tacctatagc aaataaacta tatcctgcaa aatcaaagaa
      781 caggtataaa ctatatgcgt acatataact taaaatattt ataaaactca tatctttagg
      841 tattcttgat acccaatatg tatttatcaa aaatgctata acaaacttat aacctactcc
      901 catgacaata ttttttattc ctggcagtaa gtattcttct atatactctt ttctagagat
      961 ttgtttttcc aagtcctgtt caaatcttct tgacctatca ataggtccag aacttaaagt
     1021 tggaaaaaat aacataaaat aaagcatctt tgaaatttta acttctttta tagctccatc
     1081 ataaatttca ataaccattt gtatagttct gaaatttaag tatgatattc caataaatcc
     1141 aattattcca aaagaagtaa ctggagatat tttatttatt attattggga gcattgatgc
     1201 aaacaagaat ccccaatata tgtatttatt tttagttttt cttcttacat attcatatcc
     1261 tttaactatt attacttcaa gaataataaa tattaccaag tactttagtt ggacatcaat
     1321 tcctactatt aagaatatca taaacaaact tgccaacata ccataatact ttatattctt
     1381 gcccattaga cctaaaatta ctgctggtat tgatgttaat aataatatat aaagataaaa
     1441 ataatctcca tattgtgaaa aaatcattaa atttcctcca taagtttctt tctgtctatt
     1501 ttaccattga tatttgttgg aaactcagag attattgaaa tatttcttgg aatcatatat
     1561 gaaggtatat actttccaag ttcctttttt attatcatcc catttttaat attacttaaa
     1621 tcattctttt catttaactc aacaatacct tttaagtaag caattttttc gtccttatat
     1681 actggtaaaa ctactgcatt ttttacatta tgtacttttc tcaaattatt ttctatatct
     1741 tctatttcaa ttctaaatcc atttaattta atctgaaaat cttttcttcc acaataatat
     1801 atatttccat ctaaaagata tcccatatct ccagttttat aagctctcca ctttacacca
     1861 tctatttcat catagaaaaa tacttcatca gtcttttctt tattattgaa gtaaccttta
     1921 gaaactgatg gtccaattat tataatttca cccttttcat tttctttaag ctcatttcca
     1981 tcttcatcaa gtatttttat tttacaattt gacatagggt atccaactgg aagacttttt
     2041 tcatcatcta tagccttttg agtcatatca tttacactta caccaacagt agcctcggtt
     2101 ggtccatatc cattgataat tcttgtgttt ggaaatctac tcataagttc tttagtaaga
     2161 ttttttgaaa gtgcttctcc tatgaatatc attgactcaa ggttaggaag cattttacta
     2221 ttaaactctt tttcagttat acatactcct gcaaaagaag gagttgacac ccatacagct
     2281 atatctgaga tactaaattg tctaaatagt tctttatagt ctgctaatac atcttttgat
     2341 attgaaaata atgtagctcc atggataaga ccagggtata ttgttgtaac tgaaaggtca
     2401 aaagaatatg ctggttggtt cattattact ttttcacttc catctatgtt tagatatggg
     2461 cttatccaat ctgaaaagct atctaaattg tttgaactta tttgaacccc ttttggcttt
     2521 cctgtacttc ctgaagtaaa tagtatatat gcattttcat catcttttac ccagttttct
     2581 ttatctaatg attttccctg atattcattt attatataat ttaatttatc catatcaatt
     2641 acattgatat ctccaaaatc tttttcatca ctaaagttaa ataatacctt tggttttatc
     2701 tctttagtga cttcaaaaac tctatcaatt ggaaaactaa tatcaagagg cacatacgct
     2761 ctacctgatt ttaaagctcc tatcatacat gccattatca tgttttcttt atttccataa
     2821 attactatag gagtatcttc ttctttgtat acatccttta gaaatactga tatagcatct
     2881 gaatattcat ttaaatcttt ataagaaagt ttatctccat tacacattag tgctgttcta
     2941 tctgtatttg aatatttttt tattccttca ataattttca tataagaatg ctcctttaaa
     3001 atccgttata aataaatggc aattctttaa atggagtaaa tgttagaact cctatcgcca
     3061 atattataac catagttaaa caaatactag ttaacaaatc tttattgttc tttaaatatt
     3121 ttaaataatc tctcacagac atctacccat cctttcgtac caagatgcat tacatccctt
     3181 aaataatact tatctgaccc tttatcttta aggtttagaa cttcaaagcc tttgctttct
     3241 gctatttttt gtgctttatc gtagtactga tttctttctt tttcagaaat accagtcaaa
     3301 ttatagaatt tatccatact tgggattaat acaacaactg gcttaatacc taagtcagta
     3361 catacattta gcatcagttt ataattttca aactccttag atgttaataa atttacatct
     3421 ttgtctcttc ctttgtattg ctctatacca tctttaaaat gctctttata atagtactta
     3481 tcaatattta gtggattttt accaactctt tttttagcat cttcaatggc tttctttctc
     3541 tcatatgacc aatttattgg tctttttctt tttgttgctc tctttttatc tagcttaatt
     3601 aatcgtttat aaagaatacc cttttcttta agagctatac aatactttct tccttgaaaa
     3661 taaggctcta aaagcacctt ttcagctttt tcaggaaatg tcttaggctc atatagctta
     3721 gcatataatg cttctgcttt atactcgtca cttccccata ataacttact tgattttttt
     3781 gcatattcaa tcttattttg tttagatatt tttggattat ctaaaaatcg ataaaattgt
     3841 attggtgaaa atctagactg ataatggtga cttgtgactc cgtctttttc cataaaccat
     3901 tgcatagaaa ttaacaagac aactttctta ttatcaatat taggattcat acttcctaat
     3961 attgcagcat cttgtaatgt ctgagtatag gctctcccaa ttgcaaacac cttattttta
     4021 ctcctatttg tattaaaata gtactttgga tgttgttttg ttgaattact aagttctgat
     4081 gaccccaaaa tcataatatc cttttctctt aaaaaatgat tatttgctgt aaccccttta
     4141 tcttttatat cacttaaagt atcatccata attggaagta gatttttctc cctcaaaagt
     4201 tcatcagttt tagaatcaag aaatttgtct aacccaaata aaaatactac gcctattata
     4261 aaaggtgtaa taaagtatat caattttctc atattttctc tcctctaaaa atattcaaat
     4321 ttatgttatt attaatcata ttagttttga tatgtatatt caactacaaa attgtaataa
     4381 aatcgtcaaa actttttttg atactgttac aaaattgtaa aaaaaaagaa aaaaacgcca
     4441 aaaataaatt tctttttagc actttttcat acttataatt tgacatttta acacttggtt
     4501 aagtatacac cttaccttaa cttaaaatta aatttattta agaatatata ttataaattc
     4561 ttatattttc taatataaac tatatactat cagctggata aacaatacta ctttgtcttc
     4621 ttatctcatc cataatttcc attactatta ttgaattttc atgagaattt atatttgatt
     4681 ctattttatt gttatttata agacttataa actctacaag ttcataatac attccttcta
     4741 tttctctagg cttttcctct gctttttctt ttggaataga tatatcttct tctcttccat
     4801 ctctgtattt aataatagct ttttcaaata gattaagctt ttcaattatt atacttccct
     4861 cttctccttg tatttcagat ggtatataag aatcagctac tttagaatac tgaattatcg
     4921 catccatatc atcatattga aatacagctg aaccttctcc atccactcca gtttgaagta
     4981 tatgagaaac tgcctttaca gtttttggag caccaaataa attcaccata ggatgtatac
     5041 aatacactcc tatatccatt aaagctccat ttgacaactc ttttttaaag gcattttcta
     5101 taattccatt tttatattta tcatatcttg aagaatactg acaatagctt ccaaaaaatc
     5161 ttcttatttt ccctatttta tataagtttt ctttaactgc tttaaagttt ggaacacatg
     5221 taattctcat agcttccata agcaatacat tattatcttt ggctgttttt atcattgatt
     5281 tgacttcttt taaattagat gctaacgact tttcacaaag cacatgtttt ttgtgtttta
     5341 aacaagtaat ggtttgcttt gaatgtaatg catttggaga agctatgtat acagcatcta
     5401 tatccttcga tttagccatt tgttctaaat catcaaaaaa aatacttgct ccatatagag
     5461 ttccaaattc tctagctttt tctaaatttc tagaatatac agcaaccaat tcaaagtctt
     5521 taaccctaga tgcagctctt agaaatatat ttgctatatt gcttgtccca acaataccaa
     5581 atcttatttt acccatcata aatcactccc cattttctac actttatttt aattttaata
     5641 aaaaacttta caacttatag atatattata aaataatttt atcaaataaa aaagaaagct
     5701 aattttaaat gatttcacca ttttaacata gctttctttt aactacaatc taaattcaac
     5761 ttatattttt aaagttatct ttctactata tctttatttc ccctcaaaaa ttttatagta
     5821 agaatttcat ccacaaacaa tgttactaaa agtatagtta caacaattgg ccaataaggt
     5881 attataagac tatataaact atcaactata tacacttgaa aatatctaac tccaataaat
     5941 gtcaatatag tccaatatac agaaaacttt agacatacta ttcctctact attgtactta
     6001 taatctgaat aatcccaata gaccttatta aaatatactt tcataagtat tcctgtaata
     6061 tattctacta ctgtcggcac tactagacat aataaaagca atgcaaatat attattgggg
     6121 tcaataaatc ttgagacctc tattataatc gacattgcaa ttgcatacat tggtttaaat
     6181 ggaccattta aaaatccatc ttcttgaaaa tgcccttgtt tataataact atataagttt
     6241 tcaattatcc aacctataaa accatacaag aaaaaattaa ataaaaaata taatagagaa
     6301 cctacattat ccatgaatat tcctcctagc tgtttatact tattttttct catattcatg
     6361 gatttattct aaatcaaaga aattatattt aatttattta attaatactt tatattatag
     6421 attgtgacat tctttagagt tcaaactttt taggtattct ctaacttcca gtaaattttc
     6481 aagtatctta tgtgattgtg atttaatttg ctcatctggc tcttttaaat ctggtacatt
     6541 tatacatctt ataccaccat tgtatgcagc ttcaactccc ataggcgaat cctctatgac
     6601 aatacaattt tggggattaa catttatctt tttagctgct tttaagaata tctcagggtt
     6661 tggtttagag tttacaacat catctccaca aactatagca tcaaagtaat cttttaaatt
     6721 tgccttagct aatctcttta ctgctctttc tctctttgtt gatgttgcaa cagccatttt
     6781 gtatccattt tcctttaaaa aagagattag ttcattgact cctaatttga taggagctcc
     6841 cttcctctcc ataaattcaa tcatattttt agttttttcg tcatataaat ctattattgg
     6901 tacagaacta tcgtatatat ctgtaagacc ttctattatt cctttacgat ttcttcccat
     6961 aactgatgta taaatttcct tagtcatagt atatccatac ttttcaaacg tctccatcca
     7021 aaattcaaga gaaattctct ctgagtcaaa caaaactcca tccatgtcaa aaataattcc
     7081 ttctactttt tgcatacaat tcctccaata ttttttgata taattatatt acacttttta
     7141 tgattaattt tacaaccctc aatttatcca attctaatat tgtaaaattg taattttcaa
     7201 aagttacact ttggtttact ttaatatttg aaccaagcat ataggatatc cacccaccta
     7261 tactgtcaaa tccatcatgc tctatatcaa gtccaaaata cttattaata tagttgatag
     7321 attcagttcc atcaactaag taagaacctg ctcctatttt cttaatatga gtctcttctt
     7381 tgtcaaattc atcttgaatt tctccaacta tctcttctag tatatcttct attgttacca
     7441 caccagatgt accaccatat tcatccacaa ctatagcaag ttgaagtttt tcctttctta
     7501 ttctctcaag agctttatct atagttaaat tttcagatat atatataata tctctcaata
     7561 tttcttctag ttcaatttta ttttcattta tcttttgatt gtataaatct cttatatgta
     7621 caaaacctaa tatatcatct ttattttttc tgcatactgg atatcttgta acaccttcct
     7681 cttttaaaat agctaatatc ttttcttcag aatcactttc atatatacat accatatcag
     7741 ttctaggaac cattatctct ctaattttct tttcttcaaa ttcaaaaata ttatcaacta
     7801 atctttgctc tgattcatct ataagtccac ttttataact ttcttctact aatagtctta
     7861 tttcgtcacc tgtatggggt tcatcaacct catcagcttg tgaatatcca aatggtttaa
     7921 gaaataaatc agtacttaag ttgaaaaaat atattattgg atatgttaac ttataaaatc
     7981 ctactaataa aaaagatgta ctcaacatta ttttttctgt attataaagt gctaaagctt
     8041 taggaaccaa ctctcctatt acaacctcca tcattgttat aattaaaaaa gaaatggaaa
     8101 ttgatatagt atatataata ctttcactaa gattaaatac acttactatt ggtaaaatta
     8161 actctttaac tgtagattct cccatccaac ctaatgcaag tgaacaaagt gttataccaa
     8221 gttgacatgc agaaagatag gaatttaagt tgtcttttat tattttacat ctttttgcat
     8281 tattattacc ttcagttatc atggtatcaa tcctagaact acgaagtttt accatagcaa
     8341 attcacaagc tacaaaaaaa gcatttatca aaacaagcat tactataagt gttactttta
     8401 ataactcccg cataaagtat tggttcttat aatgtaagaa atctataaga actcaaagtt
     8461 ccccccttat attaataaat ttaatttata atatatattc tatcatattt attaatatat
     8521 cctactttat tacaaataat ttttataaac taaataaccc cttttaatat ttatgactgg
     8581 ctatttgata aatattaaag gggttattta gttatcattt tttatttcta aaaatctttt
     8641 ctcaaatgat ttagttatct atttatccaa ttttttacat a
//
LOCUS       DAELTA010000154         8631 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.154, whole genome shotgun sequence.
ACCESSION   DAELTA010000154 DAELTA010000000
VERSION     DAELTA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.154"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(163..963)
                     /locus_tag="KPG13_002994"
     CDS             complement(163..963)
                     /locus_tag="KPG13_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG0986692.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(1277..2008)
                     /locus_tag="KPG13_002995"
     CDS             complement(1277..2008)
                     /locus_tag="KPG13_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986693.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(1995..2690)
                     /locus_tag="KPG13_002996"
     CDS             complement(1995..2690)
                     /locus_tag="KPG13_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0986694.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(2692..3483)
                     /locus_tag="KPG13_002997"
     CDS             complement(2692..3483)
                     /locus_tag="KPG13_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986695.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(3493..4305)
                     /gene="modA"
                     /locus_tag="KPG13_002998"
     CDS             complement(3493..4305)
                     /gene="modA"
                     /locus_tag="KPG13_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0986696.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(4939..5139)
                     /locus_tag="KPG13_002999"
     CDS             complement(4939..5139)
                     /locus_tag="KPG13_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0986697.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(5364..5540)
                     /locus_tag="KPG13_003000"
     CDS             complement(5364..5540)
                     /locus_tag="KPG13_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986698.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(5594..5683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5863..6063)
                     /locus_tag="KPG13_003001"
     CDS             complement(5863..6063)
                     /locus_tag="KPG13_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986699.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(6065..6415)
                     /locus_tag="KPG13_003002"
     CDS             complement(6065..6415)
                     /locus_tag="KPG13_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG0986700.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            6577..6765
                     /locus_tag="KPG13_003003"
     CDS             6577..6765
                     /locus_tag="KPG13_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0986701.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(7242..7412)
                     /locus_tag="KPG13_003004"
     CDS             complement(7242..7412)
                     /locus_tag="KPG13_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986702.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(7801..8289)
                     /locus_tag="KPG13_003005"
     CDS             complement(7801..8289)
                     /locus_tag="KPG13_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0986703.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
BASE COUNT         3140 a         1162 c          997 g         3332 t
ORIGIN      
        1 ttataggttt taatgatgat atctttatat attttaagag atgatgaaat tagatatggg
       61 taatagttgt atgagaaaaa gtagtatagc acaaaaagtg ctatatcaca aaatctcaaa
      121 taatttcctt tttataactt aaattaaaag aataaatatt tcttataaat taggtgtatg
      181 aagtatagca ggaacatctt tattatttaa taacttatca aatgatttat ggtcagactt
      241 atagccaata ccttctctat attctaattc atcaacaatt ttgtattgtt gacaggcatt
      301 tttaaccttt tcatagtctt cagtagaatg agctttataa actatcttat tattaaaagt
      361 tattttatca gcaaatttag gttttacaaa atatttagca tcttttattt taccagcatt
      421 atataaattt tggataacag aaccattttt taaatgttgt aaatggtcat ctaacttata
      481 agtatgtgct atatgagtta catttttaaa tttttcttca aatgtaagag caattttttc
      541 catgtaattg aagtcagtat taccatttgt atttgtttct ggtaataata ttatattttc
      601 ccttattaca ccaaggctat taagagaagc taaaaattct ctatttctat attctgattt
      661 ttgtatattt gttaggtttt catatttttt atatttatca aagacggata taccacatcc
      721 agtagatact aaaaccacaa atacatgatt ttttcctctt tcttttatag cattaacaat
      781 agcactccca gcccacaaca cttcatcatc ttgatgctga acatagaaga caacataatc
      841 tttaaatttt ttatgactat aattcacata gctactaaaa gcggttaaaa tcaaacataa
      901 aactagaata atagatagaa ttttatattt atttatagta aattttttat tcttaaatgt
      961 catttaatca tctcctgaaa agttgtctta gttatattat caaacaaagt actaaaaatt
     1021 atttcttaaa tgctgaaaaa tatagagtag attaatggaa taaaattttt ttattagttt
     1081 aaaataaaga aaacatttat gtataagact aatcaaaata ttaatgattg ataggaattg
     1141 agatttaaag ggattgtaca tttattcggg attcatttta atagaatttt actttagtac
     1201 ttagaataaa agtaataact ttttaaattt aaagagtaca catatatatt tgtgtactcc
     1261 ttgatgatat gattgcttat ttactacaaa aacagagtct ttttaaacct aaaatatttg
     1321 atgcaccagc tatcgtagtt ccataatagt aaactggttt gttaagtgat agaaaattta
     1381 taattgagtt attacataat gtacttcctg tgcaaatcac taaatcagcc caagatatta
     1441 catcttcata atctcgtata ccatcttcaa tcttaacatt atattggata gtatccacaa
     1501 attcaggatt taaatctaat actcttgttt cgaaaaagtc cttaatatta tcaattatgg
     1561 ctggttgata tcctataatt gctacttttg gattaccaaa ttccattttt ataaactcag
     1621 ggaatttttt tgcacatatc tctggctcgt tatttttaca atgaatggtt ctatccgtct
     1681 tgcctaagta tttcataact gcatttaatg atgctataaa aagacttcta tcatgaggat
     1741 tgtcatgtaa atctaatgat aatatgtctt ttaaagaacc ttcaaatgta gatggagtag
     1801 aagtaaatgc ttgtccttta gctcctttaa agtcagcttc aagcattatt tctttaccaa
     1861 ctataattgg gaaatcttta cgcttagtat ttccaattgc ttcttttgaa gatagagatt
     1921 tggacacaat acttatcata tccatattta aattatattt atcaacttgt actaatagat
     1981 aatctctcaa aatatcaaat atattcatag aaaaaagatt ctcctttatt ttttatttct
     2041 tcatttaaat caaaaatatg atttattttt ccattgttca ttattcctat cttatgagca
     2101 aaaaacattg cttcagaaat atcatgagta acatgaatca ctgttggttt gtattttatg
     2161 tagacatcct tcaaaatttt gcttatatgt ttttttgtaa tataatccaa tgaactcata
     2221 ggttcatcta aaagtaatat atcaggttca gctattaagg cacgtgcaaa agcaactcgt
     2281 tgcttttcac ctccacttaa atttttagga tacctatttt ttatatcttc tatctgcatt
     2341 aatttagata agtggttagc cttaagaatc tgttcatcta taggtatttt tctcaattta
     2401 agtggaaacc ttatattatc ttcaacattc atatttggaa ataattcaaa attttgatat
     2461 acaaatccta tatttctatt ttctggaggt aagtttgata ttaaattttc tttgtatgat
     2521 acatttcctt tagcagatgg atatcttcct gctaatgttt ctagaaatag agttttacct
     2581 gacccactag ctcctaataa aacaaagtat tcatgattat taatatttaa actattttct
     2641 tgtaatttaa aattaggtaa atcaagagat aaatttttta tttttaacat attaattctt
     2701 atcaacctct ctattaaaaa atatctcaaa aagcatcaag catgtaagtg agataaatat
     2761 caaaattaca gctattccga ttgcaatgtc tatatcacca gtagacatat ttaaatatat
     2821 agatgtagga ataatttcag ttttcatacg tgtagaacct gctatcatag ctactgcacc
     2881 aaattcacca agtgctcttg accaagtcat tacagttgca gatatgattc cagtttttag
     2941 atttggaaga attataaatc taaatgcttc ccaactatta catccaaggg tacgtgcaac
     3001 aaatatcatc ttttcattac tttcatttag tgaagtgtaa agtattttaa tagtaagtgg
     3061 aagatttaca aatacttgtg ctaaaactat accttgcttt gtaaatataa aatcaagctt
     3121 aaaaatctta taaatggaat ctccaatccc cattcttcca aacaaaagta aaagagcaat
     3181 tcctgatact aagtgtggaa gagacatagg gagatttatt atctgtataa tcagtttttt
     3241 aaatggtaat ttagtaatat gtaaaaaata tgctactgga atagctaata aaaggcatac
     3301 tatagtagat attaaagatg ttttaatact aagtattatt gcatactgta cttccactga
     3361 ttttaagcat ataggaacat aacttatccc tttaatgatt aacataagaa gtggtgatac
     3421 aataaacaaa aagactatag caaaaaatcc agtaagtaga gtagtaaaaa atttattttt
     3481 caagtttaat cactacttaa caggttcaaa accatatttt ttaaatatct gtttagcttc
     3541 gtctgactct atataattta taaatttatt agctaaatct tttttatcac tatagtttac
     3601 gattcctgct gatataagtt ggtctatatt tttatcttgg gcaatttcta taacttctac
     3661 atcttcattg ccaaagaccg aatcttttgt ggctattgta gcatcagctt tacttgatgt
     3721 taactgtaca atcatttcat ttacagttgc agctgtagag acaacattat catttataga
     3781 ttttaaatta tttttttcaa gtattttttg tgtagtctta cctatagcat tagactcttt
     3841 gtctcccaaa ataagtttaa ctccatcttt atttaagtct tccaattttt gaatattagc
     3901 tggatttcct ttaggtacaa ctattgcagg tgtatgatgt gcaaccgttt tatgttcacc
     3961 tacaagttct ttttcaaagg cttttgaata aattggttca gaaccaacaa taaatacgtc
     4021 ccctttgtga cttgtctcaa tttgagctat tagctgtgca gaacctgcat agctatattc
     4081 aactttgaca ttattttctt tctcaaattt ttttgctatt tcatccattg gtttctttaa
     4141 acctgcccct gaatagacaa aaagagtctc tttttctttt gaatcatcag ctgttgtcac
     4201 tttagtatta gattcatcat tagctttgtt ttctttagaa ttacatgcag taatacttaa
     4261 tgaaataatc atagctaaag ctagtactga aagtttttta aacataagat attccccctt
     4321 agtaaattaa tgtaaattct tttttagtat atcatactaa gaaaacgatg tctaagtttt
     4381 tgttatgttt tataatgttt tgttatgttt tgttatgtat agttaagaaa aaatctattt
     4441 ataatgttat ttctataggt agttatttta tttttaattt ttattaatac tttaaagtat
     4501 tttaaattta aaaacttaaa tcaaattaac ttattgtaga ttttttactt tttaactaat
     4561 tttatattta ttatatttca gtgaaaaaat agaacattga aacatataga tatgttttaa
     4621 ttgcttttta ttaacaaatt actaaaaatg ttatatcaga attgaataca aaagaaagag
     4681 tatgtattta tgagatagtt gaaaattttg atgtgccaca actaaaatta ttatattact
     4741 atatatttaa ttaaaagagt acttgtaggt ttaagtacat actttataaa tatatttttg
     4801 ttagatttat tttataaaaa gtatttagac taagtattat ttttaagtat tttcttgttg
     4861 aagttatttt ttcctttttt acaaaattcg taaataaaaa aaagactctt aaaagagtct
     4921 ttccgaaaaa tctatatatt ataatatagt tatattttct gcttgaggac ctctagcgcc
     4981 tttaactata tcaaagctta ctttttgacc ttcttctaat gatttaaatc ctgaagtttg
     5041 tatagctgag aaatgagcaa atacgtcatc tccactttcc attgatataa atccaaatcc
     5101 tttttcatta ttaaaccatt ttactattcc attattcatt aaaaatactc ctattaacct
     5161 atatcaagat atacttgatt tacttgtgta ttaccacaaa tattattgta tcatagatta
     5221 acataaatgt ctatttaatt ataattttta gttaacattt ttatttattc ttaatatgaa
     5281 aataaattta aacattttat attaccaata tattaatgta aatgtagata aaactaaaaa
     5341 gggttttcta gaaaaaaatc tgtctaatta tacttactta ccaataccat cacttgatgc
     5401 aagcaagatt ccacaacttt ttctccgtac tctaatataa aatcatctaa ctgtgtatct
     5461 ataaaatcac aataatcgta tgaattacta atataagcag ttaacatatg tgtacattta
     5521 tccattttat ctctaaacat aatgtattta tccccctaaa aaactttcat gaaaaacaaa
     5581 aagaaaatat ctttttggca actggctgac ataacttatt ttataagcgt tagtccctat
     5641 agctttgcgt cactaaattt ctctagtttt gccgatttag ttttattcta cagattaata
     5701 atacaataga attagttatc attcaacata tctagttgaa taattaataa atttatatat
     5761 gaactttaaa ttatttgaaa aaataaaaag cccctctgtc aggagtgact taaaaattga
     5821 atttaagttt gtatttaaaa cacattttta ataatgcaaa aactaatcaa aaataaacaa
     5881 ttcatcaata ggtgtatcta ctatttttga tatatctatt gctaatttca aagaaggatt
     5941 atactttcct gcttctaaac gcataatggt ttctcttcta actcctactt tttctgctaa
     6001 ttgttcttga gttaattctt ttaattgtct atattttttt aggttacaag tgaatttagg
     6061 catattattt ttcctctaaa atataaagtt ttatagcata aacatttaaa gtgattgcat
     6121 atgtaattga tagtaaaaca cacattaatt ctatattcct agacactata gtaatacacc
     6181 ataaaatgaa taattctata atagcaagct gtcctgtaaa tgccatagct gctttttcat
     6241 cttgtacata cctttcatct gctttatcac catttatttt tgctataata aagtttgaga
     6301 aaaaagcaaa aaatccaata tatgtaaggt cagcaacatt gccagatgaa aaatatctaa
     6361 gacttaaaaa tccaaggaaa ccacaaaaac ccataaaaaa ttttttctga atcatttttt
     6421 atcctcctta gtaattttaa aagagatata tttatatctt tatgatacaa atatatctct
     6481 tttatattct tttgtcaata gaaaatcagt attatacaat atttatactg caccataatt
     6541 gttagagcaa aaatctaatg cttgagatgt ttatgtatga ctaaatatga ttttaatttc
     6601 aaattaaaag tagctaaatt ttatttgaat agagaaggaa gagatatttt aatagtaaaa
     6661 caatatggag tttatgacca ttctcaagtt gaaagatggg ttaatatatt aggagagagt
     6721 gaactaaaaa taaaaaaatt ttctatacta tatggttcaa gttagatgtt ataaactata
     6781 tgatgataac taaatatagt gtacaataaa tcgctaagta ttttggaata aacaatagca
     6841 cagttatagc aacatgaaaa taaaaatttt tacatagtgg tgttgatgcc ttttctaaaa
     6901 tgaaaggaag accataatga ataaaaacaa aaacactaaa gaaaaaataa attaacaaga
     6961 gaacaagaat tagaatatga aaataaattt ttaaaagatg agcttgagtt tttaaaaaag
     7021 atgtgtgtct cagggaagcc acattaaaaa acagtggtat cattattaat caaaagaaag
     7081 ttagatgtat tatcaaaaaa ttagaactta aatgcatagc attttttcat aaatcacata
     7141 aatataactc ctataaaggt ataattggaa aagtaaaaaa taataaaaca aatagaagat
     7201 ttaaaacatc aatagcatat cagaaaataa caaatgatat gctagaattt ataaaatata
     7261 tctaatgata aaaatggaaa atcaattata aaaaaggcat atctcgattc attttttgtt
     7321 atatagtcaa ataagaatac tatagatgaa agatacgatt ttgcatatgt attaagtaag
     7381 tattctgatg tttttggttg atacacatgc aataaagaag ttgtgtttgc agataaatta
     7441 gcaaaactat agtataagtt gcaacaataa agaaaatgaa aattatcata ctaaagcata
     7501 aggcatgtta caattttatt agataggaca ttaacataca ctaaagtact ttttattata
     7561 aagagtactt atttttttga aattcattaa tgtataaact atcaagaata ttactcaaca
     7621 taatttaaaa acaacttgaa taggtatttt taaattatgt gatataataa aaaatagaag
     7681 tgtatgccaa tacacttctg tagttataac taagcattct attcttgagg agtgcttttt
     7741 tcatttttat aaccaactct ttttacttta acaataatta tcagacctat atatttattt
     7801 ttaattgtct gatttattag ataaaaaagt tattatcata tttttatcat ttggtatttt
     7861 tattgaacta ccataatctt cttctaattt atctagacca cttttactta tttctccatc
     7921 tataattatg tttaattcta agtattcttc actaccatta gatgacacta aagtgtacga
     7981 accattataa ggcatgccta aattttctat tttatgtgcc aaagggtctt catcaccaat
     8041 accagagttt actgttattt ttccatcttt tttagtccac catattatgt ttccttcagt
     8101 atattttgat tcatctgcat agtagctaag agacgcactt tttattgcac ttatatcagc
     8161 ttcaagttta gctatctttg ctttttctat atttttaaat aatgctggaa gtgctactat
     8221 tgccaatatt cctattatag caattactac taataattcc actagagtaa atcctttttt
     8281 atttttcatt tatttcccct ccatataaaa ttaataatat ataaattttt attttgttaa
     8341 tttctacttt ctatatttta ccatatatag acaaaaaaat cactttctat gacaaatata
     8401 ctattatttt acaatttatg gaaatgattt aaaatgacat ttcttgttag aaaagatata
     8461 agaaatggat taaaataaag aaaactacag tcatatcaat gtcaataata ttatttaaaa
     8521 gcagatgaaa ataaaaagag gactcagaaa ataatgtcta tttaattata cttacttacc
     8581 aataccataa cttgatataa acaagattat actacatttt tttatgctct a
//
LOCUS       DAELTA010000155         8465 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.156, whole genome shotgun sequence.
ACCESSION   DAELTA010000155 DAELTA010000000
VERSION     DAELTA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.156"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(48..2945)
                     /locus_tag="KPG13_003006"
     CDS             complement(48..2945)
                     /locus_tag="KPG13_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0986704.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(3272..3880)
                     /locus_tag="KPG13_003007"
     CDS             complement(3272..3880)
                     /locus_tag="KPG13_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG0986705.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(4140..4685)
                     /locus_tag="KPG13_003008"
     CDS             complement(4140..4685)
                     /locus_tag="KPG13_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG0986706.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(4803..6626)
                     /locus_tag="KPG13_003009"
     CDS             complement(4803..6626)
                     /locus_tag="KPG13_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986707.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(6639..8414)
                     /locus_tag="KPG13_003010"
     CDS             complement(6639..8414)
                     /locus_tag="KPG13_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986708.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         2824 a         1464 c          765 g         3412 t
ORIGIN      
        1 taatattaaa gcacctttat tatatatgtc attatttaca cattgcttta ttctttttta
       61 aaagcgattt tttcaaactc ttttacaggc attggcttag aaaacaaata tccttgtacc
      121 atatcacacc caatttgctt tagaaattct acttgcgaaa tagtctctac accttctgat
      181 attactttca ttttcagttc ctttgccatt ttaatgacat tagatataac aattttttca
      241 ttattactct catctttttc tgtaaaaaat gctctatcta gttttaatat gtcaacaggc
      301 aattctttta aaagattaag agaggaatat cctgtaccaa aatcgtccat agagattaca
      361 aatccctttt ctttcaaatt gttcattgta tcaagtaatc cttctatatt atctaatact
      421 gctgtttctg ttagctctaa ttctataagt cttgtcggaa ttttgtattt atctacaatt
      481 aatttaaact cctctataaa attactattt acaaaatgca ttcgagacaa gtttacagat
      541 ataggtacta ctttttgtcc ttcatccatc cattctctaa tctttttaca cacttcctca
      601 aatacataga aatcaatatt tacaataaaa ccattcctct caaatacagg aataaattct
      661 attggtggta ttagaccttt ttgtggatta tcccaacgaa caagagcctc tgcccctata
      721 atttgataat ctgaaaggct atatttaggt tgaaaataaa caataaattc tccattatta
      781 agagcatcta ccattgaatt ttcaatttct ttctctttta atattttttt gtgcatttcc
      841 ttatcgtaaa aagcaaatga atttttatga ccacctttta tagtttttct tgcagtatta
      901 gctctatcca taatagtatt tatgtcatta tcacttttac ttattttata tataccacaa
      961 tctaaaatca atttataata gtttatttta ggattactaa gtatttgaac cttattgtaa
     1021 atattgttta accttgtttt aatatcatca tctgtcttat acttaataag aagaacaaaa
     1081 tggtcagcag aaactcttgc aaaaatttca tcttccttta attcacttgc aatagtattt
     1141 gatatatgta tcaaagtatc atttcccata tcatatccaa acatatcatt tatatattta
     1201 aatttgtcaa catctatata aaacaacgca tattcttctg gattattctt agcaaacagt
     1261 ttattagaat ttattttaaa tttatctata ctatctgctc ctgtcaatgg gtctgtatat
     1321 gctatcttcc ttagataatc ctctttcttc tttttattat aaattatata tttaatagca
     1381 attaagaata aaatacatac aaaagaaata atttgtattg aatattcttt aaaaatcaca
     1441 ccaaatggta ctttataagg tttgtcaata gtattagaat atatacaatc acttaattct
     1501 tcttcagtaa ttttgtctat tgatttattt aaaatagaca ctaatatagg gtcagtttta
     1561 tttgataccc ctatacgcat cccatagctt gtatctaaaa cagatataac agataaattc
     1621 tcactctttc cttctcttaa aagttcatca aactgataag aactaagtat cattaagttt
     1681 gctttacctt catttatagc gtttagacaa ctttctacac tcttataaga tgttgtctta
     1741 gcattttcaa aactcttttc tatataagaa tcaatagact tataactact agttaaagca
     1801 atatttaaat cattattgag attggtatcc aattttccta ccataatcat tggaatattt
     1861 atgtatggat ttgtcaattt catattaaat cgttttacct tatcattttc attgattgag
     1921 ccacatagca tagttgcctt accacttttt atgtaatcaa gactctcatt ataatttttt
     1981 gttttaatat attcaaattt tataccacat ttttttgata taattgacat taaatcggaa
     2041 cttatacctt taaatgaatt gctttcttca tcataatact ctacaggaga ccactctgaa
     2101 tcatatacca tagtaagttt tgggtttgct ttaataaaat tcatttcttg ccgagtaaat
     2161 gctatggaat tattgacctc tcttttgaaa tattttttat ataattccaa ctcataatac
     2221 atatcattta tttttaattc ttttaaagca taatctaatt cttttactaa attaggttta
     2281 tcttttgcag tagcaaaata aaaaggttct actgaaaatt tgctaactat tttcattcct
     2341 ttagcattta taatacttcc acaaacaaat gcatctgcct ttttttcatt gacggactca
     2401 attaattcat caatagaatt acagtatata tattcaactg aaaaattatt ttcttctcta
     2461 tacttctcaa atgcagtatt atgaaagttt cctctaagta cagctactct cattccctta
     2521 aaggaactta tatcttcata aaacatatta ttgttactat cctttgtata tagtgcacta
     2581 tattcatatc ctgaacttag tttaggaaaa ttaaatagtt tatttctttc atcattcttc
     2641 tgtaaaggtc cgagtaagtc tatttcaccc ttttctagtc tgtctaaaca ttcttcccaa
     2701 gaccctttta caaattcata ttcccatctt gtatattttg agatttctct tagataatca
     2761 taaccatatc ctgtataaga atgctctttt gatgaggata ttattccgta gtcatcacaa
     2821 tatccaactc ttattttttt tgaattttca gactcatcag caaagcatag tatattgtta
     2881 aaacatgata taataagtaa aaatatcaaa gaaagtatca agaagtgctt tttaaatgct
     2941 ttcatattta aaaacctcca atttgccaac ttactttaac ttatttcgtt ttctagttgt
     3001 aactactttt attgtaatgg taaatatatc aaattgcaat attctcatat aatattctta
     3061 ttcaactata atgaatttat tattatctat ttaaatacac tatctaatat tagtttctta
     3121 taagtcataa aagcacaact aacactataa ttctagtgat taattttatt tcaaaaaata
     3181 taataacact actaatattt taaatatcaa tagtgtcata aaaacttttc agattaatta
     3241 aatctttcta tatattttat aataatctaa atcatgctaa atatttagac agaaaacttt
     3301 tttctttaat agtaaatact tttgtttgaa tatcagaata attatttgaa tatctatcta
     3361 taaaaccatg atttgcatct aaaatctcaa tttggatatt ttgtttccta gaaagttggt
     3421 aagaaataga atcaacatca aatgaatcgt gctttgcaaa tactaataag gtaggacatt
     3481 ttggaattac atccatgtaa tctctaatcc gagagccata acaacaaatt atgccatcac
     3541 aaaaagaatc ttcactgcat ctccatgcaa tagttgctcc cacgctatac ccagcaacaa
     3601 atacatattt atactctgat tttaaatcat ttataagctt ttcaactttt ttataaatat
     3661 caaaaccaac attatttata aaaaaatcat atgcctcttg gacttttgaa tatggaaaaa
     3721 ctcttttttc tcccagaagt tcaggacaaa atacatcata tccatattta taatactttt
     3781 gacaaacatc ttctataaag ctattaatcc catagatttc atgcaaaaca acaatagcta
     3841 catcactttt atgtatatac tttatcagat tatccaccac ctttacaaaa cagcaaattt
     3901 tttatactta gtttaatata ttcttcttta catgtcaatt tacttttcta aaatcaaatt
     3961 tatttttgga ttaatcattt atttttaaaa gttgtgcatt attactatta aattattctc
     4021 tattattaaa aatataatcg ttagaaataa aaaataaagt acaaataatt aaaaaatatc
     4081 tcactcataa ctttaaaaat tataagtgag atactcctaa aatctacttt atattaaatt
     4141 tattttccaa aatatctatt taataatcct aaaaatgctt taccatgtct agcttcatct
     4201 ttacacattt catgtactgt atcatgtata gcatctaatc ctaattcttt tgctttctta
     4261 gctaatttta attttccatc agttgcacca tactcagcat caactctaac tcttaagttt
     4321 tcttttgtat ctgcaactac aacttctcca agaagttctg caaatttagc agcatgttca
     4381 gcttcttcaa gagctattct cttataagct tctcctactt ctggataacc ttctctatca
     4441 gcttgtctac tcattgctaa atacattcct acttctgtac actcaccaac aaagttagct
     4501 cttaatcctt cgattatttc ttcatctaca ccttgagcta ctcctattct gtgctcatca
     4561 gcccaatttt tttcgccttt catttcttca aatttatcag ctccaacttt acatactgga
     4621 cattgtgctg gtgcagcatc tccttcatgt atatatccac atactgtaca aacaaatttc
     4681 ttcataatta ctttcctcct aaattttata tatcaaatta aattttttat ttattaagtt
     4741 agtctatata tacccgcacc atataccttt aaacaatttt tttaaaagtt ttttctaaat
     4801 acttattttc cctgtgtcaa taatatgttt ctataataat gtccctttaa ttttatcaat
     4861 tctttatgat ttcctgattc cacaattcta ccattttcta aaactattat attatcacaa
     4921 tttgatatgg tacttaatct atgagctata attatacttg tctttccata agttaaatta
     4981 tttattgctt cttgaatttt agcttctgtt gctatatcta tgttacttgt agcctcatcc
     5041 aaaataagta ttggagcatt ttttaatact gctctagcaa tacttattaa ttgtttttga
     5101 ccttcactta aatcttcacc cctatccgat aatacagtac tatatccttc acgcatttta
     5161 actataaatt tatgtgcatt tgctatttta ctagcatata taacttcttc atcagtagcc
     5221 tccagatttc cataccttat attttccata actgtatcat tgaatatata accttcttgt
     5281 ggaactactc caaaacaagt tctcaaatag tttctgttaa tagagtatat atccttacca
     5341 tctaaaagta tacttccttc atctatatga taaaaatttg taattagatt tataatagtt
     5401 gtttttccac ttcccgtctc tcctatgatg gcatttgatg tgccagcttt gatttttagg
     5461 tttatattat ctaaaatttt cttttttgaa tcataagaaa aacttacatc tttaagctca
     5521 atttctccct ttacaaaatg agagtttaaa ttaccatctt tattatcgtt aatctcactt
     5581 ttaaacatgt tatgcacttc ttgctcttct tgttcatcta taatttcaaa aactctttca
     5641 catccagcta tggctgaaag taatgtatta aatagatttg ctaattcatt taaaggtctt
     5701 gtaaattgtc ttacataact taaaaaacta gctataaccc ctatggttat aatatcttta
     5761 gatgcaagaa tgcctcctat aatacatata gttgcaaatc ctatattact aatcacattc
     5821 ataataggca ttaaaagaga agtgtaaatt tgcgatttaa gggaaatatt tagaattcta
     5881 tcattgactt ctttaaaatc atttacagat tttctttcat aattaaaaga ttttactaca
     5941 tttatgtttg atatcatttc ttcaatatgt gcatttagtt ttcccaactc aacttgttgt
     6001 tgcttaaaaa aaactctagt tctctttgca ataaaattac tcacaaacat tactatcggt
     6061 attaatatta cactgcaaaa ggttaaaatt gggcttaata caaacatcat aataaaagtc
     6121 atcaatatgt ttaagattcc tgtaattaac tgtactaatg aatttgatat tcctgtactg
     6181 atattatcaa catcatttgt cactctactc ataatatctc cagtagaact tttatcaaaa
     6241 aaggttattg gtagattttt aaattttaaa aataaattac ttctcatttc tttaactata
     6301 ctttgagata atctagctgt acatatcccc ttagacagtt taactgaaaa ctcaattata
     6361 taaaatgaaa gtaaaataat tattcctttt aagaaaatgg attttgaata agtatcgata
     6421 tctattatgt caactacctt tccaatagta tatggtatta cagttgatat aattgaatca
     6481 attattacta gcaaaaaaat aaatctagta atcttcttat cttttttaaa gtacataaaa
     6541 attcttttaa acgttttttt aaaatcttta ggcttttctt ttttctttaa tctactgacc
     6601 ttctgatttc cagtacttaa attcaatata cttcctcctt aatttacatc ttcacttact
     6661 aaattctgca acgcaaaaat ttttttataa attggagaac taaccagtaa atcttcatgt
     6721 gtagcaaatc caaccacctc accctcatcc aagactaaaa tcttatcaca gtcaattata
     6781 gaactaatct tttgtgaaat agttattaca gtacatttct tactttcctt ttctcttaaa
     6841 ttacttatat actcttttat attctttctt ataattgtct ctgtattaat atccattgca
     6901 ctaaaacaat catcaagaat taatatttgt ggttttttgg ctatagctct agctatagta
     6961 attctttgtc tctgtcctcc tgataaattt ttaccccttt ggaataaaac ttcatcataa
     7021 gtattttcga gttttgttat aaattgattt gcatttgcta tttcacatga ttttacaaca
     7081 tcctctttgc atatatctct atttcccata actacattct ccaatatact ttttccaaac
     7141 acactactat tttgaaatac tacacctatt cttgccctta aactttcttc agaaatactg
     7201 tatatactac gtcctccaat cttaatatca cctttatcaa ttatatataa tttttgaatt
     7261 aaatttacta aagaagtctt tcctgaacca gtttgaccaa tcacaccaat attttgacct
     7321 tcttctattt taaaggatac gttttttaat attttttcat ctgtattttt gttatacgaa
     7381 aaatctacat tttcaaaaat tatatcacag tttacaaact ttaaatcttt ttctccaaca
     7441 tctaaacttt cttctctctc aattacttct tcattctcat caaccaaaac ctcacttatt
     7501 ctctcataag atgcttttgc cctaacaaaa acattaaaaa catgtgaaat cattataaga
     7561 gatgttaata ttctagtcat atagtttaca taagcaacta taatcccaat tttaatttct
     7621 cctgaataaa ttaaatgcct tccaattagt aatattatta ttatggaaac attaatgatt
     7681 attgtaattg tcggtctaaa aaatgacatc atagttgtag cttttctact atttttaaaa
     7741 agttcatcat ttaactcttc aaagctttgg ttctcataat cgtatctaca aaatagttta
     7801 actactttta ttccacttaa aaactgtctt aattttgaat ttactttatc tgtactttct
     7861 tgtacatttt taaataatct ataccctatc ttcatattaa gcaatattat aataaatata
     7921 attaaaactg caattagaat aattattgac atctttaaat ttaattttat agctataatt
     7981 aaacttccta tacacattat tggtgacttt ataaaaactc tcatcattcc attaacgaat
     8041 tgttgcattt gattgatatc attagtaatt cttgttatta atgaagctct accaaattta
     8101 tctatttgtt ttaatgagta tgtatttatc ttttcaaata catcctgtct taaatcataa
     8161 gaaaaatgct gagaaactct tgccgagatt acacttctta ttattgcaaa tattgcacct
     8221 aaaaaagtta tacctatcat tatagctcct gttgatagta tgtagtctaa gttcttattt
     8281 gaaaccccta tatctattat ttttgccatt atagttggtt gagctaaatc acaaaaagct
     8341 tccaatgtta aaaaaaatac tgctgttaga aataatacta tatatttttt tatgtatttt
     8401 ttaaaatacc tcattcaatc atcccttcat agaattttct cattaccaaa agtatatcat
     8461 aatgg
//
LOCUS       DAELTA010000156         8457 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.157, whole genome shotgun sequence.
ACCESSION   DAELTA010000156 DAELTA010000000
VERSION     DAELTA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.157"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            161..2020
                     /locus_tag="KPG13_003011"
     CDS             161..2020
                     /locus_tag="KPG13_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0986709.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2312..2911)
                     /locus_tag="KPG13_003012"
     CDS             complement(2312..2911)
                     /locus_tag="KPG13_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0986710.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3014..3811)
                     /locus_tag="KPG13_003013"
     CDS             complement(3014..3811)
                     /locus_tag="KPG13_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0986711.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3924..4029)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4121..5107)
                     /locus_tag="KPG13_003014"
     CDS             complement(4121..5107)
                     /locus_tag="KPG13_003014"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG0986712.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5591..7321
                     /locus_tag="KPG13_003015"
     CDS             5591..7321
                     /locus_tag="KPG13_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG0986713.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7431..7511
                     /locus_tag="KPG13_003016"
     tRNA            7431..7511
                     /locus_tag="KPG13_003016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7465..7467,aa:Leu,seq:tag)
     gene            7846..8361
                     /locus_tag="KPG13_003017"
     CDS             7846..8361
                     /locus_tag="KPG13_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG0986714.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3038 a         1054 c         1303 g         3062 t
ORIGIN      
        1 ttgtatatat aaaggactct aaagatgaac tatgtaaaac aaggtttata tattctaatt
       61 gtatagaaaa atttattgta aacgcatgta tattattaga aaaaatagtt tggaggtatt
      121 caataaacat aaaaaacaat ataggggata aaggtgtttt atgaagagat ttttgaggag
      181 aattatctta gttttattta ttttgttgat ttttataagt tttatttcta ttaaattaat
      241 tcataatgtt ggagattatg gaaaattgat taattatgtt gggattgttc gtggagctag
      301 ccaacgtcta actaaactag agatgaatca tcaaccaaat gatgaattaa tagaatatat
      361 tgatgaaatc ctacaggagt tgattacagg gcatggagat tatggtttag taatcactga
      421 ttgtaatgaa tacaatgagg acttactttt attagaaaaa aagtgggaag atttaaatat
      481 tgaaattaaa aaagtaagaa tgaaagaaca gaataaccag ttattaagta taagtgaaga
      541 gtttttttct cttgcaaatg atacagtgtt taaaatagaa aacttttcta aagaaaaaag
      601 taattactta atgactttaa taataattat atctattata ggtatattag catgtatcat
      661 attgatttta caatatagta aaaaaatggt tagactggaa aaattaaatg tagatttaaa
      721 aaatattgca tataaagatg aactaacagg agtaaatact atagaaaaat ttaaattgga
      781 tgcaaatcaa aatatttgta tacataagga taagaaattt gctgtattct atattgattt
      841 tgaaaacttt aaatacatta atgatatatt tggatatgat tatggtgaca tgatactgaa
      901 aagatatgct aatcttatga tgaatgacat agggaaatat gaaatatttg caagagaaat
      961 tgcagataga tttgttgcgt taagatgcta tgtagataag gaagatttag tggtaagaca
     1021 acgagatgta gacaatgaac taattaatat aacaaatgaa attaaaaata aacatagtat
     1081 tactgttgtt agtggaattt gttgtattga agatgtaaat gaaaaattaa gtatagatgg
     1141 tttaataaat agagcaaatt ttgcacaaaa aactgttaag aataaaccag gtactaatta
     1201 tgctttttat aatgacagta ttcgtaaaaa aatgattgaa gaaaatacta tcaaaagtag
     1261 aatacatgaa gctattgaaa aaagagaatt tatagtttat ttacagccta aagttaattt
     1321 acatagtcaa aaaataaatt gtgcagaagc tttagttcgt tggcttactc cggataaagg
     1381 tattatatct ccagcaatat ttattccagt acttgagaag aattttttta ttgccttagt
     1441 agataagtat gtttttgagg aggtttgtaa atggattaga aagaggttag atgaaaataa
     1501 accttttgtt cagatatcag ttaatgtttc aagaatacaa ttttataaca ctaaatttgt
     1561 agaaacttat tccaatatac aaaataaata caggatacct aaaaatacta tagaaattga
     1621 atttacagag agtgttgctt ttgagaatca aaaacatctt ttggaaatta tacatgattt
     1681 gcatgaaaat ggatttactt gttctttaga tgattttgga aaaggttatt cgtctttaag
     1741 tgttttaaaa gatttaccat ttgatgtttt aaagcttgat tctatgtttt tcaaggctag
     1801 tttagataaa gataaagaga aaatagtaat aaaaaatatt gtacatatgt taaaagaact
     1861 taatatcaca actgttgctg agggcattga atgcaaggaa caagttgaat ttcttaggga
     1921 tattgggtgt gatttagttc agggatttgt attttataaa ccaatgccaa tattagaatt
     1981 tgaaaaaata ttggataaag agtttgttta taatagctaa aatgtacttt ataaaatatg
     2041 aactaattaa gtaaaattat tttgtactga ataaattttt actttattta cagaataatg
     2101 attatttaca gaaaagcgcg aaaagtgtat tcctttaagt gtgggatgga tagcacaaat
     2161 agaggagaaa aaatattggc catataagaa tgcataataa tataaatatt taaaaggtca
     2221 gcattatata aaaattaaaa agaaaataaa taatttttct ataaaaaaga gactatgaga
     2281 aaatttactc atagtctcct aaataatgta tttattttct tcctgcaacc cattttaatc
     2341 cccaatgaaa ttcagtatcc atttctctat gacctttgaa gtatttaact actttttcaa
     2401 cactgaatgt ctcatcattt acattttcta tcaatgctat tgaacacata ttatagccat
     2461 ttttatgttc atcaagtttg acaactaagt ctgaatcttg tttttgtttg attgttacaa
     2521 ttccatcagc ttcagaccag ttagctacac cttcgtatat aaaagcataa ataagtattc
     2581 tttttatatc ttttatttta ttaccattta ttcttaaatt ttctccttgt gtattacttc
     2641 ctgttctgtc atctccatct aattgagcaa aaggtggatt gtctaaacta ccaaaggcat
     2701 ttcctaatgc ttgaacagca ccttttacac catttttcat ctcaaatagg caacccaaat
     2761 ctaaatctat attactgctt cttaaagagc caaaaaaacc ctttttttgt actttttgat
     2821 tccaatttaa attaactaaa atttcaccta aatcgctatt ttcctttttt gtaaggttta
     2881 ttttttgtcc cttttttaat tctatagcca tagtaatctt cctccacaaa catagattta
     2941 tttaaatggt agcacttaat aagtcaatat tcaacaagat tagaataata atattaaaat
     3001 tatactaaat atactaaaaa gtaggaatca caataccttt gtatttttct tcaattaagc
     3061 ttcttgcttt atctgatgta agaacatctt gtagaacttt aatcttttca ctgtctttat
     3121 cttcagttct aaatgctaaa agatttgcat actcgtcatt tataggagct gtataaattc
     3181 cgtttttctt ggcatcaaga ccagcaggta cagcataagt agtatctata aatgcagcag
     3241 tcacatctgg aagagaagat ggaatttgag caatatctaa ctctttgaat tgtattccct
     3301 ttggattttc tattatgtcc ttaggactaa ctaatccatc ccctttaggt aacgttaaaa
     3361 gtccaatact ttcaaggtat ctaaggttct tagaacaaga agatgggtta ttgcttattg
     3421 caattgtatc tcctttttta aattcatcaa cagattttat cttataagag taaagtattg
     3481 ctgggcatac atatagtttt ttaccagcca ttattttgta acctttggcc ttaacagctt
     3541 cttttagata aggttcatgt tgaaatagat ttgcatcaat ttcaccatta tttaaagctt
     3601 cgtttggaag tatgtaatca tcaaagtttt taacttctaa agtatatcct ttttctttaa
     3661 taagtggttt taattcttct aataactctc ctcctggaac taaagtagca ccaacaacaa
     3721 tttttttatc atctttagct ttagaacatc caaccacagc taaagttagt actagacata
     3781 ataacaaaca taataatttt ttgaatttca tataaattcc ctcctgaata taattgatta
     3841 gttaaaaata aaataccatt ctttatcaca ctgcaaaata aaaataagcc tgagtttaaa
     3901 ctcaggcttt aaaagctaat ttagactcat ctttcagtat aatactgcag gatttagcac
     3961 caatgtatat aaaaatatac tggttgctgg gtttcatagg gccagtccct tcaccactct
     4021 ggataagaag tatcaatttt taattatgtt ataaatatta tcatgatttt ttacgtctgt
     4081 aaacataatt ttataaatat tttaaaatct ttaatgtatt ttatttaaca cctaaaattc
     4141 cagaaactag aatatcagta tcagcaccta agaaatagtc atttatttca atagaatcca
     4201 gagtatttct caaagcattt tcttcatgcc ttataccaat aagtttattt tctacaaatg
     4261 caacatcaca ttttccaaaa aaatctccaa aaaattttat actagttatt atgcctttat
     4321 caacatttaa attaaactca acatttccac cagaatattt taattcattt gaaagagtat
     4381 attttggtga attaccaaaa ttccaatccc aagtttcgta ttttgattta gtaagctttt
     4441 ctatttgttt tatatcatgc tcactcaaac aataattagt attatcagta tttgttttag
     4501 aaagataatc cattatagca tctttaaatt ctaatatgtc catttttgaa tgtaaatgtt
     4561 cacttatatt ggttacacga gattttacag attttattcc ttttccttca aattttatag
     4621 gtttaacctt taatgctttt gagaggtcat ttatttcaga tgaaaataaa agagtcccat
     4681 gatgcattac tttgtttttg tagttgtatt gagcatttcc agaaaatttc ttgccatcta
     4741 taagaaggtc atttcttcca gaaaattcgg cattgacatc tagtgtttta agtaaatcta
     4801 ttatgggctg agtgaatctt ctgaaatcac taaaattatt attgttacaa gctataaatg
     4861 taaaatttat atttcccaaa tcatgaaata cagctccacc accagactgt ctcctaacga
     4921 caggtataga atattctttt atatattcta aatttatttc agaaagtgtg ttttggtttt
     4981 ttccaacaat tatagaaggt tcattcctcc ataaaataaa taagtcttca tcaaaatttt
     5041 ttagaagata ttcttccatt gctaaattaa aataagggtt tgttttttca ttataaatta
     5101 ataacattaa agtagttcct ccatagaatt gataaaattt ttattatgaa tacaattaat
     5161 tttataattc gacaataata taaaaactcc tttaaattag aaattttaaa aactaccttg
     5221 caaagtttaa ataaataata tatactcaaa atatgtttaa taaaagtgaa tagaagggta
     5281 gaggcgcaat aatctatcag tactaataag gagttaagca cagagagtta ttaggaaagg
     5341 agatattgcc gaagttttat aatcgatgct ttaagattat attgctgggg gtacatagaa
     5401 aatatgtaca actgtcacag gtttatttgt ggtgagctat catttaagga attatattat
     5461 atttattttt actattgttg atatattgta atgtttaagc cttgagtgtt taccactcaa
     5521 ggctttttta gttttattgt aacataatac cctgtacagt tcacaaaatt aaaaggagag
     5581 ggagatttat atgaggaaca aaaaaattaa tattattttt ctgacgacaa tcatgttcat
     5641 aatgtcaaca gtaatggttt ttgcagaaga agatatagac actattgcac tagccaatgc
     5701 agaaaaattt gggattttaa ctttaatacc accattagtt gctataatac tcgcatttat
     5761 aactaaaaac gtaataattt ctttacttat aggtattctt tctggtagtt ttataattaa
     5821 agctagtggt attaatgttt ttgcgacatt tatacaggca tttttagatt tagtagatag
     5881 agctttagta tctttagcag acccttggaa tgcggggata atactacagg ttttgtcaat
     5941 tggaggagta ataaatcttg ttgctaaaat gggaggagct aaagctatag ctgaagcact
     6001 tgctaaaaga gcaaagtcag caaaagggac acagcttata acatggtttt tggggctttt
     6061 agtattcttt gatgattatg ccaattcact aatagtaggt cctatgatga gacctgttgc
     6121 agataaaatg aaaatatcga gagaaaagct agcatttata atagatgcca cagcagcacc
     6181 agttgctggt ttagctataa tatctacatg gataggtctt gaggttggtt taatacatga
     6241 tgcttttgaa agtataagta tagatgtaga tgcatttggt atatttttaa atacaatacc
     6301 atttagattt tacaatatat taatattagc tttcattgta atatcagcat tattattaaa
     6361 agaatttggt ccaatgagaa aagctgagat aaaatctaga agtagaaaaa ttagtataga
     6421 ccttgatgaa ggcgtagaag agttggatga tttagctcca aaaaatggag taaaattgag
     6481 tgtatggaat gctataatcc caataggaac attgataata gttgcattag catcatttta
     6541 ttatagtgga tatacatcta taatgggagg agacgataag gctctgatac aactatttac
     6601 aaattctcca tattcttttg aagcaataaa agaagcattt agtgcatctg atgcatctag
     6661 agcattattc cagtctgcac ttgttgcaag tttagttgct attataatgg ctgtagtaaa
     6721 aaagattttt acaatatcag aagcaattga tgtttggata gatggaatga aaagtttagt
     6781 aatcactggt gtaatattaa tacttgcatg gtcattaagt tcagtaataa aagagttagg
     6841 tacagctaaa tttttaatac acctactatc aggttcacta ccaccattct tattaccaag
     6901 tttaatattt ggtttaggag caataatatc ttttgctact ggaacagctt atggaacaat
     6961 gggtatactt atgccacttg caattccatt agcatactca ttaaatcctg atatgtctta
     7021 tgtaatagta agtactagtg cagttttaac aggtgcaata tttggagacc attgttctcc
     7081 aatttcagat acaacaatac tttcttcaat gggagctggc tgtaatcata tagaccatgt
     7141 taacactcaa atgccatatg ctatatttac agcagtaatc actattgtat ttgggtatat
     7201 accagctgga cttggattgc caatatatat agtattacca gtggcaatag ctgctatatt
     7261 tgtaggtatt caaataattg gtaagaaagt tgatgaagcg gaaatagaac ttgtagaata
     7321 aagatataaa taagtttcac cttttattat cactattttg ttagttttta gataatttta
     7381 aaaagttatt gaaaataatg gcgaaatata gtaaaataaa agagtgataa gcggatgtgg
     7441 cggaattggc agacgcacta gacttaggat ctagcgccat tggcgtgggg gttcgactcc
     7501 cttcatccgc actttacagt acttaattgt actgaaaaaa actggggttg ctggctttcg
     7561 gagtgggcta tcccgttttt atttttttgt atatataata gattggcact ttagtataag
     7621 gtgctttttt tataggcaaa aacagtacat ttatttctgt tttattatca ttcatctatt
     7681 agaatatact ttagttaaga atgtgttaat aagcagtgtt cacattcttg ataaaatgtg
     7741 gaataatatg tatttttact aagacggaaa tgaaataatt aaaccattaa ataatattat
     7801 aagatgatga ataagtagat aaatcaaatt ttgtaggagg atattatgga aaaatatata
     7861 tggttgtttc cattactttt tatttttcat gatatggaag agataatagg ttttggaata
     7921 tggcttaaaa aaaataaatc aatgcttgat aagaagtatc catttatcag taaagtttat
     7981 gaaaattata gtactgaagg catggcattt gctgtgtttg aagaatttat tttatgcatt
     8041 attttctgta tacttacagt tataacagag aatcagtatg tatatctact atggttaggt
     8101 agttttatag catatacctt acatttggta attcatatag ggcagtcaat cataataaga
     8161 aaatatatac cttcacttat taccagtatc atatgcttac caataagcat ttggtgtata
     8221 agtaagagta tttatatagt tgattgtgaa atgagtacaa caattctgta cagtataatt
     8281 ggcattataa ttgtagcatt gaatttaaag tttgcacagt cgctgattgg aaaatttaca
     8341 aagtggatgt caaacatata aattttagtt tattggacag ttcaaggaat cagaaattgg
     8401 aattttatag tattaatatt ttatggtatt aatataatgt ataaaatttt atagcaa
//
LOCUS       DAELTA010000157         8285 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.158, whole genome shotgun sequence.
ACCESSION   DAELTA010000157 DAELTA010000000
VERSION     DAELTA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.158"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(96..272)
                     /locus_tag="KPG13_003018"
     CDS             complement(96..272)
                     /locus_tag="KPG13_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986715.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(376..984)
                     /locus_tag="KPG13_003019"
     CDS             complement(376..984)
                     /locus_tag="KPG13_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0986716.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(1083..1907)
                     /locus_tag="KPG13_003020"
     CDS             complement(1083..1907)
                     /locus_tag="KPG13_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG0986717.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(2085..2978)
                     /locus_tag="KPG13_003021"
     CDS             complement(2085..2978)
                     /locus_tag="KPG13_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0986718.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(3235..3672)
                     /locus_tag="KPG13_003022"
     CDS             complement(3235..3672)
                     /locus_tag="KPG13_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG0986719.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(3929..4078)
                     /locus_tag="KPG13_003023"
     CDS             complement(3929..4078)
                     /locus_tag="KPG13_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG0986720.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(4091..4321)
                     /locus_tag="KPG13_003024"
     CDS             complement(4091..4321)
                     /locus_tag="KPG13_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG0986721.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(5180..5401)
                     /locus_tag="KPG13_003025"
     CDS             complement(5180..5401)
                     /locus_tag="KPG13_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG0986722.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            5981..6238
                     /locus_tag="KPG13_003026"
     CDS             5981..6238
                     /locus_tag="KPG13_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0986723.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            6707..7945
                     /locus_tag="KPG13_003027"
     CDS             6707..7945
                     /locus_tag="KPG13_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG0986724.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         3101 a          947 c         1094 g         3143 t
ORIGIN      
        1 aataagaccc tgtaatataa taaaaatact ataatatagt aaaaatcatg caataataaa
       61 aatctataat aataaacctc agcataacaa agacattaat ttcttactaa aaaggccacc
      121 acaattaaca gtgaaaaagc aattgaaagt attgaaaaag taaagtgaga attagagtca
      181 ttattacgat tattaagttt tatccataaa ccacatataa ttgtacacaa caataaaatt
      241 gctgatatta ctcctataaa tgtaaaaaac attaaaattc ctccccctta tttaatgcaa
      301 gtatttttag tcaatgagaa tattttcaaa cgttaccata aaataaaaaa atgtacataa
      361 aaaattaata tagtattatt ttgtaatatc taccaataat aaatctaatt gaaaatctat
      421 taaaccattt ctctgttcaa tattaagaat atcaatacca ctatacttga aaaaagcatt
      481 agaacgaaga aaaagcaact gaataaatga agttatttga tacgcaatag ttctgtattc
      541 agcttcagtt tttttgttat tctgattttc atagtgagcc ttaattaaag gtagtatctc
      601 aaatggataa acaatttctt cctgtatcaa tatatagggt acagaaatct ttataaattg
      661 actatgttca cctataaaat caaaaaatgc aattagtatt tcccttaatt gctgtttagg
      721 tgctaaattt ttagtatcat gtgtagcctt atattcatcc atatgacgct ttaaaaattt
      781 tttaatagaa atattaatta actcctcttt agatttaaaa taataattaa tcatagctaa
      841 attgacattt gctttagcta caatttctct agccgtaatg cttgaaacat ctctatgtga
      901 agatagtaac tggattgtgg cattaattaa ttgttccttt gttgaatctg ctttcataat
      961 ttttcccctt aaaagtttct ccataaaaaa attaaacatg tttaatacac gtttagatac
     1021 tatcatgaaa attttgggat gtcaatgggt gattagtact taagtattta agtactaatc
     1081 tattagactt ttataagggg gatttgaatt tctgtaagat aattttcagg atttatttca
     1141 ttatgttcac ctttatgtcc gatttgtcta tttatacctg atatttgata atcactattt
     1201 ttatctaacc aataacagaa cgattcatat gcaccagcta tattttcata tggaccataa
     1261 accatagtac atgccatcat atcaatttct tctgtctcac gatatataaa tccagatttg
     1321 ttctttccaa tcttctttac aaccattcca acttcaatat ctacaccatt atctttgact
     1381 tcttcatcat ggtaaaaagc tatgttattg ttggattgac gtgatacttc tatatgttct
     1441 tctttgataa atttagatag cttttcccag agtatacctt cagattggta gtctggaatt
     1501 atctctctaa gtgatatgat tttataactt ggaatctttt taaagacaac attgtaatga
     1561 atttcatctt tatcacagtt tatagcttct ataaatttat caattttatt tattctttgt
     1621 tgttcctgct ttatttcctt ttgtatttca ttcttttttc tattcaattc cttaattaca
     1681 aattcatcat tccaattatg tacgatattt gctatttctg caacgctaaa ttttgaatct
     1741 ctcaataata caattctttg taatattgaa atctgttcaa ctgagtataa tctatgacct
     1801 gtatatttgt ctgtttttgc aggttttaaa ataccaagtt catcataata acgcaacata
     1861 cgaatagaaa tttgtgttag ttttgaaacc tctcctattt taaacatgaa aatatgacct
     1921 ccttttcact tataatcagt atatattttg tttttatata cttcaagcgt ttgtggaaat
     1981 acaattattt tccagatgtt gcaataaatt atagagagta aattatattt aagtagtaat
     2041 cgtatcaaat catagattat cttaatttat aattcatatt aaaattacca gtctaactca
     2101 aattcaaaat catccgactt tatctgttca atttcagaat ttaccttctg tttaaattta
     2161 ttagaagaaa tcatatatac aagaaatatt aaaccaaata ttatttgtac atattgagct
     2221 attacacata atttaatagg gtagtgtaga agtaattgaa tgttgaaaaa atagtacatc
     2281 atatgaacta atccagctga taaaatgctt ctacttgtat tgatatatac aaaattaatt
     2341 ataaagttta aaagtacaat gtttggtata aataaaaaag catcaaatag ggagtattgt
     2401 gccaaattat atggtatttg attaggtgta aaatacgacc ctagatacca gattccacaa
     2461 acaagcccta aaataattga agagcctata aatccaaatc taactaataa tttgtcaatg
     2521 gcatatcctc tccatccagc ttcttgacta agtatactca atataaaact tgctaatagc
     2581 atataagcta ttttagaagg tctaaacact gctatgtgta tccaatctat atcagaagtt
     2641 tttacattaa agtaatttcc gattattaac gaagtaataa aagttaaagc aaaaaataat
     2701 attgttaata gtatccattt tattccgatt tctttaaagc ttaggtatct ataaataaaa
     2761 tcttgttttg cactttgtgg atagattgta gaaataaaaa atagacctac aatagctggt
     2821 gatgtatatg caagataaaa aatgatttct ccaagtattg tatttgtaat tccgagcaac
     2881 gcaggtgtaa gactgaatat ccagttcaat actagagtaa atgtaaagaa atagattaag
     2941 tgtttgtttg tattaaaggg ggtgttatat agttgcattt tttcactatc cttccgtttt
     3001 ataatgaagt tttaattttt attaattgtt tatttatttt tatatgtatt aagttatcgc
     3061 ttgatgttag ttattgccta taataatatc ctatttttta tattttgatt aattattttt
     3121 acactaaata taattaatta gtatttaata ttgaatatat tatattatta ttaattatta
     3181 aataggtcaa taaatttgca attagtgata ttaataaatt actttagatt tttattaaat
     3241 gtttagcttt tctttaatat tttttatata acgacgattt ataataactt tttcgttgtt
     3301 ttttaaagta gcttgcatgc gattatgtga tattactttt atacttttta tgtagtccat
     3361 atttaaaatc atggatttag atattcgtga gaaagatgta ttagctaata attttgataa
     3421 tttatatatt tttaaatcaa tttcaaatac atcatcttca taataagcaa atgttttatt
     3481 tccaactgtc tcaaagtata aaatatcaca gacattgagt ttataaatct ctcctgattt
     3541 tttacctttt atatcaagta acggtatact caataatgaa attatttttt caatgttatt
     3601 atctagagca gaacatttta tggttatttc aatatcatca caattaatac cttgttcgac
     3661 atgtattttc attaatcatc acctcatttt attactgtca ttagaatagc attatatctt
     3721 attgagtcca actagatgcg ttctaaattt caaattaagc atagtgaaat tcaaaaataa
     3781 aacagtgaaa ttcaaatttg aggtggtaaa attcaaaaaa atactatgga tatttggata
     3841 aattatccaa atatcctagt atttcacata gaatatgtct aattatttta caaatagacc
     3901 tattttatga cttacatata ttgtcaaatt acttcttaaa tatcaaaata tttttggata
     3961 atgtatcaat actgtttgat aaagtattta atttaccctc aattctaacc aacaagtaca
     4021 tggatagagc tataggaaaa ccaacattgg ttattaacag ttgtaaacta tcatccataa
     4081 aattagcccc ctattctata actaagtcat aaggagtggt atttgtttcc acaaccttgg
     4141 cttcaacaac agaagctagg tcaaatcctc caaccgaaaa aatattttta gatactatca
     4201 aatccatgac agattttata ttttcttcag ttaaatcatc ctttggattt tctatagaaa
     4261 aagaaacttg tttatccttt acatttttaa agctcatgac taattttttc ttaatttcca
     4321 tataattacc tccgatataa taatttatat tttaattgtt tgcttgttaa tattggtatt
     4381 ttaattatca atattaatgt tttgcttgtt agtattaata tttcaattgt caatattaat
     4441 gttttgcttg ttagtattaa tatttcaatt gtcaatatta gtgtcttgct tgttagtatc
     4501 agtgtttcaa ttgtcaatat tagtgttttg cttgttagta ttaatatttc aattgtcaat
     4561 attagtgttt tgcttgttag tattaatatt tcaattgtca atattagtgt cttgcttgtt
     4621 agtattaata tttcaattgt caatattagt gttttgcttg ttagtattag tatttcaatt
     4681 gtcaatatta gtgttttgtt tgttagtatc agtgtttcaa ttgtcaatat tagtgttttg
     4741 cttgttagta ttagtatttc aattgtcaat attagtgttt tgcttgttag tattagtatt
     4801 tcaattgtca atattaatgt tttgcttgtt agtattagta tttcaattgt caatattagt
     4861 gttttgcttg ttagtatcag tgtttcaatt gtcaatatta gtattttaat tgttgactct
     4921 agttattgct gatatagaaa atatttaatt tatttcaatt aattagatta aagtagggta
     4981 gcatctaaaa tagctaacat gtttaagttg aaatacgttt aatatattaa tttttcaaag
     5041 cgttaatata ttaaagttat aaccaaaatt gaagttataa tctgccaaat ctatagcgta
     5101 ttaagtgcat tatataactt aatcttttat ataaagagtt aattttttaa tttgtaaagc
     5161 agagtacgtt taaaagacac tatttagata gtgatgtact atctaatttt aaaatatcta
     5221 caatagggtt ctcttgaaga cttgctattg cttctgcaac agcataaaca tcttcattta
     5281 atgcctctgc ttttacattt gcaaatgatt tacttttaac aactgcctta cccttatcat
     5341 caaaaccagc atccaatttt agttttatac tagataaatt ttttgttgaa acaacttcca
     5401 taaaattacc tcctaaaaat ttaatttcaa agatttgtcg actttctttg ttagctataa
     5461 tactatcatc taagataata tcggagttaa acagtaattt ctaaagattt tttgtgtaaa
     5521 tttttatagg aaaaatttta cttttttgct gatgaaaaag tttacaagtt atttgtgaag
     5581 atatgttctt agtgttttga aatagtattt gtatatattc atgcttagtt gagatagttt
     5641 tgtatggatt ttcatatgtg tagagttaaa tgttaggtac taaagtttat gttaaattaa
     5701 atgttaaagc ttatgttaaa aattttatta aatttaatct gtaaataaaa aagttaaaag
     5761 tccaatatta acattttact tttaaaccat taaaatcttt attaaagcta acaaagttat
     5821 aagttatgag aaattttatt gaagttaata caaaaattaa ttttaaagct aagaaatttt
     5881 ttattagatt caataaaact aataaatata gtttagtcaa taatgtatta aataaaaact
     5941 ttgttaagtc tataaaaata tcttaaagga ggtgtaactt atgaagataa aagtggagga
     6001 tataccagtt caatttcatg caatggtaga aataatcgga attgataagt tcatagaggt
     6061 tgccaagttg tatggaggaa caaatactta tatacctacg tataaggggc tttttagata
     6121 tgctagaaat agggatatag taaaacaatt taatggagtc aaccacaatg aattggctat
     6181 tagatataat atgtgtgtaa gcaatattaa gcgaatactc aatgaaaata gtgtttaaat
     6241 ataataaaat aatgtttaaa cataatgaaa atagccttgt taaacgtaag agtaaagaca
     6301 aagaagatgt tttataaagt ttttttagac aaattaacta aatttactac ttattaattt
     6361 aaatacattg tatattctat aaaaaaattt gttaaatact ggagatatat tgagaataca
     6421 aagaaaatgg tcgaaaatat ttgttggcta aaaaatagat aaaatgatac aattgcttgt
     6481 ataaagggga aaaataaata aataaactat ataaataaag aaatagcaat taatatgaca
     6541 taatatattt ataaaattaa gaatataaat agatatttta agggattagt tgttaaatta
     6601 ggaaatatta aaaaaaatat atatttatat ttaaactatt ttgttattta tataaaaata
     6661 cttatctagt tactagcaat tattagtaga aatgggggaa atagagatga ataagggatt
     6721 ttacaaatta aattcagtgg tattgaactt tgatattaag aatgatgtta acaagataag
     6781 atttaatcat tatataatga gaaaagaaaa catacaaaga cttcaaggaa ctctgccaaa
     6841 cggacaattt tatatgacag tgagaagagc tgctacagat ttacaacttt ctatttctac
     6901 tataagtagg cttgtaaaag agtttttgga tttagggata ataagattag tgtctaaggg
     6961 tgtaaaaggt aatcgttctg tatatagtta cgtaagtata gagtttaatg gaagtttatt
     7021 tgatgatagt aaatatatgg aacttgactg tgtatgtaat gatgaattta ttgataatag
     7081 tggcatagag tttgattgtg atagcaaaga taattctttt aatggagata taaatgtaga
     7141 aactgtgcat tgcattaatg agtatttctc tgatgatgat gtagagcata attataacaa
     7201 tttccataac aaagatacta taaaggataa agccatttct aatattagca ctatcaaaaa
     7261 atatattgat gaatcttttg atacaaataa atcagaaaat tctaacttac aacaggagtc
     7321 aataaatttc aactttaatg atacatgcaa tgaaatagat aaaagaacga ataatagtat
     7381 agaaaataat caaaataaaa ggcctatgag attgagtgac atcagtcttt taagtgataa
     7441 aggtatgaca aataaaaaga attactttaa tcataatcat ggtatacaaa aagggactaa
     7501 atctgtaaca aagaaaaaag aatccttaaa taaatttaat aaaaaaaata tacagcaaag
     7561 cattataaat atgctaaata aaaaatcagg taagaatttt aaaatcgatt ctccaataag
     7621 cataaaactt atcaatgaaa ggctaaagga aggctatgtc ttagaagatt tctacaaagt
     7681 tattgaggta aaagtggcta agtggaaaaa taccataatg gaaatgtata taagaccaga
     7741 aactttgttt agctataagt ttgaatccta tgtaaatgaa aacatatcta tgaaaagtca
     7801 aagtaatgtt cacccatgta aagaaggcaa tagtaatgat agcagtaatt atatacattc
     7861 tgattatata acatatgatg gcagtgtgcg tgagagaaaa aaatatgaga atgtaagata
     7921 ctggaatcct atatgtgaaa tttaattagt ttaaaaatgt atttatgata aataagatat
     7981 taatagagaa tagtgagcta tagcaaatga tatatcaata gttggtagta agttatatta
     8041 agtaagatat tactatcagg tagtgaatta tattgaacga tatattaata attggcagta
     8101 agttatatca aataagatat tactatagag taatgaatta tattgaacga tatattaata
     8161 attggcagta aattatatta aataagatat tactatagag taatgaatta tattggacga
     8221 tatatcaata gttggtagta agttatatta agcaagatat tactatcagg tagtgaatta
     8281 tattg
//
LOCUS       DAELTA010000158         8168 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.159, whole genome shotgun sequence.
ACCESSION   DAELTA010000158 DAELTA010000000
VERSION     DAELTA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.159"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     regulatory      210..314
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            524..2998
                     /gene="gcvPA"
                     /locus_tag="KPG13_003028"
     CDS             524..2998
                     /gene="gcvPA"
                     /locus_tag="KPG13_003028"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG0986725.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            2998..4455
                     /gene="gcvPB"
                     /locus_tag="KPG13_003029"
     CDS             2998..4455
                     /gene="gcvPB"
                     /locus_tag="KPG13_003029"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG0986726.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            4878..7235
                     /locus_tag="KPG13_003030"
     CDS             4878..7235
                     /locus_tag="KPG13_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG0986727.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(7321..7638)
                     /locus_tag="KPG13_003031"
     CDS             complement(7321..7638)
                     /locus_tag="KPG13_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG0986728.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         3245 a          873 c         1420 g         2630 t
ORIGIN      
        1 tctttgtgtg ttattttaat aataaattaa gtgaaaatgt tattttacat taatatataa
       61 tatctttgca taagagtgaa taatataaaa gagaaataat tgataataaa aacttaacaa
      121 aaaaattaat acataaaaaa aaataaatat aatgtgttga catttaattg aaatattcat
      181 acaataaaat tgaaaataaa tatttccaaa tgagattagc gagagagtcc tttatacgaa
      241 aaggcgccga agaagaaatt taagaaatgc caatttttag agaagctttc aggcaaaaat
      301 atcgctaatt gatgggactc tggagagact taagttaaga tactataagt caccgaagaa
      361 gcaaaataat attaaattat taaatctttc aggtatacaa acagggatat ataggctagg
      421 attattttcc atgctgatat atcttttttt tatgaaaaag tacaggaaat aatatcaaag
      481 ttacttaaat acagtaggtc taattttcta gggagggtta tatatgaatg aattgaaaag
      541 agtatctttg tacaatatac acaaagagct tggagcaaaa cttgttgaat ttgctggttg
      601 ggaaatgcct ttagaatatg agggtataaa taaagagcat gagaaggtca gaaaaagtgc
      661 aggtatattt gatgtatctc atatgggaga ggtacagata aagggagcag agtcagaaaa
      721 atttatacaa aacctagtta caaatgatat aagcgcatta aagataaatg atattatata
      781 tacacctatg tgttatgaaa atggtggagt agttgatgat ttattaatat ataaatttgg
      841 tgaagaagat tatttattag taataaatgc aggtaatata gataaagatg tagcatggat
      901 aataaaacaa agtgaaggat acaatgtaga tataaaaaat atatctagtg aagtgagtca
      961 attagcaatt caaggaccta aagcagaaga aattcttcaa aaaatcacag atatagactt
     1021 aaactcaata aaattttata agtcaattcc atcaacaaaa gtatgtggtt gtccttgttt
     1081 agtttcaaga acaggatata ctggggaaga tgggtttgag atatactgta aaaataaata
     1141 tgtagaaata atttggaatg aagttctaaa ggttggagga gaagatatat gtccagctgg
     1201 tctaggttgt agggatactt tgagatttga agcagctcta cctctatatg gacatgaaat
     1261 aaatgaacac atatctccaa ttgaaggtgg tttatctatc tttgtgaaaa caaataaaga
     1321 atcatttatt ggcaaatcaa ttttatcaaa agaaaaagaa agtggagcta aaagaaagtt
     1381 agtaggattt gaaatgcaag gtaaaggaat gcctagaaat ggatatgata taaggattgg
     1441 tgataaaaca gttggatttg taacaacagg atgtgcatct ccaacgacag gaaaaatttt
     1501 aggtatggga attatagatt ctgaatatgc aaaagtagga aatgaaatag gtatagcaat
     1561 cagaaaaaaa gttgttcctg ctgtgatagt taagaaacct ttttataaaa aacaatataa
     1621 aaaagataat attatattaa acaaagaaaa taaattttca tatatacctg ctacaagtga
     1681 agataaatca aaaatgttaa aagtagttgg attaaattca gttgatgaat tattttcaga
     1741 tataccagaa gaagtaaaat taaaaagaga tttaaatttg gaaattggaa aatctgaatt
     1801 agaggtaagc aaaatagtaa aaagattgtc agaagaaaat ttaagtttag aagacttgac
     1861 ttgttttctt ggtgctggtg catatgacca ttatatacca tcaattataa aacatataac
     1921 ttcaagatct gaattttata cagcatatac cccatatcaa gctgaaataa gtcagggaac
     1981 tttacaagta gtttttgaat ttcaatctat gattgcagaa attacaggta tggaaattgc
     2041 aaatgcatca atgtatgatg gagcaactgc tgcaatagaa gcatgtataa tggcaatgaa
     2101 tcagactaga aaaagtaaaa tagtagtgtc aaaaactatt catcctgaaa ctctgtcagt
     2161 attaagaact tacttacaat ataaagattg tgagatagta gaaattgact tttgtaatga
     2221 atatgggact acagatatag aaaaattaaa agcttctgta gacaaagata cagcatgtgt
     2281 tcttatacaa actcctaatt tctttggaat tattgaagaa atggaagaaa tagaaaagat
     2341 aactcatgaa aataaggcta tgttaattat gagtgtagac ccaatatcat taggagtttt
     2401 aaaaacacca ggtgagatag gtgcagatat tgttgttgga gaagctcaat cacttggaaa
     2461 ccctcttaat tttggaggtc cttatgtagg atttttagct agcaaatcta aatatactag
     2521 aaaaatgcca ggaagaatag ttgggcaaag tttagatgta gaaggtaaaa ttgcatatgt
     2581 attaacttta caaactagag aacagcatgt aagaagagaa aaagcaactt ctaatatatg
     2641 ttcaaatcaa gctttaaatg ctcttgtagc ttcaatatat atggctacta tgggtaaaga
     2701 aggatttaaa gaggttggta tgcagtctat gaaaaaagca cattatacct acaataaact
     2761 tgtacaaact ggaaaatata agccaatatt taaaggtaag tttttcaaag aatttgctgt
     2821 acaaggaaat cttaatatag aaactataaa tgataaactc ttagaagaaa atatattagg
     2881 aggttataat ttagaataca attatccaga attaaaaaat tcaactttgc tttgtgtgac
     2941 tgagaaaaga agcaaagagg aaatagataa gttagttgga ataatggagg gattataatg
     3001 aaagaataca atagtttgtt gatagatata tctaaaaaag gtaggaaagc atactcactt
     3061 ccaaagttag atatagatga tataaaaata gaaaatatga ttgaccaaga tatggcaaga
     3121 caaagtgagt tgaatttgcc agaagttggt gaattagaat tagttagaca ttatacatta
     3181 ttatcaaata aaaactttgg agttgataca ggtttttatc ctttgggttc ttgtactatg
     3241 aaatataatc ctaaaataaa tgaagatatg gcagcacttc caaactttac tggaatgcat
     3301 ccataccaat cttcggatac agctcaaggt tctctttctt tgatgtatga tttatcaaga
     3361 agacttgcag aaattacagg tatggatgaa gttacactac aaccttcagc aggttctcat
     3421 ggtgagttta cagggcttat gattataaaa gcatatcatg aaaatagagg tgaccataag
     3481 agaaccaaag tgataattcc agattctgca catggaacaa atccagcaag tgcagctatg
     3541 gcaaactttg atgtgattca aatagcatct gataaaaatg gagctgtaga cataaatgca
     3601 ttaaaagagg ttttgaatga tgaagtagca gctcttatgc ttactaatcc aagtactctt
     3661 ggtttatttg aaaaaaatat aaaagaaata gctactcttg tacatgaagc aggaggactt
     3721 ttgtactatg atggtgcaaa catgaacgct attatgggga taacaagacc tggagatatg
     3781 gggtttgatg tggttcatct taatcttcat aagacttttt caactcctca tggaggtgga
     3841 ggtccaggag ctggtcctgt tggagttaaa aaagaattag tacctttttt accaatacca
     3901 gtaattgaaa gaaaagaaga taagtatgta ctaaattatg atagagaaaa atctattggg
     3961 aaaattaaaa atttctatgg taattttgga gttcttgtaa gagcttacac atatatacta
     4021 actatgggaa gggatggact taaagaggct agtgaaatgg cagttttaaa tgctaattat
     4081 ataaaggaaa gtataaaaga tgattatatt ttgccaatag ataccttgtg taagcatgag
     4141 tttgtattag gtggattgcc aagaggagag gccaatataa aaactatcga tattgcaaaa
     4201 agactactag attatggata ccatcctcca acaatgtact ttcctcttat tataaatgag
     4261 gcacttatga tagaacctac agaaagtgag agtatagaga cattagatag ttttatagaa
     4321 gctatgaaaa gtgttgctaa agaggcaaag gaagaaccag aactattgaa aactgctcca
     4381 cataatactt tggtcaaaag agtagatgat gcaagggcag taaagaaacc tatactaaca
     4441 tggtcacaaa gataaatagt taaaaagtag agatttaaag tataaagaga attaaatttg
     4501 taagaaaaag ctatctaact taatctagtt tagatagctt ttttgtatat tagtatgtat
     4561 ggtattttct atataaagtt ttaaattata ttagtgctta tatagaatat tgatatgatg
     4621 attatgtata gatattaata agaattgttg aatttatctt gccttaactt tatttactct
     4681 tttatattaa gaaaaactta actaaaatat agcgaaattc ataaaaattt aggaaatctt
     4741 aagaattata atatttaaat ctttagattt atattttatt atataaacta tagaataata
     4801 aaataaaaaa attgagaatt ttataatata tttgtagatt tgagtataaa aaagtgctaa
     4861 gttgaaagga gtaaaatatg gataaggtta atttagaaag gtatagttgt gatataaatg
     4921 ttggtttaaa taaaaatcaa cttcaaagta gaatagatga taatttaatt aatgcatttg
     4981 ataatggaaa aaccaaaaca tataaacaaa tttttaaaga caatatattt acacttttta
     5041 atctcataaa tattgtttta gtctgtttat tagtttcagt tggttctttt aaaaatacct
     5101 tatttatatt tattgctgta attaatacta ttataggagt aatacaagag ataagagcta
     5161 aaagattatt ggacaatcta tctgttatag tttcaaataa ggtatctgtc ataagagaaa
     5221 aagagaaaaa agagattgat gtacaagaat tagtattaga tgatattatg attttaaaaa
     5281 caggtaatca aatttgtgca gattcaaaag ttttaaatgg aaaattagag gtaaatgagt
     5341 ctttggttac aggtgaatct gatattatta taaaaaataa aggcgatttt ttatattctg
     5401 gaagttttgt agtttctgga gaagcttttg taagagttga aaatatagga aaagataact
     5461 atgcaaataa gatagctaat gatgcaaaga tttctaaaaa acataattct caacttagaa
     5521 attcattaaa tgcaatatta aaaattgtag gtataataat aattccttta ggaattatat
     5581 tgtttgcaaa acaacatttt ggaaatggag attctatagc tacatctgta gtaggtacag
     5641 tggcagctgt aaatggtatg ataccagagg gattaacatt acttacgagc attgctctag
     5701 ctgtaggaac tataaaactt gctagtcata aaactttagt gcaagaactg tactgtgtag
     5761 agactcttgc tagagttgat acactatgtc ttgataagac aggtacaatt acagaaggta
     5821 aaatgcaagt tgatgatata gtaatgttgg aagaagtgga tattaatgaa ataatgggga
     5881 atatatgtaa ttctctcaaa gatgataatg caacactaaa tgcaataaga gataagtata
     5941 atgttttaga tacgtataaa gctaagaatt taataccatt ttcttcagaa agaaaataca
     6001 gtggggttac ctttgaaaat aaaggtacat atttattagg tgcatttgaa tttatattta
     6061 aggaaaaaaa taaaaaactt aggatagaag ttgaaagata ttctaaaaaa ggaaacagag
     6121 taatagtttt agctcatagt gattcttata tggaagataa taatattcct gaagatataa
     6181 agccactagc ttttatattg atattggata aaattagaga tgaagctaag gaaactttag
     6241 aattttttta caatgaaggt gtagatataa aaattatatc tggtgacaat cctataacag
     6301 taagtgaggt agctagaaaa gcaggtctta aaacagctag taattttatt gatgcaacaa
     6361 cacttaaaaa tgatagtgat atatacaaag cagttgataa atacagtgtt tttggaaggg
     6421 taagcccaca acagaaaaaa caaatgattt tagcattaaa aaaacaaaat catacagtag
     6481 ctatgactgg agatggtgta aatgatgtgc tagcacttaa agaagcggat tgtagcattg
     6541 ccatggcatc tggaagtgat gttactaaga atgtgtctaa tttagtctta ttagattcaa
     6601 actttgcatc aatgccaaag gttgttatgg agggaagaaa agtaattaat aacattcaaa
     6661 gagcatcttc tttgttttta gtaaagacaa ttttttcatt cttcttgtcg ttattaacat
     6721 tattcttctt cagtagacag tatccatttg ttcctataca attatcactc attggggctg
     6781 taacagtggg tataccatca ttcttcttag ctcttgaagc aaacaaaagt agaatttcag
     6841 gaaatttttt actcaatata ttaaaaaatg ctttaccagg agcactatgt gtgacagtaa
     6901 atgtaattat tgtttacaat atagttgaat atttaggtta tagttcaagt gtattttcca
     6961 caatgtgtgc tatattaact ggtgtatgtg gtcttttaat attaagacaa caatgtttgc
     7021 catttaataa agaaagatta tttttaatag taagtatgac tattgcattt gttattggga
     7081 ttctattttt aggtggatta ttctcatttg tagcattgag tagagaatta attctcataa
     7141 ctatggcatt agcaatatta atgccaatta tgataaaagt tatgttattt gtcttagatg
     7201 agatattaga gacaattgtt ccagatttaa attaatttta aaatgatata aataagagca
     7261 gttttagttg aactgctctt tttagatgtg tttaatttta tgtaagaata tgtattttta
     7321 ttaatcagct atatatgagc aacagtagtc aacaacttca ttaaccttta agtcaaaact
     7381 tgaatcacta ggaacattaa atttttgccc ttctttataa gttacaaact cattgcttcc
     7441 aggcagtttt acatccatac ttccagcaag catctccatt atttcttctt ctctagtaga
     7501 aaaagtgtat tctcctggca acattaatcc taaagttttt ctttctccat taggcagtat
     7561 tattactctg ctacttacct taccatcaaa ataaacgttt gccttcttta ctgcagtaac
     7621 attcttaaat tcactcatta gataaaacct ccaaaattat ttgtacatat atattatata
     7681 aaaataatgt aaaaaatgct atatatttac aaaaaaattt agtatttatt aaaaaaatta
     7741 ataagaataa aaaagagatg aaattgctta aattgataaa aaatataaaa taaattgcaa
     7801 agtaagcaag aaaagaataa gttgaagagt tatatattat aaatattaaa aattttagta
     7861 ttaataaaaa agatattaca ttaatatgaa aatataggag attagaagtg tttgataatt
     7921 acagtacatg ttagatatat cattgtaaat taacatagct ttgaatataa taatgttcag
     7981 aaggcaatat ttttgaggtc tgaatccaat aataatatag tcttatgatt gtagatatca
     8041 aaaaatgtaa gttactagga atacgttttg tagatttata tatgattaat aactaaagct
     8101 ataaataaac aaaataaaaa gagtatgttt caacctatag cttttagatt taaaatatac
     8161 tcttttat
//
LOCUS       DAELTA010000159         7932 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.160, whole genome shotgun sequence.
ACCESSION   DAELTA010000159 DAELTA010000000
VERSION     DAELTA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.160"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            90..458
                     /locus_tag="KPG13_003032"
     CDS             90..458
                     /locus_tag="KPG13_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG0986729.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            883..1128
                     /locus_tag="KPG13_003033"
     CDS             883..1128
                     /locus_tag="KPG13_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986730.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            1227..1490
                     /locus_tag="KPG13_003034"
     CDS             1227..1490
                     /locus_tag="KPG13_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG0986731.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(1775..2722)
                     /locus_tag="KPG13_003035"
     CDS             complement(1775..2722)
                     /locus_tag="KPG13_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG0986732.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(2725..4356)
                     /locus_tag="KPG13_003036"
     CDS             complement(2725..4356)
                     /locus_tag="KPG13_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0986733.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4486..5403
                     /locus_tag="KPG13_003037"
     CDS             4486..5403
                     /locus_tag="KPG13_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0986734.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(5460..6041)
                     /locus_tag="KPG13_003038"
     CDS             complement(5460..6041)
                     /locus_tag="KPG13_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0986735.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(6081..6647)
                     /locus_tag="KPG13_003039"
     CDS             complement(6081..6647)
                     /locus_tag="KPG13_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG0986736.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(7103..7927)
                     /locus_tag="KPG13_003040"
     CDS             complement(7103..7927)
                     /locus_tag="KPG13_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0986737.1"
                     /translation="MLILIVGCILMAISLNLFFNPHAIAAGGITGLGVVLNSLFGVEL
                     WIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMATLDITNDMYLAII
                     SGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIVDCVVVVLSGIVN
                     RQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKKIMEDLDRGATIL
                     KGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHEALGNGFKKIE"
BASE COUNT         2831 a         1200 c         1064 g         2837 t
ORIGIN      
        1 gatacaaaaa tagtatatat gatatgtaga ccattagcag gtttaaagta gcaaaacata
       61 taaaataaaa tttgaaagga agtataaaaa tgacaagacc atcaatatat catagccaag
      121 gatacacttg tgcagaggca ttaataaagt cttataatga ggaacataac acagatatac
      181 caatctcaat aggaagtggt atgggggttg gaatgaatgt tggaagtgtt tgtggagctg
      241 taaatgctgc tgttgttata atagggtatt taaatgggcg aaatagtaat ttagatgaaa
      301 atatagcaag gaagtattca agagagctat tgaatagagt aagaaataaa tacagctcag
      361 aaatatgtgt atctcttaaa aaaaataaag taacttgtga agaaataata aatttcacat
      421 atgatgctct aaatgatatg ctaaaaaatc aaaaataatt ctaagaaaca agtattttac
      481 attcgataga atacttgttt ttttatgata tttttaataa gtattagttt attataattt
      541 ttaaatcaat tttattatat tttaattgaa acaatctact ttgaaaatta ctaactgaca
      601 aaagaatatg attatagtat agaaaaaatg taaattattt aatattaagt gagagtatat
      661 gtttacctat ataaaatcgt ataaattaat ggctaaattt ataaaatata aagaaatata
      721 taggaaaaca aaagaattaa ttagtataat ataaagttta aaagttaaaa tttatataat
      781 ttatttataa agtaaagaaa aaatagaaat ttgtaataaa ataaaaagtt tatgacataa
      841 tatggtataa ttagtaaaaa tatagagatt ggaggaattg ctttgtttat agatgaagaa
      901 ttagaaggtt atatattaac atgtaaaata tctgaagact ttaaaaatat acctgaatat
      961 agtgatgaag agttttatgt tacagtctat aaagatgaaa gttctgactc tgggtactat
     1021 gctttattag aaaataaaga agaaagagtt gtatgggatg gagaagttgt tgccaataat
     1081 atttttaata acctttggat tgtagtaaat aaggttaaaa ctggataata gtctaattat
     1141 atagtatttg taatatttta aatgaatttt tacatatttg agatgagtaa ataacaaaga
     1201 ttattcgata ctggaggagt aattcaatgg aatatagtta tagtaagatg aacttaaaaa
     1261 aaggggatat tgtagaggta aatttagaga aacaagccaa tgtaatattg ttagaccata
     1321 taaattatgt gaaatttaaa aatcaaagaa attatgatta ctatggtgga tttgcaaaga
     1381 aaaatccatg tagaatgaga gttcctaata ctggaacatg gtatctagta gtaaatcaag
     1441 atggaaatag tggaatagta aacttttcaa tcaatacaat tcaaaattaa ttttagaata
     1501 ttggatattc ttaatttttg tacattttat atagttaaaa taaaattaat aagcaattat
     1561 tataaaataa tgtgggtgcc tcaaaatgaa ttttataatt catgagacac tcttttttat
     1621 atgaactcaa atatacttta tcatttcaac aatgaaaaag aggcgtatct gtgttttgag
     1681 atagcctcat ttatcatata tcatatatag aattttaatt attcttaact aaaatttttt
     1741 ccataggtta tctgtacaat taaaatattt attgttagca tacagctcca cctgtaaggt
     1801 gtaaaagagc ctctagtatc tcttcttttc tttccaaaac ttcatctgat aaaagcattt
     1861 ctataaattt atcagcaccg attctatcga tagttattcc aaaacgttca cctgtgttac
     1921 cctgttctct aaataaaaga attgcttttt caattaaagt cataagttcc tcttttgaaa
     1981 ataatctatc aatctgtgtt ccaggacgaa ctgattttcc ccatttccct cctatataga
     2041 ctttataacc agattctttt tcttcaatac tatcaaaatt acaactttct atacacttac
     2101 cacagttatt acatagatta ctatctattt caagtttacc tttatctgtt aatttagctg
     2161 ctttaacagg acagacctct acaacagcac attttttaca accaacacaa agctctgaat
     2221 catagtcagg tacacgttga ccaacaattc caagatcgtt taggtcaggt ttaatacagt
     2281 tattagggca tccaccaaca ccaattttga acttatgagg tagttttaca tcataccaat
     2341 ttttgtaaaa ttcttcatgt atttcagcag caagtgcctg tgtattagct agaccatgaa
     2401 cacaaacagt acctttacaa gccacaacag gacgaaccct tgatccagtt ccacctgtta
     2461 ttagattttc gctagcaata aattcactta gagcttcaat atcttcaaat tttacaccag
     2521 gtaattctac tgttagtcta gtagtaaatg ctaactgacc gtttccgaat ttttcagcag
     2581 cttctgcaat tttttttgct tgtgatgcat ttattacacc atttacagtt attatacgtg
     2641 ctgcaaaatg ttctccatcc ctgcttggta gaaaacctct accttttact tccttttttt
     2701 gttctgctgt aacattactc atatttactc ctccattacc ttaagatttg gataaaatag
     2761 tattccacct tcaagaactt tcgtatttgt atagccaaag cgtcttaagc gattttgcgc
     2821 catatagcta tttttacctt tagcacatat aagaagaatt ttttcatcct tagcaagacc
     2881 ttcaatttct ccattaattt caccaacagg aatataacgt aaactaggaa tagaaggagc
     2941 ttttgaaaca tcaagtttag tccaactgtc aaaatcctca atttcgtcaa gtaaaacact
     3001 atcaagttct ccattaagtt tattttgtaa aacatttact gccactacaa aagggtgaat
     3061 agctgttgaa aaaggtggtg catatgcaag gtccatactt tctaatgaat aaaggtcagc
     3121 gcctaaagtt attgcagtag cagcgatatc tactatttta tcaaccgctc caggtcccat
     3181 aacttgaaca cctaataatt ttctagtatt tttttcagca accattctta taataaaatt
     3241 agatgcacct ggataataat gagctttatc atcagtagct atggttacag agcaaacatt
     3301 ataaccttct ttttcagcta tttctttact tagacctgtt tttcctgcat tgagctcagg
     3361 taattttact actgttgttc caagaacacc attgtaagtt ttagattttc cagatatatt
     3421 ttgtgcgcaa attctaccct catgatttgc agatgacccc ataggtgacc atgtaggttt
     3481 atttgttaaa gaatttttta caaaagcaca gtctccaact acatatatat ctttgtcatt
     3541 tgtaagcata tattcatctg caataattgt attattaggg gcaaattcta aacctgtgtc
     3601 ttttagaaaa cctgtgttag gacggattcc aactgacata accactaaat ctgttttaat
     3661 agctctattt ttagtacgaa gtttttcaac tttattttca ccttcaatac ctacaacctg
     3721 gtcacctgta aaaatcatta tgcctttatc agccatatga ttttcaacgt attcagcaaa
     3781 atcagtatca aatccaggca taacatgttc agccatatca acaacactta cacgtatacc
     3841 cattgcagaa agattttcag caatttcaag accaatgaaa cctccaccaa taacaacagc
     3901 tctcttaata ccgttttcaa tagaatctct tagttttata gcatcatttg gagtacgcat
     3961 aaagaatact ccaggtaaat ctattccagg tattggtggt tttattgggt ctgcccctgt
     4021 agcaattaca agtttgtcat agcttagatt ttctttttct cctgttgaaa gcgttttcaa
     4081 agttacttgt tttgcatttc tatcaataga tgtaacttca actccacaac gaacttctgc
     4141 tcctgttagt ttagaaaaac tttcaggtgt atttacgatt aaagatgatt tatcttcaat
     4201 tatcttacca acgtaatatg gaagaccaca acctgcataa gaaatatcat cacctttatt
     4261 tagtattgta acagagcaat tatcacccat ttcacgtttt atttttgcag cagtttttgt
     4321 tccagcagca actccaccta taattaatac tttcacaata tatcacactc caatctactt
     4381 tataactcta taataaaatt atacttcttg acaatatatt tgtaaaatac taaaatatta
     4441 ataaaatcat aagcatttac taatgtttgc taaggttgtg ataatatgac aattcgtagt
     4501 cttgaaatct ttgttaaggt tgctgaatgt ggtaaaatga gtgaagtagc aagaaatatg
     4561 tatattaccc agtcatcagt tagccaagct atttcagaaa tagaaaaaga gtatggtgtt
     4621 aagctatttg atagaatttc aaaaaaatta tatttaactg aggcagggaa aaaacttctt
     4681 ggatatggga ggcatctttt agctgtaaat gaagaaatga atgattgtat gaagcattgt
     4741 gcaaaaaata tccgtataag agttggggca acagtaactg ttggtacatg tgtaataagt
     4801 ccaataatgt tagaattata caaggtaaat cctcttatag aacctgaagt atttgtagaa
     4861 gatacaagac tgattgcaaa aaaattactt aatagtgaat tagatattgc gattgtagaa
     4921 ggaaaaatta agcacccaga tattgttaca aaaagcatta ttaatgataa tcttgtactt
     4981 atatgttcac acaagcatga gttttataaa agagactcta taaaagtttc agaactttca
     5041 aatcaaccat taattatgcg tgaattggga agtggtacaa gagcacaatt agaaaagcaa
     5101 ttaaaagaat taaaaatccc aatgaatatt agatggtcat gttataactc tgaagctata
     5161 ttaagagcag ttgtagataa ttttggcatt gcagttatat ccgaattact tattgaggat
     5221 tatttaaaaa agcatctttt gtgggcttgt gatattgaag gtattaattt acataggaca
     5281 tttgatattg catatcatag aagcaagttt tttacagaaa atatttcagc attttttgat
     5341 atttcagtag aatatgggaa aaaacaagcg agaaagaaga gtagaagtgt aaacagttta
     5401 taatatatct aaataaaaat aagagtatat caatatactc ttatttttat ttagatatat
     5461 tagcctatat taacgccaaa atttttacac aatcctgcaa gtccatcctc aaatccagaa
     5521 cctaaagcat taaatttcca ctctccatta tgtctatata gttctgccac aactattgct
     5581 gtttctatgc taaaatcttc acccaattca tatttaataa gttcctcatt agtttcttca
     5641 ttatatattc ttatatatga attttcaact tgtccgaaat tttgtcttct tgtaattgct
     5701 tcatttatag taacactaaa tgatatttta gcaatatttt gaggtatttt agataagtct
     5761 acatttattt gttcatcatc accatcacca acacctgttc tattatcacc catatattca
     5821 acagctccag aagcgtgttt taaattatta tagaatataa agtctccatc attcgttact
     5881 tttccatcag ttccagttaa aaatgcagag gcatctaaat cgaaatcaaa gccaccatca
     5941 tatttattaa tatcccatcc tagtcctact attacttttt ttagaccagg gttacttttt
     6001 gttaaactta ctttttgtcc ttttgctaaa gttataccca tttaaaccaa ctcctcatta
     6061 taaatttttt attgtaatta ttacttatat gtattaatta attcacgaag ctcttcattt
     6121 tgaccaattc ctaaagcatt aaatttccat tcttcatttc tacgataaat ttctcctgcc
     6181 aaaactgtta ctttaccaga aaagtcttct tgaagattat atttaaataa ttcttgcttg
     6241 gaatttgaat caattaatct tataaaagag ttgtttattt gaccaaaatc ctggtctctt
     6301 tttttagctt tgtatataga aactccaaaa actattttat gtacatattc aggtatgtct
     6361 tctaaattaa caattatttg ttcatcatca ccttcacctt ttccagttaa gttatctcca
     6421 gtgtgtataa tagctttaga agggtgttta agatttccaa aataaaccaa gtcacctcta
     6481 acattcattt tatcttggtt agtcaatgct attgcaaatg catctaggtc ataatttttt
     6541 ttacccaatt tattcatatc ccatcctaag cctactacta gattttttac aggattatta
     6601 tcttttacta agctaactct ttgtcctttt tgtaaactaa cactcatttt aagttctcct
     6661 ttttatattt ttagttttat tgatggtatc aagtataaga accatattag ctatattata
     6721 tacgtattta ggctaagttg gctatattta taaattattt attatataac tacatgaata
     6781 ttttataatt taaacatatt gacattatat gctttaaatt attttgtagt tttataatat
     6841 ttaaatttgt atttgattgt tataaagtaa agtaagtaat aaagttagac tcaataattc
     6901 taaaatttct aagcatacta tctaaatgag aacggatttg gaaatacttg tataaaacaa
     6961 taattgatac atttaatttt tatcttaaaa acaacaatta taatatataa atagcaagta
     7021 taatatataa atagcaagta taatataaca tataaaaagt tgaatttgta tatgttaatt
     7081 atacttgcta tttatttgtt agttattcta tttttttaaa tccattacct aaagcttcat
     7141 gtatatctgt aattataata aaagcattag ggtcgacatt ttttactaat tttttcaatt
     7201 cgacaacttc tttcttttca actactacta aaagtacatt tttattttct ccagtatatg
     7261 caccttctcc ttttagaata gttgcacctc tatctaaatc ttccattatt tttttactta
     7321 tatcttttga aaggtcagat ataatagtga atgatttgga ataattaaat ccttcaataa
     7381 gcaagtcact aacttttact aaaatgtata aagccacagt tgaataaagt gcaatttcaa
     7441 cctgtctatt tactatacct gataaaacaa ctacaacgca gtctacaata cccattaaaa
     7501 caggtatact aaatgtagga aaatatttat ttagtaggag tgccattaaa tcagttccac
     7561 ctgtagagcc atttattcta aaaataagtc cttgaccaac tcccattaaa atagaaccag
     7621 atataattgc aagatacata tcatttgtta tatctaatgt agccatattt gcagtaagct
     7681 taagtgctat ggtaagaaaa attattccta aaaccgtttt aaaacaatct ttttttgaaa
     7741 gtattttgta agcaaatatg aataatggaa catttaaaag taaattgaca atccaaagtt
     7801 ctacaccgaa taatgaattt aaaacaactc caagtcctgt tataccacca gcagcgattg
     7861 catgtggatt aaaaaataag ttaagagaaa tggccattaa tatacagcct acaattaata
     7921 ttaaaatttc tg
//
LOCUS       DAELTA010000160         7751 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.161, whole genome shotgun sequence.
ACCESSION   DAELTA010000160 DAELTA010000000
VERSION     DAELTA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.161"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(372..707)
                     /locus_tag="KPG13_003041"
                     /pseudo
     CDS             complement(372..707)
                     /locus_tag="KPG13_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(805..1485)
                     /locus_tag="KPG13_003042"
     CDS             complement(805..1485)
                     /locus_tag="KPG13_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986738.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(1482..2408)
                     /locus_tag="KPG13_003043"
     CDS             complement(1482..2408)
                     /locus_tag="KPG13_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986739.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(2481..3398)
                     /locus_tag="KPG13_003044"
     CDS             complement(2481..3398)
                     /locus_tag="KPG13_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0986740.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(3398..4087)
                     /locus_tag="KPG13_003045"
     CDS             complement(3398..4087)
                     /locus_tag="KPG13_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986741.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(4495..4800)
                     /locus_tag="KPG13_003046"
     CDS             complement(4495..4800)
                     /locus_tag="KPG13_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986742.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(5957..6175)
                     /locus_tag="KPG13_003047"
     CDS             complement(5957..6175)
                     /locus_tag="KPG13_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986743.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(6176..6583)
                     /locus_tag="KPG13_003048"
     CDS             complement(6176..6583)
                     /locus_tag="KPG13_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986744.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(6669..7157)
                     /locus_tag="KPG13_003049"
     CDS             complement(6669..7157)
                     /locus_tag="KPG13_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG0986745.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(7362..>7751)
                     /locus_tag="KPG13_003050"
     CDS             complement(7362..>7751)
                     /locus_tag="KPG13_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986746.1"
                     /translation="LPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKRALD
                     RLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIIIKLKN
                     KGYNLSEICDIIDDLNKSEVEKVYNQN"
BASE COUNT         2651 a         1228 c          746 g         3126 t
ORIGIN      
        1 taaaattttt tattttttat tttcttatat tatcaatttg aaatatgaaa gattaagact
       61 aatagaattg attcagttaa ttgaaaaaaa taggtacctt ttatgaaaat tctaatgata
      121 agcatttttc tgatgtaatg gtaaattata aatgataaaa ttaaaaatca cttttagttt
      181 tatgaactgt ccccatatta agaaacaact aagatacata aaaatagtta taaagagata
      241 gttaaactat atccactatt ttatatagtt aaaatagtgg gtatagtcca agctaagaat
      301 aactaagaaa ttagcgttaa atagcataga taaaaataca tttatatata aacttttttt
      361 gctgcaaaca attatttcca taacatttta tatacttctc taaaatcttc taaacttgat
      421 aaatattcaa ttaaagggac ataattacct aaactacaag aatttaagga gtttctatac
      481 aattcctcac tatcccaaga tacaattttt ataggaagat tattttctaa catctgtttt
      541 agcataacaa atctacctat gtgaccatta ccatcttgaa atggatgtat caattcaaat
      601 ctataatgaa attcagttat aactttaata gatacttcac tctgactata gtaccattca
      661 attaattcat caagctttgg ctcaacttca aatggttgtg caatattatt aaaactctaa
      721 taatattaat aatagctgat ataatagcaa atcattatta tcccgagaag atacaattaa
      781 taactacctt cctatccaca gtatctagtc aatttcagta actttatgat ttagtatata
      841 atatatacca atcacagtta taccaactat aataaaaaca gaaatcaaac tgtcttgagt
      901 tgtatttgta ccagctaaaa taaattgtct taaattgata aaaataattg aactgacaag
      961 ggtaacaggt attgattttc ttatcattcc aacaacgaat gtccatatac ctaatgagca
     1021 aaatacaata atacttttaa ttgctcctgg tatccaataa gataaaattg aaatatgaaa
     1081 atctccaata actaaattaa aatacttatc caacacaaca acgaatgtaa aacaacagat
     1141 atatccaact atcattgatg atatcataag agtcgtaatc aaaagtaact ttgataaaat
     1201 aagtttttta cggtcaagtg gataagagaa taaaatcaag attgttttat ttgtatattc
     1261 atttattata tatgccacat tcaacacagc aaataatata atatagtaaa aacagaaaaa
     1321 taaactgatt gtcctaaatg taacatcata atcatatatt gaagtagatt tatcattcaa
     1381 tccagtaaag acaaaaaaca tacataagaa aatccccagc attgaaaata aaaaatatct
     1441 ttttagccca acttttttta gctctagttt cattaaatgt atcatagtgt ttctttacct
     1501 cctgtacctg tagtttggaa gaagtaatct tctaaagaac tttgtttacg tccaatgcta
     1561 tttataccaa tttgattttg aataaatatt ttggatattt cttttacatc ttttcgtaaa
     1621 tcatatattt caatacaact ttccgaagta attttaaagt cttttattcc aaattctttt
     1681 tctaacaagt atccagcaag ctccacatta tctacatcta cttcaacata atctgtttga
     1741 tatttgtgta tatctgacat agaaatttct ttaataagtt taccatcttg aataattcca
     1801 atagtgtccg caatttgttc tacctctgaa agaatatgac tagaaataaa tatagttatt
     1861 ccataatctg tatttaactt acggaataat tctcgcattt ctcttatccc ctctgggtcg
     1921 agtgcattta taggttcatc aagaatcaat aattctggtt tagtaagaat tgctctagct
     1981 attgcaagac gttgtttcat ccctaaagaa tattttgaaa ctttcttgtt tgattgcttt
     2041 gacaatccga cacattccaa tacctctgat atatgttctt tgttataata acccatatat
     2101 tcacagtgta aatcaagatt ctgtttagca gtcaaattct catataaata cggattttca
     2161 ataatagtac caatcttttt taatatttca aaatcagttt ctgccacttt cttaccgaac
     2221 ataactattt ctccagaatt tggcttaaca agatttagca acattttcat tactgtactt
     2281 ttacctgccc cgtttggtcc aacaaatcca taaatttctc cctttttcac atgaatatta
     2341 acagcattaa caacagcttt atctttatat attttcttta attgattggt ctctatgata
     2401 tagttcatca tactccctcc tttctaaaaa cagtatacaa aataaaatta tcttttctct
     2461 cttttaaatc taactttttt ctaacttttc aattttactg taaaaactgt ttttacatta
     2521 ggtctactat ttacataaat tgaacctccc atagagttta caagcccttg agaaatagcc
     2581 aaacctaaac cattgccttt ttttctgtca gcagtataag ctcttgtgaa aatatgctct
     2641 ttttcagttt cagaaatacc tgaaccttta tcctcaatat ctataaaaac atgtttttcc
     2701 ttttcatata gtgaaatccc taaaaatcta ccatctgaac catattttat agcattatca
     2761 attaaatttt tgagtatacg ttgtaatgct tctgtgtcaa catttgcata tataggtcta
     2821 tcttcaatat taacttcaac attaaagttt tctttatcta gcaaatcata aaactctaaa
     2881 ataatctcat gacataattg tgttagattg gatttttgaa gatttaatat catatctcca
     2941 gattggattt ttgccatatt aaacaggtta tttattgaat taacaagctc atttgaattt
     3001 gtttgcattt tttccacagt tgctctcatt gaagaagata attcttcttt ttgactttga
     3061 agatataaca tttcaatata accctttaat actgtgatgg gagttcgtaa atcgtgtgat
     3121 atgtttgtaa atatttgcaa catcgctttt tcacttctat tataattaat ttgcctgctg
     3181 taaaactttt caagaagttt atttatatct actgctgttt cttttacaat cgaaatatca
     3241 gatggtacta aaatataatt aatttcatca tcatttgtta tgtcacgaag tcttgaattg
     3301 atatacttat aatcactttc cattttcttt gatttatttc gttcttttaa gtagagagca
     3361 aatagtacaa tcgaaattac aactaaaaat aactccatta ttcttcctcc attttgtagc
     3421 caattcccca aagagtttta atataattat ttttttctat accatcagac aatttatttc
     3481 ttaacctgtt aatatgagta tttagcacat tttcatttcc ataataaggt tctttccaaa
     3541 tagcgttgta taattgttcc tttgaaaaag cacgtccttg attagtcgct agtaatttaa
     3601 caatttcaaa ttctgtatgt gttaagttta agtcaattcc attctttgaa actacatatt
     3661 ttgatatatt tataatcaaa tctttatact gaataaccat atcttcagag tttcttgaac
     3721 tgctgtacac tgttgcacgt ctaatattag ctttaattct tgcaagcaat tctacgattg
     3781 aaaaaggctt tgtaatataa tcatctgcac caagattaag ccctagcatt ttatctgtgt
     3841 cactatcttt cgcagtagta ataataacag gaacaacact cacacttcgt atatatttta
     3901 atacttcaaa accactagac tttggtaaca tcaagtccag taaaataata tcaaatgaat
     3961 aatttttaaa tatttcaata gctgtatttc catcaatggc agttttaaca gagtaattct
     4021 cactaatcag aaaatcgtat accatttctc gaatattctt atcatcttca accaataaga
     4081 tgtccattat tattcaactc cttttcgtca gtatacttca aatgatatat ttattaaaaa
     4141 gggttgtcaa taaataactg cttatgatat tgtatctgca atttggtgat actcttctaa
     4201 gttaaagttc ttttcaataa gctttattga aaaaataatt aaaatatagt atactctctt
     4261 atctttatct gaaatataca taattttctt ttattaacat ctacaattaa ctattttata
     4321 ttataaaaca ccttttaact cttgctaaaa aatattttat acataaaaaa gaaaccacat
     4381 tctagtttta tacagaacat agtttcttta aaaataatca aatttggatt ttaaataatt
     4441 aaaaacaaac ttttatttgt ttttaatttt ccaattttat agtagataat ttagttaatt
     4501 agttcttaaa tgtttatctt ctttagtaaa tttttttctt attaagaaat atgcaactat
     4561 tacaacaagt tctattgcta aaaatacacc ataagtagct atagaatcta tgctatctat
     4621 gccatttcta aagaaaatta aagcaattcc tgctgctata atacctacag catttttccc
     4681 tatagaattt tgaccaaaac cataaaaaga agggaaattt ccttttattt caatgaatgt
     4741 caagattaat ccaatagcta taaatagtac tcctacaact tccataatca tgctaggcat
     4801 tttgacccct ccttattaat aattaactac tactataaaa atatttatta tttttataat
     4861 tatattatat atgcgattac atgtttttgg aagtatattt ggaagtaatt tcatatttat
     4921 ttaacttaat ttttttgtga taaaatactt ctaattttta tatagatatt attataaatt
     4981 ttctagttta tggagtcatt cattctataa ttcacaaata tgtatttttt aatctattaa
     5041 agtattcatt ttctggctta taagcatgga cttcttatct ataagcttgg tttttcatct
     5101 aataagttat ttattcttta atctatgagc ttgatttttc atctataagt catttatttt
     5161 ttaatctatg agtttggttt ttcatctata aataagtcat tttttaatct ataagtttag
     5221 ttcttcatct ataaatcatt tattttttaa tctataagtt tggtttttca tctataaata
     5281 agtcatttat tttttaatct ataagtttag ttcttcatct ataaatcatt tattttttaa
     5341 tctatgagct tgatttttat ctataattaa ttcattctct aatctattag cttggttttt
     5401 atttataagt catttattct ctaatctatt agcttggttt ttacctataa ttaattcatt
     5461 ctctaatcta ttagcttggt ttttatttat aagtcattta ttctctaatc tataagctta
     5521 cttcttaatc tataaatcat tcatttttta atctattagc ttggttttta cctataaatc
     5581 atttattctc taatctataa gctttctttt tcatctataa atcattcatt ctttaatcca
     5641 ttagcttggt ttttcatcta tgagtcattt attctctaat ctattagctt ggtttttatt
     5701 tataagtcat ttattctcta atctattagc ttacttctta atctataaat cattcatttt
     5761 ttaatctatt agcttggttt ttacctataa atcatttatt ctctaatcta taagctttct
     5821 ttttcatcta taaatcattc attctttaat ctatgagctt ggtttttcat ctataagtca
     5881 tttattctct aatctataag cttggttttt tacctataat taattcattc tctaatgcat
     5941 aagtaataca tgaaatttaa atattatttt cttcaataaa ttcaaacaac tcttctacag
     6001 ttgtatcaaa taccttagca atatccattg ccagttttaa agaaggattg tagcgaccat
     6061 tttctaaatg tacaatagtt tcacgtctta ctcctactga ctttgcaagt tcaccttgtg
     6121 tcataaggtt cttttctcta taggatttaa tttttgtctt tagtaatact gacacctaat
     6181 tgtatatccc ctttctgtca aaatatataa atagaattaa cttcaacaag gattgaataa
     6241 acaaaatgac taataaagac attcctatat ttaaatttga cacatttatg tccaaagtta
     6301 caagaaatat agatactata gctacagaaa aaaacaatac ttttattgca atatcattaa
     6361 ctcttgatag tatacatttt gatgcctcat caattaaatc atcatacttt ttattataga
     6421 cccactgagc tcccatagct aatacccaaa taattatagg aagatattca atactacttc
     6481 ctttaaaaaa atacttattt actaatgtag ctccaataca ataagaaatt cccaatattt
     6541 ctgaaataag tgttgattta ataccagttt gttgtttaga catataaaat cccccttaaa
     6601 aagttatgta tttataacac ctcttgttat aaatatataa cttttttgaa taaatttcaa
     6661 taaaaatatt attttgcaac aaattctttt attaatttta cattagatag aggcacttta
     6721 gatgtcactc cttgcaatgt caaatcttct atattaactt catttaattt tgaacacagt
     6781 tctaagtcaa atattctttc ccagcttacc ttcttttcaa tttgttcatc ttcataaagc
     6841 tctacagtaa tatccattaa aggtaaatgc cataaatcaa attctgataa taacacattg
     6901 tcttcactta tttctatttc aagtaaaaca cctttttttc ctttttctaa ataaccacta
     6961 catcttaaat caggtctctt cgtccaaacc catatcatac tatcatcaaa ttcatatatt
     7021 ctatttgtca tttgactata catccatttg tatggaatat caaagtcttc taatataaaa
     7081 gaataatcat ttgatagata tttattgttt ttattgttat tccaaaattc taaagtctgt
     7141 atagtataaa atttcatata taaatcaacc cctttgtttt tctataagaa ggagatggtt
     7201 caaactttta ttttgaatta tctcctttat ttatacacct ctttgtacct attatttata
     7261 tatctcttta tatctaccat atttaaataa caaagtttta tattaattta aatttaaaag
     7321 aggaagcttt gtatttatct agtgccatct caatcatagt attagttttg attatagact
     7381 ttttcaacct ctgatttatt caaatcatct ataatatcgc atatttcact caaattgtat
     7441 cctttatttt ttaattttat gatgatatta aatttctctt ctcttttccc ttttgcaata
     7501 cctttttcaa taccttcttg aaaaatttct tctttaaaat ttctactttt attcataata
     7561 tacgcacctc tctttaaatt taataaatag tcctcattac tacctaattc ttttaatcta
     7621 tcaagtgctc ttttaataac ataatccgtt ttagatagta ttctaatatc tatatgagaa
     7681 ggattatcaa taagtttacc ccatattaga actctgtttg ctatacaact ggatttacga
     7741 atttttggaa g
//
LOCUS       DAELTA010000161         7698 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.162, whole genome shotgun sequence.
ACCESSION   DAELTA010000161 DAELTA010000000
VERSION     DAELTA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.162"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(198..2477)
                     /locus_tag="KPG13_003051"
     CDS             complement(198..2477)
                     /locus_tag="KPG13_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG0986747.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2748..4202)
                     /locus_tag="KPG13_003052"
     CDS             complement(2748..4202)
                     /locus_tag="KPG13_003052"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG0986748.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4366..5235
                     /locus_tag="KPG13_003053"
     CDS             4366..5235
                     /locus_tag="KPG13_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986749.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5448..7520)
                     /locus_tag="KPG13_003054"
     CDS             complement(5448..7520)
                     /locus_tag="KPG13_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0986750.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2609 a         1282 c          821 g         2986 t
ORIGIN      
        1 tcccatatac tcttacctca aaaacattca aatgtaaaaa atactacata ttaataaact
       61 ttgcccaata attcctaaaa taatatccta acaatgattt atgtttttat aatagtatat
      121 ttttacataa aaaagtgaac attgattaac aaagttcact tttttataaa tttttatgtt
      181 ataattcaca attttattta atttgtttct tttctattta aactatatct atctaatatt
      241 aatttttcat attcttctat tggcattggt ctagcaaata aaaatccttg ggccatatca
      301 caacctataa accttaaaaa ctctgcttgc tctacagttt caacaccttc tgaaacaact
      361 tttatatcaa gttgctttgc catttgaaca atacttgaaa ctacaatttg actctttttt
      421 gcatttccca ctgttataaa gaattgccta tctaatttca atacatcaat tggcatatct
      481 ttaagcatat ttaaagaaga ataaccagaa ccaaaatcat ccatagaaat tttaaatcca
      541 acagatttca aagtttgcat tattttaaat aatatttttg tattgtcaaa tattgcactt
      601 tctgtcaatt caatctcaat taaatttggt tcaatttcat attcttttgt tattctttca
      661 agctcagaaa taaagcctgg attctcaaga tgtactcttg ataagtttat tgatatagga
      721 aatgtgggga aattttcatt ttcccaacgt ttaaaatttt tacaaacttg ggtaaataca
      781 aacatatcca attctgttat aaaaccattt ttctcaaaaa taggaataaa tacacctgga
      841 cttattagac cttttttggg atgttgccaa cgtacaagtg cttcagcacc tacaatttta
      901 gtagttgata aatcaacctt aggctgtaaa taaactttaa attgttcatt ttttaaagca
      961 tcatgcattt cttgttcaat atcacgttct tctatgagag tttcttttaa attttcagca
     1021 taaatctcat aaacttttaa atattttctt tttactgtag tttttgcaat catagctttg
     1081 tcaagacagg tagaaatatt tgtttctccc tcttcaacaa aatatacacc tatggatgga
     1141 attaaattga gttctactcc caaacttgtt tttatcattg acaatttttt acgtataagt
     1201 tcacatattt ctataattga ctctctatta tttttctttt caaatattat agcaaaattg
     1261 tcatttgaaa ttcttgaaaa tacagattgc tctttaaata tattcttaat tatctctgct
     1321 atttccttta atactctatc tccttcttca tacccaaacc tatcatttac tagcttaaac
     1381 ttatctatat caaaataaat taaaactaga ttctttttct catgagagga taaaaatttt
     1441 cccccttcaa gattaaattt atttgaattg ccaatacctg taacgttatc tttaaaagca
     1501 aattcttcca atttttcctt actcttttta tgtataaata gtatataaat tgttaatgcc
     1561 gtaaagatta taatcaagac tacacatgct gataatgtaa gttttataat agcatatgat
     1621 ttctcagata tagcagtttt aggaacttca gaaaaaagat accaatcatt gattcctatt
     1681 ggggcataac ttaaataata tccttttcca aacatactat aacttaatga acctgattta
     1741 aagtttttta tatttgactt catattgttg atactagatt ttgatattga atcacttctg
     1801 ccaatgtaag aaaataaatt ttctttagta gagttggcgt ttttactgct tggatttaaa
     1861 acaatatcac cattacttct agttatataa gaatatcctt ttccattaaa tgtaggtaca
     1921 gaaagtattt tttcatacaa cttcgtacta tgtgtggcaa atataactcc tttaacctca
     1981 ttatctttat aaattggtac agaatataca ttaatattac tattggtatt tttatcatct
     2041 tctattacat cttttaattt tcctgttata accacttctc cttgcattga ttttaagaaa
     2101 taatctcttt tagagaagtc ttctatataa ccatctgtag tgtatgctgt accatttggt
     2161 aaaataactc ccattctttt aaatgaacta ttaatagctt gtctttttag tatagaaagg
     2221 atattatcta cttcaaaaga ctcttctcct tcaataaata ttgaaattga ctctagtaac
     2281 tttatatctc cattaatttg tttttttaac acattgacgc tttgatttgc tatttctgaa
     2341 agatattgat tcgtttcttc acctaatgtc ttattcaacc ttgaaccata aatcaaacag
     2401 gaaattgtaa ttactaagat acttatgaca gcaattatgg acaaaccata cctattattt
     2461 tttgcatcac ttaacattat gcttcctgcc tttctcgaat ctatgtatgt ataatcatta
     2521 tcttatgaca attattgtaa acattttata ataatactta tttttccata tttactgtaa
     2581 ttatacaaaa ttataaactt tattacaaca ataaattaat taatatagat attagtgttt
     2641 acattattgc attatctatt ttacaattat ttacatataa aaaaatgtaa tatcaaagtg
     2701 aagcttgata ttacattttt gttcttgtat tttgtttaat ttttatgcta catcttactt
     2761 aaacactcat ttgcaacttc tttatcatca aaatgatgct taactttacc tataatttga
     2821 taagtttcat gtcccttacc tgttataatt acaacatcat ccttttttgc catacttata
     2881 gcttctgcta tagcttgttc tctatcaaca acaacatggt aattttcttt tttcttgtca
     2941 attccttcaa gtatatcttt tattattgcc tctggttctt cagttcttgg attatcagaa
     3001 gttataatac ataaatcaga atatttttgt ccaattgctc ccatcaaagg tctcttttca
     3061 gtatctctat ctccaccaca cccaaacaca gaaattattt ttccttctgc aaattgttga
     3121 gttgtcttta gtacattttc aagtgcgtca ggagtatgag catagtctac aattacgctt
     3181 atacctttat catttggaac tgtttcaaac cttcctgata ctccattaga aagtctaagg
     3241 ccttctttat atattggttt aggtatgcca agtacatagc aagcagctat aactgctaaa
     3301 gtattatata ctgtaaacat ccctggaact ggtataaata ttacttcttc atatgaaggt
     3361 gttattaatc tataagatac tccatccgaa tctgatttta tatctctagc cataaaatca
     3421 gccttagtat caacaccata agtgtaacaa ggtacattta taccttttat attttcatat
     3481 atcttttttc caccttcatc atctatgttt attatatttg ccatagtagt cttaaaaaat
     3541 aatttttctt ttgcttttct ataatcctct aaatctttat gaaaatctaa atggtctggt
     3601 gttaaatttg taaatatgcc tagtttaaag tctgtttcat caactctatt taatgccaat
     3661 gaatgtgatg aaacttccat tgcacagtag tcacatccat tatccaacat attattaaaa
     3721 tgatattgta aatctatact ttctggagtt gttgaattgg aaacaatgtc tttttctcca
     3781 tcaaaaattt ttatagttcc tataagtcca actttattct tatttaaagt taatatctca
     3841 tttagtatag tagttatact agtttttcca ttagtacctg taactcctat aacgttaaat
     3901 ttttgagatg gatgattgta aaaattatca gctacttttg ccatatcttt tcttgtatct
     3961 tttaccttta taaatgtaca tccttttatt gctacatctt cttcaacaag aaatgcactt
     4021 gctccctttt ctatggcatc ttttatatac ttatgaccat ctgacacaaa tccttttata
     4081 catataaata atgtaccttt tgtaaccttt cttgaatcat attgtacatt attaatatct
     4141 atatttaatt cacccttgac attgattata tccaacccct gtataatatc atttaatttc
     4201 ataaatgctt tcctcccttt aaactctttc tgctaaaatt acactacata taatattata
     4261 ttaagaattt tttactatgt gaatggttaa aaatgcttga aacattgcaa aaaatgctct
     4321 ataattttct ataatataac tgaggaaagg tcttctataa aaactatgga taataaaatc
     4381 gggtacttaa acgataactt taaaatattt aatattagag ataaaaaaga tatacaattt
     4441 gaataccata atcatgattt taataagata attatattta taaatggaaa tgtaacttat
     4501 tttgttgatg gtattcccta taaactaaag ccttgggata tactctttat cagcaataat
     4561 gaaatccata aaccagttat agattcaaat gtcttttatg agagaattgc aatatggatt
     4621 agcccagaat ttatgaaaaa aaatagtgat gctagtagtg acttagaaaa atgttttaaa
     4681 atagctacag agaataaatg caatagattg cgtttaaata tgcatgatat tgaatctatt
     4741 aagagtttta ttgagcaaat aaatatttcc gaatccaata ctgaatttgg aaatgaaata
     4801 ttgagaaata cattatttat acaacttatg atattcttaa acagaatttt cctaaaaaat
     4861 gaaaatatgg aatcaacaga agatgttttt tatgatgaaa ctattaagaa agtattaaac
     4921 tatataaatt caaatataga tgataagctt tcaatagata gcatatcgtc tgaatttttt
     4981 ataagtaagt attatcttat gagaaaattt aaatctcata ctggaacgtc tatacacaat
     5041 tatatagttc aaaaaagatt aattttggca aagtcactaa taaccaatgg tcaccttatg
     5101 agtgaagttt gtaataaatg tggattcaac gattattcta gttttgtaag ggcatttaaa
     5161 aaaaactacg gtgtatctcc taaaaattat ataaacaata aaaatccttt agatattact
     5221 cttttagatg aatagataat ttatgtaaat tttattttaa taaaacttga agtattagtg
     5281 tatttatctt actaatacct caagtttttt aatgttttaa aatatatcaa aatataatga
     5341 tatatatttc tattacttaa aatatatatt aaataaattg catgttaact gaacctattt
     5401 atatatttat taaatctcac ataactttta gttacactat catatagcta tcctattttt
     5461 tcaatatcat ctcttaatat acttactcta ttctcctctt ttgctgtggt tactcctttt
     5521 ttaatgctta atttctgatt ttgttctaaa ttcgtaaata ctacaggact tattgttgat
     5581 agaccatttt tttcaatata ttctaagtcc attttaacta atttttctcc cgcttttact
     5641 tcatctccta cattcacaaa tacttcaaat ccatttcctt ctaagtttac agtttctatc
     5701 cctaaatgta taagtatttc tactccactt ttttgggatt ttattattat tgcatgctta
     5761 gttggaaata taactccaat agttccatca actggtgaat aaatcaatcc atcactagat
     5821 ttaattgcaa atccatcccc catcagttta cttgaaaata cagaatctgg cacttcttct
     5881 atatctaata actctccatt tgttaatgaa tcaatctttt ctactaaagt attttccata
     5941 tttgcaactt ccatattttt tttcacttta tttttatcaa catttttagt aacagtactt
     6001 tcaacctttg tttttccctc tattatatct tttatatcag actttattga atctgatttt
     6061 ggtccaaata tagcttgaac attgttgcca ataaccataa cacctgctgc tccaagtgat
     6121 ttcaattttt ctttatctac tttatttata tcatttacaa ccaatctaag tcttgttata
     6181 cacgcatcta gactctttaa gttgtcttta tttcctaaag caatcaaaat ctctgatgct
     6241 aattcattct tagacatttc aatgacttca gcgttagttt ctgactcatc ttctcttcct
     6301 ggtgtcttta aattaaattt tcttatcata aatctaaata cactatagta taatactgca
     6361 aaaccaatac ctaccaagat agccaagtac catggagttc tattaggaag aacaccaaat
     6421 aataagaaat ctattaatcc tcctgaaaat gtgaatccaa ttttaactcc taataattgc
     6481 ataattgcgt atgataaacc tgaaaataaa caatgtattg caaataatac tggtgcaaca
     6541 aataaaaacg caaactctat tggctcggtt attcctgtta aaaaagaggt taatgctgct
     6601 gatgccatta tacctgctac aaattttttc ttttctggct ttgcttcatg caccatagca
     6661 agagctgctg ctggaagacc aaacatcata aatggaaatg cacctgtcat aaaagtacct
     6721 gctgtgaaat ttactccatc tttaagttga gcaaagaata tcttttggtc acccattaca
     6781 agctcaccag ctttatttac atattctcca aattgatacc aaaatggatt atggaaaaca
     6841 tgatgcaaac caaatggtat taatagtctt tccaaagaac cttccataaa tgctgctagt
     6901 gtttgatttg cttctataac attttttgag aaactaagta atccctcttg tattggaggc
     6961 caaataaaag acaatataat tcctattgct atagcaacta atgaagtaac gattggtaca
     7021 aatcttttac ctgcaaaaaa acctagatac tgaggtaatt caattttata aaacttttta
     7081 taaagataag cagccactat ccctataata ataccaccaa atacccctgt ttgaattgtt
     7141 ggtattccaa caatatttgc atacatagaa ttgtctacca tatcttctgt aacacctgcc
     7201 ataacaccca tagatgtatt gagaattaaa aaacctacta tggctgccaa tgctgcaaca
     7261 ccttctcctt cagttaaacc aacagctacc ccaactgcaa atataagtgg taagttatta
     7321 aatataattt gacctgaact ttccattata cttattataa gttgaaatgt aggattcgcc
     7381 aaagctggaa aatgagccaa caatgcgtct cctgctaaag cattactcac gccaagtaat
     7441 atacctgctg caggaagtaa tgcaactggt agcattagag attttcctac tttttgtaaa
     7501 acaccaaata cttttttcat ttacttttct ctccttatta ttttatattt ttttaaataa
     7561 aaaagactta agtttataaa aagttacatt agtatactta attctaaaat taaacttatg
     7621 ttaatataaa caaacctata ttaacctaat ttcagaatta aaaataaact attataattt
     7681 ttttataaac ataagtct
//
LOCUS       DAELTA010000162         7668 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.163, whole genome shotgun sequence.
ACCESSION   DAELTA010000162 DAELTA010000000
VERSION     DAELTA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.163"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            126..1400
                     /locus_tag="KPG13_003055"
     CDS             126..1400
                     /locus_tag="KPG13_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG0986751.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(1561..4602)
                     /locus_tag="KPG13_003056"
     CDS             complement(1561..4602)
                     /locus_tag="KPG13_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG0986752.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            4808..5107
                     /locus_tag="KPG13_003057"
     CDS             4808..5107
                     /locus_tag="KPG13_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG0986753.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(5346..7574)
                     /locus_tag="KPG13_003058"
     CDS             complement(5346..7574)
                     /locus_tag="KPG13_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG0986754.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         2658 a         1099 c         1082 g         2829 t
ORIGIN      
        1 aagaaatcta ataataaatt actagaggta tttaactatt tagaaagaat tggtattata
       61 taaagtaaga gaatatataa taagaaagaa tatttgattt tattggaaaa caaaagaaaa
      121 agaaggtgat tactatttct catataactg cgaagaatat gtataaaagt ttagaggaaa
      181 gaataaacaa gtttccacaa ggtgctccac catctgatac attatacaaa atactaaatg
      241 tactatatac agaacaagag gcgaagttag ttgctcagct tccaataaag cctttcagag
      301 taaaaacagc agctaaaatt tggagtgtaa gtgaaagtga agcatataga gtattggata
      361 aacttgctag taaggctctg atactagata tagaagataa taaagggaag aaatatataa
      421 tgcctcctcc aatggctggt ttttttgagt ttgccatgat gagaacgaga cacgatatag
      481 accaaaaact tttggcagag ctttactatc aatatatgaa tgtagaagaa gattttatca
      541 aagatttatt ttattcaaca gaaacgaagc ttggaagagt atacgttcaa gaggaagttt
      601 taactaatga taatgaggtt agtattttag attatgaaag ggctactcat ataattgatg
      661 aatcaactca tataggtata agtatgtgtt actgtagaca tagaatgcaa catgttggaa
      721 aagcttgtga tgctcctatg gatatatgta tgacgtttga caatgtagca aattctttaa
      781 taaataataa atttgcaaga cgtgtggata aaatagaatg taaagagcta cttcatcaag
      841 catatgaaca taacttagtt caatgtggag aaaatgttag gaaaggggtt acgtttatat
      901 gtaattgctg tggatgttgc tgtgaagcaa tggtagcagc taaaagattt ggaaatcttc
      961 atccagtaca gacaacaagt tttataccaa atataaacca tgaaaattgt gtaaagtgtg
     1021 gtaaatgtat aactgcatgt ccaatagatg cgataagcaa ggttaaggaa gatggtaaag
     1081 aatatataaa aattgatgag gatagatgtt taggttgtgg agtttgtgtt agaaattgcc
     1141 ataaaaatag cattatgtta ttaaagagag atgaaaaaat aataactcca gcaaattcag
     1201 tacatagagc agttcttatg gcaattgaaa aagggcagtt acaaaattta atttttgata
     1261 ataatgctct ggctagtcat agagctatgg gagctatact gtcagctata ttaaagcttg
     1321 aaccagctaa aaagatttta gccagtaaac aattaaagtc agtatattta gataaattgt
     1381 taagtatgaa tgataaatag aaactaactt aaaaaaggca aagttttaga cttatctata
     1441 tcttaataaa cttatcattt aatatttaaa tttaattttt acacatataa aacttataac
     1501 ttaaataaaa aaaggctata ttcaattaat attacttgaa tacagccttt tttagttatg
     1561 ttaagctatt tcttttattt gcttaaatga gttttcattt ccatttccac caatttgtgt
     1621 tacagattta aatctcatag ttttaaatag ttctttttga ttataatcta gtgagtttcc
     1681 tactagcatt acaggagatt tagtttttcc agccaaaact ccaacagata atccgtctat
     1741 taaatcatct tgtttattca ttccattttt agcaacatat aaattgtcta tcttagaatc
     1801 cttgtaaaat tcttttatta cttttgcatt agtttcattt ctagtgctac ctgatattct
     1861 ctgtggattt tgaagtttac tttctatatt tttagaaact gtatattctc caccgattac
     1921 ataagattta tcaatctttt tgtttttaaa caagttattt atttcagtta tattactatt
     1981 ttcattagta agtattatag gcatatcatt ttgagctgat acagctccaa tgcttactgc
     2041 atcggctaat cctttttcgc cattaactac tgaaatcttt gatatattgc taatattgtt
     2101 caattcttta gcaatgttca tagaagtttg atatctatca cttcctgaaa ttctagatac
     2161 agagatattg tttgatttta tagtatctaa agatttttca tttatagaag cttctccacc
     2221 aactacatat acatttttaa cttttaatct ctttaattca gccaaagttt tttcatttat
     2281 ttgagagctt ccagtcaata atataggagc gtttttcttg tatgcaaatg gtgttgcagc
     2341 caaagcatct gcaattgctg aatcgtttat taaaactgca ttttcagcat tttcccaacc
     2401 tgcttgactt atcttaatag aagtttcata tctatcagtt cctgtaagtg cacttgcatt
     2461 tacttcatct tgatatgtaa cctcaaatct agcatttgga cttcctaaga aaacaattac
     2521 gcctcctttt ggcaatctaa ctctattgtt ctttgtaact aaagaatagt ttacaggaac
     2581 acctttatca tcataaacag caaaagctga attttgagga agattaactt ctattttttt
     2641 atttgcgata tcgtcaccaa tcttatacca ttttgcataa ccattggatt ctagttcaca
     2701 agtaaaagat ttttctgctg gcaaattagt tatagaatct tcactaacat acgttagtgt
     2761 tccaaaagat aaatactctg taccattttc tttatgaagt ttaatgtcgg ataaatcacg
     2821 acctattaca ccaggtattt ctataaaagc atttgagtta ttctcatcca ttattttagt
     2881 gttaactata taacctggag tttcatcaga aagagcaaga gttgccttca cagaaccaaa
     2941 catatagctt tgagaagtat atttttcatc tactagatag taatctttac catttctttt
     3001 tttccatgct tcttttacac tatttgatat attgttgtca tctatctttt gagctacata
     3061 gtataaactt gctgtctgac ctaatccagg gacatcatca taagagctca tatttaaata
     3121 tgtgatattg tttttttctt ttacaaattt tagacagctg ttaccttttt cagatacaaa
     3181 tctatcttga cctatgtata cgtatttatc ttcaggtcca ttttctatat aaggagaaga
     3241 tactgttaaa gtaccattat cattaacatc tacttttatc atatttgaag aagcataaag
     3301 accactattt tcttttagag agctaggcat tttaacttgt tgtggtttac taaatgtttt
     3361 atcaggtttt atttctttaa ttttaccttt ttcttttaat gcagatagta agatttcttg
     3421 tccaattatt tcattaagtt gactacttcc accagaagaa agaactgcaa ctgccatatt
     3481 ttcatctgga agaactataa gattactgtg gaataataaa gagtctccac cttttgtaag
     3541 agcttttaaa ttatactgat taaatggata agtgttgaca caatcccacc ctaaaccata
     3601 tccaagtata gagtcttctc cttcaggcca tagaccattt aaatattctt tgttttccat
     3661 agcttttact gaagcaggag aaagaatacc atttgaattt ttcataaatg tttgtgcaaa
     3721 tgtacataga ttttctgcac ttgaatataa accacctgca cctatagcat taaggttatc
     3781 ttgcggaact gcatcttccc aataaggcac gtatgctttt gctagttttg aactatcaaa
     3841 actattttct gtagtttttg tattttgtaa gttaagtgga ttatttatgt atttatcaag
     3901 aaaattagta aatgacatac cagatactct ttctactaaa atttcagcta aagtaaatcc
     3961 atcattacaa taaacggaaa aagctcctgg tttagctttt aatctttgtt tttgtaattc
     4021 ttttaagaaa ttatcatgac cataagaatc attgtctgct aataaaatag tgtttttaaa
     4081 actacttccc atcaaccctg atgaatgatt taataacatt cttggagtga tttccttgta
     4141 tctatcatca gccattttaa attcaggtat atactttaca acaggagtat ctaaatttaa
     4201 ttttccatca tcaaccaact tcataacagc tgttgtagta aacattttac tgatagatgc
     4261 gatactatac atattatcct tatttaagtt tttgttatcc tgtttgctgt aaactccacc
     4321 attaccagaa atctcaattt tgccattatc aatcaaagca tattgagcac ttacagcacc
     4381 atactttgat agcaaagttt ggattttctc tttagcaaga gctttagttt ttgtgtaaga
     4441 ttccgcgttt ttactataat catgtaattc tatatcagaa ctttgatatt tttcggaatt
     4501 atcttttgta ttatcagcaa aagtaatatt tggagcagca gtaaataaca tactcaatac
     4561 taatgtacta gataataatt tagagagcct ttgcttaaac atattacatt acctctttcc
     4621 ctttttaata atttataatt agcataacat tcttaattat tttaatcaac agattattat
     4681 caataaaaaa agcttttata agtatagctt tttatataac agagaaaaat ataaaaataa
     4741 tatttgttgt tgtataaaga ataaagggta taggtaatat ataaataaaa tggaggaatt
     4801 ttagacaatg tataagaata taaataaaaa gcaacttaaa gaaatgatga aaaatgaaaa
     4861 ggatgtttta ttattagatg taagaactaa agatgaattt aaggaatata aaatagaaaa
     4921 tgctataaat atatctttac aagagttaat caataatata gatgaaattt acgattataa
     4981 agataagaaa gtagtagttt attgcagaag tggtcataga agtgttactg catgtaattt
     5041 gttagctgaa gaaggttttg agcatttata taatttaaat tcaggaataa ttgattatat
     5101 gagttaaata atgtaagtta tacattaaat actatgtata gctctgctta ttaagtagga
     5161 atatatctaa atatttataa gataaattta ggaaagaacg aattttttcg tttttttcta
     5221 tgacagtttt tgtagtaaat attagttttt aagttggaaa aaggtagtag ctttttagct
     5281 actacctttt aatatgaact tagaatatta ttaaaaaaaa tatatttaaa gacagaatta
     5341 attttttaat gaccagttaa attactatcg ccgaatttat cattaaatgg ttttataact
     5401 ctatttatag gtccttttag aagtacattt aaacaaatag acagaatcat aaatagtaat
     5461 agaactagta tatctttagt tagatttgga gcatatatac caccaactgc ttcccttgta
     5521 gcacctattg cataggtaaa tggcattata gggtttattc tttggaagaa tgtaggtgta
     5581 acctgtatag ggaatgtacc accagaagaa ccaatctgaa cgactaatag tactattgca
     5641 attgcttttc ctacatttcc aaatacagaa actaaagaat acactataaa gttaaatacc
     5701 atactagtaa acataagcag aactatcaat aaaattggat gttttgctgt tactcctaac
     5761 aataatatat ctccagttcc tattataaaa ccttggacaa gagctatact taaaaaagta
     5821 aggcctcttc caaagtaaat ttcataaggt ttatagtttc catgtacaga tgtagacaat
     5881 agtgctgaaa taagtaatat accaacccat gtagaaagaa cactataaaa tggtgtcatt
     5941 gcagaaccat aattttcaat tggatataat ttctctgttt tcacctcaac aggttctttt
     6001 aagaaattag actttgtcaa tatatcactt tcaagcaagc tgactaattt tttcatatct
     6061 tcaccattac taattttgct taaagtatca atcaaatcat ccaacattcc ttttgctaaa
     6121 ggaagtcttt ctcttatcaa tgatatactt tcctgtgcat ttcctgaaag ttttagagaa
     6181 gtagtaagta tttcttcaac ctttggaagt tttgcttctg ccttatctaa aacagtaatt
     6241 atatcatttg ctacttttat actgtttgca aatatactat ttataggttt tgatattttt
     6301 gagtcaaaat tatttaaaat atttaaatta attcttccaa taccatttga tagacttaaa
     6361 acattattca aagcacttat agatggttgt tgtccagata taactttgtt tttaatatca
     6421 tttaatgttg atatagatga atctatttta ttgctagagt cttcaagatt atctattaca
     6481 tcatccaatc tattatttga tgtaagttgg tttaatttag ttaaaaagtc caccaaagta
     6541 tcgtttaaag attgcaaatt agaaagtttt tctgataaat tatctatcaa cttaggtaca
     6601 tcttcactgc cactatttac tgcatcaatt aaatttaatg taagagaaga agcagaagat
     6661 gaaaggtcaa ccattaaatt taagtcagac ttaataagag gagaaagttc gtctaaatta
     6721 tcatttgtat cctctaagaa tttttttaag tcacttgata atagttttgt atcattcaga
     6781 gtctttttaa ttgtaggtaa atcagattta atatctttaa ctgtatcaga taattttgaa
     6841 gtagcatcag aagctaaatc aacagtctta tttatatggt caaatctctt ttgaaggtca
     6901 ataagggaat tttcaacttt tgtcaatttt ggaagactgt gttcaattcc tgcaccaact
     6961 ccgtttgata taccaagaac gatttcacta actgttttta ctatagtctg attaacctgt
     7021 agttgtattg tagaagcacc tttatctgta atctttggtg caactgcatt tattttttca
     7081 ttaacagtat agataatttc gcctttttta acatcatctg taattaaaga tgtcaaatct
     7141 tttgtaaaat tttttggaat cttaagtgat gcataataag tacctttttt aacaccttca
     7201 agagcagttt tttcatctac aaactgccaa cctaaactct tgttatcttt gagttgttca
     7261 attaattggt ctcctacatt aatcttttgt cctgctattt catttccttt atcttcattt
     7321 gttactgcta caagcatatt agcagtgctt ccataagggt cccaagatgc ctttatattg
     7381 aaccaagcat acagagatgg taaaaaagca agacctaaaa ctattacaag tacggcataa
     7441 ttgccacgta tatcttttag gtcactttta aaaatttcta atatattttt taaacccttt
     7501 gaaggtctta aattcttgat attattccag tttaaaattt cttttaaact attagttttt
     7561 ttcaaatgtc tcacctccta aaatattttg ataaagaaaa aagttagatt tataatcaat
     7621 tttaaattat attacttttt acacaaatag taaagttaaa attttgtc
//
LOCUS       DAELTA010000163         7664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.164, whole genome shotgun sequence.
ACCESSION   DAELTA010000163 DAELTA010000000
VERSION     DAELTA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.164"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..653)
                     /locus_tag="KPG13_003059"
     CDS             complement(<1..653)
                     /locus_tag="KPG13_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986755.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGN"
     gene            complement(1271..2485)
                     /locus_tag="KPG13_003060"
     CDS             complement(1271..2485)
                     /locus_tag="KPG13_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG0986756.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            2911..3627
                     /locus_tag="KPG13_003061"
     CDS             2911..3627
                     /locus_tag="KPG13_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG0986757.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            3762..4388
                     /locus_tag="KPG13_003062"
     CDS             3762..4388
                     /locus_tag="KPG13_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG0986758.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(4588..6132)
                     /locus_tag="KPG13_003063"
     CDS             complement(4588..6132)
                     /locus_tag="KPG13_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG0986759.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(6158..6799)
                     /locus_tag="KPG13_003064"
     CDS             complement(6158..6799)
                     /locus_tag="KPG13_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG0986760.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            7069..7260
                     /locus_tag="KPG13_003065"
                     /pseudo
     CDS             7069..7260
                     /locus_tag="KPG13_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         2791 a          992 c         1085 g         2796 t
ORIGIN      
        1 tgatttccct tcattctatg taaatgaata tcttttatta agttattaaa agtatttttt
       61 aataaatcta tatcagggta tatgaaatga aagtttactc ctgcattttg taacatagga
      121 acaatactac taaaacgagt agtagaaaaa tcgattttcc cttcattcca tagattaata
      181 tgtttttttg caatcttttt ttcaagtttt gctatgtcag ataaactcat tttagatgca
      241 ttttcatcaa tactatccat taagtctcca aatgtgccat ttgataaata atatgatata
      301 tcttttggat tatctgccca gtctgttaag tcaaagtatg ctctggaaaa atctaatgta
      361 ttatatttaa agataagctt tagaaacata tcatagtaac tttgtaagtc agtaccaaaa
      421 tcaactagag gttttttaat agttccagcc ttttttgtaa tagctttttt ggggatgggg
      481 ccactaactg caaatccatc aaacctatct tctatttcta aatataaatc tccaacatga
      541 gaaaaatctc tatagatata tatttcaatt tctgcattaa tatttaactt ttttatagaa
      601 ttatctacaa actcttttaa aaattcggtt gttatgactg ctattttcat catagattac
      661 ttccttttca attattataa ttattttata ttaaatatta cattaatcta ttgttctagt
      721 taaaatcgta tttcaattaa attcaattaa aataaataac tctttataaa aagcaattat
      781 ttaggtagag gaatatttgc taactttaat agtctttgtt aactttaatt ttatttaagt
      841 tattatagtc ttactaattt tataattgta gatttaacta ctttatgttc ttttattgtt
      901 tatgtagttg atttagaagt tattttaacc atttatttta attgaagttg tttgactttt
      961 aaatcctttg tgtaatcttt gtttttctag ccatgataaa agtatttcta tttgtatcac
     1021 tatatataat aatatcatga caaaatattt attgtctaac taattataat ctatccaaaa
     1081 tgaaaatggg tgtgagatat tattacaaca aattgtatta gtattaaaaa gattaaaaaa
     1141 ttaaaaaact aaacagttaa ataattaaac gattaagtga ctgtcatata acaatttata
     1201 attttattta acagccactt aggtatttaa gagcgataga attgagtgta tatcaaatta
     1261 taataatttc ctagtaaata attgaaaaat aaggtagtcc atttttatat ttgatatctg
     1321 gtttaccttg aataagtgga tatagataat ttagggcttc atcagttata taattacatt
     1381 cttcatttat ccactcttta ggaaaatatt ttgtcttgtt agctatttta gaaatatcag
     1441 tatgactaac ttcatactta taaggattat tgcatgttct attaatgata gccataactc
     1501 ctgtatatcc ttttttagat agttctatcc catatgcacc aacttcagct gcttgtttta
     1561 aatcagtatc tgaagctaaa tgcatagcac atctttgtat tacaccaagt tctaaggttt
     1621 ttactcttgt tgtaattgat gatgatacta taagattctt tagatagttg cttactccac
     1681 ctaattggga atgaccaaat ttatcatgag cagaagtttt ttgttcgcat ataaagttgc
     1741 cgtatttatc ccttatacct tctgatgcaa ctatataagc cttatttttt tctttaaata
     1801 tttttctaac atcttctata aacttatctt catagaaatc aatttctgga aggtatatta
     1861 aatcaacaag tggagtatca ttaagtctag ctagtgaaga agaagctgct aaccatccag
     1921 tatctctacc catagtttct actataaata taccattatt tttgtatata gaaaaatcta
     1981 agtatgtttc cattattagt gtagaaataa gctttgcagc agaaccaaat ccaggacagt
     2041 tgtcagttcc taataaatca ttatctattg ttttaggaat acctataaat tttattttac
     2101 aagtagtgtt tttgcagtat tgagaaaatt tcatcactgt gtccatagaa tcatttccgc
     2161 ctatataaaa taatgtatct attttatatt catctagtat atttataact ctatcataat
     2221 cataagaatt tattttataa tcagccagtt tgtatctaca agaaccaaga ccagacgaag
     2281 gtgtttgctt taaatttaat acattatctt tgtttaagga gtctgaatct attatatttt
     2341 tgtttaatat accttcaatc ccattgatac caaaatatac tttatcaaag tatttagttt
     2401 ctaaattttt ttcataaata ccaactgcag aagcattgat aacagctgta gggcctccag
     2461 attgggcaat aatacagttt ctcattttac atctcccttt cattaatatt gttatgaatg
     2521 gtacttttta tcgagtaaat ttatatttat agtctgatta tatattaaat gaactagcta
     2581 tatcaattga gttagtatag acaataaatg ccataagaat gtggtaaaaa actaactttt
     2641 tatatacaat taatgtctat aatttaccac aatataaagg tacttttaag gagtagtcaa
     2701 agtgttgtga aaaaaaatat atcagtgtaa tattaattta caaagcaaaa taagttcaca
     2761 gttaacaaaa gcagaataag ttcataaaag aaatatatac agcaataaaa gtaatgctaa
     2821 ataaaagtaa tactggatta aaaaatatta aaaaaatatt aaaaactaat atttaatact
     2881 aattaaagta aaatatggag gtatacaaat atgaaacaca taaaaatagc agcaggatta
     2941 gcacatgtaa attacggtca tatatcagcc gtagttaaag aagcaatgga tgcaggtgta
     3001 gactacgtac attcagatgc agcagatatg catgacttaa aaaatatgca acttatgggt
     3061 ggtcatcaaa taatagaagc aataagaccg gtgacagata aaccaataga atgccatata
     3121 tacacaagag attgtgatag attatttata gaaaaaatag cagcagcagg atgtaacttg
     3181 ttgataatac cagcagaaca ttttttaggt gctcctcttg catacataat aaattactgt
     3241 agagaatttg gtatgaaagt aggattaaca attggatgtt acacaccact atgttttgtt
     3301 gatgaagcta tatatgatat agatagattg caaatagtcg ttcatggtgt tgatgaaact
     3361 gatggaaaag ataactgggg atggagaaag agtgctgttg atttagtaaa aagagctaga
     3421 aaaatgatag acgaaaagaa tcctaagtgt gaattagcaa tagatggtgg actaagagca
     3481 gacaatatgg agccattaat agaatgtaat ccagacgttg taatattatc ttcagcaata
     3541 ttcaaggata aagatggtat aacagcaggt gttaagaatt gtagaaaagc tatagatgaa
     3601 gcagctacaa agtttggttt agaataagat aataaactat gataaaagag atgctttctt
     3661 aatgttttaa ggaagcatta attttatgaa aaactacagg aagctagact aatttaattg
     3721 attttttata ctgaaacaat ataatcatag gaggttatta aatgaaaaaa gaaatgataa
     3781 aagattattt actgataaca atatcagcat ttttaatggc attagcagtt aattttttct
     3841 ttgcagaaca tacattagct ccaggaggga taacaggatt atcagtcgta cttagcacat
     3901 tcttatcaat gcctgttgag aatatatcat taggaatatc tatgccactt ttagtaatgg
     3961 gattattatt tttagggaaa ggttttggaa taaaaacatt atatataact cttatgggcc
     4021 cattattctt aaaagtaata ccacaaactc acataactga caatctatta gtagcaggag
     4081 tgataggtgg gttattggta ggaactgcta tagggatagc aataataagg ggctgtagta
     4141 ctggtggaac tgatttagtt gcaatgctta ttaataaggt aattaagttt ctgaagttgc
     4201 cagttatatt atttatgcta gatggattta taatagtagc gtctggaata ataagtaaga
     4261 attttatgat atctatatat tcattgatat ctttattact cataataaaa acaataggat
     4321 ttgtaactac aaagtttggt aagaatactg aagaaattaa taatataaat aatgcttgtg
     4381 ctcaataaaa ataataaaat ttactttaaa tataaaagtt taaaaaatat aaaagcttaa
     4441 ataaaaagat taaatataaa aaatgaaggt gtctcaaaat gaatttttta gttcatgagg
     4501 cactttttct atgaaatcaa atatattttt atcatttgga caatgaaaaa taggtttatc
     4561 tctatgttga gacaagccta tttttgttta tttataagat aatattttat ttttttcaga
     4621 atatagtttt tttataattg tactaagtct agaaatatca ttcttactta aaagagcatt
     4681 tatatttatc agttcagcat tatgttcaaa gtctacagga gcagtggtta gtattaagtc
     4741 cacatcatca aagttcgtaa atgaaataga agatagaggt atagatttaa ctatattaat
     4801 ctgattaaat tcaaaactta acttttggac taataaatta gatacaccat cactacagtg
     4861 acatattaaa atagtattta ggggagattt gcttctatct attgctgata ttaaatataa
     4921 agcaagatat cctatttcgt catcattgac ttcataattc atcaactgtt tgataacagt
     4981 attagactct ttagcagcct tataagtgta aggaaatgtt ctctttatat catttaatat
     5041 gggatttgta atagtaaacc catagtttat tctgtgtaga actgatttaa ggtgattagc
     5101 aagagacttt attaatatta aatcatcctt taagtttaaa tttagtatac ttccccaatg
     5161 aacagctatt gcttcagata gattatactc tgtaatttct tcatttttaa gaggttcaag
     5221 agtagtagtt ttctttattg atttaagtaa tccttctata tgaagaagga tataacagac
     5281 ctcttcatca ataaattcta cgtcaaaagc ttcacttagg ttagcacata aaatttttga
     5341 tttttcaaag tattcatgtt ctaataattc tctaaaaaaa ccttcagata agttaatata
     5401 tcttttaatt gaaattcttt taatacatat agctatatgt attaagaagt ttatataata
     5461 ctcatcacta aggttacttt tttctacttg taaggtgtgt agagtgattt gagataattt
     5521 ctcaaaatca tagttaaata gctccttaaa tatttttgtt gatggggtat ctttgcattc
     5581 attagatttc gagaataata tttctttaag ttcagtataa cttttattaa ccgtagcaag
     5641 ttcaaaaatt gcttttcgaa tatctttctc ttttccctct aaatatactc cattattggt
     5701 ttttctaact agagtcactt taaagttttg caaaaaattt tgaataacta ccaagtcttt
     5761 atgcacagta gctctactaa catgtagttc attgcaaata tcacgaatcc taaacttaga
     5821 atttttaata aataatttac ttagaatata cctttttcta tcttcaggag agtatgcata
     5881 tatacaattt gatttatcat tgatatctct aataacttta agttttatat cttcattacc
     5941 tagaataata acccctgaac cagttttttt atctagggat aaattaaact ctaaaagcca
     6001 ttcatctaaa ataacaaggt cattacgaat agttttatta ctaactttta attcttcggc
     6061 aatagtatct acagtaatat agtctgagct gttgagtaat aattcagcaa tatctaataa
     6121 tcttttgttc aatgcaaacc ctcctaaatc ttagttacta tttattaacc ttggaccaat
     6181 ctctttcaaa agtattggca ccatctattg taagctggtt aaaagaagct tttttaaata
     6241 aatcatatgt tacagctgca taatcacatc cacaaagaga tgcttgagtt aagtggaata
     6301 catttttaat agaagctgca acaactttag tattatatcc atttattgat ataaggttac
     6361 atatttctgt aaggtttttt ataccatcat tcccattttc atcatttcta cctataaaag
     6421 ggaataggta atcagcacct gcatttatag ctaatgatgc ttgatttaca ttgaaaatca
     6481 aggtacatgc agtttttaca cctttactat ttaatatgtt tataagttct aagccaactt
     6541 cagagaaatt tatttttatt atagtgtttt tatttatatt taatatctta tcacatgaat
     6601 taattaattc gtataaatta tttccaatga cttcagcagt taatatttct ggatttaaat
     6661 tagcacatgt ctcaataaat tcatggaagt ttactttctc tttaagatac atagatggat
     6721 tagcggttat tcccttaaat ccatacttta gacacttttg aatttcatca agattagcac
     6781 tgtctatcaa atatttcata atttaattct cctttaagct atttgtcata aaaaagtgta
     6841 tatactctat ttttattatt aagtcaatta tacaataaga ttgattataa aattataaca
     6901 tgggtactta ataaaattta cataatgata tggtaataat aaaacattga ttgtctaata
     6961 attaccataa gctttagtaa ataagagtta acagtgaagg tattgcctta aaaatcctat
     7021 gatatgtata atttgaatat gaaggcaata atttaaggga ggaaaaatat gattaacttt
     7081 tatcaaccat caagaataaa ttttggacaa ggaactttaa gtgaattacc tagaataata
     7141 ggaaaatatg gaaagaaatg tttgttagtt actactccaa atgtagagcc attagacaag
     7201 ttatataaaa gagttacatt gaatttaata gaaaaaggta tagaagttgt acactttgat
     7261 taaaaaatag ctacgcacac tcgtgacttt agtcatgagt taatagctaa aatagtcagc
     7321 atatagggaa acctatatgt agagatagga tagaacctat ccaatactac ttgaattgct
     7381 ggaaacccct aaagctaacc aaactaaaac ataaggatga aataaaccta agtgtgaaag
     7441 ttacgaaagt agaaaaaatt ggttagatgg tataaggtta aatcctaagt actttaaaat
     7501 gggcaatcag caggtaagct ccgaatagga gaaacttcaa cgactattcc tcttgaggga
     7561 agtacactac aagttatcgg tagtggaagt gggtagacct taacggataa tgccgaagga
     7621 taagatatag tctgtgcttg catgaaagtg taagaagttc ataa
//
LOCUS       DAELTA010000164         7638 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.165, whole genome shotgun sequence.
ACCESSION   DAELTA010000164 DAELTA010000000
VERSION     DAELTA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.165"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(601..1152)
                     /locus_tag="KPG13_003066"
     CDS             complement(601..1152)
                     /locus_tag="KPG13_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG0986761.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(1303..1566)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2002..2400)
                     /locus_tag="KPG13_003067"
     CDS             complement(2002..2400)
                     /locus_tag="KPG13_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986762.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     gene            complement(2756..3241)
                     /locus_tag="KPG13_003068"
     CDS             complement(2756..3241)
                     /locus_tag="KPG13_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG0986763.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(3324..3417)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3771..4154)
                     /locus_tag="KPG13_003069"
                     /pseudo
     CDS             complement(3771..4154)
                     /locus_tag="KPG13_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      4380..4477
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4532..4708
                     /locus_tag="KPG13_003070"
     CDS             4532..4708
                     /locus_tag="KPG13_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986764.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            4934..5134
                     /locus_tag="KPG13_003071"
     CDS             4934..5134
                     /locus_tag="KPG13_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG0986765.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            5886..6068
                     /locus_tag="KPG13_003072"
     CDS             5886..6068
                     /locus_tag="KPG13_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBG0986766.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            6139..6984
                     /locus_tag="KPG13_003073"
     CDS             6139..6984
                     /locus_tag="KPG13_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0986767.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(7208..7372)
                     /locus_tag="KPG13_003074"
     CDS             complement(7208..7372)
                     /locus_tag="KPG13_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986768.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         2780 a          991 c          773 g         3094 t
ORIGIN      
        1 attatgttat tttaatagat ttatttttct tttttaagca tagagatgta tttttccata
       61 tattctattt ctattatagt ttcgcttatt taattcacca aaaatttcac ttaatattag
      121 aattttaaat cttttactaa aacagacttt tatagttcta ttaaaatttt ttactaaact
      181 ttatttttta ttatgaatta tctatttttt ttggctaaat ttaagcaagt ttttcacaaa
      241 aaatatatac attactatat ttttaaaact ttataaatat ataattgatt taattggaaa
      301 ttttagtgta ttcaaaaaat gagaaatcag aggagattaa tataaaaatt tatcttgaaa
      361 agtcaattaa atatatctta tatttaataa ctacaaatga gatacatata agaatattat
      421 ataggtatga taatttatct gttaaattaa gtgtaattgg tactatataa atattttatt
      481 aaaaataaat atataatacc aaatctttaa acaacataag aataaaatag ttataatcta
      541 ctttattctt atgttattta gttattataa tgctttataa agtaaaatat tatttcgtgt
      601 ttaagcttga taactaactc ttcttattga tagactaaca atcttgaata aaaatattcc
      661 tgcaactaga tttattaaaa cagctggtaa tactactgct aagaataaag ctgtaaatga
      721 agcccctgga agaccaacta tagcttgagc tgctaataaa aagatagttc cacttacaag
      781 agttcctatt ggcattatta ttgcaactac tacagtatct tgtgttttct tagctaattt
      841 ttttataaaa ggcatattat aactaatatt cattaataaa aatactatat ttgctgtaag
      901 agttttatct attacattag gaatttgacc tcccggaaaa cttgatgtaa gtgctgtaaa
      961 tattcctgtt actatacccg ctatcaaaca agttttataa tcatctctat ttaaaaccaa
     1021 tattacaaat aacattgcta gtggtacatc tggcttcatt ggtaatccta tagctggtgt
     1081 gatttgatgt attaataatc ccatcgctaa aagtattgca tttaaagtta atttttttgt
     1141 atttgttttc attttttgta cttcctccta tttttttaat tttcaattta tttttttgcc
     1201 aacctactat atcatcatct ttagcttttg aaattcataa catctttctc accaccttct
     1261 atcttttaaa ttttcagtaa aataaaaaac tccgtcctaa actaatagga cggagattat
     1321 ccgcgttgcc acctaaatta tataaaaatt aatataaaat tcaattttca tacatctcat
     1381 tatcaggtac ttacatacct tattcgctat aacgtgcgac tgacgataaa gtctactctc
     1441 aaaactatga tttcaattta ttcctctaag ggccattcat caaatttttt cttgctaaga
     1501 ttccaccatc ctcagctctc tatactgaac ctaaatttaa ctactcttcc ttgtcatagg
     1561 atttaattta ttgtattgaa ttttgttgtt aaaataatga tattcttttt tctaaaaaat
     1621 gtcaatacct ttttataaaa aatatatttt gtaatctatt ttttataaaa aaagtaaatt
     1681 atactataca tttcttttca tatacaaaat tctatgaata attactttaa tggtctaata
     1741 gtctgctctt tggctctaat tcttcttata ttaattttgt gaataactgt tgatgtaatt
     1801 ttatattctg attcatgagg aattaaattc ttcaaagtta attaagtaca tttatcaata
     1861 aatttactta attaaccatc atttaatact cattcctgta tttatatgcc tcctttgaaa
     1921 cagaatatta ctccatttta attacatcat attattaaat agataccaat gattaaaaaa
     1981 atgatattct tttaattatt tttagtaaaa ttttttcttc tttcttttaa taaatattag
     2041 cactactaaa actataacca aaaatatgac tattatggac aatattaatt ttgttaggat
     2101 attatgtctt cttgcgtttt gtcttttata tttatttata gtttgatatc catacttttt
     2161 agttcttata cttcgttttt tcaactttac tttactttta ttatatttcc tacttttatg
     2221 tctactttta gatttaatag ttttaaattt agtattatca tatgtataat tctcttttat
     2281 tgaagaaatg tatacactag actcaataat agcttctgct tctacttgta atggatttat
     2341 aatgctgatt ataagtagaa aagatatagc tatcccaatc aattttttct tcttattcat
     2401 attttccccc tcataaaaat ttttaactag ttcattataa tatatatata actaaaaatt
     2461 acaaattttt tcaagtttct tatatatctt attatcttta atattatttt tattatcata
     2521 acttttactt tttttaagat ttttcaaagt agtattaatt tatgatactt ggtgtataaa
     2581 gtataagtta aatctacttt caattaaatt taccatttat aattacttta taataaaaat
     2641 aaattagtta atttattctc tttagttctg ttataatgtt tctacaaaaa agaatcccat
     2701 taatgggatt cttttttgcg atttattaat atatttaaag cttgctagta tcgtattata
     2761 aacttaaatg ctcttctatg tattgattat atacatatcc agaatatcgt ttttcatttt
     2821 tattgatatt aactcttgcc cattttccaa cgctataaca tacttcaact ttaaaaccat
     2881 cagggattgt agttataact ttagattcta atgttctatc atttctaagt tctaaactta
     2941 atccatttgt tcttatattt cctatgtaat tcttttttct attttttatt gtatcattac
     3001 tattgttttt agcattactt aatctttcta aatatttata aagtaaccaa cctttgtcat
     3061 aattactctc aacttctgcc catattccat ctacacactt taacttaaca attttacctt
     3121 ctggtatact ttttaatttc aaactattta tgtctggttt ttctcttaaa ttcaatttaa
     3181 ttttagtctt aaaattgatg ctagatttgc gaaaattttt taacattaat tcagatatca
     3241 tcttaagccc tcctcttgcc aaatattaag ttattaaaat atataactct aaaaatactt
     3301 ttaacaaaaa gaattattat ctttttggca actggctgac atgaccttta ttacataaag
     3361 attttagtcc ctatagtttt gcgtcattag atttatctaa tattgcctat atctgaataa
     3421 taacataatt tgaacaatta aaaaataata ttacctcaaa ttttatatta tttaaatata
     3481 aattaaattt ttaattaata ataatcaaaa aatatcttta tatttattca attactgttc
     3541 tttatagtca atataaaaga cagtaattta tctatagtgt tacttatatt ttatatatat
     3601 ggctattttt caacatttta caatatataa catttatttt tttaactttt gtcagtaaaa
     3661 tgtcagcaaa acactttatc cacaattttt atataagttt tccacaactt atcaccaata
     3721 ttatccacat aaaaaaggta gcacttatat tattagctac cttttattta ttaatcttta
     3781 tatttccagc tttttatcca acttcctatt tctattattt tttcttcagt tatttcttct
     3841 ccatcatgta attttgaaat taattctgat aaaggattgt tatgtaaacc tctaaaaact
     3901 ctttttccct ctattttgag atattctctc tcttttatta atactgtata gtgtacatgc
     3961 ctcccaattc tctatgatat taaaaaacct tttttggtta atctagctaa aagtataatt
     4021 gtagttgtat gcttccaacc atatgttttt tctatttcat ctgcaacatc ttttgatatt
     4081 acagttttat aaccagtatg ccatatgcat ttcataattt ttaattctgt atctggtaat
     4141 tttttattta gcataatata ttccttcttt caatttatgt cacttcttca aatttatcta
     4201 gcttaaatta taataggagc tttttagaac aaaaactata tttaattttt accaggtact
     4261 ctacgtgttt agtctgtttt taagtgtaaa ataatgttca tatctataaa tttatttaac
     4321 tttcaaacaa tcctgttgaa taataactaa ttctattgta ttattttagt tgtagaataa
     4381 aactaaatcg gcaaaactag agaaatttag tgacgcaaag ctatagggac taagacttat
     4441 ataaatatct tatgagttat gtcagccagt tgccaaagag atagtgttct ttttgttttt
     4501 atgaaagttt tttaggggga taataattta tatgtttgga aataaaatgg ataaatgtac
     4561 acatgtattg actgcttata ttagcagttc gtatgattat tgtaattttt tagatacaca
     4621 gttagatgat tttatattag agtacggaga aaatgtagta gagtcttgtt tacaccaagt
     4681 aatggtattg gtaagtaagt ataattagat agacattgat ttctagaaaa ccctttttag
     4741 ttttatctac atttatatta atatatagca atgtaaaacg tttaaatata tttttaatat
     4801 taagaataat taaaatgtta actaaaaaat ataattaagt agacatttct attaatctat
     4861 gatacaatag tatttgtggt aatacacaat taaatcaagt atatcttgat ataggttaat
     4921 aggagtattt ttaatgaata atggaatagt gaaatggttt aataatgaaa aaggattcgg
     4981 ttttatatca atggaaggta gagatgatgt gtttgctcat ttctcagcta tacaaacttc
     5041 aggatttaaa tcattagaag aaggtcaaca agtaagcttt gatatagtta aaggtgctag
     5101 aggtcctcaa gcagaaaata taactatatt ataatatata gatttttcgg gaaagactct
     5161 ttttagagtc tttttttatt gttaatatat gtaagataat cattggtgaa gtaaataagg
     5221 tatattgagt aatgttcttg atagtttata tgctaatgaa tttcaaaaaa ataagcactc
     5281 ctataaaaag agtgctctta atgtaaatat aaaccttcat tggtccagcc taataccccg
     5341 tattctccaa gttctaacta ttttttatat acattatcac tttcatccat atctaacaca
     5401 attatttctt ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt gacaattatt
     5461 tgtgttttta ggagaattgc ttttaatgta ataaaaattt tacataccac atagttgata
     5521 taaaactata ataattacat aagattgtaa taataagact tatctttaca taccacatag
     5581 ttgatataaa acaatgaaat taacatacaa gagactaaga caattaaaat ctttacatac
     5641 cacatagttg atataaaacc ccaaaataaa cttagcatct ccaatgttta cacatataca
     5701 atgctcttaa aattgcagtg aacgcgcaat agtgcaattg ataatactta ttatatattc
     5761 tcaatgcctt atatttcaac tgctaaacct caatttgtca caaatatcgc tcactgcaaa
     5821 atttctatat ttttattata tcataacata taattaatat tttcaattaa tttttacatt
     5881 taattatgtt taaattctat aataatgcaa ctttaatgtt tctttctgga actaatataa
     5941 aaactatttc agaaagatta ggtcatgcag atataaaaat aactatgaat agctactctc
     6001 agtttttaga agagatggac agagaagcat ttgaaaattt gagcaaaata ctatttatta
     6061 tattttaaag aagatgactt attcaatatt tgatgctaga atataattaa taaaaattta
     6121 attataggtg attgtattat gaatattatt aaaagcataa ctttactctt aacattatta
     6181 attgtgatat taatgacagg ctgttcccca agagaaagtg ctagtgaagt tgcaaaaaaa
     6241 tatattgata agatgaactc tggtgactat gaaacagcat atagtttatt aagtaaagat
     6301 agtaaaaaaa aaattagtat agaaaatctt gaagaatttc aaaatatttt agttagtaca
     6361 aaaagaataa ttagttataa aataggtaaa gaaaaaatca taaaaaaata taaacatgat
     6421 ggaaaagaat ataaaaatgt tgttaaatta gaagaaactt ttaatataaa aaaccttctt
     6481 tataaaaaag atgattctct aaaagtaaat agatattttg tagttgaaaa taacaaatac
     6541 aagctattat tgtatgaaag tttcaaaaaa gaatttggta ttaatagtat ggaccttgct
     6601 caacttaagc ttaatgattt tggactaaaa tactttaaag aagttgacct tatactaaaa
     6661 aaagctattg ctattaatcc tactgattca aagctttatt atgcccaagc atgtaattat
     6721 atgaattttg atggatttcc tggttattca gatacaacat ataatgcttt agattcaatt
     6781 aataaatctt taaagtattt agataaaaat gattctgact acaaaaaaaa tgcaagtaat
     6841 atatataact taaaaggagt tattcttatg tttcacaaaa gatttgatga agcacaacaa
     6901 tatttaaata aagccttata ctttaacaaa gataatgaag acccaaaata taatctaaat
     6961 acaataaaaa aacatctaaa ctagatattt aatttttgaa tttataaaat atttttttat
     7021 gtataattca gcactgaatc ataatttcta ctaaactaat tacaagttta aattataaag
     7081 acttatttct atataaatat tactctataa caataagttt cttatttaaa ttttaaaata
     7141 ttatctaaaa atcataaaaa agaaggtata aagagctacc ctcaagctca atatacctta
     7201 taaatatcta aacttcattt tttactaatt tttctatatc tttgataata tgttctttag
     7261 atttttttcc agctatggca tcaaaaattg caacagcctt tttctctcca aacttttctg
     7321 ctacatcatt aaagtctaaa aatctaacac tttcttttgg tttaccaaac atataacacc
     7381 tctcatacat ttttatatat ttattataac ttctatagac ttatttttca ccaataaacg
     7441 ttagacaaat tagtcccttt atttttttaa actagcttta ccttcaaaat tcttaacaaa
     7501 aacagtttat tctactctat aaataaaaat agacaattta atttgtagga aactgataat
     7561 agaatacagt aaataaaact ttgtttaatc tatctgaaaa agttatctat taaaactgct
     7621 atgaatttat aaagaata
//
LOCUS       DAELTA010000165         7551 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.166, whole genome shotgun sequence.
ACCESSION   DAELTA010000165 DAELTA010000000
VERSION     DAELTA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.166"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            122..793
                     /locus_tag="KPG13_003075"
     CDS             122..793
                     /locus_tag="KPG13_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0986769.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            795..1703
                     /locus_tag="KPG13_003076"
     CDS             795..1703
                     /locus_tag="KPG13_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0986770.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1780..2466
                     /locus_tag="KPG13_003077"
     CDS             1780..2466
                     /locus_tag="KPG13_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986771.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2463..5018
                     /locus_tag="KPG13_003078"
     CDS             2463..5018
                     /locus_tag="KPG13_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0986772.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5212..6411)
                     /locus_tag="KPG13_003079"
     CDS             complement(5212..6411)
                     /locus_tag="KPG13_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG0986773.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6530..7417
                     /locus_tag="KPG13_003080"
     CDS             6530..7417
                     /locus_tag="KPG13_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG0986774.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
BASE COUNT         2974 a          887 c         1138 g         2552 t
ORIGIN      
        1 ttttattttg aaaacaaatt taaatacaat ctttttagtg aaataaataa aattaaaata
       61 tttttagtat gataatgcaa gaaaataatt atttatctaa aaaatctaaa ggagagtaaa
      121 attgaactta ttaattattg aagatgatat aaacttaaat gaaggtcttt tttatgcatt
      181 tgagaatgat ggatttaatg tatttaaggc ttatacaaaa caagaaggat taaatatatt
      241 taatagtaaa aatatagatt ttataatctt agactgcaat cttccagatg gagatggatt
      301 tgatgtatgt caaataataa gggaaaagtc agatatacca attataatgc ttactgcaag
      361 agactcagaa attgatgaag taaaaggatt agaaataggg cttgatgatt acattacaaa
      421 accattttct ttatctgtat taaaggcaag agtcaaagta gcaataagaa aaaaatcgaa
      481 taataaagta atatattcca atggaataaa attggaccaa aagttactaa aagtatataa
      541 aaataaagaa tgtttggaac ttagctctgt agaatataag cttctaagtt atctaattga
      601 aaataaaggt caaatattat taaaggaaca aattttacac catatttggg acagcgaaga
      661 aaattatgtt gatgataata ttgtatctgt aaatataaga agacttagag taaaagtaga
      721 ggatgaccca tctaatccaa aatatataaa aacagcttat ggaatgggtt atttatggaa
      781 tgaggtagaa taatatgttt atattagcct ttataatatg tacagttgtt atatttatag
      841 tatctactaa aataaatcga aaaagatatg atgaattaaa tttgatgtta aatcaaatat
      901 tggaaggtaa agaagttact tatccagata caaaggatac aagagcgtca aaaatttctc
      961 accaagtaaa aaaaattaag gatatgatag aaatagaggt agaacagtca aagtcagaaa
     1021 aagaagcgat taaaggttta atatcaaata tgtcacatca attaaaaact ccactttcaa
     1081 atataaccat ttattgtgaa cttctggaga atataaatat ttccacatta cagaagaagg
     1141 agtttttaca aaagatgaaa aatgaaactt ttaagattga ctggctgtta caatccttat
     1201 ttaaaatgac taaattagag gatggagtta tagaatttga agtagaagaa ctactaatta
     1261 aagatactct tattcaaagt atcagtacta tctttaataa agcagaagct aaaaatataa
     1321 ggataaatct cgaaccattt tcagacataa aattagttca taataaaaag tggaccatag
     1381 aagcaattgt aaatgtttta gaaaatgcaa taaaatactc accatcagat agcacgatta
     1441 ctatttctgt aataaaaatg gaactatata cgaagattac tattaaagat gagggaatag
     1501 gtattgatag tagagaacta aatgatattt ttaaaagatt ttacagaagt aaaaatgttg
     1561 aaaaccaaaa tggcacaggg attggtctat atcttacaag attaatttta gaaaaagaaa
     1621 atggaaacat tatagtagaa tctaaactag gaagtggatg ttgttttagt atcttcttac
     1681 agaattgtaa gagtttaaac taattttgta ggatgagtgt aattatcgtc acttacaata
     1741 ttttatagaa catatatctg tattgaaagg agaaattaaa tgagtatatt agtgacgaat
     1801 aatttagtaa aacactatgg aaaaggtgag actaaagtaa aagccttaaa tggagtaagt
     1861 atagaaattg aacgagatac atttactgct atagttggaa cttctggttc tggaaaaagc
     1921 acattgttaa atattatagg tggattagat aatccaacct caggtgatgt aataattaaa
     1981 ggaaagaaca tatctaaaat aggaaaaaaa gatttgacag tgtttagaag aagaaatata
     2041 ggatttatat ttcaaaacta cagtttaatg cccgtattaa acacttatga taatatagct
     2101 cttcctgtta cattagataa aggaaaccat atagaccatg aatatattga aatgctgatg
     2161 aagacattgg gaatttggga taagcgttta aaatttccaa gcgaactatc tggtggtcaa
     2221 caacagaggg tagcaattgc tagagctttg gcaaataaac ctgcgcttat attagcagat
     2281 gaaccaacag gaaatctcga tagtaaaact acaatggaag tggtatgttt attaaaagaa
     2341 agtagtgcaa aattccatca aacgatactt atggtaacac acaatgaaaa tatagcacaa
     2401 gtatgcgact caattatcca tattgaagat ggagttgtgg tgaatactgg tggtgaagta
     2461 ctatgatgtc tctagtaaaa ttttcaattc aatatataaa gcattataaa aaacaatcta
     2521 tatctatagt tttaagtata gtactatcag tagcattatt gacgggaatt ggttctttag
     2581 ttcatagtgc agacaaaagt agaattgaaa agataaggga agatagtgga gattatcatt
     2641 atttctataa agtagataag gaacaactta ataaaattaa aaaaaataaa aaatcaaagg
     2701 aatacactgt agatagactt ggcgttacaa aacctaaaaa tcagatagat gaacctttta
     2761 ttatagaatt tttaaatgct gattcttcat atttagacat gacaggtaga aaattattaa
     2821 aaggaactct tcctagtaaa gatggagaga tagtacttga cacatattct ctaaacaatt
     2881 taaatgtaaa caaagaaatt ggaacagaaa tcaatttaga tggtaaaatc tataagttat
     2941 gtggtatttt atcaagtctt tcaaacccag aaactgctct ggagggattt gtaaacgatt
     3001 attttcagcc taaagaaaag gattcttata tagtttatgt aaaatatgat gaaaataaaa
     3061 atattaaaaa gcaaaattat aaatttatga aaaaattcaa catatcaaaa gaaaaaactt
     3121 atataaactg gcctctttca gttgaatttg gagtaaaacc accaaaaaat atagaaaaat
     3181 ataatatatt tgagttctta aaaaatttag aactaaatac aaatgcaatt atattacttg
     3241 ttggagtgtt tggtgcagca gcagtatata gtattttcca tgtttcaata cttcagagaa
     3301 tatcacaata tggggtttta gaagtattag gcgctaataa taaacaactt ttatttctac
     3361 tatttttaga gttattttta ctttttattg ttggatttcc tataggttgc ttcttaggta
     3421 ttgaagtggc gtctacaata tatgaacaat ttccacatat atttttgagt agtgatattg
     3481 ttccaggagc attttttata agtgaaaaat ctatttattt aggcttttta tttatcttat
     3541 tgttgttaat tttaattgct gtacgtgttg tacatcagtt gaataaatac tctagtatag
     3601 aatctatgaa aaactttaag attactaata agaaaaagag aatattagaa agtgtaaaac
     3661 actctaacat gacaaaagta ttatctcata aatacatgac tcaaaaaaag ggaatgttta
     3721 ttggaatttt gttttcatta tcacttggtg gaattatatt tttatgttct agttactcta
     3781 tacagcttac taaaaataat aatgaactta caatgaaagc tgatgatggt ttaaattctg
     3841 actaccaaat aactatgcaa acaacagatt ttgatgtagg tataccaaaa gataaaattt
     3901 caagattaaa caatgttgag ggaatatcaa aagtctatcc agtaagatat ttatttggag
     3961 ctatgcaaat taaagaagaa caactatttt ggaaaaattt ctttaaacct cttgaaaaag
     4021 attacaggat aaagactttt tttgatggta tttgtacaaa gcaaaatgaa ggtggctatc
     4081 ttttaaaaac aaatatttat ggttataaca atggtatgtt agaaggatta aattcttata
     4141 ttatagatgg ggagattaat acccatgata tggttaaaaa taataaggtt atagttagac
     4201 tacctatgga tggaactggg atgtatgatg cagttgatat aaatccagga gatacaataa
     4261 aagttaaagt gccaaagaca atgaaaccaa cagatgaaat tgttaaattt aaagaagaaa
     4321 ataagaatga ttatacaaca aaagaatttg tagttgctgc tactgtaaaa cgtgtgatgg
     4381 caaataacat atatttcatt ggtgattatg gtatggatat agttatgacg aatgggcaaa
     4441 tgaaaaataa ctttgatatc gaaaattata attcagtaag tataaaaaag acagaagata
     4501 gtaacagtga ggaattgtct gagaacataa aaagtgttgt aagtgatgta aaaagatgta
     4561 tcgtaacaga ttataccatt gctattgaaa aaaataatac ctacttaaat caaaaactct
     4621 tatttatata tggtattgta tttgtacttc ttgcaataag tttgttccat ataatcaata
     4681 cagtaagcta cttaattttt tcaagaagac atgagtttgg aattttaaga gctatgggta
     4741 ttacggataa taagttttta cttatgatga taagagaagg ttttttatat ggattctatg
     4801 caagtattat aatggtaatt ggaagtgtta ttggacaatt tatgatttat tttatggtaa
     4861 aacgtgtata tttatatata aatccaattc ttaaaataaa tacacctcta tacataggta
     4921 tgattatttt aaatatcact attagtatag ttgctgtaat tattccagta agacaaattt
     4981 tgaagagtga tattatttca gaaataaata agaattagag ttatataaat ttttaagatt
     5041 atataacttt ctgacaatct tgtttaaatt atttaaatat atcttatctg tgaacattaa
     5101 ataaacctct taatagatat aactcttgaa aagtcatatc tattaagagg ttttaataaa
     5161 ttttcatgtt aatatatata atccaaaaaa attaaatctt attaaaacat actattttgt
     5221 atagttatcg aaatatcctt gtatgtaaat aataggagta cccttatctc cacttccaga
     5281 tgttaaatct gctaaagaac ctataaggtc tgttaatctt cttggagtag taccttgaga
     5341 aaccatgttt cctgttaaat catcagattt attatctttt tcaactatgt attttgaaat
     5401 tgcttctttt aactcatcac cagataagtc agcaaaatca ttatctgcta agtattttaa
     5461 ttttacttcg tttggagttc cctctagccc ttttgtataa gcaggagata ctactgggtc
     5521 agctaattcc catattttac ctacagggtc tttaaaagct ccatctccat aaaccattac
     5581 ttctacatct ttccctgtta tttcttttat attaccttgg attttgttta caacttcatc
     5641 acaatttata gggaataatt ttactgtttc ttctgtagct ttgtttgaac ctaaaagtcc
     5701 atactggtca ttgtaaccac taccatctac acttgctgtc attatatcat caagtgaata
     5761 tactttttta gctccatttt gtcttaaaat tctctttgtt ctttgtcttg tatgtatatc
     5821 acaagttaaa atacttgttg tatagttaag aatagttttt ggattgtttg caaatactat
     5881 ttctacctct gcaccacaat ccttgattaa attcttataa taatcaacat aatctacacc
     5941 agtaaatgta tgcttgttat atccaaatag ttctctgtat ttttcttctg ttaaaacatc
     6001 tgaccaagga tttattccct tatcatcaag ttcatcaata cttattaagt gatttccaac
     6061 ttcatcagaa gggtagctta acattaatac gacttttcta catccttttg caattccttt
     6121 taaacatata gcaaaacgat ttctacttaa aattgggaat ataactccaa cagtatcatc
     6181 tccaaattta tcttttacat cttttgcaat attgtcaacg tgagcataat ttccctgtgc
     6241 tctagctaca acagcttctg taacggcaat tacatctttg tccctaactt caaagttttc
     6301 actttttgac gcatttaaaa cagaatcaac aactatttta actaagtcat ctccttgtct
     6361 tataataggt gctctaaccc ctcttgaaac agttccaact aatctatcca ttatctaaaa
     6421 tcctcctaaa gaattaatat tgtcatatta gtaccatgta tattataatt caaatataat
     6481 tataagtaaa ataaataata ctaatatttg acataaggag taattatata tgaatataaa
     6541 acttgaactt tataaggttt ttaatgcagt tgtaaataat aagagttttt ctcttgctgc
     6601 taaggaattg tttatgtcac agccagctgt aagccagtca ataaagcaat tagaagaaca
     6661 attagatact ttattatttt atagaaacaa taaaggagta aaattaactc cagaggggaa
     6721 aattctaagt gaacatgtca ctactgcatt aaagttaata tcatcaggag aagatagaat
     6781 aaataaattt aaaaaattag aatacggttc tctgaaaata ggagtaggag atacagctgc
     6841 acgttttttc ttgttaaaat atttagaaat ttttcataag aagtatccac atatacatgt
     6901 atctacaata aatagaacga gtagagaact tatatcttta ctcaaagatg ggaatataga
     6961 tatagcaatt ataaacatgc caatagaaga tgatacacta aatattgttg agtgtattga
     7021 aatacatgat atatttgtat gtgcaaatga ttacatagaa tacaaaggta ggaaaatatc
     7081 attggaagaa ttaaacacat tgcctttaat aatgctagaa aataaagcaa attcaagact
     7141 ttatgtaaat gaatattttt tatcaaaagg tataaaacta aacccagata tagaattggg
     7201 ttctcatgaa ttacttttag aatttgcgta tataaatctt ggagtgtctt gtgttataga
     7261 agaatttagc atagattatt tggaaaatga gaaattgttt aaattagata taaaagagcc
     7321 aatacctaag agaaatatag gttattgcca tttaaaagat atatctttat ccttagcaac
     7381 caaagaattt ttaagcatga tatctaataa tatataattt agaatacata tttcacaagt
     7441 aatttaatat aaaaatagag ataaattcgt aaatttgaaa gattcatata gtaattagtt
     7501 tttattaaaa ataaaaataa ggttaatttg agaacattta tataattaat a
//
LOCUS       DAELTA010000166         7504 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.167, whole genome shotgun sequence.
ACCESSION   DAELTA010000166 DAELTA010000000
VERSION     DAELTA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.167"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..633
                     /locus_tag="KPG13_003081"
     CDS             124..633
                     /locus_tag="KPG13_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG0986775.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            645..1526
                     /locus_tag="KPG13_003082"
     CDS             645..1526
                     /locus_tag="KPG13_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG0986776.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1654..1859
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1985..3256
                     /gene="serS"
                     /locus_tag="KPG13_003083"
     CDS             1985..3256
                     /gene="serS"
                     /locus_tag="KPG13_003083"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG0986777.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3577..4032
                     /locus_tag="KPG13_003084"
     CDS             3577..4032
                     /locus_tag="KPG13_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG0986778.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4033..4124
                     /locus_tag="KPG13_003085"
     tRNA            4033..4124
                     /locus_tag="KPG13_003085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4067..4069,aa:Ser,seq:gga)
     gene            4162..4426
                     /gene="ffs"
                     /locus_tag="KPG13_003086"
     ncRNA           4162..4426
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KPG13_003086"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4503..6140
                     /gene="dnaX"
                     /locus_tag="KPG13_003087"
     CDS             4503..6140
                     /gene="dnaX"
                     /locus_tag="KPG13_003087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG0986779.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6199..6549
                     /locus_tag="KPG13_003088"
     CDS             6199..6549
                     /locus_tag="KPG13_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG0986780.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6562..7161
                     /gene="recR"
                     /locus_tag="KPG13_003089"
     CDS             6562..7161
                     /gene="recR"
                     /locus_tag="KPG13_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG0986781.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7302..>7504
                     /locus_tag="KPG13_003090"
     CDS             7302..>7504
                     /locus_tag="KPG13_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986782.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEE"
BASE COUNT         3055 a          752 c         1388 g         2309 t
ORIGIN      
        1 tcttttttat ttattataaa agttaagatt aaatagaata taaagtcaat attgatttat
       61 aataagataa aatataaaat tatatagtat aggtaaagtt tttatttgga aagaaggtgt
      121 aatatgaatt ctaatgaaag aagaagtaaa ttaatagata ttttaaaaga atctaagcat
      181 cctgtaaaag gtggaacact agctgaacta ttaaatgtaa gtaggcaagt aatagttcaa
      241 gatatagccc ttataagagc tagaggattt gaaataatag ctacacctca aggatatata
      301 atatataatc aaccgtattt tacaaaacaa ataaagtgta aaaatcataa ggattctaaa
      361 gaaatttatg atgaattaaa gataattgtg gatttaggtg gtattataaa agatgtcatc
      421 gtaaatcatc caacatatgg tcagataact gcagaactta atatttattc taaaatggat
      481 atagacaact ttatgaagaa agttgagaca aatgaattta aacaattatc ggttctgaca
      541 gaatgtagcc atattcatac aattgaggca ataaaagaag aaactataga agctatagta
      601 aaagagttaa aggacaacgg aatattatca taaggaggag ttgaatgaat tttttatcat
      661 tggctaagac tgcctcaaca aatagcaata taactaagag aactttagaa aatatgctcg
      721 ataattttag tgataatata ccaaatatta tatatgcaat aattgtattt gtgataggta
      781 tttatatatc taaaatagta agaaggatgg tttcaaagtt tctaatcaaa tatggaatga
      841 gtaaaggtgt aaataatttt attgtatatg gaatatatat atctatgtta tctattatat
      901 cattgataag cttaggtatt atagggatac aaactacatc agttgtagct gtgttaggag
      961 ctgctggatt tagtataggt ttagctttta aagaaatatt atcaaattta ggctctggta
     1021 tgattatttt attttttaag ccatttaata taggtgatta tattcaaggt tcaggtgtag
     1081 aaggtacagt ttctgacata caaatattta gcactgttct taagacgcca gataataaaa
     1141 ctataattat acctaatttt caactgacaa gtaataatat aattaattac acacatcaaa
     1201 acaaaagaag aatagatttt tcatataata tttcatatga tagtgatata gatgtagtta
     1261 agtctatatt aaatgaaatt tttacaaacg aaaaaagaat attaaatgat ccaaagccta
     1321 taataggttt aaactctata ggaaataaca ccatgcaaat agttgctaga ccttgggtaa
     1381 aaactgatga ttactgggat gtatattttg atgtaatgga aaaggtaaaa aataaatttg
     1441 atgagaatga tataaaagtt ccatttgttc cttcttcatt attatttaat aatacaggta
     1501 atctaaatac aatggataaa agataaggtt ttaattaaat agaaaaaatg aatttaatat
     1561 aaaaaattaa agaaaattct aaaaaaattt gaataatagt attgaaagat gttaatatat
     1621 ataatataat gattatcaaa taaaacaatt agttaaaaag ctgtgaagga aaatagtaaa
     1681 aaacagattg tattcagaga atgagtggat ggtgaaaact cattacaatg gtttttgaat
     1741 ctactctaga gcttctagtt aaagctagac gtttacctac gttataggtc tgagaggatt
     1801 atacatttta gtatagtcaa ttagggtggc aacgcggata aaagattttc gtccctttta
     1861 tagggtaaac ggaggtcttt ttttattttt aaaatttgtt taaactaaga gtttacaagt
     1921 ataatgtttg taaattaagt tggaattata acttatatta tcaaaattat taggaggaat
     1981 taatatgtta gatattaaaa gaataagaga aaatctagat gatatcaaaa aagctatgga
     2041 aagaagaggt gaaagagaat ttgaccttga tgctgtagtg gaattagata ataaaagaag
     2101 agaaatactt caagaagttg aagttatgaa aaatgagtta aatgtaaatt ctaaaaaaat
     2161 acctcaatta ataaaagaag gaaaagatgt aacagaagaa aaggcaagat tgaaagagtt
     2221 atcagataag ataaaaggta ttgatgagaa agtaaaagaa gtagaagcaa aaatggaata
     2281 tacattaatg agaataccaa atgttcctca tccagaggta ccacaaggtg aaacagatga
     2341 agacaatgtt caaataagaa cttggggaga acctactaaa tttgattttg agcataaagc
     2401 gcattgggat ataggtacag gtctaggtat tttagatttt gaaagagcag ggaaaataac
     2461 tgggtcaagg tttactttat ataggggatt aggagctaga ttagaaagat ctttaatgaa
     2521 tttcttttta aatactcata cagctaagca tggctatacg gaagtcttac ctccatttat
     2581 ggccaataga aatagtttta tagggactgg tcaacttcct aaatttgaag aagatatgtt
     2641 taaaatagaa ggattagagt atttcttaat accaacagct gaagttcctg ttactaatat
     2701 acatgctaat gaaatattag atgttgcaga gcttccaatt aaatactgtg catatactcc
     2761 ttgttttaga tctgaagcag gttcagcagg aagagataca agaggtttag ttagacaaca
     2821 tcaatttaat aaagttgaat tagttaaatt tgttaaacca gaagattctt ataatgaatt
     2881 agaatcatta actcatgatg ctgaaactat gttacaaatg ttaggtcttc catatagagt
     2941 tgtaaaaata tgtacaggag atttgggatt tacagctgca tttaaatatg atttagaagt
     3001 ttggatgcca agttacaata gatatgtaga aatatcttct tgttcaaatt ttgaagattt
     3061 ccaagcaaga agagcaggaa taagatttaa aagagataaa aaatcaaaag cagaatatgt
     3121 tcatacatta aatggttctg gattagcaat aggaagatgt ttggctgcta tattagaaaa
     3181 ttatcagcag gcagatggtt ctgtagtagt tccagaagaa ttaagacctt atatgggtgt
     3241 tgatgttata aaataaattt atattaattg tatatataga gtatgctttg atgaagagaa
     3301 tatattatga gtaaacaata aataaaaaaa ctatgaaatt attataaata ttaatttcat
     3361 agttttttta tgttaattta cacagcagaa ttttcatcat aaaacactaa atatttttat
     3421 aaaacactct tgtatgaata tctaaaaatt attttagaac taattttaaa aaaatatagc
     3481 tatatatagt aataattaaa taaacaaaat aaaattaagt tgaattataa aatgaattat
     3541 gataagataa gtttgttata agttttggga ggaattatgg aaagttcatt ttatatgaaa
     3601 gaagcattaa aagaagcata taaagcatat aataaaaaag aaacaccaat aggtgctatt
     3661 atagttaaag ataatcaaat aatagctaga gctcataacc ttacagaaac gttaaaagac
     3721 tctacggcac atgcagaaat tttagcgata aaacaagcat cagaaaaatt aggtggttgg
     3781 aggttgactg attgtgattt atatgttaca atggaacctt gcataatgtg tagtggcgca
     3841 atagttaatt ctagaataaa aaagcttata atagggacaa gacatgtaaa aaattcatat
     3901 atagaaaaac agcatgagtt taaattagat tattttaata ataataatgt aaaagtagct
     3961 tttgatgttt tgcaagaaga atgctcgatc atattgcaag aattttttaa ggcattgaga
     4021 aaaagagatt aaggagagat ggtcgagtgg tcgaaggcgc tcgcctggaa agcgagtata
     4081 tgggtaaaac tgtatcgtgg gttcaaatcc cactctctcc gccagaaaca atgaaagtaa
     4141 ttaaatatgg ttgtaatttt gctgtactag atggggaggt agcggtgccc tgtaacctgc
     4201 aatccgctat agcaggattg aattccatct aaaggcttat tttctgtagg gccgccctaa
     4261 ataagtggtg ttgatgctta ggtcctgcgc aatgaaagct catgaacccc gtcagatccg
     4321 gaaggaagca gcggtaagtg atcacttttg tgtgccgtgg gggtgcctaa gttgagctaa
     4381 ctgttttggt aacgcctatg gaattaagtc gatagatggt gtacagcatt taatatataa
     4441 aagactaatc ccttagataa tgagggattt ttttattaat tttgctaaag agaggtaaaa
     4501 agatgcataa agcattatat agagcatata gaccacaaaa atttgaagat gttattggtc
     4561 aagatcatat tattaagact ttaaagaatc aaatatatag tgataatata ggacatgctt
     4621 atttgttttg tggaacaaga ggtactggta aaacatctac tgcaaagatt ttttcaagag
     4681 cagtaaactg tttgaataaa ataaatgaag aaccgtgtaa tgagtgtgag atatgcgaat
     4741 ctgtattaaa agataataca atggatgttg tagaaataga tgcagcttca aataatagtg
     4801 tagatgatat aagagagctt agagaaagtg tgaaatattc tccagccaat gctaaatata
     4861 aagtatatat aatagatgaa gttcatatgt tgtctcaagg agcttttaat gcattattaa
     4921 agaccttaga ggaacctcca tcatatgtaa tttttatatt agcaactaca gagcctcaca
     4981 aaatacctgc aaccatttta tcaagatgcc aaagatacga ttttaaaaga gttacagtta
     5041 aagatatgac ccttagaatg aaaaagattt gtgaagatga aggaattgat atagatgata
     5101 aagctcttaa tttaatagct agaaattctc aaggagctct tagagatgca cttagtatat
     5161 tagaccaatg tatgtctttt ggagaaagta agatagatta taaagatgtt gttgaattaa
     5221 tgggaagcgt aaatatagaa caattatttg aattgtctca atgtatagta gagcaggaca
     5281 ctaaaaagtc tcttgagata ttaaatgaat ttgtcttatg gggcaaagat ataagaaatc
     5341 ttataaatga cttaattgat cattttagaa atttaatggt atgtaaggtt tcatcagaat
     5401 tggatgaaat aatttcatta ccagaagaaa ctatagaaca acttaaaatt cagtctaaaa
     5461 atatagatat aaatgattta ataaggattt taaatatatt atcaataact caggatgata
     5521 taaagtcatc ttcaaatcca agggttttag tggaaataac cataatgaaa attgctcaac
     5581 caatgtttga tgaaagtaaa gaagctctta taaaaagagt tgagaactta gaaaaaatga
     5641 ttgaactggg gaactttaag tctgaaaaaa ttgggaataa taaagaaaaa gagtatgagg
     5701 tcgatagaga aatagatgta aaacaagaaa atgtagttta tgaagatgta aaaaatgagg
     5761 acgttatact tatagagtca tcttggaaga atatacttaa acaaattaaa aaagataaaa
     5821 aaatgccaat atacgcactc ttaagtgaag taaaaagttt taatgtatat tctaatatgt
     5881 tgtatgttat atttgatgat aaatttgatt ttgcaaaaac tagactgagc tctcaagata
     5941 caataaatta tttagaaaaa acaataagag atgttttaaa tagaagtttt aatgtaaaaa
     6001 tagtactgac atcagaagtt aaagatataa atcttgaagt taaagagaaa aaggacattg
     6061 gtgaggaaat attaaagaac atagtgagtg aagagatatt agaaataaaa gatagtattg
     6121 atgaaaatga gagtaaataa taaaatacta ttatgatata ataataaaat gaaaataagt
     6181 ttaggaggaa tagtgtttat ggctaaaaaa ggttttggag gcggaatgat gcctggtggt
     6241 ggaaatatga acaacttgtt aaagcaggct caaaaaatgc aagaaaatat gcaaaaagct
     6301 caacaagagt tagaaagtaa agaagtagaa gcttcagttg gtggaggagc agttactgta
     6361 aaagttaatg gtaaaaaaga agttatagat ataactatca agccagaagt agttgatcca
     6421 gatgatatag aaatgctaca agatttagtt ttaagtgcag taaatcaagc attgagaaat
     6481 attgatgata tacaagcaag tcaaatgagt aaggtaactg gaggtatgaa tatacctggg
     6541 ttattctagt aggtggtatt tatgcaagtt tatacaggtc ccataacgag acttatagag
     6601 gaattttcaa agcttcctgg agtaggaaga aagactgctc aaagattggc ttttcatatt
     6661 attaatatga atactaatga tgtggaagct ttatctaaag cgattataga tgcaaaaaga
     6721 gaaataagat attgctcaat ttgttgtaat ataacagata cagacccatg tagcatgtgt
     6781 tcaaataaaa gtagagattc aagtgttata tgtgttgtgg aagatccaag agatgtagca
     6841 gctatggaga gaacgagaga gtttaaagga cagtaccatg tattaaatgg agtaatttct
     6901 ccaatggatg gaattggacc agatatgtta aaaataaagg aacttatcca aagacttggc
     6961 aatcaggaag tcaaagaaat aataatggca acaaatccta ctattgaagg tgaagctaca
     7021 gccatgtata ttgctaggtt agtaaaacca atgggaatca aagtcactcg gatagctcat
     7081 ggtcttcctg ttggaggcga tttagagtat gcagatgagg tcacaatatc aaaagcacta
     7141 gaaggcagaa gagaaatata aaaatcgagg ttaatacctc gatttttgta attttttgag
     7201 ttaaattcaa attaattttg atttagattt attgaataaa tttcttctgt attgtataat
     7261 ttgttgtata aatcatttaa aaattttgat aggggtgcaa catgaaacca attatgaact
     7321 tagaagaaca taatgcaatg aaagaaaaaa gagttaaaaa aatgaaatca aaagataaac
     7381 ctaaatatga tgattttgag gatataagtt tttctaataa gaaaaaaact aaaaaaaatg
     7441 tacgaaataa aaatgctgaa tttagaaata attataaaaa ttatcagtat gattatgaag
     7501 aaga
//
LOCUS       DAELTA010000167         7480 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.168, whole genome shotgun sequence.
ACCESSION   DAELTA010000167 DAELTA010000000
VERSION     DAELTA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.168"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            111..1088
                     /locus_tag="KPG13_003091"
     CDS             111..1088
                     /locus_tag="KPG13_003091"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG0986783.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1081..2049
                     /locus_tag="KPG13_003092"
     CDS             1081..2049
                     /locus_tag="KPG13_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG0986784.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2232..2822
                     /locus_tag="KPG13_003093"
     CDS             2232..2822
                     /locus_tag="KPG13_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0986785.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2841..3611
                     /locus_tag="KPG13_003094"
     CDS             2841..3611
                     /locus_tag="KPG13_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG0986786.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3748..4716
                     /gene="dusB"
                     /locus_tag="KPG13_003095"
     CDS             3748..4716
                     /gene="dusB"
                     /locus_tag="KPG13_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG0986787.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4825..5304
                     /gene="greA"
                     /locus_tag="KPG13_003096"
     CDS             4825..5304
                     /gene="greA"
                     /locus_tag="KPG13_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG0986788.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5319..6848
                     /gene="lysS"
                     /locus_tag="KPG13_003097"
     CDS             5319..6848
                     /gene="lysS"
                     /locus_tag="KPG13_003097"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG0986789.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7157..7351
                     /locus_tag="KPG13_003098"
     CDS             7157..7351
                     /locus_tag="KPG13_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986790.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2988 a          755 c         1510 g         2227 t
ORIGIN      
        1 ataattaaaa aggaaattta aaattaatag tattaaaagt atattaataa tgtataatta
       61 tttattagaa gaaaataagt catgaaatta tgtcatatta gaggtgaaat atgagagata
      121 aaataataga gattatatta aataataaat ctgaatttat ttcaggagaa gaattatcaa
      181 aacaattagg ggtgtcacgt gcagctatat ggaaacatat gaaagcctta aaagaagagg
      241 gatataatat agaatctgta aataaaaaag ggtatcgatt agcggaaaat ccaactgatt
      301 tattaagccc acagaatatt tactataaat tagatacaga gtttattgga aaaaatattg
      361 aacatcttga cactatagat tcaacaaatg aacatgctaa aaggatagct ataaatagtg
      421 tagatgggac tgtgatttta agtgaacatc aagatatggg gaaaggacga ttgggaagaa
      481 gatgggattc aaaacctcat gaaggaatct ggatgagtat tatattaaaa ccagatatgg
      541 aaccattcaa agccccattt ataactttaa tagcaggagc aagtgtaacg aaagctttaa
      601 acaatctagg agcaaataca ttaataaaat ggccaaatga tgttatttta aatggaaaaa
      661 aaatatgtgg tatattaaca gagttatctg ctgaaattga aagaacaaat tatgttgttc
      721 ttggaattgg aataaatgtt aaaataatgg attttgaaca agaaatagct gacaaagcca
      781 catcattgta taaagaagga tatatgttat ccagagtaga tattgttagg aatgtattaa
      841 ctgaatttga aaaattatat ttagattata ctgtaaataa ttccaaagaa aaaaccttaa
      901 aaatatgtag agattattct gcaattatag ataaaaatat atatgtatta aaaggagata
      961 atagagaact agtgaaatgt ttagacataa atgaagaggg aaatttatta gttcaaagaa
     1021 gtgatggaag tataaaagaa attatttcag gtgaagtttc tataagagga gaaaaaggat
     1081 atgtataata atattttgga tatagaaggc ttaaaggttg ggcaggttga ggatagaaat
     1141 ggtcttactg ggtgcacagt agtaatatgt gaagaaggag ctacttgcgg tgttgatgta
     1201 aggggctctg ctccaggtac tagagaaacc gatttattag accctgtaaa tatggttcaa
     1261 aaagttcatg cagtagtttt ggctggaggt tctgcatatg gtcttgaatc cacttgtggt
     1321 gtaagtagat acttagaaga aaaaggaatt ggctttgatg taggtgtagg taaagtgcct
     1381 atagttgtag gagcagtttt atttgattta ggagtaggga gctttaaatg tagaccagat
     1441 ttaaagatgg gttatagagc ttgtgaagtt gcaaatactg atattttaaa tcaaggaaat
     1501 tatggagcag gttgtggagc cactgttgga aagataagag gtcaggaata tgctatgaaa
     1561 ggcggaatag gtagctattc tataaaatta gataatggtc taattgtatc tgcattaatt
     1621 gctgtaaatg ctcttggaga tgtttatgaa aatggtaagg ttatagctgg tgctttaaat
     1681 gataaaaaga cagaaacaat tgatacatat aaattaatga aacaaggtga aagtaaaggt
     1741 ggatttagtg tagataatac tacaattggg ataattgcaa caaatgctaa gttaaccaaa
     1801 gctggatgta aaaagatatc tcaaatggga catgatggat atgctaagtc tatttttcct
     1861 atccatacac ctcatgatgg agatacaata ttcacaatat caacaggaga aatagaagca
     1921 gatataacat tagtaggttc attggctgta gaggttgtgg aaaaaagtat aataaatgca
     1981 gtaaaaaaag cggatagtgt agaaaatata atggcatata aagatatagt aaaaaaacga
     2041 gaaatatgat gaaattagca tatttttctt gtcaaattgt tgacaatggc aactcatctt
     2101 gataaaatta ttcttgttag agcgggcatc ctacaatggt agcttgactc atagttataa
     2161 gaaaaaacga aaaaaattaa aaaattaagt agtccggcat cttaatgagc tggaacggag
     2221 gtgtattttt tatgatgaac acaagtgcat cagtaagaaa agtaaatgta agaaagatga
     2281 ctataatagg agtactatca gctatttcta ttatgctatc tatgactcca ttgggattta
     2341 ttccagttgg acctacaaaa gcaactataa tgcacatacc agttattatt ggagctatta
     2401 tggaaggacc aatagttggg gcagcaatag gatttatatt tgggatatca agtctcttaa
     2461 atgctataac aaacccaaca gcaacatctt ttgtttttat aaatccatta gtttcaatat
     2521 taccaagagt tatgataggt gtattagctt attatgtata tcagttaata attaaggcta
     2581 caaataaggt atacatatca ggtttaatta caggggcaat aggttctctg ttaaatacag
     2641 ctggagtttt aggaatgata tatgtcttat atgctgacaa gtatcttcat gcaatgggta
     2701 aaagtggttc agcaggaaaa gtaataatgg ctcttgccgc agcaaatggg gtgccagaag
     2761 caatagtagg ggctttagta gtagctgctg ttgccactgt acttaagaaa agcaaaaaat
     2821 agaaagaagg ttaatgacat atgcttctag tatttgatgt tggaaatact aatatggttt
     2881 taggtatata taaaggtgac aaattagtta attactggag aattaaaaca gatagggaaa
     2941 aaacgtctga tgaatatgga atcctgataa gtaacctatt tgattatgat aatgtgaata
     3001 taagtgatat tgatgatgtt ataatatcat ctgtagttcc gaatgttatg cattctcttg
     3061 aaaacttttg tataaagtac tgtaaaaaac agccattaat agtaggtcca ggcataaaaa
     3121 caggtctaaa tataaaatat gataatccaa aacaagttgg ggcagataga atagttaatg
     3181 ctgtagcagg gatagaaaag tatggagcac caagtatact tgttgatttt ggaacagcaa
     3241 ctacattttg tgctatctct gaaaaaggtg aatatttggg tggaacaata gcaccaggaa
     3301 taaaaatatc tagtgaggcg ttatttcaaa gtgcgtctaa attacctaga gtagaattag
     3361 ctaagccagg tatgactatt tgtaagagta ctgtatcagc catgcaatct ggaataattt
     3421 atggatatgt tggtttagtt gacaaaataa taagtatgat gaagaaagaa ttgaattgtg
     3481 atgatgttaa ggttatagct acaggtggat tagctaaact gattgcttca gagacgaaaa
     3541 gtatagatta tgtagatggt tttttaacac tagaaggatt gagaataata tatgaaaaaa
     3601 accaagaata attaatatac agggaattgg gttatatatc caattccctt tttatgatag
     3661 atttctagct atatgtggta tattataatg aggaaataag aatttaaagt taaatcacaa
     3721 aataaaggca aaaataggag aaaaacaatg aaaataggaa atttagaatt gaaaaataaa
     3781 gtttttctat cacctatggc aggtgtaaca gaccttccat ttagattaat ttgtaaagaa
     3841 caaggatgtg ggcttttata tacagaaatg ataaatggta aggcactttg ctatgatgat
     3901 gaaaatacta aaaaaatgct taagatagaa gaggaagaac atcctgtagc agttcagata
     3961 ttcggttcag aaccagagtt tatgggaaga gcaacagaga taatgaatga ttattcaaat
     4021 gagattctcg atataaatat ggggtgtcca gctccaaaag tagttaaaaa tggagatggt
     4081 tcagcactta tgaaaaatcc taagcttgca gaagaggtat tgagggcagt agtgaagaac
     4141 tcaaaaaagc cagtgacttt aaagattcga aaaggctggg atgataatag tgtaaatgct
     4201 gttgaaatag caaagatagc agaggattgt ggaataagtg ctttagcaat acatggaaga
     4261 actagagaac agttttatac aggcaaagca gactgggata taattgcaga gattaagaaa
     4321 aatttgagta tacctgttat aggaaatggt gatgtattta caatagaaga ttcaataaat
     4381 atgttagaca aaactggttg tgatgctata atgataggta ggggtgctca aggtaaccct
     4441 tggatattta aaagaataaa ccattatatg aatacaggcg agattttgcc agagccaact
     4501 cttaacgaaa aaataagtac tgccattaag catttaaaac ttgcagtaga agaacatgga
     4561 gagtatgtag ctgtaaggga aatgagaaaa cacatcgctt ggtatttaaa aggacttaga
     4621 aactctgcaa aattaaggga tgaaattaac aaaatagaag actatcaaga agttgtgtcg
     4681 aaattggagt actatatgga agactccttg acataaaaat aatagtgact tataatatag
     4741 cgcataaatt aaaaaaagat tataaaattt ttaaaatata attaggaaaa ttcctaagaa
     4801 atttgtgaag gagttgtatt tataatggaa gaaaataaag agtttttatt gacacaagag
     4861 ggatatgata aattagaaga agaattagaa aacttaaagg ttgtaaagag aaaagaagtt
     4921 gcagagagaa taaaagttgc tatatctttt ggtgatttat ctgaaaatgc tgagtatgat
     4981 gaagctaaaa aggaacaagc acaagttgaa gaaagaattt taaaattaga aaatatggtt
     5041 agaaaagctg ttatcataga tgaaagcaaa atagacctta atgttgttac aataggatca
     5101 atagttaaag ttaaggattt agaatttgat gaagaggttg aatatactat agttggttcg
     5161 acagaagcag acccatatga tggtaaaata tcaaacgaat ctcctgtagg gaaagcactt
     5221 ttaggtagag ctgcgaaaga agttgtggaa gtgcaagttc ctgatggagt tgctaagttt
     5281 gaaatattag aaataagaag ataatttgga ggaaaaaaat gaaaaataat caacaaagca
     5341 atgaagaagc gcaaattcaa gaagacctta gtgaagtact tcaagtaaga agagataaac
     5401 taaaaaaatt acaagagtca ggtagagatc cttttaaaga aagtagatat gatagaactc
     5461 attattctat ggatataaaa gataactttg attctcttga gggtaaaact acaaaaatag
     5521 caggacgtat aatgagcaag agaatacaag ggaaagctgg ctttatagat attcaagacc
     5581 aagagggaag aatccaatct tatgtaagac tagatgctat aggagaggaa gaatatagtg
     5641 tattttcaac atatgatatt ggagatatag ttggaattga aggagaaatc tttaaaacta
     5701 aaaaaggtga aatatctgta aaggctaaat ctgtagtatt actatgtaaa tctttacaag
     5761 ttttaccaga aaaatatcat ggattaaaag accaagaact aagatataga caaagatatg
     5821 ttgacttaat agtaaatcca gaagtaaaaa atgctttttt aattagaaca aaagcattaa
     5881 aagcattaag agcatactta gatgatagag gatttttaga agttgagact ccaatattaa
     5941 atactatagc aggtggggca aatgctagac catttataac taatcataat actttacata
     6001 taccaatgta tttaagaata gcgaatgaat tgtatttaaa aagacttatt gttggtggat
     6061 ttgataaagt ttatgaaatg ggaagaatgt ttagaaatga aggtatggat ttaaaacata
     6121 atcctgaata tactgcaata gaattatatc aagcatatgc agactatact gatatgatgg
     6181 aaataacaga aaatgttatt gcacatatgg ctgaagttgc tacaggaagt atgatagtta
     6241 actatcaagg aacagaaata aactttacac caccttggaa aagaatgagt atggaagatt
     6301 gtgttaaaga atattctggt gtagattttt ctactataaa tacagatgaa gaggcactag
     6361 aagttgctag agaaaaaggt atagagataa aacctggaat gagaagagga gaagtaataa
     6421 atgctttctt tgaagaattt ggagaagata aattaatcca accaacattt ataactcacc
     6481 atccagttga agtatctcca ttatctaaga gaaatgttga agacccaaga agaactgata
     6541 gatttgaggc atttgctaat aagtgggagt tagctaatgc attttctgaa cttaatgacc
     6601 ctattgacca aaaaggaaga tttatagacc aattgagaaa aagagaatta ggtgatgatg
     6661 aagcttttga aatggatgaa gatttcttaa aagcattaga ggtaggtcta cctcctacag
     6721 gaggactagg aataggtata gatagagtaa ttatgttatt aactaactct ccatctataa
     6781 gagatgtatt attattcccg actatgaaac ctattgataa caatccaaat aaagaagaag
     6841 aaaactaaga aatattagca ttctactaag ttttaaataa agtttagcta ttaaattatc
     6901 aataaatagc taaattttat ttaaaatgtc agtgtggaat gctttttata tgcttcaaag
     6961 attcttttag atatttaatt tataaattac caacaaacgt tagaaattga tattagtttt
     7021 tgttgattga gactttatat ttagaaaata tgattgaaag taatttatta tgaaaatatt
     7081 tagaataatt tatgagaagg taaaaagaat aatttatgag aatactatat ataaaaaatt
     7141 attgggggaa tgtctgatgt ttcaagtaat taatttttta tgtttatttt gtgttggatt
     7201 tttggttctt gatgttatga tatatttttg taaggatgca atggatattt ttgagggtac
     7261 aactaatgtt aataaagtta agagtataaa tgtttgggca gatagagtga gagctaattc
     7321 atattcttat gaaaataaaa ttgcaaaata ggtaaaatta tatatgcatg tattgtttgt
     7381 aatatcaagg gttatatcta ttagttttaa ttatatttat taaaaaaacc aaaagttata
     7441 acctttttta aaataaaaaa caaaaaaagt attgacgaaa
//
LOCUS       DAELTA010000168         7475 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.169, whole genome shotgun sequence.
ACCESSION   DAELTA010000168 DAELTA010000000
VERSION     DAELTA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.169"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(60..911)
                     /locus_tag="KPG13_003099"
     CDS             complement(60..911)
                     /locus_tag="KPG13_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG0986791.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(960..2129)
                     /locus_tag="KPG13_003100"
     CDS             complement(960..2129)
                     /locus_tag="KPG13_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0986792.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2600..3517
                     /locus_tag="KPG13_003101"
     CDS             2600..3517
                     /locus_tag="KPG13_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG0986793.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3646..4575)
                     /gene="pfkB"
                     /locus_tag="KPG13_003102"
     CDS             complement(3646..4575)
                     /gene="pfkB"
                     /locus_tag="KPG13_003102"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG0986794.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4844..5569
                     /locus_tag="KPG13_003103"
     CDS             4844..5569
                     /locus_tag="KPG13_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986795.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5652..6806
                     /gene="nagA"
                     /locus_tag="KPG13_003104"
     CDS             5652..6806
                     /gene="nagA"
                     /locus_tag="KPG13_003104"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG0986796.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
BASE COUNT         2640 a         1092 c          969 g         2774 t
ORIGIN      
        1 aatttttcat ataataaata tcctaaactt atctaaaata aatttaggat attttatttt
       61 tatactctat ttacagaccc acacacttta atcttttctt tcacaaaatc ctttactgat
      121 tgaagccctg ctaatccata ctttttaggg tctataacct cagaatcttc atctaaaact
      181 tttttaaccc ctttactata tgcaattctt aattcagttg catagttaac ttttgcaata
      241 cctcttttta tagcttcttt tataatatca tcatgtattc ctgaacctcc atgcaatact
      301 aaaggtattg aaactacatt tttaatttct gataacctat ctaaatctat tttaggttca
      361 cccttataca atccatgtgc agtacctata gcaatagcca atgaagatat tcctgttcta
      421 tcgacaaact cttttgcctc ttttggattt gtataatcat tactattccc gccatctaaa
      481 tcatcttctt ttccaccaac tttcccaagt tctgcttcca ctggtataag agaaggacta
      541 catgctttta ctactgattt agatatagat atattatttt caaagctttc atgagaacca
      601 tctatcatta ttgatgtata tccacttctt aaagctttca ttgcaaggtc aaaacttgaa
      661 ccatggtcca gatgcattgc tacaggtata tttactcttt ctgcagcaac ctttgcattt
      721 gctagataat aatcaagacc tgcatatttt acagtagatg gagtagtttg taatattact
      781 ggagaattta actcctcagc agcttctata acagcaatta ccatttccat attttctaca
      841 ttaaatgctc ctactgcata cccttcatct tgtgcctttt taagcattga tgttgttgtt
      901 actaatgcca cattgatacc ctcccaaatt atttagttat aatattttaa tcttatcttt
      961 taatattcat atattgttac accttttact actcgattta ctgttcctgt agctgatgga
     1021 gtatctggct tatttccaac ctttactgat acaaacaatg ctatagtttg tgcaaacaat
     1081 atgtatggca ttgcaagata cacatctggt aatttattat acttttcttt aaactcaaac
     1141 tttgttcctg agaagttatt ttcattttct actgacactg cacaagtgaa tttagcaatt
     1201 ttatcccttt ttatctcctc taaaacatct aaatcatatt ttcttgtata taaacagtta
     1261 gatacaaatt caaatacaag tgtattttca tcaataaatg attttggtcc gtgtctaaac
     1321 cccataggag aatcatacac tgtagatatt tttcctgctg taagttctaa aagttttagt
     1381 tgtgcttctt gtgtcaaacc acctaatcct cctgaaccta aatatacaac tctatcaaaa
     1441 tctttattta ttaactcatg tatttcatct tttctatcta taacatttct tcccatttca
     1501 attattgcat ttatatatga cttttctaca tcatcttcta aactatcgaa tattaacata
     1561 gcagataaca tcatacacga aaaacttcct gtcatggcaa agccttcatc atttgatttt
     1621 gatggcatta ataacaagta attattttcg tcgtttttag tcatcttagc cagttctcct
     1681 tctggattac aagttatagc taaatgataa aaattatcta caatcttatt ccctaaattt
     1741 aaagctgcta aactttcagg actatttcca cttcttgcaa atgaaattaa taatgttggt
     1801 atatcttttt ttaagtaatc atatgggttt gaaactatat ccgtagtagg tattgcttca
     1861 aatatgtatt ttcttatgtc atttttattt ttaaggtaag gaagtattga atttcctatg
     1921 taagctgaag ttccagcacc tgtaaatata actctaaact gcccatgatt attacttatt
     1981 gtatctatga atctacttaa tttttctttg ttagatttat atatttcata agtttctaac
     2041 cataattcag gctgttgttt tatttcatta gttgttatta ttgcacctaa atcttttagt
     2101 ttttgttctt ctaatttaaa cattttcatt ctcctttcat acttgtaacg tttacatttg
     2161 gtaatgaggt tataaccact ttaaactata aaaaaatata ctcttttata attttttgct
     2221 tagatttttt agtactactt aaactcacta taaaatatac actatagatt ttaattaatt
     2281 ttatagttta tctttaaaaa tttaataaaa tcatactcta tatactttag tatttttatt
     2341 ttacgataaa agcttgttca gaccaaagtg gtaattacca ctaatccaaa tataaattaa
     2401 tttgaatttt ttgtcaatac ataaaatatt tatatcaatc aattttatgg atatcctaca
     2461 aaattacttt atcaatattt cttatttcgc taatttactt atataagctt taaatttatt
     2521 ttttgataca aagtttacaa atttatgtgt ctgaattata atcaattctg taaatataaa
     2581 aaccttagga gaaagtatta tgaaaaatat aaaaatggga atgataggtc ttggctccat
     2641 agctcaaaag gcatatttac ctatattaac taagtctgag agatttgagt ttgtaggagc
     2701 ttttactcca aataaagtta aaagggaaaa agtctgtagt gattatagaa tcatgccatt
     2761 cgattctatt gaatccctag ctaaaaaatg tgattgtatt tttctacata gctctacaga
     2821 aacacactac gaaattataa aaatattatt aaatttaggt gttcatgtat atgtagacaa
     2881 acctcttgct tcaacagtaa gtcaaggaga agaacttatc gagttaagta cgaaaaaaaa
     2941 cttaaattta atggttggat tcaaccgtag attctgtcct atgtacaaag aaattaaaaa
     3001 taatgctaca gaaattgtat ctatcaatat atgtaaacat ggattaaatt ccttaagaaa
     3061 tgttagattc gacagtactc ttatagatga ctatatacat gttattgaca ctgctttatg
     3121 gttagcaaat gaagatgttg aaattagtgg agaggattta tttttaacag acaataagaa
     3181 tcttatattt gtaagtcata aacttaaagg taaaaatttc tctataaaca cctctatgca
     3241 tagagattct ggaacaaagt tagaacaagt tgaaattctt tcaaagggta agattcaaag
     3301 agttaagaat cttaatgtac tggaaataga agaaggtgga aacctaactt taaagcaaag
     3361 tggtgcatgg gtaaatatat taaaacaaaa gggttttgag gatatatcaa atcattttat
     3421 tgattgtata gaaaataata taaaaccagc tattaatgga gaagagtgta tcaaagctca
     3481 aagattacta gagaaaatta taaattcagt aaaatagata tttattataa aaataggtta
     3541 aaaatgaatt tttctaacct atttttatat aagactatat cttttatatt aaaattaaaa
     3601 tctattacaa caacattaaa tttattatat tcccattaaa tgtatttaac ttaacttttt
     3661 tacttcaact aaatcttgat atttatcaat ttctcccaaa tctacaaatc cagtttccat
     3721 atttaaagca ttacttatac cacaagcatt tgcaaattta agtaaatttt ctattttata
     3781 accccttaaa atacctacac taaatccagc cactgtgcta tctccactac ctacagtatt
     3841 tatacacttt acaactggta cttttacatc atatacatta tcttcgttta aatacaccat
     3901 accatctttt cctaaagata tacatacatt ctctgcaccc atttcaataa gtttttttcc
     3961 tgacaagata atttcctctc ttgattctat attcattcct aataaatgtc ttatttcatc
     4021 tatatttggt tttattaaat atggttttga ttttaaagct atttttaatg cttctcctga
     4081 tgtatctaat ataaacttta tattatttgc tctacatata tttcctatct tttcataata
     4141 gtctatcggc atattctcac aataactacc agaagctacc agtattttag tattttttag
     4201 tacttcctct atatcatttt caaatctctc taaatcgctt ttttctacta ttggtccttt
     4261 ttctaaaaat tctgtactaa ctttattgtc ctcaattatg tttaagcaat ttctagtttc
     4321 ctgctctatt tttgtaaatt tattttgtat atctagtttt ctaagttcat tttttataaa
     4381 ttctccatta aatccgccta gaaatcccat agctaaagga ttttctttta aaattttaca
     4441 taccttagct acgtttaatc ctttacctcc agcagttgca ttcttactta ctaccctttg
     4501 cacctcacct atgtttatat tattcactct atacattcta tctatagatg ggttgaatgt
     4561 tattactgta atcatactag attctcctca ttattaattt ttacttttct aaacaataaa
     4621 ttaatctgta aaataaaaaa tacctcatct ataatatacc caaagtggtt attaccaatt
     4681 tgaatataaa ttattttact tgttttgtca atgtaccata attttataat aaattaataa
     4741 ccttacttat tgaattttga ttaaacttta ttcattgaca taaataattc tatattctat
     4801 aatcaaattg tggtaataac cacttagaaa ggactatatt tttatgataa aaagaaatga
     4861 taaaagacca atatatgacc agcttgttga gatattaaga actaagattg aaaatgagat
     4921 ggaaccaaac gatagaatgc tttccgaaag aaaaatttgt gatgaatatg gagtaagtag
     4981 aaccacagtt agactagcta tggctgagtt ggaacatatg gggtatatat acaaaagaca
     5041 tggaaaagga acttttgttg ctgcactaag tcaaaattct caaaacttaa tggaaagtta
     5101 tagttttaca gattatatga gaacacaagg aaaaaagcca ggtacaaagg tattatcatt
     5161 tgaggtagta gaaagtacaa agtatttctc tgaaaaatta ggtataactc ctggtgaaaa
     5221 aatgataaaa ataatgcgtt taagacttgc tgatgacctt cctatgatgc ttgaaagaac
     5281 atatcttcct atgaaagaat ttataggatt gacaaaagaa aaaatcgaac aaaaaccttt
     5341 atatgaaata tttagagaag attatggcga gattattaaa gttgctgatg aagaattttc
     5401 agctggaata atctctgata aggaagctaa gttattggaa gtaccaggag attcaccttg
     5461 tctgaaatta cttcgtacca catataatga tgacaatata gttattgaat ttactttgag
     5521 tgttgctaga agcgataaat ttgtatatag agtaagacat gtaagatagt aagttaaata
     5581 actttcaaga aaatagaatg atattataaa cgtcaatgcc acaatgatag tataattata
     5641 aggaggttat tttgaaaagt tttatttatg cagataaatt ttttatgaaa catggaatca
     5701 aagaaaaagg ttaccttagt ataattaatg gcaaattcgg tgaatttcaa caagaaagac
     5761 ctaatgttac tgataatgtt atagattttt caggtaaata tattgctcct ggcttagttg
     5821 atactcacat tcacggtctt ttaggtgcag atgtcatgga caatacattt gaagcaataa
     5881 acacaatttc aaaaggctta ttaaaatatg gagtaacatc tttcctacct acaactttaa
     5941 ctgattctgt agatacacta agtgattctg tagaaaatat aagcaatagc ttcaatcgag
     6001 ttgagggtgc aaaaatacaa ggtatttatt tagaaggtcc attttttaca gaaaaatata
     6061 agggcgctca aaatgaaaaa tattttaaag accctgacat caatatatta aaattatggc
     6121 aaaaaaaatc gaaaaattta ataagaaaaa tagcaattgc tccagaacgt aaaggttcaa
     6181 tagaattaac aagatatgca actaaaaata atatatcagt ttctcttggt catagttcag
     6241 ctacatttga gcaagttaaa caagtcgtta attcaggtgc taaagtgttt gtacatacat
     6301 ataatgggat gagtccttta aatcatagag aacctggaat ggttggggct gctatggtac
     6361 taaaagatac ttatgctgaa ttaatttgtg atggtcatca tgtaagccca atagctgtta
     6421 aaataatgat ggatgcgaag tctagtaata acatagcttt aattacagat tgtatgagag
     6481 ctggtgctat ggaggatgga aaatatactc ttggtaagtt taatgtaaat gtaaaaaata
     6541 gtgttgcaag attaaatagt gggagtttag ctggaagtgt attaactatg gacaaggctg
     6601 tgagaaatat agttgattgg aacatatcaa ctttagaaga tgcaataaaa atggctacat
     6661 atgtccctgc tttaagttgt aatattgata atgtctgtgg ctctatatat caaggtatgg
     6721 atgcagactt tatagtaaca gataatgact ttaatataca cgaaacttat ataaatggta
     6781 tctgtaagta caaagttaat aaataggttc aatataaggt tatggaggct gtctaaaaag
     6841 agtctggaaa tatttaaagt tatttctcca aaatctgatt taacactttt atttccatat
     6901 cgttaataaa ctattgcgat agaggttatt ttaaacaaat atatattata agtatttttg
     6961 tgataatatt ttcgataata tgtacattta aagtaacttt tctattatta tttgtaagtt
     7021 tttatcttga gcaaacatat aaatttttaa taaagcatga gataaaccct atatttcaat
     7081 tactaatgat gagattttga ttgataacag atatattttc aaatgtaaaa atactaaaaa
     7141 tcatatcaat tttaaagata ttgtaggtgt aaataaacaa gataatacta tagaaatttt
     7201 tacaaatgaa agcacttatt gtatatatct aaaagtttta agcattgaga gcgaagaata
     7261 tctaatagat agactaaaat cattttgttg attttatata ttttatgtta aatataaagc
     7321 agagtagtta aaactaccct gcttttgtaa ttaaaattta aaaataataa ctcataatac
     7381 attgaatttg agcttaacag taactattgt aataatagta tcaatctttt gtgttgaata
     7441 taatttattt acactatagt taagcatgat ttcta
//
LOCUS       DAELTA010000169         7456 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.170, whole genome shotgun sequence.
ACCESSION   DAELTA010000169 DAELTA010000000
VERSION     DAELTA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.170"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..514
                     /locus_tag="KPG13_003105"
     CDS             <1..514
                     /locus_tag="KPG13_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG0986797.1"
                     /translation="LEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNI
                     FFSWSLVCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYAL
                     RDNLAICFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDT
                     FILEKKKKSK"
     gene            complement(605..2056)
                     /locus_tag="KPG13_003106"
     CDS             complement(605..2056)
                     /locus_tag="KPG13_003106"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG0986798.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2072..2512)
                     /locus_tag="KPG13_003107"
     CDS             complement(2072..2512)
                     /locus_tag="KPG13_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0986799.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            complement(2760..3188)
                     /gene="ytfJ"
                     /locus_tag="KPG13_003108"
     CDS             complement(2760..3188)
                     /gene="ytfJ"
                     /locus_tag="KPG13_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG0986800.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(3142..3771)
                     /locus_tag="KPG13_003109"
     CDS             complement(3142..3771)
                     /locus_tag="KPG13_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG0986801.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            complement(3933..4472)
                     /gene="scpB"
                     /locus_tag="KPG13_003110"
     CDS             complement(3933..4472)
                     /gene="scpB"
                     /locus_tag="KPG13_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG0986802.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(4474..5193)
                     /locus_tag="KPG13_003111"
     CDS             complement(4474..5193)
                     /locus_tag="KPG13_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG0986803.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(5223..5819)
                     /locus_tag="KPG13_003112"
     CDS             complement(5223..5819)
                     /locus_tag="KPG13_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG0986804.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            6117..6953
                     /locus_tag="KPG13_003113"
     CDS             6117..6953
                     /locus_tag="KPG13_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0986805.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            6974..7396
                     /locus_tag="KPG13_003114"
     CDS             6974..7396
                     /locus_tag="KPG13_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG0986806.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
BASE COUNT         2528 a         1193 c          755 g         2980 t
ORIGIN      
        1 tctagagcaa ttattactta tttcttcagg taattatagt actctaaact ctttaccaat
       61 tggaatcaat tatatttgta tatatctgtg tatagccata ctaattttta agcagtatca
      121 tctttttaat atattttttt cttggagttt agtttgttca gttggagaac tcattttttc
      181 taaaaacttg ggctatgaat ttcctagctt aatatatttt atctttatct tctctaagtg
      241 cttgataatt tatgcagata tatatatggt tgatgttaga aaatttagag ttaacagata
      301 tgcacttaga gataacttag ctatatgttt tatttatttt tcattcatat ttttactaaa
      361 cacatttacc aactctaggt actattatgg ttttttaagt catagcacaa ctgctatttt
      421 tacgtttatt tttgttacta gtattatgta catacctgct cttttattta atagagatac
      481 ttttatactt gaaaaaaaga aaaagagtaa ataatttatt taaataaaaa ataaagccac
      541 atagataatc ttaaattaca aaaatatcca cgtggcttta taaattatat tttataattg
      601 ttttttagat ttctgccaaa tagtacaatg ttctaacagg ttctcctgta gccccctttg
      661 ctttgtatcc aattgatttt gatgatgtac ttgtccccgc tatatccata tgtatccaag
      721 gagtattccc aacaaattca ccaataaaag ctcctgctgt tatacatcca gcatttttac
      781 caccagtgtt tttaaggtct gcttcttctc cttttatcat atctttaaat tcatcataaa
      841 ttggcatctt ccataccctt tcccctgata aagtggcagc tttttctaat tcacaataaa
      901 aatcatcatt atttgttact ataggagttg cgacattacc taacaatatt aaagcagcac
      961 cagttaatgt agctacatca accacctttg ttactttttc attgtttata atgtagtata
     1021 ttgcatcagc taatgtaagt ctaccttctg catccgtatt taaaacttca atagttttct
     1081 ttgccataga actaattata tctccaggtt tgtatgaacc tcctgataaa gcattctcac
     1141 atgctgccac tactgctata acatttttct ttaatttact ttgagatata gcacacatag
     1201 cacctataac actcgcagca ccagccatat ctgtcttcat atctagcata gaagcatttg
     1261 attttaaaga atatccacca gtatcataag tcaatccttt acctactaga cctaatatat
     1321 ctgtactttc tttatctcca aagtatctca ttactatgaa tcttggttta ttaattgaac
     1381 ctcttgaaac agcgaaaaaa gcatccattc ccaatgcctt tattttttct tcttcataaa
     1441 catcaacatt aaacccaaac tctgcaccaa gtttaactgt ttctactgct aatgtttttg
     1501 gatatattac gtttgatggt tcattaacta gatttctagc tattatagtt gaattagcta
     1561 atattttagc cttttctatt tgacactcaa cttctttttc agtaaatata gttatagtat
     1621 caacttcttt tctttcaaaa tccttacttt ttgtcttata tttatcaaac ttataatttc
     1681 caagtaatat cccttctaca aaacaaccta tttcatcact atttaaatta agattcagta
     1741 cattaacatc actactctta tatgcttcat ttttcaatat attttttaat tttttacaca
     1801 aattcccacc tatgattcta attttttctc tattcaataa ctctctctta ccaaaaccaa
     1861 caagacaaac atttaccaaa ttgttatttt caaccaatgt tacagtatct atactatttt
     1921 ctttagaatt aaaatttctg cttatcatat ttattttatc aaatgtcgta gaaacatctt
     1981 ctccctcaaa tagagctact actgttatag cattttctaa actttttaat atttctttag
     2041 aatactttat attcataata aattctcctt ttcaaaataa ttgttctatt atatttttag
     2101 actcttccaa agaagtaaat aatttatctt tattatttat atctaggcaa tccattttat
     2161 cacttattat agatacaaac tttagtattt cattatccaa tatcttttct ccatttttag
     2221 ttattccata aaaagtattt cgtctatctc tactatcttc ttccctatat acatactctt
     2281 gctcaaccaa tttattaagt agtatacaca gactagaagt agatacatgc aaatctctac
     2341 ttagattttt aagctctatt ttatcatgtt ttcttatttt tactaaggca aagaactgtc
     2401 tttctgtaag aaccttatct tttttatatc tttcgttatt aataatcttt attaaattat
     2461 gaaaattatt catactttct aacaacaaaa tagataattc tttatcgctc ataacattca
     2521 cctcaaaact aatgatttat aactccctct tatatctcaa atgcgtttat atatctataa
     2581 tataatactt cttttctttt tataattata ctatatatca actttattat ctatttaaat
     2641 tgttttaaac taaaatctta ttagatataa atgtttaaag tacacaacaa aatagtggga
     2701 tttatattaa ccccactact ttacttaaaa ctatatacat atttaaaaat attttattct
     2761 tacatattgt ttttatcttg attcttatta ctttgtgata tattttgaat tttctccaat
     2821 actctaggag ttatagataa tatattatcc actagattga tattactatc taaactcatt
     2881 attctagtct ctccactttc aactactaca aatgctactg gttgtacaga tatagcccct
     2941 gcgctacctc cagcaaaatt cgtatctgat ttttcatttt ttaattcaat ttccctatct
     3001 ttatcatcat atccttttga gtattctcct cctcctatac caaagccaat tgtaaccttt
     3061 gatataggta caaccactgt agtatctgtt tttatagggt ctcctataat agtatttgca
     3121 tctatagaac ctttaattgt ttcaagagtt gtttccataa gactctgtat cgaacctgtt
     3181 gctctcatta ctatcacctt ccttatttcc tttattttta ttcataattt tattaatcat
     3241 tattataata ttaaataaag cttttattct tggccttata tgtatcctta tatttcctaa
     3301 aacataatct tttgtgaaat ctggatttac atttaaatac attttttctg catcaatcat
     3361 attaaataaa tttccataca aagtatttat caatacatat acagaagatg ttaagccaat
     3421 atttccagtt ccaaaattaa tatttgaata aagttcatgt atttttatct tttttaataa
     3481 tctataaatg ctcaaaaggt catcagctaa taatatactt gaatctatat tattcttcat
     3541 cccctctttt ttatcattat ttttactatt ttcagctgga taaagttgtc tatttatatt
     3601 tattaaatta aacatgtatt ttatattaag ctttaaacaa atatctttgt tttttatatc
     3661 agctattatt aagatgttta tatgagatat taaaatcaac atagttacac ttataataaa
     3721 aattacagct attattaaat acttaaaatt aaataattca ttaagctcca tgtttatatt
     3781 atcatcaaaa aattactatt tatacttaat tatccatatt tattttaaga gaattataat
     3841 tttatattta tacaaaaaaa cacaacaact tagagcattt taaatctttt aaaatttaaa
     3901 attacatact aaattattgt gattttatat ttttattcat ttgttgccac ttcttgataa
     3961 ttttctatag gtggcaattc ttcaagactt tctatattca acaactttaa aaattcatct
     4021 gttgttttat aaattattgg tttccctatc ttattcaatc taccagcttc tcttataagt
     4081 tcattttcaa acaaagtttg taatacctta tcacatttta ctcctcttat atcctctatt
     4141 tctacctttg ttattggttg tttgtaggca attatagtaa gagtttctaa tgtagcttgg
     4201 ctcaaacttt tctttttctt tggttcaata atcttcttta tatattctgc atagtcttta
     4261 tttgtacaca tttggtactt attctctaac ttgattattt gaatacctct attttgttct
     4321 ctgtactcct caataagtaa atttaacata atctctattt cttttgatga aagttcttta
     4381 tttattatat agtttaattc ttttatactt attggttcac catatgcaaa cataacagat
     4441 tctataatat actttatatc ttctcttttc attttaacac tccgaacttt ttcttattag
     4501 aatatcatca aaaaatatat cttgtactac gactatttct ttttctttta taagctctaa
     4561 tatagataaa aaagtagcta ttatttcatc attttcatat gattttataa tatttgtaaa
     4621 agtaacttca atcttctctt ttataatatc tcttatatat aatatttttt cttctacaga
     4681 tattattttc ttccttacta ttttatctag tttctcatca ttagcatttt ctatctgtga
     4741 tgttttaact ttaaatatgt atggaagtat attttttatt gcttctattg atatatcttc
     4801 taaatccaca ttgtcatcaa taattatctc ttctttattt ctataaaatc tctcattaac
     4861 ataagttata ttttctttta tatcttgtga tgctacctta aactttctat attcttctaa
     4921 cttttccata agttctattc ttgggtcttc ttcttcatct ttttgcttat aaagcagata
     4981 tttagacttt atctctaaaa gttttgatgc catagttata aattcactag ttatctctaa
     5041 atccatttta tctagcattt tcaaataatt tagatactgc ttagttatat ctattatgga
     5101 tatatccttt atatctattt tatgctttgt tattaaatca taaagtaaat ctaatggtcc
     5161 ctcatagact tgtaattgta tattatactt catatcaatc cctctttaat ctgtttatta
     5221 tattaaacaa ataaatatac tatttttagc aacactgcat atattggtgt taatattatt
     5281 ctatacatac cagtaaataa agcaattaat aaaattatat aactcatgct ttcaaattta
     5341 tacaaatatt cattccattt atatggtaca aatgtagata taactcccca tccatcaagt
     5401 ggaggtatcg gaagtaagtt aaaagctgca aatccaatac tatataaaaa cacatattca
     5461 gctattgtat ttatagcata catatgcaca tacttattga taattacaca cagtatagca
     5521 gtcaaaacat tacaaaaaac gcctgcaagt gaaactatca ggtttcctat cttatagttt
     5581 ttaaagttat ttggatttac tggtactggc tttgcccacc ctatcttaag gataaacata
     5641 gccaaaagac ctattgggtc aacatgtctt acaggattta atgtcattct accataagct
     5701 ttagctgtat catctcccaa taagtgagct gaataagcat ggccaaactc atgtattgat
     5761 attgccatag ctataccaac taaacttgct actatttctc ctatattcat acttaacatt
     5821 aatgtacatc tcctaaatta aatttttaat attattataa caattaaata gcttataatc
     5881 aaaattattt tacattataa taattttaac ttctaggcat aaaattaaaa tttgcttttt
     5941 attttataac ttttattatt atatcatatt cattgtgaaa atataataat tacacctact
     6001 gtaagtgtaa tattatattg acatgaattg acattattgt ctcttagtca atattataca
     6061 agaagtataa aaaaatatgt gttacacttt gtataacatg aaaggaggtt acctaagtga
     6121 gacaagaaga acgcacagta tgctttgata gggaattaaa aattgaagca tatagattta
     6181 aaggcataat gcaaaaattc ccaaaccatt ttcatgaaca ttatgtaatt ggttttatag
     6241 aaaaaggtca aagatattta tcttgtaaaa ataaagagta taccacaagt actggagatt
     6301 tgctactttt taaccctttt gatagtcata cttgtgaaca aatagatgat aaagtacttg
     6361 attacagatg tattaatatt aagccagaaa taatgaaaaa aactgtcttt gaaattacag
     6421 gtaaaaatta tttaccaaaa tttaatcaac ctgttatatt tcgcagtgag ctagtacctc
     6481 tactacaaga attacactat ataatcatgg aagaagaatt ggattttaaa aaggaagagc
     6541 tttttttctt cctaattgaa caattaattg aagaacatac agagccaaat ttgcaatcta
     6601 acttggaaaa tacaaatata gaaattcaag cagtatgtga ttatctagaa aataattatg
     6661 ctgaacatat agtacttgac gaattaagca ctatagcagg tatgaataag tactctctac
     6721 ttcgtaattt tacgaaatta aaaggtatta caccatatag atacctagaa aatatacggg
     6781 taaacaaagc taaaaagctt ttagaaaaag gcgtagaacc tatagatgct gcgatacaaa
     6841 caggatttgt agaccaaagt cactttacaa acttttttaa gaattttatt ggtcttacac
     6901 caaagcagta tcaaaatata tttataaatg atgattcaaa caactttaaa taaggtaaaa
     6961 aggagaaata aaaatggaaa ctaaaaattc aaaatgtgtc atggtaatag acgaaaattt
     7021 accaatggga attatatcca atacagcagc tataatggga attacactag gaaaacatgc
     7081 cccagaaact gttggcccag atgtaattga caaaacaggt aatagccact taggaattat
     7141 tgacattcct gtacctattt taaaaggaaa caaagaaata ataaaagatt tacgtaaaaa
     7201 attatatact ttggaattta acgatttaac tgttgtagat ttttcagatg tagcacaaag
     7261 ttgcaatctt tatgaagaat ttacacaaaa gattgctagt gttccagaag atgagttaca
     7321 gtattttggg atagcaattt atgggaataa aaagaaagtg aataaactta ctggtagtat
     7381 gcctttactt agataattgt taaacaaccc tatatatgat attaaatcaa atgatatttt
     7441 aagatacata aacttt
//
LOCUS       DAELTA010000170         7402 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.171, whole genome shotgun sequence.
ACCESSION   DAELTA010000170 DAELTA010000000
VERSION     DAELTA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.171"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            9..1310
                     /locus_tag="KPG13_003115"
     CDS             9..1310
                     /locus_tag="KPG13_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG0986807.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1386..1661
                     /locus_tag="KPG13_003116"
     CDS             1386..1661
                     /locus_tag="KPG13_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0986808.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            2996..3226
                     /locus_tag="KPG13_003117"
     CDS             2996..3226
                     /locus_tag="KPG13_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0986809.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3219..3800
                     /locus_tag="KPG13_003118"
     CDS             3219..3800
                     /locus_tag="KPG13_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0986810.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4379..5056)
                     /locus_tag="KPG13_003119"
     CDS             complement(4379..5056)
                     /locus_tag="KPG13_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0986811.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5245..6609)
                     /locus_tag="KPG13_003120"
     CDS             complement(5245..6609)
                     /locus_tag="KPG13_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG0986812.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6737..7258
                     /locus_tag="KPG13_003121"
     CDS             6737..7258
                     /locus_tag="KPG13_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG0986813.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2869 a          859 c         1287 g         2387 t
ORIGIN      
        1 taatttatat gggtaaatca aaagatgttg aaatagagaa gtctatttta ggcacaattc
       61 ttttagacaa taaattaagc tataagctag atgaattaaa tgaaaacatg tttatgaatg
      121 atatatgtct tgagatattt aaaattatga aagaacttaa aaaagaaaac atagtgattg
      181 atgtggccac tgtgaaatca aagatagata gaaaatctct agcaataaaa acaagtgatg
      241 tgactaatct aattacttgg ggtcagaatt ttggtcttga tggtcatatt aaaattttaa
      301 aagaaaattt agctagaaga agcataaatc aaaactgcca aaacctttta cacagcctaa
      361 accttggaga aaatatcgat acttgtattt acaaatttga aagtaatata aaggagattt
      421 tggacaagga tacttatgaa aatgatgatg taaatagtat agcagggaag gtgcttgatt
      481 ttttagaaaa caaaaaagat attggattta aatttggtat aaaattactt gataccacta
      541 taggaggtct ttttaaagga gagctaacca caatagcagc taaatcgggg gttgggaaga
      601 ctgctttggc acttcaaata atgttaaatt catttaagca aggtaaaaaa actttattta
      661 taagtagaga aatgaccagt gagcaagtat ttatgagaaa tatatgcaga gtaactggtg
      721 taagtacaag gaatatgaaa tctaaagaaa tagatgaaaa tgattggaaa cttattgtaa
      781 atgcgatagg tgatttaagc gaaaataact taatttatat caatgataag atagatacaa
      841 ttagtgcaat tagaaagagg ataaggcagg taaagccaga tttattaata gtggattatg
      901 tgcaactcct tacatctcag aaaagcatgg ataagagaga aagggaggta gctacttttt
      961 cgagagaact taaaaatatg actttagatt ttaatatacc tgtaatccaa ttaagtcagt
     1021 taaatgatga aatgaaagat tcaagacctt atggagatag acctatgagg gatagcaaag
     1081 ccatctacca cgatagcaac aatgtggttt atatacatca attaaagggg agtgattatg
     1141 aagaagcagt gagagatatt ggagaaagtg aggaggcagt tagagcatca gaatacaggg
     1201 gaattaagat ggttgattta atagttgcga agtgtagaga tggacagact aggcataagc
     1261 atttttgtta ctttggggat aagttacatt ttcaagaact aaattattag gtcaataaag
     1321 tagtaataac aagcttatta aaagtcggga aatagcaaat agattataaa atatgaggtg
     1381 aaaatatgtt ggaattaaga aaaattatag aattacttga agaacacaag atagaagtaa
     1441 gtattccaaa ggagttacat aagtatagtt tattggaata ccttgaattg tgtcaacaaa
     1501 gtgggggaac atatttttat attaaaacac tggaaggggc tttaaaagag gctagaaatg
     1561 agcttatgat taaattgttt aatgggaaaa attatatgga attagcgagg atgtttgaaa
     1621 tgagtgttat taatgtcagg catattgtta gaaaaaaata gatatttttg gagaatacaa
     1681 gtgtgatata cttctgaaat agaagttatt ataagaggag tataagtgtg attaacaaca
     1741 tgattttgtg aataaaattg aggatatata tgagttaaat gtgtgattaa taaggtaaga
     1801 ttgtgtataa gattatgagt atttaaaagg aggggctata attaatagtg tagagttgtg
     1861 tgtttgctta agattatata ataatttaat agattaaact atactaataa ggtaaaatcg
     1921 tgtgtttagt taaagcttat tataaaatct tgatatgtga ttaatgatgc aatatgtgaa
     1981 taaagttaag attataatag atttaacctg tggctaatag aataaaattg tgaataaagt
     2041 taagattata ataggtttaa attgtgacta atagagtaag agtgtaaata gagataaact
     2101 tcaatatgag cttaacctat gattaataga gtaagaatgt aaatagagat aaacttcaat
     2161 atgagtttaa cttgtggcta atagagtaag agtgtaaata gagataaact tcaatatggg
     2221 tttaacttgt ggttaataga gtaaggtgta actaaagata aacttcaata tgagcttaac
     2281 ctatgattaa tagagtaaga atgtaaatag agataaactt caatatgagt ttaacttgtg
     2341 gctaatagag taagagtgta actaaagata aacttcaata tgggtttaac ttgtggttaa
     2401 tagagtaaga atgtaaatag agataaactt caatatgagc ttaacttgtg attaatagag
     2461 taagagtgta aatagagata aacttcaata tgggattaac ttgtgattaa tagagtaaga
     2521 gtgtaactaa agataaactt caatatgagc ttaacctatg attaatagag taagaatgta
     2581 aatagagata aacttcaata tgggtttaac ctgtggttaa tagagtaggg tgtaactaaa
     2641 gataaacttc aatatgagtt taacttgtgg ctaatagaat aagattgtga ataaagttat
     2701 agttttagat gggtattaac ctgttactaa tagaatatga ttaggtgtaa aattatagag
     2761 ctgcataaga aaatattata gatatattaa atattagata ataaatattt atatagatac
     2821 acaaaagaaa aggcgatatg aagctaaact taatgacttc atatcgtttt ttatttgaaa
     2881 ctcctttgta tttagagtgt gatttttaat attgaggttt tatgtgatta agtcacagat
     2941 aaaaagtaag tatcttgata taattcttat agattaataa tatagggagg ttattatgtc
     3001 taataaaaaa aagaagtatg caaatatttt agcaaatgag tgcgttgctt gtggtagctg
     3061 tataaaagca tgtccaagaa gtgctatctc agttccatgt ggcattagtg caaaaattaa
     3121 tagagattta tgtgttggat gtgggatttg tgaaaagata tgtccagcat ctgtaattga
     3181 gattataaca atattaaaag aggaagaagg taagtgtcat gaataataaa aaacattggt
     3241 atgactattt atggattttt tcttcaatat atttgatatt gggatttgta aatattattt
     3301 tcgcttggat tgggctaatt tgtttctttg ttccactagc tatttctatt gttaagggaa
     3361 ataaagctta ctgtaataag tattgtggaa gagggcaact gtttaatttg cttggaaata
     3421 aattgaagct gtcaagaaat agagatattc ctaaatttat tagaagtaag tggtttagat
     3481 atgggttttt gacatttttt atggtaatgt ttgccaatat gcttttttca acttatttag
     3541 tgtttgaagg aagtagaaat tttaaagaag tagtgacttt gttgtggact ataaaaatgc
     3601 cttggcaatg ggcgtatcat ggaactttag tttcaccttg ggtggtacag tttgcatttg
     3661 gtttttatag tgttatgctt acatcaactg ttcttggttt gattactatg atattgttta
     3721 aacctcgttc atggtgtgtt tattgcccta tgggtactat gactcagatg atatgtaagg
     3781 caaagagtaa gtctgtatag aaaggcctta tgtgatattt acagttttgt tcaagtagag
     3841 atgagtagta ggtttgaata tatatgacaa atatatgagt ataggggcat agatttagtt
     3901 tgtgtaaggt aaactgattt atgcttcttt tttatgtctt agattattgg ctaagtggaa
     3961 acaatgaatt tagatattaa gaattattca gttatttgaa gaaaatagaa taataattaa
     4021 cattatatta atggatattc attacatata agccagttaa tagcaagatt tatttaatat
     4081 gatataggtc aagtatttaa actaaataag gtcatgacaa ttaatataag tatagatata
     4141 aaaagattag aagttataac ttataaaagg tattctatat aaatagtatg gggaaaatag
     4201 tcaaattaac ttattagaaa caaaaaatat acataaaaaa taaatgtaaa tatcaaataa
     4261 gatactatat caaaatagat ataagtatct tttttattat tgaaattatg aaaatatatt
     4321 tgattttaca caaaaaagag tgcctcatga actaaaaagt tcattttgag acacactttt
     4381 acagtgtcaa agaacgaagt ttatccttat ccaatacctt tataccacca cgagataaag
     4441 acacaatacc ttctctagca aaatatttca acattctcga tacaacttct ctggcactac
     4501 ccatatactt agcaatttgc tcatgagtca tatttatagt gctactgcca tttttagcta
     4561 tctcatcaat taaaaaagat gccaaacgct tatcaaaact cataaaaaga atttgttgca
     4621 tagcccacat aacatcagaa aaacgttcag tagctaattt atatgaaaat aactctgcat
     4681 gaatattttc tgtagataat tttgcaaaaa cagaggaact aatctgtata atatcgcagt
     4741 ccgtttcagc atctacatag acatcaaaag ttattgtttt tagaatacag gaagctgata
     4801 aaatacatac atctccatca tcaagtcgga acagtgtaac ctctcgacct tcatctgaaa
     4861 gtatgtatgt acgcactgtt ccagatttta taatcaaaat accaatacag tcactatcac
     4921 cacaatgtac tggattccct tttttgtaat gcgaggtatt tgctgaagaa attaataatt
     4981 ctttttgtaa gtcagtaagt ttatcccaaa aaggcaagct atgtgtaagt atatcaattt
     5041 cattttgtga aatcatacaa tatctccttt cttaaattgt tataatccca tgatacaagt
     5101 gaatagataa attgacaagt agtttgttaa gaggattatt tagtcagata gtaatagtat
     5161 gatttatgta gaaaaaatat gataatatat attcttgcta ttaatgagaa agtaatatgt
     5221 tttgatgata gtaggtttgt ataactaatg cataccgttt atttttaaaa gattaagttc
     5281 cttatgtaac cttatagaca taaaaccagc aattatagca gccatcaaat cagctattgg
     5341 ttgtgcatat aagataccat ttaacccata gaatacagga ataatcaaaa ttatagggat
     5401 aaaacagaca ccttgtctac acatacctaa aaatccacct tcctttgctt ttcctagtgc
     5461 gaggaataat gtagaataca ctgtatagaa tccaaatagc ataaatgaaa acccatttgc
     5521 cctaagagct ttactaccat tttctattaa taaaatgtca ccacttgtaa attgtgaaat
     5581 tatagatttt gggaagagta tcaatataag accaagcact acacaaaata ctgtagtcca
     5641 taatatggaa gtttttattg ctatatgtaa tctatcgtag ttctttgaac caaaattata
     5701 accagcaatt ggttggaaac cttttataaa accaaataca acaagactac ctaaagacgt
     5761 tattcgtgtt acagcaccca ttccagctat cacagaatca ccatactttg tagcctccat
     5821 attagttaat gtaatagcaa gactagtcaa taattgaaat attaaggtag gtatgccaat
     5881 ttttaggatt tctgaaaaaa tttcttttga aaaacagcat tcgttaattc taaaactaaa
     5941 tatacttttc ttgcttaaaa tatatcctac ataaacaagt gttgaagcaa tctgtgagat
     6001 agctgttgca atagaagcac cccttacccc cagaccaaaa gtataaatga atatcgggtc
     6061 taaaatcata ttaagcaaag cacctaataa catagcacac atagttgttt tagcagctcc
     6121 ttcacttgtg acaatattag tcatagtgac attgaatata ttaaaaatag atgacacgac
     6181 ataaatgcta gcataagata ttgcataagg cataatactt tttgtagctc ctaacaattt
     6241 taaaattgga ttgagaaaaa tcataatcac aataataact aatgtaccca ctataatact
     6301 actgtaaata gatgtactta ccacttttcc tgctttttca ttatcaccat ggcctaaaag
     6361 tctggcaaca tatgaagcag ctccatttcc aaacaataat cctaatccca caacaagctg
     6421 acctaatgga aatgcaacgg atatagctcc catttgactt gttccaagtc cacccacaaa
     6481 gtatgcatca accaaattgt ataatgcagt tatcatcata cctatcatgg taggtaatcc
     6541 catagataac aatgcttttg agattggggc agaccctaat aattcaattc ttttgttact
     6601 ttctttcata cttaacactc ctcttctatt gacattaagt ctaaattata atactataaa
     6661 ttatagtaag tatggaaaat agtactataa tttatagtat ttgtcaatag taagaaaact
     6721 aaaggagatg atttttatgg ctacaataga tttaattgta ttgggaatag taaagcaaca
     6781 acccaagagt gcttatgata ttcaaaaggc agtggaatac aggaatatat cacgttgggt
     6841 taaaataagt acaccatcaa tttataaaaa agtaattcaa ttagaacaaa agggatatat
     6901 tgaaagtaaa actgtgaaga atggaaaaat gccagaaaaa gcagtttatt cattaactga
     6961 attaggtgag acacattttt tagatttaat gtttgataca tctagcaaac caataaatat
     7021 tcttttagat tttaatgctg ttatagtaaa tttaaatagc cttacaccag aaaaacagaa
     7081 attatgtctc aataatattg aagagaatgt tcaagagtta aagaaaacta ttgaggaaaa
     7141 tataagtata aagacagaag taccagacac agcaaaagca gtgttggagc aacagttggt
     7201 gctagcacaa gcattggaaa cctggataaa ttctttaaaa gataatatgg gaaattagaa
     7261 ttagagaagc taacttaata atcattaaac aaaaaccagc atatttaata atgagatatt
     7321 agaaaaaata aaacaaatga tataaactga aaagtatatt taaaataaag ctcacttaat
     7381 tgagggagaa tgttaaactt tt
//
LOCUS       DAELTA010000171         7390 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.172, whole genome shotgun sequence.
ACCESSION   DAELTA010000171 DAELTA010000000
VERSION     DAELTA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.172"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..807
                     /locus_tag="KPG13_003122"
     CDS             <1..807
                     /locus_tag="KPG13_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HBG0986814.1"
                     /translation="ANFKKYIFKKNIIFNYTEFRDKVLFNGCVFLENCDFNRSIFSKN
                     YINDRIFEKVKFKGPDLVVNKVENFPRMDGIIFSMCTKFVLKNVEYGKSEYEHGKINY
                     RIARNQATKIGEYEMIGFYYYKERIYSSKIMKRSNYPTFSDYLVEKFFDQIARYTTGY
                     GEKPWNILLVIIAIISVFALLYLFVGIESSNSTLVALDINNIGDYSLSEIFKMYMDLW
                     YFSMATFSTVGYGDMVATSLIGKALAGIEVFFGVTIGAIWASVIIKRMIR"
     gene            911..2095
                     /locus_tag="KPG13_003123"
     CDS             911..2095
                     /locus_tag="KPG13_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG0986815.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2302..3015
                     /gene="rpsB"
                     /locus_tag="KPG13_003124"
     CDS             2302..3015
                     /gene="rpsB"
                     /locus_tag="KPG13_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG0986816.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3094..4005
                     /locus_tag="KPG13_003125"
     CDS             3094..4005
                     /locus_tag="KPG13_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG0986817.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4090..4794
                     /locus_tag="KPG13_003126"
     CDS             4090..4794
                     /locus_tag="KPG13_003126"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG0986818.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            4820..5377
                     /gene="frr"
                     /locus_tag="KPG13_003127"
     CDS             4820..5377
                     /gene="frr"
                     /locus_tag="KPG13_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG0986819.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            5380..5583
                     /locus_tag="KPG13_003128"
     CDS             5380..5583
                     /locus_tag="KPG13_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986820.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5612..6346
                     /locus_tag="KPG13_003129"
     CDS             5612..6346
                     /locus_tag="KPG13_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG0986821.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6359..7138
                     /locus_tag="KPG13_003130"
     CDS             6359..7138
                     /locus_tag="KPG13_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG0986822.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3039 a          761 c         1408 g         2182 t
ORIGIN      
        1 gcaaatttta aaaaatatat ttttaaaaag aatattattt ttaactatac agaatttaga
       61 gataaagttt tatttaatgg ttgtgtattc ttagaaaact gtgattttaa tagaagcata
      121 tttagtaaaa actatataaa tgaccgtata tttgaaaaag tgaaatttaa aggacctgat
      181 ttggtagtta ataaagtaga aaattttcca agaatggatg gaataatttt tagcatgtgt
      241 acaaaatttg tgttaaagaa tgttgaatat ggaaaaagtg agtatgagca tggcaagata
      301 aattatagaa tagctagaaa tcaagcaaca aaaattggag aatatgagat gataggattt
      361 tactattata aggaaagaat ttatagtagt aaaataatga aacgttctaa ttatcctact
      421 ttcagtgatt atttagttga aaaatttttt gaccaaatag cacgttacac aacaggttat
      481 ggagaaaagc catggaatat attattagtt attatagcaa taataagtgt ttttgcttta
      541 ttatacttat ttgtaggcat agaaagttca aatagcacac ttgtagcttt agatataaat
      601 aatataggtg attactcact aagtgagatt ttcaaaatgt atatggattt atggtatttt
      661 agcatggcta catttagtac agtaggatat ggagatatgg ttgctacaag tttgattggg
      721 aaagcattgg caggtataga agtatttttt ggagttacaa taggagcaat ttgggcatct
      781 gtgattatta aaagaatgat aaggtaatat ttgtgtaaat atggataggt attaattact
      841 agatataaat tggactaaat agggaaaatt taaatataaa tatacttaat aatccaagaa
      901 ggtgaaaaat ttgaaaaatg cactaaaaaa aattatatca ttagccatga ttctagtttt
      961 tataatacca aattattcat atgcagatga taaatctgta gacaagtatt caaaaacatc
     1021 tatacttata gaccaagaaa caggtagagt tttatatagt aaagaacctg atatgaaggt
     1081 accattagct agtttaagta agatgatgac ttttttactt gcaatagaag ctattgaaaa
     1141 taaagaagta aaagaaactg ataaagtaaa aatagacaaa tctattgcat cagtaaaagg
     1201 gtcaagttat aaacttaaag atggtgaaga agtgccactt atagagctta tgagaggtct
     1261 tatgattgta tcaggaaatg atgcggcaat tgctatagca aaacatattt gtaaaacgaa
     1321 agaagcattt gtagatagaa tgaataaaaa agcaaaagaa ataggaatga gtaatacaca
     1381 ttttctaaat ccaaatggtc ttccaatata cgatttaagc aaccccaaaa aaccagcaaa
     1441 agaaaatatc tctactgcaa aagatatagc tattcttgga aaatacatgt ttgaccatta
     1501 tgaaaaacaa gtaacagcta taactgatat ggagacatat acaaattcag atagaagttt
     1561 tgaaaaaagc aatacaaatg cattattaag aattatacca gaagtagatg gtataaaaac
     1621 tggatatact ggcaatgctg gatattgctt atcattttct atgattgtaa ataaaaatga
     1681 taaaaatgag aaaaaacgta gagtaatagg ggttgtatta ggtactaacc ataaaaataa
     1741 aagaacctct gcttcattag ctctactgaa atatggaaaa gaaaatttca atatgaaaaa
     1801 agtaattgat aaagatagct ttattggtaa gaaatatata aaaggaatga aagagttaga
     1861 ggttacatta aaagcaaaag atgaacttta tacaatatta aaagatgatg aaagtataaa
     1921 atcagaagtt aagattaaaa atatagagta ccctgttaaa aaaggtgata ctatgggtat
     1981 tataaaatat tatactaatt caggtgactt acttggaagt gtagatatag taagtaataa
     2041 tagtatagat aacgtatcat taaaaacaaa attaaaaatg aaatttgcaa attaaattca
     2101 aaaaatggta taaaatacac aaaaaaatat aaaaatagaa aatatggaaa aaatattgtt
     2161 atttcttcac ctctgtgata tactactaga ggtgaaaaaa taaaataaat cacacacagc
     2221 attgggatcc ttaaaaggtg cctattacat ggttttttag ggagaagatc aagctggagg
     2281 tataaaacca ggaggtaaga tatgtcagta atatcaatga aacaattatt agaagcaggt
     2341 gttcactttg gtcaccaaac aagaagatgg aaccctaaaa tggctaagta tatattcaca
     2401 gagagaaatg gaatatatat aatcgactta caaaaaacag ttaaaaaagt tgaagaagct
     2461 tacaagttca ctaaagaagt agctgaaaca ggaaagccaa tcttattcgt aggaactaaa
     2521 aaacaagctc aagatgcgat aaaagatgaa gctgaaagat gtggaatgta ctttgttaat
     2581 gaaagatggt taggtggaat gttaacaaac cacaaaacta taaaaacaag aataaataaa
     2641 ttaagagaat tagaaaaaat ggaagaagaa ggcgtattta atgttcttcc taaaaaagaa
     2701 gttataaaat taagagctga aaaagaaaaa ctagaaaaat acttaggtgg aataaaagac
     2761 atgcctgaat taccaggtgc aatgttcgta gttgacccaa gaaaagaaaa catagctatt
     2821 caagaagctc atagattagg tataccagta gttggtatag ttgatactaa ctgtgaccca
     2881 gaacaattag actttgctat accaggaaat gatgacgcta taagagccgt aaaattaata
     2941 actggtgcta tggcaacagc tgtaattgaa ggtagacaag gtgctgagga agaagtagct
     3001 gaagaccaag aataataatt tgacaattaa atggtaaggg tcgcccttac catttataat
     3061 atggaataga ataagtagga ggaaacttaa aaaatggcta atataactgc tcaaatggta
     3121 aaagagttaa gagaaagtac aggcgcagga atgatggact gcaagaaagc tttacaagaa
     3181 gctgaaggaa acatggaaaa agcagtagat ttattaagag aaaaaggatt atcaaaagct
     3241 gctaaaaaag ctggtagagt tgctgctgaa ggtctagttg caatagaaat gaatgatgat
     3301 aatacagttg cttctatggt agaagttaac tcagagacag atttcgttgc taaaaacgaa
     3361 gacttcaaag tgttcgttaa agatgctgct tgtatggcat tagctactga taaagaagat
     3421 atagcttctt tattaggtga aactcataaa gagggaataa ctttacaaga agttttaaat
     3481 aatagagttg caaaaatagg tgagaaatta gactttagaa gatttgctaa agttgtaact
     3541 aatggacaag ttgctggata cattcacggt ggtggaaaaa taggtgttct tgttgaaatg
     3601 gaaacagaag ctagagatgc taaagtttta gaattaggta aagatgtagc tatgcaagtt
     3661 gctgctatga atcctaagta tgtttcaaga gatgaagtag atgctgaata catagcacat
     3721 gaaacagaag tattaactca acaagcttta aatgaaggaa aaccagctaa tatagttgaa
     3781 aaaatggtta aaggaagatt agaaaaagaa ttaaaagagg tttgtttatt agagcaaact
     3841 ttcgttaaaa atccagatat aactgttaaa caattagttg ctgatgttgc taaggcagta
     3901 ggttctgata taaaagttgt taaagttgtg agattcgaag ttggtgaagg tatacaaaag
     3961 agagaagaaa actttgcaga agaagttgct aagcaactta agtaataacc aaagagagaa
     4021 cacgcaggtg ttctcttttt taaaaaatag aaaaaattgg atatgaagga ttgaaatagg
     4081 aggtttctga tggataaacc aatgtacaaa agggtattgt taaaattaag tggagaagca
     4141 ttagcaggag aaagaggttt tggtataaat aatgatgtag ttaatgatat tgctattgct
     4201 ataaaaaaaa tacaagaaat aggtgttgaa gtagctgtag tagtaggtgg aggaaacttc
     4261 tggagaggca gaaccagtga aggaatggac agaactactg ctgactatat aggaatgtta
     4321 gctacagtta tgaatgcaat ggcgctacag gatgcactag aaaatataga tgttgctaca
     4381 agagttcaaa ctgctataga catgagacaa attgcagaac catatataag aagaagagca
     4441 gttagacact tagaaaagga aagagttgtt atatttggtg caggaacagg aaacccttac
     4501 tttacaacag atacaactgc tgctttacgt gctgctgaga tggaagcaga agttatattg
     4561 ttagctaaaa atgttgatgc agtatatgac aaggatccaa aagtacatgc agatgctaag
     4621 aaatttacag aattaagtta catggaagtt atacagaaag aattaaaagt aatggattct
     4681 acagctacat ctttatgtat ggataataaa attccaataa aggtatttga gcttacaaca
     4741 gagaatataa taagagcagt aaaaggcgaa aatataggaa ctactgtaaa ataatgaaga
     4801 atgaaggagg agtaaaatta tgaaactgga aatacataag caattagaag agaaaatgaa
     4861 tgggacaatt gatgctttaa agtttgaatt tggaactata agagcaggaa gagctaatgc
     4921 acaaatgcta gacaaaataa gagttgatta ttatggaact ccaactccaa taaatcaaat
     4981 aggtgctata tctgtaccag aaccaagaat attaatgata tctccttggg ataaatctgc
     5041 tatgcatgaa atagaaaaag cgatagctaa ctcagactta ggattaaatc catcaaatga
     5101 tggtgaggtt ataagacttt ctgtacctgc tttaacagaa gaaagaagaa aagaattagc
     5161 taaaaaagct agtaaagctg cagaagaatt taaagttaga ataagaaatg aaagaagaga
     5221 cgccaatgag aaaattaaaa agatggaaaa aggcggagaa ttaactgaag atgaattaaa
     5281 aaaagcacaa gatgaagttc aaaaaatgac tgataaattt ataaaagaaa tagatgcatt
     5341 attatctaaa aaagaaaagg acataatgga ggtataatat tgaaaaaata ttataacctt
     5401 ttaggtcttc atgtagatga ggtaaaacaa tattttgatg agcaaaatat aaattatacc
     5461 gtaaaatcta ttgaaggtaa aaaggataaa gaaaaacttg tagttcctag agttataaaa
     5521 atatctgaga taggtgatag tgtagaactg ataatcacat acttttctga ctccttagtt
     5581 taaggagtca gaacaatatt ggaggcaaaa tatgaataac aacattatat atgatataga
     5641 cttaaataac atacctactc atattgctat tataatggat ggaaatggaa gatgggcaaa
     5701 agctagattt cttccaagaa ctgcaggaca taaggctgga gtagaaacta taagagatat
     5761 agtaaaagaa tgctcaaaat taggtgttaa gcacttaaca ttatatgcat tttcaacaga
     5821 aaactggaaa agaccgaaac ttgaagttga cacacttatg aacttattat ctacatactt
     5881 aagaaatgaa atagctgaac ttcaccaaaa caatgtaaaa gtaactgcta taggagatat
     5941 aagtgcttta ccaaaaactt gtataagaga attaaactca gctaaggaac tgacaaaaaa
     6001 caatacagga gttaacttaa atttagcatt aaactacgga agtagagcag atataaaaaa
     6061 tgctcttata gatatagtaa aaaattgtga aagtggtaaa atagatataa acaatattga
     6121 tgaagatatt ataaaaaact atttaagcac aaagtctata ccagatccag acttggtaat
     6181 aagaacaagt ggtgaacaaa gattgagtaa ctttttactt tgggaagttg cttattctga
     6241 gttttacttt actgatatac attggcctga ttttaaaaaa gaagaactac agaaggctat
     6301 atacgtatat caaagaaggg atagaagatt tggaggactt aagtaaggag attattttat
     6361 gcttacaaga attattgctt cactagctct ggttccatta tttctatttg ttgtatatgg
     6421 aggtattcct ctatatatag ctgagacagc aatagtttat attgcattgc atgagtttta
     6481 taaggcattt aaaataaaag atgtacatcc aatattcatt ataggatatt tattttctat
     6541 ttatttagct gtaaaaaata tttttaattt accattagaa tatacttatg cagtaatttt
     6601 tattttattt ttagcaagta taatttatat gttgatgggc aaaaacaatg ttatagatgt
     6661 ttcgattacg tttttaggag ttttttatat aggagtattt ttagatttta taattataac
     6721 tataaatgga tttgaaaaag gtagtatata tgtatggctt atatttgtga tatcttttat
     6781 gacagatata tttgcatatt ttagtggtta tttacttgga aaacataagc taattcctaa
     6841 agttagtcct aaaaagacta tagaaggagc tattggagga ataataggaa gtacactttg
     6901 ttgtatttta tttggatatt tatttggcat tgatttgctt caattagcga taataggaag
     6961 tataggaagt gtaatagctc aattaggaga tttatttgca tcttctataa agagatatgt
     7021 agggataaag gattatggaa agataatacc tggtcatggg ggtatattag atagatttga
     7081 tagtgttata ttagttgcac cttttgtata tagtgcgatt aaatttttta ttagataaag
     7141 attggttgga tttaaaagag caatctataa ttgctagtaa tgaaaatagc tatgtaactt
     7201 ttaaatgcag agatattctg cagttattag ttacatggct attttcattg ttcaaaaatt
     7261 tagaagacct tggggaattg tataagaata ggtattactt tataatagta tactatattt
     7321 gtagtgagta tattttttat actgtaacta tcattaaaaa atattttttt gtttaataat
     7381 ttcgatattt
//
LOCUS       DAELTA010000172         7343 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.173, whole genome shotgun sequence.
ACCESSION   DAELTA010000172 DAELTA010000000
VERSION     DAELTA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.173"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(162..1001)
                     /locus_tag="KPG13_003131"
     CDS             complement(162..1001)
                     /locus_tag="KPG13_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG0986823.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1025..1783)
                     /locus_tag="KPG13_003132"
     CDS             complement(1025..1783)
                     /locus_tag="KPG13_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0986824.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1830..2303)
                     /locus_tag="KPG13_003133"
     CDS             complement(1830..2303)
                     /locus_tag="KPG13_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG0986825.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2332..2748)
                     /locus_tag="KPG13_003134"
     CDS             complement(2332..2748)
                     /locus_tag="KPG13_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0986826.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2795..3847)
                     /locus_tag="KPG13_003135"
     CDS             complement(2795..3847)
                     /locus_tag="KPG13_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG0986827.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4243..4923
                     /locus_tag="KPG13_003136"
     CDS             4243..4923
                     /locus_tag="KPG13_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG0986828.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5056..5376)
                     /gene="sugE"
                     /locus_tag="KPG13_003137"
     CDS             complement(5056..5376)
                     /gene="sugE"
                     /locus_tag="KPG13_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG0986829.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5758..6585
                     /locus_tag="KPG13_003138"
     CDS             5758..6585
                     /locus_tag="KPG13_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG0986830.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2498 a         1319 c          846 g         2680 t
ORIGIN      
        1 actttttaaa atattaaata ctacatatag cttacataag tttaaaattt aatctattat
       61 aaaattaccc taattgccaa actttttaat ataaattttc caacatccta caaatataat
      121 caatagatat agaatcctaa cttacaccaa tatatattta tttaaaatag tcctaaaata
      181 cctgtaatag ctcccactac tatcagtata aacattattt ttattggaga aagacctttt
      241 tttaatgcaa agagtactaa taaagtaagt accaaaggaa ttaatcctgg aaatatctta
      301 tcaagtaaat ccgtttgaag actaaattta aaatcaccac tagtataact tattggtgta
      361 gataaattga taaacctacc tacaagcccc cccatgacta aggtacctag tatagaagcc
      421 ccttctataa ccttatttac tattccacct tgaagtatac tttcaacagc ttttttccca
      481 tacttatatc catttgtcca cattccaaaa gaaattgaag tcattattat atattgagct
      541 attacaaata atataggtcc tgcaattgat gctcctccct cagttaaacc aatacaaaca
      601 gctaacacta taggagttat aactccttga gttaaagtat ctccaattcc cgctagtgga
      661 cccatcaagc ctgttttgat agcattcata ccatctccac ttatttcatc tgctccatta
      721 gctttttctt cttccatagc tattgttatt ccatgtataa cacatccaca tataggttct
      781 gtgttaaaaa atgctagatg agtctccact tcttgtttta actcttcctt atctggatat
      841 aattttttaa gtactggcaa cattgaatgt gaaaaagctg ttgattgcaa tctttcataa
      901 ttgtaattag attgtgcaaa gaaaaaccat cttaaccatg attttattac atctttcttg
      961 cttaacgttt tcttagatac ttcatttttt atatctgaca taatattttc cccctcacat
     1021 gtatttaaga tgtagcacta tcttttccgt acttaattga agtaattgta agagcaagta
     1081 ttactccaat aacagatatt actattatat ctactttaaa ataaacagcc attagtattc
     1141 ctaatatgaa aaagggcata gtaaacttat ttccaatcaa ttttaaattc atagcaatcc
     1201 caagtgctgg caacatagcc ccaattacat taaatacatg taacacatta tcattcatta
     1261 cttccaacat accatctatc gcatttggac caaaataaac aactaataat gctggtataa
     1321 aactcattgc aaataacagt atctgtggtg gtactatatt tataaatgct actctatcaa
     1381 cattcccttt ttgaacctct ctatcagccc aatgtgcaaa aaaagaattt actgtcatct
     1441 ttccaatcca aagtacagtt gcaacagttc ccaatggtac agctattgcc attgcagtac
     1501 ttacatctag cttagatgct atagctaaag cagtacctac atatcctgca aaagctgggt
     1561 cccccatctg agctccccct gctgatataa aacctagata tatcatattt atacctgctc
     1621 ctataattgc tccttgaaca gggtctccta atataattcc cgttacaaat ccagccaata
     1681 gaggtctatt ccatgtaaaa aatccaacat aagtcaacca cggactccaa gaaagataat
     1741 aaaaaattgc tattaatact gcttgaatta gtgaaatgtg catattcttc ctccctattt
     1801 tcttttaaag atttacaaga tagtcttctt tacaataatg ttcctacatc aattttcttt
     1861 gcatctggta ctatttgtat aaagcattta acatcttttg atattagctc tctaaatatt
     1921 tctttttctt catctgacac agatatattt ttataaaaac tttttctacc aagccctgct
     1981 cccatacctc caacattcaa ttcctttaaa tctaccccac tatttactaa acctaagaca
     2041 gttttagggt atttgaccaa tactattaaa ttttcatctc cattatgtga ttttaagact
     2101 tcagcacctg attctacatc atgtacttca accttaacag ttgatggagc tgccatttgt
     2161 aaaacttgaa ctataaatgg gtcctttact actaaatcat ccacgattac tatccttttt
     2221 gctgatgtaa tcttacacca agcagtaata acttgcccat gtattaatct atcatcaatt
     2281 cttgttaatg ctatattact cataattatt cctccagatt tttaatttat tttacaactt
     2341 taaaacagtt ttaacatctt tgattcctga tatcccaact tcctcacata aattcttcaa
     2401 gtcttttaac ttcatagtat ctctgtttaa aaataactct aaaagcatag gtaaatttac
     2461 acctgtcaaa cattctaact cacatacctt agcaaattta tttattacaa tagcagatgt
     2521 attaaatgga gttccgccat aaaaatcaag caatactaaa accccttcat ccttatattt
     2581 ctcaattcca tcttctaact ttctactaaa ctcatctatg ctgtcaccat ggtttaatcc
     2641 taaagtttct acattttcct gtttgcctac tattaattca gcactttcta acattgcttt
     2701 agccatattt ccatgtgtgg ctataattat tcctgccata tgaatcatac ctttcttaac
     2761 cttttttata tggattatta tttagtactc taatctaatt atctatatta atcattatct
     2821 taccaaagaa aatatccctt ttgtatactt tttcaaaata agctggtact tcttctaaat
     2881 ttattctatg tgttactaat ttttcaacat tcatatcacc cttttgcata tgatacaatc
     2941 cagcactcca ttcttttcct gggaatggaa aggagtttcc aaaccaagtt ccctttacag
     3001 ttaattcact tcttactatc ttttcaaatt gctctctagt caaagctaca tccccatatg
     3061 gtacacctgc atacaatact gtacctccct ttttagctag caacagtact tgaccacatg
     3121 tcagtggagt accagccgat tcaataacaa tatctgcacc atctccatca gttaaccttt
     3181 ttatttcttc tacaatattc ttttcttttg catttataca aatatctgcc cctaactctt
     3241 ttgccaagtc taacttctca tcaaatacat ccacagctat tatttttgta gaaccaaata
     3301 tttttgccca ttgtattgaa aataatccta ttggacctgt accaagtact acaacagtat
     3361 ctccaacttt agcttctgac ctaaaaagac catgtcctgc tatacatact ggttctagcg
     3421 cagcggcagt ctcataactt atctcatctg gtatttttat taaatttctc tctttcacct
     3481 ttgtatactc tgcaaaacaa cctccaagtt ctttatttcc tattattgct acattattac
     3541 atcttgaata caagcctttt ttacattcat cacattcaaa acatggcata gctggacaaa
     3601 cagcaactct atctcctatt ttaaaactcc ttacttcttt accaacctgt gccacttctc
     3661 cagaaaattc atgccctaaa atttctccaa ccatatgtgg acctgtcttt gaatattttg
     3721 atatatcaga accacatatt ccagctactt ttactttaat tattacatca tctttttcta
     3781 atatttttgg tacatccaca tcctccactc ttgtgtctct tataccatag aatctaactg
     3841 acttcactat taaccacccc tactttttat ttgagaatat atccctattg tttatatgtt
     3901 aaatatctaa agcaactttc atgccaatat atgtaagcat tgtattcatg cattttataa
     3961 aattatcatt aaatgtgtgt caaagtgtgt caaaagtttc ttatattaaa acttatgtgt
     4021 gtcaatataa acgacactaa cattttatat tagttctctt attataagtt ttataaaatg
     4081 tacttttttt gttattttta taaaattgtt cggaaatttc ccttctattt gtgatataat
     4141 attaaatata aggtatcaat ataaattttc atgcgaatat cagaactttt ttgacactaa
     4201 tgccttattc aatataataa tttaactttt tggaggttgt ttatgaaaaa agtgtatcaa
     4261 ggaaagacta aagatgtttt tgaattagat aatggaaatt acttacttaa atttaaagac
     4321 gatgtaactg gtaaagatgg agtatttgac ccaggcgaaa atagtgtagg tctaagtata
     4381 gatggaattg gtagagctaa tttagagaca tctgttaagt tctttgaaat tctaaacaat
     4441 gctggaatta aaactcatta tgtaagtgct aatttagaag aagcaactat ggaagttcta
     4501 ccagcaaaag tttttggtaa tggattagaa gttatttgtc gttatagagc tgtaggtagt
     4561 ttcttacgtc gttatggttc atgtgttgaa gaaggtgcta aactagattg ttacgtagaa
     4621 gcaactttaa aagatgatga ccgttgtgac cctcttataa catctgaagg tttagaagct
     4681 ttaaacatta tgactcaagc tcaatttgat tcaatgaaaa gcatgactca aaaaatttct
     4741 aacatagttc gtgatactat agctgaaaaa ggtcttgagc tttatgatat taaatttgaa
     4801 tttggattct acaatgatga agtaatctta atagatgaaa tagcatctgg aaatatgcgt
     4861 gtttataaag atggtgtaat agttgaccct atggatttaa ctgctctttt attagataaa
     4921 tagtatatac atataattta caaatataat aaacttataa ataagataag ctatgaagta
     4981 aagacttttt aaatgttgat tatttttaaa cattttctcg cctttaattt catagcttat
     5041 ttaaattaat atactctaat tagtagataa tagctttaac cctacaattc ccaccacaat
     5101 aaaacctata catgcaattc ttgatatact aataggctca tttaaaatag ccatgcctac
     5161 aatagctgta ccgacggtac caattccagt ccatattgca tatgaagttc caagtggaag
     5221 atgttttagt gctaatgaca aaaaataaaa acttacaatc attccaacaa cagtaagtat
     5281 acttggagtt aatttagaaa atccatgtga attttttagt tgaaaagccc aaaacacttc
     5341 aaacaaacct gcaacaaaca aaaataacca tttcataaat tcatccctct tttcaaatat
     5401 gtctcttatt tttccaataa aataacctga gaaatattat aaaatatctc ccaggttttt
     5461 gtccttccgt gttcacaaca aaaactatgt tgtgggtttt atctcggacc agaccaatca
     5521 ttaataattg cggaacccta taaaactcta ttaattattc tattttatct ataataatac
     5581 tatcaacata tataaaaaaa gtcaatatat gggttccata tataataaaa ataactttta
     5641 tatacctttt aatattctta ttcacttctc tccataccca tttgggtcaa tctaagaata
     5701 tctaattttt atttaatatg ttataataac ataaaaatat tgaaaggagt aatttctatg
     5761 aatttctata aaattgcagt atgtcagatg attactacag aaaataaaat tgaaaatatc
     5821 aatcatgctg tagatatggt aacagaagct gccatcaatg gagctaaaat tgtagttttg
     5881 ccagaaatgt ttaactgccc atatgaaaat aaatattttc ctaaatttgc tgaagaatat
     5941 ccaggtgaaa caacaacgat attaagtaaa ttagctgaga aacatggaat ttatctagtt
     6001 tctggtagta ttcctgagtt agaagatgga aaaatctata atacttgcta tgtatttgat
     6061 aaaaatggag ctctaattgg aaaacatcgt aaaatgcact tatttgacat tgaagttaca
     6121 ggaaaagtta gttttaaaga atcagatact cttacagctg gaaatgatgt cactgtaata
     6181 gataccgaat atggaaaagt tggtatagct atttgctatg atattcgttt ccctgagtta
     6241 tcacgtttaa tggctcttaa aggtgctgaa atcgttatct tacctgctgc atttaatatg
     6301 actactggtc ccgcccattg ggagttatct ataagaatgc gtgctttaga taatcagata
     6361 ttctatgttg gtgctgcacc tgctagaaat atgaatgctt cttatatagc ttttggaaat
     6421 tcaagaattt cagacccttg gggaagaata attgctcaag ctgatgaaaa agaatgtatt
     6481 atttatgctg acattgatag agatttaatt cctgacataa gacaacagtt accacttctt
     6541 aagcacagaa gaactgattt atatgaactt aatactttga aataaaacag aacataaccc
     6601 cataaatcat aaattaagtg ttaaaaaaat ttatggggtt ctcattaatt tgatttaaaa
     6661 ttaatttcat aaaaataatg ctaatattaa tttaattaaa taatactaat aattactaat
     6721 gattatgcta attatcagca tttactaatt taatcactat tagttatttc ttttattaaa
     6781 tatctttact tttttactta ttatgtcttc tagctttaca tttggattat ggtatttctt
     6841 tctattttta gtcacatctt tctattgaat tacttgctta aaacaccaat gcacacatgc
     6901 cagataatgt tcacaattat ttagaaatct tgctctacct tcttcaatgt tgatattatc
     6961 tctcctagtt gatattatct ttcctaaaat ttcttttaat taaaaaacaa cattagttga
     7021 tatatcaagt atattttaag caactgaaaa aaacaataat aattgatata tcaactaatt
     7081 aatgtagatt aattcatttt tataatttat atacttttta attaatctac cttatttaat
     7141 taaaatcctt cctttctatt tatccatttt caacttaaat tataagattt catcagctat
     7201 atttttgcat accaataatt attaaatatc aaatatatat tctattaaaa tttctaataa
     7261 tttcatcctt gatttattta atatataaaa atatactaac tttaatgatt aagttttgaa
     7321 atataactaa gaatttgata att
//
LOCUS       DAELTA010000173         7325 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.174, whole genome shotgun sequence.
ACCESSION   DAELTA010000173 DAELTA010000000
VERSION     DAELTA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.174"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..942)
                     /locus_tag="KPG13_003139"
     CDS             complement(<1..942)
                     /locus_tag="KPG13_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG0986831.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKI"
     gene            complement(911..1357)
                     /gene="lspA"
                     /locus_tag="KPG13_003140"
     CDS             complement(911..1357)
                     /gene="lspA"
                     /locus_tag="KPG13_003140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG0986832.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1667..2446)
                     /locus_tag="KPG13_003141"
     CDS             complement(1667..2446)
                     /locus_tag="KPG13_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0986833.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2501..2983)
                     /locus_tag="KPG13_003142"
     CDS             complement(2501..2983)
                     /locus_tag="KPG13_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG0986834.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3249..4670)
                     /locus_tag="KPG13_003143"
     CDS             complement(3249..4670)
                     /locus_tag="KPG13_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG0986835.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(4816..5586)
                     /locus_tag="KPG13_003144"
                     /pseudo
     CDS             complement(4816..5586)
                     /locus_tag="KPG13_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5591..6793)
                     /locus_tag="KPG13_003145"
     CDS             complement(5591..6793)
                     /locus_tag="KPG13_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0986836.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         2437 a         1280 c          711 g         2897 t
ORIGIN      
        1 tatcttttta ataacttcct taaaatactc aggtaattct gaattaaatt cgatatattc
       61 cttagtagta ggatgtataa atcccaactt cttagcgtgc aaagtttgtc cttttaattt
      121 aaatttagtt tcttttggac catatacata atcacctaaa agtgggtgat ttatagacaa
      181 agcatgaact cttatttgat gagttctacc agtttcaagc ctagctctca tatgtgtaaa
      241 cttatcaaat ctttctacaa cttcaaagtg agtgattgca ttttttccat cttttactac
      301 agccattttt aatctatcct taggatttct ccctataggt ttatcaacag ttattttatc
      361 ttctttaacc acaccatagc atataaattc atactcccta gttatagtat gttcctttaa
      421 ctgttctgct agaaaattat gtgcataatt gttcttagct atcataagaa gaccagaagt
      481 atctttgtct attctatgaa ctataccagg tctaataact ccatttattg atgacaactt
      541 atctttacaa tgatataaaa tcccatttac taacgtattt tcatagtttc caggagcagg
      601 atggacaacc atatcctgag gtttatttat tattaagaca tcatcatctt catatacaat
      661 atctattgga atatcttgag gtaaaacttc taatacttta ggttctggta tctctataac
      721 aatattatct tcctctttaa ccagatatct agctttttct actttattat taacctttac
      781 ttttccatct tttataattt tttgtatata acttcttgac atatcaccta attgttctgc
      841 tagataaata tcaagtctaa cttcttcttc ctcttcaagt actaaaaact cttttacttc
      901 atccattata tcacctactt ttactttcaa aaaataaaac atatatacat aaaaacacag
      961 ttcctacaac tacaaataca tcagctacat tgaatacata ttcccatata actctaaagt
     1021 cgaagtaatc taccacaaaa cctaatcgta ctctatcaac tagatttccc aatgcaccag
     1081 atataattaa tataattccc aaccttccaa atatatgtac tttccttgta ttaagatagt
     1141 ataatccaaa taccgttgca agtaatgcaa caattataaa tatccattga ttattttgta
     1201 ataatccaaa tgctgcacct ctattttcga cataagttaa atgaaataca ttatttatta
     1261 ttggtattgt tgatacatcc accaaattat tcaataccca tattttagac agttggtcta
     1321 aacctatgag tagaattatt attaatatat atagcaatga tttttccccc tataaattat
     1381 ttaaaaacca attttatttg aagcacacta ttattattat acaaaaaaaa tgctctaaag
     1441 agcaacttta ttaaaattgt attttcttaa taaagctaga acaaaattta aaatagtact
     1501 acttttttta tattgtagat ttctacatat ttaaaatcat atcattatgt ataaaaaatt
     1561 taaaaatacc atactaatca atattttttg tgtattttat agattaaaga ttaatgatgt
     1621 ctgtaattta aacttacaat caccactaat cttcaaacaa atatttttac ttttgcactt
     1681 gcttattttt atctaaatgt tgtataagtt cagatactgt tacgaattta tatcctctct
     1741 cttttaattt aggaattact acttctaaag cttgtatagt ttgtgattta tcatttcttt
     1801 tagtgttata atcatgtaaa agtattatat taccattttg gacctttgta aggatagtat
     1861 ttataattgc atctactcca gggttagacc aatctctagg gtctaagttt gaccaaagaa
     1921 ttatattcat tcctttagaa actataatat cacagacact tttactcatg gctctatatg
     1981 gaggtctaaa tatctttggt tctactccta tcacactttt tataatattt tgcgtatctg
     2041 ttatttcttt ttcaacacta ctataactat tttttgcaac atttatatgt gtaaatgtat
     2101 gattccctat ttcatgcccc tcatcatatt gtctttttaa taattctttg ttatattcta
     2161 ctttttctcc aactacaaaa aaagttgctt ttacatcatt tttctttaat atatcaagta
     2221 cttgtggtgt aaaatcctca tctggaccat catcaaaagt caatgctaaa attttttcat
     2281 cctcatttcc tttttttatt attacatctt cataatcgtc ttttatattg tcatttaaac
     2341 tatatgcttc ttttgaacta tcttgattat gtgtattatg attatatgta tatacaccta
     2401 ctatagataa taaacatata catataccac atattatttt cttcatgtca ttacctcttt
     2461 tctaattcat tctagcaata tattttataa tgtcagtttt ttaatctctc aatattccat
     2521 ttttatttaa atcagttaaa tttgttaggt cttctgaata aaagttagaa tcttctacat
     2581 cagggttttg cattgcatca cctaataaat tattttcctt ctttgcacaa tcattacata
     2641 aacttgtatc tggcaatata tccaatcttt ctaaatctat ttgattatga cataaatcac
     2701 aaatcccata tgttccattc tctattttag ataatgctaa gtctatttga tacaatcttc
     2761 cctcttcatg atttattaga ctgttttgca tattttgcat atataaatct gttcccatgt
     2821 ctcctatatg attatcataa ctagataatt ctcctgatga atacttttca cttgtatgtt
     2881 tagttgtatc tccaaataaa gtattatctt tcatatcctc tactaaatta gttaaatttt
     2941 ctctttcttt caataattta tctttatatt ttttagtatc catttttaac acctctaatt
     3001 ttaatattta catcataaaa atagtatgtg gaaaagtaaa ttaaacatac aataaaaata
     3061 agatttgatt cgaaaaatgg aatgtctcaa aatgaaattt ttagttcatt taaccctctt
     3121 ttttatatga aatcaaatat atttttatcc tttcagcaat aaaaaagaga cttatctcta
     3181 ttttgagata gtctcttctt tgaacataat ttaaatacaa ttttttaatt tttatagtta
     3241 ttattttact aacttgccaa tttatgatta ttttgttctt taatatgttt tgctttttta
     3301 aagaataaaa gaccaagaat tattgctaca ataacaccta ttccattaga tatattaact
     3361 ggtagtctaa atccttcttt tgcttgcatt atataactaa atgttactac agtcataaaa
     3421 agtgctggta atgtagttat ataatggttt ttatttgctt tgactaaata tgcagaacca
     3481 gtccacaaca ttatcattgc aagtgtttga tttgcccaag agaaatatct ccaaattata
     3541 ttaaaatcta taaatgtaag agcaacacca actgcgaata atggtaaagc tattaaaaat
     3601 ctatttttga ctgcatcttg attataattc attgcatctg ctattgtcag cctagcactt
     3661 ctaaatgctg tatctccaga agttatagga catgccacaa ctccaagtat agctaaagct
     3721 cctccaatag gccccattaa tccaacagat actgtattta ctactgttgc tgctccacca
     3781 tcattaaata tcacattaag ttgaggtatg ccatggaaat atgacatggc tgctgctgcc
     3841 caaactaaag ctattatacc ttctgcaacc attgacccat aaaatactgg tcttgcctca
     3901 ctctctttct caacacatct cgccataagt ggtgattgag ttgcatgaaa acctgatatt
     3961 gctccacatg ctattgttat aaataagtat gggaataaag gtgttccatt tggattcata
     4021 ttttgtaaat ttatatttgg tatgttatac ccctttatta ttaatcctcc agctacacct
     4081 actgccataa ttaataatgc tgctccaaat aaaggatata ttttaccaat caatttatct
     4141 attggtaaaa cagtagccat aatataataa agtattatca ctccaaccca tatttgtgcg
     4201 tttacacctg tcaatgtctt taataatcct gctggacctg ttacaaatac aactccaacc
     4261 aatactaaaa gaataacact aaatattctc attgctattt ttgcaccatt tcctagataa
     4321 aggcctacca actctgatgc agatgtacct ttgtttttca ttgatagata tcctgaagca
     4381 aaatcgtgta ctccaccagc aaagattgtt ccaaacacaa tccataaaaa tgctgatggt
     4441 ccaaataatg ctccttgtat agctccaaat atcggaccta accctgctat atttagaaac
     4501 tgaattaaaa agattctctt ccatttcatt ggcacataat ctactccatc cctacaagct
     4561 atagctggag tttgattttt gtcattggtt ccaaaaacct tttctacaaa agtaccatag
     4621 acaaaataac ctactataag tactactatt gaacctaaaa agcttaccat aaattcatcc
     4681 ccttagtttt tatataattg ctttataaat ttttatgagt ttaagaaaac tatttccagt
     4741 tcaaatagta atactttttt atgctaatat aaatattaat tacattaagt gcatatttaa
     4801 gtacatgaaa tgactttata tatgcattaa cagcctaaat tcttttactt tgcttctact
     4861 tacagttact tcataatcac tattttttaa ttttaatatg tatgtattat taaaccatgg
     4921 tataatttct tttactttgg ttaagtttac gatataagac ctatgtgttc taaaaaatgt
     4981 tttatcattt ataagatttt cgacttctta tatgccttct tttaatataa acttttcttt
     5041 ctcagtatat ataaatgtct gcctttcatt agcttcacag taatatatat catcaatatc
     5101 tattacaact aatttatcac ctttccaaag gctaatttta tgtgttattt cttctatctc
     5161 ctgatttact gcttccttct tatatttgta taaattacta tttacattaa atagttcaat
     5221 atcactcatc tcactttttt ctaatttatt cagcatagaa ataattctct cttcagaata
     5281 tggtttcaat atataatcaa atacatccaa agaaaaagca tctactgcat aactttcata
     5341 tgctgttata aaaacaattt taggtcttga tttaaactga tttaatgttt ttgcaagtag
     5401 cattccatca agatgaggta tatttatatc taaaaaaatt acatctattt tattttcctg
     5461 aataaagtct aacacctcta taccattggt aaattcactt actacctcga ttccactttt
     5521 attttcaata aaatatctca attcctttct tgctggaaat tcatcttcca ctattatagc
     5581 tctcatactt ctactccttt acataaaata ctatcaaagt cccattatct attcttctta
     5641 tattaagccc tcttccatac atcagtttaa gccttaaatg tacattataa agcccaatct
     5701 tattttctgg catattatct tgatatacat tatttataat ttcctcactt atacctatac
     5761 cattatcctc tattgatatt ctcacagtat tttctaatga atacggtaac ttttttatgg
     5821 atattttcac tacaccagct ctaccacttt ctaaaatacc atgtattatt gcattttcca
     5881 ctaatggttg tataatcaaa cttggaatct ttatatttac atcttctata tcataagata
     5941 cagttagtaa ttctccaaac ctagcttttt ctatctctac aaaagattta acttgttcaa
     6001 tctctttatt tatatcaatt aagttatcac tatattcaag attatatctt agataagttg
     6061 ataaatttat tattaactct ctagccttat ctggactaaa tcttataaat gaagctattg
     6121 tgtttaggga attaaacaaa aaatgtggat taatttgtct ttgaagtgct tttatttctg
     6181 ctttatttct cgcttctata tcttttttat ctttctcaat cccctctact atagatataa
     6241 caaatcctat tccaatctgc ccaactatta aaggtaaata tattccaccg attatttgaa
     6301 ttgctaaaga atgtggatat gatattaagt atataagtaa tatagtaaaa ctttcagaaa
     6361 tcatgcctac caatattcca tacattactc tatgttgttt tggtatcctc ttatatagaa
     6421 atcctgataa tacacctgct agtatacttg ataaaagaca tggaacagat gttatccccc
     6481 caatatccat aaaatatcta tgaatccctg aaaaaactcc tgctgtaata cctaccatcg
     6541 gaccaaataa aatgccacct gatactatag atattattct agtgtttact attgacccca
     6601 tataatttat accactataa gttccaaata tggctaaaaa agtaaaaact aatgctatac
     6661 agaccaagtc attaaaacta tattcttcct tttgaaatat ctgcttaaat atttttaact
     6721 ttgttattag aagaaacaat actacaataa ggcttgtctt ttctattaat gcgattaaaa
     6781 ttgatgtttt cataatcccc ctttaaaaaa taaattttta cacactctaa atttattatg
     6841 gttgttataa attacatatt tgaagtaatc ctatcacaaa actttccaca tttaatcttt
     6901 taattaaata agaaatagtt gtacttctat taacaactaa tactagctag cataagcttg
     6961 taataacttg tagttattat taactttaca attctagtat ttaattttat actttagaaa
     7021 tctcttttct tagaaataat actttcgaat atcatataat acaatattat gtagattctt
     7081 actcatacaa catatttctt taaaaatttt atactattct ttgttcaaat atctcatttg
     7141 atttaattgt gttaaacaca cattatcatc tctctgttat atatttatta tatagccgta
     7201 tatcgataaa gtaaataaat cccatattta aaataatatg tttgacaata aattattata
     7261 atgtatattt accataataa tttgtcaacc ttgaaagtta cttctataat tattgttaaa
     7321 taatt
//
LOCUS       DAELTA010000174         7320 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.175, whole genome shotgun sequence.
ACCESSION   DAELTA010000174 DAELTA010000000
VERSION     DAELTA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.175"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..839
                     /locus_tag="KPG13_003146"
     CDS             84..839
                     /locus_tag="KPG13_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0986837.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            970..1992
                     /locus_tag="KPG13_003147"
     CDS             970..1992
                     /locus_tag="KPG13_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0986838.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2156..2713)
                     /locus_tag="KPG13_003148"
     CDS             complement(2156..2713)
                     /locus_tag="KPG13_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0986839.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2719..3795)
                     /locus_tag="KPG13_003149"
     CDS             complement(2719..3795)
                     /locus_tag="KPG13_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG0986840.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3796..4479)
                     /gene="modB"
                     /locus_tag="KPG13_003150"
     CDS             complement(3796..4479)
                     /gene="modB"
                     /locus_tag="KPG13_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG0986841.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4628..5440)
                     /gene="modA"
                     /locus_tag="KPG13_003151"
     CDS             complement(4628..5440)
                     /gene="modA"
                     /locus_tag="KPG13_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0986842.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5633..6562
                     /locus_tag="KPG13_003152"
     CDS             5633..6562
                     /locus_tag="KPG13_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986843.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6723..7172)
                     /locus_tag="KPG13_003153"
     CDS             complement(6723..7172)
                     /locus_tag="KPG13_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986844.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
BASE COUNT         2550 a         1189 c          925 g         2656 t
ORIGIN      
        1 tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta ccaaaaataa
       61 atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag agtttcccta
      121 atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata gaagatggtg
      181 aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta aatataatat
      241 ccgggcttat aaaagaatct tctggtcaag tattattaaa taaacataac ttgtctaatt
      301 tagctgaaca cagaagaact caaatagtaa gtagagtttt tcaagaccca agtcttggaa
      361 cttgcccttc tatgactgtt agagaaaact tgtctttagc tcttaacaaa ggaaaactat
      421 taaatctaag aaaatgtctt cgttacaaaa gagatttctt agaaaattta ttggaaggtg
      481 tatctcttga tttaaaaaaa taccttgatg ttcaagtaaa atttttatca ggaggtcaaa
      541 gacaatcatt atcactaatt atgtcgtgct taacaaatcc atcagtttta ttacttgatg
      601 aacatacagc agcacttgac ccaaaaacat caaatgaagt aattgaattg actaataaga
      661 ttgtaaggga aaaaaatata actacactta tggttactca caacttaaaa cacgctcttc
      721 attatggtga tagattgata atgcttcata agggtgaagt tgtattagat gtaaaaggta
      781 aagaaaaaga acaattaaca gttgaagaaa tcttagaaaa atttgaatat gcagtttaaa
      841 ataatatatc tattgtatta aactcggctt taaaataaag ttaaaattac aataattatg
      901 gttactttgt aataattcat atgaatatat gaacatatat aacaataaat ttttgagggg
      961 gatataaaaa tgattaataa aaaaagattg gcaagtttga ttttagctgg tgctcttagt
     1021 atatctatgc ttacagggtg ttcacaagga ggagactctg gtaattcaaa acaagaaagt
     1081 aattctaaag gtaaagaagt taaaaaaatt ggtataactc aactggttga acatccagca
     1141 ttagatgcca ctaggacagg ttttgtaaaa gcattagaaa agaacggatt taaagatgga
     1201 gaaaatattg atatagactt ccaaaatgct caaaatgaca tgcctactac acaaagtatt
     1261 gcgagtaaat ttgcatctga taagaaggac ttaatatttg ctatatctac tccatcagct
     1321 caggcagcat ttaatgctac taaagatata cctatactta ttactgctgt gagtgaccct
     1381 gttgctgctg gattagttaa aactcttgaa aagccaggca caaatgtatc tggaacatca
     1441 gattttgttt ctgtagacaa aggtttagaa cttcttaaaa tatttgctcc taaggcaaaa
     1501 actataggag ttatgtacaa tactagtgaa gtaaattcca aagttcaagt tgatgcttta
     1561 aaagaatatg catctaaaaa tggatttaaa gttgttgaaa aaggtataac tacttcaaat
     1621 gaagttaacc aaggtatttc tagtttagtt ggcaaaatag acgtattata tgtccctaca
     1681 gataacttag tggcttcttc tatgcctata gtctctaaaa tagctactga gaataaaata
     1741 cctgttatag cagctgaatc tggtccagtt gaaaaaggtg ctcttgcttg tcaaggtatc
     1801 aactacgaaa aactaggtta caaaacaggt gaaatggcag ttaaaatttt aaatggcgaa
     1861 tcagtatctg atatgcctgt ggctacatca gatgatacag atatcattgt taatgaagat
     1921 atattaaaag ctttaggtat ggaaaaacct tctaatgaaa atatttctta tgtaaagact
     1981 aaacaagaat agaaagatat tttctatata cgattaatat gcctgtttct ataaattaat
     2041 ttatagaaac aggcatatat ttttatttta atgtattatc tatttaaaga ttatatgtaa
     2101 atgctcttaa tttgtttgta taaattttac ttgtataaac tttatacaat catatttatt
     2161 cttctatttt ctttattact ctacatggat ttccaacagc tacagtattt ggtggtatat
     2221 cttttgtgac tacacttcct gcacctatta ctacattatc acctatagta attcctggtg
     2281 ttatgattac tcccccacct atccatacat tatcacctat ttttatagga gaaccatact
     2341 ctatgcctga atttctaagt tgtgcatcaa ttggatgata ggctgtatag atttgaacgt
     2401 ttggagctaa cattacattg tcacctattt caattttaca tacatctaaa aaaatacaat
     2461 cataatttgc aaagaaattt tcgcctacat gtatgttata tccataatca cacctaatat
     2521 tttgttctac atttatttgc ttgccaactg aaccaaatag ttgtcttaaa atatcttctc
     2581 ttttttcata ttcatcctct aaagtattat taaacaatct agttaatttt ttacaatact
     2641 ctctttcttt tactaacaat tcatcatttg catagtatcc tttcccagac aacatttttt
     2701 ctttttcagt catatagttt actccttttt gttataattg tatctaaagc aaaataaata
     2761 ttcctgtgga aaatctaaat atattctgtc acctttctta aatctcattt tacttttttc
     2821 caattctatt tgaataggta cacattcgtt agataaatca gtttttctaa catatatagt
     2881 atatgtaaaa ggattttcta caatgttaat caattcgaaa cataactttt catttaaatc
     2941 ttcattagaa tccaagacct taatatgatg ctccctgatt cccacatatt ctatattatc
     3001 acctatttct ctatttaatg tcaactcgca tccccagtct gtagcataaa tagtattttt
     3061 atccagtcta tttaatttgg atatattttt acaccctgtt atcttagctt ctataaggta
     3121 ctttggagat tcaaagagct cttttttagg tctctttgga agaccttctc ctttgttgta
     3181 cactataata tcatcacaaa ctcggtaagc ttcttcaata tcatgagtaa caaatagtat
     3241 atctcccttg taatctttta acatattcat aagttctttt tccatattgc ttcttaaatg
     3301 atggtccaat gctgagaaag gttcatctaa aagtaatata tctggagatg ttgccaatgc
     3361 tctagctagt gctacccttt gttgttgacc accagataat tgccaagggt atctcccctc
     3421 aaacccattt aatttaagta tatctaagta ccttgctact atttcctttt tttcactctt
     3481 acttagtttt aaaagcccaa gttctatatt ttgacttaca gtcatatgtg gaaacaaagc
     3541 atagttttga aataaaaatc ctacctttct atcttgtgtt gataaattta tcttcttttc
     3601 agaatcaaat agaactctat catttaatac aattttacct cttgttggtt tctcaagccc
     3661 tgctatacat tttaatgtca tactttttcc agaaccagat tctcccaaaa accccagagt
     3721 acctttttct tgtttaattt ctacctttag tttaaaagag gataaatctt tttcaatatc
     3781 cacgtataag ctcaactatc cacacctctt tcctataatt ttctgctgaa attccgacca
     3841 ataatttagt aataaaatca ttgctataga tattgctaca ataatcataa cccacatcat
     3901 agctttattc atatctccgc cttcaacagc aaaaaatata gctattggca tagtttgagt
     3961 ttttccaggt atatttccag ctatcataag tgtagcacca aattctccaa gagctctggc
     4021 aaacgacagc accaatccac caattattcc aggccatgct aatggaactg caatcttaaa
     4081 aaatactttc cattcattaa gacctaatgt tctagcagct gacaacatat tattatcaat
     4141 ctgttcaaaa gctgaccttg aagttctata catcatagga aaagacacca ctactgctga
     4201 aattacagtt gctgtccaac taaatattaa agtcctatcg aaatttaata aaaactttcc
     4261 tataaatcca ttcttcccac atagtaataa taaaaagaaa cctacaactg ttggtggcaa
     4321 aattagtgga agtgtaaata gtccatctat aagacccttc cacttacctc tatagttagc
     4381 cataatatat gacacaaata ttcctattac aaaagttatt attgttgaca aaatagatgt
     4441 cttaagtgat atccataagg gtgaccaatc tgttcccatc tttccctcct taattttaaa
     4501 tggaaatagt gaaactaata actctattaa tttcactatt tccattttat tataatacac
     4561 aattattttc aattttcatt tatttatata taatttatac ttaattacac ataatttatt
     4621 tctaagttta ttctactttt ttatatccaa attcttcaaa aatcttttgt cctgactcac
     4681 ttaataaaaa ttcttcaaat ttcttagcag catctacgtt tttactagct ttaattactg
     4741 aaactggata tgtaataggc gaatgagttt tttcgtctgt tttttcaact acttttatct
     4801 tatcattatt gacagtatca ctgaagtaaa cgaatccaac atcagcattt cctgattgca
     4861 cccatgctaa aacttctttt acatcttttg caaatactag cttatctttt aacttatctt
     4921 ttaaatttaa attagttaac acctcatctg catacttacc agctggaaca ctttcagctt
     4981 caccaacagc aattttcttc actttatctg tagtcaaatc tttcatacct gatacactac
     5041 tgtcagcaga agaaataaga actaaatcat tttttactaa atctttcata gtatcagaaa
     5101 ctaataactt ctcttcatcc aaagctttca tttgtttttg acctgctgaa ataaataaat
     5161 cacaaggtgc tccttgttct atttgttgtt gtaatgagcc tgatgctcca tagttaactg
     5221 taagttttac atttgaatca acttttttat attcttcttc aatctttgcc attgcttctt
     5281 ttaaacttgc agcagccgat atatttaact ctactgaatc actactttta gttgtttttt
     5341 cttgttttcc atcatctttt ttgtcttcat tactattgca cccaactgtc cccaaagtta
     5401 aacatgcaac taatcctaat attcccaata tttttttcat aacaatctcc ctttttgtaa
     5461 atttgataca cattttaaca tgttttatta tgttttatca tgttttgtta tatatagttt
     5521 aattatatat acatgataat aaattttcaa gtaatttatt tacactactt ttaaatattt
     5581 tgctacattt tccttttttt aatgttaaaa taaccttata tggaggtgga ttatggactc
     5641 attatcatct ttgacacctg tcgaggtagc tgaattatta aagataacaa aaaatactgt
     5701 atatgaatta atcaaaagag gtgaacttcc ttcttataaa gtagggaaaa aaataagagt
     5761 tgatactaaa gatgttgaag aatatatcaa cagccaaaaa actggtaaag taaaggatac
     5821 acatttcaac aataaaaata tagatataaa taataatttc aaaattatta tttctggtca
     5881 agatgtaata ttagacattt tagctaggtc tgttgaaaaa aaattggatg gaataagtac
     5941 atttcgctct tatataggta gttataatgc tttgtatgag ttatataatg gcagagtttc
     6001 tatagcatca tcccatctat gggatttcga aacggatgaa tataatagca cttttgttaa
     6061 aaagctattg ccaggaatac catgtgtact tataaatcta gcttatagaa tgcaaggttt
     6121 ctatgttgct aaaggaaatc ctaaagagat aacaacctgg gaggatttaa caaaaccaag
     6181 tataaccatt gtaaataggg aaaaaggttc tggaactagg gttttactag atggtaaact
     6241 tagacttcta aactttaatg gtaaacatat aaaaggttat gataatgaag aattatcaca
     6301 tcttggagtt gcaagtactg tttctcgtgg aatagcagat gttggtttgg gaaatgaaaa
     6361 agctgcttta caagtaaata atatcgactt tattcctctt cacaaagagc gctatgattt
     6421 agttattaaa aaagaagact tacaaaatcc agtttaccaa actataatta acataataaa
     6481 ctctcctgag tttaaagctg aacttcaagg tcttggcgga tatgatttaa ctgatactgg
     6541 aaaaactata gcaaaagttt aagattaaaa tcgcaatcta ttttataaga tttataattt
     6601 ttcacaaaat ttataactta aacacgaaat aaattgcgat tttagtgtct caaaaaattt
     6661 atgtctttca ttttatcatc caatacaaaa tataatataa ttaattgtgc aatcatatta
     6721 tattaatata tatttacata atagtatttt tctttaatgt agattaacat atgatatgtt
     6781 ttaaacatca atccccttgc tttcttatat atatttggca acacatatga gtttaataaa
     6841 ttatatacaa tgttgctttc ttcttgtatt ttttcaacca acaagttatt tataaaatta
     6901 cctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat atatcttccc
     6961 actaggtata cacactctac aataaataat aaaaatacaa tgcagtcagt acctgttgtc
     7021 ttatataaat tacccgatac taatggcttt gcgaagaaat tataaaaatt caaagtatca
     7081 cccgtataat caaaaagtat taatagcact cctagaactc tcaatacttt aatattcaac
     7141 atgtttttat ctttattatt tttgtttttc atatatacta cctctctttt ctttagattt
     7201 gattgtaatc taatactaac tcttttattt gaggtataaa tgaatttaat tggtagttat
     7261 tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt aaacataaaa
//
LOCUS       DAELTA010000175         7294 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.176, whole genome shotgun sequence.
ACCESSION   DAELTA010000175 DAELTA010000000
VERSION     DAELTA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.176"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            180..392
                     /locus_tag="KPG13_003154"
     CDS             180..392
                     /locus_tag="KPG13_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0986845.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            405..626
                     /locus_tag="KPG13_003155"
     CDS             405..626
                     /locus_tag="KPG13_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0986846.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            726..2882
                     /gene="feoB"
                     /locus_tag="KPG13_003156"
     CDS             726..2882
                     /gene="feoB"
                     /locus_tag="KPG13_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG0986847.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            2908..3060
                     /locus_tag="KPG13_003157"
     CDS             2908..3060
                     /locus_tag="KPG13_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG0986848.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            3240..3950
                     /locus_tag="KPG13_003158"
     CDS             3240..3950
                     /locus_tag="KPG13_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG0986849.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            4035..4805
                     /locus_tag="KPG13_003159"
     CDS             4035..4805
                     /locus_tag="KPG13_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986850.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            4798..5529
                     /locus_tag="KPG13_003160"
     CDS             4798..5529
                     /locus_tag="KPG13_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986851.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            5563..6522
                     /locus_tag="KPG13_003161"
     CDS             5563..6522
                     /locus_tag="KPG13_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0986852.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(6620..7216)
                     /locus_tag="KPG13_003162"
     CDS             complement(6620..7216)
                     /locus_tag="KPG13_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG0986853.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         2734 a          794 c         1368 g         2398 t
ORIGIN      
        1 tacaataatc acttgcaaaa aatatactat tgtgatagac ttaaatcaaa attaggaatt
       61 gtaaaaagaa ggaaaaaata ggatgttatt ttaatagatt aagatttaag gtattgtaat
      121 ttatatataa gataacgatt gcttgtagat ttaaattact ttaagaggag ggatattata
      181 tgatgccatt gatattatca aatacaggtg aagaagtagt tataaagaaa atttcgggaa
      241 gtgataagac gaggactttt ttaaatagta taggttttgt agtgggtaca aatgttaaaa
      301 tagtttctaa gatagatggt aacttaataa tagacgtaaa agatactaga gttgcattag
      361 ataaaaaaat ggcaagtaga ataataatat agggaggtaa tgacatgaac agactgaagg
      421 atattaagtg tggagagact gtaaaagtca aaaagttaga gggagaagga gctacacgaa
      481 gacgtataat ggatatgggg attacaagag gtgttaatat atttataaga aaagtagctc
      541 ctcttggtga cccaattgag gtaacagtca gagattatga actatcaatt cgtaagtcag
      601 atgcagaaaa aattattgtg gaataataaa gattaattag agtagggtta atgtaattat
      661 ataatgagaa tgattttctg tatcacaaac agtgatgtat ttaaataata atggagggaa
      721 tggaaatgtc aataaaaata ggtctcatag gaaatcctaa ctgtggtaag acaacaatgt
      781 tcaatgggct tacaggctca tcacaatatg ttggtaactg gccaggagta acagtcgaaa
      841 aaaaaggtgg aaaattaaaa ggaaataaag atgtggaaat agtagattta ccaggtattt
      901 attctttatc tccatataca ttagaagaag tagttacacg taattttatg ctagatgata
      961 agccggatgc agtaataaat attgtagatg catcaaatat agagagaaat ttatatctta
     1021 caactcaagt tttagagcta ggtataccga ccgttatagc tcttaatatg atggatattg
     1081 taaataagaa tggtgataag ataaatatta aagaactatc tgaagttata ggttgcccag
     1141 tggtagaagt aacagcagtt aaaggtcaag gaattatgga ggctgctgaa aaggcagttg
     1201 aacttgcaag tagtaacaat aaattaaatt tcaaattacc ctttgttgat gaaagtaagg
     1261 atgcaataga aaaaattgaa aaaataatag aagaaaagac tccatatata gatgtagaaa
     1321 cacgctggtt agctataaag ctatttgaga gagatgaaaa tgttatacaa aaacttgata
     1381 tttctaaaac aatattgaat agtattgaag aaataactag aaattgtgaa gatgaattgg
     1441 atgatgatag tgaaagtata ataacagcca atcgttatga atttattagt tcaataattt
     1501 caagtataat aaagaagaat cgaaaaggta aggaaactgt atcagataag atagataaga
     1561 ttgtaacaaa tcgtatacta gcattaccta tttttgcact tataatgtgg ggagtttact
     1621 atatagcagt aagttcattg ggtactatag caactgattg gacaaatgat gtactgtttg
     1681 gagaaattat tcaaggcaat gtaagtaatt ttctagcttc tttaaatgta gcagagtggt
     1741 tacaaggact tgtagttgat ggtctaattg gtggagttgg agcagtacta ggatttgtac
     1801 cacaaatcat gcttttattc ttattattat caatattaga agattgtggg tacatgtcac
     1861 gtgtagcttt tataatggat agaattttcc gtaagtttgg tctttcagga aaatcattta
     1921 taccaatgct tataagttca ggttgtggag ttccaggagt tatgtcaact cgtactatag
     1981 agaatgatag agatagaaaa atgactatta tgttaactac attcattcca tgtggagcta
     2041 agattccaat aatagcatta tttgcagggg cattatttgg aggtgcttca tgggtagctc
     2101 catcaatgta tttcttaggt attgcaatga taatcatttg tggaattata ttaaagaaaa
     2161 ctagtttatt tgcaggagaa ccatctccat ttgttatgga attacctcaa tatcatattc
     2221 caagtgcaaa aggtgtttta atacatatgt gggatagagg aaaagcattt ataatcaaag
     2281 caggaacaat tatatttgtt gcttgtggag taatctggtt cttacaatca tttaattggt
     2341 cattacaaat ggtagatgct ggagatagta tattagctag cttaggaaat atagttgcac
     2401 caatatttgc tcctcttgga tttggaaatt ggcaatcatc agtagcaaca gttacaggtc
     2461 tagtagctaa ggaaaatgta gttggaacat ttggagtttt atttggtata tcagatgcaa
     2521 cagagcaaga cccaacactt cttgcttcag ttgcaagtat gtttacagta gctagtgcgt
     2581 ttgcatttat ggcatttaat atgctttgtg caccttgttt tgcagctatt ggagccataa
     2641 aaagagaaat gggttcatgg aaatggacat ggattacatt aggattccaa actttaacag
     2701 cgtatataat tgctcttctt ataaatcaag ttggaagttt agttttaggt actggaggaa
     2761 gtattgctgg ggcaataata tctatattta tagcagtagc agtagtcttt gtagttttaa
     2821 cttattcaaa taaaaatatg aaaaaagaaa aaatgggtaa attaagttat atgaaaaatt
     2881 aattcatata aataaggagt gatgaatatg gctacattta ttatagcagc tattgtagta
     2941 gtattaatgg cactagctgt aatgtatatg gttaaaaatt caaaaaagaa tggtagcagt
     3001 tgtggatgtg gttgttctgg atgctcaagt agtaagagtt gtcatagcgc taagagataa
     3061 ataggataag agcctgataa aaagttaaat tgctttatta tcaggttctt attttttgta
     3121 taaataacaa atttcatatt tgaagtaggt tagactatgc tgtaatttta ctgtataatt
     3181 atatataaat atatgattag gagggattat tgctaaatta agcaatataa gtgaatatta
     3241 tgtttaaatt tgatgttaca aacgagctta tgtgtatagc aaggggaagt ggtaaatttt
     3301 ttgcaaaaaa aggtgctatg gtagccttta aaggaaattt taattttgaa aaattgctgt
     3361 taggtccaag taatggcggt ggattgggaa gagcattatt aggtcatgtt cagaggtcat
     3421 tgacaggaga acaaatgcca ataatggtgg ttgaaggtga aggtgaaatt tacctagctc
     3481 aaaatgctta ccatgtagat gtaatatcaa ttgacccagg tgattccatt tatgttgaga
     3541 gtgaaaactt attggcattt tctgaacaat taaattacag tgtaaaattg attggttcag
     3601 gggtaatatc tcaaaaaggt ctttttacta cacatttagt aaataaaaca ggtcaaaatc
     3661 aagatgttgc tataataaca gatggtaatc ctctaattct tgaaggacct tgttgtgttg
     3721 acccagatgc attagtagct tggactggaa gagaaccata tcctaaagta tcaaaactat
     3781 catggaagac ttttattggt cagacttcag gagaatctta tcatatggaa tttgttgaac
     3841 ctggacaaat agtcattata caaccatctg aaagattgtc tggcttaaaa acagattcta
     3901 caccttctga tagaatgagt tcaggtggat taagacttgg catagattaa atcttatgat
     3961 atagcttaat tattatttaa gatattaaaa aataaaaagg gtagcaggga tagatatgtt
     4021 aaaaggagtt ttatatgttt gagaaaattc ataaaaggct taaaggatat tatatttttt
     4081 taataatttt aattttatgg aagatagtat gtgttttggg tatatggagt agctatattc
     4141 tgcctcctcc tgaaatagtc ttagatactt ttataaaaat gatatatgac ggttctattt
     4201 tcttaaatgt ttatgccagt gtaagaagga tattaatagg ttttttaata agttcattga
     4261 tggctatacc acttggagtt ttatttggag caaataaaaa aatgtatgaa tatttcaaac
     4321 cactatttga atttatgcga agtacacctc cactagcttt ggtgccaatg cttattttat
     4381 ggtttggtat aggtgaagaa tctaaaatta taattattat tttggcatcc ttttttccaa
     4441 tatttttaaa tacattgaaa ggtataaaaa attgtgacaa taaattaata gaagtaggta
     4501 aatcttttaa tttatctaaa aagagaattt tttataaaat aataatccct aattctatgc
     4561 tggatattgt tgttggactt agtttagggc ttgggtatag ttttagggct ataattggag
     4621 cagagcttat tgcagcttct tcaggactag gatatttgat ttctgatgga aaagatatgt
     4681 ctagaactga tgttgtattg gtaggaattt ttgttattgg atttttaggt atactttcag
     4741 attatctatt ttcaaagata ataacaaaat ttagcaaagg gaagcaggtt gatttaaatg
     4801 gttagaagtg gatttttgct agagaatata tataagaaat atttagttga taataaagag
     4861 catttagttc ttgataatat atctttaaat atatctagtg aagagataac tgttatatta
     4921 ggtgaaagtg gatgtgggaa aacaacgcta cttagaattt tagcagggct tgaaaatgct
     4981 actagtggaa acgtatattt ttttaataat gataaaaagt gtactccaaa agtaggaatg
     5041 gtatttcaag aaagtagact tatgccatgg ctgaatgtaa gtgaaaatat acttttacat
     5101 actgaaaaag ataatagaaa taaagttgat ttagacaagt atttaaaaat gatgaagctt
     5161 gaaaagttta aaaactcata tccaaatgaa ttatcaggag gaatggctca cagagtaagt
     5221 atagcaagag cactttcctt caatcccgat attttgctga tggacgaacc atttgcagca
     5281 ctagattact tcacaagaag aaaaatgcaa aaagaagttg taaatatcca taaaaatact
     5341 aaaaaaggtg tagtatttgt aactcacaat attgaagaag ctatggaaat agcaaagaaa
     5401 ataattgttt ttagtaaaaa taaaaggatt aaacaattta gtgtcgaaga tgaatataat
     5461 agagatttaa ctaaaaacta ttatataaac ttgaaaaaag aaatattaag ggaattgggg
     5521 gagttttaat tgattaaaag aaacaaaaag attttagcag taatgacatc tttaataatg
     5581 gtgttggttg ggactgtagg atgttcaaca acaaatgata agaaagatga acctgcaaaa
     5641 aaagaggcta gtttacctaa ggaaataaat ttaacttatg taaaatcacc acttaatgtt
     5701 cctagtatat tagaaaaaaa ggatgattta tttggaaagg aatttaaaaa ggacaatata
     5761 aaagtcaatt tccatgaatt gacaacagga ccagagcaaa ctcaagcatt agcagcaggt
     5821 gagctagact ttttacatgc attaggagga acgtctgcaa taatagctgc atcaaatggt
     5881 gttggtctta aaattacgaa tatttatagc cgttcaccaa aaggatatat gcttataact
     5941 aataatgata atattaaatc tataaaagac ttaaaaggta aaaaaattgt aggtccaaaa
     6001 gggactatac ttcatcaact gttgattgct gcacttgcta aagaaggcta tactatagat
     6061 gatgttgaat atgtaaatat ggatattcct agtgctgctt ctgcattaga aagtaaaagt
     6121 gctgatgtag caatgcttgc aggtccagtg gcattgaaaa cgataaattc aggtgccaaa
     6181 atgattgtaa atggagaagg attagtaagt ggaataatag ttactgctgt aagtgatgac
     6241 tttgctgaaa aatatcctga attagtaaaa agatttatga aggttcatga agaaacttta
     6301 aaatatatga atgaaaacaa ggatgaagtg atggatgttg tatctaaaga agttggttta
     6361 tctttagatg aaacaaaaga aatgtattct tggtatgact ttagttctaa aataacggac
     6421 aaggatataa aagaattaga agatacacag gaatttttga tgtctaatgg aatgcaacaa
     6481 aagaaaataa atattaaaga tatgttgtac aatcaaaatt agaatacttt aaaatattaa
     6541 agtggaaaat acacccttat ttattatgga aactgtatgg taaaagaggg tgtattttta
     6601 ttattataca aaaacacaat taaaaacagc ttactatttt atctacactt atgtagtcta
     6661 gtaattttct cattttctta cggtgaagta aaaacttcga atatgtgtcc tcggattgtt
     6721 ttgtgtcacc ataaactttc caatatttaa tcatagaaac ttcataatta ctatatccca
     6781 taaatccaat cacatcttta agaggatatc ctaaaaaaat acctatttca tcaggaaaaa
     6841 catcactctt aagtttatct actaaatgct ctaaataagc attaacattc gtattttgtt
     6901 tgtatcctaa aaactttaaa aagtttactg tttttttaca ttttaatttt tctgataatg
     6961 cttttttatt tacaaataag atttttaatc cctttttttc tttatcaata actataaaat
     7021 ctattttctt acacttatat agatatttat aaatatcatt aattttttcc tctctattta
     7081 catcagtaaa cgaaatattt attatttcag caggtttaga gcctaaaaag actggaccta
     7141 aaacttctaa aagccatttt atatatgatg attcaatttt atttttacaa caaaattgtg
     7201 aattacaaca agacataaaa gataacctcc ttcaaatgaa aattaatatc aagtaattag
     7261 attatactat gaatgataat caatatcaat gata
//
LOCUS       DAELTA010000176         7293 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.177, whole genome shotgun sequence.
ACCESSION   DAELTA010000176 DAELTA010000000
VERSION     DAELTA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.177"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            155..706
                     /locus_tag="KPG13_003163"
     CDS             155..706
                     /locus_tag="KPG13_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986854.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            792..1724
                     /locus_tag="KPG13_003164"
     CDS             792..1724
                     /locus_tag="KPG13_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG0986855.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            1779..2642
                     /locus_tag="KPG13_003165"
     CDS             1779..2642
                     /locus_tag="KPG13_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG0986856.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            2889..4751
                     /locus_tag="KPG13_003166"
     CDS             2889..4751
                     /locus_tag="KPG13_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0986857.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            4754..6220
                     /locus_tag="KPG13_003167"
     CDS             4754..6220
                     /locus_tag="KPG13_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0986858.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            6244..7080
                     /locus_tag="KPG13_003168"
     CDS             6244..7080
                     /locus_tag="KPG13_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0986859.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         2687 a          770 c         1324 g         2512 t
ORIGIN      
        1 cacaaaattt cagaaaacta tttacattct aaaaaaagta atgtatatta aacataatgt
       61 acattataat ggataaaaag tttctattaa attactaagt ttatttaata atcaaaataa
      121 tttatcttat attagtatgt attagggggt atttatgcaa agtttaaatg aaataattgc
      181 tgaaaattta aaaaagataa ggaaagaaaa acatcttagt ttagacaaga ttgcacaact
      241 tagtggtgta agtaagagta tgctatctca aattgaaaag tgtgaggtaa atcctactat
      301 atctactttg aagaaaataa ctaatgggct aaaaatttca tttacatcat tgatggagag
      361 gcaggaaagt gacatagaat tgattcaaaa aagtgatata gaccatttta ttgaagataa
      421 tggaaagtat ataagttatc caatatttcc atttgactcc aaaagaagat ttgagatatt
      481 tatgattgaa atagaagaag gtggaaattt agattcaaat gctgaacttc caggaacaca
      541 agagtttata actgttttta gtggcgaagt aaccattaaa ataaatggag aagattacat
      601 tgtatctagt ggaaattcta taagatttaa agctgatgta tcacatatat atagaaactc
      661 aggcaaaggt atagctaaaa tgagtatggt agtatattat atataaataa tttaatggaa
      721 ttagagtgat atttagatga ttttaggtat gaagatatat tatatttaag ttataggagt
      781 gtgatttatg gatgaataat aaaaaagcag tttttttctt ttgcagtgat ttaaagaaag
      841 acccagtagc cagtaatgtg ataaaatgtt gtgaagaaat aatggattta aatcaaactg
      901 atattgttat tgatgataat tttgtattag agtttaagga taaaaacaat aacttatttc
      961 attttgtaaa aacaaaagat gtaataagtc ataattataa atattatctt ccaattttaa
     1021 atagacattt tagagattat gattttgcag gagttgtaaa ttggcatgag ggagaaaatg
     1081 ctccagaaca tatcctaaca gctcatacaa ttggagatgt tccaacagga gaatttggaa
     1141 attcaaatcc aagatacttt aaaaatctaa tcaatgctat tgaggatata aaaaatgaaa
     1201 atctacttga tgaatttact acattaacag aagcaactca ctggtctgga acaacatatg
     1261 gtgaagaatc taaattaatt acagaatatt ctgtacctat gttggatatt gagataggaa
     1321 gtagtagtga tagttttaat aatagtatag caatacaggt attggcaaaa tcattaatta
     1381 gagtatttga ttgtgatgaa cctttgaaga ctttgttatg tgttggaggg gtacattttg
     1441 agaaatcttt ctcagatatt ataaaaaata aagaatataa catatcaatt ggacatgtat
     1501 tgccaaatca atggattgta agtggaatgt atgatgatga atctggattt gagaaactag
     1561 aaaagtgtat aaatagcatt gaaggaggaa ttgattgtat agtttttcat gataaattaa
     1621 aaggtaccta taaagagcaa tgccgtaaac taggtgaaaa acttaatgta cctgtattta
     1681 aacataaaat attaaaaaat ccaaaagact tacctatatg gtagtcttat acagttaata
     1741 taacaataag tcggattaat ttttaagcga ggtcaaaaat gatattatct tattttgcat
     1801 tattaattag agtagtgcta ttagcttttg aaagagtagt tgtaagatta ttaggtgatg
     1861 aagaaggaga tatttataaa aatatagctt catcatttct atttttcttt atagggggag
     1921 tatgtttact tccatttagc ataatggata gagttagtga ttggtcgttt ttaataccat
     1981 gttatataag tagtttagtc tattcaattg gttcagtagc gtatgtaacg tctttagcaa
     2041 ctggggaagt ttcacttgtt actccaataa atagtttaaa ttcattgttt ttactgcttt
     2101 tatcagttat atttttaggt gaaggtttaa gtttagcaaa agttgcagga atcataatta
     2161 tgattggtgg agtgtttata ttaaaaaaag taagttcttt tttaaagaat gtctctgtaa
     2221 tttttaacaa tttaccatgt agattaatgt ttttatacat atttttgcag tcagtaggaa
     2281 gagtattgga taaggctttt tttgtacaag tatctcctat attatattca actatactgt
     2341 attttttcgt aggtttaaat ttatttatct ttttagcatt taaaaagaaa caaaaaataa
     2401 tttgtgagat ttttactaat aaaaaggggc tttcaatttt aagtggtgct attaatggat
     2461 attcttattt agcattatta attgctttaa ataatctaga acttagtata gcagagccat
     2521 tttctcaagt atctatgata ataactttaa tacttgctca ttttatattc aaagaaaata
     2581 taaaagaaaa aataccaggt tcaattttga tactaattgg tgggtggcta ctgttacttt
     2641 aattgacata aaaaatgaat tataaaggtg ctattagtta gtatcagaaa atttaaaaaa
     2701 gtattgacaa ttcttttagg caaatgttaa tataataata caaaatagta taaatagaag
     2761 acatgttact ggtaatgcag gcaggatcga atatattgct ttgatttttc aaggtgttta
     2821 tataggttct gccttttttg catcttgaat ggccattcat aaaaaattat ggagaaggag
     2881 attacaagat gaattataaa aaaactgcca ttgacattat tgaacaagtt ggtggttcaa
     2941 aaaatatttt agctttagaa cactgttcaa ctagactacg ctttaagttg attgatagaa
     3001 gtaaagtcaa tcaagatgct ttaaaaaaag tacaaggtgt tatgagtata gttaatgggg
     3061 cacaatttca agttgtcatt ggaaatagtg ttgttgatgt gtatgatgaa attttaaaag
     3121 tttgcccttt agatacaaat gatagtactg taaaaactga agaaaaaata aacttaggaa
     3181 caagatttat agaatttatt attaatattt ttcaaccact gatttttgct attgcaggag
     3241 ctggtatttt aaaatcactg ttgatgttat tagcaatgtt taatatttta aaatcagatt
     3301 ctactatata tacattatta gttgctattt cagatgcaac attctatttc ctacctctta
     3361 tggtagctgt aactactgcc aatgtattga aatgtaatcg tttagtagct attgccgctg
     3421 taggatactt attgcttcca gcaacaacaa ctgctttaag tgaaggtgtt gaactatttg
     3481 gatttacaat tccaaacatt gcctataatg cacaggtttt cccggctatc ttatgtgtgt
     3541 ctttcttggc aacaatggag aaaattttta acaagtattc accaaaacca attcgaactt
     3601 tctttgttcc tatgatgtct ttggcaatta ctgttccaat tactctaact attttaggtc
     3661 ctttaggata taacgttggt acaattttta caaccattat tttattctta tataacaaaa
     3721 tgggattctt agttgtagga ttacttgctg cttttcttcc atttatggtt gcaacaggaa
     3781 tgcacaaagc actaatacct tattcaatta atacaattgg aaagttgggt tatgaagctt
     3841 tatatatgac atcttcatta gcccacaata tttctgaaag tggtgcttgt tttgcagtag
     3901 ccttgcgaac aaaagatgaa acattaaaac aaacagcatt atctgcagga atttctgcct
     3961 tgatgggaat tacagagcca gctttatatg gtattacctt acaacataaa agagcaatgt
     4021 taggtgttgt attctcaagt gcaatttctg gtaccttttt aggattattt gcagtaaaag
     4081 gatttgtaat tgttggacca gggcttgcca gtatgagtat gtttattgac ccaaacaatg
     4141 gaaacaattt gatttttgcg agtattggtt ttgttattgc aatattaggc tcgtttatta
     4201 ttacactgat tatttggaag gaagatacaa atacagtaga gtcatcaatt ggagatgttg
     4261 ttgaagaaga gctagaagaa gaaaaagaag taaatatctt agtaactcca gtagaaggaa
     4321 aagtaataga tttaagtaag gttaatgatg aactatttgc aagtaagact ttgggagatg
     4381 gagttgcaat tataccaacc aatggaaatt tatacgcacc ttgtgattca gaagttgtga
     4441 tgttatttga aacaaaacat gcaattggtt taagaacaaa aaatggggct gagattttga
     4501 ttcatattgg aataaatact gtaagtatga atggagatgg atttaaaaca tttgttaaga
     4561 caggcgataa tgtaaaagaa ggagatttat taattcaatt tgatttagac aaaatctcac
     4621 atgctaattt agattcaact gttatgattg taaacaataa tggagcagat tatgcttata
     4681 aagtgttaaa ccagtcttat ggcaatgtaa aaaaaggaag tatcttgttt gatgtaaagg
     4741 gaggaatata aatatgagaa aatttccaga aggatttctt tggggaggag ctacagctgc
     4801 taatcaattt gaaggtggtt ggaatttagg tgggaaaggg tggtctgtat cagatgtagc
     4861 aaaggcacac tttgatgcag atgtgaaaga ttataaatct aataatgaaa tcacaacaaa
     4921 agatatagaa gaaggattag cacatcctga agatgaagta aattatccaa aacgtcatgg
     4981 tagtgatttt tatcatcact ataaagaaga tattgctcta atggcagaaa tgggatttaa
     5041 gacatatcga atgagtattg cttggtctcg tatttttcca aatggagatg ataaagagcc
     5101 aaatgaagaa ggtttacagt tttatgatga tgtatttgat gagttgataa gttatggaat
     5161 tgaaccactt gttactatgt cacattatga accaccatta aatatcgttt taaactatga
     5221 tggatggtat tcacgacaag ttatcaatat gtttgttagg tatgtagaaa ctatctgtga
     5281 aagatataaa aacaaagtga aatattggct tacatttaat gaagttgatt ctatgattcg
     5341 tcatccgtac actactggtg gtttagttcg agaccgtttt aaggataaaa actttgagga
     5401 agttattttt caagctatgc atcatcaatt tgtagctagt gccattgcaa caaaaatttg
     5461 ccatgagatt attccaaatt caaaagtagg gtgtatgtta acaaaattaa cgtattatcc
     5521 atatacatgt agaccagaag atgttttggc aactcaacaa aaaatgagaa gtatttatgc
     5581 atatagtgat acgcaggttt ttggtgagta tccagtatat ttactgtcgt attttaaaaa
     5641 taataacatt caaatagtaa aagaagaaca tgatgatgaa attatgaaaa agtatccagt
     5701 agattttatt tcattctctt actatatgtc ttcttgtgag gcagctgata caactggatt
     5761 agatattaca cctgggaata cgttattagc tgtaaaaaat ccttatttag aaatgagtga
     5821 atggggttgg caaattgatt caataggtct acgtatatct ttgattgaat tatatgatag
     5881 atacagaaag cctttattta tagttgaaaa tggattggga gctaaagata ttttaactga
     5941 agataaaaaa gttcatgacc aatatagaat taattattta aaagaacact ttaaatgtat
     6001 gttagatgcg attatcgaag atggcgttga actttgggga tatacttcat ggggttgtat
     6061 tgatttggtc agtgaatcaa cgaaacaaat gtcaaaacgt tatggatata tttatgtgga
     6121 tgctgatgac tatggaaagg ggacatataa tcgatataag aaagatagtt tttattggta
     6181 taaaaaggta attgaaaata atagtataga ttttaaatga ccaatagaag gtgagcaaat
     6241 aaaatgaact acataataaa gaaagttttg aactcgagcg ttgttttagt tcatgatgca
     6301 aagtataatg aatttatttt gctagggaaa ggaattggat atggtaaaaa aacaggagaa
     6361 tatatttgtg gttccaatga taatcaaatg tttgtccctg ttgaaaatac aaaatcaaaa
     6421 caatttttag aattaatgga tgatattcct gtcgacattt taaagataac acaggaaata
     6481 atagtagagg cagaaaagtt attgaactca tcttttaata aaaacttata cttaatatta
     6541 gcagaccatt ttaactttgc aattgaaagg atgcgtaaag gaataaaaat tacaaatcga
     6601 gtattttggg aaataaaaaa ttattatcca aatgagttta aggttggaat ggttgcaatt
     6661 tatatggtag aggagagatt gggcattcaa ttaccagagg aagaagcagc taacattgct
     6721 ttccattttg caaatgctat ggcttcagat ggaaattcat atgatacgat taagtatgcc
     6781 aaagtaattg gagaaataat taatattttt gttttttctt tgaatagaac cttagataaa
     6841 aagagtatgc attatatgag atttataaca catataaaat tttttgtgga aagatttttt
     6901 tcagatagta tgttaagcag tggtgatgat ttattattta atcaaatgaa aaggtcaaat
     6961 ccaaaagaaa tgttaattgc attaaaagtt agagattttt tagaaaagaa atatggaaaa
     7021 aagttaacaa atgaggaaat agctttcttg gttgtacata tcgcacgagt taagcaatag
     7081 agtgaattac taatattatc ttatacgtct acatggtaga aaattgaaag aaggtaaagt
     7141 atataaagaa actgagacat attattttgt tgttcattat gtttatttta tgagtgtatc
     7201 ctgttaaaaa tttaagttaa tatttataat taaaatatac aaatagttaa aataagttta
     7261 tgtggttatg aatttatttt aactattttt ttg
//
LOCUS       DAELTA010000177         7172 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.178, whole genome shotgun sequence.
ACCESSION   DAELTA010000177 DAELTA010000000
VERSION     DAELTA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.178"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            46..1035
                     /locus_tag="KPG13_003169"
     CDS             46..1035
                     /locus_tag="KPG13_003169"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG0986860.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1107..1847
                     /locus_tag="KPG13_003170"
     CDS             1107..1847
                     /locus_tag="KPG13_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG0986861.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2047..3198
                     /locus_tag="KPG13_003171"
     CDS             2047..3198
                     /locus_tag="KPG13_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG0986862.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3223..3978
                     /locus_tag="KPG13_003172"
     CDS             3223..3978
                     /locus_tag="KPG13_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG0986863.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4165..6552
                     /locus_tag="KPG13_003173"
     CDS             4165..6552
                     /locus_tag="KPG13_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG0986864.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(6783..7016)
                     /locus_tag="KPG13_003174"
     CDS             complement(6783..7016)
                     /locus_tag="KPG13_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG0986865.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         2898 a          737 c         1306 g         2231 t
ORIGIN      
        1 aaaatctgtt gatagtatta tttataagta cagtaggagg ataaaatgtt tgaaaattta
       61 cctaagatag atttacattg ccacttagat ggaagtgtta gagtagaaac catgcttgat
      121 atagctatta aagagaaaat agatttacct tcaaataata tggatgaaat aaaaaaatta
      181 gcaaaggttt cttttaattg tacttcacta gatgaatatt tagaaaaatt tgatttaccc
      241 ttaaaagtaa tgcaatctaa ggaaaattta aaaagaatta cttttgaact tttggaagat
      301 gcaagtagag aaaatgtaaa atatattgaa attagatttg caccattact tcacacccaa
      361 aaaggtatga gtgttaaaaa tataattgag ggtataattg aaggaattag agaagctgaa
      421 agtatttatg atataaaagg caatttaata ttaggttgta tgagaacaat gacctcaaaa
      481 gaggcattat tagtaattga agaagggaaa tcatttgtaa ataaaggtgt tgtagcagta
      541 gacttgtgtg gtccagagaa agagggattt tgtaaggaat ataaagatgt ttttaaatta
      601 gcaagagaat atggatataa agttacaata catgcaggag aagctgcaag tggagaaaat
      661 gtattggatg ctataaatat tttaaaggcg gataggattg gtcatggggt aaaaattaaa
      721 gaccataaaa aggcgtataa tttagttaaa gataaaaaaa tattgcttga attatgtcca
      781 actagtaatg tacaaactaa gacagtagat tcttatgaag cacatccatt ttatactttt
      841 tataaagata atttacatgt aagtataaat actgataata gaactgtatc agatataaat
      901 ttaagttcag aactaaatgt tatatttgat acttttaaac taggattaga agactataaa
      961 ataatttata gaaatgcagt agaagctagt tttgcagata aagaaactaa agagtatttg
     1021 aatagtttaa tttaatagat aaaaatattt aaactttaaa ttaagctatt aaattgataa
     1081 aaatacattg tattaggaga aaaaatgtga aagattatga agatgttgtt tatattaaac
     1141 tagtcaaaaa agaacttgaa gaatggaaga aaaatatatt aaagaaacct tctatatttg
     1201 acaaagcatc aaagggtata cagaataaat ttaatggagt attgccagat aaatatcatg
     1261 aaataattac ttcttctata aaagtaatga caaaaaccgt actgtttgga tataaatatg
     1321 ctacaaagcc atcacttaga aatatgtcat tagaagaaag agaaaaatta gttttagaaa
     1381 aagctaaaac ttacaagact acagcaatga ttgaaggggc aggaacaggg gcaggaggaa
     1441 tcctcttagg tttatctgat tttcctttgc ttttaggtat taaagtgaaa tttttatatg
     1501 atgtagcttc aatatatgga tatgatgtta aagattatag agaaaggatt tatatattaa
     1561 atatattaca gttggctttt tctagtcaac agcatataaa caaaactttt aaattgatga
     1621 ataactggga tgaatatgta aatagtctac cagaagacat caatgatttt gattggagaa
     1681 catttcaaca agagtatagg gattatttag atttagctaa gtttttacaa ctaatgcctg
     1741 gaataggagc atttgtagga ttttatgtga atggaaaatt aattgataaa ttatgtgaaa
     1801 ctgctgtgtc tgcttataga ttgaggattc gagagcttaa gttataagag aaattataac
     1861 ataaaaaatt agttatacaa tatatctata tgtatctaat tttgcattga taaaaacaat
     1921 tatggataaa aggtaaagca tgatagaatc taatgtagag gtttgtattt taatggaaaa
     1981 atttagtttt tattgcttaa gatacaaatt gaattacgaa gagaagtaat tgggaggaat
     2041 attttcatga caaatttgcc aaaacaattt gatagcttca atgaagctag acaaaaagga
     2101 tttataaatg caaaggaact taaggaaagt gggaaaaaga tggttggagt tttctgtaca
     2161 tttactccag ttgaaattcc aatggcagct ggagcaactg tagtaggtgt atgcggcgtt
     2221 agtgaagagc caatacctga tgcagaaagg gtgcttccaa gaaatctttg tcctttaatt
     2281 aaatcaagct atggacatgc aataacagat acctgtcctt atttctattt ttctgatttg
     2341 ttgataggag aaaccacttg tgatggtaag aaaaaaatgt atgaagaact tgcaaaagtt
     2401 aagccaacat atgtaatgca tttgccaaat acttcaaaag gagaatttgc ctataaactt
     2461 tggaaagatg aaatgataag attaaaagaa gaggttgaga aatctttagg tgtaactata
     2521 actgaagaag atataagaac tgctataaaa gataaaaatg aagaaagaga acttttaaaa
     2581 gagttttatg cacttggtaa attacaacca tctgcactta caggccttga gttacataat
     2641 gttttatatc aagcaggttt taaatttgac cgtgcagaac ttaaacattc tttaagaaaa
     2701 gttatagatg atatgaaaga aagatatgaa aaaggtgaat gtcctgttca aaaagataag
     2761 ccaagaatat tgataacagg ttctcctatt ggtggaataa gcgaaaaaat agttaaaact
     2821 ttagaagatg caggggcatc agtcgttgca tatgaacttt gtggagctat tagatcaaac
     2881 gatttattgg ttgatgaaga aatagaagat gtatatgatg ctttaacaca aaaatatata
     2941 aatataggtt gttcttgtat gatgaataat gacaatcgta ttgaattatt agatagaata
     3001 atagatgaat ataatgtaga tgctgtaata gatgtagttt tacaagcatg tcatacattt
     3061 aatatagaaa gttatagaat aagagaattt gttacaaaag aaaaaaataa gccatttatg
     3121 tctcttgaaa ctgattattc aaaatcagat actgaacaat taagaactag atttgaagcg
     3181 tttgtagaga tgttgtagga ataaataaaa gtaggaggaa ctatgtttag tataggagtt
     3241 gactcagggt cagtggcaac aaagggtgtg ctatttgatg gtgaaaaaat tattaagaaa
     3301 ataattatac caactggatg gagccctaaa agtacatcta aacaggtcta tgagttgcta
     3361 agtagtgaaa ttgacaaaaa agatataaaa aaggtggtag gtacaggtta tggaagggga
     3421 gtaatggatt ttgctgataa gaaagtaaca gaaataactt gtcatactag aggtatttac
     3481 tttttaaaca agaacataag gactatttta gatgtaggtg gtcaagatag taaagttata
     3541 aatttagata gagatggaaa tgtatttaac tttataatga atgataagtg tgctgcagga
     3601 actggtcgat ttttagagat tacttctaac cttttaggaa gcgatattga aagtatagat
     3661 acattagcta aaggatatga accagttaat atatctagta tgtgtacagt ttttgctgag
     3721 tctgaaattg taagtcttct agctcaaaac atttctactg gagaggtagc agcagggata
     3781 cttaaatcta ttgcaaataa atccacctca atgttagcaa gaggtgaggt aatagatgaa
     3841 gttgcattta ctggagggct tgcaaaaagt aaagaactag taaaaatgat agaagaaata
     3901 ctgggtaaaa aaatttttat tgcagaagat actcaaataa taggagcttt aggagctgct
     3961 gtaataggat ttagataaaa acatataaaa ggaaaatgag aagctgaatt tattataaat
     4021 aaattcagtt ttttatttta tttatttata aaggaaaact ttggtataat tgatttaaat
     4081 gattgtatat ttgtaaatac ttgatattta gtcatatata acattataat tttaaaaatt
     4141 agctcaaaat aagggggaga aagcgtgaaa aaatttactt caaaaaaagt aacaaggttt
     4201 gttgcgctgt ttttaatatc tattttagta atgtcaacaa ttcctgtatc agctgacaat
     4261 agcaatacat tgaatcagat aaaaaaagca gaatacagtg agacgtataa acaatatcta
     4321 aatgatgcta aaaatggacg tacagaaaaa tataatggga ttataccaaa tccatatcag
     4381 ttggaaggta ctcaaatcca aagtaaagga agaacagctc caacatctta tgaccctaga
     4441 aaattaggtc ttatgacatc aataaaaaat caagaggatt taggaatctg ttgggatttt
     4501 gctgcaatgg caaccttaga aagtttctta aagttaaata attatggaga ctatgattta
     4561 tcagaagaac acttaagatg gtgggcttca gatggtgaat atggatggtc tgtaaatgat
     4621 atgaatggtg ctttaaatta tgaagctatg ggatatttaa catcttggtc aggtcctaaa
     4681 ctagaaaaag atattccata caatggtaga gtatctaaag cacaaggagc aaaaaaacca
     4741 actaatatga atacagcacc tactgttttt aatgtgacag atgctgtgtg tgtaagtaat
     4801 gacataaatt ctacaaaaaa tgctatatta cagtatggag cagtaacatc tggttattat
     4861 gaagatatta aatatcaaag tgatgaccaa aattcatatt attgtccaac aaaaagtttt
     4921 aatacaaatc atgctatttc aattgtaggt tgggatgata actattctaa agataatttt
     4981 aatgcaaata ttagaccatc aaaaaatggt gcttggttaa taaaaaatag ctggggagat
     5041 tataattctg agggtggata tttctggata tcttatgaag acaagacact tatgtctgat
     5101 atagataatt tttcaatcaa aggtggtaaa aaaccaaatg atgatgagaa gatgtatcaa
     5161 catgactatg caagtattgt acctttaatt tcaaaaaaga taacagctgc aaatgtattt
     5221 gatttcaata gaggcgatga aactctaaaa tctgtaatgt tcttaactga aagtatagga
     5281 gctaagtatg aagtatatta tgctccagta gtaaatggta tacctcaaga aaacaatatg
     5341 aaaaaattaa aagaaggtac tgcccaatat tcaggataca taacagttcc aattgattct
     5401 tttgatatac cagaaggaaa gggtgcaatt gtagtcagta tagaggggaa aaatggagag
     5461 tcaactatag gttctgagtc aaatgtacca ggatatgata catttaaagc aaaagctaat
     5521 ttaggtgaaa gttatatcat agataatact ggaaaattct ttgatataaa tagagatagt
     5581 aacttctatc cgtgtaattt tactataaaa gctgttacag aaaagtcttc tggtgagagt
     5641 atcccaaatg agtctttaat tggtagtgat agatatgaaa ctgctatcaa agttagtcaa
     5701 aatggtttca attcttcaga aaatgtagtt ttagtaaatg gcagttcaat agtagatgca
     5761 ttggcagcaa ctccttttac atctgctata aattcaccaa tattattgac acaaaaagaa
     5821 gctttaaatt caaaaactaa agctgagata cagagattag gtgctaagaa agtataccta
     5881 ataggtggag aaaattctat aagtaaagaa attgaacaac aattaaaaag cttaaatata
     5941 tcgatagaga gaatatcagg aagtgataga tacaagacaa gtttattatt agctcaaaag
     6001 ttaaatggta taaaaaatgt atctcaaata gcagttgtaa atggagtgaa aggattagct
     6061 gatgcaatta gtgtaggcgc tgcagcagca gaaaataaca taccaatcat acttgcaaat
     6121 gaaaagagcg aattgcaagg agctgatgaa tttctaaatt cattaaatat agagaaatct
     6181 tatattattg gaggaacagc atccctatca aacaatctag aaagtaagtt aaaaaatcca
     6241 actagattat ctggtagtag tagagacgaa actaattcaa agataataga taatttttat
     6301 aaaaaagata ctttaaaaaa tgcatttgta gttaaaaatg gaatcaagaa tcaaaatgat
     6361 ttaatagatg gattggcagt tggtccttta ggagcaaaaa ctgggtcacc agtaatttta
     6421 gttggtgata aattagctga tagccaaaaa gaagttctta aaaataagac tttagaaaaa
     6481 gtaactcaag ttggtggagg agcaaataaa aatgcattta aagagttagt aaaattaaaa
     6541 agtgctaaat agagtactta agtattaaca tatattagtg tgtattagta acttatctta
     6601 ataaaagctg aaaataatta cttcaaatta ttgataaata agataatcaa tgattgtaaa
     6661 gttttgtttt cagctttaaa tttatgtagt ataagtttaa aaacataaaa aagggttact
     6721 ttatatagaa atatagaaaa tcttacattt tatatataaa taacccatat ttaattaact
     6781 ttttatagtg ttctaataga tattattgaa cttaatggta aatataatgt gtaagtttca
     6841 tttaaagttg gattagattc atctatatat cttgagcaaa gtgtaattga taagctatct
     6901 gcttctataa tttcaccata tagaggacaa ctgctaaagt aagaatgtac ttcaacatat
     6961 ttatttaagt tttcttgaac ataatcgtaa aaacggtttt ttgtagtagt agccataaaa
     7021 tcaactcctt tattttttat taaagttgaa acttttaaaa agataaatat gattacttct
     7081 attagaaact aagttaaaaa ataagcaaat agatatgaaa attagaataa tatattattt
     7141 atatcttaat tataacacta gataataaaa aa
//
LOCUS       DAELTA010000178         7029 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.179, whole genome shotgun sequence.
ACCESSION   DAELTA010000178 DAELTA010000000
VERSION     DAELTA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.179"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(184..1011)
                     /locus_tag="KPG13_003175"
     CDS             complement(184..1011)
                     /locus_tag="KPG13_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG0986866.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1059..2495)
                     /locus_tag="KPG13_003176"
     CDS             complement(1059..2495)
                     /locus_tag="KPG13_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG0986867.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKVFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2582..3451)
                     /locus_tag="KPG13_003177"
     CDS             complement(2582..3451)
                     /locus_tag="KPG13_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG0986868.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3609..4262)
                     /gene="phoU"
                     /locus_tag="KPG13_003178"
     CDS             complement(3609..4262)
                     /gene="phoU"
                     /locus_tag="KPG13_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG0986869.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4290..5054)
                     /gene="pstB"
                     /locus_tag="KPG13_003179"
     CDS             complement(4290..5054)
                     /gene="pstB"
                     /locus_tag="KPG13_003179"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG0986870.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5073..5897)
                     /gene="pstA"
                     /locus_tag="KPG13_003180"
     CDS             complement(5073..5897)
                     /gene="pstA"
                     /locus_tag="KPG13_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG0986871.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5899..6816)
                     /gene="pstC"
                     /locus_tag="KPG13_003181"
     CDS             complement(5899..6816)
                     /gene="pstC"
                     /locus_tag="KPG13_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG0986872.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
BASE COUNT         2157 a         1334 c          728 g         2810 t
ORIGIN      
        1 aaatatgttt ttacagttat tctgtcttat aatgtcaata attgcaagat tattttattt
       61 taattttaat gctttctcat taattttata ttatccttac agtatacctc ttattcgtat
      121 tttttatatt atatgtttta ttttataaat aatttacttt atcttttact atatttaaat
      181 ttattataag ttttcttgcc agaaattttt aggtgttata ttttctgtaa ttggcaatat
      241 tgtatatcct ctctctttta agattcttat aatattattt aaagcattta aactttgctc
      301 tttctcatga attaatacta ctaattcatc tctttttctt ccataatata atatattact
      361 tactatctga cttgtagaag acttccaatc ctgtgtatcc aaattccagt cccaaattaa
      421 ataatcattt gccataagtt tattatatga accttctggc atatatggtt tacttccgta
      481 tggtaatctt attaattttg aactctgacc agttatttct tgtagagtat tattgcatgt
      541 attaaattcc ccaactgtta cttctgggct tttatataac ttatgtatat catgacttac
      601 actatgaaaa cctattgagt tttcttcttc caagactctc cttacttctt ccttgtggtt
      661 tttcatattc tgattaagca tgaaaaatgt cgctttgaca tcattttctt ttaaaatatc
      721 aagtagtgca tttgtatatt ttgatggacc atcatctatt gttatgtatg ctactttttc
      781 agaagtagcc cctggacctt ttgtttcttc ttgagatgct ttgtatatga tttctcctaa
      841 agtattactt tgagaaagca gttccagtgt attttcacct tttgaactta tattttcaac
      901 tggaacatta ttaatttttt cctccaataa tgttccagtt aaaattaaaa cagctataag
      961 aaatatcctt ataaatacat gaaaaacagc cttattatct ttaattttca tcccccatat
     1021 ctccctttta taaaagcata agtttaagac ttaaaaagtt attttttata tttattctct
     1081 ttcttattac tttttatatc tctactaaat atttttttaa ccttttcttc ttctttttct
     1141 cctaaagatt ctactctttt gattcccaaa gaagttaaaa attgtaatac atttaaatta
     1201 tcaaatggag tttccatatt cgtactttca ctatattcat caacattctt agtaccttct
     1261 tccacactta aaatacgctt cggacctcct acacagcctc ctacacaccc cattccttct
     1321 ataaatgtag cttctatttc tttatttaga actttcttta gaccgtccat gcactctttt
     1381 gttccttcaa ataccttttc ttcaaatttt atatttttgt caattcttct aactgataac
     1441 ttaactgctt tacttacacc tcctgttcta gcataaactc tacctgaaag ggacgcctct
     1501 actctattac tctcatccat tattgctgga tttaactcta atgcatcaaa tatttcttgc
     1561 aactctttaa atgtaagcac aaaatcaact gcatctttta tatcttctaa cattgcctct
     1621 ttcttttttg ccatacaagg acctatgaat acaactttag catctggatt taaaattttt
     1681 acagctctcc cacaagcaat cataggagaa acggatggtg atacattctc caatatttga
     1741 ggaaaactat tttgtatcaa actaacccaa ataggacagc aacaactagt tataaagcat
     1801 ccttcttcgt cctttttcat atgctcataa taatcatatg cttcttttgc agtaagcata
     1861 tctgctgcca aagcaacttc tatcatatct tcaaatccta tcttttttaa tgcagttctt
     1921 aatttaccag ggcttacatc tttcccaaac tgtcctacaa aagctggtgc aactattgca
     1981 tatacagggc tctttatatc ttttaacaat tttatcattg gtataaattc tattttatct
     2041 gaaattgcac ccaacttaca tcttgataca caatcaccac aagcactaca tacatcttcc
     2101 tcattacaat taatatgctt acttcttatc ttacatgact catcttcctt attattttca
     2161 tcacaatatt tgcaagcatc tgatattaca gatattattg gtcttttgac ttcatctaaa
     2221 tttacagctt catgtagtga ctcttttaaa tttttatctg tatcttctgt cacatccaat
     2281 cccatagtta ccctaattaa atttaccata gtatctttat caaattttcc actaaaataa
     2341 ttatttaaat tatcctttaa attttcattt aaaattttat taggtaagtt ttcaatatcc
     2401 tctatgtcat cattccacac tgcttttaca agttcgccaa aaagcttcat tttatatatt
     2461 tctagtattt tagaattgtc atttctagta ttcataaaaa atagtacctc cataaattaa
     2521 acttatatat gtttaatatt tacaaagata ctatttttta tttattattt aactactatt
     2581 attaatatga tttatttagt tttctcaaca actctatttc aatcattatt ttcattagat
     2641 gatagttgaa tacagggctt ttttcttcat ctatattcca gtctagctta tccacattat
     2701 atgttttctt aaggtattta tatgtatgct ctttcaaata aagtttattt tcaagtaact
     2761 ctatagactg catatgaaaa tgtatttctc tacatatcct taaggcattc tcaatttttt
     2821 ctatattagt ttcactcaaa ctataatcca actcatcaat aagttttgaa tatatttctt
     2881 ctaatttttc tacttcttct tcaaatttct ctacatttaa attttctttt ctaagaattt
     2941 tatcttcaat aagcaacatt gaaagttctt ctatcttttt aaactcatct ataacactat
     3001 ttagataatt tggtctagca acataataat taacaagtac tccaaacaaa acacccacag
     3061 atgtatctaa caccctatgt aaagaataat acacagggtc actatttcct acacctagat
     3121 gtatagccag aaaggttaca caggcaacta ctactgactt tattttaaac agattgcata
     3181 aatatattga gcacattact cctagaccac ataaaaatgg gtctccagct ttaatgatgg
     3241 caaatatgaa tcctattata ccacctaaaa tagtaccttc cactctattg attcccgtct
     3301 ttaaagaacc ttttactgta tcttgaacag aaattataca tgctaccaga gaataaaaca
     3361 tattctctac taaatagtca ccaagaatca tacataaagt gacactaatt cctgttttta
     3421 tagtcctcat acccatcttt tgaagtttca aagttccacc tcctaaaaag caccttatat
     3481 aaaatacgca tcctcatatt taatatgttc ctagtatatc acaatttttt aaatttaccc
     3541 attaatcata tttttttatc aattctttct taatttagac aataaaaaaa ctgctttaaa
     3601 gcagtttttt atcctatctc aaccatttta ccctcaattg caaaaattat cttctcacat
     3661 acatttgtta tatggtctcc aattctttca aggtatcttc ctataagtag aagttttacc
     3721 ccttggtcta tggtatttga gttttcatgc attattttta aacaatcaat ttgtacttgt
     3781 tcatacaatc catctacaat atcatcttta gttccaacat cataagctat acttgcatca
     3841 aataagatta aagcttctct agtctgagac atcatagact tacattcttt tgccatctta
     3901 ggaatatcta ttagctcttt tatataagcc tctccaccta tcttcaacat ttctctagct
     3961 atatttgctg cataatctcc tattctttcc agctctatag atatattacc taatgaataa
     4021 ataaatctta agtcccttgc cattggttgc tttaatacta ttagctctat actcttatct
     4081 cttatttctt ctcttaattt atttatttct tcatcatctt ctactacttt tcttgcaccc
     4141 tctaaatcct tctctatcat acaagtcaca gacttttcta ttgcttcttc acatttttcc
     4201 atcataagca ctgtttgttc ttttaaagta tcaatactta tatctaagct attattgacc
     4261 attgtctaac tcctttatag ttatatattt taaccaaatc tacctgttat atagtcttct
     4321 gttcttttat ctacaggagt ggtaaacata gtttttgtat cgctaaattc tataacctct
     4381 ccatttaaga aaaaagctgt ttcatctgat atacgagcag cttgttgcat attatgtgta
     4441 actatgacta ttgtgtagtc tttttttaaa tcctctataa gttcctctac ttttaatgtt
     4501 gaaattgggt ctaaagcaga tgtaggttca tccataagaa taacttctgg tctcatagca
     4561 attgctcttg ctatacatat acgttgttgt tgaccaccag aaagtcctag tgcagatgat
     4621 ttaagcctgt ctttaacctc atcccaaata gctgcacctt ttaagctttc ttctacaatt
     4681 ttatctaatt gcttcttatc tcttagtcca tgtgtacgag gaccatatgc cacattatca
     4741 tatatactca taggaaatgg atttggcttt tgaaatacca ttcctacttt agtacgaaga
     4801 tttataacat catctgaggt atatatgtcc tctccatcaa cagaaatgtt tcctttaatt
     4861 gttacatctt cgattaagtc attcattcta tttaacgttc ttataaaagt tgattttccg
     4921 catccagatg gacctattaa agcagtaacc ttattctctt ttatatccat atttattttc
     4981 tttaatgctt gcttatcacc ataaaataaa tccaaatctt ttacactcat ttttatctta
     5041 tcgattaatt ccattattca cacctcttaa atttaataat tagctttatt taatttctta
     5101 gcaacaaatc ttgctagcat gtttagtgct aatacaatta ctattaaaac aattcctata
     5161 ctggctgctg ttgctatatc tccactctct ttagtcagta agtatgagtg tacagttaat
     5221 gttcttgcac tatcaaatat tccacctggc atcttggcta cagtacctgc tgtaagtaat
     5281 attgcagctg attctccaat tatacgacca actgataaga ttactccaga taaaattcct
     5341 ggtattgcac ttggaagtac taccttatat aaagtttgaa attttgtagc tcctaatgcc
     5401 aatgatgatt ctctataagc ttgaggaact gtttttaaag cttcttctgt tgtacgaatt
     5461 attactggta atattattat tgcaactgta agtgaacctg caactatgga atatcccatt
     5521 tttaatgtaa ctacaaagaa aattcctcca aataatccat atactataga tggtatccct
     5581 gataaacttt cagttgcaaa tcttatagct gtaactgctt ttcctttttt agcatattct
     5641 tgtaaatata tagcagccaa tattcctata ggagttgcta caactataga aactactact
     5701 gtatataatg ttgtaattat cattggaagt ataccaccat cacctgatgc tgaataattg
     5761 ctaaataaga aattaaaatt catgttgcca attcccttta taaatataaa tcccactata
     5821 actactaatg atactattgt aaatagtgct gatagatata ctaatgactt caatagattc
     5881 tctttgaact ttctcatctt aatttaccgc cttatttgaa agtttattta aaactaagtt
     5941 taaaattaat ataaatgtaa acaatactac tccagttgca aatagcattt cttgatgtgt
     6001 accaaatgcg tatcccattt caagtgctat attagttgta agtggtctta cactatctgt
     6061 tattgcagtt ggcataactg gtgagttgcc tgcaacaagt attactgcca ttgtttcacc
     6121 taaagctctt cctactccta acactactgc ggctaatact ccagattttg cagcaggtaa
     6181 tacaactttg aatattgttt ctatttttga tgcaccaagt gctaatgaac cttctttgta
     6241 tgcttttggt actgctctta ttgctgtttc tgatacagat attattgttg gtaacatcat
     6301 tactgctaat actattacta ttgctaacaa actttgacct tttggaagat taaatatact
     6361 ttgtatagtt ggaactatca ctgcaagacc aaatacacca tataatactg aaggtatacc
     6421 tgctaaaagt tcaactgctg gtgacattat ttttgctaat cttttaggtg ctacttctgc
     6481 tataaataca gaggttaata taccaacagg tactcctata acaagtgctc caagtgttgc
     6541 aaatattgat gctactatca tagatgatat tccaaattta ttggcagaag gaacccaatc
     6601 tgcaccagtt aaaaagtcta taaatgaata tccttcaaat ataaatggtc ttaatccttt
     6661 ataaaataca aatcctatta tcaaaagtaa acttgcaact gctactaaag cactaagtag
     6721 aaaaatattt tttgctactt tttcaattac gtattttgtc ttattgccat tattgtcatt
     6781 tttttctaaa cctactaatc tagattttac tatcataata ccctcccatt aaaatatact
     6841 tatcgatttt cgttctgtat tcaaaattta attttttata attcgtcttc atcaaatttc
     6901 ctgtactttt attctacttt ttttaagtta aatctatatt aggattaagt taagcaagtg
     6961 taaaattata tttacttttt aacattaatt ttacttttaa aactatattt gacatttact
     7021 ttacaatat
//
LOCUS       DAELTA010000179         7018 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.180, whole genome shotgun sequence.
ACCESSION   DAELTA010000179 DAELTA010000000
VERSION     DAELTA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.180"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            47..823
                     /locus_tag="KPG13_003182"
     CDS             47..823
                     /locus_tag="KPG13_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0986873.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(915..1667)
                     /gene="xth"
                     /locus_tag="KPG13_003183"
     CDS             complement(915..1667)
                     /gene="xth"
                     /locus_tag="KPG13_003183"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG0986874.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1828..2778)
                     /gene="galT"
                     /locus_tag="KPG13_003184"
     CDS             complement(1828..2778)
                     /gene="galT"
                     /locus_tag="KPG13_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG0986875.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3163..4359
                     /locus_tag="KPG13_003185"
     CDS             3163..4359
                     /locus_tag="KPG13_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0986876.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4694..5146)
                     /locus_tag="KPG13_003186"
     CDS             complement(4694..5146)
                     /locus_tag="KPG13_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0986877.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(5457..6902)
                     /locus_tag="KPG13_003187"
     CDS             complement(5457..6902)
                     /locus_tag="KPG13_003187"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG0986878.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
BASE COUNT         2382 a         1109 c          845 g         2682 t
ORIGIN      
        1 aatctatagt aaaagaaata ataaaaaaat ttaggagata aactttatgg aatacataat
       61 tttcgatttg gaatttaacc aaggatttga taaaaaactc aataaaactg tatccaatga
      121 aaagtgtcct tttgaaatta tacaaatagg agcaattaaa ttggattcta aattcaatat
      181 aatagatacg tttaatagct atgtaaaacc tacaatttat aaagacatac atccctttat
      241 tagtcgtatg actaatatta aaagttctga ttttaatgat tcgcctactt tccctgaggt
      301 ctacaacaat tttataaaat ttatatcatc acaagaccct atattatgtg tttggggagc
      361 tggagatttg aaggaattat acaggaatat caactatcac aagctaccat caaattcctt
      421 acctaaatct tatattaata tacaacaaca tgcatctaaa tatttcaata atccagcagg
      481 aaaaagcatt ggcctacaaa atgctatttc tatattggaa cttgatgaaa aaatgtctta
      541 tcacaatgct ttaaatgatg catattatac tgctaaagtt tttattaaga tatacaatcc
      601 aagtatagtc ccagatatat atctttatac gagtataaaa cctaaaacta taagatattc
      661 taacaaaaaa agagttgatt atgataagtt atttgatgaa tttaggaaaa ttttaaatcg
      721 tgaattaact aaagacgaga aaaaaatcat taacttagct tataatatgt ggaaaacaaa
      781 tcaatttaca ttagaaaatg ttaagcaaag aaaaaacaaa tagagtttat ttagataaaa
      841 aataggcatg tagattaaaa aactagttaa ctttaaattt atatctacag cccaattttt
      901 atcataatta atttttattt tatctctaat tcaactggac aatggtctga acctaatatt
      961 tcagtgtgta tatccgcact tactaatcta tcttctaatt ttttggatgc acaaaaataa
     1021 tctattctcc accctgcatt attttttctt gcattaaatc tatacgacca ccaactataa
     1081 acaccttctt tatcaggatt aaaatatcta tatgtgtcta taaacccact gtctaataat
     1141 ttagaaaact tctctctttc ttctttagta aaacctgcat ttttcatatt atttttagga
     1201 ttttttaaat ctatttcttt atgtgcaacg ttcatatctc cacacacaat tacaggctta
     1261 tgattatcta gtttggttaa ataatctata aagtcgtctt cccatctagt tctatattct
     1321 agtctcttta attcagattg agaatttggc gtatatactg taacaaagta aaaatcttca
     1381 aactctagag ttatcactct accctctttg tcatgttctt ctatatttat tccatacatt
     1441 acttttaaag gctctttttt tgtaaatata gcagtacctg aataaccctt tctctcagca
     1501 taattccaat attgaaaata tcctggtaaa tctaactcta tttgcccttc ttgcaattta
     1561 gtttcttgta aacaaaatat atctgcatct acttctttga aaaagtctaa aaatcctttc
     1621 ccaacacaag ctcttattcc attaacattc catgatataa atttcatact tatactcctc
     1681 atctaaacat tgacacactc cccatagcta aagatggggg cttttcaaca attttattgt
     1741 aaattcttaa tttactttta ttttaacaat gctatactat aatttcatct aaaattacta
     1801 tttacttaat acctaaaaat atatgtttta atcaattttt aacttttttg ttaaatcttc
     1861 agggctcatt gaagaaacat acatatttgt tccaagttca aatcctccaa aattatattt
     1921 tcttgggatt atgtgcatat gaacattaaa atacgatttt gtttcaatct ttgttggatg
     1981 agtgtgaata cataaattaa atggcatata tccattgata ttgtataact tcttaaacaa
     2041 cttttcaaat attgtagaaa gctcctgtat attattatca tttaactcac caaactctat
     2101 tttatcttta aatattattc ttacttctcc actatacttg gttgcatatg gtataagtac
     2161 caaaaaagct tctccattat aaacaactct tctatctaaa cttatttcat tttctattaa
     2221 atcatcatac aaagagcttt tattgctgtc ataatactcc tttgaaacca acagttcatt
     2281 cgtaatttca ggtggaataa aagacataga caatatttga gcatgtggat gcatcaaaga
     2341 tgctcctgcc tttctcaaga agtttttgaa gatactaaca tactctactt tatcatcttt
     2401 cgacaaatct ctaaatctat ttctatacat aaaaaaaaca tcttcaaatt ctttttgact
     2461 catgttataa aaactacgat tatgtttatc gctctctatc attacttcat gaacaccata
     2521 tacatctctt gccatatcat caattatagg aaatttattg tagacagatt ttacaagcca
     2581 tccttcttca ctatctattt caaataatgc atcagttgca tgagcttcat ttcctttgca
     2641 aaatgggcat tcttcatcat attcattagt ttcttcatct tcatttggga tatctgctgt
     2701 atctaaaggt ctttttaacc tatctgtagc aaatataaca acatcattgg taattggatc
     2761 tattcttaat tctttcataa ttaacacctc ttagttaata gcctttaata taattatatg
     2821 atatttatcg tatctgttaa ataaatttat aatatttttt ataattttgc atatttatta
     2881 ttatatattt tgtctatagc tgaaagatta taactaaaaa atcactatca gtacatcaga
     2941 atattatctt actataaatt aatttatgaa tacataattt agtggcataa acatgggtat
     3001 tttactataa tttgttaaaa gtttatttta aagctatatt aatttttttt gatgattttt
     3061 acccataagt atgttattat tatcttgtaa agatgtcttg tattcaaaca tacaatttac
     3121 ataatacaat atacgtattt aaattaaata gggggtatta aaatgaatta ttcaaacaga
     3181 gttagtgcaa tgcaagcctc tccaatcaga aaacttgttc catttgcaca agcagcaaaa
     3241 gataaaggaa tcaaagtata tcacttaaac ataggacaac cagacataaa aactcctaaa
     3301 ggattttttg atgctgttaa aaactttgat agcgaagtct tagaatatgc tacatcagaa
     3361 ggtattccag aattattaga agcacttcaa aattactata aaacttacga tatgaatttt
     3421 gaaaaagatg aattattagt aactaacggt ggtagtgaag ctttactttt cactatgatg
     3481 gcagtttgtg acccaggaga taatctatta gttccagaac cattctatac aaactacaat
     3541 ggatttggtc aatctgtaaa cgtagaagtt aatgctgtta caacaaaggc agaaaacggt
     3601 ttccaccttc catctaaaga agaaatattg tctaaagtag atgataaaac taaggctata
     3661 attctttcta acccaggaaa tccaactggt gcaatttata caaaagaaga acttaatatc
     3721 ctagctgaaa tagctaaaga aaaagattta tggataatag cagacgaagt ttatagagaa
     3781 tttgtttatg atgggcttga gtatacaagc tgtggtaact tagagggtgt acaggataga
     3841 gttataataa tagacagtgt ttctaagaga tacagtgctt gtggtgctag aattggttct
     3901 atcgcttgca aaaataaagg tcttatcgct caaatactta aattatgtca aggaagactt
     3961 tgtgttccta cattagagca aataggagct gttgagttat acaaaactcc tgtttcttac
     4021 ttcaaagaag ttaatgaaga gtacaaaaaa agaagagatg tattatacaa tgaattaatg
     4081 aaggttgaag gtgttatatg taaaaaacca actggtgctt tctatatagt tgctaaatta
     4141 ccagttgaaa atgcagaaga tttcacaatt tggatgttaa aagaatttaa taaagataat
     4201 gaaacagtta tggtttgccc tgctgaagga ttctatgcta ctccaggttt aggacgtgat
     4261 gaaataagat tagcttacat cttaaatgaa aaagaccttc atagagctgc tacattatta
     4321 aaagaaggtt tagagcaata tgtagcttta caaaaataat tttttaaact taatttatat
     4381 aaatttaatg atataaaatg tccccttttc agatattatt tgaaaagggg acatttctta
     4441 attaatatat taattaagac ttagacctga ctatttttca ttttctttat atagtgttgg
     4501 ccagtaatct ttattcactt taattaaagc atctaaaact tgtcttgcct tagttagaga
     4561 aattataaaa aagtacatca agaaaattta agatgtactt tttagattgt atttagactt
     4621 ataattatat agttaaatga ataacaaaga attaatctta gtaccattaa atacaaaaat
     4681 ttttatattt tttctatcta aattcttctt tgtttaatat atattgaaga gtatttattg
     4741 cttctaaata tttttttctg tcttcttcag ttaatttaga aagtttttct cctaaaccat
     4801 cacttaattt atccataaaa tcagttgcta tcttatctcc ctcttcagta atgtcaatta
     4861 gatattttct tctatcaact aaatctcttt tccttgttac atacttctta tcagttaaat
     4921 catcaaccat actagttaaa ctgccttttt ctatatttaa cttagagcat aaatcagtca
     4981 tacttattac tccataattt tttatgaaaa ctaatgctct taattgtgtt ttatttacat
     5041 tataatctgg tgcaaatttt tttaggtctt ctaagtacag gctagaataa atcttaggaa
     5101 atgtttttac taaaaaactt atggtatctt ttactatata gttcatataa tcacctcggt
     5161 tgttttaaaa tttattaaat tatctaaata actttatttc agtttacatt aaaaatattg
     5221 caagtacaat agtttttgct gaatgtttaa atttaattag taatcttatc atttttttat
     5281 ttttatttat attattccta ttaaatatta tttatatagc tattttatta aattcatggt
     5341 atagtaagta aagaagaact cttcacagcg gtggagagaa gacgctagcc cattaaaatg
     5401 gctatcttat ttataaataa gatagccgtt tttaattata ttttatctgt acaattctat
     5461 actcttaggt ctacatctac ttttcctaag tcttccttat aatcttttat agcaggttct
     5521 acaacttcat ttacaaattc tacaacttgt tctggagctc tacctataaa gttttttggg
     5581 tccattatag atagtatttc ttccttgctc attttaaatg aatcatcatt cattataagt
     5641 tcaattaagt tattatcctt gccttcatgc tttactctat aggcagcttt catagaatat
     5701 tctcttatta tttcatgcaa ttcctgtcta tctccacctc tttttacagc ttccataagt
     5761 attgtttctg tagccataaa aggaagttct tcatttacac gtctatttat catattttca
     5821 tatactacta aaccatcagt tacatttatc cctatttcaa gtatagcatc tgctgccata
     5881 aatgcttgag gtattgccaa tctcttattg gctgagtcat ctaatgttct ctctaaccat
     5941 tgagtggctt gtaccattgc agggcttata gattcagaga ttatatactt agataaagat
     6001 gctattctct cacttctcat tggattcctc ttataagcca ttgctgatga acctatttga
     6061 tttttttcaa atggctcttc taattctttt aaactttgta aaagtcttat atcattagtc
     6121 atcttatgca tactttgagc tatccctgat agtatgctta ttacttgata atctaacttt
     6181 ctagtatatg tctgtccact tactgcatag gaagaatggt atcccatctt tttacaaatt
     6241 ttcttatcta actcttttac tttttcgtgg tctccttcaa ataagcttaa aaaacttgct
     6301 tgagtaccag tagtaccttt aactcctctt aacttcatgt tgtctattct atagtttaaa
     6361 tcttccaaat caagaattaa atcttgagcc cataaacaag ctctttttcc tactgttgta
     6421 agttgagcag cttggaagtg tgtaaatcca agagctggca tatctttgta cttaagtgca
     6481 aattctttta aattgtttat taaatttaca agctttactc ttagaagttt taaagcttca
     6541 ttcatttgta taacatctgt attatctcca acatatgcag atgttgctcc taaatgtatt
     6601 attcctttgg ctttttcaca ttgtaaacca taagctttta cgtgagacat tacatcatgt
     6661 cttacttctt tttctatttt tctagcttca tcaaaattta tattatctat attagctctt
     6721 agctcatcta attgttcttc agttatattg attcctaact catgttcacc ttctgcaagt
     6781 gcaacccata accttctcca tgtagagaat ttaaattgtg gagaaaatat ataactcatg
     6841 tccttgctac aatatctgtc tgtaagtggg tttgagtaag tagtatattt attatctatc
     6901 attatattag caccttcctt aaatcttttg tcgtgtgatt actgtgtaca cattagtaca
     6961 taaataatta tatataattt tacgaactat aacaagactt ttttattttt tgttcaca
//
LOCUS       DAELTA010000180         6909 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.181, whole genome shotgun sequence.
ACCESSION   DAELTA010000180 DAELTA010000000
VERSION     DAELTA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.181"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            6..2183
                     /locus_tag="KPG13_003188"
     CDS             6..2183
                     /locus_tag="KPG13_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG0986879.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2197..2619
                     /locus_tag="KPG13_003189"
     CDS             2197..2619
                     /locus_tag="KPG13_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0986880.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2793..3368
                     /locus_tag="KPG13_003190"
     CDS             2793..3368
                     /locus_tag="KPG13_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG0986881.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3493..4209)
                     /locus_tag="KPG13_003191"
     CDS             complement(3493..4209)
                     /locus_tag="KPG13_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0986882.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            4374..5099
                     /locus_tag="KPG13_003192"
     CDS             4374..5099
                     /locus_tag="KPG13_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0986883.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5159..6751
                     /gene="hcp"
                     /locus_tag="KPG13_003193"
                     /gene_synonym="priS"
     CDS             5159..6751
                     /gene="hcp"
                     /locus_tag="KPG13_003193"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG0986884.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
BASE COUNT         2699 a          785 c         1244 g         2181 t
ORIGIN      
        1 aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa aatgttaata
       61 tggatagtta taaacaactt aaagctaaaa taccctgtaa ggaaacaggt atagaaataa
      121 agaggacttt atgtgatatt tgttcgccag gagtacattg tggaattgat gcatatgtta
      181 aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat aaagggttgt
      241 tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga ataaaaacac
      301 ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca tgggatgaag
      361 cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga gcaaagcatg
      421 tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg tttgcatctt
      481 catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca gcacagatgg
      541 ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat gcaaaaactt
      601 atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg aatctaataa
      661 aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca ccgacaactc
      721 aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct ttagctcttg
      781 gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata gaaaagcatg
      841 tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat actgtaagta
      901 gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg tcacaaggtc
      961 cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga tttcaaaatt
     1021 atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata ggtggaaaca
     1081 taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta gaaaaagagt
     1141 ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa agatttcctc
     1201 tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa atagaagaaa
     1261 agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga atgtttccta
     1321 attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat atagatttat
     1381 ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca tcatttgaga
     1441 gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa gttattgata
     1501 aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat gctatgaata
     1561 tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg tttaaaaata
     1621 catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt aaagatttta
     1681 agccatatat agtaggttca tatacaagag aagggtatga tactagcact tctaggtttg
     1741 aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca ctaccaacat
     1801 atagaggatt taatgataat atagatgatg aatatactca tttattgaca tcaggagcaa
     1861 gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga atattaaaaa
     1921 atgaagctaa ttgtgatatt tctagcttag atgcaaaaga attgggtgtt atcagtggag
     1981 attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat gttactgata
     2041 gagtcttata taagaatata catatgtatc atggatatag agaagctgat ataaatagta
     2101 ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt tgtaagtgta
     2161 agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt taaatttaat
     2221 aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca aaatgataat
     2281 aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg tgaaaatata
     2341 gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg tgtatgtcct
     2401 aaaaattgtt ttaaaaaaga agaaggattc attgtattag acaatcaaaa ctgtataggg
     2461 tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa tgatggtaag
     2521 gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc accttgtgta
     2581 atggtatgcc ctacaggagc attaaaattt aataaataat tgtattaaat tgctatacta
     2641 cttttaaata attgtgttaa gaattatttt ttgatatact tttgtaatat aaaatgtaaa
     2701 taaaacaaat acaaagtata taaaatatat ttttagaata aagtgtgctt tagattaaat
     2761 ttagaaaaat aaattagaca ggagaactta atatggtaaa agtattagcc tttatgggca
     2821 gtccaagaaa aaaagggaat gtagacacaa tacttgatga gataataaaa ggtgtaaatg
     2881 ataataatgt tgaagttaaa aaatattatc taaatgatat gaatattaaa ggatgtcaag
     2941 gctgtctata ctgtagaaaa gttcctactt gtgcatttaa agacgatatg attgacatat
     3001 atgaagatat aaaaagtgct gaatatataa taattggttc gccagtatat atatgtcagg
     3061 tatcagctca aacaaagcta cttttagata gattgtatcc tttaacagag ataaataagt
     3121 ctaaacatat acctaggttt ggtcaaaaaa agttaatcat ggtctatact caagcagctc
     3181 ctatttcgtt tttatttaaa aaatatttta agtatactgc aaaacattta aagggcatgg
     3241 gattaaatca ctacaaaact atagttgtaa ccaaagcatt tgaaagaaat tccactaaaa
     3301 aaaattttaa ggtattgaaa aaagcctata aacttggaat gaaaatagcc attgatatca
     3361 atggatagat aaaagtttaa tttatagtat agctaaagga gcattaatta ttaatactcc
     3421 ttttttgtat aaaggtttct agaaaataac taataaaaaa caatattatt tgaatacaga
     3481 tattatacaa gcctagatat ctaacttgtt ttgaaaataa aagatttcaa atttattttt
     3541 gttaaaagat aacaagcctt catctcgcat cttgccaagc tctcttgaca tagaacttct
     3601 gtcaacacat aaaaaatcag ccatttcatt tcttgaaaag ggaattgtaa actggtggct
     3661 acctgtttgt tctatctgtt ttgaaaaata cataagcaat ttctcgcgag ttgttttacg
     3721 agaaagtaat tctattttat tgtgaagtaa aatgtttttt tcagctatta attgtagcat
     3781 attttcaaca agcctattat gaaatacaca tgcagatgaa caaataccag ttacactttt
     3841 aaactttata aataaaactt cgcaagaaga tgttgtggtt actgtaacat tacttttatg
     3901 tagtctagta catgcgaata cctctccaaa taaatctcca ggcaagagtt cagctacaat
     3961 attcctattc ccttcgatat cttcctttat tatatgagca cttcctttaa gcataattcc
     4021 aatagaagta acttcacttc caacagagaa tattatacta tcttttgggt aagtcttatc
     4081 tacaacacct aaacattcca acatttcttc aagttcatca cttttaattc cttcaaatag
     4141 aggaattttt ttaagtaagt ctatatgctt ttctaaaaat tttttcaaat aatcacttcc
     4201 ttgttgcata tgcaaccgat aatttgtttt cattgtacta tactaagttt ataaaagcaa
     4261 ggggatataa aaatcctact acaagaaatt tgaaataaag aaatataggt attaaaaata
     4321 aatatataag aaatcaatac tttaaattaa aaaaatttta tgaggtgata taaatgatga
     4381 gaaaaataat taaaattgat gaggaaaagt gtaatggttg tggactatgt gttgaagctt
     4441 gtcatgaaga agctatagga atggtaaatg gaaaagcaaa acttttaaga gatgactatt
     4501 gtgatgggct gggagattgt ctgccaactt gtccaacaaa tgcgatttca tttgagtata
     4561 gagaagcagc agaatatgat gaagctgcag taaaagcaaa tatggaagca aagaaagcac
     4621 aaaagaaaac tttagcatgt ggatgtccag gttctcagtc taagtcaata aatagagagg
     4681 tttcaaatag tacgtctata agcaatgata tagtagagat aaaaggttct caacttaatc
     4741 aatggccagt acaaataaaa ttagtaccaa ctaatgcatc atatttaaag aatgcaagtt
     4801 ttttaatagc agcagactgt acagcgtatg catatggaaa ttttcataat aaatttatga
     4861 aaaataaggt tacattaatc ggatgcccta aacttgatga agttgactat gctgaaaaac
     4921 ttacagagat tttaaaagaa aatgatatta aaaatatagt agtaacacgt atggaagttc
     4981 cttgctgtgg tggtatagta aatgctgtta agacagctct acaaaatagt ggaaaaatga
     5041 taccatggca aatagttact atatctgtag atggaaaaat tgtagatgga gaaatttaat
     5101 taagtgaatt atagaaaaat ataataaatt ttaataaata aaaagggagg aataaaaaat
     5161 ggaaaaaatg ttttgttttc aatgtgaaca aacagcagga ggtaaaggtt gtactataaa
     5221 tggtgtctgt ggtaaaaagg ggggtacagc taacttacaa gacgaactta caggagtgtt
     5281 gattggtctt tcaagagcaa ctattggaaa taagaataga ccaacaagtg aaacagataa
     5341 aattatgata gaggggctat tcactactat aacgaatgta agttttgatg atgaagctat
     5401 aaaaagacaa attgaaaaaa ctgaggtaga aaaaagtaaa cttgtaccta gatgttcaga
     5461 ttgttcaaca acttgtaata gaaatgataa ctatgatatg aaggatttat ggaatgataa
     5521 tgaggacata cgttcattaa aatcattaat cttatttgga ttaagaggta tggctgccta
     5581 tgcatatcat gcaatggtac ttggttatac agacaaagac gtaaatgaat ttttctatga
     5641 aggattaata gcagttggag gagacttgtc tattgacgaa cttttaggtc tagttatgaa
     5701 aactggagaa ataaacttaa aatgtatgga attgcttgat agagcaaata ctgaaactta
     5761 tggaacacca gaaccgactc aggtatctat gaaaatcgag aaagggccat ttattgtagt
     5821 tacaggacat gacctttatg atttacacaa attattagaa caaacgaaag ataagggtat
     5881 aaatatatat actcatggtg agttattacc agcacatgca tatccaaaac taaaagaata
     5941 taaacatcta aaaggtaatt ttggtacagc atggcaaaac cagcaaaaag aatttgataa
     6001 tattcctgct ccaatattat ttacaacaaa ctgtttaatg cctgttaaag atagttatag
     6061 agatagagta tttactacag aagtagttgc ttatccaggt atggttcata ttgatgaaga
     6121 taaagatttt tctagtgtaa tagaaaaagc tcttgaactt ggaggatata gagaagatgt
     6181 tcatatgact ggtattaatg gaggagatac attaactact ggttttgcaa gaggtacagt
     6241 ttcatctgta caagataaag tagtagatgc tgtaaagagt ggagctataa gacacttttt
     6301 attggttgga ggttgtgatg gtgcaaaacc tggaagaaat tattacactg aatttgtaaa
     6361 acaagctcct aaagacactg ttatattgac acttgcatgt ggaaaatata gatttaatga
     6421 tttagacctt ggggatatag gtggcttacc tcgtttaatg gatatgggac aatgtaatga
     6481 tgcttatagt gcaattaaag tggcagtttc attggcaaat gcttttgaat gtggagtaaa
     6541 tgatttgcct cttagcatgg tactgtcatg gtatgagcag aaagctgttt gtatacttct
     6601 tacactactt catttaggta tcaaaaatat ctatataggg ccttctttgc cagcattctt
     6661 atctaagaat gtattagata tattggttga taaatttgca ttgacaccta tatctactcc
     6721 tgaagaagat atgaagaaga ttttaggata aataaacata tctatactta aagatattta
     6781 tagttaaaat atttttaagt atgccacaat taagataagg ggttaaactt aaaatttgat
     6841 taaaaaatca attttgagat gaccctttat ttattttttg taataattga aaaataaacc
     6901 tgctttcag
//
LOCUS       DAELTA010000181         6877 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.182, whole genome shotgun sequence.
ACCESSION   DAELTA010000181 DAELTA010000000
VERSION     DAELTA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.182"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            218..1378
                     /locus_tag="KPG13_003194"
     CDS             218..1378
                     /locus_tag="KPG13_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG0986885.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1720..1881
                     /locus_tag="KPG13_003195"
     CDS             1720..1881
                     /locus_tag="KPG13_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986886.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2074..2703
                     /locus_tag="KPG13_003196"
     CDS             2074..2703
                     /locus_tag="KPG13_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG0986887.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2942..4201
                     /locus_tag="KPG13_003197"
     CDS             2942..4201
                     /locus_tag="KPG13_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG0986888.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4460..5455
                     /locus_tag="KPG13_003198"
     CDS             4460..5455
                     /locus_tag="KPG13_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG0986889.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5701..6768
                     /locus_tag="KPG13_003199"
     CDS             5701..6768
                     /locus_tag="KPG13_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG0986890.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
BASE COUNT         2723 a          672 c         1182 g         2300 t
ORIGIN      
        1 aaataattgt tgaaaaagat tttaaattca gattattcat ttctaaagta attaatgtgc
       61 aaatctttat taatatatgt caatagttag gtatatatgt atttaattag catgatatag
      121 ataaaagtat ttattttaat tgtctattat ctgaaaaagt ctttacttag aaatcataaa
      181 aatcaaatta attaggaaaa gaaagggtgt gtataatgtg attgagactg gatttgtata
      241 ttgtagtttc ttggttgtat tcgtaggtgt aatttttttt atggttgata aattgaaatg
      301 gaaggtattt gatgttatta atccaatgct tttaatatac ttaggttcag ctatacttgg
      361 atcatttggt ttatttgcac agaatgaaga tgttggaacg tatcaaaaca taataacagt
      421 gaacttcctt ccattaatgt tagtgttttt gttaattcaa tgtgatttaa gaaaaatatt
      481 aaagatgggg cctaaaatgt tattatcatt tttctgtgct acatttacat ttatgcttgg
      541 atttgttata gcattcataa tttttaaaaa ttcaatgggt gcagattcat ggaaaattct
      601 tggggcagca tctggttcat ggataggtgg ctcatcaaat atgattgcag cagctaatgc
      661 tctaggagtt gcagaagaag gacttagtta tgcaatactt atgggttcta taggatatac
      721 agtatggatg tctgtttgtc taatgtctgt gaaatttgga cctaaattta acaaatggac
      781 aaagtgcgat acaagtttta tagatgaggt tacatcaaaa cttgaaaaag aaaataaaga
      841 taatacagca cctacgtttg tagaacttat gattttactt tctgttgggt ttggagttgc
      901 gtcttttgca caaattgtat ccaagatact tccaacttca ggtatactta atgaaactat
      961 gtgggtagta cttgttgcat ctacagtatc aatattattg ggtatgacaa aaatatcaaa
     1021 gcttaaaggg agtacactgg taggaaatgt gtttatgtat ttactccttg cagctacagg
     1081 tagtaaagca aatttctttg gattgtcaga agcacctgtt tacataatgt ttggattggt
     1141 agtagtcctt gtacatgcta tttcatttat agggcttgct aaactattta aacttgattt
     1201 atttacttgt gaagtaggaa gtatagctaa tataggagga gtaactacag ctccaatgat
     1261 tgctggggtt cacaatcctg tattaatacc agtaggtgta cttatggggc ttctaggaaa
     1321 tattataggg acttattgtg ctcttatggt aactcaaata ttacatatgt tagcttaata
     1381 aaaatatggt ataaaattaa taatttaaat aagaaaagtc atttcaattt aagcagaagc
     1441 ttttactgaa atggcttttt tgtttgacaa gataatctat ttcaagaaga tgttttgaca
     1501 tatttagtgt ataatattgt aaaatttact aaaaacgaga tatatttaat ttttatattg
     1561 aatcgaacgt ctgtatgatg tataataaat atacagaacg aatgtttgca gaacgggtgt
     1621 tttttgaatt tattttaata atttatgaat atatatcaga atgcataaat atatttaaat
     1681 ataaagatta aaaagaattt aaaggagtgt tgataactta tgataattcc atcaagaaga
     1741 atacaagata taacattaac aaatttaaaa aatggtgaag taactttaat tgaattggat
     1801 gaaatataca agaagatggg atttttattt gttgttagtg aaggcaaatt gaaaaagata
     1861 aaaaaggaga atcatcatta gaaatgaatg aaataataaa tattataata gtttataaaa
     1921 cctaacttat gtagaatata aatacttaag ttaggttttt ttgtgataaa gtttataagt
     1981 actcaatttt ataagataaa taatggtctt aagtgaaaag taaataagtt taaattttag
     2041 taagtattat attgcataag aggtgatatt attatgaaaa tagtaaccta caatattcat
     2101 aaaggtatgg attcaaataa tagattaact ctaactaaaa tggggttata tttaaaacaa
     2161 ttggattgtg atgtaatttg tttgcaagaa gtattatatc ctcaattttt agctttaaaa
     2221 gctgttttaa atatggatgg tgtctttgcg acaaatgtaa aaaaagtaaa tatgatatat
     2281 ggtatttgta catttacaac atctaaaatg ttaaacagta atcatttttt cttaactagc
     2341 aaaaaagaac aacggggagc tctttgtata actatagatg catatggaag aattataaat
     2401 gtaataaata ctcacttggg acttgatagg caggagagag caaaacaatt agacgaaata
     2461 atagactata ggaacagatt agtggggata gttattttat gtggtgattt taatgagaaa
     2521 aatgtctttt taagtatgtt taatgatatg gctatttcac taaacaagtc ttacttacca
     2581 acatttgaaa aatcaaactc tagaatagat tacatttttg ttaataaaaa tactgaacta
     2641 aaaggataca cagtagaaaa aatttacctg tcagaccatt atcctgtaat aggttacatt
     2701 taaatttcaa tataaatgaa attagttgtt ataaaataaa caattttttg ttaagaaata
     2761 gtaaaagaaa tataataaaa aatatcaaaa ataaaactaa ttataagaca taaatttaca
     2821 ataaagttac atttgattaa attccttgtt taacaaaagc tattcaaata taatctaagg
     2881 tagttttgaa atgggaactc tattatgcaa atttaagcta gtgttaaaat gaggtgaagt
     2941 tatgggcaga agaacaacaa gagtttcaag attaggtaaa gaaaaaaaac gaagtgtttt
     3001 aattgtattg gcagctttag ttctaataat gggtggattt acttattttt ttaacagtaa
     3061 atttctgtac aatgggaaaa tcgctaaaaa tgtatatata gaaggagtaa atgtatctga
     3121 tatgactaaa gcagaggcat taaaagctat aacagataag tatactccag aagatttaaa
     3181 tcttgcttat gatgggaaaa aatatacaat atcaccaaaa gatattgatt taaaatatga
     3241 tacagaagat gttgtaaaag atgcttatga aagtacaaaa aaagggtcct attttcaaaa
     3301 cttaaaaaaa tatattgata taagagtgaa taaggcaaat atgaagataa aagctgaata
     3361 tgatgaggca aaacttagtt ccaaagtgtc ttctatagca gatagtataa atgttaagat
     3421 gaaaaatgct tctattagtg ttggaagtgg tggactaagt tatacagact ctgttgtagg
     3481 tagagaattt gatttggcag caaataaaga gtctatatat aatatgataa aaaataaaga
     3541 gcataaaact ttagaactta aagtaaatct tcaaaaacca gatataacaa ctgaacaagt
     3601 aaaaactgta aattctgtta taggtcaata ttcaactact tattcacaag ctgtagaggg
     3661 taggtcatat aatgtaggat tatcagcaag aaaaactagt gatgtattat taatgccagg
     3721 tgaagaattt tcatacaata aacttacagg acctagtaat aaagcaaatg gttacaaaga
     3781 tgcacctgtt atagtttatg gtaagctaga acaatcagca ggaggaggtg tttgtcaaac
     3841 atcttcaaca gtttataatg cggctcttct ttcgggtatg gagataactc aagttacaaa
     3901 tcattcatct gcatctactt atgtgcctaa agggagagat gctactgtga gcgatggagg
     3961 attaaactta aagtttaaaa atccatataa gcatcctgta tatattaaaa attatgcagg
     4021 tggaggaagt gtatcatcag taatctatgg taattctggt gacaaaccaa atatatctat
     4081 agaggtaaaa caaactggtc agaataaata ttctacatat cgaatattta aagattcaag
     4141 tggaaaggtc ataaaaaaag aacatatatc aaatagttct tataaagaat taaaaaaata
     4201 gataattttt tagagaaata tttgtaatat aaaagggagt gtctcaaaat gaacttttta
     4261 gtttatgaga cactcttttt ctgtgaaatc aaatatattt tcatcatttc aacaatacaa
     4321 aagaagcgta tctctatttt gagacagcct tttttgtaaa aattaacact attagttctt
     4381 gttttaatat tacaatcatt aatatataga aaatattcct atatattggt ataattgtaa
     4441 atagatttag ttatataaaa tgtatgagga ggtgacatgg ttgaatttta taggcaatag
     4501 atatgaggta gtaaattctg acgacgtttt agaaattaac aaaatctata aagctagaga
     4561 tgttttttac aggaaaaatg tgttaataaa agttataaag cacaataatt atatctgtga
     4621 agattttgtt tcaaatttaa ttgatgaaag tactgcttta gatgagataa actctcctta
     4681 tatactaaaa ataatagatg ttgggattca ctgtacagaa gaaacaacat tatattatgt
     4741 tgtaagtgaa gactttaatg gtatcggact agatgaatta attctaggta attatcttca
     4801 tttagaagca attgtaaaca taatgataca agttttaaaa gctttagaaa tgatacatag
     4861 gaatcattca tatcatggta gtttaaaatc gagtagtata atagtagata cagaatacaa
     4921 tataaaaatt tgtgattttg gaattacaaa agcaaataat ggggttaata caagaagcta
     4981 tggaaacagg agatatttat gcccacatca actatgtata aattatactg ataaagaaag
     5041 tgattttttt gcaacaggtc ttattctatt tgaatctata tttaaaaaat tgccttttgg
     5101 agaatcaaat tcagaagaaa aaatgctaca atctattgat aagggattag attggaatag
     5161 tataaaagca attaatggaa atactgaact tataaatgtt ataaaaaggc ttcttggaag
     5221 aaataataag tatactagag ccaatgatat tattattgat ttaagtaaag taatgtatga
     5281 aaaagcatat atagaagttg aagatgagaa agaagaagag cagaaacaag acaaagttat
     5341 acaagttaaa aaagaatata aaagtaaaat gagaaaaaaa ttacataaaa aacttgttgt
     5401 tgccggtcta gctatagtca tgatttcaat gattattatt agttccataa tataaaaatt
     5461 gtatattaag tattttaaaa tcaatgtcta taaaggatat acatagaaat tttagtgtag
     5521 tcttaaatta tgaaaggaag tggttattat gttaacaaaa aataatgagg tattaaacgt
     5581 ctaggagtat tttaacaaat agccacctgc ttttacagtt tagaataaaa aagctgtggc
     5641 taaaggtcac agctttttgc tactttttaa aggcaggaga atattaaaaa ggagcaaatt
     5701 atgagcaata taaaaataat agcaagtaat aaatgttgga ttgaagatat agcaaggaaa
     5761 caacttgaag atatttcaaa gttaaatgga attttaagag tagtaggatt acctgactta
     5821 catgcaggta aaatacctgt aggtttagca gttgagacta aaaatattat ttatccacat
     5881 ataattggaa atgatatagg atgcggaatg actttattta aaactggggt tctaaaaaag
     5941 aaatttaaaa aagacaaatg gattaagtca cttagtaaga ttaaagattt gtctgatata
     6001 gaaataaaaa acacatataa agaagaatgt ccaattttaa atttaggaac tatagggagt
     6061 ggaaatcatt ttgcagaaat tcaatgtata agtgaaattt ataataaaga acaatttgaa
     6121 caattaaaat tttcatctga tgatattatg atgttagttc actgtgggtc aagaaattat
     6181 ggagaagata ttttaaaaaa attttatgat aaagatggac ttgaagttga aagtgagaaa
     6241 gctatagatt atattaaaaa acatgataat gctttaatgt gggcagagcg taatcgtgaa
     6301 gctatcagta aaaaaattat gcagtcaata ggtacatctg gagatgttga aacaatattc
     6361 tctataaatc ataattttat agagaaaagg gaagataaat ttatacatag aaaaggtgct
     6421 gtttcaagtg aaagaggagc tgtgattata ccaggttcaa gaggcagtct atcatatata
     6481 gttatgccta ctgaaaatac agagatatct ttatattctc tatcacatgg agcaggtaga
     6541 aaatggtctc gttcagtttg taaatctcgc cttaaaagta agtatagtaa agatactata
     6601 aaacaaacta aatttaaaag tcagattata tgtaatgatg taaatttatt atttcaagaa
     6661 gctccagagg catataagaa tatagagcag attattgaaa gtttgataga atatgaactt
     6721 attcaagtag tggcaactat gaagccattg attacttata aaggataatt tttaaagtta
     6781 aaaaagatta aaattatatt ataaatagct aattttatgg aacatttaaa agtgttttat
     6841 tattagctat ttttttattt tgtagagacg ataattt
//
LOCUS       DAELTA010000182         6853 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.183, whole genome shotgun sequence.
ACCESSION   DAELTA010000182 DAELTA010000000
VERSION     DAELTA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.183"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(137..766)
                     /locus_tag="KPG13_003200"
     CDS             complement(137..766)
                     /locus_tag="KPG13_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG0986891.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(845..1807)
                     /locus_tag="KPG13_003201"
     CDS             complement(845..1807)
                     /locus_tag="KPG13_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG0986892.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(2569..6084)
                     /gene="cbpA"
                     /locus_tag="KPG13_003202"
     CDS             complement(2569..6084)
                     /gene="cbpA"
                     /locus_tag="KPG13_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG0986893.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAGDAEGDTNAGDAEGDTNAGNAEGDTNAGDAEGD
                     TNAGNAEGDTNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEINVD
                     FKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTL
                     TPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVP
                     EGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIII
                     NGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGGGYYTT
                     TKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVT
                     GGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMN
                     KISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKGSEVSV
                     NLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEED
                     VEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTKQV
                     DAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKP
                     TDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTAS
                     IEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDIN
                     IDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDG
                     NPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDSG
                     NGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPD
                     PDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINID
                     TDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIM
                     LDLALMFISLFFLIKNLTNKYLRRK"
     gene            6353..>6853
                     /locus_tag="KPG13_003203"
     CDS             6353..>6853
                     /locus_tag="KPG13_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG0986894.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLI"
BASE COUNT         2126 a         1284 c          743 g         2700 t
ORIGIN      
        1 ttaaatttaa ataaaattta aattcaccaa aaagaaatct atattagaaa ccactactct
       61 attccaccag attgtaattt tttgttcttt atatcacaat gctataccat attaaattga
      121 aaaataagtt ttaaatttat ttgttacaat tcattaattc aaaaaaatat tttgagtcat
      181 gagttttgta aagatttagc catgaattta atgcttctga ttcaaaaaca tctaaaagtt
      241 tcaatacttg aaacgtaaat tccaacggag caattcctga agccgtaact aaattttcat
      301 cactaactac agattccgtt ttataatatt tttctccaga gtaatttggt attaccattt
      361 taagaaactc taagtcatta cttgtatgat ttctaaaatc taatagccct tttttagcta
      421 ggcctaatgt agctccacat atggctgcaa ctatagaatc ttgtcttaaa gcttctttaa
      481 ctttttctaa tattatctca tgattcatag ataaccaagt atttccccct ggcaaaatta
      541 ataaatcctt actttccata ttaaactcat caactgtaat ttcaggtaaa acttttaacc
      601 cacccattgt tataatagga tttttatcaa ttcctacagt aattactttt agtggcttta
      661 atcctttttt aaaatatctt cctgtattta attcagcagt taaatatgcc acttcccaat
      721 ctgacatagt atcaaataca tacatatata cttttctatt tttcatcttt tttactcctt
      781 atgtaattaa tttatatatc taattataaa ataacttaac tgacatattc agtcagttaa
      841 gctattaaat ttgataataa tcaattaatt ctagtaaatc ttcaattatt ttcttcctaa
      901 ggcttagtgg ttcaattact tgtattgtct ttctataagg taaaagtata tttggtatgt
      961 acttgcatat tacttcctct tcaagcataa atattatttt attaggtgtt ctttctttca
     1021 agtagtgccc taaaaaccaa tgttggctta aactatctaa agaatctata ctcccttcta
     1081 taactaattg aattatattt tgttcatctt ttatttcagg tataattcct tttataaaaa
     1141 attcacttgc tgaaaattct gtcggtctgt taaatgtatt tgatgtttct atgatttcac
     1201 taatcctatc cactctgaaa ctacgaacct catttctcat attacaaaat gcaactacgt
     1261 accaattatt attccagtga acaattccat atggatttat aattctatat tttgatttta
     1321 ctttacgacc tgcatgatag ttgatttcta cagatagctc ctttaatata ctattctcca
     1381 attttttaag tactgattct atagacactt tcccaattgg ttttattact tcaagggctc
     1441 ttaagtggtt tgttaaaata tcttcttgtt tttgatttga atacattttt aactttaata
     1501 tcgatttatc taatgcttcg tctaagaagt atccttcttt tttagcaaat agtgctgcat
     1561 gaagtagaga tttttgctct tctatatcaa aaaaaagagg tgctttagta aaattattta
     1621 aaaggctata cccaccatta tgacctgtat ctgaaattat aggtactcca ctcgcagaaa
     1681 gtacatcaat atatcggtac actgttctta tatttatttc taatttttca gctatctgtt
     1741 ttgctgtaat ttttttgtca gagtttaaca tccacagtat agacatcata ttgtcaattt
     1801 tagccatgta gtttcacctc tttctatttg ttatataaag ttattatata actttttgat
     1861 tgatttttta caaaaaaaca ttagtaaact gctaataatt tatactaaga tttatttcaa
     1921 atatttatag atagtctttt tctatagtta agttatccat taaattacat agtagtatat
     1981 ctattttagt tgatatattt tcagctacct cccttgaata ttattacaat aagtctaaat
     2041 cttctttctt ccataaatat attatgagga aagactatca tctttaatgg tttaatagta
     2101 taaaagatat taatttaaaa aaatctattt ttatatcaga tgggtattgt tttttataaa
     2161 actgaaaaaa tcacctaata gaatctatga ttttttaaca taacctccat aaattagttc
     2221 caattatcac ttcaaattca aatttttatc tgaagaatat acaatcatat ttccatactc
     2281 aacataatat tgagtgtttg gcttgtattt acaaattatt attctaacaa aaacaactat
     2341 acatagatta aaatcatctt ctaaacataa ctactaaaat tgaattaatg atatcaattc
     2401 cattaatctc ttttaataaa aaattctctt ttctatatga aaattatttc aaaactttta
     2461 agttaaatac aaaaatgact ataaatagat ttttatttac acaaatctat ttatagtcac
     2521 tatagtttat ccaataaaat aataaaataa atatgtacac aataaatgtt atttacgtct
     2581 taagtattta tttgttagat ttttaattaa aaaaaacaag ctaatgaaca ttaatgctaa
     2641 atctagcatt atattgcttt gatctcctgt ttgtacattt tgacctacat tactatctaa
     2701 actttcatct gtatctatat ttatatctgg ttttccattc ccatctgtat ctatatttat
     2761 atccggtttt ccatctccat ctatatctat atttatatct ggttttccat ctccatctgt
     2821 atctatattt atatctggtt ttccatctcc atctgtgtct atatttatat ctggttttcc
     2881 atctccatct gtgtctatat ttatattcgg ttttccatcc ccatctgtat ctatatctgg
     2941 tttgctattt cctccattat ctgggtctgg tttactattt ccaccgttat ctggatccgg
     3001 tttgctattt cctccattat ctggatctgg tttaccattg ccaccgttat ctggatctgg
     3061 tttgctattt cctccgttat ctggatccgg tttgctattt cctccattac ctgaatccgg
     3121 tttgctattt cctccattac ctgaatctgg tttgctattt cctccattac ctgaatctgg
     3181 tttgctattt cctccattat ctggatctgg cttatttaga tttggattac ctgtatcata
     3241 acctcctgag ccagtatcta catttttatc aggcttccaa taatcatcta ctccatcatt
     3301 atctgtatct attgctggac gattataatc tgtatcaggg tttccatctc cattggtatc
     3361 tatattttta tcaggtttcc aaataccatc tccatttcca ccttctgtac taggcttcca
     3421 ttcacctgta ttatcagtat ctatgttaat atctggcttc caatcaccac tcgtatctat
     3481 attgatatct ggccttccat caccatcagt gtctatattt atatccggta ttccatcccc
     3541 atctacatct acatttgtca tatctaactt taacttcttt tcatcctcac taccttcaat
     3601 gtaatttata tcatctccaa tatattttac agtaatagtt tgttcagtag taaaaatatg
     3661 atgattgcta tctaaaggtc tccattcaat agatgcagta ctataattat gactctcact
     3721 acccttatta tgagttttta attctactgg atctttatat tcctttccat taatatagaa
     3781 ttgtacgtaa cctcttggag aggcacaatc tggtccttct ttcttatctg gtgaaatagt
     3841 aacatttaat gtaaacgtat ctgttggttt aaaaacatca gttttagctt tagactcttc
     3901 tacaactaat gaagtttctg tatcagcagg aataatttct gccttataat atgctctatg
     3961 cccccaataa gcatccttaa aaggatatat actcgaaaac tgtgttgatg taatgcttaa
     4021 ttgatatttc ccagcatcaa cttgttttgt aagatcatca cttcctaaat ttatatctgg
     4081 ttccatcgtt tcctcattta aacgttgaga actaatagtc ataaattttt tatctgtgag
     4141 tttttcacct ttaggagtaa ttattggaat accaccatct ttaaatatcc aatctaaaaa
     4201 tgatttttct acgtcttctt caaatggtaa tccatcatat cttttaataa tcaaatatgg
     4261 tggagtatca tcaatagtaa attcttcata ttgctttaaa aattgagaac ctgaacctat
     4321 atctttaata taaaaatcat ctggttttag aacttctcca gtaattttat ctataacaac
     4381 taaattaaca gatacttcag atcccttaaa ttgatcagag aaccataagt aaagaatccc
     4441 ttgagcatct gtataactag gtgctccata aggataaatt tttccatcaa ttagcatttt
     4501 aaaatcatca actagtgtag tactaacttt atcttgccct aaatacgtta gagatatttt
     4561 attcataaaa acttttgttt tcttttctaa atcaccgtat gcaactgctc cacctaaact
     4621 tttgaatgtt gttgctttta atgaaccacc tgttactata acatctcccg ttgtagcact
     4681 taggtcaaaa atccaatctc ccactcctgt catataaggt aaaatcgttc ctccagttac
     4741 aagtatacga tatcctctat ttgttcctgg attaacacat ccatccccaa atgcactacc
     4801 atgtgcacct ccatatgaat aggaaagtcc accattaatt gtaatattcc cactataacc
     4861 agaacctgtg ctaggatcta ctactgaggg acttaatgca ttctttgttg ttgtatagta
     4921 tcctccacct attccagctc catggtatgc acctttagca gttataatac ctccattgat
     4981 tgtgatatct ccaccactac cattaccgcc tccacctatt ccagctccat atcctgtatt
     5041 acgactcgat tcttgaccta tagcagttat agcaccacca ttaataataa tatttcctcc
     5101 actttcagta tttccaccac caatacctgc agaataataa tttccaacaa cttttaaact
     5161 tcctctatta ttactatcta gtagtgtaag gcgatctcct ttttttaact tgtctccatt
     5221 ttctagagtt acatctctag gaatttttcc accctcagga actataaact ctccttcaat
     5281 atctctattt acaacagaat catcaataat tagagtagta ccttttcctg ctctcatacc
     5341 tggataatat acatcacctg actctagctt gttttcacta ccatcttgta atataacatg
     5401 tgcatatgca ccaccatctt tatctggtgt taaagtaaat gggctattag atgcagtttt
     5461 aatatttact ccatcaaata ttaattcagc ttttacatct ttaggtataa caatcctatg
     5521 tgttgttgga gtatttggat tgttattttg aattgtcatt tgttttccac taagaatagt
     5581 taaaacgcca cttgcaaatt taaaatcgac atttatttct ccaccactaa ttttaaagtc
     5641 tcctacagac gtaggctctt ctgcagtggc ttcttcttct acaactactg cattagcatc
     5701 tccttctgca tctcctgcat tagtatctcc ttctgcatct cctgcattag tatctccttc
     5761 tgcatttcct gcattagtat ctccttctgc atctcctgca ttagtatctc cttctgcatt
     5821 tcctgcatta gtatctcctt ctgcatctcc tgcattagta tctccttctg catctcctgc
     5881 attagtatct ccttctgcat ctcctgcatt agtatctcct tctgcatttc ctgcattagt
     5941 acctccttct gcatttcctg cattagtact ttcttctata gtatctgcga aagtatttga
     6001 cgcattaaat gatgctactg gtccaagtcc cattgagata cttaaaatca ttgatagtgc
     6061 tttcttttta catttttgtt gcataaactc cccctcccct taataatttt taatatacac
     6121 tgtatacatc cttatataca ctctaataat tcttaatata cgctgtatac attctaatgc
     6181 acactctaat aatttttaat atatacttaa aacttatatt tgtcaatgaa tatactgttt
     6241 tttcaacctt atatattata agtattcatt atttgctaaa taactacaat aaaaatttta
     6301 attatataaa atttatttaa ttttttatat aataatacta aggggtaatt aattgaaaaa
     6361 actagtaaaa atattaataa atattatttg cattattacg ctaattttct ctagcctttc
     6421 gatttacata aaattatctg aatataaaaa agctgatgaa gtatatacgg aactcagaga
     6481 aaacactatc aataatagca aacatcaaga actttatgat aaaaataatg attatagatt
     6541 ctggcttaaa ataaacaacg taaatataga ctatcctgta gtacaaggat ataacaatga
     6601 tttctaccta actcatgatt tttataaaaa ttatcttcct ttaggtagta ttttcatgga
     6661 ttatagaaat aattttgaaa atgataagag tttaatagtc tatggtcatt atatgaaaaa
     6721 taaaactatg tttggtcagc tagaaaacta tactgatgaa gcatttttca aagaaaataa
     6781 cttagtagaa ataaattata aagtacaaac ttatacttat gaaatttttt cagtatatac
     6841 tgctgactta att
//
LOCUS       DAELTA010000183         6791 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.184, whole genome shotgun sequence.
ACCESSION   DAELTA010000183 DAELTA010000000
VERSION     DAELTA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.184"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            195..1046
                     /locus_tag="KPG13_003204"
     CDS             195..1046
                     /locus_tag="KPG13_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0986895.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1050..1520
                     /locus_tag="KPG13_003205"
     CDS             1050..1520
                     /locus_tag="KPG13_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986896.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1533..2843
                     /locus_tag="KPG13_003206"
     CDS             1533..2843
                     /locus_tag="KPG13_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0986897.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3055..3801
                     /locus_tag="KPG13_003207"
     CDS             3055..3801
                     /locus_tag="KPG13_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG0986898.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4163..5704
                     /locus_tag="KPG13_003208"
     CDS             4163..5704
                     /locus_tag="KPG13_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG0986899.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5739..>6791
                     /locus_tag="KPG13_003209"
     CDS             5739..>6791
                     /locus_tag="KPG13_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG0986900.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVN"
BASE COUNT         2397 a          908 c         1196 g         2290 t
ORIGIN      
        1 atgtaatatt ttagatattt ttcgttgaca atgttaaatt tttgttttat ccttaattaa
       61 ggacatgtta ttgacatttc tgaaacttat ttgaaatagt tttttcattt tatttaggaa
      121 aacgtattat acttaagaaa acgtattttc tcatttcaga taagtaaaat atttattctt
      181 ttggggagga aatcatggaa attaataaaa aacattttat tcaagtattt ttaacttgct
      241 taatcttggt agtaatatgt gaatttatag ggcaaagaac tatagctatt ggaaaagcct
      301 ctatagtact gttccctatg ctttatgcag ttataattgg acttataata acaccagatt
      361 tattaggtaa aaaaattaaa gcacttaaaa aagtggttgg agaaaaagaa ataaatattg
      421 ctggtgaagt tgttggaatt gcactagtaa tgcttggtat aaaatatggt actacagttg
      481 gaccaaacat tgacaaaata attcaagctg gaccagcgtt tatcgctcaa gaatttggtc
      541 atatacttgc accaatcgtt gcattacctc ttgcattaat gtttggaatg aaaagagaag
      601 ctgttggagc tactgcaagt ataagtcgtg aaccttcttt aggagttata tcagaaaaat
      661 atgggataaa ctcaccagaa ggtagtggtg tacttggtac atatttaatg ggaacagttc
      721 ttggtactat atactttagt gtactaggtt caatatctat ttatagtggt cttcacccat
      781 atgctttggg aatggcctgt ggtgttggta gtggaagtat gatgacagca gctgctggtt
      841 cattatctac catggtacca cctgaaatgg cagatacaat acttgcctat gctgcaacaa
      901 gcaatatgat gtctagtata acaggaatta tattcttagt ttttgtatcc ttaccattta
      961 ctaattttat gtataagatt ttagaaccta aattttcgag aaataaaaaa actaaagaaa
     1021 ttaaatctac agaaggagag tgctaataaa tgtttgctaa tttaaattta aagaaaagtt
     1081 catatcaatt attggttatg tctgttattg gagttataat tcttatagga caaagaatta
     1141 gcgttggtac atctatagtt acagctcttc ctggtatggt gatgctaata ttagctgcta
     1201 tggcagctat gattataaaa gatttatttc ctaaatcaat ctttccagct tttggatttg
     1261 caactataat cggcctttta cttagtatgc catatagtcc aacatctgaa gtattcttaa
     1321 ctaacactaa taatattaac tttatggcta taactactcc actactagct tttgcaggaa
     1381 tttctgttgg aaacaaaata gaagctttaa aagaaatgtc atggaaaata gttataattt
     1441 cacttattgt atttacaact atattctttg catgtgcgag tatagctcat atagttttaa
     1501 gtattcaagg taaaatatag gaggaattta ttatgaataa agaacaatta aaatctctag
     1561 taataaaaac tatagatgaa aatagagaaa aaatattaaa aataggtcaa ggaatataca
     1621 aaaatccaga atatggatat aaagaattta aaactacaga agctgtttca aacttttttg
     1681 agcaagaatt aaatttatct gtagaaaaaa atatagcagt tacaggatgc aaagcagatg
     1741 caaattctag caagaaaggt cctcatatat ctatacttgg agaattggat ggtatatcat
     1801 gtaaagaaca taaagatgct aatgaaatag gtgcttctca tacttgtgga cataatattc
     1861 aaatagctgg aatgttaggt gctgctgttg gtttagtaaa aagtggcgtt ttagaacatt
     1921 tagatggtaa agtatctttt atggctactc ctgctgaaga gtttattgag ttggaataca
     1981 gagagcaatt aaaaaaagaa ggaaaaataa cttattttgg tggaaaacaa gaattaataa
     2041 aaagaggtta ttttgatgat atagacatat ctatgatgtt tcatgcttct gatttaggag
     2101 aagataaggc tctagttggt cctgagagta atggatttgt tggaaaaaag gttaaattta
     2161 taggtaaaga atctcatgct ggttctgctc cacatgatgg aataaatgct ttaaatgcag
     2221 caatgcttgc tataaacaac gtgaatgcac ttagagaaac ttttaaagag tctgaaagag
     2281 ttagattcca ccctatcatc acaaaaggtg gagatatagt aaatgttgtt cctgctgatg
     2341 ttcatatgga atcatatgtt agagctagaa ctataaatgg tatgattgat gctaatgaaa
     2401 gaataaataa ggctttgatg gctggtggaa tggcagttgg agctgatgtt gagataactg
     2461 aaattcctgg atatcttcca atattacgat acaaaagtct tgataattta tttaaaaaca
     2521 atcttgaaga tttaggtcta aaaggaaaag ttattgatgg tggagatttt acaggctctt
     2581 ttgattttgg agatgtgtct catataatgc ctactcttca ccctatgatt ggtggtgtaa
     2641 aaggagctct tcatacaaga gattttgaaa tagttgatga agatttagct tacataattc
     2701 ctgctaaagc aatggcactt acagttgtag accttctttt tgataatgct aaggatgcta
     2761 aagatatatt agataccttt actccagtta tgactaaaga agaatatcta acattcttag
     2821 aaaaacacga taaaacttta taagtctatt tttataatag ggtggtatct actgtaaaag
     2881 ttataccact ctttgttttt gtaacagtat acttcttttc ttttagtttt tagattaaac
     2941 taaaatatat tatcctcctt aaaactttta gagctacgtt gaaatatcag tagaactatg
     3001 ctatattctt tctaattaat ataaatttct attctaattc aaggtggtga aaaaatgttt
     3061 aatgcaggaa atcgaattat aaatacatgg ttgtacccta ttcaagatgg ctttgtcttg
     3121 attgatactg gatatgaaaa tggttttaaa catttcaaaa aacgaatttc taaattcaac
     3181 ataaaaatca aagatattcg atacatattt ctaactcatg ctcatgatga ccatgttggt
     3241 tttctaaatc agattctaga cgaatctcct aatacaaaga ttgttatgca tgataaagct
     3301 ttagaaattc tatataaagg tcaaaactct tttattggtg gttgtaccag ccgtactgcc
     3361 tttctctttt gccaaattat gaaactattt ggaaaaggtg agcatagttt ttctccactt
     3421 aaacaagaat ttgaaaaaag gtgtattctt gtgtctgaca agaatcgtaa agaaattgaa
     3481 aaagaactaa atggaaaaat tatagataca ccaggacata ctgatgattc aatttcacta
     3541 ctaattgatg aaggtgtttt attttgtgga gattctgcaa tgaatggatt tccaagtact
     3601 cacaaaatta ccatatttgc agaaagcaaa ctagaattta ttcaatcttg gaaaacaatc
     3661 atcaatacaa aacctaaaat gatttatcct ggacatggaa aaccatttga atacagtaaa
     3721 ttagaacaaa atctaatcta tgtaaataaa ataaatttat atcctctaca taattcaaaa
     3781 gatattcaca gatttttgta gaaaatacaa agtaaattta tttgattaat taaaaaatct
     3841 aacactttac tacgctactt attttaataa cttaatcttc cactagcttt tcataaaatt
     3901 ttgttttaaa attaatttta aagtgcatat gttatttaaa tacattagat aaaattcgtc
     3961 attaatctac ttaaagcata tttaactaac accactcaac cagaaaattc agaaaactgc
     4021 attgacacag gaaaaagttt attttatact ttttttagga catattgtgg acacgtctta
     4081 aatcgacaaa cagaatttaa attgtaagat taaccacaat acttattttt taattatatt
     4141 tttaaacatt agggaggata ttatgaatga taacgtagtt aagaagaaaa acttttttga
     4201 caggacatta aacagaatag aaactgttgg taataaacta ccagacccag ttacaatatt
     4261 tttagggtta tgtgtacttt tacttatact atctagtcta gttggttcta tgggtatatc
     4321 agtagttcat cctggtactg gagaaactat aaccgctgta aatttattga ctgtagaaca
     4381 gcttcaaatt cttttaggaa acatagtcag caatttccaa gggtttgctc ctttgggtct
     4441 agttctagta actatgatag gtgctggtgt atgtgacaaa acaggactta tgacagcaac
     4501 aatcaaagca tcagtaagca agattcctga aacaagagta actcttgttg taatgactat
     4561 aggtatgctt gccaatatag cttcagatgc aggtacaata ttatttccac cattagctgc
     4621 acttgtatat cttggtgttg gtagacatcc acttataggt ttattttctg gatatgcagc
     4681 agtctgtcta ggatttgccg caaacattat gataagtgtc aatgatatac ttgctgcaag
     4741 ttttacagtt ccagcagctc aaatgattga ttctggattt caagccaatg caacaatgaa
     4801 cttatttttt atgatagcat caacttttgt tctaatactt ttggcaacat gggtaacaga
     4861 aaaaattgta gctcctagat ttggtaaata tgaaggaaat gcagaactag atgttgatgg
     4921 aagtcttagt gatgtagaaa agaaagggct taaaaaagct agtataagta tattaatata
     4981 tgtggctatt atagtagctc ttagtgtaat cggtaaaaaa ccattcttag ctgacccaga
     5041 aacaggagat ttactatcaa acaatgctcc tcttatgaaa ggtatggtac ctataatagc
     5101 actagcattt ttcatacctg gtgttgtata cggaaaaact ataggtaaaa taaagaagga
     5161 taaggatgta gtctctataa tggctagtgc aatgagtgat atgggtggat atataatact
     5221 agcatttgct gcttcacaat ttttgcaatt attcacaaat agtaatttag gtctgatact
     5281 agctgtaaag ggcggagaat ttttaaaatc agcaggaata aatggacctt tattgttgat
     5341 aggatttatt atattatcat gttttataaa tttatttata ggtagtgcat ctgcaaagtg
     5401 ggctattctt gcacctatat ttgtaccaat gtttatgatg gttggataca atccagcact
     5461 tactcagatg gcttatcgta taggtgattg tataacaaat ccacttagcc cattatttcc
     5521 gtacttccct attatactag cattcacaag aaaatacgat aaagatgctg gtatgggtac
     5581 tgtaattgct aatatgatac catactctat atcattctta atagtgtgga ctatactatt
     5641 aatattgttt atggtattta atattccact aggacctgga atacttcctt catatagcat
     5701 gtaatttatt taaatctttc taaaaggaga aataatttat gttagataaa aatagactta
     5761 taaataattt tatggatatg gttagaattg atagcccatc taatcaagaa ttagaaatgt
     5821 ctaagtggtt ggtaaactac ttaaaagaaa gaaatataga tgccattata gatgatgcag
     5881 gtgaaaaata tggtggtaat acaggtaatg tcattgccta tataaaaggt gaagaaggaa
     5941 gtagaccact ttgtttatgt gcacacatgg accaagttca accttgttta ggtgtaaaac
     6001 caatactaga tggtaatgta gttagaagtg atggaactac tacacttggt gcagatgata
     6061 aagctggaat agctgctata cttgaagctt tagaacatgt tattacagaa aaaataccac
     6121 atagagatat ttatctttgt tttacaatct gtgaggaagc tggtatgcat ggtgtcaaaa
     6181 actttaatcc tgacaacttg ccttgtaaag atatggtgat tttagattct ggtggagcta
     6241 ttggttcgat agcatacaaa gctcctgctc aacaatctat aaaaatctct tttcaaggaa
     6301 agaaagcaca tgctggtata gaacctgaaa aagggttaaa tgctatatta gtagcctctc
     6361 atgcaataag caacatgcac ataggcagaa tagattcatt gactacttca aatataggtg
     6421 aaatagaagg tggtggagct acaaacatag taactgataa ggttacattg actgctgaaa
     6481 ttaggtctca tatacctgaa actttagagt atgagcttaa tcatatggaa aaatgttgta
     6541 aggacgctgc atctaaattt aatactactt atacttttga acataatatg tcttacccta
     6601 gttttgaact tagtagagat agtcatgttt tcaagttaag tgaagaagca ataagacaag
     6661 ttggtgttgt tccaaatcca atggtaattg gtggtggaag tgacgcaaat atattagcta
     6721 atcttggata taattgtgct atattaagtc taggtatgta tgatgtacac acagttaatg
     6781 aatatgtaaa t
//
LOCUS       DAELTA010000184         6765 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.185, whole genome shotgun sequence.
ACCESSION   DAELTA010000184 DAELTA010000000
VERSION     DAELTA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.185"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..271
                     /locus_tag="KPG13_003210"
     CDS             131..271
                     /locus_tag="KPG13_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986901.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(268..639)
                     /locus_tag="KPG13_003211"
     CDS             complement(268..639)
                     /locus_tag="KPG13_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986902.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1188..1445
                     /locus_tag="KPG13_003212"
     CDS             1188..1445
                     /locus_tag="KPG13_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986903.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1555..2235)
                     /locus_tag="KPG13_003213"
     CDS             complement(1555..2235)
                     /locus_tag="KPG13_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0986904.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2495..2794
                     /locus_tag="KPG13_003214"
     CDS             2495..2794
                     /locus_tag="KPG13_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986905.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            3118..4773
                     /gene="ilvD"
                     /locus_tag="KPG13_003215"
     CDS             3118..4773
                     /gene="ilvD"
                     /locus_tag="KPG13_003215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG0986906.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            5117..5746
                     /locus_tag="KPG13_003216"
     CDS             5117..5746
                     /locus_tag="KPG13_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0986907.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            5772..6584
                     /locus_tag="KPG13_003217"
     CDS             5772..6584
                     /locus_tag="KPG13_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0986908.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         2667 a          752 c         1068 g         2278 t
ORIGIN      
        1 atctagggaa attttggata atcaaaatat attttataaa atcaaaatac aattacataa
       61 actttattaa aaatttgttg ggaatttctg actaacatta agaacgtaag tttgttgggg
      121 gatggtatat gtggaaaagt caaacactac tgttacaaaa aaagaaaaaa ttgaagatat
      181 aggaatgaaa ttagaacatt tattgagtga agatattact ttttatgaat tattacttga
      241 gattttgtat aaagaaaaag aagagaatta attctcttct ttaaggaatc tttttaaata
      301 ggcattatca atcatttttt ctatagcttc tctatcttct ttttcaagag aatatatttt
      361 ttctaagagc ttttttgttt tttcacttac tgataagttc tctatgatta atttatcttc
      421 tttgcttatt tttgtattat catttacgcc taataaataa tctgttgatg tttctaatgc
      481 ttttgctaat ctaactatta tttcactttt aggttctctt aaattatttt cataacgtga
      541 caagctagcc tcagttatac cgactttagt agcaagttct ttttgattta tatctaattc
      601 ttttcgtaga tttgctattc tatctcctaa actttccata tgtacatcac tccttaccga
      661 aattataagt tattttgata aaaagtaaaa taaaattacc aaaaatataa aaaaaaaagg
      721 ttaaaataat aaaatatatt gaaaattacc atttgtatat gtataatatg aattatacaa
      781 atggtaattt gatgagaggt ggtatgttga aataagaaac tgaattaatt aataaataca
      841 tagaaagttt tgaaaacctt taattgagat ataaacaata tgtacaaaat tttttgcaag
      901 taaaattacc aaatgtataa aataaatcag tagcatagat aattttatgt tttaatacat
      961 tagattattt aatttagtta taatggtcaa aaatgcaatc tacaaataaa acaaagtatt
     1021 atcaaaaaag tattctttaa gaaaattttt tattagagat gtaattgata taaataatcg
     1081 tattaaaaaa taaaatacta agtttaaaaa tttaaataat ctataaatca attataaaat
     1141 attatttatt agaaattaaa atgaattaag taaatggagg aattgatatg ataataatta
     1201 gaagtcaaga taaaactaat ttaatgcaga taaaccaaat taaaatagat ggtagtcaag
     1261 tttatgcagt atttgaaagt aaaattgata ctgtaaaaat aggagattat gaaaataata
     1321 caagagctat acaagtgttg gacaatatac aaaacttcat tgaaaatgga acaaaatatg
     1381 attatataac atcaaataaa gtcagataca atgttaataa aatatttcag atgccagcta
     1441 aatagtgagt gtgcatttta atttaataac taaagcccac tttcttttgt agtatgaaag
     1501 agggctttac atatttgtaa atatcgaagt acacttattt tatcaataaa ttatttaata
     1561 aggtataatg gaccaattaa acaataaaaa ttctcctaaa acagcaagta ttaaatatat
     1621 aatagcttca tttttgtaaa gagaggtaat ttttttgctt atgtcatcat caattgtaat
     1681 agcaatctca tcttgaataa tatttttata tatagatggg aaaatatctt ttttctttat
     1741 aagattaaat aaattattta cacctaccca tataaaatat cctaaaacag cacctaatgt
     1801 gttcatcatt aaatcatcaa tatctgtagc tcttaagtta aacaattgac aaagttcaat
     1861 tgctaatgaa aaacctaatc cagttaatga aacattgata atatttctaa aatttttcca
     1921 aattaaaggt agtaaaaatc ctaaaggcat aaacataatt atatttaaaa tatatgttaa
     1981 gatgccaact gagtcaaaag gtattagatt tatttcatca attctaaaaa gcccactata
     2041 ccttcctata tcccatatgc taccaattcc agctttacta agtgctagat agatatataa
     2101 taaaaaaata tatacccata ctaaatgtat cattacattc ttttcaactt tcaaattttt
     2161 tcgttttata acgactattt ggtatattaa gcatggtaat ataacgcaaa taaatgaata
     2221 aaatgtccat atcattttat tttgtcccct ttcaaattta tgtttttagt tttataagta
     2281 ctatctttat attaaactgt aagttttaaa aatcttttaa taaaatctta aaaatttatt
     2341 aatttataaa aatatattat taagattaaa aaatatatat aatcaaagtt tctacaatta
     2401 tatagttagg gatataaaaa ttttatcgta tgcataaata ttataaatat atggaaataa
     2461 taatagtata ttttggtaaa gtaggaggaa ttttatgaaa tggattaaat ggtatagtat
     2521 tacatgcata tgtattttta tagtagtagc tttttatatg tttatatttc ctaataaaat
     2581 agaaactatt gatacttcat ctgcatatag ctttgttgaa aagaaagtac caaatagtgc
     2641 tgtgtatcaa ggatacaaaa agaatccagt tgatggtact actacaattt attacagcta
     2701 tgataattca actcatatag ttagattatc acatccagaa gactattcaa ggaagattaa
     2761 ttgggataaa gtttcaaaca tcagatttga ttaatcttta atatttataa aagtctatat
     2821 atctaaggca aatatattat taatcttatt catagttgag cactttctat tatagaagtg
     2881 cctcttctga tttatatcat aaaatattac tcaacaattt gtaaaatata ttagtttata
     2941 aaatatattg aaaagataat attatataat acgattgtgt aagatattga taaaaaatcg
     3001 taataaattt tcattagtat atgctaatat ttttttacaa ttttcgtaat taattaatag
     3061 aaaaatatat tataataata ttatgaaaat ttaaaaaagt aagaaagagg taatattatg
     3121 agaagtgata taaaaaaagg aatagaagga gctccaaaga gagcgttaat gtatggaatg
     3181 ggtcttacta aagaagaaat tgaaagacca ttaataggta tagtaaatgc acaaaatgag
     3241 gtgattcctg gtcatctgca tcttgatgaa atagcagaag ctgcaaaaaa tggagtaaga
     3301 atgagtggtg gtttgccatt agaatttcct gctatagggg tatgtgatgg tatagctatg
     3361 gggcatgtcg gaatgaatta ttccctggct tcaagagaat taatagcaga ttcaatagaa
     3421 gctatggcta tggcacatgg ttttgatgct ttggtactta tacctaattg tgacaagata
     3481 gtgccaggta tgttaatggc tgctgcaaga cttaatatac caagtattgt tgtgagtgga
     3541 gggcctatgc ttccaggtaa aaaaaatgga aaagtatatg attttaactc tgcaatggag
     3601 ggtgttggag catgtaaaga tggaacagta agtgaagaag aattagaaga gttagccatg
     3661 aactcttgtc ctggatgtgg ttcatgttct ggtcttttca cagcaaatag catgaattgt
     3721 ttaacagaag ctttaggcat gggtatacca tataatggta ctgctgcgtc acattctgga
     3781 gaaagaaaaa ggatagcaaa atatgcaggt atgtatgtta tggagttact taagaacgac
     3841 ataaaaccta gagatatttt aacaatagat gcttttaaaa atgctatagc tgtggatatg
     3901 gcaatggctg gttctacaaa tacagtactt cacttacctg caatagctta tgaatcagga
     3961 atagagctta acttagattt ttttgatgaa ataagtgaaa aaactccttg tttaacaaaa
     4021 ttaagtccaa gtggaaaaca tcatattgaa gatttacata tggcaggagg aataccagct
     4081 ataatgaacg aactttcaaa gataaatgga ataaatttag attgcaaaac cgtaacaggc
     4141 aagactatag gggaaaatat aagaaattgt gaaatagaaa atgaagaagt aatacataca
     4201 ttaaagaatc catatagtaa ccaaggtggg cttgcaatat tgaaaggaaa tcttgctcta
     4261 aatggagctg ttgtaaaaaa gtcagcagtt gcagaagaaa tgttagttca tgaaggacca
     4321 gcaagagttt ttaattcaga agaagaagct gtaaatgcta tttttggtaa aaaaataaac
     4381 aaaggtgatg ttatagttat aagatatgaa ggtccaaagg gtggtccagg aatgaaagaa
     4441 atgctatctc ctacatcagc agttgcagga atgggacttg ataagcatgt agcacttctt
     4501 actgatggtc gtttttcagg ggcaactaga ggggcatcca taggccatat ttctccagaa
     4561 gctatggaag gtggtttaat cggactagtt gaagaaggag atataatttc tataaacata
     4621 ccagacaaaa aattagagtt aaaagtagat gaagttgaaa tagaaaatag aaaattaaaa
     4681 tttaaacctt tagaaccgaa aataaagcat ggatacttaa gtagatatgc taaactggta
     4741 acatcagcaa atacaggagc agttttaaaa tagtagacta tatttatgta aattagaaaa
     4801 agttaagaaa atattaattg gatatcttag gtgttatagc actaatgagg tatccttttt
     4861 attttgtatg aaaaacatag caaaatcttg attatctttg aaaacaatac atctctatta
     4921 taaggaatac gagtacagca tagagatgta tcttttagac cttgttgagg taatacaact
     4981 ttttaaaagt ttgatgtgga atgcttttcg ttccttctga actatgaaga aattgaaagt
     5041 aagttaattc ttgacataat aaatattaaa atgtataata aaaaacaaac agacagtctg
     5101 tttttaggag ggaaatatga atagagaaga aaaaagtaaa aatagtaaag aaaaaattat
     5161 ccaatcagca ttttcactat tttcgtctaa aggatatgat tcaacatcta cacaagatat
     5221 tatcaattta tctggtctat ctagaggtgc aatgtatcat cactttaaaa ctaaagaaga
     5281 tatactgaga agtgtcacaa aagaacttta ctcacaaatg aataattttt tagagcatct
     5341 tgttgctgat gacaccctta cagcaaatga aaaaataata gaattggttg ttcatagtgc
     5401 gaatgattac acacgtagaa aaatggtaca ttgtagctgg ttagaaaaaa tcccattcgc
     5461 tttaatagag gaagttcgta atctcaacaa tgtagttgca cccaatattg ctaagataat
     5521 taaacaaggt gttgaaaata aagaattttc ttgtgaatat ccagaggaat tagctgaaat
     5581 gctcgttttt agtattgata ttttacttga ccctgtatta tttaaacgtg aatacagtga
     5641 agtatgtaat aggttggatt ttttattatt catgttaaaa aaaatggata ttcctctaat
     5701 tgatgagtat ggtattcaaa aatttaaaga tttatttaaa caatgataat aggaaaagtg
     5761 aggaatagac tatgttttat aatgcaaaaa gttgtcaaat gatgatagga agtaatacag
     5821 tgaattatat tgaatttggt agtggtaaga agacactaat tattttacca ggattaggag
     5881 atggtttatt tcctttacat ggaaaaatac aggcgattgc ttttgctttt agatataagc
     5941 aatttacaaa agattataag gtttatgtat ttagtagaaa aaatcaaatt acagaaaaat
     6001 attccacaag ggatatggct aaagaccaag cagatataat gaaaaaactt ggaattatga
     6061 aagcagaagt aatgggtgtt tctcaaggtg gaatgatagc tcaatatctg gcaattgatt
     6121 atcctgagtt agttgagaaa cttgtgttgg cagttacttc ttcaaagcaa aatgatacta
     6181 tccaaaatgt tatttgtagt tggattgata tggcgaagaa gcagaattac aatgatttaa
     6241 tgattgacac agcaaagaaa tcctattctg aaagatattt aaaaaaatac caattattta
     6301 ttcctttttt aggaaaagta ggtaaaccaa aggattttaa acgatttatt attcaagcga
     6361 cctcttgcat agaacataat gccttttccg aattaaataa aataacatgt ccaaccttaa
     6421 ttataggagg tgctaatgat aaaattgttg gaaataatgc ttcttttcat ttagtagaaa
     6481 aaattaaaaa aagcgaaata tttatctatg agggattggg acacgctaca tatgaagaag
     6541 cacaggattt taatgaaaga gttttagagt ttttaaataa gtaaacacct ttgttaaaaa
     6601 tatacatatg aagattggaa atttaatgtt aaaaatagaa acatgaaaat atgcttaact
     6661 ggtattttta ctattcataa ccaatttttg atacattatc tactataaat acaaatatag
     6721 cttcaatgtg attatatatt gttgtattgg taaagcactt ataca
//
LOCUS       DAELTA010000185         6709 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.186, whole genome shotgun sequence.
ACCESSION   DAELTA010000185 DAELTA010000000
VERSION     DAELTA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.186"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="KPG13_003218"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="KPG13_003218"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG0986909.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="KPG13_003219"
     CDS             complement(2851..3216)
                     /locus_tag="KPG13_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0986910.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="KPG13_003220"
     CDS             3415..4263
                     /locus_tag="KPG13_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986911.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="KPG13_003221"
     CDS             complement(4700..5788)
                     /locus_tag="KPG13_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986912.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="KPG13_003222"
     CDS             5913..6641
                     /locus_tag="KPG13_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG0986913.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2353 a         1114 c          725 g         2517 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgac
//
LOCUS       DAELTA010000186         6660 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.187, whole genome shotgun sequence.
ACCESSION   DAELTA010000186 DAELTA010000000
VERSION     DAELTA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.187"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(547..954)
                     /locus_tag="KPG13_003223"
     CDS             complement(547..954)
                     /locus_tag="KPG13_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986914.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1428..2312)
                     /locus_tag="KPG13_003224"
     CDS             complement(1428..2312)
                     /locus_tag="KPG13_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0986915.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2603..3079)
                     /locus_tag="KPG13_003225"
     CDS             complement(2603..3079)
                     /locus_tag="KPG13_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG0986916.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3345..4520
                     /locus_tag="KPG13_003226"
     CDS             3345..4520
                     /locus_tag="KPG13_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG0986917.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4601..5404)
                     /gene="proC"
                     /locus_tag="KPG13_003227"
     CDS             complement(4601..5404)
                     /gene="proC"
                     /locus_tag="KPG13_003227"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG0986918.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5444..6028)
                     /locus_tag="KPG13_003228"
     CDS             complement(5444..6028)
                     /locus_tag="KPG13_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986919.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2197 a         1125 c          743 g         2595 t
ORIGIN      
        1 ataataatgt taacaaatgt aattatttaa aacaattatt tagaaggttt tatatttaat
       61 tttaaataac atatttaaga accgtaagtc ttaactttta tcatttttga ggttgtcgtt
      121 cacataagtt cgctactacc tatgcagttc tcttatgaac ttcttacact ttcatgtaag
      181 cacagactat atcttatcct tcagcattat ccgttaaggt ctacccactt ccactaccaa
      241 tagcttgtag tgtacttccc tcaagaggaa tagtcgttga agtttctcct attcggagtt
      301 tacctgctga ttgcccattt taaagtactt aggatttaac cttataccat ctaaccaatt
      361 ttttctactt tcgtaacttt cacacttagg tttatttcat ccttatgttt tagtttgatt
      421 agctttaggg gtttccagca attcaagtag tattggatag gttctatcct atctctacat
      481 ataggtttcc ctatatgctg actattttag ctactaactc atgactaaag tcacgagtgt
      541 gcgtagctat tttttaatca atattttagt aaattttacg gtaagtaaaa acctctctcc
      601 ttcaaatcct atagcaaata tacttacaag ttgctttgag aaaataactc ctaatatagc
      661 tacaactatg catataccaa ctattatatt taaaacattg tttaaaaact ttagtgcttg
      721 cttttcacct tgtttactac ttatatcttg atacattgga ataaaagtag taactatagc
      781 agtacctatt gctgcaaata taatatttgg tatattcata gctgttaaat aactctctgt
      841 atataagcct gtaccataaa ttgatgataa tactaattct ctacccattc caagaatctt
      901 agagattata gtaactatca tcaaataaaa agttgcttta gctaccttgc tcattttatc
      961 actccacttc taacatacct ctttttaatc aagtaaaatt tagctcatta gatataattc
     1021 tatcaactaa tttgccttgc ttagccctat caaaatataa ttaacaccta tttcttaata
     1081 tatcacaaac tacattattc ttcatatttt tttatttctc caataagttt tacatttttc
     1141 ctatataaat tgtatagctt caattatatt caaagaaatg gagttgctac ccgataactt
     1201 taaaagtaaa aaacagattt tattatcttt ttaaactata ttctaacata ttttgtccat
     1261 attgagaaat tttactaaac tattatgtac tttattaaat aaataaaata acagcacacc
     1321 tataaaatac aagtgtgctg ttcataaaca gattttataa aaaaataatt cctaagtaga
     1381 gattaacatg ctttctcata ctctcatata taatgtgtct aaaaatatta ataaaaatgc
     1441 tttaattgtt ttaacttact tggatgtctt aattttctta ttgctttagc ttctatctgt
     1501 cttattcgtt ctctggtaac tccaaatacc tttccaatct gttctagtgt tttagggtca
     1561 tcatcatcaa ttccaaatct cattcttaaa acttgctgtt cttgctctcc aagacctgta
     1621 agtagttctt ctatcttttc ttttaaatta tgctgttcaa cttcgtgtat tacaacatct
     1681 ttaaaatcag catctgatgg tataaattca actaaactac tatcctcatc ttcttttaat
     1741 ggagtatcca aagatacaac atttttatct cttcttaata attccaacac tttgtcaact
     1801 tctaagccac atgcatctgc aatctcatct atagttggct ctcttaataa tacatttaaa
     1861 agttcttgtt tctttttctt tacaaaattt attctctgat gaagatgtat aggtatccta
     1921 atagtatttt cgcaatcgtc gatatatctt gttatacttt gttttatcca ccaagttgca
     1981 taagtgctaa atttgtatcc ttttttataa tcgtactttt ctacagcttt tataagtcct
     2041 atatttcctg cttgaattaa atccaacata tcaatactat ctcttttata tctctttgct
     2101 atgctcacta ccaacctata atttgaattt ataaattttt cttttgctac tgaagatgag
     2161 ttgattattt ccttagccag ctctacctct tctcctgctg acaacatttt gtattcctct
     2221 atttccttta aatacatttt catcgcattc gatacatttg atgaacccac acaaatataa
     2281 ttttcataat tattttcttt tttagtatcc ataatcaaac cccttctatt catagaattt
     2341 gctaaaacaa aaataaataa tttaaaccta cataatctac aattacataa tatcccattc
     2401 tttaaatgaa ttgttttgta tattatgtat acaaatttat aaaaactata cctataatat
     2461 tattctactt ctaacagcaa tatcctcctt atatatataa tttattttat tatatgttgt
     2521 tatattctta gttttttgaa tgatatatgt ctaaaaaaag actgtttact aaacagtctt
     2581 tttttatata tatgctcaat atttaatttt catgatgatg attacatcca catccacaat
     2641 tttcatcatg gtgatgatgt tcttctaaat cttttacctg ttgttccatt tcatgaaact
     2701 cttttaccat tatatcataa gttggaagag ctgcctcagt atcgtattct tcccctctcc
     2761 aatcagcgta catcatagca tctccattag ttaaaaccaa tctacctgac atagttatat
     2821 tatccacttc ttctaaaaat tctatttgtt tttgtaaaac ttctccttct aactccacat
     2881 cttcatctaa acattgcact atcattacag gtgcataata atcttcttca tctttaacat
     2941 ttacaacaat atctaaaatt ccttctgcac cttcttcaaa ttctgccaac tcaacatttg
     3001 tatcaatcat atcttgaggt actaataaat cttctattaa atattttatt actctatctt
     3061 ttactaattc ttttgacatt tatttttctc cttccgattc tataacatta tcataatcat
     3121 ttctatatta tagtatacta cattaattgt tttttcaatc tcttacaaaa attatttttt
     3181 attatcttcc ttatattccc ttttttatta caaagttgta atcgcattga tttaaaaaat
     3241 agtttatatt tagtataata gaaatgtagc acatattttt attcaatata ttaataaaaa
     3301 aatatattga aaatgtaatg tatgttaaat ctaggaggaa tattatgaag tttaaaaaac
     3361 aaattagttc tattgttgtg tctactatgc ttgtacttgg gagtatgaac ctttcttatg
     3421 cagacggtac aaatgtagtt acacttggag ctaacttatc agagtctcaa aagcagcaaa
     3481 tgcttaatta ctttggagta aaaaaagacc aagtcttagt tttagatgta acaaatgcag
     3541 aagaaagaca gtatttacaa ggtgttgcaa cagagggtca acttggaaaa gttactatct
     3601 cttgctcata cgttgaacct actaaaaaag gaaatggaat taatgttaaa acagcaaact
     3661 taacttgggt aactagttca atgattgctt caacccttac aacagctggt cttgaagatg
     3721 caaatgttat tgctgctgct ccatatcctg taagtggtac tggagcattg acaggaatca
     3781 tgaaagcatt tgaggatgct tcaggaaaga aacttgatga aactaagaaa gaaattgctt
     3841 ctgaagaatt ggtagttaca ggtgacttag gagatgatat agggcaagaa aaagcaacag
     3901 gtgtaatgaa tgatataaaa acagaaattg taaagaatgg tacaaaagat accaatcaaa
     3961 tagcaaatac aataaataat gttgtaaata attacaatat tactattaca ccagaacaaa
     4021 aagaacaaat taaaggtgta atgaaaaaaa tagctgacca aaattacgat tacaatagca
     4081 tgaaaaatac actagataat gtatcagata atgtaaatga acaattaaag aaattaggtg
     4141 aaagtgttaa gggttctggt atactagata ctattggtgg atggtttagt ggaataggag
     4201 actggttctc tggtttattc tcaggcggag ataaaaaaga tttgggaatc ttaaataata
     4261 ctaatgatga gtctcttggc tctgatgctg taataaattc tacagaaggt gttcaaataa
     4321 atccacaaaa taatactaat tctaacaatg atagtgatac aaatagctca aaaaatgata
     4381 cttcaagtaa taatgaaaat aacgatagtt ctcaaacaaa tgaaaacaac acaacaaata
     4441 attcagaaaa tcaatcaaat aataatgact cagttggttt ctttgaaaaa ataaaaaatt
     4501 ggctttcagg cttattttag aaattaaaca aataaaaaga attgcctaaa atggaaattt
     4561 tctataaaag gcaattctct ttatttacaa taaattttta ttactcactc atttttttag
     4621 atttttctat acatgcacat attgctttta tcacagaagc tctaaaacct tcttcttcaa
     4681 gtactttaac tgcttctata gtagttccac caggtgaaca taccatatct tttaattcac
     4741 caggatgttt tcctgtctca agtaccattt ttgcagaacc cataacagcc tgtgatgcaa
     4801 acttatatgc ttgtggtctt ggcattcctg caattactgc tgcatctgct atagcttcta
     4861 taaataaaaa tacatatgct ggtgcagaac cacttgcacc aattacagct tctataagat
     4921 actctggtac tacctcagta tttccaaaag aattaaatac ttctgtaact gcttctaagt
     4981 cttctttgtt tatattttta tttatcgaaa gagaagacat ggcttcattt acaagagctg
     5041 gagtatttgg catagttcta actatttttt tatcttctcc tataatagac tctatatctt
     5101 ttatagattt tcctgctgct attgtaacta taattttatt gtcatctata aagtctttta
     5161 tctcttccaa aatatcatca tatatatctg gtttaacagc tacaattact atatcagagg
     5221 ttctagttac ttccttcgag tcttttgtag tatttattcc aaaatttgca tctattctat
     5281 cgagagttcc ttgactatat gctgatgcta taaccatatt aggttcaact aattttgaat
     5341 ttactattcc ccctatcata gcgcttgcca tatttcctgc acctatgaat ccaatttttt
     5401 tcactttagt acctccttat tgactaatag taatcgttaa aatttaagct tctttaactg
     5461 catcagattt ttcaaatgga tttggtatac ttactaccaa aattttcact accttattag
     5521 tatagttgac ccaattatga ttgttctttt cactattata gtgtattgtg tctcctggat
     5581 acatttctat ttgttcatct cccaaaaaca ctgtaagagt accctctaat acatacacaa
     5641 attcctcacc ttcatgtacg taacaacaaa tttcctcact actcttactt ggaagaattt
     5701 caattaatct tggtagcatt gttttttcct ttaaactaga tgataaatga taatgtataa
     5761 atgttgagtt ttctacatca aatacttctt tttcataact tcttaataca gctcttttat
     5821 gctcttttgg cttcataaaa aagtaagtta attctacatc tagtacactt gctatttttc
     5881 ctaaggaatc tgttgccaca cttgttaatc ctctttcaag ttgtgataaa aaaccaattg
     5941 acaagtttgt tttttcactc atatccttca gcgtcatttg cttttgagtt ctgagttgct
     6001 ttatttttgc tcctatgtcc ttattcaaaa ccatcacccc taaataattt taattttttc
     6061 ataatataaa tacaaaaata taaatatagg tactgaaccc atctttattt catttaaaaa
     6121 aatttttata agtattacta taatttatca atcttttttt gtctacattt ttacgattat
     6181 ttacgattta tttttcacat atgtatattt aatcttgcca actaaagata cattttttat
     6241 aagcatttac tctttatata ataaaactta agttgttcat gtagtcttct tctggtttaa
     6301 ttattaaaat ttcaaattat attattattt ctacacatta ttacatcctt tagtacaatt
     6361 ataacataat tttacaattt aatgatatct attcaatgtc tttttttact ctacaacata
     6421 ttttatttta gagtttaata ctatatatat tttttatata cagccttgtc attttttaga
     6481 ttgtcaagag ttattgaagt attttatgta gatttagatt ttatttccta atttctcatt
     6541 taacaagaat tgcttatgat aaaaaataat atcatctcaa taagattctt gtaaatatat
     6601 ctatattcta aatataattt ctttttttgt caaaatttct tcattcttta ataattaatt
//
LOCUS       DAELTA010000187         6584 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.188, whole genome shotgun sequence.
ACCESSION   DAELTA010000187 DAELTA010000000
VERSION     DAELTA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.188"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(619..1179)
                     /locus_tag="KPG13_003229"
     CDS             complement(619..1179)
                     /locus_tag="KPG13_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG0986920.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="KPG13_003230"
     CDS             complement(1225..2208)
                     /locus_tag="KPG13_003230"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG0986921.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="KPG13_003231"
     CDS             complement(2221..2583)
                     /locus_tag="KPG13_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0986922.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="KPG13_003232"
     CDS             3826..4473
                     /locus_tag="KPG13_003232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986923.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="KPG13_003233"
     CDS             4748..6385
                     /locus_tag="KPG13_003233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986924.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          691 c          918 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taataagttt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAELTA010000188         6567 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.189, whole genome shotgun sequence.
ACCESSION   DAELTA010000188 DAELTA010000000
VERSION     DAELTA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.189"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1846
                     /locus_tag="KPG13_003234"
     CDS             <1..1846
                     /locus_tag="KPG13_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG0986925.1"
                     /translation="DKNIPIVIVHRTINIENINQIISIENDSDVMVIDAYKESADETA
                     KIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGDKVIDINTVI
                     EIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGIASVDKLGKF
                     IYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNLNNKKLIINK
                     VNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVGESKIIKEKI
                     NIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSLSDTLIESEL
                     FGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQEKEVMRMGG
                     SKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERKEDIPLISKY
                     FLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDYEHLPEQFRF
                     EKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKLQEILKEKDI
                     VLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2107..3306
                     /locus_tag="KPG13_003235"
     CDS             2107..3306
                     /locus_tag="KPG13_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG0986926.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3358..4053
                     /locus_tag="KPG13_003236"
     CDS             3358..4053
                     /locus_tag="KPG13_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG0986927.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4066..4743
                     /locus_tag="KPG13_003237"
     CDS             4066..4743
                     /locus_tag="KPG13_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986928.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            4854..6455
                     /locus_tag="KPG13_003238"
     CDS             4854..6455
                     /locus_tag="KPG13_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0986929.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2619 a          659 c         1255 g         2034 t
ORIGIN      
        1 tgataaaaat atacctattg taatagtaca tcgtactata aatattgaaa atataaatca
       61 gataataagt atagaaaatg attctgatgt tatggttata gatgcttata aagagtcagc
      121 agatgaaact gccaagataa ttagaaaact aggattaatt catattaatc taataccata
      181 ttatccaggt tgtgataagt ctaaatgtga gattggaatt attacgggaa gcagaaacag
      241 cataccacaa aacataaagc aaataattga cataggtgat aaggtaattg atataaatac
      301 agttatagaa atctttacaa aactaaatat atctatagat aaactacata ttattaagga
      361 aaaatacaat gaagatacag taagtggata taggtattat acaactatga ataaaaccat
      421 gaagagtttt ttggagatta tagatgaagg tattgcttct gttgataaat taggaaaatt
      481 tatatattgc aacaaagtat tttctaatct agttggaata gaccaaaatg aaattatatc
      541 taataacttt atggacttat ttagtgataa ggttgtcaaa aaaatatttt ttcaagaaga
      601 tgaagttaat gatgaagtag taaatttaaa taacaaaaaa ctgataatta ataaagtaaa
      661 tgtttatgaa aataatgaac gaataaagag tattataagc attaaaaata taagtgcaat
      721 tcaagtgctt gaggacaaga ttcaaaataa acttcagtca aaaggttttg tatcaaagta
      781 tacatttgag agtgttgtag gtgagagtaa aataataaaa gaaaaaatta atattgcaag
      841 aaaaatagca actacagact tttctgtgtt gatacttgga gaaaatggaa caggaaaaga
      901 aatatttgcc caggcaatac ataatgaatc tctaagaaaa aacaagccat ttgttgcagt
      961 taatctatct tctttgtcag atacacttat tgaaagtgaa ctttttggct atgatgaagg
     1021 aagttttaca ggagcaataa aaggtggaaa aatgggtata tttgaaagag cacatacagg
     1081 aacaattttt ttagatgaga taggtgatat atctttagat gtacagcaga gattgctaag
     1141 agtattacag gaaaaagaag ttatgagaat gggcggaagt aaaattatac caatagatgt
     1201 tagaattata gctgcaacaa ataaagattt aaagaaaaaa attaaagaag gttcttttag
     1261 agaagatttg tattatagga ttaatgtatt acatatagag atacctagac ttagagaacg
     1321 taaggaagat atacctctta taagtaagta ttttttagat gaaataaata gcaacaaatg
     1381 ttttacagag gaatcaatgc aagctttaac attgtatgaa tggccaggaa atgtaagaga
     1441 gcttaaaaat ttaatttatt atattgatac tatagttgag gaagatagag tagattacga
     1501 gcatttgcca gaacaattta gatttgaaaa aaacaatact ttagttaatg aaaatttcga
     1561 ttcaattatc ttagatttca aacaatctaa tttctttgag gaaagtatat gtatattgac
     1621 cagtatagaa acttggaata ataaaaatat attattagga agaaataaac tgcaagagat
     1681 tttaaaagaa aaagatatag ttttaagtgt agaccaaatt agaaaaagaa tagataagtt
     1741 aaagagccat ggtttacttt tatctggagt aaaaaaacaa ggtagtttta taactgatga
     1801 aggaaagaac tttatatcat atgtaaaaat taagggagtg atatagggga ctaaaaaggc
     1861 aaaacaccct aaaaaaatcc ctataatgga tagaaataaa taaaattatc tttaagatta
     1921 agataattca aaaatattga ataaataatc taagaagatt atttttaata aaacagtagt
     1981 aaatatcaac cttactactg tttttatttt ttgcaagatt tattatacaa ttaaaattta
     2041 tattttggca catcaattgc tacataggaa aaatataaaa aattgtagaa cttggaggat
     2101 gttgttatga agtcagctta caaaggatat gaagcctata gttatttaga aaaaggaaaa
     2161 gatatttcat ctattgagat gtgtaaagga gataaaagag ttaaagaata ccttattgaa
     2221 ttagatgata ttcaagaagc taaggttaaa agtatagtgg atgaaaaaat atttatatca
     2281 ttacatgaac atccagtatt atttccagaa cacctagaaa gagatatatt tgaatataat
     2341 agggaaggta aacaaagatg tgcttatgaa gcattatcaa gaggatatta tgatgctata
     2401 tttgataatt taatggatgg tgtatgtacg ataacatcaa attcagggtg gaaatgggat
     2461 gatatattaa tagatttagg catgagacta tgtgatttag ctcatcaaga ttttgttata
     2521 aagtgtgaaa aagtagaaga tatatacaga gctaaaagtg aaggtaaagt agcattgata
     2581 ccaacattag aaggtgcagc accaatagaa aatgaacttg atagaataga tatactgtat
     2641 ggatttggta taaggttaat gggtataaca tacagcgaat ccaatgcatt aggttcagga
     2701 ctaaaagaag agaaagatgg aggtcttaca aatttcggaa aaaaagctgt tgaaagaatg
     2761 aataaaattg gaatggcaat agattgctct cacgttggag ttcaaactac acttgatgta
     2821 atagaatata gcaaggaccc aataatatta agccatgttg gggcaagaag tttatggaac
     2881 acaaagagac taactccaga tgaagtatta atagcatgtg ctaaaaaagg aggggttata
     2941 ggtgtagaag cagcacctca tactacaata acagagaaga ataaagaaca ttcaatagag
     3001 tcatttatgg aacattttga gtatattaaa aatttagtag gaatagacca tgtagctttt
     3061 ggaccagata cagtatatgg cgaccatgta aaattacact cagtactagc tgctaaattt
     3121 gatataaaaa aaataacagg aacaaaagaa catcccaaag tagaatatgt aaaaggtctt
     3181 gagaacccaa cagaaacttc aaaaaacatt gttagatatt tagtaaagca tggatatagt
     3241 gatgaggaaa tagagaaggt actaggtgga aatatactta gagtactaaa acaggtttgg
     3301 aaatagatat attagaaata ttaataaaat ttgaggattt atagggaggt tttataaatg
     3361 gattatttaa aattagctaa tcatccactt ttgtggatag cgagtacagt agcagtatca
     3421 atagtagtgg tacaatccct tatatttgct ataaaatctt gcaaagttgg taaaacaatg
     3481 ggaataacag ataaacaaat aaagtcagct gttaagagta gtgctatttc agctattggg
     3541 ccttcaatga ctatatttgc tggaatggta tcgcttatag taactatggg tgggcctata
     3601 gcatggatga gattatcatt tataggttct gtaatttttg aatcaatgtc agccggattt
     3661 ggaacagatg cattaggtat aactttaggt tctcctgaaa tgacaaaact tgcatttaca
     3721 aatgcagttt ggactatgat attaggttct ttaggatgga taatattcac attactattt
     3781 ggacataaac tagataagat aaccaatgtt atatctagtg gcaagaaatc atttatacca
     3841 ataatatctc taggagcaat gttgggttca tttgcatatc taaatgctga tagggtgttg
     3901 cgttttgata atggaacaat tgcatgtata agtggaatgg ctataatggt aatattgtgt
     3961 atgcttgaaa agaagaaaaa tataaaatgg ttaagagagt gggggcttac aatatcaatg
     4021 tttagcggaa tgataatagc ttcagtaata taagggggga gttttatgag taaagtaaaa
     4081 tctatataca atgaagaata tttacctttt atgataaggt atggaagact aacactatct
     4141 ttgggtatta tagcagcatt agttcctgga ataattttga gctttggatt tggtattatg
     4201 cctccaatat cagcattatt agcaagtaca atggcaattg tcagtatgag tgctccaaat
     4261 tacataatag aaccagtttc ttattcacca atcttaggta tcccaggaac ttatatgagt
     4321 tttctttctg gtaatatatc taatatgagg ctaccatgtt caatagcagc tcaaaaggca
     4381 gcagaagttg aatcaggtac agaagaaggc tcgataatat ctacaatagg aatagctgta
     4441 tctattttag tgaatataag tatactaact ataggggtaa ttttaggagg aagcgtacta
     4501 tctaaaattc cagctgaagt agtagaaaaa ttaaacttaa ttttaccagc tctatttggg
     4561 tcagtttttg gacaagtatt tttacaagat aaaaaattag gtctagtagc aattgtaata
     4621 tctgtattga ctattatatt atcaaagcaa ggtataatac cacagtctct agtagttctt
     4681 atatgtgtat ttggtactat attaattgca agagctatgt ataaagataa gcttagtgac
     4741 taattacaat gaaataatag tgaattttca aatatgaaat ttaataattt gattttcaaa
     4801 tataaaaatt acattaagat tatttaaaaa ttggttttta aggagggaat attttgatat
     4861 atgatttaat cttaaaaaat ggatttataa tagatggtac aggtaatcct ggtttttatg
     4921 gagatatagc aattaaagac aacttaatag caaaaataga ttctaagata aatagcaata
     4981 caaataaaga gattgattgt tatggaaaaa ttataacacc tggatttatt gaccctcatg
     5041 ttcatgaaga aatagtagca atactagatg gtaaatttga aaagtttcta aaacaaggag
     5101 ttacaacaac tataaatggt aattgtggac attctataac accatattct tctgaaaatg
     5161 tatatgaata tatgtataaa aatggtctat tacttgaaga agaaaaaaag tatcttatag
     5221 ataaaaataa atgttggaat aattttacag aatattgtga ccttatatct aaaagtggta
     5281 tttctgtcaa tatgggattc ttgttggggc atgggactat aagatggagt gttatgggag
     5341 gttctaagga tagacctcct acagaaaagg aaaaaaatga aattacagat ataataaatg
     5401 atggaatgaa aagtggtgct tttggaatat ctactggatt ggcatatata ccaagtaagt
     5461 atgctgatat tgatgaactt gttgatattg caaggcagat taaagagcat gatggtatat
     5521 atacatctca tattagagat tacataggaa gatataatgc agtaaaagaa gccatagagg
     5581 ttgggcagaa aagtggtgct agggttcaag tatctcactt aagtccagtt gaaatagaag
     5641 catttgatga gatattaaag gctaggtata atggtgttga aataatggtt gacacagtac
     5701 caagaagttc tgggcattgt atgaagaaaa aaagagtaat tcaatttatt atggcaatat
     5761 catctagtct atttgaatta ggtatagatg gggtaatgga tgctcttaga aatgaagagg
     5821 gaagaacact tatattaaaa gaagcattta tacttggaga tagggggagt ataatacttc
     5881 taaacactaa agatattaat atggaaaaga aatcaatacg tgaaatagct acacaaaaag
     5941 gaatagatga ggacaagtta ttactcgatt tacttttaga tggagacgaa gaacttatct
     6001 tttgtttagg aggaatgtat agagctgatt tcccagataa actacatgat gataaaataa
     6061 ttgataatcc atttgttatg gttggttcag attgtttgtt tagtgtgggt ggggatatgt
     6121 catggtttga actacaaaga agaggagctt ttccaatttt cttcaatatg tacagaaagt
     6181 ctggtgttag attggaagag attgtaagaa gagttacttc attaccagct aggcaattta
     6241 aaataaaaaa tagaggtatt cttaaagaag ggttgatagc tgatatagca gttattgaca
     6301 tggataacta tagttatcct aggtcagaag atattgattt ttcaaagcca caattactag
     6361 ccaatggtgt tgaatatgta attgtaaatg gaaaaattgc gctagaaaat ggagagatta
     6421 ctgagaataa atgtggtgaa gtattaaaaa gataaggttt taaaaataat aggatttaga
     6481 aagtaaataa atatctaagt aagtaaagac gaagtttgag atttatttaa ataaaaatca
     6541 tgcttcgttt ttatttatag tattaat
//
LOCUS       DAELTA010000189         6544 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.190, whole genome shotgun sequence.
ACCESSION   DAELTA010000189 DAELTA010000000
VERSION     DAELTA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.190"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            84..2777
                     /locus_tag="KPG13_003239"
     CDS             84..2777
                     /locus_tag="KPG13_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0986930.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            complement(3051..3986)
                     /locus_tag="KPG13_003240"
     CDS             complement(3051..3986)
                     /locus_tag="KPG13_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG0986931.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4030..5202)
                     /locus_tag="KPG13_003241"
     CDS             complement(4030..5202)
                     /locus_tag="KPG13_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0986932.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(5189..5653)
                     /locus_tag="KPG13_003242"
     CDS             complement(5189..5653)
                     /locus_tag="KPG13_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986933.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(5668..6510)
                     /locus_tag="KPG13_003243"
     CDS             complement(5668..6510)
                     /locus_tag="KPG13_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG0986934.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         2241 a         1167 c          956 g         2180 t
ORIGIN      
        1 tagcaaattc attttttcta ttaattacaa aactactcct atttgtgtta taatattgca
       61 ttaagtaagg aggttaaaat cttatgaata acgataatga taaaaatgga aataaaatca
      121 ggagaaaaaa agttagctca tctggtaata caaataaacc aattaataga acttcagcta
      181 acaagactcg ttcaacaaaa aacaagaaaa aatctaaaaa aagcgataaa tttaaaaaac
      241 taagagtatt tgggatagta tttttagttt tactagttgt tggtacagca ggtactgctg
      301 gtctagtatt tgcgtcacta agagatgtaa ctccagttac agaagctgtt ttagataaac
      361 agacgaacca aacaactact attaaatacg ctaatggaaa aacattatct acagctccaa
      421 gtgtaaataa aaagacaccc gttccattag ataagatttc cccttatctt caacatgctg
      481 taattgcaat agaagatgag cgtttttatg agcataaggg tgtcgatatt aaaggtttat
      541 ttaggtcagt attaaagacc cttacaggta ctaagcaagg tggtagtact attcctatgc
      601 aggtatccaa aatgctgtta acaactgaac agcaaactat acctcgtaaa ataaaggata
      661 tttactatgc acatgagatg agtaaaacag taagtaaaga taaaatacta gaaacttatt
      721 taaataactt cttcgttggt agaggtctag caggtgctga ggctggtgca cgtggttatt
      781 ttgataagtc tgcagctgac cttactctag cagagtcagc acttcttgct ggttcaacta
      841 aaaatccatc aagattttca gcctataaaa cttctaagtt agagggaaat gaaactaagg
      901 aggatttaga gaataaatta ttattttttg ttaacacaac tgatgatgat ttagatgacc
      961 ctactcaagt ggactttgat atgattgaaa aaataaaatc ttgggagctt atatctaatg
     1021 atacttatag acaattaaaa gcaggtacat tagttgttag aaaggctgta agtaatcctg
     1081 aagctaaaaa aagacaagaa attgttttaa agaaaatgtt agaattgaaa tatataaaac
     1141 aaagtgaata tgatgaagca atcaaagcta aaatagaaat aaaattgcct caatcatcag
     1201 ataaagtttc ttcatctgta gaagacctta tcgaaagtga agttataaat gctttaatgg
     1261 aacaaggaca tacaaatgat gaagctcaaa acttattcta caatggtggt ctaattgtaa
     1321 acacaactat agaccctaaa atgcaagatg ctttagaaga agaatttgat agaaatagca
     1381 atttcccagg acatatggtc ggtcctgatg gagttagcca accgcaagct gcaatggtaa
     1441 tcttagatta taaaaatggt gaaataagag cactagctgg aggaagaaat ataagtggta
     1501 gaaaaacttt aaatcgtgct actaaccctc atcagccagg ttcttctata aaacctttag
     1561 caatatatac gccagctatt gatactctaa aaataacaca agcaactgct ctaagtgatt
     1621 ctcgaggagg atataagttt gaagaaaata ataaatggaa tccaagaact actactgctg
     1681 gtcatggttc tatgagttta cgtaaagcac ttgctaaatc ttcaaacacg atagctgtca
     1741 aaactgctga aatgcttgga gactcttatg atgaatgtgt agatataatg atggattact
     1801 taaagaactt tggtataaca actttaaaga ataatcactc aggttcatct gaagcaagtg
     1861 atagaaaatt cccatcattg acactaggtg gtatggcgaa tggtattact ccacttcaaa
     1921 tggctgctgc ttatggtaca ttggcaaatc acggtattta tgtagaacct tctattttta
     1981 ctactataac tacctttgat ggtcaattat tagttaaaaa tgctcctgaa gaacataaag
     2041 ttgtagaccc tgaagtagct tatgttgtta cagatatgtt agaaagcgta attactgaag
     2101 gtactggtgg tgttgctaca cttccaaagg gaatgcctgt agcaggtaaa acaggtacta
     2161 caaatagtgc atatgatgct tggtttgtcg gttacactcc ttattatgtt ggtgcaactt
     2221 atattggaga tgatgctggt agaaaagatg attctggtaa cactataaaa cgtagagaag
     2281 taccacatgg aagtactagt actgcaaaac tatgggaaaa aattatggaa aaaatacatg
     2341 ctaatttaac ggttactgaa tttgaagttc ctaaaaatgt atattttaca aaaattaatt
     2401 tagaggatgg aggaaagcaa tcttctggct ctaaagcagc atttattgaa ggtactgctc
     2461 ctactaaagt aagttctcaa ccatcatctg aagatacaaa aaaaccagac aataaccaac
     2521 cagaagaaaa taataataac aataataaca caggtgataa tggtggcggt tctacacctc
     2581 ctgataatgg cggaaacaat ggtggtggct ctactactcc tcctgataat ggcggaaata
     2641 atggtggtgg ctctactact cctcctgata atggcggaaa caatggtggt ggctctacta
     2701 ctcctcctga tggtggtgga aataatggtg gtggctctac tactcctcct gatggtggtg
     2761 gaactccagc aacttaataa aaaatagaat ttaaataaaa atataaggag ttgtctcaag
     2821 gtgattaaaa aaatcacttt gaaacgctcc tttttttata attacaatat attatttgta
     2881 ttcataaaaa agagaatccc aataaccaga atcctccttt ttatataaac aaataaatat
     2941 tttattttta aagtttttaa ctaagtgctt tattcttact tttattccta attgtttata
     3001 tattcttaaa tagttatatc acaaattata taaacttaat atttttctaa ttactgtata
     3061 ccaaatgttt gaaatctatt taatttgcca tatctcttaa taagcatttc ccactcttct
     3121 ttatcataga aatcacactt atttctacca aatagcttgg caacttcaat tgcaaatctc
     3181 ccagctgaat ctaaatcact aaaattagtt gcacctgtag cacatccagc aacaggcatc
     3241 tctgttgtaa ttgctactcc tactattggt gcatctgtag ctactgctgg ttgtaaaata
     3301 ctatttaagt gatacagatt atttccatat ggtgttatat cttgaataga caaagggaat
     3361 acaaatggag atttccctgt tacagtctgc ataatatcta aaaggttatc actagttttt
     3421 aatatgtagc cttctttaac agttggcgat attgcaaatc ctctagtatt gattactcta
     3481 tttcctttag tagtatctat agaaagtatt gcatcacaac tactatcaac ttcattttca
     3541 ttcatagtca tcatatcaat tggagaatcc ataaaaggag taggatagtg ttctttagtt
     3601 ggagcatctg ggcaaatatg agttgaaatt acaacatcac catctaatat atcccctttt
     3661 cgttgcatat ccaaaagttt agcagccact gcaattgcta ccaatgctcc atcaccatct
     3721 gagacaaaac ctataacttc tggtcttgct ccaatccctc ctagtctacc taaaatacca
     3781 agagtaggag catctccacc ttttaatttt ccattttttc caggaattgt aagctttact
     3841 aaatctgttg aacctttact tgaagacaat tcttttacag ttacatctac ttcaccataa
     3901 ccttgtaagt aagtttttac ttcttcccca ttagcatttg ctttatctaa aatgtcataa
     3961 acctctatta cttgttttaa tatcatgtct aatccgattt cctttcttaa tatattttaa
     4021 aattatatat tatttactat acaaaatatc tactccacac attgctaaca ccttaattgc
     4081 ttttataagt gctactctgc cttcttcacc accagctgca acagcaaatt catctgtatg
     4141 agttgcagta ttttctttta attttatata tgcttgtatt gcaggtatct catgagttaa
     4201 atttcctgta tcagttgaac ctataccttg acttaaatct ctttcaacat agtcttcacc
     4261 caaaacttca aaattttgtc taactatatt cactaattca gaatcattct taatctcttt
     4321 gtatatttca tctaattgaa ctatttctac attagttcca gtagaaattg cagctccttt
     4381 agcacaattt ttaagcattt cacaaatctc atttaaatat tctatactta aggctcttac
     4441 attaaatata gctgtagatt tttcaggaat tatattatgt atgctacctc catcagttat
     4501 aattccatgt actcttgcat aatctttaag atgtaatctc atgctattta cactgttaaa
     4561 tagtgctatt actccactta aggcactttt cccttcccaa ggaaaagcag cagcatgtga
     4621 tgccttacca gtaaaatcaa attttaaatt aacttgagca aatgatatat catcaggcat
     4681 tgaagcatca cttggatgta aaattaatgc agcatctaca ttattaaata caccttcttt
     4741 aatcatagtt atttttccac ctactctttc ttctgcaggt gttccaaaaa cctttacagt
     4801 cccttctata ttatgttttt tcatgatttc ttttaatatg atacctgcac caacagaact
     4861 tgtccctatg atattgtgcc cacatgcatg tcccattcct ggaagtgcat catattcagc
     4921 taaaaaagca actgttttcc cattagttcc atttgaatac acagcttcaa aagatgtttc
     4981 taatcctcct acacctttag ttacattaaa ttcattttct tctaacaaat cacataatgc
     5041 tttttgtgac ttatactcct caaatgctaa ttctggattt gaaaataaat agtcagaaac
     5101 tttttcaaat gatgtctttt tctcttcaga taataacttt atttcctctt taatttttgc
     5161 ttttaattca ttcatattta ctacctcctt aaagcattcc atttaatctt aaaactgttt
     5221 gagatataaa agcagaacca aagtaagtag atgtcataac tataaatgat ataactacta
     5281 acttccaact caaagtcttt aatatatcta attgattacc tacagagatt cctgcaaaag
     5341 ctaataatgg tgttactgta gacataaaat tgattttccc tacatttgtt gctatgatat
     5401 cccccactgg agatattgga agcgataaaa caaatgcaac taatgtagcc catgcaaaac
     5461 ctggtaaatt aggtttttta acaaaattct ttattataat agcaattatc accaacaaaa
     5521 atccagatac acttccaatc aacaaatctt gagcctgagt cttaagtgct atcatttgtc
     5581 caaagcaaat aataatatat ataaacaata ctatttttat ctgctccata atagtcttaa
     5641 aagttttttc catatatata tcctccttta caatttatta cttgttcttc ttgtttttct
     5701 tcctctaaat ctttcaataa ctggagctac tttgctataa tagtagttgg ctactggtaa
     5761 agataagaat aattctatat atattccagt aacagctgta atcaatccac tagtagctga
     5821 atatgctaaa atctgctctg acatttgtgg aactgtattc attaatgatg ctgttgcagc
     5881 tcccatcatt gaaccactac ccattcctga tgccattgct aaagcatatg gatgaaatcc
     5941 taaaagtaca cccactggtg ctaaaaatga aaataataat gttccaaata tactaccact
     6001 tatgtaagtt cctagtgttc ctataccttc tggtgagttt aatccatact tatctccaat
     6061 aattcctagt gctgtgtcac gacataatga aaaagtacca cctaatgcac ttcttccaag
     6121 acctaatgct actccaacag gtaatgctat caacattgta cccatatgac caaattcttg
     6181 taataaaagt gctggtcctg cttgaagtaa tataccaact ttaggccctg cattaattcc
     6241 aaacattatc cctaatggat atagtgtcaa agataataaa tatggagata catttatttc
     6301 attatcccct ataaactttt ttaaggctgg tataactctc cctaataaat gaggagaaac
     6361 agccattcct atgaatatag cccatagtat actaagtaaa gttacattaa ttcccataac
     6421 tttaaatgaa ataggtccaa ttaattctgc caatattgtg attataaagg ctgctattat
     6481 ggcatttcta atccctgctt tttctttcat ttctatacct ccttgtaatt tttataattt
     6541 tctt
//
LOCUS       DAELTA010000190         6540 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.191, whole genome shotgun sequence.
ACCESSION   DAELTA010000190 DAELTA010000000
VERSION     DAELTA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.191"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            468..1412
                     /locus_tag="KPG13_003244"
     CDS             468..1412
                     /locus_tag="KPG13_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG0986935.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1434..2060
                     /locus_tag="KPG13_003245"
     CDS             1434..2060
                     /locus_tag="KPG13_003245"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG0986936.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            2094..3143
                     /gene="hisC"
                     /locus_tag="KPG13_003246"
     CDS             2094..3143
                     /gene="hisC"
                     /locus_tag="KPG13_003246"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG0986937.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3146..3724
                     /gene="hisB"
                     /locus_tag="KPG13_003247"
     CDS             3146..3724
                     /gene="hisB"
                     /locus_tag="KPG13_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG0986938.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3726..4331
                     /gene="hisH"
                     /locus_tag="KPG13_003248"
     CDS             3726..4331
                     /gene="hisH"
                     /locus_tag="KPG13_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG0986939.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4328..5050
                     /gene="hisA"
                     /locus_tag="KPG13_003249"
     CDS             4328..5050
                     /gene="hisA"
                     /locus_tag="KPG13_003249"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG0986940.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            5032..5790
                     /gene="hisF"
                     /locus_tag="KPG13_003250"
     CDS             5032..5790
                     /gene="hisF"
                     /locus_tag="KPG13_003250"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG0986941.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5818..6486
                     /locus_tag="KPG13_003251"
     CDS             5818..6486
                     /locus_tag="KPG13_003251"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG0986942.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2678 a          553 c         1269 g         2040 t
ORIGIN      
        1 attattacaa tttttcccga aatttcattt tttatacata aaatttccaa attaggtaaa
       61 gattaaattt tttattgtat aaacaaaagt tacatataaa atattttatt caatttttta
      121 aataactatt gccaattttt gaaaaatata ttattattaa tagtaacaaa aaactgtaaa
      181 tttagaatat taataaaaat tatttgggag gaaattatca tggtgagatt aaatatagta
      241 aaattaaata caaaattaaa tcatcaccat catcatagat taaagggatt gtaatatgaa
      301 attaattcat agacacaagc cctgttttta gtgtaattgg tcttgtatct atgatgggaa
      361 gtaattttaa gagaaaatta tagccatata ggtataaagc ctatgtggct tttttattgt
      421 tttaaatttg caaatataag agtaaattta atgaagaggg tgagaatgtg aaatttttga
      481 ggattgaaga tgaggttatt tattctaaaa aaagatataa attaaaaagg gaaatagata
      541 aacttttttt ggatgaagaa tattttcaaa tagaaccaca attatttgaa gaatatgatg
      601 aatttacaac tataaataat aaaattccag aagaatctat ggtaaaggtt gtaaatggaa
      661 aagtaatggt acttagagct gatataacta caagtataat aaaaagcctt gtaccaagat
      721 gggaagatgg tttaaagctt aagctttttt ataattcatc aatatataaa aataaaaata
      781 cagttggaat aaaagagata agacaaatag gttgtgagta tctaggagaa gcctctgtag
      841 aagcagatag agaagtagtg aaattagctc ttaagatact tgaaaaatac aataataatt
      901 ttattctaga ggttgggagt agcaagtata ttcatggttt gttagaagaa ttaaatttaa
      961 ataagaattg tgaaaatcaa attaaaaatt tactatatac gaagaacact catgaactaa
     1021 aagtatatat tgaagaatta aaaattaagc gtgaagtaaa agagttatta tcaaatattt
     1081 taagtttaca agggaattta tataatgtta ttgaaaaatc aaacaagttc tattgtaata
     1141 ataagatgaa gcaagctctt gaagaattaa agcaagtaaa taatttaatt gaggaatgta
     1201 attttttaga taaggctaga tttgatttat caatgattac tatgcttgac tattatgaag
     1261 gaattatgtt tagaggatac tacccaaact catataaaga aattcttagt ggcggaagat
     1321 atgattcttt aactaaagag tatggaaaag aaattccagc cattggattt acactaagtg
     1381 ttgatgagtt aatgaagtat gtatataagt aaagtttaga aggagggatt gctttggatt
     1441 atttgactat agcactagca aaaggacgaa ttgaagggga atcatttaaa aaatttaaaa
     1501 aaatgggtct aggagacagt atagacacag atacgagaaa actaattttt aaagatgaag
     1561 aaaataaaat tatatatata catgtaaagc catcagatgt agttacatat gttgaaaaag
     1621 gagtcgctga cctcggtata gcaggtaaag atactatttt agaaaatgag acagatgtat
     1681 atgaaatata tgatttaggt tttggaaaat gtaaatttgc tgtagcagga ttaaagggag
     1741 atagcatata tagagaagat gaatatttaa aagtggcaac aaagtatccc aatatagcaa
     1801 aaaagtattt taaagagaaa ggtcaaaaaa ttgaaataat aaaattaaat ggttctgtag
     1861 aactagctcc aatagtaggg ttgtcagatg taattgtaga tatagttgag actggcaata
     1921 cacttaaagc aaatggtctt gaaatattag aagacatatg taatataagt gctagaatta
     1981 tatcaaatag agctagctat agatttaaat atgaacaaat tcagaatata attcgtttat
     2041 ttgaagaact tgataattaa aatataattg agctttataa ggagggattt aagttgagag
     2101 aaaaagaaag cataagagag ttaaggggat atgaaccaaa tcacgtaaac tgtaaagtaa
     2161 aacttgatgc aaatgaagga agtaagaggt tgtttaaata ccttattaaa gaaatttcag
     2221 atagtgatat agatttaaat ttatatcctg aagattctta tagtgatttg aaagaatcta
     2281 taatagatta cataaatatt tctggagtaa ataagaaaaa tcttctagta ggaaacggtt
     2341 caagtgaaat aattgattta ataatacata cttttgtaga taaagatgaa gtaatattga
     2401 gtttctctcc aagttttagt atgtattcta tatatagtca gataaatggg tctaaattta
     2461 taggtgttga aagtgatgaa aatttagtta taaatataga taatgtgatt gaaaaagtaa
     2521 aggagaataa tcctaaaata gtaattgtat gtaatcctaa taatccaact ggaacaatat
     2581 taaagaggga agagataata aagcttttag attcaactaa tagtctagtg gttttagatg
     2641 aagcatatat ggattttgga gaagaaagca tgttaagtga tgtttttaag tatgataatc
     2701 taatagttct tagaacttta tcaaaggcat ttggattggc tggaataaga acaggatata
     2761 tgttatctaa tagtagttta ataaattctg ttgagaaagt aagacctcca tataatttaa
     2821 attcattatc agactttatt gctacaagag cacttagaaa taaagatgtg gtaaaggctt
     2881 atataaagga agtaaaagag gaaagagaag ttttatatga agaaatgatt ggtatgggaa
     2941 ttaaagcata caaatcacaa gcaaatttta tattatttta ttctgaaatc gaaaatttat
     3001 cacaaaaact tattgataga ggtgttttaa taagaaaatt tggtggaaag ttagaaaatt
     3061 attacagagt tacaattggt gataaagaag aaaattctat gtttgttgga gctataagag
     3121 atattttgaa aaaggagaaa taggtatgag aatttggaag gtagaaagaa atactcttga
     3181 aacacagatt ttagtagaat taaatattga tggttctggt aaagctgaaa tagatactgg
     3241 aataggattt ttagaccata tgcttacctt aatgtcattt catggcaagt tcgatttaaa
     3301 agtgatttgt aagggtgata catatgtaga tgaccaccat agtgtagaag atataggtat
     3361 agctataggt gaagcattta aaaatgcact aggtgacaaa aaaggaatac gaagatattc
     3421 taatatctat atacctatgg atgaatctct atcaatggta gcaatagata taagcaatag
     3481 accttatctt gtatttaatg ctaaatttga tacccaaatg attggtagta tgagtactca
     3541 atgctttaaa gaatttttta gagcttttgt aaatgaatcg agagtaacat tacatatcaa
     3601 tctcttatat ggggaaaatg accatcataa gatagaaagt atttttaaag catttgcaag
     3661 agctttaaaa gagggtagtg aaatagtttc taatgagata gcatcttcaa agggggtttt
     3721 gtagaatgaa tataatagta gactatggtc ttggaaatat agattctgtg tctaggggtt
     3781 ttagaaaagc tggaatagaa actaaaattt ctagtgatat agatgaaata aaacaggcag
     3841 attcacttat acttccaggt gttggagctt ttagagattc tataagtgcc ttagataaac
     3901 taggattgat accaataata aaggagcatg tttctaaggg taaatttatg ataggtatat
     3961 gtctaggaat gcaattactt tatgaaaaaa gttatgagta tggagaatat gaaggattag
     4021 gacttataaa aggaagtatt gataaactgg atataagttt aaaagtgcca catatgggat
     4081 ggaataatct aaaatttaat aaggcaaatg atgatatact taaatatata aatgaagatg
     4141 attatgtata cttcgttcac tcatattatg caaattcatc aaatgaagaa ctaattgcat
     4201 tttcagaata tgaaaagaaa atccctgcta ttgttagaaa aggcaatgta tatggtatac
     4261 aatttcatcc tgaaaaaagt ggagaagtag ggttaaatat attaagagca tatggggaga
     4321 tgataaaatg ataatatttc cagcaataga tataaaagat aataagtgtg taagactcac
     4381 acaaggagaa tttgacaaag taaatgtata ttatgataat ccattagaag tagcatataa
     4441 atggaaaaat gaaggagcag aatatataca tatagtagat ttaaatggtg ctagaagtga
     4501 atttggtgta aatacaaaaa taattgagga catagcaaac aatatagata tacctattca
     4561 agttggcggt ggagttaggg ataaggaaaa agtcaaaagt ttaataaatg ctggtgttac
     4621 tagagtaata ttaggtagta tagcgattga aaatttgaat ttagttgaag aattggtcaa
     4681 tgaatataaa gagaaaattg tagtttctat agatgctaaa gatggaaaag tagcagttag
     4741 gggatgggaa gttgtaagca atgtagactc attaacatta tgtaaacaac ttgaaaagat
     4801 aggtgtacaa actattgttt atacagatat atcaaaagat gggatgttgc aaggtccaaa
     4861 ttttgacata tatgaaagaa tagcaaagga aacatcacta aatgttattg cttctggagg
     4921 cgtaacttct atagaagacg taaaaagact aaaagctatg aacttatatg gtgcaataat
     4981 aggtaaagcg ctttatgata aaaagataga ctttaaggag gcacaacaat tatgcttact
     5041 aggagaataa taccttgtct agatgtgaga aatggaagag ttgtaaaggg gaaaaaattt
     5101 aaagatatag tagatgttga tagtccagaa gtacttggaa aattttatag tgattgtggt
     5161 gcagatgaac ttgtattcta tgatataact gcatcaaatg aagagagaaa gacatcttta
     5221 gaatttgtga caaaagttgc agaaaatata aatataccat tttgtgttgg tggaggagta
     5281 aataaattag aagatttcac tgatatttta agaaaaggtg ctgataaagt ttctataaat
     5341 tcatcagcag tcaaaaatcc tgaattaata agggaagctt cattaaaatt tggagcacaa
     5401 tgtgttgttt tatcgataga tgcaaagaaa aatgaagagg gttcatggag cgtatacgta
     5461 aaaggtggaa gagaaaaaac taatctggat gcaattgaat gggctgttaa aggtgtagaa
     5521 cttggagcag gagaaatcgt tgtaaatagt atggatgaag atggaatgaa aaatggatat
     5581 gatatagaat tattatcaaa gataacttct ttagtaaatg tacctgttat agcatctgga
     5641 ggcgctggaa aaaaggaaga tttttatgag gcggttaata aatccaatgt agatggaatt
     5701 ttagcagcat ctgtttttca ttttggcgag ataaaaataa acgatttaaa gaaatattta
     5761 aaagacatgg gtgtagaagt aagattataa gttaaatact aaaaggagat tagaaatatg
     5821 gataataagt gtaacaatgt atacagtgat gaagtagaga aatttataag aagtataaag
     5881 tttgatgata aagggttagt ccctgttgtt gttcaagagg tagttagcaa agatgtactg
     5941 atgttagcat atatgaataa agatgtgata aagaaaacct taaaagataa agtagcttgc
     6001 tattttagta gaagtagaca agaactttgg gttaaaggtg agacatctgg taatactcaa
     6061 aaagtagtta agatgtctta tgattgtgat gtagatacta tactactttt tgttgaacaa
     6121 actggtgtag cttgtcatac aggcaattat tcttgttttt acagagactt gtttgatgat
     6181 agttcaaaaa tggagcttga agtacaaaca aatatattga aagaattata tgatttaatc
     6241 aatgaaagaa aaaataatcc agttgaaggt tcttatacta attatctttt tgaaaaagga
     6301 atagataaga ttcttaaaaa agtaggtgaa gagagtagtg aggtaattat agcatcaaaa
     6361 aatactgata aaagtgaact tatttacgaa attagcgatt tagtatatca tacattggtt
     6421 ttaatgatag aaaaaggtgt tgaaattgat gaaataaaaa aagaattact taaaagaaga
     6481 aaatagaata agatagatta atgggtaata agaaaatata ccacttttaa taaaaagtgg
//
LOCUS       DAELTA010000191         6489 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.192, whole genome shotgun sequence.
ACCESSION   DAELTA010000191 DAELTA010000000
VERSION     DAELTA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.192"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(159..1889)
                     /gene="lpdA"
                     /locus_tag="KPG13_003252"
     CDS             complement(159..1889)
                     /gene="lpdA"
                     /locus_tag="KPG13_003252"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG0986943.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2056..3102)
                     /locus_tag="KPG13_003253"
     CDS             complement(2056..3102)
                     /locus_tag="KPG13_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG0986944.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3115..4101)
                     /locus_tag="KPG13_003254"
     CDS             complement(3115..4101)
                     /locus_tag="KPG13_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG0986945.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4126..5088)
                     /locus_tag="KPG13_003255"
     CDS             complement(4126..5088)
                     /locus_tag="KPG13_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG0986946.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5126..6079)
                     /locus_tag="KPG13_003256"
     CDS             complement(5126..6079)
                     /locus_tag="KPG13_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG0986947.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
BASE COUNT         1821 a         1329 c          670 g         2669 t
ORIGIN      
        1 acaaggattc tatattctat atttttccat aaaaaatacc ccttattatg caaataaatt
       61 atttgcataa taaggggtat ggctaagtaa gtgttttaaa attttatgat ataaattaat
      121 attaataata gacttaaatt aataatttat gtttagattt attttttagg taagtgtaaa
      181 cattctccat ttacatctgc caaagcttcc attaaagctt ctgaagtaca tggatgacca
      241 aatatcaact cacttgcttc atcagtaggt atctctaatg ctttaaatga tgctgcatga
      301 tttataagtt ctgctacacc acatccaaac atatgtatac ctaaaatttc tccatattta
      361 gcatctgcta ctaccttcac atatccttgt tcttgaccag aagctaaagc tcttccattt
      421 ccagcaaaat taaacttacc aacttttaca ttatattttt ttctagcatc ctcttctgta
      481 ataccaactg atgctacttc tggaattgta taaacacaac taggtaatgc acctaagtca
      541 acttctttat tcacacctag agcatttgaa gctgctacct ctcccatttt aaaagcagca
      601 tgagctaaca ttacacctcc agtaacatca cctactgcat atatgcttgg tatacttgtt
      661 tccattttac tatttacaac tattgaacct ttatctattt taatatctaa gtcctctatg
      721 ccagaaagat tagcttctct tcctatagca tatagacata aatctgcttt tattggctct
      781 tcaccttcaa tgcacactaa tatgttattt ccttcttctt taaattcaga aactttcttt
      841 ttagttaata catttatacc ttttttacta agagaatatt ttaaagattc gcttaactct
      901 ttatccattc ttgggattac tctatcttcc atttctacta tagttacttt agaacctctt
      961 gaattgaata tctctgcaaa ttcacaacca atcactccac caccaattat tactaattct
     1021 tctggcactg tttccaaatc taatgcttct gtactagtta ttattaaatt tgattctatt
     1081 ccttttatag gtaatatcct tactttggaa cctgtagcaa ttattatatt ttctgtatct
     1141 aaaacttttc catctgataa aattacttta tgctcttctt taacactagc tttaagatta
     1201 aacacatcta catctctact ttttaataat ccgccaacac cagctgtaag ttttttaact
     1261 actctatttt tatattttat ggccttctta atgtcctgtt ctttatctac agttactttt
     1321 acacctctct ttgataattg gtcaatttct tctaatattt ctgctgtttt tatataagtc
     1381 tttgtaggga tacaacctct atttaagcaa gtacctccta gtatattttc ttcaactaaa
     1441 gcaactcttc cacctaaaag agctgctttt aatgctgata aatatccccc aggtcctcct
     1501 cctattacca ctacatcata atcatgatta cactcttttt tgtttatttt ttcttcttta
     1561 aattctatat tttgattctc ttcaactata tcttcttttg aaacatttcc agataaatat
     1621 ttagctttta cttcctcttg attttcccct ttttctgcta ttacacctat tactgtaaat
     1681 actggtaata cttcaccttc ctctttatgt atgattacag caagtgtacc ttctccttct
     1741 gactctatct ccatattaac tttatctgtt gttatttcta gtataggttc tccaatcttt
     1801 acttcttcgc cttcttgctt tagccaagat actatagtac cttcttccat ggctactcct
     1861 gcttttggca ttattacttc tactgacata ggctctcctc ctatttattt tcttatattt
     1921 taattaagca aatcatattc acatatagtt actgttaaaa ataattctta atatataaaa
     1981 gctttaaatt tactgaaaca attataataa tataatctat tcaaactatt tatatcagta
     2041 acaaaatatc aaaacctata caagcattga taaaggattt tctaacaatt cttttaaatc
     2101 ttttaagaat tttgcagcta ctgttccatc tattactcta tggtctgatg ttaatgataa
     2161 gttcattata ggttttattt tagcctctcc atttactgga acaaacttat cttgtgtagc
     2221 acctacccct aagattgcac ttgaaggcat atttattata ggtgtaaatg tagttatacc
     2281 atacattcca acattactta ttgtaaatgt attaccttct tggtctgctg gcataagttt
     2341 ccctgtcttt actttttccg caagttcttt gctttctttt gctatttctt ttaaagactt
     2401 tttgtttgca ttttttacta ccggaacata taagccttca tctaaaccaa ctgcaatagc
     2461 tatatttaca tctttatatc taaatatacc ctcatctgtc catgctgaat ttaaggcttg
     2521 atgatttgga agtattttag atactgccat tactattaaa tctgtcatag ttaattttac
     2581 tccagttgat tcttttactg tgtcgattaa ctttgctcta agtaccttca attctgtagc
     2641 atctacttct atattgaatg taaatacagg agcactaaaa taactttctg acattcgttt
     2701 tgctacagta gctctcattg gatttggctt ttcaaatatt ccttctactg tgttaaacat
     2761 gcttgaattt tcattttcaa ttttcagctc ttttttctca ctagatttag atgtattagt
     2821 cgatacaata gtttcttttg cagttaattt ttccacatca cactttctta tctttccatt
     2881 atatccagta cctacaatag tttctaaatc tatatttaaa tcttctgcta ttcttctagc
     2941 taaaggagta gtatttgctt tattatcttt taaataattt aacacatcta ctaaatgaat
     3001 ccttccattt tctccacttc caatcagcct atctaatttt atattgtctt ttcttgcctg
     3061 acttctagct gctggtgtgg cttttatttt atttgccacc atatctatcc ctccttatct
     3121 aacaattaaa gattttattg cttcttctat ttcatcaact cttgggacaa ctgctttttc
     3181 taactctgga ttatatggta ttggcacatc ttttccacaa attcttttta ctggagcatc
     3241 taaataatca aatgattcac tttctgttat cagtgctgat atttctccac ctagcccacc
     3301 agtcttagct gcttcatggc aaattaaaac tctcccagtt ttacatacac tctttactat
     3361 agtctcttta tctaatggat ataaagttct taaatctata atttcaacat ttatattttc
     3421 ttttgataga gtctctgctg cttcttcaac actttgtaac attcttccat aagtaattac
     3481 tgtaacatca gtaccctctc ttttaatatc tgcctttcca atttcaatta catagtcatc
     3541 ttctggcaca aaaccttttt ttctgtataa caatttattc tcaacaaata tgactggatt
     3601 attatctctt atagctgctt tcaacaatcc cttagcatct gctggtgtag aaggtgcaac
     3661 tacttttact cctggtacat gacaaaacca agcttctaaa ctttgtgaat gttgctctgc
     3721 tgaaccagtt ccagaacctc ctgggcatct aacaaccatt ggaacttgtg cttttcctcc
     3781 aaacatatat ctcatttttg ccgcttgatt tacaattgca tccatagaaa ttgttacaaa
     3841 gtccataaac attacttcca taataggtct taagccagtt gctgctgcac ctgctgccgc
     3901 tcctgctata gctgcttctg atataggagt atcccttact ctttctggtc caaattcatc
     3961 tatcatacca actgatacac caaatgctcc tccatatatt cctatatctt ctcccataaa
     4021 tattacattt tcatctcttc tcatttcttc tgacattgct tcctttattg cttgtgcata
     4081 tgttaattcc cttgtactca tttttatttt cctcctttac cattcttagt ctgcatatac
     4141 atcctctaat aaagactcta tttttggatt tgggctattt tgagcaaact ctactgcatc
     4201 ttctattgat tgctttgcaa attcttgaat cttatctaat tcatcttcat tagatagatt
     4261 attttctata agataatttt ttaaaaattc aatagggtct tttgctttcc atgattctat
     4321 ttcttctttt gttctatata catttgcatc tgattttgaa tgacctaacc atctataagt
     4381 tctactttca attaatacag gtccttctcc tcttctacac ttctcagcag ctttttgtac
     4441 tgtttcgtat acttctattg ggttatatcc atctattgat attccttcta ttccataaga
     4501 agctgctcta gtggcaatac tttctatatt catatgacgt tttatgcttg ttgacatacc
     4561 gtataaatta ttctcacagt aaaatattat tggcaatttc catattgaag ataaatttat
     4621 tccttcatga aaagtacctt catttgatgc tccatcacca aaactacata aaactatttt
     4681 gcctgttttt ttatactgtt gtgtaagtgc agctcctgga gctattgtaa gacctccacc
     4741 tacaactcca tttgctccta agttacctga ctcaatatct gctatatgca ttgagcctcc
     4801 ttttccctta caaaatccag tttctttacc cataagttca gccatcattt tatttaggtc
     4861 tattcccatt cctataaatt gactatgtcc tctatgagtt aatgatacta aatctccttt
     4921 ttctaaagcc attacagccg ccacacttga cgcttcttga cctacagata agtgagttgt
     4981 tccatgaacc atacctcttg caaaaaacca actaactttc tcttcaaatt ttctagcctg
     5041 attcattctc ttatacattt caagcaatgt ttcttttgat attgacatat acattccctc
     5101 ctgttaactt ttatctatct ttctattagt cacaatcttc taataattta agtgctaaac
     5161 tgtgattaat tgctaaacta tttataaatt tcgcttttaa tgatgccttt attgcctcta
     5221 ttttttcatc tcctgaagcg acacctattg ttctttttat cttttttaaa tcttccagtt
     5281 tcactcctaa tactctttga ttaaattcaa gtatagaagt ttttccttct ttatcaaagg
     5341 cttgtaaaca aattgcacct attgagttgt atttttttaa atcttctata tctgcttcat
     5401 tcatgtatcc agaagccatt attgttgagt ttaaactcat aggatttcct atacccacaa
     5461 ttgctatagt tgttttatct attaaatcta gtacttcttt aattttttcg tctttgcaaa
     5521 gtttctcttt tgtagacaag tcagacataa cagctggagc atgtaataat ttaaaatcac
     5581 ctccaaaagc cctcgctaaa cttacggcta tttgatttgc atgaatatct atttcattgt
     5641 ctccaatccc tcctaataga ggtataaatg taacattttt acagttactt ttttccacat
     5701 atcttggtat ttcttttagt gttgttccta ttgaaacacc tattatatca ttttccttta
     5761 ctactctagt taaatattca gatacagctc ttgctagttc ttgtttttgt gttagtgcat
     5821 cctgtttatc atctactact attacttctt ttaatttata ttttctctcc aaacttctct
     5881 ctattttttg gtaatcattt ttcaaaacat caactatctg tatttttact attccttgtt
     5941 caaaagcttc ttttaatatt cttgatattg ttggtcttga tattcccaat tgatttgcta
     6001 tttcctgttg attcaaatta tcttcgtaat aaagtgaaca acacttcatc attaatctta
     6061 agtctccaat ttttttcatt tgctttcctc caaaatcaag cttttcaatt atctttacat
     6121 ttgttcaatc attttactta atttcagtaa cattatagaa ggggttcata taaaaagtca
     6181 actgtttttt tgtataacaa aaaaaaggtg atacttttct tgtaccacca ccttcataaa
     6241 tagttgaaaa attgcttctt agtgcatatt atatattact tctaattgtt aaatatcatc
     6301 tccataaatt aaaaattaat tgtccactta taatgcttta aataacaata acttattaaa
     6361 tcacttctaa aaaaccaacc atttcatcat ttaaagtctt cttatatttt tattattcgc
     6421 ttctctaaaa attcttcaat aatcataaat atataatagt ttttataggg aacatttaca
     6481 atagacctt
//
LOCUS       DAELTA010000192         6478 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.193, whole genome shotgun sequence.
ACCESSION   DAELTA010000192 DAELTA010000000
VERSION     DAELTA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.193"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    150..361
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            524..1813
                     /locus_tag="KPG13_003257"
     CDS             524..1813
                     /locus_tag="KPG13_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG0986948.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1867..2946
                     /locus_tag="KPG13_003258"
     CDS             1867..2946
                     /locus_tag="KPG13_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG0986949.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2967..4511
                     /locus_tag="KPG13_003259"
     CDS             2967..4511
                     /locus_tag="KPG13_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG0986950.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4674..5216)
                     /locus_tag="KPG13_003260"
     CDS             complement(4674..5216)
                     /locus_tag="KPG13_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG0986951.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5364..5648)
                     /locus_tag="KPG13_003261"
     CDS             complement(5364..5648)
                     /locus_tag="KPG13_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG0986952.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5711..6475)
                     /locus_tag="KPG13_003262"
     CDS             complement(5711..6475)
                     /locus_tag="KPG13_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG0986953.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2258 a          802 c         1127 g         2291 t
ORIGIN      
        1 gaaatttaaa aaaatacatt atataaatat agtaatagta tgaaaattta catataaaac
       61 taaaaaatgc aagtaattga ctttttaagt tgacaaaata ttgcatatat attatgatat
      121 aaatcataaa ttaactacaa atcaatatta tatattcgta gaagaagaaa gtagcataaa
      181 ggaattttac agagagttag atttggctga gaactaatat tggaatttat gtgaaaagga
      241 ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct taagaaaatg
      301 agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat tcgtctcttg
      361 gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa aactatatat
      421 taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa attgtaaaat
      481 atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag aagaaaaaat
      541 taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag ctatgttttc
      601 agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg agttggcaca
      661 gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt tttctgcaat
      721 tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat ctatgataac
      781 aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag gggtttctct
      841 attaggttgg tttggagttc aagtaggttt ctttgctcaa aatgcacaga taataataaa
      901 agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg gtctcttgat
      961 gatgagtact gcaatttatg gatataaggc aatggagaag ttgagtgtgt attcagtacc
     1021 atttttatta gttcttatga tgctaactat attcttagct tttagagcaa atggaatttc
     1081 agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc tgtcaatgag
     1141 tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta aaagtagaag
     1201 agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta tgataatagt
     1261 ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt ttattacatt
     1321 aggaattgct ataccaggta taatagtact tacattagca caatggacta caaatacaag
     1381 taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc ctgagaaagt
     1441 attaactata gtccttggta ttatagcaac attattggct gtatttggaa tatatgaagg
     1501 ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg gtgtatatac
     1561 agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag tattatataa
     1621 accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa cttacctttc
     1681 aacatatgga tttataactc ttactactat agcaccttta gatggtttta tagcagggtt
     1741 tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc aagaaataga
     1801 caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat atgaggagaa
     1861 agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg tgcagcagtg
     1921 cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa acaagccatt
     1981 aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga tgctttggta
     2041 gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact ttcagaagta
     2101 gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt ggatgttata
     2161 attcctatag aaataggagg gataaactca cttatgccta tagcagttgc tgctaaaaaa
     2221 ggacttccta tcttagatgc agattctatg ggtagagctt tccctgaagc tcaaatggta
     2281 actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga aaaaggtaat
     2341 tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag aactttaact
     2401 gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc tcaagttaaa
     2461 aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt tttacttgaa
     2521 aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgtg tggatacaag
     2581 ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt tactagagga
     2641 catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt attccaaaat
     2701 gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga tttaatagca
     2761 gtattagatt tagaaactgg atttccaata actacagaga gaataaaata tggctctcgt
     2821 gttatggtag tagcttttcc ttgtaatgaa aaatggagaa cagaaaaagg aattgagaca
     2881 gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga attacaagga
     2941 aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt ggaggaacaa
     3001 atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt aaggtggcaa
     3061 ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata gatgaaagta
     3121 agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt acaaatgcta
     3181 ttgtagaaag aaaaagactt aataatgtgg ctatgataag aataggaaag ccggccacta
     3241 ctgccattct tccatttata gattggccag aagacttaag agaaaaaata gaattagatt
     3301 caatattggt atctggtgga tatgagtttg atggcagaaa aataaatgaa ctttgtaaag
     3361 atgaagttgt agagttttgc aatagaataa aaggcaaagt ggaaagtgtt gcaataagtg
     3421 gagtatttgc tccagtaaat aaacaacaag aagaagaagt tgcaaaatgg gttagagaaa
     3481 ttttagggga tattcctata tctttatcaa atgaaatagg taatataggt atattagaaa
     3541 gagaaaatgg agctatttta aattctgcat tgtctcaagt aggtaaagct gtatctttag
     3601 gatttgaaaa agcacttaaa gatttaggta tagatgctca aatatatttc agtcaaaatg
     3661 atggaacatt aatgaattta gagtatacaa tgaaataccc aatttttaca attggatgtg
     3721 gaataacaaa ttctataaga ggagcttcct tcttatcaaa agtaaaaaat gcaatagtat
     3781 tagatgttgg tggaactaca agcgacttgg gtgtactata taatggattc cctagagaat
     3841 catctattcc agttacaatt ggaggagtac atacaaactt tagaatgcca gatgttttaa
     3901 ctattggtct tgctggtggt acatgtgtaa aaggcgaaaa agataatata aagataggtc
     3961 ctgaaagtgt gggatataga ataactgaag atgcaataat atttggtgga gacactctaa
     4021 ctttatcaga tgttgttaca aaattagata tggcgtttga agatagaaaa agcaatgttg
     4081 aaaacttaga taaagagtat tgtaatgagg tatataagaa ggttatttta aaattagaag
     4141 atgctgtaga ccaaatgaag actagtcaag atgatgttga attggtttta actggtggag
     4201 gaagtataat tgttcctgaa aaactaaaag gcataagcaa tgttataaga cctcctcatt
     4261 atactgctgc aaatgctatt ggtgcagctt taggacaaat aagtggagat gtagaaaaag
     4321 tatacagttt agataaaatg tcgagagaag atgccataga agatgcaaaa aaagaggctg
     4381 taaataaagc tctaaaagct ggtgctaaag aagatactat agaagtatta aatatagaag
     4441 atgttccact tgcatacctt cctggtaatg cacttcttat aaaagtaaaa gttgttggtg
     4501 atttaattta aagaataaat ataagtattt ccagaattat ataaatattt ctggactcat
     4561 ttttaaactt attttatcaa taaaaacagc ataacttttg actatattct tataagtttc
     4621 cgaacagccg atatatacct attttttgag gtttatcggc tgtttgttat cttttatcgc
     4681 attccaacta tttttcttac cgtatttgct gtacgaattg taatcttatc acttacattt
     4741 gaactggcag tctttgacca ccaatttgtc tttttataat ctttacgact aaaagaccaa
     4801 aagatagcat ttttataatg atatactttt tcatactctt ctttaggatt tccaacctca
     4861 tcataaaact cgtcataaga taagtcagga aacataaaaa taagattatc atatatttct
     4921 ttgttgttat ctccccacca attaggagca ttatttagta tatcttttaa ctcttcttgt
     4981 gaaataataa aaactggtat ttccaattca aactgttttt ttatcattaa tcttattgta
     5041 tttgagataa ccttgctgtc atctacatca cttaaaaaga caacattacc actattaagg
     5101 tatgtagaaa cttctgaata accaagttct acaaagcttg cttttaattc agacataatt
     5161 atcttgttct taccacttat attgatacct cttaataatg cgatataact tttcatataa
     5221 taatttcacc tctaaacatt cacatttttc tatgtagtac aaacttgaat ttgcatcttt
     5281 tataattctt tcaaaacttc ttcatatgta atttttttaa tataaaaaaa tcggtgctta
     5341 atttgtaccg atttttgttt ttcctagtaa agaactcgtt tttttgcttc tgctatagct
     5401 ctagcagcac tttttgcttg cattcttttt tcttcttctt ttttaaattc atttattgtt
     5461 tcatttagat tcttatcatg ttcatttttt tctttatctt cgttttctct gtatactaat
     5521 atataatcta ttattgattc gtttattatc tgagatatac ttttattttc attaattcct
     5581 attctcttaa cttccttaag tagagtctca tcaatatgaa ccgttgtttg ctttgtattt
     5641 tttgccacct taatcacctc ttaaaaaaaa ttctaacatt aaaatttttt ttagtcaata
     5701 aattttaata ttaaaattct ttaaatgttt tatttgattt tctaaaattt tctattgtat
     5761 ttatcatctt attttttata ttttcaccta gtacagaaga ctttagtcta ggattttcga
     5821 tatggtcata aattggttct aaaaattcaa cttcgacagg aacagagtta attttaccaa
     5881 taggttggta tccttcataa gtatcttttg aattttttat tacgagtgga acaatagggc
     5941 attttgcttt atatgctatc tttaaagctc cacttctaaa ctccgaaacc aatgaatttg
     6001 gttctttaat ccatgttaaa tctccttctg gaaacactgc catactttga cctgtaagta
     6061 tattttgaga ggcttgggct atactcttta taccctcacg attgtttttt ctgtttacat
     6121 atacacatct tagtaatttt gcccattctt taaatatagg tatatttctc caaactggtt
     6181 catctgcaat tacacatcca attggtcttt ctacacttgc tgttagtatg aagctgtcaa
     6241 gcatactaga atgatttaca acaaatagaa gtggttcttt tggcagtgtt tccttaccaa
     6301 ttatattaag ctctatattc actatatcta aagatttttt agcttgtttt tgcataaatt
     6361 caaatttttc ctttaagcta aatttatcag gattcttttt aattttaata agcctaggta
     6421 tagaagatgt aaaacctata agttgatatg tagcaaattt taagtaatta ttcataaa
//
LOCUS       DAELTA010000193         6376 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.194, whole genome shotgun sequence.
ACCESSION   DAELTA010000193 DAELTA010000000
VERSION     DAELTA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.194"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..3347
                     /locus_tag="KPG13_003263"
     CDS             168..3347
                     /locus_tag="KPG13_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0986954.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3632..4396)
                     /locus_tag="KPG13_003264"
     CDS             complement(3632..4396)
                     /locus_tag="KPG13_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0986955.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4552..4785)
                     /locus_tag="KPG13_003265"
     CDS             complement(4552..4785)
                     /locus_tag="KPG13_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986956.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4859..6352)
                     /locus_tag="KPG13_003266"
     CDS             complement(4859..6352)
                     /locus_tag="KPG13_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG0986957.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2370 a          907 c          742 g         2357 t
ORIGIN      
        1 catagtttat aagataattc aataaaaatc taatagtaaa ttgttattta cacatatatc
       61 gtgatagata aaagctattc atatctgcct taattttagt cttaaattta gagtgtaaat
      121 tgaatgcatt tagattataa tatatacagt gaggtaaggt gaataatatg aaaatagata
      181 tgatatataa actaataaga agaaatacag aaccatcaag atggtcaaga ggtgtaaaat
      241 attatactaa tcaattagtt gattatatag acatagatat tgatgaccta caaaataatc
      301 tgttgacatt tcgtggtgga gtcaattctg aagataatta tagcttttat tttaattcaa
      361 taactataga tataaagcac tttaagataa ttgaaaatga ttgtagttgc gctgactgtc
      421 aatcaaaggg caaaagtatt tttatatgta agcatatagt tgctattgtg ataaaaagct
      481 taaaacaaat aaaaaataaa aaagatgttc ctcctattct aatagatata ttaggtgcag
      541 ataaggataa gctttctaat acaaattcta aaaccagctt agatttaagc aagcaattac
      601 tttctttgtt agacaatact aaaagagaaa gggttaattt agatgtattg ataaatgtgg
      661 attcaaataa agcaattgct gaatttaaaa taggaattaa taaaatgtac attttaaaaa
      721 acattactga atttgcatat tcaaaactta atggatatga attagatttt gggaagaaat
      781 ttacttacaa ccccaacact cattattttg ctgatgaaga tgagaagtta gtagatatta
      841 tagaagaata tgggacaaat ctttcataca acttttcttc atcacattca aaaaaattta
      901 tggagataaa tgcaagctct ttaaaacaat ttttaaatgc tattaagtac aaggatttta
      961 cccttatttt ttctaaagag gaatatcatc caaagatatt aaatgatact ctacctgtaa
     1021 ggtttgattt taaaaatata gaagataaaa tagtcttata ttctaaagat tcactacctg
     1081 ttccacttac taaaaaaaat gatgtacttt tatatgatgg caatatctat ttattatcac
     1141 ataaaaaagc tcatgattat tcaaaaatat atcaaatatt atcaaagact aaagagttaa
     1201 aatttgataa agaggattct aaggatgttt taggattgct agttcctaga ttaaaatcta
     1261 tatctcaaga agttcattta gatgataaca taaaaaataa tataacaaaa gattttaaat
     1321 ctgagttcta ctttgatatg attgatggca acatatgttg tgatgtaaag tatatatatg
     1381 atgatgaaga taaaaaattt gtgctcccca acatacaaaa ggaagctact attgaaaata
     1441 aacttacttc ttcacaattt acaaaggaaa ataatcatta tgtgtttaaa ggaactgacc
     1501 atgacctatt tacattttta gatttacaat tagaatctct aaaggaattt ggagatatat
     1561 attattctga gaagtttaaa ttgaaaaaaa tatataacgc ttcatcaatt caagcttcaa
     1621 tcaatcagac taaccagaat tacctagaat ttaattttaa tatatctgat ataaatccaa
     1681 aagagtacaa ggatattttg aaagcttttc aagaaaaacg tactttttat aaacttaaag
     1741 atggaaactt tatagattta tcagaaagag aaacaaaaga tttttttgaa ttagttgaaa
     1801 atctagatat aatggataaa tcaaaaaata ataaaattca taaaaataaa gctctatata
     1861 taaatgatgt aatacgtagt aaaaatttaa agtttataga tggtaaaaaa gaactagaca
     1921 gtatatgtga aaaatttaga aattttgata gcataaattt agaaattccc gcaaatttaa
     1981 atgcaaacct tcgtgactat caaataaatg gcctcaattg gtttaaagtt ttagattatt
     2041 ataaatttgg tggtatatta gctgatgaaa tgggtcttgg taaaacaata cagactattg
     2101 catttttatt atcattatct aataaaaaaa gtcttattgt tacaccgaca tcactcattt
     2161 ataattggaa aaatgaattt gaaaagtttg ctccagatat aaaagtttta ttgatacatg
     2221 gaaataaaag agatagagaa aaatgtttta tggaattaga aaattttgat gtaatactta
     2281 caacatatgg tactcttaga aatgatttag acaaatatag tgaaataaag tttgattatt
     2341 gtatattaga cgaagctcaa aatataaaaa atccagtggc tttagtaact gagtctgtaa
     2401 aaagtataaa tgcagaaaat aaatttgcac tcactggtac acctatggaa aataatctac
     2461 tagaattatg gtctatattt gattttataa tgcctggcta tctatatagt aaagcaaagt
     2521 ttcaggaatt gtttataaat aaagaagata atgttaaaaa cctgaaaaaa ttgataaaac
     2581 cttttattct tagaagaagt aaaaaacaag ttatgaaaga acttccagat aagattgaaa
     2641 aaaacttttt tgttgaattg aataaagagc agaaaaagat atatagtgta tattcaaaag
     2701 atatacaaga taaaatgaaa gataaaaatc taaagaaaga taaaatagtt atattctctt
     2761 atttaactaa actaagacaa ctttgtttag acccaagtat agtagtaaaa gattacaata
     2821 aaaaaagttc aaaaattgaa acatgtttag aaatacttag ggatagtata aacgaaaacc
     2881 acaaaatatt attgttttca caatttactt ctgtgcttaa gaacataagt aaggaacttg
     2941 ataagtataa aattaaatac cattatatag atggtaaaac taatgccaaa gaaagacttg
     3001 aacttgtaga tgagttcaat aatagtatgg ataaaaaagt ttttttaata tctttaaaag
     3061 caggcggaac tggtttaaat cttacatctg ctgacatggt aattcacttt gacccatggt
     3121 ggaatccatc agtagaaaat caagctagtg atagagctca tagatttggt caaaaaaatt
     3181 cagtacaagt tataaaattg attgctaaag ggactataga agaaaaaata ataaaattac
     3241 aagaaagtaa aaaagagctt ataaatcaat ttataaatgg agaactttct aatgaaggtg
     3301 tattaaaaag tctatctgat gaggagataa taaacttatt taactaaatt atttaatcct
     3361 acagcttaac tatatgaatt tgttctaaca tttatatagt taagctaaaa tatctgaata
     3421 aataaaattt aatattgtta tttaatccca actttacaat ctaatgatat ctctttctat
     3481 acatattgca tatacaaatg taataacttg actattagaa tattttgttt taacaatcct
     3541 atttcatcat aataatgaag tatatgtaca ctcacttttg taagttttgc aatccatttt
     3601 acagttttca ttattgtaaa ttttggttaa attattcctc taaaaatttc tttgctacat
     3661 ttgaaaaatc taatccattc gataaaattg caggatgacc acctttatca aacaaataca
     3721 ttttaccatt tttaatcttt tcaagaagta atgaaaaagt attttcaaaa aaatcactag
     3781 atattagaga tacaaactca tcttctctac ttcctgttaa aagcacctct ggttgcatag
     3841 tttctagagg cttatgaaaa aactttccta tagttttata atgttcaaaa atagcttctg
     3901 tatcattatc tacaactttt tcccagtttt ctccttgatt ataaatataa aaagcttttg
     3961 caccatcatc ttgcttagat gcttcacgct ctattttcac attttgtaca aatgactcta
     4021 gaggaacttc tccttcaaaa ctatctgcaa ttaccttatt cactaaatca ggtctctcaa
     4081 gtgcaacatt taatgcaaca agagctcctc cactactacc aattatgttt acttttttat
     4141 agttcattgc ttctaaaaaa catatcactt gcatagcctc atcaaaccat aagtcagcag
     4201 aaaatttatc gaccatttga gattttccat gacctacaaa atcaattaat attactttat
     4261 attcatcttt ataaaaattg attatatcat taaacatctt ggaagaggct gtatttccat
     4321 gtaaaaaaag taatggtgta ccatccccaa tttcattata gtagcactgt ttgtcgttat
     4381 agttaaaata tgccatatat ttcccccctt taatatccct ataggtatat atttattact
     4441 tatatttaaa atgactattc ccttagccac ttaaaaatag tataacttag aatgtagata
     4501 aaatcaacat tccttgctaa tttatctatt gtttgtctaa tttattataa tttaacttct
     4561 gtaaaatttc ttttttgtac gaaaatattc cttccattta caacaatcat tcaaatgaag
     4621 tataacatga cgagtaggag gcataagcaa aattccagca atatctttat tcccccaaaa
     4681 gtccaacaac caaatagaat cttcatgctt tattactcct ccagtcacca aaatcctttc
     4741 taagtcaatt ggagagacct ttcgtttcat atcttcagct ttcaaatcct taacaacttc
     4801 tattgtgttt tttgcaactt catttcgata acaaagcaat tcatcaatat tgactaaatt
     4861 atcgtatatc tcttttcact ttaaccattt ttttctttat aggtatctcc ttatattttt
     4921 ttaatatcaa atctccctta taatttaata tatctacata tgaacataaa tcttgaactt
     4981 caattacacc tatatcatca ttagttatac cttttatatt actaaatgca ccaactatat
     5041 ctaaaactct tattttcttc ttcttaccag catttaaata taatttaaca acttcacttt
     5101 ctatatttag ttttatatct ttacttttgt aagaattttt atcaactttt tttctctttg
     5161 atactttttt taggattcct tttgaatatt cttcaaacaa aactttgcca tgtgtaattc
     5221 tacttttttc tatatctttt aattcattta ttttatatcc tatatacgtt tctatattct
     5281 ctatatattt ttcatccttt tcagttacta tagtaattgc ctttccacta tttccttttc
     5341 tcccagttct acctattcta tgtatatagt tttctttatc ttgtggcaca tcataattta
     5401 ttactaaaga aatatcatca atatgtattc ctctggacgc tacatcacta ctaactaata
     5461 tattaaattt ttggttttta aaatccttaa ttacaaaaat tcttctttct tgtgataaat
     5521 ctgcatgtaa ctctcttatt aaaaaaccct ctttactcat cttgttatat aatttagata
     5581 ctttctcttt ggtattacaa aatattatca cactctttgg attttcgctg tatattaatt
     5641 cttttaaaag gatgtacttc tctcttccat ttgccttgat tattttgtca tctatttgtc
     5701 ttgttttttg attagtatcg ctctcatttt cttctatatt tataacactg taatctagca
     5761 tatatttttc acatatatat tttatctctt catctattgt agctgaaaaa agacttacta
     5821 ttttctcctt aggtaaattt aataatattt tttccatttg ttcaacaaaa cctttattaa
     5881 acattttatc tgcctcatct attactaaat actttacatt ttccaactta atagaacctc
     5941 tatttatatg gtcaagtatt ctgcctggag tagctacaac aatatgaacc ctttgtttca
     6001 attcagctat ttggtctttt atagattgtt tgccaaatat agcactacat ctcaccttct
     6061 ttagtctccc tatatctgat atctcatctt ttacttgcaa ggctaattct cttgttggaa
     6121 ccactataag agcttgaata ttattgtaat ctacatttat attctcgcat aaaggtattg
     6181 caaaacttgc tgtctttcca cttcctgttt ttgacctaac tactaagttt tgacctttta
     6241 ataattttgg aataacttct ctttgtactc tagaaggtat attatatcct aaactcttca
     6301 atgacttaag tatcttttca tttaacttaa atttttcaaa tgtatatttc atatattacc
     6361 accttattat atttat
//
LOCUS       DAELTA010000194         6325 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.195, whole genome shotgun sequence.
ACCESSION   DAELTA010000194 DAELTA010000000
VERSION     DAELTA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.195"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            201..1526
                     /gene="cdeA"
                     /locus_tag="KPG13_003267"
     CDS             201..1526
                     /gene="cdeA"
                     /locus_tag="KPG13_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG0986958.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1621..2415)
                     /locus_tag="KPG13_003268"
     CDS             complement(1621..2415)
                     /locus_tag="KPG13_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0986959.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2416..3291)
                     /locus_tag="KPG13_003269"
     CDS             complement(2416..3291)
                     /locus_tag="KPG13_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986960.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3295..3663)
                     /locus_tag="KPG13_003270"
     CDS             complement(3295..3663)
                     /locus_tag="KPG13_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0986961.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3846..4505)
                     /gene="deoC"
                     /locus_tag="KPG13_003271"
     CDS             complement(3846..4505)
                     /gene="deoC"
                     /locus_tag="KPG13_003271"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG0986962.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4541..5728)
                     /gene="murJ"
                     /locus_tag="KPG13_003272"
     CDS             complement(4541..5728)
                     /gene="murJ"
                     /locus_tag="KPG13_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG0986963.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5805..>6325)
                     /locus_tag="KPG13_003273"
     CDS             complement(5805..>6325)
                     /locus_tag="KPG13_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG0986964.1"
                     /translation="ENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHI
                     ANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKA
                     NWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINIL
                     KEGLRLITIQNK"
BASE COUNT         2084 a          989 c          699 g         2553 t
ORIGIN      
        1 atacatatat ttctaaagat tttcttaaga aacagttaga ttttactaat ataagtttat
       61 taattagcat tatactaatt tatcttaata tgattttttg ttgattttaa gctgtttatc
      121 gtataaataa aatttttctt gactatatag ttactatata gcttattata tatagagagt
      181 aataaaagga ggtaaaattt atggaaaatt tatttacaag aaaattcact acttttgaat
      241 ttctaaaatt tgtttctcct gcaattatat ccatgatatt tatatctttg tacacaataa
      301 tagatggcat ctttgtatcg acattagttg gttctgatgc tcttgctagt ataaatattg
      361 tactacctat aattaacctt gtttgtggat ttggaataat gatggcaact ggtggaggtg
      421 ctatcgtttc tatacgtatg ggtgaaaata gacaggatga agccaactct acattttctt
      481 ttatagtttt gttttcattg attgttggga ttttattcac agtaatctca tatttcttta
      541 tcaaagaaat atctattttg cttggtgcaa cagataagtt attaccatat tgtataactt
      601 atggtaaagt tatgatttta tgtacaccat tttatatttt aaaatttata tttgagtact
      661 ttgcaagaac tgatggaaat tctaaattta gtttatttct atcagtcatt ggtggtgtaa
      721 caaatataat attggattat gtatttatta aatattttgg aatgggtctt ttaggagctg
      781 cagttgcaac tgctataggt attattttaa cttgtgtttt aggtattatt tacttcttat
      841 ctaataaatc tacactaaaa ttaagaaaac caaaaaccga ttttagactt ataagagata
      901 ctatgataaa tggttcttct gaaatggtta cagaattatc tacaggaatt acaacattct
      961 tatttaatgt agtagcttta aaattagcag gagaaaatgg acttgctgct cttactatag
     1021 tattgtatgc tcatttttta atgacatcag tctatctagg attcgctgct ggagtgtctc
     1081 cattaataag ctataatttt ggtgctgaaa acagtgataa attaaaagaa acatttaaac
     1141 attctctaaa atttatattt gtttcttctc ttttagtgtt tatttttgct ttagtatttg
     1201 caccatttat tgttagggtc tttgtaagtc cagataacac agtatttaaa ctagccttac
     1261 aaggattaaa aatatttgca tttgcttttt tgtttgttgg tataaatata tttgcatcag
     1321 gattttttac agcatttcac aatggaaaaa tttcagctat tatatctttt agtcgtgcct
     1381 ttgtttttat aatcatagga atcataattc ttcctcctat gttaaacatg actggattat
     1441 ggcttacagt tccatttgct gaggttataa ccatatttat atctattcta tttataaaaa
     1501 aatataaatg tagatataag tattaaaaat taatacttaa aatcaacatt tattaaaact
     1561 aataaacctg ctttaaaata caaatttaca aatttgattt ttaagcaggt tttattttag
     1621 ttataataag tcatttgcat actctttaat tggtgctttt ctaagtaata aaattataaa
     1681 tattatgcaa ataactataa ttcctacttt tgttccaaat aaaataaaag gattctgaaa
     1741 tgaataaata attttttcta aatacaatag cattttatgc atatttttag ttgcaaaatt
     1801 aactatataa attatcatta atatcggtaa tcctatcaat atagtaatac tagtaacttt
     1861 tctaactcta tatgtaaaag ctccaagtaa cactcctata aaacctatac atatttcatt
     1921 tatacatatt tgtacaaaat aatttaaata agttagtggc atacttgatg ttgaaggatc
     1981 tatcagtgct gatatagatg tccaaatcat atctctcttt agtaaaactg atagttctat
     2041 actgtaacct gttataattt ctataaataa cttcgataaa aaaatcaaaa ctatacctaa
     2101 aatagaaatc acaaagctta aaataattaa atctatgaca atagctttta tacaactttt
     2161 tctatctgct cttatactta aagctcctga aaagtcttta tttgctatat ttactccata
     2221 cacaaatagc agtatagtta tatatgttaa aataggattc acaatcgtgg ataaattagt
     2281 tttagatgct tctccaaaaa tcttgagaat aaacactggc aaaatattca taacaattcc
     2341 cacaatcata aatataatta tatacaattt agtattttta ttaaaaaatt ttatatatgg
     2401 ctttaattca ttcaattaca attcctcctt cttagtcatg ttaacaaaca tatcttgtag
     2461 ccctatgtat cctacatcaa tatttgaatt tttaagtatt ttttcatcac tttcactaaa
     2521 atcaccatag taaaaataag caactgcatt tccaaatgcc tttttaggtt ttatattttt
     2581 tatacattct aatttttcta actcttcttt atttccagta atataatgag ctttttgttt
     2641 tatgacatca atttcttcat ctataataat ttttccctca tttaatatta ttacatgctc
     2701 cagtaaatca tctatttcat caatcaaatg agttgatatt attattgtcc ttggattttt
     2761 tatataacta tctaaaatta tgttataaaa ctcttgtcta tttactgcat ccagaccaat
     2821 tgttggctca tcaaatatgg tgatagctga attagaacaa atacctatta tatttgaaag
     2881 tatagttttc atacctcttg acagttgttt atactttttt ttagtattta aatcaaaata
     2941 tttgcataat tcttcttcta agcctttatc ataactttta taaaaactag aagaaaattc
     3001 aaatatctga cctactttaa aatctggact ataaaattct ttttccctaa cgatacaaac
     3061 ttcttccaaa acttttatat cttcttttgg gttctttcct aatatttcta tctcaccttc
     3121 attttgaatt aactgattta ccattatatt taataatgtt gtttttccag ctccattctt
     3181 acctaataaa ccatatatct tatttttttc aaagcttaga gacatattat taaaaatatt
     3241 ttgcttctta aaactgtgag ataaattttc tatcttaata gaactactca tatattaatc
     3301 ctccttgaaa ttaattataa tatcaaccaa ttcttctcta tttatttcta atttgttagc
     3361 ttcttgaagt aggtttttta caaaattatg tttaaaattt tcttttctag cttcttttat
     3421 aatagttttt gcaccatctg atacaaacat ccctatcccc ctcttcttat ataaaatatt
     3481 tttatcaacc aatatattta taccttttaa tactgtagct ggattaattt tataaagctt
     3541 tgaaagctct gttgttgatg gaacctgttc ttcttctttt attccatttg atagtatctc
     3601 atcttcaatt gctcgtgcta tctgaataaa tataggttct tcattattta atactaattt
     3661 cataggttcc tcctttctcc atcagttagt tactcaacta attaactata taacttataa
     3721 tatcttgaaa aatattttct gtcaatacta aataaaaaac agagggaaaa ttccctctgt
     3781 tttttgttct ttatatttat aattaaattt tgatttttgt aatcctatcc cataagttaa
     3841 aaactttaat aatctgttgt atcttcattt tttaatattt tacatattga gctagttcct
     3901 aacctttctg ctcctgcctc tataaatttt tctgcatcag aaattgattt aactccccct
     3961 gcagctttta tttttacatt ttttccaaca tgctctttca taagttttat atcttctaaa
     4021 gtagctccac ctgttcccat tccagtagat gtttttataa aatctgaacc tgacattgtt
     4081 actatttcac acatttttat tttttcatct tcatctaaat aacatgtctc tataatgact
     4141 tttaatactt tatctcctat agcttttttt atttctttta tttcttttcc tatattatca
     4201 taatctttat tttttatatc tgaaatgttt acaaccatat caacttcatc tgctccattt
     4261 tcaatagcat cttttgcttc aaatactttt gtagcagtag tttgatatcc caaagggaac
     4321 cctatcactg tacatatctt tattttacct tttagatatt ctgatgctct tttcacatat
     4381 gatggtggta tgcaaactga tgctacactc atatctacag cttcatcaca taaaatttta
     4441 atatcttccc atgtagttgt agcttttaaa atagtatggt ctactgtttt taatatatgt
     4501 ttcataaaat cctcctaaat ttataattaa tataattaga ttactttttt aaatgcaatt
     4561 ttctctttgc aatgtttagt atcatatcaa cctcttcaac tttaaaaata atcattagta
     4621 gtgtataaat tccacctcca actataactg aaatagctag tgatacaaac tcattaaatg
     4681 ttcctactcc caaaattcca aatataaatt tatacgtaaa gtatgataaa acacccataa
     4741 ttaaagatgc aactaccgat ttcaaagttg cttttattat tttatcttgt ccaaaatatc
     4801 caacttttct ctttaggttc aagaagagta acaatataca tgcaattgat gatgaactag
     4861 ttgcaagagc aagaccacca tgagccattg gttttattaa aactaagtcc aatactatat
     4921 ttacccctac agatataatc ccatttacca taggtgtttt tgtgtcttgt aatgaataaa
     4981 aaacctttcc caaaatatct cttaagccaa atgcagtcat accaactgca taaaaaatta
     5041 aggcagttgc tgtcatttgg gttgccctcg catcaaaagc acctctctca aaaatgattc
     5101 taacaacagg agttgcaaaa aaaatagaag caactgaaat aggaatcata gaaattatta
     5161 tcatatttat actggattta actgaactag taaacttctt ttgattattt tctgcactta
     5221 acttagataa cataggatac ataacagata ctattgaagc tgtaaatgtc cctgtaacaa
     5281 aaccatttaa tctatctgca taagtaagtg ctggaatgct tcctttaact aatgttgagg
     5341 ctaaagttgt atctaccaat gagttcactt gattaacagc aacacctata aatactggtg
     5401 aaagtaatgc tagtaattta ataaggctat catctttaaa gtttaagtag tataagtatt
     5461 tgtagttcgt ttttttaaca aaaaacatgt aaaagagcag ttgaactacc atagcaacca
     5521 cagcaccaat tggaagtatg tatggtccaa atatggtact taacattatt gaaataatta
     5581 ttactatatt atagggtata cttccaaatc caactacaat aaagttctct ttaatttgta
     5641 aaaatgctga cataacactc gtaataccta tgaatattat tcctgttctg aacctcccat
     5701 gactaaagtc acagggttcc tgcttcatag aattttgcat actaaaatat gtcttactaa
     5761 ttctccacag gctatccccg tagttcctac ggttcttaca tacgttattt attttgaatt
     5821 gttattagtc ttaatccttc ttttaatata tttatacttg catttatatc tctatcgtga
     5881 gtttcattac aactaggaca aatccattct cttatattta agttcttaac ttcctcgttt
     5941 ttatacccac atttattgca tatttgtgaa cttgcaaaga atttacctac ctttacaatt
     6001 tgtctaccat accaattagc cttgtattct aattgacgaa taaactctga ccatgataca
     6061 tcagaaatta agcgagatag tttacgattt ctaatcatat tctttacttg taaatcttct
     6121 atgcaaataa tatcgttctc ttttattaat ttagtagata gtttttgtag aaaatcattt
     6181 ctttgattag ctatatgttc ttgaagtctt gcaactttta atcttgcttt atttctattt
     6241 aaactaccga ttgtttttct cgataattct ctttgtaatt tagcaagttt gtttaatgac
     6301 ttcttaagat acttaggatt ttcta
//
LOCUS       DAELTA010000195         6322 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.196, whole genome shotgun sequence.
ACCESSION   DAELTA010000195 DAELTA010000000
VERSION     DAELTA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.196"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..1508
                     /locus_tag="KPG13_003274"
     CDS             189..1508
                     /locus_tag="KPG13_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG0986965.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(1583..2011)
                     /locus_tag="KPG13_003275"
     CDS             complement(1583..2011)
                     /locus_tag="KPG13_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986966.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(2102..3478)
                     /gene="hydG"
                     /locus_tag="KPG13_003276"
     CDS             complement(2102..3478)
                     /gene="hydG"
                     /locus_tag="KPG13_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG0986967.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(3479..4564)
                     /gene="hydE"
                     /locus_tag="KPG13_003277"
     CDS             complement(3479..4564)
                     /gene="hydE"
                     /locus_tag="KPG13_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG0986968.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(4600..5808)
                     /gene="hydF"
                     /locus_tag="KPG13_003278"
     CDS             complement(4600..5808)
                     /gene="hydF"
                     /locus_tag="KPG13_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG0986969.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(5823..6074)
                     /locus_tag="KPG13_003279"
     CDS             complement(5823..6074)
                     /locus_tag="KPG13_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG0986970.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2132 a          973 c          909 g         2308 t
ORIGIN      
        1 gtttatttga taaaaataaa atatattgtt tctgaataac aaatattttg aaaattataa
       61 aaacatatat tgacaatttg tgtcaaaaat actaacatta agtatataaa gaattttata
      121 tgagaatatc tttataaatc gcaataattt taaaacataa atttatttaa agaggggatg
      181 atttgccaat gagcgagata actagtcaaa tgataagtac agaagaaaaa aaatatgttg
      241 ctaaaactca aaagatacct tattatccag tagcatttaa atcaggtgat ggtgcaatgc
      301 tatatgatta tgaaggtaat gaatatgtag attttttagc aagtgcaggc tctgcaaatg
      361 taggtcatgg taataaggaa atctcacaag cagttaaaga acaaatggat gatataacac
      421 aatatacact tgcatatttc catagtgatc ctcctgtaaa attagctgag aagcttgtgg
      481 aaatagcccc tggagataat gataaaaaag tactatacag tgcaactggt tctgcttgta
      541 tagatgctgc tataaaatta gcaagaggat atactggaag aacaaagata atatctatgt
      601 gtgaatctta tcatggaagt acttatggag ctatatctat atctgcttta agtacaaata
      661 tgagaagaaa aatgggtcca ttgttaccag aagtttatca cttccattat cctgataaaa
      721 atagaacagc taaagaatgt ttagatgaga tagaatatgc ttttgcacac tatctaccag
      781 cagaagaggt agctgcaata tttatagagc caattgcagg agatgcagga ataatagtgc
      841 ctccagtaga gtgggtacaa ggtttaagta aaatatgtaa agaaaatgga atattacttg
      901 taagtgatga aatacaacaa ggtatgggaa gaacaggtaa gtggtttggt atagaaaatt
      961 ttggtgttga agcagacctt atagttcttg gaaaatctgt tggaggtgga ttacctctag
     1021 gagcagttgt tggaagaaca gaaataatgc aaagtcttga tgcaccagca catttattta
     1081 ctttagctgg aaacacaaca gtatgtgtgg ctgctttaaa atctatagag ataattgaaa
     1141 aagaaaactt acttcaaaag agtatagaaa tgggagatta tattaaagca ggatttgaaa
     1201 aactaaaaga aaaatacgat ataattggag aaataagagg cataggatta tctataggag
     1261 ttgatatagt taaaggaaaa ggttctaatg aaaaacatcc agatgctaca gctaagatat
     1321 gttatagatg tatacaaact ggattaataa tgatattctt aggacaatct actttaagag
     1381 tgcaaccacc tctagtaata actaaggagc aagtagataa agcaatgaac atcatagatt
     1441 ctgcaataga tgattattta aatggtagaa ttggtgatga agtatatgaa gtaactcaag
     1501 gttggtaaaa taaatattaa atgaaaatag gagttatctc aattttagaa aattatttct
     1561 gaaatgagat aactctttta agttacccac aaataagaaa attgtttgat tcattaaaga
     1621 ctatatttcg gatgtcgttt ttgtgagtat cagagacagt attacgagaa taattacctt
     1681 tattcgtact atctgttaat ttagttataa ttaagatagc taaaagaata agaattactg
     1741 tgtataaagc aataacatat ggttttcttt tatatttgta tttgttattg aaaaaagaat
     1801 ggccataatt atttttattt actttagatg tatttgtatt ctttttcttg ttacctttct
     1861 ttttatcatc cttttttcta tttttagggt aaggaatgac tttgtgtgta ttatcaagtt
     1921 tgatattttt atttttttga atattgtttt ccaggctagc ctttttttct ccaaattgaa
     1981 tagttttatt ggtattgttg ttatctttca atgttaatct ccctccctta aactttccct
     2041 ttagataata ttactactaa cctatggaat atacaataaa taatatataa gtaataaaat
     2101 tttataggta taaatctctt tctccacgct ctaaggcagt catattgtta gatactatgt
     2161 ttcttatatc ttctctttct atcttaccta tttcttctgc gataagtttt ttacccataa
     2221 ttttaagttc ttcatctcca taatcgatta aaaattcatt taatgtagta agagcatttg
     2281 gtgtacatac attatggatt tgtcgacttt tagctaagct catgaatcta tcgccagttc
     2341 tacctttacg ataacaagct gtacaataac taggtatata tccatcagta atcagttctt
     2401 taagcacctc aacagggctt ctatggtctc caacttcaaa ttgttctgta ttgtcaccat
     2461 cttcatatgc tttatatcct ccaacacctg ttagagaacc tgcactaatt tgtgatacac
     2521 catattttag taactcattt ctcatttcag cagtttctct agtggacatt atgattccag
     2581 taaaaggaac agccaatcta gttatagcta ctattttttt gaacatatcg tcagaaacta
     2641 agtgaggaaa atcttctaag ctcatacctt cagctttttt caatctaggg aaagatatag
     2701 tatggaaacc tacaccaaat ttttcctcta agtgttcgtt gtgcatcata agtcctagaa
     2761 cttcaaattt agggtcagat aaaccaaata aaactcctgc accaacatca tcaacaccag
     2821 cttccatagc tctatcaaaa gctgttaaat ggtaataata gtcatttttt atagattgac
     2881 catgcatttt tataaatgtt ggtttgtgat aagtttcttg gaataatata taagttccta
     2941 ttcctttttc ttttagtaat ttatactcat ctacacttgt agctgctatg ttaacgttta
     3001 cccttcttat atttccgttt tcattatatg ttgaataaat tgcatctaag caatctaaga
     3061 tgtaatttat atcacaattt ttagggtctt caccagcttc tagagcaagt cttttatgac
     3121 ccattttttc taatatttta acctcttcct ttatctcttc catggtaagt ttttttcttt
     3181 taaatttgtt gcatttacta aaaccgcaat aaacacattc atttacacag tagtttgata
     3241 cataaagagg ggcaaataaa accactctat taccatatat ttcatttttt atttgcccag
     3301 ctatactaaa taatctatct agttgttttt tatcttctac ttcaagaagt gtagctatat
     3361 ctttataact aagtttttcc cttctatcag ccttatcaag taccttttca atatcatcag
     3421 aagttgaatt ttttgcatct tctaataaag aattaataag ttcatggttt atgaacattt
     3481 acaaacgcct ccattcaata ttatcaccgc gagaatagtc aacttcaagt cctaatgact
     3541 tgatgttttc ttctaaactt tggtgatatt cacaagcttc ttgaccagta aaggctttgt
     3601 tatcgtaaag agaatattgt tttctaaatt cttgaggaga aaggtttggc ataattacat
     3661 tgcaaccagc taaaagacct gcatttctac cactagggtt tatagatgcc aaggctgttg
     3721 tagcgggtaa taaaactttt ggaagtagaa gtcttgtaat tgaaagcata agtaatgtct
     3781 tctctaaatc tccatgttta taatccttaa aaattgtatc atggtgtgga ataaaaggtc
     3841 ctattccaac catatgagga tttaactctt ttaggtataa taaatcacga acaaggtctt
     3901 cgttgttctg aaatggtgat tcaaccataa atccagcacc tacctgataa ccaatttctt
     3961 taaggttttt taggcattct tttctagttc taagctcaat gtttggcgga tgtaattttt
     4021 tataatgact gtctgtagca gtttcatgtc ttaaaagata tctatctgca ccacataaaa
     4081 agtatttttt atacgaatca taactctttt ctcctagtga tagagttatg gcacaatcct
     4141 catatttatt ttttatactt gaaacaattt cacatatttt atcatcagta aaatatgcat
     4201 cttcaccacc ttgcaaaacg aatgttctgt atcctatttt ataacctata tcacaacatt
     4261 caagtatttc atcaagtgaa agcctgtatc ttatgatatt tttattagaa gattgtattc
     4321 cacaatagta gcaattattt ttacaatagt ttgtaagctc tataagtcct ctcaagtaga
     4381 ctctatttcc ataatgttta tttctcatgt cgctagcttg tttataaaga tagtttctta
     4441 aattttgttc gaagttatat tcctcattgt cgatattttc tattaaatat agtagttcat
     4501 caaaacttaa tgaattaatg ctgtaaagtt tatctataaa gtatttaatc ttatttttag
     4561 tcatattcaa atatgtaatt attaagtata gttaaatttt taataattga aaagttcaag
     4621 tgatctttcc aaaatacctt ttacatatgc tattaagaca ccatagttta caataggtac
     4681 attgaaagat tttgcctttt ctattctaga taacattcca gctctattca tcatacaagc
     4741 tccacagtga actacaaggg catatttatt tatatcttca gtgaaggata ctccagaact
     4801 aaattcaaaa gtaatgtttt tgccagtttt ctttctaatc atattaggaa tttttactgt
     4861 tccaatatca tcagtttgtc tatgatgggt acatccttcg gccatcaaga ttttatcacc
     4921 atcttttaaa ttttctaaag cataagctcc attaatcaat tctttaaggt ctcccttttg
     4981 tctagcaaac aatatagaaa aagatgtaag aggaatatct ttttgtgtat ctttatctac
     5041 ttgtggaaat acttgagagt cagtaataac aagttttggt tttttgccta aattagatag
     5101 agtttctttt aaactatctt cttttgtaac tatagaaatt gcaccattat ccaaaatatc
     5161 tcttataact tgctgttgag gaagtataag tctacctttt ggagctgctt tatctattgg
     5221 aacaactaaa acaactaaat catttggttc aattaaatct gataccaatt tgaactcagt
     5281 gctatcctta ggcaaaactt ttattatttc attttttaaa ttttcaattc caattttatc
     5341 tgttgatgag acagaaacaa ctggacattt aactttattt ttagttaagt ttaaaatttc
     5401 tgattgattc ttaatagtat caattttgtt aagaattaga atatgaggga tattcttatc
     5461 attaaatttt tcaattagtg acaaatcttc ttgtgatata ccaatttggc aatccactac
     5521 tagtaaagct atatctgtct tttctaaaac atctagtgat ttacctattc taagttcacc
     5581 aagttcacca acatcatcaa gaccagcagt atctataaga acacaaggtc ctataggaag
     5641 gatttccatt ggtctaaata ctgggtctgt agtagttcca gcaatatctg agactattgc
     5701 agcactttga tttgttatag catttattat gctagattta cctgcatttc ttttaccaaa
     5761 gagacctata tgtactctta ccgattgtgg agtagaattt agactcattt gtactcacca
     5821 ccttaaactt ctttttttga tatactagtt tttacagata catgaggtat gttgcccaat
     5881 ttaccagtca aactattaat agtgtccatt tcgccaacaa ctgctataca tacgattgag
     5941 acaccttctt cttcgcaagg aatgcccatt cttcctttaa taataccttt aaaacctgat
     6001 acaacctcat tgaatttaaa ttgacattct tttggttctt ccaaaatagc acttataact
     6061 gcaacctttt tcataaatat cctcctaact cagaaaatat gtcttaaaca aatccaaaat
     6121 caaaaaactt ttctttgtaa agaaaagttt ctacaaaaga aaagttatta attacacaag
     6181 tataatgtgc tatattaaac tatttcctta cagattagat aactcttttc tggtagggtt
     6241 agcttgtaaa aatttaattt aacaacaagt aactgaagaa aatttttata caatttatat
     6301 aaattataat aaaaattaac ta
//
LOCUS       DAELTA010000196         6296 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.197, whole genome shotgun sequence.
ACCESSION   DAELTA010000196 DAELTA010000000
VERSION     DAELTA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.197"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(1056..1718)
                     /locus_tag="KPG13_003280"
     CDS             complement(1056..1718)
                     /locus_tag="KPG13_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986971.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(1735..4290)
                     /locus_tag="KPG13_003281"
     CDS             complement(1735..4290)
                     /locus_tag="KPG13_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG0986972.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(4530..4718)
                     /locus_tag="KPG13_003282"
     CDS             complement(4530..4718)
                     /locus_tag="KPG13_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0986973.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(4730..4909)
                     /locus_tag="KPG13_003283"
     CDS             complement(4730..4909)
                     /locus_tag="KPG13_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986974.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(4914..5171)
                     /locus_tag="KPG13_003284"
     CDS             complement(4914..5171)
                     /locus_tag="KPG13_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986975.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5431..6296)
                     /locus_tag="KPG13_003285"
                     /pseudo
     CDS             complement(5431..6296)
                     /locus_tag="KPG13_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         2087 a          977 c          635 g         2597 t
ORIGIN      
        1 tagtaattat gagtttaaag attatttgaa ttatataacc taaaaatcag tacacaaatc
       61 tgatataaag gtagatattt cagataaaat tataactttg tatacttgta actatgaatt
      121 taaagatgct cgtactatag ttcatgctaa attaatttca aaaaaagaga ctgataagta
      181 ggtttgtgta aatgcagact tacctacagt cttttttgaa cgtaaaatta gtaagtatgg
      241 aataatattc atattaaact tttgtaggca aagaaatgaa aaatcaaaga gaaataatta
      301 ttacagattt agattaccaa gctgaattaa gaaagtgtaa aactatggag gatgtagttg
      361 gtgaagatag tatataatag tttaacttat aaattatctt ggtgatatta atgaagtttc
      421 aaagtatata ttatttattg atataaataa tattaaatct gttgctaaca gatgtacctt
      481 tgtttggaga caaactattt aagaaatata actaaaatgt tgaagtagct acctgacttt
      541 attttgaaat gcgaacaaga ttttggcatt aagataattt agaaagaatc actggtacac
      601 aaggatgtga acttagaatg aatagaagta ttcaggcata aggagctttc acccaaataa
      661 aacatagtat gaactttaaa agaattctaa tcagaggcaa tcaaaatgtt ctttcatagt
      721 ttatacctgt agtgttagac atagtattta taagtttcac aaataagata atcattaata
      781 aatgtagtat gtatattcat cctctcaaaa atatttttta atcttacact ttgtaaatta
      841 ttctatttta aaatggtatt tttatacctt tatttcttat gaccgttttc aagctcaaat
      901 tttgcataaa tataaattaa tgaaaagcaa gtaaaaaaag aactgtcgca cagcaattta
      961 aaattactca ttcgacagtc cttatttcgt ggatttaaat agtggagaag aatcacaaga
     1021 ataataccca cgattaaacc tttatacaat acattttata tgactttacc atcactaata
     1081 gttattatac aatcagccat cttagctatt tcatcattat gagttatcat aaccaacgtc
     1141 tgcttgtatt catttacaca atactttagt aaattcatca cctcattagt tgttttactg
     1201 tctaaatttc cagtaggttc atctgcaaaa attattgatg gcttattggc aagtgatctt
     1261 gctattgcaa ctctttgttg ttgaccacct gataattcat taggaaattt attaatctga
     1321 ctttctaatc ctaacttagt tattaaatca ttaatataat tattatctat ctttccttta
     1381 gcaattaaaa taggaagaac tatattatca taaacattga taacaggtat tagattatag
     1441 ttttggaata taaacccaaa ttctttactt ctaagattag atagcttttc atcacttaaa
     1501 gtatataaat ctaaattatc cataaataca ttacctaaag ttggtttatc aagccctgcc
     1561 atacagtgaa gtaatgtgct ttttccagaa ccactttgtc caattattgc actaaattta
     1621 tggggttcta tctctaaagt aattccatca acagccttta tttcattttc tccttttcca
     1681 tatatttttt taatatctat aacttttaac atcttcatac atttacctcc caaattattc
     1741 attctctttt ataccttcaa taatattcat actttcaaaa tctctattac ttacaactac
     1801 tgatattaaa catacaaata cagatatagc aaagaatagt aaagattcct taattgggaa
     1861 tgtaaaagac ataaaattct ctattccata gtttatatac gcatcattat tccaatttgt
     1921 aaatttaact agatttataa atgatggaat catagctatt ataaagctaa aaactgcata
     1981 tgctaatgct tcatatataa gcattttttt catattttta agactaacac ctaatgctct
     2041 aagcgttgaa atttcttttt ttctaattag tagatttgtt cttatagtac agaaaatatt
     2101 aattgatgct attattaaaa ctagtgcaat tattgctatt tgtgatttaa tgtaactttt
     2161 atatatactt tctgacattt catgataact taatctacta ttaaatagag accctggtat
     2221 acttcttgaa attttctgta tctcattatt aacaatctca tcatatggat tttctaaatt
     2281 tatgcctaat tgagtgtatt tttgttctcc tgttatgttt ttagagtgaa tattacttgt
     2341 tataatttct agatctggca taaaatcgcc atctcccata gcaatccatt catcctttaa
     2401 aattacagcc actcttacct tcatctcttt ataaacaatt gtattatttt cttcaacagg
     2461 aatttttaca gttattatgt ctcctatatt taattctttg aatataggct ttatagtatg
     2521 atcatcaact atatcataaa aataattata tactgctaca tttggatatc catcagtgtc
     2581 ctttttaata tcatctaagc taccttcttc aacaaagtca tttaactttt catctatata
     2641 atcatcatca taaagcttta aaactaattt atcttccatt atctcttttt tttctactcc
     2701 attataggtt gtaaattctt tgtttatatc cttattatta aaagtaaaca ttccatttgt
     2761 tatttgaatt ttatgtatac ttttaacacc cttaattttt tctatattct ctacatcact
     2821 atctgtgtat tttgatattt gcgggtctgc atttactcca aattgcattg ttatattata
     2881 tctattttgc atatcttcaa ttttactgtt gacctcttct tgcatacttg agaatgtagt
     2941 aatgaatata tatcctccta aagcaataga caacatagat atagcagctc taaccttatt
     3001 tcttccgata tttttaagtc ccataaatcc ataaaatcca aatattttgc ttactagtct
     3061 gtctattcca agtctacttc tgtgattttt aattttgtct gtatttatta ttgattcaat
     3121 aggtgatatc ttgccgattt taaaagttgg tataatacaa gatataaaaa cagaaatcat
     3181 tccaaccaat atagctttta taactatttc attatttata tataatttag ggttaagacc
     3241 agcttcactg tataaaactt cgttataatt actaattcct atatatgaat atgtaacccc
     3301 agctactaac ccaagtagta tacctattat tgttataatt aatgcttgat agattatcat
     3361 acaccttatg tttcttttct ttgcgcccat cagtctaagc agtcccattt cttttactga
     3421 ttgatttagt gtaatattga aaatattaaa tatgaaaatt atagatactg tagttattaa
     3481 aacatttctt ttaaaaacat tcggatcatt cttatcatct tctacataac gaagttccct
     3541 tacaagaggt acatttggta gaaaactttt ttgtcccaac ttatattttt tcataagata
     3601 atcacattca ccttcaatat ttctccatcc acttttaaaa cttaatatac tattataact
     3661 aatcgcatca tgaggtataa tgttatttgt ttcatcattg ccataagtta ctgcttcgta
     3721 ttgccaaacc acatcatagt acccttttgg tttttccata actcctacta ttttaaattc
     3781 tttatttata gaaaataatt gattacttcc gttagtatct ttatagttct tttttattgt
     3841 taaatttaat gtagatccta ctccttcact taaattcatt gcttctagtg cttttttctc
     3901 taatactatt tcattgttgt ttttaggtag tctaccttct attattttgt tgtatctact
     3961 ttttagatac tcttcgtttg atgaagttaa tataaatgag tttccttttt tatctgaaat
     4021 acgtcctaga ttttgtaccg aattaccctt tgatactttt tcatcagttt ctaatttttt
     4081 agcatcatca agtgtcaaat cactaaactc tacatggtat ccactgtaaa gttcatgtat
     4141 tgctactaca ttgttttcat tttgtgaatc ttttataaca ctaggtccaa atatcaacat
     4201 aacacctaac ataactccta ttattaaaag taatgttcta agtttatttt tctttaaata
     4261 tgctagtgac atttttaagt aaatccccat ataatcctcc tattttacta taaattattt
     4321 atccaactca tctgttaagt agattggtgg atattttaaa tcttttaatt cttcattatt
     4381 ttttgaagct tactaccatc ttcctcatga taaacttgca gtatcacctt atttacatgt
     4441 agtgcataat aatcaccatt ataaaaatat tagtatttat aacctggata atttttgtaa
     4501 aatgatttat aaagtatccc atcctaattc tattttgtat acttttcttt tgtaatacgc
     4561 tctatatcat cacctgaaac tccttgatat gtcatatatt tatctatata tttttcagct
     4621 tcattccatt ttctaaaata gttgttgtat ataataaatc ctattatggc tataatgctt
     4681 ataattaaag ttaataaaat agctttgtat tttctcatag tgaatcttcc tacttcaatt
     4741 taaattcttt aataagtttt aattctaaag acttagtttt atcatcaaat ttcttaactt
     4801 gaatttttat atctcccaaa ttatcaacct ctacatttac ttcaccttta tcaaatactt
     4861 cttcaatttt tctaaaaaat tctttctcac ttatttctat atgaatcaat gatctatatg
     4921 ttataaatgg ccattatgaa tttatcatct tctgacagct tttcattata agaaaaatct
     4981 ttaacacatt gaattgataa tatttccttc gtatccttct tttccttagt tattgaataa
     5041 tgtaaaactt ggctaggttg ttcttttgta tactccaagt ttcccaaaga caaaacaaaa
     5101 cttccatctg atttattttc agctacccta agaccactat tttctattga ttctgatatt
     5161 ttctttgaca agtcttcttg atactagaaa tctgttcaat agtatatatt ttttcatttg
     5221 tacctgaaac cttatttgtg catcctgtta ttaaacctag tgttaagctt atagctataa
     5281 ccatagttat tgtaattttt ttatcttcat ataatcctcc ttatatattg aaaactcgtt
     5341 atttctcata aaaaaaagac gctatctttt tctattaaaa gataacatcc ctatatgttt
     5401 agtatcaata tatgttcccc aactggtagg ttaatcgacc caactatcaa aattttgatt
     5461 taatggtata taaatattca atataaattt gtctttttta tcttcaaatg ataaatctcc
     5521 tctatatttt tttatagaag atttgatgct ttaaatccct attccatgta caagtttatc
     5581 ttttttatct gttaataata aacttttatt ctttctaatt gaatttattt tactattctc
     5641 acatttaatt acgaaatagg actttgagat agttcctttt atttgtatat atttatcaat
     5701 acttttatca tgtatcttat cgcaagcttc tattgcatta tctaagtatt tgcaaatata
     5761 ttagatacat ctattggttt tataaaattc actttggaaa aattgatatc acaaatgaat
     5821 ttaatttttc ttttttcact tacatcactt ttttcattta ttattatatc caatataaca
     5881 ttacctgtat tgtaaattga tttaaattct ttaatttaat cttttaaact atttacatac
     5941 ccatcaattt tcctttttta tatttgagat tttctataca atatatatga ttatttatat
     6001 catgataaag cttcttaact ttagcgtgtg attcttgaat acttagataa tgagaatact
     6061 gcatatctaa cttatttttt attacttcat tttgcaattt taacttattt tccttaatta
     6121 tttttataaa cactataata atgactaagc tactagctag tattaaccta ggtatcacaa
     6181 tatacccaac tatatatatt tcctaatacc caccaaatcg atacattttt ctatttatat
     6241 aaattattaa tatagtaaat aaatttgtta ttaaagcaac agataagtat atatag
//
LOCUS       DAELTA010000197         6183 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.198, whole genome shotgun sequence.
ACCESSION   DAELTA010000197 DAELTA010000000
VERSION     DAELTA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.198"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            142..1623
                     /locus_tag="KPG13_003286"
     CDS             142..1623
                     /locus_tag="KPG13_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0986976.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1656..5090
                     /locus_tag="KPG13_003287"
     CDS             1656..5090
                     /locus_tag="KPG13_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG0986977.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5108..5854
                     /locus_tag="KPG13_003288"
     CDS             5108..5854
                     /locus_tag="KPG13_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG0986978.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5803..6111)
                     /locus_tag="KPG13_003289"
     CDS             complement(5803..6111)
                     /locus_tag="KPG13_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG0986979.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         2578 a          609 c          933 g         2063 t
ORIGIN      
        1 tatcttttaa atgataatga tatttattta aatactcttt attaacaatt tataaaactt
       61 aattgtaaaa aatacttatt aatagtaaaa taaagtgaat caattatatt ataattgcta
      121 aagagagtga ggtgtagcta tttgaaaaaa aatagattaa tagcaaagaa tatatcaata
      181 gcatttatag tactattctt ttttagtgta tttacatttt tttatgtagg taatattaat
      241 agagtgttag aatatgaaac aaatgacata ataacagtta caattgctgg atggattata
      301 ctttcatttt tatttttagg tattataatt tacatattat attcaaaagc aaatagtcaa
      361 aaaactatag aaaaagtagc atatacggat tttgtaacag gttattccaa ctggagaaag
      421 tttgaattag atgttacaaa tttattaaag aaaacatcac aaaataataa atatgcaatg
      481 gtcatattcg atattgataa atttaaagca attaatgata tatatggcca taaaaaggga
      541 aatttaatat taaaagatat tgcagatact ttaaatgaat tgactgatat caatgaaact
      601 tttgctcgtg taagtgctga taatttcaat atattattaa catataacaa aaaagaggat
      661 ataataaata ttatcaaaaa gattatggca aataatgaac ttgtaaactt atcttttgga
      721 atttatgaaa tcaaagataa ggatttatct gtaagtgtat atagtgatag agcttcactt
      781 gcaaaatcat caataaaaaa taatagtgat gtaaattttg cattttttaa tgacaaatta
      841 agagagaaat tattatttga agataagata gaaaaagaaa tggaatatgc tcttgaaagt
      901 gggcaattcg taatgtattt acaaccaaaa tacaatatta aattagataa attttgtgga
      961 agtgaagccc tagtcagatg gcaatataca gaaaaagagg tcatatatcc aggtgatttt
     1021 attcctatat ttgaaaaaaa tggatttatt agaaagattg atatgtacat attagaacaa
     1081 gcttgtaaag aaatccggtc tttatttgat aaaggaattt cacctttacc tatatctgta
     1141 aacttctcaa gagttgattt ttttaaaaaa gattttattg aaaacatagt aaatatatgt
     1201 gatagatata aaataccata tagtttaata gaaattgaaa ttactgaatc tagtatgttt
     1261 ggagatactg atactctatt taatgttagt agaaacctac aggatattgg ctttatagtt
     1321 gcaatggatg attttggctc tggatattct tctgtaaata tgcttaaaaa tataccatta
     1381 aatgtaataa aattagatag aggatttttt gttgatgata aagatgttga taagagtcaa
     1441 attgttataa aaagcatagt ttctctcatt aagcaattag gaataagggt tgttgctgaa
     1501 ggaattgaaa caagaagtca aatagaaatg ttaaaaaaag caaattgtga tattgttcaa
     1561 ggatattatt tttcaaaacc attaccaatt aaagaatttg aaaaattagt ctataaaata
     1621 tagtcagcga gtcttaaatg ggaggtggtg attaagtgat aagagcaaaa tcgttaaaaa
     1681 atgtactgag tattgtttta atatttttat ttacaataat ttctttttct gattttgcat
     1741 ttaaaactaa tgtccaggaa gaagaaaata gtaaaattaa tttaagagaa atatccctac
     1801 aaagttccaa tgctataaaa ataaaattag atagttctct atcctttatt aaaagtgtgg
     1861 caaatatggt ttcttatttt aatactatag ataactcaaa aatagttcca ttattagcta
     1921 atatatcaaa agaatctgac tttcaaagaa tctctattgt atctcctgat ggaataagtc
     1981 ataccagtga tggcaaaatt atcaatgtgt caaagtatca gtattttaag aaagctatta
     2041 aaggagaaag taatatatct gaagttgtga aatctccaat tgatggaaaa gatacttttg
     2101 ttatagctac tcctgtattt aataagaata acaaaataat tggtgtttta tacggttctt
     2161 actacaatga taaattagct gaatttatag atataacaag ttttaatagt gaagggtaca
     2221 tagatatatt tgaatctagt ggtcaatttg ttttaaaatc ttcacataca aacaaactga
     2281 ttaagtcaag tagtaacata tatgatatat ttaaaaaagc ttcttttgat gatggttata
     2341 gctataaaaa aatatatgaa gatacaaaaa ataacaagag tggctttacc tcatatgaat
     2401 acaaaggaga aaatcgttat gctacttata cacccattgg tgtaaatgac tggtatgtaa
     2461 tttctgtgat accacaatat actgtaagaa gaaacattga taaaataaat caattagcat
     2521 tttctcttat tttcaaggta ttcttattgt ttattatgct tataatatat attatttata
     2581 aaaacaataa atctcagaaa gatatactaa aagctaacaa agaaatctca tctattacaa
     2641 atagcattcc tggaggagta caaaagtgta ccctgggtga aacatgcaat attaattttt
     2701 taagtgatgg atttatagag ttgtttggtt atacaagaga agaaataaaa acattgttta
     2761 ataatgattt ttatagttta atacataaag atgatgttga aagaataaaa aaagaaattt
     2821 ttaaacaact ttacaaaaat aaaactgttg aattagaata taggattata acaaaaaatg
     2881 gtaatacagt atgggtttta gataaagggg agcttataac agaacctgat ggaaaagaat
     2941 atttttgttg tgtaactgta gatattacta gaacaaaaca attagaacaa gagatggaac
     3001 aaaaaaatct agaacttgaa atgatatcag aaaatgtttt aggtggtata attataacag
     3061 aatttaatga tgattttact ataaaatacg ctaactctgg ttatctttca atgattggat
     3121 atacacgtga acaattagaa aatgaactta aatacaaagc ttgtaatctt gttttttatg
     3181 aggataaaaa gaaggctctt gaaagtatac aacatcaatt aaacacaaaa ggaatagctg
     3241 tatatcaaca tagaataaga agaagaaatg gtaatattat ttgggtttct ataaaaggaa
     3301 ggcttattgt tgatgatggt attgaaaaag gtatttggat tctaaatgat ataacagatt
     3361 taaaaaatgc agaacaaaaa cttaaaataa atgaagaaag atttagaatt gcattaaagc
     3421 aaacatcaaa cactatattt gagtatgata taaaaacaaa gtcactttta aatactagta
     3481 aagagcttga taaatataat atttataaaa ttactaaaaa cgttccatat tctctagtag
     3541 aatcaggagt tattcataaa gattttagtg atgattttat aaacatgtat gaaaatgtaa
     3601 taaatggagc aaaaaaagca agttgtgtag ttaagatgaa acttccagat gaaaattata
     3661 tatggaataa agttaccttg actactattt ttgatgaaga tggtaatcct gtaagagcaa
     3721 ttggtataca ggaagatata agcaaacaaa aggaagcaga gataagatac aatcaagaag
     3781 gtaagtatca ttcagctata ttgtcggaag caatgataac ttatgaaata aatgtatcaa
     3841 aggatacttt tatcaaagga aatgaaagtt ggcaaaaacc atttggaatt aagcctgaca
     3901 ataactattc tcaaatggta aaaactatag caaatagtat tgtacataaa gacaataaag
     3961 aaacattttt aaatacattt ttatgtgaaa acttattgga tgcatataaa aataatgtga
     4021 ctgaaataaa attagaatat cgaagaatga atcaagaaaa taaaatgatt tgggtcattt
     4081 gtacaatgca cttaataaaa gattctatta cgaacgatat aaaagcactt gcttatgtta
     4141 aagatattga caagcaaaaa aatgaagaaa ttagattaaa attaaaagct gaaagagata
     4201 ctcttacagg attatataat aaaggtacta cagaaaaatt gattaatgat ttcttgaact
     4261 ctgaggaagc aagcttagga atgcatgcat ttttaattgt tgatatagat aattttaaat
     4321 ctgtgaatga taatttagga catgtatttg gagataaagt attatttgaa gtttctaaaa
     4381 acctaaaacc tatatttaga aaagatgata tagtaggtag aattggtgga gatgaattta
     4441 tagtattctt aaaaaatatt cagtcttttg agaatatcaa aaagaaagca gaaaaaatat
     4501 gcgatatatt tagaaattct tatacagaaa ataaaaagac atataaaata tctggaacag
     4561 ttggaatttc atttgtacca gaacatggaa ctgtatttga agaactttat aaaaaagcag
     4621 acatagcact ttattatgca aagagcaaag gaaaagataa gtatgcactt tataatgatt
     4681 gtatctacca aaatgaactt gaaaataata taagtgataa taatataaaa ttagcagata
     4741 aatgtgagaa atccaaacca gtacttaaag aaattcttga ttctattgat aattatatct
     4801 atatagtgaa tccaaatact tatgaacttc tttttgtcaa taataaggtg tctcgtttgg
     4861 atttagagat aaaattagga gaaacctgtt ataaatcact attaaaatct gatagaccat
     4921 gtaataattg ccctcttaac ggattagaag aaaataagca ttcaaaattc aaaatagaga
     4981 caaatacatt tggtaaaaat atcagtatag caagttggat taattgcata gatggagaat
     5041 acaactgtct tatagagtgt atagattctt ctttatacaa aaataaataa aaaaggacta
     5101 tgaagatatg ttaaatagta taactttgat acttggtata atatttacaa tttactattt
     5161 ttatctcaaa atagtttttg gaggaatttc atttagtgag attttcttag ttgtaggaat
     5221 tgtactaatt atctatcaaa tatttaagaa aaaaattaaa gaaaagaagg ttttttatag
     5281 ggtgttaaaa ataataatca gcattttttt aatagtattt gtagttactg agagtctaat
     5341 tattttttat cccaaaaata gtttagaatc aaaaagtgat tatctcttaa ttttaggagc
     5401 ttctgtaaaa aagactacac caagtacaac cctgaagggt aggttagaca ctgcattgaa
     5461 atacctaaaa attaatgata attgttatgt ggtagtatct ggtggtaaag gaagtggaga
     5521 aaaaataact gaggcaaaag ctatgaaaga ttatcttata aaaaatggta tagataaaaa
     5581 taggataata gaagaggata aatccacgaa tacttatgag aattttaaat attcaaaatt
     5641 gaagatagaa gaacatagtc aaggaaaact aagcgattta aaagtaaaaa tagtaacaac
     5701 agattttcat gttctcagaa gtaaaatatt agcatataga aatggatata aaaatacgtc
     5761 tttttatgct agtaaatcaa aactttcatt tgtaccaact tactatacaa gagaattttt
     5821 tgcattatgg aaaacaatca tttttgatag ataataaata aaaagactag atataaactc
     5881 atatctagtc ttttctattc ccttaattct aatccatttt ccaactttac ttgttgtaga
     5941 aatttcaaca ttgggattta caagtaattg cttataagaa gttttatatt ttacgattcc
     6001 aaaatataat tttccttcat aatccattat aaatccgaat ggtcttactc ttggtttatt
     6061 atcttcaact gtagcaaaat agaatgttga gttttcttta aaaaaattca ttattttttc
     6121 atgattaaat cctctttgta tcttatacta atataatgaa tataaataaa atattcataa
     6181 tac
//
LOCUS       DAELTA010000198         5954 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.199, whole genome shotgun sequence.
ACCESSION   DAELTA010000198 DAELTA010000000
VERSION     DAELTA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.199"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            126..473
                     /locus_tag="KPG13_003290"
     CDS             126..473
                     /locus_tag="KPG13_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0986980.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(623..1123)
                     /locus_tag="KPG13_003291"
                     /pseudo
     CDS             complement(623..1123)
                     /locus_tag="KPG13_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1150..2655)
                     /locus_tag="KPG13_003292"
     CDS             complement(1150..2655)
                     /locus_tag="KPG13_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0986981.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2854..3708)
                     /locus_tag="KPG13_003293"
     CDS             complement(2854..3708)
                     /locus_tag="KPG13_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0986982.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4093..4374
                     /locus_tag="KPG13_003294"
     CDS             4093..4374
                     /locus_tag="KPG13_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986983.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4524..5372
                     /locus_tag="KPG13_003295"
     CDS             4524..5372
                     /locus_tag="KPG13_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBG0986984.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5424..5945
                     /locus_tag="KPG13_003296"
     CDS             5424..5945
                     /locus_tag="KPG13_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986985.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSNHLVN
                     IEWFEKLLNDIRD"
BASE COUNT         2103 a          838 c          791 g         2222 t
ORIGIN      
        1 aaaatagtat tatacttata tttaaaaata aaagaatttt attcctattg tgtaaagtga
       61 gaatactatt ttcttatatt ggtataataa tagtaaatct aatatagaaa agaggtattt
      121 ctagtatgca tgaaaataaa aaaaatctat tagacggaag cttagctcaa tataatgata
      181 cagcagttcc aaaaatacca gtatttgctg gtaacgatat aacttcagaa gtttattatt
      241 ttaaacctaa tcaagttctt aatgctcata gacaccctaa tggtgaacaa atatttgtgt
      301 tcctaaaagg agaaggtaaa atgaaacttg gtgagcatga atgtgatgta aaaaacggcg
      361 atactgtatt tgtaccaaca ggagagtggc atgaaattac taatggaagc aatgaagaaa
      421 tggtagccgt tcaaattaca aaaattaatg ctggagcaga atacagagga tagatatata
      481 agtacagtta gtactttaat atctatttta ttatataaac tttaatctca aatataaaaa
      541 gagtctttta aaaggctctt tttatatttt tgttaatttt cattataaaa acaagaaaat
      601 atctagctaa aactaatata ttttatttaa tattttttat agcattgttt gcaaactcag
      661 tatttacgat atctttataa ggtgcttcct tatctagttc tttagcttct ttcataacag
      721 tcatcaattt cttcaaacct tcctctttta atacaggatt ttgagaccat gcatctattg
      781 atttatatct atctacaact gttgttaaat cttctaaaga tatatcttca aagaaaggtt
      841 gcatagcctt agctacttct ttgctagatg cttgttgaat ccataattga cctttataaa
      901 cagcatttgt aaatctttga attaaatctt tattgttttt catgtatgat ctcgtagtag
      961 agtatgctgt aaaaggtatt tctccagaat cttttcctat agatgtaact atatatccac
     1021 taccttcttt ttccattgct gttgctgtag gttcaaatgc tgttgtaaag tcaccctctc
     1081 cacctgcaaa agctccagcc attacaccaa attggtaatc agtctattta taacatttaa
     1141 aacatataat taatctcttg ggatattagg gtaaagtatt aaagaaaaat catcagattt
     1201 ttgattaggt tcttttttat agactgcgta atttattaca cttttaagca atatattttt
     1261 tagataaggt tctttttcct ctgtatattc acttaaaatt ttttcaagtt gaggaacaat
     1321 ttgtgtttta actgtatttc tttccttttg catttcaata tctatatttg ttttattaat
     1381 agcttctctt gcattttcta tacgagataa gagtattttt aaacgttccc caaatgtatt
     1441 actgtcataa attccttgtt ctaaaaaatc atgtagtcgt tctttttgtt tttctaaagt
     1501 atccaattct ttatttaaat ttatcaaggt atgttctaaa gtattttcag gtttagagtt
     1561 gtctttttca tcttttaaag atagtctata atttactaac catttattta agacctctaa
     1621 tattttattt tctaatatgt atagtttagt agctttattt ttacaattag tacaaaccat
     1681 atatttctca tatccatttg atgtagtatt agatttggct attaaagtat gattacagta
     1741 tccacatttt acaattccag ccaatggatt tgttatttta ttgtttggag taggggaatt
     1801 aatacgtttt tttcttatat tttgtgcctt attccatatg tcttcactta ttattggttc
     1861 atgctttcct tttacctcaa tttgttcctc aattcctctt gttctactag aatttctttt
     1921 cctctcgact ttattccata ctatatatcc acaatacact ttgtttttta taatatctct
     1981 tatacccttt tcataccaag ttctacctgt tgtggtctta taaccaagtt cgtttaaata
     2041 attggatatt ttacatgcac catctccttt taaatacata tcaaagatta ttttaacaac
     2101 agttgcttta tcttcattta ttacaagtac tctttcttta ttatttaaat atttaatatc
     2161 atacccataa ggaggagctt gagctataaa tttaccctct tcaatacttt tgatttttcc
     2221 tctttgcata cgtctagata ttaattttaa ttccttacga gccataaaag cttcaaattc
     2281 agaatactcc tcatcaaact catcatttaa atcatacgtt tttctaggag taataatttt
     2341 cgttttagat tttttaaatg tatctagtat taacccttgg tcttgcatat taccacgacc
     2401 caaacggtca atatccatca ctaaaacagc atcatagtta ttatttttaa tttcacctag
     2461 taattctagc atcttaggtc tatattctat actttcccca gaaacaagtt cttctttaat
     2521 ttcaatgaca tttaaatttt gttctcttgc tagttttaaa agagttgtct tatgtctact
     2581 tagagtttca aattcacctc tatgttctgc ttcttcatca gctctgcttt tgcgtaaata
     2641 aattgccaca ttcattaaat cacacccctt gaatatatta tattcagtaa cagattgatt
     2701 atatttaaaa cacaaaaata aaccttcaaa atacaagata tattttttag gtttattttt
     2761 atacagtaaa ttttatagta tatttaaaag cttgtaatct aagtaaatat ttatactatt
     2821 aattttatgc tttcttcatt ttttaatttt aaactatttt tttactacag gtatccatac
     2881 ttcaaattta gcattttgag ggtctggatt taagtacagt tcaatatcag gaccattatc
     2941 atattcataa ccagaagtag gtaaccattc tgttacaata cgcttttcta gttcttgtat
     3001 tgcttgtgga cattgtcctt ctccagaaaa tattgcccaa gtaaaagctg gaacagtgta
     3061 ttcttctaat gtattatcta tagattttgt acttgaaaca gatataaaat acttccattc
     3121 ttctgaatta ttacaaatac ttatacctaa tacaccttga agttgactat ccatcatagg
     3181 gattaatttt tgaagcgttc catctaaagc aaccttttgc cacatttggg gaacaatttc
     3241 aaaattcttt tctagttcat tatgaagtgg ctgagataca ccaactatac gaaatgcttc
     3301 tttcttttca attctataat tcatttcttc ggctccttta atactaattt taaaactgat
     3361 tggaggaaag gcttttaaag ttgttatttc tctttttttt ataactgatg gggcaacacc
     3421 atgaatactt ttaaatgcac gattgaatgc tgttggagaa gagtatccat attttaaagc
     3481 cacatcaatt atctttttat catcattttg taattctaca gctgctaaag acatccgtct
     3541 acgacgaata tattcagaaa gtggtacatc tgccatataa gcaaacattc tttgaaaatg
     3601 atatgtagaa caacaagcta tttttgccac ttgttcatac tcaatttctt ttgttatgtt
     3661 ttcttcaata tagttaattg ctttgtttaa tctttcaatc cattccataa aatcagctcc
     3721 ttgtaattta tataatatct attttaaaat agttaatcct ctctttctat gcactaatta
     3781 actagcttaa taaagaatga acatgagaaa gtctgtaatt aactacaaaa atgaacaaat
     3841 agtattgttt ttcacattaa agtatatcac aaattagtat ctaattttta tataagataa
     3901 cagttttaat ttattatgaa atttttataa tattttagct tagacattta attatatata
     3961 acattttatt tatagttaac tatttttgaa gtttaccctt aatcaagcaa cacttagtta
     4021 ttcaattata aataatgatt gagaatagga caatttttaa gatacataaa atataaagag
     4081 gaggggattt aaatgccaaa catagaaaaa gacttagatg gtacaactac tataacatac
     4141 ctcaacgacg atataggtgt tccaacagac gagaatggat ataaaatatc tatgacaata
     4201 ctacatggaa agacaatctg ccatgtagta tctcctgaaa caatcttagg aagaaagttc
     4261 acattagaag aaaaacaaga aaaagataca ataatatatg gagcaataga aaaaataatg
     4321 gtagacagac aattacaaat aaaaaattca aacaaagaag ttgctagtca ttagcacttc
     4381 tttaataagt aaagtaggac aagtattttt tataatatta ttgacttata gtctaattag
     4441 actataatat aaacatagat agtctaatta gactactatt aaaaataaat acaataagaa
     4501 tttaagaaag gataataata ataatgatta aatcattttt aatgttagga cagtccaata
     4561 tggctggaag aggattcata agtgaagtga ctccaattta taatgaaaga atacagatgt
     4621 tacgtaatgg aagatggcaa atgatgacag aacctattaa ttatgacaga ccagtttctg
     4681 gggtaagtct tgctgcatca tttgcagatg catggtgctg tgaaaatcaa gaggatagaa
     4741 ttgggctaat tccttgtgct gagggaggca gttccttaga tgaatggaat atagatggta
     4801 tactttttaa acatgctata agtgaagcta aatttgccat acaaagcagt gaattgacag
     4861 gaattttatg gcatcaggga gaaaatgaca gtaataatgg caactataaa ttttattata
     4921 aaaaattact ttctattatt gaaactctta gaaaagaatt aaatattcca gatataccaa
     4981 ttattattgg tggattggga gacttcttag gaaaagtagg ttttggaaaa agttgtactg
     5041 agtatgtatt tatcaatcaa gagttacaga aatttgcttt tgaacaagat aattgttact
     5101 ttgttacagc aacaggtctt acatctaatc ctgatggtat tcatattgat gcaatatctc
     5161 aaagaaaatt tggattacgt tattttgaag cttttcataa gaaaaagcat attatggaag
     5221 cattagctaa tgaaagtgag ttgataatac cgagtaattc caagacatat acaaaaacag
     5281 aaaaaatata tataaatagt atggacttgg cactaggaaa gatatcatat gatgagtttg
     5341 aatctaaact tattaaactt aatgataatt agataaaata ttcattaata tactaataaa
     5401 tatagattga aagaggtgat tttttgatac cattattaat tttaggatta ttaaaacaaa
     5461 atccaaattc ttatggatat gagttattag cattaatgga aaagaagcat tacaagtaca
     5521 tagtaaattt cactaaaggt tctttttatt ataatttaca gcaacttgaa gaaaaaaagt
     5581 ttattgaaaa agttgaaaat aaacaatcta ataaaaatag agaaactcat aactacataa
     5641 ttactgaact tggatgccag gaatttgaaa aattaatgta taagtatggt tctaaaacag
     5701 agtatataaa tctatcattt tacgcaacac tattatttgc aaatgattat aaaaatgaaa
     5761 agttaataga attattggaa acgcaaatta agcaaactca agtaagaatt tctctattaa
     5821 atgaatccat agaaaatgac aaaacaaatt cacattattt taatagaatg ctagaaaatt
     5881 ctcgttctaa ccatttagtt aatattgaat ggtttgagaa gttgctaaat gatattagag
     5941 attgatttta ataa
//
LOCUS       DAELTA010000199         5826 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.200, whole genome shotgun sequence.
ACCESSION   DAELTA010000199 DAELTA010000000
VERSION     DAELTA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.200"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(759..1310)
                     /locus_tag="KPG13_003297"
     CDS             complement(759..1310)
                     /locus_tag="KPG13_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0986986.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1501..2463
                     /locus_tag="KPG13_003298"
     CDS             1501..2463
                     /locus_tag="KPG13_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG0986987.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2758..4602
                     /locus_tag="KPG13_003299"
     CDS             2758..4602
                     /locus_tag="KPG13_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG0986988.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4924..5508
                     /locus_tag="KPG13_003300"
     CDS             4924..5508
                     /locus_tag="KPG13_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG0986989.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2270 a          575 c          904 g         2077 t
ORIGIN      
        1 aaattataac tttattttat tagcaatctt ttttatattg ctgttaattc aaaatggagt
       61 tttaaaaaca ggttatcaaa atagagctac attttatcaa gataattatt ttaatagaga
      121 ggataaaaat gataagataa aatttatgac tataaagatt tttatatttt tgtcattacc
      181 attatgtttt aatatactat tcaattattt aacctcttaa attatattta gatataggag
      241 tcttatgata aaagtaagct tatgtgttat ttgtagtaaa tataaagtgc atatagtgat
      301 aaaacaaaag tttcatataa atgaagaaaa aaatataaaa taatttattt aactacttaa
      361 aaataaacta aaagggataa taaaatagct tttaggttat ggtaataaag gtatttaaaa
      421 agagtgcata agcactccaa ttgtgagaaa tacatacaca aatcattatt acaacaatga
      481 aaaatgtaca tataagagac gttttgtatt tgttttaagt gataatattt taatactaac
      541 taaataatta tcacaatagt aaaaaagaaa aaggagtatt ttgtactcca tttgtcaaaa
      601 atataaaact tttatataag cttattataa cataattgat aaaagtgtaa agatatataa
      661 tgaatttata aagaaaaata aaataaaaaa gataaatcct aatcaaagga tttatctttt
      721 tgtatagaaa ataattattc tattattaaa tggcatggct aaactggata ataatttatt
      781 attactgctt tcaatgtttt ttctccttga ttatggtatg tatgaattga ttcagctgaa
      841 aaacaaatag agtcattttc tctaagttga taaatagagt catttacttc taattttaat
      901 tgaccttcta taatcataat atattcctgt gatttttcag aatgaccaac agatgtataa
      961 ctatgacctt cttctaattc catttgaaat aattcgaagt ttctagttgg tgtattggga
     1021 taataacaat aaagacgata atctttattt tctgaaactt gtacatctat atcagttttt
     1081 gatacaataa aagtttcatt ttgaggttgt tccagtattg cagtatatgg aacatttagt
     1141 ccacttgcaa ttttccaaat tgtgtttact gttgggtttg agtctccctt ttcaatttgc
     1201 gaaagcatta ctttacttac gccagataat tcagcaagtt gacctagact taagttacgt
     1261 tcattacgta atctatttaa atttttagca ataacagtat taatactcat agtagctcct
     1321 taaaatgtaa attattgaca aatataattt acgaatgtat aatttattta acattattta
     1381 taatttacaa atgtataata taatgaacgt taaatacact gtacatccgt aatataaatt
     1441 atactgcata aagagttgtt tttcaacgga tgatagttga gagattaagg gggaattgta
     1501 atgcgtgtac ttaataatgt tacagaggct ataggcaata ctcatatgtt acatttatcc
     1561 aaaatagcag atgaatttgg agttcaaggt aatatttatg caaagatgga atacttaaat
     1621 ccgggattta gtaaaaagga ccgtgtagca ttacaaatga ttaccgaagc agaaaatgca
     1681 ggtttattag ttccaggtca agcagttgtg gaattaacaa gtggtaatac tggaacagga
     1741 ctagcaatag cttgtgcatg taaaggatat aaatttatag cgtgtatgtc aaaaggaaat
     1801 tctatggaaa gagcaagaat gatgaaagct ttaggtgctg aagtagttct tgtagaccaa
     1861 atgcctaatt cagttcatgg acaggtatct ggtgaagatt tggcacttgt agaaattgag
     1921 gctcaaaaaa tagcaaaaga aagaaatgca tttagagcag accaatttaa tttagaagct
     1981 tgtaatcatg cacatgaaaa gtatacagca gaagaaatgt gggaacaatt ggatggaaat
     2041 attgatgtat ttgtagattt tcttggaagt ggtagtactt ttgcaggatg ttcaaaaaca
     2101 cttaaaaaac ataattcttc tatcaaatgt tatgttgttg aacctgaaac agcagcaatt
     2161 tattcaggta aagaaataac tgaccaaggg cataaaatac aaggtggagg atattctatg
     2221 gatttgccat tagtagacaa gaaacttatt gatggttata ttcaagttac agacgaggag
     2281 gcaactgatg tagcaagaaa attagctaaa ttagaaggtg tatttgcagg attttcttca
     2341 ggggcaaatg tatgtgcagc aattaagttg ttaaagagca gtgaaaaagg aaaaaatata
     2401 gttttaactc ttaatgatag tggtttaaaa tatttaagta ctgatttata tgaagaattg
     2461 taataatagc acaatagtta tgcaatgttt tttaaattaa gtatatagat ttaaattata
     2521 aaaagtatct caatgtaatt tttgaaagtt ataaaggaga tactttttta ttttgtaaat
     2581 atggagtatc ctacttataa gtattaagct atatagatat tgatgattag ctatgcaata
     2641 aaaaagagta tgtgtttttg catttataca tcataacttg gaataataca tattaatgtt
     2701 ctaaatgtat ttaaaacata tgataaaatt agaacaaata gatacaagga gataaaaatg
     2761 caaaagagaa ttttaagaaa ttttatattt acgggtaaaa atattttaag taaacttgtt
     2821 tttataataa aatataattt tagtacatta attttatttg aattatttca taaaggatta
     2881 gccttatttt ttatatggcc atctattaga tatatatttg atattttaat aaaaagttta
     2941 catatagtct acctaaatat ggataatttt ataaaagttt tgaccaatcc catgtcaata
     3001 gttctgataa tactatcagt tataatacta gctttttatg cattttttga atttacctct
     3061 gtaattattt gttttaataa atcgataaaa tatgaaaaaa tagggttatt tgaactcttt
     3121 aaaatatcat ttaagaactc tataaaattg ttatatccta aaaatatact attatttata
     3181 tttgtgatat taataattcc attagtaaat acagttttaa tttcagggtt tataggaaaa
     3241 atacaaatac cagagtacat actagattat ataaagtctg atataatttt aaatataatc
     3301 tatatgattt ttcttttaat aatatatatt tcggttatta gatggatttt tagcattcat
     3361 gagataactt taaatacaga aaattttaaa agagcaagaa aagaaagtgt taatcttata
     3421 aagggaaaga ttataagaac aattatttat tcattaatat tatctttagt agtagctatt
     3481 attggatatg cgatttatca tagtggaatt atatttatag gcatatggac taaatattgt
     3541 actgatatgg aatttttgaa gaatgtattt ataaatagat gtattgcatt tgaagaatat
     3601 tttagattga ttttatcaat attcattttt atattggaca ttggatttat ctcagcaact
     3661 tattatcaat ataaaggagt aaatatttct aaaaataata ttaaaaaaga gaaaaaatct
     3721 atatcaaaaa ttgtttttaa tttagtatta atattattga tttcttatat agagtcagtt
     3781 gcattttctt cacatataaa tggtatgttt aatacgtcat tttcatttta tacaacagca
     3841 attgctcata gagcaggagc tgaaatggct ccagagaata ctttggcagc aatagaagaa
     3901 gcttcacatg ctaaagctga ttatgcagaa atagatgttc aagaaactaa agatggagaa
     3961 cttattgtac ttcatgattc aaactttaag agaactacag gagtagataa aaatgtttgg
     4021 gatgtaaatt ataatgaagt aaagacatat gatgcgggaa gcttttatta ttatggaaaa
     4081 ggttattatg atgaaaagtt tgttggggaa aagataccaa cactcaaaga aatgataaaa
     4141 tattctaaag gtaaggtgaa attattaata gaaataaaac ttaatggaca tgaaaaggga
     4201 gacgtagtaa aaaaagttgt tcagttaatt aaagaaaata attttgaaaa tcaatgtata
     4261 gttgcatcta tggataaaac tatattacaa aaagtaaaaa aactggatga gaatttattg
     4321 acatgctatt ttatggcaat tgcatatggt gatttttcaa agttaaatta tgttgatata
     4381 tatggaattg aatcaacttt tgttaataaa acagtagtag aaaagattca taaaatgaac
     4441 aaaaaaatct tcgtttggac tgttaatagt gacagtttaa ttgagaaaat gcttgaccta
     4501 aatgtagata gtattattac agataatcca taccttatag agagtgcaat ttattggaag
     4561 aaaaatggat ttattagtca agtgtcagat tatttatttt aaatattcta taattgtatt
     4621 atccagtaaa agagatatta ttaaaatcaa taatatctct ttttaacata aataaggttg
     4681 ttaaattaag gttattctta taaaaattgt gcttatcagc tataaacaag gaataaaagt
     4741 gaaaaaattg ctctgagatt attacagtta aaagagtttt aagatgacag agagatagat
     4801 tgtcaactgt atatttaata aatcttgtta tttattaaat tttatataag taaaatatat
     4861 aaattaataa atcttatgct agaatatata tgaaaaacaa tataaatttg gaggattaaa
     4921 aacatgacag agcgagaaaa aatgttggca ggagaacttt acgattgtgg tgatattgag
     4981 ttgttaactc aatggcataa agcaaaaggc ctagtaagag attataacca aacagattcc
     5041 tcggatttgg aaaaaaaaga aaaaatatta aatgagttac ttggtggtaa gggtaaaaac
     5101 ttatggatta ctgcaccgta ttatgtagat tatggaaata atatttattt tggaaataat
     5161 tgtgaagtca atatgaattg tacgttttta gatgataata aaattatcat tggggataat
     5221 gcactaatcg caccaaacgt acaaatatat actgcatttc atccaactaa tgctggtgat
     5281 agatttggta atgccaaaga agatggttca tttgaatttt gtaaaactca aacagctcca
     5341 gttactattg gagataatgt ttggattggc gggggtgcta ttatcatgcc aggtgttact
     5401 attggagata atgtagttat aggtgcagga agtattgtta caaaagatat tccaagtaat
     5461 atgattgcat acggaaatcc atgccgtatt attcgagaaa ataaataaag ttaaattttc
     5521 ttgcaaaatc tcaaaaaaca cctttcttac tttatacata gggtattttc taatactttc
     5581 acatccatat tcatttgtta aatttttgtg gaatgtagtt tttatcactt ctatcttaca
     5641 tttccaccat ttttttataa aagtcattcc tttatagatt tatttataat agtatatatt
     5701 ctatttgatg tttgaataag cacttatttt taagtaaaat actaactttt aggttagtat
     5761 tttgctatag tatgatgatt aagtaagaat aaaatactat aatacataaa gcttaataaa
     5821 tataat
//
LOCUS       DAELTA010000200         5802 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.201, whole genome shotgun sequence.
ACCESSION   DAELTA010000200 DAELTA010000000
VERSION     DAELTA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.201"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..254
                     /locus_tag="KPG13_003301"
     CDS             <1..254
                     /locus_tag="KPG13_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG0986990.1"
                     /translation="GILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDNNYEE
                     IENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI"
     gene            486..1697
                     /locus_tag="KPG13_003302"
     CDS             486..1697
                     /locus_tag="KPG13_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG0986991.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1890..2150
                     /locus_tag="KPG13_003303"
     CDS             1890..2150
                     /locus_tag="KPG13_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG0986992.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2504..4216
                     /gene="ptsP"
                     /locus_tag="KPG13_003304"
     CDS             2504..4216
                     /gene="ptsP"
                     /locus_tag="KPG13_003304"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG0986993.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(5008..5673)
                     /locus_tag="KPG13_003305"
     CDS             complement(5008..5673)
                     /locus_tag="KPG13_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986994.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         2341 a          565 c          950 g         1946 t
ORIGIN      
        1 ttggaatttt aaattccagt gtttatgata aatactttaa aataactgct aaaaaaatga
       61 gtaaaaatat atatgattac tatcctaata aggttatgaa gataaggatt tttagagata
      121 ataattatga agagatagag aatttatcaa agcaaataat atcaatttta ttaaataagt
      181 ctatagataa aggaaaagta gaaaaattgc agataaaaat ggataacttg ataatggatt
      241 ctttaggtat ataagcagtt gtaagtatta aaaaattata atcttttaat tgtttattta
      301 aatttagaaa taaaaagtta gccatataaa aaatattaaa aatatatact tttcaatatt
      361 tttttgataa aataagaatg aatttgtgca attttatttt tctccaataa ctaaaaatag
      421 ttttttgaat taatatttgc ttaagaataa aaatcagaaa cgtttttgat atagtaggtg
      481 ataatatgaa aggattagtt ttagaaggtg gaggaacaaa aggagcctac caaataggtg
      541 catataaagc tttaagagag cttggtatag agtttcaagg agtaactggc acatcaattg
      601 gtgctttaaa tggagcatat atagttcaaa atgatattga agtaatggaa gatatttggt
      661 taaattatga ctatactcat tttatgaata ttgatagaga aacctatgag aggtttaaaa
      721 atgtagattt cactactaaa aatctcaatg cagtgtttga gcttataaat aaagctagaa
      781 aaaatgaagg tatagatata agcccattaa gacaactaat ggagacaaca ttaaatgaag
      841 acataataag gaaatcaaaa aaagactttg gacttgttac ggtctggtgg gataaaaaaa
      901 ttaatccata tccattgtat atcgaagata tacctaaagg aagattgatt gattatttaa
      961 ttgctagttc cagtttgcct atttttcaaa cgaacatgat ggatgataag ttgtatcttg
     1021 atggaatgtt ttgtgataac atgccaatag gactcttgga agacaaaggt tatgaagatg
     1081 ttgttgtaat taggttgatt gatgatttta ttgggaaaat gaatctaaat cgacatcaaa
     1141 atataaatat aaaaactata ataccatcag aatatctagg aggttctctt aataaagata
     1201 gagataatgt agaaaaaaac atccgactag gttacttaga tactatgaaa gcatatgaaa
     1261 gatgcactgg aattaagtat tattttgatg tagagtataa atataatgaa gaatattgtt
     1321 ttgataaatt tagaagtatg agcaatgaaa caatacaata tatctgtaat ttattgaata
     1381 taaaaagaga tgcttcactt agaaccgtta tggaaagtgt aataccaaag cttggtgagg
     1441 tgcttggatt gccaaaggat ttttcatata aagatttgtt ttactgtata tatgaaaaga
     1501 aacttgaaga aaataatata aacaggataa aattatatga ttttaacaag gttatagatg
     1561 ttgtaaatac aaatattgat tctaatagaa atttagcttc taattttgaa gttagaccta
     1621 aaattagtat taaaaagaac aaaaacacaa aattattgac aaattgtatt ataaaagaca
     1681 taaaaaatca aagttaaata aaaaacaaaa aaaaatttgt aattatactc gaaaaaagtg
     1741 tatattagta gtataatatt tttgtagcac tagtgggaac aaaaaatgac cataggacaa
     1801 aaaagtacca cttagtacca ttaaagtaaa aaacaggaaa acaattttaa aaaatataaa
     1861 atgtaaagtt tataggagga tttaaaaaaa tggaaaaagt agttagtatc aaaaatgcaa
     1921 gtggattaca tgctagacca gcaggaatgt ttgttaagaa ggcagctgaa tttaaatcaa
     1981 cagtagaggt tgtagcaaaa gacaaaactg taaatgcaaa atcaataatg ggaataatga
     2041 gtttaggttt agctcaagga gaagaattaa agttagttgt aaatggagaa gatgaagaag
     2101 tagctttagc tgcaatggtt gaattagttg aaagtggatt tggagaatag atataataat
     2161 tttttaaaaa tttgatattt agaatctaat attacttaaa gtaaagtaac agagaagtat
     2221 taaacatata cagaccaaca tttgtagata tttaatttct ttgttacttt catgtttatt
     2281 taatcagaag attaagacta ttgttaattg ggtataaaaa tataaatatt ttagaaaatg
     2341 aaataagata tttttaatca taaattcaga gtaaaaatac agtaacaaat agtttataaa
     2401 attttataaa aaattataag taaataaaac tataaaaatg taaattattt atataaattt
     2461 ataaagttta tgtgctagat gatatttttt aggaggtagg gttatggctt acaaaggaat
     2521 aggtgcttct cctggtgttg ctttagggaa agcgttagta gttgaacata gtgaattagt
     2581 aattgaaaaa aaatctatag ataatgttga agctgaaatt gctaaattag aagatgcagt
     2641 tgcagtttct aaagaagaat tagtaaaagt taaggaaaag gcttctgaag aattaggagc
     2701 agaggaagca gaaatatttg aagcacattt attagtttta gaagacccag agttaattgg
     2761 ttctgcaata gataaaatta aaactgaaag tgtaaatgca gagtatgctt taaatgaaat
     2821 aaaagagatg tttgttagca tgtttgagtc tatggacaat gagtacatga aagaaagagc
     2881 ggcagatata aaagatgtta caaatagaat acttagacat atattaggaa ttaaagttgt
     2941 tgatttatct gctttaagcg aagaagttgt attaatagca catgatttaa ctccatcaga
     3001 cacagcaact atgaataaaa aaatggtttt aggattttta actgatatag gtggaagaac
     3061 ttctcatact gctatcatgt ctagaacttt agaaatagct gcaatcgttg gattaaatga
     3121 tataacttca aaagttaagg atggagattt tgtggttttc aatggagaca caggtgaagt
     3181 aatcgtgaat cctgatgaag agactataaa taaatatact gaattaaaag ctaagtatga
     3241 agatgagaga aaagcattac agctattaaa aggaaaacca tctgtaactt tagacggaaa
     3301 acatgtggaa ttagctggta acataggaac acctaacgat atagaaggtc taataaagaa
     3361 tgatgctgaa ggtgttggtc tttatagaac agaattttta tacatggata gagattcttt
     3421 cccaacagaa gaaatacaat atgaagcata taaagctgta ttagaaggaa tggatggaaa
     3481 gcctatagtt ataagaacat tggatatagg tggagataaa gaattaagtt atttaagtat
     3541 ggaacctgaa atgaatccat tcttaggtta cagagcaata agactttgcc tagatagaaa
     3601 agatatattt aagactcaat tgagagcttt atatagagct agtgttcatg gaagattaag
     3661 aatcatgttc ccaatgattt catctttaga agagttgtta caagctaaag aagttgtaaa
     3721 agaagtttta gcagaacttg attctgaagg tgtggcttat gctaaagatg tagaaattgg
     3781 tatgatgata gaagttccat ctgctgctgt gatatctgat gtattggcta aacacgtaga
     3841 tttcttctct ataggaacta atgatttaat tcaatacact tgtgcagttg atagaatgaa
     3901 tcaaaagata agctatcttt acaatcaatt taatccagct gttttaagac ttataaagac
     3961 tgttatagac aatgcacata aagaaggcaa atgggcaggt atgtgtggag aatctgctgg
     4021 agaccaaaaa atgataccaa tacttttagg tatgggatta gacgagttct ctatgtctcc
     4081 aatatcaata cttcctgcta gaaagttaat aacttcagtt aaagagtcag atatgaaaaa
     4141 attagctgat gatgtactta atatgggtac tgctgaagaa ataaaaagct atatagaaaa
     4201 gacttttaat atataattaa tgttatatag ttactaaaat tcatttttct aaataacaaa
     4261 attatatttt acgaattaaa aaggagatgt ttcaaaataa aaatttgaag catctctttt
     4321 ttataaaaga ttactaagaa ttacttaatc tttgtaatag tcattttgtg aaaaagcaaa
     4381 actgcattgt ggctatagta tatatggatg ttattatcaa taaaagtact attaaaatga
     4441 agattcattc tcaaatgata tcaaaagtag ttgtgatgga gtaagataat attttgtgat
     4501 ttaatagtaa aaattaaaaa aatatggtat tgaaaaataa ttaaaagtat ttaataatag
     4561 aattagggaa gaataagttt ataagtaaaa ataattgatt actagttaat aaaataaatg
     4621 tgatatccaa ataaatccta aatctaattt gagttttaat atagatagtc taaagtttga
     4681 ttattaaaga tttaattgta agttgttaca gttgatgata ttgattagag attaaagttt
     4741 agagtagaaa gttaccttaa atttaaaaat gcaagataac ccaatctaaa aattataatc
     4801 actcaatagg tttaaggatt tatttgttta ttataaatag gagggtgtct caaaatgaac
     4861 tttttagttt atgaggcact ctcttttata tagaattaaa tatattttat tatttcaaca
     4921 atgaaaaaga agcttatctc tattttgaga cagcctcttt gtatactatt attaatttaa
     4981 tcaataacat aatttaaaac tatattatta gttaagattt cctgctttat tagtaaataa
     5041 ttctttaata gcattgtttc tggaaactaa atcgttatca gagttgtttg tttttttcat
     5101 aatcactgaa taacgttttt tatttataaa aaatattaaa atcttatcat catcaaaatt
     5161 gtattcttta tatctgtcaa tagactcata acctagattt agaatgtttt tgtcttttat
     5221 actatctaca tctaaaatat atgatgggtc agcctttagt aatatattaa ttaaacattt
     5281 ttcttcacca gcacagaata actccatctt acttgatata gatttagatt gctctgaagt
     5341 tatttttgca ggaataaggt tgtcattgtc atctcttaga agataaccat gtgtgtcttg
     5401 ttgatatact aaattaacct ttttcttagg aacactaatc attttatcta aatgtacata
     5461 atgatgattt tcaacaaaac cttcatttaa tatttcaaca aagttattaa ttttacctag
     5521 gcaaaaagat tctatagatt ctttacagaa tttatttgta aatggtatta tgtttacgtt
     5581 ttcctcgtct gtattccatg ctatagcata atccaacgaa tatacttctt cctctatatc
     5641 tgcaaaatta tattgtacta tgtttccttt caaaattact cgacctccat cttataatat
     5701 atttaatata ctttataaaa cttatattat atttaaataa tatcataaat tgaataaata
     5761 tgatatagta aataagaaaa aatttgaaaa aaatcactat aa
//
LOCUS       DAELTA010000201         5726 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.202, whole genome shotgun sequence.
ACCESSION   DAELTA010000201 DAELTA010000000
VERSION     DAELTA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.202"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(220..2052)
                     /locus_tag="KPG13_003306"
     CDS             complement(220..2052)
                     /locus_tag="KPG13_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986995.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2045..4291)
                     /locus_tag="KPG13_003307"
     CDS             complement(2045..4291)
                     /locus_tag="KPG13_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0986996.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4359..4820)
                     /locus_tag="KPG13_003308"
     CDS             complement(4359..4820)
                     /locus_tag="KPG13_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG0986997.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5125..5691)
                     /locus_tag="KPG13_003309"
     CDS             complement(5125..5691)
                     /locus_tag="KPG13_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG0986998.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1814 a         1071 c          632 g         2209 t
ORIGIN      
        1 ttccttacta catattataa taaaataaac actttgtaaa gacataaaat tatgtggcta
       61 tctcaaaata tagagaatcc tctttttcat tgttaaaatg ataaaaatac actttatttc
      121 atacacaaaa gagtgcctta tgaattaaaa agttcatttt aaggcaccct catttatttt
      181 atcttattaa ttacatgtct atgctaagat agttttttgt tacattgctt catcttcttc
      241 aaattgagaa ttgtaaagat tggcatagaa tccgcctttc tttagaagtt cctcgtgatt
      301 tccttgttca actatatctc cttcattcat tacaagaatc atatcagcat cacgtatagt
      361 tgataatctg tgggctataa caaaacttgt tctaccttcc ataagattat ccatagcttt
      421 ttgaatcaat acttctgttc tagtatcgac agaacttgta gcttcatcta atattaaaat
      481 tttaggatca gctaaaatag cacgagctat agtcaataat tgtttttgac cttgagaaac
      541 attacttgct tcttcattta atttcatctt atatccatct ggtaatgtct taataaatct
      601 atgaacatgt gcagatttag cagcctctat aacttcttca tctgtagcat tcaacttacc
      661 atatcttata ttttccataa ttgaaccact aaataaccat gtatcttgaa gaaccatacc
      721 aaacatttct ctaagctcac tacgattaaa atctttaata ttatgaccat caatcagtat
      781 tgaaccgcta ttaacatcat aaaatctcat taacagttta actattgtag ttttacctgc
      841 tcctgtaggt ccaacaatag caactttttg accaggttta acatttactg agaaatcatt
      901 tattatagtc ttgtttgggt tatatccaaa gttgacattt tcaaaatcaa ttttaccttc
      961 taaaccatct attgaaactg cattttcaac aatttggact tcttcttcct cttctaaaaa
     1021 ctcaaatact ctttcagaag ctgctgctgt tgattgcaat aaattggcaa cctgtgcaat
     1081 ctgagtcatt ggctgtgtaa agtttcttac atattgtata aatgactgta tgtcaccaac
     1141 ctcaattgtt ttcttaattg caagccaacc tcctaagatt gataccatta catatcccaa
     1201 gtttccaata aacatcataa gtggttgcat tatacctgat aaaaattgag atttccacgc
     1261 tgaattatac aggctatcat ttagtttatc aaactcttca attacttcat cctcacgatt
     1321 aaatgctttt actattgttt ggccactgta agtttcctca acttgaccat ttacatttcc
     1381 taaatattct tgttgatatc taaagtattt ttgtgatttc tttattattc ttgaaattac
     1441 aaacattgat attggtaata ttaaaactgc cactaatgtc ataattccac ttatagataa
     1501 catcattatc aaaacaccta tcatagttgt tactgatgtt ataagctgtg tcatactttg
     1561 atttaaactt tgatttaaag tatctatatc attcgtaatt ctcgataata tttcaccatg
     1621 cgtcttagta tcaaaatact tcattggcaa tctatccaat tttgctgaaa tctcttttct
     1681 taaattatag gatacttttt gagaaattcc actcattata aatccttgta taaaagagaa
     1741 cagtgcactt atcaaataaa ggaaaagtaa aaaggttaag atttttccaa ttgcattaaa
     1801 atctatacct acgtttcctc cagaaacttt actcactagc ccttcaaata tctttgtagt
     1861 tgcctttcct aaaatttttg gacctactat agaaaacgac gcactaccaa tagcaaatac
     1921 aattactaga attattgata ttttaaattt agataaatat gcaattagtt ttcgcatagt
     1981 ccctttaaag tcatttgctt tttgtcctgc tcccatagat tttcccatag gacctcttcc
     2041 cattctactc attagctagc tcctcctttg aaagttgtga taaagctatt tgctcataaa
     2101 cttcgcaatt ttttagaagc tctttatgtg ttcctattcc aactatttcg ccctcattta
     2161 aaacaattat ctgttctgca tttaagatag tactaattct ttgtgcaact attagaactg
     2221 tactttcagc agtttttgat tttaaagcct tacgaagagc tgcatcagtt tttaagtcaa
     2281 gtgctgaaaa actatcatca aatatataaa cttctggatt ttttgctatt gctcttgcaa
     2341 tagaaagtct ttgtttttga cctccagata catttgttcc accttgagaa atttcagttt
     2401 taaaagcttc cggtttagaa tctataaatt ctgtagcttg agctatttca gctgctgttc
     2461 tcatttcttc atctgttgcg tcttttctac catattttaa gttggattct atagtaccag
     2521 aaaacaatac tcccttttga ggtacatatc caattttttc ccttaaatca tgttgagaaa
     2581 cattttttat attaactcca ccaagtaata tttctccttc agtaacatca aagaaacgag
     2641 gaattaaatt tataagagtt gacttaccac taccagtact tcctataaag gcagttgttt
     2701 ctcctggctt tgctgtaaaa gatatatttg ttaatatagg ttcttctgcg tctggataca
     2761 tgaacgaaac atttttaaat tcaacaagac cctttttatc ttccttaaac tcttgtgcct
     2821 ctgatttttc tgggtctttt atgcttatat ctgacactaa tacttcatct atacgtgtag
     2881 ctgaaacgga tgctcttgga agcataatag ataccattga tatcattaag aatgacatta
     2941 ttatttgcat tgtatattgt ataaatgcca tcatatctcc aacttgcatt gctccagaat
     3001 caacactgta tgcacctctc caaactataa gaactgtgat aaggttcatt ataagcatca
     3061 tagcaggcat catacatgtc ataactctat ttacaaaaag atttgtttta gttaaatttt
     3121 tatttgcttt atcaaatctt tcttcttcat gtttttcagt actaaaagct ctgataactg
     3181 gaataccagt aattatttca cggcttacta agtttaactt atcaacaagc ttttgaacca
     3241 gtttaaactt aggcataacc actgagaata acactacaat taatgataag atagcaacta
     3301 ctgctactgc tataatccag gccattgaag tatttgtgtt taaaaccttt atcactccac
     3361 ctatagccat tataggtgca tagaatacta ttctaagcaa cataaccatc aacatttgta
     3421 tctgttgaat atcatttgta ctacgagtaa ttaatgaagc tgtagaaaat tcattcattt
     3481 cagtatttga aaatgacata acttttctaa atactccact tcttaaattt ctacctacac
     3541 ttgcggctac tcttgctgct aaaaatccta ctgttattgt agctaccata ctaatcattg
     3601 ctataccaag cattttagca ccagtaaata atatataatt attttgagtt ttttctgtat
     3661 caatacctat tttctcatat tctgacctta catatgaaac agagctttgt gttatcatac
     3721 tctctggcat actttcaaag cctttgctca tctctttagt caatgtatct aactgttcct
     3781 ttggcattgc tgataacaat ttaaatatat caacatctcc actttgagta gcttgtggtg
     3841 gaagtttgct cattaactga gctttcatag cttttacctc tttactatcg ccctcaagcc
     3901 ctgatactat taaaatagct ttaccaaaaa taacatttaa atcatctata gtgtctttgt
     3961 ctttagtatt taactcataa agagattcat cttttagacc tggatactta tctttatact
     4021 taacataatc ctttttattc agcaaagtat aattatctag tactttatcc ttactacttt
     4081 tatccataaa taaagtaatt ttatttaatt cactctctct aatcacagat ggaacagagt
     4141 tttctactcc accttgttgt atacctacat ttactatatt ggatgtgtat tctggtaaag
     4201 aaaggtcaca taccgcttga attaccaata gacagactat tattactatt gataatgccg
     4261 atttctttaa atatttaatt aatttaaaca tacatttctt cccttctgta atattaatta
     4321 gtctaataac attaaattga aaaacattat tttaaacttt aaaaatattt ttcatcttca
     4381 tgcttatgac atcctgcttt taaattatct cgtacttgca ttagaagtct tctaagtact
     4441 attttttctt cttcactaaa tcctaaaaaa aactctcttt ccaattcctt gttaaattct
     4501 tcaactttat tacatatttc taaacctttg tctgtcaaaa aaatcctaga tactctttgg
     4561 tccttttcat cttgttttct ttccacaagt ccagcttttt ccatcctctt tatcataact
     4621 gtagttgtag atgcttttac tttcctttta tctgctaatt ctttttgact ttgcccatct
     4681 ttcatcttta aagaaaaaag tatttggggt tgaccatgat ataagcctaa tttatcaaaa
     4741 taaaaatgag ttcttataaa atatattcgc attaattgca agaaaacatc atttaatttt
     4801 tcttcagaat tgctattcat acttgccctc cttatttttt atattactta gtcggctaag
     4861 cattatttta ctatactgca tgcatttttt caatatattt ataaaaaatt catataaatt
     4921 tcatttatta aatattttta tatcatatag ttataatttt aataaataat agtaagattt
     4981 ttaattagtc agctacttat attttttatt gtaaagctta aaataaacct tttcatatat
     5041 tttcaaataa aaaagttgtc ccaaaataaa ttttgaaaca acatcatatt caacagtatc
     5101 ttgattatat aacattatga tactctactt atcttcatat ccatttacaa caacgtctaa
     5161 cttcccagtt tcaggtgata taattagacc atgtacaata ataccatctg gaattaaagg
     5221 atgccctttt attaaatcaa tactatcttt tacagattct tctgcagaat cgaatccatg
     5281 taaccattgt tttgtatcaa ttccagcact acgaagtgta agtattacat cattagatat
     5341 tcctctatct aaaattttac caagtaactt atccgtatca acattacaca taccacatcc
     5401 atgatgccct acaaccataa cctcatctac atcaaattcg taaattgcaa ctaaaacact
     5461 tctcataata cttccaaaag gatgcattat agtagctcct gcatttttta ttagtttcac
     5521 atctccattt ttcaaattca tagccttagg caaaagctca gttaatcttg tatccataca
     5581 agataagact actatttttt tatttggatg ttttgatgtc acatattgtt catattcttt
     5641 gttctctaca aatgatttat tatattctaa tatctcttca agttttctca tatgtaatat
     5701 caccctcttt aaaaattttt ttatta
//
LOCUS       DAELTA010000202         5704 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.203, whole genome shotgun sequence.
ACCESSION   DAELTA010000202 DAELTA010000000
VERSION     DAELTA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.203"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            232..414
                     /locus_tag="KPG13_003310"
     CDS             232..414
                     /locus_tag="KPG13_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0986999.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1241..2155
                     /locus_tag="KPG13_003311"
     CDS             1241..2155
                     /locus_tag="KPG13_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG0987000.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2545..3192
                     /locus_tag="KPG13_003312"
     CDS             2545..3192
                     /locus_tag="KPG13_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG0987001.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3359..3928)
                     /locus_tag="KPG13_003313"
     CDS             complement(3359..3928)
                     /locus_tag="KPG13_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987002.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3933..4832)
                     /locus_tag="KPG13_003314"
     CDS             complement(3933..4832)
                     /locus_tag="KPG13_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG0987003.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4843..4992)
                     /locus_tag="KPG13_003315"
     CDS             complement(4843..4992)
                     /locus_tag="KPG13_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987004.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4985..5569)
                     /locus_tag="KPG13_003316"
     CDS             complement(4985..5569)
                     /locus_tag="KPG13_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG0987005.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2089 a          857 c          661 g         2097 t
ORIGIN      
        1 taatttatat taaatatcta ctaactcaaa tattcataat taaactttaa actggataaa
       61 ttttctttta agaatgaaag tttatccagt tttgcgatta taataaccag ttttacatat
      121 ccacttgtaa aatatatcta agcaatatat catactatat agatttatga gtgaaataat
      181 atctgctatt tactgaatat ttttattttt cgttttttag ggggattagt tgtgtttaaa
      241 gataagattg atgaatgtgt tcatataatg actgcatata ttgctagttt aaaagaatac
      301 tattcattca tagaaactca aattggtgat tttattaaaa aatatggaga agatgttgta
      361 gagttatgtc tccatagagt gatgattcta ctatgcgaat gtggtttggc ataaaattaa
      421 atttattaaa atgggtaaga agaatcctat ataatattct tcttacccat tttaatatat
      481 caatttcata gcttatcgtt taaaatattt tacatccatt actagacaac tttacattct
      541 ctttttaatt ctagtatatt taaatcattt tagttgctcc ttatttattc ttattacttg
      601 ttttatttat aaaataattc tttttaataa tgttttttgt tgatattcct atcacacatc
      661 caattattta tcattgtctt gaaattgttt gcatacttaa tttaatccaa ttgaaacacc
      721 ccagcataga atcccctgta aaatagaagt tggattaaat cctaacatct atacagaaaa
      781 tactactcca agcacaagca atataatgga atatatttat cacttaattt tttatacttt
      841 ttaaatccca tacctaaaac attaagtgca gctattagaa ttaataattg ttgtggtata
      901 aaattatgct tgcttcatat ttattcattt tttgctataa aagttcttgt tttacaaaaa
      961 acatttgttt tgtatcaaaa taatacttat aaattacata attctcataa aaatttctag
     1021 tattacttgt actcttaata aataatagcc aacaagtacc ttaaatcatt atatctatat
     1081 ctaataagaa acacagtaag ataatctttt cgcccataac ttaataaaaa ctgttgatat
     1141 tagtagcaat tgaatttagt taatttattt ataatataca taaatttaaa tattacgaat
     1201 aaagtattat aaataggata ctttctaagg ggaggaaatt atgatagata atcaaaaata
     1261 tgttatttta tcactagaat tacatttatt tttttcaaga attatgaaag agcatgctct
     1321 ttttttagaa gcaggattca caaataaaaa ttataatctt gctatggaag ctgaccacta
     1381 taaaaagcaa tttgaagatt tattatcata cactgttagt gctagtaatg gtataattag
     1441 acctgatata ttatattcag aagagcttgt aactactctc acatcagttg cagaacaaaa
     1501 aacagaagag tttacaggga tagaaataaa caaaaacatc actacaagag aattaaatct
     1561 acaaagtggt gtaaacccac aagttggtca agacttagtg aactatgtag ctcaacttaa
     1621 ctctgatgca ataagattac ttgatgggct tattaatttt aaagaaagag tcttagatgg
     1681 tgtactatca tgtactatat ttacatcaaa ctaccctcta cttcttgaac atataataca
     1741 tgaagcaaat ttatatcgtt cttatgtagt tgaccttgaa aataaaatag atattgagtc
     1801 aaaaaacgct aaagaaatag aattattctg ggaccatatt atgatggaac atgctctgtt
     1861 tatgagagga ttactagacc cctcagaagg tgaactaata aatacttcaa atgattttgc
     1921 tataaaattt aatgaattaa ttgaaaaaac aaacgaaatg actgattcta atatcaagaa
     1981 cattacagaa gaaactctaa atgaaactgt tgagtttaaa gattttaaag aagcaggagc
     2041 atcaggaata gaacagtgta agataaaatc tataatatta ccacttttag cagaccatgt
     2101 tttaagagag gcaaatcatt atattagaat attggagagt tataaaaaca tgtaattaaa
     2161 aattactttt aaaataatct ttgcatattt agataaaata aaggtacagt taactgtacc
     2221 tttatttttt ataaacttat aaaatttaaa caagttattc ataaaataaa ataataatca
     2281 tctataaccc acaactttat tttatattaa ataaaaagta ttagtggtta tgttttataa
     2341 gtatttcttt attcagttat ttattaaaaa ctaatagtaa atcttatact aatcgcatat
     2401 ttatgttata actatattgt aactattttc gtaatcttag gaaaagatta cttataaaat
     2461 taaccttata caataatata tcgtataatc ttaattagca taatagttac aatattaaaa
     2521 tttaaatatg ggagtgattt tagtatgata agacaaacac cattagagaa acgatatgac
     2581 aagctaggtc ctcatattgt aacagcactc aaaaaacgtt attttgatgc ttattactgt
     2641 aaaactagaa atgaagcttt gcaacaaatt ttagatttaa ttcctcaaaa tcatgtagta
     2701 tcatggggag gatctgaaac acttaaagaa atgggcgtac aaactgctgt tagagagaaa
     2761 ggatttaaag ttattgatag agatttagca aaaactccag aagaaagaac tgaaattatg
     2821 cgtcaagcac ttctatgcga tacttttttg atgagtagca atgcaattag tgaagatgga
     2881 caactcttta atattgatgg aaatggcaat cgtgtagctg caatgatatt tggtccaaaa
     2941 aatgttatag ttgtagctgg tatgaataaa attgtaaaaa acattgatga tgcaatacaa
     3001 agaactagaa caattgcatc tccagcagtt gttcagcgtt ttcaagatgt caatacacca
     3061 tgctatatta atggaagttg tatagattgc acaagtcctg aaagcatttg tgcatacatg
     3121 gttactacaa gaatcagtcg tccagcaaaa aaaataaagg taatattggt aggagaaaat
     3181 cttggtctat aaaatataca tacatgtaaa tagctctata aataataaaa tcatatgaaa
     3241 taacttattt ctaaaagtgg tagaataaaa gcttctacaa gactttcatt ctgccacttt
     3301 tatattcttt taatttgtgt ttctatatta ttaattgctc caattttcat atatattact
     3361 aactattttt attttcataa tttttttgtt cttcatcaag ctgtttataa taatctttat
     3421 tatagtcagc tagataatct ataccacctc ctgtaatcac atttaataat ttcattgcag
     3481 attcttgatt aactttacaa acataattat caccagattt aacaagctct aatgtaatat
     3541 taaattcttt tttctttgca gaatcaatat ctttcatagt tggatttggc atcatttctc
     3601 ctagtttagg aggattcttt tttaaaatag aaaccatatc agtattgtta atttttaaat
     3661 ctacagtcac tttatcctta ttatttttta tatctgaaat ttcccaagtc atatttttag
     3721 aatacatttt aaatccttct ttatcttcgt atataagatt acctaaccct gattttggga
     3781 aatacttatc aacagtatct gcatcttgtg tttttattga attaaacata ctatccactt
     3841 cattttttat cttattatca ttagtacaac cagctaacac cagtactgat attgcaaaca
     3901 ttataataca tagaataaat ttcttcatta aattatatcc ccctatactt ttttgcacaa
     3961 atattattct cttttttatc ttttgaaaaa gttgtagtaa ttgcattttg tggacaaact
     4021 tttatacatt caccacacct aatacagtct ggactattta catctttata tattggtata
     4081 tcaagcttac attttgtttg acatgctgta caatttgtac atttatcttt atcaatttta
     4141 aatctatata ttgaaatagg attaaaaaaa ccatagattg ctcctaaagg gcatatatac
     4201 ttgcaaaatg gcctataagt aatgattgac atcaataata tcaataacaa aatacttaac
     4261 ttccagctaa ataaaaatcc tattgattct cttaaattct catttcctaa aataagtggt
     4321 attcctccca aaagtgttcc tgaagggcaa atccatttac aaaaccaagg atttccaccc
     4381 cctgctacat ttacaatagc aactggcaaa ataattacaa atattattaa gactatatac
     4441 tttagatatt ttagatattt atcaccataa acttttttta tctttactgg aaagggtact
     4501 ttatgtagta aatcttgtac aagtccaaag ggacaaagcc aaccacaaac aaatctgcca
     4561 aaaaaagcac caaatgccat taaaaaacca acaacataaa atgagaattt atactctctg
     4621 cttgataaaa ctgcttgtaa tgaacctatt ggacatgctc caagtgcacc tggacatgaa
     4681 taacaattca atccaggtac acataatttt ttactttctc ctccatatat agttccttct
     4741 aaaaatccat ttacatatcc atttgttaat gctgtaaatg caacttgagt aataagtctt
     4801 atattattat ttactttaga taataacttc attcctcttc tcctaaccaa taccaataca
     4861 ctcaagacaa atcataattg ctttagttaa tactgtattt gtttctccac gatatgtacc
     4921 taatgcaata aatataatac taattgcaat aatagaaatg ccttttttat tatctttaat
     4981 aaagttactc attggctttt accattttta gtctttcatc tataattttt ttccaatctt
     5041 ccttactatt tgcacctaca accttctcac ccacaaagtt tcctttcttg tctacaaaat
     5101 aagttgttgg aaactccata actcttttaa aatcagtttt tttaagttct tcagatacag
     5161 tcaattgttg gtaagttgca tctgactttt tcataatgtc tttaactaaa tttttctctt
     5221 catttgaaag accagttctc atatcagttt tatcaacttc tacaactaat ccctttatag
     5281 ctacattgga attattactc tcatattctt ttgataatgc ttcaagctct ggcatttcac
     5341 ccacacatgg tccacaccaa gtagtccaga cattaataag agtaagttct ttatttgaaa
     5401 atatctcttt tgttacctta tttccatcaa catcctccgt atctaaatta ctaaaccatt
     5461 cactattatt cttgtttttt tctactggct tagattcttt tttgctacat ccagaaatca
     5521 tacctaaaac acaaatacat cctaaaacta ctatgaaaaa ccttttcata tctcacactc
     5581 ctagaattta tatagtatat aaaatcgctg cttgaaataa aaataactta tcttctatat
     5641 taaagtaatt atatttaata aataagagct aatttaaaaa aagatataca gttaaaatct
     5701 attt
//
LOCUS       DAELTA010000203         5513 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.204, whole genome shotgun sequence.
ACCESSION   DAELTA010000203 DAELTA010000000
VERSION     DAELTA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.204"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            30..815
                     /locus_tag="KPG13_003317"
     CDS             30..815
                     /locus_tag="KPG13_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG0987006.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1094..3004
                     /gene="phnW"
                     /locus_tag="KPG13_003318"
     CDS             1094..3004
                     /gene="phnW"
                     /locus_tag="KPG13_003318"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG0987007.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3363..3911
                     /locus_tag="KPG13_003319"
                     /pseudo
     CDS             3363..3911
                     /locus_tag="KPG13_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4013..4222
                     /locus_tag="KPG13_003320"
                     /pseudo
     CDS             4013..4222
                     /locus_tag="KPG13_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            4791..5198
                     /gene="tnpA"
                     /locus_tag="KPG13_003321"
     CDS             4791..5198
                     /gene="tnpA"
                     /locus_tag="KPG13_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG0987008.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            5200..>5513
                     /locus_tag="KPG13_003322"
     CDS             5200..>5513
                     /locus_tag="KPG13_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0987009.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KS"
BASE COUNT         2292 a          549 c         1018 g         1654 t
ORIGIN      
        1 aaaatatatt atatttagga ggaaaatgaa tgtctttaaa ttcgatagat agaaaaaaag
       61 aaatacttaa attattagat tcaaaaggga aggtaaacac taaagaatta gtaaaaagat
      121 tagaagtatc atcagaaaca ataagaagat atttggaaga attagaaaat gaaaataaat
      181 taaaaaaggt atatggtggt gctataaaaa taaattatgg agtgattgaa ccagaacata
      241 taaagagaaa caatactaac ctagataaaa agcaaagtat tgctaaatat gcagctaagt
      301 ttgtaaatga caatgagtca attatcatag atgaaggtac taccaatttg cagattgtag
      361 attatataat atctgtaaac aatttaaaaa ttattacaaa ctcttatcca gttctatcaa
      421 aattgatttc ttatgagaat caaggtttgt ttgatggaga agttatattt attggtggaa
      481 aagtaaattt aaaacaccaa aggacagcat gtccaatgtc aatagatact atgaaaaact
      541 tatacgtaga taaagctttt atatctagtg aaggtatact tgatagtttt ggaattagta
      601 gtatagacca aaataaggca ttgttgtcaa aagaatatat aaaaaattca aaggaaagtt
      661 ttgtattatg tgatagttct aaaataggaa ttgctagtat gtataagata gcagatttag
      721 aggaattaga ttttgttatt tgtgatgtag aacctccgaa tgaatggaag gataagttaa
      781 atgagataaa tataacatgg atagaagcaa aataaaccct tagttttata taatacaact
      841 atgctcaata caactaaatt cataaaatat agtcagttca tagattaaga ttagaaagta
      901 aattttagat tgtaacttag gattttatgg gaatatgata caaaataaaa acaactaaat
      961 acaactaaaa acagaaattt aatatatatt taacctaaat atgatgtata attaacatat
     1021 gaaagttata ctttaaaagt aaaaacaatt aaaaacacaa aaattctaaa aagaatctat
     1081 ggaggctata aatatgaaaa agatatatgg agaaaaaata aaagcagtag tattcgattg
     1141 ggctggaaca acagtagatt atggatgttt tgcacctctt aatgtattta tagagatatt
     1201 taaaagaaga ggtatagatg taacaatgga agaagcaaga aaacctatgg gaaaattaaa
     1261 aatagaccat ataagagaaa tgtgtgaaat ggatagaata aagaatattt ggtcagataa
     1321 gtttggtaaa gtgccaactg aagatgatgt aaatgaactt tatgctgaat ttgagccaat
     1381 gttatttgag acattagaag agtatacgac tccaattcct catgttgttg aaactattga
     1441 aaagttgaga aaaaatggat taaaaatagg ttctacaacg ggttatacaa gagagatgat
     1501 gaacatagtt gaaccaaatg cagcaaagaa aggatactca cccgattttc ttgtaacacc
     1561 atcagaagta tctcaaggaa gaccgtatcc ttggatgtgc tataaaaatg ctgaagcttt
     1621 aggggtgtct cctatgagtt ctatggtaaa agtaggagat acaataagtg atgtaaaaga
     1681 aggcgtaaat gcaggaatgt ggagtgttgc tgtaataaaa ggttcaagtg agttagggct
     1741 tacacaagaa gaagtagaga acatggataa ggaagaatta aaagctaaga tgagtatagt
     1801 ttcaaagaaa tttaaagagg caggtgcaca ttttgtaata gaaactatgg ctgagttaga
     1861 agatatacta ataaaaatag agaatgaaac tattaaatca gattttgtac cagaaaatga
     1921 ttacatatta cttacaccag gaccactatc tactactaaa tcagtaagag catctatgtt
     1981 aaaagattgg tgtacttggg atgttgaata taataaccta gtacaagatg ttagaagaag
     2041 attagtatct ctagcaaccc aaaatacaga taaatacaca agtgtattaa tgcaaggaag
     2101 tgggactttt tcagtagaag ctataatagg aagtactatt tcaaaagatg gtaaacttct
     2161 tgtaattgca aatggagcat atggaaagag aatgaaagat atatgtaatt atcttaacat
     2221 acaatttgtt gactgcacat ttaaagatat agaagcagtg gatttaaatg tagtagaaaa
     2281 tttattaaaa gaaaacaagg atataacaca tatatcaatg gttcactgtg aaactactac
     2341 aggtagatta aatcctatac aagaggtagg gaaacttgca aaagaatata acaaaattta
     2401 tatagttgat gctatgagta gttttggggg aattgagatt gatgttgaag attttaatat
     2461 agatttttta gttagtagtt ctaacaaatg tatacaagga gtacctggat ttggttttat
     2521 aatagctaat aaagagaaat tatctaaatg taaaggtata gctaaatcat tatctcttga
     2581 tgtgtatgcg caatgggaga ctatggagaa aaataatggt aaatggagat ttacttcacc
     2641 tacacatgtt gtaagagctt tttatcaagc tttattagaa ttggaagaag aaggttctgt
     2701 tgaaaaaaga tatgctagat ataaagaaaa tcagtttaca atagcttcaa gattaaaatc
     2761 tttaggcttt gatactttgg tgaatgataa tgctcaatca cctgtaataa caactttctt
     2821 atatcctaaa aatgcaaagt ttgaatttat ggaattttat acttatttaa aagataatgg
     2881 atttgttatc tatccaggta aattgactga tatagataca tttagaatag gtagcatagg
     2941 tgaagtctat ccaactgata tggagagact agctgatgta atagaaaaat ttatcaatag
     3001 atagaagaag atagtgtctt gtaggttaca gaatagtgcg caaagcctac tcctttaggt
     3061 gtgtgagatg atagcacaaa cagtgtataa aaagtattga cagtagaatt taaagactta
     3121 tattttagaa gtttatacaa tgaatatata aacattgagt aatagggata tattttattt
     3181 aaaccacaat ctaagaaatt agatttatag tttaaataaa gcatttccct attattttta
     3241 tgcctattta tagaaaataa atgatagttg ctttattaag tattattatc atacttgaca
     3301 acgtaacaat gttttgttat attaggttta aagataaaat acggtaaaga aaggatatca
     3361 ttatgaatga agaattaagt tcactaagat ttgatggtgt tggaaaatgt aaaggtggaa
     3421 tttttgataa tgttaatata tcaggaacag gcaaaataga aggaagtgta aaatgtagta
     3481 aatttgattc ttctggttca gcaaaagttt ttgggacaac agaatgtaat gaatttttta
     3541 catctggttt aagtaaagta gaaggaaaca tactggcatc aaaagttgag atatcgggac
     3601 ttcttagatg tacaggaaat attaattctg ttgaaattga tactagtgga attattaatg
     3661 tagaaggaag tataaaatcg gatattattc atggagaagg atatttaaaa gcaaatgaga
     3721 atatagattg tgaaaatatc aatttaagtg gaatcttgga gtgtaatgga tttttgaatt
     3781 gtgaagaagt taatattagt ttaaatggaa cttctaattt taatgaagtt ggagcaagta
     3841 gtataaatat aaaaaagggg catggagatt attgcaaaaa atttcgtatg ctagaaaaaa
     3901 agcatatctc tagtagataa ttatgatgtt ggaaatattg aatacagtga taatttatca
     3961 gtttcagata attctactgt aaaaaacagt ataaaggtgg gctagaagag ctatggaaga
     4021 attaatatct aagaaagatt tattgagcaa aactaatata tcttatggac aattatatag
     4081 atggaaaaga aaaaacataa taccagagga atggtttata aaaaaatcag cttttacagg
     4141 tcaagagact ttctttccaa gagataaaat actagaaaga atagagttaa tattgtctat
     4201 gaaagaagag acttctcttg attaaaaaat agctatgcac acttgtgact ttagtcatga
     4261 gttagtagct aaaatagtca gcatataggg aaacttatat gtagagatag gatagaacct
     4321 atccaatact acttgaattg ctggaaaccc ctaaagctaa ccaaactaaa acataaggat
     4381 gaaataaacc taagtgtgaa agttacgaaa gtagaaaaaa ttggttagat ggtataaggt
     4441 taaatcctaa gtactttaaa atgggcaatc agcaggtaag ctccgaatag gaaaaacttc
     4501 aacgactatt cctcttgagg gaagtacact acaagctatc ggtagtggaa gtgggtagac
     4561 cttaacggat aatgccgaag gataagatat agtctgtgct tgcatgaaag tgtaagaagt
     4621 tcataagaga actgcatagg tagtagcgaa cttatgtgaa cgacaacctc aaaaatgata
     4681 aaagttaaga tttgcgtttc ttacgtatgt tatttaaaaa taaatataaa aaccttaaga
     4741 atacttgttt taagtaatta ctatgttaat attatgttgg gtgatgaaaa atggaagtga
     4801 ctcatggtag agggtatgtg tatttagtac aataccatat agtatggtgt gtaaaatata
     4861 gacatccagt attaacaaat gaaattgaag aagatttaat taatatatta aacaaaatag
     4921 ctgaagataa taaatttcaa atattagaat gtaatagtga taaagatcac atacatttac
     4981 taataaattg ttcaccacaa cactatatac cagatataat aaaagcatta aaaggtgtat
     5041 cagcaaggct gttaatgaaa aaacatggag aacagttgaa aaagaaatta tggggaggac
     5101 atctttggaa tcctagttat ttcgttgcta ctgtatcaga aaatacagaa gaacagataa
     5161 gaaaatatat taataatcag aaaaaacaag taaagtgagg tggcagtagt ggaaaaagct
     5221 tataagttta gaatgtatcc aaataaaaaa caacaagagt taattaacaa aacttttgga
     5281 tgttgtagat ttgtatataa taagtatctt gctaaaagaa tagaagttta taaaaacaat
     5341 aaagaaactt ttacttataa acagtgtagt tctgatttaa ccaatttaaa aaaagaatta
     5401 aaatggctta aggaacctga caaattttca ctacaaaacg ccttaaaaga tttagataat
     5461 gcatataaaa aattctttaa agaaaaagca ggatttccta aatttaaatc aaa
//
LOCUS       DAELTA010000204         5401 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.205, whole genome shotgun sequence.
ACCESSION   DAELTA010000204 DAELTA010000000
VERSION     DAELTA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.205"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(949..2028)
                     /locus_tag="KPG13_003323"
     CDS             complement(949..2028)
                     /locus_tag="KPG13_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0987010.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(2112..2768)
                     /locus_tag="KPG13_003324"
     CDS             complement(2112..2768)
                     /locus_tag="KPG13_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0987011.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            3090..3296
                     /locus_tag="KPG13_003325"
     CDS             3090..3296
                     /locus_tag="KPG13_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987012.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(3449..3877)
                     /locus_tag="KPG13_003326"
     CDS             complement(3449..3877)
                     /locus_tag="KPG13_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG0987013.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(4353..5291)
                     /locus_tag="KPG13_003327"
     CDS             complement(4353..5291)
                     /locus_tag="KPG13_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG0987014.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         1662 a         1063 c          676 g         2000 t
ORIGIN      
        1 tatatagaaa tacatctaag aaagaatatt attaaattgt tccaattagt attatttttc
       61 ttatagaaat aaaaaaactg ttggagtctt ttagaaaaat tattttttac ataccttttt
      121 tgcttagata gtctatcttc acagctattt tgtatataca ctcttctagt tcatgatttt
      181 attttaatat cttgcttata ttgctgaaat aattgaattc ttttatctat catattgtct
      241 cttatatcac aaacatattg tttcttatat cacaaaaatg aaagcattct tatatcttca
      301 atatataaac ctgctttttt taatcttgta ataaatatag gatactctat attctcttcc
      361 tagaaataac taataccact tatttttcta ttatatagaa agtaataaat ctttttctta
      421 ataaaattca atagtaagtg gaataaaatt ttatatgaat aagaacatta taaaaatact
      481 ttatacaata gtcaataaaa gatattagct tcaaattatg ttaaagttgt attatttaaa
      541 gtgaaaaaac aaataaaaaa gaaataatag tataagtaga aaaatatact taaaacaagg
      601 cgaagcctct cttgtatcta aatcaagtag agaattaaat ctaaaaaaga aaataaatat
      661 aaaagatgac tcctgtacat tacaagaatc atcttatggt ttcataatca ctccatggtt
      721 tttaactgtc tgatataaag gtctcagttc actcatagca tgaccacctt ttccgattgc
      781 tgaccccgtc acttatggac aaaatgtcca taagtgatag tcctctctta aaaccctcta
      841 aatagatata tctcaagcac cgcattgaaa ccagttgctg gaaacaaact gaagcgttct
      901 ggaatagtaa aagaaattgc caccatatcc acgttactgt cctagttgtt actttgctaa
      961 cttcggagcg atttcctcta acaatacgca gtcaaattct gcctttttgc taagaaatgg
     1021 cagatggtct gcatctggta taaaaaaaat ctgtttgtct ggcgccacta tttcatcaaa
     1081 aaatttcttc gctggaagat aggggctatt atagtcattt tctcccatga tgtagtatac
     1141 agggacctga tagtcaactc cataatttct tatgtcagct tcttccacta aaaaccgcaa
     1201 tacatcacct tgattgtcaa tcgcatttgg attgaaaatg gccgtatttt ttaactctct
     1261 caatgaataa tacggggttg ccagtatact gattatattg cccaaatcca tatttataga
     1321 aagcccatac ttgctttggt atgttcttac tgtcataaga gctgccaaat agcttggacc
     1381 atattttgct ggcggatatg gtgcaatagc ttccagcgcc ttgatatctt tttgcttccc
     1441 tgcagctttc accatttcaa gtgtcttttc atagcccacg cgctcattgt ccaccatatt
     1501 aacggtctgc gcaatcccaa tgtacgcgct gtaatcctcc ggataggttt gcacagccat
     1561 agttcccaga actgttcccc agctatgacc tataaggata attttctcct tgttgaactc
     1621 ttgcttcaaa tatgctgtca cctcatggac gtcttgcaga acacgctccg cgctcatggt
     1681 ctgcaaaact gctgccggat cgttcgcaaa gtatgttttg cctacgtttc gctgatccca
     1741 gttcaccaca gtaaaatcat tttcccactc atactggtac agatgtaaaa atggcatcat
     1801 tgaattacca ggacccccat gaacccaaag gatgatgggg ttgtctgcgt tttgtccccg
     1861 gatatataac gcctgctgta tgccgccgat ctccaccatt tccatgcggt cgattccagg
     1921 agcttctatt ttatggcttg tacgtataat cgtgggggct atcagacgca aaccggcaaa
     1981 cgccagaaca aaaacagcaa ctagtatgca aagtgtgatt aatatcattt taagtacctt
     2041 ctttcatgaa tttttttagc catagatatc tcctctaaca ttaaaaaaaa tatgttgcta
     2101 ttttagtttt gttactctac ctgtcccatt ggtttccttt cataaaaaat attaattgat
     2161 tctataatct catcttccag gatatcaact cctatttgtc tcataagaaa ctgtaagaat
     2221 tgaaaccgtt tacgaagtgt aggaaagtcc ccctgaaata catctgtatc acaccaaaag
     2281 ttaaagagca gcaaaattac ctctgcgcat tcatctggga actctgtgat gatgcttcta
     2341 tcctctatgc cttctcgtaa aacatttgct aatattggtg ctagtttttt caaattatcc
     2401 tgtgtaaatg ccagtatgat atgcggactt tctatcgcgc ttgtaatcat gttactagat
     2461 tcttttatca cctcatccgt taaatgccgc ctaactaaac ttctaagctt ctcttttgct
     2521 gtaagtccat gcatttcacc gagccattga tttactgtct ctattatctg ttcaaagcgc
     2581 ctttccatga ccacattaaa aatatcttct tttgaactga agtgatgaaa aattccgccc
     2641 tttgacattc ctgaagcatc aacaatattt tgcatacttg ttttgtcata tcctttttca
     2701 gcaaacaact ttgcagaaat ggtaatgata ttttcaatag cttgctttgg attgttcttt
     2761 ctagtcatta tataactcct tcttattaaa aaccgactgt tggtttttat tatataagct
     2821 attcctgctt ctgtcaagta acattgtgtt tatggcaaaa caataaggtt tggtggttag
     2881 acaggtatta attagactaa tgcgactttc ccctagatgg aacccttggt aaatttgcaa
     2941 tctttaccgg ctttgattat atctgtaagc aaggcaggta tggccacacc tgccctgttt
     3001 gcccctcgga tattcattcc tatccctcct ctccttagcc attacaccaa aacgccgctc
     3061 attgtctcca atggcggcgt tttaaagata tgatttctca tgctgctgaa atatgcaaaa
     3121 aatataagat gccctttatt atttttccta caatagaaaa gaaaagtatt gaagaatggg
     3181 ttaatattgg agcaaatatg ctttgtttgg cacaagatac tacctttatc acttatgtta
     3241 tcaatcaatt attatctttt tataatgaga ttgtaccaat tcaaaaacaa cattaaatta
     3301 taaatctaat tttatattaa aagagactaa tattttatct atctctatta aggatatttt
     3361 cttatgtatc ataatattag tctctcttat tattatatac tgctatcatc attagttgct
     3421 atttatacta tttttataat atacaaattt attcagctaa cgttttgcct aattctatac
     3481 actcatcttt tgcttcttca tttgggtctt ccattgctgt aattccttct ccaccaataa
     3541 ttgtagcacc agcattttgc atacgttctt cccagtctct catccattca caatttcccc
     3601 atccatatga accaaataat ccaatctgtt tacctgatac catagattct aattctgaaa
     3661 caaatggctc catatacccc tcttctagtt gctctgctcc catagatggg caacctaatg
     3721 caaatttatc ttcttctttt aaatcacttg gtttcagtaa gctcacatct aaaacttctg
     3781 gtgtcttacc ttttaacttc acaccttcag ccacaaaatt tgccattttt tcagtatttc
     3841 ctgttccact ccaataaaca atatatattt tactcatttt tacctcctgt agtatttttt
     3901 aattaatcaa tagttagtta tagctaacta acatctaaaa aacaaaagat ttctttatct
     3961 gaaatctctt tatttttcta aatatagaat atcatggttg attttgaaat tcaatactta
     4021 ttgataatat ttatcattta tattttatat tcattttata atatcaaaac actgtatcgt
     4081 atttctaatc tttcatagaa aaatttctaa cttatagtac ttttgcatct cttataaatc
     4141 ttttatatgt ataagtacat agaaaaacac accttttcca gatgtgctat ctataatatc
     4201 gtatatttat ttttcaattc taactagcta atatgtttat aaattttatc acttaatacc
     4261 tttatacatt gatgtaaaat cttacaccta agtaattttt attatttgtc tcgactctat
     4321 ataatatagc tattttatca tcaaatcaaa aattatcttt tgctattatt cttgtattta
     4381 taaagattta aatataagta tccatttcct tgttccgttc taggtattcc aagcgctctc
     4441 gtacatttta ttaattgtgc atacagttca gcttcatcta aatctctttc aaactcctct
     4501 cttgcccatt ctttaattag tgctaatgag cctgatacgt atggtgcact catactggta
     4561 ccactcataa tagcaagttt attatccata taagtcgata taatatttct tcctggagct
     4621 accaaatcta ttgtagtatt tgaattacta aacttttcaa ccaaatagtt ctcatttatt
     4681 gcacctactt ctattacctc agcataactg gctggataac tatactcact tgtactagaa
     4741 tcaccatctc cattattacc tgctgcacac actacagaaa tattattttt tactgcttgc
     4801 atgacagcat tttttaaatt cttatcatct ttgtttcccc caagagacat agatataata
     4861 tcaaccttgt tatttacagc aaagttaata gcattaatta tactttgata tgttccagta
     4921 ccatctttat ttaatgcttt tgctattaat aatttacaat ctggagctac tcccataact
     4981 tcattattat aattagatgc agctataata cccgccacat gagttccatg accattaaaa
     5041 tcctcatata tatttttatt tccattactg tcatcactaa aatttgcacc accaattatt
     5101 tttcctttta aaagaggatg agatatatca caacctgtat ctattatacc aactacaata
     5161 tttttaccag tataaccttc atcccacatt cccctagcat tcatttgttt tattccatat
     5221 gggaatttat tttttgcacc tcttagattt tcatttattt catatggaat tagtttcatt
     5281 cttttattca tcatatatcc tcctaaaaat tttattgctt ataaaaatca tgaattttat
     5341 tttataatag gtttataaat caaattaaaa cctatatcta cttatttata tttatgatat
     5401 a
//
LOCUS       DAELTA010000205         5365 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.206, whole genome shotgun sequence.
ACCESSION   DAELTA010000205 DAELTA010000000
VERSION     DAELTA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.206"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            258..1721
                     /locus_tag="KPG13_003328"
     CDS             258..1721
                     /locus_tag="KPG13_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0987015.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1777..2913
                     /locus_tag="KPG13_003329"
     CDS             1777..2913
                     /locus_tag="KPG13_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG0987016.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3149..4264
                     /locus_tag="KPG13_003330"
     CDS             3149..4264
                     /locus_tag="KPG13_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG0987017.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4416..5285
                     /locus_tag="KPG13_003331"
     CDS             4416..5285
                     /locus_tag="KPG13_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG0987018.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2060 a          549 c         1002 g         1754 t
ORIGIN      
        1 ttcattatat gtattccaat ttatattgtt tgattattat tcaaacacac gtcgttaaaa
       61 attttataaa tttatgttta gatatgatta atactcttga ttttagaaat aaatatttgt
      121 agattgtaga ttaaagataa tataaataaa tagaaggtag attttttaga tatattaata
      181 taaagattat ataaaatatt gaaatatagc tgtttaaatt attgtctata aatataaaat
      241 gaatggtggt gagttaagtg accgatttag ttaacaccat taatggaatt gtttggtcac
      301 ccgtattagt tgcaatgtgt ttgtgtatag gattatattt ttcattgaaa ttaaaatttt
      361 ttcaaataag agatgtaaaa gaaatgttta ctctcttgct tgagggagat agttctcatg
      421 ttggtatatc atcatttcaa ggttttgcta catctatggc tggaagaata gggactggaa
      481 atatagctgg tgtagctgta gcaatagcaa tgggaggtcc aggagcatta gtatggatgt
      541 gtataatggc tacattaggt tcagcaactg catttgtaga atctacttta gctcaaatat
      601 ataaagatga gcatgatggg cagtatagag gaggacctcc atattatttt gaaaaaggtc
      661 ttgggtggaa aaagtgttca gttttatttt caatagttgc aataataagt tatacatttt
      721 ttatgcctgg tacacaatca aacactttat atctagcaat gaatcaagca tttggatttt
      781 caaaaatgac aatagcagta gcatcttcta ttgcattagc gataataatt tttggaggaa
      841 ttaaaaggat tggtttattt gctgaaaaag tagtacctat aatgggtgga gcatacctta
      901 ttatgacatt agtaataatt ataacaaata ttagtttagt tccagaggct attgtaacaa
      961 tgtttaaatc tgcatttgga tttggttcag tttttggagg aatgctaggt tcagcaatat
     1021 cttggggagt aaaaagaggt atatactcaa atgaagcagg agaaggttct gggacttatg
     1081 gtgcggcagc agcagaagta agtcatccag ccaaacaagg attagttcaa gcattttctg
     1141 tttatgtaga cacattatta atatgtatgg ctacaggtat tatgattgta ataacaggaa
     1201 tgtataatgt tacaggtgtt tgcacagcac taccaggtgt agaaccggga ccacaatatg
     1261 tacagagtgc aataaataca ttacatccac aattaggtga tatttttata gcaatagttt
     1321 tattattatt tacatttaca actttattag ccttctcata tatgatggag actaatgtat
     1381 cttacttagt aactagaatt aaagcagaaa aacatgaaaa gtacataatt aatgtctgta
     1441 gatttatgct tatagcatgt gcagcaatgt gttgttttat agaaccactt acaagctggg
     1501 cattaggtga tatatgtata ggtgtattaa catatataaa tttaattgcc atctttacac
     1561 taaaaaaacc agctataaag gcatattatg attatataag acagaagaag atgggaataa
     1621 aaaggagtga tagaactttt aaccctatag agttagatat aagaaatgct gagttttggg
     1681 aaaatagata cttaaaagag gataagcatg agttaaatta aagttataat aaaatataaa
     1741 aattactaaa taaatttagt gggaggtaag attggtatga aagaaagaat aatacaagtg
     1801 gctgattcaa agaaagaaaa aattctaggg ctatgtcaat ctttatatga tgaaccagaa
     1861 attgctttgc aagaatataa atctgctaaa aaaatatcag aatttctaag agaagagggt
     1921 tttgatgttg aggaaaactt ggcaggtatg gctacagctt ttaaagcaac caagaaaaat
     1981 ggagatggtc ctaaaatagc atttatagca gaatatgatg ctcttccagg caatggacat
     2041 gcatgtggac atcatttaat tgcatctatg ggagttggag ctggaatagc tctttcaagt
     2101 atactggata cttacaaggg agaagtaagt ataattggaa cacctgctga agaaactgga
     2161 gatggaaagc cttacttgat agaacatggt gtatttgatg gctatgatgc agcaatgatg
     2221 atacatccaa attcaaaaac ttgtgtaaca cctgaaatta tagcaatagg aggtctagat
     2281 ttcatattta ctggaaaagc atctcatgca ggagctaagc catataacgg aataaatgct
     2341 ttagatgcag tagtactact atataataat ataaatgcac taagacaaca attagtagat
     2401 ggaacgagaa tacatggtat tatattagaa gcaggaactg ctgcaaatgt aatacctgat
     2461 atgggaaaag taagattaga gataagggca aaagaacaaa attactttga tgaagtagta
     2521 gaaaaagtga aaaattgtgc tagaggtgct gcaatagcca ctggatgtga acttgaattt
     2581 tatcactttg aaccaacttg ccaaggatta aatgaaaata aagttttggt agacatattt
     2641 acaaagatta tggaggaatt tggaatatat gaagatgaac aagtaatgtt aggttcaaca
     2701 gatatgggaa atttaagtca aataatgcca tgtatacatc ctttgatgaa gttttctgaa
     2761 aatggagaag aactacatac aaaagaattt ttggaggctt ctataaattc atatgcaaaa
     2821 gatagagtaa ttgatggtat aaaaatatta gctttaacag gatttaattt atttgaaaat
     2881 ccagaattat tgaaaaaaat gaaggaagag tagaacttag actattttta attatactct
     2941 ttaaaataag aagagtgtct caaaatgaac tttttagttt atgagacact cttttttgta
     3001 tgaaattaaa tatattttta ttattttaaa catgaaaaag aggcttatct gtttttatat
     3061 gacctttttt aacttgtagc acaaaatatc atgaatgatt acaattcgaa tgataaaatc
     3121 gattataaga tatataggag gggtacatat ggaaacttta gaagtaagaa atattgaatt
     3181 gagtggtaca aattatgaga taggatacag actaggagaa ttggttgcta atatgccaga
     3241 gataatagaa ggtcagatta ataaatctaa tctggtaagc aaaaaagaag aaaaagagat
     3301 gatagagttg tttgataaat attgtccagg tcttaatgaa gaattgcaag gttttgctga
     3361 tgcaattcaa gtgaattgta atcaaatttt atattatact atgacttatc ttaaaccagg
     3421 ttgtagtcaa gttgcattag caccagaatt aactgaaaat gggcatgtac tatttgcacg
     3481 taatttcgat ttctcacaca atatggagga ttttgtttta tgtaaaacta aggtaaatgg
     3541 aaaatatgca catataggta ctaccattat gcaatttgga agaggtgaag gtatgaatga
     3601 atgtggctta ggtgtaagtc agtcatcatg tggtattcct gtaggcaatt cagatggttt
     3661 aagaaaacca gcaattgtag gtttacagtt ttgggctgtt attagatatt tgttagaaaa
     3721 ttgcaaagat gtagatgaag cattagaata tttaaaagat atgccaattg catacaatat
     3781 taatttattg cttgcagata agtctggaaa cattgcactt gtggagactt tagatgggaa
     3841 aaaagaagta aatataatca acagtttaga aagtaaaaga gaatattttt tacactctac
     3901 taatcataca catattgata aacttcataa attagatcca caatcaatga aaaattctat
     3961 tcatagatat aaattaatta aagaatatat aaataagtct aaaaaaattg gagagaaaga
     4021 gttaatgaat ttattgtctt caaaatatcc aaatggctta tcttgtaatt actataatga
     4081 tttttttgga actttaaaaa gtatagttat ggatttaaat ataggaaagt ttaatatttt
     4141 atggggtgga ctagagaata aatgggagag ctactattta aaaaatgaca ttaaatctat
     4201 aacacagagg ataaacataa atattgaaaa agcaccatct gacttttttg atttcattga
     4261 ataaaaaatg tatttaatta ttctaaataa ttaggacata taaatcaact aaaagttttt
     4321 atttatcgta ttgaattagt tataatattt tagtatagta aaaaatagtg ctattttaca
     4381 tatttaaaga gtttaagtag aagagaggtg ccatattgga ttacttaaag tattttgaaa
     4441 aagctgtaat gtatattgag aataatttac atgaaaatat aactgtaaat gatgtggcaa
     4501 aagaaactgg atattcttat tatcatttaa cacgcttatt taagtctatg tttggtgaaa
     4561 gtgtaggaag ttatataaaa aaaagacgat tagtaagtag tacaaaagaa cttttatata
     4621 gtgataaaaa agtgatagat atagctattg gtagtggatt tgaatcttca gaagcattta
     4681 gcagagcatt taaatctata tacaaggtta gccctataga atatagaaaa aatagaattg
     4741 atgtatttgt tggtaaaaag aaaaaattag agttagattt tatgagacat cttgttggaa
     4801 atataacgat taaaccagtt ataaaagaaa tagaaaaaat aaaggtaata ggtataaagg
     4861 ataaagtaat tttagaaaat aattcattac ctgacttgtg ggagaaattc agaaaagtac
     4921 atcatattgt cccaaatact ttgccatcaa ggagagtttt tggaatctgt gaagcaatct
     4981 ctgaaataca cctagttagt gaaagtatgg agtttaatga gataatagga cttgaagtta
     5041 atagttatga tgtgattcca aattcatttg tttcaaaaac tattaaggaa ggaaagtatg
     5101 ctatatttac tcatactggt agcttagata atttagacaa aacttatgaa tatatatggg
     5161 gtacttggtt tttaagttca aaagaagaac ttgatataag agatgatttt gaagtatatg
     5221 atgaacgatt tttaggtcct agtaatgtga actcacaaat tgatatttat atacctataa
     5281 aataatctat aagaaaacta aaagataatt tgattaagaa atagatgttc gtaaaagagc
     5341 atctattttt tatttttata agatt
//
LOCUS       DAELTA010000206         5341 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.208, whole genome shotgun sequence.
ACCESSION   DAELTA010000206 DAELTA010000000
VERSION     DAELTA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.208"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            232..903
                     /locus_tag="KPG13_003332"
     CDS             232..903
                     /locus_tag="KPG13_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0987019.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            900..2030
                     /locus_tag="KPG13_003333"
     CDS             900..2030
                     /locus_tag="KPG13_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0987020.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            2450..3295
                     /locus_tag="KPG13_003334"
     CDS             2450..3295
                     /locus_tag="KPG13_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987021.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            3295..4323
                     /locus_tag="KPG13_003335"
     CDS             3295..4323
                     /locus_tag="KPG13_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987022.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            4326..5339
                     /locus_tag="KPG13_003336"
     CDS             4326..5339
                     /locus_tag="KPG13_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987023.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
BASE COUNT         1983 a          537 c          954 g         1867 t
ORIGIN      
        1 agaatagtga caaaataata taattattat tagaaatatt ttagcctgct aacatagaaa
       61 aagttagtag gctattttta tctatgatat caattccatt aagaatagtc taaaaaatac
      121 tatcaaaatg ctataaaaaa tactatcaaa atactataaa aaaatagttg aaaaataaat
      181 aaattaatat aaactaaaat aaagcctaca aaaaagaggt gataaataga aatggctaaa
      241 atactaattg tagaagatga aaaaaggatg caaagtatta ttgtagaata tatgcaaaag
      301 ggaggatata cttgtataac tgctgatgat ggagtcgagg cacttacaat attaaagagc
      361 aacaatatag atttaatgat attggatatt atgatgccgt atttagatgg attttcagtg
      421 tgtagagttt cacgtgaaat gactaatata cctattatta tattgacagc taaaggtgaa
      481 gaggaggaca aattaaaggg ttatgaatat ggcgcagatg attatataac aaaaccattt
      541 agccccaaag tacttttagc aaaagttaat gccttgttac gtagatacac tactgatatt
      601 cccaaaaatt cattgagttt aggaaagatt tttattatgg tagcatcaag gcaagtgtat
      661 gtagaagata aattgattga tttaacatat aaagaatttg aattattgcg tttatttatg
      721 gaaaacccta atcaagtttt ttccagagag aaacttttaa attgtatatg gggatatgat
      781 tttgaaggaa acacacgaac tgtagataca catataaaaa ccttacgcaa aaagcttggt
      841 agtgaaggac accatatagt tacattaatt cgttcaggat ataagtttga ggttaaggaa
      901 tgaatagttt aagaaagatt atagaaaaac tgaaaaatgt agatatttca aattggatta
      961 aacaaaattt tggactatat tcagttagaa aaaaagtgtt tttactttct aaattatcag
     1021 gagcgttaat tatattattt tatctggttg ttgaagaatt ttcgataaac tctaaattag
     1081 aattttggat ttggtttata atactggtta tatttattat gagtattgat ttcttattgg
     1141 gaaaatttat ttcagaacca atcacatcta taaataaatc tgcaaaaagt atgtcccaac
     1201 ttgatttttc taatccatgt actgtaaata ctaatgatga atttggagaa ctttctagga
     1261 gcctgaatac aatgtctaca aatctacaac aggcattatc agacctagaa agtgcaaata
     1321 ttcaacttga aaaggatgta aataaggaaa gaatgctatt ggaacaaaga aaggagttgg
     1381 tagatacaat ttcacatgaa atgaagactc cacttggcat tatacgtgcg tatacagagg
     1441 ggcttatgga tgaagttgat gaagaaaaaa agaaaaatta tatgaatgtt ataatagaag
     1501 aaactgatag aatgaacaat atgattgtat cattattaga cttatctgca ttggaagcag
     1561 gagtttctaa acttaaccca gagcgttttg attttataga gtttgttgaa actgtggcag
     1621 gtcgtctttt aattgatata ccagatgcca atttttattt tacatatgat ttgccagagt
     1681 atgaagtttt tgtagtggca gataaaatga gaatggaaca agtagtagag aatctagtta
     1741 ttaatgcaaa gaagcatgtt gtatataatg gtaatttaga tttatcgatt acatgtgaaa
     1801 atggcttgct tttatttgaa atttataatg atggtaggtt tatagagcaa gatgagattt
     1861 caaagatttg gtctaagttt tatcgtagtg ttcaatctca gcgtacagga ggttcaggtc
     1921 tagggcttgc aattgtttca caaatactta caatgcaagg gttagaatat ggagtgaata
     1981 atcgtgatag aggagtagag ttttatttta tgattccaat tgatgaataa tttagtatta
     2041 atttataaat tataagtatc tgatgtaaaa tatttttctt tgtatatgta agcatttaaa
     2101 gagaataaaa taaaaaaact taatgaaaaa agcttaatga aaaagtctaa taaaaagctt
     2161 aatgaaaagt cttgtttttc tgatgaaaac aagatttttt attaagcttt ttattatttc
     2221 acttcgattt cacaagagag acactctgat ttcaccaaat agaaataaaa taggttaaat
     2281 aaattaaaaa atagaagcgt aattaaataa ggttacaagg gggagtactt atgaaatggt
     2341 atacatggtt gactattata ttgataatat tgctttctat tccgcttaaa attaaattta
     2401 taaaatggtg ggatagacga caaaaaaatg ataaaggagg ttatgataga tgataaaagt
     2461 aggagagtta aaattttctt atggaaaaga taaacagata ttgcatggac ttaattttga
     2521 agtaaaagaa ggagaaatct ttggattcct tggacctaat ggttcaggaa aatccacaac
     2581 acagaaaatc ttaaatggtg ttttaaaagg ttacaatggt cacgtatccc tttttggtaa
     2641 ggaagttaaa gcatacacag aatctttata tcaaaagatt ggtgttttat ttgaatttcc
     2701 ttatttatat accaatttaa gtgcaattga taatttagaa tatttttcgt ctttttatcc
     2761 taaaaatcaa aggagagata tatatgaact cttagatttg ttagagttta agaaagagtt
     2821 tataaataag cctgtatcat cctactcaaa aggtatgaag caacgtatta gtatggcaag
     2881 agcattggta agtaatccaa agttgttatt tttagatgag cctactagtg gacttgaccc
     2941 atctggagct gtactatttc gtaagattat tgaagaagaa cgaaaaaaag gaacaactat
     3001 ctttttgact actcataata tgctagatgc agatttaatg tgtaacaatg tggcttttat
     3061 tgcagatgga aagattatgg taatagataa acccaaaaat ttaaaaatga aaaatagcaa
     3121 taataaagtt gaagtggaat ttgtatataa tggaaatcga gatattgaaa ttgtagatat
     3181 acaagagtta gaatctggaa taacttttaa atatgatgaa atcttaagtg tacattcaaa
     3241 agaacctaca ctagaagaag tttttattaa atacacagga aggatgttag tgtaatgtac
     3301 tctaaatttt ttaccttagt aaaaaaagat ttttggatga tgatttatgg taaatttttt
     3361 gtgctgacac ttggctctct catactttat tcgtgttata ttaattttgg ttatgtaaag
     3421 tttatggatg aagatagtta tcacaataat gtatatctat ataatccaga gaatataagc
     3481 atgagtgcat ctccattaat taatcaagtt tcttctatag gtgaattaaa agcaaaactt
     3541 gatgaagata caagtgcagt ttgtattgat atgagttcca gtaagccgaa agtaatttta
     3601 catgaaagta ttaaaggagt tgacgattat agagctgact atgcattgtc attattatca
     3661 tcagaaaaag accacaatag caaaatatca gacaacaatg caataattca aattaatgat
     3721 aaggatacat ctgagataaa acaaagaaaa gacatgactt gtgagatttt attctttgaa
     3781 attgtagcta ttggattttt agggattgcc tctttattat ttaaagaaaa gcagatggga
     3841 gtaatgcgta ttactggtat tttacctgta aaaagaagtc tttttatatt atcaaagata
     3901 tttgcttttt tgatatgtga cctatgtttt acagttcttt tagtagtatt taatattgga
     3961 ttttttgaag gaataaaaat acttccacaa gtattagttc aagctagtat tttatcaatt
     4021 attatgtctt tgattggaat aggatgctca atgttattga gagattttaa gcaattttct
     4081 cttatatatc ttgtggttgc tgtattcata actacaccta tatttctatc tgcaaataca
     4141 tcttttgaaa tagattggat taaatatcat cccttctatc atgtttatag tcagcttaaa
     4201 aatgcgtttt tcatgaattt ttcaaaagaa ttagtttatt ttgtagtgtg ttctttgaca
     4261 ataatagttc tatttttact tgtaaaatat atttttaata aagagttggt tagggaggga
     4321 taatcttgat aggattacgt taccagttaa agagtataaa aaaagataaa atgtgtataa
     4381 tttccttctt tttaccagtt gttatggcga ttttaataaa ttttatggga acaattgata
     4441 tatcttcatt aggagagata cgttttggta tagtgaagga tacacttcct gtacaaacag
     4501 agcagtggtt gaagaagtat ggtatggtaa gtgtatatga cacaaaaatg catttaatac
     4561 gtgaaattaa ggatattaaa acagacacaa ttggagttgt tagagatggt aaagggataa
     4621 aaactgtgct ttatggaaat gagattaatg ctataaaaaa tgttggaaag acattgccag
     4681 agatgtttag actaaagggt gatttaaaag atgttgaggt tacaatattg cctaaagata
     4741 gtattttaaa agagtttcaa aatgttttta ttgccatgat tttaattact gctatgttta
     4801 tgggatgcac ctttaatgca atgaatatta tatctgaaaa agaagatgga atatcattta
     4861 taaatgaagt tttgccacag agtagaagtc agtatattat acaaaaagta tttttgggtc
     4921 ttttatgtgg gtgtttatct tcaatgataa ctgtatttat atgtttggat atttcaatat
     4981 tagatatgat aatgatgttg ttactcgttg tattttcagc atttatttca tcacttatag
     5041 gtttatttat tggaaagttg tcagaaggtc ttatggtagg aattgtttat attaaaatag
     5101 taatgatttt atttataggt actcctttgt taatatatct tctaggggtt aataccagtg
     5161 gtatagggaa tttatactat ataatgcctt cagttgctac atttaatggt attatggaga
     5221 ttattaatgg taatgtcatt attatcaaag atattgtaat tttggctgta cattgtgtag
     5281 tatggtttat gatatatatt attttagata aaaaaagaaa gttatttttt agtagataac
     5341 t
//
LOCUS       DAELTA010000207         5325 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.209, whole genome shotgun sequence.
ACCESSION   DAELTA010000207 DAELTA010000000
VERSION     DAELTA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.209"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            145..981
                     /locus_tag="KPG13_003337"
     CDS             145..981
                     /locus_tag="KPG13_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0987024.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1334..2911
                     /locus_tag="KPG13_003338"
     CDS             1334..2911
                     /locus_tag="KPG13_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG0987025.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2904..4088
                     /locus_tag="KPG13_003339"
     CDS             2904..4088
                     /locus_tag="KPG13_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG0987026.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4106..4705
                     /locus_tag="KPG13_003340"
     CDS             4106..4705
                     /locus_tag="KPG13_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG0987027.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4729..5199
                     /locus_tag="KPG13_003341"
     CDS             4729..5199
                     /locus_tag="KPG13_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0987028.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1990 a          561 c         1023 g         1751 t
ORIGIN      
        1 gacaataaaa aatattttgt tattgtagta ttatagggta aattgcaaaa tatatacgaa
       61 agtaaaattt taaatattga cattaaatta aatatactat actatgtaag tgtgatgaga
      121 gaattttttt agaggggatt tatagtgaat ataaaaaaga tatttaataa caatgtagta
      181 gtaagctccc tagaagatgg cacagaaata attgtcactg gtgctggagt aggatttaag
      241 aaaaaagtag gcgatttgat agatgaaaat ttgatttcta agaaatattt tgttcaagac
      301 gatcaaaggg acaaatacaa tcaaatactt aataagacct ctattgaata ctttaaaatt
      361 tcagaagaaa ttatagaaaa ggctaatgaa gttttaaata ctcaagtgaa tgattcaata
      421 atattagcct taactagtca catagaattt gctgtccaaa gagaaaaaca aggtataaaa
      481 ttaccaaact taatattgaa tgagacaaaa caactttata gggaagaatt tgaatttgga
      541 ttatgggcaa ttgatgaaat tgaaaaaagg ataggaataa aattgcctga agatgaagca
      601 ggatatattg ctattcacat aatcaatggt ttggaaaatt ctcaaaaaga tgaaggtatt
      661 aatatactag agttttcaaa acaagttatt cagataattg aagaagtaca tggatttaaa
      721 ttggatgtaa attcattaaa ttatactaga cttataactc acttaaagtt ttttgttcaa
      781 aggattctcc gaaaagaaac ttacaatgat agtgatattg aagatatgta taagttagtg
      841 aataaaaatt acaataaacc taaagtgtgt actgaagaaa ttgcagcatt ggtattagac
      901 agatttgaat ataagattag taaagaagaa gaattatatt taatgattca cataaataaa
      961 ataacaaaca gtggttgtta ggattgtaac tgataaagca ggcaaaacct aagtcattga
     1021 gcttaactat tagattaagt tttttgattt aggttttttt attagataca atcaaaatac
     1081 agacagtgac ttttaggatt gtaactggtt aagcaggcaa aacctaaatc attgaattta
     1141 actattaggt taagtttttt gatttaggtt tttttattag atacaatcaa atacagacag
     1201 tggttgttag gattgtaact ggttgagcag gcaaaaccta aatcattaaa cttaattatt
     1261 aaattaagtt ttttgattta ggtttttcta taaatttgat tatgttttta gataaaaata
     1321 ggaggggaaa attatgaaaa aaaataagtt aggtttatgg aacttcttcc aaatgttagg
     1381 aaaaacgttt atgttcccaa tagcactact tagtgtttct gggatgatgc taggacttgg
     1441 ggcaggtttt acagacccta agatgattga aatggttcca tttttaggga atgaatatgt
     1501 taacttagtt ttaaacttca tgttaacaat aggattattt gcatttaaca acttgggagc
     1561 attatttgca atggcaattc cactaggatt attaaaaaaa gaaaaagagt ttggtgcttt
     1621 ttcaggatta gtaggattta tagcaatgca tataggtaca aatttttatt taacaagagc
     1681 tgatttgtta gtaccagcag aacagatgtc aactaatggt caagctatga ttatgggtat
     1741 acaaacatat aatacaagtg ttttaggtgg tattgtagca ggattaattg tttatgcaat
     1801 atatgataaa gttagtaatt taaagatacc agaatcatta ggattctaca gtggaccaag
     1861 attagttcca attgttagtt taatagttat gagtatagtt ggattattaa tacctattgt
     1921 ttggccacca tttttcaatg cattccaaag tttaggaaga tggatttctt cagcaggacc
     1981 attaggatat ttttcatatg cagtggcaga aagagttaca attccatttg gactaaatca
     2041 tcttgtaaca tctgtattcc gttttacacc aataggtgga actgctgtta ttgatggaga
     2101 aacgtacttt ggtacattga acatgtttat ggcatatgta gaaaataatc agattatacc
     2161 tcttgattta gctggtaaaa tggaacaagg taaattgatg attcaatatg gattagctgg
     2221 tgctgcattg gctatctacc gtacagcaaa accagaaaat agaaaggcac ttaaaggact
     2281 tttaatatca ggagtgttga cagttattat aggtggtata agtgaaccaa tagagtttct
     2341 attcttattc atatcaccag cattatttgc tttccataca tttatgaatg gtctagcaaa
     2401 catggtactt ccatacttag gcgtgttgat gggattcaca ggagatttaa ttgcatttat
     2461 aagttttggt gtgttaagag gaacagctac aggatggcca atagcagtat tagtagcagt
     2521 tttatatttt gcaatatact attttgtatt taagtgggca attattaagt ttaatataaa
     2581 gaccccagga cgtgaggata aagaaataaa agtatctgaa ggaaatggtt caaatttcaa
     2641 aaatttgtct acatataaag gacaacaaat gatatctgct ttaggtggtc aatctaatat
     2701 attatcacta gataattgtg taactagatt acgtttaaaa ttacaagatg tatcattaat
     2761 aaatgaggat gcaattaaag aggctggtgg tatagcagtt gttaaattgg atgagcatac
     2821 aatacaagta attataggaa cccaagtgta ttctttaaga aagcagatgg ataaagctat
     2881 ggaaggtcat ttggaggcat gtaatgatta attttgataa aaaagtagac cgattgggta
     2941 catattgtac ccagtgggac tatgtagaag atagatttgg taagaaggat ttgcttcctt
     3001 ttacaatttc agatatggat ttagagtttc catttgaaat aacagaggtg ttgcaggaaa
     3061 ggttaaatca tagagttttg ggatacagta gatggaaaca tgatgatttt agaaattcag
     3121 ttacgaattg gtatattaaa agatttaatt gtagcgttga tagtaactgg atatattata
     3181 gtccaagtgt aatgtattca attagcaaac ttattgagat gttaagtaat gaaggagatg
     3241 gaatccttat aaatactcct gcatacaatg ctttttatga tgtgataata aataacaatc
     3301 gtaatattat aaagtcacat cttgtaaata ggaatggatt ctattcaatt gattttaatg
     3361 attttgagaa aaagtgtaaa aaatctaaga tatttatttt atgtaatcct cacaatccaa
     3421 ctggtagagt atggagtgag ttagaacttg ttaagatgat aggaatttgt aagaataatg
     3481 atgtcaagat aatatcagat gatatacaca tggatattgt atatgacaat agaatgacac
     3541 ctatcttaaa tgttgcaaag gactatttag attctgtatt catttgtaca tcagcgtcta
     3601 aatctttcaa tataccagca ttgactgggt catatgtgat tataccaaat gaaggtatta
     3661 gaaataaatt tgaaaatatc actagatacc gtgattttgt aaattcgcca gcaattctag
     3721 caatattagc cacaatggtc tgttatgatg actgcgaata ttggttagat gaactattaa
     3781 aataccttaa agagaatctt aaatatacga ttgattatat aaaagataat ttagaaccat
     3841 tgaaattggt catgccagaa ggctgttact ttgcttggat agatttttcc gaacttaaaa
     3901 taagtggtga gaaattccaa aaattattga tggatattgg agaagttgct attatgtcgg
     3961 ggtctgtata tggtgaagaa ggtgagtttt atttaaggtt aaatgtggca tgttcaagag
     4021 aaaaacttga agatggttta aataggatta aaaaaactat ggattatatt gaaaaaagaa
     4081 ttttatagta ggaagggaga tagttatggg atatttaaaa acaatggttg atgttatgac
     4141 tactgaaatg aatgccataa gatgtttaat agatgaagca ggaatagaat atgaaagtat
     4201 agttagtgaa attgcaaatt gtgaaggcaa agtaattttt atgggtgtag gaaaatctgc
     4261 acatataggt aagaaattgg cagctacatt tgctagtact ggtacaccta gcttttttgt
     4321 acatgcaaca gaggcagtgc atggagattt aggaatgatt gaaagtaagg atattacaat
     4381 tttaatatca aatagtggaa attctatgga agtggtaaac tgtataaaat atattaaagc
     4441 aataggttct aagacaattg catttacttc aaacagaaat tcagtactgg caaaagagtg
     4501 tgattatgct ttaatatatc ctgcaaagga tgaagcagac catttaaatc tagcaccaac
     4561 aacatcatct acaattactt tggttttagg agattctatt gcatgtgcat tatctaagag
     4621 tagtaatttt ggttcttcag atttttataa gtaccatcca ggaggaagtt taggtgagaa
     4681 gttaaaaaca gctaacaatg gataaaagca aaaaggagag tagtttttat gttcaaaatg
     4741 tttaaaaaaa aatcaataaa atcacccata tcaggaaaag taatagagtt atctaaaata
     4801 caagatggtg tattttctca aaaaattatg ggagagggtg tagctataga ttctacagga
     4861 gacattgtat atgctccaat caatggaaag atagtagtag tagcagagac taaacatgca
     4921 tttgtaatag aattagaaaa tggaatggaa cttcttattc atgttggttt agatactgta
     4981 aatttaaaag gcgaaggttt tgaggcattg gtgagtatgg gagaacaagt gaaggaagga
     5041 acaccaatgt tgaaaattaa ccgtcctttg attgaatcaa agggtatatc acttattact
     5101 ccagttacaa taactaatca ttcagagtac aatatgaata catgtaatgt aggcaatagt
     5161 gttgaaggtg ggaaagatac tgtaattgag tttaaataaa gaactaaaat acaaggttaa
     5221 tatttataaa tacctataaa cttatgtttt ataagattga ttgtatagat ttgttgtata
     5281 aaaaagaact ctttaattat aaattaagga gttctttttt atttt
//
LOCUS       DAELTA010000208         5237 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.210, whole genome shotgun sequence.
ACCESSION   DAELTA010000208 DAELTA010000000
VERSION     DAELTA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.210"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            337..1197
                     /locus_tag="KPG13_003342"
     CDS             337..1197
                     /locus_tag="KPG13_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG0987029.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1187..3058
                     /locus_tag="KPG13_003343"
     CDS             1187..3058
                     /locus_tag="KPG13_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG0987030.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3078..4532
                     /locus_tag="KPG13_003344"
     CDS             3078..4532
                     /locus_tag="KPG13_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG0987031.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         2025 a          533 c          949 g         1730 t
ORIGIN      
        1 ttcataatag taaaatagat aatggttcag acaatattat aaatactagt gacggtaaaa
       61 tagtatcaat ttagtatata aaccttgtca gataatcgca taacagagct tgtagttata
      121 gaataaaaaa atatctagga aaccgttgac agatttatat ggatagtata acatatagaa
      181 tatgaaatta aacaaaaaag aattttaatt atggcgacgt agtcataatt ttgagtatgt
      241 tactgattag caggcagaac tcaagtatat atgtgaaaaa tagagggata ctctatacat
      301 atatgcttga gttcttttta ttgaagggag gaatatatga aaataattca gattttaaat
      361 aataatgtag ccttagtaaa aagaggtaaa aatgaaatta ttgtatttgt taaaggaatt
      421 ggctttaaaa aaagagtggg acaagtaata aatgaaaatg aaatagagag aacatatata
      481 ctagattcat acgatatgct agaacacttt agttatctac tacaaaactc agactccaat
      541 aatatagttc ttattaatga aataattgaa tatggtgaga aaactttaaa tataaaggca
      601 agtgattatc taagcttaac cttactagac catttagagt ttttacttaa aagatgtaaa
      661 aagaatcaat ttataaaaag tcctttggta tggaatgtta aaagatttta tccaaagcat
      721 tttgaaattg gaatgcatgc actaaaatta attggtgata gtaagggaat taatcttcct
      781 gatgatgagg cagtatcaat agcacttcat tttgtaaata tggaggtaaa taaagagtct
      841 catgatagta caattgtaga gttgagaact ttagcagata tagtttctat aataaaatat
      901 cattttaatg tagagctaga tgaaacttca acaaattata tgagatttac tactcatcta
      961 cagtacttta tccaaagaat tatgtcagag gaagcttcaa tagatgaaga taattctata
     1021 gatttatata aacaagtaag tcaactttat ccaaagtcat ttggaagtgt tcaaaagata
     1081 agaaaatata taaaatcaca atttaatgtc gatatatcta taagtgaaga aacttattta
     1141 atgttgcata taaatagggt gactcaaaga ctggaggcaa aatcagatga aatataaaga
     1201 tttaaatgaa aatataataa aacttatagg gggaaaagaa aatataaagt cggtagcgca
     1261 ttgtgttact cgtttaagat ttactttaaa tgataggagt atcgctcaaa cagaagaaat
     1321 taaaaaatta aaaggagtaa ttgatgtagt ttctaatgat gttgcttatc aaattataat
     1381 aggtactcat gttgtagatg tatataacga gtttatgtct atgataggtt taagttcaag
     1441 tgaatcaagt ataaaaagtg aaagaaaaaa gggaattaga ggtatcaaag gtatatttaa
     1501 ttccatcttt gtggtagtct cagagactat gacttcagta attgaagtac tacttgcagc
     1561 tggtatactt gcaagtatat tagcactttt aaatatgtct ggcatacttc agagtgacag
     1621 tcctacatat caaatattag atacattaag aagtgctaca tttcacttct taccagtatt
     1681 aattgcaata gcatcagcaa aaagacttaa tgtaaatcca tatatagcag tagtattagc
     1741 agttacgtta ttatcttcat caatagatgg tgtagaagga ttaagtttat ttggtataaa
     1801 tttacagtct acaacttatg caaacagttt tattccaata cttttaggag tatgggcaat
     1861 gggaattata attgaaggtt tgaaaaaaat attacctaaa gcactacact attttttagt
     1921 tccagtatta tctttaataa taactctatc agttacatta actgtatttg gacctattgg
     1981 tatgtggatt ggtgatggat taaactatgc atgtatgttt ttaattgata cattgggtaa
     2041 ttggagtgtt gttgcactgt atgcagcttt acaaccactc ttaatagtct ttggtgcagc
     2101 aaactttact atgcctatat ctttaaattc tgtgacaaca ttggggtatg acccaatttt
     2161 tatgggcgct gcaacaatat ctgatattgc agtttgtggt gcaatgtttg gatatttctt
     2221 aaaaactagg aataaagaac aaaaacaatt atttggtaca gtaagtttta gtgcacttat
     2281 gggagttaca gaaccttcag tgtttggtgt gtttatgaaa tatagaagac catttgtagc
     2341 agtaatgata ggtggaggta taggtggttt aatagcaggt ttagctcaag ttaaaacata
     2401 tggaatggtt tggggattag ctgcattacc aacgtatctt gttggaggag aggtatctaa
     2461 ctttacattt atgataataa gtgtagtagt atcatttgta gcagcaacag cagcttccta
     2521 tttactagga ataccttcag atgaaaaaga agaagttaag ggtgataatg aaaaagaatt
     2581 agaactaata atcaacgaga acaattcaag aactattagt attggaaatg ttgcaaaagg
     2641 tcaggtaatt gcacttgggg aagttaatga caaagcattt tcctctggag cattaggtaa
     2701 aggggttgga gttataccta gtgaatcaaa atcaactgtt atatctcctg tagatggaac
     2761 tataactata gtatttccaa ctaaacatgc atatggtata gtgactgatg aaggattaga
     2821 aatattaatt catattggga tagatactgt aaacttagat ggaaaattct ttgaaagtct
     2881 tgtaacacaa aatcaaaggg ttaaaaaagg agatgcttta gcagtatttg aatctgataa
     2941 aattatagag gaaggctttg attctactat aataactgta gttacaaata ctagcgatta
     3001 tttagatgta atctcatcaa atgatgaatc agaagaatta ttaactgtaa tagtataaat
     3061 tttaggaggg aaaattcatg aatattaaag atacattttt ctggggagga agtattgcag
     3121 cccatcaatg tgaaggttca tgggatagtg acaacaaagg acctgcaatt atggattttg
     3181 taactaaggg ctcttatgaa atgccaagag taattactga taaaatagaa gaaaaattgg
     3241 attatccatc tcataatgga atagattttt ataatagata taaagaagat atagctttat
     3301 ttaaagaaat gggatttagt gcattaagaa tatctataga ttggtcaaga atttttccaa
     3361 atggagatga tgaaaatcct aatgaattag ggattaaata ctatgaaggt ctgatagata
     3421 cattaataga aaataatata gaacctattg taacactata tcattttgaa ttacctatga
     3481 atttagtaca taaatatggt tcatggaata acagaaaact tatagatttg tatttaaagt
     3541 acagtgagac tgtaataaga agatttgatg ataaggtaaa gtactgggtt acatttaatg
     3601 agatgaacca tattgaccct caaacagagc actcggatat ttttacttat atgatagcag
     3661 gtattaagta tagtgaaata gaaaataagg ttcaaacatt ggcaaatgta gggtataata
     3721 tgactttggc gggtgtaaag gttgcaaagt tggctagaga aatcaatccc aacaataaag
     3781 ttggatgtgt atttggacta aatccaattt atccatacaa ttgtaatcct agtaatgtgt
     3841 taaatgcatt tttagataat gaccgtgatt attatcaaat tgatgcaatg tgcaatggga
     3901 agtttccaca atataaatta aaagaatatg aaaaaaataa tataactatt gaagtgactg
     3961 atgaagacaa aaaagcattt aaagaaggta agttagattt tataggactt aattattatt
     4021 tttcaagtgt atctactcca aaagaaaatt tatcaggtga taaatcttta tttggtggga
     4081 ttcaaaatcc atatttggaa caaagtaagt gggggtgggc aatagaccct ataggtttaa
     4141 gatttacctt gaactatatt tacagaaagt atcaattacc tatcttaatt acagaaaatg
     4201 gtttaggggc atatgataaa atcgaagaag atggaactat aaatgatgat tatagaattg
     4261 aatatttagg taagcatcta tctcaaatga agtctgcaat tgaagaagat aatgttgaat
     4321 gttttggata tttgatgtgg ggaccaattg atttagtcag tgcaactaca ggtcagatga
     4381 gtaagagata tggatttatc tatgttgact tagatgatga aggcaaagga agttttaaga
     4441 gatataagaa aaaatcattt gattggtaca aagaagtaat aaaatctaat ggagaagttt
     4501 tagatttttc tacggatata gtagtagggt aggaataaga taaatagaag ttaggaacta
     4561 atgttaatta gtgtctagta aagcatttaa aatagagtat agggtcttta aagtaaagtg
     4621 tgtattcttc ttaaaagtaa tggcaactaa aattactata aaaaggagaa tacacatttt
     4681 ttatgtttat acttaagtaa atatttagac atattcatag tataatttac tcattatata
     4741 aaaatagtat gatttactca ctatataact ttataaattt tctggaaaaa tgtcagaata
     4801 tattgacaaa gaaaagattt aaaattataa tctatattaa gaatagtttg acaatataaa
     4861 aacaagtatt tttataaata gtgaaattat ttttattgtc aatttaagaa taatttagga
     4921 ctactataaa gataaaggta tagatgtcat tatatgataa attatacttg tatagaaaga
     4981 attttaaagt atggcttatc attgatttaa tataataaat aatttttgtt tctagtaata
     5041 gttcctagga ataagatatc taaattcgtt tagaaatagt ctaatttaca aaggagggga
     5101 tgcactaata gataaaattt ctatataaag aagacttaaa gtgaagtata ttctgataat
     5161 attggtttaa tgggtatatt attatttcgt tttagatatt ttaagaataa tttaagacgt
     5221 ttataaaaat gtattct
//
LOCUS       DAELTA010000209         5211 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.212, whole genome shotgun sequence.
ACCESSION   DAELTA010000209 DAELTA010000000
VERSION     DAELTA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.212"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(405..1811)
                     /locus_tag="KPG13_003345"
     CDS             complement(405..1811)
                     /locus_tag="KPG13_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0987032.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1856..2563)
                     /locus_tag="KPG13_003346"
     CDS             complement(1856..2563)
                     /locus_tag="KPG13_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0987033.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2734..3648
                     /locus_tag="KPG13_003347"
     CDS             2734..3648
                     /locus_tag="KPG13_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987034.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3648..4409
                     /locus_tag="KPG13_003348"
     CDS             3648..4409
                     /locus_tag="KPG13_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987035.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4421..5158
                     /locus_tag="KPG13_003349"
     CDS             4421..5158
                     /locus_tag="KPG13_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987036.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1791 a          809 c          686 g         1925 t
ORIGIN      
        1 agctattata actacacaga acagcttata attaaaactt ttttaggatg tcattatatt
       61 ttttcttaaa attaaaaaac actataccat aattctattt tagtagaatt tttttgtcaa
      121 taataaaatt aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata
      181 accttacaat aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag
      241 ccataagcgc ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat
      301 ttatccttat tctactctaa tagagttaat ttgtcaataa agaataaaat tattttcaaa
      361 atattttcat tcttcattct tatctgttat tattttataa aatatcactt aattagaggt
      421 aattttagta ttgtttttag tccctgacta tcaatgctcg taatatctaa tttacctcta
      481 tgtgcattaa taatgccaga tactatcaaa atacctagac catgattttg acctttggca
      541 tatgcctcac ttagtccatt ttggctcttt aataaactat taattctttc ttgactcata
      601 ccttttccat catcttcaac aattatatag caaaaatcta aatcagactt agaggtaacc
      661 cttatattac atccttgttg gttatgagtt atactatttt gtattaaatt actaatagcc
      721 ctttttaata attttttatc tgcatctata ataatatctt catttagtat atcaagttca
      781 acgtaaaagt cattatccaa attattattt ataaactcag aaataagctc tctcaaaaac
      841 gcagaaactc taatcttatc acaattcaat gtttgcatat tatattcaag tttagacact
      901 aaatttaggt catttactaa ttctctgatt ttaatccctt gattgcatat aatagacact
      961 tgctcttgtt ctctatttga aaggctactg ctttcttcaa gttctccagc atatccaatt
     1021 atcattgaaa gtggagttct tatatcatga gatattcctg caatccagtt agacctagct
     1081 tcctctttct tcctaagttt ttcatctttc atttgcaact ctttagatac agtattaata
     1141 gattttgaaa tatcacttag tattcctttt tcttgtaaaa gtactggttc tccttttgac
     1201 ataagcttta tgccagtaat aattggtttt atagatttca ccatttttaa acctatgaat
     1261 agagctatta aaaaagtaac tattgcatta accagaaaca tacctattat taacatagga
     1321 aattgttcta tttccttaac cgtataagta aggttaaact tagtatactt gtcttttgga
     1381 taacctgtta ctaatattcc atttggatgt tcccatacat acacaggata gtcctttaaa
     1441 aagaaccttg aaaactttgc tacatctgtc aaagtataat gacttggaat ttcttttggt
     1501 aaatcaaatc cccatattct atctccattg ttatcaatta acattgccca aactttattt
     1561 ttatgtagta cttccttact agaatcatct aaaataaact ttccatctct taaaatcaaa
     1621 ttatcttcaa tcataatcaa gtttgatttt atttctctac tatcacttgt atctaaatta
     1681 tcagagtatt tagatacaaa taacatatat gaaaacagaa gaaaacctgc tatatttaat
     1741 acaataaaaa gtaatgaaat caagatactt gaacctataa atcgcctcaa tatgtacttt
     1801 acactatcca aaattaaatc actcctcttg atttctttta ttctaatatc taaaattaat
     1861 taatcatcaa cttatatcca agacctctta ctgtaataag atgtttaggt gtagatggag
     1921 ttttttctat cttctctcta acccttctaa catgaaccat aagggtattt tcatatccat
     1981 acatatcatc tccccaaatg gcttgacata gtgaatcatt agtaacaatt ctccctttat
     2041 tttcatacaa ctttaaaagc aacgaatgct ctttagctgt taaagaaatc tcttgcccat
     2101 ctttctcaac acatgcacta tttaaattca caacaacaga atctcccagt ttaaatactg
     2161 gcaactcctc ttcttctata ggcacatata cacgatttaa tatacccaca agccttaaag
     2221 taagttcctt tggtaaaaat ggctttacta tataatcgtc agcccctaag cccaaaccaa
     2281 tcaatctatc ttcatcttca cctcttgcag agagaaaaat cactggtatt ttagatgtct
     2341 ctctaatttt tctcataata gaaaaaccat ctccatctgg caatacaaca tctaaaattg
     2401 cacaatctgg ctttacattt ctaaataccc taacagcttc ctcataactt gatgctaaaa
     2461 atactctact aaatccctct tttctcagga tttcatctat catttttaat aattccttgt
     2521 ggtcatcaac cacaagtatt tttttatctt ttattgactc cataatcatc accctccttt
     2581 agtataccat atacccattt tcacaacttt gcctaaatag acttttaagg ttattgtaag
     2641 gtaaatttca gctaattgaa atgtaaccaa gttattatac tgatataagt taaaagaatc
     2701 aataagcaca gattaaaaaa ggggatgaaa catatggaaa tgataattag aacacaaaga
     2761 ctttcaaagg aatacaataa aatatttaga gtaaaggata tagacctaag agttccacaa
     2821 ggagctgtat atggtttttt gggtcctaat ggagctggaa aatctaccac tcttaaaatg
     2881 cttcttggac ttgctaaacc cacacaagga aatattaata ttttaggtaa agagttaaat
     2941 gaaaaaaata gaatatcaat tttaaaagat ataggttctt tgatagaatc tccatcatac
     3001 tatggtcatc ttacagggct tgaaaacatg accataatgc aaagactctt agatttacct
     3061 aaaaaaaatg taaatgaagc attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa
     3121 gttagtcaat attctttagg tatgaagcaa cgtcttggca ttgctatggc aataatgaag
     3181 tttcccaaat tattaatact tgatgaacct acaaatggac tcgacccagc agggattgag
     3241 gaaattagag aattaataaa atctctacca gaaaaatacg ggatgactgt tctaatctct
     3301 agccatcttt taagtgaaat tgaccaaatt gccacatcaa taggaataat aaatcatggc
     3361 gaactggttt ttcaagatag cataaaagaa cttcataata gaggtcaaag tcagattgct
     3421 atcaaaacta agaatgttgt atctgcacaa aaactattaa atactaaagg atatatgtca
     3481 cttatacaag atgggtactt gactattgaa aatttaaaag actcagatgt cgctaaaata
     3541 aatggtatgc ttgtccattc aaatatagat gtatttagaa tagaagaaca taaaaaaagc
     3601 ttggaagata tattcttaga acttactggt aaggcggtga ctttataatg aaataccttg
     3661 gtctagaatt ttataaactt aaaagaaaga agattattct gatgaccttt ttatttgcag
     3721 ctgttgaaat aatgtggtgt atcgtaaatt caaatagagc tattacacgt aatccagata
     3781 tgttaggtgg atttgaatat tcctatatgc ttatgagttt tgcaagttta aatggtctat
     3841 tctttcctat cttaatatca ataattacat cacgtataag cgatatagaa cataaaggag
     3901 atacatggaa gcttttaaaa tcttctgtga catctttaaa tagtatttat ttatctaaat
     3961 ttttatgttc tgctattttg gtatctatac ctgtattgat gcaagtgtta tctattgtgt
     4021 tgtttggaca ttttagaggg gttattgagt caatttctgt atctttactt ttaaaataca
     4081 cattaggaac aattcttgta agtattgcta tcattgcact acacctatgg attgcaactg
     4141 taattccaaa tcaaatgatt gcaattacat ttggcatgat tggtagtttt ataggtttga
     4201 catcaggttt gttttatcaa ggtattcgta gattgtttat atggtcttat tacctagagt
     4261 tatcaccttt aagttatgct tatgataatg ttttaggttc atctgtctat agaattgata
     4321 tgaatttttc tattacgatt ttagttttct taattggaat gctaatttat tatattggaa
     4381 gaaatcattt attaaaaaaa gaagtatagg agggatttaa atgttaagga agtcagtttt
     4441 gggagaaatt ctaaagttaa aaaattcttt tatatggtat gtattattag ctttgccact
     4501 tataagtgtg ttgattggtt ctggtaattt ttatctaaat caaggcatac tcaaaaaaga
     4561 atggtacagc ttatggactc aagttagtct tttttatggg gaatttttct ttcctatctt
     4621 aatcgcaatc ttttgtgcat atatttgtag gcttgaacat atgaatcata attggaataa
     4681 tgtaatgaca ctgccagtta atttaaaaaa tatcttttta tctaaactta cagtagtttc
     4741 aatacttaca gctattacac aagttttctt tgttatcttt tatatagttg caggaaatat
     4801 gtttggtttt tctggtagtg tacctaaaga attgactggt tggattgcta aaggttggat
     4861 tatttctatt tcaatagctt ctatacaatt atacatgtct ataaagatta gaagttttgc
     4921 aactcctatt ggtattagtc tttgcctttc tctatttggt atgggattaa tagtagcaaa
     4981 acctgctgtt ggtatatttt tcccttattc tatgttaggt agtgctatgg gggttataaa
     5041 gcaatctgct ttaaatgtta atgacactat agttacaata tgtgttgcat ttattactac
     5101 tatagtattt tctgaaattg ccaatagaaa ttttagaaag aatgatgtta tttcttaatg
     5161 gtaaatgcta gatatttttg ggtttgaaat ttatattctt tttagttgaa a
//
LOCUS       DAELTA010000210         5187 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.214, whole genome shotgun sequence.
ACCESSION   DAELTA010000210 DAELTA010000000
VERSION     DAELTA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.214"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1363
                     /locus_tag="KPG13_003350"
     CDS             98..1363
                     /locus_tag="KPG13_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0987037.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            1378..2664
                     /locus_tag="KPG13_003351"
     CDS             1378..2664
                     /locus_tag="KPG13_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG0987038.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            3058..3804
                     /locus_tag="KPG13_003352"
     CDS             3058..3804
                     /locus_tag="KPG13_003352"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG0987039.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            3834..4769
                     /gene="rsmH"
                     /locus_tag="KPG13_003353"
     CDS             3834..4769
                     /gene="rsmH"
                     /locus_tag="KPG13_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG0987040.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
BASE COUNT         2115 a          429 c          966 g         1677 t
ORIGIN      
        1 aattaaatag aaggttttta tatgtgagta tatgtaatta aaaaataaat aaatttcaat
       61 tttaaaattg atgtttaaat aaaaatagga ggtacatatg gttaatacta aaaaaataat
      121 aaatttaggt aataatatta atcttacttt gataaaaaca gaaaagttta aatcgaatct
      181 tataagttta tatgttcaga gactattgga tgaaaaagaa actacaaaaa atgctctaat
      241 accatcaata atagcaagtg gaagcgctaa atatcctagt gctagagcaa tatccaataa
      301 attagatgat ttatatggtt catctatggg agctgatgct gtaaaacgtg gagaacgtca
      361 agtattgagt tttaaagtaa ttaacataag tgaaaagtat ttagatgaga gtatatttga
      421 agaagtagta gaatttttta atgaagtaat aaataatacg cttgttgtag atggtgggtt
      481 taaagaagaa tatttaaata ttgaaaaaga gaatttaaga gaaaagatac aatcaataat
      541 aaatgataaa aaggaatatg cgcaagataa atgtattgag gctatgtgta aaggagagag
      601 atatagtgta agtgaatttg gttatgaaga tgaaatagat agtataactt caagagagtt
      661 atatgaacat tataaaaaca tacttaaaac atcacctata gatatagttg tagaaggtaa
      721 ttttgatgaa gataaagttg tagatataat aagtaaaaat ctaaaatttg aaagagaaga
      781 aattataaat ataccaagag cagattttat aaaaaatgtg gatgaggtaa aagttataga
      841 tgaacaaatg gaaataactc aaggtaagtt agttatgggt tatagagcaa atgtggatta
      901 tgcagacata gataaatatt atgctttagt agttgggagc aatgtcttag gtggtggtcc
      961 acactctaaa ttatttgtta atgtaagaga aaaagaaagt ttatgttact atatattctc
     1021 ttcaatagag aagtataaaa gtatcatgtt tatatcatct ggtatagaaa ctaaagatta
     1081 tgataaagct gtagaattga taaaaaaaca agtagaaagt gtaaaagcag gtgatatatc
     1141 tgatgaagaa ttagaaaaca gtaaacttgc tttagttaat tctatgaaat ctataactga
     1201 taatataggt ggtatgtctg attttgtttt ctctcagtct atggcaaaga caaattctga
     1261 agtacaagac ataatatcta gtatagaaaa ggttacaaaa aaagatatag ttgaagctat
     1321 aaaaaatata gagcttgata ctgtatactt cttgagaaat taatggagga gatgtctatg
     1381 gaaaaaatag ttaatgatat attgaaagaa gaagtttatt atgagaaatt gcaaaatgga
     1441 ttagatgtat attttatgcc taaaaggggt tttatgaaaa agtatgctat tttggctact
     1501 aattatggtt caaatgactt agaatttgta ccaattggag aagataagaa aattagagtg
     1561 aatgaaggta ttgcacattt tctagagcat aagatgtttg agcaacctga tggaggagat
     1621 gcatttgata aattttcaaa attaggagta aatgcaaatg catttactaa ttttacaatg
     1681 acagcctact tattttctgc tactgaaaat ttttatgaga gtttagaaca tctaattgac
     1741 tatgttcaaa caccttattt tacagatgaa aatgttgaaa aggaaaaagg tataatagca
     1801 caagagataa aaatgtacaa tgatgaccct gattggaatg tatactttaa ttgcttgaag
     1861 gcaatgtatg taaattatcc tgctagaatt gatatagcag gtactgttga tagtatatat
     1921 aagataacta aggaagagct atataaatgt tataatactt tctataatcc aggaaatatg
     1981 gctttatttg ttgttggtga cttagatgta gaaaaagtta tagatgtaac taaaaagtca
     2041 aacaattaca aagtagacag actctctaag agtatagaaa gattttatcc agaagaacca
     2101 gaaagtgtaa aagaaaaaga ggttatagaa aaattcccta tatctatgcc tatgttcaac
     2161 ataggtttta aagatagcaa tgttggttta aaagggaaag aacttttacg taaagagatt
     2221 gtaactgata tacttgtagg gatgttattt aaaaaaggaa gtaagcttta tgaagatttg
     2281 tatatgcaag gtttaataaa tgaaaatttt ggagctggat tttcgtctca agtggattat
     2341 gcttttagca taatagctgg agattctaaa gagcctaaaa aagtaaaaga aataattctt
     2401 gattatatag aaaaatcaaa aaaagaagga ttatcaaaag aagagtttga gagaactaaa
     2461 aagaaaaaaa tagggtcatt tattaagtgc tttgattcta taaactttat aggtaatagt
     2521 tttatatctt atgtttttaa ggatataaat ctattggatt atttagatat tattaaagat
     2581 attacttttg aggaagttga agaaagatta aaagagcatt ttaaagaaga gtattgtgtt
     2641 atttctattg tagagcctaa atagaaactt aatcataata agaataacta attaagtatt
     2701 agtttatgtt tttaaatgtt tattttggta aaacaaattt ataaaatata tttatataaa
     2761 ttgaggtaaa aatttaagag ctatagataa gaattagaag aggttgatgt atatatgcat
     2821 caatcttttt gattaaaaaa tatctatgta cacttgtaac tttagtcatg agagatttag
     2881 tttaaaaaat tttatagtaa ttagatataa tatagtgtag ggagttgtag aataaatttt
     2941 aatatttttt gtagaattaa cattaatgtg ttatcataat ttagtataat tacaattaat
     3001 tgtaattata gcatattgct ttggaaatat tttcaattaa tacaaagagg tgacgttatg
     3061 gatagagtag catttacact gtttggcata gacatcatgt ggtatggaat acttatggca
     3121 tgtggaatga ttttaggaac tttaatagca ataaaagaag caaaaagagt gggaattaaa
     3181 gaagatgatg tattaaatat agctataata gcaattccag ttggactaat ctgtgcaaga
     3241 atctattatg ttgtatttaa ttggagttac tatgctcaaa atatgtctca aatttttaat
     3301 tttagaggtg gtggacttgc tatacatgga gggttaattg gtggaatatt agctggttat
     3361 atatacacta agattaaaaa tattaacttc ttaaaaatgg ctgatacagt tatacttgga
     3421 atgcctttag cacaagctat tggaagatgg ggaaatttta ttaatggaga agcacatgga
     3481 ggtgctacca atttaccttg gggaataatg gttgatggag taaaggtaca tccaactttt
     3541 ttatatgagt ctatatggga ttttggaata tttatagttt tattactatt tagaaaaaat
     3601 aaaaaatatg aaggtcaagt tatagttact tatataactt tatattcaat aggaagattt
     3661 tttatagagg gattaagaac agatagttta atgttaggac cacttagaat ggcacaagtt
     3721 ataagtctaa ttggagttat tggtggcata attgcacatg tttatttatc aaagaaaaat
     3781 aaaaataata ttagtgaaga atagtttaaa aagtgaattt aagggaggta actatggaat
     3841 ttcatcatgt atcagttctt ttaaatgagt gtatagagaa tttaaatata aagccagatg
     3901 gcgtgtatgt tgactgtact atgggaggag caggacattc taaagaaata gtaaaaaaac
     3961 tgtcagataa gggattattt atagggtttg accaagataa aaatgctata agcacagcaa
     4021 aagagagatt gagtgaatat gaaagtagag tgaagtttgt tcacagcaat tttgaaaata
     4081 taaaagagga attagaaaaa ataggagttt acaaaattga tggtgtatta gctgatttag
     4141 gagtttcatc tcatcaactt gatgaagctg atagaggatt ttcttatatg caagatgcac
     4201 cacttgatat gagaatggat gttaggtgtg agttttctgc atatgatgtt gtaaatactt
     4261 atacagaaga tgaattaact aagataataa aagattatgg agaagataac tgggctaagc
     4321 gtatagctaa atttatagtt gaagaaagag caaacaaacc aatagaaact acaggagaat
     4381 tagtagatgt aattaaaaaa gctatcccta agaaagccag aatagatgga cctcacccag
     4441 ctaaaagaac ttttcaggca atacgaatag aagtaaataa tgaacttgga gtaattacaa
     4501 agatgataaa tgatgcttct tctataatga atgaaggtgg aagaatatgt ataataacat
     4561 tccattcatt agaagataga atagtgaaaa atgcatttaa gcatttagca tcagattgta
     4621 tatgtccaca gcatttacct atatgccaat gtgataaaga atctgaggtg aaaataataa
     4681 ctagaaaacc tatattacct agtgaggaag aaatagaagt aaatccacgt tcaagaagtg
     4741 ctaagctaag agtagcagaa aaaatataag tagttataag gctaaagaat aatcttatat
     4801 ataagattat tataattaat taaaaagtat tatatatttt gttgcactgg taaagcaatt
     4861 aaaaaacaat taacactata agaaatgttc tttgttattg agatatataa tgaaaattta
     4921 gatataaaaa tatagatata gatggtatta agtaatataa tggagaactt aggaatcaga
     4981 gagtagatag ttagttacat attatattta tggaagaaat tatctaaaaa tactagaagt
     5041 gataaaaata aatagaaatt aatatctaac ctatagattc atataaaagt ataagtgaat
     5101 ttataggttt ttgttgtgta tagaaaaaaa tacaattaga ttgtgtaata gtgtattaat
     5161 ttagcgatga aattttagag gaagaaa
//
LOCUS       DAELTA010000211         5113 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.215, whole genome shotgun sequence.
ACCESSION   DAELTA010000211 DAELTA010000000
VERSION     DAELTA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.215"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            249..1025
                     /locus_tag="KPG13_003354"
     CDS             249..1025
                     /locus_tag="KPG13_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG0987041.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            1041..1406
                     /locus_tag="KPG13_003355"
     CDS             1041..1406
                     /locus_tag="KPG13_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0987042.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(1582..2331)
                     /locus_tag="KPG13_003356"
     CDS             complement(1582..2331)
                     /locus_tag="KPG13_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG0987043.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            2809..3234
                     /locus_tag="KPG13_003357"
     CDS             2809..3234
                     /locus_tag="KPG13_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0987044.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            3266..4453
                     /locus_tag="KPG13_003358"
     CDS             3266..4453
                     /locus_tag="KPG13_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG0987045.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            4645..5049
                     /locus_tag="KPG13_003359"
     CDS             4645..5049
                     /locus_tag="KPG13_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG0987046.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         1994 a          560 c          814 g         1745 t
ORIGIN      
        1 taaaattgtt aagaagtatg ggaagtaagt gcaatttact ttgtaataaa agatgaaaaa
       61 ggcaagctta ggttttaagc ttgtcttttt tattatgtat aaactatgat actttaaaat
      121 tctatgaaaa atttatttaa tagagaaatt aaaaatttaa tggtaatcaa attctttacc
      181 aatgaatata aaaaatgtct acgtgttata atagtttaaa ctaataaacg aaaggtagat
      241 gaatgaatat gaaataccaa catataatag aagttgacaa agaactttgc attggatgtg
      301 ggctttgtaa aaatgattgt ccagtaaaca atataattat agaaaataaa aagtcagtaa
      361 ttaaaaaaca agattgtcta atgtgtggac attgtgcagc aatatgtccc acaaaagcta
      421 ttaccttgac aggatttgat gaacctccta tagaacttac aaataagcca aagctagatt
      481 cagatgaatt actaatggca attaaatcta gaagaagtat acgtaaattt aaagacaaag
      541 aagtttcttc agaaattata aagcaaatta ttgaggcagg taggtatacc cctagtgcta
      601 aaaattcaca agatgtatca tatattgttt tagacaataa aaagtcaatt tatgaaaacg
      661 aagcagtgaa attttttaga aaaataaaac ctattgcaaa tatagctatt aaatattcaa
      721 aagaagttga gattgacgat aactttttct ttaagcatgc tcctattgca ataatgataa
      781 ttacaaaaga taagattagt ggttcacttg cagcatcaaa tatggcatta atggcagagt
      841 cttatggcct aggtgtttta tttagtggat atttttcaga tgtagcaaat aattcgccaa
      901 aattaaaaaa gcttttaggt ctaaaacgca gtaatcatgt tcttacaaca ttagtaattg
      961 gatacccaga tgttaaatat agacgtactg cacagaaaga agtagcaact gttagatact
     1021 tataagagag ggtggatttt atggaaaaag aatgtcaaca aagaatgcaa gaaataacgt
     1081 ctaaatttaa agaatgtcgc aaagctatct ctgctatggg tgatgagact agacaattga
     1141 ttttaattgc tctcttagag agtgatttta atggtatacg agtgggagaa atcacaaaaa
     1201 aaacacatct ttctcgtcct gctgtatctc atcatcttaa gatattaaaa gaagcagaga
     1261 ttgttaattt gcgtagagaa ggaacaaaaa attattatta tcttgattca aaaaaatcac
     1321 aatggaggtc actaacagag ctaataaatc ttatatttgt tggaatacaa catattagtg
     1381 aaaatgatat aagaaaaggt gattaaatta tacttgattt aatacaaaaa gattgattta
     1441 ggaactaaaa agaactaaaa agttcacttt gagacactat cttttgaact atacccatct
     1501 taggagacag ttaaaatatg taaaactgta taaaacaatg ggtatagttc attttataat
     1561 ggtttaaata ttaagaatat attattctaa atagtatctt tctctgtttt gagttttatt
     1621 tttatactca atagcttcag tggggcaacc acaaatacat gccatacaat gagtacagtt
     1681 atttttccaa actggttttt tgttttttaa attaatatta tttaaaggac atagctctac
     1741 acattttcca cagccaatgc atttatcagt ataatgaaat cctttggcac taataattgt
     1801 cttataaaaa actgtattta ttataccact tttaattgta cctcctaagc cactaggagt
     1861 aataatttca aattcatttt catttcgtat atcagaaata atcctatata tttgtttatc
     1921 tgcttcttta atcatttgtt ttgatgtttc tttgtctgtt gtagaaaata aagctatata
     1981 attctctggc attttaattt ctgccatacc ttttaattgc cattccttgt atttacatag
     2041 tttttgaata tagtttatgg attttgctgt atctccacca caagtcatta caaagtaaac
     2101 tctgttgctt cctagaaatt ttgtttcttt tataaaatcc gtaaccacta ttggcaatct
     2161 ccatgcataa gtaggacata caaatatgaa tggtttatct gttgatatta attcatctgt
     2221 tttttcctct tttataagtt gatttagtga tatcagttca tcatttaatt cttgtgcaat
     2281 tttacgagcc acatatcggc tattaccagt tccgctaaaa tataaaatca taataatatc
     2341 tccctaacta ttttttattt taactcttaa ttttttattt attatagtaa tttattgata
     2401 tattctattc aaatgtatgt aaaatttagt agttaagaaa attataacac attagtaatt
     2461 tttatactta ttggtaaaga atttgattac cattaaattt agcaacaaga catcaactgg
     2521 acttgtatat gagaagggat aataatttgc ttatgaaaga ttataaatat aaaggttatt
     2581 ttgaaggtgt agaaatattt gataatgtaa atgaccttaa cttaaatact ttaacaacag
     2641 ttagtagatt tgtgtttgtt tttttgttta cgataactta aggaaatatg ttacctgaac
     2701 aataaattta tataataaaa gtatataaat atatatatga atgttaagat tagttgatag
     2761 aatttaaata ataaatccaa tattataaac ataggaggta tgaaaaatat gattaatgag
     2821 aatttaaaaa gtctaagaaa aatcaatcag tatacacaag aagaattagc tgaaaaactt
     2881 aatgtttcta gacaatcaat agcaaaatgg gaaagtggag aatctattcc tgatataggt
     2941 agttgcatta aacttgcaaa attgtacaat gttaaactag atgacttagt taatcattcg
     3001 gaagaaaaaa caggtattat agtacctcct aaaggaaagt ttttctttgg agcagttgta
     3061 gttggtgaac gtggtcagat tgtcattcca aaagaagcaa gagaagtatt taatataaat
     3121 gcaggagata aattattggt acttggagat gaggaaagag gattaggaat tgttcatcaa
     3181 cgtgatttga taaattttat aggtcatgtg ggagtagtac ataaagaaga atgatttaaa
     3241 aaaattataa aaggaggtat gaaatatgag taaaatagta ttttttagca ttcctgcata
     3301 tggtcatact aatcctacta ttgaagtagt tcgtgaatta gtggatagag gaaatgaggt
     3361 attgtattat tcttttaatg aatttaaaga taaaattgaa ggtgctggag caaaatttat
     3421 ttgttgtgat aaataccttc cagaattact ccctggagat gagaaaaaaa ttggtaagga
     3481 ttttcctgga ttaattgaga tgatagtaga tactactatt tctttagatg aaaaagtttg
     3541 tagagaactt aaagatttta atccagattg cattgtatca gattcattaa gtttttgggg
     3601 aaagttattt gcacaaaaac ttaatattcc atatgtatgc tctacaacaa cttttgcatt
     3661 taataaacat acagcaaagt tgatgaaaca aaactttata gaaattatac gtatgatttt
     3721 tggtgtaaga cgcattaata aaaaaataaa gcttttacaa agaaaagggt atgaagtaaa
     3781 aaattttatt tctatagttt ctaatgataa tgatacggat accattgtat atacatcaaa
     3841 agaatttcaa ccaatggtag aaaccttctc tagtaagtat tcatttgtag gtccatcagt
     3901 atcaaagttg attattgaac ctaaagagag aaaaagaaag ctaatatata tttcgcttgg
     3961 tacaattaat aataaaaatg ttagctttta taaaaactgt ataaatgcat ttaaagattc
     4021 tgatgttgat gtgattatgt ctgttggtag aagtactgat attaaaagtt taggaaatat
     4081 accaaataat tttgaggtta aaaatagtgt tgaacagatt gctatacttc aaaaaactga
     4141 tgtgtttgtt actcattctg ggatgaatag tgttaatgaa agtttatatt atggagtacc
     4201 tatggtatta tttcctcaac attcggagca gagaatggta gctaagagag ttgttcattt
     4261 gggagcagga attatgttaa aagaggataa atcagaaagt attaaaaaag cagtatttca
     4321 agttataaat gacaatgaat ataaagaaaa tgcaactaaa ctatcaaaaa gcttttataa
     4381 tgcaggaggc tcaaaaaaag cagcagatgt aatattacag attatatgta atagtagaca
     4441 agttgacata taatagattt gttaaaaata tatatacata catgtaattt aatcaaagta
     4501 ctgacagtgg ggtcttttaa cctaccagtt ggggcatata tattgatgct aaatatgagg
     4561 gatgttatat tttaataaaa aagataacat ccttttttta gtgcattata tcgagtttta
     4621 aataataaat gaaggaggat taatatgaaa aaatataaag ttatttttat agtattaatt
     4681 ataggtatta taagtgtaac agggtttgtt gtatataaaa actgcattaa accatggaaa
     4741 gatgctgaaa catatattga taagtatatg gtaaaacaag atatttcaaa agataatata
     4801 aaaagtatta caaaagaaaa agccaagaag gcaagttacg aaggaatact atacaaagtg
     4861 tattacaaag atgaccctcg atatgagtat caatattttt atagtgataa ttattatgct
     4921 ctatatgtaa ataaagtaat gttacaaatt tatgatatgg aaaataataa taaactttta
     4981 agaagtaatg aggaattaaa aagtgtaaag tatccaccaa tatatcttaa atataagcca
     5041 gataaatgat ttatgacaaa atagaataat ttttattaaa atttatttaa aaatatcact
     5101 agagcgttta aat
//
LOCUS       DAELTA010000212         5047 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.216, whole genome shotgun sequence.
ACCESSION   DAELTA010000212 DAELTA010000000
VERSION     DAELTA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.216"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            672..4814
                     /locus_tag="KPG13_003360"
     CDS             672..4814
                     /locus_tag="KPG13_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG0987047.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         1956 a          730 c          838 g         1523 t
ORIGIN      
        1 aaattcagaa aaaaatttta gtttacataa taaaaataaa gtcaactaaa attattgatt
       61 ttagattaaa gtaccggatt aagcatttaa gtagatttta agcttgattt gattagaaag
      121 gtacaaacta tcaccgaaaa aaattaaacc catttaaaaa gcaatataaa agcttgttat
      181 ttttgagaaa aatagaccct ctaaactcaa ttagattaaa aataaaatac agaaaattat
      241 atctctaaat atcttaaata attaagttta aattttacga tattttgttt gatgatttat
      301 aatataaaag catactctta atcttcaaaa tttaaaaaga gtaaaaaata acaaaaaaat
      361 cactgacatt tcactgacaa attgtaattt tgttattata aatacattga aaatacttga
      421 ttattgaaaa atggcataaa ataatacatt ttatgccatt tgctattatt tgatagtttt
      481 taacttatga cataagatat tttttaacat atacaataaa atataaaaaa attgatattg
      541 caaataaaaa aaaatacata ttagatgtta tagaaaaaaa gcgcacttat aggcttaaaa
      601 tgtcacataa aagagtttta aagaataata tattatgaac acaaattttg taatcacaaa
      661 ggagtgataa tatggcaaca gtatcaggac aaatagtttt tgatagaaat agaagtgcta
      721 ccattgattc tggagactca ggaatagcca atataccagt tgtattacaa gatatagcaa
      781 ctggagttag acttgtggtt ctcacagatg catcaggaaa ttatgttttt actaatgttc
      841 caaatggtaa ctatagaatt gtagaagctt ttggaactac aggaggagta ccaacaccag
      901 gaaattttgc aacagcgata gtaggtccag ttccaattgc agaaatgcca cctattagtt
      961 ctgtgacaag tcctcctatt ggttcaacaa acttagatgg tacaacacct aacacactat
     1021 taataacagt agcaggttca gatataacaa atcaaaatat attaaatggg cctgtgattt
     1081 atacacctat agagaatata ttggattctt gcgtatctgt atctaatata aaccttatta
     1141 cagatgcaga taacggaaca ttcggttttt ttccaccagg tacaccaagt aatacaggac
     1201 ctgaaataga accttaccca aatgtgacgc cagattttac gtatgttgta ccagacccaa
     1261 ctaagtttac acctttagat ggagaatata cagtacaaaa tattatgaca aatgcaatga
     1321 gtaatgttat aggagcttgg tggcgtattg ctgaccatac tacaggaaat gaaacaggaa
     1381 gaatgatggt ggtaaatggt tttaatcctg gagctgtatt ttttaaatca acagtacaag
     1441 ttacacctaa cactaattat ttatttagca cttggatttt aaaccttttt aaggtaacag
     1501 gatatccacc accacagcta ggtgtaagaa tatttgacca aaataataat atactatatc
     1561 aagcaacatt aggagctcaa attccagtta atacaaacgc accagaatgg aaagaagtag
     1621 gtactgtgat taattctcaa aataataatc aacttacagt agaatttttt agtgaaggag
     1681 aaagcgccat tggaaatgac tatgctatag atgatgtttc tttaagagaa atagctgttc
     1741 caacttttac tccaataaaa acaagtaaca cagaaactgc taatgtaggt gatataatca
     1801 catatacagt tacattagat aatacttgtg atagcccttt aactaatgta ttttttaggg
     1861 atttaattcc aaatggttta gagtttatac ctggaactgt aacggttgat ggagtatctg
     1921 taccaggagt agaccccaat acaggtttcc cacttccaga tattggagga ggaacagggg
     1981 ttgaagtaac ttttgatgtt gtggttgaaa gtattcctaa cccaaatcct actaataata
     2041 tagctactat agattactcc tatacacctg tagaaggcgg tataccaaat gatttttcag
     2101 tagattctaa tccagttcct gttgaagtaa tctcagcaga tattgaagtt acaaaattat
     2161 ctgaacctac tatagtgaat ccaggagagg aattaatata cactataaaa gtagtaaata
     2221 atggaccatt cccatctgaa aatgtggttt taacagatga tgtaccagct tctattgtaa
     2281 atccagagta ttcattagat ggaggagtaa cttttcaacc atggactgga agtttaaata
     2341 tagggacatt agaagtagga gagacaagag ttattataat taggggtatt gtaaatccat
     2401 caacagtagg aattataaca aatacagctg tagtatcttc aactacagca gacccaaacc
     2461 ttaataataa tacatctaca atagagacag aagtgaattt attagcagat attttagtaa
     2521 tgaaaacagc agaacctaat tcagctgttc caggtacttt attgagatat actattcaag
     2581 tagaaaactt aggaccagca aatgctgaaa atgtaatatt aaatgatgat attccagctt
     2641 ctattataaa tccagaatat tcattggatg gaggagcaag ttttcaacca tggaatggaa
     2701 gtcttaatat aggaacttta aactctggaa tttcattaac agttttaatt caaggaacag
     2761 ttagtttaaa ttcttcagaa tatatagtaa atacagcaac agtttcttca actacacctg
     2821 acccagattt aagcaacaat atatctacca ttattacacc agttaaccca caagcaggta
     2881 tttctattat aaaagtagct gacgaggatg tagctgttcc aggagaagaa tttgtatata
     2941 caattgaaat ttttaatgaa ggaccatcta atgctacgaa tgtagtttta acagatgata
     3001 taccagatgt tattttaaat ccagaatatt cattggatgg aggagcaact ttccaaccat
     3061 ggactggaag tcttaatata ggtacagttg caccagggca attaataaga attatcataa
     3121 agggattagt aagttcaact gctacaggag atattactaa tacagcagaa gtaagtgcag
     3181 atgtaccaga accagttacg gattcatcaa cagtgacaac tcctatagtt ccatcagcag
     3241 atatagaagt aataaaaact agcaatatgg atacagcagt tccaggagaa actttcagtt
     3301 atactataac agttacaaat ttaggaccat ctgctgcaca aagtatcgtt ttgacagatg
     3361 atataccaga tgttatttta aatccagagt attcattgga tggaggagta actttccaac
     3421 catggaatgg aaatcttagt ataggcactt tggatgctgg agaaataaga agtattataa
     3481 taagaggaac agtgagccag acagcagtag gtacaattat aaatacagca acaataagtt
     3541 caactacacc tgacccaaat ccagataata atacttcaac agatgaaaca gatataagtc
     3601 cattagcaga tatttctgtg ataaaaggca acgaaccagt tgctatacca gggggaagat
     3661 ttatttatgg aattgaaatt gcaaatgcag gaccatcttt tgcagagaac gtaactttaa
     3721 cagataatat accagcttct attttaaatc cagagtactc aatagataat ggagtgactt
     3781 tccaaccatg gaatggaagc cttaatatag gtactttaga tgctggagag ataagaaata
     3841 tcataataag gggaacagta agtcaaacag caataggcac aattataaat acagcaacag
     3901 taagttcaac tacacctgac ccaaatctaa ataacaatat ttcaacatca gaagcagagg
     3961 taagttcatc agcagatatt tctgtagtaa aacgaagcaa tcagactgtt gttgtaccag
     4021 gagatgtact agactataca attgaggtga gaaatgcagg accgtctact gctcagaacg
     4081 taactttaac agataatata ccagcttcta ttttaagtcc agaatattca atagataatg
     4141 gagtgacttt tcaaccatgg aatggaagtc ttagtatagg cactttagat gctggagaga
     4201 taagaaatat tataataagg ggaatagtaa gtcaaacagc aataggtaca attataaata
     4261 cagcaacagt aagttcaact acacctgacc caaatccaga taataatact tcaacatcag
     4321 aaatagatat aagttcatca gcagatattt ctgtaataaa attagcaaat aaaacagaag
     4381 catgtgtagg agagcaaata gattttgtaa tagtagtatc aaatgcagga ccagaaaatg
     4441 ctcaaaatgt tactttaata gacaatgttt cagataagct taagaaagca gtattttcac
     4501 ttgatagagg tgtaagtttc caaccatgga ctggaagtct taatataggt acactaccag
     4561 ctggaacttt aagagtgatt atattaagag gaattataaa atcaacatgt cttgatagat
     4621 taacaaatat tgcagaaatt acttcaacaa caccagattc aaaccttaac aataatatat
     4681 caagagtgca agtagaaata aaacaatgtt gttgtaatga ttgctgttgt aacaactgta
     4741 gtaattgttg ttgcaatgat tgtggttgta aagattgttg ctgtaattgt tgtaaaaatg
     4801 acttttggtg ttgattttat aaaataattt tgtatacctt aaattaaact gaaataaaaa
     4861 ataaattcac atatgagtaa aatattttat aaatattaat aaattttcta tctttgatat
     4921 ggaccacatt cgtaatacta gttattaaat gaagtggtcc tttttaattc attaaaatta
     4981 tttattctaa ttaaatttta aatgtattaa ataattttaa ttttttataa aaaatattca
     5041 tatgtta
//
LOCUS       DAELTA010000213         5009 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.217, whole genome shotgun sequence.
ACCESSION   DAELTA010000213 DAELTA010000000
VERSION     DAELTA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.217"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..332
                     /locus_tag="KPG13_003361"
     CDS             <1..332
                     /locus_tag="KPG13_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG0987048.1"
                     /translation="VFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNF
                     DPSEIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYE
                     EEITNME"
     gene            complement(537..860)
                     /locus_tag="KPG13_003362"
     CDS             complement(537..860)
                     /locus_tag="KPG13_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG0987049.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(853..1554)
                     /locus_tag="KPG13_003363"
     CDS             complement(853..1554)
                     /locus_tag="KPG13_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG0987050.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(1832..3217)
                     /locus_tag="KPG13_003364"
     CDS             complement(1832..3217)
                     /locus_tag="KPG13_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG0987051.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(3325..4563)
                     /locus_tag="KPG13_003365"
     CDS             complement(3325..4563)
                     /locus_tag="KPG13_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG0987052.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(4576..4944)
                     /locus_tag="KPG13_003366"
     CDS             complement(4576..4944)
                     /locus_tag="KPG13_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0987053.1"
                     /translation="MKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT         1804 a          672 c          604 g         1929 t
ORIGIN      
        1 gagtatttga aaatctttta tctaatatag caaaatattc cttagaagaa actagagtct
       61 atattgatgt atatgactat ggtgaaaatg taaaggtaac tatgaaaaat atatcttctt
      121 atgaattaaa ttttgaccca agtgagatta tggaaagatt taaaagagct gatgaatcaa
      181 ggaatactga aggaagtgga ttagggcttg ctattgcaag agatttagta gctttacaag
      241 gtggaaaatt ttatatagaa atagatggag atttatttaa agcaaattta gagtttcaaa
      301 aatatgaaga agaaataaca aatatggaat gagatagtat ttaagtatat atattttttt
      361 actgtcgaaa gcttttatta taatatatta gaatatttac agtagagaat gaagagtaaa
      421 aacagagtaa ctttaagtca ctctgttttt tatttaaaat caataatata acaacttaaa
      481 ttaaaattgt acacatagtt ttaaaataac ttatacaata aaacattatc caaatatcac
      541 aagataattc caaataaata tatagatatc actcctgtaa taatagaaat accaatagat
      601 ttaaacttat atgcaacaag aagtacaatc ataaaagata taattttagg gttagctata
      661 gataaatata gactatctcc attaaaaaat acatctttag cagttaagga agtaagtatt
      721 gctacaggta taaatttcat gatttttttc aaaaaatcag gcatctcttt ttttgaaaaa
      781 attaacatag gaagcactct tggtagatat gtaacaatgt acattccaat tataataaaa
      841 aatatataat tattattcat aagattcctc ctttttacag taagattctt ctataaagaa
      901 accaattaaa gatgctaaaa ttgatgcaac tattattact aaattgcttt tgaataatgc
      961 atataaaata acagaaataa tgattgatga tataaatact acaatataaa tcttattttt
     1021 tatttgagaa actattaaag ctataaacat agaagtcaat atgaagccag aaatagaact
     1081 gtctatagat atactttctc ctaaaaatgc tccaataaaa ttagctaaaa ctgatgaaat
     1141 taagcagaag aaattaagat aaatagcatt tctatcagac caatttccag cagtaaattt
     1201 ttcataatta actgcaaaag tttcatctgt tatagtatga caaaatggca atataaattt
     1261 tggactttta ttttttatgt acatgttcaa attagaactc attagaaaat gtcttaagtt
     1321 aactataaat gtagtcaaaa caatagatgt tacacttgca ccagtagaaa tcattgaagc
     1381 tgcaatgaat tgagaacttc ctccaaatac taaagttacc attagtgcta tttgaaaagg
     1441 tgaaagacca gatttttgta atagtattcc tcctactata cctataggta tataacctaa
     1501 gaatatagga attgaagcaa taaagctttc ttttaattgt tgagttcttt ccacaataaa
     1561 tcactccttt tgtattaaat gtattaaagt attcgtaaaa cagaaaaata gattaattgt
     1621 ataattgcaa ttataattaa aaataaattt ggattaatat ataaaatact atacaacaaa
     1681 tagataatct taaacggtta ttaaatttta tctatttgtt attatgatgt aataatacat
     1741 attaatctct ataagttaat tactatctgg tgtaatttta ttttaacaac aaaaaaaaat
     1801 acacacaatt aaaatattgt gtgcaataca attatttttt ctcttctaat agtcttttta
     1861 atacgctcaa accagcttta agttcttctg tactttctgt agcacataca gctattctta
     1921 ttgcagaaat tggtttttct tttccaacag caaaacgttc agaagcatag acctgtacac
     1981 cttctttaag tgcttgtatc tcaaattttt ctgccattat tccatcaggt agaatcaacc
     2041 atctaaatat acattcttca tttcccaaaa gattatatcc agataaatat tgattaataa
     2101 tcttgtttct tcttcttgca gatttacggc gtgcattaat taatttatcg gcttcacctg
     2161 atgtaatcat gcgataagca atttctgtta aaaaataaga ttgagataga ttaatgttgt
     2221 ataatgcgct tgataatgcc tctcgatact gtttaggtgt agagatatat gctagccgta
     2281 aactaggtgc aataattttt gataaactcg taatataaat tgtttgattg ggcagatagg
     2341 aagccactgg atttaaatgt gtttcattaa gtaaactatg aatagcatcc tcaataacaa
     2401 ttaaattgta tttagatgca atatttgcaa tcatttttcg tccattttgt gacattatat
     2461 gtgtagttgg attttgataa tctggtatta tgtatagacc tttaatattt tcatttttac
     2521 aagcatataa tagtccttct tcactaatct cattatcttc ttgtttaatt ggaattagtt
     2581 gaatcccaag cattttagct gctgtcttta ttccaggata agttaatggg tctacaccta
     2641 ttctatctcc atgttgaaac aaaccagcta aaatagcaac aattgcattt tgtccaccac
     2701 ttgcaggaag taagctatct gatgtagttt caagcccagc tttataaatt aatttggcaa
     2761 tagcttcttt ttgccattta gcaccatctc tttgtccata ttggaatatg gttttaaaat
     2821 ctatttcctt aattatgtgt ttaaacaaag tattcatttc ttctagtgtg aaatctggat
     2881 tcattgtgcc catttcaata aatgtaattt tattattact ggacatcaag aatagatttg
     2941 aacgtgtatc ataagataca aaagttccac ttcctgtaac tccacttatt agaccttttt
     3001 tttcacaaat tttaaaagct ctagagatag tactcacatt aacatctaag aaatctgcca
     3061 attcacgttg gggtggtaat ttagtacctg gtaataaaaa tccattgttg atatcttgtt
     3121 ctagttgttc tgcaagcgcc ttatataata tttgcccttt tgaaggtctc tttggtttcc
     3181 agttcatagg ataattttca aaagaattaa ttggcataag ttttcacctc acattattaa
     3241 tttgattttt tctttatcta aaacaataaa ttctaaagat acaaataaaa actgatagga
     3301 aataattcta tcagtttttg tcttttaatt gatttcaaat cttcctataa tttttatacc
     3361 ttctttatca taaagaggga ttgttttttt aaatatatta aaatttcttt ttcttgtata
     3421 taaaacagct tcttctggtg tctttggttg attatcacaa tcgtacaaat ctcttttttt
     3481 tacgtagccg acttttttac ctctacctat tgtttgtata agttctggtt caatggaatt
     3541 tccatttgag tctattatat gtgtaccata ggtttctcca tttttattat aattaatctt
     3601 accaatagta tattctacat cttcattttg tttaaaattg gagttgatag aaaatattga
     3661 ccctattatt ataaaggtta ataaaaactt aagacctaaa ttaattttct ttttatcttg
     3721 attaaacata gaatctaacc tcttttttaa aataatattt tttttattta gaaactgaga
     3781 tacgaatgat gaatacttca aagatttttg atactttatt gtatctaata ttatatatgc
     3841 atactcttta atttcactcg tggtacactt gtcaatcaca atttcatcac aagataattc
     3901 acataattca tgaaaatatt tttttaataa gtataaaatt ggattgaacc aatatatgat
     3961 acaaattata ctaattagaa gcttaaataa gttatctttt cttttataat gaattaattc
     4021 atgtttaaag atgaatctta actctttatt attgtacttt ttttctggaa ttattatttt
     4081 acagcgtgtt aaacctataa tggcaggaga ataaatacct ttaaccttat atatctgaag
     4141 ttttttattt atacttagtt cttttgcttg ttcatctaag atactagtaa tatctatttc
     4201 taaaatttct tttttaaaat tttctatgta attataaaat ttaaaatttg ttcttaaata
     4261 gtaaaaagag attgctataa ttcctaatat ccagatgtac ataaatatat ctaaagtaat
     4321 gccctggtca tatggtatat aattatcgat attatgctta tagatattaa tagtagtatt
     4381 attgatattt aatatattta aatttatttt agatataaac atcattctga ctactattaa
     4441 aagacatata aaataattaa acttaaaagt atatttgctt atctttttag taaataaaat
     4501 gaaaaaaaga attagtggac ttatgtaaat attaaattta ataacttcta taaataattc
     4561 cataaaatac gtctcctatt ttcttttttt tagccattgt tcaagttctt ttatgtcatc
     4621 atctgatatt tcatttccac aacctaatga tgatataaag ctatgaatag aatttttatg
     4681 tataagctct aaaaaatcct ttgtttcaaa ttttagatat tcctcatgtt taactttaat
     4741 agtgtataaa gtatttctac catctttttc aaattctaaa aaacctttat cacttaatct
     4801 agaaagtact ttaccagtag taccttttgt ccatccatga gaaatttcca tataattagc
     4861 tacttctctg gctggtattt tatctatgtc tttatcccat atgaaattca taactaacaa
     4921 ctcggatttt ggtatttttt tcaatataat cactcacctt taacttgatt atattagaca
     4981 aatgtctaat agtcaatata atatatcat
//
LOCUS       DAELTA010000214         4951 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.218, whole genome shotgun sequence.
ACCESSION   DAELTA010000214 DAELTA010000000
VERSION     DAELTA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.218"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..633
                     /locus_tag="KPG13_003367"
     CDS             <1..633
                     /locus_tag="KPG13_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987054.1"
                     /translation="DSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(761..1951)
                     /locus_tag="KPG13_003368"
     CDS             complement(761..1951)
                     /locus_tag="KPG13_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0987055.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2057..3436)
                     /locus_tag="KPG13_003369"
     CDS             complement(2057..3436)
                     /locus_tag="KPG13_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG0987056.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(3467..4675)
                     /locus_tag="KPG13_003370"
     CDS             complement(3467..4675)
                     /locus_tag="KPG13_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987057.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         1634 a          951 c          586 g         1780 t
ORIGIN      
        1 gactctactc tagaccaagt ttataaaata ttaaatagta ttaaaaaaga taaattaacc
       61 gattttataa ttgaaaaaaa tagtaattct tttaaaattt tcactaaatg tgaaacgatt
      121 aaatcattaa ctaaaaattt tactgcatgt tatataaaag aatctattga aaatagtaca
      181 aatattaaag tatgtattgg atatggaatt ggtaatgata taaagcaagc tatgtctagt
      241 gccataagtg ctaataagga atctaaaata aatcctttaa attacagttt cttagttaac
      301 gaaaattatg agacaatagg acctttactg tgtgataagt tacttactgt ttcaaataaa
      361 gtaactccac atataaatac aattgccaaa agttctaaat tgtctactct tactattcaa
      421 aagattatat ctgttactga aattctacaa agagatgaag ttactcctaa ggaattatct
      481 tcatgtttaa atgtaacagt tagaactgct aatagatttc taagtagtct cttaaagtct
      541 ggaaacgcta ctatattata tgaaaaacaa aactcaacaa aaggcagacc tgaaagggta
      601 tacaaattat ttatatcaga aaataaatat taaaatccaa aatgcataaa aatattttat
      661 catctaatat taagtaaaat tatatatgac tttctaaaag gtatatcttt gcatcttttt
      721 aagatatacc ttttatgata tttaataata ttttatgtta ttatctacta aaattatttt
      781 ctaaccattt tactgcacaa tgagcaaata aagctgaacc ataaactaat ccctcttcat
      841 taaaaattgc tttaggatta tgcataccaa aattatatcc ctgactaata cttcccattc
      901 caatccataa aaatactcct ggaaccctta gtaaaacctc tgaaaaatct tctgaaccca
      961 ttgcagatgg aaagtctatt acattttcat ttcctataac ttctttaaca taagatacaa
     1021 tctcatctcc tacatccaaa tcatttataa ctgcaggagt ccccatttta aactccactt
     1081 tagcatttcc tccaaatgat ttagctattc ctgtagatat ttcaataatt ctatttttta
     1141 atttttctct aacaaattgg tcaaatgttc ttaaagttcc tgacatagat gctttatttg
     1201 gtatagcatt tggtacgttt cctgcattaa acataccaat agttaaaact acaggctctt
     1261 tctgaggttt ttctcttgaa agtatagtgt ttaatgctat ttgtatatgc gacattatgt
     1321 ttattgggtc cactccaaat tcagggcttg ctccatgagt tgcttttcct tctactgtta
     1381 tttcaaatat atccgcagat gccatagctg gaccacgtga ataagctact tgacctgtgt
     1441 taagaaattt acccaccata ctgtgaaatg ccatagctgc atcgacctga ggattttcta
     1501 atataccatc atcaatcatt gattttgcac ctctggtagt ctcttcatct ggttcaaata
     1561 caaactttac tctaccttta agtaagtgct tattatccat aagtatttta gctgctccta
     1621 agagcattgc tgtgtgatta tcgtgtccac aactatgaga tgcatcttta ttcttacttt
     1681 taaaaggtaa atccgtctcc tccctaatag gaagtgcatc catatctgcc cttaataata
     1741 aggtctttcc accattttca ctgtctaaaa cagctactat tccattatta gacgtcaatt
     1801 tcggagaata tcccattttt tctaaagtac tggtaacaaa ggttactgtt ttcggtagat
     1861 tgcaaccaat ttcgggattt tcatgtaaat accttctcca tctaataatt tccccttcta
     1921 ttgactgcgc ttctttaaaa aaatttttca tattccttcc tcctaatcag atagtcagca
     1981 aaatctcact ttcttttagt aatgaaatga tgctgatata aaaattataa ttttatatat
     2041 taaatttcat gtctttttaa ggagttggaa tatcatctgg aataggacat ttatatgttt
     2101 taccattcat agatttatca aattcttctt ttgccttttt agctatctct ggattattta
     2161 atattttagc tccaaacata gccataatct ttgatgcata tatcatacct ttttctccta
     2221 tagaacttcc tgcacaagat gtaaactgcc agctatgacc tggtgctcct agattcgtac
     2281 accctgtaaa aaagtatgct gtcggaacta tatgcattac atctccaata tcagtagagc
     2341 cataactatt aaagtttgta acatttcctc caccataata tagatgagtt ccttctggca
     2401 aattatattt acttatcata gccttatatt gttgtgggct ttgtctatct aattcagctg
     2461 caaaatccaa ttcctctcga gtccaaggct cttgtttaat ctcattcata cattctgata
     2521 caagatttgc taaaacatga ttgtttaaag tattataaca ccctcctaaa aactctactt
     2581 caacttcagt ttcagtcatc attgcagctc cttttgctac ctttacaagt ctttcatatg
     2641 tactttctac agcttctcta cttaaagctc ttgtataata ccatactgat gctttatcag
     2701 gaactatgtt gggtgctact cctccatctg taattgtata atgaatcctt acatcagaag
     2761 taacatgttc tctcaaataa ttagctccca cattagtaag ttccacagca tctagtgcac
     2821 ttcttccatt ttgagggtct gaccctgcat gtgcagtctt tcccttaaag tgaaacttta
     2881 cagagttaac agctgttgaa gtaccaattg tagcttcact aattttattt ggatgaaaat
     2941 ttatagctaa gtctaaccct tcaaacgctc cacctctagc cataaatcct ttacctgtta
     3001 attgttcttc tcctggacat gcataaaata ctatagtccc acttaagtta ttttctatca
     3061 tctctctttt aagccctact actgccccta catgtgctgt acataataaa ttatgcccac
     3121 atccatgacc atatgcaccc aattcaaatg cttcttgctt tgttgaaact ttttgattta
     3181 aaccaggtaa tgcatcaaat tctcccataa aacctataac tggtttacct gaaccaaaac
     3241 tagctctaat agccgtggct aaacctccta cacctacttc cacatcaaat ccttcacttc
     3301 ttagaaagtt tgctgtattt tcacatgctt taaattcttt aaatgctttt tctggatttt
     3361 cccatatttt cttacttaaa tcatcaagtt tgaatctttt tttatctatc tcatttatag
     3421 atatacttcc tatcatttcc tattcctcct ataagtatat ttactatcaa ccaaatataa
     3481 ttggacctat tatactagca aatgccactg atgcaatggt tacagtaaca aatcctccta
     3541 taatcatctt tggtaataaa taatctgttg ccctttgttt ttcttcttca gttccttcca
     3601 tagcatcaat accttcgttg gaaattatct gtgttgcagg ataagctagc atacaagtta
     3661 caccaattgc agctgacatt cttggagaat atcccaatag ttttcctaca attgcagtaa
     3721 ataatattat aaatacagaa cctacaacaa gtattcctat agttggaaca atcatcccaa
     3781 acaatgctgt tggagttacc ttagcaagtc ctccaggtaa catcaacata caaccaaaca
     3841 ttataattcc attagattga gatttatcta aaattccttt ttctaaaaaa cctattcgtg
     3901 taaaaatcaa ccctactaac aaacatgcta tattaggatt taaataatag tttgcagggg
     3961 taaatcctgg tattaaagta gccaatccta caaaattacc aatcatagca acaaatgaaa
     4021 gctttgcaag atacactaga gtagatgatt ttttacctaa atcttcttta aaaattctca
     4081 tacttggaag tttaaaatta ctttttgact catctttcaa aaaatccccg ttatctcttt
     4141 taaattttaa ttctttattt atacaatatg tagttatcgg aataccaaag aacttttgaa
     4201 atgataatac tagaattaca aatgcagcaa gttcaggtct atttgcagct tctgcaacag
     4261 ttgttacgat aatcccagca acagtagaac ctgctattgg aggtgctgct attaaagcat
     4321 actctctgcc aaataacata ctacccactg ttaaacagcc aactcctatt cctacaatac
     4381 ctactaatgc aattacaacc gttttccatt catgacataa gtcttcaaga tttataagtg
     4441 ttcctaagtt agttattaat aaagctatac caaagttgct cattgcagct aatatccctg
     4501 atgctcctgt aatgtctttt ggtaaaatat ttgtccaaaa tcctagtaaa aatataatac
     4561 atacaaataa caatgttgaa attgcacctt ttgttttctt agataaaaac tcactaaaag
     4621 cataaatcat tacacataca gtaaagcaag taagtggatt ccaaaaattt tccattgttg
     4681 tttcctccta aatataataa ttttttatca ataaaacaat aattataaaa tgtatattat
     4741 gcagttattc ttaactacat atggtatagc ttgatatttt ttgatgatat tatatacgtc
     4801 ctaaattatt cctaaaatat ctaaataaat ataactagag ttaactctct aaatattaac
     4861 tatagttatc aacttactag atttcttttt gatttttact ttctacataa ttttacgagt
     4921 tagaattgta cttcaactac tctctatatt a
//
LOCUS       DAELTA010000215         4923 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.219, whole genome shotgun sequence.
ACCESSION   DAELTA010000215 DAELTA010000000
VERSION     DAELTA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.219"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(554..1195)
                     /locus_tag="KPG13_003371"
     CDS             complement(554..1195)
                     /locus_tag="KPG13_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG0987058.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1438..2241)
                     /locus_tag="KPG13_003372"
     CDS             complement(1438..2241)
                     /locus_tag="KPG13_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG0987059.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2397..3572)
                     /gene="thiI"
                     /locus_tag="KPG13_003373"
     CDS             complement(2397..3572)
                     /gene="thiI"
                     /locus_tag="KPG13_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG0987060.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3618..4769)
                     /locus_tag="KPG13_003374"
     CDS             complement(3618..4769)
                     /locus_tag="KPG13_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG0987061.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1526 a          931 c          472 g         1994 t
ORIGIN      
        1 ctatttattg aaaatttaga tatttttcta aaaattatct atgttaatag ttaaatttat
       61 gaacattttt tgtttttatg ttaggatatt tcttagaaat gaaatacttc ttaatttgat
      121 ttgatatgta tatttttata gctattttat tataaaaaaa aagaaccctt tttataacta
      181 agggttactt tttttctcta ttttttctta ttatatcact attttttccc tatttcaata
      241 ttttgacatt ttttccatta tactgaaaag atatcttaaa tttttaactt ggataaattt
      301 taaagcatat acttaaaatt aacatacttg ttttctattt tattacactt aatccttatt
      361 tttatcattc tacaaaacaa aaataaaaaa gaataagata ataaataaaa tacttatcac
      421 cttattcttt atttaattat tttacaacga aaaatctaaa ctacttttta aatagcttcc
      481 tcttccatca atttatttgt atattactga tttaattctt caagtaactg attggttctt
      541 gcaatacttt atcctaaact ctggaaactt tgtaaactac ctctctagca tttggtaaat
      601 ttccatctgg aatacctaca gcaataccaa atggtacaga atacccttct ggtatatcaa
      661 gtagttctct gtatttttta tttgtatcaa acttcatctt tattgactct gattctttta
      721 catcttcttt atccaataca ctcacaagac cttcaatatc cccttgttca acaggtgtaa
      781 ataaacctct aaccattcct attatacaac ttccaagccc tagtgcttgt gcagctgttg
      841 ttatattccc tgtaagtatt cctatatcat atggagccca tgaactactt tcatcataag
      901 aaataaataa aacggttgga gcaccataaa ataacttaaa gtttttattt atctcaacat
      961 tactcacctc atgaatccta gataaagtat catcactaat ctcttttaaa agtttctggt
     1021 cttgcacaac agttatgtgc caaggctgtt tattacatcc tgaaggtgct gcaaatgcac
     1081 agtgtaataa tgtgtttact tgctcatcac taatttgttc tgtcgtaaat tttcgtatac
     1141 ttctacgact ttgtattaaa tttattgttt ggttgtcttg aaaattatta ttcatatcta
     1201 ttcccctttt tatgtatttc ttagtacaaa tactaaatct tcagaaaatt aatcttataa
     1261 ttaaatttct ttacaatatt attataatta tttttagctt gtatgtgaag acttaaaaat
     1321 ataaacactt ttaatatatc tcaattatat atataaatga aaatctggta ttcgtcatct
     1381 atagaaactg ctcttttcta tagtttaatc aataccagat taataaaaca tgatatatta
     1441 atttaaagga ccccatccct ctttagaatg aatctctgtt tttacagact taacactatc
     1501 tgtgtcaaca tcccatgacc tacctgcaac agaaatacca tttttacctt tataccatct
     1561 accttcatca taatcgtaaa ctagggaagt ataccaatta cccccagtct tattatttgc
     1621 aaatacaact acagtagcat cagatttagt aggattatat gcatatggtt gtatatatag
     1681 tgtatcctta cttaatccat attttgcctt aaattcatct ggaattgtag gccacccatt
     1741 tttatattta ttgtctaata aatattttct aaaatcatca ttaccctttg aggctcctgg
     1801 tattatagaa gggtcttgtg caaatgatgc aatcaaatct ttcatacttt gatgtatatc
     1861 cctagccatt tcaacaccat ctggatgttt tgtacaagta acatacactt ttgcaatgct
     1921 ttctattcca gttattccat cataaccatc atcaaacgtt gctgaatata ttccacctga
     1981 cttacacttt ggttctgttt caaagtactt accatctttg ttttctaata cctctttcat
     2041 gacttcattc ttgcctttac ttgattcctc agccattgca ataacaattt gagtctttat
     2101 attttctcta ttagaaagac atgtaactgc tttacttttt tctatatttc taaacaatgc
     2161 tggaacagct actataacta aaatgcctat tatagatata actactaaca attcaattag
     2221 tgtaaaaccc tttttattca taataatcat tcccattaaa taaaattata gtacttattt
     2281 ttaaatacta catattaata aattgtctac ataaatcctt tcaacagaac tactaatata
     2341 cttacagaac tactaatata ctcaatattc aatttagtaa ttaaataact tgtatgctaa
     2401 aactcaatat cctctacctc tattttgtca atagcatcct gtactaattt ttcaatgtca
     2461 agtgctgttt cagatttttc tattttctct aatctaggtt tagtgacggg ctttctagga
     2521 gaaaatacac tacaacaatc ttcttcaggc actatagatg tttcaaaagt ccctattttt
     2581 ttggctatat caactatatc tgatttatcc attgctataa gtggtctaaa cactggtaag
     2641 tctacaacgg cattagtaca agtaagtcct tgtatagttt gagatgcaac ctgcccaata
     2701 ctttcacctg taattaaagc atcacaatgt cttttttcag aaagtctctg cgctatcctc
     2761 atcataaatc ttcttgaaag aatagtagat tcttcttcat tacaattctc gccaattgct
     2821 ttttgtattt ctaagatatt aactttatga agccttactc taccacagta ttttgccagt
     2881 attttagcta aatccttaac tttttcctgt gacctttcac ttgtaaatgg ataactatga
     2941 aagtgaactg cctctacttc catacctctt tttgcaacca tccatgtagc tactgggcta
     3001 tctatacctc ctgataaaag agacatagct ctaccattcg ttccaagtgg caatccacca
     3061 tatccaggta tagtatcact atagaccata gtatgaaatt ctctgtattc gcactttatt
     3121 ttaacttctg gatttcttac atcaacatta atcctatctc ctacctttga taataagtat
     3181 ccacctatat ccatactcat ttcttgagaa gtcaatccga aagacttatc tcctcttctt
     3241 gactctacct taaaagtttt atatccagcc tctatttttt cttctaacat ttttaaagct
     3301 atttctttta atgtatcata atctttttta gctcttactc cagggcacac acccactatc
     3361 ccaaatactt ttctaacttc ttcaattact tcttcataat catagtcttc taaatcaaca
     3421 tatattcttc catactcttt atatacatta aatcttccaa ttggttttaa catatttctt
     3481 atatttctta taagcctatt ctcaaatata tatctattct ttccttttac tccaatttct
     3541 ccatacttta ctattaatat attatacacc atttcacctc ttaaactaat ttattatcac
     3601 tatttataca gtattcttta ttttcttttc attattgacc ttaagtcaca aattgattct
     3661 ttcaaaactt tcacagcttc taacatttct tcttctgtat tcatatcaga taaactaaat
     3721 cttattgttc catttatttc ttcatttgta agacctatag catttaatac gtaactacct
     3781 ttctttttag aagaacatgc agagccagtt gatacataaa tacctttttg ctctaagtaa
     3841 tgaagtaaaa cttctccttt tgtccctcta aatgatacat ttaatatatg acatactccg
     3901 tcttctggag aattgatttt tatatcttct atattatcaa ttatttcatt ttttaatata
     3961 ttttttaagt tatctacttt cgaaataaca gcatctaaat ctttgtttag tattctaaca
     4021 gcttctccaa gaccatatat tccaggtaca ttttctgttc ccgacctaat tccaatttcc
     4081 tgaccaccac ctgttaacat aggcttaagg cgattatttt cttttatata cataaatcca
     4141 attccctttg gaccatgaaa tttatgtgca cttacactca taaaatctat attgtattta
     4201 gatggtctaa agttaatttt tccatatgac tgaatagcat caacatggaa ataaattttt
     4261 tcttttaaac ccttcaaata ctttccaact tcacctattg gctgtatagt tcctatttca
     4321 ttgttaacat gcatcatgct tactaagcat gtagttgact ttatcgcatt ttttaaatcc
     4381 tctatattta ttttcccatc ttttccgacc tctaaataag ttacttcaaa accatcttct
     4441 tctaaatctt ttaatgtatt taaaacagaa ggatgttcta tgttagtaga tattatgtga
     4501 ttttttcttt ttttatttaa actagtaata cctcttatta tagtattatt acactctgta
     4561 ccacctgatg taaaataaat ctccttttct ttcgctccta gagatttagc tatatcttgt
     4621 cttacttctt ttatagcttt ttcaacttct acaccttttc tatggacaga agaaggattt
     4681 ccatattccg tattaagtgc ataaaccatt ttatctataa cttcttgata aggttttgta
     4741 gttgcgctat tgtctaagta tatttccaac ttaattacac ctacctttat ttaaaatctt
     4801 caactctaac tattatacca ctaattatag tatttttttt gttaatcttt attaaaaaga
     4861 caatcttaaa ttataaaata ttatttcatg aaaagctagt tttttcactt tattacaaat
     4921 ttt
//
LOCUS       DAELTA010000216         4907 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.221, whole genome shotgun sequence.
ACCESSION   DAELTA010000216 DAELTA010000000
VERSION     DAELTA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.221"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..1334
                     /locus_tag="KPG13_003375"
     CDS             27..1334
                     /locus_tag="KPG13_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987062.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1420..1682
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1808..2611
                     /locus_tag="KPG13_003376"
     CDS             1808..2611
                     /locus_tag="KPG13_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0987063.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2649..3260
                     /locus_tag="KPG13_003377"
     CDS             2649..3260
                     /locus_tag="KPG13_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG0987064.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3292..3963
                     /locus_tag="KPG13_003378"
     CDS             3292..3963
                     /locus_tag="KPG13_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG0987065.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4262..4624
                     /locus_tag="KPG13_003379"
     CDS             4262..4624
                     /locus_tag="KPG13_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG0987066.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         1966 a          457 c          873 g         1611 t
ORIGIN      
        1 acataaaagc aaaggtggga ataaaaatga aagaaattgc tgatgctata acatcagagt
       61 atttaagcta tggtatcaat gaagatatta gtatctatga aggtagattt tgcatatatt
      121 tagataaaaa atatagatgt aatgggaaaa tttattataa aatgacacct ccaatatcta
      181 taagttttaa agcagacata ggttgtgttg aagagataga caatgaagat gacaatttag
      241 ctttagatta tgataatgct attcttgaag ttcatggata taaaattatt tctataacaa
      301 taaatacttt aagtgaattt agtgtagaag gttatatcaa tgatgactgt ataaaatcta
      361 agaactccta tgttgaatat gtagatttta atataataaa cttagataaa atacctggaa
      421 aactcattaa atacaatgat aaggtatacg caggtagaat tgaatttgat ataaatgatt
      481 atgttgtaac tatagacaag cgatatgatt atagaaaaga attaaaatca gaattaaagt
      541 ctaaatctgg agcaataata actcatatag gcaggattag aagaaaagat ggtagaattt
      601 ttagaacaaa taatacaata aatcttttag ataggatatc aacagctttg agttttatgt
      661 gtggaagata tgtagggttt tgtttggcca aagggtatag aagtggtaat gaagtttata
      721 gaatatggaa tgaaaatcaa atatctcctt ttagatatgt accaacatgg tcagatacac
      781 tttcaaatta tcataatatg gaaaaatata taagccttat gtgtaaaaag ctagaagatt
      841 tttattatgg ttctgctata aagagtgttg ttgattggta catagaatct cttggaagtg
      901 ctaccatgga aaacaatata atatctgtac aaattgcact tgaaactttg tcatatgtaa
      961 ttctagtgga acaaaataag atacttacag atgaagcatt tgactgtaat ttggcatcta
     1021 aaaatataag actcttactt gatacatgta agataccata tggtaaacat gaattaaata
     1081 tatttgataa tataataaaa aataaatttg atgatggagt ggatttagtt atttatttaa
     1141 gaaatagcat agttcatcca agtagaaaga ctcatagagc tgtgttagag gttgaagata
     1201 tttggaatat aatttcaata ggaacgagat atattgaact tgttttactc tttattttag
     1261 gttatagagg agaatattca aacagattag tagaaagatg ttatggtgaa gtggaagttg
     1321 taccttggaa ctagattttt atatttattt taaaatatat attgacatat attgccttat
     1381 gctataaaat caatatcata ataataattc agaataacat taaatgctaa gaaaagaaat
     1441 agtaaaagta gttaatctta aacagagagt ttccgattgg tgagaggagc aatagatttt
     1501 actttgaaca catctttgag caggactctg aaagagtaga aatactttat taggagattt
     1561 ccggaatcct tgcacccgtt atagtgctag agtataataa ttttattgta cttaaagagg
     1621 ttagtgccgt gaggcattaa tgaacagagg tggaaccacg agagttaact cccgtcctct
     1681 agcagaagct agaggcggga gttttttaat ataatttttt aagcgcgcag agaattatat
     1741 tgcaatagat acacttttta aaaaataatc aaaatatttt aaaaattaaa agacgaggga
     1801 gaatattatg aaaaatatat taaaaaaagt aggaatattt acaattatgt taggtttatt
     1861 aggtggagtt gtaggttgtt caaaaccaga taatgaaaaa gataaggatg cttcaaagga
     1921 atctaaaaaa gaagtagttg tagggtttga taatacgttt gtaccaatgg gatttttaga
     1981 tgaaaaggga gatacagtag gctttgacgt agacttagct aaagaaacat ttaaaagact
     2041 tggaatggaa gttaagtttc agcctataga ttggtcaatg aaggaaactg aattaaatga
     2101 ttcaaaaact gtagatgtat tatggaatgg atattctata acagacgaaa gaaagaaaat
     2161 agtatcatat acagaaccat atttacaaaa taaacagata atagttactc taagtgactc
     2221 aaaaataaat tcaaaagctg atttaaaaga taaagaagtt ggaacacaac aaggttcaac
     2281 agcacttgat gcagtggaaa aggataaaga ttttatgaat agcttaaaag gtggagcacc
     2341 agttctttat gatacttatg ataaagcact tagagattta gagataggta gaactagtgc
     2401 agttgttggt gatgaagttt taattagata ctatatggga caaaagggtg aagacaaata
     2461 caaggtatta aaagacgatt ttggattaga agattatgtt gtagcaactt ctaaagaaaa
     2521 tccagaactt tgtgagaaaa taaatgaaac attaaaagaa atgaaaaaag atggaacttt
     2581 tgacaagatt tatgataagt ggtttaaata gaagataagt tagctgttaa gaagggggca
     2641 ctaaaggcat gttacaaggt ttagagatag ttatagcaat gttttgtata acattgatag
     2701 tatcaattcc attggggatt ggagttgcat ttttaagatt atcaaagaat aaattagtaa
     2761 gtggtattac acaatgttat atattgataa tgagaggtac acctttactt ttgcaaatga
     2821 tagtcatatt ttacggatta cctcttttag gaattgtatt tgacagattt acagctggag
     2881 tagtagcttt cttcttaaat tatgcagcat attttgcaga aatatttaga ggtggaatac
     2941 agtcaatcga tagaggacaa tatgaagctt caaaagtact aggatttgat aagtttacta
     3001 tgtataagag agtaatcttt ccacaagttt ttaaaagaat actagcacct atttcaaatg
     3061 aagtaatcac attagtaaaa gatacatctt tagtatatat attaggatta aatgatattt
     3121 taagaatatc tcaaatagca atgaacaggg aagcaagtct gttaccatta tttgaggcag
     3181 gagcgatata tcttatattt gtagctatac ttactaaggg atttgaatta cttgaaaaaa
     3241 aatattctta ttatagataa agttacaata ttataggagg aggtttttta tatgttgaaa
     3301 atcaaaaact taaataaatc atttaaaaaa aatagagtac ttaaagatat ttcttttgag
     3361 ttagaagaag gtcagattgg agttttactt ggtaagtctg gtgcaggaaa gaccacaata
     3421 ttaaggtgta taaatggtct tgaagaattt gatagtggag agattataat agataatgag
     3481 gtaataaaaa ataagagaga tatggcaaaa atacgaggaa aaataggtat ggtttttcaa
     3541 aatttcaact tatttcctca tatgacagtc cttgaaaata taattgaatc cccagttaat
     3601 gtttttaaag ttcctagaaa agaggctgaa gagagggcta gagaactttt aagattagtt
     3661 gatttagaag ataaattaaa ttcttatcca tttgaattgt caggaggaca gcaacagaga
     3721 gttgctatag ctaggtcatg tgcattgatg ccaaaagtcc tttgttttga tgaacctact
     3781 tctgcacttg atattgatac tattcaaaga gtagtcaata taatgaacag acttaaagac
     3841 aaaggtatga caatattaat aattacacat gatgttgtct tctcaaataa tgttgcagat
     3901 aaaattataa gtataaaaga tggtatagta gaaaatgttc aaataaaaga aaaaattgtt
     3961 tagagtttta taaatataaa ctagggtaga attttataat ataaataaag ggcaagtaaa
     4021 atcaaatata ctatgccctt ttgttatctc aaaatttaat cttatatttt atgtttaagt
     4081 tacattataa tttataataa aaataaaagg actatttaaa tatataatgt gaataaatgt
     4141 ttaataattt gaaatatgat aattttttat gttgaaagct tataatgatt atgatataaa
     4201 ttggttatag tatacaattt atgggtataa caataaaaaa ttagaaagga atttttataa
     4261 aatgattaaa ttatatggat atacaaaatg ctctactgtt aaaaaagcta aaaattggct
     4321 taaggaaaat aatttagaat ttgaagatat agatatggtt caaaaccctc ctagtaaaga
     4381 ggagttaaaa tcaatttata agactagtgg atatgatata aagaaatttt ttaatacaag
     4441 tggtatgaaa tatagagaat tgggtcttaa agatattgta aaaactgaat ctgatgataa
     4501 acttttagaa atattggtaa gtgatggtat gcttattaaa agaccattac ttttggatgg
     4561 taaaaatgta ttattgggct ttaaagaaga tgtatggaaa agtactttgc taaaagaaga
     4621 ttaggaagag ataggtgtgt tgattaaaat tattaattaa tacacctata aatttatgta
     4681 ttaaatctaa tttttataaa tattaattag caaattcaaa tcaaaaaaat tcatatcaac
     4741 aaacatatta cttcttatta caaattatat catatgtttt atattaaaat attataatat
     4801 tacaacgaat tgttacaaaa ttacttatta ttaaccatga acatttgttt tgtgctatca
     4861 tatttatata agggaattgt cataaaaaat atcttgttca tacagtt
//
LOCUS       DAELTA010000217         4847 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.222, whole genome shotgun sequence.
ACCESSION   DAELTA010000217 DAELTA010000000
VERSION     DAELTA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.222"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            12..623
                     /locus_tag="KPG13_003380"
     CDS             12..623
                     /locus_tag="KPG13_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG0987067.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            921..1259
                     /locus_tag="KPG13_003381"
     CDS             921..1259
                     /locus_tag="KPG13_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG0987068.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1666..3102)
                     /gene="miaB"
                     /locus_tag="KPG13_003382"
     CDS             complement(1666..3102)
                     /gene="miaB"
                     /locus_tag="KPG13_003382"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG0987069.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(3350..4786)
                     /locus_tag="KPG13_003383"
     CDS             complement(3350..4786)
                     /locus_tag="KPG13_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG0987070.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1634 a          745 c          533 g         1935 t
ORIGIN      
        1 aggaggtaaa aatgataaac gatattaaaa ataaattcaa aaattacaaa ccatatataa
       61 atggatacaa gaatatgaag agagcatctg ttttaattcc aatagtagaa ataaataaca
      121 cacactatat attatttgaa gttcgctcaa aaaatttgaa gcatcaacct agcgaaattt
      181 cttttccagg tggtaaaata gaatcaggtg agactccata tgaagctgtt attagagaaa
      241 cttgtgagga acttggaact tttagtgaca atatagaagt aatatcaagt cttgacctcc
      301 ttataacacc agtaaagttt atcatacatc catacttagg atatataaaa aatattaata
      361 atttaaatat aaacaaggat gaagttgacc atactttttt ggttcctata aaatatcttt
      421 tagagaatcc tccaaatact tatagtaata ctgtaaaagt ttttccagat gaaaatctac
      481 catatggttc aattccaaac caaaaagatt ataaatttga aagtggaagt tatcctattc
      541 tgttttataa atataaagat tatgtaattt ggggaatgac agcaaaaata ctagaaaact
      601 ttttaaattt tttatcagaa taacactcat aataaattat caaaattttt acataagaaa
      661 tttataaaag tttctaaatt taacaattct aaaatatcaa taactatatt ttgtagcact
      721 tttttatttt attattgttt gaatatttga tggttttcaa tgcaatttca acataaatac
      781 tatatattag tgtatactgt tgatgaatta aagtatgata atctatttaa taaagtatga
      841 agtaaatgat tttaatgtgc ctttatggta aaattactaa taagtaataa aattaattaa
      901 aatatatgga ggtccttata atgtttgctg taggatttgt tgttataatt gcacttgcct
      961 ttttcatttt aagtgtacta ttatttaatg gaaaagccag tatgctacta gcaggttata
     1021 actctatgag taagaaagaa aaagcacgat acgataaaaa aaaattatgt agaagttcta
     1081 gcattgttac ttttattgtc tctataatgc tatttattat ggcgtttttg ggatatagaa
     1141 tagagagtgg tcaaatggac gaaaaaaaaa tggttccctt ttctataata tttataatag
     1201 tcatactttc cagtgtattt tttaatatcc tctactctaa caaaaaatgt aaaaaataaa
     1261 tagttgtaag ttgagcagtt aattataaaa gattgactgc tttttcatgt atattgacta
     1321 ctcttattaa atattttaac atttaaaaat ctatgtaaaa ataatgaata tagtacaaac
     1381 ttctaataat gattttacct ttgtggttac agtccactat atataattta atatgataac
     1441 agggaattat taatgacaac taagtaaact tttataaaat ataataaaac aaaaatagat
     1501 gtacaatgaa attatatctt atatcaaatt tatttttatt aatagcaaaa acttacatta
     1561 gcataacata tgacaaattc gcttaaaata aaaattttta atataatttg ttctatagta
     1621 catctatttt gattagttta cataatttta ttattttgta caatattaat tatttactaa
     1681 tatcccattt aatgaaaaag ttctaggttc agtaattttg accataacta atttaccaat
     1741 taaatcatca tttccacctt cgaaatttac gagtttattt tgtctagttc taccagcaaa
     1801 cttattttca ttatttttac ttcttccttc aactagaact tcaactattc tatctttata
     1861 tccatcattt atttctgcag aaatcctatt tacagcttct aacaccctat taaatctatc
     1921 atgctttata tcttctggta tttgattttc cattttagca gcaggagttc cctgtctttt
     1981 agaataaata aatgtaaaag ctgaatcata tctaactttt tcaaccacat ctaaagtgtc
     2041 caataaatct tcttctgttt ctcctgggaa accaatcatt aagtctgtag aaaaagcaat
     2101 acctggaact tcctttttag ctttttctat tatttctaaa taatattcct ttgtataatg
     2161 tctattcatt tttttcaaaa ggctactgct tccgcattgt attggtagat gtaaaaactc
     2221 acacacctta tcacaatctc taattgcgta tattacttca tcagatatat cttttggatg
     2281 agatgtcata aatcgaaccc tttcaattcc ttctatatca ttcacttttc ttaaaagttc
     2341 tgagaatgaa actggatttt caagtgtctt accataagaa tctacatttt gtccaagtaa
     2401 agtgatttct ttcgttccat ttgcaacaag ctcttttatc tcgttaatta tatcttcagg
     2461 agttctactt ctttctcttc ctcttgtgta tgggactata caatatgtgc agaaattatt
     2521 gcatccatac attatattta caaatgcttt taattcaaat tttctattac ttctaagacc
     2581 ttctattact tctccatcca catcccatac atccactaac attttgtcag aatttattga
     2641 ttctgtaagt aattgcggaa atttatatag attatgtgtt ccaaatataa ggtctacatg
     2701 tttatatttt tttcttaatt cctctacaac atgtggttgt tgcatcatac atccacatac
     2761 agctattttc atatctggat tttttccttt tagatgtttt aattgtccta agttaccata
     2821 tacttttagc tctgcatttt ctctaactgc acaggtgtta tatattatta aatcactttc
     2881 ttcaaccatc attgacattt ggtatcccat ctcttctaac attgaacata atttttctga
     2941 atcatgttca ttcatttgac acccaaaggt ttgagtaaac acgagttttc tctttccagt
     3001 tctgttgaag tattcttcat tttgtctgtg tatttgttct atatatttat cttgttcttg
     3061 tattttctct ataggtattg ttacttgttt tcttttgctc acgtctattc ctcctagtta
     3121 atttcttata ttacacataa ccttaattat atcaaataag aaaaatcttt taaagatatt
     3181 attatttttc tagttgtgtt ttaatagtaa atctaatgat ttatatatac tatatttcta
     3241 aaatataaaa tcatataatt attataataa taaaaagatg ttttaaatta aaacttaaaa
     3301 cacctttcct taaataaaat atatgattta ctattaaata caattacatc tattcatttt
     3361 caattaaata acaatcattt aatattaaat attgaccttc ctcaaccgtt atatatcttc
     3421 ttcctgaaaa agtatcatta gtaactattc cctctgcatt acctaagcta tcatttctaa
     3481 cttcataata tgcatcagaa gaatttgaca ttataatata ttctccagca ggaatttcta
     3541 atccaatttt atacatacca tttccaaact taccattctt ggccttctgg gctttagcat
     3601 actttgctaa tgtcattgtt gaatcctcaa ttttaatata ttgaccattc tttaaggtga
     3661 tatatctagt cccactaaaa atatcatttg aaagtatact atcagcatta cctgtactat
     3721 cacttgtaac ttcataataa gaagcataac ttcctgagtt tgaagttatt aagtattcac
     3781 ctgctgagat atcttttcca accttataca ttccatcttt tatagtcttt gtagtaggtt
     3841 taacattttt tacaacccca gtattaatat ctccaattcc atttgttata tttaaacact
     3901 gttgtactat atttgaatct atatatccta tagaagttat cttcttagca ttttttaaaa
     3961 tgcttttatt gctaccatca ttaactaaaa ccattggacc atgttttgat aaagaagatc
     4021 ctacaagtgc atttgttaat tcataagctc cagctatata aaactctcta gcatccttat
     4081 aaaacttatt tattattgct ttattagttt caaaacgagt actcccccct agtctagtag
     4141 atttagtatt gtttactaaa gtggtactca ttgaagctgt tcctcctatt acataagact
     4201 ttaaaccact agttgaaaaa ggtatacttt gtccattagt aagaattatt ggtgctttat
     4261 ctcttgctgc tacagatgca atacttatag catctgcttc tcctttatat gcattggtta
     4321 gcataacagt attgactttt tttattgaat ttatttcttt tgcaacatta taacttgttt
     4381 ttattctgtc atttccctta attcttacaa ctttcatttt cttagatttt aatgaatttt
     4441 cgacagatgt actaatagaa tatgttcctc caattatata aacttttgat acatttttta
     4501 atcttgcaga tgtctcagtt ggtatagtat ttttttcagt taatagtata ggggcattta
     4561 aagctcctgc tagtccactt gcacttaatc catctgcaat agagttacta gtattaatta
     4621 atatagcagt tttatatgca tttttatctg ctattttacc tgcgatttca tacttatttt
     4681 ttccttgtat tttttctaca gatgatattg catctactaa ttgaatattt gcaaatacaa
     4741 aagaaatact taaagtcaat gttaatattc ttttatagaa tttcataata aaattgtccc
     4801 cctcataata aatttttaat atataaatac atattcttaa attagtt
//
LOCUS       DAELTA010000218         4803 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.223, whole genome shotgun sequence.
ACCESSION   DAELTA010000218 DAELTA010000000
VERSION     DAELTA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.223"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            345..854
                     /locus_tag="KPG13_003384"
     CDS             345..854
                     /locus_tag="KPG13_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG0987071.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            882..1076
                     /gene="rpmI"
                     /locus_tag="KPG13_003385"
     CDS             882..1076
                     /gene="rpmI"
                     /locus_tag="KPG13_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG0987072.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1132..1488
                     /gene="rplT"
                     /locus_tag="KPG13_003386"
     CDS             1132..1488
                     /gene="rplT"
                     /locus_tag="KPG13_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG0987073.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1798..2343
                     /locus_tag="KPG13_003387"
     CDS             1798..2343
                     /locus_tag="KPG13_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987074.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2778..4703
                     /locus_tag="KPG13_003388"
     CDS             2778..4703
                     /locus_tag="KPG13_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0987075.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
BASE COUNT         2025 a          483 c          909 g         1386 t
ORIGIN      
        1 atatatagag tcatgtagaa tatacatata taaaaggatt ttgggtataa atttaaaaat
       61 taatagcaaa atgagtagta tagacatata attagtagta ggaagtaatt aaatttttcc
      121 taaacaaaaa aaatagttgc aaataatttg attaagtgat atagtaaata aggtaagaat
      181 aaaacaaaga agaagtccgc ttctcacctt acgccggagg ttagtaaggt aaatataagt
      241 taaagcttaa ttttaattta agtttgttta aatttggtaa cttatattgc ggatgattac
      301 gtcatccgct ttttatttta taaaaatttt ggaggtgtcc tacaattagc aaagaattag
      361 caatcaatga acaaataaaa gataaggaaa taagagtcct ttcaccaaca ggtgaacaac
      421 ttggagtaat gcctactaaa gaagctcaag caatggctaa tagcaaaaac ttagatttgg
      481 ttcaaatctc gccgaatgca aatccaccag tatgtaagat aatggattat ggaaagttta
      541 gatatgagca agcgagaaaa gagaaagaag ctaagaaaaa acaaaaaact atagttgtaa
      601 aagaagtaag acttagacca ggtattgaac aaaatgattt aaatacgaaa gctaataatg
      661 ctataaagtt ccttaaaaaa ggggataaag tcaaagttga acttagattt agaggaagag
      721 aattaggaca taaagacata ggtaaagaag ttatgcttaa gtttttagat ataataaaag
      781 agtttggaga accaacaaaa gcccctgcat ttgagggtaa taatatggtt gtaattatag
      841 atccaaaaaa atagatgatt aactgagagg aggaatttgt catgcctaaa atgaaaactc
      901 atagaggtgc agcaaagaga ttaaagaaaa caggaactgg aaagttaaag agagctaaag
      961 cttttaaaaa acatatatta actaaaaaat ctgcaaaaac taaaatgaac ttaagaaaat
     1021 caactttagt aagtgatgga gatgctaaga gaatagcaca attattacca tactaagata
     1081 cagattgtaa ataataacta tagaagaggg atataaaagg aggtcataac gatggcaaga
     1141 gttaaaaaag ctatgaacgc tcgtaaaaag cataagaaaa tattaaaact tgcaaaagga
     1201 ttcagaggat ctagaagcaa attatataga ccagcaaata cattcgtaat gaaagcatta
     1261 aaaaatgctt atataggaag aaagttaaag aagagagact tcagaaaact ttggatacaa
     1321 agaataaatg cagctgctag aatgaatgga atatcttatt caagattaat gaatggatta
     1381 aaattatctg gtgttgaagt taacagaaaa atgttatctg aaatggctat acaagaccca
     1441 gaaggatttg caaaattagc agaagttgct aaagcaaaat tagcttagta ttgaatcatg
     1501 agatgtaaag tctttgagtt atataaataa ttcaaggact tttttatttt ttgaaaaata
     1561 cttattagtg tcatgtacaa ccaattttat aatgataaat aaaataagca atacaataga
     1621 attttatgag atttattata agaattgtta caattattta tgatagctat aaaaagtcat
     1681 agtaaaaatg atattgatgt atgttgatat atgtataaag tttccaatca atcttgaatt
     1741 aatattatca acatgataat attatcttat taataatata aataaggggg acataatatg
     1801 aatgaaaaac taatggattg ttttgtaaac ccaattaaat gcaagttatt gattgaaatt
     1861 ttttcaaata atcgaactac agctaaacaa ttaggagaaa aatttcaaga tatatcaaag
     1921 gcaacacttt atcgttacct taataaaatg ttgaaagatg atattataaa agtggttgaa
     1981 gaaaataaaa taaggggaac tgttgaaaaa gtatatgcta tgaagttaga tatagctgat
     2041 gatgtgaaca aaattataga tgagaactct ggagaagaat atgctcaact gtttactcaa
     2101 tatatgatgg gatttttaag agaattttat gaatacgctg aaagaaagga aaaggatatc
     2161 ctaaaagatg gttcaggatt ttctgtctgt ccagtatatg ctagtattga tgagatagtt
     2221 gaagtgagta aaaaaattgc tagtatacta gaacctctaa gaaataactc acatgaagaa
     2281 ggtagaagaa tgcattctat aggagtgata attactccac ctaaaaagga tgaagggaaa
     2341 taaatctgat ttacatgggg tagagccaat ttatacaggg aatcgtttta tgatttacat
     2401 atgtatccag agcaaagtat ctctgcttgg attgtacttc attgtaccat ttcatccaat
     2461 gacttggagt ggtacaatga agttttttaa tttttcatat taatgctaaa gaaatgaact
     2521 ttagaccaca tatgcaatta ggttgcatta tacaattaga ccacatatac aaaataaaaa
     2581 tcagtatttt tatagcagat aaattgactg tcattcttct taaaaatcat ctattagtta
     2641 ttgttagcat attgaaagaa attatagtaa tctattgaat aaatgaatat tgcctataat
     2701 catataaact gctatagttt aaaataaagc aggacgttaa attttatagt aatctaaaaa
     2761 tttaagaggg ggattttatg aaaataaaat ttgttattat gccattggtg atatttttaa
     2821 tggtattatt tgtggggtgt caaagtacaa aagatacaga agttaaagca aaagatacag
     2881 aaattaaaat attagggaca ggtgatttac attcaatttt gacagactct atggtttcgt
     2941 atgtaaatga ggaaagggaa aagaataaaa atctgcttat ggtagatgcg ggagatttct
     3001 atggtacaca aagtagagaa atgtgggaat ggtcttctgg aaaaaagtta attaatatta
     3061 aaagaaatgg tagagcagag tatgatgaca taattaagag tagtaaagat gaagtaccta
     3121 tagttaaaga tatggcaaag ttgaaatatg atgctgtagt tttgggagat aatgaatttg
     3181 tttcaaatga taagcaaagt cttgataaac tggtttctga ttttaaaaat aataatatgc
     3241 cattggtatc agcaaatata tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa
     3301 tgaaaaatat taagacagat gagggcaatg taaaagttgg aatactaggt cttactataa
     3361 aagaagtagg ggaaaccctt ggtcttgagg attttgaaaa ggataaaaaa gctagagagc
     3421 ttggagaaca agctgattac aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa
     3481 aaaaatggat aaaagttatg gaaaaagaaa gtccagacat aatagttgca gttgtacact
     3541 caggggaaga acctaaaaca gatagaaatc caggaaatag aataaaagag ttagcaacta
     3601 cagtggatgg aatagatgca attgtagctg gtcatactca tgaaaaaata gatgaacaca
     3661 aatataaaaa taaatctgga gatgaagtaa ttgttacaca accaggttgg catggagata
     3721 gagtatcaga gattaatttc aaactaaata aagagaatgg aaagtgggac ataaaggaaa
     3781 agtctagtaa attaaaaata attgataaag aagtaagtat agttgctact gcagatttac
     3841 attcacattt ctctgataaa ttaacagttg accttgttaa tgaacgaagt aacccaattg
     3901 aacctgttgt agtagatgca ggcgacttct tagaccctca aacagatgaa atgactcaat
     3961 ggtataaaga gtggaaaagt ataagagata ataatagtga taaaacgatt agtagatgcc
     4021 caatggtcat ggatatgaat cagggaatgt atgatgcagt agtacttgga aatcatgaat
     4081 ttgtatctga aaaagatgaa ttttggtcag atgaacgcct tttagatgct gttatagagg
     4141 attttgaaca ggtggcaatt tctgtgttat cggcaaatat atataggcaa tctggtaaaa
     4201 attatgttaa accatatatt ataaaagatg tggagacgag tgaaggtaat gtaaaagtag
     4261 gaatattggg acttactata aaagaagtag gtaaaggtct taaaaatgta aacttacaag
     4321 agcaatttca atataaggat aaattgtatg caaatgattt agtggaagat gcaaagaaat
     4381 gggtaaaagt tatgaaaaag aaaaaaccag atataatagt tgcagttgta cattcaggag
     4441 aagaaccaaa aaatccaaaa catccaggaa atagaataaa agaactagca actacagtag
     4501 atggtattga tgcaattgtt gctagtcata ctcatgaaaa aatagatgag catgaatata
     4561 aaaataagtc tggagaaaaa gttatagtga cacaaccagg tgagcatggt aaatattatt
     4621 ctaaaattac ctttaaagta aataaagaaa agggttcgtg gactattaat gataaattca
     4681 gtaaaacaat tgaggttgaa taactttata aaatttatag ataaagtaaa ttatctgata
     4741 ttggcatgat atataagata aagtttctaa tattagtata atgcataatg tgaaatattt
     4801 taa
//
LOCUS       DAELTA010000219         4671 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.224, whole genome shotgun sequence.
ACCESSION   DAELTA010000219 DAELTA010000000
VERSION     DAELTA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.224"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(327..794)
                     /locus_tag="KPG13_003389"
     CDS             complement(327..794)
                     /locus_tag="KPG13_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG0987076.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(873..1415)
                     /locus_tag="KPG13_003390"
     CDS             complement(873..1415)
                     /locus_tag="KPG13_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987077.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(1467..2153)
                     /locus_tag="KPG13_003391"
     CDS             complement(1467..2153)
                     /locus_tag="KPG13_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG0987078.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(2153..3013)
                     /locus_tag="KPG13_003392"
     CDS             complement(2153..3013)
                     /locus_tag="KPG13_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987079.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            3148..3948
                     /locus_tag="KPG13_003393"
     CDS             3148..3948
                     /locus_tag="KPG13_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG0987080.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            3973..4542
                     /gene="yfcE"
                     /locus_tag="KPG13_003394"
     CDS             3973..4542
                     /gene="yfcE"
                     /locus_tag="KPG13_003394"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG0987081.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1685 a          728 c          498 g         1760 t
ORIGIN      
        1 ttttaataga gctaaataat catattttta tctgactatt ttttattttt aaatatctta
       61 catctataat agtatcactc aaaggataat ttttcaataa tttctaatta tttttttaac
      121 aatatttatt acattttttc cacttttttg tcattttttt attgtttcga aaaaaaatca
      181 gaatttttta actttatttt ggattattag tttaaattag attttcctct tagatttata
      241 aatatttaca caaaaaaact acctctgtat agattgtata cagaggtagt tttttactaa
      301 ttcctactat agtttactat atttttttac caattcttac aaacatctac ccatttttca
      361 cacttagaaa acttctccat ttcttcacat gtaagttcta ttgcagagtt actactgcca
      421 catgcaggaa atactgtatc aaatcttttc attgaatcat ctaaatagac ttttatagaa
      481 ttttttaaac caaatggaca gactccacca atagcatgtc cagtaaactc caagacttct
      541 tctggtgtca acattttagc cttacaatta aattcagcct tatacttctt attatcaatc
      601 ttagcatcac cagctgtcac aattaaaatt gcatcatctc ctattttaaa tgagagtgtc
      661 ttagctattc ttgctggaat aaccccagct gcttcagctg caagttccac agtagcacta
      721 gattgttcaa attctaatat actgtcttcc ttgccaaatt gtttaaaata ctctcttaca
      781 ttacttattg acattaaata cccctcctag tctgaacttt tataaatttc atattattct
      841 taattatatc tataattgat ttatttttca aattattcca taatattagc attccatgta
      901 tcattatttt ttccaactac tagataaagt ccttttgcca ttggaatatt gacaatatat
      961 tctcctttat actctacact cataccagat tcattaaagt acctataatc aaaatttagt
     1021 ccttttggaa ttacaaaatc aacataacct tgattatttg gttttattcc atatttcaga
     1081 gttctttcag ttatagaatt tttttcatta aataaatcta catttacata tgcagaacac
     1141 ttattcattc cctttttatt ttccacacaa ttttcaaact tcattttagc actgtttaat
     1201 ttataactat atttactatt attgcttatt ttaaatctta caatagtacc aacatctcta
     1261 cttctcctgc tatcaaaatc aaaaacaatt tcctctaaat gatttatttc ataatctttg
     1321 acacttacca tatctctata tactaattta tttatagcta tgaatagtgt aaaaatcccc
     1381 aaaataaaaa atgaaacttt tataaacttc ttcattttta gtctccttat attttgtgct
     1441 attgtgctat aatattaatc aaaattttaa taattaaact tattcttttt tgaaatctta
     1501 atagaaaaat acattgatat tataaaaatt attattgtta aaaatgtcat actatataaa
     1561 tttatatact tagtattagc tatatcagtt acataatttg aaagatatac tcctccaact
     1621 ttactataat taaaatgctt tacttgatat acaatcatac taatcatctt caaaagactt
     1681 ccatatacaa agtaaattaa aatacttact ataaaaccac aaactttcat ttttccatat
     1741 ccaaacttaa aaattaaagg cagtaaaatc gtcaaaattg ctaaaaatat atttacagaa
     1801 aaaaatacat catttaaaac catatttcta aagaacacta gtgatgtcaa tttacttagt
     1861 aaggacacaa ttattgttac agaaattatt aatcctacac ctaataaata ttttgagtaa
     1921 actatatctt ctatactaac tggcatactc cttataaaaa tccttgaatc attttcataa
     1981 tcacactcaa atgtatttat aaatatcaaa taactaacaa caatacttac tgtataatat
     2041 gaaattgaat taaggaaaaa ataaaacaca aaaaatagca atatgtactt taaattacgg
     2101 ataccatctg aactaatccc tattattaaa tcttttctta ttaaattgaa catcatctca
     2161 cctcatttac ataataatac attagttctt ctatagtagg atttttgacc ttatgcttat
     2221 caatttctat caataggctt tttactttca agctatctcc aactttaaca agtgcctcat
     2281 tataatattt atactctttc acaccaacta tgctttcttt aattgcttct aactctgctt
     2341 tatttgccct aattatctta tattcttgtt ctattaattc tttcttaaca gtaaaaatct
     2401 gatttcctct atttataaaa acaagataat cagcaatttt aattaaatta tcagtattat
     2461 gagtagaaat tattacacta ctacttccat ttttcacaaa atcttgtaag tactgcatta
     2521 tttcagttct tacaattggg tctatcccag cagttggttc atccaaaatc aataacttag
     2581 cattgtgact aagtgcactt gataacataa gcttttcaga ttgaccttta gataaatttt
     2641 caatatgcat attttcttct atatcaaact tattaaggta ttcatcaaac ctttctctat
     2701 caaaattctt ataaaataaa gaaactattt ttctaaattc cttaaccttt aaatgcggat
     2761 aaaattgtaa agaatcatat acaaaaccaa tattgttttt aaagcttatt ggagaatttt
     2821 caatagtttc attacaaagt tcaatttcac cttcatctgc atcaataatc cccattataa
     2881 tttttattaa agtcgttttt cccgaaccat ttggtcctat aatcccaaca atatcccctt
     2941 cctttaactc taagtttata ttatttaact tgaattttcc taattttttt gtgatattcc
     3001 ttatgctaaa cacatcttca cctcttatgc atttttttat acccacctat acatatttta
     3061 acacattcac tacaaaggtc atttataaat ttataaatta agaattataa aactatgtta
     3121 taatacaaag tatgaagagg tgaatatatg aaaagaatta taactataaa tacaaaccaa
     3181 ttaaataagt cccttgagac taaagaactc ttaactagaa aacttatcaa tgctggcttt
     3241 gaagtgtatt cagatattta tccagataca gagcttatta tatcaattgg tggtgatggc
     3301 tcttttttaa gaacagttag agatttcgat tttcctgaaa ttcccataat gggaataaat
     3361 acaggtcatc tggggttttt tcctgatatt ttaccagata agattgatag ctttattgaa
     3421 gcttatacaa aaaaagatta tattattcaa gaaatgtcac ttcttaatgc ggaagtatat
     3481 acaactacta gtggttctaa tatgcttgct gtaaatgagg ttgttataag aggagacaag
     3541 tctaggacta tacacctaaa tttaagcctt gataataaac atatacaaaa ttttagtgga
     3601 gatggtatga ttatttctac ttccacaggt tcaacagcct ataactattc tgctggaggt
     3661 agtattgttg atataaatct tgaacttatg cagattacac ctttacatcc gataaatacc
     3721 aatgcatata ggtgctttac atcaagtata atatgttcaa atgaatctgt tataaagatt
     3781 gcaccagaat atagatttga agattctgta ttgattgtcg ttgatggagt tgagcacaga
     3841 tttagacaaa tcgaaaatat aaaagttagt atatctgatg caaaaataaa attacttaga
     3901 atgtcaaact atgagttttg gcatagagta tctgaaaaat ttttatagac tgtacttagg
     3961 aggattttta atatgaaaat aggaataatg agtgatactc atggtagctt actttacttt
     4021 gaaaaagcgt taaatgtatt atctgattgt gatgttttat tacatggtgg agatgtctta
     4081 tatcatggac ctagaaatga tattccagaa ggatataatc ctaaaaaatt tattgagaca
     4141 ttaaataaat tagaaaacat tgtgattgta aaagggaact gtgatgcaga tgttgaccag
     4201 atggttatag aacatcctat tcagagtcct tatgtcatga gccaatttgg tgaaattaga
     4261 attatattga atcatggtta tatagaatca gaagaagaaa ttattgacaa agcaaagaaa
     4321 atgggtggtg acatattagt tttaggacat actcatgtaa aaaagctata catggatgat
     4381 aatttaatag taataaatcc aggaagtact tctatcccaa aagatggaag ccattctgtc
     4441 gcaattatag atatcataaa aactgatgat gaggatgagc ttgaacttga tataaactta
     4501 attgacatca atactggaaa tataataaat atcaatgatt agtaaatttt taataaaaat
     4561 aaaataagct ttaatttatt aaactcataa taaaaacaag taaaccattt tatttctatc
     4621 tcaattagaa gtttaagtac aataccttaa acttctaatt aaatagaatc t
//
LOCUS       DAELTA010000220         4621 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.225, whole genome shotgun sequence.
ACCESSION   DAELTA010000220 DAELTA010000000
VERSION     DAELTA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.225"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..391
                     /locus_tag="KPG13_003395"
     CDS             23..391
                     /locus_tag="KPG13_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0987082.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            394..765
                     /locus_tag="KPG13_003396"
     CDS             394..765
                     /locus_tag="KPG13_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG0987083.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(910..1365)
                     /locus_tag="KPG13_003397"
     CDS             complement(910..1365)
                     /locus_tag="KPG13_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987084.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1404..2669)
                     /locus_tag="KPG13_003398"
     CDS             complement(1404..2669)
                     /locus_tag="KPG13_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG0987085.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            3139..4590
                     /locus_tag="KPG13_003399"
     CDS             3139..4590
                     /locus_tag="KPG13_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG0987086.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
BASE COUNT         1675 a          578 c          730 g         1638 t
ORIGIN      
        1 acttaatcaa aatgaggtac ttatggaaaa cttggttaaa atatttaaag cattgagtga
       61 tgaaactaga cttaacatac ttatattagt atccaagaga aatatatgtc aaaaggggat
      121 ttctaaatat ctagggattt cagattctgc tgtttcacaa catataaaaa tattaaaaga
      181 tgttgggata ataacaggat ataaagaagg atactatgtt ttatatcata ttaataaaga
      241 atcatttaat atatgtgtag attttataaa ttctatgtta agtaattcta gtgaaagctt
      301 tatagatgta tttgatgtaa atacaataca tctaggatgc agtaaagaat gtaaatctat
      361 aaaaaaatgt tgtaagagaa gggagaaata ataatgaaag tatgtatacc agtagaggag
      421 aataaaggat tggatagtaa accatatgga cattttggtt ctgctccaat ttttgttgtg
      481 tgtgatttag aaagtggaga agttaaatct ttagataatg gagatttaga tcatgaacat
      541 ggaaaatgtc aaccattaaa agcattgtca gggacagctg tagatgcagt agttgttgga
      601 ggaataggac aaggagctat aattaaatta aatggaatgg gaataaaagt ttacagagca
      661 gaaggtgata ctataagtgc taatttagac ttacttaaaa atggaaaatt agtagaattt
      721 ccatcagacc atacttgctc acatgacgga tgtggacatc attaaaaaaa aacagctgat
      781 gaaatatata cttcatcagc tattttttct tttaataaat acgattttat tcttttaaaa
      841 ccaaaattta gatttttatg ttttttgtag tatccttaat aatatttcaa gtttacaagt
      901 ttactacttt tagatttttt ttgtatgttt ttctatatag tcaatatcat ttaatatgtc
      961 attgagttct ttattaagtg tttttaaatc atcaccatct acattgtagt ttaaaaatag
     1021 ttcctctaca ttatcaatga tagaattgac ttttctagta acttcaacaa atgtcttaaa
     1081 gttactacta tttttagcat ttagaaattt ttttaaatcc tttgatataa atgatactat
     1141 ttcatattca tcattaaata gacctctacc aataggatat ggttcaattc tctgtaaaaa
     1201 atattggtct ttagcattaa tattataagt ataagatatt tcccaaccgt ctcctaaatc
     1261 aaacttgata aaatataatg tactacttac ttttgatatt cttgtggcac caagctgtct
     1321 taaaagaact tcaatcgaat caccttgaac agtattaata ttcatatata cacatcccct
     1381 ttagtattat tatactatat tttttaaaat tttataatta aataaacagt agatataata
     1441 atagatataa tcataagtgg aaaacctatt ttgaaatact ccttaaaaga gataggatga
     1501 ccgtgtttat tacctatacc tgctagtaca acattagcag atgctcctat tagtgttcca
     1561 tttcctccaa gacaagctcc aagagatgtt gcccaccata gtggcattac atcaatacct
     1621 tcagcttgca tagtcaaaat aagaggaatt agtgttgcaa caaatggaat attatctaag
     1681 aaagaagaga ctatagctga caaccataat atcaacaaca ttgtaaatac aagatgtcct
     1741 tctgtaagtc caatcaatgc ttgagccaat ttgtttatta tcccaacttc tactaatcca
     1801 ccaactacta caaataatcc cataaagaat aaaattgtag accattcaat acctgccata
     1861 atttcatcta catcttgttt tcctattaaa agcattatag atgctcctgt cagtgctaca
     1921 acagaagaat ctatgtggat tgtactgtga aacataaaac caaaaagtat gaatgctatt
     1981 aatataaggc tttttataag tagaggttta tctttaacag attttttttc atctagtttt
     2041 aatattgcat ttttagctct ttcattcaca acaagttctt ttccataaat aaatctaaag
     2101 catattatag ttatagcaag tattactata acagcaggac ctaaatttat tacaaaatcc
     2161 ataaaactaa gattggcagc actaccaatc ataatatttg gagggtctcc tataagagta
     2221 gctgttcctc ctatatttga agctaaaatc tgtgttatta aaaatggaac tggattaagt
     2281 cctaatattt gtgtaataac tatagtcatt ggtccaataa gtaagacggt tgtaacatta
     2341 tctaatacag ctgataaaat agctgttata atagcaaaac aaatcattat cttccatggg
     2401 tcacctttgg ctttctttgc agtccagata gctatatatt caaataaccc cgagtttttg
     2461 acaacagcta caaatagcat cataccaact aatacaccaa tagtattgaa atctatatgg
     2521 cttaatcctt catcaagtgt aagtatattg aatattatca ttaatagtgc accaccaaca
     2581 gctgctacgg atctgtttac cttttctgta ataatagctg ccataactat taaaaatata
     2641 catatagcta ttatttgttg aaattccata atataagtac ctccatttgt attattttgt
     2701 gcctaatgct agtactatat atagtattac ttttttattg ttttttcaat ggatgtatga
     2761 aagtatatta tttaacaaag tttttggaaa attgacagaa tttaaagaaa atttttgtta
     2821 tttaaataaa ttttaaaatt attccattga taataatatt aaacgacaaa cgaaaaaata
     2881 taccctttaa actagaattt attgtatcta ataacaatta taaaatatta aatatatcta
     2941 tattataaaa acaataaaaa aaggtttgat ttgaatataa aactgcaata tattaaaaaa
     3001 attattcatt ttaaaccttg taatttgatt ttataaatat tataattaaa ttaatgaatt
     3061 tttaacaagg aattattcat ttttttgatt ttaagattta ttgagaaaac aaaactaaat
     3121 aggggggtaa ttaatataat ggattttttc aatggtattt taacgagtat taacaatgca
     3181 gtagtatatg taaacaatta cttatggtcg tatatcttaa ttataatgtt agttgtaatt
     3241 ggtatttact ttactattaa aacaaatttt gtacaattca gatattttat agaaatgttt
     3301 agacttctgg gtgatggtac agcgaataaa aatgcaaaaa aggaaggaaa aatctcttca
     3361 tttcaagcat tttgtataag tacatcttca agagttggaa ctggaaatat agctggaata
     3421 gcaattgcag tggtagcagg aggacctggt gctgtatttt ggatgtggct tatagcatta
     3481 attggttctg catcaagctt tgttgagagt acattagctc agatttacaa agtgaaagat
     3541 ggtcaagctt ttagaggtgg accagcgtat tatatggaac aaggtctaaa taaaaaatgg
     3601 atgggtattt tattctcaat acttataact atatgttatg gttttgtatt taatgcagtt
     3661 caagcaaata ctgtttcact tgcatttaat aatgcatttg gaataagtaa aatggctatg
     3721 ggaataggtc ttggaatttt aactgcaata gttatttttg gtggagtgca tagagttgca
     3781 aaagtttctg aaataatagt tcctgtattt gcaggattat atatattagt tgctttggct
     3841 atagttgtaa tgaatataac agagatacca agtgttatag cattgatagt tgaaagtgca
     3901 tttgatttta aaggaatggc gattggtaca tttatgggag ttgtaatgac tggagtgaag
     3961 agaggtctat tttctaatga agcaggtatg ggtagtgctc ctaatgctgc tgcaacagca
     4021 catgtaactc atcctgtaaa acaaggttta attcaatctt taggtgtttt tacagatact
     4081 ataataatat gtagttgtac agcattcatg gtattattat attcaggtta tagtagtagt
     4141 ggagcaactg gaattgaatt agctcaagaa gctttaactc atcatatagg accagttgga
     4201 aatatattta tagcagtttg tatattcttg tttgcattta gttctatagt tggtaattac
     4261 tattatggtg aatcaaatat ggagtttatg agtggaagta agactaaact taatgtattt
     4321 agagtttttg ttgtaggtat ggttttattt ggttcactta ctcaagtgga tgtagtttgg
     4381 aatcttgcag acttatttat ggcattgatg gcgataataa acttaatagc aatagcactt
     4441 ttaggaaaat atgcatttat tgctttaaaa gattatacaa gtcaaaaaaa atcaggaata
     4501 aaagaccctg tgtttattgc agatgaaata gaaggcctag aaaaggtatc agagtggcat
     4561 agggaattat ctgaggaaca tttaggataa aaaatagtat aaaattataa aaccattttg
     4621 a
//
LOCUS       DAELTA010000221         4539 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.226, whole genome shotgun sequence.
ACCESSION   DAELTA010000221 DAELTA010000000
VERSION     DAELTA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.226"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    129..363
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            413..1432
                     /gene="pheS"
                     /locus_tag="KPG13_003400"
     CDS             413..1432
                     /gene="pheS"
                     /locus_tag="KPG13_003400"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG0987087.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1449..3842
                     /locus_tag="KPG13_003401"
     CDS             1449..3842
                     /locus_tag="KPG13_003401"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG0987088.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3867..4457
                     /gene="zapA"
                     /locus_tag="KPG13_003402"
     CDS             3867..4457
                     /gene="zapA"
                     /locus_tag="KPG13_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG0987089.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1797 a          522 c          924 g         1296 t
ORIGIN      
        1 agttaattat gcattagata tatattatat aaaaaataat taaattaatt atgtattgga
       61 tatatattaa aaatagtcac ataatttgaa tggaaatgat ataatactaa aataaacaat
      121 ataatattgt aaatgcaatg aaagaggaaa gtattttgat taaacttagt aaagagataa
      181 acacctaggc tgggagtgtt ttctaagagg tcatgagaag ttccctctgg agtaacagag
      241 ctgaaatttt acagtaggct ttgacgtcaa aaacgcgtta agtttgttag aggtggtttg
      301 atgattttta aattgttaaa ctactagggt ggtaccgcga aactatagcc tttcgtccct
      361 agacagggat tgaggggctt tttttataca aaaaaacgaa agggtgatat gtgtgcaaga
      421 aaaattactt gctttacgtg aagcagcttt ggctgaaata aaagaagcac aaagcataga
      481 aagtgtagaa agtttaagag ttaagtactt aggaaaaaaa ggtgagataa ctgccatact
      541 taaagaaatg ggtaaattat ctgctgaaga aagaccagta gttggtaagg ttgccaatga
      601 ggtaagagaa aacattgaac ttagcataaa ttctaaaaaa gaagaaataa atgctattga
      661 aaaagaaaga aaattaaaag aggaagtgat agatgttact caaccaggaa aagttttaaa
      721 ggttgggaag aagcatccaa taactcaaat tatagatgaa gtaacagata tatttatcgg
      781 aatgggattc tctatagcag aagggccaga agttgagact gttgaaaaca actttgacgc
      841 attaaacgct cctaaagacc atccatcaag agatatgagc gatacattct atatcaatga
      901 tggggtatta cttagaactc aaacatctcc agttcaagta agaactatga gaagtcaaga
      961 gttaccaata aaagtaattg caccaggtag atgttttagg tcagactcgc cagatgctac
     1021 acactcacca atgttccatc agatagaagg gcttgttgtt ggaaaagatg ttactatggc
     1081 agaatttaaa ggaactatgg atatcttcgt tgaaaaattg tttggttctg atatcaaaac
     1141 taagtttaga cctcacaact tcccatttac agaaccaagt gcagaggttg atgttacttg
     1201 tttcaaatgt ggtggtaaag gttgcccaat gtgtaaatat gaaggttgga tagaaatatt
     1261 aggttcaggt atggttcatc caaatgtgct tagaaattgt ggaatagacc cagaagttta
     1321 cagtggattt gcatttggag ttggggttga aagacttgca atgcttaaat acgaaataga
     1381 tgatattaga ttattattcg aaaatgatat gagattctta aatcaatttt aattaggagg
     1441 gtatgtagat gttagtatct ttaaaatggc ttagagacta tgttgatata gacatggatg
     1501 taaaagagtt cgctgataaa atgacaatga caggaactaa agttgaaaca atagattatt
     1561 atggtgaaga aatagaaaat atattggttg gaaagatttt agaaataaaa caacatccaa
     1621 atgctgataa gttggttgta actaaagtag atattggaga taaagttgtt caaatagtta
     1681 caggagctac aaatatatca gaaggagatt atattccagt agctgtaaat ggttctaagt
     1741 tacctggagg agttgaaatc aaacagactg atttcagagg tgaattatca gatggtatga
     1801 tgtgttcagc agctgaacta ggtatagatg aacattacat tgaggagtat aaaagaggtg
     1861 gtatatatat tttagaccac gaagattctt atgaattagg aaaagatata aaagatgttt
     1921 taggattaaa agatgcttta atagattttg aattaacttc aaacagacct gattgtaaat
     1981 gcatgatggg tatagctaga gaagcagctg caactatagg aacaaaagta aaatatcctg
     2041 aaatcgaagt aaaagaaagt gacgaagaga tagatttcaa agttgagata gataatccag
     2101 atttatgtag aagatatgtt gctagaatgg ttacagatgt aaaaatagaa ccttctccat
     2161 attggatgca aagaagactt acagaagcag gagtaagacc tataagtaac atagtcgata
     2221 taacaaactt cgtaatgtta gagcttggtc aaccacttca tgcttttgat ataaatcaag
     2281 tagagactgg aagaatagta gtaagaaatg ctaaagatgg agagaaactt gtaacattag
     2341 atgatgttga gagaacatta gacaaagata tgctagttat aacaaatgga gaaaaatcac
     2401 ttggtttagc tggtgtaatg ggtggtgcta actcagaaat aacttctaat acgaagactg
     2461 tactttttga aagtgccaat ttcaaaccag aaaacataag aatgacagct aaaaaagttg
     2521 gtattaggtc agaagcatct tcaagaaatg aaaaagactt agaccctaat cttgcagaga
     2581 tagcagcaaa tagagctgca caacttgttg aaatgttagg agcaggaaaa gttttaaaag
     2641 gtgttgtaga tgtatatcca aataaaccag aacctaaaaa attggtagta aatcctcaaa
     2701 gaattaacca cctattaggt gtagatgtac caatggagca gtttgtagga attttagaat
     2761 cattagagtt taaatgtaat ttggtagcta atgataaatt agaaatagat gtaccaagct
     2821 ttagaacaga tatggaacaa gaagctgatg tatgggaaga aatagctaga atttatggat
     2881 ttgagaatat accttctgta caattagaag gaaatacgac agcaggaatt aaaacttcaa
     2941 aacaaaaatt catggatgct ttaaaagata attcaactgc tgtaggatta aatgaaatat
     3001 taacatattc atttgtaagc cctaagggag tagataagat aagagtacca gaaggtaatg
     3061 caaagagaaa ttttgttaaa ttacttaacc cattaggaga agaaacttct gtaatgagaa
     3121 ctactttaat accaaatatg ttagatgttt tatcaactaa tgtatctcat aaaatagaag
     3181 aagtatctgc atttgagtgt ggacatatat tcatcccaca agattcagaa ttacctaagg
     3241 aagaaaatag aatgtgtgta ggtatgtatg gtaaagatgt tgacttcttt gcactaaaag
     3301 gaactataga aactatactt gtaaatgtcg gatttaaagg ttatgaaatt gaacctcaag
     3361 ataataatac taccttccac cctggtagat gtgctaaaat agtgtataat aataaatatg
     3421 taggtacatt aggtgaattg catccagatg ttatagaaaa ctacaatcta ggtcaaagag
     3481 tttatgtcgc tgaaatagat attgactttg tatttgataa ttctgataga acaaagaatt
     3541 atgtgccatt accaaaatat ccatctacat caagagatat agctcttatt gttaaagatg
     3601 atgtatttgt aaaacaaata gaagatataa taaaggaaaa tggtcaagga ttagtagaaa
     3661 gctacaagtt atttgatgtt tacaaaggtt ctcaaataga agctggatac aagtcaattg
     3721 catattcaat aacttataga agtaaagata aaactctaac agatgaagat gtagctaagg
     3781 tgcatgataa gatattaagt gaattaagcg aaaaattaaa tgctaactta agatcaaatt
     3841 aacaactaat gtcgggggag gttaggatga acaaagtaat ggttaaaatc catggcgctg
     3901 aatatccaat ggttggagac aaatcagaga agtttatgat tagtattgct gattttgtag
     3961 acaaagaaat ggataaaata acacgtcaaa acccgaaatt gagcttatca gttgctgcga
     4021 tattaactgc attaaatata tcagaccttt tatttgaatg ttcagatgag aatgagaagt
     4081 tgattaaagc gaatgaagaa ttgagtaaga aggttggagc atctaatgaa gagcttcaat
     4141 tagaaatcaa atctttaaag cttacgatag cagaaaaaga agcagaaaat cgtgaagctg
     4201 aaactaagat gaaagagtta attgagatta tagaaaataa aaagcaagaa atattcgaac
     4261 ttagtaatac gacagaaggt tctagagcag agttagatgc ttataaaaac aagattgagg
     4321 agttaagtac tcaacttgaa gaagcaaatg aaagagctac tatagctgaa aacttagctt
     4381 ctgagtttca aaacaaagct tatgatttac agttaaaatg tacagggtta aataatgatg
     4441 caaaaaatgt ggaataaact aaaagatttg ctgtctcttg ttttttgaga cagcttcttt
     4501 taatgtataa atataaatgc tatataaata tagataatg
//
LOCUS       DAELTA010000222         4514 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.227, whole genome shotgun sequence.
ACCESSION   DAELTA010000222 DAELTA010000000
VERSION     DAELTA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.227"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            222..1361
                     /locus_tag="KPG13_003403"
     CDS             222..1361
                     /locus_tag="KPG13_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG0987090.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1387..1575
                     /locus_tag="KPG13_003404"
     CDS             1387..1575
                     /locus_tag="KPG13_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987091.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2359..4143
                     /locus_tag="KPG13_003405"
                     /pseudo
     CDS             2359..4143
                     /locus_tag="KPG13_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1939 a          495 c          727 g         1353 t
ORIGIN      
        1 attgtcgaga tattataata tataactaat gtattataaa taatagttgt aaacttcctg
       61 taacatatat tttaaatgct tattcttaaa tttttgcatt atttattaaa atttattatg
      121 ctttaacgct aaattgagtt tgataattat aaaacaaata atcaaaaata gtgtaaaaat
      181 taatactttt tgttaatatt aatagataaa gataatctaa ggtggtgatt aaactgacaa
      241 tagaagaata caaaaacaaa tactttactg gaaataaaat cgtactttct ataaatagtt
      301 ttgataataa agaactatac tatcatgaaa ctgaaaatat attaaattta ataaatgaaa
      361 tagataaatt aacatcaagt aagatattta aaacaataga agataaaaat gatttggcac
      421 tagagtctta ttattctagt gtaatagaag gtgctttttc aactagaaag attgcaaaat
      481 ctataattag aggaaagatg aaaccaagta ataaaagtga atatatgatt tacaataatc
      541 atagagcctt agaatatgga ttagataact tagatgaatt gtatagtcac aaatttatat
      601 atgatttgca tcatatattg ggcgaaaact gcttggattc agaagaatat gagtatagaa
      661 ctgaaaaagt atatgtatgt gattcaaaag gtgagattat acatacaggg cttgaacctt
      721 tgaagatata tgactttatt agtaaattaa tagattttat ggaaaatagc aaagtaagta
      781 atttaattaa aagtgctata attcattttt actttgtata tgttcatcct tttagtgatg
      841 gtaatggaag aacatcaaga gccttatcat atctatattt gattgataaa ggatatgata
      901 ctttcaagga tttttcaata tcatatatga tatctaaaaa cagaactaag tattataagg
      961 ctatacttga tgtagaaaac aaaggaaata atttaactgt atttatagaa tttatgctca
     1021 agtctataat acaatctatt aatgagatgc gtaatatgca tgatagaaaa tctttagaaa
     1081 gtatattaaa agaagaattg tttgaaaatg acataactct atcagcaaca gaagaacata
     1141 tattaaaata tatttgcaat aaagataact attctatgac attagaaaat tatataaaaa
     1201 agaataaaag tagatactta aaggctggaa taaaggaaat tgaattagtt gaccagttga
     1261 tggaagtgtt taataatctt gaagagatag agattttgtc taaagaaaaa aatatatata
     1321 aagttaatga taagtactta aaaatgcttg atatagacta atttagataa tctggagagg
     1381 gaatatatga ataataagaa aaaggcattt ttgatttctg gggctataaa cgtaatttca
     1441 ttaattattg ttttttcatt gtttcattat aatattatag aaaatagtac acaaaatatg
     1501 ataataactg taatcttaat ttttattttt gaaatgataa aaataaaaat catagagaaa
     1561 tactataaac tataaacaag aaatatcttt tctaagagca tattgtacaa tatgctctta
     1621 atttagttat atagatttaa tataaataat gtattttttg tagatgattt atgtaagatt
     1681 tatgaccaat ttgtgtatga tttgtgttct ttaatcatga caatattata ttgtaaaaaa
     1741 taaatttaat aaaaaatgac ttggaattta tattctaggt cttttttata taaaaattta
     1801 ataagtttca aatgttggta actttgacag tagaaaacgt ctattgagat aacgaggatt
     1861 ctaattgagg tggagagata ttagcactta aagtagtata tgcaacagat ataatcttta
     1921 caaaagtaat ttgaattaaa atgtaaatat aaagaataat taaaaaaata tcgcaattat
     1981 ttgtttttca tatggataaa attggttatt taatatataa ttacaagtat aaataataaa
     2041 atttatttgt aaaaagagta gcacttttaa ttctaaaggc tacttttttt atacaaaacc
     2101 ctatttttta taacaattca ttaacataaa aatcaaactt accttaaaat ataaatttac
     2161 aaatttttca atcaataaga aaatttcttt attccttaca taatgactgc aatgtaacaa
     2221 aataattaca aattaattat gaaatcatta tagttagaaa cctctttaaa attagatatt
     2281 aagtatcctt tagaattaga acgggaacaa agttaaaaaa ttaagtaata tcaaatggac
     2341 aagggggaag aaaataaaat gaaaatacca aagaagattg ctgcaatgct tacagttaca
     2401 atgatagctg gaagttcaac agcaggcata gctagtgcac aaactgtggc aacaaattta
     2461 acagggcaag aaagatatga aactgctgtt aaaataagtc aagatggatg gaaaaatgct
     2521 gatgaggtag taatcgtaaa cgactcatct atagcagatg ctctatctgc aacaccattt
     2581 gcaaaagcaa aaaatgctcc aatactttta acaagtaaag ataaattaaa tgataagacg
     2641 aaagcagaaa tacaaagatt aaaagctaaa aaagtatact taataggtgg aacttctgta
     2701 ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa tatcttttga gagaatctct
     2761 ggagctgaga gatatcaaac atccttagaa ttagcaaaga aattagatgc tataagtgat
     2821 gttaagaaaa tagctgtagt taatggtgaa aaaggacttg cagatgctgt aagtgtagga
     2881 gcaccagctg cacaaaacaa tatgccagta atacttgctg attctaaaaa tggaacagca
     2941 gttgcagaca aatttataaa ggatgctgga ataactcaat cttatgtagt aggtggagag
     3001 agttcaattt ctgaagctgt taaaaataaa ctaccaaata gtacaagact aggtggaaca
     3061 gatagaaatg atacaaatgc aaaagtaata aaagaatttt ataaaaagac agatttaaaa
     3121 aatgcttatg taactaaaga tggtatgaat aaacaagacc aacttataga tgcactagca
     3181 gtaggtgtat taggtgctaa aaatcaatca ccagtagttt tagttggaaa gaacttatca
     3241 gcttcacaaa aatctctagt taactctaag agttttgata agataactaa agttggtggg
     3301 aatggtaatg agacagcatt taatgaaatg aagtcattac aagaagtaaa aacagttgaa
     3361 gctaaaacaa tttctgaatt aaaatcagct atagataaag ctactgctaa tgatgttata
     3421 aactttaagc caactagtga ggttaaagaa gcatttacaa tacaaactga taaagcagta
     3481 acagtaaact taaatggtac atatacaaag actgtgacta tcaacatgcc taatggtgat
     3541 gtaaataact atgcaaaagt ggatgatgta gttatagatg atgtaaaaga tggaactttt
     3601 gttaattatg gaaaaataac taacttaaag gttaatgata aaaatggagc caagatagaa
     3661 aataattcta aaggtgagat tggtagttta acagtagcat ctggagcaag tcaagtaaaa
     3721 gtgactaatg gtggtaagat aactacagtt acaaataatt ctaaagggac tactatagat
     3781 aataaaggta caataagtag tgttaaggga gataattctc caacaataag tggaaatagt
     3841 ccaagttcta actcttcagg aggaagttca tcaagtggag gttcttcaca tggaggaggc
     3901 tcatcttcag gaggaagttc ttctaatcaa acatcagtaa ataatgaagc agcaaaaata
     3961 acaagcgtgc ctacaccagc aaaagatgca actagattaa ctatgccatc agtatcaagt
     4021 ggatatacta tagcaataaa aacatcaagt aatgagagtg ttataaaaaa agatggaaca
     4081 ataatacctc caaatacagc aacaacagta aaattagtat ttacggtaac acatacatca
     4141 agtggtaaaa cagcagatac aaaagaatta tctgtaacag taccagctaa atcaacagat
     4201 gaagaattac aagcagcatt agacaatgaa gtggcaaaaa taacaagcgt tcctgcacca
     4261 gcaaaagatg caactaaatt aactatgcca tcagtatcaa gtggatatac tatagcaata
     4321 aaaacatcaa gcaataagag tgttataaag gaagatggaa caataatacc tccaaataca
     4381 gaggaaacag taacattggt atttacagta acacaagaat caagtggtaa aacagcagat
     4441 acaggagaaa tagatgtagt agtaccagcc aaatcaacag atggggaaat acaagcagaa
     4501 gtacaagctg aggc
//
LOCUS       DAELTA010000223         4504 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.228, whole genome shotgun sequence.
ACCESSION   DAELTA010000223 DAELTA010000000
VERSION     DAELTA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.228"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..496)
                     /locus_tag="KPG13_003406"
     CDS             complement(<1..496)
                     /locus_tag="KPG13_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG0987092.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFN"
     gene            complement(525..2075)
                     /locus_tag="KPG13_003407"
     CDS             complement(525..2075)
                     /locus_tag="KPG13_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG0987093.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            complement(2277..3161)
                     /locus_tag="KPG13_003408"
     CDS             complement(2277..3161)
                     /locus_tag="KPG13_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0987094.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3389..4396
                     /locus_tag="KPG13_003409"
     CDS             3389..4396
                     /locus_tag="KPG13_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG0987095.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
BASE COUNT         1516 a          729 c          510 g         1749 t
ORIGIN      
        1 gattaaatat ccaataatca tttctaaata ataatttatg aaaaataata aatgactttt
       61 caaaatttaa taaaattgga actgttaata ataatggcat aattataagt gttattgatg
      121 tagtttttaa gatttttata tttttatttt ttacactcaa tataattcct actaaagaaa
      181 ctaccaataa taatttagct aatttattta tattttgaaa tatattcctg acttcttcaa
      241 aatgtatctt cccttgtgga gaagatttta atgttggtaa tttgaactca gacacattct
      301 tatttaagtt ataatcaatt agataatcgt agttcaattt tatatcatct ttagataaac
      361 cagataactc tgatatattt aagtaatcaa tgtcaaaata ataaagttgt ttaaatccaa
      421 cagtaaaatt aataacacct actattataa caatactaat acaaattgaa aaaagtacat
      481 ttagaaactt tttcatgaaa tccccccagc aataagttaa atcactacac gattttagtt
      541 gtaaaatctt ctatattcca tacatcatca acaatttctg aataaaattc tggctcatga
      601 catactaaaa gaactgttcc cttaaactct ttgatagcct tcttcaattc atcttttgcc
      661 tcaacatcta aatgatttgt aggctcatct aatactaaga aatttatatt ttttaacatt
      721 actttgcaaa gtctaacctt agcagcttct ccaccactta atactctcat ttgactcgtt
      781 atatgttcat ttgtaagtcc acattttgca agagcctgtc taacttcaaa gtttgtaagc
      841 cctggaaatt cactccaaac ttcatccata ggtgtattac tattttctct tgaactctct
      901 tgttcaaaat atccaacttc caaatagtca cctaatttaa catttccttc aaatggtttt
      961 attattccaa gtaaagtttt taatagggta gattttccaa taccattcat tccttttaag
     1021 gcaatctttt ttcctctttc taaatggaaa ttaagtggtt ttgtcaatgc ttcatcatac
     1081 cctaaaacta aattttctgt ttcaaaaatt attttactag ctgctctagc atctttaaaa
     1141 gcaaaagtag gttttatttt ttcttttggt ctttctaata tttccatctt atcaagttgc
     1201 ttttgcctac tgttagccat tcctctagtt gctactctag ctttatttct agctacaaaa
     1261 tcctctagtc tttttctttc ttcaacttgt ttatcataag cttgttcttc ttgacgtttc
     1321 tttatatcat taagtcttac aaattcatca taatttcctt tatatctatt taactctcca
     1381 ttttccatat gatatattac attacatgta ttatttataa attcaatatc atgagaaact
     1441 aagacaaaac tattctcata ctcttgaagg tacttagtaa gccatgttat atgctcttcg
     1501 tctaaatagt ttgtaggctc atccaatata agaatagttg gattttcaag tagaagttta
     1561 gtaagaagta cttttgttct ttgccctcca cttaagtcag ttacatcttt atcaagacca
     1621 atttctccaa gaccaagacc atttgcaact tcttgtatct ttgcatctat catataaaaa
     1681 ccactatttt caagtatagt ttgtatctct gctgtttctt caagaagctt actcatttca
     1741 tcatcactag cttctcccat tttgtcatac atagcaatca tttcttgctc taagtcaaac
     1801 atatgcttaa aagcctctct aagcacttct cttatagttt ttcccttgct taataccgta
     1861 tgttggtcta agtaacctac tgtagctctt gaagaccatt ttatatttcc agcatctggc
     1921 ataagcttct ttgttattat atttaaaaaa ctcgattttc cttctccatt agcaccaact
     1981 agtgcaatat gttcaccttt tctaagtcta aaggatacat tttctaatat tgttctcgca
     2041 ccaaatccat gactcacatt ttctactact agcatttcaa cacctctcaa aaaaatttta
     2101 cacattctat actataatat attcttttta aacttaaatc aagtattccc aatattttta
     2161 gttatattta cgacaattta acctaaaata aacttcttct ttgtgttatg tacaataaaa
     2221 aatagagata aaaattaggc aaataatacc aacctaactt tttctctatt catatcttat
     2281 ttaaaatacg catccaatcc atcagctata cactgcatca tttttctttg gtagtctgga
     2341 tttgacatca tttggtcttc attatagtta ctcataaatc ccatttctac taatacagct
     2401 ggtacttttg accagttgaa tccagtcaaa tcacctcttt gaacgattcc attgatttgt
     2461 atacctgatt gttccatatt ctgtttgata aattcagcac atttattact ttcttcatat
     2521 ataggagctg tatattttcc atctttttca ggaatcaata tagatgctcc agtttttgaa
     2581 gaattattta aactatctgc atgtattctt acaaccatat ctgctttttt gtcattagca
     2641 agtattgctc tttctgaatt acttatattt acatcatgtg tctctctagt cattattaca
     2701 ttataacctt tagattctaa tatactcttt aaaacaagag atgcttctaa gtttaaaaca
     2761 tattctggct tttttgtagc aataccctct gttcctgaag atactctcgc ttttttagag
     2821 gaagaacctg gagctactgg ttctaaattg ctatctcctt ttccttgatg ccctgggtct
     2881 atacaaatta aaagtttttt atttttttga atttcatttt tattatctga cgatttgtcc
     2941 tctgatgact tatctttatt acttttagaa ttagatttgt cttcattttt atctttatta
     3001 tttttgtttg aattttcatc tttatctgct ttttcatttg tgatatttcc tgaactaatc
     3061 aaatttgtag tcttgtaaaa attgctaact ggatttgatg ttcctataat tatgattccc
     3121 atggcaatta tacaagtaac taacaatcta tattttttca ttttttgctc ctttcttatg
     3181 ctagacagtt attgtttatt tttatttata tgaatatgta tttattttat catatacact
     3241 atttatttta tttattttta tgaaaactaa ggtgttattg agctagtttt ccaagcatca
     3301 aaacatgatt cttgcacaac ataatataga agcagcaaga aaacttcaat tctattatta
     3361 aaataattta ataagaaaga ggtgttttat gcaacttaaa aattttcatt ttaaaaataa
     3421 tactttaagt aaaaaaacca acttagctaa gtcaaaattt acaattgttt taacattttt
     3481 atttttatta tattttttct caaataattt aatttatgca caaaatttac aaaaaaatga
     3541 acctgaatat ttaataccaa ttggaaacgt acttcaaatt gacgcagaat taaaaaatat
     3601 catagttaga aatcctggag aagcatctcc atttagattg ggtgatgcta tagtaaaggt
     3661 aaataatgct gaagttgatg gatatagtga ctttgccaat accttaaatg ctttaccaga
     3721 agaaaaacaa gtatctattt tattaaatag aggtggacaa cttataacaa tgaaaactac
     3781 caaacacata ttagaaaaaa ttagttttaa caatttactt tctggatttg ctactttaac
     3841 gtatatcaat ccagatacat cagagtttgg agctgtaggt catccaataa gtgttggtaa
     3901 tgcaagaaaa atacctatta aaacaggttc aatttctaca actaagcacc ttaatataga
     3961 aaaatcttat aaaggtagtg ttggatgtat aaatgcaaaa cgagaaaata ttattggtag
     4021 ctttacagaa aatactgatt ttggaataag aggtcaaatt aataactttg atatttccaa
     4081 gttaaaaaaa tataaagtag catctttaga tgaagttaaa ttaggaaaag ctcaaataat
     4141 tttacaagat aactttaata agtgtaaaaa atataatatt gaaattatag atatagaaaa
     4201 ccaaaaacat ccagaaagta aaagttttaa aattaaaatt acagataaaa agcttttagc
     4261 tcaaactggt ggtattgtac aaggtatgag tggtactcca atagttcaag ataataaaat
     4321 aatcggagca gtgtcacatg caattgaaaa cgacccagct gtaggttatg gtgtatttat
     4381 taaatggatg ttgtaaataa tataaataaa taagttttaa gatgttatta agttattaat
     4441 aatatatttg gtctaaaggt acttttattt aacctacaga ccaaatatat tagatatttt
     4501 acat
//
LOCUS       DAELTA010000224         4419 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.229, whole genome shotgun sequence.
ACCESSION   DAELTA010000224 DAELTA010000000
VERSION     DAELTA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.229"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            124..1290
                     /locus_tag="KPG13_003410"
     CDS             124..1290
                     /locus_tag="KPG13_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG0987096.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            1312..2958
                     /locus_tag="KPG13_003411"
     CDS             1312..2958
                     /locus_tag="KPG13_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG0987097.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            3055..4419
                     /locus_tag="KPG13_003412"
                     /pseudo
     CDS             3055..4419
                     /locus_tag="KPG13_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1640 a          498 c          862 g         1419 t
ORIGIN      
        1 acatacttaa gaaaacggat aaaatcaaat ataataactg tatattaaaa taacttaaaa
       61 aaattatttt attataagtg caaaatatac ctatttaaat aagataagag gggaggattt
      121 tatgtgtata taaaaggtgt aaatttagga ggttggttag ttttagaaaa atggatgacc
      181 tcaagtttat ttgaaggcac agaagctgaa gatgaatact atttaccaag acagctttca
      241 agagaggcat atgaatctag aattaagact catcgtagtg aatacatcac agagagagat
      301 tttgcaatga ttaaatctat gggatttaat tcagttcgta taccagtacc atactttatt
      361 tttggagatt gtgaaccatt cataggatgt gttaaagaac ttgataaagc atttgcttgg
      421 gcagataagt atgggcttag tatattgata gacttacata cagttccagg aagccaaaat
      481 ggatttgata atggaggaat atcaggtata tgtagctggt cacaaaatcc agaatatgta
      541 gcgtttacat taaatgtatt agaaagactt gcaaaacgtt atgggatgag gcatgaattg
      601 tatggaatcc aaattttaaa tgagcctata acagaaagaa tgtggaacat aatgaatgtt
      661 ccaaatcggt ttaaggctgt agataaggag atggcaaggg ggagtaaacc taattcatta
      721 gagtttttac gtgattttta tataaaagca tataaggtaa tgcgtcctta tatgagggag
      781 gaaaacgtta tagtatttca tgatgcattt gaattaaaag catggaaaga ctttatgaga
      841 gaggaagaat ttaaaaatgt tgttctagat acacaccaat atttgatgtt agctgaagca
      901 gatggatgtg aacaaagtat tgatagctat ctcaaatata ttagggaaaa ttatgctaaa
      961 gatattttac aaatgcaaaa atattttcct gttatatgtg gggagtggag cttatttaat
     1021 tcatatgctt gtggtataga tacaaatgga ggtcaatcac ctctaaatgg gattgaatca
     1081 aatatagata aattatcaaa agacgataaa agagaattat ataggaagat agcaaaagca
     1141 cagcttgatg catggagaaa tggaagtggt cactactatt ggaattataa gttattacta
     1201 gatacagtta atgaagaagg ttggatagga tgggatagtt gggacctagt caaatgtgta
     1261 gctcaagaat ggtatcctat agaatattaa actaaaattg gaggaaaaga tatgattaaa
     1321 aaccctatac ttccaggttt taatccagat ccttgtattt gtagaaaggg agatgattat
     1381 tatcttgtag tttcatcttt tgaatggttt ccaggtatac ctgtttatca ctctaaagac
     1441 ttaaaaaatt gggagttata tactcatatt ttgacagatg aaacaaaaat agatttgaag
     1501 aagcttcctt catctaaagg aatttgggca ccctgtttga cttattgtga agaagaagat
     1561 ttattttata tagtttatgg aattatgaac tctatgaatg caagatattt tgatgttgat
     1621 aattatttaa ttacatcaaa agatataaag ggagagtgga gtgaaccagt atatctacac
     1681 tcatcaggtt ttgatgcgtc tatttttcat gatgatgatg gtaaaaagtg gattgcatca
     1741 ttagactggg agacaagaga aggatatgaa aaacctggtg ttatatgttt agtggaatac
     1801 tgtacaaaaa agaaagaaat agtaggatat ccaaagagaa tatggtcagg gggaactgat
     1861 agaggttgta ttgaagcacc acatataaca aagcgtggag attactatta tataatgtgt
     1921 gcagaaggtg gaactggata tggtcatagt gtaactatgg gaagggctaa aaatatatgg
     1981 ggaccttatg aaaaagattc aatgaatcca attgtaactt caattccagg cgatttttat
     2041 gaaagacatg accctgacca tctaaagcct aaatactata atccagaatc aaaacttcaa
     2101 aaatctggtc atggaagcta tatagaaaca acatcaggtg aagtatattt agttcatcta
     2161 acatctagac catttgtacc agagttaagg tgtactttag gtagagaaac agctattcaa
     2221 aaaatgaaat ggacaaagga taattggctt cgaatggaag atgaaagtaa tttggcaaaa
     2281 gaatacgtat cagaaagtaa actagaagaa catttagttt catctatacc aagttttgat
     2341 gactttgatt caaatgaatt aggtttacag tactatgctc ctaggatttc accattatca
     2401 tttgcagatg ttaagtctag accaggatat gtaaggatta gaggacaaga atcaagaaca
     2461 tcattaaata aagtgagcat attggcaaga aaattaactt cagtatatgc aagaataacc
     2521 actaagatgg agttttaccc agaggtacat cagcatagtg cgggtttgat aatgtattat
     2581 gataatatga attatataaa tttaagaaag tattatagtg aaacattggg tcaaagtgca
     2641 ctatcaataa tacatcttga aaatggagag aaaacggaat ttttaaatac taggatacca
     2701 ataaaagata taccaatata tcttcgtctg tatgtacaag gtcgtaagtc atattttgaa
     2761 tggagttatg atgaaaaaaa ctaccagaga ataggaaagg tatttgatac aactaagttt
     2821 tcagatgagt actgtaaata tggagaattt acaggtacgt ttataggatt aacttgtgca
     2881 gatcgtgtaa agcataaaca ctatgctgat tttgattttt ttgaatatat agtagatgaa
     2941 tcaaaagatg tcgattaata aaatcattac taaaattttc agagcaaagg taaatcttat
     3001 gctctgaaat aagaaacatt agaaggcaaa tcttacaaaa aggagtatgg tattatgaat
     3061 acagaagtta atgttaaagc aactacgatt agatatgaaa agatttcatt ttttgaaaga
     3121 atttcttatg gttgtgggga tttggggtgc aatatcatat actcagctat gtcagcattt
     3181 ttgctgtttt attatactaa ttatgcagat gttagtgcag cagctgtagg tagtattatg
     3241 cttgtatcta ggatactgga tgggtttagt gacttaacaa tgggaattat agtggataga
     3301 actaaatcca aatacggtaa ggctaggcct tggattttga gaatggcgat tccttttgct
     3361 atagcagcag tattattatt ttctgttcca tcaaatcttg gtatcacttc taaattaatt
     3421 tatattttta ttacatataa cttagtttct actgttattt atacagcaat caatgtaccc
     3481 tatgcaactc taaattcact tataactcaa aaccagtatg aaagaggagt attaagtata
     3541 tttagaatga tattagccac ttgtggtacc ttactaataa atggacttac actacctcta
     3601 gttgaatact ttggaaataa tttatcagca tggactaaaa cattctttgt atttggaata
     3661 gcatctataa tggtattttt tataacattt actggaacca aggaaagagt aaaagcagtt
     3721 aagcaagata ggaatgaagt aataccattt aaaataggta ttaaatcttt atttagaaat
     3781 aagtactgga tacaaataac attatgttta gtttgcatat ttattgtatt tgcgataaat
     3841 ggtggttcat ccgtatatta tgcaaagttt attttgggag atgaaaaatt atttggtcct
     3901 ataaatatgg tttcaaatat atcccaaatt atagctatgt ttatggttgc tccttttata
     3961 aaaaaatttg gaaaaagaaa tgtattaata gtaggttaaa taatattaat atcgtcaaat
     4021 gttatgttta taatagttgg acaaaatcat ataggagtaa tatttgcaag tgctataaag
     4081 ggaataggaa gtgctggaat agcatcaaca atgtttgcta ttgtttcaga tactattgaa
     4141 tatggagaat ggaagactgg atatagaact gaggggctta taaatagtgc atctagtttt
     4201 gggtttaaag tgggaaatgg attaggttca gcaatattag gtgcagtatt atcaattggt
     4261 ggttatgtag gaacatcagc tactcaaagt gatttagcta tagtatcaat aaaagtatgt
     4321 tttatatacc tacctatatt tataacaata ttacaaatta tcataatgtc tttttataag
     4381 cttgataaag aatatagtac tatacttaat gaattaaat
//
LOCUS       DAELTA010000225         4404 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.230, whole genome shotgun sequence.
ACCESSION   DAELTA010000225 DAELTA010000000
VERSION     DAELTA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.230"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            595..1074
                     /locus_tag="KPG13_003413"
     CDS             595..1074
                     /locus_tag="KPG13_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0987098.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            1071..1850
                     /locus_tag="KPG13_003414"
     CDS             1071..1850
                     /locus_tag="KPG13_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987099.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(2100..2600)
                     /locus_tag="KPG13_003415"
     CDS             complement(2100..2600)
                     /locus_tag="KPG13_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0987100.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3212..3919)
                     /locus_tag="KPG13_003416"
     CDS             complement(3212..3919)
                     /locus_tag="KPG13_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG0987101.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1615 a          647 c          465 g         1677 t
ORIGIN      
        1 taacctgtaa ttaacttata aggtctttta ttattttgac atttcaatat aaaaatttta
       61 atcaaaaaac tttattattt taaaaaaaat tattattttt ataatttata ttttttaagt
      121 ttatttatgt tcatctctat ctaaaataat gacatttcta actcttaatt ctcatttaag
      181 tttcattgtt ttttcttaaa agcctacttt tagtcaagat actgttatct ttactcatta
      241 tttaaagaac ttattttaaa tctgtacatg aagtaaataa tccaaaagca ttgttattct
      301 aactactcaa cattaaatac ttactatttt ctaattactc aatataaaga cttactataa
      361 aactaaatac ataaatggct tctaaagttt aatatatcta catggcttta tatatgacag
      421 gaaaaatact agaaaaaata tattatagaa tacttgtata taataggcaa aactttcaaa
      481 cttaaattta tcaaataatc ataaaaaaag tgaaccatct atagtaaaat tttatctaat
      541 aagtgaaagg caaaaaagta gcgctacgat ttgcatagaa gggaaggaac ttagttggat
      601 aaaactacat ttacaaacaa tatacttgaa tcagaacaga ctttgtatcg agtatctaag
      661 tctattctag gaaatgacca agactgtgaa gatgccgtca acaatgctat tttaaaagca
      721 tatgaaaagt tggattctct taaggaagaa caatacttta aaacatggct gattagaata
      781 gtcataaatg aatgtaattc attaagacgc aaacgattaa aatctctatc atttgaagat
      841 gtcttcaaaa acaaaaaaat tgatgaaaaa gatgactata gcgatttgta tactgctatt
      901 caaagtctac ctaaaaaaat taaaattcca atagtccttt actatattga aggttattca
      961 gtagatgaag ttaaggaaat attagatata cctcaaggaa ctgtaaaaag tagattatca
     1021 agaggaagaa gattattaaa aactaaatta gaaaacacag aggtaatcat atgaataaaa
     1081 atatattaaa taattcgttt ccagaagttc cagaaagttt tcataatagt ttaagcaaaa
     1141 cactaaatag tttaccagaa agagaggaaa attataaaat gattaataat aaaacacaca
     1201 aattaccatt tagaaaaggc ttagttgcaa ctttagccat tactttgata ttgagcacaa
     1261 cagcccttgc agtaggacat atatatagtt tagttggaaa atcaagtact aaggctacct
     1321 atacatctat cccaactaca gaacaagtaa aaaaagactt taactttacc cctaatatag
     1381 tttctgaatt tagtaatgga tataaattta aaggagcata tactgtaaat aaaaaagcat
     1441 ttgacaaaga aaataatgtt ttaggaaaat caaaagcact tagttttgaa tataaaaatg
     1501 gcaatgatga aatatctctt gacacagcaa ataatgtact tgggaagagt tctgacaatc
     1561 taaaagttgt agatacttac aaagatatta atatttctta tgaagcattc gcacaaaagt
     1621 ttataccaga aggttataaa atgacagaac aggataaaat tgatgaaaaa tcaggcaaat
     1681 acacatttag tgtggggcca aataatgaat cagacaaagt tgaagttaat gaatttaagt
     1741 ttttgacatg gaaacaagat ggagtttact attcttttac agttcaagac tcaaatttat
     1801 ctatgaatga cttagttaaa atggctcatg aagtaataga tgcaaaataa acataagtaa
     1861 taataagttg tttatgagtt tattatttat gactttaatt tttttattag taaacatggt
     1921 tgtatcttcc atctttatat tttctttaaa tttaatatat aaaattttag atatttagct
     1981 accttcaaaa gaagcattat taaaaagccc ttaaaaatat ttcttttata tggaatttta
     2041 agggctttaa ctttatttta tacaagtgta tatggaattt taaagacttt aactttattt
     2101 tatataagtg taagctccat ttgaattact tcttctttta caggtctatc attccatgta
     2161 aaaccactag atattgtatt tacctctaca aaccctaaag atttataaac atgaatagct
     2221 ctactattac ttttaaatac atcaagctca attttttcat acccatattc ttttgataaa
     2281 tcaataattt gttttaaaag actacttccg ataccttttt ccctatactc tttaagaata
     2341 cccatgccta aataaccaac tttttcagtt ttaggtaaaa cgtcacacca accaatacag
     2401 ttatctgatt ctaaatcaat tataaacagt tgtggtaagt ttttatttat aatatttttt
     2461 ataaattcaa ctgtttcttc aaaaggaaag gcttcatcca tagctagata tttcctctct
     2521 tttgctattt tatcaaaagt ttgccaatac gaataaatat acttttcttc tgcctttata
     2581 aattttatgc cttctatcaa cttaacattt ccccttttta atttttgatt gtagttaact
     2641 tattttatat gattctagtt tataatgcta tataaattac tatatattag cttaaaatat
     2701 ctttgtatat ttctataaaa ttaaaaccaa tatatctatc tcttgcatct ctattaaaat
     2761 aatttttaat agtataataa atatagttct ttaatctttt gggtatatat acttaatcat
     2821 tttgaaattt gatttaaagt ataataaaat atataaaaaa atatataaaa tggtactata
     2881 aaatttttaa tcttcatcaa tacctttttt gctttcattg ttaaaactat tttttctatt
     2941 agtttatttc ttataaaaca aacatttagt attaaaatat atatcacaca atagtatttg
     3001 caatttaact ttaaaaaaat aaaaataccc aactgaccta agcaactcga ttgggtataa
     3061 ataatctaaa aaatcatttt tgtataaact ctttatttaa cttacatttt tatttacctt
     3121 ctactttaat tcaacttata tttctatgct tcttatactc tcattcaact tatatttcta
     3181 cttatcttat acttttataa attcatcaca attatttagt tctcgctatc aaagaaattg
     3241 tattaaaata ctttccattc tctacttcca tcattttttt gtcatttata gcatatgaat
     3301 tatcacaatt tatccaatcc ttccaagctt catcatgaca atttaaagct tcacatttct
     3361 ctacaatcac agactccgat tcagaccaaa gtttattcca ccatgtaatt gaatggaagt
     3421 tcatattatc ttgccaaaat ggaatgagtt catctggtat acaatccacg aaatcttctt
     3481 ttaatccagg cattgccata gcaagtattc ccccctcttt tacaagaggt gatatatgat
     3541 tgtctaaaaa tccttctttg tttccaaaat agtgataaga atcgatactt atcaccgcat
     3601 caaagaaacc ttctgcataa ggtagttcat gtgcctctgc ctgtattgga aatattttat
     3661 catccaattt aaactcttta aatcttttat agttttcagt tggttctatc cataaatctg
     3721 ttgcaaatac agtagcatca aattcctttg ctaaaaatat cgaagatata cccttaccac
     3781 aacctaagtc taaaatacgc attccctttt ctagttttat cttacttgtt aattcttcca
     3841 atatttttat acaattaggt cccatcatat ttttcatgag aaagtcctta tcgtatttat
     3901 tactttttgt aaatatcatt ctatctttcc tttccctatc agttattatt tattaaattt
     3961 tatacgtttg attttttatt ttttatctgc ttcttatatt gagcaatctc ttttttattc
     4021 attctttaac agacaatttt aatgtgtaat tttatccact ctgtgatagt aactttaatg
     4081 aataatctta tctactgtga tagttatttt agtgagtaat tttatctact ccacagcaat
     4141 cgctttaatg agtaatctta tccactctat gatagttatt ttagtgagta attttatcta
     4201 ctccacagta atcgctttaa cgagtaatct tatctactct ataatagtca ctttaatgag
     4261 taatcataat ttttatatca ttttagatac ttactctaat aagaattcat tttctaatac
     4321 ttaataagtt ttaattactt ccactttaac ctatatacta attaacttat ttaatttagt
     4381 gcatatttga cactttacct ttta
//
LOCUS       DAELTA010000226         4388 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.231, whole genome shotgun sequence.
ACCESSION   DAELTA010000226 DAELTA010000000
VERSION     DAELTA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.231"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            231..1310
                     /locus_tag="KPG13_003417"
     CDS             231..1310
                     /locus_tag="KPG13_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0987102.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KPG13_003418"
     CDS             1634..2002
                     /locus_tag="KPG13_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG0987103.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="KPG13_003419"
     CDS             2119..2922
                     /locus_tag="KPG13_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0987104.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="KPG13_003420"
     CDS             3314..4204
                     /locus_tag="KPG13_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG0987105.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1674 a          482 c          826 g         1406 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaata
//
LOCUS       DAELTA010000227         4132 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.232, whole genome shotgun sequence.
ACCESSION   DAELTA010000227 DAELTA010000000
VERSION     DAELTA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.232"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            292..1104
                     /locus_tag="KPG13_003421"
     CDS             292..1104
                     /locus_tag="KPG13_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG0987106.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1193..1384)
                     /locus_tag="KPG13_003422"
     CDS             complement(1193..1384)
                     /locus_tag="KPG13_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987107.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1677..1895
                     /locus_tag="KPG13_003423"
     CDS             1677..1895
                     /locus_tag="KPG13_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0987108.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2204..3823
                     /locus_tag="KPG13_003424"
     CDS             2204..3823
                     /locus_tag="KPG13_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG0987109.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT         1546 a          485 c          574 g         1527 t
ORIGIN      
        1 aaatataaac ctaatttata gttaaactta taaaataacc cgttatgtat aacatattaa
       61 aatattttct atatatttat atgactaagt tctataacta aattgaaata atcacttttt
      121 caagaatgtt ataatttttt tattgtattc tttttgctat agtctatgaa taaatcaata
      181 gaaaatcttc taagttgctt ttaaataatg actttatggt tcaattgtac taaaatagta
      241 tatgtaggtt acaaataatc taataaatat taaagtttgg aggtaataga tatggcagat
      301 tgtaattcat gtccatcaaa gggaaattgt aatagtcaat caaattgttc tattgaaaat
      361 aaccctaaca ataaatttgg aaaaataatt ggtgttatga gtgggaaggg tggagttgga
      421 aaatcaactg taactgcttt acttgctaat aagctaaaca aaatgggata taaggttggt
      481 atattagact cagatataac tggaccaagt attcctagac ttatgggtgt aaaaaatgta
      541 aaagcttatt ctgatggctc ttatatttac cctgtagaaa actcaaataa cataaaagta
      601 atgtctataa accttatgat agatgatgaa aatgaacctg ttgtttggag aggaccttta
      661 cttggagggg ttgtaaagca attttatact gatgttcttt gggaagaatt agactattta
      721 ttaatagata tgcctcctgg tactggagat gttgccttaa cagttatgca atctattcct
      781 ataagtggaa tagttatggt ttcagtacct caagatttgg tatctatgat tgtatcaaaa
      841 gctgttaata tggctaaaaa aatgaacata aatgtccttg gagttattga aaatatgagt
      901 tatatccaat gtcctgattg ttctaaaaaa atcaagttat ttgaaggaga atctactgaa
      961 aagtttttag atgatttaga cttagagctt ttaggagaac ttcctatgac taaagaaatt
     1021 atagatatta ctcataatgg agttacagaa attagtgatg atttagattc aatattgact
     1081 aatgttgttg aaaaaattaa ataaataaaa attttataat tttatattta tcaaaaaaag
     1141 aggcttgtat ttattttata atcatacaaa taaatataaa cctcttttat gtttatctat
     1201 gttttaaata cacaaaatat tcttctactt catctccatt ttttactttt actaaatatt
     1261 cattataatg agcattatat ggctgtgtca ttttgcattc tattacattt tctatgtctt
     1321 ctaattctaa aagttctaca tcctttaaaa attttctttc tatgagttcc attaatcttt
     1381 ccataatata tcctcacttc ccctataatt aagataaact atatatcctc ttaatacaga
     1441 gtttatctgt ttattatatt atatccaata tattacagtt tctaacatca ttcataaatt
     1501 attttaacaa atagagattg attttttaat aaatatgcct tgataaaatg aaacatagac
     1561 caactcttag ttttatgtaa agaaaactta aagaaaacta tagattatat tcacaaaaat
     1621 tactcagaag atataacagt aagtaatata tgtaatgaat tacaacttaa taaaagttgt
     1681 ttttttcttt atttaagaaa aaaagtggat atacttttac taatttttta agtaaagtta
     1741 gagttgaaaa aagcaaaaag tatatattaa aaaaagactt gtctatttta gacgttgctg
     1801 tactaattgg attcaatagt caaaattact atagtataat ttttaaaaag tttaataact
     1861 ttacaccaat tgaatataaa aatatttata actaaaaaat atcattctaa aaggatgtta
     1921 tttttttaca tattttttac aattatagtt aattctttgt taaattgatt gtacataaaa
     1981 tactaaaaat ctaagttcaa gtatttaaat tgctatatat cataaatttt ctaaaattaa
     2041 caacaactta aaaatttaac attctcttaa cgtatactta acaaaacatt aatattttta
     2101 ttgacaaaat ttgttattct atattatgga aacaataaat tttagttaat aaagtttata
     2161 attcagataa aaaaattatt aatttcttaa gggaggaaat atcgtgaata tagtaattag
     2221 ccttatcggt ggacttggac tttttcttta cggaatgagt ttaatgggag aaggtttaca
     2281 aaaatcagca ggagacaaat taaaaaaaat tattgaactc ttaacaagta atgttgttat
     2341 gggtgtactg gtaggtacag tcgttacagg tattatacaa agttcaagtg ctactactgt
     2401 tatggttgta ggatttgtaa atgcaggtat aatgaatcta agtcaagcta ttggtgtaat
     2461 aatgggggca aatataggta ctacagttac tgctcaatta gtttcattta atcttgaagg
     2521 aattgcacca attgctttag gtataggtat tttattctat ttatttacat caaatcaaaa
     2581 aacaaagcat ttatctgaga tacttatagg tttcggtata ttatttacag gaatggagtt
     2641 tatgaaagat gcagtagctc cacttgctga atataaagca ttcactgatg cacttttata
     2701 ttttagtaaa aatcctgtat taggtatatt ggcaggattt gccataactg gtattgttca
     2761 aagttcaagt gcatctatgg gtatgttaat agcactagct tctcaaggaa tacttcctct
     2821 atcatctgct ttacctattc tttatggaga taatataggt acttgtgtta cttcactttt
     2881 atcaagtgtt ggtgcaagta gaaatgctcg acgtgcagct gtaatgcatt taagttttaa
     2941 tgtaattggt acaataatat ttatgcttgt tttaaataaa cctatatcag ccatagttac
     3001 tcattttgac ccaactgata cagctagaca aatagcaaat gcacatactt tatttaatct
     3061 tacaaatgtt ataatattat taccattttc taaatacata gttaaattag caaatagact
     3121 tatacctatt aaagaaactg aatcagaaat agtaaataat actaagtact tagatgaaag
     3181 gatgtttagc actccatcta tagcacttgg taatactgta caagaagttg taagaatggg
     3241 tcataaagct acaaactctt tggaacattc aattgcagga tttttaaata agagcaatga
     3301 agatattaat aaagcttttg agtcagaaaa agttgtcaat aagttacaga aagatatatt
     3361 gaactatttg ttaaaacttt ctaaagaacc tcttagagat gatgaacgct ttagaacaga
     3421 tttgcttttt aatactgtaa atgacattga aagagtctct gaccatgcag aaaatatagc
     3481 tgaattggcc atgagtgtta aggaaatgaa cattagtttt tctgatagtg cgataagaga
     3541 aatatacgaa atatacaata agacaattac taattttaag gatgccttaa tagttttaga
     3601 tgttaaagat tttgagttag caaataaagt tttagaagtt gaaaatgaag taaattattt
     3661 agaaaaaact tttagaaatt cacatatgat tagactaaat aatggaagtt gtacaattga
     3721 tgctggtgta ctttatcttg acttacttac taatctagaa agaatttctg accactcaac
     3781 aaatatagta aaacaagtac taaaacttaa acaaaaaata tagtgattat ttttatatat
     3841 aatcttaatc aatgagaagt aagaagctat agataatgct tatatcattt atggcttctt
     3901 attttttatg taatactcaa atttttatag tattctctta ttattaaaca gtttctgcct
     3961 agaatattcc atatatatat gacttctttc ataaaacttg aactgcccat aaataaagtc
     4021 acaagtatac ataactattt tttaatcaat tataaccaat atttgtttta tttatcttca
     4081 ttttaattct tcattcaata atgtaataat attatttttt atttggatac ta
//
LOCUS       DAELTA010000228         4043 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.233, whole genome shotgun sequence.
ACCESSION   DAELTA010000228 DAELTA010000000
VERSION     DAELTA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.233"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1139..1891
                     /locus_tag="KPG13_003425"
     CDS             1139..1891
                     /locus_tag="KPG13_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987110.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1881..3992
                     /locus_tag="KPG13_003426"
     CDS             1881..3992
                     /locus_tag="KPG13_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987111.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1488 a          576 c          509 g         1470 t
ORIGIN      
        1 tatattacag agtcctattt attataggat ttttattata ttgtaaaatc ttatttatta
       61 taggattttg ttatattaca gacttttatt atattgaaat ctcttattta ttgcagtgtt
      121 tctattatat tgcaaactct tatttattat ggtgttttgt tattgcaaca tttctattat
      181 attgtaaagt cttatttatt acagttcttt attatactac agacctttat tattacagca
      241 tttctattac attgtaaagt cttatttatt acagttcttt attacactac agacttttat
      301 tattacaatc tcttatttat tacagttctt tgctatacta cagaccttta ttattacagc
      361 atttctatta cattgcaaag tcttatttat tacagttctt tattatacta cagactttta
      421 ttattacagc atttctatta cattgcaaag tcttatttat tacagttctt tattatacta
      481 caaaccttta ttattacaac attttattat tttaacttct ttactataat atagtaatat
      541 tttaatatct caaaatcata tcaatcactt ttcagcatag tctaaaatac ttccactttc
      601 taaaattatc tatcatacct atactaataa tactttttaa cctttttatt atacatttca
      661 attgtttcta acaacctatt tatttccaaa actccaatat attttctcaa ttacattgtg
      721 ttaatagccc ctaaatcctt tcaaattatt tacaatacat tccattaaat aatcttactg
      781 ctctataatt ctttttttct ttttaaccct actatacatt atttacatca tttttttcaa
      841 tacaacattc gttattctat aaccttttta cttttactat atacttcttt tctaattaca
      901 ccttcaatgt tccacatcat attactttag cacaacaatt aatacctaaa aataaccctc
      961 taaatcctcc aataatataa tcaatttaac tataataata acctctcaca ctatccaaac
     1021 aatatatttt caaacattac aaaaaaacat ccaactatta caatatagct catatccctc
     1081 ctcccctttc aaatgctatc attgacttac aagataaatg aaagtgagga tgatatatat
     1141 gcttaaagta aaccaattat gtaaatcata taaaacaggc agtaaaacat atcctgtatt
     1201 aaaaaatatt tcttttgaaa tcaacaaagg agaatttgtc gcaatcatgg gttcatcagg
     1261 aagtggaaaa actactcttt taaattgtat ttcatgtttt atcccaattg ataatgggga
     1321 aataattctt ggaaataata atataagaaa tttaaaagaa aaggaacttt cagaagttag
     1381 aaatcaacaa ctaggatttg tattccaaga cttcatgcta tttgatggtc ttagtgtctt
     1441 tgaaaatata tgtgttccac aaattatcaa aggtagtcaa gtcttacaaa tggaaaaccg
     1501 tgcaattaat ctatgtgagc tatttggtat tgaacatatc agaaaaaaat accctgccga
     1561 aatatctggt ggagaaaaac agcgtacagc agtagcaaga gcactcatga acaacccaga
     1621 tttaatatta gcagatgaac caactggtaa ccttgatagt aagtcttgtc gtacagtcat
     1681 tgaatcattt ttaaaagcaa agcaagaatt agatgctact atactcatgg ttacacacga
     1741 tagctttgca gcaagcttct gtgaccgagt tattgtgctt aaagatggaa taatttataa
     1801 agaagttgta aaatcaaatt caagacataa attcctagat gaattactag aagttttaaa
     1861 agaaatggga ggcgctcaag atgacaatta ataaattata cagtaaactt cgagcctata
     1921 attggaagaa ctatacaact ttattctttt gtataatact ttctgtggta ttagtaactt
     1981 catatgctct aatttatttt agcccaacaa tacaaaacat acttccaact ggtggagata
     2041 gcagaaaaca ggctacaatg atattctcag ttgctatatt aggatgcgct gtctttacta
     2101 catacgcttc ttccctcttt ttaaaattta aaagcaagga aattggtatt ttcatggctc
     2161 taggaacaga taagaagaaa ttaaaaaaac ttctattttc agagatattg ttaatttcaa
     2221 tagtatgctg ttttttaggt cttatattaa gtattcctgt atcttttggt atatggaaaa
     2281 tatttcaatt agtgattatt gataccagag aaatgacata tcattttgga actcaaggat
     2341 tcatatatgg atttatattt agtgtatttg tgacattatg catatttata ctttctgtaa
     2401 aatttattaa tcgtacaaat ataattgata tattaaatga ccagagaata tctgaaccaa
     2461 tacaggatgt aaaggactgg tatggtatat taggtataat ttttattata ctcggtattt
     2521 ttctaggata tctcgtacct tcactaacta taaacaaatt aaattataaa ctaccatcta
     2581 tatggagctt tacttatcta ctctctctta ttggaatata tatgattatg acatacatag
     2641 ttgtacatgc taaaagagga aaacatccag agaaatacta taaaaacatc atatcaacta
     2701 gcatgatgag atttatggga agacaaacag taaaaaacat gtgtgttatt tcatttttga
     2761 ttgcaggagc attatttgct gtattctatg ttcctaatat ggttacaggt atctatgata
     2821 ctcttaaaaa taaccctata gattatactt ttacctacaa tcaaagagat aaacaggtat
     2881 caaaaaaaga tattgaacaa ttagcagata aacacaatat aaaaattcaa aactatatgg
     2941 aaattccttc tatagaatta attgttgatg gtgtagaatc aatatatcat aaagatggaa
     3001 ctatcgaaaa ggtatatatg aaaaaaagct cctatgatat gttctttaat tccaaagatt
     3061 tttccaagct aattggcaaa ccaataacta taaaaaatgg tgaatatctt gctgttgtaa
     3121 ataaaggaaa tgaaaatcaa gattataaat ataccaaatt tatatcaagc ccagtcacag
     3181 aaaaagaaat cccctttaga tatgctggaa aaataatatt taatggttct ttttttcaaa
     3241 atagaagtat gaacgcctat gtccttaatg aggaggatta taaatcattt actaaagatt
     3301 tatccataaa aaattacaat aattctatac tatttaatgt agataaagat acctatgctt
     3361 ttgccaatga cttaaagaaa gaaattataa aaagaacatc taaggaaatg ggcatgttca
     3421 gtggttatga tgaatacgta aaaaaacgtt atgaatcaac tggaagaaaa tattttatgg
     3481 atgaagaata tccaagtggt gaaggtcatt taaaactaga cccaaacaac aatcagttgt
     3541 atcttaattg gaaatactat cctaacttta aaattttaca aagccaagat atgataaaaa
     3601 acatggcagt cttcttaatg ttattcatat atgtggcaat catatgtttt acagcagtag
     3661 gaatcattgc atatacaagg ggtgttacta tagctataaa tcacaagcag gtctttatgg
     3721 atttaaatcg attaggagca aacaatgatt atataaagtt ctgtattaga aatcaattaa
     3781 agaaagtatt ttcataccct cttattgcag gaagtttttt aatttatctg tttaatttta
     3841 tgattatgtt aaataatgat ggtagaatcg tcaatagtga ggtcaatgct cttttgataa
     3901 acttaggttt tatagggatt tgtggggttt atatgtacct tatatatcta ttaacttata
     3961 agaaattcaa aaaaattatt ggtattttat aataaaaaag gttattagat ttttaaagta
     4021 ataaaatcat tggtgtttta taa
//
LOCUS       DAELTA010000229         3986 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.234, whole genome shotgun sequence.
ACCESSION   DAELTA010000229 DAELTA010000000
VERSION     DAELTA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.234"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            23..379
                     /locus_tag="KPG13_003427"
                     /pseudo
     CDS             23..379
                     /locus_tag="KPG13_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            689..1006
                     /locus_tag="KPG13_003428"
     CDS             689..1006
                     /locus_tag="KPG13_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987112.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(1991..2206)
                     /locus_tag="KPG13_003429"
     CDS             complement(1991..2206)
                     /locus_tag="KPG13_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987113.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(2389..2733)
                     /locus_tag="KPG13_003430"
     CDS             complement(2389..2733)
                     /locus_tag="KPG13_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987114.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            2860..3243
                     /locus_tag="KPG13_003431"
     CDS             2860..3243
                     /locus_tag="KPG13_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987115.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT         1569 a          450 c          477 g         1490 t
ORIGIN      
        1 tttttaataa ttatattata caataacaat atataagcta attaaaaacc ttaagaagaa
       61 cgatattgac actacttgaa ttatattata gaaaagctga aataaataaa atagtttggg
      121 ctagaatagg ttttaattta tatctttcta aaggagttgc attaacttta actgatgctg
      181 gtatagggat ttcaggactt ttggctggtg ctattcctgg tataggacag tacttgccac
      241 ttatagttag attaattaca atgtaccttg agtcaaatat aacatctgga aaaatcttta
      301 aatttaaaaa atacgttata ataaaggagg tgcttgtgta accaaatact acttacaatc
      361 tgtagaaatc aataggtgat aaaatgatta taaaatatct actttttgca attgtttcag
      421 cattaatttt attattttgt aataaagcat tccaactaag taaattacac aagttcatta
      481 tattgtttgt ttcatttttt atactaactt ttgtagcatc attcattagt agataaaaaa
      541 taagatggtg gggttaactt accatctttt tctcatttaa tatttgtaat attttagata
      601 ctctttatac ttgtgttact gttatacttt gattaggaaa attaatacaa catgagttga
      661 tgattatatt ataaaggagg tatcttctat gaattttagg aacatttatc ttatacttat
      721 gggattggga attataatat taactacaat aatatcttta attgaaatta aaattggttt
      781 ttgtaatgaa aaatatttta ataaattaga atcaaaatat ggaaatatag atagaaaaag
      841 aactattaaa ctagaagtac tttatcgata tgtaacaggt tttgaatata tagcaatagg
      901 attatttaca aaaagattag atattacaat aatagcaata atattagtag ctactataac
      961 agtaatatta tattacttag ttagaaagag atatataact atataaaatc taatagaatt
     1021 aatatcaaaa gaaggtagca actaatcacc agcaattaaa ataatttgtt tttcaaagtt
     1081 aataaaagtc ttctttatag caatgattta aatataatca aatattgtaa cggagatttt
     1141 tttatgaata atttaagaaa acaattaatc aaagaaatta tataagatgg agaacttata
     1201 aaatctacag atataagtag ttttaggagt aaacacagat gacttatttc aaagagacaa
     1261 tagctacaag ttatcctcat tctgtaatac aaaaatgttt aatacattag ataagaaata
     1321 ctttaaatat gtttcataca aggactcaaa agaattaata agtaatttta aatctgtata
     1381 taggtcagtt aatgaataag aagccttatc gactttagat aaaatagatt aaaaataatg
     1441 taaacagtat acttcagaaa ttaaaatttt gggaaataac tggaatgttg tagcatcatc
     1501 ctttagtttt actaaaataa gtaatatcta taatatatat tacaagtata atcgaaagat
     1561 ttaataaata tttcaaaaat gtctcaaaaa caaagtccag atttacatca aacgcttcac
     1621 tcttaaagat attattcttg acaagcgaaa atgtgatgaa aaaatggact atgaaatata
     1681 aaaattgata taatatatac aatcatttat tcatatcttt tgatagtaga atataatttt
     1741 aaaaattata ccctaaaaat ttgctataat tgttataata tatataattt ccaaaaatat
     1801 ttaaaaacat cccaatatac attttttata atttattaaa ataaaattca tatattaaga
     1861 aatattaata gtaaataagc aattgttact ataacatagg aattatcttt aaaaacacaa
     1921 attaatttac attctctaat tttaaagtat atgtatatct ttgattttaa taaaattcac
     1981 ttaaaatatt ttaaggttga acaatgaaat tgaatgccca agaacttcct cctcttacta
     2041 atgttgcaat taattcaaac ccacaattgt attgaactga atctctacct actggtttcc
     2101 aactagaagg tgcattatat gagaaagaat ttgatgttgg ctctttcttt tgttgctgct
     2161 taattcctag ctcattagaa ataaatccag tttgatttat atgcaccctt ctatttgtta
     2221 cacgaatctg gccgtctgca atagaccagt ttccagaaac tttactaacg gaaatatatt
     2281 gaaatgctcc atctcctttt gatattgtat atgtaactgt accagatgct tttacaccac
     2341 cagttttatc ccatccatct tctgatatac ttgcttttct aagaggcttt attgtattag
     2401 tatcaaaaaa actgtcttag aatatatctt tgatgttaaa tttttagatt gtttaacttc
     2461 taataattga gttgttgata tagaatcaac gtatatggac tctcctgttc ttgcattttt
     2521 gattatacct ttttcattgg aacttttaac atcagtaatt ccatttttag ccatatcgta
     2581 taatttgtcc atgtctgtta cttgtttctc tgaatacatt actttcatag atgaatcttc
     2641 taccttacta gcacttgcat ttgttggtaa tagtattgtc attatgagta aagataaaat
     2701 agttgttaca gattttttta acctataatt catattaatc cttacttttt atttgttata
     2761 acagacattc tattgtaata gaatataaat ccacaatatt tttaaaatta gtattgaata
     2821 actattctca tgttataatt attcaaagga gggattgata tgaggaaaaa attttttata
     2881 ggtattctta tagttttaat aatctctgtg atagctatac ttaatataaa aaatgataaa
     2941 aatgtcttta ttggagaatc agaaaattgg aaagctaaat ttgtaactac tgaaggacac
     3001 tcaaaattag aaataaaata taaaaatagt aaagatgtag ttgaacaaaa atttaattat
     3061 tcctatcaat ttatagatgg tgaagatgca gttgtagttg taaaaaatga aattttagaa
     3121 gcaggtcaag aaaaagcaat aaaaatacaa agaccaggtc tttattcaaa tagagaatct
     3181 agttacaagc taattatatc ttggaatgga aatgaggaaa ctataaactt gaaaaagaaa
     3241 taaccatatt atcaattttt aaaaaattat tattcaaatt cttataaaaa gagtgtttag
     3301 aaagatgaaa cactcttttt tgtataagtt tttgaaaact ataaatgaaa tatatcaaaa
     3361 tcgttcgaaa aattactgaa ataaactctt aaatatatta taaattaagg tatattatac
     3421 atgaatttat tgggagttta tgagttttaa aattggaaac caaacaataa gggcgaaaaa
     3481 tacttcaagg aaaatatata aagttctata aattatcaaa aatatttcgc aaaaattgat
     3541 aattattaca aataaattga taatattaga aatatcgaaa ttgaacaaac taggattaaa
     3601 gctcaattag aaaaatccac taatcctgaa aatactttgt tattctcatt tattactgtt
     3661 gttttttcat tatttgctaa tttatttttc agttcattaa atgttaaata taactctata
     3721 aacttaattt tacagttaat atgtattata gttcttatat ttatatatat taatacattt
     3781 ttatatcgcc tcttcaattt gagccccatc attatataaa attaacctac ttgatatctt
     3841 aatttaatat atgtacacta ttcatatctt aatagtcaat attattcatt tttaatagct
     3901 ctttttagat gtcagaatta ttatattttg aaatttatta aatttttgca ttaaaaaaac
     3961 tagaaattaa tttaagtctt gaatta
//
LOCUS       DAELTA010000230         3941 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.235, whole genome shotgun sequence.
ACCESSION   DAELTA010000230 DAELTA010000000
VERSION     DAELTA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.235"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            178..876
                     /gene="tcdC"
                     /locus_tag="KPG13_003432"
     CDS             178..876
                     /gene="tcdC"
                     /locus_tag="KPG13_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG0987116.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            complement(1211..>3941)
                     /gene="tcdA"
                     /locus_tag="KPG13_003433"
                     /gene_synonym="toxA"
     CDS             complement(1211..>3941)
                     /gene="tcdA"
                     /locus_tag="KPG13_003433"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG0987117.1"
                     /translation="SENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFYFDP
                     IEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFE
                     YFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNPNNA
                     IAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYF
                     DSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAV
                     TGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYFNTN
                     TAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNE
                     FLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGIL
                     QNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFL
                     TLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAA
                     TGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPAN
                     THNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGW
                     QTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDA
                     NNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTI
                     DNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFG
                     NNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         1414 a          577 c          540 g         1410 t
ORIGIN      
        1 ctgacatttt tatcttattt tttcaaaata tattgaatct tcttacttta ttttgtaaaa
       61 ttatgcttag gggaaatata ttttaggaaa atatgaatat ataattttta gtcaactagt
      121 tattttaagt ttttaacttt taaaataaaa tatatctaat aaaagggaga ttgtattatg
      181 ttttctaaaa aaaatgaggg taacgaattt agtaatgaag gaaaaggaag ctctaagaaa
      241 ataattaaat tctttaagag cacaaagggt attgctctac tggcatttat tttaggcgtg
      301 ttttttggca atatatcctc accagcttgt tctgaagacc atgaggaggt catttctaac
      361 caaacatcag ttatagattc tcaaaaaaca gaaatagaaa ctttaaatag caaattgtct
      421 gatgctgaac catggttcaa aatgaaagac gacgaaaaga aagctattga agctgaaaat
      481 caacgtaaag ctgaagaagc taaaaaagct gaagaagcta aaaaggctga agaacaacgc
      541 aaaaaagaag aagaggagaa gaaaggatat gatactggta ttacttatga ccaattagct
      601 agaacacctg atgattataa gtacaaaaag gtaaaatttg aaggtaaggt tattcaagtt
      661 attgaagatg gtgatgaggt gcaaataaga ttagctgtgt ctggaaatta tgataaggtt
      721 gtactatgta gttataaaaa atcaataact ccttcaagag tattagagga tgattacata
      781 actataagag gtataagtgc tggaactata acttatgaat caactatggg tggaaatata
      841 actataccag ggatagctgt agagaaaatt aattaatatt gttttgtatt atagttaata
      901 ttttatatta tagtcaatat gtttaaagat gtttttataa ttgcaaataa acagttacaa
      961 ggctctaaat tagtttttgc ttttagcata ttatctattt tttatcaact attaattatt
     1021 tagtattaat atttccatat atgaatttta ttataaaata gtcaagaata atagattatt
     1081 aaatgataga aaaattttaa ctaaaagtca tgtattacaa taacacatga cttttaatta
     1141 aatctcaata tttattatat aaaaataatt tcttagtagc acaggaataa ttttttatca
     1201 aacatatatt ttagccatat atcccagggg cttttactcc atcaacacca aagaaatata
     1261 taacaccatc aatctcgaaa agtccaccag ctgcagccat agcagtatca ggcataaagt
     1321 aatatacttt accattaata gtttgccatc cagtaactgc ttttgaatta ttaccaaagt
     1381 aatatatttt tccaagtaaa tgtaggaatc tattttgata acgtatagct tgaccttcta
     1441 tattgttagc atccgtatta gcaggtgcaa agtattcaaa tccattagac cctttaaaca
     1501 ctcctatctg aggtaaacca tttctaaagt aaaaattttt attatcaata gttttataac
     1561 cattcgcacc catagctgta ttaggctcga agtaatatct attaccatca atagttaccc
     1621 aaccagtagc cgcttttgaa ttattaccaa aataatatat attgtcatgt aaatatagga
     1681 atctattttg ataacgtata gcttgacctt ctatattgtt agcatctgta ttagcaggtg
     1741 caaagtattc aaatccatca ggtcctttaa acactcctat ctgcataata ccatcagtat
     1801 taaaataaaa atgtttacca ctaataattg tataaccagt tgaagctata gaagtgttag
     1861 tattaaagta gtatttttta ccattaatag tttgccatcc agtaactgca acagcagtgt
     1921 tagtattaaa gtaatatttt ttaccatcaa tagttcgcaa tccggtaact gcttttgagt
     1981 cactaccaaa gtaatatttt ttaccattca aagttaagaa tttattttgg taaagtatag
     2041 cttgaccttc tatgttatta ttatgagtat tagcaggtgc aaagtattca aatccattag
     2101 gtcctttaaa cactcctatc tgcataatac catcagtatt aaaataaaaa tgtttaccat
     2161 taatacttgt ataaccagtt gaggctatga aagtgttagt attaaagtaa tattttttac
     2221 catcaatagt ttgccatcca gtagctgctt cagcagtgtt aagattaaag taatattttt
     2281 taccatcaat agtttgccat ccagtagctg cttcagcagt gttaagatta aagtaatatt
     2341 ttttaccatc aatggtttgc catccagtaa ctgcttttga gtcattatca aaataatatt
     2401 ttttgccatt caaagttaag aatttgttct ggtaaactat agcctgacct tctatgttat
     2461 tattgtgagt attagcaggt gcaaaatact caaatccatt aggtccttta aatactcctg
     2521 ttaccatttt agattcatta ttagcatcaa aatagaaatt atttctttca atagtaatat
     2581 atccattttg aagaattcca tcataactaa agtaatactt gtcattattt atagtgcata
     2641 gatgaattgc agcaatagca ttattaggat taaagtaata tttcttattg ttaataattc
     2701 tccatccagt aactgctttt gagtcactac caaagtaata ttttttacca ttcaaagtta
     2761 agaattcatt ttggtaaagt atagcttgac cttctatgtt gttagcatcc gtattagcag
     2821 gtgcaaaata ttcaaatcca ttaggtcctt taaacactcc tatctgcata ataccatcag
     2881 tattaaaata aaaatgttta ccattaataa ttgtataacc agttgaagct atagcagtgt
     2941 tagtattaaa gtaatatttt ttaccatcaa tagtttgcca tccagtagct gcttcagcag
     3001 tgttagtatt aaagtaatat tttttaccat caatagtttg ccatccagta gctgcttcag
     3061 cagtgttagt attaaagtaa tattttttac tatcaatagt ttgccatccg gtaactgctt
     3121 ttgagttatt atcaaagtaa tattttttac cattcaaagt taagaattta ctttgataaa
     3181 ctatagcctg accttctatg ttattattat aagtattagc aggtgcaaaa tattcaaatc
     3241 cattagaggt actaaacaca cctattttca ctacacaatc actatcaaaa taaaagtgtt
     3301 taccatcaat agttttataa ccattaaagg caatagcggt atcagtatca aagtagtatc
     3361 tactaccatt aacagtctgc caaccttttg agatgatagc agtgtcagga ttgaaataat
     3421 acttattatt gtcaattact tgcaatccga ctgcagcaat agcattatta ggattaaagt
     3481 aatatttctc attgttaata attctccatc cagtgactgc ttttgagtca ttatcaaaat
     3541 aatatttttt gccattcaaa gttaagaatt tactttgata aactatagcc tgaccttcta
     3601 tgttattatt ttgagtattg gcaggtgcaa aatattcaaa tccatcaggt cctttaaata
     3661 ctcccaactg catcacacca tcattattaa aataaaagtg tttaccatta ataattttat
     3721 aactagttaa agctgctcca gtatttatat caaaataata ttttttacca ttgatagttt
     3781 gccatccagt tactaagtta aattctatag gatcaaaata gaataatgaa ttatttatat
     3841 tgattaatcc tttaactaat ttactatctt catcatagta ataagtttta ttatctatta
     3901 ttttaaatcc taaatgatct ctatctaatt cattttcaga a
//
LOCUS       DAELTA010000231         3869 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.236, whole genome shotgun sequence.
ACCESSION   DAELTA010000231 DAELTA010000000
VERSION     DAELTA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.236"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..811
                     /locus_tag="KPG13_003434"
     CDS             131..811
                     /locus_tag="KPG13_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0987118.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
     gene            1259..1543
                     /locus_tag="KPG13_003435"
     CDS             1259..1543
                     /locus_tag="KPG13_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987119.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(2314..2529)
                     /locus_tag="KPG13_003436"
     CDS             complement(2314..2529)
                     /locus_tag="KPG13_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0987120.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(2540..3007)
                     /locus_tag="KPG13_003437"
     CDS             complement(2540..3007)
                     /locus_tag="KPG13_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0987121.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         1617 a          329 c          501 g         1422 t
ORIGIN      
        1 taaataataa agaaaatgga tttatagtat ttaaaatttc ttataagggt aaaaaatgct
       61 attagaaaat gtactaaaaa tatttttcca attctttata gaaaaataat gatatttagg
      121 agagattgta atgcaaattg atgtattgat aattgatgga gataagaata gttctcaaga
      181 gctgaaaaat tttttagaag gaaaggggat aaatgtctat ttagcatata attgtaaaga
      241 tgctattata aaaatattta gcaagaaatt tgacttaata tttttagaga taattttagt
      301 tgatggtgat ggttgggatt tttgtaaaaa aattagaaat ataactactt gcccaattat
      361 tcatataact tatataaatg aaaatcaaag tattttaaat gctttaaatt ctggaggaga
      421 tgattattta ataaagcctt taaatttaga aatattatat gcgaaagtta aagctatttt
      481 aagaagaaga gatttatttt taaataataa taaaaataat ataaaactag aagattataa
      541 tagagctagt ggagtaataa agttagaaaa taaaatgata aagttaactc caacagaaaa
      601 tagattatta aattacttta tagagaatgc tgaaaagact ctaactatta aggagatata
      661 tgaacatgta tggatgaatg agtatttaga tgataattat agtgtagtgg ttgctgtgag
      721 tggattaaga aagaaaatag agaaagatta taaaaatcct caaaaaatta taacgataag
      781 agaagtaggg tattacttta acaagatata aagctttata aacagaatga atgataaggc
      841 atagataata tgttagtata ttataagagt ttttgttcta acgatatatt tgtaaaatta
      901 gatttaattt acttaattaa attatagcag tagaataaat ataaaaaatg tctgaaatat
      961 tatagacatc agtaaaatat ataaaactat aatataagtc gtaataataa aacaaatgaa
     1021 tattaattaa taaaaagata gtagctattt ttatatagct attatttttt tattgttgtg
     1081 gatgaaaata tacttatatt ttactaatat acatttaaaa attataattt aaagcattaa
     1141 aaaataaatt tgtattttgt tttatataaa gtaatttgaa gaatatggaa aaacttgatt
     1201 agtacattat taattagggt ataaaatggt gtaaatagaa tgataagggg tggatgaaat
     1261 gcttgtatat aataaaagtt tttatcctaa tgacatattt ccaagattag atttttcaaa
     1321 aataaaaaaa cagttaaaat tgatagataa tgacctgtca gattttggaa gcatatgtat
     1381 aatagaaaaa gaacattata caataagtgt aaacagtata ggtgaaataa atgtgtatta
     1441 tgatttagag tacgaaaata aggtgtatag aatagtttat gagattgaaa agttatttaa
     1501 atctcaagtt ggaaggttta gcatatctac atacagaaat tgataattaa aaaagtagaa
     1561 attaaaaaac ttaatactaa agatatagat aaatatatga ggtaacagct agagagagtt
     1621 gttacccttt ttaaatacta agtatatatt attcttatta attttttgta aattcactaa
     1681 aagacctttt tatagaatat tattttcttt ttttattaaa taggtaaatc agtaaaaatg
     1741 caattccagt tccaattatt attccaatga tattttctat aattaatttt tttaatataa
     1801 aagtaaataa taaaaatgct atacataaac ctaaaatata tcctttaaaa taggttgaaa
     1861 gttttctttt catataatca ccaacttgta tcagatttag tttacttaat tagagtataa
     1921 cagtgttgta acttcaaaga gtatctaaaa gattacaaat gttagataaa atcatcaata
     1981 atgtaattta taacacgtag ctataaacta tgcaataata taaatggagg tataagtaaa
     2041 tgaaaaagtt ccatgttttg ctaacagttt aaataaatat taggtagaaa agaatgtata
     2101 taggaatctg tggtaaaatt atcaatttaa aagtattatt tagtgtgata cagttcttat
     2161 aggtaatgga taacaaatag ataaaggaac agtttctttc ttagaacata ttttgttatg
     2221 actttaaaca aagttaatat taatgtcaat aatatagtaa aagaataaaa atcatatagt
     2281 ataataaaaa agagtgtttc taaacactct tttttaatca ttttcatctt ctacatattc
     2341 aagtatatca cctggctgac agtctaaagc tttgcaaatt tcgtttagag tggtaaatct
     2401 aatagctttt gcttttccag tttttaaaat tgataaattt gcatttgtaa tatctatttt
     2461 ttctgccaaa tctttaagtg atattttcct ttttgccatc attacatcta ggttaactac
     2521 tattgccata acatcacctc tatatcgtta aatcattatc ttcttttatt tttatagctt
     2581 tatcaaatgt ttctgcaatt acaaagcaca ttagtgcaaa tataaaagag ataatcataa
     2641 gtggtgagat accgccacta tcagttccaa aaattcttat attattagta tttaaattat
     2701 aaccattgat acattcaatt atagtgttta tcaataaaca aaatcccatt accttaaacc
     2761 tttttacatt ttcaagtacg aatggggaat acttagtact attaattata aatatcaaga
     2821 ataagatgac tataaagtag tcagaaaaat atagtattct tgcaattaaa gcgattactt
     2881 tgtcagaact aaaatcatat aaaaacatag gtatcataca taagaatatt ataccagcaa
     2941 ctcctaaaag tgataataga aaatatagaa atactgataa tatattacta aaaaattttg
     3001 taatcataaa atatcatctc ctttatataa agtatattat tgattataat atacaattta
     3061 ttgaatttca ataataaatt attgatataa tatataaaac cattgtaata tacactaaaa
     3121 ataagaatgt ataaggtggt aaatttatat aaaaaagaat atgagttgta aaatattaaa
     3181 taatattgta ctaattggaa taattttaac tttattatag attattttaa taccattagt
     3241 actgatggtg tattttaaaa ttggtataag cattacaaat acgaatatgc caactgtaat
     3301 atgtaatata agtagctata tacatatgtg ctgtcccata tattgtatca ttgtttatat
     3361 taaaaagatt atgtaaattg gtatcaatca ataaatcatt ttcagaagaa atacctaaaa
     3421 acttaaaaaa tacctatatg tgtatttagt tagatttgag atttttacat ttaattttat
     3481 agtgacaata ttatattatt tttatgatgt ctatttatat gcaattacta taatatcaga
     3541 acttatagta tcatttgttt cattatcaat aaggtttttt actttagttt tgtcaatttt
     3601 attaaaaaaa gttattgata taaaagataa gaaggataaa actatatgac ggatttcaaa
     3661 cataagagtg gatatttata taatagatat tttatagtta attttattag attttattta
     3721 atactgtaat attaatatca caatatatta atagtgtatt aaagattgaa ttttgtagat
     3781 aactataatt tattgagagt gataaataaa aattatataa tattacagaa gaaagagagg
     3841 gagacaatat tatatccctc ttttttcta
//
LOCUS       DAELTA010000232         3827 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.237, whole genome shotgun sequence.
ACCESSION   DAELTA010000232 DAELTA010000000
VERSION     DAELTA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.237"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            115..444
                     /locus_tag="KPG13_003438"
     CDS             115..444
                     /locus_tag="KPG13_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0987122.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            448..1239
                     /locus_tag="KPG13_003439"
     CDS             448..1239
                     /locus_tag="KPG13_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987123.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            1369..2189
                     /locus_tag="KPG13_003440"
                     /pseudo
     CDS             1369..2189
                     /locus_tag="KPG13_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(2401..3594)
                     /locus_tag="KPG13_003441"
     CDS             complement(2401..3594)
                     /locus_tag="KPG13_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG0987124.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT         1436 a          492 c          591 g         1308 t
ORIGIN      
        1 atttttaagt ctattgtaga atataaacct tgtattatag agtactatgt aatattatag
       61 taaatataag tactatacat tatagagtag taggtgaaaa ggcggtggta cataatgcag
      121 ttaaataaag aagtgttaaa aggttatatc gatatattaa ttgtttctat attagaaaag
      181 aaagattgtt atggatatga aattgcaaag caagtaagag aaagaagtga gtttgaatta
      241 aaagaaggaa caatgtatct tgcacttaaa agaatggaat ctaaaaattt aattaaatct
      301 tattatagca atgaacaaag ttcaggtgga agaagaaaat attataattt aactaatgaa
      361 ggtaaagatt ttttagaaat aaagaaacaa gaatggagat ttattaaaaa ggttatgaat
      421 caatttttgg gagaggtgga ttaatttatg aaactaatag atgaatattt agataaacta
      481 tataaaaaat gtgacaataa aagtactata gaattaaaac aggaaatgag atgccatctt
      541 atagaatctg caaatgaatt taaattagaa ggattagatg aagaagaagc atgtaaaaaa
      601 gcaattgaaa gatttgatga tggtgatgaa atgcagtatg aattatgtaa tataataaaa
      661 gaactatctt tatcattgga taggcataaa tcaattgtaa tggggtttaa aaaagtatta
      721 ggttacataa gtataattgc atttttaatt tctggattta tgtggtatta caataatagt
      781 ttacaacata atatgtataa tttaggaaaa gaacttgatg gagaaataaa gcaattggca
      841 gaaagacatg atatgacaaa gattggtgaa tataagttag aacttgaaaa aatacttgat
      901 aaagataagt atagtaaagt caaagctctt agactatatg taatagatat gaaagatgga
      961 aacactaatt taagttcttc tggattgaat gctaatatgg tatatgagag ggaagcagat
     1021 tataataaca taagtaattt tatacagcat ttaggatata atggaaaaga ttttttagac
     1081 aagaatggga atatagtaaa tccagatatt ttcttagaat atttctttta ttttgaatcg
     1141 gagatgttaa ttccagtagc attcgtattt ggattattat gtataatagc gtattttata
     1201 ttaagattta aaatttcttt aataaaaaat aataattaaa taattcacca aataatttta
     1261 atttgtagag taaataagat gtttcaaaat aaaatttgaa tcatcttatt ttttattgtt
     1321 tgttatattc ttttctttta tatttctcca tagtggattg tatctataca taatcacatt
     1381 aaaaacaaca caacatatgt taactggaac gcagcagagg gagccaatac tgcttataca
     1441 ttggttacta aaaaaggtgc ttgttctgga atgtgtagga gcttgaaagc tttatgtgat
     1501 gctatgggaa ttgaatgtta ttatgtacat agtacaacaa atgaccatca gtggaatctt
     1561 attcgttttg aggatggacg tttgtatcat gtagatattg atgccaacaa atcagcagga
     1621 gcagacatca tatacaagag tcttactcac ccattaccat ttgacaagaa agcatatcca
     1681 gctgttggcg aagtagtaaa gacacagaat ccaacgccaa ctgttaagaa acctacgcca
     1741 gctaagcctt ctgcgccagt tggacctggg aatcacacta tgatagcaga tcaacaaggg
     1801 gcaatacttg atgaagttta tggaaataat ctacgtccaa aaattgaaat gtatgaggga
     1861 gttccgtgta gtttaatcaa ctcatcaata aatacaatta agtttacaag caaaaattca
     1921 aatttagacc catcaaagca tacagttact gtaatagggt ttatgaatac accatatggt
     1981 atggttgcat tagcaccaga gagcaaacct gttagttatc cttttgagta tgataaggca
     2041 ttttctttag attttactgg taagaacctt tctgcagcag aattatcaaa aaagtgtaaa
     2101 aaaataatat aattattaaa gtagaaattg gtgaaacaac atctaattct ggaatagggc
     2161 tagtagcata tactagatac gtgaattaat acaagtatac tagtatacaa atagaacaga
     2221 ctataaataa tgcaccctgt gcgaaagggt acatttatta ttatattagt cattttggaa
     2281 aataaaaaag gagttgaact taaaattaga ttagaaaaat taattttgag acaactccat
     2341 tttaatttat ttttaaatat aaaatcattt attttaaata atattatgtt tgattaatgg
     2401 ctatttaact aattttgcaa attctgcacc aaatgccata cactctttta atgtctcttc
     2461 actagggaag aattttttct taagagattc tacaggcatt ttgaatgaca tagatttaag
     2521 taaagtctca gccatagtta taccttcacc actccaacca aatgaaccaa atactcctgc
     2581 tatcttgcct tggttagcca ttgggtctat aacagagaat aaatcccaca taggtttaac
     2641 catagttttg tttatagttg gagagcctag taatattact tttgacacta ctaatgtatc
     2701 gtgcatttct gttaatgtca tatcttctaa atcatataat tcagcctttg caccttcttt
     2761 atcaagtcct tcttttatct ttttagccat ctctaaagta ttactataag ctgataagta
     2821 gaatatagaa acttgatttt gattagttgt attaacagct tctgtagacc actcaacata
     2881 tcttttaaca gcagccattg ggtctttagt aagcattgga ccatgagaag tcaatatagt
     2941 atcaaattct atgtttagtc ctaccacctt atctacagca cttaaaacat gtttagcaaa
     3001 tggcttaact atacaatcat agtagtgttt agctgatttt agataatctt cactattcac
     3061 tacctctgca tcaacacttg caaagtgaca accaaatgca tcacaagtta aaagagtctt
     3121 gtcctcctca atgtaagtaa acattgtatc aacccaatgt aagaatggag cagttataaa
     3181 tctaagattt cttttaccga tatttaaaat ttcaccatct tttattacat gacagttaaa
     3241 aggtctattt atttgaccat ctaagtatag tttagcagct ttagtacaat aaacttctat
     3301 attaggattt atatctaata agtattttaa actacctgca tggtctggtt ctgtgtgatg
     3361 aattacaaca tagtcaattt tagctatatc agtaacctct gataaattac ttaaaaactc
     3421 atctttaaaa tttgccttta cagtgtcaaa taaaaccgtt ttttcgtcct tgattaaata
     3481 agagttgtaa gttgtcccaa actcagtttc cattattatg tcgaatactt ttaaaccttc
     3541 atctactact ccagtaaagt agatatcttt ttttacttca aaaactttac tcatttttaa
     3601 ccctcctaaa atttatcatt atataagtaa cataccccat tttgaggtga atgaaacaaa
     3661 agatgtcatg ataagtcatg taaaacaata gttttatgtt tataatatat catactttgt
     3721 aaaaaaattg tatttcaata aaaatttaat agaaaatatt cttaaatatt tttagctatt
     3781 ctattttgtg aataatatgt attttaaatt caatatttta gttatat
//
LOCUS       DAELTA010000233         3798 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.238, whole genome shotgun sequence.
ACCESSION   DAELTA010000233 DAELTA010000000
VERSION     DAELTA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.238"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            326..2236
                     /locus_tag="KPG13_003442"
     CDS             326..2236
                     /locus_tag="KPG13_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG0987125.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(2299..2922)
                     /gene="ytaF"
                     /locus_tag="KPG13_003443"
     CDS             complement(2299..2922)
                     /gene="ytaF"
                     /locus_tag="KPG13_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG0987126.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            3110..3754
                     /locus_tag="KPG13_003444"
     CDS             3110..3754
                     /locus_tag="KPG13_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0987127.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         1650 a          376 c          565 g         1207 t
ORIGIN      
        1 aaatgaaagt ataagcttat ttttactata aaaattttca ctgtacaaaa acattaatga
       61 cattattttt attgacataa atatataatt tatggaataa tattagaagc actgatataa
      121 gtgtttaaat aagaatagaa aaggagaagt gttttatgaa aaaaaatact aacattaatg
      181 agtccggagt taaacttctt aataaataag gagtttgata tataataact ccttatttat
      241 taagttgttc gcaaaataac tattatcaaa gtaaatgata gggtgattta aaagacgatg
      301 acttataaat aaaggagaaa aaataatgaa tagtaataca tttgaaagat tacaactaaa
      361 tgaagttaaa gacttaataa aaatacattg tgtaagttcg ctaggaaaaa atttaataga
      421 taaattaact cctagtggga atattggagt agtaagaagg aaattagcag aaaataaaga
      481 agctagaaag ataattgaaa atagtaatca cataccactt gaaggattgt ttaatgtagg
      541 ttctacaata gataaaattg aaaaaggaat gataatagaa cctgttgaac ttgtaaatat
      601 tgaagatttt ttaagaggtt gtaggaagat gaaagctttt atgttagaaa aagaatttta
      661 ttcacctaca ttaagttctt atgccttaaa tataacagaa tgtaaaagta tagaagatga
      721 gataaattac tgtataaaat ctaataaagt tgattctaat gctagtaaag aattaaaaaa
      781 gataagacga aatatagaaa ttacagaagg aaagataaaa gatagattaa ataaatttat
      841 aacatcaact gtaaataaaa agtatataca agagtttata ataagtaaaa gaaatgatag
      901 atatgtaata ccaattaaat cttcttataa aaatgaagtt aatggtacta tattagatac
      961 atcatcaaaa ggaaatactg tatttattga acctattagt gtttcaaatt taagtacaga
     1021 gttaactatg ctaaaagctg atgaaactat tgaagaatac aaaatattat cttatttaac
     1081 agaacttata tttgagaaga taagtcagat aaaattaaat atagagatac tttcagaata
     1141 cgatatggta tttgcaaaag ctaagtatag tcaaaaaata aaaggaatta ctccaaaaat
     1201 aaataataat ggctatataa aaataattaa aggtaaacat ccacttctaa caggagatgc
     1261 agtaccttta gattttgaaa taggcaaaaa ttatagaagt cttataataa caggtcctaa
     1321 tgcaggagga aaaactgtaa cccttaagac tgttggttta ctaactttaa tggttcaatg
     1381 tggatttgat atttctgcaa aagaaggtag tgaatttagt gtatttgaaa aagtttttgt
     1441 agatataggg gataatcaaa gtattgaaaa tgccttaagt actttttcat cacatataaa
     1501 aaatattgca gagattatga gtttatcaaa taattctact cttgttctgt ttgatgaaat
     1561 aggaagtgga actgaaccta atgaaggagc tggtttagca atatcattat tagaagagtt
     1621 ttacaaaatg ggatgtataa ctatagcatc aactcattat ggagagataa aaaagtttgc
     1681 tacattgcat ccagaatttg aaaatgctgg tatgatgttt gataaagaaa cgttagaacc
     1741 attgtataag ttaactattg gaaaatctga agatagtaat gctcttttta tttctaaaaa
     1801 aatgggaata aaaaatagag ttttagatag agctaaagaa tatgtaattg atagaaacta
     1861 caatttagat ttagttaaaa gaagtaagct acaatctaat attgaagata aaaatagttc
     1921 tgaagtcatt ttaaactata cagattataa tgtaggggat aaggtaaaat tactagatga
     1981 agatgatttt ggaattgtat ataaacctat ggacaagttt aataatgtag aagtattgtt
     2041 taatgataat tttatttcag taaatataaa aagattacaa cttagcataa aagcatcaga
     2101 attgtatcca gaagggtatg atttagatgt attatttact agttttaaag aaagaaaatt
     2161 agaaaaagat attcaaagag gttcaaaaaa agctcttaaa aagattcaga aagaaattag
     2221 agagaataga aaataaatat aaaaagcaca aaagaaagca atctaaagag aaaagtttat
     2281 ctttagattg ctttcttatt aacatatctt cataaatcca atgactaaca agattaaacc
     2341 acttaaccaa gataagttta aatttacgtt tttaacaaac atatttccta gtaaacaacc
     2401 aagtaatact gcaattatat ttgatattaa agaaaataga ataacttgta tataattcac
     2461 acctacaagg ccactaccaa aaccgatagc taatccatct aaacttaaag caagtgcaag
     2521 tgtaaatgct tcagcagatt taagcacttt agatttactt gtatctaaga catcaggttc
     2581 gatacttaca ttaaaattta atttaaggtc taacatatta aaattataat tctttgagtg
     2641 tatagatttt ttcttaaaat aagatttaaa aaaactttca attagtctag cacttcctaa
     2701 taaaaataaa ataaagaaac atattaacat tgttatattt tgaggtaaaa agtctctaac
     2761 tattgtacct gcaaaaagcg atattgctaa aatagctgaa caaactacgc tgataattat
     2821 atttgaatat aagggaactt taactttatt tacgccatat ccaaagcttg tcacaaaggc
     2881 atctatacaa agagaagtaa ctaataaaat tgattcaatc acagtagatt aacatccttt
     2941 ctcttaaatt ttgtacttat attagttata gtattgtaaa aatggataaa ttgttacaga
     3001 taatccaata aattgttgat aaaattccaa catcatacaa cacctaaata acaaaagaat
     3061 catatcatat aataatataa aaccaatcat aataaagcgg gtgaaaataa tggaacaaaa
     3121 taataaggtt gggaatttcg gtgatttgtt gaagatttta ttagatgaaa aagaattatc
     3181 aatgggagaa ctaagtaaaa aaagtggaat tgataaatct actatatctc gtatagctaa
     3241 taataaacaa aagccaaata taaatcattt agaaaagatg gcaatacatt taaatattaa
     3301 cctagaagaa ctgttgaaag cttcaggata tgaatttaga aacagcaata atcaaatatt
     3361 taatgcagat agtgatttta gtaactttga tgatattctt ggatttgcaa atttaatcaa
     3421 tgataagaac tttaatggaa atattgaaaa agagttaagt aagtgtaagt tatatgtcca
     3481 aacagatgaa ggtaaaaaat tactttttga taattttaat aaaaaaatag attctattga
     3541 aaaacaaggt caatttacag atagattaag acaaatgtat gctgattttt gtacagatgg
     3601 acttacaata aagaaatatt tattgattgg aagtatgcta ctttattttg ttatatcaac
     3661 agatgtgatt ccagacttcg tattcccaat aggatttatg gatgatttag tagcattaaa
     3721 tatagttaca aaattactta aaaatgataa gtaagtttta gaatcatatt taatatataa
     3781 ataaatcaaa gtatataa
//
LOCUS       DAELTA010000234         3797 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.239, whole genome shotgun sequence.
ACCESSION   DAELTA010000234 DAELTA010000000
VERSION     DAELTA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.239"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            519..2330
                     /locus_tag="KPG13_003445"
     CDS             519..2330
                     /locus_tag="KPG13_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG0987128.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2418..2999)
                     /locus_tag="KPG13_003446"
     CDS             complement(2418..2999)
                     /locus_tag="KPG13_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987129.1"
                     /translation="MSYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNY
                     TNYYMEFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(3435..3641)
                     /locus_tag="KPG13_003447"
     CDS             complement(3435..3641)
                     /locus_tag="KPG13_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987130.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT         1450 a          378 c          510 g         1459 t
ORIGIN      
        1 tccatttata aattttattt ttaaatataa ttaataagag ataagtaaat tattggtctt
       61 attgcacaaa aatagatata atttaagtaa tataccaaaa aaaaggaata tatttacata
      121 atatgataaa ataaattcaa agagttaaat tgtaggtaaa tagtatttta attatttatt
      181 aaaatataga ttatgtatat gccaaattta aaaagagaca taaagacatt atacaaatat
      241 attattgggg tgatattttg taagaaaaga agtgatgcag taaattaatt gagtaattta
      301 aaaatgattt aatgataatg ttgatgaatg tttaaaaaga aattttagaa aaacgtatat
      361 tgctgtttgt gttggaggtg atgaatttgt agaatttata gggtataaat gattaaacta
      421 tttaagataa atacatatca ctgtaaaata ttgataatca ttttttataa gagattgatt
      481 atttgcaaat tatatatatt acaaatgagg taacaataat gaagaaaaaa tatgattttt
      541 taatttattc tgctatttgg ctagttatta ttattctttt tatattttct ctatatcata
      601 gtatagagca tattaaaatt ataagtcaca ctggtactat tcgcagtgaa actcaaaagg
      661 ttgttaagca agaattgaat aatgaaagaa atgatgactt gataaaacga ctggataata
      721 ttctgataaa gctaagaaca ggtaatggag aaaatggatt tcaaagatgt gataataaag
      781 aatttcaaca aaaactaaat caaatggatt ctatgtggga atctatgaag aaagaaatta
      841 taaaagttag aaatggtgct tcaggtgata aattatataa attgagtgaa gagtattctg
      901 tgttgtctaa tcaaattgtt tttatatctg aaaaacactc taatgcaaaa ttgtattctt
      961 ttgctactgc actatttatt tatcttatat tctcaacaat aagtctttta atttgggaat
     1021 attataataa aaaaaggttt aaaagaatat tttatactga taatttaaca aaaataaaaa
     1081 atcaggttgc atttgaaaac agagcgattg aaattttgca caatgcatct aacaaagagt
     1141 atgtactgct aaatattgat attgacaatt ttaaatatat taatgataca catggatatg
     1201 aatatggaga taaagttcta ataatagttg caactgttct ttctaaaact tttaatataa
     1261 aagaaacttg tgcaagaatt tgttcagata attttgtaat acttgcaaaa tatagagatt
     1321 ctcttttaga ggatattcgg gaaatgctaa ctaatgctat aatctctgaa ttagatatga
     1381 atgtaactca aactatatct tattgtatag gagcttactt agttgaaatt gataatttag
     1441 gttataaatc tatcaattct atgatggata aggcaaatat tgcacacaag gtaagtaaaa
     1501 caagaggaat ttcctccaca gtatggtata atgaaaatct gttaaaacag ttacaaatgg
     1561 aaaatagtat ttataattat atgtataaag cgatagaaaa tgaagaattt catatgtatt
     1621 tgcaacctaa atttcaaata tcttctttaa atgttgtgag cgcagaagct cttgtaagat
     1681 ggttttctcc agaacttggt tttttatcac cagatgaatt tattccattg tttgaaaaaa
     1741 gtggatttat tattgaattg gacttttata tgttaaaaaa agcgtgttca tttgtaaaaa
     1801 aaacgtttat gaaaaaaaat caatatacat atccgattgc agttaatttt tcaagggtta
     1861 ctatttacca aaatagtttt tatcaaaggt ttttagatac tgttaaagaa tatgaaattc
     1921 cttttaagta tatagagata gaggtaactg aaagtgcttt taatgaaata tctcaaccag
     1981 ttatttcaat tttagaggaa cttaaaaagt taggattttt aatttctatg gatgattttg
     2041 gatcaggata ctcttcatta agtcttttat gttctctatc tattaatgga ttgaagttag
     2101 ataaaagctt acttaaagaa acatttaacc gggagaaagt atatagtatt attcagtgta
     2161 ttattgaaat gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa
     2221 aagacttgga atttttaaat acagttaaat gtgatgtagg acaagggttt tatttttcta
     2281 aaccgataga agaaaaagaa ttttttaaca aatatgtaac taaaaaataa tataattatt
     2341 tttatcagtc tatacattga gtcattttat atctcgttaa atgactcaat gcactgttat
     2401 gtattttaaa atataaatta tttaaaacgt tttttatact tatatagaaa ttcttctata
     2461 ttttttgatt gttctcttat ttcaactgct tctatatcta gtaaataaag tatagaatca
     2521 tttgtaagtt caacatattc accatcttct gaaccgagtg catgtctctc aatttttaat
     2581 cctttttctt cataatattt ttttgtttct atatataatg attctcgctt ttgtatgtag
     2641 tctttcactt cattatttgg aaagttatat gaatccacta atgtttcgcc atttataaaa
     2701 gttttctcgg tagcaacttt ttcatcccaa caatggattt cttcccaagt aaaaaatatc
     2761 tcagttccat ctggaatgaa tgaaaacctg gtaataatat ataaataatt attgaaattt
     2821 acttcataat attcaggaat agcttcaaat tccatatagt aatttgtata attaaagtta
     2881 ctactatctt tttttacaaa tggtattttc tcaataaatt tatatattga ttgttcattt
     2941 ttaaaaattc ctatatcata gcttctttca aattgattaa tttttaataa atatgacata
     3001 tattctcctt atttgttgaa atataatttt tataactcta ctaattttaa acatttttaa
     3061 ataaatattt gtatatacct tatataatat tatagtaatt agttttaaat caatagaata
     3121 gtatgtattt atctaatgcg gcaatataat ttaaagctgg tgttgtagaa aaactaagag
     3181 tgaatatagc aaagagaagc aatagtattt taaactttgt ctctcttatt ttttgaaaac
     3241 taacattaaa acataaggta taaataatat tatcatataa atgaaaacaa atttgaattt
     3301 ttctctggtc gatgtatata gtaagtttgt tgatatgaga aaaattatta ttgataatat
     3361 acatgcatag aaaggaaatg gtatataata ttttcttaag tttctttttt aggcatttct
     3421 attggcattt taggctatag tactaaatac attaaccaac aaaataatga aataaatata
     3481 tatatagaaa acttaatttt cgtgggaaga aaaaaagcaa taatagcaag tattattaga
     3541 gaataacgaa taatacgagt aacatttctt tctttttcta tgatagttaa atcccagtga
     3601 ttttttataa attcaaggtc tttttcttga ctctgattca tttaatcatc tcctgttaat
     3661 agtataattt taaagctatt actttgtttt attttaacat atttatacac taaagtgaat
     3721 tttatgatta gatttattta attatttctg aattaaaact tattagaatt ctctattttg
     3781 ataatttcaa actctat
//
LOCUS       DAELTA010000235         3788 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.240, whole genome shotgun sequence.
ACCESSION   DAELTA010000235 DAELTA010000000
VERSION     DAELTA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.240"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(39..281)
                     /locus_tag="KPG13_003448"
     CDS             complement(39..281)
                     /locus_tag="KPG13_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987131.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            493..2316
                     /gene="typA"
                     /locus_tag="KPG13_003449"
     CDS             493..2316
                     /gene="typA"
                     /locus_tag="KPG13_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG0987132.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(2406..3314)
                     /gene="trxB"
                     /locus_tag="KPG13_003450"
     CDS             complement(2406..3314)
                     /gene="trxB"
                     /locus_tag="KPG13_003450"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG0987133.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
BASE COUNT         1310 a          602 c          519 g         1357 t
ORIGIN      
        1 ttgcgagtat tacatattaa ttttatatat tattaatact acatgtacgg tggtatctgt
       61 tgttttctca ctatttccaa ttccttaatt tctaaagact taatcacatt tactttatag
      121 ttttctttag actttatata caaaacatct tctatctttt taccttcaat aaatttatga
      181 gaccacttaa gaagttcttc aaaattacaa acttcttctt ttacaaaagt atcactttca
      241 cttgcaaaat atttaattat tattttggtt acttccaaca tatatcatcc tttactcttt
      301 tattaatctt tttatctatt aaggttatta tttgtctaaa aaattttttt aaacaaaata
      361 attttcagat gcaaaactaa cagttttatt caatttaaac tatattaata caatttaata
      421 tattcctcct tgtacctaat aatatattgt gctataatta ataagttatt ttaatcgaag
      481 ggagcaacaa aaatgtcaca aaaacataaa attataaata tcgcagttat agcccacgtt
      541 gacgctggaa aatcaacatt ggttgatgct ttcctttctc aaagcggtgt ttttagaaag
      601 aacgaagtag ttaaagactg cgttatggac agtaacgacc ttgaaaaaga aagaggcata
      661 actatatatt caaaaaactg tgcaataaac tatgaagatt acaaaataaa catagttgat
      721 actccaggac acagtgactt ttcttctgaa gttgaacgtg ttatgaaaac tgttgacaca
      781 gttattctat tagttgatgc tagtgaaggc ccaatgcctc aaactagatt cgttttacaa
      841 aaatctttag agttcggttt aaaaccaata ttatttataa acaagattga taaaaaagac
      901 cagagagcgg aagaagtagt taatgaagtt ttcgatttat ttgtagactt aaatgcaaca
      961 gatgagcaat gcgaatttcc tataatatat ggtattgcta aacaaggtat tgctaaactt
     1021 gaaatggatg atgatagtga agacctttct cctctattta aaactattgt aaatcacgtt
     1081 gaagcttatc ctaattacga taatgaacct ctacagttcc aaatatcagc tcttgcatat
     1141 gatgattatg taggtagact tggtataggt agaatttaca aaggtactct taaaaacaat
     1201 acacaagttg ctatatgtag agaagattct gttgtatcta aaggaaaagt atcaaaactt
     1261 tctgtgtatg aaggtttaaa acaagttgaa gttgatgaag ctactagtgg agaaatagtt
     1321 gttatagctg gtattcctga tatatctata ggtgaaacta tatgcgattt agatagtcca
     1381 ctacctatgg aaatgataaa aatagaagag cctactcttt ctatgaactt cttagtaaac
     1441 gactctccat tcgttggaaa aagtggaaaa ttcgttacaa ctagacattt gaaagataga
     1501 cttgaaaagg aacttgaagt aaatgttggt cttaaagttg aacctcttga tactactgat
     1561 ggatacaaag tttctggacg tggagagctt cacttatcta tactacttga aaatatgaga
     1621 cgtgaaggtt atgaagttgg tgtttctaaa cctgaagttt taatgcataa agaagatgga
     1681 aaattaatgg aaccaataga aagagttgtt gtaaactgtc ctgaagttta ctctggtact
     1741 attataaatg aattaaacat gagaaaaggt atgatggagt ctatgtcaat agaaggagac
     1801 tatgtaaaaa tagagttctt agcacctact cgtggtcttt taggatatcg aagtgaattt
     1861 ataaacgcta ctcgtggaga aggtacttta gttcgttcat ttgaaaaatt tgaagaattt
     1921 aaaggagaaa taccttcaag aggaaatggt gttttaatag cacaaggacc tggtgttact
     1981 atggggtatt ctcttaatgc cttaagtgat agagctgtaa tgtttgtaga ccctggtgta
     2041 gaagtttacg aaggtatgat aattggtatg aattccagaa aagatgatat ggtagtcaac
     2101 ccttgtaaaa acaagaaaat gagtaacgta cgtgcttctg gttcagatga tgctataaaa
     2161 ttatctcctc ctagaatatt tactcttgaa gaagcactag agtttataga agatgatgaa
     2221 ttagttgaaa ttacacctga ttctataaga cttagaaaaa gattcttaaa tgaacatgat
     2281 agattaagat ataacaaatc tagacaaggt aaataaaacg tacatttacc tctaattaac
     2341 aaaatagcca tatagtactg aaaaattatt tcagtttata tggctatttt tatttgaaat
     2401 ttatattatt caacatttat ataattagct gcttgaacgg ctgcaatagc tccatctgat
     2461 gctgctgtta ttatttgacg taaaaactta gttctactat ctcctgctac atatacacct
     2521 tctactgatg ttatacaaga ctcatctgaa attatatatc caccttcatc caaatcaaga
     2581 acattttcaa acattttatt attaggttta agacctatag caataaaaat accactaaca
     2641 tctatagttc ttttttcttc agtctttaag ttttttactt ctattttcga tacagtcttt
     2701 tctccttcta ttttttctac accatgactg taaagtatct caatattctc tcttgctttc
     2761 acagatttct ccaacacttc ttcacctcta aaactatctc ttctgtgaat taaatacact
     2821 tttgtacaat tattagctaa aaataaagca tcttctagtg cagtatttcc tccacctact
     2881 atagcaactt ctttatcttt aaaaaaggct ccatcacatg ttgcacagta agatactcct
     2941 ctacctgtaa attcttgttc ccctgcacaa cctaatttac gtctttcaac tccatttgca
     3001 agaataacag cctttgcttt atgttcacct gaagaagttt ttacaacctt tacttttccc
     3061 tctaaattta tctcttcaac ctcatcaaac tgaatatcaa caccttgagc aactgcttga
     3121 ttgtatatat tattagaaaa ttctacccca gaaatctttt gaactgctgg ataattttct
     3181 acctcagagg tacttgcaac ttgacctcca taaatatttt tttcaaatac tgtaacgctt
     3241 aaccctgctc tcatagcata aattgctgaa gttaaacctg ctggtccagc accaattaca
     3301 atgatatcta ccataacgtt tacctctctt tatattatta tttcttatat attattctca
     3361 tcattcttat actttactat tattatccat atatactata aacaaaacat tattttttat
     3421 taattaactt tattttacat atatataaaa aaactgcaaa taaaaattaa cttttattta
     3481 cagttctcac ttatttatat aattcaaaac cttattggtt atcatttaca gtgtcttcta
     3541 atatattgct ttctgatata tatgatagaa ttgaaagtcc atacataacc aaaactaatg
     3601 ctgctgttat aagtactatt cccatattta atacctcctt ttgtttacag tatactatta
     3661 ttatgtgaaa tatttatgaa tttaatttga atatatatgt aatttcacac taacaatttc
     3721 ttcaaattaa acattattaa ctatatactt ttgttgaacc aattatgatt aatagtttaa
     3781 atattagc
//
LOCUS       DAELTA010000236         3787 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.241, whole genome shotgun sequence.
ACCESSION   DAELTA010000236 DAELTA010000000
VERSION     DAELTA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.241"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..925
                     /locus_tag="KPG13_003451"
     CDS             <1..925
                     /locus_tag="KPG13_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0987134.1"
                     /translation="IAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYS
                     SNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERA
                     IMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEK
                     VTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANA
                     IDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIA
                     KKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            964..1449
                     /locus_tag="KPG13_003452"
     CDS             964..1449
                     /locus_tag="KPG13_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987135.1"
                     /translation="MYLKRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(2104..2328)
                     /locus_tag="KPG13_003453"
     CDS             complement(2104..2328)
                     /locus_tag="KPG13_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG0987136.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2425..3678)
                     /locus_tag="KPG13_003454"
     CDS             complement(2425..3678)
                     /locus_tag="KPG13_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG0987137.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         1505 a          485 c          491 g         1306 t
ORIGIN      
        1 aatagctgga tttatatatt ggtatagttc taaaaagttt aatattaagg tcatgaactt
       61 tgataaatat atagcaattt tgatgattcc attgttgctt ataatattat ttatgcaatc
      121 atttgaatat agttcaaata taaatataga tacaagtctt gggattgtaa agctgctatt
      181 aaatacttct ataactgaag aaattcaagc ttttcttttt tctataattg gaactatatg
      241 tgtatttttt tctctattta cctttaagaa actaattcaa gctctcgaag atgacaagga
      301 gagggcaatt atgaatcaac agattcatgc tcaaaaaaac tatattgaag aagcgaaatc
      361 acgattatca caaactatat cctttagaca tgattttaat aatcatttag ctattgtcaa
      421 tggattgttg aaaaaagacc aaattttaaa agcacaagat tatttaaata agcttgaaaa
      481 agtaacaagt gacctatcat tttcttgtaa tacacagaat attgttattg atgctctttt
      541 caacaataaa ttaagtatag caaaacaatt aggcatagag gtagattgtg aagtaattat
      601 accccaaagc actaacatga acgattttga cctatgtata gtgtttgcta atgctattga
      661 caatgcaatt aaagcttgta gagttgtaga taaacaaaat aggtatttaa agctttccac
      721 taagttagag ggtggatttt ttatgataga aataaaaaat agctataatc caataaataa
      781 ttatacaagt ggctcaggta taggtctttt aaatataaaa gaaattgcta aaaaatatca
      841 aggggctata agtatagata agacatcaga ctattttcaa ataaatgtat tgctagttat
      901 ttcattacat tgcgctagta tttgagaaca atttcattga aaatagtatt tgtaagctat
      961 attatgtatt tgaaaagaga aagtttgata tatagaaatg aggaggttac taaaatgaaa
     1021 gttggaaata taaatggtca agcaccaatt acaaatggtt tagatagttg tcaaagtgag
     1081 gtacaaggct tagaacaaaa gaaaaaagca ttacaggatg aaatagagaa agtaagaagt
     1141 gataaaagta ttgatggtga aacggccaaa ttaaaaatag aagctttaaa aaaagaaatg
     1201 gaagagattg aaaataaaat tcaagagtta aaaggaaata aaggaaaaaa aacaaataaa
     1261 gaagaaaata acgtttcaga agtaaataat aatctattag agagaaaaaa agaaccttct
     1321 aataatgaaa atgtaaaaca attatatgat gtttttgaaa gaagcaatgt agacgaacaa
     1381 tgggataata cttatcgtgt tctccaagat gataaaaact taaaagtaaa atttaataga
     1441 cctatatagt ttatttataa aaatagttaa tattttaatt atcaccactt tttagaagaa
     1501 tacacacttt attttaaaga cccacatatt tatttacagt tagcacattt atatgtggtt
     1561 ttataactat tatttacaag tctatattga taacttttac atagtatatg atgtttaaat
     1621 acttattcaa atttatctat acataaacaa aaaaagagat tttaaaatct ctttttagat
     1681 tgttgactta tggctttttg atttaataaa atcaagaagc cgtttgaatt ttatatgttg
     1741 gctgataccc ttctgatatt gaaacaccat ttttgtttcc aaaatcgttt aaaattaaga
     1801 acttattatg aatgactatt tcattatgaa caataagcaa ataacatgaa tcttcaatca
     1861 atgttttaac agtagcatta aatgcttgct tatatagttc tatcgtttgt ttgaactgcc
     1921 cattaaagta aatatgagtc aataacataa ctatatctac acaaattaat ataaattttt
     1981 tatactgtac attatctaac tgttgattat ctgtattaca acatgattga atttaaaaag
     2041 ccatcaacac atagaatgtt gatggcctga aaatagattt taaaatctct tttttattat
     2101 atattaatta ttttttaaat ttatattttt tgcatcagat tttctaatag ctaagtcaaa
     2161 ccctctaaat ctaatcacta tagggtcatt aagaggagct atctttttca cttcaacttc
     2221 agtgccatct attagtccta aggctagaag tctcttcata agtttttcat ttccagctat
     2281 attgtctata attccctttt gtccaagttt taagttgtaa acagtcatta ttaacacctc
     2341 ctaatgaaaa tcattatcaa actttatatt tataatatat aacaaaaaga gcaaattgtc
     2401 aatatataac aaaaaataaa catactaagt tagtatgttt ataaaaaata gtatacatgg
     2461 gatactaata gcatctagca aaaaaccact tactaatggt acaactaaaa aggcttttgg
     2521 agaatcacca tatttttgtg tcaaagaact catatttgcc agagcgttcg gagtagctcc
     2581 tattccatgt ccaatcaaac cacttattat gatggcagaa tcaaaatctt ttccaagtac
     2641 tctaaagcaa ataaatattg aaaataggat tataaagatt acttgaaaag ttactattaa
     2701 gagaatagga ggtaataatg tagataattc aaataaatct atattcataa gtgataaagt
     2761 taaaaaaatt cccaatgaaa gttcttttaa aaaatctata agtttaaaat ttaattctag
     2821 tatatttatc ttatcattta aatttctaaa aataacagcc gaaaacatac aagttacaac
     2881 agagggtaat agtatattaa acgttatata gaagcatcta cttataattt ctcctgtatt
     2941 tatacaaata aatatgagaa gaacctgttc caaaaataca gatggtgtta ttgtttcgga
     3001 gaagttatta ttttttatcc tattcttatt tctaaaaaat ttacttccaa atctctttag
     3061 atttctatta tatctaggaa tcgacaagtc tatatttgat gatggtttta atttatattt
     3121 atttattaga aatttagcta caggacatcc caatatacca cccataataa gtcctattgt
     3181 tgcagcagat aatccaaccc caactgctcc ttctatacca agattttcta ttgtaagtcc
     3241 aaatgcagca gaataaccat gaccaccttc catagataca tttccacaca taagccctaa
     3301 aagaggttca agttttataa tctttgatat tgtaatagct aatatatttt gacaaaatgc
     3361 caatactcca cacaaaatcc aatatctaaa taatacacta ccacctttct ttataagatt
     3421 tatgcttatt tcaagtccta tagttgtaaa aaagaaggtt atgaaatttg gcattaaatt
     3481 tccacttata gatatatctg ctatattaaa atatttaaaa aataaattta gaaaacaaaa
     3541 taatagacct cctattactg gtgcaggtat acagaaatta tttaaaaagt taactttatt
     3601 cttcaataaa ttaccaatta aaaagaagaa tatagatagt gtcaaggttt gcactatatc
     3661 aaaacttaag gtaatcaaaa taagtcctcc tttgtaattc aaagttttaa ttatataact
     3721 taaattctta tgtattgaaa ttttattatc aaatgtatat taataaatgt atattaaaaa
     3781 gaatcag
//
LOCUS       DAELTA010000237         3753 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.242, whole genome shotgun sequence.
ACCESSION   DAELTA010000237 DAELTA010000000
VERSION     DAELTA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.242"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            68..1120
                     /gene="ytvI"
                     /locus_tag="KPG13_003455"
     CDS             68..1120
                     /gene="ytvI"
                     /locus_tag="KPG13_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG0987138.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1210..2436
                     /gene="pepT"
                     /locus_tag="KPG13_003456"
     CDS             1210..2436
                     /gene="pepT"
                     /locus_tag="KPG13_003456"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG0987139.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2643..3695
                     /locus_tag="KPG13_003457"
     CDS             2643..3695
                     /locus_tag="KPG13_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG0987140.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1453 a          395 c          699 g         1206 t
ORIGIN      
        1 attcaaataa aactagtatg aaatggacaa aataaaaata taaattagaa gaaaggagat
       61 ttttaagatg agcatactag aaaataattt tttatacaaa ttaaaaaaca acactatctt
      121 tttttgtata tatacattgt tatttatttt tatttacaaa gcgtttccat acatagctcc
      181 attttttcta ggtgctataa tagcacttat gattaatcct atatcacaaa agttagaaaa
      241 taagtttcgt ataaataaag gtatctctac tcttgttcta agttttcttg cagtggctat
      301 agtcagtaca gttactacaa taatagtaat aaattcaatg aaagaattaa tgggattttt
      361 aaataatata tctgctaatc ctgaggatat aagtaatact attatgtatt ttttaaataa
      421 aataaatgac tttatgaaat catttcaaga aatagccaat tttgatgtag aacaattagt
      481 aaacaaattc agtggtgaag taatgcaaat tacaaaaaat cttcttacta gtatacttgg
      541 attggctact tctattccat atattataat atttataatt accttgttta ttgctacgta
      601 ctttatagca aaagaccttg ataagattga aaatagtttt tataatatgt ttactgttga
      661 tgttagaaag aaggtaaaaa acgtaaagaa agaggcaggg ctttcacttg ttggatatat
      721 aagggcctat acaatactta tggctataac attttttgca atctggggaa gttttgcttt
      781 atttggattg aaatacggtc taatagtagg gtttgtagga gctttaatgg atttaattcc
      841 attcctaggt ataataacaa tatatcttcc agtaattgtt tattatttcc ttataaaaaa
      901 ttattttata gctataagta tgactgtgat attttttgtt ttgtctttga ttagagaaat
      961 acttgaacct aaactagtat cagtaaatgt tggactaaat ccattagcta ccctagcagc
     1021 gatttttatt ggaatccaag taaaaggaat aattggagta attttttgtt tgggtttggt
     1081 atgtatgcat gatatactta agaaggtgga tattttttaa ttataataat tgatagattt
     1141 tttaaaaata aaattttatt cacaggtaaa taaaaaaata ttaatcttat taagaaagag
     1201 ggtatatacg tgaaacaaaa agtagtggaa agatttttaa aatatgtatc atttgataca
     1261 acatctaatt ctcagtgtga gaactgtcca tcttcagaag gtcaaagagt tttagctaag
     1321 tatatagttg aagagctaaa aacaatgggt gttgatgatg tgagtttaga tgaaaatagt
     1381 tatataatgg ctactttaaa aggaaatact gatggtgtgg atactatagg attcatatca
     1441 catttagata caattgaaga tgttagtgga aaagatatta aaccaagaat aatagaaaat
     1501 tatgatggaa aagacatagt tttaaatgaa gcgttaaatg ttattacgta tgtaaaggat
     1561 tctccagagt tggaagaatt taaaggtgac gatttaatag tcactgatgg aacaactcta
     1621 cttgggtctg atgataaagc tggtatagct gagatagtga ctgcaataga gtatctaata
     1681 aatcatccag aaattaagca tggagatata aaaataggat ttacaccaga tgaagaaatt
     1741 ggtagaggtg ctgatttatt tgatgttgaa aaatttggag caaaatatgc atatacttta
     1801 gatggaggaa tagttggaga acttgaatgt gaaaatttta atgctgcaaa tgcaaccatt
     1861 actatacatg gtagaaatgt tcatccaggt tcagctaaaa ataaaatggt aaatgctata
     1921 catatagctg ctgaaatatc agaaatgttc ccagcagatg aaagaccaga aacaacagaa
     1981 ggatatgaag gattttggca tttaaattct ataggtggaa atgtcgagaa tgtaagtatg
     2041 gcttatatta ttagagacca ttgtaaagaa aaatttgaaa atagaaaatc tattatgata
     2101 gagaatatag aaaaaataaa taagaaatac gataatagag tggaattgga tttaaaagac
     2161 tcttattaca atatgaaaga aaagattgag ccagttatgt ttatagttga tatagctaaa
     2221 gaagctatgg aggaattagg tataaaacca cgtttagttc ctgtaagagg tggtactgat
     2281 ggagcaagat tatcttttaa tggattacct tgtccaaata tatttactgg tggattaaac
     2341 ttccatggga aaaatgagtg tataccagtt agttctatgg agaaagctac taaactaatt
     2401 gtaagaatag ctgaaaaata tgctgaaaga gtttagttaa aaagtattta aaaattaagc
     2461 atttaaagtt ataatagcta tatcattctt tataagtcaa gctgattata agcttgtgtt
     2521 gtacttaaaa tggtatagct atttttgtaa ttatttttat aaaagataat tatttttagt
     2581 ttagggtatc aataaaaagt atgaatatca ataggtatta acatttaaaa gggggaattg
     2641 tattgaaaat taaatctaaa aaaataatag catttgcact ttcacttggg attatgttgc
     2701 caagttgtgt atatgcagac actattagta aaaatcaaaa tgcacaggta gatatgaaca
     2761 attatcttca aggtagaaaa tctccaaagg aggaaaaact tattggtgaa gatagatttg
     2821 atacagcgat aaaaatatct caaagtggtt ggaataatgg ttcagaaagg gtgtttctag
     2881 ttaactcaaa ttcattgcca gatgcattgg cttcaactcc tttggcaagt aaattggatg
     2941 caccaatact gcttacaaat aaaaatagta taccatataa tgtaacagag gaaataaaaa
     3001 ggcttaatcc agcagaagtt attttaattg gaagtgaagg agctatttca tcaagtgtaa
     3061 aagaatctct tgaagatatg gaagtattag tcagtagaat cggtggagca gatagagaag
     3121 aaacatcttt acttcttact agacagctag atgatactgg tgatttaggt gtctcaaaag
     3181 tggcagtggt taatgggtat aatggactgg cagatgcaac ttcaatatca agcccagctg
     3241 caagtgataa tacagctata atatatacag gtaaggatag cataagaagt gaggcaaaga
     3301 attttattac acaaaactca actaagacat acatagtagg tggggagtac agtataagta
     3361 aaaaacttga aggacagtta gtaaactcag aaagactagc agggacagat agaaaagata
     3421 ctaatgcgaa agttttagaa aagttttatg gaaaatcaag taaggttaat aatatgtatt
     3481 ttgcaaaaga tggaagtggt agagaagctg atttagttga tgggcttgct gctggagtat
     3541 tagcatcaaa aacaaagtca cctgtagtac ttgcaagtgg aagtttaagt agtgcacaga
     3601 aaagttttat aaaaaaagta aaggctgata aatttgtaca agttggtgga ggtaaaaata
     3661 gtaaaccata tgcagaagca ttagccttac agtaatattg tgtaaatcaa aattaatata
     3721 taataatagc aagtaaacca agttgcactt caa
//
LOCUS       DAELTA010000238         3675 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.243, whole genome shotgun sequence.
ACCESSION   DAELTA010000238 DAELTA010000000
VERSION     DAELTA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.243"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..610
                     /locus_tag="KPG13_003458"
     CDS             104..610
                     /locus_tag="KPG13_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG0987141.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            738..1754
                     /locus_tag="KPG13_003459"
     CDS             738..1754
                     /locus_tag="KPG13_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG0987142.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2108..2812
                     /locus_tag="KPG13_003460"
     CDS             2108..2812
                     /locus_tag="KPG13_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG0987143.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3107..3586
                     /locus_tag="KPG13_003461"
     CDS             3107..3586
                     /locus_tag="KPG13_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG0987144.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1513 a          333 c          634 g         1195 t
ORIGIN      
        1 tagtttgtaa aatcttccta tatcatataa aattaagtat tgattagtct attacataaa
       61 tatagaatat aaatatatcc atttaataat aaggagaaga gatatgaaag agtatgtagt
      121 tgtggattta gaaactacag gattagatcc atataaaggt tgtgaaataa tagaaatagg
      181 aataacagaa ataaaaaatg aacaaattgt aaaaaactat tcaaggttga taaaacctaa
      241 ggggataatt tcttcattta taacagaatt gacacatata agtaatgaaa tggtagaaaa
      301 tgaagaacca ctagaattag ttttacctag atttagaaaa tacataggtg atagaactat
      361 aattgcacac aatgcgaaat ttgatttaaa atttttaaat tactatctta gaatgttaga
      421 tttagaacca ataaacaatt atatatgtac agtagagtta ttaaaaaaat gtaaaagtta
      481 taaaggaaaa aacaagaaac ttgaaacagc ttgtgcttat tacaacatag agaatataaa
      541 tgctcataga gctgacagtg atacattagc aactgcaaaa ctatttttga aaataaaaga
      601 tgaatattaa ttataaatat agagaaaatt agtgaaaata tcctttataa aattgaccct
      661 taagcttaca attttgttct ttgatttatt ttgtagaaca atgtataatg taccttatat
      721 gaagaaggga gactaaaatg ggttattaca atgataatga taataataaa ataagacgta
      781 aaaaaattag taacagcaaa gatagtaata aaaaatctaa acattcttat actccggcaa
      841 ataaaaaaaa taaaagttct aagacaagtt ctagtaaaag aatgagtgaa aaagtagatt
      901 cacagaatct tataaaaaga gatagagata gttttacaag agataatgta agacctttaa
      961 atacagatta caatcaaaga gaaagaagaa gagtcgagta taaacaaaga gagaaaaaat
     1021 taaaaagtaa aaaaagaaaa aaacaaaaaa ggattagaat agttagagca ttattatcta
     1081 ttatgttagt tgcactaatt atattttcag tggcaggtgc aatatttaca gtttcagcta
     1141 taaaagggtc accagaagtt actaaaaagt taattaggtc taaatatata agtagtgagg
     1201 ttgtaagtat aaaacaaatg ccaaatgatt taaaacatgc aatagtttcc atagaagatg
     1261 agagatttta taagcataaa ggagtagatg ttatttctct agttaggtca gtattaaaca
     1321 atgtgtttac agatacaaca caaggtgcaa gtactataga tatgcaagtt tcaaaaaacc
     1381 tgcttactag taatgataaa actatgaagc ggaaaataag ggatatgtat aatgctaagc
     1441 agatgaataa gattatgact aaggatgaaa ttcttgaagc atatttaaat aatatgtatc
     1501 taggtaaaaa tgcatatgga gctgcaaagg gagctgaagt ctactttggt aaaaaagtta
     1561 gtgaattaaa tttagctgag tgtgctatgt tagctggtat gacaaataat cctgcaaggt
     1621 atattgacca tggagaggct aagaagagac aagtgattgt actgtataag atgcatcagc
     1681 ttggttatat aactgataag gagtatagaa tagcaaaagc tgatgacact ccatttaaat
     1741 cagaaattga ttaaaattgt aagaatatag tattttcaat agtttaaaca aacaaaaatg
     1801 taataaaaaa ggtgatttgc cacccatttg ccaccataat tataatctaa tggcaaattg
     1861 ttttaatgta caaactagat aatagaatat tgtcttaaat gctatattat gctaaaattt
     1921 aattgtattt tgtaaattaa aaaacttata atttagtttt ctagggttcc gcattttata
     1981 atggtcaggt ccaagagaaa acgcgcaatt gttgtgtaca cggaaggata aaagcctggg
     2041 agatatttaa gaatatttct ataggctttt ttatttttac atattttaaa tttgaggagg
     2101 ttttctaatg aaaaaatttg ttattgagga tgcattttgg aatctatttc ctagtgcaaa
     2161 aattggagtt gttatttgta atgatataga taactctata aaagatgaag actataagaa
     2221 aataattttg aagggagaag aagaagcatt aaaatacttg gaggactcag aatttagcaa
     2281 caaccaagtg attaaagtat ggagagaagc atttaaaaaa tttaaaacga aaaaaggagc
     2341 aaggtcttct attgaagcat tattaaaaag agttcataat ggaaataata taggaactat
     2401 taacccttta gtggatatct acaattttat ctcattaaaa tatgcattac cttgtggagg
     2461 ggaggatata gataaattta taggagatat taggctaact agagcaattg gtaatgagga
     2521 gtttatccca ctagggacag atgaaaattc atttccatat gaaggagaaa taatttataa
     2581 agatgatggt ggagcaattt gtagatgttg gaattggcgt gaagcagtga gaacaatgct
     2641 tactgaagat actaacaatg cctttttatg cattgaatta gtagatgaaa gtagatttaa
     2701 agaatttgaa aatgctttaa atgaattagc taaaatagtt caagaaaaat tgggtgggaa
     2761 ttgtaaaatt tcaattttag atattgataa taaagaggta tcagtggatt aatatggagg
     2821 tgctgttgta atggactatt ctagttataa agatacttat atttagggta agaaggtcta
     2881 ttttagatat aacgattatc aaaggatata atatagaaaa taaaaatgta gaatataaaa
     2941 agcctttaaa ataagaaaaa aatgtatttt aaaggctttt attatactct tttttatttg
     3001 cataattaac aataaaaata tattgttaat tataatattt ttattgaaaa acgatataat
     3061 ataatgtatt atactatata taaaatattt taaaggagtt ggcatgatgt ctaataaact
     3121 tataaaaaat ttactaagtg gtatattaca aattttattt tttcttttag gattagtaat
     3181 tgttgcagga ggatttaaaa gttttatgta cctctgtttt agtggagaag caactctaca
     3241 aggtactatt tatggaatat taatgtttat tttaggtgta tcatatttta tcattataaa
     3301 atcattgata gaagtattag gtagttctga atatagccta tttgtaaaag agaatgtaaa
     3361 aagatttagg attattggat atcttttgct tttaaactct ctaattgagt ttatttcaac
     3421 ttttggtact actggaaaag gaatgagatt tctagattta ggatttggat tttattttac
     3481 tgttccagta tttgtatatt ttataacttc tcttatgagt tttgtaattg cagatggatt
     3541 tgtaaaggca ataaagataa aagaggataa tgatttaact atttagatgt gacactttaa
     3601 tatataggag ataaataacc cgaaaagagt gactaactag taatttaaaa taaaatatat
     3661 tagggtataa tacat
//
LOCUS       DAELTA010000239         3666 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.244, whole genome shotgun sequence.
ACCESSION   DAELTA010000239 DAELTA010000000
VERSION     DAELTA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.244"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..508
                     /locus_tag="KPG13_003462"
     CDS             <1..508
                     /locus_tag="KPG13_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG0987145.1"
                     /translation="ARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQDLT
                     QDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQELSD
                     IENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRALKKLE
                     LYLEGGKL"
     gene            505..1266
                     /locus_tag="KPG13_003463"
     CDS             505..1266
                     /locus_tag="KPG13_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987146.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1304..2170
                     /locus_tag="KPG13_003464"
     CDS             1304..2170
                     /locus_tag="KPG13_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987147.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2191..3432
                     /locus_tag="KPG13_003465"
     CDS             2191..3432
                     /locus_tag="KPG13_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987148.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
BASE COUNT         1469 a          390 c          539 g         1268 t
ORIGIN      
        1 tgctcggatt atatcacaaa taaaagcagg aaaaaaagaa ttgtttgata ctctaataaa
       61 aaaatactat gatagcatct actgttattg ttatcgccat gtagacaata aaatgactgc
      121 acaagattta actcaagatg tatttgtaac agttataaat aatatagaaa attataaaca
      181 ttatggtaag tttgaaaact atttgtatgt tattgcaaaa aacaagtgta aggatttttg
      241 gaagaaaaaa caaccaatat atttagagtc attagataag gagatatcaa atcaagaatt
      301 atctgatata gaaaataaaa tgtttataaa agaagctttg actaaattac cagaattaga
      361 atttgaagtg ataattttac ggtattacca tgatttaaga tttaaggata ttgctaaaat
      421 tatggagtca agcgtctctg tcacaaagta caggatgagt agggcattaa aaaaactaga
      481 attatatctg gaaggaggta aattatgaat aataaagaaa taaaaagaag tctacagaag
      541 tataaggttc ccaaatatga ttcttcagct cttgaaaaga ctattaaaac tatacatgag
      601 ttaacgctta gtaatactta taaaagaatg aataataaag acttttttat atctcaaatt
      661 ggttttataa gaaaaagaac ttggattatg caatttctaa tactatgtac aatatgtata
      721 tttttattta gtacatcagc agatagtaat tcattttata atagtttatc cttgatttct
      781 atagcatcac cactttttgt actgacaaat atagaagaat taacaaaagt gtataatagt
      841 agtattttag agatagaatt ttcaacaaaa aatacattac ataaaattat tgctactaga
      901 atgcttatat ttggaataag tgatttattg tttatgatta ttattttggt atctgcaaaa
      961 caaatcattg atgtaaacat tctgtatata ataatttata tactagttcc ttttaaccta
     1021 atgtgtatag gatgtatgga aattataaat agaaataaag gtaagcatac aaattattat
     1081 tgtatgatat acggtatatc tcttgtatta ttaatagtta cactgagtaa ctttagagat
     1141 aatctatatc aatttgaagc aataaatcaa tggctaattg tttttctagt cacagcagta
     1201 acattaatat atgaaataag aaaaacgtgg agacatcttt gttcatttga aaatattatt
     1261 atctaagtaa aaaataagaa tacacagtat caggaggaat agtatggagt taagtgtaga
     1321 acgtttaaca aaacagtata acaataaaat tatagtagat aaaattgact ttaaattaaa
     1381 tgagggtata tatggatttt taggagaaaa tggagctgga aaaacaactc ttatgagaat
     1441 gatttgcgga atattaagtc ctacatctgg agaagtaaag gtggatggta aaaataattt
     1501 ggatatggga gaagatttta gagatatgat tggatacctt cctcaaaact ttggatacta
     1561 cccagatttt actgccaaag aatttatgct atatatagca tcattaaaag gattaatgcc
     1621 tagctatgca aagagaaaaa cacaagaatt attagaaatg gtaggattat cagaggtaat
     1681 taataaaaaa ataagaacat tttctggagg tatgaagcaa aggctaggat ttgctcaaac
     1741 actacttaat gaccccaaaa ttttaatatt agatgagcca acagcagggc tagaccctaa
     1801 agaacgagtt agatttcgca acctaatttc agaattttct aaggaaaaga ttgttatatt
     1861 gtctactcat attgtgtctg atgtagaata tattgcagat aaaattttaa taatgaaaaa
     1921 aggaagattt gttttaactg gctcaataga agaagtaact tcaaagataa aagataaggt
     1981 atggaaatgt gtggtcaatg agaatgaaat agattatatg tctgatagat attgtttagc
     2041 aaatatccat cattctggtg gagttgcaaa tcttagaatt gtaagtgata taaagccaca
     2101 tgacaatgca tgcaatttaa aaccaacatt agaagattta tatttatatt gtttcaacga
     2161 agtactataa atatacataa ggagataggt atgtctacat taattaaatt tgagttaaaa
     2221 aaaataataa aaagaaagtc tacaatagca gttattttag gaagtttatt cttaagcatc
     2281 attctttttg cattaatgcc aattcaggaa aaagctaatg atgaaagtgg aaaagagtat
     2341 aggggtttac aggctattga gctaaaaaag caacaatcta aattaaatga gggatacata
     2401 acagataaaa aagtaaaaaa ctcaatcaga gattaccaaa aaatgtttaa taatccaaat
     2461 aatattacaa cagatgaagg tgggttttca aattttaaac cagatattta ttggaaattc
     2521 ttctatccaa ataaagcttt ttataatttt ataatagagt catatagtaa tccaaatgat
     2581 tataatccag aaataattaa atcaatgcct ttagaagatg gagttaattt ttataaattt
     2641 agaaatgaaa aagtaaatac aatattaaat atggaatatc ctgataaaaa ttacacacag
     2701 aaagagaaag atttttggtt gtctaaaaat aaggatatag aagagcctta tttaaatgga
     2761 tattatcaag gatggaatta ttttctattg gctagagatt tactaatttt tactatagta
     2821 gctgtctgca taagtacagc ttctgtattt tcaggagaat accaatccgg tgctgattca
     2881 ataatattat cttctaaata tggaaaaaca aaagttataa cagctaaaat agtaagttcc
     2941 tttttatttg gtacgattat ttttacaata aatacaattg tgtctattgg tatacttctt
     3001 ttatcttttg gaattgatgg atggaattta ccattacaac ttacaaccag ttcaattcca
     3061 tacccatata catttttaca ggcaatatta ctaagtgtgt taattattta tatgatatta
     3121 atactattaa ttagttttac acttttatta tcatcttata tgaaatcacc ttttccagtt
     3181 ttaattacaa atataatatt actatttgta ccaatatttt taaaatatag tgaaacaaat
     3241 aagttgtata atcaaattct caatgtttta ccatcaaatg ctatttcatt ttcatttact
     3301 aattatgtag attataaatt tggtaattta ataatagctt tacctacaat gattattttt
     3361 acatgcttag ttcttacagt tgttttttta ccttttatta aaaataattt taaaaaacat
     3421 caagttaact aaaatatata ttataaaaag aaggtgtctt aaaatgaact ttttagctta
     3481 ttaggcactc ttctttgtat taatttaaat atattttagt attagattaa atatgttttc
     3541 atcattttaa caatgaaaaa gagctttatc tctattttga gatagctctt ataaagttaa
     3601 atattaaatt gataactata aaatatgtag tttgaattta taatttgtgt tataatcata
     3661 ctgatt
//
LOCUS       DAELTA010000240         3632 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.245, whole genome shotgun sequence.
ACCESSION   DAELTA010000240 DAELTA010000000
VERSION     DAELTA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.245"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            391..1920
                     /locus_tag="KPG13_003466"
     CDS             391..1920
                     /locus_tag="KPG13_003466"
                     /inference="COORDINATES: protein motif:HMM:NF012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HBG0987149.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
     gene            2468..3143
                     /locus_tag="KPG13_003467"
                     /pseudo
     CDS             2468..3143
                     /locus_tag="KPG13_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         1610 a          335 c          498 g         1189 t
ORIGIN      
        1 aataaaatga tattaaataa gatataaatg attggaaata tttactcact ttaaaattct
       61 tatggatagt agaatacaaa aaacacatga aattctatta acatcaatat tttggggaat
      121 tttaaaaaat atatggtgaa gagatagatg attttacatt ggtttaaatt acactaagtt
      181 caggtcagat aaatcagaga tataaattat atgggtgcga tgtaaataaa tatatatata
      241 cttaccttga aaaattataa tccaactaca aaagtaaata atgaaaaagt caaaaaagta
      301 gtgtgatact agcttttata aaatcaataa aacaattaaa acatcatttt aagtatatta
      361 aattgaaaaa ttataaaggg ggaatggaat atgaaaagct taaaagcatt aattgtagga
      421 gtaagtaaat atagtatagt taaaaataaa gatttactat tttgtaaaaa tgatataatt
      481 gaaattaata atgtgttaca taaaagactg gggattactt cgaataatat aagaacttta
      541 ggtatggagt atgaagtcag aaaagattta ttttttaaaa cttttcaaga aataagcgat
      601 tctataggag atgatgatac atttatattt tacttttcag ggcatggatg catatgtaat
      661 aacggagtgc attacttagt attaagtgat gaattagttt ctattcaatt tgtaattgat
      721 tttatagaag gattaaattg taaaaataaa attgtattta ttgattgttg ctttgcaggt
      781 aaatttaatg taagtgacaa acaaaaaata aatattaaag acatattttc cttgtttgat
      841 agaaaaggat gtgcagtgtt agtatcaagt agcgaatatc aagaatcatt ttctcttcca
      901 gatagttcaa ttagtagatt tacaagttta ttatcagaag cgttaagaaa tgaatacata
      961 gtaagaaaag gggagttatc tttaagagca ataagcaagt atattaattt gcgtatgagt
     1021 atattgaaca aaaattatat aaactgtaaa caaaatccta tctttaggtc taacatactg
     1081 ggagatatat attttaatgt aaaagattat aaatcttata taaaaaagaa agtttttcaa
     1141 gagcatgacg attacataat atacaatgtc aaatctttac atataagtat aataaaaaga
     1201 tattcagtag aagttatatt aaaaaataaa ttctcctatt gtgatatatc agaaatatct
     1261 aaggaggttt ttgacatagt taagtctgtt gaagtttata atagtgaaat aggagaagat
     1321 agattaaaag gaaaagatgc taatataata tgggtatatt ttggcaaaga tgaaattgat
     1381 atgataaatg gaatatatat gtgtaaaact acttggattg ataaatatca agagaaagat
     1441 atatggtata gtgtaaatgg gaaaaataaa ttttttatca acggtgttca cactgaaaca
     1501 tatgataact acgaaagctt aaaatcatat ataagtcaaa atacaatcac taaagaaaag
     1561 gcagtatacg aagttaaaga aattttaaca aatatgataa acatatctga agaaattata
     1621 tccttctata atgaatatga aaataagaaa atatgtgaag ataatatttt tcaaattata
     1681 gatagtagaa agaaaaaaat ggatgagtgt tacaataaag ttataaattt agattttcca
     1741 ccaattgaaa ttaaagattg gtttgataaa tgtataaact tagtgtcaac tatacaaaat
     1801 tttggagttt attgggatca tagtagaact gaggaaaata gaaagcaatg tatggaaatg
     1861 aatatagagg attattacaa agatttaaga gaagtcataa gattaagtga ggagatatag
     1921 aattaaattt ttccatagtt gtactaatat agaattataa gtcagattgg tactttgttg
     1981 gttacgaatt taaattaaaa taagttgttt acactaaaca tgatagaaga tacaattata
     2041 aatccattta taaattatat tggtattgat ttaaataatt agatatttat tttgaaaaag
     2101 tctagtaaat atttaaaaac gtataatatg ctgttaatgg aacttttata aaattaaaag
     2161 attatatcat ccaccccccc ccaataatac cccaactaca aaaataaaca ataaaaaata
     2221 tccgaaaaaa tcagtttaac atcataacta aatagctata tacctacact tgcaaaaaac
     2281 aacaaaacaa tcaaataacc acttatagac tcccctcgtc tccaccattg aaatccacga
     2341 atacctatat ccaattggat ataggtattt ttatacattt ttaatttaaa tattattgat
     2401 aaatacattt aaaaagtttt aacctaaagt gaagaatcta aataaaaata caatattagg
     2461 agaggttatc attacaataa cagcttgtga tgatgaaaaa gaaatacaaa tcgtaataaa
     2521 aaatcaagtt gataatatat taaaaaataa aaacaaagaa tatcgaatta aaaaaatata
     2581 gctcaaaaga agaatcatta caaagttaat tgagaggata cactactctt agatataaaa
     2641 atggcagaac taaatagtat agatatcgca agaaacataa gaaaagtaga taataatatt
     2701 tataacatct ctaataaatt atgtacagga aagatatgaa gtaagagctt attgatactt
     2761 attaaagcca ataaattttc aagaactaga aaaacacata ttaacatgta ccaaaaaaat
     2821 tgaaaaaaat aaaaataaat ctaacacata caaaatctaa tctagcaaaa aaatatatag
     2881 aagttcaaaa aaagaacatg ctaatacata caaaaaataa aactcttgat gctatatgta
     2941 tcctagaaaa aatagaaaaa gatttaaata tagaggaact tgtaagatgt cataaaagtt
     3001 atataactaa cttaaactat gtagaaaata taaagtcaaa tactgcaata ttagacagtc
     3061 aagaagaagt acctattagt agatatagat ataaagatat aaaagagggg tttctaaaat
     3121 ttattggtga tagaatatgt taaaccatat aaataaaata ttattaatat tatcattgtt
     3181 tacaccaata atgatactaa aaagtatatt aacttacgat aaagatacag ataagactaa
     3241 gaaaatatct ttttttactg tattattagc ttatcttata actaagatca ttatattatt
     3301 taatgttgat aagacccatg attataatat gaagaaaaat ctatatgata taatctatta
     3361 tttgatattt attacttggg aacttactat aattattttt ataagatagg aattgttaaa
     3421 tatatagttt catttttctt atttgacgta tatcaccctt taataaaaga tgttatatct
     3481 ataaatttat tttttgtagt tacaagtaat gtaatatcat cagaggaagc tctattagtg
     3541 tttagttatg ataatgctaa agtagatata tactattatc ttataataat agttatatag
     3601 tagcttgtgc tatatttaaa tttattaata aa
//
LOCUS       DAELTA010000241         3631 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.246, whole genome shotgun sequence.
ACCESSION   DAELTA010000241 DAELTA010000000
VERSION     DAELTA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.246"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(59..2029)
                     /gene="ftsH"
                     /locus_tag="KPG13_003468"
     CDS             complement(59..2029)
                     /gene="ftsH"
                     /locus_tag="KPG13_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG0987150.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2063..3451)
                     /gene="tilS"
                     /locus_tag="KPG13_003469"
     CDS             complement(2063..3451)
                     /gene="tilS"
                     /locus_tag="KPG13_003469"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG0987151.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
BASE COUNT         1067 a          739 c          369 g         1456 t
ORIGIN      
        1 cagttttagt atttaatcca catcagataa taatcttaaa atgaccaagg tcttaaaact
       61 atttatcttt atcagattta tcttctaaat ttttatttat atcaataaca ttgtttcttt
      121 cttcttcatc tttttctaat tttatagtta attgttcttc aacttcttta gaagaatttt
      181 cctctgtaac tgcattttca atttcattca gaggtaactc tttattaaat gcttttacaa
      241 attgttctgc atctaaagtt tcatatataa gtaaagcttg tgctacatac tctaatctat
      301 ccatattttc ttgtaatata cttcttgtct tatcatatgc accatcaact atacgctttg
      361 tttcttggtc tatctcaaaa gcaacttctt cagaataatt tctcttactt gaaaaactat
      421 tacctaggaa tacttcatca tcactgtcaa atgacattgg accaagttta gaactcattc
      481 catactttgt taccatactt cttgcagtag ctgtaactct ttctaagtca tttgaagcac
      541 ctgtagatac atccttaagt actagctctt ctgctactct accaccaagt aaaactataa
      601 tattttcttt catttcattt tttgtagcat agaacttatc ttcaacagga agttgcattg
      661 taaaccctcc agctcttcct cttggaacta ttgtaacttg atgaacagga cttacttctt
      721 caagtacatg ggcacaaact gcatgccctc cttcatggta tgctgtaagt cttctttctt
      781 tctcacttat tactttcgat tttttagcca cacctgctat aacctttgtt atagcttctt
      841 ctatagtttc catcttaatt ttcttttctc tttttctagc tgttaatatt gcagcctcat
      901 tcattaagtt ctctatatca gcaggagtaa atcctggtgt tcttctagcc aagacatcca
      961 tttttacatc atcacttaat ggtttatttc ttgagtgaac tttaaatata gcctctctac
     1021 cttttacatc tggtgtacca acaacaactt gtctgtcaaa tcttcctggt ctaagtaaag
     1081 ctgggtcaag tatatctggt ctatttgtag ctgccatgat tattatacct tgatttaccc
     1141 caaatccatc catttcaact agaagttgat taagagtttg ctctctttca tcatgacctc
     1201 caccaagacc tgcacctctt tttctaccaa ctgcatcaat ttcatctatg aatatgatag
     1261 ctggagcact tttcttagcc tgttcaaata aatctcttac tcttgaagca ccaaccccaa
     1321 caaacatctc aacgaaatca gaaccactta tactgaaaaa gggaactcct gcttctcctg
     1381 caactgctct tgatagatat gttttaccag ttccaggagg tcctaccata agcattcctt
     1441 ttggtattct tgcacccaat tcaatatatt tcttaggatt ctttaagaaa tctactacct
     1501 cctgcaaatc ttctttttcc tcatctagtc cagctacatc tttaaatgta actctagttt
     1561 tttcatcatc tttatgaacc ttagcttttg atttgccaaa actcataacc tttccgcctc
     1621 caccttgaga ctgattcata aacacgaacc aaataatcac catgaagaat attaacagta
     1681 atgtaggcaa catttcgaca aaccaaggtg tagatggctt agcttcacca ccaaaagtta
     1741 gtttcccagc cttagcttgg tctaacacct catctgcaag tttatttccc atcacttctg
     1801 ttggaacata tgacttaaac tttgtatttg tatcttttat agtaccttct acagaagttt
     1861 gatttacaaa gtataatctt gatatattct catctgttaa ttctctatat acttcagaga
     1921 attttaaatc ttttattttt tctgtaggct tacctgagaa ttgaactata cctactataa
     1981 ttataaaaac aagtagataa aaacctgccc cttttagcaa cttattcaat atagaagccc
     2041 tcctttcata cattgctttg ccctataatt tgttaaaatt aatttttaat acttcttttg
     2101 tgctttcatc aattttataa ttttcactga ttttataatc tccaacacat attattcctt
     2161 caccatctgt gattatagga attttacatc tttcttctct tgggattttt aaatctatga
     2221 ataaatcttt cactttttta cttcccatag caagctttat cttatcacct tgccttctgc
     2281 ttcttattac tataccccct tttaccttat taaaatcaaa ccctttagat gaattgtcta
     2341 atttcatact cttataccta tctatgctca ttacttgggt ctcaataatt tgttttaatt
     2401 cttttatttt aataaaccca ttactaggta cattgtaata aaattcaatt tcctcattaa
     2461 caatttcctc attcgttaat attatactgt ctttttttct ataaacaaat atatttcttg
     2521 gaagagttaa cattttattt acttttgaat tatcttctaa agacattata tcttctatat
     2581 gtctttggtc aacaaaatta gtatctccta gtatatgttt tatggaattt ctaagaactc
     2641 taactttgat agcatcatgt aagcaaacaa aatcgtctaa atttatctct acaaagcctt
     2701 tttcttttat atttgaaact tctctaaatt tagcttctgc ctctttctca atataatcgt
     2761 tatcactttt taaactattg ctcatcctca ctatagattc tattacattt gaattaaaat
     2821 tatccttcat atatggtaaa aggtctaacc ttatcttatt tctagtgtat atattttcta
     2881 aatttgtttt atctatcctc ggatttaaat tataagcttc acaatactcc tctatctcat
     2941 ttctttccac gtctaatatg ggtctaatta tgcaattatc tctaatataa tcaattccct
     3001 ttaagccttt taaacctgtt cctctcatga tacgcatcat aaccgtttcg gcttggtcat
     3061 tgagattatg acctattgct attttattgg ctttaatctt atccttaatt tcataaaaca
     3121 tttcatatct tagcttcctt gctccttctt ctaatgataa tccctcattt tcacaatatt
     3181 ttggtacatt aatagacttt acaaagaata taattcccat atcctcacaa agtttagata
     3241 catataatgc atctttttga gcttctatcc ctcttatttg atggtttaaa tgtgctgcat
     3301 atatttctat attaaaatct ttttttaatc tatttagtac atgcaataaa caaactgaat
     3361 caggtcctcc agaaagacct aaaactattt tatcgccttt ttgtattaaa ttatgtttat
     3421 ttatagtact tagtacctta tcaaatatca tatttatccc tcttacactt agttttattc
     3481 acttttaagt atactttcta taaaaaaacg ctaaaccctt gtatcaattt taagaaatta
     3541 aagtttaata taaatataat aataccaaat aaattaaata aaaggtagtt ttttcactac
     3601 ctttataaaa tttctggaaa atagccatag t
//
LOCUS       DAELTA010000242         3565 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.247, whole genome shotgun sequence.
ACCESSION   DAELTA010000242 DAELTA010000000
VERSION     DAELTA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.247"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            73..828
                     /locus_tag="KPG13_003470"
     CDS             73..828
                     /locus_tag="KPG13_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0987152.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            925..1962
                     /locus_tag="KPG13_003471"
     CDS             925..1962
                     /locus_tag="KPG13_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0987153.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2771..>3565
                     /locus_tag="KPG13_003472"
     CDS             2771..>3565
                     /locus_tag="KPG13_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987154.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGIT
                     LFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVII"
BASE COUNT         1336 a          468 c          539 g         1222 t
ORIGIN      
        1 taaatatttc actaaaaaaa aacaaatgca aagctagtac caaaaataaa tcagaaaaaa
       61 gaggtgttgt agatgttaca aattaaaaat ctttcaaaga gtttccctaa tccatatggt
      121 gaacctaata ctatctttga aaatttatca atcgatatag aagatggtga atttgtaagt
      181 atcataggta gtaatggaac tggtaaatct actttattaa atataatatc tgggcttata
      241 aaagaatctt ctggtcaaat tactttagat agtactacta taacaaattt agctgagtat
      301 aaaagaactc aaatagtaag tagagttttt caagacccaa gccttggaac ttgtccttct
      361 atgacggtta gagaaaattt atctctagca cttaataaag ggaaattatt gaacttaaaa
      421 aaatgtcttc gtcataaaac gaatgacttg gaacacttac ttgaaggaat ttctttggat
      481 ttaaaaaaat atttagatat taaagtacaa tatctttcag ggggtcaaag acaatcacta
      541 tcactaatta tgtctagctt agcaagtcct aaggtcttat tactagacga acatacagca
      601 gcacttgacc caaaaacatc aaatgaagta atagagttaa cagataaaat cgtaagagag
      661 aaaaatataa ctacacttat ggttactcac aacttgaaac acgctcttca atatggagat
      721 aggttgataa tgcttcataa gggtgaagtt gtattagatg taaaagataa agaaaaaaaa
      781 gatttaacag ttgaagaaat cttagaaaaa tttgaatatg cagtataaga aattaaaatc
      841 ttagttttaa tcagaaatat ttataatcta tcatatgaat atatgaacaa atataattat
      901 taaattttaa gggaggaaaa taaaatgata aaaagtaaaa aaatattaag tttaattata
      961 gcaggagtac ttggtgtatc aatgcttact ggatgttctc aaaatgatgg ctccaatgct
     1021 tcaaatgaaa ataaagaaac agatagcaaa aaacaaaaaa atataggtat tactcaatta
     1081 gtggaacatc catctctgga taaagcaaag aaaggattca tcaaagcact tgaagataaa
     1141 ggctataaag atggagataa tataaaaata gatttccaaa atgcacaaaa tgatatgcct
     1201 actacacaaa gtattgctag taagtttgta tccgacaaaa aagatttgat atatgctata
     1261 tctactccat ctgcacaagc agcttataat gctactaaag atattccaat aataatgact
     1321 gctgttacag accctgtaga agctggactt gttaaatctc ttgaaaaacc aggtggaaat
     1381 gtttctggta catctgatta tctttcaatt gataaaacat tagaattggt taaaacattg
     1441 actccaaaag caaagaaaat aggggttata tacaatacta gtgaagttaa ttcaaaaatc
     1501 caagttgatt ctctacatga ttatgctaag aaaaataatt atgaagtagt tgaaaaagga
     1561 atcagctctt caagtgaagt taaccaagct atttctagtt tagttggtaa aatagatgtt
     1621 ttatatgtcc ctactgacaa tttaatagtt tcttctatgc caatagtttc taaagttgct
     1681 aatgaaaaca agattcctat aatagcctct gaagaaggtt ctgtatcttc tggtgcttta
     1741 gcttgttgtg gaatagatta tgaaaaacta ggttataagg ctggagaact tgctattgaa
     1801 gtattagaag gaaaatctgt tggcgatata ccagttacta cattagatga aactgaaata
     1861 ataattaacg aagatacact aaaagcactt gacatgcaaa agttatcagc agataatata
     1921 aagtatataa agtcagatga aaatgcaaaa tctacaaaat aaaacttatt aatttcaaaa
     1981 aataggtaat atgaagtagg taatatgaaa actttatata ataagtactc ttatccaccc
     2041 taaccctttg aaaattgtgc ttattgtagc aatttgaata ttgcatataa agttattcat
     2101 tttccataaa agtcgtctat attgacgact gattatgaaa gttatataac tttctaatca
     2161 ctacttgagc tgtccaaaag aattttaata ttctccagac agcttttttt tatgatattt
     2221 gattgatata catgtctagt tattccatat ttagtaaata ttttgataaa atttccacat
     2281 tcacttataa cttaggataa cgtttttttc aaacaataca ttaatttaaa agtattctga
     2341 gtatacatta tttagtattt taattaaatt tagcagttga cttttattat tgtataattt
     2401 ataatttata acaagtcgag aatacatata attttctgac taattaaaat attaaaaggg
     2461 agagataatt atgaatactt tattaaacaa tagaaaatat tattattatt atagccacta
     2521 tttttagggt ttgtacctac gttttaacat agatacagac cccttaagac tactaaaatt
     2581 ttgggattgt atctatgttg gctatattgt ttataaagtt ttcatctaac tatataagag
     2641 ccacatagat taaactatgt ggcttattcg tgtttttacc acatagtttt gtacactatg
     2701 tggttttttt atacacaaaa ttagcaaata attaaaatta taaaaatatt ttcaaaggag
     2761 tgttttattt atgtcaggta ttatatctgt aatgacgcaa agtttaattt tatcgattat
     2821 ggcactgggg gtttatataa cttacaaaat cttagatttt ccagatatgt ctgctgatgg
     2881 tagctacaca atgggtgctt ctattgtagc attttctctt actaatggta tttctcctgt
     2941 agtagcaact ttaatggcta tcttatgtgg ttgtactgct ggtcttgtta cagggattct
     3001 tcacattaag tttaagatat ctaacttact ttctggtatt ttagttatgg gaatgctata
     3061 ctctatcaat ttaagaataa tgggaaaatc aaatattcct ctatttagct ttaaacactt
     3121 attcaatggg gaaatatctc ctatagtttt agccttagct tttgtattca tctgtaaagt
     3181 ccttttagat ttattcctaa aaacaggtct tggatacact ttaaaaggtg ttggtgataa
     3241 ctctcaaatg attaaatccc taggtataaa tatcggttct ataaaaatac taggacttat
     3301 gatttcaaac ggacttatag ctttatctgg tagtcttatg gctcagttct tgggattctc
     3361 agatgtaaat atgggtatag gaactttagt tcttggaatt gcctctatca taattggtat
     3421 tacactattt aagaaattta cttttatcaa agatactact gcaataattg ttggctcttt
     3481 tatataccaa ttcactatat actttgcaat gagtttagga atgttatcaa ctgacttaaa
     3541 actaataact gccatagtta taata
//
LOCUS       DAELTA010000243         3469 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.248, whole genome shotgun sequence.
ACCESSION   DAELTA010000243 DAELTA010000000
VERSION     DAELTA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.248"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..1587)
                     /locus_tag="KPG13_003473"
     CDS             complement(<1..1587)
                     /locus_tag="KPG13_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG0987155.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEF"
     gene            complement(1806..3407)
                     /locus_tag="KPG13_003474"
     CDS             complement(1806..3407)
                     /locus_tag="KPG13_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG0987156.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1021 a          719 c          373 g         1356 t
ORIGIN      
        1 aaattccatt gtagcattaa cttctctttg attagaaaca tacattccaa agccaaaact
       61 ataaaactct tcctttaaca tattatcatt atacttagaa gacttcttcc ataaatcaaa
      121 tacagactta gcaatctctt ttgcatcttt agatgtatat acagacttaa cattcatagg
      181 taaatatgca atattttcat cagttctcat tccaaagcca gaaaatactt cagctgcatt
      241 tttcccatct atttcatgag caaatatctt cttctcatcc atatacttag accatgcatt
      301 tgccattcca gctaaagaat ctaattctct taaacttttc ttaccttttt cttttctata
      361 tgaattaact agattataca tttcattaga taccatagcc ttaaactcat aactattagg
      421 gtcttttgta cttgtgttta taattacttt gacatttaca ttaatctcac acttttgccc
      481 aaaattatca aaagcactta catttaaaac atacgttcct gccttactgg tatccacagt
      541 tccctcaata ttaggcttca aattatttcc ataataatca gtggcaacta tattcaacat
      601 actattatca aacttatccc ctacagaaat ctctacatca ttagcagtga ttgtaggcat
      661 tgtattttta tcagatgcag aactaataca ttggtcagca accttcttat ccattccacc
      721 taaagaagta acttcatcag cacctctaag cacactctta tcacttccat cattaacaag
      781 tactataggt ttgtccttag ccaacggaga accagccaca gcatcaacta acttataccc
      841 ttgagaaaca taaaacttct ttgttccttt ataaaagcgt tgtataacct tcttattagt
      901 ttcaaatcta tctttaccag caatcctaac agaattagtc ttactcacaa gttcattact
      961 cattatctcc tctgacccaa gagaataaca ttgaacgcca tcaaccttaa atggtatact
     1021 ctttccatca gtaagtataa caggaactcc atctctagca gctacagaag atacactcat
     1081 tgcatcagcc tcacctgtgt atccatttac aagaaaaacc ttatcaccat catttactgg
     1141 tttaatagct gaaatctcct tagctataag atagctagtc tttattctat cttccccacc
     1201 aattctttta acttcaatcc ctttattctt aagttgattt tctacagatt tacctattgt
     1261 atcctctgta cctataacat aagcattctt aacatccttt agtctttttt caacatctgt
     1321 tggtatctta tctctttgca ctaacattat aggagctttt acaactccag ataatccact
     1381 tgcacttaat ccatctacta tagaattatc agtatttact aatatgactg tatcatatat
     1441 ttgtttatct gctatcaatg cagaagtctc atatctatct ttcccttgta tcttttctat
     1501 actcgaaagt gcattgatat ttggtgctcc tgccattatt aatgatattg tcaatcctat
     1561 tgataatact ctcttattta ccttcatcga actcctccta ctaatttttt tatataattc
     1621 agctaatcat aaattacacc ctcagtagtg attccctcaa aatttataaa ctactaaaac
     1681 aaaaaacacc taaaatacca taacctgtaa agcaataata aaacagatta caaaaagtac
     1741 tttagatgtg attttcattt tataatatca gtaaattaac aaaaaaacaa gatttatgga
     1801 attttttaca agctaccttc attgccattt aaaaactctt gagtagcatg cacttgacca
     1861 tcagataata tataaagccc aaatccagta ctataaaact catcactaag catgtttgca
     1921 ttgtattctg gagatttctt ccacacttca aatatagcct ttgccaagtc ttttgcatct
     1981 tgagttgttt gaacactctt agaatctata taaatatatg ctatattttc ctcacttctc
     2041 attccaaact cagaaaatac ttgtggagca tttttgccat ctatataatg ttcaaaaact
     2101 ttgttatcca tcatatactt agaccatgca tttgccatac cttctagctt actagatact
     2161 accaaaggct ctttaccttt ttcttttcta taggaattga ttaagttata catttcagta
     2221 gataccattt ttttaaattc tgggctgttg taatcatgag atttatcatc caacactttt
     2281 atttctactc tttttcctgt actttttccc cattcgtctg tagcttttaa tgtcaatatg
     2341 tatgtaccta ctctatttgt atctataaac ccatcaactt ctattggtaa taccttccca
     2401 gtgttatctt tagctactat atttaacttg ccagtgtcaa acttttcacc cttatatacc
     2461 tctgtacttc caactgtaat tgctgggggt tgtccatttg actttgaagc acttatacat
     2521 tgttgtatta ccttctcatc tatttcacct acagaggtta tattcttagc tccttctaat
     2581 acagttttat cgcttccatc atttactagt accattggag agtttttagt aagtggtgca
     2641 gctgctattg catctactaa ttggtatcca tctgatacat agaatccatc tgcactatta
     2701 aaaaagtagt ctattacgtt tttgttagtt tcaaatcggt cagttccttc tattcttact
     2761 gagtttgtat ttttaactag aggattactc attatctcct ctgaacctat acaatatgat
     2821 tgaatatttt ttacatcaaa aggcacactc tttccatctg taagtatgat tggagctcca
     2881 tctctagtag ctactgatga tacactcatt gcatctgctt ctccactata tgcattagtt
     2941 agtagtactt tatcaacttt ttttatagtt gctatttctt tagctattaa atagcttgtt
     3001 tttagtctat cttctccacc aattcttttg acttctatat ttttagaatc tagttctttt
     3061 tctactgatt tacttatagt atcttctgta cctattatat atgctttttt gatatttttt
     3121 agacatctat ttgtgtctgc tggtatctta ttttgttgtg taaatagtat tggagctttt
     3181 gtagctcccg ataatccact tgcacttaat ccatctgcaa gtgatttttc tgtatttact
     3241 aaaattactg tttcataagt ctgtttatca gctatctttg ctgctgtttc atatctatct
     3301 ttaccgtata ttttttctac tactgatgat gcatttacac ttttgaagtt tactaagatt
     3361 aatgatactg ctagacctaa tgataatatt tttttgttca ttttcatatc cccctgatga
     3421 tttttatatt ttatatttat attgtgatta ttttccattt attttctct
//
LOCUS       DAELTA010000244         3436 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.249, whole genome shotgun sequence.
ACCESSION   DAELTA010000244 DAELTA010000000
VERSION     DAELTA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.249"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            100..438
                     /locus_tag="KPG13_003475"
     CDS             100..438
                     /locus_tag="KPG13_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG0987157.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            904..1407
                     /locus_tag="KPG13_003476"
     CDS             904..1407
                     /locus_tag="KPG13_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG0987158.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            1454..2413
                     /gene="moaA"
                     /locus_tag="KPG13_003477"
     CDS             1454..2413
                     /gene="moaA"
                     /locus_tag="KPG13_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG0987159.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            2417..2887
                     /gene="moaC"
                     /locus_tag="KPG13_003478"
     CDS             2417..2887
                     /gene="moaC"
                     /locus_tag="KPG13_003478"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG0987160.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2923..3354
                     /locus_tag="KPG13_003479"
     CDS             2923..3354
                     /locus_tag="KPG13_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG0987161.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1434 a          300 c          659 g         1043 t
ORIGIN      
        1 cagattggtt aatgaaacat taaattacaa tatttgttaa aaaaattgtg agttacatga
       61 tataatgtat taaataggat actaaggagt tgatatatta tgttaatagt aactacagaa
      121 aaagtagaag gtaaaaagat atcaaaggtt ttaggattag tgagaggaag tacaataaga
      181 gcaaaacatg ttggaaaaga tataggagca agttttaaga atcttgttgg aggagaactt
      241 actggatata atgaaatgct cactgaagca agacaaattg ccataggtag aatggttgaa
      301 gatgcagaag ctaaaggtgc aaacgcagta atagcattta gactgtcttc agcttcagtt
      361 atgcaaggag cagcagaaat gcttgcttat ggaacagcag ttgttttaga agatgataat
      421 agtattcttg aaaaataaaa tataaatagt taaaaagagg catcttttaa aaattttatg
      481 gatgcctttt tttatttgaa aaaactaatt tgagaagaaa ttttaactat tatgacaata
      541 aaattagggt gatattatat aaataagtaa aattatatta tataatgtat ggtatagact
      601 ttaaaaattt tatttgtaga gaaaaatatg gacaatgtta taataattat agaaattaaa
      661 agtacttaca ttcattatgt atgtgacatg tataattagt tttatattta atacagtttt
      721 gaagtaatat cttcattttg agaacgttgg aataataaaa aatgtattat tataggtgat
      781 ataaataaaa aataaatata atatgttata ttgctgatag aaaagagaat aaaagaaaat
      841 aggttttatt aaaagagatt tttatttata ggacttaatc aatgtataga ggggtgagaa
      901 catatgttta atgttgcaat tataacactt agtgataaag gatatgaagg aaaaagagaa
      961 gatataacag gaaaaaagtt aactgaattt gttgaaaata caggagttta taaagttact
     1021 gaatatgtac ttataaaaga tgataaggaa atgctaaagg aaaatattat aagactttgt
     1081 aatagtaata aaatagattt aattcttaca aatggaggta ctggtttttc aaaaagagat
     1141 ataaccccag aagcaactaa ggaagtgttg gaaaaagaaa taccaggtct gagcgaatat
     1201 atgagaatga aatccacaga aattactaaa aaggccatac taagtagggg tgtgagtgga
     1261 ataagaaata attctataat aataaattta cctggaagtc ctaaaggtgc agttgaaaat
     1321 ctaagtttta taattgatgt tttagaccat ggcatagaag ttttaagagg agaagctaca
     1381 gaatgtgcca ctaaaaaaag tgaatagtat ataatctaga tagataaaaa atagacaaag
     1441 aggtagataa agtatgattg ataagtatgg aagaaaaata gactaccttc gtatttcact
     1501 tacagataag tgtaatttaa ggtgtgtcta ttgtatgcca ccagatgtaa aatttgataa
     1561 aaattatata aatgaaaatc taagctttga agactacaag tttattataa aggctatggc
     1621 tgaacaaggt ataacaaaaa ttagatttac tggaggagaa cctcttttat atcctaaatt
     1681 aaatgagctt ataaaattta caaaagaaga atgtggaata aataacatag gaatgaccac
     1741 taatgctatt ggtctttctg atagaatttt tgagcttgaa aaatgtggct taaataaagt
     1801 taatataagt ttagattctt tgaaggaata taagtataaa tccataacaa gaggaggaaa
     1861 cttaaaagat gtcttaaaat cgattgaatc ttgtttaaat ttaggtataa aagtaaagtt
     1921 aaattgcgtt gctataagtg ggtttaatga tgatgagctt aaagatttta tgtttttaac
     1981 agttaaatca ccaattgatg taagatttat agagcttatg ccattaggtg aggctaatag
     2041 agtttataag aagggttatt ttaatgtaaa agaaatgatt gaaaaaatat atggtttata
     2101 taaagtcaaa aatgatgaaa aaagtacagc agaatattat atgtttaaag gagctaaagg
     2161 aagaatagga attataactc cattaagctg ttcattttgt aacacttgta atcgtattag
     2221 acttacttct agtggtgcaa taaaattatg tctacattca aaagaagaaa tagatataaa
     2281 accattttta cataaaccat tgttatttag agaatctatg aaagatataa taaaacaaaa
     2341 accagaaaaa catcatttaa tagaaaataa atgtagtgat acaaataggc gtatgtatga
     2401 aattggagga taagtaatgt taacacatat aaatgaaaaa gggtatgcca agatggtgga
     2461 cataagtgaa aaagatgata ctaaaagaac ggccgtagct acagctacaa ttactcttag
     2521 taaggaagca ttagaaaaag taaaaaatgg tcagataaaa aaaggagacg tattgtctgt
     2581 agctcaagtt gcaggtataa tgggagctaa aaatacatct tcaaacatac caatgtgcca
     2641 tcagattaaa ttagttggat gtgatttaga atttgatgta gatgattcta tgtctgctat
     2701 taagatatac gcaacctgta aatctcttgg gaaaactgga gtagaaatgg aagctttaaa
     2761 tgcatgttct ttagcagcac ttacaattta tgatatgtgt aaagcagttg ataaaaaaat
     2821 ggttataagt aatatccatc ttgtggagaa gactggtgga aaatcaggag attttaagtt
     2881 cgagtaaatt aataatatat tgggttaaaa tttggaggaa atatggcgaa ggtagtgtca
     2941 ataaatataa gtgagaaaaa gggtgtaatt aaagttcctg tacaatgtgc agagctaaaa
     3001 ataaatcatg ggatagttgg agatgcacat gcaggaaact ggcatcgtca gataagctta
     3061 cttggaaaag aaagcataga taaaatgaaa atgaaaggct ttgaccaatt aaaatatgga
     3121 gattttgctg aaaatgtaac tacagaaggg atagaagttt tttccctgcc tgttggtaca
     3181 aagttaagaa tagggcaatg tgaagttgaa gtaactcaaa tagggaaaaa atgtcacaat
     3241 ggatgtgaaa ttaagaaatt gacaggtgac tgtgtgatgc cacgagaagg aatttttgta
     3301 aaggttctaa aagaaggaat tataaatgtg aatgattcaa tagatgtaat ctaatgaagt
     3361 cactattaaa atataattat aagtaaatac tattaagatt taaatatata ttttaagaga
     3421 gaattaattt taataa
//
LOCUS       DAELTA010000245         3413 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.250, whole genome shotgun sequence.
ACCESSION   DAELTA010000245 DAELTA010000000
VERSION     DAELTA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.250"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(258..434)
                     /locus_tag="KPG13_003480"
     CDS             complement(258..434)
                     /locus_tag="KPG13_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987162.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(488..584)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1381..1782
                     /locus_tag="KPG13_003481"
     CDS             1381..1782
                     /locus_tag="KPG13_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG0987163.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1779..2846
                     /gene="rsgA"
                     /locus_tag="KPG13_003482"
     CDS             1779..2846
                     /gene="rsgA"
                     /locus_tag="KPG13_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG0987164.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
BASE COUNT         1236 a          414 c          558 g         1205 t
ORIGIN      
        1 tttgttaatc taatccttca aactaaaaaa tcatttatat ttacagaatt tcattttaag
       61 agatgtttat tttcacatag ataagagaat ttaagttctc atatttatta tttgatacaa
      121 atttacatct ttattatatg tattttaaat aaaaaataaa attatgaata ctagaaatct
      181 atcttaatta gctttctaac ataaggctat ttaaagtaga taaaactaaa aagggttctc
      241 tagaaaacca aatctattta attatactta cttactaata ccatcacttg atgcaaacaa
      301 gattctacca cattttctcc gtactctagt ataaaatcat ctagttgtgt atctataaaa
      361 tcacaataat catatgaact accaatataa gcagtcaaca tatgtgtaca tctatccatt
      421 ttatctctaa acatatatat tattatcccc ctaaaaaact ttcataaaaa caaaaagaat
      481 atctcttttt ggcaactggc tgacataact catagtttat tttataagcg ttagtcccta
      541 tagctttgcg tcactaaatt tctctagttt tgccgattta gttttattct acaattacaa
      601 taatacaata gaattgctta ttattcaata tacttatttg aaaattaaat aaatttacaa
      661 atatgaacgt tattttatat caaaaaaaat agactatgca tgtagaatat atggaaaaac
      721 ttgattgtag gttttgtatg aaaagacagt tgttattaat ttaagtgaga taaattgagc
      781 aacaggcata aaatgagaaa acaaataata gcagttaatt tttattatta atactaggtg
      841 aacattaaat ttcaaaatac cactagattt tatagaataa gaaaatgaga caaacataaa
      901 tatatgaata tattaaatta tacttgaaca tacttatatt ttatgataaa atttcaataa
      961 taattattag tgaaggagtg tcttgcctga tgtcagagtt atatataatg gtaaccaaca
     1021 aataaatagt tggagacgca tatgggttct gtttaatcag aatttttaag tgtatttttg
     1081 atacatttga ttgttgtgtc ttactctgcc tttatagata actaacatta gacaacaaat
     1141 aataatatta catattactt tgttaaattg tggtgtagtg ggctttaatc tgctacactt
     1201 ttattaattt tacacagtat tattttgaat ataattatgt tgaaatagaa gatactaaat
     1261 tttctagtcc acaggggttt tctgtttttg tgtaggtaaa tatgtatacc acataaaaag
     1321 gcagatataa atacctgtgg actttttttg tagaaaatac acgatacgga ggaaataaaa
     1381 atgaattata aaaatagaaa acgagcttat tatcaggaaa attcctgtat ggataacttt
     1441 atttgcaaaa attgtggttg ccttgtagtt cctgaaggag ctggtacaca acatagaaat
     1501 cattgtccac attgtttaca tagtcttcat gtagatatca ttcctggtga ccgtgctgca
     1561 gattgtgatg ctaatatgga acctattgga gtgtgggtga gaaaaaatgg tgagtgggca
     1621 attattcatc gttgtacacg ctgtggacat cttagttcta atcgtattgc agctgatgat
     1681 aattcagtga aattaatgtc aattgcatta aaaccactgg cacagccccc tttcccacta
     1741 gaaagaatca ttgaaaagtt ggaaaaggag gaaacagaat gaatggtatg aaacaaatca
     1801 atttacaaga ctatggtcta aatagaaatt ttattactca aagtatggaa tatcatgatt
     1861 tataccttgc tcggattatc tcgcaggaaa agaactttta tcaagctgta tgtgtaaatg
     1921 gtgtactgtt tgcagaggtt tcaggaaagc tgcgttttga ggctactata caatctgatt
     1981 ttcccacagt tggtgatttc gttatgttgg atcgttgcaa taataaaaat ggtaatgcta
     2041 ttattcatca tgtgcttcat agaaaaagct tgtttgtgcg taaggctgct ggaaaggtaa
     2101 atgaggagca ggtggttgca gctaatattg acattgtttt tttgtgcatg tcactgaata
     2161 aggattttaa cattcataga ttggagaggt atctgtcttt aacatgggaa agtggagcta
     2221 tcccagtagt agtacttact aaatcagact tatgtgacaa tgtgcatcgt aaattacaag
     2281 tagttaattc tatagccttg ggtgtagatg tagttgttac ctcatctata gagccagatg
     2341 gttataaaca gatttctaag tatattaaac caggctgtac tgttgcctta cttggttctt
     2401 caggagttgg aaaatcttca ttaattaatt gtctgatggg tggagaattt ttaaatacaa
     2461 aggaaattcg catagatgat gataaaggaa gacatgccac gactagacga gagctatttt
     2521 taatttcagg tggtggtatt gttattgaca caccaggtat gagagagttg ggaatgtggg
     2581 atgtttcaga aggtttagat aaaagttttg cagatataga agcttatttc ggaaaatgtc
     2641 attttcgtaa ttgtactcac acctcagaac caggctgtgc tatctgggaa gctattgctc
     2701 atcaagaact ttcgcctaaa agatgggaat cctatcgtag gcttaaaact gaagcagtat
     2761 ttgcagacaa taaaagcgaa tacttagcaa aaaaagaaaa gaaatttaaa aatatctcaa
     2821 aaataaataa gaagattaga agataagtgt agtttttttg ttcattctat gagtctatat
     2881 actattaata tattaaattt gccatcaatt tgttacttta atgtgatttt gatggcaaat
     2941 ttggtttgtg gtattattta aagcaattta ttaatttttt taataaagtt tgatttttta
     3001 tatgatgata tatacatgtg ataatgtata catatttgtt acgataggct cctttataaa
     3061 gaggtcttat gtaagataag aaaaggcata tttatactaa tacataatag agtatatgct
     3121 tatgtatgat agagatgttt cttaagagca aaactgcaat attgctaatt tataaagtaa
     3181 aaatatatga attataaaat acaaataata tatttataaa aattattgtt ttaatttata
     3241 ataaaaattt tttgttgaaa ataatgctcg aacctataaa actattaact aatatttgta
     3301 aatgtatact tttgatgata aaatataatt aagttaagtt ggtttttata aacataaatt
     3361 tattttaagg aggtgaaata aatgaaaata tctagtcaat atagaagtca ata
//
LOCUS       DAELTA010000246         3315 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.251, whole genome shotgun sequence.
ACCESSION   DAELTA010000246 DAELTA010000000
VERSION     DAELTA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.251"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..411)
                     /locus_tag="KPG13_003483"
     CDS             complement(<1..411)
                     /locus_tag="KPG13_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0987165.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIF"
     gene            complement(559..1350)
                     /locus_tag="KPG13_003484"
     CDS             complement(559..1350)
                     /locus_tag="KPG13_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG0987166.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1386..2042)
                     /locus_tag="KPG13_003485"
     CDS             complement(1386..2042)
                     /locus_tag="KPG13_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987167.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2035..3000)
                     /locus_tag="KPG13_003486"
     CDS             complement(2035..3000)
                     /locus_tag="KPG13_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG0987168.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3106..3212)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1025 a          584 c          344 g         1362 t
ORIGIN      
        1 aaaaatattt cttataaagt aatctaatat cacaaaagca ctataaaaaa ttagtattgg
       61 gcttattctt agttttttta ctttagaaat ggttaagtat ataaaagtag acatattaat
      121 tatgcttaag ttaagcaata caatttggaa tttttgaatt tttaaattta ctactcctaa
      181 aatacttaca aaaactaata caagacaccc tactttcaat atatttataa attcatctgt
      241 tttaaagcta cttatataaa ctatagaata tccaaatatc caaagtaaaa ttattctata
      301 catatttata gaaactgtat acaaatttat atttaaaagt gcaataacca aaaataaccc
      361 attagctagt atatagacct tattcttact taaagttcct ttagattcca tattccccac
      421 cacttcttca tttttatata gttttaccta taaatatgtt accacaattc ttccaaaaaa
      481 tttgtcactt tctagttata aaaaaaaatg agtaaatatt tttaatttta ctcattttgt
      541 agattttgta tattcagttt aaaaagctgg aactatactt cctttatatt catcttgtat
      601 aaatttttta acttctttac tattcatagc ctctgataaa gccttaatct tatcactatc
      661 tttattattt tctctacaag ctattatatt tacatatgga gaatcacttg attctattac
      721 tattgcatcc ttagtaggat ttaagttagc tgttaaagca taattagaat ttattactgc
      781 tccgtcaaca tcttttaata ctgttggtaa ttgctctgca ttcatttctt ttattactat
      841 atttttagga tttttagtta tatctttttt agttataagc tctccatctt ttacttctat
      901 caatttattt tttgctaata atttaagtgc tctagctcca ttagttgcat catttggcac
      961 agctataaca gctccatctt ttaattcatc taatgactta actttttctg aatagaatcc
     1021 cattggctct atatgaactt tagatgtata agtaagttta tatcctttct ccttcacagt
     1081 ttcctctaaa aatggaatat gttgaaagaa gttagcatcc aaagaccctt catctaaagc
     1141 tgtatttgga agtacataat catcaaatat ttttacttct aaatcatatc ctttttcttt
     1201 taacaaaggt tttgcaactt ccaatatctt tgcatgtgga tttgatgaag ctcctactag
     1261 tattttttta tcctctttgt ttgaacaacc tactgctgat attgctattg ctgacaccaa
     1321 tgctactgat aataatttct ttaatttcat ttttactccc cctctttaaa atgtttataa
     1381 ttaatttatt ttagtttctt ataagccaga tttcctactc cctggattat ctgaactaat
     1441 attataagtg ctattacagt atataccatt actgctgtat caaatctttg atatccataa
     1501 ataagtgcta tattacctaa tcctccagct cctactgcac ctgccatagc tgtataacca
     1561 agtatagata ttattgataa tgtaattcca gatactattg aaggcatagc ttcctttatc
     1621 ataactttaa atactatttg tctttttgta gctccaaaag attttgctgc ttctatcaat
     1681 cctttatcaa cttcatttaa tgagctttct ataattctag cgataaacgg tgctgcccca
     1741 atagttattg gcactattgc agcagtttcc cctatacttg ttccaacgat taatctagtt
     1801 attggaatca ttgcaactat aagtatcata aatgggaaac ttctaaatat atttactata
     1861 aatcctaatg cactatttat tgtgctattt ggttttaatc catctggttt tgtaactact
     1921 aatatgattg ctaatataaa tcctaatatt gttgccacta tagttggtac tataaccata
     1981 tacaaagttt gtaacaatgc atttggaaat agtgttgtta aaaagtcaat taaactattc
     2041 atttatcatt tcctcccatc ttatgccctt actagttaga tattgtttta cttgctctcc
     2101 acttttatct gaaatgttta taataagtga acctaaaata tcatctttaa attgttctaa
     2161 tttcccaaat attatagaca catctatatt tagttctctt gccatagtcg taataatagc
     2221 ttcattagaa atatctttag ggaatagtat ctttatattt gttccttttg gcaatattat
     2281 actttcttca cctattaatt ttctaagtcc tttagtattt cttaagaata tttcttctgt
     2341 atctcctacc tctaatactt ctccattttc cattattgct actcttccac aaatctgctt
     2401 tataacttcc atttgatgag ttacaactat tattgttatt cccaattttt tatttatatc
     2461 ttcaagcaat gaaagaattg actttgtagt atttgggtct aatgctgaag tcgcttcatc
     2521 acaaagaagt acttgtggat ttagtgctaa agctctagca attgcaaccc tttgtttttg
     2581 tccgccactt agatttctgg gtttatcatt tttcttttca gatattccaa ctacttctaa
     2641 taactctaaa actctttttt taatttctgc ctttgagtat ccaaaacatt ctaatggcaa
     2701 tgctacatta tcaaatacag tttttctttc aagcaatgaa aagtgttgaa aaatcattcc
     2761 taactctttt cttaaatctc tcatttgctt ttcattcaat gactttactt ctttatcaga
     2821 aacaagtaca ctgccttctt ggtattcttc taatccgttt atacatctca gtagtgttga
     2881 tttccctgca ccactatgac ctattattcc aaaaatttct ccagattcta tttcaatact
     2941 tacatcttta agcacttgaa tctttccata atacttatta acatttttta tgctaatcat
     3001 aaaatttcct cccatatatt ctgctcagtt tttatgttta tcctctctta tgtattacaa
     3061 caaaataaaa gcccgagtat aaactcaggc ttagaaagct atttatactc atctatcaac
     3121 tatattagtt gcaggattta gcaccaatgt atataaaata tactggttgc cgggtttcaa
     3181 agggccagtc cctccaccac tcttgataag acgttattta atttaattct attatcattt
     3241 gttgctatgt tatatatgat atattcatta atagtatttg tcaataaatt tatttaaatt
     3301 ttttattttt tatta
//
LOCUS       DAELTA010000247         3286 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.252, whole genome shotgun sequence.
ACCESSION   DAELTA010000247 DAELTA010000000
VERSION     DAELTA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.252"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            370..2721
                     /locus_tag="KPG13_003487"
     CDS             370..2721
                     /locus_tag="KPG13_003487"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG0987169.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            2743..3282
                     /gene="nrdG"
                     /locus_tag="KPG13_003488"
     CDS             2743..3282
                     /gene="nrdG"
                     /locus_tag="KPG13_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG0987170.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         1350 a          354 c          606 g          976 t
ORIGIN      
        1 atgcctcctt ttaaattatt atttatatta tacgacaaat ttaaagaatt tacataagga
       61 aatttgtaga tatatacatt aatatatata atagcctaca ctttttgcct aaaattagtg
      121 taaaaaaaag agaaaaaaat tttaactttc aaagttgtta gaaacgctac tgttcaaaat
      181 atccacatag tatttgcaat gatttgcgca agtttgacta ttatgtacaa atttggtaat
      241 cttaatatag caattattaa gataatccct ttaacttagt tgaaggggat ttttttagac
      301 ttaacatact acatatagtg gtatggggat taataaaata caatatatag taacaggagg
      361 tttttttaat tggttgaatt cgtaaaaaaa agagatggaa gagtaattcc atttaatgaa
      421 gatagaataa caagagctat atttttagca gcaacgaatg ttgcagaaag agaaggaata
      481 gtacctgatt ataaattatc tgagcaatta actcaagaag taataaagtt tttaaatcat
      541 aaatactcag aatcagttcc aagtgttgaa gatatacaag attctgtagt taaggttctt
      601 atagaaactg gccatgcaaa aactagtgaa gaatatataa tatacagaac tgaaagaagt
      661 agaataagaa attcaaaaac aagattgatg aaggcaatag aggaaattac ctttgaagat
      721 gcagaagatg ctgatataaa aagagaaaat gcaaatatta atggtaatac agcaatggga
      781 actatgttac aatatggaag cactgtatct aaagaatttt gtaaaacaca tatattaaaa
      841 ccagagcatt cttttgctca tgataatgga gatatacaca tacatgatat ggatttttta
      901 aatatgggaa cattaacttg ttgtcaaata gatgttaaaa aactattcaa tggtggattt
      961 tcaacaggac atggcttttt aagagagcca caagatataa taagttatgg tgcattagct
     1021 gcaattgcta tacaaagcaa tcaaaacgat caacatggag gtcaaagtat accatttttc
     1081 gattatggat tggctgaagg tgtatataag acatttaaga aattctacat aggaaattta
     1141 gctaaagcat tgaagttatt taaaggaatt gaaaataatg atgtgataaa gaacatagta
     1201 tataatacag agaaagaaac aaatcaaaaa gttggtctta agagagatga attgtatcta
     1261 aacttagaaa aagagaaatt aatacagact tttgatatag atgatgaact tgtaaataag
     1321 atgcaaaact ttgcatttga agaatcgtat agagaaactg ataaaaaaac atatcaatct
     1381 atggaagcat ttatacacaa tctaaataca atgcactcta gagcaggtgc acaggtacca
     1441 ttttcaagtg taaacttcgg aacagatact tctgaagaag gaagaatggt aactaagaat
     1501 cttctattat ctcaagaaag aggtttaggt aatggagaga caccaatttt tccaatttta
     1561 atatttaagg tcaaagaagg cataaattta aatcctgaag accctaatta tgatttattt
     1621 aaattatcat gcagagtatc agctaaaaga ctattcccta attttagctt tttagatgcg
     1681 ccttttaatg caaaatatta taaaaaaggt gaaccagata cagaggctac atatatgggt
     1741 tgtagaacaa gagtacttag taatgtgtgt ggttcagaaa cagttagtgg tagaggaaac
     1801 atatctttta ctacagtaaa tttaccaaga ttgggtataa aacatggtat tataaataat
     1861 gaaaaggcta atttagatgg tttctttgaa gaattagatg agaagataaa cttaattata
     1921 gaacaattat tggagcgcct tgaggtacaa ggaaataaaa agatgaagaa ttttccattc
     1981 ttaatggggc aaggtgtatg gaaaggttct gatgatttag gaccagaaga tacactaaaa
     2041 gaagtaataa aacaaggaac attgacaatt ggatttatag gtttagcaga gtgccttatt
     2101 gctcttatag gtaaacatca tggagaaagt aaagaagctc aagaattagg attaaaaata
     2161 gtgtcacata tgagacataa aatggatgaa gcaacggaca aatataaatt aaatttctcc
     2221 ttaatgggta ctccagcaga aggtctttca ggtaggttta ctaaaataga taaaaaagtt
     2281 tatggagaaa ttaaaggtat cacagataaa gaatattata ctaattcatt ccatgtacca
     2341 gtttattaca atataagtgc atatgataaa attgagatag aagctccata ccatgaattg
     2401 actaatgcag gtcatataac atatgtagag ttagatggag atccatctga taatttagaa
     2461 gcttttgaaa ctgttataaa agctatgaaa gatttgggta taggatatgg aagtataaat
     2521 catcctgtag atagagaccc aatatgcggt ttttcaggtg taataacaag taatatatgc
     2581 cctgtatgtg gaagaaatga agatgaaagt gacatcaaat tcgaaagaat aagaagaata
     2641 acaggatact tagttggtac agttgataga tttaataatg ctaaaaaagc agaagtaaga
     2701 gatagagtaa aacatagata gttttatttg ggggaataag ttatgaaaat aagaatgtca
     2761 tctactataa gttatgacag catagttgat ggaccaggat taagaatggt aatatggact
     2821 caaggatgta ttcataattg taaagagtgc cataatcctc agacacatga tttatgtgga
     2881 ggattttata tggatacaga agaaattata aataaagtca aatcattgaa attacaaaaa
     2941 ggcattacgc tatcaggagg agaaccattt ttacaaccag aaccattaga agaaatagca
     3001 agagaagcta aaataaatgg gttagatgta tggtcatata ctggatttac gtttgaacaa
     3061 ctgttagata aaaaaaatag agcgtatttt aaaaatttaa atttactaaa gcaaattgat
     3121 attttagtgg atggaaaatt tatagctgaa aaaaaagata taagcttaaa gtttagaggg
     3181 tcatcaaatc aaaggataat tgatgttcaa aaaagtttga aatataaaaa agtgtttttg
     3241 gttgagcaat atatgaagga tgatttatct atagcagaat aaaata
//
LOCUS       DAELTA010000248         3263 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.253, whole genome shotgun sequence.
ACCESSION   DAELTA010000248 DAELTA010000000
VERSION     DAELTA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.253"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..630
                     /locus_tag="KPG13_003489"
     CDS             <1..630
                     /locus_tag="KPG13_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987171.1"
                     /translation="GLQNGEQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNF
                     IIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNS
                     ITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGEL
                     KGFKGIIDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            996..2177
                     /locus_tag="KPG13_003490"
     CDS             996..2177
                     /locus_tag="KPG13_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0987172.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2319..3236)
                     /locus_tag="KPG13_003491"
     CDS             complement(2319..3236)
                     /locus_tag="KPG13_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987173.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         1273 a          373 c          533 g         1084 t
ORIGIN      
        1 ggattacaga atggagaaca acaaaatatc aatgaaatta aattttctat tggaagtaaa
       61 ttatacttag aaatagtaaa taataaaagt aaaaatggat atattgaagg acatgatagt
      121 tactctaact ttattatata tgatatagaa gataacactt ttaatataag taataaattt
      181 atggataatc taaacaaaga aaagaatgca aatccaaagg agattggctt attaagtaaa
      241 actaagctag attatatggt aaaaaacaat aaattgaatc ttatagttac ggatatgagt
      301 aattccataa ctaatcttgt ttatgatatt aattctaatg agattaaatt agaagattat
      361 gagatacttg atttaaaagt agattataat gaagcatcat ctggagttag gaaagttaag
      421 ctactggatg ataagatata ttctatttca ggtattaaga acagtatatc aggagaatta
      481 aaaggattta aagggattat tgatgtagtc ggaactaagc cagtaaaatt tcaagtattt
      541 gatatgaaac ttaaaaagaa tatttatgaa gctgagctaa taaatggaga tataagttta
      601 caagaaaagt tattttttgt tgcaaattaa gtgttagaat taattttaga actttaaact
      661 tttatatgta tcacagataa gtataagtaa gggagtgtct caaaataaac tttttagctc
      721 atgagacact ttttttatat gaaatcaaat atattttcat tgtttagatt tattttgata
      781 atgaaaaaaa gattatcttt attttaagaa agacatttat ttataattat atacaaaaat
      841 attttttaat gtttttgtgt ataattataa caatttaatt tatattaatg cacttaaatt
      901 tgaaatagaa tgcttatagt ggtaaaatta attattagaa tattagaaat aataaataaa
      961 taatacattg tacttaaaag ggggagaaat ttaagatgaa caaaaaatat attaaagcag
     1021 ggaatttaat tgatggaacc ggaaatagta ttatgcaaaa taagggtata attattgatg
     1081 gagatactat agttgaaatt gaagatataa gagatgattt aaatggatat gatgtgtacg
     1141 attacttaga taaaactgtt atgccaggca ttatgaattg tcatgttcac ttgacaatgg
     1201 agccagttgg aaatccaaag atatattatg ataatgtatc agatgtagag cttgtagtaa
     1261 atacgataaa acaacttgat gcttatttgg attcaggagt aacatatatt agaagcttag
     1321 gttgtcccaa atatgtggat gtacaactta aaaatcttat agacaaaggg tctatagatg
     1381 gtcctggtat agttacttca ggtccagtta tatgtatgac aggagggcat ggatattatt
     1441 ttggtattga aagtgatgga gtagatgaat gtagaaaatc agccagaaca gttttaaaaa
     1501 atggagtaga ttgtgtaaag ataatggcaa ctggaggtgt aacaactgat ggagttgagc
     1561 cagggtcacc acaattaact ttggaggaaa tgaaggctgc tgtagatgaa gctataaaag
     1621 caggtaaaat aactgccaca catgcacaag gaagaactgg tataaaaaat gcagtgcttg
     1681 caggaattac ttctatagaa catggtgttt atttagatga tgaaataatt gatttgatgc
     1741 ttgaaagagg aacttactta gttccaactg tggctgcacc atattttatt ctaaatgcag
     1801 gaaaagaaag tggaataact gaagctactc ttagaaagtg tgagattgtt gccattccac
     1861 acaaagagtc attcctaaaa gcatataaaa aaggaattaa aattgcagtt ggttctgatg
     1921 caggaacttc ttataatgaa catggaaaaa catattatga aatgaaacta atgtcagatt
     1981 atggtatgga taatatggat ataatagttg ctgctacaaa aacagcatct gaacttttaa
     2041 gaattgataa aaattatgga actctagaaa aaggaaagaa agcagatgtt atagttgtaa
     2101 atggaaatcc agtagagaat atagacgtac ttgcagatgt gttagcagta tttaaacttg
     2161 gtaaaaaagt tagataaata gtataaaaat aaaaaagagt gtctaaagaa atttttaaat
     2221 aattatagac actctttttt attagtaata attaaatctt ttatacttta gaaaatagat
     2281 tctattttat atttagatat aactctttaa aatcattctt atgcaatata atctatagaa
     2341 tctacacata caaagtatat tcttgaatca ttagaatttc caagtactat aacatcattt
     2401 ttcttaccta gaacttcaac accagttaga accaaactac cagctactac agtaacttga
     2461 ttatttatat ttgacatata aagattatct aggatacctt tattacaaca gcaatcatca
     2521 caatcgcagt tgcaattaca ttctttgtaa tgttttttat caagatagta acctaaagat
     2581 gctatgaaat cttcataatc tgcaggtgta atatcaaatg caacagcttt aagattacac
     2641 aatgatgcta aatctacgtt tgctaatgga gtagatgcag caagtataaa atcaattata
     2701 gcagcaagta ttgcatctaa tatagcccca aggtctatcg taggaggaat aactgcatca
     2761 ataagtgcaa ttaattcagc tactcctggt atagttccta tagtattaat taatccctca
     2821 agagttaaag ggactggaat aggataagat actctaccag ctatatctat taaatcacag
     2881 ttacaagcag aaaatctttc aaaagtacca tcaagtccag ataaattatc ttttggagga
     2941 gctgaaagat ctatacctac caaattagca cctactataa agaaatctga gataaaagca
     3001 aattgattaa agtttacaaa aggtcttaga gcatcatatc ttagaagttc taatgcttct
     3061 ctcatacttt ttttacaaca acattgtttt attttaggtt ctttatattt atttggataa
     3121 ttatagtatc tatcagctac ttgataattt tcatagtatc tttcatttgt atttggataa
     3181 tcttcctcat attcttgtgt aaatctaaag tcctctctac atttattatt ttccactaaa
     3241 tacactcctt ttatatttta att
//
LOCUS       DAELTA010000249         3258 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.254, whole genome shotgun sequence.
ACCESSION   DAELTA010000249 DAELTA010000000
VERSION     DAELTA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.254"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..905)
                     /locus_tag="KPG13_003492"
     CDS             complement(<1..905)
                     /locus_tag="KPG13_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987174.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKG"
     gene            complement(910..1647)
                     /gene="cas6"
                     /locus_tag="KPG13_003493"
     CDS             complement(910..1647)
                     /gene="cas6"
                     /locus_tag="KPG13_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG0987175.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1905..3014
                     /gene="ugpC"
                     /locus_tag="KPG13_003494"
     CDS             1905..3014
                     /gene="ugpC"
                     /locus_tag="KPG13_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG0987176.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            3044..>3258
                     /locus_tag="KPG13_003495"
     CDS             3044..>3258
                     /locus_tag="KPG13_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBG0987177.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLK"
BASE COUNT         1178 a          473 c          353 g         1254 t
ORIGIN      
        1 atacctttat tcgtatcaat atcaaagcta tatagtctaa ttgaacttat tttttcatgc
       61 aaaagttttt tataaaatga tttatctgaa tacatttttc ttttaagcat tatcttatga
      121 tttaaaaatg ctaattctct taaatcattc gtatatgcaa aaaccattgg tctttttaaa
      181 tttatatata aaagacacat taagttgaaa aattcacact caaaacaaat attactttca
      241 acttgtccac ttcttttaaa gcctgatttt tctgcacctc taaataaaaa gttatactta
      301 gagtcctttc gttctcttaa atcaatatct agttttgttg ttttatttat attgcaaaca
      361 ttacatacac tatctttttc ttcatattgt gtcaaatcaa gtttatctat ttcatctatc
      421 tctatatttg atttaattac agtatctact attcttttta catttgtttc catattttct
      481 aaactcgtgt ttaatccata cttaccacta tttctaacat atggaaacct cctgttttta
      541 aaatcttttt taatatcttc cttttgttta gcagttgcag aagatttatc taattcttca
      601 ataaaattta catacgttaa attatctata ttactaaata ttttttctaa ggtttccttt
      661 ttcccatttt taaatacctt aaaaagtcga ttttctaata tataatcaga tatatactta
      721 gggtctatat attctgcaga ttctaaagat aaataatttc tatttaactc acaagtaaat
      781 tttagcttat aatcattttt ttctagttcg ttttttaaat ccattagtaa ttcataaaag
      841 taaacaatgc caatatctct tacaatatca cctgatctat aaaatttagc ttttatttct
      901 ctcacctcgc tactttaata tatcaaccat tcctgcattt tctgtccttc tatatccaat
      961 tcccatttta tacagtgcat ttaaatcttc catatctcct tttaaaaaaa atgttcctgc
     1021 ataagcattt atatagtaat attctctcac tttaaatcca cgaatctttt cttttaacac
     1081 tctttttttg aagttaatag gtataaattc caatggcttt ctaagcccac tacctcttat
     1141 actctccaga gttagattag ctatatagtt taaacattct atataatttg aatcttctac
     1201 atctaaatat ttaccttctt tattctttac tattatagga gacatagttt taaatataac
     1261 accattttct tttatctcgt gttctttatg tatttttata ttttttattt taaatctata
     1321 acctttatat tctagctcct ttacttttaa aattccattg tataatttac tgaacataga
     1381 ataattataa aaacttatat tgaatattat atctccatca acaagtaact catttttgct
     1441 tcttataaga tttggtatgt aaatggctgt atttatatcc tttatttgct tattgttttt
     1501 tctttcttcc ccatagttaa atatgttttt agcaaaatct ttgttttctg catttaaaat
     1561 attttttata aaggaaactg acaacattga attttcattt ggaaatttgc cattacatga
     1621 ttccataaaa attgaaattc tgctcatatt ttcacctcct actttctagt ataattcatt
     1681 attctctatt tgtaatataa aaataaaaca tatcataaaa tgattttttg ataataaatt
     1741 taattttgtt ttttaatttt ttaaatatat tctactgtta gttttattct ttgctaaaaa
     1801 ctaaatatac atattaaaca aatacattta gtcattttac acatttaata taataaaaaa
     1861 atttgatact atataattaa aatttaaata tcaaggagga tattatgtca gaagtaattt
     1921 taaaaaatat atcaaaactt tactcaaatg gatttaacgc agtaaaaaat ataaatatag
     1981 acattaaaga taaagaattt atcgttttag taggaccatc tggttgtggt aaatcgacta
     2041 cacttagaat gattgctggt cttgaagaaa tctctgaagg agaactatac attggagata
     2101 aacttgtcaa taatatagaa ccaaaagata gagatatagc tatggttttt caaaactatg
     2161 ctttatatcc ccacttatct gtttacgaaa acatggcatt tgcacttaaa cttagaaatt
     2221 tgcctaaaga tgaaatagac aaaaaagtta aagaagctgc taaaatatta gacttactac
     2281 ctcttttaaa taaaaaacct aaaacattat ctggaggaca aagacaaagg gttgccttag
     2341 gacgtgcaat agttagaaat cctaaggtat tcttaatgga tgaacctcta tccaacttag
     2401 atgcaaaact tagaactgct atgagaacag agataactaa gctacaccaa cagcttggaa
     2461 ctacatttat ctatgtaaca cacgaccaag ttgaagctat gactatggct gatagaatcg
     2521 tagtaatgaa ggatggagta gttcagcaga ttgcaactcc acaagatgta tacgattatc
     2581 cagctaatat atttgttgca ggatttattg gagcaccaca gatgaatttt attgatgtaa
     2641 tattaataga agaaaatgat gaaatttatg cacaaaatga acatattaag cttaaattga
     2701 ataaagaaaa acactatgat ttaataaaag acaattatat taataatgaa gttgttatag
     2761 gtatcagacc agaagatatt catgtagaag atacttttat taaatctaat ccagatactt
     2821 gttttaaatc aagaattgaa ataaaagaac ttatgggagc agaaacttat gctcacttaa
     2881 aactaggcga aaatacgata acaattagat ttgacagtaa aaacagaatt aatgttggtg
     2941 atgacttaac actttcagtt gacaatagta gagtacacat atttgacaaa gaaactactt
     3001 tagctataag ataagaatat aatcttattt ttaaggagac tttatggaag agttaaaaga
     3061 ttttttagaa agacagctta ataaaaaaat aaatatctat ccatatgaaa accaaaagtt
     3121 agatgctagt agccatttag ttactttcaa taatgctatt tatgttattg attttctaga
     3181 taaaaataat ctcgataatt taaaaggcaa cttttattta atttaccaat ctcatataga
     3241 ctttgaatat ctaaaaaa
//
LOCUS       DAELTA010000250         3249 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.255, whole genome shotgun sequence.
ACCESSION   DAELTA010000250 DAELTA010000000
VERSION     DAELTA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.255"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(56..628)
                     /locus_tag="KPG13_003496"
     CDS             complement(56..628)
                     /locus_tag="KPG13_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG0987178.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(657..923)
                     /locus_tag="KPG13_003497"
     CDS             complement(657..923)
                     /locus_tag="KPG13_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG0987179.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(923..1132)
                     /locus_tag="KPG13_003498"
     CDS             complement(923..1132)
                     /locus_tag="KPG13_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG0987180.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1401..2261
                     /locus_tag="KPG13_003499"
     CDS             1401..2261
                     /locus_tag="KPG13_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG0987181.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2300..>3249
                     /locus_tag="KPG13_003500"
     CDS             2300..>3249
                     /locus_tag="KPG13_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG0987182.1"
                     /translation="MFILKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNI
                     DKFKSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYT
                     FGNLDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLE
                     FVNLEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCF
                     NKFKESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKL
                     KDIEEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIK"
BASE COUNT         1246 a          378 c          535 g         1090 t
ORIGIN      
        1 aacacaacag taaattataa attatcaaat aataatagtt atacaaataa aaattttaga
       61 acattttttg agattcttct aactcgtaag catccattaa agcttcacca aatctttgat
      121 agtgaattat ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg
      181 ttaggtttat aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat
      241 gtaaatcggt tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat
      301 ttgaatctgt tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag
      361 cttttaactc ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct
      421 caacgtgagc tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag
      481 gcattgtata tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac
      541 caaattgagt tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt
      601 gttgtatagt tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa
      661 taattaaatt ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt
      721 ggagcatacc caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc
      781 gcaaattgat tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg
      841 tctaaatata agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt
      901 tcagctctag atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat
      961 ataattcttt gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa
     1021 attttaatgc atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc
     1081 tagccaaaga tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa
     1141 cctccttgtt gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa
     1201 atcgttacta ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg
     1261 ttatttatgt tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta
     1321 aagtaaaaat tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca
     1381 taaatatacg aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc
     1441 cttttgagga aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagaa
     1501 aaaatttatg gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg
     1561 ataagatagc tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc
     1621 aagagttaaa aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg
     1681 gatatcatgc aaatataaga caattactta tagataattg tgagtatcta aatataccaa
     1741 tacatttatt tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat
     1801 gctatatgtg tgcaagaatg aggagaggag cattatattc aaaggcagaa gaactagggt
     1861 gcaacaaact tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaact
     1921 tattgtgtgc tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag
     1981 gtataaaaat tataagacca ctttattata taagagaaga acatattata agatttattc
     2041 aaaatagtgg tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta
     2101 ataaaagata tgagataaaa gacttaataa agagtttaga atcaaatttt aaaaatgtag
     2161 aaaaatctat atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa
     2221 aagatgggga gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca
     2281 ctataaacaa ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa
     2341 tatgccaaat tctaaatcaa aaagagcagt atttaattct gatataattg caattggagc
     2401 agttgtgtat gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt
     2461 aagtaatgag gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt
     2521 gaagagtgca ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt
     2581 ttctgatatt aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac
     2641 agatatgaga agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt
     2701 atttgtagat ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc
     2761 attgaaaaat ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt
     2821 aaatgatgca tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca
     2881 aaatctttta ataatacgtg acataataag accaccattt aacgatataa attctaattt
     2941 agataattgt tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat
     3001 tgtagatttt tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga
     3061 tgtaaatata caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga
     3121 taaattgaaa gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgacat
     3181 gaaagatgta ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga
     3241 gataaaaaa
//
LOCUS       DAELTA010000251         3163 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.256, whole genome shotgun sequence.
ACCESSION   DAELTA010000251 DAELTA010000000
VERSION     DAELTA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.256"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     misc_binding    2..258
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            334..3000
                     /locus_tag="KPG13_003501"
     CDS             334..3000
                     /locus_tag="KPG13_003501"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG0987183.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         1254 a          354 c          617 g          938 t
ORIGIN      
        1 gactgagaag aagaggagta agtaaatact actaaaagag aggaaaattc actggctgta
       61 agattttcta agtataagat ttactgaatg tagctttgga gtttctaaac tgaactataa
      121 gtaagtttag acggttagaa ccgttaaatt ctttgagagt caaagttgat aaaataacat
      181 taaccaataa tttaatgata tgttaatgta ttttaagctt tggaattaag gtggtaacgc
      241 gagcttttcg tcctttttaa agaggatgaa gagctctttt ttatttctaa agatatttaa
      301 aaaatttatt tataaaatac aggaggcagg acaatggaaa atacgaattt atcaaaaact
      361 tataacccaa aagattttga ggctaggcta tataaaaaat ggatggatga agggtatttt
      421 aaatctaaac caaatccaga taaaaaacca tttacaataa tgatgccacc accaaatata
      481 acagggcaat tgcatatggg acacgctctt gaccatacac ttcaagatat attaatcaga
      541 tggaaacgta tggatggata tgaagctttt tggcttccag gaactgacca tgcttctata
      601 gcaacagaag ttaaagttgt tgaaagaata aaaaaacaag aaggcaagac aaagtatgaa
      661 ataggtagag aagaattttt aaagagagcc tgggaatgga aagatgaatt tggaggaaaa
      721 atatctaatc agcttaaaca actaggtgat tcttgtgatt gggataaaga aagatttact
      781 atggatgaag ggtgtaatga agcagttata gaattttttg taagcctata tgaaaaaggt
      841 cacatataca gaggaaatag aataataaac tggtgcccag attgcaaaac tactttatca
      901 gatgcagaag ttgaacatga agaacacgac ggaaactttt atcatataaa gtacccacta
      961 aaagatagtg aagacttttt agaaatagct acaactagac ctgagacaat gataggagat
     1021 actggtatag cagtaaatcc agaagatgat agatataaac atttaatagg aaaaacagca
     1081 atacttccat tagtaggaag agaacttcca atagttgcag atagttatgt tgatttagag
     1141 tttggaactg gtgcagttaa gatgactcca gcacatgacc ctaatgattt tgaagtagga
     1201 ttaagacata atcttgagca gttaaatact atgaatgaag atggaactat gaatgaagtt
     1261 tgtggaaaat atgaaggtat ggatagattt gagtgtagaa aagctatcgt agcagactta
     1321 aaagaacaag gttacctaat taagataaaa gagcataatc ataatgtagg aacttgttat
     1381 agatgccata cagtagttga gcctagatta tcagagcaat ggtttgttaa gatggaagaa
     1441 cttgcaaaac cagctataga tattcttaaa aaaggagaat tagaatttgt tccagataaa
     1501 tttgataaaa cttatttaca atggctagaa aatataagag attggtgtat atcaagacaa
     1561 ttatggtggg gtcatcaaat accagcttat tattgtcaag agtgtggaga aatagtagtt
     1621 gctagaaaaa tgccagataa gtgtcctaaa tgtggaagca ctcattttaa acaagatgaa
     1681 gatgccttag atacttggtt cagttctgca ttatggcctt tctctactct aggatggcca
     1741 aataagactg aagctttaga ttattattat ccaacaagtg tacttgttac aggatacgat
     1801 ataatattct tctgggtagt tagaatggca tttgcaggta tgttctgtat gaatgaaaag
     1861 ccatttgacc atgttttggt tcatggatta gtaagagatt ctcaaggtag aaaaatgagt
     1921 aaatctttag gaaatggtat cgacccattg gagataatcg agcaatatgg agctgatgcc
     1981 ctaagattta cattaacaac tggaaattcc ccaggaaatg atatgagatt ctatatggaa
     2041 agagtagaat ttgctagaaa ctttgcaaat aagttgtgga atgcatcaag atttgtattt
     2101 atgaatattg atgaagatat aattaaaaac atgactagag aaagtgttaa agaggactta
     2161 actctagctg ataaatggat aatatcaaga gcaaataata ttgtaaaaga agcaactaat
     2221 aacatggata agtttgattt aggaatagca cttcaaaaga tatacgattt tacttggtca
     2281 gaatactgtg actggtatat tgaaatggtt aaacctagat tatatggaga agatgcaaat
     2341 gctaaatctg cagcattata tacattaact tatgttcttg aaaaaatatt aaaattatta
     2401 catccataca tgccatttat aacagaggaa atatatactc atcttcctac tgttgaagga
     2461 tgtataatag tatctgaatg gccaaaatat aatgaagaag ataacatggc tgaagaagaa
     2521 gatatgatga acttattgat ggaaggtata agaagtataa gaaatgtgag agcagaaatg
     2581 aatgtaccac cttctaagaa agctaaattg ataataatcc caagtgaaga aaaaatagaa
     2641 gctattgagc ttggtaaaga ctattttata actttagcat ctgcttcaaa tgtagagata
     2701 gcaaaagata aatctaatgt tccagaagat gcagttggag tagttattga tggtgttgaa
     2761 atatttatac ctcttaatga attagttgat tttgaaaaag aaattgagag attatctaaa
     2821 gaaaagaaaa agttagaagg cgaaataaag agagttaatg gaaagcttgc aaatcaaggt
     2881 ttcttagcaa aagctcctga aagtttaatt gaagaagaaa aagcaaaaaa agaaaaattt
     2941 gaagagatga ttaaatctgt tgaggaaaga ttaacaaatt tagaatctaa aataaaataa
     3001 atataagaat tagtaaaaac atacaaaaag tatatattct atactttttg tatgtttttc
     3061 tttatatata tacaaatatg atgttataat tctttacaga tgtgttatat ttttatttta
     3121 tgttgtgaat atatgaattt tatgtcttaa aatttataag caa
//
LOCUS       DAELTA010000252         3116 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.257, whole genome shotgun sequence.
ACCESSION   DAELTA010000252 DAELTA010000000
VERSION     DAELTA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.257"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            131..1636
                     /locus_tag="KPG13_003502"
     CDS             131..1636
                     /locus_tag="KPG13_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG0987184.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2026..>3116
                     /gene="dinB"
                     /locus_tag="KPG13_003503"
     CDS             2026..>3116
                     /gene="dinB"
                     /locus_tag="KPG13_003503"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012581042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG0987185.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSI"
BASE COUNT         1246 a          331 c          559 g          980 t
ORIGIN      
        1 aattaatctt aattgtataa tattactttg agtgaaaaag tattaacatt tttttataag
       61 atgttttact aaaaaaatat atataaatat actgttatca acaagaaatt taatttagtg
      121 aggtgttaag atgaaagtag gatttgacca caaaaaatat ttagaagagc agtctaaata
      181 tatcttagaa agagtaaata attttgacaa actttattta gagtttggag gaaaattgat
      241 gggagacctt catgcaaaaa gagtacttcc aggatttgat gaaaatgcaa agataaaagt
      301 attacaacat atgaaagaaa aagttgaagt tgtaatttgt gtctatgcag gagatataga
      361 aagaaacaaa ataagaggag actttggtat cacttatgat atggaagttt taagattaat
      421 agatgattta agaggttatg aactagatgt aaatagtgta gtaattacta gatttgaagg
      481 acaacctgct acaactgtat ttataaataa attagaacgt agaggaatta aagtttataa
      541 gcattatcca acaaagggat atccgtcaga tgttgagact atagttagtg atgagggata
      601 tggagcaaat gaatatatag aaactacaaa gccaatagta gtagtcacag caccaggacc
      661 taatagtgga aaacttggta cttgtctaag tcagttgtat catgaaaata aaagaggtaa
      721 tgtagttgga tattctaagt ttgagacatt cccagtttgg aatgtacctg taaaacatcc
      781 tttgaatata gcttatgaag cagctacagt tgatttaaaa gatgtgaata tgatagattc
      841 attccacctt gaaacgtatg gtgaaatgtc agtaaactac aatagagatt tggaattatt
      901 tccagtgctt aaaaaaataa tagaaaagat aactggaaaa gaatcaattt tccaatctcc
      961 aactgatatg ggtgttaata gagttggctt tggaataata gatgatgaag taataagaaa
     1021 agcttctata gaagaaataa taagaagata ctttaaaact gcctgtgagt ataaaaaggg
     1081 acaagttgat aaaggtgctt atgatagaat gaaaatgatt atggaagagc ttaacttaaa
     1141 gccagaagat agaaatgttg tattacctgc tagaaactac agtaataaat tgaaagaaag
     1201 tgccgacaag aatgatactt gtcctgtagt tgcattagaa ttagaagatg gtacaatatt
     1261 aactggtaag ggttctgatt taatgaatgg aactgcagca gtagtattaa atgccattaa
     1321 acatttagcc aatatatctg atgatatgca cttaatatca cctgtaatat tagaacctat
     1381 cataaactta aaaacaaaga ctttagctag tagaaatgtt tcacttagtt gccaagaggt
     1441 gttaactgca cttagtattt gtgctgtaac taatccaact gcacaatttg ctatggaaaa
     1501 gttagcatta ttaaaagggt gtcaagctca ttctactact atattaaata gagatgatga
     1561 acaaacattt agaaaacttg gtatagatgt aacttgtgac ccagaatatc cttcacaaaa
     1621 tctttactat agttaaaatt atataaaaaa atatgggctg ttttaaagta atagtttgag
     1681 cagtccatta caattataat aaaatcagta ggctgtcttg tataagacag tcttttgttt
     1741 ttaaaacatc ccataaatta aatatatata ctgttatcca taaggaattt taaatttagt
     1801 tccttggaat acctattgta aagcttttat atagtttttg caagtgtttt taaggatttt
     1861 ttataaatta tattgaagtt aatggattat agatggtttt atttatggaa aatatgaata
     1921 tataaagaaa ataatgaaaa aataccaaaa agaaacataa gtttggtata attaaaaagt
     1981 aatataaata aaattaatct atttatactt taaaaggtga taataatgaa acattataga
     2041 aaaataattc atatagatat ggacgctttt tatgcatcta tagaacaaag agataataaa
     2101 aaattaaaag gaagacctgt tattgtcggt ggaaatccac aaagccgagg tgtggttgca
     2161 acctgttctt atgaagctag aaaatttggt atccattcag ctatgccatc agctgtagct
     2221 tacaataggt gtccatatgc agtttttgta agacctagat ttgatgttta taagagtgtg
     2281 tcagagcaaa taagagatat attttataga tacacagatt tagttgaacc attatcttta
     2341 gatgaagctt acttagatgt aacaaaaaat aaaaaaaata ttgattcatc aattgagata
     2401 gcaaagcaaa taaaaaaaga tatatttaga gaagttggtc ttacttcttc agctggtgtg
     2461 tcatacaata agtttcttgc taagattgca tcagacttaa gaaagccaaa tgggttaact
     2521 gtaataaccg aagaaaatgc ccaggacttt ttagataaat tacctgtgaa taaatttttt
     2581 ggtgttggaa aagttacttc aaatactctt aaaaatttag gtataaaaac tggatatgat
     2641 ttaaggtgtt taaatttatt tgaacttgaa aatatattca aaaaaagagg ttatgaatta
     2701 tataaatttg ctagaggaat agacgataga ccagtggaac caaatagggt tagaaaatct
     2761 gttggggctg agactacatt aagtcataat ttggatattg atgaagaaga aacacgcaac
     2821 attcttgatg aactttgtga agaagtatgt catagattaa aaaactctga gaagtttgga
     2881 aaaacattaa cattaaaaat taaatatgag gactttacaa agataactag aagccttagt
     2941 ttagaacact atattgacga atataatgat ataagaagtg gtgtagataa tttgttgaga
     3001 aatgttgagg ttaatggaaa acaaattagg ctattgggag ttacaatatc aaacctaagt
     3061 gataaaaaag aaacttataa agatattaca ttatttgaat acatggatag tatcca
//
LOCUS       DAELTA010000253         3080 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.258, whole genome shotgun sequence.
ACCESSION   DAELTA010000253 DAELTA010000000
VERSION     DAELTA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.258"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1192..1581
                     /locus_tag="KPG13_003504"
     CDS             1192..1581
                     /locus_tag="KPG13_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0987186.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2451..2921
                     /locus_tag="KPG13_003505"
     CDS             2451..2921
                     /locus_tag="KPG13_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG0987187.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT         1404 a          172 c          549 g          955 t
ORIGIN      
        1 gaaaatttat aatataatta atgaaaaaat ttaaactttg taatatataa ataaaaaaac
       61 tctaaaatag aaggatataa ttatatagaa tgtttcaatt aaacaaggta aaaactataa
      121 tcttaaaatt aaaattaaca ttattcttaa gaagcaagaa atttagaaag taggaagttt
      181 agtgataagt ggtagtaagt aagaaattta ggaagtggga agtttagtta taagtggtag
      241 taagtaagaa atttaggaag tgggaagttt agttataagt ggtagtaagt aagaaattta
      301 ggaagtggga agtttagtta taagtagtag taagtaagaa atttagtaag tgggaagttt
      361 agtagtaata atttatagca agtaagaaat ttaggaagta ggaagtttag taataatttg
      421 tagcaagtaa gaaacttagg gagtaggaag tttagtaata atttatagca agtaagaaat
      481 ttagggagta ggaagtttag taataatttg tagtaagtaa gaaatttaga aagtaggaaa
      541 tttagtaata atttatagta agtaagaaaa ttagggagta ggaagtttag taataattta
      601 tagtaagtaa gaaatttaga aagtaggaag tttagtaata atttatagca agtaagaaat
      661 ttagggagag gaagtttagt aataatttgt agcaagtaag aaatttagaa agtaggaagt
      721 ttagtagtaa tttatagtaa gtaagaaatt tagtaagtga gaagtttagt tataatttat
      781 agtaagtaag aaatttagta agtgagaagt tcagtaataa gttgtagtaa ataaaaagtt
      841 taggaagtag gaagttcagt aataagttgt agtaaataaa aaatttagga agtaggattt
      901 actgaaatac aaatatttaa aatcaaagag tttaaaagaa aggagagcta ctggacaagt
      961 atccaaaata gatagctgag tacaaaatat ttcataaaat taagcaatat aatacaagag
     1021 aattttgaca ttatttatat atatttaccg tgtacaaata gctaaattta ggattaagat
     1081 tgaaaaatat cttataagtc tgagcttata taaatccgta taagatgtat ttaatcgaaa
     1141 aattttatta tggatattgc ttgtctatat tctgagtagc aaggttttat catgactata
     1201 aaaaagttgc cacaatcaga attaaagata atgaaattta tatggaaatc agattccaaa
     1261 gtaacatcta gagatattgt tttaggaatg gagcaaaaat atcaatggaa acaaacgaca
     1321 actttgactc ttttatctag attagtagta aaaagatttc taaattctca aaagatagat
     1381 aaatatacgc attatgaagt tttaattaaa gaaaaggaat acataggagt tgagacaaga
     1441 gattttttta gaaatattca tgattcttct ataaaaagtt tattgttgtc attacatgaa
     1501 aatattaatt tgagcaaaga tgatatttta cttattgaag agtggataaa aaatctaaaa
     1561 gaagaggaga aagacgtata agcaaacgta tttcttatag catcaatata aagtgtatgt
     1621 aagtgtattt atgtaatata ttagtctact taagtaatat gacaaatgat tataagtgga
     1681 ttatgatact ataatgtgaa tctaaataaa ttaaatataa attaatagtg gatattctat
     1741 ttaattaaat ttaaaaagct acctttactt ataagtataa ttaaggtagc tttttaaata
     1801 gaggtagtgt aattaaagag aatttttaaa tataaattgt gaagctttaa tatagatgat
     1861 aatatatatt ttgcaaatat gacacagtgt tttcgtattt aagatgataa aataaatagg
     1921 aattaatgat gatattgaaa aaataaaaat aaaggattaa tataagatat taaagagata
     1981 gagataaaag tgttaacaga agatattgaa aaaataaaag attgatataa gatactgaag
     2041 aggtagaaac aaaagtatta atagaagata ttgaaaaaat aaaaacaaaa gattgataga
     2101 aagtactgaa tagatagaga taaaagtgtt aatagaagat attgaaaaaa taaaagattg
     2161 atataagata ctgaagaggt agaaacaaaa gtgttaatag aagatattga aaacataaaa
     2221 acaaaagatt gatagaaagt actgaataga tagagataaa agtgttaata gaagatattg
     2281 aaaaaataaa agattgatat aagatactga ataggtagaa acaaaagtgt taatagaaga
     2341 tgctaaaaaa gtaaaaataa aggattaata aaaggtactg aagagataga gaaaaaaatg
     2401 ttaatagaag atactgaaaa aatagaaata aaaaattagg aggaaaattt atgaaatatc
     2461 aggtttcatt attagcagtg aaggatattg aagtatcaaa aaaattttat aaagaattat
     2521 ttgagcaaga aattgaattg gacttaggca aaaatgtaac atttaaagga ggatttgcta
     2581 tacaagaaga cttcgcatgg cttacaggga ttaatccaga tacaattata caaaaatcaa
     2641 ataatatgga actttatttt gaggttgatg attttgacga atttattgaa aaactcaatt
     2701 attataaaga tgtagagttt gtccataagc cactaaaaca tgaatggcaa caaagagtag
     2761 tgcgtattta tgacccggat aagcatatta ttgagatagg agagtctatg gaagttatag
     2821 ctagaagata tttatctgag ggatactctg tagaagaaac ttctaaaata attcaacatc
     2881 caattaaatt tgtaaagcag tgtgaaaata gtaaggaatg attgtttaaa ttataattat
     2941 ttagtagaaa ttaattgtaa tagaactaat tcaagataat atataggact agtatttgac
     3001 aaatgtcata aattaaatta aaataaagaa aatattaata aagaaaaaat aaatataata
     3061 ttaaaatata ttaataaatt
//
LOCUS       DAELTA010000254         3075 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.259, whole genome shotgun sequence.
ACCESSION   DAELTA010000254 DAELTA010000000
VERSION     DAELTA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.259"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            164..958
                     /locus_tag="KPG13_003506"
     CDS             164..958
                     /locus_tag="KPG13_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987188.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            959..3019
                     /locus_tag="KPG13_003507"
     CDS             959..3019
                     /locus_tag="KPG13_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG0987189.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1182 a          317 c          505 g         1071 t
ORIGIN      
        1 ttacaaaaga tattataaac gaataaaaaa taataatttg tcagataaac ttgtaaattt
       61 ttatgaataa tccattctaa aacaaaaaac tgtgtataaa aaaggtttat atgttatatt
      121 tatataaata taacatatga acgagaaagg agtggtttga taaatgattg aggtagataa
      181 attgtgtaag tcttttacaa gggttgtaaa agatgataag aataaaagtt caataagaaa
      241 gctaaagaaa gtaaaaacta aaaaagaaga attttttgct gtaaataatg tttcatttaa
      301 agttatggaa ggtgaaattg ttggaatatt aggtccaaat ggtgcaggaa agactacatt
      361 acttagaatg ttaggtggca tattaacacc aacatcagga agtataaata tttctggata
      421 tgattattca atagatagaa attcagcaaa aaaagaaata ggttatctct ctggaaatac
      481 aaaactatat ggaaggcttt ctccgagaga acttctaaca acttttgcaa gtctatatga
      541 gatgtcaaaa gaagatatag aagaatcaat tgaaaatatt gtaaaaataa tggatatgag
      601 tgaatttata gataatagaa ttgaaaatct atcaactggt cagactcaaa gaacatctat
      661 agctcgttgt ttaatacatt ctccaaaagt atatattttt gatgagccaa cactaggtct
      721 ggatgtttta agtagtgcat ctattataga ttttatgaag aatgaaaaac taaggggaaa
      781 aactgttctt tactcaactc attatatgga agaagcagaa actttatgtg acaagatttt
      841 tatgattcac aatgggaaaa taattgcatc aggaacacca cagagtttaa aagaggaatc
      901 tggagtaaat aatttgagag atgtattcat aaaacttgcc agaaaggagg acatttaaat
      961 gagaagtaag attgtaaaat acatatttaa aaaagaaatg ttagacatat tgagagataa
     1021 aaagacttta tttatgatga ttgttgtgcc acttctttta tatcctttac ttatgttatt
     1081 aatgattcaa gtaatgaata tgtctgcaag tagtatgaat agtaaggaca ttaatatagc
     1141 ttttaataat aaacctaata aggtgttagt atccatgatt gaagatgata atttagaaaa
     1201 agatactaaa aatacttcaa atgataaagg caaaataaga gtgttagatg tagataacta
     1261 taaaaagtca ttagaagaaa ataaaattga tgcttatata aagatggaag aaaaaaaatc
     1321 atctataaat tatcaaatat atataaattc ttctcaagaa aattcttcta atgcactaaa
     1381 aagaattgaa actgttttgg ataaatataa aagatttacc atagaaaaaa ctttatctga
     1441 aaaaggatta gatgtacaag ctactttaga accaataaca tatagcactg tagatgttgc
     1501 aaagacggaa gaagtggcag gatacttttt aggtcagata cttccattta tattaataat
     1561 aggagtttta ctaggttcaa tatatccagc aatagatgtt atggctggag aaaaggaaag
     1621 aggtacatta gaaacattat ttacactgcc aatatcaaac ttagaactgg ttatgggaaa
     1681 atatatggca gtttctctta gtgcaatagt aacagcaatt ttaaacattt tatctatggg
     1741 actgactatg gcatttattt tagttagtgg aggtattagt agccaatttg gctttggaaa
     1801 ctttaattat ggagacttag tcttaccaat tataactact ttaatttgca tatgtctttt
     1861 ttcaatggta gtttcagcaa taagtatgtg tgtatgttca ttagcaaaaa gttttaaaga
     1921 tgctcaaaac tacataacac ctcttatgtt aattattatg ataccatcat atgtttctat
     1981 gataccaaat atagtattag atagagtgac agctgtaata cctgtagtaa atatatcttt
     2041 acttataaaa agtgtattat cttttaaaag cgatttaaat ttaatagcct tagttttagc
     2101 atcaaatgta gcctttgtta tattggcaat catactactg tctaagatgt tcaattctga
     2161 ggaaatactt tttggtaata gtaagagttt ttctttctta gagaaaagaa gtaatataaa
     2221 aaaagggtct ataccaactg taagtgatgg tgttatttta tatgcagtag gattagttct
     2281 attgatttat gttggttctt tagttcaaat taaatttggt atggcaggta ttataatgac
     2341 acaattcatg ataatttctt taccattatt atttgcatac tatattaaag ctgattttaa
     2401 agaggtattt agcttgacag ttcctaaatt aaagcatgtg tttgggagca ttgttctttg
     2461 gattggtgga tacatactta ttatgattat aacacagcta attttattct tatttcctca
     2521 aaatatggaa gttgctgaaa gtttaaataa ggctcttttt atcaaagata gtgttttact
     2581 gaatttacta gcaatagctt tattaccagc tatatgtgaa gaaatgtttt ttaggggatt
     2641 tatattttct tcatttagta aatcaaaaga taaaaataag tctatcaaac tcgctataat
     2701 atgtagtggg gtactatttg gtattatgca tatggatttt attagaataa ttccaacatc
     2761 aatattaggt attatatttg catacagtgt gtataaatct ggttcaatat ttgtatcaat
     2821 gttattgcat tttttaaata atagtgtagc agttgtgtta aatcattata caactggaaa
     2881 tataaccaac ttatataaat ttgttgaagt tgatttttcg aacttaaatg tgcttaaatt
     2941 tgccttaatt cttctaataa gtcttatatt aattatgtta ggattaagag ttttagatag
     3001 aaagtcttta agaaattaag tataaagtta ttatatagat taaaatatta ataagcacta
     3061 aagtatatgg atata
//
LOCUS       DAELTA010000255         3005 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.260, whole genome shotgun sequence.
ACCESSION   DAELTA010000255 DAELTA010000000
VERSION     DAELTA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.260"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(6..614)
                     /locus_tag="KPG13_003508"
     CDS             complement(6..614)
                     /locus_tag="KPG13_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG0987190.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(723..>3005)
                     /locus_tag="KPG13_003509"
     CDS             complement(723..>3005)
                     /locus_tag="KPG13_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG0987191.1"
                     /translation="ISMFNLLYLLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKI
                     NIVDSDRLIGVIYLLLCNHTYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINL
                     AIEKDMIIQRTLKNYIDQRKELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDM
                     NNYYKILTFIKEDEKVAEKLKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISL
                     NDITNEIKIDEEIRNINKDYENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKC
                     VSSDLVDMTLEQYFETFNIDFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDS
                     EGEEHVEEVRVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIF
                     LEDLSTSRVLYTNRAFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIV
                     NENNHIKEVKMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFY
                     ANISHELKTPLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNI
                     INISQVKSDIYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPE
                     SIERIILNILSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFK
                     QIENSGISNEFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYD
                     NSNYNDNIEKFEIEFFDIYK"
BASE COUNT          952 a          487 c          243 g         1323 t
ORIGIN      
        1 aaaacttatt ctatataaaa acgccataaa aaatcttttg cttcttctgc ataatctata
       61 tttatacgtt tactagtttt tatatctaac tcatctttac taaatacttt ttttccttta
      121 tcatcataat aaaaatcact tatatataat tcttcaccca ttatactctt actatttaga
      181 cttctatcta tttttaaagc tttgcaaagt tttcctggtc cgttagtaat attttttact
      241 tgatatttac tcaactcagt gtaagctttg ttgtatctat tcatagatat ctcatctaaa
      301 gaagttatag gctctacccc tcttattaat acacactctg gcacattttc tatatttgca
      361 gataaattca agcaattata cataccataa attaaataaa catatatatg tccaccatct
      421 aaatataatg gcttagttct atctgtcttt ttattgccat atacatgtgc tcctttatca
      481 tttacaccca tgtatgcttc tgtctctatg attttggtaa caattacttt attttcatat
      541 ttccttatta gatatttacc taatattgat ttggctaaat caattccatt ttttctaaaa
      601 aaatcctttt ccaaaatttc acctcataaa atttatttaa aacgcatcaa atacaactaa
      661 agtaataaat aaaattaact cttattaatg tgatatatat aaacaataaa tttagacatt
      721 tattatttat atatatcaaa aaattctatc tcaaactttt caatattatc attgtaattt
      781 gaattatcat atacttcttc aacaacctct ttaattggga atataacttt tacattagtt
      841 ccttctccta ccttactatc tatgtaaatc ttcccatttt gaatttcaat caatgatttt
      901 gtaaggcata aacctatacc acttccaaat tcatttgata taccactgtt ttcaatttgt
      961 ttaaatcgat taaatatatc ctttaatttt tctttgtcta tgcccactcc agtatctttt
     1021 ataattatct ctactgtttc gtctttttta tatataccta caaggatttc tcctccttct
     1081 aaagtaaatt ttatagcatt tgataatata tttagtatta ttctttctat cgattctggg
     1141 tcaagcccta ccataacagt ttcttcatct gtatcaaaaa ttaaatctat tcctttagac
     1201 ttagcataac tactaataga tgtaactatt ctttctgtta tgtcaataat atcaaaatta
     1261 actgctttaa tcttatatat gtctgactta acttgagaaa tatttattat attatctatt
     1321 aatctcaata atctaaacat attttgttta gttattttta tatgttcttt tagtatatcc
     1381 tgaaaatcaa ttatttttcc ttttttataa aggttttcaa taagttgaac tgaactatat
     1441 atattattta aaggtgtctt aagttcatga gatatattag cataaaactc catcttcatt
     1501 tggtcatact gtctttgttt tattaatgct tttttaagtc tttcagactc tctttgcaca
     1561 cttaaatctt taataattct cactctactt cttttctgtc caaagtataa tgttcttgat
     1621 atcattttta cttctttaat gtgattattt tcatttacta tatggatact cttctctctt
     1681 tctcctatat ttaaattgtt tatgtatttt tttactaata tatttctata tttttgtgtt
     1741 gtaactccta atacatcact agaaattccg aacaactctc taaaggctct atttgtatat
     1801 agtactcttg atgtacttaa atcctcaaga aaaatagcct ctggtataac atccagtaat
     1861 ttttcatata ttattttttg ttcatttact gttctaagaa tatttatctc tttagttata
     1921 tctctaaatg taagcaattt aaagagttta ttatctgacc aatagtctcc aactctaact
     1981 tcttcaacat gttcttcacc ttctgagtca gacatcacaa aattaactat acagtttgct
     2041 gtgtcattgt aacttactat ttcagctttc ttttcttgtg ttagatttaa tcttttaaat
     2101 tccctattgt caaagaaatc tatgttaaaa gtttcaaaat attgttctaa tgtcatatcg
     2161 actaaatcac ttgaaacaca tttaaaactc ttttcatatt ttttatttat acttattacc
     2221 ttattcttct ttatgccacc ttcagcactt cttacaagta taggagatgg aatatttagt
     2281 ataatgtttt catagtcttt atttatattt cttatttctt catctatttt gatttcattt
     2341 gtaatatcat ttaaacttat taagtaattg ctttcgtctt tattattact taaacttaac
     2401 aaataaattt tatcatttaa ctctaccagt ttatcaatta tacttatatc gttatcaaat
     2461 ttcttcaatt tttcagcaac tttttcatct tcctttataa aagttaatat tttataataa
     2521 ttattcatat cctttgaaaa ttcatcttta aatttattat ttgtgtaact tatattaaaa
     2581 tcatagtctg ttactatcca tacatttggt attgtatcca gagcttgtct aagtaattct
     2641 tttctctggt ctatgtagtt ttttaatgtt ctttgtataa tcatatcctt ttctattgct
     2701 aaatttatct ttcctaattg ctctgacttt ttacttaatt ttttattgat tgaatcaagt
     2761 tcataatatg gatacactac tctattatta agactgtata catatacata ataagtatgg
     2821 ttacataata gtaaatatat tactcctatt aatctatctg aatctactat attaatctta
     2881 gaaattgcta caaaacatat aatagttgta aaaaaaacaa aatatgttaa atcttttatt
     2941 aacttatagt gctcaatgtt actatcaagt ttattcaaca aatatagtaa attaaacata
     3001 gatat
//
LOCUS       DAELTA010000256         2982 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.261, whole genome shotgun sequence.
ACCESSION   DAELTA010000256 DAELTA010000000
VERSION     DAELTA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.261"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     repeat_region   382..674
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=382..410
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(961..1185)
                     /locus_tag="KPG13_003510"
     CDS             complement(961..1185)
                     /locus_tag="KPG13_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0987192.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            1896..2051
                     /locus_tag="KPG13_003511"
     CDS             1896..2051
                     /locus_tag="KPG13_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG0987193.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            2297..2548
                     /locus_tag="KPG13_003512"
     CDS             2297..2548
                     /locus_tag="KPG13_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987194.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
BASE COUNT         1246 a          280 c          436 g         1020 t
ORIGIN      
        1 ataataatct aaagtggcaa gcatgtcagc tggtgttata tcagtttata tttatgataa
       61 aataaaaatc tcccaaacgc caataagaat gatttaaaaa aataatattt tcacttaaaa
      121 ataatcactc tttataggag taaattattt tcttgatttt attatactac aaattggtac
      181 agatatttaa aaataatata ttcataatat tataaatagt tttgcagtga gcgatatttt
      241 tgataaaata gggcttaaca gttgaaacat aaggcattga gggtatatga taaatattat
      301 catttgcact actcgtggtt cactgcaaat ttgagagaat tgtatagatg taagtcttgg
      361 aaatgctcaa tttattttgg ggttttatat taactaagtg gtatgtaaag aagtgttgat
      421 atttatattg tctatccccg ctagcttgtt ttatattaac taagtggtat gtaaagtcgt
      481 aaattacgac cgtacctttc gtaaaataac aatcgtttta tattaactaa gtggtatgta
      541 aagctttttg agtatgagta caagcatagg aaaattaata gttttatatt aactaagtgg
      601 tatgtaaaga acaaaaaaga tacatctgta actaattttt atactgtttt atattaatta
      661 agtggtatgt aaagacagat ataatatcaa aataaggtta ggacttttaa aagcatagcc
      721 atatcagtaa aaatttaatt atcatttaaa aaataaattt ttatttaaag aatacacata
      781 aatatttcca gactcaactc tcttaaacta ttttcatatc ttttagtaga tacctttgtt
      841 gttttttaat ctttagcaac ttgttcttga gtgaacctct tattttttct aagttatttc
      901 aagcttttca aaaaattctc tatttatatt catcacacat ataatacaat taattcagct
      961 ttaattgcca atatttagtt tttctgtatc tgataaacca agaatataat cagtatataa
     1021 accaaaaatt ttagcaaata ttattaaccc atcgtctctt gttggtcttt caccagactc
     1081 tattctattc ataacacttg tatttatatt tgttttttca agcaattctt tttgagaact
     1141 attcatattt tccctaatat atttaatcct ttgatttaca tccatacata attatctcga
     1201 aaagtttctg ttttggaaat attgttattg aaatatagca attaatgtga atatatgaaa
     1261 aagatacact atgattttat aaaaaagaag acatacataa aatttatact tacaaagtgt
     1321 tgcaaatgag ttaggtttta aaaatgcatc aatttatttt aagtacagga aaggggaata
     1381 ttcaattaga gttgatatgt tacctataaa ctatctaaga tacttgatta tgatatagaa
     1441 aattttttta caaattaaat ttctaagata gaaatttaat taagaggatg atgaggatga
     1501 ataatttaca agtaatataa agaaataata agagaatttt aaatgcatag caactagcag
     1561 atatatatga aaatgatgta aacaatatat aaactaactt caatagaaat aaagagaatg
     1621 taaattatgt tttattacaa ttcgagtatt tgaaagaatt taagaactaa tctattaata
     1681 gtcagttgat ttcaaaacat tctagtcaat tatacctttg gatcgaaaga tgtgcaaata
     1741 gacactgcaa aatattagat attgataaaa cttgggaaca atttgacaat ttagagaaaa
     1801 tatatttcaa gattaagcga gagaaactaa gctgtataga agatgtatta atagaaaatt
     1861 taagatttaa ggatacaaat taataaagca ataatatggc attagatgta aaaatagaag
     1921 ttaaaataat aaaagatgtt gttatattaa atataacaca ttggaaaaaa gaaacaagtc
     1981 tattaacagt taaaatagct agaagtcaag gtgatttaag ttatgtaaaa tattttgcta
     2041 atgagagcta agagcttctt aacaagagtt ttggtgtgtc tcttgaaaca aggttaacaa
     2101 ttaataaaag acgaatggca gaagaaggcg tatgtaagtt taaaagagat aagttaaaca
     2161 aactagatac tatgtaagag gataagaaac ttatagaaga atatgttact tttgtaaagg
     2221 atttagcttt aaaatataga atataaagtg attttaagta aaaattgtaa caattaaata
     2281 agtataagac aagtttatga gttcatattt tataacaagg ggtgatgtta tgaaaatact
     2341 acaacaattt aaaccataca aatatgataa agaaaaaaat atagaatatg atgaaaaaat
     2401 tatagctaat caaaacacag aagtttatat agtatatcca cgtatatctg atgaagaaaa
     2461 taggagaaga tgggaagagt tcaatgaagt agctaaagaa gtagctatta atatagctat
     2521 gaagaaagtt gagacagtag aaagttaaga gaatagagaa gttgctaata cttagcactt
     2581 tctcatagaa gaaaattgga caactatttt taaataatta ggaataaaaa agttaaatca
     2641 tatttgaaaa aaggcgataa aattgttgag tattgttatt agtaataaga atatattaac
     2701 tatgtattaa aagaaattat aagtttttag taaatatagt atttaaggat attaaaactg
     2761 gtaaaaaata tcatttagac tattaaataa aaaagtgctg gtcaaagtag caacacaata
     2821 aataaaaaaa cttatagaaa ttataagata aaaagatgag atttgaaatg gtcgaagtta
     2881 atataaaaat taaaggtgaa gtaagtgaaa taattaactc aattagaggt atatttaata
     2941 gctttgatga agaattaata ttagatataa aatcagagta ag
//
LOCUS       DAELTA010000257         2904 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.262, whole genome shotgun sequence.
ACCESSION   DAELTA010000257 DAELTA010000000
VERSION     DAELTA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.262"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            148..2283
                     /gene="rnr"
                     /locus_tag="KPG13_003513"
     CDS             148..2283
                     /gene="rnr"
                     /locus_tag="KPG13_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG0987195.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1293 a          271 c          516 g          824 t
ORIGIN      
        1 ttaaatcaaa aatataaata agtaatttta acttataata ataagaaaat atagaaaatt
       61 atgtgataat taaaaaatgt tttgtctgaa gaaatataaa agacaatata aataataata
      121 taatgaaaag taaaacagga gggaaaaatg ataccaggac ttaaagaaag tttgctagga
      181 ttaataaatg aaagtgcata taatcctcta aaaaaagaag aattagcaga aatatttgat
      241 atacattcat cagaaatgcc aatgttttat aattttttaa atgaattaga agaagatggt
      301 tatatctgct ttactaaaaa aggaaaaata gtatctccca atcaaatggg atactttgtt
      361 ggaaaatttg tatcacacaa aaagggtttt ggatttgtag aatctgatgt tgaatataca
      421 caggatttat ttatctcaag tgacaatata aatggtgcta tgcataatga tagagttatg
      481 gcagaaatag tagtacctgc aactgaagaa aagagagcag aaggaagaat agtaaaagta
      541 ataaagagag aaataactag aatagtaggt actttccaat caagtaaaac ttttggattt
      601 gtaacaccag acaacaagaa atttactaaa gatatatata tacctaaaaa acattttagt
      661 ggagcagtag ataatgataa ggtagtatgt gaaattacag tatggccaca agaggataga
      721 aaaccagaag gcaaaattat agagatacta ggtcaaaaag gtgaaagagg cgtagaaata
      781 gattctataa taagagaaca tggtcttcca gaagaatttc caaagaaagt attgcaagaa
      841 gcagaagctg tagctgttga gattcctcaa gaagagataa aaagaagaag agatttaaga
      901 gatttaaaca tatttactat agatggagat gacgcaaaag acttagatga tgccatatca
      961 atagaagtat tacctaatgg aaacttcaaa ctaggagttc acatagctga tgttacacat
     1021 tatgtaagag agaaaaataa acttgacaag gaagccttaa aaagagctac atcagtttat
     1081 ttagtagata aagtaattcc aatgttgcca aaaactcttt caaatggagt atgtagctta
     1141 aatccatttg aagataaatt aactctttct atatttatgg aaatagacca taaaggaaat
     1201 gtggttaaac atgaaatatg tgaatctgta ataaattcaa aagctagaat gacttataca
     1261 gaagtatctg atatattaga aaaggatgat gaaaaactta aaaagacttt tgaacatgtt
     1321 gttgaagatt ttaaaaatgc tgaaatttta gctaggattc ttatgtcaag aagggaaaaa
     1381 agaggagcta tagacttcaa tttcccagag gcaaagataa ttctaaatgg aaaaggcgaa
     1441 gttgtagata taaagccata tgaaagaaga atatctaata agataataga agaatttatg
     1501 cttataagta atgagacaat tgccgaacat ttttactgga tgggaatacc gtttgtgtat
     1561 agaatacatg agacaccatc tttggaaaaa atggaagaat tgagtaagtt tatatctaca
     1621 tttggatata taataaaagg tgataaagag gaagtacatc caaaagcatt acaaggtata
     1681 atagagaaaa taaagggcaa aaaagaagaa ggagctatca gtactataat gcttcgttct
     1741 ttaaaacaag ctaagtactc accagaatgt agtggacatt ttggattagc agctaaattc
     1801 tactgtcact ttacttcacc aataagaaga tatcctgacc ttcaaattca tagaataata
     1861 aaagaatctt taaataataa aataagtgga aaaagacaag aacagcttac tactatagtg
     1921 gattatgcat ctgttcaatc atccgagaaa gaaagaaagg cagagttagc tgaaagagat
     1981 gtacatgatt tctataaggc tctttatatg aaggataaag ttggtcaaga atttgagggt
     2041 gttgtatcta gcgtaacttc atttggaata tttgttgagt tggataatac catagaagga
     2101 ttgataagac ttgcaaatat gaatgatgat tactatattt atgatgagag ttcttatagt
     2161 gttttaggag aaagaactaa gaagtcatat aagataggag accttgttaa gataaaggtt
     2221 gaaagtgtta atgtagattt taaagaaatt gactttgaga tagttgaaaa aatagaggag
     2281 tagataaaac agctaaaata agagataaca agttgtagta tttatgatat gtataaatat
     2341 atagtgcaca atactctata aaataaatga taaaaatagg taattgagaa atatctaatg
     2401 gaaatagtta actgaaagat aaataattaa gaaatattta atgaaagtag ctaattgaaa
     2461 aataagtaat tgagaaatgt ttaatagaaa gagctaactg aaagataagt aattaagaaa
     2521 catttaatag aaaaagccaa ctgaaaaata aataattaag aaatgtttaa tagaaagagc
     2581 taactgaaag ataagtaatt aagaaacatt taatagaaaa agccaactga aaaataaata
     2641 attaagaaat gtttaatata agtggctaat tgaaaaataa gtaattaaga aatgcttaat
     2701 ataagtggct aattgaaaaa taagtaattg agaaacattt aatagaaaaa gctagctgaa
     2761 agataagtaa ttaagaaata tttaatgtaa acagataatt taaaaatacc taattaagaa
     2821 tagatattaa aaaatataat ttcaatataa aaataagtgt atcatttaaa atttaagata
     2881 cacttatttt ttatttaaga taac
//
LOCUS       DAELTA010000258         2802 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.263, whole genome shotgun sequence.
ACCESSION   DAELTA010000258 DAELTA010000000
VERSION     DAELTA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.263"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            349..1716
                     /locus_tag="KPG13_003514"
     CDS             349..1716
                     /locus_tag="KPG13_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG0987196.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1741..2349
                     /locus_tag="KPG13_003515"
     CDS             1741..2349
                     /locus_tag="KPG13_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG0987197.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2397..2768
                     /locus_tag="KPG13_003516"
     CDS             2397..2768
                     /locus_tag="KPG13_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG0987198.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
BASE COUNT         1201 a          206 c          462 g          933 t
ORIGIN      
        1 atttttattt aattaagcat tgatttatca ttttttatat ttcatataac aatgcaaaaa
       61 ataattttta tatagtaaat gacaaaagtt tacaataaat gtatattttc ttactcattc
      121 cttaaaatta ctcatttagt ttaataactc tcatttcata tgtaaagttg gtaaaaaaat
      181 taaaattaaa agtaaaaaac taaataaatt aatgttaatt tgaaatatat atgaaggttt
      241 ttggagaata cttacttatg ataggaaaaa atgttaaaat aatagtgtaa tcaaatataa
      301 aggactgaaa taattaacag ttattaaata ttaggagtgt aaaaatttat gggattaaaa
      361 gaatttgatt ttattgaaga gtctattgat atgttgagga caatgtcacc aacattagaa
      421 actatatcag atgaaataga agaatatttt gaaaatatct tggatgaaaa gaatcaagaa
      481 tatataaatg taacctctag aataaaatca gaatcaagtt taagagaaaa gataattaga
      541 aatagatatt taaaaaaata tggagaagca agtaatctta ttcataatgt ttctgactta
      601 ataggcctaa gaattgagtg tagatttatt gaggatgaaa acaaaatata tagacttttg
      661 agaagatatt ttaataaaac agatgacaaa ataaactatt ataataaaga gaataagaat
      721 attaaattaa aactatctga gaggcaaccc aataagcaaa aaaatggatt tgaaatatat
      781 aaaatagatg gagtttttgc gtatttagat agagaagtaa aatttgaact tcaaattaaa
      841 tctcttgtaa atgtgttttg gagtgaaata gaacataaaa taatttataa aaacaataca
      901 tatttacttg ctgataaatt tataaaggat atgatggact ctataaagaa taatcttact
      961 atgatagata atcagctttt aagtatttat aaaaactttc attcagggaa gtcttttaat
     1021 atgaaagtaa gtaaaaaaga aattgaaaaa ttatttgcga aactagtcta tgacgctttt
     1081 tctgaaaaga tgaataaaag tataggtttt gttgtagatt ttaaaaaacc atgtgagact
     1141 atattgaact attcttttaa taaacaagat ataagtgatg aagttttagg taattttatg
     1201 cttgatgagt ttgcaagatt aaatgaaatt gtaaacaagg atatagattt taatgagcaa
     1261 atagaatttg aaagagaacc aacatttgat gataagtttt gtaaagacct aggaaatcat
     1321 tttagaagta gattaaacac agaatttcca tggaacctat tttttagaat attatttgaa
     1381 atagagccat ataacaatac caaagacttt gaaaactttg tagagtttat aaaagaaaat
     1441 gtgctactag aagaaaatag ggagcaatta ttatgccaat ttgaagagga tagtaagctt
     1501 ataattgaag atatatatga ttgtatattt aatagtataa gtgagattga tagtgttgaa
     1561 attttatata actataatct tgaaaaaata aactttacat caagtgaaat cataaattgt
     1621 atatgtagag aatatgagtg ttatggtgaa tacttagaag aaaaagaaaa gcttatgaat
     1681 acatttaaag aaaagattat tcaaatcttt gagtaatagg tcaatgctga agtggcgcta
     1741 atgcgccatt tttcaaatat atatgaatta agtaaaatta ttttggagga ggcgatttgt
     1801 atagagaaaa caggtgtaat tttggcaggg ggaaaaaatt ctagaatggg tagagataaa
     1861 gcctttttag agttacatga taagcttttt attgaaattg caatagaagc atttaaaaac
     1921 tttgatgagt tgataataat atctaacaat gaagaattat attctaaata cgatattaaa
     1981 gtttataatg atatagtaaa agatgttggt ccaattggag gaatatatac tgctcttgaa
     2041 tatgcaaagt atgatatagt tacaattgca tgtgatatgc catatctaaa taatcaaata
     2101 gtcgaaagaa tagctaataa aatggatgat aaaagtgtga tttcagtgac aaatggaaag
     2161 ttacaaccat tgtgttctgg atataaaaag agtataataa ataaagtatc tttatgtata
     2221 agggaaaatg atttaaagct cagaagcttt atagataaaa tagataaaag ttatatatac
     2281 tttgatgaag aagatttatt tttaaatgta aacactgttg atgagtataa aaaattaact
     2341 aaagtttaat tagctatggt tgtaaaatag agtaggattt atatggagga gagtttatga
     2401 atttacttaa atgcgattct tgggcagtaa tattaaataa tagcgataaa gaaagtaaag
     2461 catataaaat attagatgaa cttaaaagga atatgtacaa agttgttgct atagatgaag
     2521 agaaaaaacc tattgaagga atagatgtgt atgaatgttt aaaagatgtt ccacacaata
     2581 ttgatgttgt agctattatt gataaacaat ctaagatgga tgtgatttta gaagaagtag
     2641 agcttttgga tatacaaaat atgtggtttg aaaaaggaag ctttagtgaa atgatgatta
     2701 aaaagactaa agatttaaaa ttaaatatag aatataattt aagtttatat gatgagttga
     2761 ctagataaaa ttatatttct taataataaa aatataatta gt
//
LOCUS       DAELTA010000259         2732 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.264, whole genome shotgun sequence.
ACCESSION   DAELTA010000259 DAELTA010000000
VERSION     DAELTA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.264"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            327..1043
                     /locus_tag="KPG13_003517"
     CDS             327..1043
                     /locus_tag="KPG13_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0987199.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            1012..2604
                     /locus_tag="KPG13_003518"
     CDS             1012..2604
                     /locus_tag="KPG13_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0987200.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         1088 a          286 c          375 g          983 t
ORIGIN      
        1 aactacatta aaataaatat ttgttataaa ttcttaataa ttatgaaact catttgttat
       61 ttatatttgt taatatatgt ttaaacctaa aaaattaata aaccaaaata acataaggag
      121 tgtgatagat taccctagat attttcataa acttttacct tcagattaat atgggcgtct
      181 atacttttaa atttactata tgcattgaca atatattaaa agtactagtt tttatttcat
      241 aatgaacggg cttactgttt tctaatacct ctttttggta taaaataaaa attggttaaa
      301 aataaaattt caagggggtt aataagttga atcaaaatat attggttgta gatgatgata
      361 gagagatagt tgatgctata gagttctatt tgagacctga taactttaat gttttaaagg
      421 catatgatgg acttgaagca ttggacatac taatcgaaaa tgatattaag ttaataataa
      481 tggatgtaat gatgcccaat ttagatgggc ttaaaactac acttaaaata agagaaaaaa
      541 ggaatatacc aataattctc ttatctgcaa aatcagaaga tatggacaaa attataggtc
      601 ttaatatggg tgctgatgac tatataacta aaccatttaa tccactagaa cttactgctc
      661 gagtaaaatc tcaacttaga aggtatataa atttaggtga ttttagtaat acaaataact
      721 caaatgatac taattatatt ctaaaaagtg gtggcctaga gctaaataca gatacaaaaa
      781 tagtttcttt agatggtgaa gaaattaaaa tgacaccact tgaatataaa atacttacac
      841 ttcttatgtc acgaaaaggt aaaattttct cttctcatga gatttatgaa aatgtctgga
      901 atgaggatgc ttatagttgt gagagaacta ttgctgttca tattagaaga attagagaaa
      961 agattgaaat taatccaaaa gaaccaaaat atttaaaggt ggtgtggggc attggttaca
     1021 aaattgaaaa acttaattat taatgattct aaagcaaaat ttattttatt attattactg
     1081 tttctattag tttctatatt atttgaagca gtttatgatt tgaataataa cttttcttat
     1141 aatattaatt attattataa agagtttgct ctcccttatt tttatttttt cggtactcct
     1201 gtacatttgt caaaagtaga attagagttt gtctgcatag gtattatatt atcaagtttt
     1261 ttattgacta tactatgttt tacattgtat aaaaaaaata aacagactaa agtcaaattc
     1321 ttagagaatg tcataagcct tttttctaag gtaaaaatag aacttacact tatattaact
     1381 ttgatactat taaccttaag gtttcatgca tttacaagta ataaaatgtt ttacaacact
     1441 aatttaataa tctacaattt tataattata tctatagtct atataatata tgaaaatatt
     1501 aaatataata actataatct aaaagatatg tctataacat atgtattttt ttcaaacttg
     1561 cttaatatgt ataataaaaa aactataaat aaaaaattag ctatagtatt tttaatatct
     1621 atagttgtac aagctattat ccaatatctt gttttgatat tctttatgta tatatattat
     1681 gctgaggcaa tagctatagt aattttattt acacttgtaa acacagtttt tcatttttat
     1741 atatacaact ttttatctgt taagtttaat tacataaata acatatcaaa aaatataaaa
     1801 aatatagaaa atggcaattt aaagtataaa ttagaagtta taggagatga tgaaatatct
     1861 actttagcca aaagtataaa taatataaca ggtggtcttg aaactgctgt agatgctaat
     1921 ataaaaaatg aaaggatgaa ggcagagctt atcacaaacg tatctcatga cttaaaaact
     1981 ccacttacat ctatattaag ctatgttgat atgttaaaaa ataatgactt tgacagagag
     2041 actataaatg actatataaa tattttagat aaaaaatcac aaagattaaa attattaata
     2101 gatgacatat ttgaagcatc taagctaagt agtggagatg tagaatttaa tataacaaaa
     2161 accgatataa gagaactttt aattcaaagt attgtagagt ttgaagacaa aattcaaaat
     2221 agcagtttag attttataat agaaacacct gaatatgctg tttttaccat gatagatggt
     2281 aaaaaaactt ggagagtttt tgagaatcta atttgcaata tactaaaata ctctatgcct
     2341 aacactaggg tctacataga tatgtttata aaggaagaaa atataatatt aacgtttaaa
     2401 aatatttcaa atgataaact aaatctaaag ccagaagagt taatagaaag atttagaagg
     2461 ggtgatatat ctagaaaaac agatggctct ggtcttggtt tatcaattgc tcaaaatata
     2521 attgaattag aaaatgcaca catggaaata attatagatg ctgatttatt taaggtaatg
     2581 cttactttta gaagagttaa ttgatatatt gatataaaaa tatattttaa gttatttaaa
     2641 caagaaaaaa acctccttag aggtcttctt ctaaatgttg gggtttatat ttacatattt
     2701 tttgattata cctttttggt gtgatattaa ta
//
LOCUS       DAELTA010000260         2720 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.265, whole genome shotgun sequence.
ACCESSION   DAELTA010000260 DAELTA010000000
VERSION     DAELTA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.265"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            152..1075
                     /locus_tag="KPG13_003519"
     CDS             152..1075
                     /locus_tag="KPG13_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG0987201.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1050..1877
                     /gene="panB"
                     /locus_tag="KPG13_003520"
     CDS             1050..1877
                     /gene="panB"
                     /locus_tag="KPG13_003520"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG0987202.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1896..>2720
                     /locus_tag="KPG13_003521"
     CDS             1896..>2720
                     /locus_tag="KPG13_003521"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG0987203.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDN"
BASE COUNT         1082 a          332 c          452 g          854 t
ORIGIN      
        1 gtaagatatt tatggatggt atcttgttat aagaactata ctttaaatgt ttttaattat
       61 tttgtcttat gtaactcata tcctcaggaa tgagattaga aattaagatg ttgtatcaca
      121 aagtggaggt gatactatta aaataggctt tattggttct ggaaaagttg gaacatcttt
      181 ggcaaattat tttttatcaa aaggttattg tgtttcaggc ttttatggaa ggaatcaatt
      241 atctttatta gaatcaacaa atttaacaaa aacaaagatt tacagcaatt tgaaagatat
      301 aatttatgaa aacgatatat tatttattac aacatcagat gattctattg aaattataga
      361 caaaaaactt tccaaattta atttaaaaca taaatacatt tgccacacaa gtggctcttt
      421 aaaatctagt atattgttct gttcaaaaaa agcaggtgca ttaatttatt ctatccatcc
      481 tatatttgca ttttccaata aaaatactga cataaaaaaa atgaaagaca tttgtttttc
      541 catagaaggt gataatgaac aagatgattt agttattcaa aatttcatag atgatttggg
      601 aaaccaattt tttataagag ataaggatac ttcatccact tatcatttag ctaatgtttt
      661 agtttcaaat ttagtattat ctttactaga tataggagta agttacttta ttaaattagg
      721 tttatctgaa gaggaatctt taaaagctat cagtccttta gtaaaaaaaa acatagaaaa
      781 tatacttgat aatggcttta ctaaagctct tactggtcct gtttctcgtg gagatataac
      841 accaataagg aaacatctat ctgtattgga taaaaaagat gaggatattt acaaaatact
      901 atccttaaac ctactaaaaa taattgcctt aggaaatgac aattcattaa aaggtataaa
      961 aaatattact atagaaaatg caatcgaaaa tttaataagt aaatctgaaa aatacaaaga
     1021 aatttacaaa cttttaggag gaaaaaacga tgaaaaatac catacagact tttaagaatg
     1081 ctaaatatga aggaaaaaaa ctatctatgc ttactgctta tgactactca attgcaaaaa
     1141 taatggatga gtgtgatata aatggtattt taataggaga ttcattagga atggtcataa
     1201 aaggtgaaga aaacactcta tctgttacca tagacgaaat tatatatcat acaaaagctg
     1261 taaaaaatgg cgtaaaaaat gccttaatcg taagtgatat gccttttcta tcttaccatg
     1321 tttctataga agatgcagtt aaaaacgctg gtagacttat aaaagaagga ggagctcatg
     1381 ctgtaaagtt agaaggtggc tcaaacgtta taaagcaaat agaaagcatt gtaaatgctc
     1441 aaatacctgt aatggggcat ttaggattaa ctcctcaatc agtaaattca tttggtgggt
     1501 ttaaggttca aggaaatacc agtgaaacag caagacaact tattgaagat gctaaattaa
     1561 tagaaaaagc aggtgcattc tctattgtcc ttgaaggagt gcctacgaaa atagctgaga
     1621 tggtaactaa ttctatttca attcctacaa taggtatagg ggcaggaatt aactgtgatg
     1681 gacaaatttt agtttatcaa gatatgttgg gaatgtttgg agattttgtt cctaagtttg
     1741 taaagcagta tgcaaatata ggagatataa taaaagattc aattaaaaat tacatcctag
     1801 aagttaatac aggtgcattt ccacaagaaa aacacagctt ttcaataaat gaatctgagt
     1861 tagaaaaatt atactaatat taaggatggt acaaaatgct tgtaaaagaa attaaattac
     1921 taagaaatat aatcaaagat tggagaaaac atggctattc tataggcctt gttacaacaa
     1981 tgggattttt acatgaagga caccaaagtc ttataaaaaa agctgtaaaa gaaaatgata
     2041 aagtagtagt gagtatattt gtaaatccaa ctcaatttgg accaaatgaa gattttaata
     2101 gctatccaag agatatagat aaagatttta aatattgtat ggatagtgga gcaacagtag
     2161 tctttaaccc atcaccagaa gaaatgtatt taaaaggtaa ttgtactacc attaacgttt
     2221 ctggacttac agattttctt tgtggagcta aaagacctgt tcattttgga ggtgtctgcc
     2281 tagttgtttc aaagtttcta aatatagtaa cacctgataa agcttatttt ggagagaagg
     2341 atgcccaaca attagctgta attaaaagaa tggtcaagga tttaaacata gatacagaaa
     2401 taataggctg tcctataatt cgtgaaaatg atggattagc aaaaagttcg agaaatacat
     2461 atctctctga ggaagaacga aaatctgctc taatattaaa taagagctta agtttagcaa
     2521 aagaagaatt agtaaaaggc aatctaaatc cagagaatat aaaagaatta ataacagcta
     2581 aaataaattc tgaacactta gcaaaaatag actatgttga aattgtagat agtgagacat
     2641 tacaacctgt taagcaaata gaacattcta tacttgtggc aattgctgtg tttataggaa
     2701 aaaccagact aattgataat
//
LOCUS       DAELTA010000261         2689 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.266, whole genome shotgun sequence.
ACCESSION   DAELTA010000261 DAELTA010000000
VERSION     DAELTA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.266"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            685..1596
                     /locus_tag="KPG13_003522"
     CDS             685..1596
                     /locus_tag="KPG13_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0987204.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1771..2268
                     /locus_tag="KPG13_003523"
     CDS             1771..2268
                     /locus_tag="KPG13_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG0987205.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
BASE COUNT         1191 a          175 c          453 g          870 t
ORIGIN      
        1 aaatattgta ggaataatag ctaaaaagct ttaaaattag taatataaaa ttgaaaaata
       61 ataatataga gttgagaaat agtaaattga aattttaaaa taaatgagtg gaagtagctt
      121 agtaaagtat ggaaatgaat attacaatag aaaataaata atatgtaaag actttaaatt
      181 aattttaagt attaagtaca aatactaatt ttgatataga aattgaataa agtaaatgat
      241 aataagcaaa tgaattaaga tgataataag tgaattgata tagaaataag taaattaagt
      301 aagacgataa caagtgagat aataataaat gaattgacat agcaataagt aaattaagta
      361 agacgataac aaatgagatg ataataagta aattgatata gaaataagta aattaagtaa
      421 gatgataaca agtgaggtaa taataagtga attgatatag aaataagtaa attaagtaag
      481 acgataacaa atgagatgat aataagtaaa ttgatatagt aataaataaa ttaagatgat
      541 aatatccaaa gtgagaaaga taatagaaag taaataaaat catgtaaaaa taatttagat
      601 aagattctaa gattttattt atttaaaaat tagtatgatt aatttagatt aaataaattt
      661 aataatttta gtaggtggta atgaatgaaa ataaatagat taacagaaat aatagtaatt
      721 ctattaaata aaaaattagt aacagctaaa gagttggcag atagatttga agtttctaca
      781 agaaccatat atagagatat agaaactctg tctatgtcgg gagtaccagt atatatgacg
      841 aaaggcaaag gaggagggat atctttaata gaagaatatt ctatagacaa ggctatattg
      901 tcaaaaaaag ataaagagag tttaatagtt gcactgaaaa cattacaggc gactaagtat
      961 ccagaaataa attcagtagt aaataaaatt ggctcaatat ttggagagca gaatttttca
     1021 aactggattg aaatagactt tactgaatgg ggaagtaatt ttaatgaaga tgataaattt
     1081 acaaaaataa aagaagctat tttaaggcgt aatactataa attttaatta tgtaaatagt
     1141 ttgtcaagcc agacaaacag aactgtcgaa ccactgaagc ttatgtataa gagtaagact
     1201 tggtacttgt atggattttg taaactaaaa gatgatttta ggatatttag aattagtagg
     1261 ataaggaatt tatctataaa ggatgaagta ttttcaagaa agataataga agaagtatgt
     1321 ttgaatgatt ctaaagtaat taaggaaaat actataacgt tgaaattgag gtttaaagaa
     1381 aagatgcttt ttagagtatt tgatgatttt aataaggatt taataactaa aaatgaagat
     1441 gatacatatg atgtaattac agaatttcct attggtgaat ggatttatgg atatatttta
     1501 tcatttggtg acaatgttga agtacttgaa cctaaagatg ttagggataa tgtaataact
     1561 agactaaggg agttgtctaa aatttattca ttataatata atgctagatt aaagatttta
     1621 ttaaatatat aatatcttgc atttagtata taatatctca ctgagtttaa ttatattaaa
     1681 aatatgacat aaaggtgtcg tatattataa attataattg aattagttca aaataaaata
     1741 gattaataaa aacaaagtga ggtataaagt atgaattatg aaatagtaga gataaatgag
     1801 aaaatagtta taggaatatc gaaggagact acaaataaag atggacaagc ggtaaatgat
     1861 ataggtgaat tgtggaaaaa atttatgggc aaagggatat ataatgctat aaagggtaaa
     1921 aagaatgaca agacaatagg tctatataca gattaccagg gtgattttac aagtccgtat
     1981 agctttgttg catgttgtga agtaaatagt aattctgata aagagaagaa tttagaagaa
     2041 ttaaacacta ataatactgt tggagaaagt attattagta aagttatacc agcaggtaag
     2101 tatgcaaagt ttgttatagt aggaggtcaa aaagaagtag gagatttttg gtttgagttt
     2161 tggcaaatgg attttgatag gacttatata agtgattttg aagagtatca atgtaatact
     2221 tttgatacaa agaaacaaga aattcatatt tacataggaa taaagtagat ttgttattac
     2281 tatattgaat gaaatattga gtatagtaca tttattatag atagttttat tatttataac
     2341 tataagcagt aaatctgtaa aagatttact gcttttttag taattaatag tatcagtatc
     2401 actttgaatc ttcagacata ttcttcattt aatattgttt attgataaat gatacttgta
     2461 aatataatga tttgttattt aatttaaaat atgaaaaaaa gtattataaa cacattagaa
     2521 tacttctgga tttatgagta tacaatttga aaaatataaa aaataatgta taaagctaaa
     2581 tctaatttaa tcgataatac atatagagaa atataattaa gtaaataaac aactatctat
     2641 tagatattga gaaagtcaaa tcctatatga atctacagaa atgatttta
//
LOCUS       DAELTA010000262         2627 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.267, whole genome shotgun sequence.
ACCESSION   DAELTA010000262 DAELTA010000000
VERSION     DAELTA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.267"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            238..999
                     /locus_tag="KPG13_003524"
     CDS             238..999
                     /locus_tag="KPG13_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987206.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            1002..1820
                     /locus_tag="KPG13_003525"
     CDS             1002..1820
                     /locus_tag="KPG13_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG0987207.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            2000..2602
                     /locus_tag="KPG13_003526"
     CDS             2000..2602
                     /locus_tag="KPG13_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG0987208.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         1133 a          221 c          449 g          824 t
ORIGIN      
        1 taaaaagata tatttattaa atttgtgcta actgtacgga atatactgct taagtttaga
       61 ttagtgataa aaagatatat ttattaaatt tgaaccaact gtacaaagct atactaatta
      121 agcttagaat aactatataa aattataaca ataagtgtgt gggcagaaaa attctgctac
      181 acactttatt tatattcaca taaaaaattt atcaaaaaaa agaaaaagga gtaaaaaatg
      241 gaatttacaa gagaaccatt tatactagat gacgatttgt gtctagcttt ggtggacaaa
      301 agaattagtt atctaatgga aaatgaatta aaattaagaa atatagagat aataaaaaca
      361 atagaatgta aagaattata tgaaagcata aaatatcacc cggatatatg tatttgtaat
      421 ttaggaaaag gagatataat tgttgcgccg aatgtatata atcaatacaa agagttatta
      481 agtagatata aatttaatat aattaaggga gaagctaatt tattaaatag atacccttat
      541 gatatacctt acaatattgc gatagttggt gactatgcta tacacaattt taagtatact
      601 gacaaagcaa tattagatta tattgaaaat aataaactta aaaagataaa tatagagcag
      661 ggctattcta aatgctcaat ctgtatagta gatagaacgt ctataataac atcagataaa
      721 ggtatttgga aaagtatgaa gaatacagaa atagagtgtt tattgataga aaaaggtcac
      781 ataaatttat ttgagatgaa ttatggattt ataggtggat gtacagggtt gatttctaag
      841 gataaactag ctttttgtgg agatgttaaa aaacatcctg actatgaacg tataaaaagt
      901 tttgtagaaa gtaaaaacaa ggagatagtt acacttagct gtgaaaattt actagatata
      961 ggttctatag ttcctctcat gacaagaaag gagcgataac tttggttgag aaggataata
     1021 tttcttccat gaaatttttg aggaataata atgtaaacga tagattaata tatacgaatg
     1081 acattgtaga agtagtgata gattctgcaa agcaagaagt gttaaataaa aaaatactta
     1141 atggtactaa agctgaacaa agagctttaa atattgatga gatatcaacg gaaataacta
     1201 gtatgataat aagtataatg aaagagaaac ttttgaaaga atacattcta aaatgctatg
     1261 ggcgtacata tcctgaagat aaaaataata tatacatata ttcactgaac atatttgcta
     1321 aaaaagagat ttttatgaga gaaacagtat ttactagggt gagaaattat atagaagaaa
     1381 atgattttat aaatgtagaa ggttttatta gatttagaat gagagaattt atgaaatata
     1441 tttctgctat aggtgatatt gcagttgagg agtatttaat aaagaaagac caagatgaat
     1501 ttataagagt tttaaaatac tttatagata ctcaagaaga aaagattgat ttgcttaaag
     1561 ttcatataat ggaagataat acatttgttt tatacgataa aaatggaaat aagatagaca
     1621 gtatagatga tgaagaaatt ataaatatgg taataagaga aaatttaaat tatgaggact
     1681 ttttaataag taccttatta actctatgtc ccaaaaaaat agaaatattg gatttgttaa
     1741 ataataattg ctcaaaggaa attatagata ctgtagaagc aatttttgaa aataaagtta
     1801 gtataattat ggaaaactag aatctaagca tataaaagct atccatataa aattaaaaaa
     1861 attcttttta aatcgacaca aaagacttat aattgtatat aaagctatag tataactaga
     1921 gttattaagt ataaagaata tctacttgat taatgcaata tagctggcaa atatacaatt
     1981 gataaggagt aattttataa tggataattt aattaggata ctgatattag ctgttattgg
     2041 tggatttata ggttatgtta cgaatgtagt tgctataaga ctgatattta gaccaataga
     2101 gcctattaaa atacctattt taaatataga aatagtaggt cttataccaa agagaagagc
     2161 agaaatagca gcaaatgtag gagaaataat tcaagaagaa tttctttcta tggacgaaat
     2221 acttgcaaat atcattactg acgaagataa ggaagaagtt gtaagataca taaaagcaag
     2281 ggtaaaaatt ataatacatg agaaagtttc atttatcccg agtggtataa aaaacatgat
     2341 acaagattat ctaggagaga taattgagtc tgaagttaag caaagtatag atgaattgag
     2401 taaaaatata ataaataaag caaatgaacg tatagatata caaaaaatgg tcgaagataa
     2461 gattaatgaa ctagatttat atgaattgga agagataata ataagaatag ctaagaaaga
     2521 attaaaacat atagagtttt tggggcttgt cttagggttt ttaataggta tagctcaagg
     2581 gttaattact atgtttattt aaatatatga gaagtagcat ttattta
//
LOCUS       DAELTA010000263         2600 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.268, whole genome shotgun sequence.
ACCESSION   DAELTA010000263 DAELTA010000000
VERSION     DAELTA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.268"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(115..2160)
                     /gene="bclA1"
                     /locus_tag="KPG13_003527"
     CDS             complement(115..2160)
                     /gene="bclA1"
                     /locus_tag="KPG13_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG0987209.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          622 a          568 c          387 g         1023 t
ORIGIN      
        1 ttttattatt attgttattt tattcattct aaaataggta aaactcaata tataaaaata
       61 cttttgtcat ttaaataaag cctaaatagt ttttaagatt attttagaca cgttttaagt
      121 cataacagta tcagctattc taaatatcgt caaagtagcc gcagttcctg ctgattgccc
      181 atttcgtatc cctactgttg tagatgaaaa attatttatt gtaaaagtag tcccaccaaa
      241 taaaaaacca gcaaaatatc cagaaagtac tcctccgcct cctggctcag tagtaagccc
      301 tgccaaatct tttcctccta aaattgggaa taggatagcg aaagaatatt gatttatact
      361 tgatgaacct ggttgtaatg ctactgaaac tgatatataa tacacaccat tttcttgtac
      421 tgtaaacgta ccatttgcat ctactgttat acttgaacta tttcctatta ttgtttgcca
      481 acctgttata gcatctccat tatttagtgt ttgtggagat ggtgcattta cgaggaagtt
      541 agcaactggc tgagaggaat tgcctgttgc tcctgttgga cctgttgggc ctgttactcc
      601 tgtcgctcct gttgctcccg ttggtcctat acttcctgtc gctcctgttg gacctgttac
      661 tcccgttggc cctatacttc ctgtcgctcc tgtatttcct gttggcccta tttctcctgt
      721 cactcctgtt gggcctgttg ctcccgttgg tcctatttct cctgtcactc ctgtatttcc
      781 tgtcggaccc gttgctcccg ttggtcctat ttctcctgtt actcctgtat ttcctgttgg
      841 acctgttgct cccgttggtc ctatttctcc tgtcgctcct gttgggcctg ttgctcccgt
      901 tggtcctata cttcctgtca ctcctgttgg gcctgttgct cctgttggtc ctatttctcc
      961 tgttggtcct atttctcctg tcgctcccgt tggtcctata cttcctgtca ctcctgttgg
     1021 gcctgttgct cccgttggtc ctatttctcc tgttggccct atttctcctg tcactcctgt
     1081 cgctcctgtt ggacctgttg ctcctgttgg tcctatactt cctgtcactc ctgtcgctcc
     1141 tgttgctccc gttggtccta tttctcctgt tggtcctgtt gctcctgttg gtcctatact
     1201 tcctgtcact cctgttggac ctgttgctcc cgttggtcct atttctcctg tatttcctgt
     1261 tgggcctgtt actcctgttg gtcctatact tcctgtcact cccgtatttc ctgtcgggcc
     1321 tgttgctccc gttggtccta tacttcctgt cactcccgta tttcctgtcg gacctgttac
     1381 tcctgttggt cctatacttc ctgtcgctcc tgtacttccg gttgggcctg ttactcccgt
     1441 tggccctata cttcctgttg ctcctgtatt tccggttggg cctgttggtc ctatgcttcc
     1501 tgtcgctcct gtacttccgg ttgggcctgt tactcccgtt ggtcctgtac ttcctgtcgc
     1561 tcctcttggt cccattggac ctgtaattct aattggaaga cactcacatt taggataaca
     1621 attatctata attctatctt caaatgttac aagtatatac ggtttgttac aacctctact
     1681 agatgtaaat tcaagaatct gataagggta atttaaagat aaagctaacc cataattagg
     1741 atatttattc attgcccatg cttttatagt tcccgtaata tttaaacaaa tatagttgga
     1801 tgtatcactt atcccaactg gtaaatatcc cctaatattt tccatagaca ctctatcatt
     1861 ccatgttata gttcgtaaat tataatattc acttattctt ttaatttcaa tatctacttt
     1921 ttctttaaaa atatgattct tatttgaatc aatatgaata aataactctg cttttaatat
     1981 acttttgtct tttaatatat ctggtattct cactttaaaa aaagtaatca acttttcttt
     2041 tacaaaacta tttgaacatt tacttcctat taagcaataa tctaaattac taaagttttt
     2101 atctggatat tttttagata taaaagtatc atcatttaaa taaagtataa tatttctcat
     2161 ttataatccc tccattttgg gtctatatca tactatgttt aaaatacatg cttagttaca
     2221 atataattaa aatttatagt attaaatttt tatataataa tgctgatttc tacaaaaaaa
     2281 gaacatgtca ttagaaattt ctaatagcat gttctttttc attattatat attacacttt
     2341 aaattattcc ttgactggct tagctggtgt aaactaaacc agtctttcta aaatattaag
     2401 cacttttttc ttattatgtt ttttaggatt tattgtctat attattgtaa accaaagtta
     2461 taaaattgta aatagaatat attaccataa caatatcttt ttataagtaa aaattataaa
     2521 aaaccttatt tttattaaat agaataaata aaataagttt aaaaaatact gaaataagtg
     2581 ttttccacat attttatata
//
LOCUS       DAELTA010000264         2529 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.269, whole genome shotgun sequence.
ACCESSION   DAELTA010000264 DAELTA010000000
VERSION     DAELTA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.269"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            146..1771
                     /locus_tag="KPG13_003528"
     CDS             146..1771
                     /locus_tag="KPG13_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG0987210.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            1970..2446
                     /locus_tag="KPG13_003529"
     CDS             1970..2446
                     /locus_tag="KPG13_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987211.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
BASE COUNT         1019 a          247 c          475 g          788 t
ORIGIN      
        1 atttacaatt tttagtatta ttttaataaa tatttcacaa atttagtata aaaaatatac
       61 tatttacgtg ataatatata ataaaaatgt tatcaaagtt ttatgataaa ataaattatg
      121 agcaaaaaga aaggaaaata tattaatgtt acaagttaca ggtgttggac ttagatttgg
      181 tgataaggaa ctatttaaag atgttaactt aaaattcact aaaggtaatt gctatggaat
      241 aataggagct aatggtgcgg gaaaatctac ttttttaaaa atattatcag gagaaataga
      301 gcctaataca ggtagtatat ctataactga taaagaaaga atgtcagttt taaaacagga
      361 ccactttgaa tatgaagaag aaactgtttt aaatgttgtt ataagaggtc atgagagact
      421 ttggaacata atgaaagaaa aagatgcatt gtatatgaag gaagacttta atgaagaaga
      481 tgggataaaa gctgccgaac ttgaaggtga gtttgcagag cttgacggat gggatgcaga
      541 aactaatgct gagaaaatac ttatgggtct tggcataacg aaagatatgc attacaaaca
      601 aatgaaggaa ctagttggtg gggaaaaggt taaggtattg ttagcacaat cactttttgg
      661 taagcctgaa atactattaa tggacgaacc tacaaaccac ttagatttta aatcaataaa
      721 ctggttaaat aattttataa tggatttaga agaatctata gttataatag tatcacatga
      781 tagacatttc ctaaatcaaa tatgtactaa tatagttgac gtagattttg gtaaaataca
      841 aatgtacgtt ggaaactatg atttctggta tgagtcaagt caattggctt tacaattggc
      901 taaggaccaa aataaaaaga ctgaagaaaa gatagctcaa ttaaaggaat ttatagctag
      961 atttagttct aatgcttcaa aggctaaaca agcaacatca agaaagaaac agttagagaa
     1021 attagaagtt gaagatatac aaccatcaag aagaagatat ccatatgtag gatttacacc
     1081 tgcaagagaa attgggaatg aagttttaga agtacacaat ttaactaaaa ctatagatgg
     1141 ggttaaggtt cttgataatg tgtcatttag attggataga gatgataaag tagtatttat
     1201 gggagatgaa atagctacta ctgcactttt aaacatagtt atgggagaac ttgaaccaga
     1261 cagtggagag tataaatggg gagtaactac ttctcaagat tatcttccaa aaaatcataa
     1321 taagtttttt gacggagtag aatattcttt ggttgactgg cttagacagt tctctgaaga
     1381 aaaaagtgaa agttttataa gaggattttt aggtagaatg ttattctcag gtgaagaagc
     1441 attaaaagaa gctcaagtta tatctggagg agaaaaggtt agatgtatgc ttagtaagct
     1501 aatgctttca aatgcaaatg tacttgtatt agatgaccct actaaccact tagacctaga
     1561 aagtataact tctgtaaata aaggacttga aaagttccca ggagtgcttt tatttacttc
     1621 tcatgaccat gaatttatat ctactatagc aaatagaatt atagagataa ctccaaatgg
     1681 aataatggat agaaaaatgg actttgatga gtatttagaa agtaaagaga tacaagacca
     1741 attagctaaa atgtatggaa aagataaata attattagta taaaaccttt taatatttga
     1801 gacatgctat gcatgagtgt gtaaattttt aatcaaatat taggaggttt tttatattgt
     1861 ttaaacatgt ttattagcag aatagagtaa tattttgtat attaagaatt taaatataat
     1921 atagtagatg atataataaa aatatataac attttggggg gaatagatta tggttaagaa
     1981 aaggttagta gctgtagtaa ttagtttagc tatgattagt gtagggaatt tttcatatgc
     2041 tcactcagga gggcataatt catcaggagg tcatcataat aaaaataaat cagagttaac
     2101 acgttattat tgtggaggac attcagcaca tcaacacaat aaaggttttt gtccttatgc
     2161 tagtacaaaa gaaaattctg gagtaacaat atcttcagta cataacgaaa aaaatcataa
     2221 aaactatttt gcagaaaaag ggtataataa aggttatgaa gatgggtgta aagggcttta
     2281 taatttaagt gtcacagaag ccaaatctga taatttatat gaaaaagaga ttttaaaaag
     2341 ttcatatgaa aaaggatatt taattggata caatgaatat aaagcaaaaa aagtagatga
     2401 tgtaatgaag aagatgttag atattccaat gttagcaagt atataataat atagtattta
     2461 aaaagatatt ttctataagg gaatatcttt tttattgtaa agtataattt ttcgtaaaaa
     2521 agtataata
//
LOCUS       DAELTA010000265         2527 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.270, whole genome shotgun sequence.
ACCESSION   DAELTA010000265 DAELTA010000000
VERSION     DAELTA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.270"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            51..542
                     /locus_tag="KPG13_003530"
     CDS             51..542
                     /locus_tag="KPG13_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987212.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            570..1517
                     /locus_tag="KPG13_003531"
     CDS             570..1517
                     /locus_tag="KPG13_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987213.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            1807..2349
                     /locus_tag="KPG13_003532"
     CDS             1807..2349
                     /locus_tag="KPG13_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0987214.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         1114 a          201 c          369 g          843 t
ORIGIN      
        1 aatttttatg aatagtatta atatatagat acagggagat tgatgcgatt atgtatgaaa
       61 ataataaggt aatatacaac cttgaaagat gtgaaaaact agaaaagact ctttatttat
      121 ctcaatttaa tacatataaa aattttaaat tagcttctaa aaaaactgta ataaattcat
      181 tagagacata tgtttacaat atgaatatta acaatataag tcttgttgat actatagaat
      241 gtatatcatt agaagatgaa caacttacag gaaagaagtt gattttagtt ggtgatatag
      301 atattgatgc aatattagat tatgcaggca ataaaagaaa tagaaattct agtaaaaaaa
      361 atacatttaa attaaaaata ccctttagta catttataca aatgcctaga aagatagaaa
      421 ataaagataa aattaattta aagtatctta tacaagatat tacttcatct attttagatg
      481 ataatttatt tattagtgtt actgcaatta taagttatga aaatagttcc aagatagagt
      541 aatctttaaa ttatagaaag gtggatgcaa tgagaataac gaaaactact tttaatgatg
      601 tattttcaaa tgatgcaaat ggaagtatta taaaagtaga aaaatatgtt gataaaaaat
      661 gtgcatctgt atttgagatt ttgacctaca ctattattgt tacaaataca agtaggtaca
      721 aaactggaaa tatatttttc aaagattata tttctaaata tatagagttt ataaataata
      781 cggtaaaagt taatggcatt ataaaacgtg gattagaccc acagcaaggt ttttacatag
      841 ggagaataga tgcaagttgt aaaaaaataa tatcttttaa gagtgtagtt ttaccaaatt
      901 ctgcacatag gattataaag aatagtgcta atatatacta ttattataag tgtaatctag
      961 ataaattccc aacaagaata tcaatagaaa gtaatagagt ttatactaat gtaaataaaa
     1021 ttgtattcaa acaattgaat ataagtaata ctttaaaagc tcctaaaaat ctaagagata
     1081 tattaaaagt aaatgtaaat agtaaaatat ttgatgttaa acctatgaaa aatcttataa
     1141 acaaaggtag taatttgaaa ttatgtaact tgatagtttt tgggtatata gaagttgaaa
     1201 tagactatag ttgtaaaaat aggagtaatt ttaaaacagt aaataagtct aatgacatta
     1261 aaaaagataa gtattataaa aatgatatac aaaataacaa tgaaaatata aacgattgta
     1321 aaaagcaaat aagttttggt gataataagg taaaaaaaat aacaaaaact tttggatttt
     1381 cttgtttcat ttgttctcca gttggaatta tttatgaaga tatgaaaaat ataaatataa
     1441 aaattgaaca tacatcaata aatgaattaa atccagaaga attatttgta aatacatcac
     1501 ttttattgta ttattaagta ttttaaagct aaaacttaaa cttattaaaa tagcatttgg
     1561 ttaaaggaaa atagatattg atatatttat catatatata aatgattaat taaacaatta
     1621 taaataataa gtatagtata atatttaaaa aatatagatt tatgataaaa taagacaaat
     1681 atgttaaaaa aatgataatc acggagaact tttaccttct tcaattaata tattattatt
     1741 agtaataact ttaaaattat attactatta tataaaaatt ggcttaaaag aaggtgaaaa
     1801 aagtaaatga aaatatgcat aacagtaggt catagtatac taaaaggtgg taaaagtaca
     1861 ggtgtaaatg gtatagttga tgaatacaat tataataaaa aattagctcc tatgttagct
     1921 gagatgctta taagtcaagg taatacagta gatgtaataa tttgtccaga aaggcacttt
     1981 atgtcagaaa gagaagaatt tttctataga gttccaaaag taaacagtgg taaatacgat
     2041 ttattagtag agctacattt aaataaagct gatggaacac aatgtggtac agaagtgctt
     2101 tactatggca ctgaaggatt agaatatgct aaaagagtat ctaatagact tggagaatta
     2161 tttgaaaata gaggagctaa aaaaagagaa aatttatata tattgagaaa tacaaatcca
     2221 gttgcaatac agattgaaag ttttttctgt gataatgtga atgattgtaa caaagcaaat
     2281 gaatcaggtt atgattatat agcacgattg attacagaag gtatattgaa taaagatata
     2341 aatatgtgag attatggtga tttaggtatt gattaatacc ttttaatatt agatataaat
     2401 aaaaatacaa tgtattagta taatttaaac tttcaaaaga tagattagtt tgattattga
     2461 caagagatac ctccttgaaa atattgattt atcagtatat ttaaggaggt atttaaatat
     2521 aaaaatt
//
LOCUS       DAELTA010000266         2518 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.271, whole genome shotgun sequence.
ACCESSION   DAELTA010000266 DAELTA010000000
VERSION     DAELTA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.271"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            210..527
                     /gene="trxA"
                     /locus_tag="KPG13_003533"
     CDS             210..527
                     /gene="trxA"
                     /locus_tag="KPG13_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0987215.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(734..2008)
                     /locus_tag="KPG13_003534"
     CDS             complement(734..2008)
                     /locus_tag="KPG13_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG0987216.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
BASE COUNT          935 a          311 c          343 g          929 t
ORIGIN      
        1 tataatttaa gggaaaaaat gtcaactata ttaagaaaaa aataaaatat attagaaata
       61 tttagtctta taaatattta atcttataaa tctatttatc ataatattaa atctattaaa
      121 aataggcggt tacaaaaaag tgcctaattg aactgttata taggtcgtag tatattaata
      181 tcaagaagat aataaaggag atgtatatta tggtaaaaat agtaaataat aatgaattta
      241 taaatgaagt agaaaataaa gatggtttag tagttgtaga tttctttgcg acttggtgtg
      301 gaccttgtaa gatgctatca ccaatatatg aagctttagg aaatgagatg gttgaaaaag
      361 caaacttctt gaaggtggac atagaccaaa gtatagaatt agctcagaaa tttgaagtat
      421 ctacagtacc aactatgcta atatttaagg atggaaaacc tgtagataga ttaataggtt
      481 ttatgcctaa ggagaattta aaaaacaaaa ttgaatcata tatgtaatta aagtaaattg
      541 aagtaattaa aataaatctc atatataaac aaaaaaactc attgtaatga gttttttgtt
      601 tgtatagtgt aaaatagttt gtatagttac aaatgagtgt ctaattgcta atgaaattga
      661 ctgttgatat aatttgcttt tttgtgaaaa caaaactact ctctatttat tgataaaaaa
      721 gaatagaaaa aacttattta aaagccaaag ttttaatagc atgtataaag ctatccacct
      781 catcaaaagt attgtacatt cctaagctta tcctaacaag acctacagga ttttccctca
      841 taaatctgac atatgcttct tttggactta tacctaataa tttttgtaca taaatatctg
      901 cacctactac tccagctcca gtttctattc caaagttatc aactaaatat tttgctacat
      961 ctttgtaata catattactt atattaaaag atatatttgg gataattaca ttatttgatt
     1021 tatctccata tattattata ttaggtattt tactcatttc ttctttagca tataaaaata
     1081 attctctttc gtattttgaa attctgttta gaccaatttt atttaaagtg ttcagtgctt
     1141 ttcccattgc aagagttcca aaatactctg ggtatcctgc ttcaaaacgt tggggtacgt
     1201 ttgcatatag tattttgtta tcatttacaa atttggtaga acctgagcca tatagtacag
     1261 gattaaattt ttcaaagaat tttttaggtc ctaccagagc tcctccattt agtggagaat
     1321 aacatttatg agcactaaaa acaacaaaat ctatatgctc atcacattca tgtggcatca
     1381 ttgaaaaagg tttatgctga attacttgca caatatccac caatatcttt gcaccatact
     1441 tatgagctat tcttgctatt tcatatatag gagtggttat acctgtgaca ttactagctc
     1501 ctgtaactgt aacgagagat acattaccag cataattttt aagtatattc tctaattgac
     1561 ataaatcaag ttctcctttt tctgtaattc caacaacttt tgtaggaaaa ttaactttaa
     1621 aaggcaggta attagccata tgctccattg ctgtggtaat aacaaattta tctggatttt
     1681 catctttaaa aaattttgct aagaagttta ttccttctgt agcatttttg acataaataa
     1741 cagaatcctt ttcttcgtct ccatcaataa gtttgaggat agtagagcgt acattttcat
     1801 attttaaagt atttttttct gagattattc ctggagcatt aatataagta agcaaaggaa
     1861 tctcacagtt taagttatcc actacgtttt ttaaaactaa tggagtggca gagttgttaa
     1921 aatatattct tttaacagtt tcaccacttt tagttttaac tttaaggttt gcaccttcaa
     1981 tgtatttaga tacatccatg aattgcataa aaaaacccct tatctaataa actattctgt
     2041 tttttattaa aaaagctcaa gttttaatca agagcttgtt taatatatgt tatgttttta
     2101 taaggggttt tgttaattaa ttcttgttga gatgtttatt aaattacacg aaaatgagct
     2161 acagttgggg gaaactgtag ctcacttgat tttgggggag ataggttgaa cttattgttt
     2221 atccgtatca tatttttagt atagtcactt attttaattt atatacattt ttttataaat
     2281 tttacataaa atgttaaaca ctaattagaa ataattatga atatgactaa taaagttgta
     2341 aaaatagtaa agaaattgat tagaacataa tggaaaatat attgtatata attagaaaaa
     2401 aagattttat atgttataaa agatgtgcaa gtataataat tttataaaaa atacaaaata
     2461 taatacaaat aattttataa aaaaaattat aaaaaaattt aatttatata aattaaaa
//
LOCUS       DAELTA010000267         2500 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.272, whole genome shotgun sequence.
ACCESSION   DAELTA010000267 DAELTA010000000
VERSION     DAELTA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.272"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            127..2010
                     /locus_tag="KPG13_003535"
     CDS             127..2010
                     /locus_tag="KPG13_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG0987217.1"
                     /translation="MVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTE
                     SYEGTINYNKDPEYLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTV
                     NASQTRLKGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKNSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KENLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     DTGTTGKPLGGIREKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSL
                     RPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKT
                     RTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIED
                     SLK"
BASE COUNT         1036 a          300 c          418 g          746 t
ORIGIN      
        1 aatacaaaat tattaacaac agggattctt tcaatggcaa tcgtcgcacc tacaatggca
       61 tttgctactg aatctaatgc tatggaaaat aacgctgatt taaatataaa cttagagaaa
      121 aaaagtatcg ttttaggtag tacctcaaaa gtcagtgtta agtttaaaga aaaaccagat
      181 gcagatagca ttacattaaa gtataaatgc tatgatatgc cattggatac aactttaaac
      241 tataatcaat caactgagtc atatgaagga actatcaatt ataataaaga cccagaatat
      301 ctaaatgttt gggaactaca agggataaca ataaacagca aaaataatcc taaaacttta
      361 aacaaacaag aactagaaaa gatgggatta aatttaaaag actataatgt aacgcaggaa
      421 tgtataattg aagatataac ttctagaaaa gatgtaaata aatatttaag aaaaacttct
      481 gcacctatta cagaacttac aggaagtgat agatacgaaa cagcagttaa aataagtaaa
      541 gaaggctgga aaaatggttc agataaggta gttataataa atggggatgt aagtatagat
      601 ggcattatat caactccact agcaactaca tataatgcgc caatactttt ggttgaaaaa
      661 aacaatgtac ctaatagtgt aaaatcagaa ttaaagcgtt taaatcctag agatgtaatt
      721 ataattggag atgagaatgc tatttctaaa actactgcta atcaaattaa atcgactgta
      781 aatgctagtc aaacacgtct aaagggctct aatagatatg agacatcttt attgatagca
      841 aaggaaatag ataaaaatca tgatgtggaa aaagtataca taacaaatgc taatggcgga
      901 gaagtggatg cacttactat agcagcaaaa gcaggtcaag acaagcaacc aattatatta
      961 actgataaaa atagtattac agacaacaca tataaatggt taaagagtga ggatttacaa
     1021 aatgcttatt ttataggtgg ccctcaaatg atatcaacaa atgttataaa taaggtaaat
     1081 gatataacta aagataatgt tactaataat agagtatatg gagcagatag acacgaaaca
     1141 aatgcaaacg taataaaaaa attctataca gatgatgagt tagaggctgt tttagtagct
     1201 aaatcagatg tacttgttga tgctttagca gcaggtccat tggctgcgaa cttaaaatct
     1261 ccaatactta taacaccaaa gacatatgta tctgcatacc ataaagaaaa cttagaagct
     1321 aaatcggcaa ataaggtata taaaataggt ggaggattga cttctaaggt aatgagctct
     1381 atagcatcat cattatctaa acacaataca actccaacag aaccaggaaa tagtgggggc
     1441 aagacagtta tgattgaccc aggacatggt ggttcggaca ctggaacaac aggtaaacca
     1501 ttagggggta ttagagaaaa agactataca ttaaatactt cacttgcgac aactgaatat
     1561 ttacgttcaa agggattcaa tgtaataatg acaagagaca cagataagac tttatctctt
     1621 ggaaatagaa ctgctttatc taactctttg agaccagatt tatttacgag tatacactat
     1681 aatgcatctg atacaactgg gaatggtgta gaagtatttt ataagcttaa agataaagat
     1741 ggagggacta ctaaaactgt agctacaaat atattaaata gaatcttaga gaaatttaat
     1801 ctaaaaaata gaggtgcaaa aacaagaaca ctatcaactg accctacaaa agattattta
     1861 tatgttttaa gaaataatga tatgccagct gtacttgtag aatgtgcatt tttggataat
     1921 gagaaagata tgagtttact taatacatca aataaagtaa aagaaatggg tacacaaata
     1981 ggtaaaggca tagaagattc attaaaatag tctacaatga gagcatagaa aaatttaaaa
     2041 taaattaatc tgcaatttta aatttaaact aaagctataa gataagaatg taattataat
     2101 agtaataaag actgtatata cattgtgcat ataagtgcga aatgaggtat atacagtctt
     2161 tttatgatta attgtaaaag gatttatatt atatgaagaa tttacatatc ttaaggctac
     2221 agttaattgt caagagttgg agtattcatt tgtaatacac aataaaagct taattactat
     2281 attttattgt gtgtttgtgt gttttttgtg gatttttatg tcaataaatg atatagtata
     2341 gataattaaa tgtattaaat atatttaatt tttttatttt ttgagtttta taagtttatt
     2401 tttatatatt acttatatat tatttatatt ttgggttaga tacactttga catattttca
     2461 aaagttaaaa gattaaaaat aaagtagaaa tttatgttaa
//
LOCUS       DAELTA010000268         2487 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.273, whole genome shotgun sequence.
ACCESSION   DAELTA010000268 DAELTA010000000
VERSION     DAELTA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.273"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            82..399
                     /locus_tag="KPG13_003536"
     CDS             82..399
                     /locus_tag="KPG13_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987218.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            557..1516
                     /locus_tag="KPG13_003537"
     CDS             557..1516
                     /locus_tag="KPG13_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG0987219.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            1631..2416
                     /locus_tag="KPG13_003538"
     CDS             1631..2416
                     /locus_tag="KPG13_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987220.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
BASE COUNT         1009 a          195 c          369 g          914 t
ORIGIN      
        1 gatgttataa agtattgatt atattgacga ttatatgaaa taaactggat attataatta
       61 gaatttttag ggggtataaa aatgggcgtt ttaaaaggaa tgttttatgt atttttattt
      121 ttagatttag tgtcaatatt ctgtttcttt ttcaataaag gaaaaatagc atctaataaa
      181 attgtgttta atgcaatagg agtacttaca tttgtacttt gttttatgct ttttagttat
      241 tatcctaata ataatcttat tggaaaattt attgcttctc tattttttat atttggagtt
      301 gcaggagtta tgttgaaaga aaaaaatttt ttatatgcta gacttctttt aactgtagtt
      361 atagtttttt ctacattaag attgttcgtt attcaataaa atgtatttgt ataaaaattt
      421 attaaagtct acacatttaa gtgtaggctt ttttgatttt ttgtttgaaa attaaggaaa
      481 ttttaaggaa attttaaaat cgtcttaaat atttattgat atacttaaaa tattaatagc
      541 gaaaggagac ttatcagtga tagaaagtaa aaagtcagaa aaattgttac tgtcaattat
      601 atacataata tttttggtat atattttaat gattataaga ataattttat ttagagatgt
      661 accaatttac gctattttca agggaacgtt taggaatgta aatttaattc cattttatac
      721 aatatatcaa tttatagtag atagtaatct tgactttatg aaagctactg taaatattat
      781 agaaaatata ggtatattta ttcctatggg aattttttta cctatagtat gtaaaaatct
      841 aaataaaaaa actataatta tagtaattat acttgttagc ctagcatttg aattaacaca
      901 atatattttt gctcttggaa gtagtgatat agatgatgtt attttaaatt cattaggtgg
      961 cataattgga attaatattt acataaacat gaataaatta tttcccaatg atattaacag
     1021 atttaaggct ataataggta ctagtttagt tttaggagtt ataggattag gagttatttc
     1081 taaaaattat cataacttac taacttttaa atttagacct gatagaaaaa tatcaaaaat
     1141 attaattgaa gaaaataggg aaataattaa agatataaat aaagatacag tagatatagt
     1201 tgggacattt gagagcttta aaaaaggaat aataactatt aaagcaggtt ctaataacaa
     1261 agtaaagaga ccagagaatt taattgattc tgatggaaat ataagaattt atttaaatga
     1321 aaatacaaaa ttagtatctt atataattac caaagaatca aaaatggata tagttaaata
     1381 tgaggaattt gatgtgaaga atttagattt attaaggaga tatgatacta ttaatgtttg
     1441 gattgataaa gaaaatcagc caaaggataa acatggtatt atggctagta aattattaat
     1501 tggattgtat gaataaaata ttaaacaaga ataaaagaat aacaaatata aaaaacgaaa
     1561 aaactatttc tataattatt tttacgttgt gcataaaata agaacattag tgaaggagta
     1621 gagataaatt atggagataa taaaatatat cttgtacttt tttatatatt catttttagg
     1681 atggacagta gaaagtatag gatgttctat agcaagcaaa agaataatca acagaggttt
     1741 tttaaatggt ccaatatgtc ctgtttatgg ttttggagcg gtaattgtga tatcactatt
     1801 gggaagattt aataatgtag taatagtatt tctattagga atgattttaa ctacgatact
     1861 agaatatttt acaggattta ttttagaaac cttatttcat gctaaatggt gggattattc
     1921 tgacagaaaa ttcaatataa agggtagagt atgtttaaaa aatgcaatat actttggtgt
     1981 aatgtctgtt ttaataataa gatttataca tccatttata aaatattttg taagtataat
     2041 tccatataga atattaattt caatggctat aattacaaca ctttggacta tacttgattt
     2101 gattgttact attataacac ttaaaaaatt agatataaag ttaaacttac tagacgatat
     2161 aattacagac cttaatgata taaatgtgaa attggataaa tttgatagag gagaaataca
     2221 agctttattt aaaacaataa ataacgacga attagaagtt agagaaaaaa taaacaaaat
     2281 aaatagtaag ttggaccgta tagagtctaa tattatttta caaaaaagag ttataaaagc
     2341 ttttccacat ataaaacata aaaaacaaca agagcaacta gaacatttta aaaaaattat
     2401 taatgagaaa aggtaattat acaatttaat gaagacgaat agaataatgg tatttataag
     2461 atgattttca gaggactcta taaaaat
//
LOCUS       DAELTA010000269         2429 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.274, whole genome shotgun sequence.
ACCESSION   DAELTA010000269 DAELTA010000000
VERSION     DAELTA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.274"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            319..2214
                     /locus_tag="KPG13_003539"
     CDS             319..2214
                     /locus_tag="KPG13_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG0987221.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1062 a          244 c          446 g          677 t
ORIGIN      
        1 taatttagtg atatatattt aagtaataag agtaaagcaa aaattagtgc atagtagtat
       61 ataaaaatgg agtttgaata cttttgttag tatatgattg tttagtagaa aatcactata
      121 aaataggatt tctatctttg agcataatta tagagagcta agcaggggga aggggttcaa
      181 gaagatatta ttagtgtttg tgttagtaaa ttatgtaaac acataaaata tattgaaaaa
      241 taatattata tataatatta tttatgtgtg ggaaatacga atataaaaaa taaaaaataa
      301 tattcggagg acataaatat gttaagcaac aaaaagagaa gtatggcaat agtaatggca
      361 ggtgctacag ttatgagtgc agcagcacca atattcgcag acaatactgt gacagaaaat
      421 gttgataaaa attacacagt aagtgcgaaa gattctgcta aactaatcga agaggttaga
      481 aaagcattag aagttaagtt tgaagataca aaagcaggag caaatgtaaa tgatagagtt
      541 tacgatataa aagtagacaa tgtaaactta actaatgcaa ctcaattaca aaataagata
      601 aactctttaa cagaagggca aagtttaaaa gttactattc aagataaagg acatcaagtg
      661 ttaggtggaa aagtagttga ctataaaatt gaaaactaca aaactgctca agaaatagta
      721 gatgcagtta atgcttacaa tgcaacttta gcagaagatt ctgataacaa gttaacagca
      781 actataaaat ctacaaatac agttgaagta aaaagagcaa aagattcagc aaatgttatt
      841 acattaaatg taggagacca acatttagat ttctcaaaag tgataacatc agaagaaggt
      901 acttttgaag gttatgaaaa gagttatagt gacatagatt caaaagaatt acatacgata
      961 acagttaaaa acgcagattt acaagacata tcagcagaag aattatttga tggaattaga
     1021 ttaactacat taggaagaga aatagttaat aaagttaaaa atggatatgc tttaacattt
     1081 gaaaatgaag caatccttac tcaagaacaa gaagatagtg atgataaaga taaaccagaa
     1141 aaatcaagct ttgatatagt tttaagcaag gcaaatgaaa agccagaaac aataagtgtt
     1201 tcaagtaaaa accataagtt agttagagac ttacacaaag tattaactga tgtaaaaggt
     1261 ggaaaagaat taaaagtaga agttttatct ggtgattcaa gatttacaac agcagtagaa
     1321 gttagtaaag agagattcaa agatggagaa gcagaagcta taattttagt tggagaagat
     1381 gctatagttg atggattagc atcagcacca cttgcctctc aaaaaaatgc accaatatta
     1441 ttatctaaaa aagattcact accatcagaa atagaagctg aaatattaag agtacttgga
     1501 agtaatctat cttctaagaa aatatatata gtaggtggag aatctaaagt atcaaaagaa
     1561 actgaagaaa aactttctaa attaggtgta agtaaagttg agagagtttc tggagaagat
     1621 agatttgaaa cttctttaga aatagcaaaa caattaaaag atacatttaa gactgctttt
     1681 gtagtaggtg gaaatggaga agctgatgct atgagtatat cagctagagc tgctcaattt
     1741 ggtgctccaa taatagttac aggtaacgaa ttagatgcaa atgctgaaaa attattaaaa
     1801 ggaaaagaat tagaaatagt gggtggagaa aattctgtat caaaagaagt tgaagacaaa
     1861 ttagttgata tagatttaaa taataaagtt gaaagattag ctggagaaaa tagaaaagat
     1921 actaatgcta aagtaatcaa taaatactat gcaggtgcaa ctaaagcata tgtagcaaaa
     1981 gatggttatg taggtggaaa tggacaatta gttgatgcac ttacagcagc accacttgca
     2041 gctagttcaa aggctccaat agtattaact acagaagaac tttctaaatc acaagaagaa
     2101 gtagttgagt taagacttaa gaatgctact aagttagtac aaataggtga aggaatagct
     2161 aaaaacgcta ttgaaaaaat agcagaaaaa ataaacttat ttactaagaa ctaataagat
     2221 ataggaatta atattaaaag tttgtttaga taatatatga ttatacaatg cgttaatatg
     2281 taagacatag tgtagatatt ataaaatata caaataacaa gataaaataa taaaaataag
     2341 gtgtctcatt tagaattaga ttgagatacc ttattttatt ttttgattat aatgttgaga
     2401 aattcatatt gcaaaaatta ttataaaaa
//
LOCUS       DAELTA010000270         2427 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.275, whole genome shotgun sequence.
ACCESSION   DAELTA010000270 DAELTA010000000
VERSION     DAELTA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.275"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            44..643
                     /locus_tag="KPG13_003540"
     CDS             44..643
                     /locus_tag="KPG13_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987222.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(813..1280)
                     /locus_tag="KPG13_003541"
     CDS             complement(813..1280)
                     /locus_tag="KPG13_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987223.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            1391..2335
                     /locus_tag="KPG13_003542"
     CDS             1391..2335
                     /locus_tag="KPG13_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG0987224.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
BASE COUNT          925 a          278 c          386 g          838 t
ORIGIN      
        1 ttaaaatttt ttatagtata aaacatctag gagggtaata aaaatgaaat taaaaagaac
       61 tttatttgta ggattagcac tagtattagc tataggtata actgcatgta ctaaaaataa
      121 tgaaaaacca agtgaaaata caggaaaaaa tcctaatgaa tctgtaaata ataatgaagg
      181 atatatgaat tattattcaa caagctataa tgattatata gcaggtctaa atagatattc
      241 tatttatgat actccagaaa gtataaataa tcagtttaaa gataaagaat atccaggaaa
      301 tgaaaaatat ctaagtgatg taaaggctgc atataaagat agtagagata aaatacaatc
      361 atttgtaaca tctcttaaaa aagatggtaa aacagaagat acagagttaa agaaaatgaa
      421 tgatgaccta atcgctgaag gggaaagatt agtaaaagat atagataaga gaatagaaaa
      481 attagataag gtatctgatg aagatatgaa aaaaggtcaa aatgacttta taaaattagt
      541 tcatggaact gaagatgttg gaaatgatat aggtagtggt tttagaaaaa tgataaaaga
      601 tatgaatgat agattaggaa taacaggaaa taataagaaa taatagaata aatagttttg
      661 aaatttgaaa taaattattt gagatatagc gaagccttat aaataataca ttttttggct
      721 tcgctttttt gcatgttatt aagatattaa tttttatctt gtaaccttaa tatttttatt
      781 ataatttatt tagaggttat tttcacacgt ttttacttgt tttttctctt tttaaatttt
      841 tgttttgcaa aattgtaaga ttctcctatt aaagttttaa agttctcaaa tgtcttttca
      901 gtagggctta ggacacatat ccatcccatc catgagtaga taggatgtgg catgattgtg
      961 tccaattttg taaaatcaaa atccatatca actatttgac ctacacctgg acgctttgga
     1021 atatatccaa acatttcaat aaaagtttct ttttttaatc ctatattcac acggtataca
     1081 tttggacgac taactaacga acctttatca ttattactgt ctttttcctt aatggtaaga
     1141 acatataccc ctcgctttaa aacaccatta ggattgtagt aaattgctcg ttccccccaa
     1201 ctatccatta gtacaacatc atctaagttt gatagacaat aattgagaat atcatcagcc
     1261 ttaatagtat cattttccat aaagtatcaa tcccttcttt ctttactatt attattgctt
     1321 tagatgcaag aatatcttat catataatag tgacaattat tgtcactata tttaaattag
     1381 gagtataaat atgtcaagag ctgagcgttt aattgaactg atgataacaa taaatgcaaa
     1441 aagaagtttt acagcaggag aattagcaga agaattttct gtatcaaaac gtactatact
     1501 aagagattta caggtgctgg agagtattgg atttccatta tattcaaaag ttggagcggc
     1561 tggtggatat catgtactta aagagcgtat acttccaccg attgcatttt cagaaagtga
     1621 ggtaaaatct atattctttg catatcagtc actagaatat tataatgatt tgccatttga
     1681 gcaagaaact atttctgttt taaaaaaatt tttaaattgt ataccaaatg atattcaata
     1741 taatattgag aatataaggc gtaaatttgt tttttggaca ccagataggc actgttcaac
     1801 accattattg aaagaattgt ttaatatagt aatgaatgaa tttacaatta aaattgaata
     1861 ttcatcaaag caaaaaaata gtgtacgtac cattgttcct attggtttgt atgccatgaa
     1921 tggactttgg tattgtccag cttactgtat aaaatcagaa tctattaagg aatttcgtgt
     1981 agacagaatt gtgaagattt tgagtataga aaacttatca agtaagaaat ataaggtttt
     2041 atcttctata catgattatc ttaagaatat ggaagtaggt acagattatc atattaaaat
     2101 taatttgaca gatgagggag taaaacgttg tgaaacagaa tttttgttgg caagagggtt
     2161 gaaaatcttg tctaaaggtg gatacataga tatgtatata ccaaaatcta cacttaactg
     2221 ggtggctgaa tattttttaa cttttgggaa aaatgctaca ataatagaac ctatagaact
     2281 taaacatttg ataaagtcta aagtactaga gctatataat catcattgta tttaaattat
     2341 tttatttaat agtaggatag agtttggata aaatgttaat tttcttaatt tactttacag
     2401 atgatttaga tagattgatg ttattta
//
LOCUS       DAELTA010000271         2416 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.276, whole genome shotgun sequence.
ACCESSION   DAELTA010000271 DAELTA010000000
VERSION     DAELTA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.276"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            89..1363
                     /locus_tag="KPG13_003543"
     CDS             89..1363
                     /locus_tag="KPG13_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG0987225.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            1481..2200
                     /locus_tag="KPG13_003544"
     CDS             1481..2200
                     /locus_tag="KPG13_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG0987226.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
BASE COUNT          986 a          237 c          453 g          740 t
ORIGIN      
        1 attttaaaaa ttatagtaag aaaaatgtaa gtatttgcaa tattaatggt ataatattta
       61 cagatatgat aatcaataag aggaaaggat gtttataatg gaaaatgatt taacaatgca
      121 agaattactt gaccagcaag aacaagtatt cagcaaagtg aaagttggag aattgacaac
      181 tggaaaaata actgcagtga gaaatgatga agtacaatta ggattagact atgggtttga
      241 tggaataata cctataagtg aattgaacat agaaaaaaat caatatatag aagatatata
      301 ccacataggt gatgaaataa ctgctgtaat aacaaaagta tctcaaaaag atggtacaat
      361 aactttatct aaattacaac ttgacaaaag aaatgatttt gccgagttac aaaaggctta
      421 tgatgaacat agaataataa ctgtaaatgt tgaaaagaat atagataaag gtgtatttgc
      481 taattacaat acatatacat tttttatacc aatttctcaa cttgatacaa aatttattac
      541 agatacatct aaatttgttg gtcttaactt agaagtttat ataaaagaat tggatgttag
      601 aaaaaataga cttgtagcat ctcatagaga tgtattacaa gagcgtataa ataaagagag
      661 agaagaaaga agagctcaaa ttaaggcaga aaaagaagct gaaagagcaa gaattaagca
      721 agaaagagaa gaagaaaaag ctcgtatcaa agcagctaaa gaagacttat ttaattctct
      781 tgaagttgga caaaaaagag atggtaaggt gacaaagata atgccttatg gtgcatttgt
      841 ggatattggt gggattgaag gattagcaca tataaataat ttagcttgga caagagttga
      901 aagtgttgaa gatgttgttt cagagggaca agaagtagaa gtttatgtgt tggatgtaaa
      961 taaagagact aaaaaaattg ccttggcatt aaaagacata aataatgacc cttgggattt
     1021 aatagcaaaa gaagtacaaa ttgatgatgt tgtcaatgtt aaagttctta gaataataga
     1081 aaaaggggct tttgttcaaa ttaaagaagg tgtagatgcg tatttaccaa tatctgaatt
     1141 gagtgatact agagtggcta aagttactaa tgttgttaat attggagatg aggttaaagt
     1201 taagattctt gattttaaac caaagactaa gagaatgttg gtttcaataa aagaagcaac
     1261 tagagagcca gaagaggata taacagaata cctagaagta gaggaatctt taggaagtat
     1321 tggagaatta tttaaagata aatttaaaga tttagaagtg taaaataatt gaaaatgggg
     1381 ctgttttaaa acgttacact gtctattatg acagctttag ttttgaaata gccctttagc
     1441 tattttcacg aatttatgta aaatattagg aggaaattta atgaaaaagt ctattatgga
     1501 tgtacattgt cacactctaa taagtggaca tgcacatagt actttcaagg aaaatgtcga
     1561 agaggcgagt aagaagaata taaaatactt aggaatatca gaccatggac ctaatatgcc
     1621 aggaggacca catccattct atttttataa tttgcatttg cttccacgtg aagtgcaagg
     1681 cgtaaagatt cttagaggaa tagaaggaaa tataatggat tataatggta atttagatgt
     1741 tcaagaagat atgttgcaac atttggatta tataatcgca agtttgcata gaccttgtat
     1801 agcttctggt acaaaagaag aaaatacaaa tgctatttta aaagttatgg ataaacctaa
     1861 ggtaaaaata attggacatc cagatgacag taggtatcct ttagattatg aacctatagt
     1921 aaaaaaagct aaagataaaa atatcctttt agaaataaat aattcatcat taagttcaaa
     1981 ctctcataga acgggtactt gggaaaatgt aagtcatatg ttgacattgt gtaaaacata
     2041 tggtgcaaga gtaattcttg gtactgactc tcatatatgt tattctatag gagaatttga
     2101 aaatgcagaa aaagttttag aagcagtaga ttttccagat gaattagtca taaattatca
     2161 tgaagatgaa attatagaat tttttgatat aaatttttag aatcttggaa gtgaaaaaat
     2221 ttgagtaaaa ataagtttat ctatatgaat tattatgaca atggttattt taaaaaagga
     2281 atgactatat atatggtttt agcacttata attttagcta caatttttat atattcaacg
     2341 acaatattat atattttatg tataataaca gcagtttttc tatttaaaca aggtcgtgat
     2401 ttttcaaata aatatg
//
LOCUS       DAELTA010000272         2346 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.277, whole genome shotgun sequence.
ACCESSION   DAELTA010000272 DAELTA010000000
VERSION     DAELTA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.277"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            141..329
                     /locus_tag="KPG13_003545"
     CDS             141..329
                     /locus_tag="KPG13_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987227.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            589..1788
                     /locus_tag="KPG13_003546"
     CDS             589..1788
                     /locus_tag="KPG13_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG0987228.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1840..2343
                     /locus_tag="KPG13_003547"
     CDS             1840..2343
                     /locus_tag="KPG13_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0987229.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
BASE COUNT         1011 a          158 c          453 g          724 t
ORIGIN      
        1 ttaaaatatg taatgtcact gatagttaga atatttaagt gctaaaagat gtgtataaat
       61 aataatgatg tcagtttata atgatattaa attgagatat actgtttctc taaattaaaa
      121 aataaaggag taaatgtgtt atgaattatc atacaaaaga agaattggtg gaagcactga
      181 gagtagtatc ttcaagtatt attaattgtg gaaaaggaca gaagaaattt tctgaggaga
      241 catctcatca tacacgtttt aagaatatta tagaagtaat gtatatttca aaatcattaa
      301 taatggatga aattagtaaa agagattgaa gcttattttg caatttaaca tgttttaata
      361 tacattttat tatataagaa aataagtaaa catacatgtt ataaaatatt gaaataatat
      421 acaattataa atttgttaac attaatatct atatgttaaa atatagtaga agatgttagt
      481 aaataatagg atgagttcta ttttaataca attaaattaa gcttagtttg aaatttgtat
      541 ctaaaaagta aagtaatcta gaattaagaa aatggagata gaattataat ggaaaaatta
      601 tattatacaa atcagtatat aagagatttt acagcagaaa taattgaaat aaaagaagta
      661 gaaaataagt atcatgtttt attagataag acagcttttt ttcctggtgg aggaggtcag
      721 tttggagacc tgggaaaaat agagaatgat gatgtaataa atgtgtatga agatggaaaa
      781 aaaatatacc atgtattaga taaaaaacct ataaagatac ataaggtaaa gtgtagtatt
      841 gattgggata gaagagaaga tggaatgcat caacattttg gacaacatgt attgtctgga
      901 tgttttttta aaacttttaa tgcaaatacg acaggttttc atttgggaaa tgagtatagt
      961 acagtagata tagaaggtaa tttagaagag gataaaatta gagagataga gttgtttgcc
     1021 aatgaaatta ttcgtcaaaa tatacaagta gagacattac ttccaacgaa aaaagaacta
     1081 aaaaagattt ggttaagacg tgatttgcca aatactgatg aagagataag agtaataaaa
     1141 ataggtgatt tagatacgaa tgcatgttgt ggagtgcatc caaaatcaac tggtgaattg
     1201 agaatgataa aaataaagaa atgggaaaaa cataagtcaa gtacaagaat agagtttcta
     1261 gcaggtaaaa gggcagtgga tgaagtattg aagagggata tatatttgac taaaatatgt
     1321 agatatttaa gctgtagtga ggaagatgct ataaatggta ttgtaaatct aaatagtaaa
     1381 gtggaagagg ctttaattaa aaaaagaagg cttgaagaag tagtatctac atatcaggtc
     1441 aaagaaatga ttgaaaaagc aaataaggtt ggcaatattt cggtcattaa aaaaatttat
     1501 gatgatgaag atttgaaata tgtaaataag atagcaaata aaattgcaga atatgaaaat
     1561 agtattgcat tattagctgt taaatttaat gataaaataa atttagtttt tgcatgttct
     1621 aaaaatttaa aagatattaa tatgggagtt ttattaaagg atgcaataaa tcttatagat
     1681 ggcaagggtg gtggaagtaa agtattagct caaggaggag gtaagaataa tggaaattta
     1741 gaggctacac ttgattatgc atttatgaag atagaaaaaa ctctataaat atattatata
     1801 aaaaacctta gtagataaaa tagatatgga ggaataagta tggagcaaca aaaggactac
     1861 atagagttat atgaaaatga agaactaata gagataagag aagcacgtat ggatgatttg
     1921 gatacaatag caaaatttaa ttataatttg gctaaagaaa ctgaaggaaa agaacttgat
     1981 atggatgttt taactaaagg tgtaaaagca ttgttattag atgagagaaa aggaaaatat
     2041 catgtatata cagtatttga taaagttgtt gcacagatta tgtatacata tgagtggagt
     2101 gattggagaa atggaaattt cctttggatt cagagtgtat atgtagataa ggaatataga
     2161 agaaaaggta tctttaatta tttatttaat tatataaaaa atatatgtga taaagatgaa
     2221 aatatagttg gaatgagatt atatgtagaa aaagaaaaca taaatgcaaa agcaacatat
     2281 gagtctttaa atatgtatga gtgtgattat aatatgtatg aatacgaagt agtacattcg
     2341 taatat
//
LOCUS       DAELTA010000273         2330 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.278, whole genome shotgun sequence.
ACCESSION   DAELTA010000273 DAELTA010000000
VERSION     DAELTA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.278"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(86..1653)
                     /locus_tag="KPG13_003548"
                     /pseudo
     CDS             complement(86..1653)
                     /locus_tag="KPG13_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1989..2171)
                     /locus_tag="KPG13_003549"
     CDS             complement(1989..2171)
                     /locus_tag="KPG13_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987230.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT          732 a          316 c          259 g         1023 t
ORIGIN      
        1 aaaaatacct tataaagtta attttttaat actttataag gtaaatacat attgtattgt
       61 ggggataaat ttaaatcatt tgatactaat caacactaag aaccaataac tctctatcat
      121 cttgagtaat tgtatatatt cttaatttct tgtatgtcat aatattttct ccacctgtaa
      181 aataactcct aagattaaca caatcagttt taggttgatt tgtatctcct aaataattac
      241 gcaattcttt atctttagtt tcaatatctt taaatccaaa atattgtaaa gtagcttcaa
      301 ctctatattt ttggacataa tcgagagctt ctttattagt ttatgttggt gtaaaataca
      361 taccatttat ataagccttt attgtacttg gattaaattt aaaatctgca gaatatattt
      421 tatgtgtaac tattatttct tgaacagttg gaattttaac ttctggttca tcaagtattt
      481 caggagtact atttagctct gtaatagata agttatctaa gtatattgta tcactatcaa
      541 ttaatgctat atttatatta gaagaatctt tttcagtagt ttcaaattca taactaaatt
      601 ttgtatatcc ttgttctagt accaaataat ccgttttttc ttcttttact tttatattta
      661 caattattga attatatgtt aaaggatttt ttgaatttta ctaaaaacat aacgtttata
      721 aggttttagt tcagacatag gtattttaat cttcgtctca ccatttaatc tatttgctga
      781 gccttttaaa ccatcttgat ttgtagtatt aacattactc catatactag ggtaattatt
      841 gtaatcatca aaattagtaa agtaagttgg tgtttttata agtatattca ttcctctttc
      901 aagtttaaca ttacatattt gtttatcaga caaagttttt aaactgtctt taatcttatt
      961 gcctatatta tcatcaaata tgagttcaac acaactttca tctattggta tattattaaa
     1021 atataacaaa ccatcttttt tagtagtgcc aaaagttttt tcaattgctt ctccaattgt
     1081 aagttcaggt gttttatctt ctgggacctg taaactttta gcagtaattc ttcttttgta
     1141 agattcattt tctgtatcta atataataga tacagaaata ctgtcaattt gacctatatc
     1201 gtcttaccaa ctatttcatt ctgttactat ttgtccagaa ccattttttg taccaaaatt
     1261 tccacttact tgtgttgttt ctaatttaat ttgctttcca tcatctaatt tttttaatta
     1321 attataattt ataggaatca gtctagaact aaattgatac attgtgttaa gagccatagg
     1381 tgaaaatcct tttttgagat aagtatttcc aggagataga ttattgccaa tttgattttc
     1441 ttgtgctttg atagttgata atgcatctcc atctaacact aaatttgttg ttggtgtcac
     1501 tttgtacata ggtgcagtac ctgtgttgta gtatctaata tttgcattta tatatgcaga
     1561 ttctcctttg tttatactta atccagtatt tcatgattat ccattgctat tttgaacagc
     1621 agttgaatta tctgttgtat ggtaataatt tgtcttgtat agtttcttta aactcattaa
     1681 atttttctaa tgaaatttcg ttttgatttt ttgttcttat tactttatta aatgcaaatt
     1741 tttctggaga ttcaaaataa tatacataca taggtttatc tattttattt tttgatatag
     1801 catttcgaag ttctctatat tctttttctc aagaatttgc ttctatttga taatcataaa
     1861 aataatttct tgtctgagaa tatttgctta tctctataga atctttttta tatgatccta
     1921 atacttcttt ttcagatttt tcaagttttt gatcttttct ttctcattct ttagcctttt
     1981 ctttatcttt aaaaaaatcc ttctgatctt tctataggag ccttgtaagt agtattacaa
     2041 acttttccac tatagctttg taaatcttgt gcataaatgt tattaggtaa caaactacct
     2101 aaagttagat ataatatcaa caatatagat atacaattac taatactttt atgtttccta
     2161 aattttttca tttattctcc ctacaaatat tagtttagtt aacttttgaa ttttaattta
     2221 tttagattat ataatctaaa taagaatttt ttatataaat ttcataaaat atgtaggtat
     2281 atatcataaa aatatgtatt tatatgatat gtcagtacaa aataacaact
//
LOCUS       DAELTA010000274         2322 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.279, whole genome shotgun sequence.
ACCESSION   DAELTA010000274 DAELTA010000000
VERSION     DAELTA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.279"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            361..975
                     /locus_tag="KPG13_003550"
     CDS             361..975
                     /locus_tag="KPG13_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG0987231.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            993..1355
                     /locus_tag="KPG13_003551"
     CDS             993..1355
                     /locus_tag="KPG13_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0987232.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1461..1958
                     /locus_tag="KPG13_003552"
     CDS             1461..1958
                     /locus_tag="KPG13_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG0987233.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          896 a          259 c          349 g          818 t
ORIGIN      
        1 tttatcataa ttgcttaaat tactctttat cacctaatta tacaccatca aataggattg
       61 gtaaaatatt catctcatat aagtattact atataataat ttattctatt agtatactca
      121 aatcatgtaa aatttatata actcaattaa ttttttaaat acaactttat aatataaaac
      181 ttataataaa agtaaattta tatgttaaat gaggttgatt ttttattatt ttttatttat
      241 gctagtacta aagacttgca aacgtttttt gacaagtctt tattttgtct aaattaaaca
      301 aactttttta caaataaata cataaaattt aaactaatct atttattgga ggagataaaa
      361 ttggaagaag taattgcaaa aattattact atagaaaatg gattcaaaga aattgaaaac
      421 attgctaaaa aaattgtaaa aaataatgac tcaaaaaagt gttattatct atcaatctac
      481 ttatataggt cagaatatta tcaagtaaga gaattggctg tgtttatact tggttttata
      541 tcagtaacta tttctgaagc tttgttattt ttaaaagata atgtgagtaa agatgaaaac
      601 tggagagtac aagaaatact agctaaatca tttgattatt attgcagtga agtaggatat
      661 gaaaaggctc ttcctgttat aaaagaatgg cttaattctg atagtccaaa cataaaaagg
      721 gctgtgacag aaggtcttcg aatttggact agtagagact attttaaatc taatcctgac
      781 gttgctatat cactactttc tagtttaaaa gaagatgata gtgaatactt gagaaagtct
      841 gtaggtaatg ctcttagaga tataagtaaa aaatatccag atttagtaaa aaaagaatta
      901 gattcatggg atatatcaaa taaaaaagtt gagtttgtat ataagcttgc ttcaaaatgt
      961 atcttggcaa agtaaaatgg aggtgaactt atttgaaact tcgtgatgag tatacttgcc
     1021 cattagaact aacacatgat atgataaaag gcaaatggaa atctattata ttatggagat
     1081 tacgcatagg accaacttcc ttatcaaaac ttgaaaagga aatcaatggt attaatcaaa
     1141 agatgttact tgaacaactt aaagaattaa ttgaatttgg tcttgtagat aaaaagacat
     1201 atgaaggtta cccacttaaa gttgaatact cacttacacc tattcgaggg aaacaaatat
     1261 tggatgcttt ggctataatg caagatatag gaattagctt tatgttggat aatggtatgg
     1321 aagatattct taaggaaaaa ggtgttttaa aataaataga tacccacaaa aaagtaagta
     1381 atttaccatt attttcaact ggtatataat taaattatag atatattcta ttaatttatt
     1441 ataataggag gtaaattatt atgaaagtaa ctagtacagg tatagtaaat ggtgttatag
     1501 aagataaata cggtaaaaga ggaactcagt tcaatgaagg tggtatgcct acatattcac
     1561 tacctctaaa gtttgaagat gctccaaaga atacagtatc ctttgctgta tttttagaag
     1621 ataaagactc tgttcctgta tgtgggtttg catggataca ttggttagct gtaaatataa
     1681 caaaagatga gttaaatgaa aatgaaagtg taacagctac tgattttgta caagggacta
     1741 ctagttggca tggcaaacta ggaggtcttg acagattaga ggccagtgta tatggtggaa
     1801 tggctcctcc agacagacct catgtctatg aaatacatgt atttgcactt gatactatcc
     1861 ttgaccttga aaaaggattc tatatgaatg aatttttcaa agctattgaa gggcatgttt
     1921 tagatacatt tacactaaaa ggaagttatt ctaattaatt aggttgacta tagctaatca
     1981 ttaattaaat gctacattta attgatgatt agcttaattg ttattagaat attttctgca
     2041 ctttatctta atatatattt tagttgactg gtattttaat ttctattaga ttattatcaa
     2101 tttatataaa ttgattttag cttaaaactt acttatctat ttcagtttct attagaatta
     2161 ttattagact cattaaaata aaagcactaa tataactaat aatatatgtt aaattcatta
     2221 actctacata aagttttata atctaaatga aatacaataa tctgcataaa attcaatagc
     2281 ttatataaaa tttaataatt tatataaaca ttatcaatct at
//
LOCUS       DAELTA010000275         2315 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.280, whole genome shotgun sequence.
ACCESSION   DAELTA010000275 DAELTA010000000
VERSION     DAELTA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.280"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            1067..>2315
                     /locus_tag="KPG13_003553"
     CDS             1067..>2315
                     /locus_tag="KPG13_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG0987234.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGK"
BASE COUNT          904 a          294 c          316 g          801 t
ORIGIN      
        1 attttaaagg ataaaatgct gtataaatta ttggggtatt ttacctatac ttgttacaaa
       61 tttacatacc acttagttaa tataaaacaa atttcaaaga gattggacaa cgaataaaaa
      121 ttgcaattta tataccatat agttaatata aagcatattt gtgtcgatat atagttctaa
      181 ctaagtataa gatttacata cctcatagtt aatataaaac acatatttta catgtttgaa
      241 gattttatca gaaaccctat ttacataccg catagttaat ataaaactat taatagtgct
      301 atgttgttta tttgcattaa caagatttac ataccacata gttaatataa aacatgttta
      361 gcaggagtta tgatgataaa acacattatt tatttacata ccacttagtt aatataaaac
      421 cccaaaataa actaagtatt ttcagtactt acacacatac aactctctca aatttgcagt
      481 gaaccatgag tagtgcaatt gataacattt atcatacacc ctcaatccct tacattgcaa
      541 ctgttaaacc ctactttatc aaaaatatcg ctcactgcaa aatttatata tttttattat
      601 atcataaaat tcaaactata aaattaaaat ggaataattt atcaatttaa gaacacctac
      661 atatttgtaa atacttacta tattatctat tctaatagtt cttcttctag ctagaactaa
      721 tataaaaatc atctctgaaa gattaggaca tactaatata aaaataacta tgaatagata
      781 ttttcttgtt ttaaacgata tggataaaaa aacttctgaa aatataatca aagttctctt
      841 taaataacat tccattttat gtaagtaggc attatatttt tcttaaatgt cacttaaatg
      901 tcagttatat ataataatag taaacaactg tatttcacta atatttgcta ttatctaata
      961 gtatttatag tttagatttg atataataca tgtggtaaaa ttttgatttg taaaacaaaa
     1021 ttttacctac atatattaaa acaaatttaa aactaaggag acctatatgt taaaagtact
     1081 aaaaggaaat ataattttta caaaaacttc agaaactttc acagtatttg aaaatagtta
     1141 tattatgctg gaaaaaggta aagtaaaggg aatatttaaa gagataccta aggaatacaa
     1201 aaacctaaaa gtagtagatt acactgataa gcttataata cctggtatga atgacctcca
     1261 tgcccatgcc tctcaattta aaaatcttgg aatggctatg gataaagagc ttttgccatg
     1321 gcttgaaact tatacatttc cagaagaatc aaattataaa gatatagaat atgcaacaaa
     1381 aatgtataaa aaatttgtaa aagaactttg gaaatctgga actacaagaa tagcagtgtt
     1441 tgcaacagta cataaagaag cctctatgaa acttatggat atattcaaag aagttggtct
     1501 tggagcatac gttggtaaag taaatatgaa tatgaattgc cctgatactt tattagaaaa
     1561 cactcaggat tctattactg atacagaaga aattataaat aagtatagtg atacagattc
     1621 tattgtaaaa ccaataataa cacctagatt tataccaagc tgtacaagtg aacttttaaa
     1681 aggtttagga gatttatgtt taaaatataa tattcctatt caatcacact tatctgaaaa
     1741 ttatgatgag attgaatggg ttagaagtct agaacctgaa tctaaattct atggagatgc
     1801 atataataga tataatttat ttggtcaaac tcctacttta atggctcatt gtgttcattg
     1861 ttctcaggat gaaatagatt taatgagaga aaataatgtt atggcagttc actgtccagc
     1921 ttctaacttt aatgttggta gtggtgctat gcctatcaga aaattaatcg ataataatat
     1981 tagattagca cttggatcag atataagtgg tggacataca ttgtcaatat ttaaagcaat
     2041 ggtatctgca atacaattat ctaaattata ttgggttaac tctggtaaga agtataattt
     2101 cttatcttta tctgaggctt tttatattgc aaccaaaagt ggtggaagtt tctttggaaa
     2161 agttggtagc tttgaagaag gttatgattt tgatgcattg attattgatg attcaaattt
     2221 aaatcatgac aattattcta tacttgaaag acttgaaaga tttatatatg ttggtgatga
     2281 tagaaatatc attcatagat atgtatgtgg aaaac
//
LOCUS       DAELTA010000276         2283 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.281, whole genome shotgun sequence.
ACCESSION   DAELTA010000276 DAELTA010000000
VERSION     DAELTA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.281"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            654..1316
                     /locus_tag="KPG13_003554"
     CDS             654..1316
                     /locus_tag="KPG13_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987235.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1325..>2283
                     /locus_tag="KPG13_003555"
     CDS             1325..>2283
                     /locus_tag="KPG13_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987236.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGL"
BASE COUNT         1014 a          199 c          364 g          706 t
ORIGIN      
        1 atttttttag atattcaaaa agacattgta agttatatta ataatatggg tatcaacgaa
       61 ttttggatat aatgagtttt tcaacttaaa gttatgtaat aatgaatgta aagaatattt
      121 aatgaaatca ttttgttggt tatgtctagc tgaatacaat aatcttttag ataaagattt
      181 tatatttgaa gataggtgtg aaaaaattaa gaagatatta acatcagcag tttagaggca
      241 aagcatgtaa agtaaagtct gggtaactaa aagtagtaat ttagattgta actattgtta
      301 taaatatgaa aatgaggaaa caggaaatat aagttaggat gtagaaaata ggttactaaa
      361 taaaaagctt aattatataa tcaaagaaat aggtatatac tgtacaattt catggtgaag
      421 aacttttcct aaataaaaag gttggtaaaa gagatataag aaataaaatt caattataag
      481 atatggtatg ataactaaag gcactatatg gagtgaatag gcggaagaat tttttgaaaa
      541 ttataaaaat agtttctgtt gctacatatc aataagtata gatagagatt gcaaaatcca
      601 tagcaaaaat cgaatttata agtgtaaata acatgtaaga caaggagtaa attatgagta
      661 ttttagagat aaaaaaccta aataaaatat atggtaaatc ggaaacagag gtgaaagcgt
      721 taagtcaaat aaatctaaaa ataaatagtg gagaatttgt tgtgatagta ggtaaaagtg
      781 gaagtgggaa aagtacttta ctacacataa tggcaggact tgaaactcca acaaatggtg
      841 atgtgattat tgataatata aatatatcat ctcttgatga aaagaaaaga tctgcattac
      901 gtaaagaaaa gttgggatta atatttcaat catataattt gattccagtt ttaactgttg
      961 aagaaaatat aaaactacca actataaata taaaaaataa agatgaagct tatattaatg
     1021 aattgatgga gttactagga ataaaagata gaagaatgta tttgccaaat caattatcag
     1081 gtgggcaaca acaaagagta gcaatagcaa gggctcttgt aaataaacca tcaattgtat
     1141 ttgctgatga acctactgga aatttagaca caaagacaga gtcagatgta ttaagtttat
     1201 taaaagaatc tcaaaaaaaa tataatcaga caataataat gataacacat aatatagata
     1261 taacaaaata tgctgataga gttatagaaa tagaagatgg atttataaga gagtaggtag
     1321 atttatgaaa cttaattata ttgtaaaaaa atatataatg ggaaataaga aaaatacatt
     1381 attaataatt gtaagtattg taatttcaac agcgttgttt ttaattatga atataataag
     1441 tgaagatgcg acaaacttaa tgataaatca agctaaaaat gaatttacat taaaacatgc
     1501 acaatatatc aatccaaaag acgaagaaat aaaatatata gaaaataaca caagtataga
     1561 taaagttgga aaatcaatgc ttttaggaat tagtgatatt ggtaaaggtc aaacactaca
     1621 aattctatct caggataaag ttgctgaaga actaaatgat atttacacac tagaaaaagg
     1681 taaattacca gtagctgaaa atgaaatcgc tatagattca tggtatatta aacaaaaaaa
     1741 gataaaaaac cctataggaa aaaggataac attagattat agaagacaag ggatagatca
     1801 agaagattta tatactgggg aaaaagaatt taaaataact ggtatcttaa atagtaatcc
     1861 tattttaaaa gctcaaggaa catcaattgg ccttataagt aacgattgtg ctatcaagaa
     1921 tattcctact aaaaataaat atgatcaagt tatgtttact tttaaaaagg aaaaaaatat
     1981 acagagacaa tcacaaaaac ttattaaaaa tggaaatttg aatgagaata acatttattt
     2041 taataatgaa ttattaatag ctatatctga tagtatgaat ttaaaaatac cttatataat
     2101 agttaatata gtactagcac tagcaactat attattaatt tataatattt tttatatatt
     2161 agtatcaaat agaataaagg attttgggat atttagggct ttagggttta atccaaatga
     2221 tatttttaaa ataatgattt tagaagtatc tatatactct attataagta tttctatagg
     2281 tct
//
LOCUS       DAELTA010000277         2221 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.282, whole genome shotgun sequence.
ACCESSION   DAELTA010000277 DAELTA010000000
VERSION     DAELTA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.282"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..866
                     /locus_tag="KPG13_003556"
     CDS             132..866
                     /locus_tag="KPG13_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG0987237.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1009..1500)
                     /locus_tag="KPG13_003557"
     CDS             complement(1009..1500)
                     /locus_tag="KPG13_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG0987238.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1849..>2221
                     /locus_tag="KPG13_003558"
     CDS             1849..>2221
                     /locus_tag="KPG13_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987239.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQ"
BASE COUNT          862 a          246 c          325 g          788 t
ORIGIN      
        1 catattcatt tatattatta aaaaatttgt ttatagaaac ttttattatt ttatataaaa
       61 gtgataaaaa cttatatttt gtatttaata gagtgttgct aagtgaatat atttaaaatg
      121 gaggaaactt tatgaaacaa aataaatatg atgatgatag attttttaat aaatacagta
      181 aaatggaacg ttctacaaat ggattagcag gtgctggaga gtggcatgtt ttaaaaaaaa
      241 tgttacctga tttcaaagga aaaagagttt tagatttagg atgtggattt ggatggcatt
      301 gtcaatatgc agttgagaat ggagcaattt ctgctgttgg agttgatatt tcagaaaaaa
      361 tgttaaaaga agctagaaac aaaacgcaac ttgataatat aaattacata tgtatgccta
      421 ttgaagatat taattttcca aaagatagtt ttgatgtagt tattagttcc ttggcatttc
      481 attatattca atcatttgaa gatatatgta agaatataag taattgtctt tcaaataaag
      541 gtaattttgt attttctgtt gaacatccta tatttactgc atatggaagt caagagtggt
      601 actgtgataa agatggcact aatctacact ggccagtaga tggatatttt gaagaaggag
      661 tacgtaagtc aaattttcta ggagaagagg ttataaaata tcataaaaca ttaacaactt
      721 atttaaatac gttaatgaaa acaggatttg aaatattaga aattattgaa cctcaaccag
      781 aagaaaaact gttagataca attcctgata tgaaagatga gctacgtaga cctatgatgt
      841 tacttgtatc tgcaaaaaaa agataagtaa atataaataa tcattattgt agaaaaaaag
      901 ttgtagaaaa taattacact aatggagatg attataccag tggtattaat tacaccagta
      961 gtaaggaggc aaaagcattc gttgcttttg ccatttttta agttgcaatt atacttctac
     1021 gtagtatatt tcgtttccat tttcttctat aagctttttc atatcttcaa attttaaaaa
     1081 tacagtagca gtattatcat ttggatgtat acctatttta tccattttta ataaatcttt
     1141 atcaaataca acttttacaa cagcctcttt atcatttaat ataccaaaag gagtcacagc
     1201 tcctttagta agtttcaagt atttttgaag tctttcttcc gatgcaaaac taagaggttt
     1261 actatcaatt tgttgacgaa tatcttttaa atctgctttt ttgtctttac ctaatacaac
     1321 taaaaaatgt tcttttcctt tagcatcacg aagaaatagg ttttttacaa caaattccat
     1381 atgtaatgtt tttaattctt ccatttcttc aattgtataa actggtggat gatttacagc
     1441 ctcataaggt atgcttaact catctaattt tttatatact tcctgttgtt gattgttcat
     1501 gatttcctcc cattagaaat ttattttata tataaataat attatcacag aaaactttaa
     1561 aagaaaacgt tttatataat atagacaata gattatattt ttataaagaa gcaagaatac
     1621 tcaataaaac ttaaagaaat taaacaattt ctaaaaatac cttgaaaata gaaccttatt
     1681 tgccatttat aaattatata aaaagggatt atataaagtc atatatgaaa aaactatatt
     1741 atactatctt ttctttttgg aataaactat actttaaata tagatattta atttaaataa
     1801 attttacagt attaatagta aattatctta gaaaggaatg tgctttatat gtctacagac
     1861 aaagttgttg gaatcataga tgaagaaacg gcagaacttg caggaattga atatactggt
     1921 aaaatttatg cttcttctgg tgttataaaa catataaaga aaaaacatag atgtcaactg
     1981 agtaaggata tttttaatga tataatagac actataaaaa tggtattaaa gtcacctgaa
     2041 tatataggaa gtcatcctaa aaaacctgga aaaagtgtgg agtttataaa aaaaattgat
     2101 gattatattt tagtagctac agaattagat acaaaaaaag gatatttata tgtatcttca
     2161 atgtatgcta taaaagaagc aaaattagaa agtagagtaa acagtggaag agtaaaacaa
     2221 t
//
LOCUS       DAELTA010000278         2202 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.283, whole genome shotgun sequence.
ACCESSION   DAELTA010000278 DAELTA010000000
VERSION     DAELTA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.283"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            273..539
                     /gene="rpsT"
                     /locus_tag="KPG13_003559"
     CDS             273..539
                     /gene="rpsT"
                     /locus_tag="KPG13_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG0987240.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(726..1769)
                     /gene="holA"
                     /locus_tag="KPG13_003560"
     CDS             complement(726..1769)
                     /gene="holA"
                     /locus_tag="KPG13_003560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG0987241.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1723..>2202)
                     /locus_tag="KPG13_003561"
     CDS             complement(1723..>2202)
                     /locus_tag="KPG13_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG0987242.1"
                     /translation="LSFLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFK
                     GIPLLSIIGNLIIVPFVGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEK
                     ISNLSFAYINIENPKFYIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT          819 a          312 c          211 g          860 t
ORIGIN      
        1 aaaatgtatg ttaatagttt gtattatctc aaaatatttg ttacaaaaat aaaatattaa
       61 tttatatatg tcaaaatatt taaaacacaa aatataccaa tttaaaaaca agatattgca
      121 aattatttat acacaattta cagtctaaaa aatcaaaata aaaaacatta ttaaaatata
      181 taaataataa tccttgattt ttagatttat ttctgttaaa atctaatatg ttgacgatga
      241 atatgataaa aaaatacagg gaggtgaaca cggtggcaaa tataaaatca gctaagaaaa
      301 gaataagtgt tatcgaaaag aaaactgctt taaatagagt tagaaaatct caaataaaaa
      361 ctgcaataag aagattcgaa gatgctgttg cagctggaaa cagagaggat gctgttgcta
      421 agttccaata cgctcaaaaa agaatatacc aagtagcttc taaaggaact atacataaga
      481 atgctgcagc tagaaaagta gctaaattag ctcaaaaatt aaacggtatg aacgcttaat
      541 taccagttta tatttcttat atataataac ccctatgtag gggtttttta ttttgtaaat
      601 ttatacaata aaaaataaat agttagacat attatagttg attgtatatc aaacttaatt
      661 tagcttacat ctactctgaa ctataacata tcttgctatt tattttttat taatatatag
      721 attttttaaa ttgcttctct tttacagtat ctactaacca acatttcaac tgctaaagta
      781 tctcttataa gacctgtctt tattttataa tcactttcta atatatagtt taatatttcc
      841 acaattatat catcagaaaa atttttagtt tgctttaatg cttttcctac aacaaattga
      901 tgcattttta atttatcagc tataagtttt gttgaatatc catcaagttg taactgtctt
      961 acttgcataa ttattttaaa ttgtcttgct atcatagaaa atatacctaa tacagattcg
     1021 ccttcttgta tcatatcatt taatattttc attgcattag aagcattttg ttctccaata
     1081 taatcgatta atttgaatat atcattctca actttttttt gagataattt atcaattaca
     1141 tcattggtta cattattccc tttaccaaca aatgaactta ttttatttat ttcattttct
     1201 aaatcagata atgtcttttc actactctta tctctgtatc cttcttgttc aataaaatac
     1261 atgatttgag aattatctat tataacatca tttaaagcaa aagatttttt tatccattta
     1321 aacaaatcca tatcagaaag cttatcacaa tcaaaaacaa ttccattgtt tcctattttc
     1381 ttaactaatg attttctttt gtctacatct ccatatacaa caaaaactat agttgtggtg
     1441 tctggaatat tgtctaaatg ctctattaag tatttctcat cactatctgt aaaattcttt
     1501 ttctttcctt ttaatagttc aaagtctttt attattacta tctttctatc atccataaat
     1561 ggaagtgttt ctatagagct tataagctga tttaagacta tttccttccc atctattata
     1621 tctaagttaa aatcaagcat tccctcgttt agactatctt taaatgcttt tattgcattc
     1681 tcaattaaat aatactctct tccataaaaa agatacattt tttcaaagtt tttctctttt
     1741 acattcctta taatatcctt gtaattcatt ctcttgctcc tttatctctt ttccttctat
     1801 gtaaaatatg taaaaaaaca ctcctatgta ataaataaca acaatataaa atttaggatt
     1861 ttctatatta atgtatgcaa aacttaaatt gctgattttt tctagtaaaa aaaatatgct
     1921 ttctataaat gtactattaa aaaaagatat aattttagct attactacac ttactttaaa
     1981 tacgattaaa cttgcaatac ttaaatacat tattactccc acaaatggta ctataattaa
     2041 atttcctata attgaaagca atggtatacc tttaaatgta taataaatta taggtaaagt
     2101 taatatattt gatgatattg tcaatgatac catacttaat tttataatac tatttatttt
     2161 attatagaat attaatatag aaagtgtagc taaaaaagac aa
//
LOCUS       DAELTA010000279         2174 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.284, whole genome shotgun sequence.
ACCESSION   DAELTA010000279 DAELTA010000000
VERSION     DAELTA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.284"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            212..2116
                     /locus_tag="KPG13_003562"
     CDS             212..2116
                     /locus_tag="KPG13_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG0987243.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT          913 a          224 c          414 g          623 t
ORIGIN      
        1 acatgttaga ataagtctta tttgaaaatt ttagtgtact agagtaagta ttattttact
       61 agtaaaaaat attgagaact atatgtagat ttgcttaaaa aggaatttac atatagttct
      121 tttcgtataa taaatagtac aatcttatat ggtaaagata aagtgtgata ttaaatttta
      181 ttatcatcca aaatctaagg aggagtctct tatgaaaata aaatttatta tagtagcatc
      241 aataatattt ttaatagtat tacttgtggg gtgtcaaagt actaaagaca cagaaatcaa
      301 tgtactcgcc acaagtgatt tacattcaat tattccagat aatttaattt cctatgtaga
      361 agaggagaga aaatatgatg agaatctact tatggtagat gcaggagatt tttttgacat
      421 gcaaagtggt gagatgaata agtggtttac tggtcaaaaa ttagtaagat ttaaagatgg
      481 gataccagag tacaaaaaaa tagctgaacc acgtgaagga gaagttccat tggctaaaaa
      541 aatggcaaag ctgaagtatg atgcagttac atttgggaat catgaatttg tatctaatga
      601 caagcagagc cttgataaat tggtttctga ttttaaaaat aacaatatac cattagtatc
      661 agcaaacgta tatgaacaat ctggagaaaa ctacgtaaag ccatatgtaa tgaaaaatat
      721 aaatactgac aagggaactg taaaggttgg tatacttggt ctgactataa aagaagtagg
      781 ggaacgttca aggtttaaaa gttttgaaca agataaaaag gctagagaac ttggggaaca
      841 acctcaatac aaagggaaat tatatgcaaa tgatttagta gaagatgcaa ataagtgggt
      901 aaatgttatg gaaaaaaaag aaaagccaga tataatagtt gccattgtac attcaggtga
      961 agagccaaaa aatcctaaaa atccaggaaa tagaataaaa gaactagcaa caactgtaga
     1021 gggaatagat gctattgtag caggtcatac acatgaaaaa atagaacagc atacatataa
     1081 aaataattct ggtgaagaag taatagtaac acagcctgga gctcatggta atagtatatc
     1141 aaagattaat tttaagttgg ataagaagaa tgataagtgg tttataaaag acaaatatag
     1201 tgaattaaag gtttttggaa aggaagtaaa tataattaca acttctggtc tgaatccaaa
     1261 attttcagat gaaatggcag ttaatttgtt taatgaaaga gatagagatg ttcaacctat
     1321 ctttatagat actggagatt ttttaaatac taatacaaaa gaaatgacta agtgggctaa
     1381 agagtggcaa tacaatgaaa ataaaggcat ctataaaaaa ataaatgaat ggccaattgt
     1441 atatcttggt tatgatgctg tagtacttgg tagccatgaa tttacagagg ataaagagac
     1501 ttttgaggaa aaaaaatcta cattggatta tattatagaa aactttgaag aaatgaagat
     1561 accagtatta tcagctaata tatatgatga atctggagaa aattatgtaa aaccttatat
     1621 aattcataat gtagaaacta gggaaggaaa tataaaggtt ggagtattag gtcttactac
     1681 tactgataag gatgaagaag acttaagtaa ctcaagctta agcaaagact caaagacttc
     1741 gaaaattcct gaagaaacag gaaagttata tactaataat ctagtaaaag atgcaagcga
     1801 atgggtaaaa gttatgcaaa aggaaagtcc tgatgtgatt gttgcagttg tacattcaga
     1861 taataatagt actctaaaaa atccaagtag tgaagtcaaa aaacttgcaa tgtcagtaga
     1921 tggaatagat gctattgttg taggtcatac gaaaaataag gtagaagaac atatttacaa
     1981 aaataagtct ggggatgagg ttatagtaac acaatctgga gaagattgtt attcaaaaat
     2041 aagtcttgaa ctaagaaaag aaaatggcaa gtggaatatc ataaacaaat atagtaaagc
     2101 aataaaaatg aagtgaattt attgatatat aaaccattat taacactgat ttttataata
     2161 taataaatac tgat
//
LOCUS       DAELTA010000280         2147 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.285, whole genome shotgun sequence.
ACCESSION   DAELTA010000280 DAELTA010000000
VERSION     DAELTA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.285"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            121..519
                     /locus_tag="KPG13_003563"
     CDS             121..519
                     /locus_tag="KPG13_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG0987244.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            507..1445
                     /locus_tag="KPG13_003564"
     CDS             507..1445
                     /locus_tag="KPG13_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG0987245.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1829..2053)
                     /gene="secG"
                     /locus_tag="KPG13_003565"
     CDS             complement(1829..2053)
                     /gene="secG"
                     /locus_tag="KPG13_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG0987246.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          795 a          279 c          256 g          817 t
ORIGIN      
        1 ttatataaat attatcaatc tatataaaat ttaataattt atataaatat catcaatcta
       61 tataaaattt aataacttat ataaatttaa ctaatttata taaaatctta aggagttgcc
      121 atgataaaaa aaatagaaca tgtggtcatt actaataata atatagaaaa ttatatcaat
      181 ttttataaaa aatttggttt ttcatctaaa gaaatgagca aaaattataa acttaatgct
      241 agaaatgtta agataaatct taacataaaa ggttataaat ttttacctta tgttaaaaat
      301 tcaaaagcaa gtagagataa cttatgtttt aaaataaaca caaatattga tgagccaaaa
      361 aaatacttag aacaaaatga gattcaaatt aagagaggaa ttgtagaatg tacaggtgtc
      421 tttggtaata tgaaatctat ttatttgaaa aaaactaatg gaaatttaat tgaattatca
      481 aatcatagaa tcacggagag taaaccttgg aaaatttaat cctatcaata aatgttgttc
      541 ttcctttgtt tctcacaatg tcacttggat atgttctaaa aaaattaggt ctatttgata
      601 ctcatacttt aaataaaatg aataatgttt gcttcaagtc tttcttgcca ttattattat
      661 tttttaatat atacaataca gatttagatg gttctcttaa ttttaagctt atggctgtag
      721 ctgttataat tattttttca atcttcttaa tcttagtctt tattattcct aaaattgaaa
      781 aagataataa gacaaggggt gcattaatac aaggtgtatt tcgtagcaat ttcgttatat
      841 ttggtattcc tgtgacaatt tcactatttg gcgataagag tattggagtc acatctttat
      901 taattgcagt tattgtacct atgtttaatg tattatctgt tgtaattctt gaaattttta
      961 gaggtggcaa agtaaatata aaacatatga ttaaaagcgt cttaacaaat ccactcataa
     1021 tcgcctcttt tataggattg acaatgctaa ttttaaatat taatatacct acatttttac
     1081 acaaaactat tggtgatatt tctaaaatag ctactccttt atcacttata attctaggcg
     1141 gctcttttag tttttctgct ataaaggggc atataaaacc cattatattg ggcgtatgtg
     1201 gaaaacttat acttgtacct gctatatttt taccaattag catttttctt ggatttagaa
     1261 atgtagaatt agcttcactt atgataatgt tatctgctcc aactgcagtt tcttcattta
     1321 caatggcaca acagatggat tctgacagtg aattagctgg acaaattgta gtatttactt
     1381 ctggattttc tataattaca gtatttttaa taatatttat tttaaagcag aataatctta
     1441 tttaatgata tttgataaca caaattggta ttataagaaa catatataaa actgatttaa
     1501 tcgcgtaaaa acatattatt atactatttt tactatataa tataactttg tgtatattat
     1561 ctcattaaac atggtgaaag tagtgttgct atatattttt tagtttgaag aaaatatata
     1621 aatacaaaca atatataaat acaaataata aacgccacca aatagtcaat ctataaaata
     1681 aaaactggac taatcatata aaatcattag tccagctttt attttacgta ctttttatgt
     1741 tgatgtactt ttattaaaaa ctaaatttaa tttttaaatg tctagcaaat gcactaatta
     1801 aataatggcc agttattcaa caatatcact acttcttaac tataagtaat gccaatgcat
     1861 ttataaaaaa taatactgct gttatctttg taactctagc aagaaatgct tgttttcctt
     1921 ttggtttaaa gtaagcttga cttccttctc caccaaattc tgaaggtaaa gcattcttag
     1981 aatcttgagg cattatgcta attactagta aaatccctag tactacttgc acacccatta
     2041 aaatgttgct cattcttgcc cctcctatct gtttattact taaatttcat tactctttag
     2101 tgtatcacaa taatatagcc atttcaagac tattatgcta taaataa
//
LOCUS       DAELTA010000281         2104 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.286, whole genome shotgun sequence.
ACCESSION   DAELTA010000281 DAELTA010000000
VERSION     DAELTA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.286"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            266..709
                     /locus_tag="KPG13_003566"
     CDS             266..709
                     /locus_tag="KPG13_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0987247.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            881..1051
                     /locus_tag="KPG13_003567"
     CDS             881..1051
                     /locus_tag="KPG13_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987248.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            1424..>2104
                     /locus_tag="KPG13_003568"
     CDS             1424..>2104
                     /locus_tag="KPG13_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HBG0987249.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYS"
BASE COUNT          832 a          191 c          340 g          741 t
ORIGIN      
        1 tctaggtaat tgctagagct gatattataa tttattatta gtattgatat tctaggtaat
       61 tgctagagtt agtattatag atgaatatta gtattgatat tctaggtaat tgctagagct
      121 gatattataa tttattatta gtattaatat tctagggaat tatcactata ttttaaaaag
      181 taaaaatata tatcaaaatg aattaaataa gtactgtaag aagtttgtta aattaaggtt
      241 tgaataaagt gagggtggag aacttatgat agaaataaaa actatagaaa taaataaaaa
      301 aagtcgaatt gaccttttaa tgttagccga cccatgtgaa gaaatgattg ataaatatct
      361 caataaagga actatgtacg cattgtatga taataaagaa cttacttgta tagcagttgt
      421 aaatgaaatt tccaaagaaa tatgtgaatt aaaaaacatt gctacatatg agcattttca
      481 aaatatgggt tatgcctcaa agatgattta tcatctttta gatgtttata gtaaaaaata
      541 ttcatcaatg gttgttggta catctgaaag cggagttcct ttttatgaga agtttggatt
      601 tgtttattca cataaaatta aaaacttttt tgtagataat tatccagaac caatctttga
      661 aggagaatta caatgtgttg acatgttgta tctatcatat aacttttaga taggataata
      721 tttttaggtt aatataatta attaattcat ataattatcg gaattataat ttataaatta
      781 tgtataataa gagtataatt tatttagaag ctgttatatt gtattatatt caaataagat
      841 atataataac ttgtttattt tgaattttgg aggattaatt atggattcaa ataataatac
      901 tataaaatca actgttaaaa agggtatttc ttttggttct tgtttagcaa tgattatttc
      961 ttatactgca tggaaatcta ttccatgggc tatttttcat ggcttaatga gttggatata
     1021 tgtactttat tattgggtta agtatgcata gattaaatta ataaataagt atgtaatatt
     1081 gtaaagttta agaaacaaaa tgtaagggag tgtctcgaag tgaactttta gtttataagg
     1141 cactcttttt tgtattaaat aaaatatatt ttcattattt aaacaatgaa aaagatgagt
     1201 atctctattt taatacatac ccttatattc attcatttat caacaatagt tttaaaaaga
     1261 aagctatcta aaatctattg taatctaagt gcttctcttt aaagtcttaa ctttagtaat
     1321 gatatataag tttataaggt tcttatttca ataatgcaaa ttttacttag aagttcagac
     1381 tttggtatga agaaatgggt aataataaaa aagaggtgtt gatatgaaag aaaaattaga
     1441 agaaaaatct aagattatta aaacatcaaa agagtataaa cgaactataa ttgaagaaaa
     1501 ggacagagaa tggaccaaaa aattaaatga catacttagt agaggtgatt tttctaatga
     1561 aaaattagat aaaatcaagg atttaattcc aaaagaaata ttgactagtg aaaatgttgg
     1621 agaagttgta actgaagatg ggtatgctaa gcctgagtat gatgaggtat ttaaatcatt
     1681 gtttacttta aataatgatt acgatttact tgcaaatttt ataaatgata tcctcaaaga
     1741 tgctccatat gctaatagga atatcaaaca gttcactcaa ataaaaagaa taattaaggt
     1801 tgaaaccgac ccaacaatta attacattgg tgagaaaaga cctagacttg atattttggc
     1861 tgaagatgaa gaacgtaacc atataaatat tgagatgcaa agagctcttg aagaggatta
     1921 tttggaaaga gctgaatatt atcttagtcg tgtacatggt agaaagttag aagaaggtaa
     1981 agaatataaa gaaattggaa aaactgttgg aatacatata ctaaatcatg taaaatataa
     2041 tcacatagaa gattatgtaa attgcctgag attaacaatg gacgggcatc cagatattta
     2101 ttct
//
LOCUS       DAELTA010000282         2045 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.287, whole genome shotgun sequence.
ACCESSION   DAELTA010000282 DAELTA010000000
VERSION     DAELTA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.287"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..1046
                     /locus_tag="KPG13_003569"
     CDS             <1..1046
                     /locus_tag="KPG13_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0987250.1"
                     /translation="ILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSF
                     FRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPK
                     LVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDIN
                     NHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIK
                     FTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVL
                     KCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNI
                     YIPLDQDTDSWGD"
     gene            1287..1976
                     /locus_tag="KPG13_003570"
     CDS             1287..1976
                     /locus_tag="KPG13_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG0987251.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
BASE COUNT          852 a          185 c          312 g          696 t
ORIGIN      
        1 gaatactatt ttttgcaatt acaggtaatg ttatttcatc taaagaatct ttattagtac
       61 cttacttaga atctataaaa attcagatat atagtaattt aataattatt cttatatatc
      121 ttatatttag cttttttaga aagtctataa aattaattag ccaagataag agaaattatg
      181 tatatttatt atttgcctta ttagttaata caatgaatat gcttgtaaat tatgtattaa
      241 aaaaattaga acggtttggg tcattacatt cagaaggata ctattttgat aattttgtta
      301 atccaaaatt ggtaggagct agtagtatat ttcttatact attgtttaaa gaaataataa
      361 aagagaatag attaaaatct caagctgaat taataaaaaa taagttagat atgcaatatg
      421 cgcattattt aagtattcaa gaatctcata tgcgagttaa gaaactatat cacgatataa
      481 ataatcacat ttattgtata gacaacctta gaaacaatag caaagaaatt aatgaatatg
      541 ttaacaattt aaaagatgaa ataaaaacat ttaaatatat ctataataca ggaaatatga
      601 tattagacat tataatcaac gaaaagagtg aagtatgttt aaaaaaagga attaaattta
      661 cttgctctat aaatttttca aaagtaaatt ttgtaaaacc tatagatgtg tcaagcatat
      721 tttctaatat tttggataat gcaatagaag catgtgataa aatagtggat gaaaatatta
      781 ataaatatat tagaattaaa ggaactataa caaggtcatt ttttgtactt aaatgtgaaa
      841 atagtaaact aaatcagctt acttttaata aaaatatatt gttaacaaac aaaatggata
      901 agttcgtaca tggaataggt attcagagta ttaaatcttc tttacaaaaa tataatggag
      961 aattattatt tgaaaatagt atcgataaat ttatactaaa tatatatata ccattggacc
     1021 aagatactga cagttggggc gattaagtta ccagttggga catatgtatt gatacaaaaa
     1081 aataaggatg ttatctttta ataacaaaag ataacatcct tatttctgta ttaaggagct
     1141 tataatttta ctaatagtaa gttataagaa ttggatagga aggtgatatg tattgaataa
     1201 taaatcttta atataaagaa aatataaata tattctagtg caatgaataa tactttagag
     1261 cggttagtgt gatgggagga ttgtgtatga aagtaaaaag gaatataaat agagtggcaa
     1321 taattgtggc tttaaatctt agcttagcat tgatggtagg gtgtatgtct cctgtatctg
     1381 ataagaactt aagtagctca gaacaaatta gtaaagattc attgaaagaa caagttgaag
     1441 ataaaaaaga tgaatctatt gagctattta ataattactc acaaaagatt aatgatgcga
     1501 aaggtagaga agaagtaaaa aaagatttat ctcaaagatt ttcgaaagct ataatagaca
     1561 gtggttatag tattactaac tcaaaatcag atggtagctt tgtagtatct ataaatggtg
     1621 ctgaaaaacc acctgaaaaa aatattcgaa gtttattcta ttctatgtct gaagacgata
     1681 aagaaggaaa agtaacaata gatattctct gtgctaaaga gtatccagaa gatgataagt
     1741 tatctgagag tgataaatat gttaaattta tatacaattt atttaaatca ttgactgata
     1801 cgaacttaac tgagaaagaa attttttcta tggttgaaga agactttaat aagggagtag
     1861 gtgttgtaga gcttccttat atgaaggata tacatgttga agttaacaaa gttaaacaat
     1921 caactaaggt tttaaaacta agtctagtat acaaatttga tatttcaaat cattagtata
     1981 ttagacataa ctataacaat atagaaaata tatcatagaa aattttttta gattttataa
     2041 attta
//
LOCUS       DAELTA010000283         2020 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.288, whole genome shotgun sequence.
ACCESSION   DAELTA010000283 DAELTA010000000
VERSION     DAELTA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.288"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            275..1570
                     /locus_tag="KPG13_003571"
     CDS             275..1570
                     /locus_tag="KPG13_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG0987252.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT          834 a          168 c          385 g          633 t
ORIGIN      
        1 caggtatagg gaacgataaa tatagttcat ataaaatata gtgagtactt aataaacata
       61 tagtcaatat cagataaaaa aataatagct tatgagaaaa attaaaatac aaggtattag
      121 taaccttgtg ataagataaa gtgagtagtt taaaataaga atataaggaa ctatttataa
      181 aagaatataa tgaagctaat agagtataag aaaattactt tattttagta aaataatttc
      241 aaataaaaat ttttttaaat tgggagtata aattatgaca aatgatttta aaaaagaaaa
      301 gaaagaagat tattttgtta atcttaaggc aatatctact gaagttttac aagaaaaagt
      361 gcaagataac gcttgggtta tagttgatac tcgcttaaat gatgcttata atggctggaa
      421 gttagatgga gtaaaaagag gtggacatat aaaaggagca gtagattttt ctgctaattg
      481 gttaagtgta tactcagata gaaaagatga agtgttagaa caagcattga aaactaaaag
      541 aattgattta gataaaaata tagtattgta tgatgcaaat ggaaaagatg ctttggttgt
      601 agcagattat ttatctaaaa aaggatataa atatctatat aaatatgata ttaaacaatg
      661 ggcagacgac gaaaatcttc caatggaaag atataaaaat tatcaaatga tagttcctgc
      721 ttttataata aaagatatat tagatggaaa aattcctgag acatttgaag attcaaagaa
      781 tataaaaatg attgaagcga gttggagaga agaatcgtat acaaaagggc atatacctac
      841 aagtgttcat gtaaatactg atattataga acctcctcca acatggatgc ttgataatga
      901 tgataactta actaagtttg ctttagacta tggattaaca aaggatgata ctgtaatagt
      961 ttcaagtagt acacctatgg catcttatag actagctgtt atattgagat atataggagt
     1021 aaaagatgta agagttttga atggtggaac aaactcatgg ttatcagcag gatatgaatt
     1081 agaatttatt agtaatccaa aacattcatg tactaatttt ggagcagata taccagttaa
     1141 ctcacaatta attgttacta catctgaatt aagacaaaaa ctaaaagaaa aaaataagtt
     1201 tatactagta gataaccgta cttgggatga acatatagga aaagtatctg gatatacata
     1261 ttatgataaa aaagggcgta taccaggagc tttatatgga cattcaggta gtgatagtgt
     1321 ttcactagaa gaatatcgta atattgataa tacaatgaga aataaatatg agattttaga
     1381 aatgtgggac aaggaaaata tagatgtgaa taaacagctt atatttatgt gtggaagtgg
     1441 atggcgtgct gctgaagtat taacatatgc aaatgtcata ggagtggaaa atacctccct
     1501 ttatagtgat ggatggatgg gctggagtct tgataattca aatttaattg aagtgggaga
     1561 acacaaataa agtttttgaa agtgcaatag agttgtttaa aacaaaggtg tttttgattt
     1621 attaggaaga gtgtgtaaat tgtgtattgg gaagtgtact taggtatgtt catttggagc
     1681 tatggaaatt gataaaaaag tattatctgt ttattttgtt ggtaaaatat gaatagatta
     1741 tagattatag attatagatt atagatttaa gtatagacta agtaatttat attacagaag
     1801 agaatatata agaattaatg caagatgtat ttgatacata taagaaattg gcaagtccag
     1861 gagaaggata gagaaaattg agattgaaga gtttgaaaga gattattaga tgaaaaatac
     1921 aaaaatgtat aaaaatacat attgagaaag tatatctctc ttaaatttgt taataattct
     1981 attatacaga aaaacatatt taagggggat tttttactat
//
LOCUS       DAELTA010000284         1965 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.289, whole genome shotgun sequence.
ACCESSION   DAELTA010000284 DAELTA010000000
VERSION     DAELTA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.289"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            320..613
                     /locus_tag="KPG13_003572"
     CDS             320..613
                     /locus_tag="KPG13_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG0987253.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            594..1235
                     /locus_tag="KPG13_003573"
     CDS             594..1235
                     /locus_tag="KPG13_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG0987254.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            1479..1811
                     /locus_tag="KPG13_003574"
     CDS             1479..1811
                     /locus_tag="KPG13_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG0987255.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          763 a          178 c          304 g          720 t
ORIGIN      
        1 aataggactt aactactttt taaattgggt ttagctactt ttaatttaaa attaaaagta
       61 ttgttatata tataagtacc tcaatcatta ggtttttgct ttaacaatta tggtgcaata
      121 cacttttgtt aagttaaaaa aattagataa agtaagatta aataaaagcg acttaagtaa
      181 attgagaatc ttaatataaa aattttcaat aaatatttag aaataactat ttagaaaaat
      241 ttctaaatag ctattgaaat aaaatggatg ttatgttata ttctatttag atgaaattct
      301 aaatagcgag gtgatggtgt tggggtttcc agaaacattc aaagctcttt cagaccctat
      361 aagaagagaa atattggtta tgttgaggga gaataaaatg tcagcaggag aaattagtga
      421 aaaatttaat atgacgaatg ctacaatttc ttatcatcta tctcaactaa aaaaggcagg
      481 actcatattt gaaacaaagt ataagaactt tattttctac gaaataaata tttcagtttt
      541 cgaagaggtc atgctttggt tttcacaatt tatggagaag ggagaaaaag ataatgaaaa
      601 aagcgataaa taaacaattt attttatcta ctttaatatg ttttatacca tttattgtat
      661 caatatattt ttataataga ttaccaaatg aagttgcaat tcattttgat aattatggaa
      721 atccagataa ttatgctcca aaggttatag cagcttttgg tgtaccattg ttaatgctat
      781 gtatccattt gtatacatgg tttagacttg agaatgagcc taagaaagct aatttttcaa
      841 atgttataca aaatttatca aaatggggag ttgctatact ttcagtaatt atacaaataa
      901 tgatggtttt atattcaaca ggagttagtt tggagtctaa tttttataca ggattaattt
      961 taggaataat agttatttta tttggaaact atcttcctaa atgtaaacga aattactcag
     1021 taggaataat tttaccatgg actcttaatg atgaagataa ttggaataag acacaccatt
     1081 tagctggatg gatttggctt attggtggaa tattattaat tattaatgca tttattaata
     1141 ttccttttta taatatcttt gtaatctttg taattgtaat actaccattt atatattctt
     1201 atttgatgta taaaatttca ggtaaaaata actaatataa ttaatcacta taataatgtt
     1261 tggataagaa taatatattt cagaactgag atagataata aagaaattac taagatttct
     1321 attttagttc tgaaaaaatc ttacttatat gataacttat tgagatttca aaaaataata
     1381 taaattaggc taatgaacat aaaatgaaaa tttgtattta atattgaaaa tataagtacg
     1441 gtgtgatatt cttataagaa aaacatagga ggtagaagat gaatactaga gaaatgaaag
     1501 aatatagggg attttacaat aaaaaattaa gcgaaacaga tgagtttttt agagtatttg
     1561 gaagtcttga tgataagact tattcagaag gggctataag caaaaaaaat aaggaattaa
     1621 tgggactagc tatttcaatt ttaagccgat gtgatgagtg tatttgttat catattgagg
     1681 gttgtattaa ttctggagca agtaaagatg aaattataga agctataaaa ataggtgtaa
     1741 ttggtggcgg gtctattaca tatccaaacg caagatttgc aataaaaatt ttagaagaat
     1801 ttaatatata attttgatag acgtagttta tttagactaa taataatagt ctatttaagt
     1861 ctttgaaatt aagctttatt ttctaagttc acttcttatt aagtttttag aattgcatat
     1921 ataaatataa atttttaaat aatatagtag ttttaaatta tttac
//
LOCUS       DAELTA010000285         1929 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.290, whole genome shotgun sequence.
ACCESSION   DAELTA010000285 DAELTA010000000
VERSION     DAELTA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.290"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            95..364
                     /locus_tag="KPG13_003575"
     CDS             95..364
                     /locus_tag="KPG13_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG0987256.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            390..1745
                     /locus_tag="KPG13_003576"
     CDS             390..1745
                     /locus_tag="KPG13_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG0987257.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT          720 a          205 c          418 g          586 t
ORIGIN      
        1 agtggtttac taacaaataa atgaaatata tgttaaaaca tagttcaatt tgtaaaaata
       61 taagatttat aaaaaaataa gggaggatat tattatgaaa gcaattttaa cagtaattgg
      121 aaaggataaa ccaggtatag tggcaggaat tagtgatgaa ttatacaagc aaaatatcaa
      181 catagtagat atatcacaaa aaatattaca ggattatttt actatgatta tgatagttga
      241 tttagagaaa tcattaaatt cttttgagaa aacggttgag gacttggttg aaagaggtaa
      301 gaaattatca gtggatgtaa aaattcagcg agaagaaata tttaattcaa tgcataatct
      361 gtagtagaca gtaaggggag gtgataatag tgaatgtagg aaatatactt gaaacaataa
      421 agatgataga tgaagaacag ttggatataa gaactataac tatggggatt tctttattag
      481 attgtataga ccctgatgga gataaagcta gagaaaaaat atacaataag attatgagtt
      541 ctgctaaaga tttggtgaag gttggaaaag atatagagcg tgaatttggt atcccaattg
      601 taaataaaag ggtatcagta actccaattt ccattattgc aggtgcaaca gatgaaaatg
      661 actatgtaaa gtttgcacag acattggata aggctgcaga ggatttaggt atagatttta
      721 ttggtggatt ctcagcatta gttcaaaagg gatatacaaa aggtgataag atactaatta
      781 agtcaattcc aaaagctcta gcaagtacta ataaagtatg tgcttcagta aatgtaggtt
      841 ctacaagatg tggtataaat atggatgcag tgaaagaaat gggagaaatc ataaaagaaa
      901 ctgcagaatt aacaaaagat gcaaaaggtt ttggttgtgc aaaattagta gtattttgta
      961 atgcagtaga agataatcca tttatggcag gagctttcca tggtgttggt gaggctgata
     1021 gaattataaa tgttggtgta agtggccctg gtgttgttaa gagagcttta gagaaagtaa
     1081 agggagagcc ttttgatgtt gtttcagaaa cagttaaaaa gactgcattt aaaattacaa
     1141 gagttggaca acttgtggca aaggaagctt ctagtagatt ggatgttcca tttgggataa
     1201 tagacttatc actagctcct acacctgcta ttggtgattc agtagcaaat atattagagg
     1261 aaatgggtct tgaagttgtt ggtactcatg ggactacagc agcattagca cttttaaatg
     1321 atgctgttaa aaaaggtgga gtaatggcat gttcacatgt tggaggactt agtggagcgt
     1381 ttataccagt atctgaagat gcaggtatga ttgatgctgt tattaaaggt gctttaagta
     1441 tagataagct tgaagcaatg actgctattt gttctgtagg tcttgatatg atagctgtgc
     1501 caggaaatac tacagcaggt acgcttggag ccatgatagc tgatgaggca gcaataggta
     1561 tgattaataa taagactaca gccgttagaa ttataccagc cccaggatgt gatgtaggtg
     1621 atatggtaga atttggagga cttttaggac gagcaccagt aatgcctata aatacaaatt
     1681 catctgaatt atttactcaa agaggaggaa ggattccagc tcctatacat tcatttaaaa
     1741 attagttata ttacatataa taatatttaa ttaaatattt agatggttaa attaaagaag
     1801 cctatgcatt taaatattat ataagataat gaactttgtt aagataataa actttaaaaa
     1861 tcagttatta agattatggc aaaaataaag cgatacatgg acatttaaaa attcatgtat
     1921 cgcttttat
//
LOCUS       DAELTA010000286         1926 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.291, whole genome shotgun sequence.
ACCESSION   DAELTA010000286 DAELTA010000000
VERSION     DAELTA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.291"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..236
                     /gene="rrf"
                     /locus_tag="KPG13_003577"
     rRNA            120..236
                     /gene="rrf"
                     /locus_tag="KPG13_003577"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            243..317
                     /locus_tag="KPG13_003578"
     tRNA            243..317
                     /locus_tag="KPG13_003578"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:275..277,aa:Asn,seq:gtt)
     gene            322..396
                     /locus_tag="KPG13_003579"
     tRNA            322..396
                     /locus_tag="KPG13_003579"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:355..357,aa:Glu,seq:ttc)
     gene            402..477
                     /locus_tag="KPG13_003580"
     tRNA            402..477
                     /locus_tag="KPG13_003580"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:435..437,aa:Val,seq:tac)
     gene            483..559
                     /locus_tag="KPG13_003581"
     tRNA            483..559
                     /locus_tag="KPG13_003581"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:517..519,aa:Asp,seq:gtc)
     gene            570..644
                     /locus_tag="KPG13_003582"
     tRNA            570..644
                     /locus_tag="KPG13_003582"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:602..604,aa:Thr,seq:tgt)
     gene            659..743
                     /locus_tag="KPG13_003583"
     tRNA            659..743
                     /locus_tag="KPG13_003583"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:693..695,aa:Tyr,seq:gta)
     gene            753..826
                     /locus_tag="KPG13_003584"
     tRNA            753..826
                     /locus_tag="KPG13_003584"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Gly,seq:tcc)
     gene            837..913
                     /locus_tag="KPG13_003585"
     tRNA            837..913
                     /locus_tag="KPG13_003585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:871..873,aa:Arg,seq:tct)
     gene            923..998
                     /locus_tag="KPG13_003586"
     tRNA            923..998
                     /locus_tag="KPG13_003586"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:956..958,aa:Gln,seq:ttg)
     gene            1010..1085
                     /locus_tag="KPG13_003587"
     tRNA            1010..1085
                     /locus_tag="KPG13_003587"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Lys,seq:ttt)
     gene            1088..1176
                     /locus_tag="KPG13_003588"
     tRNA            1088..1176
                     /locus_tag="KPG13_003588"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1122..1124,aa:Ser,seq:tga)
     gene            1194..1284
                     /locus_tag="KPG13_003589"
     tRNA            1194..1284
                     /locus_tag="KPG13_003589"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1228..1230,aa:Ser,seq:gct)
     gene            1293..1369
                     /locus_tag="KPG13_003590"
     tRNA            1293..1369
                     /locus_tag="KPG13_003590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1327..1329,aa:Pro,seq:tgg)
     gene            1455..1530
                     /locus_tag="KPG13_003591"
     tRNA            1455..1530
                     /locus_tag="KPG13_003591"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1488..1490,aa:Trp,seq:cca)
     gene            1591..1667
                     /locus_tag="KPG13_003592"
     tRNA            1591..1667
                     /locus_tag="KPG13_003592"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1625..1627,aa:Pro,seq:tgg)
     gene            1674..1750
                     /locus_tag="KPG13_003593"
     tRNA            1674..1750
                     /locus_tag="KPG13_003593"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1708..1710,aa:Ile,seq:gat)
     gene            1754..1829
                     /locus_tag="KPG13_003594"
     tRNA            1754..1829
                     /locus_tag="KPG13_003594"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Phe,seq:gaa)
     gene            1837..1913
                     /locus_tag="KPG13_003595"
     tRNA            1837..1913
                     /locus_tag="KPG13_003595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1871..1873,aa:Met,seq:cat)
BASE COUNT          485 a          443 c          523 g          475 t
ORIGIN      
        1 cattcattaa gatatatgtg cagtttttag agtgttaact ctaaaaaaaa ataaaaaaag
       61 tattgacaaa aatagtaaaa aatgttaata ttatacttgt cctagcaaca agaaaaaaat
      121 gtggttataa tagcggagaa gatacacctg tttccattcc gaacacagaa gttaagttct
      181 ctagcgctga tggtacttgg tgggaaactg cctgggagag taggacatag ccacgtgaat
      241 gttccaaggt agctcaacgg tggagcaacc ggctgttaac cggtaggctg tgggttcgag
      301 ccccaccctt ggagccatta tggctcgttg gtcaagaggt taagacaccg ccctttcacg
      361 gcggtaacag gggttcaatt cccctacgag tcaccatttg tgggatcata gctcagctgg
      421 gagagcacct gccttacaag cagggggtca caggttcgag ccctgttggt cccaccattt
      481 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
      541 agccccttcc aggtcgccaa aataaaaatg ctggtgtggc tcaacggtag agcagctgac
      601 ttgtaatcag caggttgtag gttcgattcc tatcaccagc tccatgtaaa actaatacgg
      661 aggatttccc gagcggccaa agggggcaga ctgtaaatct gttgtcatcg acttcggtgg
      721 ttcgaatcca ccatcctcca ccaattaaat atgcgggtgt agctcaatgg tagagttctg
      781 gccttccaag ccagctgtga gggttcgatc cccttcaccc gctccaaaga aagcgtgggt
      841 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      901 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      961 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa taaaaatatg gtctattagc
     1021 tcagtcggta gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat
     1081 caccaatgga gaggtgtccg agtggtttaa ggagctggtc ttgaaaacca gtgacttcga
     1141 aaggggccgt gggttcgaat cccaccctct ccgccaaaaa aatgactgaa tttggagaag
     1201 tactcaagtg gctcaagagg atcccctgct aagggattag gctgggtaac cggtgcgagg
     1261 gttcgaatcc ctccttctcc gccattcaaa aacgaggtgt agcgcagttt ggtagcgcac
     1321 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa cttaagcttt
     1381 tagttcagtt ggttagagtg cccaactcat ggctagtaat tctagggttt gagtccatga
     1441 aggttattct tcataggggt gtagctcagt tggtagagcg tcggtctcca aaaccgtgcg
     1501 ccgggggttc gagtccctcc acccctgcca aataaaaagg acttgaaaaa agttggattt
     1561 tagagtagta taagatagta aataagattt cgaggtgtag cgcagtttgg tagcgcacat
     1621 ggtttgggac catggagccg ggggttcgag tcccttcacc tcgaccaaga aaagggccta
     1681 tagctcaggt ggttagagcg cacgcctgat aagcgtgagg tcgctggttc gagtccagct
     1741 aggcccacca tttgcccaga tagctcagtc ggtagagcag gggactgaaa atccccgtgt
     1801 cggtggttcg attccgcctc tgggcaccag gaatacggcg gtatagctta gttggctaga
     1861 gcgttcggtt catacccgaa aggtcatagg ttcgactcct attaccgcta ccatctaact
     1921 atatca
//
LOCUS       DAELTA010000287         1918 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.292, whole genome shotgun sequence.
ACCESSION   DAELTA010000287 DAELTA010000000
VERSION     DAELTA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.292"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(350..1642)
                     /gene="eno"
                     /locus_tag="KPG13_003596"
     CDS             complement(350..1642)
                     /gene="eno"
                     /locus_tag="KPG13_003596"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG0987258.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          606 a          368 c          197 g          747 t
ORIGIN      
        1 aaaataaaac aatcatttta aaatattgat ataactttta atttcaacat aatttaaaat
       61 aaataatcca catttagcaa taatacttca atgacaatta tgattaaatc aaaatatgtc
      121 ttcactttta ttttttaata aaaacctcct atgcaattag ataaaaatga gatagaaaca
      181 tttattcata ttttttctga atctatgaat aaaattaagt aataaaatat aaaaaaggat
      241 aagtgaattg aaaaatcaat taacacttat ctcttttata tcttataaga tgagcttaaa
      301 tatcttatat tctctcttca ttaatctttt ttagtaacca ttttaataac tattttttta
      361 agttatagaa agattttaat ccacaatatc tagcttgctc tccaaccatt tcttctattc
      421 ttaataattg gttgtactta gcaactctat cagttcttga tggagcacca gtttttattt
      481 gaccagcatt tactgcaaca gctaaatctg ctatagtaga atcttcagtt tctcctgatc
      541 tgtgagatat aacagcagta tatcctgctc tcttagccat ttctatagca tctaaagttt
      601 cagttattgt acctatttga ttaactttaa ctaatataga gttagctact ccattttcaa
      661 ttcctttttc taatctctct gtgttagtaa caaataagtc gtcaccaact aattgtaatt
      721 tatttcctaa tttttcagtt aataatttcc atccatccca atcttcttcg tctaaaccat
      781 cttctatagt tattattggg aagttgtttg accaatcttc atataaagca accatttcag
      841 cagcagttaa ctcttttcct tctcctgcta aaacgtattt tttagtttct ttgttgtaca
      901 tttctgtagc agcaacgtca agacctaaca taacgtcttc acctggctta tatccagctt
      961 ttgttatagc ttcaactatt aattctaaag cttctctgtt tgaacctaag tttggagcaa
     1021 atccaccttc gtcacctaca ccacaagcta atcctttttc tcctaaaact ttctttaacg
     1081 aatggaatac ttctgctccc attcttaaac cttctttgaa acaacaagct cctactggta
     1141 atatcataaa ttcttgaaca tcaacgttgt tatcagcatg ctctccaccg tttaatatgt
     1201 tcatcattgg aacaggtaat tgtttagcat ttactccacc taggtattgg aataatggta
     1261 aacctatttc atcagcagct gctctagcta ctgccattga aacacctaat atagcattag
     1321 ctcctaattt acctttgttt ggagttccat ctaattctat catgattgca tctatagctg
     1381 gttggtcaaa tgcatccatt ccttctattt caggtgctat tatttcattt acgttagcaa
     1441 cagctgtttc tacacctttt cctaagtatc ttcccttatc tccatctctt aattcaacag
     1501 cttcaaaagc tcctgtagat gctcctgatg gaactattgc tcttcccatt gctccatctt
     1561 ctaatactac ttcaacttct acagttggat ttcctcttga gtctaatact tctctagcat
     1621 atactaattc aataactgac atttgtatat ctcccttcga ttttctatta gttaaatttt
     1681 catttactgt aaacatccgt ctactatttt aatctacttt ctatattata gtacataaag
     1741 ttgcttttga aaattaggta caaatttatt tgatgggtaa atttgtaaaa gttcctattt
     1801 ttattttatt ttttataata ttttaattta tataatataa aaaaacttgt ctaaaatcca
     1861 aaaccaatct taaatacaca aatattataa ctagatttaa aacattaaac ataaaatg
//
LOCUS       DAELTA010000288         1900 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.293, whole genome shotgun sequence.
ACCESSION   DAELTA010000288 DAELTA010000000
VERSION     DAELTA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.293"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..245)
                     /locus_tag="KPG13_003597"
     CDS             complement(<1..245)
                     /locus_tag="KPG13_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987259.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLER"
     gene            558..1367
                     /locus_tag="KPG13_003598"
     CDS             558..1367
                     /locus_tag="KPG13_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG0987260.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            1409..1675
                     /locus_tag="KPG13_003599"
     CDS             1409..1675
                     /locus_tag="KPG13_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0987261.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          718 a          230 c          229 g          723 t
ORIGIN      
        1 tttctctcca agtgaactac ttctccagtt aaatcatagt caaatgcctt aactaatatt
       61 tcagtaactt ttttaccatc tactacatca tcttttccat taaataaatc caaagcatta
      121 ttctcaatca ttctctttgc aaaatttaca ggtaacttta tatgtatttt atcgcctgta
      181 tgtgataaga ctcttattct taaaaacttt tctaaattgt ttcttggtat ttgttttgat
      241 gccatgctat taaatcactc tcctttattt tttatttaat aataaatttt attcttaaaa
      301 atatctttat atgtatttat tatgaaagta acactattat tttctaatct tgttttatat
      361 tccttcttct ttaatatatt tatttaataa atatctatga tttataataa ttgtatattt
      421 tgagatttta ctttacaatt attgtatcta aatttaacaa aatatcttat tagaatattt
      481 caattaaaac aaaactggtt tattatataa aaaataaatt agtatactta ttttttatat
      541 atcaggagga tatacttatg aataaaataa tcaataacat tttaaatacc tgcaaacagt
      601 atacatatat tttgccacat aaattgttaa gacagtatat agcacactat acaatatcta
      661 ttccagatac aagtattaaa gaaaatctta cattaatacc tgatgctagt ggatgtatga
      721 tatttaaatt tgacaaaaaa ggtatagaaa gcgcattctg gggagccaca acaaaaacta
      781 ctattgtaaa aaatgatata gaaaatgtac tttttagagt attcgttgag tttcgcccag
      841 gtggagtata ttatttgaca ggtctttctc aaagagaatc tacagattta aaaatttcac
      901 ttgaagattt taacacatta tttagcttag aggttaattc catttttgaa agaacaagta
      961 ctataaagga attagtagaa caattagata tgttattctt atcatactta ttgaaatcaa
     1021 atatagtaga tatgactata ccaattcttg aaaatgctag aaaacaaaac tctattatgt
     1081 ctgttaaaaa catttcacaa attagttgtt atagtgaacg acatttaaat agaattttta
     1141 ataattctct tggtatgagt gttaaatcat accttagatt acttcgtata aaccttgtat
     1201 tacaagaaat acaaaataat aaaataccat ttgcaacttt agcacaagat attggttatt
     1261 acgaccaatc tcattttata aatgatttta aatctatttg tggtgttaat ccaacaacct
     1321 atataaaaaa tttgtccgat ttttacaatg aaaagtataa attttgatat acaatcatat
     1381 aaaatttata tttaaggagt gacttaaaat gatagaatca agatgtggaa ttttatgtta
     1441 tgaatgtgga tacaaggaaa aagtcaattg taaaggttgt acagagattg agaaaccatt
     1501 ttggggtgaa caatgtcctg taaaatcttg ttgtgaagat aaaaacctta cacattgtgg
     1561 tctatgtgat acatttccat gtgaaatgct taatcaattt gcatatgaca aagaacaagg
     1621 tgatgatggc aaaagaattt ctcagtgcaa aaaatgggct gtactagcct tataatatac
     1681 tctcttgtta taaaataagt ttttgtaagg taaatttttt aaactgttgt agaaatttct
     1741 gcaacagttt ttataattaa atttaaaggt tatttaatta aattaataga atagtaattt
     1801 attgataaaa tctgattact aaaattggaa atataaaatc tcaatctctg tctatttaaa
     1861 atatatgtta caaaattgaa ttaaaataga cttaaatttt
//
LOCUS       DAELTA010000289         1888 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.294, whole genome shotgun sequence.
ACCESSION   DAELTA010000289 DAELTA010000000
VERSION     DAELTA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.294"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            104..1681
                     /locus_tag="KPG13_003600"
     CDS             104..1681
                     /locus_tag="KPG13_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG0987262.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT          782 a          182 c          362 g          562 t
ORIGIN      
        1 tattattgat gaaaaatgta ataattttaa actgtaaaat agtgtaaagt tattttataa
       61 tataaatata gtaagatttt aaacaaaata agggggaata ataatgaaag caccaaaaac
      121 tattttaaca atactgacaa tagcgcttac tttaagtagc atttctataa taccatcata
      181 tgcacttaca gaggaaaaat taataggtaa tggtagatat gaaactgctg taaagataag
      241 tcaaaaggca tacagttcta gtcctaatgt agtgctagtt aatgacaatt cattagctga
      301 tgcactttca gctacaccat ttgcaaaggc taaaggagca ccaatacttt tgacagaaag
      361 tgataagcta gatgatagga ctgaaaaaga gataaaacgt ctaggagcta aagatattta
      421 tttgatagga ggtactgctg tacttaataa agatatagag aacaagttaa aaagtaatgg
      481 acttaatgta gaaagaatta atggtaagga tagatatgaa acttcactga ttttggctaa
      541 taagcttaag gatataaaag atattaagga agttgctgta gtaaatggtg aaaaaggatt
      601 gagtgatgct gttagcgttg gggcaccagc agctcaaaat aagatgccta taattttgtc
      661 tagtcctaag gacggagtag aagcatttga taaatttata agggatgaga aagttataaa
      721 agcatatgta attggaggta caaattctgt ttcaagagct gtagaaaaga gtcttcctaa
      781 tgcagaaaga gtgagtggga aagatagaaa tgagactaat gctaaggtca tagaaaagtt
      841 ttacactgat acaaatttaa gtaatttata tgtaactaag gatggtagta aaaatgagaa
      901 tcaactaata gactcgttag ctgttggagt attggctgca aaaaatgagt ctcctgttgt
      961 tttggttggt aataaattaa acacaaaaca aagggacata cttagtacta aaaaattaaa
     1021 tactataaca caggtaggtg gaaatggaaa tgaagaagct tttgatgaaa ttaaaagtct
     1081 acaagaaaaa actgtgtttg aagcaaaaac tgttgaggaa ttaactgata tgataaatat
     1141 agcaagtcct aatgatatta taaactttaa accaaaagag aatactgtaa atgaagcttt
     1201 tagaatggta acaaataaac caataactgt gaatataaag ggtgattgtt caaagactct
     1261 tacagtagat atgcctaatg gtgaagtgaa taactatgct actttggtaa atgttatagt
     1321 tagaaatatt ggagaaggtg gatttaataa ccatgataca atcactatat tgtcagttcg
     1381 tgacaaaaat ggtagagtta tagaaaatac tagaaattca gatatagata cgttgatgat
     1441 attagcttct gctaatgata caaaattgat taatgatgga tatataggaa aacttataga
     1501 taattcatct aatagtgata taactaataa tggtactata gataaaaaag ttaatcaggt
     1561 tgaagattta gaggctaaag tagattcaat tgaaaaagct atagattcta taagtcaaaa
     1621 agtcaataaa atacaagata tactagataa gcttggattt ctaaagaaat ttcttagcta
     1681 agaatatata tatttaagtg aagtgtttgt tgtaattgtt ctaaagttat gtagttaaac
     1741 aggtttatat acaaatagat ttatagataa atatgtagtt tagtatatat ttgaataagc
     1801 tttggggagg taacatgggg attggaattg aatgtaacaa aaaaataaat aaaagtattg
     1861 acaaaactag aataaagaaa aataatct
//
LOCUS       DAELTA010000290         1839 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.295, whole genome shotgun sequence.
ACCESSION   DAELTA010000290 DAELTA010000000
VERSION     DAELTA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.295"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..563)
                     /locus_tag="KPG13_003601"
     CDS             complement(<1..563)
                     /locus_tag="KPG13_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG0987263.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRC"
     gene            complement(1654..1824)
                     /locus_tag="KPG13_003602"
     CDS             complement(1654..1824)
                     /locus_tag="KPG13_003602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987264.1"
                     /translation="MPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTTVSSLL
                     GRIAVNIFNFNK"
BASE COUNT          703 a          247 c          162 g          727 t
ORIGIN      
        1 tgacatctaa aaaaattata tttttctagc tctaattcta ttttatccat actattttta
       61 gtataataag tatcattttt agtatgtata gtcaaaacct ttttcctaac ctctatatac
      121 ataatactat taattaaaat tttattgatt atacctttat tttctatcag cataaaattt
      181 tctctttttt ttataatatc cgatatacaa ctcaaaatac tttcatttaa atctccaaaa
      241 tctataggct ttaataaata tctataagcc cttaccttat agccttcctg tatgtaatca
      301 actattgaag ttataaatat tatttcagaa gtatcattag tttctctaat tattttagcc
      361 acatccatgc ctgttagttg aagcatttta atatctaaaa aaaatatatc aataccttct
      421 aaatttgttt ccaataattc ttctcctgaa ttaaactcaa atagttcaat ttgactagaa
      481 ataccctcta aggactttaa tatacactct cttaaaaaag ctatttgtgt aatatcatcc
      541 tcacagataa ctattctata cataaacacc cctccttaga cattagatct gaaaacaaat
      601 aaaatatcaa tttataaata attatttagt tatacaacta acatggtcat caaatactat
      661 tattcactta aaaaatcacc ccctattttt agtatgttaa caacaaaata agaagatatc
      721 ttagattttt aaaaacaatt aatacttaat gtagttttgt atactattta taggtaattt
      781 tttatatcat tttaaacatt aaaattattg atgtttaaga actattatat tgatagtaat
      841 atataattaa tgatatcaaa aatttttaat tagacttctc tcttattcaa gatttttcta
      901 tataaaatct aagaataaat agaaaactaa catctaacct tttatttttt attttcgctc
      961 tatttacttg aattttaaca taaatctatc ttttagtata tattaatcat gctgaatttg
     1021 tgatgacatg tatacaaaat atcgtttagt attttctaat aaaaaatatt actgaaatta
     1081 aaaatctaca atagcattta acaagtaaaa acatgtttac acaaatatat aatttaaggt
     1141 ctttaaaata gagtatgtat tattctaaaa attagttata gttatcacat aaaaatagaa
     1201 aagaattttc aaaagattta aagaacatat atacttcaat tgcagaagat gtaatgttaa
     1261 gtgaactagc attcttcgag gaaaaataga ctcaaaaaat tagaaaatag aatcaggttt
     1321 tagatgattt aagtatttat tttcaaaaaa gaaatttgat tctaagttaa taaagaaata
     1381 tttaaaaaaa tcaaaaaatt ttcttaaata agtaataggt tttgtaaaaa tggaaaaata
     1441 tcaaaataag actatgattt ttaaaactat agccttatca ataaattttt atttaaagaa
     1501 cacacataaa tatttttaaa cctaatttat gtgaagtttt atgtattttt tatataagta
     1561 gtattctaat cttactcatc taatatacca aaaagactgt aagtaagttc acttttatgc
     1621 aaacttaccc acaatctctt ttacacatta attctattta ttaaaattaa aaatatttac
     1681 tgctattcta cccaataaag atgatactgt tgttgttctg gctggtactg ttacttctat
     1741 ttctcctgta tctgctgttt taccacttga tgtgtgtgtc actgtaaata ctaatgttac
     1801 ttttgtttct gtatctggtg gcactattgt tccatcttt
//
LOCUS       DAELTA010000291         1822 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.296, whole genome shotgun sequence.
ACCESSION   DAELTA010000291 DAELTA010000000
VERSION     DAELTA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.296"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(91..657)
                     /locus_tag="KPG13_003603"
     CDS             complement(91..657)
                     /locus_tag="KPG13_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG0987265.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            818..1471
                     /locus_tag="KPG13_003604"
     CDS             818..1471
                     /locus_tag="KPG13_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG0987266.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(1629..1805)
                     /locus_tag="KPG13_003605"
     CDS             complement(1629..1805)
                     /locus_tag="KPG13_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987267.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT          617 a          223 c          226 g          756 t
ORIGIN      
        1 atttagactg aaaattatat tttatacaat aaaagtttat ttaaataaca ttgtatttta
       61 atatttattt tcagtcttac tttattaaaa ttattttttg gttatgtctg gaaaatatct
      121 gtctactaat ttttctgaaa tattttttaa ttggtcagcc attgaagtca gacttaataa
      181 ggctatcaat aaatcatctt tattttctgg atttatacta atattttcaa aattagactc
      241 tataaagtcc ggtatattct ttctttgatt ttcttttatt aacatgaatt tttcgtaaca
      301 tgaaactaaa tctttttcac tgaaattatc cttttcatac acaccatgta agattctttt
      361 aatttcttgt attgtaagtg tatttttcat agaataaata attatcatct gtaaaatttg
      421 ttctttagta tacttttttc ccttaactgg tttgattaat ccctctttgc tgtaattatt
      481 tatcatggtt ttagtaagaa ttttatcact ttcaaatctt ttattggcag aaagcttatt
      541 gtcaattaga gaaataattt gatccatata taagtcaata gatggaatat cgtttgaagt
      601 tatatctgca ttatttagtg tttctagtat tatagtatta agttcattat tcatcatatg
      661 ttacctccat aatatatata atacactaaa ttagaaagta aaacaatatt aaggcttgta
      721 aaaaaaacat aaagtggtat aattaaatat ataatatagt aatggaaact atatttgtga
      781 atctgatttt taaaatacat aattgtagga ggacgaaatg tatcaatttt ttttaaaggg
      841 tagagaccca ataagcagtt taactcattt tataggtgct tgtttatctc ttttagcaac
      901 tataatttta gtttttcaat ctgttacatt acaggaaact tctctcttga tgatagtatc
      961 tgtatcggta tttggatttt cactaattgc tttatatagc gcaagttcgt attatcattt
     1021 tttaaagggg acaccagaac aagaattgtt ttttagaaaa gtagaccatg caatgattta
     1081 cgtattgatt gctggctctt atacaccaat atgtctgaat tttatggaaa aaaaagaagg
     1141 cataatattt gttacagcaa tatggatagt tgcatttatt ggtataataa taaaaatatt
     1201 ttggatggat gcacctagat ggttatctac cagtatatat ttacttatgg ggtgggcaat
     1261 tgtttttgat ataaatgcat tcaattcaat tcctaaggat tgccttagac tattaattat
     1321 ggaaggtgta tcttactcta taggtgcaat tatttatata attaaaaagc ctaatataag
     1381 tcctgagttt ggatttcatg aaatatttca tatttttatt atgataggtt ctttatttca
     1441 ctttttagca gttttattgt atgttttata gttttaaaag atttgattaa ttgatactaa
     1501 aattctacaa tgaataaaat ggaactgcct tatatagatt atatcttcga caataagaca
     1561 gttccttatt ataaatatta aattctactt ataataaaat tttgataaaa atagtccatt
     1621 caaaatgcct attccataat tcctttaatg tacttttcat ttacgacata tagtttgaat
     1681 ttatgtacta taattatatc ctctaaattc tctgaaacac taacaattct gacacatttg
     1741 tttctaattt ctacaggtaa atctaaatca tttgttaaat tcacatagtc atcatattta
     1801 tacataaaat catcccttta ca
//
LOCUS       DAELTA010000292         1818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.297, whole genome shotgun sequence.
ACCESSION   DAELTA010000292 DAELTA010000000
VERSION     DAELTA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.297"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(50..802)
                     /locus_tag="KPG13_003606"
     CDS             complement(50..802)
                     /locus_tag="KPG13_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG0987268.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(861..1130)
                     /locus_tag="KPG13_003607"
     CDS             complement(861..1130)
                     /locus_tag="KPG13_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0987269.1"
                     /translation="MNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLL
                     DRSMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT          651 a          307 c          139 g          721 t
ORIGIN      
        1 ttaaatataa aagctttaca attacttata aatttaaaaa gtctataagc taaactaatg
       61 tttgatatac caattttacg accataaata atgccattac taaaaatatt ggttttatca
      121 tctttgaacc tttctttata gcaactttag cacctacata agctcccatt atcatcacta
      181 atgcaaacac tatagcaatt ttataatcta cttgaccatt tatcatgaat aataacaatg
      241 aagttatatt acttgttaag tttaatattt ttgtatttgc agaagcatgt aaaaaatcat
      301 atccatatat ttttataaat ccaaaagtca aaaaagttcc tgtacctgga ccaaaaaaac
      361 catcataaaa acccattata agagccataa gcatccctaa tcttaaattt ttcttattaa
      421 cagcttcaaa attatcttct tgacctaaat ttttagaaat aaaggtgtaa atagccacta
      481 ttaaaatcat aaccacaacc aaaacattta atatctcttc acttattgat aatacacatc
      541 tcacacctag aacactacca ataatagtaa atggtactaa tttttttaaa aggttgttat
      601 taatttttcc agactttgca tatctatagg cactacttat acatcctgct gatgctgcaa
      661 atttatttgt accaatggca agatgtactg gcactccaat tgccataaga acaggcatgc
      721 ttataagtcc tcctcctcct gctatagaat ctacaaaagc agctaaaaac ccccctatac
      781 ataaaaaaat aactgaaaac ataaaattcc tccatcattc tctttcaatt tttcttacaa
      841 tatatattat ataatcttat ttaatactta ttatccagca tattctttct atattcattt
      901 ggagttatac tattaaactt cttaaatatt tgtgaaaaat aactactgtt attaaaacct
      961 actttcttac aaatatcact aatactcatg cttctatcta acagatattt tttagattct
     1021 tctatcctaa gaagatttat atactcagtt attgaaactt tatttctttc attaaaaata
     1081 gaactcaaat aatttggact tataaaaaaa acatctgata tactattcaa atttatattt
     1141 tctttataat atttttttat atattcgttt atattttcaa ttagatttcc tttatctttt
     1201 ctccaaaatg ctctattctt atcaatacac tctttggaaa attcaaataa tacattccat
     1261 aaatcttcat cactgtttac aagtgatatt tttttattat atttatttct taaataagat
     1321 ataagtttta aatcctctaa atctctaagc actgttctag acaatacaaa tatcatttcc
     1381 tgaatagtat ctctaacatc attatatctt aatttgtttg tatctattag attataaaca
     1441 ccttctaaat tcatattttt aatttctttt tcaatataaa gttctaattc aaagggatat
     1501 tcttttgaag aattattgaa catatctatc ggattaataa tatactcttc ttctaataat
     1561 ctactatttg cttttttaag tgcctcttca agtcttatat tttcttttct actcttcaat
     1621 atctcctcat cttttatact aagttgattt tcaaaaaaat tcctcttagc tatttcctga
     1681 ataaattcag acatcattaa taaattttct tttaatacat ataatctact tttaggattt
     1741 aatggaaatt cattgtaaat tttgtttatg tattttatat caataccaga actacttgat
     1801 aattcagata aattttta
//
LOCUS       DAELTA010000293         1780 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.298, whole genome shotgun sequence.
ACCESSION   DAELTA010000293 DAELTA010000000
VERSION     DAELTA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.298"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            135..1733
                     /locus_tag="KPG13_003608"
     CDS             135..1733
                     /locus_tag="KPG13_003608"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG0987270.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          683 a          220 c          275 g          602 t
ORIGIN      
        1 atttatgtaa gaaataaatt ttatataata ttttttcact aaattttttt ctttttacaa
       61 caaatactca ctaccttttg atataatatt tttatcaatt attattatat tcaagttttg
      121 aaaggagtat taaaatgagt ttattagttt tcggacataa aaatccagat acagattcaa
      181 tatgttctgc aatttcatta gcacatttaa agaacaaact aggaataaaa gcaacagctt
      241 atgctttagg agatatcaga aaagaagcta aatatgcctt ggattacttt aaggtagatg
      301 cacctgaaat tttagataat gttagaatac aagttagaga tttaaattat gataaagtag
      361 tacctttaac accaacttca tctatattag aggcttataa ccttatggat gaaaaaaatg
      421 ttaaaacact acctgtaact tatgatgatg gaacttttgc tggtatcatt actatgaaag
      481 aaatagctaa aaatctactt catcaacact ttacaactat acacacatca ctatcaaaca
      541 tttgtaaaaa ccttaatggt actattcttg tggactgtgg tgaagatata aaaggtagag
      601 tagtcacttt atcatttgga atggagactc taatagagac tttaaaagaa ggagacatag
      661 ctattgttgg agatagatat gatagtattg aatatgctat tgatacaaaa gttaaacttt
      721 taatacttac taatcataca gagatttcaa aagatttgct agctcttgct aagataaatg
      781 gagtgtctgt tgtttctgtt gaaagtgaca attataaaac ttctaatgta ataaatcaat
      841 gtagtttctt agactctata gaagctacag aaaatttaat tacattcaaa gaagatgatt
      901 atatggatga aataaaagag attatgctag aaacaaactt cagatcttat ccaatattag
      961 atgatgataa taagttttta ggtttagttt caaaaggtca tctattaaac ccatctaaaa
     1021 agaatgttgt tttagttgac cataacgaat atgctcaaag tgctgatgga attgaacaag
     1081 caaacatagt tgaaataatt gaccatcata aacttggtgg tatttctact gatgttccta
     1141 tgtcatttag agttatgcct gttggatgca atagtactat aatttatcaa atgtacaaag
     1201 aaaataatgt tgagatacct tatgaaatag ccgggcttct tttatctgct atattatcag
     1261 atacattatt atttaagtct ccaacaacaa ctgatataga taagaaagct tgtgaagaat
     1321 taagtaaaat agcaaaagta gacatggaaa aatacgctat ggatatgttt aaatgcggta
     1381 cttctttaga tgaatatact atagaagaaa tagtaaatat ggactttaaa gaatttaata
     1441 tgagtggaca tagagttggt attggacaag tatttacctt agacatagac tctatatttg
     1501 ctaaaaaaga tgagttttta tcttatataa attcgactga ttatgacaaa ctaattcttg
     1561 cggttacaga tataatcaaa gaaggttctt atttaatcta taaagcagaa gacagcttaa
     1621 ttgctaacgc atttggtgtt gaaggtgttc aaggtacatt tgcaccaggt gttgtatcaa
     1681 gaaaaaaaca attagtccct aatttaacta ctgcaatcaa aaattttaaa taatataaaa
     1741 tttttaaata acgaaaactg tctcaaatct atatttgaga
//
LOCUS       DAELTA010000294         1732 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.299, whole genome shotgun sequence.
ACCESSION   DAELTA010000294 DAELTA010000000
VERSION     DAELTA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.299"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            302..1594
                     /gene="brnQ"
                     /locus_tag="KPG13_003609"
     CDS             302..1594
                     /gene="brnQ"
                     /locus_tag="KPG13_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG0987271.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          627 a          213 c          300 g          592 t
ORIGIN      
        1 taataaatcg aataatctaa aaagtcttaa tattttaatt attcggaata gttaatttta
       61 tcaagaaaaa ttagagaaaa aacaaaaaac caactatgtt taaagtatac aggttgattt
      121 tgtttaatta agaaagttgt attaaatatt gtgaagattt tataaattat gaaacataat
      181 tgacaaattt aagtcaaaaa tgtataatta aataaaatct cgtcaaaagg ttttcattta
      241 attaagagat tggacttatt ttttagtaaa aggtagtaaa attcaaatgg gggtgcttta
      301 aatgggtgag aaacaaatca attttaaaga tgtaattgta attggatttg cactatttgc
      361 aatgtttttt ggtgcaggga acttaatctt ccctccatat ctaggagttt tatctggaag
      421 tagttggcta gtagctttca taggattcct ttttgcagat ggtggtttgg cattattagc
      481 agttatagca gcaactaagt tcaatggaga cacatcgaag atgttctcaa gggcaggcaa
      541 aggattaagt attgcattag gatgtgcaat ggtaatctgt atagggccac ttttagcaat
      601 acctagaaca gcagcaacaa cttatgaaat gggtatatta cctacaatag gttctggaat
      661 aagcccagta atattctcta tagtattctt tgctctagta ttggttttaa caataagacc
      721 atcaaaagtt gtagatatag ttgggtcaat attaactcca gctttactta tagctttagc
      781 agtattaata gttaaaggta tcgtatctcc attaggagaa ataagagaca gttctttaat
      841 acaaaatgta tttgcagaag gtataactca aggttatcaa acaatggatg cattggcagc
      901 tagtgtattt gcatcaatta taatcatgtc agtaatagct aaaggttata caggtgagaa
      961 agaaaagatg aaagccacaa taagtgcagg tgtaattgca gtaataggaa tggcattagt
     1021 ttatggtgga ttatgttatc ttggagcaac tgtgtctcaa caatatggac aagatgttca
     1081 acaaacagct ttaatagttg caataacagc agctttactt ggaactacag gtaaaatttt
     1141 attggctatt atagttgcat tagcatgttt aacaacagct ataggtcttt cttcagcagc
     1201 tggtcaatat ttctcaacac ttacagatgg aaaattaaag tatgagcata tagttatagt
     1261 agtttgtgta tttagtgcaa tcatatcaaa ctttggagta agtacaataa tacaattctc
     1321 ttctcctata ctaagtatgg tatatccagc aactataact ctagttatac tagcattatt
     1381 tagtaataaa attaaaaatg acaacgtgtt caaatgtgct gcatacatgg cattattagt
     1441 aagtgtatta acagttgcaa caagttttgg tgtaaaaatt cctctagtta atagcttacc
     1501 atttgcttca ttaggattta actggatagt tccagttgta atagcaggaa taataggaaa
     1561 ctttattcca tctaaaagca gtaaaactgc ataataataa aaactaattg ataattatgg
     1621 aattgccttt aaattatata ggcaattcct ttttatattt aaaagaatta ttaaattatt
     1681 gtagataaaa gaacattatt aatcctgttt gataaattta aaaatttatt tt
//
LOCUS       DAELTA010000295         1728 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.300, whole genome shotgun sequence.
ACCESSION   DAELTA010000295 DAELTA010000000
VERSION     DAELTA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.300"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(72..638)
                     /locus_tag="KPG13_003610"
     CDS             complement(72..638)
                     /locus_tag="KPG13_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG0987272.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            804..1325
                     /locus_tag="KPG13_003611"
     CDS             804..1325
                     /locus_tag="KPG13_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG0987273.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT          718 a          196 c          257 g          557 t
ORIGIN      
        1 ataaaaatgt tatataaaaa tgagtatata gcattttaaa gagtaataaa ccttacataa
       61 taaatgattt attaatatga actagaagta tttttactat attgttttat aaaaccttta
      121 ggcattttac ttataaaata taccaccata atacaagaca aaggtttgtc tactatatta
      181 ctaagaatcc ttggaaagaa cgcagcagta aatatcttct gaccagactg aactaaaaaa
      241 ccaaccaata aatcagcacc acttccactt aaaccaccaa acaataaaac tgaaatagga
      301 gtaccaatta aaggacaaac aatagataaa attactcctg taataatagc ttctttataa
      361 ccaaaacctt ttttcttaga aatatatcca acaataagac caaggacaat atttacaagg
      421 gcaaaaggta tatttgtagg acctgatata acacctaaaa ctaagtttgt acatccacca
      481 ataagagcac cccaaaaagg acctaatacg acagccccaa gtacagtacc aactgtatct
      541 aaaaataata atggtatatt tatcatagat ataaccatac ctaagacaat attaataact
      601 atgcacattg aagaaaatgt gactttttta gtatccaatt ataatacctc ctaaaaaaat
      661 atatacattt atcatagtat agattataaa agattactaa ttgtatattt ctatatagaa
      721 tattggaaat atagaacaaa taaataatat aattatcaaa atatgtagta tcattaaaat
      781 taagaataac taaggagatt attatgaaaa gaaatagaga gattgtagta gtatgtcatt
      841 gtatattaaa ttgtaattct aaagtggagg gattgtcaga atttaaggga gcacaaaata
      901 tagcgataaa tttaataaat aaagggtatg gaataattca actaccatgc ccagaaatga
      961 ttatgtatgg aataaatagg tggggacatg taaaagaaca gtttgacaat ttgtttttta
     1021 gagaacaatg taggcaaatg ttaatacctc atgtaaggca gtttgatgaa tatttaaaaa
     1081 atgattatga tataaaaagt atcattgcaa tagatggcag tccaagttgt ggttataata
     1141 agacatgtag ttctaaacgt tggtttggtg agttaagtgg ttgcaatgaa ttgatggata
     1201 aaataaagga tatacaaatg gtggatggga aaggtatttt tatagaagag ctagaaaagc
     1261 tacttaaaga atataattta gagataccta tagttggatt tgacgagctt agagaagata
     1321 agtagagata taaatattta ttgtctcatt atgaaacacc attatatttg gcagaaaaat
     1381 atgactgatg gacagtacag gaaccatttt taagtggagt aattagtact tataaagatg
     1441 tatccactag ctgaaagaaa atgtagaaag gtaataaatg aattaagaag tagataatat
     1501 atataaagga gtgtctaaaa gtgaactttt tagtttatga gacactcttt ttttatatta
     1561 aatcaagtat attaaatcaa atatatttta atcattttag caatgaaaaa taaatttatc
     1621 tatattttga gacaacatct tttttagttt tatttatttg ttgtcgtttt tgacttggta
     1681 tacaattgta tattttctta aataatttca taaattattt ataattat
//
LOCUS       DAELTA010000296         1702 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.301, whole genome shotgun sequence.
ACCESSION   DAELTA010000296 DAELTA010000000
VERSION     DAELTA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.301"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            33..1166
                     /locus_tag="KPG13_003612"
     CDS             33..1166
                     /locus_tag="KPG13_003612"
                     /inference="COORDINATES: protein motif:HMM:NF025330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HBG0987274.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASTLGSPFVSTD
                     SFSTTKTKIQTFKSTLATNLTKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGVTSALQQYPPCVAVENYIYFLCNNSLGLSLLRYDTLTNVNSVLAKPSVCDDAL
                     MVYYDGYIYRIGGYSNSSANSTCQKYNIKTNTWSYISNMITPRGGTCGNTYSNEIHVL
                     YGYTDTNSNSTASAISEYYKISTNTWSTKTSFPPSRDRRLFVCAKTGTYYFCITGATD
                     PNNIWETYINRYDAGNGALTKIEGWYKSFATSINDMLYTVYYGDTYLYVKGYNQNGRV
                     FSKEYVKKNSTFPYNPLLKAVSVKNKYIYYYENNMYKCFIPEL"
     gene            1184..1549
                     /locus_tag="KPG13_003613"
     CDS             1184..1549
                     /locus_tag="KPG13_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987275.1"
                     /translation="MIKIWDKKEKINGVSAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKNLTALETAEKYLEMHKKQEEDIKAHEELEKNSKNATIATHEEVK
                     ALREETATLTFAMMEKELI"
     gene            1550..1684
                     /locus_tag="KPG13_003614"
     CDS             1550..1684
                     /locus_tag="KPG13_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBG0987276.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIVGEDYIV
                     "
BASE COUNT          725 a          199 c          276 g          502 t
ORIGIN      
        1 aaaggatact taaaaaagga ggcaggaatt taatgactat aaaattagaa gaagattcaa
       61 gtataaaaga agttatggac tcttttgaga atataaaaaa tgaccttcaa aatgctaaag
      121 acaacttagc tagtacatta ggaagtcctt ttgtttcaac tgattcattt agtactacta
      181 aaacaaaaat acaaactttc aagagtacat tagcaactaa tttaactaaa aaaggtgttt
      241 caacttcttc aaatgaaacc gtccaaaata tggtaagtaa agttgatgat attgaactag
      301 gaaatacaga cataccaaac tggtataaac ctaaaaatat aatttttgat ggagttactt
      361 cggcattaca acaatatcct ccatgtgtag cagtagaaaa ctatatatat tttctttgca
      421 ataacagttt aggtttatcg cttttacgct atgatacatt gacaaatgtt aatagtgtct
      481 tagcgaaacc atcggtttgt gatgacgctt taatggttta ttatgatggt tatatttaca
      541 ggataggtgg atatagcaat agttcggcaa attcaacttg ccaaaaatat aatataaaaa
      601 caaatacttg gtcgtatatt tctaatatga ttacacctag aggtggaaca tgcgggaata
      661 cttattctaa tgaaatacat gttttgtatg gttacacaga tacaaatagt aattcaacag
      721 caagtgctat cagtgaatat tataaaatta gtacaaatac ctggtcaact aaaacaagct
      781 ttccgccatc tcgagataga agattatttg tatgtgcaaa aacaggcacc tattattttt
      841 gtataacagg agcaacagac ccaaataata tttgggaaac ttatataaat agatatgatg
      901 caggaaatgg agctcttaca aaaatagaag gttggtataa aagttttgca acaagtatta
      961 atgacatgtt atatactgtg tattatggcg atacatatct ttatgtaaaa ggatacaatc
     1021 aaaatggaag agtattttct aaagaatatg ttaaaaaaaa ttctacattt ccatacaatc
     1081 cactacttaa agcagtttca gtgaagaata aatatatcta ttactatgag aataatatgt
     1141 ataaatgctt tatacctgaa ttataaaaag gagaaaataa aaaatgataa agatatggga
     1201 taaaaaagaa aaaataaatg gagtttcagc agaagaaatt ttaaaaggta attatgattt
     1261 tcaaacaagt gaagtatttt taatacttga tgattataac agagttacaa atatagagtc
     1321 agttaataca attaaatcta tatataaact agataagaat ttgactgctt tagaaacagc
     1381 agaaaaatat ttagaaatgc ataagaaaca agaagaagat ataaaagcac atgaggaact
     1441 agaaaaaaat tctaaaaatg caactatagc tacacacgaa gaagtaaaag cattaagaga
     1501 agaaacagca acattaactt ttgcaatgat ggaaaaggag ttgatataga tgtggtataa
     1561 aataataaaa aaatactatg aggatgaatt atggactaaa gagcaagtta aaacagcagt
     1621 agaaaagaat aagataacaa caatagaata taaaaatatc gttggagagg attacattgt
     1681 atagtccttt tttaatttta ta
//
LOCUS       DAELTA010000297         1684 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.302, whole genome shotgun sequence.
ACCESSION   DAELTA010000297 DAELTA010000000
VERSION     DAELTA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.302"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            146..892
                     /locus_tag="KPG13_003615"
     CDS             146..892
                     /locus_tag="KPG13_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG0987277.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            953..1627
                     /locus_tag="KPG13_003616"
     CDS             953..1627
                     /locus_tag="KPG13_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG0987278.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
BASE COUNT          679 a          163 c          283 g          559 t
ORIGIN      
        1 tgtttaaata tatttttata ttttgtaatt tcaaattaat aaataattag atttgataaa
       61 caaaaaataa aaatgttgat tatattaata aactttaata taatatattt atgaaaaatg
      121 taaactgtga aatggaggta gtaacatgaa aattgaaaat ctttctattg atgatattaa
      181 acgaggctat atatttaatg atgatttaaa ttgttatagt tgcattaaat gtacaaaaac
      241 atatgaggta ggagaaatat ataaaatagg taatagattc tttgaagctt caaaagctat
      301 tgaacttcat atggaattag aacatagtga ttatttagag caattgctta atagtgattc
      361 aaaatacaac acattgactg aaaatcaaaa acaattattt caattattta ttagtgattt
      421 accagataag gagatagcaa aacaggttgg agtatccaca tctactatac gccatcaaaa
      481 atttattttc agagaaaaag caaaacaggc taagctctat ttagcactat atgaatctgt
      541 atttgaagat aaatctaata aaagtagtac tataatacca atccatgaac atgctcgaat
      601 ggtagatgaa agatatgtag taactgaaga agagagaaaa cacattttgg atacatcatt
      661 tgaaagtatg aatcctttaa aattaaaagt attttcttca aaagaaaaga aaaaggtcgt
      721 aattttatct aagattttgg aacaatttga acgtgggaag aaatattcag aaaaggaagt
      781 taatcagatt ttagaatcta tatatgatga ttttgcaaca attagaagat atctaattga
      841 gtatggtttt atgagtagaa ataaagaatg tacaaaatat tggttaacat aattaaaata
      901 ataaatttta ctaatgtgta atgttatttt tatctagggg gaggagtcaa atatgaatga
      961 tgaattaaaa tctattttat acaataaagg tgtggacatt gttcgctttg ttgatatatc
     1021 tgaatttcca ataaatcaaa cgcaaggttt ttctaaagca attttatttt gtatagggtt
     1081 atcaaaaaaa tttattacag atatatacaa taacttacct acagatagtg atgagttttt
     1141 agaaaaagag gagaaagcag aagaattagc tgactggatt tcagaatata ttcaaaataa
     1201 aggatataga gcgtattctc aatcggagaa aaacaatcta gaacatggat attttgaaaa
     1261 agcctatatt aatccagaaa tgcaatcagg gattagtcct ttaccacata agacaatagc
     1321 aaatattagt ggcattggat ttatgggaaa aaataatttg tttgtaacag aggaatacgg
     1381 atgtgctttt agcatgtgta cagtacttac agatgctcca atttctgttg aaagatatcc
     1441 tttgattgat tcaaaatgta tggattgtaa tgtttgtgta gaaaactgcc ctgcaaaagc
     1501 tatacatgga aatgagtgga ctttgccagg aaaaagagag tctattattg atgtttcaaa
     1561 gtgtttttgt gtattgaagt gtatgatgag ttgcccatgg tcattaagat atgcaaatca
     1621 aaaataattt tactagagaa ggtattaaat tgaatattat aaaattttta aaatgtaaga
     1681 ttat
//
LOCUS       DAELTA010000298         1631 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.303, whole genome shotgun sequence.
ACCESSION   DAELTA010000298 DAELTA010000000
VERSION     DAELTA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.303"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            116..589
                     /locus_tag="KPG13_003617"
     CDS             116..589
                     /locus_tag="KPG13_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG0987279.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(958..1515)
                     /locus_tag="KPG13_003618"
     CDS             complement(958..1515)
                     /locus_tag="KPG13_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG0987280.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          597 a          213 c          173 g          648 t
ORIGIN      
        1 taatataaat tatatattat cataatattc taaatttata aaacaaattg tattgaattg
       61 aatttattta agatatataa ttataattaa aataataata aaggtgttgt ttataatgtt
      121 caaagaatac ttaataagag aattaaaaga agatgatatt aaaaatgcat tagacttaat
      181 ctggaacgta tttgaagaat ttgaatctcc agattattgt gatgaaggaa ttacgacatt
      241 tcatcaattt atagaatata actctattat tgaaaaaatc aaagaaaaaa ctctcttctt
      301 ttggggttgt tttgactcta aaaaacttat tggagtctta gctattagaa atttaaatca
      361 tatatgtatg ttatttgtag ataaaaacta tcataaacat ggtgttgcaa aaaaattaat
      421 gactacaggc atacaatact gtatttgtaa taaatttaat ttaactgaaa taactgtaaa
      481 ttcatcacct tatgctgttg aagtttatca taaattagga tttaaagata ctgctgttga
      541 gcaaattgtt gacggtatac gttttacacc tatgaaattg ttaatataat aaattaaata
      601 aaaagcaaag tctcttaaat attttatagt tactttgctt ttaaaccaag atttaaaata
      661 atactatctt ttttgttttt taataatata ctttaaaatc cttactaata tgaactataa
      721 tttatttttt gatatccttt ttgattaaca tataaattaa ttcaattatt aaacataaac
      781 tatgttgttt ctctttatat ctcttctttg taaacaatat tgggcatttg aaaataaata
      841 ttcttaaaat atactgttct cctgttgatt ttcctttggt acaaatgata aaaatttaat
      901 ttacatgccc cataatgaag tgttcaataa ataagttaaa ataatttatt tacctcctta
      961 atcatatttt ttatgaatac ctaataacac agtatcaata gaatcggcta aaccttttat
     1021 agcttcttct ttactcaaaa gtgatttccc aagttgcaaa gtcgaataac catgaagtac
     1081 actcattatt aagtttaata cttcacctgt attctttgta tttagattac aagaaagaat
     1141 aagtttttct aataaggatt tatattcatc aaagatttct aatgtttcat tatttccatg
     1201 ccaagtagcc cactgaattg tttcataaac accaggatta gaaattataa aattaaagta
     1261 tgctatactt atacacttta tagctgtatc tccaaaattt ccaatagtcg cctttatcat
     1321 ttgactattc atctctctca ttcctttatg agctacttca agtaataaat catctagact
     1381 ttctatatga ttgtacaaag atggtgtacg tattcctagc ttttctgcaa taacttttaa
     1441 agaaactttg ttaagcccat ctttattaac tatatctgat gtagcttgta taactatttc
     1501 tctactaaca cgcatgattt tccctctttc atttaaaatt aattacttta ttagtttagc
     1561 taatctaatt agtttcgtca attattgtaa tttataaaat tctttatact attataaaat
     1621 cttgacagtt t
//
LOCUS       DAELTA010000299         1612 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.304, whole genome shotgun sequence.
ACCESSION   DAELTA010000299 DAELTA010000000
VERSION     DAELTA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.304"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            5..757
                     /locus_tag="KPG13_003619"
     CDS             5..757
                     /locus_tag="KPG13_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG0987281.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            744..>1612
                     /locus_tag="KPG13_003620"
     CDS             744..>1612
                     /locus_tag="KPG13_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG0987282.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSG"
BASE COUNT          682 a          186 c          248 g          496 t
ORIGIN      
        1 ctatatggat aatattatta atgaattaca aaatatagga tttactaaat acgaatctca
       61 aatatacata tcattattaa aggaaagccc tttaacagga tatgaaataa gtaaattatc
      121 aggtgtacca caatcaaaag tctatgaaaa tataacaaag ctattaaaca ataatataat
      181 tatcaatgtt ggaactgacc ctattaagta cataccaata aaccctaatg aattattaaa
      241 aaataagaaa aaagaatttg acaattctat ggataatcta aagaatagct tacagatatt
      301 aaataaaagt aaaagtgtag aatacgtatt aaacataaaa ggagataaaa atatattaaa
      361 aaaagcaata gaaatgataa aaaatgcaaa acaagaaatt ataatttctt gcccttatga
      421 agaaattctt gaattaaaaa aagaactcct aattgcttac aaaaaaaata ttaatattga
      481 aatactactt ttagaaaaca aaagtctgaa aggtcttgat aatattcata ttcatggagg
      541 taataaaact acacttgaaa ataaaatgct ttacaaagga attattttga ttgtagataa
      601 tgatgaaata ttgacaggaa acttatcaga aggcagtgag gccatttcaa tttggacaaa
      661 aaactctaac attttatata ttggagttca atacataaaa catgaaattt atatttcaga
      721 acaaataaaa ggaggttata aaaatgaaca aaattaagat taaacaagtt gatgctttta
      781 caactatacc ttttggtgga aatcctgctg gtgtcgttac tgatacctct aacatttctg
      841 atgaaattaa gcagaatgtg gctaaagaaa tgaatttatc agaaactgca tttgtttcag
      901 agtcaaatat tgcagatttt aaagtacaat ttttcacacc aggtttagaa gttgatttat
      961 gtggtcatgc aacaataggt actttccatg cacttttgga agaagggaag cttaatacta
     1021 ataaaagtat attttatcaa gaaacaaaag ctggtgtatt atctgtagag cttaagaaaa
     1081 taaataatga aaatatattt atgatggaac agaatacacc tgaatttgaa aatttagata
     1141 attgcagaaa agaacttgca caaatgatag gtgtgcatga agatagactt cttgaatatc
     1201 ctattataaa ggtaaatact ggactttggt ggcttgtatt tggtctaaaa tctttagatg
     1261 atttaaaaaa tatctctcca gatttaaata aaatcaaaaa atttagtggt gataataata
     1321 taattggtat aactccattt tgtatagaaa ctatagataa aaagtgtgac taccatttaa
     1381 gatctatagc accatatgtt ggagttacag aagaccctgt atgtggaact gggaacgctt
     1441 gcgtatcatc ttatatagta catcacaata ttttagataa aactagtttt attggtgagc
     1501 aaggtaactt tataaacaga cctggaaagg tatatgtaaa tataactaga gaaaacaata
     1561 acataacttc tgtaaaaatt ggaggaagtg cttatactgt cttaagtggt ga
//
LOCUS       DAELTA010000300         1611 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.305, whole genome shotgun sequence.
ACCESSION   DAELTA010000300 DAELTA010000000
VERSION     DAELTA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.305"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            315..1355
                     /locus_tag="KPG13_003621"
     CDS             315..1355
                     /locus_tag="KPG13_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG0987283.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT          485 a          205 c          262 g          659 t
ORIGIN      
        1 tataagataa tattttacgt tctaataatt attttttcca tattaaattt attttttatc
       61 aatcttccta tacaagactg cattatttat atataattgt attataaagt ataagtttaa
      121 tttacatttt atacgttaag cttactttat atatttttat agttattttt atttttagat
      181 gcaatataaa accaattaag atttaaaata acttttgtta tgttgacatt tatttaggta
      241 atcatttatg aattaggata acgttatcac aacaaaagtt tatgtcctaa aaaaaataca
      301 aggaggattt tcttatgtca gaaattttat taggaacagg tttactttta ttggttctat
      361 gttgtttcac aatttttagt tacaaggccc catatggtat gaaggctatg ggagctctag
      421 caaacgctgc atgtgctact ttcttagtag aggcattcaa cctttcttta cttggtgatg
      481 ttttaggtat ggaatttttt agaagtgttg gagctgctaa tggtagtctt ggtggagttg
      541 ctgctgctat acttgtgcct ttagccttag gtgtatctcc agtttatgca gttttaatag
      601 gattatcttg tttagatttt tctatattat ctggattcgt tgctggatac tttgtttctt
      661 tcttaattaa gtttatagag aaaaaagtgc cacctggagt ggatttagtg gttgtaattc
      721 ttctatgtgc accactatct cgtcttatag catcaaatgt agacccatta gttaatagta
      781 cactactaca aattgggcaa gttttgatag aagctaccaa cattagccct ataataatgg
      841 gaattatact aggaggtcta ataacagtag ttggaacgtc tccacttagt tcaatggctc
      901 ttacatctat aattggtctt actggtcttc ctatggcaat aggagcatta ggtgcttttg
      961 gttcagcttt tatgaactca acactatttg caaaattgaa attaggttct aaaaaagata
     1021 ttatatctgt tgctattgaa ccattaaccc aatcagacat aatttcagct aatccaatac
     1081 ctatatattc tactaatttc tttggaggag ccttttctgg tatcattgta gccttgatgg
     1141 gtcttgtaaa catgacacca ggtacagcta cacctgtggc tggttttgct ataatgtttg
     1201 cttataatcc agttcaacaa gttcttataa cagctggttt atgtgcttta ttcaatgtaa
     1261 ttgcaggatt tttaggttca aaaatattca gtacctttaa agttcttact gctggtgaaa
     1321 tacgtggtac tgatgtagaa gaagaaattg cttaaaaaac aataagattt ataaatttat
     1381 atctcaagta gtagagtttt atattgtagg caattttatt aattgcaaac gttttatttt
     1441 ttatttatgt ttatgattat tatgtgtgtt gaatatttag ataaattttt atttattatt
     1501 tttatttaga tttttgttta gttttttatt tggttgatat attaggaata aaaattagta
     1561 tagtttatta ctagttagat ggcataactt tttagataca taaaaaattt t
//
LOCUS       DAELTA010000301         1580 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.306, whole genome shotgun sequence.
ACCESSION   DAELTA010000301 DAELTA010000000
VERSION     DAELTA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.306"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(158..492)
                     /gene="rnpB"
                     /locus_tag="KPG13_003622"
     ncRNA           complement(158..492)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KPG13_003622"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            624..1301
                     /gene="srtB"
                     /locus_tag="KPG13_003623"
     CDS             624..1301
                     /gene="srtB"
                     /locus_tag="KPG13_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG0987284.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(1461..1535)
                     /locus_tag="KPG13_003624"
     tRNA            complement(1461..1535)
                     /locus_tag="KPG13_003624"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1501..1503),aa:Thr,seq:tgt)
BASE COUNT          596 a          247 c          194 g          543 t
ORIGIN      
        1 tcattttact ttttcttttt atatctatct tatattatac catttttaat atctgtacta
       61 acttatatta caatattcct caaaatttta aaagtagatt agacaaattt acaaataaaa
      121 aaacatcagt ttaaactgat gtttttgact atcgaaataa ttaagcaagt ctataagcgg
      181 agttctgtat tagataatca tctttctagg cctattgtca ccaataggct caagcgacct
      241 acctaccgga gagtacgagc agcactcttt attcctatct tggtcttgct ccaggtgggg
      301 tttacatagc caactagtcg cctagctgct ggtgagctct tacctcgcct ttccatcctt
      361 accacaatag tggcggttta tttctgttgc actatcctta ggatcgctcc cactaggcgt
      421 tacctagcac cctgctctat ggagctccga ctttcctcat gtaagattac atgcgattat
      481 ctggcttact taatatttat tttacttagt tatgttaaca caaattcttt tacatgtcaa
      541 agtaatatta tactaaaaat ttaaatttat tcaaaaatag tgtaatatag atatataaac
      601 tacatttaac tcgaggtgat ctattgaaaa aattatatag aatagtaatt aacattatac
      661 tagtattagt aatactttac agtggattta atatatactc aaaacttacc aaatacaatc
      721 atgatactaa aatatcctca gaacttcaaa aaaaagaata taaaaaagag gatttatcaa
      781 agattaacag tgattttaaa ttttggcttt ctgttgagaa tacaaatata aattatccag
      841 ttgtacaatc taaagataat tcttactatc ttgacaaaga tttttataaa aaagattcta
      901 tttctggtac actgtttatg gattatagaa ataaatctat tgatgacaaa aatatcataa
      961 tatatggaca taatatgaaa aataaaacta tgttcaacaa cctaaataag tttaaagatg
     1021 ctgacttctt taaaaaaaat aataaaataa aaataacctt gaatggaaaa gaatttttat
     1081 atgatgtttt ttctgcttat attgtagaat ctgattatga ttatctaaaa acaaatttta
     1141 acaatgaatc tgattatcaa aattacataa atgatattac ttctaaatca ttgtataaat
     1201 ctcctattaa ggtaaatagc aatgataaaa tagttacact ttctacatgt acctatgaat
     1261 ttgatgatgc cagaatggtg attcatggta gattgattta aaactcacag acacagttta
     1321 aaacaataat tttaaactgt gtcatctttt atacctataa tttgtaaatt agaccactaa
     1381 aagtatttaa tacaaaataa caaatacatc aaacaataaa aaacaataaa aaaagactat
     1441 gctaaaaaca tagtctttac tggagctggt gataggaatc gaacctacaa cctgctgatt
     1501 acaagtcagc tgctctaccg ttgagccaca ccagcacacc tattttattg taaattagca
     1561 aaactaattt ttaaaagtta
//
LOCUS       DAELTA010000302         1515 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.307, whole genome shotgun sequence.
ACCESSION   DAELTA010000302 DAELTA010000000
VERSION     DAELTA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.307"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            118..372
                     /locus_tag="KPG13_003625"
     CDS             118..372
                     /locus_tag="KPG13_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987285.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            417..740
                     /locus_tag="KPG13_003626"
     CDS             417..740
                     /locus_tag="KPG13_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987286.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1299..1493)
                     /locus_tag="KPG13_003627"
     CDS             complement(1299..1493)
                     /locus_tag="KPG13_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG0987287.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          617 a          137 c          211 g          550 t
ORIGIN      
        1 aactataaag gttacaattt tggaaataga tgtaaatata tgttgtattg tataataaga
       61 gtaatataaa atctagtaaa aataatcaaa taaatttagc ttatgagagg gataactatg
      121 gaaaataagg attttaaatt aacaatatgt atatttgctt ttgtaattgg agtagtagta
      181 ttattctgtt gtataggttt aggtgaattt agtgcagagc atattattca acaaaatggt
      241 ggaatcatgg atactgacca atataatatt tatctagacc aatctatttc tcaatataga
      301 aattttggtt ctatattagc tctgttagga ggaataggga tattgtttga taaatatatc
      361 agaaaatact agagattatg ttttgtctaa aataaattct tatgaggaga taaaaaatgg
      421 caacagaaat atttactgta aaaattacac ccttagaggc acaaaaaatg ataaaagaaa
      481 atcttgatgc agatttagtt tttagtgatt cttataattt aggagatgat aaatttatac
      541 taattacaac ttttgagaag tattatgcaa gaaataatag tgatgcagga ttaatagtag
      601 tttatgaaaa taccacagga aaaacagtta ttaaagctac ttcaacaggc agtgcaattg
      661 gaattttaaa aattgattgg ggtgcaggtg gaaatttact taaaagagta aaaaacatac
      721 ttgctgatta tattatttaa ttattttatc taactgataa tatgtcaaga aaatctcgta
      781 aaagaggtta agaacatttt tataatatat agataaataa gaatttgaag gtaaagtttt
      841 taaggtataa agaaaaataa tataataaaa cgaattggct ttataattct ttataaggca
      901 aagagcacat ttctttatat aattgttgga tattaaataa tatgtaaata caccatttac
      961 atataaaagt ttaattaata tgttaagaga aaatgaaatg gaaattacta aaaaatattt
     1021 ttaaattgga taatggttga tattatgttt atacaaaaat catgtagaat tttatatatt
     1081 ttaaatatga tagtttaaaa ttatgaaaga atgataaatt ccagtttgta gggtaatttt
     1141 gataaataat attaataagt atgagtaaaa agtaggagca tattaatata atgtccctac
     1201 ttttttatta ttaaaattgt ttataatatc aacactacat taaaacacaa cctaaatttg
     1261 aaaaacaaat agtttagaaa tatgtacttt tttatctatt atattctttt aaatcgaatt
     1321 ttttcatctg aacataaagt ctcaaatcta tatgcgtctg tcatatcata aaaaccaaaa
     1381 aatataaagc catcacgaga agtaaattca tattgtatat tttcaatttt caacatgttt
     1441 tctacttcgc ttattttttc gctatatata atttctacca tataatttat cattgtagaa
     1501 tccctcctat taata
//
LOCUS       DAELTA010000303         1486 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.308, whole genome shotgun sequence.
ACCESSION   DAELTA010000303 DAELTA010000000
VERSION     DAELTA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.308"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(194..1072)
                     /locus_tag="KPG13_003628"
     CDS             complement(194..1072)
                     /locus_tag="KPG13_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG0987288.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(1089..1406)
                     /gene="trxA"
                     /locus_tag="KPG13_003629"
     CDS             complement(1089..1406)
                     /gene="trxA"
                     /locus_tag="KPG13_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG0987289.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
BASE COUNT          493 a          271 c          145 g          577 t
ORIGIN      
        1 aaattttgga gtatattata aataattcca atttttacta atataacttt gagttttatt
       61 aataaaagta attacatata gattttaaaa tacttttatt aataacttaa aaataaaaaa
      121 ctgtcttgaa ataataaaat tatttcaaga cagtttttta taaatatctt taaaaagtta
      181 atcaatataa atattaagcc ctctttaatt tatctaaata gtaaacagca tttaaagctg
      241 ctatttgccc ttgaccagct gcttttatat aagaataggg ttttccaaca caatctccag
      301 cagcaaagca tccatctata ctagtcttca tattattgtc aactttaata tgaatgccat
      361 caatctctat tccaggaacc aatgcacttg gagccgtact atcttttata acgaatactc
      421 catcaacttc aatgtggttt tctttaaaag atactttgtt tacaagttta tctccatcta
      481 tctgtactgg tctgtcatgt ataacttcaa tagaatcatc taggttatct gaacttctca
      541 taagattatc ttttttgtac ataggtataa aataagtctt tgaagtgagt tcacttaaaa
      601 aatttgcttc ttctttagat tcttcattat atcctataac tgctaccttt ttatttctat
      661 aaagtggtgc atcacatgta gcacagtagc ccacacctct tcctagaaat tcttcttcac
      721 ccttgattgg tctagtatac tctaccccag ttgcaaggat tacagtagta gcttcataca
      781 tatcatttcc agacattatt gtaaagtatt ctcccatagc ataaatatta tttattcttt
      841 tattttcaat tgaaatatcc atactatcta tatgtgattt aaacttgtct tttaattcat
      901 caccacttat atcataaaaa cctaaataat tatctattga aggagcttta acaagcttat
      961 tacttaaatt atcattacca aacatgataa ttgttttatt tcttattttt gcatttatag
     1021 cagcagacaa tccagctgga ccactgccga ttatagctat atcatatctc ataatactca
     1081 cccatttttt ataaatatga ttttacttgc atttcaattt tgttctttgg catgaatcca
     1141 attaaagttt ctactggttt tccatcttta aatataatca tagttggtac tgtagataca
     1201 ttgaattgtt gagctatttc taaactctca tctatatcaa cttttacaaa tctagcttta
     1261 tccttcatct cttcacctaa ttctgcaaat acaggtccca acatgttaca aggtccacac
     1321 caagttgcaa agaaatcaac tacaactact cccttactac cttcaacact acttctaaat
     1381 tcagatgtat ttataacttt tgccattttt aaatcctcct attaaacttt attatcccta
     1441 tataattata ctatgaaatt tataaatata aatttacttt tataag
//
LOCUS       DAELTA010000304         1481 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.309, whole genome shotgun sequence.
ACCESSION   DAELTA010000304 DAELTA010000000
VERSION     DAELTA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.309"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            274..1479
                     /locus_tag="KPG13_003630"
     CDS             274..1479
                     /locus_tag="KPG13_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0987290.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          566 a          190 c          209 g          516 t
ORIGIN      
        1 taatgctaag taaacctgtt tatttcataa aactaatata tattctatgc tctattttcc
       61 tttttattct atatttaaat tttttattta caagaatatt tttaatataa catatttgat
      121 aaaattaatt tacatttctt tcacttgtaa tttttgcaat cttgtattag attaatatta
      181 aaaataattt atcaataacc ttattcacaa agacagtttt tatatatcaa aatataatat
      241 aaatactatg ctacatttta ggaggatttt attatgagca aaaaagtatt aataatgtct
      301 gcttccactg gtggtgggca taatagggct gctctagcta ttaaagaaga acttacttcc
      361 aaaacacttg atggagagcc aattgaatgt gaaataatag atagtttgaa attagttaat
      421 aatacaatgg ataaaataat ttcaagagga tacgaaaaat ctgctatata tactcccaaa
      481 gcctatggaa gtgtatatag attatctgaa acaaatcttt tatcaaaaaa tgaatttaaa
      541 gataatctac taataacatt catggctaaa aaatttaaaa aacttattag aagcgaaaaa
      601 ccagacttga ttataggaac gcatcctttt ccaatgattg ctctaagtac attgaaaaaa
      661 aactttaatt tacataataa tgaatcaaat gcttacacag agcattttta taaacattat
      721 acaaatacca ttaatgttcc tccgcttatt tctgtattaa ccgattatac tactcattct
      781 acatggatac aaaatgaaat tgattactat attgttggtc atgaatatgt aaaagaatta
      841 ttggtgtttg atggagttga accgagtaaa attagaacat ttggaatccc tgttgaaaaa
      901 tcttttctct ctcacagaga taaaggtatt gttctttctg agttgaattt atctcctgat
      961 aaattaactg ttttacttat ggggggtagc tttggggctg gaaatataaa agagacactt
     1021 gatgaattac tggatactga tagagacttt caaatacttg taattactgg taagaatgaa
     1081 agcctaaaag aaaaaatcga aaaaaaactt atgtctcgtt atcatgataa aaatgtatgt
     1141 gttcttggat acactgataa gatgaatgat attttagctt ctatagatgt attagtaagc
     1201 aaacctggtg gacttactac aactgaggct cttttaaaag atgtacctat gatagttcct
     1261 tattatatac caggtcagga agaagagaat cttgatttct tatctaattg tggtgcagca
     1321 cttagaacta caaaaaaata taacttgtca atattactaa aagttttaat agatgaccct
     1381 agcagattag aaatgctaaa aagaaatatt aaatcaataa gaaagtcaaa ttcagcccaa
     1441 aatatagcta atttaatttt aaatataatt tctggttgat a
//
LOCUS       DAELTA010000305         1453 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.310, whole genome shotgun sequence.
ACCESSION   DAELTA010000305 DAELTA010000000
VERSION     DAELTA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.310"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            253..705
                     /locus_tag="KPG13_003631"
     CDS             253..705
                     /locus_tag="KPG13_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG0987291.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(862..>1453)
                     /locus_tag="KPG13_003632"
     CDS             complement(862..>1453)
                     /locus_tag="KPG13_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG0987292.1"
                     /translation="NYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYNKEAFNESLR
                     NSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKKGCQMGYRIV
                     LLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLDKYFSDRKQL
                     SIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT          481 a          221 c          140 g          611 t
ORIGIN      
        1 accacattta aaatatttat taatttaata taaatatttt ccgttattat taaaagtagc
       61 tatatttcgt taatattgta tcactatcgt gtagcaataa caataagtac attttatata
      121 ttttttataa tattatttaa tcatccaaac ttttaccact ttttcaatat ataaaatatt
      181 acactttaga tgtaacaaaa tcgcatccat ttaaatattg tattttatag actaatttta
      241 ggatgtgata ctttggttaa tttaaatgca aataatctaa atgtaaatat ggtttatcct
      301 atttcttcca cagcacccat acgtttcaga agatatactg tttcatattt agaaaacatg
      361 gaaaatattt atttaactat agcaacatac tttgatacac ttggtttagg cacaatgaat
      421 attgacaaag tttatggtca atgggcttgg tttactgata atatatatga tttaaatcta
      481 tttgtttttg taggaaatta tccttatcaa attgctaaaa acagatatga tacatttgta
      541 gatattttgc cagtagctat tacttctatt gtcaatggag atagagtatt tttaatttca
      601 aatccttgtt tgttaaattc aaaaattaca gttagattta tatctagtca tcctactctt
      661 aatagaacag ttgattatgg caatattaga gattttattt tttaaaactg caacatgatg
      721 tgctattaaa aaatacccta aatttaatga tattattaaa taactagaat atttttaatt
      781 ggcacatcat gcctagctat tataaattca taatattatt ttacttaaga taagtactgt
      841 attttatcta tttgtatatt tttatttacc tagtttctca gttctatatc ctatttcttt
      901 attagctagc ttataacctt cttgtatcat ttcttctctc gttaatgcac tccctaataa
      961 attgtcaata ctcagttgct ttctatcact aaaatactta tccaataatg attcctctat
     1021 atcttcctga tatgccaaat ttccttcatg cattatatat tttacatcaa aaccttcttt
     1081 atttaatact tttcccacca agatacttct atgacatctt ataggttctt gcatagctcc
     1141 taataaaact atcctataac ccatttgaca accctttttt aatctttcta ttccgtttaa
     1201 aaaaatatct tcttttgcta ctttctcaaa atctgcataa ccttcttgcg tatatacatc
     1261 tttattagtt cttttagctc caaattcttt tcccatgtat atatataaaa atccggaatt
     1321 tctaagagat tcgttaaatg cctctttatt gtattgaata ttatatttag aatatggagt
     1381 ttctctaatg tcaactacac aatttatgtc atattttttt atcatattaa taagcttatc
     1441 atatggataa ttt
//
LOCUS       DAELTA010000306         1412 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.311, whole genome shotgun sequence.
ACCESSION   DAELTA010000306 DAELTA010000000
VERSION     DAELTA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.311"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            120..365
                     /locus_tag="KPG13_003633"
     CDS             120..365
                     /locus_tag="KPG13_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG0987293.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(583..852)
                     /locus_tag="KPG13_003634"
     CDS             complement(583..852)
                     /locus_tag="KPG13_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG0987294.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          569 a          213 c          157 g          473 t
ORIGIN      
        1 atgaaagagc ataaataaat tgatataata aaaaattaaa tattaaatat taactataat
       61 aaaaaaatat ctggtaatag gtataatata aaaagaatat accagaaaga aggtgaagta
      121 tgggagaact acaaaaaatt ccaggggtag ggaaagcaac agaaaaatct ttaatcatgt
      181 taggatatac aactattaaa tcacttaaag atgcaaaccc agcacaaatg tatgaaaagg
      241 aatgtttaat gcgtggacag cacatagaca gatgtcagtt atatgtatac agatgtgcag
      301 tatattttgc atcaactgaa aatccagaac cagaaaaatt aaagtggtgg tattggaaag
      361 attaaaaaat caaaaaagtt agacataaaa aagagttgaa tatccgtttt aaataataaa
      421 aactaatact caactctttt ttagtcgtaa attttatgta aatctatttg tatggctaaa
      481 tatacccatc aataaatgta taaatatact aatcaacaaa tgactaaaca tactacctaa
      541 caaaggatta aataaattcc ttaacaaagc attctaaaac aattaattag aaactgtttc
      601 ttcaagccat tttctacatt gctcaatatt taccataggg tcaaaacctt ggtcttttcc
      661 cttatttaaa agcatatcac aagggaaagt atcacattca ccacaatgat taagttcctt
      721 actttcacag caagttttaa ccttacattc tccaccccaa aatggttttt ccatagttgg
      781 gcaaccagtg caatttactt gttctttttt tgtacaactg tcacaacaga caccacatct
      841 tgattcaaac atattcaaac atcctttcca ttatttaaaa atattataaa atgattttag
      901 atgaatacaa aagtgcaaat agttgatttt aggtcatcta aatgttaaac ataaaattat
      961 tagtactggt aaatttataa aaactattcc aaaaatcttt ttgaatattt caaaacacaa
     1021 taaattactt caaatcccct tgaatatatt ttacagcatt atagattgct ttaaatctct
     1081 ttgaatattc tacaagacta tagattagtt taaatccctt tgaatatatt ttacaacatt
     1141 atagattgtt ttaaatctct ttgaatattc tacaagacta tagattagtt taaatccctt
     1201 tgaatatttc aaaacaccat agattacttt aaatcccttt gaatgttcta taacactata
     1261 aattactttt aacttttata taaccataaa gtttttatct ggcactttaa atggagtttt
     1321 atatcaaatt tgccttccat cgaataacca aacttaattt aattacacat tccaagagtc
     1381 aaatattcat tcaatatatt tgataattct at
//
LOCUS       DAELTA010000307         1412 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.312, whole genome shotgun sequence.
ACCESSION   DAELTA010000307 DAELTA010000000
VERSION     DAELTA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.312"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            189..1286
                     /gene="ychF"
                     /locus_tag="KPG13_003635"
     CDS             189..1286
                     /gene="ychF"
                     /locus_tag="KPG13_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG0987295.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          561 a          149 c          246 g          456 t
ORIGIN      
        1 atttttattt aaattaaata caaatattat ttaaatcaat atgctttaaa gtctagttta
       61 caagcttgat atatatgatg tataatcaat atgtatgtct ataattaaaa caattttgca
      121 atcctataga ttgattgttt aaagttatat aaagaatata aaaaagaata agagagagga
      181 cgattaatat gaaattaggt atagttggtt taccaaatgt aggtaaaagc acactattta
      241 acgcaataac tcaagcaggt gcagaatctg caaattaccc tttttgtaca atagacccaa
      301 atgtgggagt tgtatctgtt ccagatgaaa gattaaacaa actacaagaa ttatataatt
      361 ctgaaaaaat agttcctact gctatagaat tttgtgatat agcaggttta gttagaggag
      421 cttctaaagg agaaggttta ggaaataaat tcttatcaca tataagagaa gttgatgcaa
      481 tagttcatgt tgttagatgt tttgaggatg aaaatgttgt acatgtagat ggttctgtag
      541 acccacttcg tgatatagaa actataaact tagaattaat tttctcagat atagagattc
      601 ttgaaagaag aattaataaa actcaaaaag ctgctaaggc tgataaaact ttaggttctg
      661 aacttgattt attaaaatca ataatgtcta ctttagaaga aagtaaatgt gtaagaacta
      721 tggaatttac agaagatgaa caaacattcg taaattcatt ggatttactt acatctaagc
      781 ctgtaatata tgcttcaaat gtatcagaag atgatttagc tgataatgga gaaaataata
      841 aatatgttca acaagttaaa gcatttgcag aaacagaaga tgccgaagtt gttgtagtat
      901 gtgcacaaat agaagcagaa atttctgaac ttgattctgc tgaagaaaag aaagagtttt
      961 tagaaacatt aggtcttgaa caatcagggc ttgataagct tataaaatct tcttatgctt
     1021 tacttggttt aatttcattc ttaactgctg gtccaaaaga agttagagca tggactataa
     1081 aagttggaag taaagctcct caggcaggag gaaaaataca ttctgatata gaaagaggat
     1141 ttatcagagc agaaacaata gcctttaatg atttggttga gcatggtact atggctgctg
     1201 ctaaagaaaa agggttggtt aaattagaag gtaaagaata catagttaaa gatggagatg
     1261 taatattatt tagatttaat gtttaaaatg attaatcgta tacactttga ctattatata
     1321 taataactaa aaaactaaga ttataataat acaatattat cttataataa ttatatagaa
     1381 tttaaaaata aaagaagagt atttcaaatg at
//
LOCUS       DAELTA010000308         1348 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.313, whole genome shotgun sequence.
ACCESSION   DAELTA010000308 DAELTA010000000
VERSION     DAELTA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.313"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(204..>1348)
                     /locus_tag="KPG13_003636"
     CDS             complement(204..>1348)
                     /locus_tag="KPG13_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG0987296.1"
                     /translation="IGISSKVKEMESNGVKVINLSIGEPDFNVPNNAKSYGIDSLNKD
                     YTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSITNTLLALTDEGDEV
                     LLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSITDKTKILVINNPSNP
                     TGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIASLSEEAKDITITING
                     FSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIAYGALKDCSIDIDNM
                     VKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFEYKDSFSIEFCNQFL
                     EEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKIMA"
BASE COUNT          443 a          196 c          146 g          563 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttca tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa ccatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcat ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattg
//
LOCUS       DAELTA010000309         1337 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.314, whole genome shotgun sequence.
ACCESSION   DAELTA010000309 DAELTA010000000
VERSION     DAELTA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.314"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            27..101
                     /locus_tag="KPG13_003637"
     tRNA            27..101
                     /locus_tag="KPG13_003637"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KPG13_003638"
     tRNA            116..200
                     /locus_tag="KPG13_003638"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KPG13_003639"
     tRNA            210..293
                     /locus_tag="KPG13_003639"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="KPG13_003640"
     tRNA            323..399
                     /locus_tag="KPG13_003640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="KPG13_003641"
     tRNA            407..482
                     /locus_tag="KPG13_003641"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="KPG13_003642"
     tRNA            571..659
                     /locus_tag="KPG13_003642"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="KPG13_003643"
     tRNA            663..738
                     /locus_tag="KPG13_003643"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="KPG13_003644"
     tRNA            745..818
                     /locus_tag="KPG13_003644"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="KPG13_003645"
     tRNA            833..909
                     /locus_tag="KPG13_003645"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="KPG13_003646"
     tRNA            933..1009
                     /locus_tag="KPG13_003646"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="KPG13_003647"
     tRNA            1017..1093
                     /locus_tag="KPG13_003647"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="KPG13_003648"
     tRNA            1102..1177
                     /locus_tag="KPG13_003648"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="KPG13_003649"
     tRNA            1185..1258
                     /locus_tag="KPG13_003649"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="KPG13_003650"
     tRNA            1265..1336
                     /locus_tag="KPG13_003650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAELTA010000310         1263 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.315, whole genome shotgun sequence.
ACCESSION   DAELTA010000310 DAELTA010000000
VERSION     DAELTA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.315"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            197..907
                     /gene="rgaR"
                     /locus_tag="KPG13_003651"
     CDS             197..907
                     /gene="rgaR"
                     /locus_tag="KPG13_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG0987297.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          500 a          102 c          201 g          460 t
ORIGIN      
        1 tattttttta cataatatat ctagaaaagt ataggaattt atcgtaattt aagtttaaat
       61 attaaagaga atttctataa gtagtttaat cgacaattta aatttataaa gaaatttaaa
      121 tttgaaagct agtgagtaca gtttggaaat tgaaataaag taaactaaat gatattttag
      181 atttggaggt gatattatgt tcagggtagt tatatgtgat gacgaaaaaa tacaaagaag
      241 catactaaaa gagtttacag aaaaactttt gtctgaaaga tgtctaaatt atcaaatact
      301 ggaattttcc tgtggagaag atttaatatc caaataccca gaaaaaattg atattatatt
      361 tttagatatc caaatgaaag atatcaatgg aattgaggtg gctaaaaaaa taagaaagtt
      421 tgatgaaaaa gtagaaatta tatttacaac ggctttttca gagtatgccc cccaaggtta
      481 tgaggttcgt gcttatagat atttagtaaa gcctatagag tatagtaact ttgcaatggg
      541 tgtaaatctt tgtattgata accttcaaag aaagaaggag cgttatatag ttttaaatag
      601 taaaaaagga tttaatagaa tactgataga ttctatttta tatgttgaaa ctgtaaagag
      661 agatttagtc gtacatacaa taggtagaga ttataaagct aatatgagca tgaaacaagc
      721 agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat acaagtttta ttgttaattt
      781 aagaagaata gaagatctta gggataatat gattactatt aatgaacaat ttatacctat
      841 aagtaagtat agacttaaag atttaaaagt agcattaaca agcttgttat gtgatataat
      901 gtattaattt taaaaaggta attgaaaata tcgtaaaaaa gatttaatgt catcacaggg
      961 ctattgattg aaactgtctt aatatataaa atgttattta tattaagaca gttttattat
     1021 gcaaaatagc agtttataga tttttaatat ggtttaacat gtatatgtag aggttccatt
     1081 atagtagcat gattattaat tagtagcttg attatcaata tactattatt gatttttata
     1141 tgcattatat gtatttttag aatatgatta cctttttaaa ataatgatta tttataaaac
     1201 tctatagata tttgtttcat ttagctacgc atactttaat taattatatt aatatttaat
     1261 gga
//
LOCUS       DAELTA010000311         1261 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.316, whole genome shotgun sequence.
ACCESSION   DAELTA010000311 DAELTA010000000
VERSION     DAELTA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.316"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>1261
                     /locus_tag="KPG13_003652"
     CDS             <1..>1261
                     /locus_tag="KPG13_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987298.1"
                     /translation="KNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNN
                     EKVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKS
                     TSNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHK
                     WFNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLI
                     NGKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVC
                     GIITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKIT
                     EKTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSRE
                     FALFRGIGISKHEINKIVKLESYIYIIV"
BASE COUNT          574 a          120 c          179 g          388 t
ORIGIN      
        1 aaaaaccaga actatataaa cacatacata atttctatat tattaagttt aggaactata
       61 ataatatcag tttcaaaacc tcttatgtta tcagttaaaa ctgatccaat gatttgtata
      121 agaagaaata atgaaaaagt ggatataaaa caaaattcct ttataaataa atttatgata
      181 aaacttttca aagactatgg taacatagca tcaaaaaata tacaaagaaa taagaaacgt
      241 acaaatttat ctatagcatc aatggtaatt atatttttct taatggcaac aatatacaca
      301 aagtctacta gtaacttttt aagtgatggt gtattaaaat attggataca aggagattat
      361 ttattacata atattgatat aagtactata cgttcaaata ataaaggtta tgataaaaat
      421 gtactacaag agataaaggc aattgatggt gtaacaaaag taaactcata taggcataag
      481 tggtttaata taaaaattga tgataaaagt ataaataaga actctgatta ttggaaaaaa
      541 aataaagata atattgaagc tagatcagaa gtaaaagatg gaattaaggt atataataat
      601 tcttttgaat ttttaggaat agaagataca gatatactag aggatttctt gataaatgga
      661 aaggaaaata tagataaatt tagtaaacaa ccatatctgt atataacaaa gaaatccagt
      721 gattcgctta atataaaaaa aggagataga ataaaagtat actttgatat tatagattca
      781 aaaacaaata attttaccaa aacaatatct caagaattta tagtatgtgg aattataact
      841 acattaccag taacctcaca agtgggagct gaatttggcg gagtaatatc aacaaatcaa
      901 tttaataagt ttacaggaat atcatcttat gaaagatttg acatatggac gagtaaacta
      961 gccaatgata aatatgttga aagtgagtta aacaaaataa cagaaaaaac aaataaagga
     1021 attttaattc catacaaagc tgaaacagct gaatatgaaa aaattgataa tcaaaaaaca
     1081 ctaattatgg tattagtaac tggaattata gttatattat ctttatttaa ttgttgcaat
     1141 actatagtta ctagtattaa tagcagaagt agggaatttg cactatttag aggaatagga
     1201 atatcaaaac atgaaataaa taaaattgtt aaattggaaa gttatatata tattatagta
     1261 a
//
LOCUS       DAELTA010000312         1259 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.317, whole genome shotgun sequence.
ACCESSION   DAELTA010000312 DAELTA010000000
VERSION     DAELTA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.317"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            426..800
                     /locus_tag="KPG13_003653"
     CDS             426..800
                     /locus_tag="KPG13_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0987299.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            905..1259
                     /locus_tag="KPG13_003654"
                     /pseudo
     CDS             905..1259
                     /locus_tag="KPG13_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          570 a          116 c          169 g          404 t
ORIGIN      
        1 aataattata tgaactatca gtataataag tcaaaatatg tatacatgaa tacatatttt
       61 aaaagatata tattattaca tacctaaaaa actttcataa aaacaaaaat aaaatatgct
      121 tttagtaact tgttaacata acttataatt tattttataa gcattagcct atatagcttt
      181 gtgctactaa atctctttga ttctaacgat ttagtgctat tctacaacta aaataataca
      241 atagagctat ttattgtttg gtagcattgt ttgtaaatta aacaaattta tacatatgaa
      301 tattatttta cattcaaaaa aacagactaa atatgtagaa tatttggtaa aacttacctc
      361 tagtttttgt cttaaaaagc tcctattatt aacttaagtt agaagaatta aggaagtgac
      421 ataaattgaa agaagaaata ttaatagaaa aattattaag agcagaatta atagtaatgg
      481 agtatttatg gaaaaaagac actctaatgc ctaaaaaaga aattgtaaaa gaaataaaac
      541 agatttatgg atggcataaa agtacaataa aaatactact aaaaaggcta gttgctaaaa
      601 ggtatttggc tagatatatt ataaattctc aatctcatta taaaataata gatagtaaag
      661 aatattatac ttttaagaaa aaggtactaa aatctagtaa aagcataaaa ataatgcgtt
      721 cacttaccac tacacataaa aatgtaagta aagagaagtt agacctatta gatgagtatt
      781 atagaaactt aaaagaatga tatagtaaaa atcgaatgta attatttacc ttaattcttt
      841 tgtttgtaaa actttattga tacagaattt aacatataca taaattggta aaggaatgta
      901 tactatgaaa attagtaaat taccagaagc ggaattaaaa gttatgagat atatttggga
      961 atcagaaaag gtattaatat caagagaaat caccaagtct atggaacaaa aatatgaatg
     1021 gaaagataca actatattta cagtattaaa aagattacta aagaagagag tttttagaag
     1081 cggagaaaat agataagtgt acacactata atgtattagt aaaaaaaaat aatatcagag
     1141 attttaaaca aaagaatttt taaagaatac acataaaaat tcaatattaa gtttaatgtc
     1201 agcactatgt agtaaggatg atgaagtaga tggtgatatt ttaaatttat tagaagaat
//
LOCUS       DAELTA010000313         1257 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.318, whole genome shotgun sequence.
ACCESSION   DAELTA010000313 DAELTA010000000
VERSION     DAELTA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.318"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..461)
                     /locus_tag="KPG13_003655"
     CDS             complement(<1..461)
                     /locus_tag="KPG13_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987300.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQT"
     gene            complement(448..1215)
                     /locus_tag="KPG13_003656"
     CDS             complement(448..1215)
                     /locus_tag="KPG13_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987301.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT          426 a          226 c          117 g          488 t
ORIGIN      
        1 gtctgaacta aggcctttat acttaagttc atatttacta ctatcatttc attcattaat
       61 tttacaagca tcatagtcat aagctttgaa aataacagtc ctaaaaatat acctattagt
      121 attgcaacta tacccatcaa aaaagtttca tataaaaata tttttcctat ctgatttttc
      181 ctcattccaa gtaaactata agttcctact tctcgcctac gtctattgaa aaaaaatgag
      241 tttgaataat atataaataa aaatacaaat accactatta caattgaact tgtattaata
      301 ctcatcctag ttttctctcc aagtgaactc aacgcttcat tatactctat agatttaaaa
      361 ctaaaaaata caaatacact aaatacaatt gaaataaagt acattaaata attaaaaaaa
      421 ctattcttta cattttgcat tgcaattcta aataaagtca tttttattaa ctcctccaat
      481 taaggaaact gtatttacta ttttattata gaactctctg ttactttctc cctttacaat
      541 ttctgtgaat attattccat cctttataaa gagtattcta ttgcaaaaac tcgcacttat
      601 agcatcatga gttaccatca atattgtttt attgctgtct ttgtttaatt ttaatagaca
      661 attcaacaaa tccctagcag atttagaatc taatgctcct gtaggctcat ctgcaagtac
      721 taatgatggt tttgttgcta tagctctaca tacagctcct ctttgctttt gtccaccaga
      781 aacttcgcca gggtatttat ctaatataga ttctatattt aattcttttg aaatctcttt
      841 tattctattt tctattactg atactctttc attttttaaa gaaagaggca acattatatt
      901 ttcttttatt gtaagtgtat ccaaaagatt agaatcttga aatacaaagc ctagattatc
      961 acgtctaaaa tctgccaatt cattttgctt cattttaata gtattctttc catttatata
     1021 aatatctcct gatgaaggta aatctattgt agaaataata tttaaaagtg ttgatttacc
     1081 agcaccggaa gaccccataa taccaacaaa ttctccttta taaaccttga aagttattcc
     1141 tcttagagct tcatgttttg aaccttttct tccatactcc ttttttatat tttcaacaga
     1201 taatatttct tccatagtta ataatcacct ttcatcacaa ttagttattt atcaatt
//
LOCUS       DAELTA010000314         1256 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.319, whole genome shotgun sequence.
ACCESSION   DAELTA010000314 DAELTA010000000
VERSION     DAELTA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.319"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            63..1175
                     /locus_tag="KPG13_003657"
     CDS             63..1175
                     /locus_tag="KPG13_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG0987302.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT          537 a          127 c          172 g          420 t
ORIGIN      
        1 aattattttg tgatatatat atgttataat aaatgtataa aattttgatt ggtgggttat
       61 atatgaatgc atttattaga aaaagaaata aaaactatgt tgtttacctg gaatttgaag
      121 atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac ttttgataaa aaaagggaag
      181 caagtaaaag actaaatgag gtaaaagaaa gcttatataa tgatggtttt ttagtaccaa
      241 gcgaaattac agtgtcagaa tttctattag attttctaaa taaatatagt gaaaatatat
      301 ctttagctac atataataat tatgttagta tttgtaagaa ttacattaat ccatctattg
      361 gaaaatataa aatacaagac ttacatccgg ttcacataca aaactacata gataaactat
      421 cttataaact aaatcctcaa tcaattaaga ttcatataaa tatattgaaa cttgcaataa
      481 aaagagctta tagactcaaa ttaataaaag aaaatgttat ggatagtata gaggctccaa
      541 gatataaaaa atttaaaaat gagatttatg atagagaaca aatgataaaa ttattagatt
      601 tagcaaaaaa tacagaaatg gaattaccta ttaatttggc tattggatta ggtcttagaa
      661 tttcagaagt tttaggtcta acttgggata atattgattt tgaagaaaat acaataacag
      721 taaataagat aacttcccga attaatggtt ctgttattct caaagagcca aagacagaaa
      781 gctctgttag aaaaatatct tctcccaaag aattaatgtc tttattaaaa gaatataaaa
      841 taaaacagaa taaaaattta ttaaaatctt ccattagaaa taataataat ttgttatttt
      901 tcaataagaa atgtgaacct attgctgaag atgttatgag taaaaaattt aaaagatttt
      961 tagaaaagaa tgaattacct catattagat tccatgattt aagacactca catgttactt
     1021 tacttataaa ttctaaagta cctataaagg ttatatccga gagagtagga cactcaaata
     1081 taagtactac tctaagcgtt tattctcatg tacttaaaga aatggataaa gaggcttctg
     1141 acaaaatttc tgaaagttta tttaatgcta attaatatta atttatctta gctttaaatt
     1201 agcttagctt attgagatag attcattaat attaaatgta tctatctcaa attaat
//
LOCUS       DAELTA010000315         1251 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.320, whole genome shotgun sequence.
ACCESSION   DAELTA010000315 DAELTA010000000
VERSION     DAELTA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.320"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            98..1162
                     /locus_tag="KPG13_003658"
     CDS             98..1162
                     /locus_tag="KPG13_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG0987303.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT          472 a          132 c          222 g          425 t
ORIGIN      
        1 acgcttaaat tgaaaatata actttattct tatataatta aattatacaa caataaagtt
       61 atatttttta aatatatttt tactaggggg aatagaattg aaaagggttg atgagagaaa
      121 tactatgttt gcaagagcaa attacaaaaa agatagtact gcttataatg actattataa
      181 gaaaaatcct gacaaaaaag aaattgatga tagtataaga aatagaccaa atttatgtag
      241 tgaaggaaca atgacttaca atgagttaaa ctctccaatg gcaagtagtg cattcgattt
      301 tttatctgat atcaaatctt tatgcgaagg aaaagtttct gatactaaag tagatgtaga
      361 tgcaaagtca atgacaaaaa aaattaaggg tcttgcaaag cagtatggag cttctgtagt
      421 tggtataact aaattaaaag actatcatat ttatacaaat aggggtagac atgaagaaaa
      481 ttacggtgaa gaaattaatc taactcataa gtatgcaatt gtatttggat gtgagatgga
      541 taaagaaatg ataaatagag ctccaatgat atgtgaagtt atagaaacat caaagtgcta
      601 tgtggatgca tctatcgtcg gcatgatttt aagttactat ataagaaatt taggatatga
      661 tgctagaaat catatggatg ctaattattt agtaatgcct gtttttatag ctcgtgatgc
      721 tggtttagga gatataggaa gaaatgctat acttacaaat aaagattatg gctctagact
      781 tagattaggt gttgttacta cagatattcc tcttttagaa gatgaatatg ttgattttgg
      841 actagaagat ttttgtaagg tctgtaaaaa atgctctttt aactgccctt ctcattcgct
      901 ttctaatgat attaaaattg gtgatgatgg aaaatataat tgggtaattg aacatgagac
      961 atgctatatt aaatggagat atttaggtac agattgtggt atgtgtatat ctgcttgccc
     1021 atttagccaa aatctcgaaa ctataaagaa tactacttca tttaagggta ataatgagct
     1081 tattcaaaaa gctttagatg agtatacttc aaaatttggt aaaagaatat ttatacctgg
     1141 aaatccatct tggttgaaat aattttattt aataaagaat ctgtactaga atttatcata
     1201 ggacaggttc tttattaaat atataaattt ttaaatactt tttctcgttt a
//
LOCUS       DAELTA010000316         1189 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.321, whole genome shotgun sequence.
ACCESSION   DAELTA010000316 DAELTA010000000
VERSION     DAELTA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.321"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(304..795)
                     /locus_tag="KPG13_003659"
     CDS             complement(304..795)
                     /locus_tag="KPG13_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG0987304.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          461 a          166 c          110 g          452 t
ORIGIN      
        1 atttttatat ataatgaaaa atgtgataag tatccaaaag aattactgga tataatagga
       61 tagggtattt agccttttaa gataaataaa aatatctaca actatatatt gcaaaaattt
      121 aaaactaatt tatatatgct aatataaata tttataaaga cataagacaa atttacctac
      181 agatttaaat ttaacttata tattttaaaa ggctaatata atatgtattt tcttctatac
      241 aatcaaaagg cttcatatct aagatatgtg atgacactaa tattcacaat tttttagtaa
      301 ttatcacatt ataaaaaata ttacagctag tccatcatca gatgcttctt gtataaactt
      361 tttaagctca attagatttg cccatacata ttcaaaaaaa tcatcttcac tctcatcatt
      421 cataacagga taaatattat tttctaacat ctcattaata ttaaatcttt ttctaaatgc
      481 ttgttcttcc aaactgttca ttgcttttga tagttttttt acatcatttg ctgtaatcaa
      541 catgacagga aattctccat caatttccat aagtatgttt ccacttagta ctaatttgct
      601 aaatatatta tcatcatctc caccaaaagg acaacctgtc aaagtaaagt gaatggcatg
      661 ccatgcttta tcaatattga tagtactatt ttcgttggca tcttcaaaaa caaaatcttg
      721 taagttttct tctgcttgct gtaatttaaa aacattctct tcacttattt tagtataaca
      781 tcctatcatc cccataatta cattcctcct aagtagcgtt tttttaattg taacttataa
      841 ttaaacttag ttacaataaa atatattgag aaaagtatta tttcatctta aatttaaata
      901 cctatattat caactaaaat tctgtgttta tagcgtaagt attattattt tttcctaaaa
      961 attaaatgaa tcagaagttc tataggcaat attgactata aataagaact attactttta
     1021 aaaaaaaatt taagaaatta tactgtagaa tcatgctcta cttattagct ttaaataaaa
     1081 aataaatcat gttctcaaat taaataataa ataacaataa atttaaattt taaactataa
     1141 gagtttctga agtgttaata agcataccaa taattatatg tgtaagaat
//
LOCUS       DAELTA010000317         1182 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.322, whole genome shotgun sequence.
ACCESSION   DAELTA010000317 DAELTA010000000
VERSION     DAELTA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.322"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(312..818)
                     /locus_tag="KPG13_003660"
     CDS             complement(312..818)
                     /locus_tag="KPG13_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG0987305.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          425 a          169 c          105 g          483 t
ORIGIN      
        1 actttgaaat tatttacatt aaaataaatc aaatacaaat gccataaatt aagtgtttct
       61 agttagcata aaaatactta taaagtatac tcaaatattt caaataaata cttttattca
      121 aataaatctc tacttatata agtgcttata taaataaaaa gatgaactat agtaaatata
      181 gttcatcttt tttataatag tatttattta aaattaatat ttatttagtt actagcatct
      241 acttagatat cagataagaa agcacagtca tttacttata ataagttctt ttcatatatt
      301 ttttattata attattcttt tacaagaact tttataattt ctgaaatgta catagtatgg
      361 tagtctttct ctggatacca ttttttatca ttttctttag catcaaagtt ttctggtggc
      421 atattaccat gatatagctt tttacatact ataatcatat tagcttcttc aaaagctact
      481 gtatcatcca caaaatatgg tgtaagtcct gctttttcta ttttatttat atctctacca
      541 gaaatagtac cacaaatatt taaagcctct ctatacgttt catcaaagaa ggcaactgta
      601 aatgtatcat tactatctac aaattctttt gtatatctct gtggtcttat gtaagttgta
      661 acaacatttt taccccaata gactccaaca ccaccccagc tagcagtcat tgtgttaaac
      721 ttttctgaat caccagctgt tataagtaac cattctttgc caatcttagt aaatggatta
      781 aactgtaatt cctcaatttt tacttctcta aaacccatga cttcatccta cccttcattt
      841 tttaattata taaattaatt gttaaaaatt tgaatatatg tactcttata atactgactt
      901 ttgattgtcc tacataattc atgtaaatta taatacaaca aatcaattac tttcgcaata
      961 agtagttttc atattttata agtgtaaaat atagataaaa tatattgatt ataaagtata
     1021 taatatattt ttaaacctaa agcaatatac aataaaaaaa tgtagaaagt aagtatctct
     1081 ctacattttt tattgtatat tatttttata ttaatttaat tttatcttga ttcagtttta
     1141 taaaaaattg ttataattgt caagttctac tctattattt at
//
LOCUS       DAELTA010000318         1176 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.323, whole genome shotgun sequence.
ACCESSION   DAELTA010000318 DAELTA010000000
VERSION     DAELTA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.323"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            576..>1176
                     /locus_tag="KPG13_003661"
     CDS             576..>1176
                     /locus_tag="KPG13_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG0987306.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIED"
BASE COUNT          473 a           97 c          174 g          432 t
ORIGIN      
        1 tattaaatta aggactataa tttgaaaata agctataaaa actcaatatt gttttgttca
       61 aagataaaaa aagaatactt taggtgattt ttattaaaaa ttaagagtga taagattata
      121 tactttataa atgtaaatat gacggataaa aatgataaat ataataatat atatatttac
      181 attaggagtt ttctatacta taattattta aaactaaata taaatgttgg aattatttag
      241 gacttgaaat tgtctaaaaa taaatataag tgatgaagag gtgatgtgat gagattagat
      301 atggatagta tgttatatgt actaatttat atgacattat tggtatctat cattggaagt
      361 atattttatt tttgtaaaga cctcttaaat cataaaatta agtatgtttt aataagagac
      421 atatttgtaa atagaattac atattctgaa gaacggttgc ctaaacagta tatagttttt
      481 cagaaatatg atatttggag gtattgtagt ttgttttaat ttgaaatgaa tctattataa
      541 attctaaagt ttaaaaataa aaaggagaga ataaaatgga tttcatttca agtatattgc
      601 aaggattatt aagtatatta tttaatttta caggtgattt tggaatttct attgtactta
      661 taactttaat agttaaatta gtacttatgc cactatcatt aaaacaaaga ttttctgtaa
      721 aaaaacagca agaattagct gaaaaaatgg aacatataaa agaaaagtat aagaatgata
      781 ctaaagaatt ggaaaagcaa atacaagtaa gttctgtaga aagtatgaag agtatgctgg
      841 gatgtcttat agttttggtt caaatgccag tagtatatgc tttatatcat gtatgtttag
      901 atatgacaaa ggaatttaca acaattataa ttccatggat tgctaattta ggtacatttg
      961 ataatttgtt tatattacct tgtatatata cattaattat gttagcttca aatttagtga
     1021 attatatacc atatttaaaa ataaattctc aagtaaagtt taacaaacaa atggctgtgg
     1081 tgactaccat aacaagtctt atactaactg ttagaactcc agtagccata ggtctgtact
     1141 ttataacaag tgcaatttat tcattaattg aagata
//
LOCUS       DAELTA010000319         1176 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.324, whole genome shotgun sequence.
ACCESSION   DAELTA010000319 DAELTA010000000
VERSION     DAELTA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.324"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..1101
                     /locus_tag="KPG13_003662"
     CDS             61..1101
                     /locus_tag="KPG13_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG0987307.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          461 a          140 c          194 g          381 t
ORIGIN      
        1 tcagaacatt tgtatttatg ttataatttg tataaatata tttatttagg aggaaacaat
       61 atgaatttaa atttaactaa tactataaat ttaatgaaaa aatatttgga cattccaagc
      121 ccagctggat atactgaaaa tgcagtttta gaaatcaaaa aagattttga aaatttaagt
      181 cttgacacta ttcttacaaa aaaaggtgca cttatagcta ctcttcctgg tgaagatgat
      241 tcaaatcaaa taacaatatc tgcacatatg gatacacttg gagctatagt aaaagaaata
      301 tcatctgatg gtactcttaa ataccataaa gttggtggtg gatgttgggc ggctatagag
      361 ggagaaaact gtactgttat aactagaaaa ggtaaaaaaa tcagaggaag tgttgtattt
      421 aagtatgctt ctactcatat atatggtcaa acaaaagctg ccactgaaag aaatgaagaa
      481 acaatgatta ttagacttga tgaaaaagta tttacaaagc aagatgtatt agatttagga
      541 ataagtgttg gggattttgt atgcctagac catagatttg aggctacaga atctggtttt
      601 ataaaatcta gatatataga taataaatca gctgtagcaa tggttcttga aatatgtaga
      661 tattttaaag aaaacaattt aacacctaag catactacta acttttatat aagtaattat
      721 gaagaaattg gacatggtgt ttctaaatgt ttacctgaaa aaacaaaaga atttgtagca
      781 atagatatag cacctactgg accaagtcaa acatctagtg aacatggtgt aactatagtt
      841 gcaaaagata atttatcatt atatgatttt aatttaagaa acaagcttgt cgatgttgca
      901 gaagacaata atttagacta cgctgtcgat gtatttactt tctatggttc agatgcttca
      961 gaagctattc gttggggaga agatgttcaa atagcatgtg ttggtcctgg aacaaacaat
     1021 agtcaccatt atgaaagaac acatatagaa gctattgaaa atacactaaa attacttata
     1081 aattatatgc ttaaagaata attaattata tattttacca aagtcaattc ttaacaaata
     1141 attaacttta atttttattt ttatacaaaa attggt
//
LOCUS       DAELTA010000320         1156 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.325, whole genome shotgun sequence.
ACCESSION   DAELTA010000320 DAELTA010000000
VERSION     DAELTA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.325"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(119..688)
                     /locus_tag="KPG13_003663"
     CDS             complement(119..688)
                     /locus_tag="KPG13_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG0987308.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            891..1118
                     /locus_tag="KPG13_003664"
     CDS             891..1118
                     /locus_tag="KPG13_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG0987309.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
BASE COUNT          384 a          167 c          156 g          449 t
ORIGIN      
        1 aaaatactct ataaaagcta aaaagttttt ctattcgcta catctaaaaa taattagata
       61 gaggtataaa tgtatttagt ttatttatac ctctagtctt ttataaatta tatctctact
      121 aaattgattt tttagcacga ataagtgtgc tcttggagat accagtcatc ttcacaacct
      181 ctctatatgg cttagaattt aataacccca atgcatattg tatctgttga ttgctgtatt
      241 tctttggcct accatcttta aagtcctttt tctttttagc aaattctttt cctaattgag
      301 tacgttctat tatcatattt ctctcaagtt ctgcaactgc aagtaatgta gtcaaaaaga
      361 atttacccat tgaagtattt tctaaaaggc ctatatttaa aatatgaaca cttacttctt
      421 tagcaaataa accttctatt agttgtattc cttctgatgt acttctagct agtctatcca
      481 gcttagttgc aacaagtaag tctccctttt ttagagatga tataattttt tcaaatttag
      541 gcctgtttgt agtagtgcct gtataagttt cctttattat tttagcatca ttatatttat
      601 tcaaaatttc ttgctgttgc tgatacaaac tgttgttgtc tgtttgattt tttgtactta
      661 cacggcaata tccatatata ataatcatca cttcctaaca taaagatttt gtacagaagt
      721 tacccagaag cttgttttta cttatcttta tccattttac taaaacgatt tttgggtgtc
      781 aatactgtta acttttgaca ccataataat tcctttataa tttgtttgac tatgtcaaaa
      841 attaccaata taatgaaatt aatgagttta tacaaattgg aggaaataat atgaatagtc
      901 tgttaaaaga agcaatagat gaattaaaaa acatccattg cggggaagtt tttttattaa
      961 aagaattgtt caaaggctat gaatggaaca ggttggagat gaatatcaga ttgaaattag
     1021 gaaaactttt tttgtatgaa gtggtaacta atcccaattt aaatgtagag gtcatcaata
     1081 aaaattctgc ttatcagcaa atgtataaaa aaaaatagtg acttaaatgt atataatagt
     1141 ataatcttct tattga
//
LOCUS       DAELTA010000321         1155 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.326, whole genome shotgun sequence.
ACCESSION   DAELTA010000321 DAELTA010000000
VERSION     DAELTA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.326"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="KPG13_003665"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="KPG13_003665"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            163..237
                     /locus_tag="KPG13_003666"
     tRNA            163..237
                     /locus_tag="KPG13_003666"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Asn,seq:gtt)
     gene            243..328
                     /locus_tag="KPG13_003667"
     tRNA            243..328
                     /locus_tag="KPG13_003667"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:277..279,aa:Leu,seq:taa)
     gene            344..419
                     /locus_tag="KPG13_003668"
     tRNA            344..419
                     /locus_tag="KPG13_003668"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Met,seq:cat)
     gene            427..501
                     /locus_tag="KPG13_003669"
     tRNA            427..501
                     /locus_tag="KPG13_003669"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Glu,seq:ttc)
     gene            516..589
                     /locus_tag="KPG13_003670"
     tRNA            516..589
                     /locus_tag="KPG13_003670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Gly,seq:tcc)
     gene            595..670
                     /locus_tag="KPG13_003671"
     tRNA            595..670
                     /locus_tag="KPG13_003671"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:628..630,aa:Val,seq:tac)
     gene            676..752
                     /locus_tag="KPG13_003672"
     tRNA            676..752
                     /locus_tag="KPG13_003672"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:710..712,aa:Asp,seq:gtc)
     gene            763..837
                     /locus_tag="KPG13_003673"
     tRNA            763..837
                     /locus_tag="KPG13_003673"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:795..797,aa:Gly,seq:gcc)
     gene            847..920
                     /locus_tag="KPG13_003674"
     tRNA            847..920
                     /locus_tag="KPG13_003674"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Arg,seq:tct)
BASE COUNT          325 a          252 c          279 g          299 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgttaaat gttccaaggt agctcaacgg
      181 tggagcaacc ggctgttaac cggtaggctg tgggttcgag ccccaccctt ggagccaaaa
      241 aagccgaagt ggcggaactg gcagacgcac aggacttaaa atcctgcggg acttacctct
      301 cgtaccggtt cgattccggt cttcggcact tataaaacaa tatcgcgggg tggagcagtt
      361 ggcagctcgt cgggctcata acccgaaggt cgtaggttca agtcctgcct ccgcaaccat
      421 taaaacggct cgttggtcaa gaggttaaga caccgccctt tcacggcggt aacaggggtt
      481 caattcccct acgagtcacc aattcaatta aatatgcggg tgtagctcaa tggtagagtt
      541 ctggccttcc aagccagctg tgagggttcg atccccttca cccgctccaa aaatgggacc
      601 atagctcagc tgggagagca cctgccttac aagcaggggg tcacaggttc gagccctgtt
      661 ggtcccacca ttcgcggcct ggtagttcag ttggttagaa tgccagcctg tcacgctgga
      721 ggtcgagggt tcgagcccct tccaggtcgc caaattaaat aagcgggaat agttcagtgg
      781 tagagcgcaa ccttgccaag gttgaagtcg cgagttcgaa tctcgtttcc tgctccataa
      841 taacttgggt gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg
      901 ttcgaatccc tgtgcgctca ttaagaaaag tttactataa attatagtaa acttttatat
      961 tgtttaaaaa gacacaggaa acatgtatat tacctgtgtc tttttattta taaactttaa
     1021 actatgtaaa aattacagta tattctaata gagttacagt atattcatta atattgtaaa
     1081 catacggagg gtaatgtata atagtctaca agatgtttat gaaggcatca gttaaatata
     1141 ataaatataa ctaat
//
LOCUS       DAELTA010000322         1151 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.327, whole genome shotgun sequence.
ACCESSION   DAELTA010000322 DAELTA010000000
VERSION     DAELTA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.327"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(202..912)
                     /locus_tag="KPG13_003675"
     CDS             complement(202..912)
                     /locus_tag="KPG13_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0987310.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          413 a          152 c          125 g          461 t
ORIGIN      
        1 ttgaaattct agaaactgag ctttaaaaaa ttaagtttga aaaaaactct aaaaactaag
       61 cttcaaaaaa ttaaattttg aaagattgaa attctagaaa ttaagcttca aaaaattaaa
      121 tttgaaaaaa caaaactcta aaaactaagc tttaaaaagt taagtttcaa aaataaaatt
      181 ctagagattg aacttcaaaa attaagattc taacatatac cttgtaaatt ttcctacaac
      241 ctcttgatat tttttacggc ttataggaat tttttcattc tgaactttgg cataagtttg
      301 agttatacta tctacatggt ttagattgac aatatacctt tgatgcgtac gtataaaatt
      361 agagggaaga acagtttcca tatcatttaa ttttccgtag tattctaatg ttttatcaaa
      421 ggtaactaca gttaattttc ttagctcact tttaaagtag aggatatctt ttgtattaat
      481 tttacaaaga ttagttccaa tctgaactgt aataaagttt tctttccatt gttctaattt
      541 ttttagtgct tcttttaata ctttacatat tttttcagtt ttataaggtt ttaagatgta
      601 gttcaaagct cctacatcat atccatgaaa tacatagtca gaaaaacctg tgacaaagat
      661 gattatagta tcttttgaat gttttcgtat agtttttgct atgtcaacgc cattactttt
      721 tccaagttca atatctaaaa aaataatatc tatgtaataa ttttcgtgtt ctagattgta
      781 tactagactt tctccatttg aatattcttt tatttggtag gggtagcccc acaaatccat
      841 ttctcttgta atgattttat tcagcatgtt tctttgttgt ttttcatcat cacaaataca
      901 aatcgaagtc aattttttat ctcctttcaa gttttatatt tatatagtta agtatataat
      961 atattttgct acctagatat ataaaatgaa ataacaatta tatccagata gcaaaataaa
     1021 attttttata ttagtatact tacacttaaa actattctat taaacttatt ctgaagtgct
     1081 aatgattttc ttattaaaaa atctaacaat ctttcttatt ataaaataca aatgctttta
     1141 ttattataaa a
//
LOCUS       DAELTA010000323         1097 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.328, whole genome shotgun sequence.
ACCESSION   DAELTA010000323 DAELTA010000000
VERSION     DAELTA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.328"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            163..915
                     /gene="cwlD"
                     /locus_tag="KPG13_003676"
     CDS             163..915
                     /gene="cwlD"
                     /locus_tag="KPG13_003676"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG0987311.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          488 a          110 c          165 g          334 t
ORIGIN      
        1 tataatataa actaagtata atttattgta tagtattgaa tgttagataa acatagtgaa
       61 ttaaataggg acagaaatat aatatcatta cataataata tattaatacc aataaataaa
      121 gatatatcaa tattaaaaaa atttaaatgt ttgtactaat atatggaata ttatagtgtt
      181 tttataagta tacatataca gggtgatgtt gtgagaaagt acataaaaca tataattttt
      241 agttttgcaa tgatatgtct agtagtagta tccatatttg aaattaaaaa tatttctgaa
      301 gatgttatca agtatatgcc agtaacaaac aaaactataa ttttagatgc tggtcatgga
      361 ggcattgatc caggtgcatt aaataaggat aagagtacat ctgaaaagga tattaaccta
      421 gcaataacac ttaagcttag agagcttata gaatcaagtg gtggccttgt aatattaact
      481 cgagaagatg acagtagcct ttataaagaa gaaaataata aaactacaag acaaaaatat
      541 aatgagaatt tgaaaaatag aaaagaaata atatctaatt ctaatgccaa tatgtttgtc
      601 tctatacatc ttaatgcatt tgaacaatca aaatactatg gagctcaaac tttttatcct
      661 aaagacaaac aagacagtaa agaattatcg aagtgtattc aagaagaact taaaagagta
      721 gtagataaga caaacaacag agaagtcaaa ccaagggatg atatatatct tttgaaagaa
      781 aataatattc catcagtact gatagaatgt ggttttttat caaatgaaaa agagtgtaaa
      841 ctcttaactg atgaaacata tcaagaaaaa atagcatggg caatctacat aggaatacaa
      901 aaatatttaa gttgaaataa ctataaaaat aggtctaaat atgtaaatag cttgtattaa
      961 agcagttaaa atgcattaat ataggctatt tttattttga caaaaaaata tttaaaataa
     1021 aagttaaaaa gttgttgact tagaataata tagatgatat tatatatgag tgcccaaaag
     1081 gagcaccaaa ataagac
//
LOCUS       DAELTA010000324         1094 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.329, whole genome shotgun sequence.
ACCESSION   DAELTA010000324 DAELTA010000000
VERSION     DAELTA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.329"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            368..1018
                     /locus_tag="KPG13_003677"
     CDS             368..1018
                     /locus_tag="KPG13_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG0987312.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          466 a          101 c          182 g          345 t
ORIGIN      
        1 aatgaattaa tctatggtat aatagtttaa tttgaagtac aatgaattaa tctatagtat
       61 aatagtttaa tttgaagtac aacgatttaa tctatagtat aatagtttaa tttgaagtac
      121 aatggtttaa tctatagtat aatagtttaa tttgaagtat aacgatttaa tctatagtac
      181 aataacttga tttgcaatac aatgaattaa tctatagtat aagagcatga tttacagtac
      241 aataatttaa attgtagtac aaaagtacta tcaaataaga atttctactt taaaaatatt
      301 actaaaagta attaaagtcc agtaattcaa tataatatgg caaaagaata gaaataaggt
      361 gagagtagtg aaagtaaagt caaatttaga tgaaatttta tatcataaga ttattgaaag
      421 tcttattcgt ggtgaatact ctgtaggtca aaaaatatta ctaaatgacc tttgtgaaaa
      481 atttgaagta agcagaacac cagttgtaca agcagtaaaa atgctaaata aagatggagt
      541 gcttactatt atgacaaatg gtaaagtgta cgtaccagag tatgaatatg atatggtaaa
      601 acaagtttgt gaaacgagaa cactgattga aacatatgct ttagaaaaaa tgatgcaaga
      661 agaggaagag gtttttcaac aaaagctaga tgtaataaaa agatactctg ataaatgcga
      721 ggagtactat cgtcaagaaa aatctgtaga acttgcacta gcagatttgg aattacataa
      781 agccattgta gaaggggcaa ataatgagat tttaaatgat gtatatgttg gtatacaagg
      841 aagatttata gtagtgaact atttaattcg accattaaaa aatcgtaatt atgaaggaac
      901 agtacaagac cattttgaaa tattaaaatt cattgaagac agagatacaa aaaaagcagt
      961 agataaacta agaaatcata ttcaaggaac tattaaaaga ttctgtgaag atgaataata
     1021 agttttgaag ttactatata tacttatcta ttaagttgct aaatgtagtc taagtataat
     1081 ctaattatag ttta
//
LOCUS       DAELTA010000325         1082 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.330, whole genome shotgun sequence.
ACCESSION   DAELTA010000325 DAELTA010000000
VERSION     DAELTA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.330"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(180..926)
                     /gene="cdtR"
                     /locus_tag="KPG13_003678"
     CDS             complement(180..926)
                     /gene="cdtR"
                     /locus_tag="KPG13_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG0987313.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          398 a          133 c          111 g          440 t
ORIGIN      
        1 tatcgaatca taatctagga acaattgtta taatatctaa tagtagttat atatgatata
       61 gtcagtatgt gttttttcac aatatgatta ttatatagag gattcttagg gattactctt
      121 tttgtataaa aaagagatgg ttcaaatttt tatcttgaga catctccttt ttctatttat
      181 tatgttttaa taatattctt taaaatattt ctatatgtat tagatatacg acatatatgg
      241 ccattactca ttttaatatt atttttcttt aggtcgagag aaacaatatg atatttatta
      301 actacaaaag atttatgtac acggataaag catgcatcta aatctggtaa agactttaaa
      361 cttttataga attcaaatat tctagaagta gtcactaatt ttattttatg tggaatagtg
      421 cttgtttcaa aatatataac ttcatcaaat gaaattttcc atagtgagaa atctgaatag
      481 atagtaaaaa aattagagcg ataatttttt tctttaatgt atcttttgtg agcaatttta
      541 atacaagctt ttattttact ttcagcaatt tccatatcat gttttagtat aaagtccata
      601 acttctaacc tataatcgaa gatccctaaa atcatttcta tatgatttgt aagaaatatg
      661 attggactaa tataatctga ttctctaatt ttttgtgcta tccataatcc atcacataat
      721 tcattactta tcttatattt tgcgtctaca actataaaat atattcttac tttatttttt
      781 tcagtaagct cttttaataa caaatttgca ttatgatata atcttacaac atcattatca
      841 aaaccttctt ttattaaaat attatttatt ttttctttta gtttcattcc aaaacaaaca
      901 tcattatcaa agattaatat atccactaaa taccctccta taaaaaattc aaaagtaaaa
      961 aaacaaaagt attttataga aaatacataa aataacacta ggagataaat aagattaagg
     1021 atagttagaa ttaaggtata tttatgcttg tttatatagt atatcttata cttatagttt
     1081 ac
//
LOCUS       DAELTA010000326         1071 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.331, whole genome shotgun sequence.
ACCESSION   DAELTA010000326 DAELTA010000000
VERSION     DAELTA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.331"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(202..1071)
                     /locus_tag="KPG13_003679"
     CDS             complement(202..1071)
                     /locus_tag="KPG13_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG0987314.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          400 a          167 c          104 g          400 t
ORIGIN      
        1 atcaaaaaat aaactaaaaa atatcgaaaa ataatttaaa agacatcaaa actaaatctt
       61 ctaatcagca attaaaagtc tattttctta gtttaaaaaa taaagataga ttttttcaaa
      121 acactggata aataggtaaa aaaataaaca gttagtaaaa taaattttta ctaactgttt
      181 atttttattg tttaatatgt cctataaatc ctctaagtaa tattttcctt gcaaaaattc
      241 tgattctaaa cctagatttt taaaataatc aaccatgccc atacacgcaa atttttctgt
      301 agaataatga gtagcaccta taacattaat taaattatct tttgctattt gatggaactc
      361 taatactggt tcaaaatgtt ttaaaggctt agtaaaccca gtaatatata gatttatacc
      421 aagttcagca acttctcttg ctacaaatgg ataacttcct ccccctgctg caatagcaac
      481 taaaccatct tttaatacag aatctccata atcatacaat ttcacatcat gaccaacaga
      541 ttttttaatt aattccttaa tctcttctat tgattttagt gatgttttac atataactcc
      601 tacctcaatt ttatcgtcat atttacaaaa tggtttgact atttctaaac ccatagcttt
      661 tgcaaaacta acagaagttg aatagtcact atagttgtct agtggagaat gtaatacata
      721 aaaagaaatt ctacgatttt tcatctcttc catataagat agtggtatgt cgtgaaatgg
      781 aaaaccttca tctgatgcta tgtatcccat tgcatgatgt gaaaaaatta aaatatcttt
      841 ttcatcttta tctataactt gcttaattat ttctttatca ggaaaagtag ttgtgtatac
      901 tttattaata ttttgagcat tatctaatac taatcctatg tatttttttc taaattctgg
      961 agtaataaaa tcattttcaa aattcataaa actccaatca tcattaatat ttttaatatt
     1021 aaattctaaa tctaattttt tataaagttc aattgcctta ataataacca c
//
LOCUS       DAELTA010000327          976 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.332, whole genome shotgun sequence.
ACCESSION   DAELTA010000327 DAELTA010000000
VERSION     DAELTA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.332"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(137..820)
                     /locus_tag="KPG13_003680"
     CDS             complement(137..820)
                     /locus_tag="KPG13_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG0987315.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          383 a          151 c           77 g          365 t
ORIGIN      
        1 ttactatact taaaatactt ctattaactt atacattgct tactatattt taaattcttt
       61 aattaatcta tacaatattt gctatatata aagtatatca aataacagct tcattttttt
      121 gtaaatttac agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc
      181 caaagaccac tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg
      241 taaaaaaagt tcctaaaata gctccagata taaagaatat aataatagca taatactgta
      301 aactcttttt tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag
      361 cacttcttaa atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa
      421 aactctctac ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc
      481 ttcctctagg aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct
      541 gacgccaatg aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa
      601 atacacctaa cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac
      661 cttctgctat attaagccca aataaaacta tatttccagt ttgtgcatta gcaaagactt
      721 tacctcttac cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac
      781 ataaaaagaa tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct
      841 aatgatagat acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt
      901 atttatattt aaatattgtc tatatttata cattatttgt atttatatag cattcatatt
      961 tatttatatt taaaca
//
LOCUS       DAELTA010000328          967 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.333, whole genome shotgun sequence.
ACCESSION   DAELTA010000328 DAELTA010000000
VERSION     DAELTA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.333"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            35..>967
                     /locus_tag="KPG13_003681"
     CDS             35..>967
                     /locus_tag="KPG13_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0987316.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFN"
BASE COUNT          383 a           86 c          175 g          323 t
ORIGIN      
        1 aatataatat aggaaaattg tatggtaaat aaatatgaaa aatagtttta aaaattgtat
       61 tagtttaaga aataaattta taattatatc aattgtgttt ctattaatcc tattaattgt
      121 atcttatata tttggcaaat tagtgtataa tagttcagtt ggtttagagc agttaataaa
      181 aaacgaagat atagtaaaaa tattttctga gagaaaagat agaccactgg agaaattaaa
      241 aaactacaag acatctgaat taatgataca aagttctaat aattacaagc ttgaaagtct
      301 ttttataact tcaaatataa agacaagaga tactatgata cttgttcatg gaattggaag
      361 tagttactat gaaatgttaa aggtagcata tagatattta gataaaggtt ataacgtatt
      421 agtttataat caaagaaata ctggtaatag tggtggagat aattacacat ttggtcttta
      481 tgaaaggtat gatttagata gtctagttaa atttgtaaaa aataaatttc ctgaaggaag
      541 attaggagtg catggatttt ccatgggggc agggacagct gctatgcatt cagagataaa
      601 ttcaaaagat gataaggtcg atttttatat actggattct ccttattctg aaatgaaaga
      661 tgcaattaga atgggagtac tagaaaaacg aataccagat atattaatca attatgtggt
      721 gacttgtgga gatttatata ataagtttaa atctggattt tggtacagtg atgtaaaacc
      781 atatgagtca gttgagaaat ctaatgtacc catactgttt atacatggaa caaaagatac
      841 tgtttgtaat tatcaaaata gcaagaaaat gtatgattta gttaaacatg ataaaaaaga
      901 cctatggctt atagaaggta ttggtcatgt tgatggattt gaacatgata gtacagtata
      961 cttcaat
//
LOCUS       DAELTA010000329          947 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.334, whole genome shotgun sequence.
ACCESSION   DAELTA010000329 DAELTA010000000
VERSION     DAELTA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.334"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            270..737
                     /locus_tag="KPG13_003682"
     CDS             270..737
                     /locus_tag="KPG13_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBG0987317.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          365 a           62 c          159 g          361 t
ORIGIN      
        1 taaaaaaaca tagatatttc agtaatctaa aatagtctac aaaaataaca gttttagcaa
       61 aattaataag aatacatata gatttaaacc taaaagtatg aacatgtctg ttttttacaa
      121 gattaaaatt tatattatat gataaaatta acttaactat ttcaataaga cttataattt
      181 ttttctttat actagacctt attgaaacct ttatagttta attctaatta tatattataa
      241 ataaagtatg tgtatattaa ggaggtaata tgaagttttt aaatgtttta gtagtagttg
      301 aagatattga gaaatcaaaa aagttttatt atgatgtttt ggggttaaag gtaatctgtg
      361 attttggaga aaatgttgta ttagaaggta atatatcatt acaagaaaaa aagctttggt
      421 tagaatttat taataagagt gacagtgaag ttaagttcaa tggaaatgat gctgagttat
      481 attttgagga agataatttt gatacttttg ttgaaagatt gagtacaatg aaagatatag
      541 attatgttca tctagctata gagcataggt ggggtcaaag agcaattaga ttttatgatt
      601 tagatgggca tattattgag gttggagaaa ctatgtcatc tgtgtgtaga cgttttttag
      661 atagtggtct ttcaatagat gaggtagcta aaagaatgga tgtaactgtc gaatatattg
      721 aatcagtttt agaatagatg gattatatta aagtaaaatt gaggtgaagg ctgtaagtaa
      781 acttgtgtga aaacaaattt gcttacagtt tttatttttc taaaataaag aatataatac
      841 ttgtttagct ttatttgagt ttttaaaaat attatgatat ttcttatata tttatactaa
      901 agtttaaatt tagaaaaaat atataaaaga tagttatatt tactatt
//
LOCUS       DAELTA010000330          941 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.335, whole genome shotgun sequence.
ACCESSION   DAELTA010000330 DAELTA010000000
VERSION     DAELTA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.335"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..840
                     /locus_tag="KPG13_003683"
     CDS             <1..840
                     /locus_tag="KPG13_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG0987318.1"
                     /translation="LNILSIVSIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQ
                     SKQYSVKELTKLYKFLVIKANETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDI
                     LPGVSDSYSKPKYIISSNLMCYTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQ
                     RGFASEDEANFIAYLTSIKHPNIDFNYSGYILALNYTASALSKVDYNAYVDISAGISD
                     SVRRDLKNESEFWQKYEGKINEISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELY
                     PYN"
BASE COUNT          350 a          124 c          129 g          338 t
ORIGIN      
        1 ttaaatatat tatccattgt atcaatagtc tactttcttt ttgtagtgct ttggggaata
       61 aactataata ggattccact agaaaccaca cttataaata actataattt aaaaaacaat
      121 acctctatcc aatcaaaaca atatagtgtt aaagagctta ctaaattata taaattcctt
      181 gtaattaaag caaatgaaac tagaaaactc acattacaag ataaaaatgg tattgtaaaa
      241 tctaatacag attataatgg tgtcataaat agagctcaac taggatacga taatatcttg
      301 gatatattac ctggtgttag tgatagttat tctaagccta agtatattat ttcatcaaat
      361 ctaatgtgct atacaggaat aactggtata tactttcctt ttacaggtga ggcaaatgta
      421 aatatagcaa tacctgactt atatattcct tgtacagttg aacatgaaat ggcacatcaa
      481 aggggatttg ctagtgaaga tgaagctaat tttatagcgt atcttacttc tataaaacac
      541 cccaatattg attttaacta ttctggatat attttagctt taaattatac tgcttctgct
      601 ttaagtaagg ttgattataa tgcatatgtt gatatttctg ctggcatttc agattctgtc
      661 cgtagagatt taaaaaatga aagtgaattt tggcaaaaat atgaaggtaa aattaatgaa
      721 atttctaatg agtttaacaa ttcttactta aaagctaatg gcgttactga aggtactcag
      781 agttatggta aaatggttga tttattgcta acttattatg aattatatcc atataactaa
      841 acactacaat tcaaaatttt attcatatac agctgatatt aaatcagctg ttttctattt
      901 aaataaatta tttaacttca agcaatctta tataatttga t
//
LOCUS       DAELTA010000331          854 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.336, whole genome shotgun sequence.
ACCESSION   DAELTA010000331 DAELTA010000000
VERSION     DAELTA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.336"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..427)
                     /locus_tag="KPG13_003684"
     CDS             complement(<1..427)
                     /locus_tag="KPG13_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987319.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLF"
BASE COUNT          347 a          130 c          108 g          269 t
ORIGIN      
        1 ggaataaatc taaaaggact ttacagatga atacaaaagc taaggctaaa actataggag
       61 atatttcccc attgaataag tgtttaaagc taaatagagg aatatttgat tttcccatta
      121 ttcttaaatt gatagagtat agcattccca taactaaaat accagaaagt aaattagata
      181 tcttaaactt aatgtgaaga atccctgtaa caagaccagc agtacaacca cataagatag
      241 ccattaaagt tgctactaca ggagaaatac cattagtaag agaaaatgct acaatagaag
      301 cacccattgt atagctacca tcagcagaca tatctggaaa atctaagatt ttgtaagtta
      361 tataaacccc cagtgccata atcgataaaa ttaaactttg cgtcattaca gatataatac
      421 ctgacataaa taaaacaccc ctttgaaaat attttaaatg gtttttaaaa tttggtttta
      481 aaaaatgatt ttgatataaa aaaaccacac agtatatata actgtatggc aaaaattcga
      541 ataagccaca cagtataatc catgtggctc tatattatga tgataaaata aaaactttaa
      601 caacaattac tatagccaac atagatacaa tccttaaaaa tcataatcct aaggggcttg
      661 tatctatgtt atgacaattt gtcttaactt aggtacaaac cctaactaaa ttggctacta
      721 aaaaatcgta cttgtttgtt taataaagtc ttcatattta cctctccttt taatttaata
      781 attttgaaaa ttttgttttc tgttttgtat agtaattagt ttaatacagt aaaaaataaa
      841 agtcaactat aaat
//
LOCUS       DAELTA010000332          852 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.337, whole genome shotgun sequence.
ACCESSION   DAELTA010000332 DAELTA010000000
VERSION     DAELTA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.337"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            89..466
                     /locus_tag="KPG13_003685"
     CDS             89..466
                     /locus_tag="KPG13_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG0987320.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          336 a           64 c          131 g          321 t
ORIGIN      
        1 tgagatattt taaataaaac ctttgactcg aaaagtctat taatatagta tttaattatg
       61 tagaagtttg agaggagtgt aaaatcatat gactattact aagataccac cagcagaatt
      121 aaaagtcatg aaatttattt ggagttcaga ttttacagta gcatcaaaag atgtcattga
      181 agctatggaa aaactatata attggaagca aactacaaca ctaacacttt tatcaagatt
      241 agtaaataag caattcttaa gtgcaagaaa aattgataga catacacatt atacagttct
      301 tattggagaa gaagagtatt tacattttga aacaaaaact tttttagata atatgcatgg
      361 aagttctatt gagagtttta tgaaatcctt gttcaaaaag ggggtaaatg aagaagaatt
      421 gaactctgtt gaaaaatggg ttaaggatat taaaaaagat gaataatttg gtgtagttaa
      481 ttatcataaa ataaatagtt tttacaataa ttagttaaat gaatcaaata taaagtttta
      541 tgtaaaattt gtgattttta taaatatttt tgtcttgtta tttaatatta atacaataag
      601 tttgatgtaa aatcaaacct gtttgaattt aatttatatt gggtttgtca ttaatattat
      661 tttagtttga tattgtatac gaatttaaaa gtcagaattt ttgtatatta atcatgtaaa
      721 ttaattgttt tagatataaa gggcataatt ttatttttgt tttgattatt tctagggtaa
      781 gataagtata caatagattg tattaaacat gcttatattg atagatttga gataaatatt
      841 gagatatatt aa
//
LOCUS       DAELTA010000333          847 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.338, whole genome shotgun sequence.
ACCESSION   DAELTA010000333 DAELTA010000000
VERSION     DAELTA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.338"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(159..>847)
                     /locus_tag="KPG13_003686"
     CDS             complement(159..>847)
                     /locus_tag="KPG13_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG0987321.1"
                     /translation="NEDFERSKYFSYDVKYPVIYNIRNQSTYVNPVILNNINNAVRMT
                     VNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTFNKNYVLSMILNLMGFANNY
                     GPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDYIKSQINENREMYYSNVELV
                     IPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSDFGNFINLRFYCISPDIYVQ
                     SRGRAKHFMG"
BASE COUNT          302 a          129 c           96 g          320 t
ORIGIN      
        1 acaaaacaat cattctattt taacatttat caatatgtct aaatttatat aattaacaca
       61 tacgaatagt attgtttaaa tcacattatt taagatagat aaatcttaac cctactccag
      121 cataaatact aaaatagggc ttagaattat ctatattttt atcccataaa atgcttagct
      181 cttcctcttg attgaacata tatatctggg gaaatacaat agaagcgtaa atttataaaa
      241 tttccaaagt cagaaaactt cagttcaaac ttaggtatac caaaactttc aggtgctatt
      301 tcgtcaacgc caaaatagat gataattcca ttatctgtaa gataaaatgc ttggtcttca
      361 ggtataacta actcaacatt agaataatac atttctctat tttcatttat ttgagattta
      421 atgtaatcag taattatatt aatataatca actccatctc taaaaatgtc attaagtaaa
      481 agttcttttc cagttgtcaa atctatattg taattattca atgtattgta tttaggccca
      541 tagttattag caaaccccat aaggtttaat atcatactca gtacataatt tttattaaaa
      601 gtaactgcgt aactggtaga tacccaatag ctttgttttg gaagtgaatt ttgagaagct
      661 gcgttattat attctcgttc ttcttcctgt aagccatttt taaaattatt tacagtcatt
      721 cttacagcat tgtttatgtt atttaaaata actgggttta catacgtgga ttgatttcta
      781 atattgtata taacaggata ctttacatca tatgaaaaat acttacttct ttcaaaatct
      841 tcattaa
//
LOCUS       DAELTA010000334          844 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.339, whole genome shotgun sequence.
ACCESSION   DAELTA010000334 DAELTA010000000
VERSION     DAELTA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.339"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            80..778
                     /locus_tag="KPG13_003687"
     CDS             80..778
                     /locus_tag="KPG13_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG0987322.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          319 a          108 c          130 g          287 t
ORIGIN      
        1 taaattctat attacaacat tattttatag tttgattaat ttaacaatca ctaatatatc
       61 aaactgaaag gagattttta tggaaaaaaa atatttcgaa attcatggag ttccttcaat
      121 cctttgggga aatccttcaa gtaaattata tatttacata catggacaat gtggcaataa
      181 agaggaggct gaagcttttg ctaatattgc agttccacat gagtgtcaag ttttaagtgt
      241 tgatttgcct gaacatggag aacgtaaaag agaaaaaaat tcatttaacc catggcatgt
      301 tgttccagaa ttagtatcta ttatggaata tgcaaagtgc cactggaatc aaatttcgtt
      361 atatgcaaat agcattggag catggtttag tatgttaagc tttgaaaaag agaacttaag
      421 agagtgtatt tttgtatctc caattctaga tatgcaacag ctcatttcaa atatgatgct
      481 ttgggcaaat gtatcagaag aacagcttaa acatgaacta attatcccaa cttcatttgg
      541 gcatactctt tcatgggaat atctacaata tgtaaaacaa catcctatca ctcaatggaa
      601 tgtaccaaca aaaatattat atggtaaaca tgatgaactt actgaaatcg gtgttgtaga
      661 gaaatttgta gaaaagttta attgcaacct tactattata gaaaatggca aacattggtt
      721 taacacacca caagaattaa attcattata tgaatggtta aatactttag ttaaataaaa
      781 aataattata ttattaatat gattaaatta tttataaatg ttatttaatt aattttattt
      841 tagc
//
LOCUS       DAELTA010000335          834 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.340, whole genome shotgun sequence.
ACCESSION   DAELTA010000335 DAELTA010000000
VERSION     DAELTA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.340"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            195..>834
                     /locus_tag="KPG13_003688"
     CDS             195..>834
                     /locus_tag="KPG13_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG0987323.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEE"
BASE COUNT          360 a           77 c          138 g          259 t
ORIGIN      
        1 acaaaataaa aaagtaatta tagaatttat caatatatta aaaaactgaa ttgtagtaaa
       61 ctaagtgcaa ctaaaattac aggagtatta aaagagtatg atgattttat agtaatgaaa
      121 gttaatgatg atgatattat tttaatataa gaagggaaga aaaaatattt agacaaaaac
      181 taagaggtga gcttatggag tattttagtg agtcaaataa tgaagtacaa aaatttgatt
      241 ggggagaggt tatgtggata cacgaaccat caaaaacaca attcaataga cttagtgctg
      301 gaattgtaag attttttccg ggaaactacc aagaaaagca ttttcattta agtgaagaac
      361 agcttttata tgtgattcag ggagaaggaa tacaaataat tgatggtaaa aaggtcaata
      421 taaaggaaac atctatagtt tattgcccac catattcaga acatgaaatt ataaacactg
      481 gtaaaattga tttagtaata ttaattactt atgttcctca taaattttcg agtttgagac
      541 aaatacctat agtattttct gaaaacaata tacaggagtt agttaatgta aacataattg
      601 aaaatcttgc aaatcaaata tctaatatat tgaagcttag aatatctgta tatgacttaa
      661 agtataaaga aatatttgag actaagaaag aaaataaatt ttgcgatatt tgtaaaaata
      721 taaaacagtg tactaaaaaa cttgttaaaa gcataaataa tgataacttt gaaaaagtgt
      781 accattgtga gtatggtgtt tccagtttag agatacctat agtattagaa gaaa
//
LOCUS       DAELTA010000336          828 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.341, whole genome shotgun sequence.
ACCESSION   DAELTA010000336 DAELTA010000000
VERSION     DAELTA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.341"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            14..>828
                     /locus_tag="KPG13_003689"
     CDS             14..>828
                     /locus_tag="KPG13_003689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0987324.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKN"
BASE COUNT          344 a           51 c          117 g          316 t
ORIGIN      
        1 tgggagatgt attatgttaa atataaatgt attttggatt tttttaccta tattttctac
       61 ggcgatagaa tggattgtat taaaattttt attaaatgaa tttagtgagt taagaaagaa
      121 tagattaacc ctaaatagta gcatagtaat tgctattatg ataattacta tgttgaatac
      181 aattgagttt aatataaata ttaaattatt tatatgtata tttattacat atgtattgta
      241 taaaataaat tatgtagtgg ataaatggaa aagtgtattt ataagtttgc tatattggat
      301 gttattaatt ggatttgata gtattggatt aagtatagta atgacactta attctataaa
      361 aaatatgaat gatttattag aggataattt gttgaagtta gagttaataa tgatatctaa
      421 atcattacta atagtattaa taccattgct taagtttatc agattaaaaa caaaaataaa
      481 caagagggat tgtatatatt tatctattcc attaatagca aacattattg gtgtaatagt
      541 aatatttgaa tttattttta aagataagat tatcgattct aaagagagtt taataatact
      601 tacaatatca actgtatttt tactatcaaa tctttcatta gtaagcataa taggaagaat
      661 aataaaagat aatagtttaa agatagaata tgaaattaca aaaaaaagaa tgaatatgca
      721 atataaatat tatttaaatt tacaggaata tcagttgaaa ataagaaaac tatatcatga
      781 tatgaataat catatcattt gtattcagaa tgtttatggg aaaaatga
//
LOCUS       DAELTA010000337          824 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.342, whole genome shotgun sequence.
ACCESSION   DAELTA010000337 DAELTA010000000
VERSION     DAELTA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.342"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>824
                     /locus_tag="KPG13_003690"
     CDS             <1..>824
                     /locus_tag="KPG13_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG0987325.1"
                     /translation="KVFEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELAN
                     IAKKTIEKDLKIEINEDEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLI
                     KAKLENIFKEKLEILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKE
                     IRLIEKYIETGNIYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDM
                     KKSYFEREKIATTEIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIML"
BASE COUNT          350 a           71 c          142 g          261 t
ORIGIN      
        1 ataaggtatt tgaagagttt ttgtataagc atattagtag tttatgtaaa aaaataaagt
       61 taggtataaa tcaatcacct ataatggctc aagatataaa aataaaatat ccatttgctt
      121 ttgaactagc taatatagct aaaaagacaa tagaaaaaga tttgaagata gaaattaatg
      181 aagatgaagt tgcaaacata gctctacata taggaggagc agttgaaaga gcatcttata
      241 ataataaaga taaggttttc aaaactataa tagtttgtac ttcaggcata ggcacatcaa
      301 tgttaataaa agctaaatta gagaatatat ttaaggaaaa attagaaata ctaaagatta
      361 ttccttcata cttagttgaa tatataaaag ttatagatgt tgattttgta atatctactg
      421 ttcctattga agtaggagat gtgccagtaa taaatatatc tccaatttta agtgagaaag
      481 aaattagatt aattgaaaaa tatatagaaa caggaaatat atacctagat ttagatataa
      541 aaagcctgtt tgatagtgaa ttgtttttta ctgatttggt gtttaaaagg aaagaagaag
      601 ttatagattt tatggcaagt aaacttgtac aaaagggcta tatagatgag gatatgaaaa
      661 aatcttactt tgagagagaa aaaatagcaa caacagaaat agggaatatg gtaactatac
      721 cacatggagc tattggaaaa atttttgata ataaaatagc aattggtata ttaaagaagc
      781 ctatttcttg ggaaatatca gatgttcgtt taataataat gtta
//
LOCUS       DAELTA010000338          818 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.343, whole genome shotgun sequence.
ACCESSION   DAELTA010000338 DAELTA010000000
VERSION     DAELTA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.343"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(62..760)
                     /gene="yunB"
                     /locus_tag="KPG13_003691"
     CDS             complement(62..760)
                     /gene="yunB"
                     /locus_tag="KPG13_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG0987326.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT          242 a          123 c           94 g          359 t
ORIGIN      
        1 ttccaaaaat tttatttata aactattatt ttgaatttat ttaaaaatat acaactttac
       61 attacttgtt ggtattcttt ccagtgttag gtaaaacatt tcctagattt ttttctggaa
      121 tatttacata actttctgga acatcaccta ctattatagt ttcacaaata ggtatttgag
      181 ctttaatttg ttttgttgaa gttataagtg gtattactac tttaacttgt gtttgagctt
      241 ctaaatatat tctatgtcta gtttgattta tacctgaaga ctcaaattcc gttttaaagt
      301 ttactgatac tgttcctata ggttcaatag atacttcaag tttaggaccg tatttagcta
      361 atataggact tcctaatgct gttcctatag gtatgtaaga acttgctgtc ttatcctttt
      421 ttaattcatc ttgtatttct aaagctattg cagatgcaat ttcattcatc attatagtgt
      481 ttgcttgtat catagttatt ttaccattat tatcaagttg tatatccatt aaatcttcat
      541 aatttatttt tccttctacc atctcagcaa cagctttatt tatcgaccta tttgctagtt
      601 caagagcttt agtctctgct aaaactgtta ttgtcggtct taaagtttta tctatatata
      661 taaaactgcc tataaataca gacaagaaaa gaattactaa aaaaatagct atgatttttc
      721 taaattcatt ttttgttttt ctatctacta ttctattcaa aattatcccc tcctatttat
      781 ttatatgtag ctttggcata atttttataa tatttgtc
//
LOCUS       DAELTA010000339          787 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.344, whole genome shotgun sequence.
ACCESSION   DAELTA010000339 DAELTA010000000
VERSION     DAELTA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.344"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>787)
                     /locus_tag="KPG13_003692"
     rRNA            complement(<1..>787)
                     /locus_tag="KPG13_003692"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAELTA010000340          762 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.345, whole genome shotgun sequence.
ACCESSION   DAELTA010000340 DAELTA010000000
VERSION     DAELTA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.345"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>762
                     /locus_tag="KPG13_003693"
     CDS             <1..>762
                     /locus_tag="KPG13_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG0987327.1"
                     /translation="NNTDKSIDFSISLVSKNQVKVNGLYLNESVYSSYLDFVKNSDGH
                     HNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTLVGKTNLGYVEFICDNNKNIDIYF
                     GEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDISTSLDFSYEPLYGIDRYINKVLIA
                     PDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSSSFEYKWSTEGSDFILV
                     RYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD"
BASE COUNT          302 a           83 c          104 g          273 t
ORIGIN      
        1 ataataatac tgataaaagt atagatttct ctatttcttt agttagtaaa aatcaagtaa
       61 aagtaaatgg attatattta aatgaatccg tatactcatc ttaccttgat tttgtgaaaa
      121 attcagatgg acaccataat acttctaatt ttatgaattt atttttggac aatataagtt
      181 tctggaaatt gtttgggttt gaaaatataa attttgtaat cgataaatac tttacccttg
      241 ttggtaaaac taatcttgga tatgtagaat ttatttgtga caataataaa aatatagata
      301 tatattttgg tgaatggaaa acatcgtcat ctaaaagcac tatatttagc ggaaatggta
      361 gaaatgttgt agtagagcct atatataatc ctgatacggg tgaagatata tctacttcac
      421 tagatttttc ctatgaacct ctctatggaa tagatagata tatcaataaa gtattgatag
      481 cacctgattt atatacaagt ttaataaata ttaataccaa ttattattca aatgagtact
      541 accctgagat tatagttctt aacccaaata cattccacaa aaaagtaaat ataaatttag
      601 atagttcttc ttttgagtat aaatggtcta cagaaggaag tgactttatt ttagttagat
      661 acttagaaga aagtaataaa aaaatattac aaaaaataag aatcaaaggt atcttatcta
      721 atactcaatc atttaataaa atgagtatag attttaaaga ta
//
LOCUS       DAELTA010000341          756 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.346, whole genome shotgun sequence.
ACCESSION   DAELTA010000341 DAELTA010000000
VERSION     DAELTA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.346"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(62..748)
                     /locus_tag="KPG13_003694"
     CDS             complement(62..748)
                     /locus_tag="KPG13_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG0987328.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
BASE COUNT          269 a          107 c           74 g          306 t
ORIGIN      
        1 aatagctgta aaaattatac caacagcaat tattaaataa atagaactaa aagaaaattt
       61 tttaaaattc atttctttca cctaaccaca tatatcccat accatatacc gttttgatat
      121 acttaagatt gtctttttca attttgcttc taatcctact tataacagat gttaaagtat
      181 gttcatctac aaagttagca tctatatccc agagtttttc taaaagtact ttccttgtta
      241 aaatactttt aggattttca gtaagcagtt ttaaagtacg atattctaat ggtgtaaata
      301 taatttgatt tccatttata ttggcagata actcagaaaa gttaatttca agatatccat
      361 ccctatagta atcttgtttt gtttttattg taagatgatt taaaaaagct ttaatttttt
      421 gtttaaatat attgatatta aatggttttg ttatataatc caaagctcca agttcatagc
      481 ctttaatcat atcacattcc atatcattag cagttaaaaa aattattggt atattgtaat
      541 tttcctttat ttcattacat aattcaaacc cacttttatc tggtaaatta atatccaaaa
      601 ttattaaatc aaattcacat tcaaaaatag actttagtgc agatttagct gtaaattttg
      661 aagtaattat atatccatct tcaattaggt tatatgatag agttttattt aatatagaat
      721 catcttctac tactaatatt ttttccatac agttac
//
LOCUS       DAELTA010000342          753 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.347, whole genome shotgun sequence.
ACCESSION   DAELTA010000342 DAELTA010000000
VERSION     DAELTA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.347"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>753
                     /locus_tag="KPG13_003695"
     rRNA            <1..>753
                     /locus_tag="KPG13_003695"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAELTA010000343          691 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.348, whole genome shotgun sequence.
ACCESSION   DAELTA010000343 DAELTA010000000
VERSION     DAELTA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.348"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..665)
                     /locus_tag="KPG13_003696"
     CDS             complement(<1..665)
                     /locus_tag="KPG13_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987329.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          216 a          121 c           37 g          317 t
ORIGIN      
        1 acttttaatt tattgttatt taatctattt tgataaacat tatttatttt attattattt
       61 aaatctttaa aatctaaact tttacattgt tcatcatctt cccatattcc tgcttgttta
      121 tttccatcat ttttatataa tacaccctta ccatttttct tattatctaa aaaatatccc
      181 ttataaatgt cattattggg atatgttaat tttcccaaac cattttttaa tccgtattta
      241 aattcacctg tatatattga accatctcta taagtatatt ttcctattcc atgcatcttg
      301 tcatctttcc ataatccttc atacttttcc ttacttgaaa aaatatatat cccaaatcca
      361 tttttctttc catcaacaaa ctcaccttta tataaatcac cattatcgta tttaaatact
      421 ttttctccct ttaatttctt attgttactc ttatttttat tattattgtt atcctttata
      481 tgtatttctt ctcttctggc ttcaataaaa tctctatcat acctaaatcc gtttcttgat
      541 ttaatttttt gattaatctt cttcttatta ataaatactc tagctagttg agtaaatata
      601 aatacagatg ataagcatat aaatacacat agtattgttg taaaataact agttatattt
      661 tccataaaaa atcacccttt aaaaaattta t
//
LOCUS       DAELTA010000344          688 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.349, whole genome shotgun sequence.
ACCESSION   DAELTA010000344 DAELTA010000000
VERSION     DAELTA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.349"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            132..>688
                     /locus_tag="KPG13_003697"
     CDS             132..>688
                     /locus_tag="KPG13_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG0987330.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDV"
BASE COUNT          258 a           87 c          121 g          222 t
ORIGIN      
        1 actaaacata aagctgaaaa ttattattaa gtttttgtag aaatattata agcatagata
       61 catttttgta acaactacaa taaattttta atactatata ctattcgtat gtcttatggg
      121 aggtatttat tatgtttaaa aaaatagtcg caatagcaag ctctatcatt gtaagtacaa
      181 tatgtctaac tggatgtgat tttaactcta atgacaaaaa aggtgaatct tcttcgtcta
      241 aaaatctgga taaagtaact atagcagaag ttactcactc tgttttttat gctccacagt
      301 atgctgctat tacgaaagga ttctttgaag atgaaggtat aaagattgac cttataaata
      361 cccaaggtgc agataaaact atggcagctt tgatttcagg tgaagcaaac gtaggtttaa
      421 tgggtcctga agcatctata tatgtatata atcaaaacaa aaacgattat gcagtgaact
      481 ttgctcaatt gacaaaaaga gatggtagtt ttctagttgc taggaaaaaa atgcctaatt
      541 tctcttacaa tgatttaaaa ggaaccgaaa tattaggtgg tagaaaaggt ggagttccac
      601 taatgacttt tgaatatgtt ctaaaagaaa aaggtcttac tattggagaa aatctaaaag
      661 caggcaatgt taatgtaaga actgatgt
//
LOCUS       DAELTA010000345          678 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.350, whole genome shotgun sequence.
ACCESSION   DAELTA010000345 DAELTA010000000
VERSION     DAELTA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.350"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(122..520)
                     /locus_tag="KPG13_003698"
     CDS             complement(122..520)
                     /locus_tag="KPG13_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0987331.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          235 a           93 c           70 g          280 t
ORIGIN      
        1 ataaatgtgc ttgaaattag actatacttg tttttaaata atccaatatc catattttag
       61 taatatacta caaaaaaaga aggttaatag atgatgtaaa atcgtatcaa attatgtatg
      121 tttaaaccat tttatcttca ttattattag aggaatgctt ttttaagtct ttatattcag
      181 atatcttaag ttcaagtatt ccttctattt ttattttatc acgttcgttt agttgtctgt
      241 atagatttaa tatcgtcatt tcatcattag taacatgtaa gtaatcttct ttatcttctt
      301 ttacactact attgacattt accttctctt taccatagag aagccagtca gtcgtaacat
      361 taaaataatc agctattgac attagtatat cacaattagg ttttctatct cctgtttcat
      421 acttgcctaa gttttcaaat tttaaaatat ccataagttt gcgctgagta agttttttgg
      481 agtttctcaa ataagcaatt ctttttccta aagtatccac aaaatacact cctttctttt
      541 tatgagtaat gtctaaatga catttaaaat taaaaatata taaatttata atataaaact
      601 actaaattaa agtctaaatg acattttgct taaattaata tgctcataat atgattttaa
      661 catattatag ttgaaaat
//
LOCUS       DAELTA010000346          664 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.351, whole genome shotgun sequence.
ACCESSION   DAELTA010000346 DAELTA010000000
VERSION     DAELTA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.351"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>664)
                     /locus_tag="KPG13_003699"
     rRNA            complement(<1..>664)
                     /locus_tag="KPG13_003699"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAELTA010000347          617 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.352, whole genome shotgun sequence.
ACCESSION   DAELTA010000347 DAELTA010000000
VERSION     DAELTA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.352"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          266 a           41 c           86 g          224 t
ORIGIN      
        1 ttcaagcttg tataatgtaa caattaaacc aaaagactca ttgtatattt gaaattaagt
       61 tttttatagt ttataggtgt tttatgtgga ttacgaatga tatagtattg atacattatt
      121 taatagattc ttatgccaag aaacaattaa tttatagaaa ttcttaattg ttacaatata
      181 aatatctaga taagttataa gatactgata aataatttat aaagtttatt aatctaatat
      241 tgtgtgtgaa tattaagtgg tcaagtagtc taaaaaatga aaaagattta aaaaagtgtt
      301 ttaactaaac acataagtgt taaattcatg tataagtata ggttagatac ataagaaata
      361 attatgtaat atctatcttt gtaaaaaaat attaaatttt gtaatttgtg gataaatatg
      421 tttataatgt ggataatatt taatgtaata agttaaagca atatgaatca actgtttata
      481 atgtggataa tattataaat gtatgaataa ttgcagtatt taaaaacaaa aaaagttaaa
      541 aaagttgttg actctaaaag aaaaagttgg tatagtaata cttgtcctca agaaaagggc
      601 aaacaaataa agcataa
//
LOCUS       DAELTA010000348          593 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.353, whole genome shotgun sequence.
ACCESSION   DAELTA010000348 DAELTA010000000
VERSION     DAELTA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.353"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>593
                     /locus_tag="KPG13_003700"
     CDS             <1..>593
                     /locus_tag="KPG13_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG0987332.1"
                     /translation="ITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISS
                     LYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATV
                     SNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANL
                     LSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNIS"
BASE COUNT          207 a           54 c           78 g          254 t
ORIGIN      
        1 ttataacaat tgtttcttct attatagaaa tatggtcatg taaaaaagtt tttgattata
       61 ctagtcagtc aaagaccgac ttaaaaaaac taaatatgta ttttctcttt tctgtttttc
      121 tcattatttc ttctttatat ataaatatag gagctaatag tagaatcttg atatgtatat
      181 ttgtaatgtt cgttttctat aagtctaact ataaggttaa tatatataaa tgtctggtaa
      241 tctcattttt ttattggtta atattaatag gtataaatgg attaagtatg ttattagtag
      301 caacagtaag caatttggat aatatgaatg ttttacttag tggcagttta tatagatatg
      361 aagcaatatt cttgggaaag ataatgttag gtatattact ttatgtttat aaggcttctt
      421 ttaaaaattt taaaataagt aaaaaagaga tatacatatg tattcctatg atagcaaatt
      481 tattgtcatt ttttgtactt tatgattata ttattaatct tgtaaaaatc tctcctgaaa
      541 gaagaactgc ttttttagta atagttttac tattaatatt atctaacatt tca
//
LOCUS       DAELTA010000349          577 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.354, whole genome shotgun sequence.
ACCESSION   DAELTA010000349 DAELTA010000000
VERSION     DAELTA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.354"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>577
                     /locus_tag="KPG13_003701"
     rRNA            <1..>577
                     /locus_tag="KPG13_003701"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAELTA010000350          554 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.355, whole genome shotgun sequence.
ACCESSION   DAELTA010000350 DAELTA010000000
VERSION     DAELTA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.355"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..>554)
                     /locus_tag="KPG13_003702"
     CDS             complement(<1..>554)
                     /locus_tag="KPG13_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG0987333.1"
                     /translation="EDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKE
                     IIYAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTS
                     DMEKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVC
                     NDLDKFTIEFKHLLDIKNKVNNAN"
BASE COUNT          193 a           84 c           35 g          242 t
ORIGIN      
        1 attagcatta tttactttat ttttaatatc aagtagatgc ttaaattcta tagtgaactt
       61 atctaaatca ttacaaacaa attttattaa ttcaaaatca cttaaattgc tatatatgta
      121 tcttatgtac ttataagaat acattatata aagataaaaa atctccttat atttttcatc
      181 acttaagctt atactttcta aaactacttt agatatagct ttatatactc ttatcttttc
      241 catatctgat gtatttggac gactaattat gtacttatat agtactaaat taaaacttct
      301 ttctaatgct aaaagatatt ttagatattt ctctatccat acatattcca tttcataaaa
      361 aatatctagt aaatcaatct tctcttcaaa agctattttt ataatatatg cataaattat
      421 ttccttttct tcattgagta tttctttaca tttatctata gatacacatt tatttatcct
      481 tatttgattt aaaatgccat aattaccttc aatgtcacaa aggattttat atacatactc
      541 tttttcatcc tctt
//
LOCUS       DAELTA010000351          549 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.356, whole genome shotgun sequence.
ACCESSION   DAELTA010000351 DAELTA010000000
VERSION     DAELTA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.356"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>549
                     /locus_tag="KPG13_003703"
     CDS             <1..>549
                     /locus_tag="KPG13_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRSP domain-containing protein"
                     /protein_id="HBG0987334.1"
                     /translation="YNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGR
                     FGINRREQFRLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKEN
                     QYDIYYHSTTRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVE
                     LNKSENSYLEFIDIIKKTSIKE"
BASE COUNT          256 a           38 c           72 g          183 t
ORIGIN      
        1 attacaacga taattatagt attactaaat tagagactat ttcaagtgat aaagtaaatt
       61 tagtagtaaa tcatatttat gttgatttag aagattatat tggaaataag aaatatgtaa
      121 aatatacagg aagatttgga attaatagaa gagaacaatt tagattaaaa gaaatcgtta
      181 agagagaaag taaaaaaata actatggcca gtaaagaatc tttaaaaaat gaaaaagaaa
      241 agattctatt tttaggtact gaagaattta tgtatatacc tatgctgttt gcaaaagaaa
      301 aagaaaatca atatgatata tactatcatt caacaacaag aagtccaatt attaatattg
      361 ataagaataa ttatcctatt aaaagtaaat ttatgctaaa gagtttttat aatatagata
      421 ttcaaaatta tatttacaat atagataaac ataatttttc taagtgttat ttatttgtag
      481 agctgaataa aagtgaaaat tcttatctag aatttattga tataattaaa aaaacaagta
      541 taaaagaat
//
LOCUS       DAELTA010000352          545 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.357, whole genome shotgun sequence.
ACCESSION   DAELTA010000352 DAELTA010000000
VERSION     DAELTA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.357"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            39..329
                     /gene="cas2"
                     /locus_tag="KPG13_003704"
     CDS             39..329
                     /gene="cas2"
                     /locus_tag="KPG13_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG0987335.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT          226 a           34 c           82 g          203 t
ORIGIN      
        1 ggttttgtat atttttcagt ttaaatatta tattacatat gaaaaagaaa aacaatttaa
       61 attataatta tgtatttgta gtctatgatg taggagaaaa aagagtaagt aaagttttta
      121 aagtgtgtaa aaaatatctc tcgcattttc aaaaatcagt ttttagaggt gaaataactc
      181 catctaagat tatgagctta aaacgtgatt tagaaaatat tatagataaa gataatgatt
      241 ttgtatgtat aataaagttg atgaattcta atacatttga agaagaaata ttagggaata
      301 atagtaattg tgaaaatatg atattataaa tctaattact atatttgtaa aaaataatag
      361 cttattaaag agtagtaaaa ttgggatata atttatgata gagactgcaa gtagattttt
      421 gtgaaaagga tttacttgca gtatttttgt tatgtataac tgttaacatt agtgtacaaa
      481 acattatcaa tagcattttt ctataatgaa gaaatactat tataatggga aaattttatt
      541 aatac
//
LOCUS       DAELTA010000353          496 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.358, whole genome shotgun sequence.
ACCESSION   DAELTA010000353 DAELTA010000000
VERSION     DAELTA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.358"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..>496
                     /gene="tuf"
                     /locus_tag="KPG13_003705"
     CDS             <1..>496
                     /gene="tuf"
                     /locus_tag="KPG13_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG0987336.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAELTA010000354          493 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.359, whole genome shotgun sequence.
ACCESSION   DAELTA010000354 DAELTA010000000
VERSION     DAELTA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.359"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="KPG13_003706"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="KPG13_003706"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="KPG13_003707"
     tRNA            310..398
                     /locus_tag="KPG13_003707"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="KPG13_003708"
     tRNA            410..486
                     /locus_tag="KPG13_003708"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAELTA010000355          464 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.360, whole genome shotgun sequence.
ACCESSION   DAELTA010000355 DAELTA010000000
VERSION     DAELTA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.360"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          205 a           28 c           60 g          171 t
ORIGIN      
        1 acaattattt ataactaaga taataaaaca acaatggtgc taatttatat gtataaatta
       61 attatgaaaa atttatataa gtaaaatata ttaacataaa ggtaaaattt tgtatactaa
      121 aagggaatat ttacagttta atggcagttt taaaataata aattaaagct gtcattttta
      181 tttaaaaatt tttagaaaaa gtacaatagt gttaatgtgt gaattagata aggtgtatat
      241 aatagtttag atgatatgat ataaaatatg ttgtaaatta aatgttttct ttgacaatat
      301 atacacaagg atgataatat ctatatgaat aaaagatagt gtgtaatttg ataatatgta
      361 attataacat tatgatataa ttacaataaa gtatgtaata atctgtagaa atttcaagct
      421 agttatctaa gttttcggat aactaaatct aaacggaata cata
//
LOCUS       DAELTA010000356          411 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.361, whole genome shotgun sequence.
ACCESSION   DAELTA010000356 DAELTA010000000
VERSION     DAELTA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.361"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          178 a           37 c           62 g          134 t
ORIGIN      
        1 aactgtaatc ttacaataga aggattatat atcataaaaa tataaccttg atttatatat
       61 atgaaatata tggtaatttg taggaggaat gtttatgaac atatcaagaa gagctatgaa
      121 gataattgaa ctggcacaaa aaattgctaa taaaagaggt gtaacagtac aagacgcttg
      181 gaatgatgcg atgaaagaat ataaagaaaa atatgaatat gttgcataag atatgcgtta
      241 tattttaaat ataagaaaat ataaaacaaa aaagattgtc tcaaaataag attgtactga
      301 gactcaaaag ttagatttta tacaggaatg gaattttcat tcctgtattt ttaattttac
      361 ataactttaa attcatattc aaatctatat ttaacaaggt tatgctaaat t
//
LOCUS       DAELTA010000357          389 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.362, whole genome shotgun sequence.
ACCESSION   DAELTA010000357 DAELTA010000000
VERSION     DAELTA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.362"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          142 a           44 c           45 g          158 t
ORIGIN      
        1 acatattata gccataataa gtattatttg tattgcatgt ttgctaataa gctgattatt
       61 agacacaata gactcaaagt tatttatgga gttgttttta tagagtttgc ataaagaata
      121 gactggtgtt gtgtatgtaa tttgtaaaat tatacaaata aataaaaaaa acatcaattt
      181 tttttgtaaa ttcctcataa aaatttcctc ccattaaaac acctctattt tattagcaaa
      241 taagtacatc tcaaaagttt ccatttcttg taaaatggat agtttatatc tactttaatt
      301 ttgtaaaatt ttctgctact ataattatag aatgtagcat attcattgtc ttaactattg
      361 atttatatat ttcatatagg tataataga
//
LOCUS       DAELTA010000358          364 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.364, whole genome shotgun sequence.
ACCESSION   DAELTA010000358 DAELTA010000000
VERSION     DAELTA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.364"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KPG13_003709"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KPG13_003709"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="KPG13_003710"
     tRNA            291..362
                     /locus_tag="KPG13_003710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAELTA010000359          309 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.367, whole genome shotgun sequence.
ACCESSION   DAELTA010000359 DAELTA010000000
VERSION     DAELTA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.367"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          134 a           25 c           42 g          108 t
ORIGIN      
        1 aatgtcaaac taaccgtttt agaaattaaa ttatatgtga aaaaagatag cttacattat
       61 tttattatgg aaaataatag taaatggtac aaaatttgat ttttaagatt taaaactttt
      121 ttaaaaagtg ctacaataga ccaaattatt actttctata ctcgttaaac aggaggtggg
      181 actatgaaaa aacataatat tttatttgta tcaactgatg acaaaataaa tatagatata
      241 tcaaaacaat tggaaaatat atttggagaa ttttgtaata tagataactt ggtatatgta
      301 aataggata
//
LOCUS       DAELTA010000360          292 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.denovo.368, whole genome shotgun sequence.
ACCESSION   DAELTA010000360 DAELTA010000000
VERSION     DAELTA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.denovo.368"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
BASE COUNT          124 a           29 c           33 g          106 t
ORIGIN      
        1 ttgaattgtc attaaatatg ccaatagttt tatatattat tgattttatt aaaaaaagag
       61 atatacaaac taataagttg taatctctca aatataaaaa aacaataaat tagctcattg
      121 gattttttag tccataatat aattatagat tattcaccaa acaatctata gaaattgtgg
      181 tattaatgtt taaataacca actttttcta aaataacctt tataggaata tatgagttca
      241 taagtaaaga tagcgaaata agaaaccagt ttggtatgta aaatctagat tt
//
LOCUS       DAELTA010000361         8531 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.guided.155, whole genome shotgun sequence.
ACCESSION   DAELTA010000361 DAELTA010000000
VERSION     DAELTA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.guided.155"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            complement(<1..275)
                     /locus_tag="KPG13_003711"
     CDS             complement(<1..275)
                     /locus_tag="KPG13_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG0987337.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEK"
     gene            complement(947..1762)
                     /locus_tag="KPG13_003712"
                     /pseudo
     CDS             complement(947..1762)
                     /locus_tag="KPG13_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1863..2561
                     /locus_tag="KPG13_003713"
     CDS             1863..2561
                     /locus_tag="KPG13_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG0987338.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            2649..3269
                     /locus_tag="KPG13_003714"
     CDS             2649..3269
                     /locus_tag="KPG13_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG0987339.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            3425..4207
                     /locus_tag="KPG13_003715"
     CDS             3425..4207
                     /locus_tag="KPG13_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG0987340.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            4332..5258
                     /locus_tag="KPG13_003716"
     CDS             4332..5258
                     /locus_tag="KPG13_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG0987341.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            5272..5721
                     /locus_tag="KPG13_003717"
                     /pseudo
     CDS             5272..5721
                     /locus_tag="KPG13_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6081..6488
                     /locus_tag="KPG13_003718"
     CDS             6081..6488
                     /locus_tag="KPG13_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG0987342.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            6490..6720
                     /locus_tag="KPG13_003719"
     CDS             6490..6720
                     /locus_tag="KPG13_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG0987343.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            7110..7313
                     /locus_tag="KPG13_003720"
     CDS             7110..7313
                     /locus_tag="KPG13_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG0987344.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            7391..7744
                     /locus_tag="KPG13_003721"
     CDS             7391..7744
                     /locus_tag="KPG13_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG0987345.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            7816..8448
                     /locus_tag="KPG13_003722"
     CDS             7816..8448
                     /locus_tag="KPG13_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG0987346.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3028 a         1268 c         1412 g         2823 t
ORIGIN      
        1 aatttttctc ccccttctac tacacttcta aatgtgaata ataaagggat atcatgtata
       61 taacttctta attcatagag aacttcctta acttctttaa tattttctac attctcaaaa
      121 aaatcaactc gccactctat aatatccaaa catgcatctt ttaactcttt agcctctttt
      181 ataatatctt ttttattttt cccaataatt ggtacacaaa ttttaggtct accttctcca
      241 atagtaatat tttttacttg aacttttctt ttcatattct cctccataat cagaaattct
      301 ataggacttt aaattctatt ttctgcacaa tttaattata cattaaaaat actatacatt
      361 ataaaaatac taatttaaaa ataattttta ttctaattct taaaacaatc ccacatattt
      421 tagaaaaggt actctataat atacaaacac ttctttttta cctaaattta ttttaacatc
      481 ctataatttt cacaatattc tttaaatttt actcatataa acaatattat tctggatgct
      541 aatagaatta atctataaat catagtataa ttatgcagaa ataactcgaa tttcactact
      601 tatgaagaga ttgaatatta atgaatctaa atcatttgca ttacttaaga gttttgacta
      661 aagttgaaca ccatacaaaa gtagctactc aattgtttat accccaacca agcttaagta
      721 atgttatata gattctcgaa aaagaatttg gtatcttttt atttgaaaaa cttatattaa
      781 atttgattat cttttcataa gttggtaaat gtcgatatat cctgtatcta caaggagtat
      841 cggcattttc tttttggaag atactttgca taagttggca tttaaccgta taagaatgcc
      901 atcaaaagta gtaaataagt agtaaactct agtcatacta ctaggattat aaccgttcca
      961 tttcagctct cgaagaacta aatgaaccat gtgcataata gccaagcgtc atagtaatgt
     1021 ttttatgccc cataatatat tgcaatatat taggactcat tcctttgtta gccatatttg
     1081 tacagagtgt atgcctaaag gtatgtggcg taatctttgg tagcttgtcc tcatgggtct
     1141 tattatactt cttgactaac gctcttaaca tgctttgata atgagttgct tgttttggta
     1201 atccatcttg tttaagaaat aggaagttct ggtaaccatc aacaatgatt ggttctgctt
     1261 tagcgttctt acgccgttcc acgatacgtt caaatgattg ataagctctt tcagtcattg
     1321 gaatttctcg cttaccctgt gtggtctttg gaacttctac ataatagcca atcttagtat
     1381 cactcaacaa ttgatggtct accataatgg ctttattttt catatccaat tctaaagtca
     1441 aaccgcataa ttcagaaatg cgtagtcctg tttctaagag gataacaaca tcatcataat
     1501 atttacaata agtcttatcg ctccccataa aggataataa gcccatttcc tgttctggtg
     1561 ttagagcgat ttttggttct gttgtatctt ccaaaacatc acttaactaa aaattaaatg
     1621 gattctttct aatacagtcg tcttgaattg ccatgaaaaa cgacgctttc agtgagcgct
     1681 tataattgtc aatggattta taagcacacc catcttcact cattcggata gcccagcttt
     1741 tagcgtctga taatttaacc atatcaatgc tttttgcacc tagtgggtca ttgatagtat
     1801 ctatattttg atttataatt tatacatttt tttaaaaaca gacttttaac tgtaaagaat
     1861 ctatgtataa acttagctat gttgtccgtg tatctacaaa gaatcttgac gagctaaaaa
     1921 ggcgttgtga tgaagtcaaa gacttttacg acgacttatc aatcaagctc gtgcgtccct
     1981 ttggggatat gttgggattg catggcgaat ttattcctgt cagcaaacgt tacatcaatg
     2041 attatattca atatgtcact tcggattttt tggctggact tggttttgga gcgacacaaa
     2101 cccttggcga acatgaggat atctatcttg gctacaatct ggacactgga aagaatgtct
     2161 atctaaaacc tgcccttgct agtcaagggg taaagggttc tattaccaat gcgttagcgt
     2221 ctgcgtttat cggttcattg ggtggtggta aattattcag taacaatatg cttgtttact
     2281 atgcggtact attcggcgga caggcggtca tagttgaccc taaagcagaa cgcggcaaat
     2341 ggaaagaaac gctaccagaa atagcccatg agataaacat tgtcaattta accagtaatg
     2401 atgaaaacaa aggattgctt gacccttacg tcattttgag ccgtaaaaag gattctgaaa
     2461 gtttagcgat tgatatccta acattcctta ctggtatctc cagtcgtgac agtgataagt
     2521 ttttgcagaa aaatgacaac tggaaaataa taatggaata gtttctatgt aaatatattt
     2581 ttactatctt acctaactaa ttgaaagatg atataattta atcaaagatt tggtaaggag
     2641 ggatgaatat gcatttaatt gattttatga atgaagatat taaaaagaag cttgttaaaa
     2701 aaacgtataa aaagaaagag actattcttt ttgctgagca agaaaatgaa tatgtaattc
     2761 ttatgattga cggtattgca gaagcattca tcttaaatgt gaaaggaaac atctcaacga
     2821 ttcatcttta tagaagtggt agtttttttg gagaaatgga acaatttaat gatgggaaaa
     2881 aacccgttga aattgtcgct ttcacagatt gtaatgtata taaacttcat agagatgatt
     2941 ttttaaactg gttaagggga gattttgaag ctacaaaatt tctgattcga gaaatatcta
     3001 gtaaattagt tgtaaatgct gaattaattg aagaattatc atctttaagt gttaaagaac
     3061 gacttctgag gtgtatatcc ttacactatt atagaagtga attaaatctg cttaccaaaa
     3121 aacagcttgc tagcgaagta aacgctccaa ttagaagcat taatcgtgct ataaatgaat
     3181 gtgtacaaga acatctaatt gcttatgaaa acaaaagatt tagtgtaatt aatcagcgag
     3241 aggtattaaa gaatcttcct acaacataag tataatttca aaaaagtatt gaataataat
     3301 tattcaatgc ttttttattt cgggtcaagt gacctatttt tttttatttc ttcattatat
     3361 aatactccta tgcataatta tgttggattg atagctaata aaagacagtg agaggtgtta
     3421 tattatgact aaaacacaac agaaaatgat acaaaacgta ttagaatttt caaaaagaga
     3481 tagtaggata ataggacttg caattagcag ttcttatatc acaaaatcgt tagatgaata
     3541 ttctgatttg gattttctga ttgtagcaga tgataaatcc tatgaagaag ttatcgaaga
     3601 gagattactt attgtggaac agtttggaac attagtttct gcttttactg gcgaacacgt
     3661 tggagaacct cgattgataa tttccctcta tgattcccct gttcttcatg ttgattttaa
     3721 gtttacaacc ttagatggtt tactagatag ggttgaagat tcagctattc tctatgaaga
     3781 aaacaactgc ataacagaaa tttattctaa aaaagaagca tacttcccaa ctccagactt
     3841 acagtggatt gaagatagat tttggatttg ggttcattac gcttgcacta agattggacg
     3901 tagagaacta tttgaaacaa ttgatttttt atctttttta agacaatcag tacttgcccc
     3961 attaatacag cttctcttag gtaaattacc tcgtggagtt agaaaaattg aattggacgc
     4021 accccaatat attgaagcat tagaaaagac ggttgctaac catgaatata aaagctgtgt
     4081 taatgcattg caggctacta tcaacctgta tcttgagtta agagaggaat tatcacaaag
     4141 tcctattgtc cgaaaggttg aagctgaaag ggttgcatta cagtattttc aagatttaat
     4201 caaataaacc caaagtaaaa aaatattgaa tagtaaatat tcagtgtttt ttcatttttg
     4261 gtcaaatgac ctagattagt tgttgtaaca attctataat tattttgaaa gtaacagtat
     4321 ggaggataac tatgagtgat attttaagta gaattacaaa taaaattggg gataagaata
     4381 tagttgataa gttatcagct ctatcaaaat cagatttgaa ctcacttcta ttagaagtgt
     4441 ttgatagaca agcaaatact ttaactgcaa ctgatattct aaagtcatac caactaaata
     4501 gatttactat ccctagcggt atagatccag aagaaattca tgctttggaa tcaaaattat
     4561 tgagaaaagc attcaatatg gatatcaaga cgattatgct ttctccctct gccccattag
     4621 gtagctgtag cgtttttggc tccgtagatc aatataacgt agtaagtgct ctacgaggga
     4681 cagaaacact tgcagacccc tcaaatatgt tggcaataat tattgctgat aaattaaaaa
     4741 gaaaagaaga aaataatacc atacctatcc acatggctac tactgctagg gtaataagag
     4801 cacaaaattt tgtaggtaaa ggtttatatt cacattttgg cttatattgt atggtttcat
     4861 ctggtataga tacagggtct tacacttgtg aaaaaatgtt gttagaaaag cacttaaact
     4921 attataaaga tatattaagt gaaatagaaa atgttcgttt ctctattatt ctacgcaaaa
     4981 gaaacggata caaagataat gacggatttt tcgacagaat ggttgaaact atcaaactta
     5041 tttttcctaa tatagagtta tcaattcata atgatgatgt tgataataat tattataaag
     5101 gtatcaattt caaattatcg gtcaaacaag gaaatgaaac cgttgaaata gttgacggtg
     5161 gatttgttga ctggacttat caaatgcttg gaaataaaag agaacgctgt ttgataagtg
     5221 gtattggctt agaaagattt cttgtagctg tttcataaaa ggagaagaaa aatggaactt
     5281 atagagtgga attatgctta tattaccgat ttagttgagt ttgcgaatga taaagaaatt
     5341 gcaaagaatc ttcgtgacaa cttcccccac ccttataggg agcaaaacgc tagagatttt
     5401 gttagttttt gtttggcaac ttcagatgtt aaacaactta gctatgctat tttttatcaa
     5461 gaaagggcta ttggaagtat cagcctaaca tttgaagaag atacctctat taataaaaat
     5521 gctgaattag gttactggct tgctaggaac tattgaggca aagggatagc aacaaatgca
     5581 gtaaaagaaa tatgcaaaat tgcctttgaa aaatacaacc ttaacaaaat tcatgctaat
     5641 gtatattcct acaatagaga gtcaagtaaa gtattggaaa aatgtggctt taagattgaa
     5701 gaatactgtc acaattcata ataaagaata gaaacatgat tgattgccat gtatatagtt
     5761 taattcgacc gcaatagcat ttattagaat taacctactt tttaataaca gaataagttt
     5821 ttcccctcta ttaatttctt atgcatcttt gtatttttat gaaaagctag ccaatgcata
     5881 tttccataca tcaatatggt ggtggcttac aaccaatata aattaaatac gttttaaaga
     5941 atacacattt ttgccctata ggcagagtgt gtattttttt ctcttttttt tgaaatttat
     6001 cgaagattta acgccctccc accgtgatag gagtaagcaa ggttatttaa ctccttgcga
     6061 tggaaagagg gtgaaatctt atggttgatg aaaagacaat tgaacatcaa ttcgacagtt
     6121 tctgtaaaac agttttgcgt aactatgcta gagatattta tgacgaaaat aaacgcagaa
     6181 atgattattt ggtatctctc gaatctttgt cacttgcaga attaagtaaa ttgagtattt
     6241 tagatgatta tgattctaac tacatctgta tggtttctta cgactacaac atacgaattg
     6301 aagatgtatt aatggcgcaa gcaatcggaa aacttacaaa aagaaaacaa gatataattc
     6361 tactttcatt ctttttcaac atgacaaatg ctgacatagc aacattgatg gatttagcag
     6421 aaaataccat acattatcac aaaacaaatg cactaaaaga gttgaaagaa ctcatgcagg
     6481 aacattgaaa tgaaacctac taaagaagta ttacctttta caattatttt acttgctgta
     6541 catggcgatg taaatgcaat aaacgtaata cttaaacatt ttgaacacta tatcattcgt
     6601 ctttcacaga aaactttatt tgatgaatac ggaaatccct atattcatgt agaagaagaa
     6661 acaaagcgaa tgctaggaac aaaactaatc actgctattt tgagatttaa tttgtcttaa
     6721 tctcgccctc tctccatgta tcaagctatt tggtacatgg agaatcacta attatttgtc
     6781 tataaaagcc cacaagctat tgtgattttt tataggcaaa tcaatgccga ctgttctttg
     6841 acaactgaat aaagtagata gatacgtttg tgatacagat agccaacaaa tactatacgc
     6901 catgacctta atcaagagag ctactatata gtgatttgtg aataaccttt ggaacggttg
     6961 ttttgccgta acctgtacca cagtataatg atacacctgt atgacacggt tctgtccata
     7021 agaatgggat tggttgaaat accaatggag cttatcaaag ccatctatct acttctgaac
     7081 tacgaacata gaaaggagtg atacattata tgaacaatac tgatgtacct atttgggaaa
     7141 aatatacctt gacgattgaa gaagcgtcaa aatactttcg tattggagag aaaaaattac
     7201 gtaaacgtgc tgatgaaaac cttgattctg gctgggtcat tatgaatagc aatcgcattc
     7261 aaataaagag aaaacaattt gagaaaatcg ttgatacact tgacgttatc taatgaaatt
     7321 gagccttata tgttataata ttcatatgac gtatcaagct caattcggtt attgagagga
     7381 gcttcttatc atgtcaactg aaaaaagacg agataataaa gatagaattt taagaactgg
     7441 agagagccag agaaaagatg gaagatacgc atataagtat gtatattcat ttggtaaacc
     7501 acaatttgtt tactcttgga aacttgtacc tactgataaa cccccaaagg gaaaacgtga
     7561 tgatatatca ttaagggata aggaaaaagc aatacaaaat gaccttgatg atagcattga
     7621 cactattggt aagaaaatga cagtttgtca gttatacgct aaaaagaatg gactaagaaa
     7681 gaatatccga cttaacacta aaaaaggacg ccattatctt atgaagattc ttaacaagga
     7741 gtgattctgt gaagagatgt attgtagtag tggaatagtc aaaaacataa tatatattaa
     7801 aggagagatt taaatatgac tattccaagt tcaaataagg tatatccaag aagtaatgat
     7861 tatcaaactg tatatcttaa aaatgtaata acaagggaaa atatagaagt tggagattac
     7921 acaatatata atgactttta taatgaccca agagattttg agaaaaacaa tgtattgtat
     7981 cattacccta taaacaatga taaattaata attggtaagt tttgctcaat agcatgtaat
     8041 gcaaaatttc taatgactag tggaaatcac tcaatgaaat caaaatccac atatactttt
     8101 cccatctttt atgaagaatg gaaactcgat atcaaccata taacggacgc ttgggacaat
     8161 aagggtaaca ttataattgg aaatgatgtg tggataggtt atgattctat aattatgtca
     8221 ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag cagtagttac taatgacgtt
     8281 ccaccgtatt ctattgttgg aggagtacct gcaaaaatta taaaaaagag atttaataat
     8341 gatacaattt caaaattatt agaaattaaa tggtgggatt ggccatataa aaaaattcaa
     8401 tcaaacatta agcatattca atcaggaaat attgataaat tagtttaata ttttataatt
     8461 tatttatatt aaactttact ttaataatca ttttataaga gctttttcaa aatttgtata
     8521 ttattttaat a
//
LOCUS       DAELTA010000362         5346 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.guided.207, whole genome shotgun sequence.
ACCESSION   DAELTA010000362 DAELTA010000000
VERSION     DAELTA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.guided.207"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            246..863
                     /locus_tag="KPG13_003723"
     CDS             246..863
                     /locus_tag="KPG13_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG0987347.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="KPG13_003724"
     CDS             971..3502
                     /locus_tag="KPG13_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG0987348.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="KPG13_003725"
     CDS             3936..4637
                     /locus_tag="KPG13_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG0987349.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4627..>5346
                     /locus_tag="KPG13_003726"
     CDS             4627..>5346
                     /locus_tag="KPG13_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HBG0987350.1"
                     /translation="MKSKNIKVNPDFTQVRNKIFMRTALMAFIAIIFIWILYSFILYG
                     KFSIWIVSFFENAFRMEYKAALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTK
                     YFSEINQGIDSLIKEDVGEVALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELI
                     VYLAHDLKTPLTSVIGYLTLLRDESQISEELRKKYLSISLDKAERLEDLINEFFEITR
                     FNLSNISLEYSTVSLTRMLEQL"
BASE COUNT         2099 a          521 c          990 g         1736 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caacta
//
LOCUS       DAELTA010000363         5229 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.guided.211, whole genome shotgun sequence.
ACCESSION   DAELTA010000363 DAELTA010000000
VERSION     DAELTA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.guided.211"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KPG13_003727"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KPG13_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG0987351.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="KPG13_003728"
     CDS             1575..2258
                     /locus_tag="KPG13_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG0987352.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="KPG13_003729"
     CDS             2255..4915
                     /locus_tag="KPG13_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG0987353.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..>5229)
                     /locus_tag="KPG13_003730"
     CDS             complement(5096..>5229)
                     /locus_tag="KPG13_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG0987354.1"
                     /translation="NASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFYT"
BASE COUNT         2024 a          599 c          895 g         1711 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcaattagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcatttt
//
LOCUS       DAELTA010000364         5205 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.guided.213, whole genome shotgun sequence.
ACCESSION   DAELTA010000364 DAELTA010000000
VERSION     DAELTA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.guided.213"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            <1..218
                     /locus_tag="KPG13_003731"
     CDS             <1..218
                     /locus_tag="KPG13_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBG0987355.1"
                     /translation="IEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGR
                     LREKIHEPSKNPKFIKTVWGVGYTIEK"
     gene            268..1350
                     /locus_tag="KPG13_003732"
     CDS             268..1350
                     /locus_tag="KPG13_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG0987356.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            1611..2711
                     /gene="vanG"
                     /locus_tag="KPG13_003733"
     CDS             1611..2711
                     /gene="vanG"
                     /locus_tag="KPG13_003733"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0987357.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            2708..3514
                     /locus_tag="KPG13_003734"
     CDS             2708..3514
                     /locus_tag="KPG13_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0987358.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            3532..>5205
                     /gene="alr"
                     /locus_tag="KPG13_003735"
     CDS             3532..>5205
                     /gene="alr"
                     /locus_tag="KPG13_003735"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG0987359.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVR"
BASE COUNT         1900 a          599 c          909 g         1797 t
ORIGIN      
        1 cgattgaatt ttctattctt tggtatttat gtgaacatca aggaaaagtg gttccttctg
       61 aagaattgtt tgaagctgta tggggagaaa aatatcttga taataataac acagttatgg
      121 ctcatatagg gcgattacgt gaaaaaattc atgaaccttc taaaaacccg aaatttataa
      181 aaactgtatg gggagtaggt tatacaattg aaaagtaaaa atattaaagt caatcctgac
      241 tttactcaag tgagaaataa aatttttatg agaacagctt taatggcatt tatagccatt
      301 attttcatat ggattttata ttcttttata ttgtatggta agttttctat ctggatagta
      361 tcattctttg aaaatgcttt tcgtatggaa tataaagctg cattgaattt atatcagcag
      421 attttccgta atagcatgga gctcttcatg atactagcta tggtgattgt gttttttata
      481 atattccggg tttatttaaa ttggtttaca aaatatttta gtgaaataaa tcaaggtatt
      541 gactctctta tcaaagaaga tgttggagaa gtggctttat ctccagagtt attggcaata
      601 gaaaagaaaa taaactcaat taaacatata cttgaacaac gtaaatttga gacacaaatg
      661 gcagaacaac gtaagaatga acttattgtt tatcttgcac atgatttaaa aacaccactt
      721 acatcagtta tagggtatct gacactactt cgagatgaaa gtcagatttc tgaagaactg
      781 cgaaaaaaat atttatctat ttcgttggat aaggcagagc gtcttgaaga tttaattaat
      841 gaattctttg aaattacaag gtttaattta tcaaatatat cacttgaata cagtacagta
      901 agcttaactc gtatgcttga acaactaaca tatgagttta aacctacttt gatgagtaag
      961 aatttaaaat atacactgaa tgttacacca aatatgatga taaggtgtga tgttaataaa
     1021 atgcaacgtg tatttgataa cttattacga aatgctgttt attatagttt tgaagatact
     1081 actattgaaa taacagctgt acaggaggat gattatataa agcttaaatt tataaatcat
     1141 agcaatacga ttccagagga aaaacttgag cgtatttttg aacaatttta caggttggat
     1201 acatctcgta attcaaacag tggtagagct gggttgggac ttgctatagc taaagaaata
     1261 gtggagcttc ataacgggac aataacagct catagcgaga acaatataat tatctttaag
     1321 gttattattc cgattgtagg aaatttgtaa gaatttaagt ataaaaaaat aggaaattct
     1381 ttaggataaa taaataaaga gcactcttgt tattgatata attactgtat caaaacaaga
     1441 gtgcttttat tttgatacag taattatatc aatagaaaaa tgtgaaaata tattatgtgg
     1501 aacagatgca attgtaagtg aaggttgatt ggatttgaaa tagaaaaagt attttaacat
     1561 gtttctgtag attgtataaa aataaataaa tcaacaaagg agaatcaaag atgaataata
     1621 aaaaaattgc agttatattt ggtggaaact caacagaata tgatgtatca ttacaatcag
     1681 cttttgcagt acttgagaat ttgaatacgg aaaaatatga tattatacca attggaatta
     1741 caagggaggg taattggtat cattatattg gagattataa taatatccag aataacactt
     1801 ggtttgaaag ggatactgat ataattccag ttattgtttc gcagaaccgt gtcaaaaatg
     1861 gtattattga atttttaatt cctgatgctg tttctcagaa tcatattcca aatagtgcta
     1921 ttgaatcatg tgaaaatgat attaaataca ctaaacttga ttttgtgttt cctgtactgc
     1981 atggaaaaaa tggagaagat ggaacagtgc aggggttatt tgaattggca ggtatacagg
     2041 ttataggatg tggtactctt tcttcagcat tatgtatgga caaagacaaa tcacataaac
     2101 ttgttcattc agaaggtatt aaagtaccaa aatcaatatt actaaaatac tcagataaat
     2161 ttaatgtggc tgaactgtca aaagacttaa cttatccatt atttataaaa cctgtgcgtg
     2221 caggttcatc atttggtatt agtaaaattt ataaaaaaga agagttacaa gtagcagtag
     2281 acttggcttt tgaacatgat aatgaagtaa taattgagga aaatattgat ggttttgagg
     2341 tagggtgtgc agttttagga aacgatgact tacttgttgg aagagtagat gaaatagaat
     2401 tgtcaaaagg attttttgat tatacagaaa aatatacatt gaaaagttca aaaattcata
     2461 tgccagctag aattgatttg gattgtgaga aaagaataca agagacagca aaaattattt
     2521 atagaactct tggatgttct ggatttgcta gagtagatat gtttttaact ccatctggtg
     2581 aaattatttt taatgaagtc aatacaattc ctggctttac ttctcatagt cgttatccaa
     2641 atatgatgaa aggtattgga ttgtcttttg aagaaatatt ggacaagata attgaggtgt
     2701 acataaaatg atgaataata ttttattaga taaaaataaa gaagataaaa tgacaaatac
     2761 tatcttattg actaaagata aagtttatga tgggaatcta attctagtaa atgctttttt
     2821 accagttaaa acatctgaag atatagattt aattcctgta gatacaaggt ttccaagtat
     2881 tcttatgaaa cgtgaagcta caaacatttt acaaaatatt ttaaaaagta tatgtggaat
     2941 aaatgaaata gtacctgtaa gtggatatcg tactgcagag gaacaacaag atatatattc
     3001 aagttctctg cgtgacaatg gaaaagactt tactaaaaag tttgtagcac ttccaaatca
     3061 cagtgaacat cagacagggc ttgctattga tttagggtta aagaaagaag ttatagattt
     3121 tatctgccct gaatttcctt ataatggtat ttgtaatgag tttcgtaagg cagcactaca
     3181 ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa ataacaggaa tatctcaaga
     3241 accatggcat tttcgttatg tgggatatcc gcattctgag attatcaata aaatgaatct
     3301 cgctcttgaa gaatatatag aatatattaa gagattttcg tatacaaatt gtttaaaaat
     3361 agaagataaa gaaaaaatta tagaaatctt ttatgtgcct attcatacta aagaagaagt
     3421 gagaatctct atacctgaac aatgtgtata tcaaatatcg ggtaacaata aggatggttt
     3481 tattgtgaca ttttggagaa atagaaagga ctaagaaatg gaggaacaag aatgattagg
     3541 aataaatctt atgcagggct tgattatttt aggattatag ctgcattact tataattgca
     3601 attcatacat ctccattatt atcatatagt aaaatggctg actttgtgtt aactagtata
     3661 attgctcgta ttgctgtgcc attctttttt atgctatcag gcttttttct aatttcaaca
     3721 tataattata attgtaataa actgaaatct tttctaaaaa atgtattatt tatttacggg
     3781 atagctattg taatttatat tcccttaaat atttacactg gatatttttc catgaataat
     3841 ttagtgccca atattattaa ggacattgta tttgatggga ctttatatca tttatggtat
     3901 ctacctgcat caattattgg tgcaagtatt gcatactttc ttgtaaaaag actaggttta
     3961 aagtactcac tagtaatcac tattgttatt tatgtaattg gtatgtttgg tgatagttat
     4021 tatggttttt tagaaaaaat accactatta aagaatttat atgggtatat ttttgaagta
     4081 tcagattata cacgaaatgg tatatttttt gcacctgtat ttttcatact tggtggaata
     4141 atatctaata aatctaatta tatgtcaact aaaaatagct tgattggatt tcttatatca
     4201 ttcttactta tgttatgtga gggaatgttt ttacataaac taggggtaca gcgtcatgat
     4261 agcatgtata ttatgttatt gccatgtatg tatttcttat ttacagcctt aacatttttg
     4321 aaaggttctc gagctaggtt attgcgaaca tcatcattaa ttatttatat tatacatcct
     4381 atgatgattg ttgttattcg tatgttttct aaaatattcg gaatacaaac tgtcttggtt
     4441 gacaatagcc ttattcatta tatagtggta agtatagcat cttttatagc ttctataatt
     4501 attatattga ttcttaaacc tctaaaaaaa gacttgaaag gagaacataa ggtaaatacc
     4561 aatagagctt ggattgaaat tgatttaaat aatttggaac ataatgtaaa gatgatacaa
     4621 aaagctatgc cagaagattg tgaattgatg gcagttgtaa aagctaatgc ctatggtcat
     4681 agtgcttttg aagtagcgac ttgtgcaaac caaataggtg taaaagcatt tgcagtagca
     4741 acaatagatg aggggattga tttacgtcga tatggaatac taggagaaat attgattctt
     4801 ggatatacta acccaataag ggcaaaggaa cttcataaat acaatcttac acagagtgtt
     4861 attgacctta atcatgctat ctctttaaac aaacagaaat acaacataaa atcacacatt
     4921 aaaattgata caggtatgca cagactgggt tttgatgtta atgatgtgag gtcaatttta
     4981 aaggcattta aattaaaata cttagatata tgtggcatat ttacacactt gtgtgtttca
     5041 gatagtctga aagatgaaga tgtagacttt acaaataaac aaattgaatg tttttataag
     5101 ttaattgatt ttctgttaaa aaaaggtata aaaattccta aagtacatat tcaaagtagt
     5161 tatggttttt ttaattatcc caatcttaag tgtaattatg taaga
//
LOCUS       DAELTA010000365         4918 bp    DNA     linear   BCT 08-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate M2017013712
            SAMN15907638-rid12312853.guided.220, whole genome shotgun sequence.
ACCESSION   DAELTA010000365 DAELTA010000000
VERSION     DAELTA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15907638
            Sequence Read Archive: SRR12520745
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001050597.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001050597.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2021 08:16:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,656
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 17 of 49
            Pseudo Genes (incomplete)         :: 21 of 49
            Pseudo Genes (internal stop)      :: 21 of 49
            Pseudo Genes (multiple problems)  :: 9 of 49
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15907638-rid12312853.guided.220"
                     /isolate="M2017013712"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Minnesota"
                     /collected_by="CDC, Emerging Infections Program"
     gene            61..423
                     /locus_tag="KPG13_003736"
                     /pseudo
     CDS             61..423
                     /locus_tag="KPG13_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            684..1784
                     /gene="vanG"
                     /locus_tag="KPG13_003737"
     CDS             684..1784
                     /gene="vanG"
                     /locus_tag="KPG13_003737"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG0987360.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            1781..2587
                     /locus_tag="KPG13_003738"
     CDS             1781..2587
                     /locus_tag="KPG13_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG0987361.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            2605..4743
                     /gene="vanT"
                     /locus_tag="KPG13_003739"
     CDS             2605..4743
                     /gene="vanT"
                     /locus_tag="KPG13_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG0987362.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         1797 a          552 c          861 g         1708 t
ORIGIN      
        1 acatatgagt ttaaacctac tttgatgagt aagaatttaa aatatacact gaatgttaca
       61 ccaaatatga tgataaggtg tgatgttaat aaaatgcaac gtgtatttga taacttatta
      121 cgaaatgctg tttattatag ttttgaagat actactattg aaataacagc tgtacaggag
      181 gatgattata taaagcttaa atttataaat catagcaata cgattccaga ggaaaaactt
      241 gagcgtattt ttgaacaatt ttacaggttg gatacatctc gtaattcaaa cagtggtaga
      301 gctgggttgg gacttgctat agctaaagaa atagtggagc ttcataacgg gacaataaca
      361 gctcatagcg agaacaatat aattatcttt aaggttatta ttccgattgt aggaaatttg
      421 taagaattta agtataaaaa aataggaaat tctttaggat aaataaataa agagcactct
      481 tgttattgat ataattactg tatcaaaaca agagtgcttt tattttgata cagtaattat
      541 atcaatagaa aaatgtgaaa atatattatg tggaacagat gcaattgtaa gtgaaggttg
      601 attggatttg aaatagaaaa agtattttaa catgtttctg tagattgtat aaaaataaat
      661 aaatcaacaa aggagaatca aagatgaata ataaaaaaat tgcagttata tttggtggaa
      721 actcaacaga atatgatgta tcattacaat cagcttttgc agtacttgag aatttgaata
      781 cggaaaaata tgatattata ccaattggaa ttacaaggga gggtaattgg tatcattata
      841 ttggagatta taataatatc cagaataaca cttggtttga aagggatact gatataattc
      901 cagttattgt ttcgcagaac cgtgtcaaaa atggtattat tgaattttta attcctgatg
      961 ctgtttctca gaatcatatt ccaaatagtg ctattgaatc atgtgaaaat gatattaaat
     1021 acactaaact tgattttgtg tttcctgtac tgcatggaaa aaatggagaa gatggaacag
     1081 tgcaggggtt atttgaattg gcaggtatac aggttatagg atgtggtact ctttcttcag
     1141 cattatgtat ggacaaagac aaatcacata aacttgttca ttcagaaggt attaaagtac
     1201 caaaatcaat attactaaaa tactcagata aatttaatgt ggctgaactg tcaaaagact
     1261 taacttatcc attatttata aaacctgtgc gtgcaggttc atcatttggt attagtaaaa
     1321 tttataaaaa agaagagtta caagtagcag tagacttggc ttttgaacat gataatgaag
     1381 taataattga ggaaaatatt gatggttttg aggtagggtg tgcagtttta ggaaacgatg
     1441 acttacttgt tggaagagta gatgaaatag aattgtcaaa aggatttttt gattatacag
     1501 aaaaatatac attgaaaagt tcaaaaattc atatgccagc tagaattgat ttggattgtg
     1561 agaaaagaat acaagagaca gcaaaaatta tttatagaac tcttggatgt tctggatttg
     1621 ctagagtaga tatgttttta actccatctg gtgaaattat ttttaatgaa gtcaatacaa
     1681 ttcctggctt tacttctcat agtcgttatc caaatatgat gaaaggtatt ggattgtctt
     1741 ttgaagaaat attggacaag ataattgagg tgtacataaa atgatgaata atattttatt
     1801 agataaaaat aaagaagata aaatgacaaa tactatctta ttgactaaag ataaagttta
     1861 tgatgggaat ctaattctag taaatgcttt tttaccagtt aaaacatctg aagatataga
     1921 tttaattcct gtagatacaa ggtttccaag tattcttatg aaacgtgaag ctacaaacat
     1981 tttacaaaat attttaaaaa gtatatgtgg aataaatgaa atagtacctg taagtggata
     2041 tcgtactgca gaggaacaac aagatatata ttcaagttct ctgcgtgaca atggaaaaga
     2101 ctttactaaa aagtttgtag cacttccaaa tcacagtgaa catcagacag ggcttgctat
     2161 tgatttaggg ttaaagaaag aagttataga ttttatctgc cctgaatttc cttataatgg
     2221 tatttgtaat gagtttcgta aggcagcact acattatggt tttatagagc gttatcagtg
     2281 taaaaaagaa gaaataacag gaatatctca agaaccatgg cattttcgtt atgtgggata
     2341 tccgcattct gagattatca ataaaatgaa tctcgctctt gaagaatata tagaatatat
     2401 taagagattt tcgtatacaa attgtttaaa aatagaagat aaagaaaaaa ttatagaaat
     2461 cttttatgtg cctattcata ctaaagaaga agtgagaatc tctatacctg aacaatgtgt
     2521 atatcaaata tcgggtaaca ataaggatgg ttttattgtg acattttgga gaaatagaaa
     2581 ggactaagaa atggaggaac aagaatgatt aggaataaat cttatgcagg gcttgattat
     2641 tttaggatta tagctgcatt acttataatt gcaattcata catctccatt attatcatat
     2701 agtaaaatgg ctgactttgt gttaactagt ataattgctc gtattgctgt gccattcttt
     2761 tttatgctat caggcttttt tctaatttca acatataatt ataattgtaa taaactgaaa
     2821 tcttttctaa aaaatgtatt atttatttac gggatagcta ttgtaattta tattccctta
     2881 aatatttaca ctggatattt ttccatgaat aatttagtgc ccaatattat taaggacatt
     2941 gtatttgatg ggactttata tcatttatgg tatctacctg catcaattat tggtgcaagt
     3001 attgcatact ttcttgtaaa aagactaggt ttaaagtact cactagtaat cactattgtt
     3061 atttatgtaa ttggtatgtt tggtgatagt tattatggtt ttttagaaaa aataccacta
     3121 ttaaagaatt tatatgggta tatttttgaa gtatcagatt atacacgaaa tggtatattt
     3181 tttgcacctg tatttttcat acttggtgga ataatatcta ataaatctaa ttatatgtca
     3241 actaaaaata gcttgattgg atttcttata tcattcttac ttatgttatg tgagggaatg
     3301 tttttacata aactaggggt acagcgtcat gatagcatgt atattatgtt attgccatgt
     3361 atgtatttct tatttacagc cttaacattt ttgaaaggtt ctcgagctag gttattgcga
     3421 acatcatcat taattattta tattatacat cctatgatga ttgttgttat tcgtatgttt
     3481 tctaaaatat tcggaataca aactgtcttg gttgacaata gccttattca ttatatagtg
     3541 gtaagtatag catcttttat agcttctata attattatat tgattcttaa acctctaaaa
     3601 aaagacttga aaggagaaca taaggtaaat accaatagag cttggattga aattgattta
     3661 aataatttgg aacataatgt aaagatgata caaaaagcta tgccagaaga ttgtgaattg
     3721 atggcagttg taaaagctaa tgcctatggt catagtgctt ttgaagtagc gacttgtgca
     3781 aaccaaatag gtgtaaaagc atttgcagta gcaacaatag atgaggggat tgatttacgt
     3841 cgatatggaa tactaggaga aatattgatt cttggatata ctaacccaat aagggcaaag
     3901 gaacttcata aatacaatct tacacagagt gttattgacc ttaatcatgc tatctcttta
     3961 aacaaacaga aatacaacat aaaatcacac attaaaattg atacaggtat gcacagactg
     4021 ggttttgatg ttaatgatgt gaggtcaatt ttaaaggcat ttaaattaaa atacttagat
     4081 atatgtggca tatttacaca cttgtgtgtt tcagatagtc tgaaagatga agatgtagac
     4141 tttacaaata aacaaattga atgtttttat aagttaattg attttctgtt aaaaaaaggt
     4201 ataaaaattc ctaaagtaca tattcaaagt agttatggtt tttttaatta tcccaatctt
     4261 aagtgtaatt atgtaagaat tggaattgca ttatacggga tattaagttc tccaaatgat
     4321 acgactaaat tgcaacttga tttacgacct gttttatcct taaaatcaca agtaattctt
     4381 attagaaaaa tacaaaaaga tgatagtttt ggatatggaa gagtctttat tgcaagcaga
     4441 gatagcgtaa ttgctatatt atctattggc tatgccgatg gtatacctag aaatttatcc
     4501 tgtggaaaaa gttacgtaat aataaatggt tgtagagcag caatagtggg tcgaatttgc
     4561 atggaccaac ttgctgttga tattacagat atacctaatg tagaagttgg taatacggca
     4621 ataataatag gaagagataa tttatctgaa ttatctgctt ctgaagttgc aaataactca
     4681 tgtagtatat cgaatgaatt attgagcaga gtaggaagaa ggttaaatgt tataaagaag
     4741 taactcaatt aaatagtaat atttgttgta ctgtgtaatt tattttactc tgactagaca
     4801 tttttagtaa tattatgtct aatatatatt ttttataact tacatgacaa tttaagttat
     4861 aaaaaggtga tgtttcagat ttttattttg aaacatcacc ttttttgtta taatatat
//
